LOCUS       DAOQXS010000001        22120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_2707_length_22120_cov_4.695491, whole genome shotgun sequence.
ACCESSION   DAOQXS010000001 DAOQXS010000000
VERSION     DAOQXS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_22120_cov_4.695491"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..289
                     /locus_tag="PKM34_00005"
     CDS             95..289
                     /locus_tag="PKM34_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81990.1"
                     /translation="MNVRYKYPFNENPLFLDSMLKARKKLNMHVVVLKKILTIMYYLG
                     GEAVILVLSNDLGVVISYYI"
     gene            876..2363
                     /locus_tag="PKM34_00010"
     CDS             876..2363
                     /locus_tag="PKM34_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ81991.1"
                     /translation="MFTRNNSEGVNDLSDIPFTHDELIDGRYLYAQIKHSSRKKLIVD
                     PYYTSENSIQFYKEYSSRKFFLNQIPFYLFTGGVLLMFFYFIGFYFIYSDRLFIYYSL
                     YLLALILYIGARAAFIQEEFRMALPHAIHIYNDVMQVFVNIFYLKFAQQVLNAKTDFP
                     KLNIAIRYALTLLITIVAFQFAILVFKPFSAAGPFIIEFQRYFMIVFSLASYVYIIRH
                     YKKKVVLYLVGGSLFYLSGALLALYLWEIKYMMLGTSIEVFIFSLILGYRTKLVEQEK
                     KSIEAEMTQVKLTALKAQMNPHFIFNSLNSIRAYVISNETKKASDYLNKFARLIRLIL
                     YYSSRDFILLKEELETLRLYIELEQMRYRDDFGFELKISPAVDIGKCLVPPLILQPYV
                     ENAIGHGLAPKTGEKKLWLEIAKKDHSVVFAIRDNGVGRRFSKKVNHIKDQKHESMAM
                     ELTRKRINLAGENLSADNKIEIIDIEESGQSLGTEVRFKLPIQTT"
     gene            2375..3094
                     /locus_tag="PKM34_00015"
     CDS             2375..3094
                     /locus_tag="PKM34_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ81992.1"
                     /translation="MKLTAAIIDDEIHAIETLRYDLDENHRNDVKVLFSTTNPVEGVK
                     RIRTDKPDLLFLDVDMPGLTGLELIQLIDDLPVRIIFTTAHTEYAIKAVETVASGYLL
                     KPVQPDDLQRVIEKAMDTLKPAAFETPVKDKIPVCDNDGIELVPCADIIYCKSDSNYC
                     TLVLYGKRRITASKTLKHFEELLGNDQFIRVHKSFLINRNHIKKYLRKGGGELVMSNN
                     DVVPLSRNTREEIVKLIQSFL"
     gene            3322..3840
                     /locus_tag="PKM34_00020"
     CDS             3322..3840
                     /locus_tag="PKM34_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81993.1"
                     /translation="MKKVSVLFAFLAAFFLTEVSAQTVDGKVTISGFPKGSTLNEATV
                     VDLFKSFRDGKYKINFSYKADNVNRRGVVLFDMKTTVKLDGKIILQSTRGGWPWLPGD
                     MYVPIEAFDLIPGIQKAGIMPTPKLTPDQMDSPLLKGKYEITLEMVSSSEAVKGRIQP
                     LTFSFFGQYPPQ"
     gene            3878..4573
                     /locus_tag="PKM34_00025"
     CDS             3878..4573
                     /locus_tag="PKM34_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81994.1"
                     /translation="MKTFLTLVLFVTLVPARAQKISGKIEGHNKGEMDIVLTMFGFDK
                     LVKIGTLGGSGDFMIDLSANPAGNLSKAEADMYISNLSHGFQFGCGNPDDFPEGEPKI
                     ACDAGFIALWANNEWTGTLFPVSDKDLQPWLEDSGYSDPVQASFYKVLLVTEDVELQK
                     QCNNFDHYNDKDIEIGIEYDLKLKKGLNLVQYKLESIYKTDPNVRASFPTKVKMTDAG
                     ENPEIIWLAKYFY"
     gene            4657..5457
                     /locus_tag="PKM34_00030"
     CDS             4657..5457
                     /locus_tag="PKM34_00030"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNQ81995.1"
                     /translation="MKRMLLVVLMIMGLGIFYAEAQFLKEIRDRAIERSKDVIIGKTA
                     DKAADKTSLAMDKLLNPDLGSLMNRGGSKVDMARLPDFYSFDYRYALKITTAEGDIDF
                     DYFLSKNESYMGAKMNRGMEMSMVFDEGRKVMLTIVGGMATAIEMDLSDAMEEEDMDM
                     YNDYTFKELPNREFLGYDCVGREMENDEYKFTIYIAPDMEAGFGSMFKSEHANIPPAM
                     QKFSSEYENGLMMYMEMVDKKNKKRKNTSATMECVAFEPTDLAINTRQ"
     gene            5486..5842
                     /locus_tag="PKM34_00035"
     CDS             5486..5842
                     /locus_tag="PKM34_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81996.1"
                     /translation="METTEKIVTTTNSTTDEGKTIAIIAYLTIIGFVIALVMNNDKKL
                     PLATFHIRQSLGLMLTSLVVGMIGIIPILGWIINLVAVFFLLYMWIMGLINAVNSREK
                     TLPFLGDKYAEWFNKL"
     gene            5999..7906
                     /locus_tag="PKM34_00040"
     CDS             5999..7906
                     /locus_tag="PKM34_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HNQ81997.1"
                     /translation="MMKTTATLTILLVCLMVTLNLNAQIQRKRTTARPSTTIETVDPS
                     KINQQVTSPNLQNLQIAKGYFTRLKAGTPNQKQGEQELTVVDQNFPAVNTTKVTQVGK
                     TTAQGDICVTENLDIEVNAREFSYISESPESWIRPGQIFTAQSFISGQPATVALARNP
                     INLAIDLRGVTNTVFQVQNPEQNSQLMQAENALISQNATPPAANFSFSFHKIHSVDEM
                     EFKLTGKYRGALGAFSAKFGLNTGNQQEYHYYMLEFQQNMFSIQVDGLTPDRVFKQPG
                     SDMSGYVYIDKVDYGRKGIIVFKSTRTLDELGVNVSAGFNGLLSSASANAAYSQLSKK
                     SEVNVLARFYGGDSPSAIQSMENTVEKGVPDLFTFIRGQSNNHRLAKPVGYTLKNMNN
                     QLLGQNSKRTQTVKTCTPVPLAQIFKLKVTLTDIQCINGRDGGGDNPDDYAIQQYIVY
                     KALGKDMNYVSRDINKFPARQNGPVQVSNVRNILISGDQQNQIHVRQDGDVRQRNRNM
                     ISNSLVFNISLNELNDPNATFKIFTWMKEYSTTTLGSNDDKVLMNNDPISVKIKDVVE
                     ILLGLRNLNANTDFHDTTIGKGVKFHNFGSGFMHLANIQKITPLVLEGPIRVGSPGQK
                     AAVWVQFELLQ"
     gene            8005..11061
                     /locus_tag="PKM34_00045"
     CDS             8005..11061
                     /locus_tag="PKM34_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81998.1"
                     /translation="MTQNFPGSGSSRRDFLKHMGLLGLAAGMPSVLWSCSAGGLKVKS
                     DGIPPYSVWEEMLTALQTSPDHLEGRMNLLIQDGNPENMFRFVRDEIYLMPPSGNSLK
                     GLGKTMRYGIRGVLRYGFATPREKAELLNSMFARANITSSVVYERTDIQPDEVHSFFF
                     RPVERKFEPVIDKKTLNRWSIEMGAEESASEATNPDPGLTESRQLAEKLWEKLPNKDK
                     IRPQSFDFRWDNYRTPAVKFEWEGVTKYAHLFDPEVSFGSLRNMSGGGISTPEAAEYP
                     NERVSISLSYCDGISPENEKVLVQGEWLVHELAGNQVVLSFLHGLDLVRQIITPVGHL
                     RIFTPALSFQSFEADRPYMEALSFLGDPFTLEGKIIDVSGEKAAIGNSTLISKPDEYL
                     QKEIHLMEVKAIPGSYPEVNLLVTPRNAFGEIIEGLSAADFSVSDNDIPVQAIVESNQ
                     RTPRLLILYDTSLSMPAAYSGGNMTAFVETLQNAILDKFPETRITRWETNSDLYTWLI
                     KASHTGNDLVIFATDGDNSDAYDPARMEELKFGPPALVLNVNNSTARHHLASFENMAR
                     ATNGIVIDAKDQLETVVTISGYFEKLEIAPYVFSYRATGRELNHRVSVTADNQRVEAA
                     DNYTFSLIPDDDTIAGEQIVGLYLNVKAGNMAVRRVLSGWDNIGSRNRNPTYADFKDV
                     QNMLMGNILISVEGEGPTLSEALSDILKYRLSTRKWGEAVIQNELKEAVAAHQEGGFV
                     YDENLASLMQPLQSAVTSESLTFAAGPRISVVKTWPGIDRDYSKYSFDYLPTANYTTL
                     ATNPLGAFKITLEKTAQLAILEYRIFDTSTLSVLAQKSMISSREASTSEWFRQLYREG
                     PDAGFWYEKIYRQDGSFKLFDQSASEKAFWKIDERTGELFGLLPDMTGGGTSHIQAQL
                     DELSRVMNMYSLIFGALGVGSLPVGIVATYGLTLVRLYAIATEAIIIMDNTGMDEKIA
                     AALQQLACNVKKEINMATLGPVGSVISGLDNLISLMGGGGIPGMQC"
     gene            complement(11064..11246)
                     /locus_tag="PKM34_00050"
     CDS             complement(11064..11246)
                     /locus_tag="PKM34_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ81999.1"
                     /translation="MWDKGYTLPELGVHAIFVHSFSIDSTMMARTNREGVKVYAEFAT
                     LIGKTSVLKGVYHTRF"
     gene            complement(11274..12443)
                     /locus_tag="PKM34_00055"
     CDS             complement(11274..12443)
                     /locus_tag="PKM34_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82000.1"
                     /translation="MIPPLKISWVAHKGAGPDTGEKATAFLGQFLAPGSFEMTSQPPD
                     IILFMSGGSERMAISLAQAGHPVLLLAIRGNNAYAAATEVMAWMKSNNRYALLSDARD
                     AFESGLVDQWLKVVNVWKNLLGQKAALIGTVSDWLVASEVAAEQLKKVMGVCLEEIPW
                     SELPDYREMITDETLMNRFHGNKVRGLEEAARVLTLLRNEVEKQSLSAIAVECFSLVQ
                     QEKVTACLALAQLNTEGITAACEGDLASMAGMMLVRELTGMVPWMANTTRMEGQTLTL
                     SHCTAAFNLVENVSLPTHYETNTSLAVKGMVKASGVTLFRLSSTLEKAMILTGKVTGH
                     PQHRDACRTQVEIVISPSSADKLKNQPLGNHLLMIPGNWSELLEMVCRYREIIIL"
     gene            12667..14460
                     /locus_tag="PKM34_00060"
     CDS             12667..14460
                     /locus_tag="PKM34_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNQ82001.1"
                     /translation="MKLKYTILTAVLCLAFFKIEAQTGLKMPFHKYAPAPPLGWNSWD
                     IFGTTVTEQQIKEQAEAMAVHLLPSGYRYLTVDIQWYEPESKGHAYQPGAVLAMDAYG
                     RLTPGLTKFPSAAEGKGFKPLADYVHSKGLKFGIHIMRGIPRQAVEKNTPVLGTSVKA
                     QDIALKNSTCPWNPDMYGVDATTPDGQAYYNSIIQMYAGWGVDYIKVDDISRPYDDVQ
                     RAEIEAIRKAIDKTGRPMVLSLSPGATPVKAGDHVMNHANMWRITDDFWDSWGLLHAM
                     FERLDVWTPFRGPGHFPDADMLPIGIIDFNRPTKFTRDEQYTLMSLWAIARSPLIFGG
                     DMTKLDDFTKQMLTHPEMLKVNQESTNNRQVYNVNNLIVWTADVPDSEDKYVALFNAQ
                     SKGDHIDFSHAEYASPVIAGNGSSQEISVSVKGGKNLVLFVNDGGNGFDWDHVAWIDP
                     VLHGPMGDLKLTDLKWINATSGWGNATVDKTCEGRPLIVDGKPATGIGAHAKSVIIYE
                     LPEGYETFTTKGVVLKETGSVVFGVLVDKGKMDFPDRSFVKVDFRTIGMKRKARVVDL
                     WNHTELGVFKREFGRELMQHGAGLYRISPVK"
     gene            complement(14464..16131)
                     /locus_tag="PKM34_00065"
     CDS             complement(14464..16131)
                     /locus_tag="PKM34_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HNQ82002.1"
                     /translation="MNSMKEVKIMLHDYQLFTSGKGNTRVYYKGSASSKDGELLPEGG
                     LPLLPDELENAPRSTELILLEKLSEICGLFSMVIMKEGACYLAVDIIRSSPLFYGFHN
                     NSLFITDNLHEFQKVNDPLPVHEEKLEEFMFTGFVLGSHTIYRDVFATQAGEIVTLEN
                     NQVSSKRYFTYHPLVSTDQQKDDINFPQALDRLLLSAFSRMIRQTPHINRWLVPLSGG
                     HDSRIIVNTLYKLGIRNVVCYTYGTKGNEQSVLSKQVAEALGFEWHFVEYSEQKWDQL
                     HREGVLDNYLHFAFNGVSTPHLQDIPAVYELIHQGVIKNGDVFVPGHSLDLLAGCWLE
                     SGDLIDMGKPGALQRATQKWVGIKEPEFVPSEAIEKVFNSADISPLHFQEYFNWQEFR
                     TKFVVNSIRGYEFFGFESRLPYWDKELVDFWLAIAPQDKIGRKVLFDAEKKGILVNAL
                     TGIPYAGKTPEKSIVKTLLRRLLPERIKNIMLRISGYKVRMNEGLNQIYAMKAHTVKE
                     MLEPLEDFPRHARPYLQKFIDRQPGQVNYNHLTAMYTVRKHMDLWKN"
     gene            complement(16240..18324)
                     /locus_tag="PKM34_00070"
     CDS             complement(16240..18324)
                     /locus_tag="PKM34_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ82003.1"
                     /translation="MGVKNSLALVILVCAGLWLTCCKPAGSPEPHGPVPTENQMRWQE
                     MEFYAFVHFSLNTYTDQSWGYGNEDISLFNPEELDCRQWARICKEAGMKGIIITAKHH
                     SGFCLWPSQYTDYSVKNAPWKDGKGDVVRELADACQEYGLKLGIYVSPWDRNHPDYGN
                     PAYITYFRNQIRELLTNYGPIFEIWFDGANGGSGYYGGANETRQIDRRTYYDWQPTYN
                     MIRELQPGIVIWNDGGDRADVRWVGTEGGWVGETNWSLLNATGDVPFEMLHYGVENGD
                     SWVPGEVNTSIRPEWFYHPSEDSKVKTLPQLMDTYYHSIGRNGTLLLNFPIMPNGRIH
                     PADEKAALDLGQAVKEAFAVNLATHARAEASGVRGNARKFGAQKAIDGNPESFWATDD
                     STTTASMTLDFGKPTRFNRFMAREYIRLGQRVKSFTVEAMTDGEWQEIAKATTIGYKR
                     ILRFPTVEATKLRFHITGAKACPVISEIGVYLAPQILTPPTMIRNQAGDVLITAADQE
                     SEIFYTIDGSDPSPGSMKYSGPIIAGGKLEMKALAYDPTSGKSSTVSHEYFDLPRKDW
                     IIKEIEDEKAYAVLDGNPATAWHQNRGQLPVDLVIDLGHELNLSGFRYHPDKGIWSPG
                     IITEYQFFVSSDQKTWKLVSGGEFSNIKNNPVWQTKRFEEEKARYIRFRALKNAENNN
                     HIGYAEIDLITN"
     gene            complement(18398..19531)
                     /locus_tag="PKM34_00075"
     CDS             complement(18398..19531)
                     /locus_tag="PKM34_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNQ82004.1"
                     /translation="MYRLYRFLPTIAIVLSLAFSSAAQEHKEPATLTIGSKAPDFRLK
                     GTDLKSYTLKSFSKAKILVIIFSAPHCPTAQAYEERIKAIQEDYRGKGVQVVMINPNH
                     AGAVCLEEMGYSESGDTFADMQMRARDKAFNFPFLDDGEEQKTALAYGPVATPHVFIF
                     DQQRILRYCGRIDDREKIGTASQHDTRNALEALLAGKEVPVKETRVFGCSVKWKWKNE
                     YRRKLDEEWAQKQVSLERITLEGIKRLVSNDSKKLRVINFWATWCGPCIAEFSGLVET
                     FRMYQGRDFELVTVSLDKAPLEARVKDFLNLKQAAFTANYLFPGEDKYALIEAVDPQW
                     PGNIPYTLIVEPGGKVIHRFSGGVHMTELRKKIVDHPLIGRYF"
     gene            complement(19555..20514)
                     /locus_tag="PKM34_00080"
     CDS             complement(19555..20514)
                     /locus_tag="PKM34_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNQ82005.1"
                     /translation="MVSCKGPVGSTSPEWSGNPVIQGWYADPEAVIYGDRYWIYPTYS
                     DAYEKQVHFDCFSSPDLVRWTKHERILDTAEVRWARRAMWAPGVMEKGGQYYLFFGAN
                     DVHEGEVGGIGVAVAGKPEGPYRDLIGKPLINNIINGAQPIDQFVFHDRDGSYYLIYG
                     GWQHCNIVRLNDSFTGLVPLPDGSMVKEITPEGYVEGPVMFIREGKYYFMWSEGGWTG
                     PDYRVAYAIADNPFGPFSRIGTILQQDSTVATGAGHHSLLNIPGSDDWVIVYHRRPLG
                     EASPHHRVTCIDRMAFDDQGHILPVKITFEGVTPRPLKTSAGN"
     gene            complement(20868..21773)
                     /locus_tag="PKM34_00085"
     CDS             complement(20868..21773)
                     /locus_tag="PKM34_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ82006.1"
                     /translation="MEKPALFIETYTGITPDTQSKILVSIGIIIVLYVFRTIFLRIVW
                     RNTEDVKTRYLWKRVISYVVPITSIILIGTVWVHAFRYMGAFLGLLSAGIAIALKDLL
                     ANMAGWLFIMFRKPFDIGDRIQIGPHTGDVIDLRLYQFSILEIGNWVHADQSTGRIIH
                     IPNGKVFTEPQANFTQGFHYIWNELEIRLTFESNWQNAKDLLTGIVTRHTQEIIRPAE
                     RDLKEASKLYLIHYQYLTPIIYVTVMEYGVLLSVRYLCDPHKRRGTEAAIWEDILSEF
                     AQHGDIQWAYPTRRFIGDSRGTPAS"
     gene            complement(21855..>22120)
                     /locus_tag="PKM34_00090"
     CDS             complement(21855..>22120)
                     /locus_tag="PKM34_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82007.1"
                     /translation="QNYPNPVSSSTEIGFTLYKTQKVTLTIYDHLGREVTTLVNGLWS
                     PGNHRVTWKISDLPAGKAGNGLYFYRLVTDDIILSRKMMVLGR"
BASE COUNT         5862 a         5054 c         5299 g         5905 t
ORIGIN      
        1 gttctaagct gaagtcatca cttcgtttta gaaatcaact gatttttaac ctattcacta
       61 ttaaaggact gggtccgttt attgtggcgg atcaatgaat gtcaggtaca aatatccatt
      121 caacgagaat ccccttttcc tggattccat gctcaaagcc aggaaaaaat tgaatatgca
      181 tgtggtggtt ttaaagaaaa tcctcaccat catgtattat ctgggtggtg aagcggtaat
      241 cctggtctta tccaacgatt tgggggtcgt aatctcttac tatatttaat tgggaaagtt
      301 taaagggaca ttgataattt tcattctgtc ctttcattta ttggataatt tactgcctgt
      361 gtaaaacaaa gttagcatac tgttgttaat gaagaaagat aattaacatt tgtttgaact
      421 ttcaattgtg agtttcagac aagcgttggt taccaatctg atctcatatg actggcagtt
      481 tttcatttag aattccatac caacggttga cgggaattgc cgggttgctt ttcattttta
      541 ttttgtggta tgtgcaaggg gtatctgcaa agtctgccgg gaatcatttg gatttgggac
      601 tattcaacca gagaattaat gcaaaagcga aagttttcgt tacaactgac ggaaccgttt
      661 ctgcacagaa ggtcttctca gcagcgtttc aaccgaattt taagccattg ccaaaaagat
      721 actttgcctc atccagggca acctattggt accttatgga catcagggct gttgatttgc
      781 ctgacaccgg aaagtggtac gtctgtttta gttactatga tcaaattacc ctctattatc
      841 aaagcggaga ttcactagct acccggactg caggtatgtt taccagaaat aattctgagg
      901 gggtaaatga cctttctgac attccgttta ctcatgacga attgattgac ggccgctatt
      961 tatatgcaca aataaaacat agttcgcgaa aaaaattaat tgtcgatcct tattacacca
     1021 gtgaaaattc aattcaattc tacaaagaat acagcagtcg caagttcttc ctgaatcaaa
     1081 tcccatttta cctgtttact gggggtgtgt tgttgatgtt cttttatttc atcggttttt
     1141 actttatata cagtgacaga cttttcatct attattcgct ttatttgctg gctctgattt
     1201 tatacattgg cgcgagagcg gccttcattc aggaagaatt caggatggct ttgccccatg
     1261 ccatccatat ctataacgat gtgatgcagg tttttgtgaa catcttttat ttgaaattcg
     1321 cacagcaggt tttaaatgcc aaaaccgatt ttccgaaatt aaatatagcc attcgttacg
     1381 ccctgacact cttgattact attgttgcgt ttcagtttgc tatccttgta tttaagcctt
     1441 tttccgccgc cgggcctttt attatagaat ttcaacgcta tttcatgatt gtttttagcc
     1501 tggcatctta tgtttacata attagacatt acaagaaaaa agttgttctg tatttggtcg
     1561 gaggcagcct tttctattta tcgggggcgt tgttagccct ttatctctgg gaaataaaat
     1621 atatgatgct gggtaccagc attgaggtct tcatattttc actcattctg ggttaccgga
     1681 ctaagcttgt cgaacaggag aaaaagtcga ttgaagctga gatgacccag gtaaaactta
     1741 cagccttaaa ggcccagatg aacccccatt ttatttttaa ttccctgaat tcaatcaggg
     1801 cttatgtgat ctccaatgag actaaaaagg catccgatta tttgaacaaa tttgccagac
     1861 tcatccggct catcctctac tattcatcca gggatttcat tctgctgaaa gaggagcttg
     1921 aaacgctcag actttatata gaactggaac agatgcgtta ccgcgatgat tttgggttcg
     1981 agctgaaaat ctccccggct gtggatattg gaaagtgttt ggttccaccg cttattcttc
     2041 agccatatgt tgaaaatgcg atcggacacg gattggctcc caaaaccggt gagaaaaaat
     2101 tgtggctgga aatcgcaaaa aaagaccaca gtgttgtatt tgccattcgc gacaatgggg
     2161 tgggacgacg tttttcaaaa aaggtgaatc atattaaaga ccagaaacat gaatcgatgg
     2221 ctatggaact tacccgaaag cgaattaacc tggcaggtga aaatttaagt gcggataata
     2281 aaattgaaat tattgatatt gaagaatcag gacaatccct gggaaccgaa gtgagattta
     2341 agttacccat tcaaaccaca taagttatag aaatatgaaa ctgaccgccg ctattattga
     2401 tgatgaaatc catgccattg aaacgctgag atatgatttg gatgagaacc accgcaatga
     2461 tgttaaggtt ttgttttcta ccaccaaccc ggtggaaggg gttaaacgca ttaggacaga
     2521 taagcccgat ctgttgtttc tcgatgtgga tatgcccgga ttgacgggtc tggaattgat
     2581 acagttgatc gacgatctcc cggtcaggat catatttacc acagctcaca cagaatacgc
     2641 cataaaggca gttgaaaccg ttgcatcagg ttatctcctt aaacctgttc aaccagatga
     2701 tctgcaacgt gtgattgaaa aggcaatgga tacccttaaa cctgcagcat ttgaaacgcc
     2761 tgttaaagat aaaattcctg tttgcgataa cgatggcatt gaactggtcc cttgtgcaga
     2821 tattatatac tgcaaatcag acagcaacta ctgcaccctg gtcctctacg gaaaaagaag
     2881 aatcacggcc agcaaaaccc tcaaacattt tgaagaactc ctcggtaatg accagtttat
     2941 ccgtgtccac aaatcatttc tgatcaatcg taatcatata aagaaatacc tcaggaaagg
     3001 tggcggggaa ctggtcatgt caaacaacga tgtggttccg ttatcgcgaa atacaagaga
     3061 ggaaattgta aagcttatac aaagcttcct gtaacccccc cttttctgct ttgtgtcagt
     3121 acaaacgcgg gtaccccatg cactgcatac tcattcatac ctgactttcc acatccgaac
     3181 cccgccattc actcattcag ggtcataagg gattcacctt tcatttatct ttatacagga
     3241 tattaaggtt tttatttctc gtatagagag gtggggattc ttcaatctga aaaagagtat
     3301 gaattaacaa attaattgac catgaaaaaa gtttcagttt tattcgcctt cctggcggca
     3361 ttttttctga cagaagtttc agcccagaca gttgacggaa aggtcacgat cagcgggttt
     3421 ccaaaaggaa gtactctaaa tgaagcaacg gtagttgacc tctttaaaag tttcagggac
     3481 ggaaaataca aaatcaattt ttcgtacaag gccgacaatg tcaacagacg cggtgttgtg
     3541 ctgttcgaca tgaaaaccac cgtaaagctg gacgggaaaa taattttgca gagcacacgc
     3601 ggaggctggc cctggctgcc gggagatatg tatgtaccaa tcgaagcatt cgacctgata
     3661 cccggaatac agaaggcggg gattatgcca acaccaaaac tgactcctga ccagatggac
     3721 tcacctcttc tcaaaggtaa gtacgaaata acactggaga tggtatcatc atccgaagct
     3781 gtgaaaggac gcattcagcc gctgaccttc tcattttttg gccagtaccc tcctcaataa
     3841 ttcataacaa acgaacacgg catcaacacc tataaacatg aaaacattcc tgactttggt
     3901 tttatttgtt acgctggtcc ctgcccgtgc gcaaaaaatc agtggcaaaa ttgaagggca
     3961 caataaaggt gaaatggata tagtgctgac catgttcggt ttcgataaac tggttaaaat
     4021 cggaacatta ggcggcagcg gtgattttat gattgacctc tctgccaacc ctgcaggaaa
     4081 tctttctaaa gcagaggctg atatgtatat ttcaaatttg agtcatgggt ttcagtttgg
     4141 ttgcggcaat ccggatgatt tccctgaagg tgagcctaaa atcgcctgcg atgcagggtt
     4201 tattgcctta tgggcaaaca acgaatggac tggaacactg tttcccgttt ccgataaaga
     4261 ccttcagccg tggcttgaag acagtggtta cagcgatccg gttcaggctt cgttctacaa
     4321 ggttttgctt gtgacagaag atgtcgagct tcaaaagcaa tgtaacaatt tcgaccatta
     4381 caacgacaag gatattgaaa taggcattga atacgacctt aaactgaaga aaggattgaa
     4441 cctggtgcag tacaagcttg aatccatcta caaaaccgac ccgaatgtca gggcttcatt
     4501 tcccacgaaa gtaaaaatga ccgatgccgg cgagaacccg gaaataatct ggctggcaaa
     4561 atatttctat taaacccatg aaccggtatc agaggataga actggcaaaa attattatcg
     4621 aagagaaact aaaatgaaat tttaaaagga aggattatga aacggatgtt attggtagtg
     4681 ttaatgatta tgggactggg aattttttat gctgaggctc agttcctgaa agaaattcgt
     4741 gatcgggcaa tcgagcgaag caaggatgtc atcattggaa aaacggcaga taaagctgcc
     4801 gataagacca gcctggccat ggataaactg ctgaatcccg atcttgggag tttgatgaac
     4861 cggggaggaa gtaaggtgga tatggcgcga ttacctgatt tttattcatt cgattatcgc
     4921 tatgccttga aaataaccac tgcggaagga gatattgatt ttgattattt cctcagtaaa
     4981 aacgaatctt atatgggtgc caaaatgaat aggggaatgg aaatgtcaat ggtattcgac
     5041 gaggggagga aagtaatgct gactattgtg ggtggtatgg ctacggccat tgagatggat
     5101 ttaagtgatg ccatggagga ggaggatatg gatatgtata atgattacac ttttaaagaa
     5161 cttccaaacc gtgaattttt gggttatgat tgcgtgggtc gagagatgga aaacgatgag
     5221 tataaattca caatttatat tgctcctgat atggaagcag gattcgggtc catgtttaag
     5281 agtgagcacg ctaatattcc gccagccatg cagaaatttt ccagtgaata tgaaaacggg
     5341 ctgatgatgt acatggaaat ggtggataag aaaaacaaga aaaggaagaa tacaagcgct
     5401 accatggaat gtgttgcttt tgaacctacc gatctggcaa taaacaccag acaataatcc
     5461 attaaaatct actaaaaatt tcattatgga aacaacagag aaaattgtaa caacaacaaa
     5521 ttccaccacc gatgaaggta aaaccattgc cattattgcc tacctgacca ttattggttt
     5581 tgtgatagcc ctagtaatga acaacgacaa aaaactaccc ctggccactt ttcacattcg
     5641 ccagtctttg ggattaatgc ttacatcgct ggtcgtagga atgataggaa taatccccat
     5701 tctgggctgg atcattaacc tggtggccgt ttttttcctg ctctatatgt ggattatggg
     5761 gctcattaat gcagtaaata gccgtgaaaa aacattgcca ttcctcggtg acaaatatgc
     5821 cgaatggttt aataaattat agatcatcct cctgcatcgg ttaccggtga attccccttg
     5881 tgttatttat gccaatgtgg ctatggtttt tagtaagggg aaggggttca ccggagcatg
     5941 cggaactaat taccactgtt tcacgagatg tataataata aagttaaaat ttaaacaaat
     6001 gatgaaaaca acggcaacct taactattct tttagtttgt cttatggtta cccttaacct
     6061 gaatgcacaa atccagcgca aaaggacaac cgcccggccc tcaacaacca ttgaaacggt
     6121 cgatccctct aaaattaacc agcaggttac atcacccaat ctgcaaaacc tgcaaatcgc
     6181 aaaggggtat tttacccggc tgaaagcagg aacacccaat cagaaacagg gtgaacagga
     6241 attaaccgtg gtggatcaga attttccagc cgtcaatacc accaaggtta cccaggtggg
     6301 aaaaactacc gctcaagggg atatttgtgt gacagagaat ctggatatag aagtcaacgc
     6361 cagagaattc agttatattt ctgagagtcc tgaatcatgg atcaggccgg gacagatttt
     6421 tactgcacaa tcgtttatca gcggtcaacc tgctacagtg gcgcttgcaa gaaatcccat
     6481 aaatttggcc atagatttaa gaggggtaac caatactgta ttccaggtac aaaatcccga
     6541 acagaattcg cagcttatgc aggctgaaaa tgccttgatc tcacaaaatg ccactccgcc
     6601 ggcagctaat ttctcgtttt cgtttcacaa aattcactcg gttgacgaaa tggagttcaa
     6661 gttaacggga aaatacaggg gagccttagg tgcattttca gcaaaattcg gactaaatac
     6721 agggaatcag caggaatacc attactacat gcttgaattt caacaaaata tgtttagcat
     6781 tcaggttgat ggcttgactc ctgaccgcgt ttttaagcag ccgggcagcg atatgtcagg
     6841 ctatgtttat attgacaaag ttgattatgg aagaaagggg atcattgttt tcaaatccac
     6901 ccggacttta gatgagttgg gggtcaatgt ttcagcaggg ttcaatggtt tgctaagttc
     6961 agcatcggca aatgccgctt acagccagtt aagtaaaaaa agcgaagtga atgttttggc
     7021 gcggttttat ggcggagatt ctcccagcgc tatccagtct atggaaaaca ctgtagaaaa
     7081 aggggttccg gaccttttca catttatcag ggggcaatcc aacaaccacc ggctggcaaa
     7141 accagtgggc tatactttga agaatatgaa caatcagctg ttaggtcaaa acagtaaacg
     7201 gacacaaact gttaaaacct gtacccctgt gcctcttgcc cagatattta aattaaaagt
     7261 cacactcacc gatattcagt gcattaatgg ccgtgatggg ggaggagata atcctgatga
     7321 ttatgccatt cagcaatata ttgtttacaa agccctggga aaagatatga attatgtaag
     7381 ccgcgacatt aataaatttc ctgcacgaca gaatggccct gttcaggtgt ctaatgtccg
     7441 gaatattttg atttcaggtg accagcaaaa ccaaatccat gtcagacagg atggtgatgt
     7501 aaggcagcgc aatcgcaaca tgataagtaa ctcactggta ttcaacatct cgttgaatga
     7561 actaaatgat ccaaatgcaa ccttcaagat ttttacatgg atgaaggaat acagtaccac
     7621 cactttgggg agcaacgatg ataaagtgtt gatgaataat gatccgattt ctgtaaaaat
     7681 caaggatgtt gtcgaaattc tactgggact aagaaattta aatgccaata ctgattttca
     7741 tgacacaacc attggcaaag gagttaaatt tcacaatttc ggttccggct tcatgcatct
     7801 ggcaaatatt cagaagatta ctcctttggt tttagaaggg cctatccgcg ttggtagtcc
     7861 cggccaaaaa gctgctgttt gggtacagtt tgaattactg caataaacac tgtataattt
     7921 ccgatgagac aaaattgtta tagtgtcagc ggctaaaccc tggctgtatt gatgtatttg
     7981 accacttaag taattcatag gaatatgact cagaattttc ccggatccgg ttcttcgagg
     8041 agggatttcc tcaaacacat gggattgcta ggactggccg ccggaatgcc gtcagttttg
     8101 tggagttgtt cggcaggcgg tttaaaagtc aaaagtgacg gcatcccgcc ttacagtgtg
     8161 tgggaggaga tgctcacggc tttgcaaacg agccccgacc accttgaagg ccggatgaat
     8221 cttctgatac aggatggaaa tcctgaaaac atgttccgtt tcgtgaggga tgagatttac
     8281 ctgatgccgc catccggtaa ttcgctgaaa ggccttggaa aaacaatgcg gtacggcatc
     8341 agaggggtac tgcgttacgg cttcgccact ccccgtgaaa aggcggagtt gctcaattcc
     8401 atgtttgcca gggcaaatat cacttcgtct gtggtttatg agcgcactga tattcagcct
     8461 gacgaagtac actccttttt ttttcgccct gtagaacgaa aatttgaacc ggttatcgac
     8521 aaaaagaccc tgaaccgatg gagcatcgaa atgggggctg aagagagcgc ctctgaagcc
     8581 acaaatcctg accccggttt aacagaaagc aggcagcttg ctgagaaatt atgggaaaag
     8641 cttcccaaca aggacaaaat ccgtccgcaa tcgtttgatt tcagatggga taattaccgg
     8701 acaccggctg ttaaatttga gtgggagggt gtaacaaaat atgcacactt gttcgatccg
     8761 gaagtttctt ttggaagcct gagaaacatg agtggaggcg gtatatctac accggaagct
     8821 gcagaatatc cgaatgaaag ggtcagtatc tccctatctt actgtgatgg tattagtccg
     8881 gagaatgaaa aggtgttggt acaaggtgaa tggctggtcc acgaactggc aggaaaccag
     8941 gtagtgctct cattccttca cggcctggat ttggtaaggc agataattac ccctgtagga
     9001 cacctgagga ttttcacacc ggcattgtcg tttcagtcgt tcgaggcgga caggccttat
     9061 atggaagccc tgtcattttt aggagatccg tttacccttg aagggaaaat aattgatgtt
     9121 agcggagaaa aggctgcaat cgggaattcg acattgattt caaaacctga tgaatacctt
     9181 cagaaagaga ttcatctgat ggaggtaaaa gccattccgg gaagttatcc ggaagtaaac
     9241 ctgctagtaa ctccgcgcaa tgcgtttggt gagataattg aaggcctttc agccgctgat
     9301 ttctcggttt ctgataacga tataccggtt caggccatcg tggaaagcaa ccagcgaacc
     9361 cccagattgc tcattcttta tgatacttct ctgagtatgc cggcagccta ttccggtgga
     9421 aacatgactg cctttgttga aacgcttcaa aacgccatat tggataaatt tcctgagaca
     9481 aggattacca ggtgggaaac taattctgat ttgtatacat ggctaataaa agcttcgcat
     9541 acaggtaacg acctcgtcat ttttgctact gatggtgata atagcgatgc gtatgatcct
     9601 gccaggatgg aagaactgaa attcggacct cctgccctgg ttttaaatgt gaacaattcc
     9661 accgcacggc atcacctggc atcttttgaa aacatggcca gggctaccaa cgggattgta
     9721 atcgatgcaa aggaccagct ggaaaccgtt gtaaccattt ccgggtattt tgagaagctg
     9781 gaaatcgcac cttatgtatt ttcttaccgt gcaacgggaa gggaattaaa ccaccgggta
     9841 tctgttactg cagataatca gcgggtagaa gcagccgaca actatacgtt cagtttaata
     9901 cctgatgatg acactatcgc cggtgagcaa attgtcggat tgtatcttaa tgtaaaggcc
     9961 ggtaacatgg cagtacgaag ggttctgtca ggctgggaca atattggaag ccggaacaga
    10021 aatccaacct acgcagattt taaggatgtg caaaacatgc ttatgggaaa tatcctgatc
    10081 tctgttgaag gcgaaggtcc cacactttca gaagcattaa gtgatatttt aaaataccgg
    10141 ttaagcacac gcaaatgggg tgaggctgta attcaaaatg aattaaaaga agctgtggca
    10201 gcccatcagg aaggtggatt tgtgtacgac gaaaaccttg cgtcattaat gcagccgctc
    10261 caatctgccg taacgtctga aagcctcacc tttgctgccg gcccgcgaat ttctgtcgtt
    10321 aaaacctggc cgggaattga ccgagactat tcaaaataca gtttcgatta tctgcctact
    10381 gcaaattata caacgcttgc aaccaatccg ctcggagcat ttaaaatcac gctcgaaaag
    10441 accgcacaac tggctattct cgagtatcgt atttttgata caagcacact ttcagtatta
    10501 gcccaaaaaa gtatgatttc ttcgcgggaa gccagtacat cagagtggtt caggcagttg
    10561 taccgcgaag gacctgacgc cggtttctgg tatgaaaaaa tatacaggca agatggcagt
    10621 tttaaattgt ttgaccagtc ggccagcgaa aaagctttct ggaaaattga cgaacgaacc
    10681 ggtgaattgt ttggtctgct gcccgatatg accggaggcg gtacaagtca tattcaggca
    10741 cagctcgatg agctaagccg ggttatgaat atgtattcat tgattttcgg ggctttaggt
    10801 gtcggttcat tgcctgttgg tattgttgcg acgtacggat tgacgctggt cagattatac
    10861 gctattgcta cagaggcaat catcattatg gataatactg gcatggatga aaagattgcc
    10921 gctgcattac agcaacttgc atgcaatgtc aaaaaggaga taaacatggc caccctgggg
    10981 cctgttggaa gcgttattag cggcctggat aaccttatta gtctgatggg cggtggtgga
    11041 ataccgggaa tgcaatgctg acttcaaaac cgggtatggt aaaccccttt taagaccgaa
    11101 gttttcccaa tcagggtggc gaattctgca tatactttta ccccttccct atttgtacgc
    11161 gccatcatgg tggaatcgat tgagaagctg tgcacaaaaa tggcatggac acccagttcc
    11221 ggcaaggtgt accctttgtc ccacaaaggt ttcggatgac cgtaaatccc cctttacaat
    11281 attatgattt ccctataacg acacaccatc tctaaaagct ccgaccagtt cccgggtatc
    11341 atcagaaggt ggttgcctaa cggctggttc ttcagtttgt cagctgagga tggggagata
    11401 acgatttcca cctgtgttcg gcaggcatct ctgtgttggg gatggcctgt aacctttccg
    11461 gtgaggatca tggccttttc caatgtggag gagagtctga agagggtcac cccggaagcc
    11521 ttcaccatcc ctttgacagc cagggaagtg ttggtttcat agtgggtggg cagactgaca
    11581 ttttcaacca ggttaaatgc cgccgtacaa tgggaaagcg ttaaagtttg tccctccatc
    11641 cgggtggtat tggccatcca gggaaccatg ccggtcaatt ccctgaccag catcattcct
    11701 gccatggagg ccaggtcgcc ttcgcaggct gcagtaattc cttcggtatt aagttgagcc
    11761 agcgccaggc aggcggtgac tttttcctgc tgaaccaggc tgaagcactc gacggcaatg
    11821 gcgctgaggc tctgtttttc cacctcattc ctcagaagag tcagcaccct ggcggcttcc
    11881 tccagtcccc tgaccttgtt cccatgaaaa cggttcatca gcgtttcatc ggttatcatc
    11941 tccctgtaat ccggaagttc gctccagggg atctcctcca ggcacacccc catgactttc
    12001 ttgagttgtt ctgccgccac ttccgaagcg accagccaat cggagacggt tcctatgagc
    12061 gcagcctttt gacccagcag gttcttccac acatttacca ctttcaacca ttggtcgacg
    12121 aggccgcttt caaaggcatc acgggcatcc gacaacaggg catagcggtt gttggacttc
    12181 atccaggcca tgacctccgt ggcggcggca taggcattat tcccccgtat cgccagcagc
    12241 agaaccggat gaccggcttg agccagggaa atagccattc gttctgatcc tccgctcata
    12301 aacaggatga tgtcgggagg ctggctggtc atctcaaatg aaccgggcgc cagaaactga
    12361 cccaaaaaag ccgtggcctt ttcaccggta tcgggtccgg cacctttatg ggccacccac
    12421 gaaattttca gtggtggaat catgatttgt attttctatg ctcaaaacta agcataaatt
    12481 tgtcgtttat caattgcacc tgattcagga ggtgacatcc ataaatacct gttttggacc
    12541 gttatcccac aaaaatgtgt agtttctatc gcgataaaga ctccgtccgg atttgaaatt
    12601 ttcgtagctt gcacgaactg gtttcgctgg aattttagtt agtttcgaag atttatcata
    12661 aagaccatga agctgaaata taccatttta actgctgtat tatgccttgc tttctttaag
    12721 attgaagcgc aaacgggcct taaaatgcca ttccacaaat atgcgcccgc acctccgctt
    12781 ggttggaaca gctgggatat tttcggtaca actgttaccg aacaacagat taaggaacag
    12841 gccgaagcca tggcagtcca tcttttacca agtgggtata gatacctcac ggttgatata
    12901 cagtggtacg aaccagaatc gaagggtcac gcatatcagc ctggtgccgt tcttgccatg
    12961 gatgcgtatg gccgtttgac gccgggattg acaaagttcc cgtcggcagc cgaaggaaaa
    13021 gggttcaaac cgctggccga ttatgtccat tcaaaaggat tgaaattcgg cattcatatc
    13081 atgcgtggaa tcccgcgaca ggcggtggag aagaataccc cagtgctggg cacaagcgtg
    13141 aaggcacagg atattgcgct taaaaactcc acctgcccct ggaatcccga catgtatggg
    13201 gtggatgcca ccacgcccga tggacaggct tattacaatt caattattca gatgtatgcc
    13261 ggctggggtg tggattacat caaggttgac gatatttccc ggccgtatga cgatgtgcag
    13321 agggctgaga tcgaggccat ccggaaggcg atcgacaaga ccggccgccc gatggtgtta
    13381 agcttgtcgc caggcgccac ccctgtgaaa gcgggtgacc acgtgatgaa tcatgccaat
    13441 atgtggcgca ttaccgatga tttttgggat agctggggtt tgttgcatgc catgttcgaa
    13501 aggctcgatg tctggactcc cttccgtggt cccggccact ttcccgatgc cgacatgctg
    13561 cccatcggaa tcatcgactt caaccggcct acaaaattca cccgcgacga gcagtacacg
    13621 cttatgagcc tttgggcaat tgcccgttca cccctcattt ttgggggtga tatgaccaaa
    13681 cttgacgatt tcaccaaaca gatgcttacc catcccgaaa tgcttaaggt aaatcaggaa
    13741 agcacaaaca atcgtcaggt ctataacgta aacaacctga tcgtatggac tgccgatgtg
    13801 cctgatagtg aagataaata cgtagccctg ttcaacgccc aaagcaaggg cgatcacatc
    13861 gatttcagcc atgccgaata tgccagtccg gtgattgcag ggaatggcag ttcacaggaa
    13921 atcagcgttt cggtgaaagg cgggaaaaat cttgtgcttt tcgtgaacga tggtggaaat
    13981 ggtttcgact gggaccatgt ggcctggatc gatcctgtgt tgcatggccc tatgggtgat
    14041 ttgaaactga ctgatctgaa atggatcaat gccacttcgg gttggggaaa cgcaaccgtg
    14101 gacaaaactt gcgaaggcag gcctttgatc gtggatggaa aacctgccac cggtatcggg
    14161 gcccatgcaa aatctgtcat tatttatgaa ttgccggagg gatacgaaac gtttaccacc
    14221 aaaggagtcg tgctgaagga aacaggttct gtggtatttg gggtgctggt ggacaaaggt
    14281 aagatggatt ttcctgatag gagtttcgtg aaggttgatt tcagaaccat aggcatgaag
    14341 agaaaagcca gggtagttga cctgtggaac cataccgaac tgggtgtgtt caaaagggag
    14401 tttggtcgtg aattgatgca gcatggtgcc ggactttaca ggataagccc tgtgaaatag
    14461 atattagttc ttccaaaggt ccatgtgctt tctgaccgta tacatcgcag tcagatggtt
    14521 gtaattcacc tgacccggtt gcctgtctat gaatttctga agataaggcc ttgcatgcct
    14581 tggaaaatct tcaagcggtt caagcatctc tttgacggta tgggctttca tggcatatat
    14641 ttgattcagc ccctcgttca tcctgacttt atagcctgaa atgcgaagca tgatattttt
    14701 tatcctctca ggcaacaatc ttcgcaacaa ggttttaaca atactttttt ccggtgtttt
    14761 cccggcatag gggattcctg tcaatgcatt gaccaggatc cccttctttt cggcatcaaa
    14821 aagcaccttc cggccaattt tatcctgagg agcaatggcc agccagaaat ccaccaactc
    14881 tttatcccag taaggcaagc gggactcaaa tccaaagaat tcatatcctc tgatgctgtt
    14941 caccacaaat ttggtcctga attcctgcca gttaaaatat tcctgaaaat gaaggggaga
    15001 aatatctgcg ctgttgaaga ccttttcaat ggcctctgag ggtacaaatt ccggttcttt
    15061 aatgcccacc catttctggg tagcccgttg aagcgcaccg ggtttaccca tgtctattaa
    15121 atccccgctt tctaaccagc aacctgccag taagtccagg gaatgcccgg gaacgaagac
    15181 atccccgttt ttgatgactc cctgatggat caactcatat actgccggaa tatcctgaag
    15241 atgcggcgtg gaaacaccgt tgaatgcaaa gtggagatag ttgtccaaaa caccctcacg
    15301 atgcagttgg tcccatttct gttctgaata ttctacaaaa tgccattcaa aacctaacgc
    15361 ctccgcgacc tgtttactta aaaccgattg ttcatttccc tttgtaccat aggtgtagca
    15421 gactacattc ctgattccga gtttgtataa tgtattaaca atgatccggg aatcatgccc
    15481 gccgcttaaa ggaaccagcc agcggttgat gtggggcgtc tgcctgatca tccttgaaaa
    15541 agcagacaat aacagccggt ccagggcctg gggaaaatta atatcatctt tttgctggtc
    15601 agtcgacaca aggggatggt aggtaaagta cctttttgaa cttacctgat tgttctctaa
    15661 ggtaacgatt tctccggcct gagtggcgaa aacatcccgg tatatggtgt ggctccccaa
    15721 tacaaaaccg gtgaacatga attcttccag tttttcttca tggaccggaa gaggatcatt
    15781 gaccttctgg aattcatgca gattgtcggt tatgaaaagt gaattgttgt gaaatccgta
    15841 aaaaagaggg ctgctgcgta tgatatccac ggcaagataa caggccccct ccttcatgat
    15901 taccatggag aacaatccgc agatttcaga gagtttctcc aataggatca gttccgtgct
    15961 tcgtggcgca ttttccaact cgtcaggcag cagaggcagc cctccttccg gtagtaattc
    16021 cccgtctttt gaagaggctg aacctttata atacactctg gtattccctt ttccggaggt
    16081 aaaaagctga taatcatgca gcattatctt tacttccttc attgaattca taaccctgtg
    16141 ttggtccatt ttcaaagaag tctgtacgga tcgcaactga atttacggaa atattccgac
    16201 aaagtcaaat ccggaccgga acagaatgtg gggacggcct cagttcgtaa tcagatcgat
    16261 ttcggcataa ccgatatggt tattattctc cgcatttttc aatgcacgaa acctgatata
    16321 gcgtgctttt tcctcttcaa acctctttgt ttgccatacg ggattattct tgatgttgga
    16381 gaattctccc ccgctgacca gtttccaggt tttttgatcc gaagaaacaa aaaactggta
    16441 ttcagtgata attccggggc tccatattcc cttgtcggga tgataacgga atcctgaaag
    16501 gttcagttca tgtcctaagt cgatcaccag gtcgacgggc aactggcctc tgttttggtg
    16561 ccacgctgtc gcaggatttc catctaggac ggcataggct ttttcatcct caatctcttt
    16621 gatgatccaa tctttacggg gcaggtcgaa gtattcatgg ctcacggtac tgcttttccc
    16681 ggaggtgggg tcataagcca atgccttcat ctccagttta cccccggcaa tgatgggccc
    16741 gctgtatttc attgagcccg gtgaggggtc actgccatca atcgtgtaaa agatttccga
    16801 ttcctgatcg gcagcggtaa tgagaacatc gcctgcctgg tttctgatca tggtgggagg
    16861 cgtgagtatt tgtggcgcaa gatagactcc aatctccgag atcaccggac aggcttttgc
    16921 tccggtgatg tgaaagcgga gctttgttgc ctcgacggtg gggaagcgga ggatacgctt
    16981 gtacccgatg gtggttgctt tggcaatttc ctgccattca ccgtctgtca tggcttcgac
    17041 ggtgaatgat ttcacacgct gccccaaacg gatgtattcc cgggccatga accggttgaa
    17101 gcgggtgggc ttcccaaagt ccagggtcat cgaggcggtg gtggtgctat cgtcggtagc
    17161 ccaaaagctc tctggattgc cgtcgatcgc cttttgtgct ccgaatttcc gggcatttcc
    17221 ccttacaccc gatgcttcgg ccctggcatg ggtagccagg ttgacggcaa atgcctcctt
    17281 tacagcctga ccaaggtcaa gcgctgcctt ctcgtcagcc gggtggatcc tgccgttggg
    17341 cattatggga aagttgagca ataaggtgcc gttccgcccg atggaatggt agtaggtatc
    17401 catcagctgg ggaagggttt tcaccttgga atcctcactg gggtggtaaa accactcggg
    17461 cctgattgag gtgttcacct ctcccggaac ccaggaatct ccgttctcca ccccgtagtg
    17521 caacatctca aacgggacat cgcctgtggc attcagtaag ctccagttgg tttcgccgac
    17581 ccagccgcct tcggtgccca cccagcgcac atcggccctg tcgcctccgt cgttccagat
    17641 gacaatgccg ggttgcaact ccctgatcat attgtaagta ggctgccagt cgtagtaggt
    17701 cctgcggtca atctgtcggg tctcattggc tcccccatag tagccgcttc cgccattggc
    17761 tccgtcgaac cagatctcga aaatgggacc ataattcgtc agcaactccc tgatctggtt
    17821 tctgaagtag gtgatatagg cggggtttcc gtaatcgggg tggttcctgt cccagggtga
    17881 cacataaatt cccagcttca atccatactc ctggcacgcg tcggccagtt cgcgcaccac
    17941 atcgcccttt ccatctttcc agggtgcatt ctttactgaa tagtcagtgt attgagaagg
    18001 ccataaacaa aatccactat ggtgttttgc ggtgatgatg atccctttca tccctgcctc
    18061 cttacagatc cgcgcccatt gacggcaatc cagttcttcg gggttgaaca ggctgatgtc
    18121 ttcatttcca tatccccacg actggtcggt atacgtattc agcgagaaat ggacaaatgc
    18181 atagaactcc atttcctgcc agcgcatctg gttttcagtc ggcacagggc catggggttc
    18241 gggcgatccg gcgggcttgc agcaggtcag ccataaacct gcacacacca ggattacaag
    18301 cgctaaacta ttttttactc ccatttttat tatgttcatt aaatttctgt caggtctttc
    18361 catatggtgt cagatttcgc agctgatagg gtgtacctca gaaatacctc ccgatcaggg
    18421 gatggtccac gattttcttg cgcagttcgg tcatgtgcac tcctccggag aagcggtgga
    18481 tgacctttcc gcctggttcc acgatgaggg tatacggaat attgcccggc cactgcggat
    18541 ctaccgcttc gatcagggca tatttgtctt cacccggaaa gaggtaattg gccgtaaagg
    18601 ccgcctgttt taagttgaga aaatctttta ctctggcttc caggggcgct ttgtcgaggc
    18661 ttacggtcac cagttcgaaa tcacggccct ggtacatcct gaaggtttcc accagtcctg
    18721 agaattcggc gatacagggt ccgcaccagg tggcccagaa attgatgacc cgcagtttct
    18781 ttgaatcgtt actgaccagt cgtttgatcc cttccagggt gatgcgttcc aatgacacct
    18841 gtttttgggc ccattcctca tccagtttcc ggcggtattc gtttttccac ttccacttga
    18901 ccgaacatcc gaagacccgg gtctctttta ccggtacctc tttgccagcc agaagtgctt
    18961 ccagggcatt ccgggtatcg tgttgggatg ccgtgccgat tttttcccgg tcgtcaatgc
    19021 ggccacagta gcgcaaaatc cgctgctggt caaagataaa gacatgagga gtggccactg
    19081 gtccgtaggc cagggcggtt ttctgttcct ccccgtcgtc aaggaacggg aaattaaagg
    19141 ctttgtcgcg ggctctcatc tgcatgtcgg caaaggtgtc gcccgattcg gaatagccca
    19201 tctcctcgag acagaccgcc ccggcatgat tcgggttgat catcaccacc tgcaccccct
    19261 ttccccggta atcctcctga atggccttga tcctttcctc ataggcctgg gcggtcggac
    19321 agtggggggc actgaagatg atcaccagga tttttgcttt ggaaaaggat ttcagggtgt
    19381 aactcttcag atcagtgcct ttcagcctga agtcaggtgc tttgcttccg atagtcagcg
    19441 tagccggttc cttgtgctcc tgggccgcag aagaaaaggc cagggaaaga actatggcga
    19501 tggtcgggag gaatctgtat aacctgtaca tatagaatat tttaaatgaa tgcttcaatt
    19561 cccggcactg gtcttcaatg gccggggggt tacgccttca aaggtgattt taaccggaag
    19621 gatatgtccc tggtcgtcaa acgccatgcg gtcgatacag gtgaccctgt ggtgtgggga
    19681 ggcttccccc agcgggcggc gatggtaaac gataacccaa tcgtcacttc ccggaatgtt
    19741 gagcagagaa tggtgcccgg cgccggtggc cacggtgctg tcttgttgca ggatcgtacc
    19801 gatccggctg aacggcccaa agggattgtc ggcaatggca taggccaccc ggtaatccgg
    19861 accggtccag ccgccttccg accacatgaa gtagtatttc ccctctctga taaacatcac
    19921 cgggccttcg acataccctt ccggggtgat ctccttcacc atggatccgt cgggcaaagg
    19981 tacaagcccg gtgaaactgt cgttcagcct gacgatgttg caatgctgcc accccccgta
    20041 aatcaggtaa taactgccgt cgcggtcgtg gaatacgaac tggtcaatgg gctgggcccc
    20101 gttgatgatg ttgttgatca gtggtttccc gatgaggtcg cggtaggggc cttcgggctt
    20161 gccggctacg gccacgccga taccacccac ctcgccttca tgcacgtcgt tggccccgaa
    20221 gaaaaggtag tactgccctc ctttctccat cactccgggg gcccacatcg cccgccgtgc
    20281 ccaccgcact tccgccgtat ccagaatccg ttcatgtttg gtccatctga ccaggtcggg
    20341 cgaagaaaaa caatcaaaat gaacctgctt ctcataggca tcggaatagg tgggatagat
    20401 ccagtagcgg tccccgtata tcaccgcctc gggatcggcg taccacccct gaatgaccgg
    20461 attccctgac cattccgggg aagtactgcc cacggggccc ttacaggaga ccattgatgc
    20521 cacatataaa aggatggcag gtatggaaaa atatcgaaaa tgcatttttt ctggtatgtg
    20581 ttttaaaacg aatacagacc ttgccgcagg aaaagtaccg catggcgaat atacaccttt
    20641 gcgcccacta tgccaacctt cccgtagctt aatggcttaa gccaggctgt catggcttat
    20701 atccgtgtac aactgtatta tattggcagg aactacccac aaccaaatgg gaaaaattga
    20761 agcttcaggt gatgtgctgc catccagaag ggaccttggt cagaaacggc ttcggtggtg
    20821 aaaccaatga agggtgttgt agtttttgac caaagacggg ggtaggatca ggaggcaggt
    20881 gttccccggg agtcgccgat aaaccgccgg gtcgggtaag cccattggat atcgccatgc
    20941 tgtgcgaatt cgctgagaat atcttcccag atggcggctt cggtacctct gcgtttgtgc
    21001 ggatcgcaca gatagcgcac actcagcagc accccgtatt ccatcaccgt gacatagatg
    21061 atcggcgtca gatactggta gtggatcagg tatagtttgc tggcttcctt gaggtcgcgt
    21121 tcggccggtc tgatgatttc ctgcgtatgg cgggtgacga ttcccgtcag caggtctttg
    21181 gcgttctgcc agttgctttc gaaggtgagc ctgatctcca gttcattcca gatgtagtgg
    21241 aatccctggg tgaagttggc ctgaggttcc gtgaatacct tgccgttggg aatatggatg
    21301 atccttcccg tgctctggtc ggcatgcacc caattgccga tttcgagaat ggaaaactgg
    21361 tagagccgga ggtcgatcac atctccggta tggggcccga tctgaatgcg gtctccgatg
    21421 tcgaacggct tgcggaacat gataaacagc catcccgcca tgttggccag cagatctttc
    21481 agggcgatgg cgatccccgc cgagagaagc cccaggaagg cccccatgta tctgaaggca
    21541 tgcacccaca ccgtcccgat cagaatgatc gacgtgatgg gaaccacgta cgatatcacc
    21601 cgcttccata aataacgggt cttcacgtcc tccgtgtttc tccatacgat gcgcagaaaa
    21661 atagtccgga acacatagag aacaatgatg atccctatgg agaccagaat cttgctctgg
    21721 gtgtccggag tgattcccgt gtatgtctca atgaacaggg ccggtttttc cattttttat
    21781 gcctgatttg aagaagagaa tctttttcag gcgtttaagt tataaaactt aaaggaattc
    21841 ccaactgccg aaagtcacct tcccagcacc atcattttcc tgctcagtat gatgtcgtct
    21901 gtgaccagcc tgtagaagta aagtccgttt ccggcttttc ctgccggcaa atcagagatt
    21961 ttccacgtta ccctgtgatt tcccggtgac cacaagccgt tcaccagggt ggtcacctcc
    22021 cttcccaggt ggtcgtagat ggtgagggtg actttttgcg ttttataaag ggtaaaaccg
    22081 atttcggtgg aggagctgac gggattgggg tagttctgac
//
LOCUS       DAOQXS010000002        18400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_3415_length_18400_cov_3.870373, whole genome shotgun sequence.
ACCESSION   DAOQXS010000002 DAOQXS010000000
VERSION     DAOQXS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_18400_cov_3.870373"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKM34_00095"
     CDS             <1..559
                     /locus_tag="PKM34_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82008.1"
                     /translation="DDNRYGMGMIIGLKEDDITRFRGYGENVEICNRNNPYTYIISGK
                     RDAVEAILSASLEEGALRASLLPVSMPYHSMILHTASTRFGDAIRQMEIKNGKPVYFS
                     ALSQEEIITGDGLRRELIRNIFSRMNWYETMKSLIEKGVTAFYECGAGDGLTKNFRFI
                     GGSFKAYPVDKYRQFIDDPEAISLR"
     gene            complement(552..719)
                     /locus_tag="PKM34_00100"
     CDS             complement(552..719)
                     /locus_tag="PKM34_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82009.1"
                     /translation="MPGTVYYREVKDQIVDHDLSKYNFFNSVMKTKLPPDQFYESVGS
                     LWKIKLGKDVI"
     gene            complement(805..1890)
                     /locus_tag="PKM34_00105"
     CDS             complement(805..1890)
                     /locus_tag="PKM34_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNQ82010.1"
                     /translation="MKILLVNPPRSPENKILEYAPADARPFIHKKLIGPPLGLLTIAA
                     AVKDFDVTVIDLKGEYDLDPDAPPLEQLVLGYLAEYQPDIVGVTFIASELYAGLDIFR
                     TAKKFNPEIITVAGGLHVTICPEDFADPCVDILCPGHSAGIFRNLVIHFDKGLPPDHT
                     AGIILNEHGTLRRTAKPASDWDPAGKDFIHPDRSHLTRWLQTYKVGNDPTPVTYIFTS
                     LGCPFECSFCAIWPQHDKQYYIREVESVIEELKGLDDYGVVRFADANTIVDIRFIDKL
                     FDRILEEGIVKKYVMDIRPDTAARHPELIEKLARAGLKVVICGFESFRDEELDKYNKR
                     SAASNIKKSIEVFHRNDIMIRQLHRSE"
     gene            complement(1907..2554)
                     /locus_tag="PKM34_00110"
     CDS             complement(1907..2554)
                     /locus_tag="PKM34_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82011.1"
                     /translation="MKRASAFLIVLLSGLFIVSCQKPADVTSNPAASGIEKTGNPFVT
                     PEARQALVNTLREVAQEQEAIRGAHFLPAFYTDWGFGFLDDDMVRLASFSTVIDENDF
                     FRENPDGTVTVHIQSETAFSELFNYSTYDYYFGSNGHMVMNYTGPVMMIPIFDNNGNF
                     LGFIYLVQPDNSSPASVWHGNGPVQLWGMGQEYNLVAKLIANAGWKKVNKSIKLN"
     gene            complement(2740..2812)
                     /locus_tag="PKM34_00115"
     tRNA            complement(2740..2812)
                     /locus_tag="PKM34_00115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2777..2779),aa:Gln,seq:ctg)
     gene            complement(2869..6603)
                     /locus_tag="PKM34_00120"
     CDS             complement(2869..6603)
                     /locus_tag="PKM34_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82012.1"
                     /translation="MKAIPALIISLFFIALQISGQVIYIPEDYPTIQEGINAANEGDT
                     VLVAPGTYYENLSLTGENITMGSYFLTTNDTSYISSTIINGGQNGSVITIYQGEDAST
                     RISGFSITNGKGNSGGGIRIFESDPYLDHLNIYGNSASSGGGIYAYWCQPAISNCLIQ
                     GNTATEQGGGIWLGTDSYSWDSPQLTDLVIKDNNAGYGGGIAMDFYIGGYFNGITVCH
                     NTAGTGGGILFGYYGNVVFNPEHRCNIYQNSAAVGNDLYSHANVDVIVDTFTVMNPTE
                     FHASEIEEFTFDILNSKLEQVEYDLYISPEGDNNNSGLTADDPLRTIHHALTILLASE
                     SGQRQIHLLDGIYSPETNGEFFPVSLPDYVHLSGTADSLVILDADSTDRVMQIRDNDT
                     NYVSDLTLCGGLAESGAGIYIYYSDPCLERLIIRDNSSSVGNYSKSGGGISGDHSEAI
                     LKDIIIAGNYAQSSGGGLYFTGSSPVLQNICILNNTAGSGGGLYLSTSTERTICDQLS
                     VLENHASNGAGILNYGFLELRNSIINANVSGNAGGGIYSRDSIWIFNSNLVNNTSGSG
                     GGLYVYANSYALLDSSELSGNHAIGGGGGLCCGTETVVYLNHVKINSNLSNNTAGGIF
                     TATTGLHIKKSDITYNHADNYGGGIYCDDEAFPQFDSTDRCNLFLNWAGTAKDIFTKD
                     GMVNVILDTFTVLHPTDYFVNDPDDFTFDILAGKVEQAYADLYVSTVGSDTNSGFSSD
                     NPLKTIDRALMILRSDSLNQHSIHLVDGIYSGSSNGEKFPLQLSDYCSLEGETRDGTI
                     LDAEDSSHVLNLYHDHIPEIKNLTLTGGHGMNGGGLLGYRSVFNLFNVKIRDNTSNYQ
                     GGGVYVGSGSIAELNHVVFENNESGTGGGIDISYSSAHLKNIDFTHNQATDGGGIYIS
                     YPDTLTMDHIYCFSNTAENYGGAIYARNSDFILANAVFDSNQAYRGGGFCLESSSMDA
                     KNTLFVRNTAADGYGGAGTIYNYGAPPDAEVNFYNATCTQNTPPVLHIYGGKASLTNS
                     ILWDNNNPIQIYYFVDYSESDTLTISYSDIQSAQAGIFKSGGAVLDWNAGNINEDPQF
                     SMGDEYPFSLDAGSPCIDAGTPDTTGLLLPYNDLIGNVRVWDGDADGISIIDMGPYEY
                     GAPVTVDDSSFPEAGKKIHFTLYPNPAKDHLTIQCSDHRGDIIIKLINYSGQIVKVIT
                     LPAGLNMTHIPTDHLGTGAYWVLFTDEEGHSTIEKLIISK"
     gene            complement(6717..8426)
                     /locus_tag="PKM34_00125"
     CDS             complement(6717..8426)
                     /locus_tag="PKM34_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNQ82013.1"
                     /translation="MSTSTPPEDKPGKSLNFIELIIEEDIKNNKNDGRVLTRFPPEPN
                     GYLHIGHAKSICLNFGLGMKYGGKTNLRFDDTNPTKEEQEYVDSIMDNVRWLGFDWEE
                     RLFFASDYFDQLYEWAVMLIKEGKAYVDDHSAEEVSENRGTPTQPGLESPFRNRSVEE
                     NLDLFERMKNGEFPDGTRILRAKIDMASPNMHMRDPVLYRILHAHHHRTGDKWCIYPM
                     YDYAHGQSDYIEGITHSICTLEFEVHRPLYDWFLDQIVKDKNAYRPRQIEFARLDMTY
                     TVMSKRMLLQLVQEGLVNGWDDPRMPTISGLRRRGYTPASIRNFAETIGVAKRENVID
                     VGLLEFCIREDLNKVAPRVMAVLNPLKVVITNYPEGEVEWFDAENNPEDPEAGTRKVP
                     FSREIYIEQDDFMETPPPKYFRLAPGAEVRLKNAYIIKCEEVVKDAGGNITVLNCTYD
                     PETRSHGGSQKKVKGTLHWVSVAQALPVEVRLYDRLFSDPEPAGHEDKDFKEFLNPDS
                     LKVIYPAYIEPNVKGSLPLSHFQFQRIGYFNVDPDTTPERMVFNRTVSLKDTWAKRVN
                     GER"
     gene            complement(8547..8969)
                     /locus_tag="PKM34_00130"
     CDS             complement(8547..8969)
                     /locus_tag="PKM34_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82014.1"
                     /translation="MKPQAENDDLIRNLVRRKGIEKAPDHFTDKVMERINSPSLADDS
                     PLISRGTLLAIIVGVAAMIVVILTVDIPYFDNLFSSDRIQKISMNIFTDGFFRTMASF
                     FREFSSITWVVIGAALGLVLIDRILRKRFSETKGMMIW"
     gene            complement(8981..9544)
                     /locus_tag="PKM34_00135"
     CDS             complement(8981..9544)
                     /locus_tag="PKM34_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ82015.1"
                     /translation="MDDNHYINQVIAGNSALFAVLIDRHKDLVFTLCLNITRSREDAE
                     EVAQDSFLKAFQKLSTFRNDSGFRTWLYRIAYNEAITKVRKKKVVTTSLDDRFIDELP
                     DEEYEESIAGMDEAEQKEVIGRVLEKLPETDRVIVTLYYLESCPVREISEITGLGESN
                     VKVRLHRIRKRIYSELQHIFKIKSLSL"
     gene            9823..10152
                     /locus_tag="PKM34_00140"
     CDS             9823..10152
                     /locus_tag="PKM34_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82016.1"
                     /translation="MLELTPIFVLTIIFGFIYAIIYLNIRRKERMALLEKGADPSIFQ
                     EPKTEKQTSLRYGLFLIGLAIGILMGNVLEVSTELGPEASYFSMVFLFGGIALVISHF
                     LGRKVQS"
     gene            complement(10149..10703)
                     /gene="thpR"
                     /locus_tag="PKM34_00145"
     CDS             complement(10149..10703)
                     /gene="thpR"
                     /locus_tag="PKM34_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNQ82017.1"
                     /translation="MKRLFAAVKIHPSPEFLKTYNSLKAGLRFCRITWVKPESIHVTL
                     KFFGETEEKKIPEISRVLKEAASRHEPFSCELVNVGIFGSSYSPKVIWFGIDQSEPLK
                     KLGTDVLQSVEKIGWEPDRQNFVPHLTIGRIKDVPDKQLFQKMIDMHKTDRLQEFSVS
                     EFNLYESILLKDGPLYKVVESYRL"
     gene            10828..12375
                     /locus_tag="PKM34_00150"
     CDS             10828..12375
                     /locus_tag="PKM34_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ82018.1"
                     /translation="MFYRIGLAFFLISAFFLVRAQDTTYLRYSLTITKEELSAHVYKL
                     ASEEFSGRYTGSKGEYEAGKYILEEFAEDGLQAPVINGKVTYRQVFALDNCRWKDQRL
                     MVNGGELKVGRDFLFLSDPVDIKGNFPVIFAGFGIEDKAYSDFSGLDVKGKILLVFSG
                     EPRDKKGRSVITGKEEQSKKGYYFSKAALATAKGAAGVIIIARKDSDFRDFLKNRDQY
                     ETEPNISYPEPGEEDVTKKEVFSAFVDLKTAAMLVGEKPAVLSAAMAEMESSLATSAG
                     RFAGRVEIDASSDCVALPTANIVAIVEGSEKKHEAVVVVAHYDHLGEFAGDIYYGADD
                     NASGTAAVMELAEAFSRAAAEGVRPKRTVVFLAVSAEELGLYGSRHYTDHPLIPLDST
                     VACINIDMIGRSSEKQVTSDYISGYVYRSPELYALTQHAHRLAAPGLLDRMELRQSVR
                     GGSDHYYFAKHGIPSVFYFTGFHEDYHEPTDTPDKILYTRMEQIVRAIFAVTWKLANG
                     EEKMDSE"
     gene            12523..12596
                     /locus_tag="PKM34_00155"
     tRNA            12523..12596
                     /locus_tag="PKM34_00155"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12557..12559,aa:Ala,seq:ggc)
     gene            12707..13603
                     /locus_tag="PKM34_00160"
     CDS             12707..13603
                     /locus_tag="PKM34_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82019.1"
                     /translation="MKTKALYECIDFLTKWKTIKYDDHIHETYRDAIYGEKYVEPFRR
                     ELNKNLVFLNQTKQNKVIKFYITELIRQIDSEFILSKYKTQDSEESGTLEIKDEAYLK
                     EKEEKYSMADAITYLNMILFFELQMICRSYNIPVKKIIDEANIDLDKYYFIPALKKFK
                     GFEESFTLSIRPRFTAEYIPQIFEFLKDFFDKRDQPEFLTILETGEDAYRPLLFIDKG
                     SRLADVFKQLFSVDIIRGCQKKELEAWICKNFRYRSGKTIKIFTKRYVSDVISSNQVR
                     ITKPILDLKLDKTTGQYLITKL"
     gene            13706..14044
                     /locus_tag="PKM34_00165"
     CDS             13706..14044
                     /locus_tag="PKM34_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ82020.1"
                     /translation="MTEITFEKLPQAVTQIYSKLESIERLLHAQNNLSHPAEDKLLTI
                     REAAEVIHLSVPTIYGLVQRQDIPVCKKGKRLYFSRQELNDWIKSGRKKTVAEITAEA
                     GNYLQIRNKR"
     gene            14051..15241
                     /locus_tag="PKM34_00170"
     CDS             14051..15241
                     /locus_tag="PKM34_00170"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HNQ82021.1"
                     /translation="MKAAKKQPPIVEIQHVLNSWYDLRFNEIRGVVEGRKKDEEDYKE
                     LNENNLFIQLLSNGYKVSITNLCALLNSEYVPTYNPFREYFESLKPWQEGGRDFILEL
                     ANYVEAVDQQQYNHHFKKALVRMIACALEDNTFNKQAFILVGGKQNTGKSTFCRYLCP
                     PALRNYYTETIPGDKDALISLCENFIINLDELSTLSKFELNNLKSMFSKDRVKIRHPF
                     DRKSKSDPRRASFIGSTNEDNFLTDTTGSVRWLCFEIVSINWDYKNIDINQVWSQAYT
                     LFKSGFKYQLTPEEIRLNELRNEQYQQVSMEYEYVQAYLSPGTPFEHDDFLTTTQIRD
                     YILIKTERKAEIKNTDKLGRALKNLGFTRVVKRTGESNSPTRGYYLKYLHEDKMMATC
                     LPPF"
     gene            15417..16271
                     /locus_tag="PKM34_00175"
     CDS             15417..16271
                     /locus_tag="PKM34_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNQ82022.1"
                     /translation="MINKDLSHTSIIDYLELNGHLPVRVIRGNAWYLSPLRDEKTPSF
                     NVNLFKNMWYDFGIGAGGNLYHLMDQLKEFRIPQSRESLWDVRNIEIKRLAWVDDEKS
                     HINILYTRNLQNKSLLEYLKSRRISIFFARKFAREAYYTVHDKKYYAIAFKNDLGGFE
                     LRNAFFKTGSSPKHFTTIPGSVNSKLLVFEGFMDFLSACTYFKRVPQCKCIILNSLSF
                     LPKIEDELARAESISLFFDNDTAGRVATRKIMENHGQVIDRSQTLFSDYKDFNDFLMG
                     KKITQCTE"
     gene            16846..17250
                     /locus_tag="PKM34_00180"
     CDS             16846..17250
                     /locus_tag="PKM34_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82023.1"
                     /translation="MNNEEFVKHIKERENVLLKELEALRTLLELYTNDGKAVQESPAS
                     ISTSNTFRPKGNDTWEDYAVAILEHLDKKSKANEVANIAIAANPKIDKQTVINAIKGK
                     LSKLNIHGTIMSEKGEFQKDGYLYYIKQKEPI"
     gene            complement(17552..18007)
                     /locus_tag="PKM34_00185"
     CDS             complement(17552..18007)
                     /locus_tag="PKM34_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNQ82024.1"
                     /translation="MKTSANLFQICEITVNYSSKVKASNRPKIAGSRDAYELVLPLWD
                     DLEYRESFAILLMNRANRALGIAQVSSGGISGTVADPKVIFQHALKANASCLILLHNH
                     PSGNTQPSEADIHLTKKLVEGGKLLDLAVLDHLIVTTDGYYSFADEGLI"
BASE COUNT         5019 a         4092 c         4403 g         4886 t
ORIGIN      
        1 ggatgataac cgttacggaa tgggtatgat catcggtttg aaagaagatg atattacccg
       61 tttccgggga tatggggaga atgtcgaaat ctgtaacagg aataatccct acacctatat
      121 catctctggc aaacgggatg ccgtggaggc cattttatct gcatcgctcg aagaaggtgc
      181 gctccgggcc agcctgctgc cggtttcgat gccgtatcac tccatgatcc tgcatacggc
      241 atccacccgt ttcggagatg ccatccgcca gatggagata aaaaatggca aaccggttta
      301 tttttcagcg ttaagccagg aagaaattat caccggggat ggcctcagga gggagcttat
      361 ccgcaatatt ttcagccgga tgaattggta tgaaacgatg aaatcactga tagaaaaggg
      421 agtaacggct ttttatgagt gcggcgccgg cgacgggctg acaaagaatt tcaggtttat
      481 cgggggtagt ttcaaggcgt atccggtgga taaataccgg caatttattg atgatcctga
      541 agctatatca ctcagatgac atcttttccc agtttgatct tccataaact tccgacgctt
      601 tcatagaatt gatccggcgg aagttttgtc ttcatcacac tattgaaaaa attgtacttc
      661 gacaaatcgt ggtcgacgat ctggtcttta acctcacgat aataaaccgt tccgggcata
      721 ggtgtgagga tggtataacc ggcataaacg accggatttg aggcggaata agcggccagg
      781 gccttgaaat cttcttcgcc gtaatcattc ggaacgatgt agttgccgga tcatgatgtc
      841 gttgcggtga aagacttcga tcgatttttt aatgttggaa gcggctgagc gcttgttgta
      901 cttatccagt tcttcatcgc ggaacgattc gaatccgcag atgacgactt tcagccccgc
      961 ccgggccagt ttttcgatca gttcgggatg cctggctgcc gtgtcgggac ggatatccat
     1021 gacgtacttt ttcacgatcc cctcttcaag gatgcggtcg aacagtttgt cgatgaaccg
     1081 gatatcgacg atggtgttgg catcggcaaa ccgtaccacg ccatagtcgt cgagtccttt
     1141 cagttcttcg atcaccgatt ccacctcgcg gatgtaatat tgcttgtcgt gctggggcca
     1201 gatggcgcag aaggagcatt caaaagggca tccgagggaa gtgaagatat acgtgacggg
     1261 agtcgggtcg ttgcccacct tataggtttg cagccagcgg gtgaggtggc tccggtcggg
     1321 gtggatgaaa tccttgccgg cgggatccca gtcggaggcc ggtttagcgg tccggcgaag
     1381 cgtaccatgc tcattgagaa tgatccctgc cgtatgatca ggaggtaatc ctttgtcaaa
     1441 gtggatcacc aggttgcgga agatgccggc ggaatgcccc gggcacagga tatccacgca
     1501 aggatcggcg aagtcttccg ggcagatcgt cacatgcaga ccgccggcga cggtgattat
     1561 ctccgggttg aatttttttg cagtccggaa gatatccagg cctgcataga gctccgatgc
     1621 tatgaaggtc actccgacaa tatcgggctg atattcagcc agataaccca gaaccagttg
     1681 ttcaagcggt ggggcatccg gatcgaggtc atattccccc ttcagatcga tgacggttac
     1741 atcgaaatct ttaacggcgg ctgcaatggt caataatccc agcggaggcc cgatcagttt
     1801 cttatgtata aacggccggg catcggcggg agcatactcc aggattttat tctcaggaga
     1861 ccggggtgga ttgacaagga ggatcttcaa accaggagac ccgtcatcaa ttgagtttaa
     1921 tgctcttgtt cacctttttc cagcccgcgt tggcgatcag tttggccacc aggttgtatt
     1981 cctggcccat cccccagagc tgcaccggtc cgttgccgtg ccaaacgctt gccgggctgg
     2041 agttatcagg ctgcaccagg tagatgaacc cgaggaaatt tccgttattg tcgaaaatag
     2101 ggatcatcat gaccgggccg gtgtaattca tcaccatatg gccgttcgaa ccgaaataat
     2161 aatcataggt ggaataattg aacagttcgc tgaaagccgt ttccgactgg atgtggacgg
     2221 tgaccgtacc gtcggggttt tcccggaaga aatcgttttc atcgattacc gtagagaagg
     2281 aggcgagcct gaccatgtca tcatccagga acccgaatcc ccaatccgta tagaaggccg
     2341 ggaggaaatg agcgccacgg atcgcttcct gttcctgtgc aacttcacgg agagtgttga
     2401 caagcgcttg gcgtgcttcc ggcgtgacaa acgggttgcc tgttttttcg attccggacg
     2461 ccgccggatt agaagttacg tcggccggtt tctggcacga tacgataaat agtcctgata
     2521 ataaaacgat taaaaatgcg ctggcccttt tcatagtcgt agttttaggt tatcggccca
     2581 aaaatagatt aaaatatggc tatttatgct ttcgatttct taaatcatta acaatgaaat
     2641 gttaaaagga tgggttggct ggaaagaaaa aaaaattcgc aatacttgtt attgtaattg
     2701 cgaaatgata ttgtgaaatg atattgtgaa atgaaggtgt tgcccgatca ggggtcgaac
     2761 ctgaactctt ctgatccaga gtcagacgtg ttgccagtta caccatcggg caatgagagc
     2821 gcaaaaatag taaattttga atgttcagga tcgaatatag aattattttc atttggaaat
     2881 gatcagtttt tcaatggtag aatggccttc ttcatccgta aacagtaccc aataggcccc
     2941 tgttcccagg tgatctgtcg gaatatgcgt catattcagg ccagccggaa gagttataac
     3001 cttaacaatc tgaccggagt aatttataag cttaatgatt atatctcccc ggtgatcaga
     3061 acattgtatg gtcagatgat ctttggcagg atttggatag agtgtaaaat ggatcttctt
     3121 tccggcttcc gggaatgagg aatcatcaac cgttacaggt gctccgtact cgtacggacc
     3181 catgtcgatg atgcttatcc catctgcatc gccgtcccat accctgacat taccgatcag
     3241 atcattataa ggcaacaaaa gccccgtggt gtcgggtgtt ccggcatcga tgcaaggcga
     3301 tccggcatca agtgaaaagg gatattcatc acccatcgaa aactgcggat cttcattgat
     3361 gttgccggcg ttccagtcga gcacagcccc gcctgactta aagatcccgg cttgggcgct
     3421 ctggatatct gaatacgaaa tggttaaggt atcactttcg ctataatcca caaagtagta
     3481 tatctggatc ggattattgt tatcccagag gatactgttg gtcagactgg ctttgcctcc
     3541 ataaatatgg agaaccggag gtgtattctg cgtacaggtt gcattgtaaa aattcacttc
     3601 agcatctggc ggcgcaccat aattgtaaat cgtaccggct ccgccataac catctgctgc
     3661 cgtatttctg acaaacagcg tgttttttgc atccatcgag gatgattcga ggcaaaaacc
     3721 tcctccccgg taagcctggt tcgaatcaaa gaccgcattg gccaggataa aatctgaatt
     3781 tcgcgcatag atagcaccgc cataattctc tgccgtattc gaaaaacagt aaatatgatc
     3841 catggttaac gtatccggat aagatatgta aatacctccg ccatcagtcg cttgattatg
     3901 ggtaaaatcg atgttcttca aatgagccga ggagtatgaa atatcaattc cgcctcccgt
     3961 tccgctttca ttattctcaa aaaccacatg atttaattca gcgatgctcc cacttccgac
     4021 atatacaccg ccaccctggt agttgcttgt attgtccctg atcttgacgt tgaacaggtt
     4081 aaacacagaa cggtaaccca agaggcctcc tccattcatg ccatgacctc cggtcagagt
     4141 taagttttta atttcaggga tatgatcatg ataaaggtta aggacatgcg atgaatcttc
     4201 tgcatcgaga atggtcccat ctctcgtttc gccttccaga ctgcaataat cggataactg
     4261 caaagggaac ttctccccgt ttgatgatcc gctgtagata ccatctacaa ggtgaatgct
     4321 gtgctgatta aggctgtcag atctcaatat catcagggcg cggtcgatgg tcttcagggg
     4381 gttatcagat gaaaatccgc tgttggtatc acttccgacg gtggatacat aaagatctgc
     4441 ataagcctgt tccacttttc cggcaagaat atcaaaagta aaatcatccg gatcattcac
     4501 aaaatagtcg gtgggatgca agactgtgaa cgtgtcaaga atcacattca ccatcccgtc
     4561 ttttgtaaaa atatcttttg cggtgccggc ccagttcagg aataaattac agcggtctgt
     4621 gctgtcgaat tgtggaaatg cttcgtcatc acaataaatt cctccgccat aattatcagc
     4681 atggttataa gttatatccg attttttgat atgcagaccg gttgtggcag taaagatgcc
     4741 gccggctgtg ttattagata ggttactatt gattttgaca tggttgaggt aaaccactgt
     4801 ttctgtgccg caacacaggc ctcctccgcc cccgatagca tggtttccgg ataattcaga
     4861 tgaatccaga agagcatagg aattggcata cacataaagt ccgcctccgg aaccggatgt
     4921 attattgaca aggttagagt tgaaaatcca gatcgaatct ctggaataaa tcccacctcc
     4981 ggcattaccg gatacattag cattgataat gctgttacga agttcaagga atccataatt
     5041 taaaatcccg gcaccattgg aagcatgatt ttcaaggacg gacaattgat cacatatagt
     5101 tctttcagtg gatgtcgata aatataatcc gccgccgctg ccggcagtat tatttaaaat
     5161 acagatgttc tgcaacactg gcgatgaacc agtaaagtac agaccgccgc cactactttg
     5221 agcataattg ccggcaatga ttatatcttt cagaatggct tccgaatgat caccggatat
     5281 tccccctccg cttttagaat aattaccaac gctggatgaa ttgtcgcgga taataagccg
     5341 ttccaggcaa ggatcagagt aataaatgta aatccccgct ccagattcag ccaaaccgcc
     5401 acacaaagta agatcagaaa catagttggt atcgttatcc cttatttgca tgacgcggtc
     5461 tgtactgtcg gcatccagga tcaccaggct gtcggcggtt cctgaaagat gaacgtaatc
     5521 gggaagagaa acagggaaga actccccgtt ggtttcggga ctatagatgc cgtcgaggag
     5581 atggatctgc cgctggccgg attcggatgc aagaaggatg gtcagggcat gatgaatggt
     5641 tctcagcgga tcatcggcag tcagtccgct gttgttatta tctccctccg gcgagatata
     5701 caggtcatat tcaacctgtt ccagcttgct gttcagtata tcgaaggtaa attcttctat
     5761 ctccgaagca tgaaattccg taggattcat taccgtgaaa gtatcgacga taacatccac
     5821 atttgcatga gaataaagat catttccaac cgcagcagag ttctggtata tgttgcatcg
     5881 gtgttccgga ttgaatacga cattgccata atagccgaac aaaataccac cgccggtgcc
     5941 ggctgtgttg tgacatacgg taattccatt gaaatatcca ccgatataaa aatccatcgc
     6001 aattcctcct ccgtaacccg cattattgtc ctttatgacc aggtcggtca gttgcggaga
     6061 atcccacgaa taggaatccg tccctaacca aatacctcca ccctgctctg tcgcagtatt
     6121 cccctggata aggcagttgg aaatggcagg ctgacaccag tatgcataga taccgcctcc
     6181 gcttgaagcg ctattcccat aaatattcag atgatccagg tatggatccg attcaaagat
     6241 ccttatacct cctccactgt ttccctttcc attggtaatt gaaaagcctg atatcctggt
     6301 agatgcatct tcaccctggt atatggtgat cacagagccg ttctgacctc cattaatgat
     6361 cgttgaagaa atgtaagaag tatcgtttgt agtcaggaaa taggagccca tcgtgatatt
     6421 ttcaccagtc agtgaaagat tctcgtaata tgtccctggc gctaccaaga ccgtatcacc
     6481 ttcatttgca gcattgatcc cttcctggat ggtcggataa tcctcaggga tataaatgac
     6541 ctggccggag atctgcagtg caataaaaaa cagactgatt ataagggcag gaattgcttt
     6601 cattttattg aaaacttgga tcttgtaaag ataatcaaca aaatccaata ttgcgccata
     6661 tcagattaca aagctttagc cacccccctc acgcttcacc gctcaccgtt caccgctcac
     6721 cgttcaccgt tcactctctt cgcccacgta tccttcaacg agaccgtccg gttgaaaacc
     6781 atccgttccg gcgtggtatc cgggtctacg ttgaaatagc cgatccgttg aaattgaaaa
     6841 tgtgaaagtg ggagggatcc ttttacatta ggctcgatat aggcagggta aatgactttc
     6901 aatgaatccg ggttcaggaa ttctttgaag tctttgtctt cgtgtccggc cggctcggga
     6961 tcgctgaaca aacggtcgta gaggcggacc tccaccggta aggcctgggc aacggagacc
     7021 cagtgcagcg tcccctttac tttcttctgg ctgccgccat ggctgcgggt ttcaggatca
     7081 taggtacagt tcagtacggt gatattgccg ccggcgtctt tcaccacctc ttcgcatttg
     7141 atgatgtaag cgtttttcag acggacctcg gccccgggag cgaggcggaa atacttgggc
     7201 ggcggggtct ccatgaaatc gtcctgctcg atgtaaatct cgcggctgaa cggcactttg
     7261 cgtgtcccgg cctccggatc ttcgggattg ttttcggcat cgaaccattc cacctccccc
     7321 tccgggtagt tggtgatgac gactttcagc gggttcagca ccgccattac gcggggcgcc
     7381 accttgttca ggtcctcgcg gatgcagaat tcgagcagtc ccacgtcgat cacgttctcc
     7441 ctcttggcca cgccgattgt ttcggcaaag ttgcgtatgg aggctggggt gtaaccgcgg
     7501 cgcctgagac ccgaaatggt gggcatccgg ggatcgtccc agccattcac aagcccttcc
     7561 tgtaccagtt gcagcagcat ccgcttgctc atcacggtgt aggtcatgtc gagcctcgca
     7621 aactcgatct ggcgtggacg gtaagcattc ttatccttta cgatctggtc gaggaaccag
     7681 tcgtagagcg gcctgtgcac ttcaaattcg agcgtgcaga tggagtgggt gatcccttcg
     7741 atatagtccg actggccgtg ggcgtagtcg tacatcgggt agatgcacca tttgtcgccg
     7801 gtgcggtggt gatgggcatg caggatgcgg tacaacaccg ggtcgcgcat gtgcatgttg
     7861 ggcgaagcca tatcgatctt agccctgaga atgcgtgtgc catccgggaa ttcgccattc
     7921 ttcatccttt caaacaggtc caggttctct tctaccgaac ggttccggaa agggctttct
     7981 aagccgggct gggtaggtgt tccgcggttc tcgctgacct cctcggcgct gtggtcatcc
     8041 acataggctt tgccctcttt gatgagcatg acagcccatt catagagctg gtcgaagtag
     8101 tcggatgcga agaacagccg ttcttcccag tcgaacccga gccagcggac attgtccatg
     8161 atcgagtcga catattcctg ctcttctttg gtcgggttgg tatcgtcgaa gcggaggttg
     8221 gtcttgccgc cgtatttcat gccgaggcca aaattcaggc agatcgattt ggcatggccg
     8281 atatgaaggt agccgttggg ctcgggaggg aaacgggtga ggacgcggcc gtcgtttttg
     8341 ttgttcttta tgtcttcttc aatgatcagc tcaatgaaat tgagtgattt accgggtttg
     8401 tcttcgggag gagtgctggt tgacatggat tcgctgattt tcagtaataa gggtgcaaaa
     8461 ttaaaatatt ttagggcgcg accgggtatt tttcagcgaa aaagcggatt aataggtata
     8521 tgagaaataa agcggattgt cagatctcac cagatcatca tgcctttggt ttccgagaag
     8581 cgcttgcgca ggatccggtc gatcagcacc aatcccagcg ctgcgccgat caccacccag
     8641 gtaatgctcg aaaactcacg gaaaaaagag gccatcgtcc tgaaaaatcc atcagtaaaa
     8701 atattcatcg atattttctg tatcctgtcg gaagagaaga gattatcaaa ataaggtata
     8761 tccaccgtaa gaatcaccac gatcatggcc gcaaccccca cgatgatcgc cagcaacgtc
     8821 ccccgggaga tcaacggcga gtcatccgcc agtgaaggag aattgatcct ctccatcact
     8881 ttgtctgtaa aatgatccgg ggccttttca atgcctttgc ggcgaacaag gttccggatg
     8941 agatcgtcat tttcagcctg gggtttcata tcaacagatt ttatagggat aacgatttga
     9001 ttttaaaaat atgctgcaat tctgaataaa ttctttttct gatccggtgg agcctcactt
     9061 tcacattgct ttcgcccagc ccggtaattt ccgagatctc cctgacaggg caactttcca
     9121 ggtaatacag cgtcacgatc acccggtcgg tttcaggcag cttttccaga acccgtccaa
     9181 taacctcttt ttgctccgct tcatccatcc ctgcaatgct ttcctcgtat tcttcatccg
     9241 gcaattcatc gatgaaccgg tcgtccaggc tggtcgtcac caccttcttc ttcctgactt
     9301 tcgtgatcgc ttcgttgtaa gcgatccggt aaagccatgt ccggaatccc gaatcgttcc
     9361 ggaaggtgct tagcttctga aaggctttca ggaaagaatc ctgtgccacc tcttcagcat
     9421 cttcccggct gcgtgttata ttcaggcaca acgtgaacac aaggtccttg tgcctgtcga
     9481 tcaacacagc gaacaacgcg ctgttgccgg cgattacctg gttgatataa tggttgtcgt
     9541 ccatcgggat taactgttca ttggacggtg aagtgtagta aaggttacaa agttaaatcg
     9601 aaaccaggta aattctttat aggttccgtg aaatctgtgc ccttaatctg tgttaatctg
     9661 tgagaaatct actggttctc acagattaac acagattgaa aacacagaaa ggcacagatt
     9721 taaattgcga tttttaccaa tagtgtaacc tctcccgaat tcatgccgtc aaatgttcaa
     9781 atcgtcaatc aaataaaaat taagaattaa aaatttaaaa ttatgttaga actcactcca
     9841 atcttcgtcc tgaccatcat ctttggtttc atttatgcga tcatttacct gaatatccgc
     9901 aggaaagaac ggatggcact gctggaaaaa ggtgcagatc cttccatctt ccaggaaccc
     9961 aaaacggaaa aacagacatc gctccgctat ggtttattcc tcatcgggct ggccatcgga
    10021 atcctgatgg gcaacgtgct ggaggtcagc accgagctgg gccccgaagc ctcttacttc
    10081 tccatggtct tcttatttgg cggcatcgca ctggtgatca gccacttcct ggggaggaaa
    10141 gttcaaagtt aaagtctgta gctctccaca accttgtaaa gaggcccgtc tttcagcaat
    10201 atgctctcat acaaattaaa ctccgacacg ctgaactcct gcagcctgtc ggttttgtgc
    10261 atgtcaatca tcttctgaaa taattgcttg tcaggcacat cttttattct tcctatggta
    10321 agatgcggaa caaaattctg cctgtcgggc tcccagccta ttttctcgac cgactgtaag
    10381 acgtctgttc ccagtttttt gagtggttcc gactggtcta ttccaaacca gatgacttta
    10441 gggctgtagg agctgccaaa gattccaaca ttcaccagtt cgcagctaaa tggttcgtgc
    10501 cgggaggctg cttctttcaa taccctgctg atctccggta ttttcttctc ctccgtctct
    10561 ccgaagaact tcagcgtaac atggatactt tcgggcttca cccaggtgat ccggcagaat
    10621 ctcaaccccg ctttcagact gttataggtc tttaagaact ccggtgaagg atggatcttt
    10681 acggcggcaa agaggcgttt cataacgtta gcaatttctg gtaaaagtag ttaattgatg
    10741 atcacagttt catttgaaac actttgcatg ggaggggatt aaggggaggg gaaagaataa
    10801 ttaaatttga agaataattg tacgagtatg ttttaccgaa taggattagc tttcttctta
    10861 attagtgctt ttttcctggt tcgagcccaa gataccactt atcttcggta ttccctgaca
    10921 atcaccaagg aagagctttc cgctcacgtt tacaaactgg cttctgaaga attcagcggc
    10981 cggtacaccg ggagcaaagg agagtatgaa gccgggaaat atattttgga ggagtttgcg
    11041 gaggatgggt tgcaggcccc ggtcatcaat ggaaaggtta cgtacaggca ggtgtttgcg
    11101 ctcgataact gccggtggaa ggatcagagg ttgatggtca atggcgggga gctgaaagtc
    11161 gggcgtgatt tccttttttt gagcgatccg gtggatatca aaggcaattt cccggtcatt
    11221 tttgccggat ttggcataga agacaaagcc tattccgatt tcagcggcct ggatgtaaaa
    11281 gggaagatcc tgctggtttt ttccggagag ccgagggata agaagggccg gtcggtcata
    11341 accgggaaag aggagcagtc gaagaaaggg tattatttca gcaaggcggc actggctact
    11401 gcgaagggcg ccgcgggggt catcataata gcccggaagg atagcgattt ccgcgatttc
    11461 ctgaagaacc gggatcagta tgaaacggag ccgaacatct cttacccgga accgggggaa
    11521 gaagacgtta caaagaagga ggtcttttcg gcatttgtcg atctgaaaac cgcggccatg
    11581 ctggtagggg agaagcctgc cgttttatcg gcggcgatgg ccgagatgga atcgtcgctg
    11641 gctacatcgg caggacgctt tgccgggagg gtggagatcg atgcgtcgag cgattgtgtg
    11701 gcattgccga cggccaatat tgtcgccatc gtggaaggat cggagaagaa gcacgaagcc
    11761 gtggtggtgg tggcccatta cgatcacctg ggcgagtttg cgggcgatat ctattacggg
    11821 gccgatgaca atgcttcggg tacggcggct gtgatggagc tggcggaagc cttttcccgg
    11881 gctgctgctg aaggcgttcg cccgaagcgg accgtcgtat tcctggcggt gtcggccgaa
    11941 gagctgggcc tttacggatc gcgccattat acggatcatc cgctgattcc gctcgacagc
    12001 acggtcgcct gcatcaatat cgacatgatc gggcggtcgt cggagaagca agttacgtcc
    12061 gattacattt ccggttatgt ttaccgctcc ccggagctat atgcccttac ccagcatgcc
    12121 caccggctgg cagcgccggg tttgctcgac cggatggagc tgcgccagtc ggtgcgcggc
    12181 ggttcggatc attactattt tgcaaagcac gggatcccgt ctgtttttta cttcaccggg
    12241 ttccacgaag attaccatga acccaccgac acccctgaca agattcttta cacccgcatg
    12301 gagcagatcg tccgtgccat ctttgccgtc acctggaagc tggcgaacgg cgaagagaag
    12361 atggatagtg aatagtgatt agggaataac gaatagtgat tagggaaaaa tggttagtac
    12421 tttaaaaaaa aagtttattt ttgcaccgcg ttttaggatt tcaggttttg aatttgtttg
    12481 gaatttggaa tttggagctt gtgatttgga attttaaaat ttggggtatt agctcagttg
    12541 gctagagtgc ttgactggca gtcaagaggt catcggttcg actccgatat actccacaga
    12601 aaatcaggcg gttacgaaag caaaaagtag ccgcctgatt tatttactaa cattttcact
    12661 aacatttgat gccgggctgg catctgactt taccttaaca tatcgcatga aaacaaaagc
    12721 attgtacgaa tgcatagatt ttttgaccaa atggaagaca atcaaatatg atgatcacat
    12781 acatgaaaca tatagggatg ctatatatgg tgaaaaatat gttgaacctt ttagaaggga
    12841 actgaataaa aaccttgtat tcctgaatca gaccaagcaa aacaaagtaa ttaaatttta
    12901 catcacggaa ttgataaggc aaatcgattc tgaatttata ttaagcaagt ataaaactca
    12961 ggatagtgag gaaagtggta cattggaaat taaagatgaa gcatatctca aggaaaaaga
    13021 agagaaatat tcaatggccg atgcaatcac atacctgaat atgattctat tctttgagct
    13081 tcagatgatt tgcagatctt ataatattcc ggttaaaaaa attatcgacg aggcaaatat
    13141 cgatctcgat aagtattatt ttattccagc ccttaagaag tttaaaggat ttgaggaatc
    13201 ttttacgtta agtataagac caaggtttac tgctgaatat atcccccaga tatttgagtt
    13261 tctaaaggat ttctttgata agcgtgatca accggaattt ctcactatat tggagacagg
    13321 agaagatgcc tatagacctc ttttattcat cgataagggt tcgcgattgg ctgatgtatt
    13381 taaacagctt ttcagcgttg atattataag aggatgccag aaaaaagagc ttgaagcctg
    13441 gatttgtaaa aactttcgtt accgcagcgg taagactatc aaaatattca ctaaaagata
    13501 tgtctccgat gttatctcat caaatcaggt aagaatcaca aaacctattc ttgatttaaa
    13561 actggataaa acaactggac agtatctgat tacaaagcta taaaatctta tacggatttt
    13621 atacgtgtgg gtgcccaccg tattgtaacc ccgctcaaga ccatagtacc attgcagcaa
    13681 gttaaacaat ttaaacttgt aagcaatgac agaaattacc ttcgaaaaat taccccaggc
    13741 cgtcacccag atctatagca agctggaaag tatcgaacgg ctgttacatg ctcagaacaa
    13801 tctatcccat cctgcagagg ataaactgct gaccattcgg gaagctgctg aagtcattca
    13861 tctttcagtt cctacaattt acggactggt tcagcgccag gatataccgg tttgtaaaaa
    13921 aggcaagcgt ctttactttt cccgacagga attgaatgac tggattaaat caggaaggaa
    13981 gaagaccgtg gctgagatca ctgctgaagc cgggaactat ttgcaaatca gaaataagcg
    14041 atagccggat atgaaagccg cgaaaaaaca acctccaatt gttgaaatcc agcatgtact
    14101 gaattcctgg tacgatctac gtttcaatga gatcagggga gtggttgaag gccggaagaa
    14161 ggatgaggag gattacaaag agctgaatga aaacaacctt ttcatccagt tactcagcaa
    14221 cggatataaa gtctcgataa ctaacctctg cgctttgctg aactccgaat acgtccctac
    14281 ctataatcca ttcagggagt atttcgaatc gctgaaaccc tggcaggaag gcggcaggga
    14341 ttttattctt gaactggcca attatgtcga agctgtggac caacagcagt acaaccatca
    14401 ttttaagaaa gctttggtca ggatgattgc ctgcgccctg gaggataaca ccttcaacaa
    14461 gcaggccttt atcctggtcg gagggaagca aaataccggt aaaagcactt tttgccggta
    14521 tctctgcccg ccagccctga gaaactatta cacggaaaca atccctggcg acaaggacgc
    14581 gctgatcagc ctgtgtgaga atttcatcat caacctggat gagctgagca ccctgtcaaa
    14641 gtttgagctt aataacctga aaagcatgtt ttcaaaagac agggtcaaga tccggcatcc
    14701 ttttgaccgt aaatccaagt ccgatcccag gagagcttca tttatcggat ccaccaatga
    14761 ggataatttt ctgacagata ccaccggcag tgtccggtgg ctttgttttg aaattgtcag
    14821 cattaactgg gactataaaa acatcgacat caaccaggta tggagccagg cttatacgct
    14881 tttcaaatcc ggtttcaagt accagctgac ccctgaggag atcaggctga atgaactccg
    14941 gaatgaacag taccagcagg tatctatgga atatgagtat gtacaggctt acctgtctcc
    15001 aggcaccccc tttgagcacg atgatttcct gaccaccacg cagatacggg attatattct
    15061 cataaaaact gagcggaaag ccgagattaa gaatactgat aaactcggca gagcgctcaa
    15121 aaatctggga tttacgcgtg tcgtaaaacg gaccggtgaa tcaaattcac caaccagggg
    15181 atattacttg aaatatctgc atgaggataa aatgatggct acatgtctac cgccttttta
    15241 aatcatagca tttacagggg ttttattgta gccaccttat ttttttttga tgactacaag
    15301 atgactacca gactacaaac aacataacaa ggctgtagtc aggtagtcag ggtagccggt
    15361 ggatacctga acaaacaaga ttttgacact ttacacttaa aaataaatat aacaaaatga
    15421 ttaataaaga tttatcccat acaagcatca tcgactacct tgagttaaat ggacatcttc
    15481 cagttagggt tatacgtgga aatgcttggt atcttagtcc attaagagat gaaaagactc
    15541 caagctttaa tgtcaatctt ttcaaaaaca tgtggtatga tttcgggatc ggagctggtg
    15601 gaaatttgta ccaccttatg gaccaactta aagaattccg gattcctcaa tcaagggaaa
    15661 gtttatggga cgtcaggaac atcgaaataa aaagattagc ttgggttgat gatgagaaaa
    15721 gccacataaa tattttatat accaggaatt tgcagaacaa aagtttgctg gaatacctga
    15781 agtcgagaag aatttctatt ttcttcgcaa ggaaattcgc cagagaagct tattacacgg
    15841 tacatgacaa gaagtactat gccatagcat ttaagaatga tctgggaggc ttcgaattga
    15901 gaaatgcttt ctttaaaacc ggctcaagtc caaaacattt cactacgatc cccggatcgg
    15961 tcaattcaaa gttgcttgtt tttgaaggat tcatggattt cctgtctgct tgcacttatt
    16021 tcaaaagggt ccctcagtgt aaatgcatca tcctgaattc tctttctttt ttgccaaaga
    16081 ttgaagatga acttgccagg gctgaatcaa taagtctttt ttttgacaat gatactgcag
    16141 gcagggtagc aacccggaag atcatggaga accacggaca ggttattgac cggtcgcaga
    16201 ccctattttc tgattacaaa gatttcaatg actttcttat ggggaagaaa attactcaat
    16261 gcactgagta attttactca atacgttgag taaaatacaa agggggtctt ttaacacgag
    16321 atcccttttt tttattttac ccaattattt tttcgttgtg gttccgcgac tggaaagctc
    16381 cctcgtccgc ctgatggcgg actcagtacg cttccccagg tcgctccacc acaactatta
    16441 ttaagaagaa taataaacac tgttgccgca taagcgacat tatattttac ggtggctgcc
    16501 gaaggcagac atgccgtcaa gcgcaggcga agacggcaag taaaatattc agtcgcgttg
    16561 tggcaacttg gcctgcccgg cctgagggca aataagatat ttttaaaggg atcggattgg
    16621 aataaggtat ctttttaagc aaaatgattt tttttgttgt ggttccgcat cagcctcacc
    16681 ccttaagtcc atctttctaa ctcggaagac ggggcatata aacgatataa aggcatgata
    16741 attagttatt taatagtaaa aatatactgt tgaaaacttt ttcccatatt ccatgtattt
    16801 cttcgtgttc tttaacgtat ctttgccatg taaaaaacac acgtcatgaa taatgaagag
    16861 tttgttaaac acataaaaga aagagagaat gtactcttga aagaacttga agcattaagg
    16921 acattgttag agttatatac taatgatggt aaagctgttc aggagtctcc tgcaagtatt
    16981 agtacgagta atacatttag acctaaaggt aatgatacat gggaggatta tgcagttgct
    17041 atccttgaac acttagataa aaaatcaaaa gcaaatgagg tagcgaatat agctattgct
    17101 gcaaatccta aaatagataa gcaaacagtt attaatgcta ttaagggaaa attgtctaaa
    17161 ttaaacattc atggtacgat aatgagtgag aagggagaat ttcaaaaaga cggctattta
    17221 tactatataa aacagaagga accaatttaa ttggttccta aacaagtctg gtaatcatta
    17281 tagccagaaa gacaggacag cttcaacaaa tgagttgagc acttatatga agtctcatca
    17341 gcttctgaag atgctcttac tgtcgggaaa gggggtgtaa cagcactccc tttcttcatt
    17401 tgcaaaggta atagaaattt ttataatgtc aacataaata gatgcacaat cattaatcca
    17461 gtatagaaag aaaattcact ttacccttca cgagcataaa aaaggagagc caggcaccag
    17521 ccgggctctc tgaattttaa gccggtggga atcagatcag cccttcatca gcgaaggaat
    17581 aataaccatc ggtggtgacg atcaggtgat ccagaacagc aagatccagc aatttgcctc
    17641 cctcaacgag cttcttggtg aggtggatat ctgcttcgga gggttgggta tttccggacg
    17701 ggtggttatg aagcaggatc aagcaagaag catttgcttt gagggcatgc tggaagatga
    17761 ctttgggatc agcgacagta ccggaaatgc cgccggagga aacctgagcg atccccaggg
    17821 cacggttagc ccggttcatc agcaggatgg caaaggattc ccggtattcc aggtcgtccc
    17881 agagaggcag cacaagttca taggcgtcac gtgagcctgc aatctttggg cgattggaag
    17941 ccttgacctt ggaggaataa ttgacggtga tttcgcagat ctggaataag ttcgcagatg
    18001 ttttcatgga ttgttgccgt tgccctacgg acttatgttg gcttctggcg cgccgggtta
    18061 gaaatgaaat ttttgcgcaa ccagcaccgg atcggaaatt ctgctgcaag ggttccggtc
    18121 cttttcttta gccgatatca atggccagta acgacaaatg aacccggttc tcgtggagtg
    18181 gataggctgt tgctttcgga gtgaagaaga aaatgacgga acacagcgtg acaaaaggag
    18241 ttaattgggc tggccgattg tcgaagaatt aataacatcg aaattcgaaa atcgacaatc
    18301 gaaaatcagg aggaactcct gccgtccctt gccggcagat atttccgatg taggtaaatt
    18361 cgtggaaaaa tcgagtgact aaaaaggaaa agaggaattg
//
LOCUS       DAOQXS010000003        15810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4141_length_15810_cov_3.905617, whole genome shotgun sequence.
ACCESSION   DAOQXS010000003 DAOQXS010000000
VERSION     DAOQXS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4141_length_15810_cov_3.905617"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKM34_00190"
     CDS             <1..810
                     /locus_tag="PKM34_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNQ82025.1"
                     /translation="IEEREKIKMAEADKSKTEVQKRDKIISDLTKRLDEAQTKLEQGS
                     NKLAGEVKEIELRDFLKATFPIDSIEDVPSGLKGADVLHSVRNSVGQAIGSILYERKQ
                     TAAFSEGWIAKLKEDGRTSKSDVLVLVTQSMPKNNQSTHFRDGVWVSSFSDLLLLITL
                     LRDGLIKQSVALVSQQNKGTKMEMLYDYLLSNDFVNHITGILDAFKKMDHSIQKEKSD
                     AMKRFAERESHVWQAKKSILSFWGRVEGIAADGLNQQMAMLSEGEQDKLEE"
     gene            complement(1595..1669)
                     /locus_tag="PKM34_00195"
     tRNA            complement(1595..1669)
                     /locus_tag="PKM34_00195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1632..1634),aa:Pro,seq:ggg)
     gene            2041..2697
                     /locus_tag="PKM34_00200"
     CDS             2041..2697
                     /locus_tag="PKM34_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82026.1"
                     /translation="MKKIIFVLTLVSLTALNYKGFSQVSERMDSIYRNKEANLPAWTF
                     NFNPVGVALYGPIIQMEFKISNRGYLVPWLRYSYAGVVSTYNFTNFEDDSKYSPMSSA
                     LGIGYKKFIPGDVSRQHIYWGFFGEIMYDECLQNIGSTAGEEFNQTRIAAAAYLNVGY
                     RWKSRRNFYWSLGILPGYVYDLKHEGTFTNSGGPVQQDFKGHKLTGMIDFSMGWNFER
                     "
     gene            complement(2898..3602)
                     /locus_tag="PKM34_00205"
     CDS             complement(2898..3602)
                     /locus_tag="PKM34_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82027.1"
                     /translation="MKKVLLSISIMVIASMAFTQQNQPSPFGNGGKETMNGISYAPTV
                     TCDNEKGYYDFPFTENGITVTGSGTGTYTVYQPGWGSCGIFAAANCVWIGTDGPATYT
                     NTFSSPVNNMIYNMTATDIGEVITITTDAGTPSITYTDGTCPSWFNISGNTITCVDGY
                     YPAAGGRFLITSTQDFSSITFSHNGTMNGTTMTMCFDQAITPPPPSAIPVSNWALILG
                     GALIATFVFVRFRRMI"
     gene            complement(3744..5921)
                     /locus_tag="PKM34_00210"
     CDS             complement(3744..5921)
                     /locus_tag="PKM34_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ82028.1"
                     /translation="MKNCFKVMIISGLACMFSLHAVSQDIWKAINDGDAVKTREWISK
                     DPALLNQRNADLLTPLNLAASLGQKEIAELLLDLGADPAIGDNENSQPIHLAAIYGHV
                     PVVELLLARGNPINTLDDNGMTPLLFAASRGQLDASRFLVEKGADTKIVSSRGINALQ
                     MAVIGGNAELVKYFISKGFSVNSAVRDGLTVLHTAASFGRTEIVKYLVEKGARIDAES
                     EEGAQPLALAVGRNSYDAAQFLISKGVEVGHRDKQGFAAIHEAAGRGNLGVVQLLLDH
                     GADINSLSDAGWTPLTNAAWAPNAAEIGRFLIQHGASVNPDPCREVKSCTCGPNFNTP
                     LHQACAMNKADLVKILIDNGAKVNLLNREGWPPMFSAVRSGNAEVVAYLINHGAFLNV
                     KDPRTGGTELHMAAALGFGEVAGMLIDRGADFKAKNNDGLTPVDFALNYGQNMIAYRM
                     LAAGAADSSLASYFGKECLLAKKYNAGEAEVWFLGHSGWAIKTSSHFLVFDYFDNPRR
                     QKPDNPCLSSGFITDEALAVQNTVVFSSHAHQDHYNRSIFNWDDNNDNVKYVLCHKPA
                     DADNPYTYIPVDESAEVAGMKIQVIRSTDSGGGFLVEVDGLVIFYMGDHSNGNDELMP
                     EFTAEIDKVAQLNKDIDLMFGPIRGCSLGTPEQVKAGTYYALEKLHPALFIPMHSGDY
                     TFEYRNFTEQAHNDGVDTPMKPVFAKGDRFHYLKGEPVAEVYQ"
     gene            6127..6966
                     /locus_tag="PKM34_00215"
     CDS             6127..6966
                     /locus_tag="PKM34_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82029.1"
                     /translation="MKTDDPVRKLRGNKLKRRTLCYNTSGLFNITRILIITILLPCCI
                     PCYYTPNVQNVPLFNSRNQANFTAGYRFGTYSRGWDIQSAYSITDHIGVMGNYQHFGA
                     QYTEWNNGTAVFDNGHFKGDYFEFGAGYYTLIPKEFIFESYAGTGWGKVTNEFSYQFY
                     PVESAVNYNRYFVQPSIGWRHSHIGLAFSTRFCVVHYTRYKMIDPQNTYDINELMGLE
                     HNPCWLLEPAFTFRGGGRIVKFQVQACLSFPLNNKEINYDPFSFSFGVVAMINGKTGR
                     KEK"
     gene            7095..7796
                     /locus_tag="PKM34_00220"
     CDS             7095..7796
                     /locus_tag="PKM34_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82030.1"
                     /translation="MISGCSQNYYVHNTVNVPAFKEKNEAQLAVSYGFSYYESYNLDV
                     QAAHAPFNHLAYMVNYHHYWDDLGENESYQGDFVEFGPGYYKCWADEFVFETYGGVGW
                     GWITNKFELDGENTTSKINYNRYFIQPSVSGRWDGEDWGNMFAKFSIAARFSILDYNT
                     MRLEPENNNYSMSTIIGFASNPTYLIEPGGGLTIGWKYLSVLIQGSIPILLNNYDMKI
                     EDSFHINVGLVGIIH"
     gene            7883..8725
                     /locus_tag="PKM34_00225"
     CDS             7883..8725
                     /locus_tag="PKM34_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNQ82031.1"
                     /translation="MRWQGRRTSGNVEDRRGMSGGKLAVGGGLGTLVIIVIVYLLGGN
                     PGDIMNQLQLGGQEQTTMTTAEEDQLAEFVSVVLADCEDVWTKIFTESGQSYRKPKLV
                     LYTSQVNSACGMAGSASGPFYCPLDEKIYIDLSFMEDMERKLNAPGDFAMAYVIAHEV
                     GHHVQKMLGILGQVQELRGQVSEQEYNEYSVRLELQADFLAGVWAYHAEEMFGILEQG
                     DIQEALGAANAVGDDRIQMQSQGYVVPDSFTHGTSAQRMKWFNKGFNTGDISQGDTFN
                     QENI"
     gene            complement(8765..9157)
                     /locus_tag="PKM34_00230"
     CDS             complement(8765..9157)
                     /locus_tag="PKM34_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82032.1"
                     /translation="MKTFGIILAIIVLVVLVAGTFLYLGIYDEGVRAGTVVRVSKKGV
                     VFKTYEGQLNLQSFGALKNASPFAETFDFSVEKSKPEVVRELEAVALSGERVNLHYVK
                     RYMKFPWRGETKYFITSVEREGERPFEE"
     gene            9401..11479
                     /locus_tag="PKM34_00235"
     CDS             9401..11479
                     /locus_tag="PKM34_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ82033.1"
                     /translation="MKKFLLIPVFLMISFYSAKSSGVCIVDAYQGIYFRLIANHVSVL
                     VNDQIATVVTTQEFRNLTGADHVIKYAFPLHEEASATNIRWKLGGVWYTAVIAPEPQD
                     TTLPGGGNDPDPDLVDHLGETPLYFTLLDTIPDDSTLVIELTYVQLLPYEYSKVDFFF
                     PNNYELIQTFPLNFQSLDFRLYSQRTIEDIQLFSPSGGVIGNYGDSATIFYEITEVAA
                     DFDYSAQYELNSDELGLFAFSTFFPDSMVTCDDYGNGFFTFIVEPDPNDSLNIIQKVF
                     TLIIDRSGSMTGIKMVQAKEAANYIINNLNEGDYFNIVDFETNVSSFQPDHVPFTPEN
                     QQAALDYIYPMYASGSTNISGAFGEAIPDFAGSDSTIANIIIFFTDGQATSGIIGTDQ
                     ILEHIQNLISYNEVSNLSIHTFGIGADVNYSLLSQIASQNNGLCHFLENEELLEAITE
                     FYNMIRNPVLLNPVMTIDPPVIVETYPNPLTNLYIGHQLIITGRYDTAVPVTVTFSGE
                     AYGIQQEYIYNIDLADTVISQNQFLTKLWAKQKMEYLYVLYFTFDEESPEAEEIKNLI
                     IDISICYNVISPFTNYTGGGGTTTVEEDAGTQPDAQRICRFYPNPFSTSGTFNLLVEE
                     DYSGTAFLEIFDNTGKVVMILEIEINGQGNYEIRWDGKDNSGNPLKPGIYLFTVTFGD
                     KSYSGKIFKM"
     gene            complement(11506..13863)
                     /locus_tag="PKM34_00240"
     CDS             complement(11506..13863)
                     /locus_tag="PKM34_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82034.1"
                     /translation="MISSLLNSIEKLKVQILLTTGLLIGVLATGQSSTLTILTPNGGE
                     SYVSGQTIGVTWENTGDPEDLMIEVTEDGGAYWYYMAYVNAGDTSTTLYFQYYGNPTD
                     LAKVRISSYANPSNSDESDSYFTILESPVYFNSPYFGDRYYVNHPVNISWYSYTLTSF
                     NLDFSYDNGATWNSLAANYTGFDFEWDSPSQITDQALIRISDPADPASYGLSPVFSII
                     DEPVAVITQPNGGEIWNYGEYQTVSWDGSNLPPYVNIDFSYDGGLNWTSLGFGYSEPD
                     SGSAQVSVPYYSTENAKVRVTDYSYGFTLDESDATFSVVVPPVIVYYPSGGEYYNGQT
                     TYLNWLALQEISLLNFELSTDNGQTWTLIAEDIPANQGYYYWTVNGTPSQDCLIRMSN
                     STDPSQFGLSGQFTILETPVLTLQEPSAGEIWNTGTPYTISWTYENPAANYIYLEYSV
                     DNGSTWSYIGYVTIDGPQGSFNWTTPDVSSDQCRIRIMDYYLNFVADTSEIFTIITFP
                     ETPICMVSVDSTTNHNVIVWEKPASELIDRFVVYKETSEANVYEIIGTLDYNEQAVLT
                     DTNSNPNVKAYRYRLGFMDAQGHYFPAGADHQTIHLTINQGVGNTWNLIWTGYVGFEA
                     SSYNIHRKTGSGNYEPIGSTSASFTSYTDLTAPSGDVYYVVEVTNPNGCNPARSAEYS
                     SSYSNVATNAVLGVDDPNRELSISTYPNPVKDKLTITTGEMLKGEVRVSISDLLGHTV
                     YSENIQDPGSNGSFTVNTSDLKEGIYMIKISAENGQVTRKIVVNR"
     gene            14078..15367
                     /locus_tag="PKM34_00245"
     CDS             14078..15367
                     /locus_tag="PKM34_00245"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HNQ82035.1"
                     /translation="MTEFQPTRYHLRSLITGNSFPDVNWMLDEPGEGKPSLLQTEYEQ
                     AQLELLGDENGFYKFSGWLPVKKVLQGSSAPVTYKSKDLAVHLGLENLFITFSGWWPE
                     KGAGMRTCSFKETEAYSVCARLDEKNDKVLVVASAGNTARSFARVCSDNNIPLLLCIP
                     EDNLDALWFDTPVNDCVKVICTRSGSDYFDAIHLSALACKLDDFLPEGGAKNVARRDG
                     MATTVLSAVTTIGQIPGYYFQAVGSGTGAIAAWEANKRFQADGRFGKNKMKLIVSQNA
                     PFLPMHDAWKAGSRELLPLDDELARRQVEEIDAKVLSNRKPPYSIHGGLFDALKDTDG
                     DILPVTNEEAKKAGLLFLQKEGVDLHPAAAVATASLIKACQDQTIDKKAVIMLNITGG
                     GEELFRKGRNLFYLKPNALFDINPPIEEVAKELERLF"
     gene            complement(15433..>15810)
                     /locus_tag="PKM34_00250"
     CDS             complement(15433..>15810)
                     /locus_tag="PKM34_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82036.1"
                     /translation="QGFETSDIEKEDFTYSANYIRVPLNFGFKFIGTQTSLAGLKIFL
                     GPSMFIPLSIQENTYPIAKTDVKSPLFDFNVGAGLTIWNVFLDVSYGWGLSPQFKDDP
                     IEAKMQAFYANVGFRFKLINDEE"
BASE COUNT         4277 a         3690 c         3769 g         4074 t
ORIGIN      
        1 attgaggaac gtgaaaaaat caaaatggct gaagcggaca aaagcaagac ggaggtgcag
       61 aagcgagata agatcatcag cgatttaaca aaacggctgg acgaggccca gaccaagctg
      121 gaacaggggt ctaataaact ggcaggggaa gtgaaagaaa ttgagctgcg tgattttttg
      181 aaagccacgt tccccatcga ctccattgaa gacgttccaa gcggattgaa aggcgcggac
      241 gtgctacact cagtccgcaa cagcgttggt caagcaattg gttcaatatt atatgaaagg
      301 aagcaaacag cggctttttc agaaggctgg attgcaaaac ttaaagaaga cggacgaacc
      361 tctaaaagtg atgtattggt gttggttacc caaagtatgc ccaaaaataa ccagagcaca
      421 catttcaggg atggggtgtg ggttagttcc tttagcgatt tactgctgct gatcacgctg
      481 ctgagagatg gcttgattaa gcagtccgtc gctctggtaa gccaacagaa taagggcacc
      541 aagatggaaa tgctttatga ctatttgcta tccaatgatt ttgtcaacca cataacgggc
      601 attctggatg ctttcaagaa aatggatcac agcatacaaa aggaaaaatc cgatgcaatg
      661 aaacggttcg ccgaacgtga aagccacgtg tggcaggcaa agaagagcat actgagtttc
      721 tggggaaggg tggaaggcat tgccgctgac gggttgaacc aacaaatggc tatgctgagc
      781 gagggggagc aggataagtt ggaggaataa ggattagcgg cattatgggc attaaaccgc
      841 ttgattatat agattcttgc gcctatccag acgttgaaat gaccgagtgg tatgaagatt
      901 aatgtaatgc gttctaaagg tatttatcta cgaatcattt cgtattatgt gttattctcg
      961 gtacactttc taatggcacg ataaatatgc ttaaaacttg accttttttt aaatctatgc
     1021 aatttttttt tgaaataatt gaataatgtt catagcatta ataattttgg gcattcaaaa
     1081 gggtgaaagg gtggaatgcc gccactgacg ggggttcccc ctgtatccta cggcgtatct
     1141 ccctgataat caggcatatg tgggggcaga cccgtgtctg tacatttctg aaaaaatctg
     1201 ggttaggaaa aattctggat atttcgacct tatttccatt tcaagcccaa tgccgtcaaa
     1261 aaaattttgg aaaaattaat cccaaaattt ccgacccctc tcttgcttca ttttatctac
     1321 ccgccagatg tttttttggg cttaggttgc acccctgagt tgccctattt tgatttttgg
     1381 gattggggag gagtatgctg gggggatggg aagggatggg cggaaaaaat tttgggggag
     1441 atttagggtg gggatgtttg ggcagattga aaagggtata ataaacaagt caaatacaga
     1501 tatgctatat tataaatata tataaaagtt tgactaatat ttacaatcaa aaaaaataac
     1561 cacctgtatt acaagtggtt attagttcta tttgtcggag tggtgagatt tgaactcacg
     1621 acctcttgac ccccagtcaa gcacgctaac caggctgcgc cacaccccgt aatctaatta
     1681 aaagaacgat ggccactttg gactcacgac cttcccgact tccgtcggga cacgctaacc
     1741 aggctgcgcc acaccccgta atctaattaa aagaacgatg gccactttga actcacgacc
     1801 ttcccgactc agtcgggaca cgctaaccag gctgcgccac accccgcgat gttaaattcc
     1861 gaattccaaa ttccaagcac caaattccaa acaccaaatt ccttacaata actgaaattt
     1921 gaaatcaaaa ggagtgcaaa aatacgaaat ttttatgaaa aacaatttcg tactaatgat
     1981 taatccataa ctttaacggc tcatttgaaa acagccttga actattgttt atcagccaaa
     2041 atgaaaaaaa taatatttgt tctgacactg gtctcactaa ctgccctcaa ctataaaggc
     2101 ttttctcagg tttctgaaag gatggactcg atttaccgca acaaagaagc gaatctcccg
     2161 gcctggacat tcaacttcaa tcctgtcggc gtcgctcttt acgggccgat catccagatg
     2221 gaattcaaga tttccaaccg cgggtatctc gtcccctggc ttaggtacag ctacgccggc
     2281 gtggtcagca cttacaattt caccaatttt gaggacgaca gcaagtacag cccgatgagt
     2341 agcgcgctgg gcataggata caagaagttt attcccggcg atgtgagccg gcagcatatc
     2401 tactggggtt tctttggcga gatcatgtac gatgaatgcc ttcagaatat cggttcgact
     2461 gccggcgaag aattcaatca aacgcggata gccgctgccg cttacctcaa tgtgggctac
     2521 cgctggaaat cgagaaggaa tttttattgg agcctgggca ttctgcccgg ctatgtgtac
     2581 gacctgaaac acgagggaac ctttactaac agcggcggac cggtgcagca agatttcaag
     2641 ggccacaaac tgaccggcat gatcgatttc tcgatgggct ggaacttcga acgataattc
     2701 gatttgattg cttgaaaaaa gctcgtgatc caacgactaa agctgttggt tccgggaata
     2761 ttacaaagat ggaacagact ggacgctcgt cggaataaga acccttgttg aaccgacgga
     2821 ttcaccctcc ggactaccat taaaaaaaaa ggctgtctca ttatctgagc agcctctttt
     2881 ctaaaaacta attatcatta aatcatccta cgaaatctta caaaaacaaa agtggcaatc
     2941 agtgcgccgc ccagaatgag ggcccagttg gatacgggta ttgcagatgg aggaggtggg
     3001 gtgatagcct ggtcgaagca catggtcatg gttgttccat tcatcgtccc attgtgtgaa
     3061 aatgtaatcg atgaaaaatc ctgagtggaa gtaatcagga aacggccacc ggctgcaggg
     3121 taataaccat ccacacacgt aatggtattg ccggagatgt taaaccaact gggacatgtt
     3181 ccatctgtgt aggtgatgga cggtgttcct gcatccgttg tgatggtaat cacttcgcca
     3241 atgtcagtag cagtcatgtt gtaaatcatg ttattaaccg gcgaagaaaa agtattcgta
     3301 taagtagcag gcccatcagt gccgatccag acacaattgg ctgcagcaaa aatcccacac
     3361 gatccccaac ccggttgata gacggtgtaa gtacctgttc ctgaacctgt aaccgtaatc
     3421 ccattttcgg tgaatggaaa atcataatag cccttttcat tgtcacacgt aactgtagga
     3481 gcgtagctga ttccattcat cgtttctttg ccgccatttc caaaaggaga gggttgattc
     3541 tgctgggtaa aagccatcga agcgattacc ataattgaaa tacttagtaa gacttttttc
     3601 atatgtttca gattatgagt ttcaacgtac aaagaaaaaa gattctatct tttcttcaat
     3661 ataaatcgtg tacgaataag cgctttaagc gtccattcgt acacgaaatt tctaaataat
     3721 taaattctaa ctaaacgcag gccttactga tatacttccg cgacgggttc accttttaaa
     3781 taatgaaacc ggtcgccttt ggcaaaaaca ggtttcatcg gagtatcgac accatcgttg
     3841 tgcgcctgtt ccgtaaaatt ccgatattcg aatgtataat cgcccgaatg catcggaatg
     3901 aacaaagcgg gatgcagttt ttcgagggca taataggtcc ccgccttaac ctgttcgggt
     3961 gttccgaggc tgcacccacg gatcggcccg aacatcaggt cgatatcctt gttcagttgc
     4021 gccactttgt cgatctctgc cgtaaattcc ggcatcagct catcgtttcc attactgtga
     4081 tctcccatat agaaaatgac cagcccgtcg acctccacga gaaaaccacc tcccgagtcg
     4141 gtcgaacgga tcacctggat tttcataccg gcaacttcag cgctctcatc gaccgggatg
     4201 tatgtataag gattatccgc atcggctggt ttgtggcaaa ggacatattt cacgttatcg
     4261 ttgttgtcgt cccagttgaa gatagaacga ttgtaatggt cctggtgggc atgggagctg
     4321 aaaaccaccg tattctgcac tgcaagcgct tcatccgtaa taaaacccga tgacaggcag
     4381 ggattatccg gtttctggcg gcggggatta tcgaaataat cgaataccag gaaatgactg
     4441 ctggttttaa tggcccagcc actgtggccc aggaaccaca cctccgcttc gccggcattg
     4501 tattttttag ccagtaaaca ctctttgccg aagtaagaag ccaggctgct gtcagcggcg
     4561 ccggcagcca gcatccggta ggcaatcatg ttctggccgt aattcagggc aaaatcgacc
     4621 ggcgtcaggc catcattgtt tttcgctttg aaatcggcgc cccggtctat cagcatgccg
     4681 gctacctcgc caaagcccaa ggctgcagcc atatgcaatt cggtgcctcc cgtacgggga
     4741 tccttaacat tcaggaacgc accgtgattg atcaggtaag cgacgacctc agcatttccg
     4801 ctcctgacgg cagaaaacat tggtggccaa ccctcacggt tcagcaggtt tactttggcg
     4861 ccattatcta tcagtatctt caccaggtcg gctttattca tggcacaagc ctgatgcagc
     4921 ggggtgttga agttcggccc gcaggtgcat gatttgactt cccggcatgg atcggggttg
     4981 acggaagccc catgctggat caggaagcgg ccaatttcag cggcgttggg cgcccaggct
     5041 gcattggtga gcggagtcca gccggcatca gacaggctgt tgatatcggc gccatgatca
     5101 aggaggagct gcaccacacc caggtttccc cttccggcgg cttcgtgaat ggccgcaaag
     5161 ccctgcttgt cgcgatgacc gacctccaca ccctttgaaa tcaggaattg ggcggcatcg
     5221 taagaattcc ggccgacagc caatgccagc ggctgtgcgc cttcctccga ctccgcatca
     5281 atcctggccc ctttctctac caggtatttg acaatttcgg tccgtccgaa cgaagcggcc
     5341 gtgtgcagta ccgtcaggcc gtcgcggaca gcggaattga cagagaaccc tttgctgata
     5401 aaatatttaa ccagctctgc atttcccccg atcaccgcca tctgaagcgc gttgatcccc
     5461 cgggaactca ctattttcgt gtcggcccct ttttctacca ggaagcggga agcatccagt
     5521 tgcccccggc tggcggcaaa caacagcggc gtcatcccgt tatcatccag ggtattgatt
     5581 ggattgcccc gggccaggag cagttcaacg acaggaacat ggccataaat tgcggccagg
     5641 tgaataggct gcgaattctc attatcacca atggccggat cggcgccgag atccaggagc
     5701 aattcagcga tttccttttg ccccagtgat gcggccaggt tcaacggcgt cagcaggtcg
     5761 gcattgcgct ggttgagaag tgccggatct ttagaaatcc attcgcgggt ttttacggca
     5821 tcaccgtcat taatggcttt ccagatgtct tgcgacacgg catgaaggga gaacatgcag
     5881 gctaaacctg aaatgatcat tactttaaaa cagtttttca taagtacttt ttctttagga
     5941 gaggtgaatt tgcgttcaat ttatacccgg taaacagatt tttaacgacc agatttacag
     6001 aatgatcgga tcggtttgca attggcgtcc cgggatgaag aatcgaattg acaacgttta
     6061 aatttattcg tatctttacg gtaattgcca tttaacaaac gcgttaaacc tttaaacgca
     6121 gatccgatga aaaccgatga tcctgtcagg aagctcagag gtaataaatt gaaacggcga
     6181 acgttgtgct acaatacctc aggtcttttc aacattaccc ggatattaat tatcacgatt
     6241 ttactgcctt gctgcatacc gtgttattac actccgaatg tgcagaacgt ccctttgttc
     6301 aacagccgga accaggcaaa tttcacagca ggttaccggt ttgggaccta ctcgaggggg
     6361 tgggatattc agtcggctta ttcgatcacc gatcacatcg gggttatggg caattatcag
     6421 catttcgggg cacaatatac ggaatggaac aacggcacgg ctgtttttga taacggtcac
     6481 ttcaaagggg attattttga attcggggca ggttattata cactcattcc caaagaattc
     6541 atattcgaat cgtatgccgg aaccggctgg ggaaaagtca ccaacgaatt cagttatcaa
     6601 ttttacccgg tagagtcggc cgtcaattac aaccggtatt ttgtccagcc ttcgatcgga
     6661 tggcgtcatt cccatatcgg gctggccttt tccacccgct tctgcgtagt acattacacc
     6721 cgttacaaaa tgattgatcc gcaaaacaca tacgacatta atgaactaat gggtctcgaa
     6781 cacaatccct gctggttgct ggagcctgct tttaccttca ggggcggcgg aaggatcgtc
     6841 aaattccagg tacaggcctg cttgtcgttt ccattgaaca ataaagagat caactatgat
     6901 cctttcagtt ttagtttcgg ggtggtggca atgatcaacg gtaagacggg gaggaaagaa
     6961 aaataagtca gtatgaatgc caattacatt aaagcatcaa tcattactag ttttatatta
     7021 atctaaaaca caatcatttt atgagtgatt atcataagtc tgttcggaaa tcttgctgga
     7081 tcatattaag tatcatgatt tccggttgtt cgcagaatta ttatgtgcac aataccgtca
     7141 atgtacccgc tttcaaagaa aagaacgaag cacaactggc tgtatcttat ggattctcct
     7201 attacgaatc atataatctt gacgtgcagg cggcacatgc tccctttaat catcttgcct
     7261 atatggtcaa ctaccaccat tactgggacg atttggggga gaatgaatcc tatcagggag
     7321 actttgttga gttcggacct ggttattata aatgctgggc agatgaattt gtttttgaga
     7381 cttacggcgg agtcggatgg ggatggataa ccaataaatt cgaattagat ggtgaaaaca
     7441 caacttcaaa aataaattat aaccggtatt ttatccaacc ttcagtcagt ggccgatggg
     7501 atggtgaaga ctggggaaat atgttcgcaa aattctcaat cgctgcaaga ttctccatcc
     7561 tggattacaa tacgatgcgg cttgagccag aaaacaataa ttactcaatg tcgacaatta
     7621 taggttttgc tagtaatccg acctacctga ttgaacccgg cggtggatta acgataggat
     7681 ggaagtatct gagcgttctg atccagggtt ccataccaat tctgcttaat aattatgata
     7741 tgaaaataga agattcattt cacatcaatg tcgggttggt tgggatcatt cattaacaga
     7801 agctaaaagt tagggacgcc atccgccagc cggcggatgg cgtcctcttt tttttattac
     7861 ctttatctca aaaccaagtt atatgcgctg gcaaggaaga agaacaagcg gaaacgtgga
     7921 agaccgccgc gggatgagcg gtggcaaact cgccgtcggt ggcggcctgg gaaccctggt
     7981 gatcatcgtg atcgtctatc tgctgggcgg taatccggga gatatcatga accaactgca
     8041 actgggcggg caggaacaaa ccaccatgac aacagccgaa gaagatcaac tggcggaatt
     8101 cgtttcggtg gtgctggccg attgtgaaga tgtctggacc aaaatcttta cggaatcggg
     8161 gcaatcgtac cggaaaccca agctggtgct gtacacttca caggtcaatt cggcctgtgg
     8221 catggccgga tcagcctccg ggccttttta ctgcccgctg gatgaaaaga tctatatcga
     8281 cctgagcttt atggaagata tggagcggaa actgaatgcc ccgggcgatt ttgccatggc
     8341 ctatgtcatc gcgcacgagg tcgggcatca tgtacagaaa atgctgggca tactgggaca
     8401 ggtccaggaa ctgagaggac aggtcagtga acaggaatac aacgaatatt cggtacgact
     8461 ggagctccag gccgacttcc ttgcaggcgt ctgggcgtat catgcggagg aaatgttcgg
     8521 aattcttgag caaggcgata tccaggaggc cctgggcgct gccaatgccg tcggcgacga
     8581 ccggatccag atgcaatcgc agggctatgt cgtccccgac tcatttaccc acggcacctc
     8641 tgcccaacgg atgaaatggt tcaacaaagg gtttaacacc ggcgatatca gtcagggaga
     8701 cacttttaac caggaaaata tctgatccaa accttattca accttcaaca ttcaaccttc
     8761 aacattattc ctcaaacggc ctctcccctt ccctttccac actggtgata aaatatttcg
     8821 tttcgcccct ccagggaaat ttcatgtagc gcttgacata atgcaggttc acccgttccc
     8881 cgctcagggc cacggcttcc agttccctca ccacttccgg cttgcttttc tctactgaaa
     8941 aatcaaacgt ctctgcaaac ggagaggcat ttttcaacgc accgaacgat tgcaggttca
     9001 actgaccttc gtaggtcttg aacacgaccc ctttcttgct taccctgacc acggtgccgg
     9061 ccctgacgcc ttcatcgtag atgcccagat acagaaaagt gcctgctacc agcacgacga
     9121 gaacaatgat ggccagaatt attccgaaag ttttcatgaa gtccgatttt gtgatactca
     9181 ttccggttaa caaaggtaat tcaaatatct cgtaaaagcc agaatggcaa acaggcaccg
     9241 ggaaactaca ggaattcaat aaaaatatta accggatgca actgtttttc catgagaact
     9301 gtcttattat cgatgggttc aaggttgaat aacaaacagg gttgatccgg gctgattgaa
     9361 taatttgctg aatattaaag ccattccaaa taccctcaat atgaaaaagt tcttactaat
     9421 cccggttttt ttaatgattt ccttctactc cgccaaatcc agcggcgttt gcattgtgga
     9481 tgcataccag ggaatttatt tcaggctgat cgccaaccat gtaagcgttt tggtgaatga
     9541 ccagatagct acagtggtca cgacgcagga attcaggaac ctcacaggtg ccgatcatgt
     9601 tataaagtat gcttttccgc ttcacgaaga ggccagcgcg accaatatcc gctggaaact
     9661 gggcggggtc tggtataccg ccgttatcgc accggaaccc caggacacca cgctgccggg
     9721 cggaggaaat gacccggacc ccgacctggt ggatcatctc ggagaaacac ctctctattt
     9781 tactttactg gataccatcc cggatgactc aacgctggtc attgaactca cctatgtcca
     9841 gctgctgccc tatgaataca gtaaggtgga tttctttttc cccaataatt atgaacttat
     9901 ccagactttt ccgctgaatt ttcaatcgct ggatttcaga ctttattctc aacgaacgat
     9961 tgaagacata cagctatttt ctccttccgg aggtgtaatc gggaactacg gcgacagtgc
    10021 cacaattttc tacgagatta cggaagtggc cgccgatttc gattattcgg cgcaatacga
    10081 actgaactcc gacgaattag gacttttcgc tttcagcacc tttttccccg attccatggt
    10141 gacctgcgac gactacggaa acggtttctt caccttcatc gtggagcccg atccgaatga
    10201 cagcctgaat attattcaaa aggtgtttac cctgatcatc gaccggtcgg gaagtatgac
    10261 aggtattaaa atggtgcagg caaaagaggc ggccaattac attattaaca acctcaatga
    10321 gggcgattac ttcaatatcg tggacttcga aacgaacgtc agctctttcc agcctgacca
    10381 tgttcccttt acacctgaga accagcaggc tgcgctggat tatatctatc cgatgtacgc
    10441 cagcggttca acaaatattt caggcgcttt tggcgaggca atacccgatt ttgccggaag
    10501 tgattcaacg attgccaaca tcatcatctt ttttacggat ggccaggcta cctccggtat
    10561 tataggaaca gatcaaatcc tggaacatat ccagaacctt atttcatata acgaggtgag
    10621 caatctttct attcatactt tcggaattgg tgcggatgtc aattactcat tactctctca
    10681 aattgcttcg caaaataacg gcctttgcca ttttcttgaa aatgaagaac tactcgaagc
    10741 cattaccgaa ttctacaaca tgatccggaa tccggtactg ctcaacccgg tgatgaccat
    10801 cgatccgccc gtcatcgtgg aaacttatcc gaatcccctg acgaatcttt atatcggcca
    10861 ccagcttatc attaccggaa ggtacgatac tgctgttccc gttaccgtaa cattcagcgg
    10921 cgaagcgtat ggtattcagc aggaatatat ttacaacatc gaccttgccg atacggtgat
    10981 cagccagaac caattcctga caaagctctg ggctaagcaa aagatggagt acctctatgt
    11041 cctctatttc acctttgatg aggaaagtcc ggaagcagag gaaattaaaa atctgatcat
    11101 cgatatcagc atttgctata acgtgatcag tccgttcacc aactataccg gaggaggcgg
    11161 aacgaccacc gtagaagaag atgccggaac ccagccggat gcccaacgca tctgccggtt
    11221 ctacccgaat ccattttcca cgtcaggcac tttcaacctg ttggtggaag aggattacag
    11281 cggaactgcc ttcctggaaa tcttcgacaa cacgggaaaa gtcgtcatga tcctggagat
    11341 tgagatcaac ggtcagggta attatgagat caggtgggat ggaaaggata attccgggaa
    11401 tcctttgaaa cccggaatat acctctttac agttactttc ggcgataaat cttatagcgg
    11461 gaagattttt aagatgtaga aagaattgaa tttaccggca aagatttatc tgttaacgac
    11521 aatctttcgg gtcacctgcc cattttcggc actgatcttt atcatataaa tcccttcttt
    11581 caagtcagat gtattgactg tgaaggaacc gttcgatccg ggatcctgaa tattttccga
    11641 atagaccgta tgccccagaa gatcactgat agaaaccctg acctcgcctt tcagcatttc
    11701 acccgtcgtg atggtcagtt tatccttgac cgggtttgga taggtggaga tgctcagctc
    11761 cctgttcgga tcatccacac ccagtacggc attggtggcc acattggagt aagagctgct
    11821 gtattctgcg ctcctggccg gattgcagcc gttggggttg gtcacttcca caacatagta
    11881 gacatcgcct gaaggggcgg tcaggtcggt gtagctggta aagctggccg atgtgcttcc
    11941 gatcggttca taattgcccg aaccggtttt ccggtggata ttgtaagatg atgcctcaaa
    12001 tcccacatag ccggtccaga tcaggttcca ggtattgcca actccctggt tgatcgtcag
    12061 atggatagtc tggtgatcgg cgccggccgg gaaataatga ccctgggcat ccatgaatcc
    12121 cagtcggtaa cgataagctt tcacattcgg attgctgttg gtgtcggtaa gaacggcttg
    12181 ttcattgtaa tccagggtgc cgataatttc atatacattc gcttccgatg tttccttgta
    12241 taccacgaac cggtcgatca gttccgaggc aggcttttcc cagacaatga cattgtggtt
    12301 agtggtgctg tcgaccgaaa ccatacagat tggggtttcc gggaaagtga tgatggtaaa
    12361 gatttcgctg gtatcggcca caaaattcag gtagtagtcc atgatcctga tccggcactg
    12421 gtcagacgac acatccggag ttgtccagtt aaagctgcct tgcggtccgt cgatagtgac
    12481 ataaccgatg taactccagg ttgatccgtt atcgactgag tattccaggt agatgtaatt
    12541 cgcggcagga ttctcatagg tccatgaaat agtgtagggg gttccggtat tccagatctc
    12601 gccggcagac ggttcctgta gcgtgagtac cggggtttcc aggatcgtaa attgcccgct
    12661 gagaccaaat tgggaaggat cggtggagtt gctcatccgg atgaggcagt cctgggatgg
    12721 cgtcccgttg acggtccagt aataataacc ctggttggcg gggatatctt ctgcaatgag
    12781 tgtccaggtc tgtccattat ctgtcgataa ttcgaaattc aacaagctga tctcctgcaa
    12841 ggccagccag ttgagatagg tcgtctggcc gttatagtat tcaccgccgg aagggtagta
    12901 aacgatgacg ggaggaacaa ccacagaaaa tgtcgcatcg ctctcatcca gggtaaaccc
    12961 atatgaataa tcggtgaccc ggactttggc attctcagtg gaataatatg ggacgctgac
    13021 ttgtgccgat ccgctatccg gttcactata gccgaagcct aagctggtcc agttcaagcc
    13081 gccatcatac gaaaaatcga tattaacgta aggagggaga ttgcttccgt cccaggaaac
    13141 cgtctggtat tcaccgtagt tccatatttc tcccccgtta ggttgagtga taaccgccac
    13201 cggttcatct ataatagaaa aaacagggct caatccgtaa ctggccggat cggccggatc
    13261 agaaatgcgg atcagtgcct gatccgttat ttgtgacggc gagtcccatt cgaaatcaaa
    13321 tccggtataa ttagccgcca ggctgttcca ggtggccccg ttatcgtatg aaaaatccag
    13381 gttgaatgag gtcagcgtgt aggaatacca ggaaatattt acagggtggt tcacataata
    13441 cctgtcgccg aaataaggtg aattaaaata taccggtgat tccagaatgg taaaatagct
    13501 atcgctctca tccgaattgg aaggattggc atagctggag attcttacct tggccaaatc
    13561 ggttggattg ccataatact ggaaataaag ggtggtagaa gtatcgcctg catttacata
    13621 agccatataa taccaatacg cccctccgtc ttcggtaact tctatcatca ggtcttccgg
    13681 atcgcctgtg ttttcccagg tcacaccgat agtctggccg gatacatagg attctcctcc
    13741 gttgggcgtg aggatagtca gggttgagct ttgaccggtt gctaatacac cgatcagcaa
    13801 gccggtggtc agaaggatct gcaccttcag tttttcgatt gagtttagta gagaagagat
    13861 catgatgagt aatttttaat tcttaatttt tagtttttta ttcttaatta tttctttatt
    13921 gtaaatgatt tgattttagt tgatggagca aaataacagt atgaatacgg gtattgaaaa
    13981 tttaaaacga tcagccggga aaactgttca ctgaacggga atttttcagc cccgggtgaa
    14041 tttcatacct ttgcccggaa ttaatgatga tgacccgatg actgaatttc aacccacccg
    14101 ataccatctc cggtcgctta ttacgggcaa ttcattcccc gatgtgaact ggatgctgga
    14161 cgaacccggt gaaggaaaac cttcgctgct gcaaaccgaa tatgaacagg ctcagctgga
    14221 attgctgggt gatgaaaatg ggttctacaa gttttccggg tggctgccgg tcaaaaaggt
    14281 tctgcagggt tcctctgccc ctgtcactta taaaagcaaa gacctggctg tgcacctcgg
    14341 gctggaaaac ctttttatca cattcagcgg ctggtggccg gagaagggtg ccggcatgcg
    14401 tacctgttca tttaaggaaa cagaggccta ttctgtttgt gcaaggctgg atgaaaagaa
    14461 tgataaagta cttgtggttg catccgccgg caatacggcc cggtcgttcg cccgcgtctg
    14521 ctccgacaac aatatccctc tcctgctctg catccccgag gataacctgg atgccctctg
    14581 gtttgacaca cccgtaaatg actgtgtaaa agtgatctgc acccgttcgg gcagcgatta
    14641 cttcgatgcc atccacctgt cggccctggc ttgtaagctg gatgatttct taccggaagg
    14701 gggagcaaaa aatgtggcac gaagggatgg aatggcaacg acagtccttt cggccgtaac
    14761 gacgatcggg cagatacccg gctactattt ccaggctgtg ggcagcggca ccggggctat
    14821 tgcagcctgg gaggctaata aacgcttcca ggccgatggc cgtttcggca aaaacaagat
    14881 gaaactgatc gtatcccaga atgcgccttt cctgccgatg cacgatgcct ggaaagccgg
    14941 ctcgagagaa ctgctcccgc tggatgacga actggcccgc cggcaggtgg aagaaatcga
    15001 tgcaaaagtg ttgtcgaacc gcaagccgcc gtattctatc cacggcggat tgttcgatgc
    15061 cctcaaggat acggatggcg atatcttgcc tgtgaccaat gaagaagcta aaaaagccgg
    15121 cctattgttc ctgcagaaag aaggggtcga tctccatccg gctgccgctg tggcaacagc
    15181 atcgctcatc aaagcctgcc aggatcagac catcgataaa aaagccgtca ttatgttgaa
    15241 tatcaccggt ggtggagaag aattgttcag aaaaggtaga aatttatttt acctgaagcc
    15301 caatgccctg ttcgacatca accctccaat tgaagaagtc gcaaaagaat tggaaaggtt
    15361 gttctgaaat tgaatactaa agacattgaa attcgaactt tgaactttga actttgaact
    15421 ttgaactaaa ttctattctt cgtcattaat caatttgaaa cggaaaccca cattggcata
    15481 gaaagcctgc atcttggctt cgatcgggtc atccttgaat tgagggctca gtccccatcc
    15541 gtagctcaca tccaggaaaa cattccagat ggtcagacct gcgccgacat taaaatcaaa
    15601 caaagggctc ttaacatccg ttttggcgat cgggtaagta ttttcctgga tactcagagg
    15661 aataaacata gagggaccaa ggaaaatttt caatccggcc aggctggttt gggtgccgat
    15721 gaatttaaaa ccgaaattca gcgggacgcg aatataattg gcactatagg taaaatcctc
    15781 tttctcaata tctgaggttt cgaagccctg
//
LOCUS       DAOQXS010000004        15703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4191_length_15703_cov_4.171843, whole genome shotgun sequence.
ACCESSION   DAOQXS010000004 DAOQXS010000000
VERSION     DAOQXS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_15703_cov_4.171843"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKM34_00255"
     CDS             complement(<1..844)
                     /locus_tag="PKM34_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNQ82037.1"
                     /translation="MMLSEEIKARIFSPGNDQDWNCLALEIFRYQAEENPVYRSFLTY
                     LNVNPDVISHYTDIPFMPVGFFKDHVVVTGDSKSAEKVFTSSSTTGTVPSRHFICDLR
                     LYEESFLKAFRLFYGEVANYRFLALLPGYLERTGSSLVYMLDFLIRMTERNGSGFFLH
                     DHDALEARLLQPVEGTIKTILFGASYALLDFSERHPIDLSHAIIMETGGMKGRRREMV
                     REELHHLLCQRFQSKSIHSEYGMTELLSQAYSQGNGVYRAPPWMKVQVRDPNDPLTLL
                     PAGKT"
     gene            931..1455
                     /locus_tag="PKM34_00260"
     CDS             931..1455
                     /locus_tag="PKM34_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82038.1"
                     /translation="MALGFLTAIVSCQRTEEYPVEPRITFLEFEKILNETDSVFDKGV
                     LKFKFTDGDGDIGLSEGDTLPPFNPGSKYYYNLLIDYYEVRNGAETYVPLTSYNSETG
                     LYDTVYLSARVPLLTPEGSNKAISGEIHDSLFIYNYNSDFDTLFLTFRLVDRALNESN
                     TERTPYIVRSFSPQ"
     gene            1462..3291
                     /gene="mrdA"
                     /locus_tag="PKM34_00265"
     CDS             1462..3291
                     /gene="mrdA"
                     /locus_tag="PKM34_00265"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNQ82039.1"
                     /translation="MRKTLESRKYVFLTLFVIAGAVFTIRLFLLQVVQEKYKLSADNN
                     VLRYVTQYPPRGVVFDRNGKLMVYNEAAYDLLVIPKQVKAFDTTEFCRLLEIPIEEVR
                     LRLKKARSYSMVKTTVFLEQLSKEDFSALDEVLFKFPGFFVQLRTVRKYDTPTAAHVL
                     GYTGEVNNRDIERDPYYKMGDYIGLTGLEKSYETILRGKKGMKIKLVDVHNREMGSYQ
                     DGKYDSAAVAGKNITVSIDAEVQEYAEKLMRFKRGSIVAIEPSTGEILVMVSSPSYDP
                     NLLVGRGRSKNFAEFNKDTLKPLFNRAIQAQYPPGSTFKTVNTLIGLQEGVLHDYTRY
                     GCSGTSTTPIPCSHSHPSPLDLYHAIEQSCNPYFWQVFRSILDQRKFRTMQDSYNYWR
                     EEVMSFGIATILEGDVVDQKSGNLPKDTYYDRYYGKTGWKPLTIRSLAIGQGEVEQTP
                     LQLANVAAIIANRGFYHPPHLLKAVEGDTEPAKKFEKKIPARIASTYYETLIEGMSMV
                     YNGGGGSARYYKIDSVECCGKTGTSQNPHGKNHSVFIAFAPRENPKIAIACVVENSGY
                     GATWAAPIASLVMEKYLKRTIKQTATEERMLNAYLIEGSDPGD"
     gene            3275..4537
                     /gene="rodA"
                     /locus_tag="PKM34_00270"
     CDS             3275..4537
                     /gene="rodA"
                     /locus_tag="PKM34_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNQ82040.1"
                     /translation="MTPATKNIYGKIDWTTVWLYIILVLMGWVSVYSSVYDETHSSIL
                     DISQRYGKQMIWIIAGGVIAFVIMLTDAKVFNAFAYIIYGISILILIAVLLIGTEISG
                     SKSWFQIGSVAIQPAEFAKFATCLALARYLSSYATDMNVLQTKIVATALIFTPALLIF
                     LQNDTGSAVVYIALILVLYREGLSGYFLISGVIMVVLFVMTLLINQYIMTGILSVLAV
                     LLYYYNKLLRKRWWIIIGIWLASVAIVFAVDYVFDNFLEEHQKTRINVLLGKESDPKG
                     AGYNVNQSKIAIGSGGLIGKGFLQGTQTKYNFVPEQSTDFIFCTVGEEWGFLGSTAVI
                     GLFVYLLFRLVRMAERQRSDFSRIYGYCVISILFFHFVVNIGMTIGLVPVIGIPLPFF
                     SYGGSSLWAFTILLFIFIKLDASRLALF"
     gene            complement(4534..6657)
                     /locus_tag="PKM34_00275"
     CDS             complement(4534..6657)
                     /locus_tag="PKM34_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNQ82041.1"
                     /translation="MAIIGTLRKHSALAVILVGVAIAAFIVSDLFSGKRGRGNGVPAV
                     GTIAGEEITVTDYNRRVDVNLENQKANQNKESLTPGETFDIRQSTWNQYLDEIIMGNE
                     YKKLGLSVTTEELYDLVQGPRPHNLIQQYFKDPQTGMYSPTVVQNFLQNLDNLTPEVR
                     KQWLDLEKYIKEDRLDQKYKSLVTKGYYMPQAFAALDFESKKKNAEVRYVVARYTVIN
                     DDEVTLTDKDYETYYEKNKQSYEQEPSRDIDYLVFDVAPSAKDREDARQEAYRIYDEF
                     RNASDFVTFVNSTSDKRYDSTWYKKGLLPLNIEVALWDAPAGTFVPPYEENNAWHMAR
                     LMDIQARPDSMKAEHILIAFQGALRAGENVTRTKTQAEKTADSIFTIVSADKTKLQAL
                     AYLLSDDGSAKSNNGDLGWFSDGAMIGPFNDAVLNGKVGDVVKVETAFGYHVVKITGK
                     KDPEQKIRVAVIERTIAPSSKTFQDVYAQASTFAGENSTQAKFDAAVSSQGLNKRSAT
                     YLREMGNTIAGIDYPREIVRWAFFDGIEVGDVSPVFDIGGAYVVAVVTAAREKGIMPL
                     EQMKENIKSFVMNEKKASMLKDRIKGIGTDIYQIAREFNTKVDTNLTLTFSSRNIPGF
                     GSEYQVIGEIFSMEAGQQSEPIQGNGGVFVVVVDRFYEPPQPADYNANRDQLLAAFRS
                     RMGANPMFNALQKITKIEDNRLLYF"
     gene            complement(6813..7403)
                     /gene="lptC"
                     /locus_tag="PKM34_00280"
     CDS             complement(6813..7403)
                     /gene="lptC"
                     /locus_tag="PKM34_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNQ82042.1"
                     /translation="MKTRPSLGENKILLIIIAGIIALMGPAIFSSCKNDVQTVLSLDF
                     VDTLPEMTARDIEILYSEKGQMQIKLVSPYLVNKKGDEELLLFPEGFTVFFYDTAMNL
                     TTKITADYGISYEKKKLMEARHNVVVENLEKEEKLNTEELFWDRAKAVIYSNQFVRLT
                     TGGNVVTGTGLTSKEPFDVLDITHVQGTLEIKDEPQ"
     gene            complement(7415..8995)
                     /locus_tag="PKM34_00285"
     CDS             complement(7415..8995)
                     /locus_tag="PKM34_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82043.1"
                     /translation="MKRLFFVITFSLIFAGIPSVNSFGQEDQGAGKYGSDSLTCITNI
                     SLYREFFKQWKASDYKSETIHDLIPPWRWVFLNCPRGTQNTYIDGAKILSYLIESTND
                     SALKYKENKETFNYFDKLKNRYVDTLMMLYDQRIKYFGKEGFVLGRQGVDLFNYRPQD
                     IENIFNYLHRSIELEGDNTLGAILPYYMDAAINLAKERSDSSLIFDAYESSMQIVENN
                     LKKLASESITKNLKVGLNDSETRVYLGVPKSVNKSVTSNGETEQWIYDNLYLYFENGI
                     LTSWQEQNISPDKGNWGLIKASIDFKLEPFATCPDLVTIYRKKFNQTPDDVELLKKIS
                     NLLEQKKCHTDPFYFETTVKLYELEPSPESAYMIGKMLLNEGKYAEAIDYLKQAEKLN
                     DTATVQKSYMYIAKAYQALNNYPSSRAYALKAAALDPTDGDPYMLIGDLYAASARDCG
                     GNDLTNHVAYWVAVDKYQRAKQVEPELGPEADKLIYTYSAYFPATSTIFFYTLKEGDI
                     YRVECWINEDTRIRASKQ"
     gene            complement(9007..10302)
                     /locus_tag="PKM34_00290"
     CDS             complement(9007..10302)
                     /locus_tag="PKM34_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82044.1"
                     /translation="MITQSKGKYLLLGLLLVASFRLSSLYGQISINSPYTRYGLGTLV
                     ENGNDPRITAMGGLYSGFRSPSVINTANPASYTAFDSVSFVFDAGVFGLMSNLQVTNQ
                     KNQGSYVSLSHLLFGFPVTRWWRTSFGVLPFSYVGYDIFNTEQQTGMPDIQYVYRGSG
                     GLNQLYWGNGFKIGKKLSVGFNVKYLFGNITRSRGVTFPDSIEMKNTYIRSSMRPSDF
                     YGEIGFQYREKLSKDLFMVIGGVFGPELEIGSSTSRLVTTYFGDITEAQLYYDTIDAV
                     DNEKGSFTLPMRTGGGITLGKDGKWMAGADFTWQNWEKYRYNGNSDSLVNRWSVGVGG
                     EYIPDSRNIGTYFQRVAYRIGFQYGRTPIYLKGKYIDEFGISFGMGLPIKKSKSTVNL
                     AATIGKRGTIQNGLIQENFIKFTIAVNVFENWFFKSKYY"
     gene            complement(10280..11023)
                     /locus_tag="PKM34_00295"
     CDS             complement(10280..11023)
                     /locus_tag="PKM34_00295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNQ82045.1"
                     /translation="MHRKLIIDIGNTLQKLAVFDGKTMLFKETFDKLKPDSLTGFIAQ
                     HGPFDGIIQSSVIIHTRQLEEILAAAGRFILLTDQTPLPFRNLYRTPKTLGKDRIAAV
                     AGARTLFPGRNCLVIDAGTCITYDLITAEGTYLGGAISPGIRMRFKALHTFTGKLPLI
                     EQEEFDDLIGQTTQESILSGVYNGITGEITHLAGRYHEKSGDLAVVITGGDHHFLHNK
                     LKISIFAAPDLVLLGLNEILDYNDHSIKG"
     gene            11163..11235
                     /locus_tag="PKM34_00300"
     tRNA            11163..11235
                     /locus_tag="PKM34_00300"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11196..11198,aa:Phe,seq:gaa)
     gene            11379..12404
                     /locus_tag="PKM34_00305"
     CDS             11379..12404
                     /locus_tag="PKM34_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82046.1"
                     /translation="MKRISLIILSSLFVLILFQRCAKDDLNGNIASNPKNREIIKEKI
                     NDNRPESEDIAHLYFDFLNRIKSHKDIDKCTIKHDPNFDDSAIDKGMWLIETALNNEY
                     GFKNDSIAYERDLNRFYNIDIIGYDSNSIPIVHGGQLDDVYIEMEQVIINGNQVDTNF
                     WMCTVDLDTIMDNVLTINIQLYRGKIYRLWTVLTPGTEPVPFPNGTSLRSCYWYNGEA
                     PLWYEIESRIRGQHFIVPAPGYIFVYSGHEGASFYTMLEWYPDYRFWGGTSGLEMLNT
                     SICNQYLLSIKEVVDQYNPYPNPDFILGTLIMNFFEYPYGYWHGCDIWHTADFLYFEV
                     VYVGFPN"
     gene            12407..13876
                     /locus_tag="PKM34_00310"
     CDS             12407..13876
                     /locus_tag="PKM34_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82047.1"
                     /translation="MNIYKILGLLVTIILANWPGYGYSQDYSLRISTFEDEFPAGSLS
                     DLNNGIYSIVVRGQFELDPTPADFFHNYRNTIYHINDQGEIIDDIEIDKIDGYDLLLK
                     SIIRTGDSLMVWGNAYRPEDESYSLVILYFNSNMQLLGINIPYTSDSIIEFFCYTAVD
                     QDNLVFSACKGEYGWDQLLVKTSRTGNFIQERLNPGFAGPWPNIGYLPSVDQILCGRY
                     HWLGYIDNTDFTIDSVYYPLSIDLLSYGFFRPYDSSHCIIPSQFYNWDATTDPGYFVI
                     DTIAQATDTALFDLRPDQNEAIDIDYLTIDTMFLGAIENSYMPEPNKGFNPVDRWFAV
                     IKFSKDGEIIWENRFGGEANYILYDITAMQDGSCVMLGTYYDWRNNTMMERDVMIIKS
                     TPDGLYTGLAGHQSPEVQIFPNPANERLFVRISGNSTSSYVIDEITVSDMTGKIWSTS
                     GVNHEKEIMVDVSGYPPGVYYISLFSGNQFLLGKKTVIF"
     gene            complement(13908..15656)
                     /gene="aspS"
                     /locus_tag="PKM34_00315"
     CDS             complement(13908..15656)
                     /gene="aspS"
                     /locus_tag="PKM34_00315"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ82048.1"
                     /translation="MLRSHNCGELRLTDTGKTVTLTGWVQRSRELGGMTFIDLRDRYG
                     LTQLAFNMETNAELCLQARKLGREFVILATGRVIERSSKNLKIPTGEIEIAVEKLEVL
                     NISKVPPFTIEDETDGGEELRMRYRYLDLRRNPLRRNLELRHRMAIETRNYMDSQGFL
                     EIETPYLIKSTPEGARDFVVPSRVHNGKFYALPQSPQTFKQILMVAGYDRYYQIVRCF
                     RDEDLRADRQPEFTQIDCEMAFITQEDILNTFEGLAQHLFRRVKNYETGPFTRMTFAD
                     AMKFYGSDKPDIRFEMKFAELNDLVTGKGFGVFDQAELTVGICAPGCAEYSRKQLDEL
                     TEFVKRPQIGARGLVYIQCKADGTFKSTIDKFYSPEDLKKIAEAFHAGPNDLILILAG
                     DAHRTRKALNELRLEMGNRLGLRDPNVYKPLWVIDFPLLEWDEETKRFYAMHHPFTSP
                     KPEDIPLLETDPGQVRANAYDLVINGVEIGGGSIRIFNKELQKKMFQILGFSDEDAQE
                     QFGFLMNAFEYGAPPHGGIAFGFDRWVAMFSGVDSIREVIAFPKNNAGQDMMIDSPSE
                     ISGDQLKELGITTVGK"
BASE COUNT         4028 a         3514 c         3794 g         4367 t
ORIGIN      
        1 cggtctttcc ggcaggcagg agcgttaacg gatcattcgg atcacgaacc tgtactttca
       61 tccagggtgg cgccctgtat acaccattac cctgcgagta agcctgtgaa agcaattccg
      121 tcataccgta ctcagaatga atacttttcg attgaaaacg ctggcataac aaatgatgaa
      181 gctcttccct gaccatttcc ctgcgcctgc ctttcatccc acccgtttcc ataatgatcg
      241 catgactaag gtcgatagga tgtcgttcgg aaaagtcaag caaagcatac gatgcaccga
      301 acagtattgt ttttattgtc ccttcaacag gctgcaacaa acgggcttcg agagcatcat
      361 gatcgtgcag gaaaaatccg cttccattac gctctgtcat cctgatcagg aaatcgagca
      421 tatacacgag ggaggaaccg gtacgttcga gataccccgg caacaaggcc aggaaacggt
      481 aattggcgac ctctccgtaa aacaacctga acgcttttaa gaagctttcc tcgtaaagcc
      541 ggagatcaca gataaaatgc ctggatggca ccgtaccggt ggtgctgcta ctggtaaaaa
      601 ccttttcggc agatttcgaa tcacctgtta caaccacatg atctttaaaa aaaccgacgg
      661 gcataaaagg aatgtccgtg taatgcgaaa tgacgtccgg atttacgttc aggtaggtta
      721 gaaaacttct gtaaaccggg ttctcttccg cctggtagcg gaaaatttcc aatgccaggc
      781 agttccagtc ctgatcatta cccgggctaa aaatccgtgc tttaatttct tccgaaagca
      841 tcatcaggaa taaaaagttc aggaatgaaa attgcttaat tttgaaaaat caaccgtacg
      901 atgaaatttt ccataaaaat acgttttatt ttggctttag gctttcttac ggccatagta
      961 tcgtgtcagc gaaccgagga atatccggtt gagccgcgca ttacattttt agaattcgaa
     1021 aagattctga atgaaaccga tagtgttttt gacaaagggg tactgaaatt caaatttacc
     1081 gacggtgatg gcgatatcgg cctttcggag ggcgatacct tgccgccgtt caatcccgga
     1141 agcaaatatt attacaattt gctgatcgat tattatgaag tgcgcaatgg agcggaaacc
     1201 tatgtccctc tgacttctta caattcggag accggcctct acgacactgt ttacctgagt
     1261 gccagggtgc cgttgctgac acctgaagga tcgaacaaag ccatttcagg agaaattcat
     1321 gactcactgt tcatttataa ttacaattcc gattttgata ccctttttct gacattcaga
     1381 ttggttgacc gggcgctgaa cgaaagcaac acagaacgga ccccttacat tgtcagatcc
     1441 ttttctccgc aataaccggt catgagaaaa acgctggaaa gcaggaagta cgttttttta
     1501 accctctttg tgattgcagg ggctgtattt accatccggc tattcctgct gcaggtggtg
     1561 caggaaaagt acaaactctc ggccgacaac aatgtcctgc gctatgtgac gcaatatcct
     1621 ccccgagggg tggtttttga ccgtaacggc aagctgatgg tatacaatga agctgcgtat
     1681 gacctgcttg ttatcccaaa gcaggtaaag gctttcgaca cgacggaatt ctgccggctg
     1741 cttgaaatcc ccattgaaga agtaaggctg aggctgaaga aagccaggag ttattcgatg
     1801 gtcaagacga cggtattcct cgaacaatta tcgaaagaag atttcagcgc actggatgag
     1861 gttttgttca aatttccggg ttttttcgta cagctccgta ccgtcaggaa gtatgacact
     1921 cccacagccg cgcatgttct gggatatacc ggtgaagtaa ataacaggga tattgaacgg
     1981 gatccttatt ataaaatggg tgattacatc gggctgaccg gcctggagaa atcctatgaa
     2041 acgatcctgc ggggcaaaaa agggatgaag atcaagctgg tggatgtgca caaccgggag
     2101 atggggagct accaggacgg gaaatacgac agtgcggcgg ttgcaggcaa gaacataacg
     2161 gtttcgatag atgcggaggt ccaggaatat gctgaaaaac tgatgcggtt taaaaggggg
     2221 agtatcgtag ccatagagcc ctctacggga gaaatactgg tgatggtctc cagtccgtcg
     2281 tacgatccga acctcctggt aggaagggga aggtcgaaaa attttgccga gtttaacaag
     2341 gatacgctta aacctctttt taacagggct atccaggcgc aatatcctcc cggatcgacg
     2401 ttcaagactg tcaataccct gatcgggctt caggagggtg tgctgcacga ttacacaagg
     2461 tacggatgca gtggtacatc gaccactcct attccctgca gccacagcca tccttcgccg
     2521 cttgacctgt accatgccat tgagcaatcg tgcaatcctt atttctggca ggttttccgg
     2581 tcaattcttg accagcggaa attccgtacg atgcaggaca gttataatta ctggcgggaa
     2641 gaggtgatga gtttcggtat cgcgaccatt ctggaagggg atgtggttga tcagaaaagc
     2701 gggaacctgc ccaaagacac ttactatgac cggtattacg gcaaaacggg ttggaaaccc
     2761 ctgacgatcc gttcgctggc gatcgggcag ggagaagtgg aacagacgcc gctgcaactg
     2821 gccaacgtgg cggccattat tgccaaccgg ggattttacc atccgccgca tctgctgaaa
     2881 gccgttgagg gtgatacgga accggcgaaa aagttcgaaa agaagatccc tgcccggatc
     2941 gcctcgacct attatgagac gctcatcgag gggatgtcga tggtatataa cggcggcggg
     3001 ggatcggcca ggtattacaa aattgacagc gtggaatgct gcggaaagac aggcacctca
     3061 caaaatccgc acgggaaaaa ccattcggtg tttattgctt tcgctccgcg ggagaatccg
     3121 aagatagcca tagcctgcgt tgtggaaaat tcgggatatg gagcaacctg ggcggcgccg
     3181 attgcttcgc tggtcatgga gaaatacctt aaaaggacaa taaagcagac cgctacggaa
     3241 gaaaggatgt tgaatgctta tttaatagaa ggaagtgacc ccggcgacta aaaacatata
     3301 cggcaagatt gactggacaa ctgtctggct ttacatcatc ctggtgctga tgggatgggt
     3361 cagtgtatat tcatcggtct atgatgaaac tcacagcagc atcctggata tctcgcaacg
     3421 ttacggaaaa cagatgatct ggatcattgc cggcggcgtg atagcgtttg tgatcatgct
     3481 gaccgacgcc aaggtcttca atgcttttgc ttatatcatc tatggtattt ccattctgat
     3541 cctgattgcc gtacttctta tcggcactga gatttcgggt tccaaatcct ggttccagat
     3601 cggcagtgtg gccatacagc ctgccgaatt tgccaaattc gccacctgtt tggccctggc
     3661 cagatacctg agttcctacg ccacggatat gaatgtgctt cagaccaaga ttgtagccac
     3721 tgctttgata ttcacgccgg cgcttctgat atttttacaa aatgataccg gttcggccgt
     3781 tgtgtatatt gcgcttatcc tggtacttta ccgggaaggc ctttcgggat atttcctgat
     3841 ctcgggcgta atcatggtcg tgctttttgt catgaccttg ctgatcaacc agtatattat
     3901 gaccggaatc ttgtcggtgc tggccgttct gctctattat tacaataagc tgctcaggaa
     3961 gcgctggtgg atcattatcg gcatatggct ggcgtcggtt gccatagtat ttgcggtgga
     4021 ttatgtattc gacaacttcc tggaagagca ccagaagacc cgtatcaatg ttttgttggg
     4081 caaggaatct gacccgaaag gcgcgggtta caatgtcaac cagtcgaaaa tagccattgg
     4141 atcgggagga ttgatcggca aggggtttct gcaggggaca caaaccaagt acaattttgt
     4201 gccggagcag agcaccgatt tcattttctg caccgtaggc gaagagtggg gcttcctggg
     4261 aagtacggca gtgataggtt tgttcgttta ccttcttttc aggctggtca ggatggcaga
     4321 gcggcagcgg tcggatttca gcaggatata cggatactgt gtaatatcga tccttttttt
     4381 ccactttgta gtgaatatcg ggatgacgat cgggcttgtg ccggtcatcg ggatccccct
     4441 tccctttttc agttacgggg gatcctccct ctgggcattc accatcctgc tttttatctt
     4501 catcaagctg gatgcgagca ggctggcgtt attctagaaa tacaggagcc tgttgtcttc
     4561 tatctttgta attttctgaa gggcgttgaa cataggatta gctcccatgc gtgagcggaa
     4621 agcggccagc agctgatcgc ggttagcatt gtaatcggcc ggttgcggcg gttcatagaa
     4681 tcgatcgacc accacgacaa acacgccgcc gttaccctgg atgggttcag attgctgtcc
     4741 ggcttccatg ctgaatattt ccccgataac ctggtattcg ctgccaaaac cgggtatatt
     4801 cctggaagag aaggtcagtg tcaggtttgt atccactttg gtattgaatt cccgggcgat
     4861 ctggtaaatg tcggtaccga tgcctttaat acggtcttta agcatagagg ctttcttttc
     4921 attcatcaca aatgatttga tattctcctt catctgttcc agcggcatga tacctttttc
     4981 cctggcggcg gtaacgactg ccaccacgta agcgccaccg atatcgaata ccggtgaaac
     5041 gtcgccgact tcgatcccat caaagaaagc ccagcgtaca atttcgcgcg gataatcgat
     5101 gccggcaata gtattgccca tttcccgcag gtaagtggcc gatcttttgt tcagcccctg
     5161 gctgctgaca gccgcatcga atttagcctg ggtgctgttt tccccggcga aagtgctggc
     5221 ctgtgcgtaa acatcctgga aagttttgct gctgggggca attgttcgtt caataacagc
     5281 taccctgatt ttctgttccg ggtctttctt gccggtaatt ttcaccacat ggtaaccgaa
     5341 agcggtttcc actttaacca catcaccgac tttaccgttg aggaccgcat cattaaaagg
     5401 accgatcatg gcgccatctg agaaccatcc caggtcgccg ttattcgatt tggccgatcc
     5461 gtcgtcggaa aggaggtaag ccagggcctg gagtttggtt ttatcagcgg agacaatcgt
     5521 gaatatactg tcggcggttt tttctgcctg tgttttggta cgggtgacat tttccccggc
     5581 tctcaatgcg ccctggaaag cgatcagaat gtgttcggct ttcatcgaat cggggcgggc
     5641 ctggatatcc atcagccggg ccatatgcca ggcattattc tcctcgtaag gcgggacgaa
     5701 agtgcctgcc ggggcatccc acaaagccac ttcgatgttg agcggaagca atcctttttt
     5761 ataccatgta ctatcgtaac gcttatcaga agttgagttg acaaatgtta caaagtcgct
     5821 cgcattgcgg aactcatcat aaatcctgta agcttcctgg cgcgcatctt cacggtcttt
     5881 tgccgatggt gccacatcaa aaaccaggta atcgatatcc cttgaaggct cctgctcgta
     5941 agattgttta ttcttttcgt aatacgtttc gtaatcttta tcggttagag taacctcatc
     6001 atcattgata acggtataac gggctacaac gtacctcact tctgcgttct ttttcttgct
     6061 ttcaaaatcc agggcagcaa aagcctgagg catgtaatat cctttggtca ccaggctttt
     6121 gtatttttga tcaagccggt cttctttgat atatttttcc aggtcaagcc actgtttacg
     6181 cacctccgga gtcaggttat caaggttctg gagaaagttc tgcacaactg tcgggctgta
     6241 cattcccgtc tgcgggtctt tgaaatattg ctggatcagg ttatgcggac gcggcccctg
     6301 gacgaggtcg taaagctctt cagtagtgac agaaaggccc agctttttat attcattacc
     6361 cataatgatt tcatccaggt actggttcca ggtactctgc cgaatatcaa atgtttctcc
     6421 gggagttaac gattctttat tctgattggc tttttgattt tcaagattta catcgacgcg
     6481 gcggttataa tccgtgaccg tgatctcttc tccggcgatg gtgcctacag ccgggacgcc
     6541 atttcccctg cctcgtttgc ccgagaatag atcgctgacg atgaaagcgg cgatagccac
     6601 gccgacaaga ataacggcga gagccgaatg tttacgaagt gttccgataa tagccataac
     6661 tgcgtattta ttttaaaaag aggtgcaaaa gtataaaaaa aacgaacggg tgcaaagtaa
     6721 ttcatatatt aatgatgcta tccgtcataa tagtaaccgg ttaagcgacg ggtccgggtc
     6781 gtggataagc agaagaacat ccccaaggcg ggttactgcg gttcgtcttt tatttcaagc
     6841 gtaccctgca catgggttat atcaagcaca tcaaatggtt ctttagatgt cagcccggta
     6901 ccggttacca catttccacc tgtcgtgagc ctgacaaact ggttggaata gatcacggct
     6961 ttggcccggt cccagaaaag ctcttcggtg ttaagctttt cctctttctc caggttctcg
     7021 accacgacgt tatgccgggc ttccatcagt ttcttttttt cataggagat tccgtaatca
     7081 gccgtgatct tagtggtcag gttcatggct gtatcgtaga aaaaaacggt gaatccttcg
     7141 gggaacagga gcagttcttc atcgcctttt ttattgacga ggtaagggct gaccagtttg
     7201 atctgcattt gtcccttttc gctgtaaaga atttcaatat cccttgctgt catttcggga
     7261 agagtatcca caaaatccag tgagagaacc gtctgaacat catttttaca ggatgaaaaa
     7321 atcgccggcc ccatcagagc gatgatgccg gcgatgataa tcaacaatat tttattctcc
     7381 ccaagagaag gcctggtttt caaaacgggt gcaattattg ttttgacgcc ctgatccggg
     7441 tatcttcatt gatccagcat tccaccctgt aaatatcacc ttccttcagg gtatagaaaa
     7501 agatagtaga agtggcaggg aaataggccg aataggtgta aatcagttta tcggcttccg
     7561 ggcccagttc cggctcgacc tgtttggccc gctggtactt atcgacagcc acccaatagg
     7621 ccacatggtt ggtcaggtca ttgcctccgc aatccctggc agaggcggca taaaggtcac
     7681 cgatcagcat atagggatcc ccgtcggtcg gatcgagagc agcggccttc aaagcatagg
     7741 ccctggacga aggatagttg ttcagcgcct ggtaagcttt ggcgatatac atgtagcttt
     7801 tctgcactgt agcggtatca ttgagcttct ccgcctgttt aagataatcg atggcctcgg
     7861 catatttccc ttcattcagc agcattttac cgatcatgta agccgattcg ggtgaaggct
     7921 ccaattcata cagcttcact gttgtttcga aatagaaagg atcggtatgg cattttttct
     7981 gttccagcaa attgctgatc ttcttaagca attcgacatc atccggagtt tgattgaatt
     8041 tcttacggta gatggtcacc aggtcggggc aggtggcaaa gggttcgagt ttgaagtcaa
     8101 tactggcttt aatcaatccc caattgccct tatcaggtga aatattttgt tcttgccagc
     8161 ttgttaaaat tccattctca aaatagagat acaaattatc ataaatccac tgttcagttt
     8221 ctccatttga agtaactgat ttgtttacac ttttaggaac acctaaataa actctggtct
     8281 cagaatcatt taaacccact tttaagttct ttgtgatact ttcagatgcc agctttttta
     8341 aattattttc aacgatctgc atagaacttt catatgcatc aaatattaat gatgaatctg
     8401 atcgctcctt agctaggttt attgcagcat ccatataata cggtaaaatt gcacctaacg
     8461 tattgtcccc ttccaattca atagacctat ggagataatt aaaaatattc tctatatctt
     8521 gcggcctata attaaataga tcgacacctt gtcgacctaa aacaaaaccc tcctttccaa
     8581 agtattttat acgttggtca tataacatca tcaatgtgtc aacatatcta ttttttaatt
     8641 tatcaaaata gttaaaagtt tctttattct ctttatattt tagggctgaa tcattggttg
     8701 attcaataag ataacttaga atcttcgcgc catcgatata tgtattctga gttcccctgg
     8761 ggcagttcaa aaagacccat ctccatggcg ggatcaggtc gtggatcgtt tcacttttat
     8821 aatcgctggc tttccactgt ttgaaaaact cccggtacaa tgaaatgttc gtgatgcagg
     8881 tgaggctgtc gctaccgtat ttgccggctc cctggtcttc ctgcccgaaa ctgttcacag
     8941 aagggatacc ggcgaaaatc agtgaaaagg taataacaaa aaataggcgt ttcataatta
     9001 taatgattaa taatatttgc ttttaaagaa ccagttttca aaaacgttga cagcgatcgt
     9061 aaatttaata aaattttcct ggatcaaccc gttctggatg gttcctcttt ttccgatagt
     9121 tgctgcaagg ttcaccgttg atttcgattt ctttatcggt aaacccatcc caaaacttat
     9181 gccaaattcg tcgatgtact ttcccttgag atagattggg gttcttccgt actgaaaacc
     9241 aatgcggtaa gcaactcttt ggaagtaagt tccgatattt ctcgaatccg ggatatattc
     9301 accgcccaca cctacgctcc agcggttgac aagtgaatcg gaattgccgt tatagcggta
     9361 tttctcccag ttttgccatg taaagtcggc gccggccatc cattttccat cctttccgag
     9421 ggtaatgccc cctccggtac gcatggggag cgtgaaactt cctttctcat tatcaacggc
     9481 atcgattgta tcgtaataaa gctgggcttc ggtaatatct ccgaaatagg ttgtcacaag
     9541 ccttgaggtc gaagatccga tctcaagttc gggaccgaac acgccgccga tgaccataaa
     9601 cagatccttt gaaagttttt ccctgtactg gaaaccgatc tctccataaa aatcgctcgg
     9661 gcgcatagag ctgcggatgt aggtattttt catctcgatg gaatcgggga aagtgacgcc
     9721 ccggctgcgg gtaatgttcc cgaacagata ttttacatta aatccaaccg aaagtttttt
     9781 accaatctta aacccattac cccagtaaag ctggttcaat ccgcccgaac cgcggtaaac
     9841 atactggata tcgggcatgc cggtttgctg ttccgtattg aaaatatcgt atcccacata
     9901 actgaaaggg agcacgccga agctggtgcg ccaccagcgt gtcaccggga aaccaaagag
     9961 aagatgactg agggaaacat agctgccctg gtttttctgg ttggtgacct gcaggttcga
    10021 catcaaaccg aacaccccgg cgtcgaacac aaagctgacc gaatcgaaag cggtatagga
    10081 agcgggattg gccgtattga tcacactggg cgagcggaaa ccggaataga gtccgcccat
    10141 ggcggtaata cgcggatcat tgccgttttc taccagtgta cccaatccgt agcgggtata
    10201 cggcgagttg atactgatct gaccgtataa ggaggacaac cggaaggagg caaccagtaa
    10261 aagccctaat aacaagtatt taccctttga ttgagtgatc attatagtcc aaaatttcgt
    10321 tcaatcccag cagcactaaa tcgggggctg caaagatgct aatttttagt ttattatgca
    10381 agaaatgatg atcaccgccg gtaataacca cggccaggtc accggatttt tcatggtagc
    10441 ggccggcaag atgagtgatt tcgcccgtga tgccgttata aaccccggaa aggatagatt
    10501 cctgtgttgt ttgtccgata aggtcatcaa attcttcctg ttcaattaac ggcaatttac
    10561 cggtgaaagt atgcaacgcc ttaaaccgca tccggattcc gggcgatatt gctccgccca
    10621 ggtaagttcc ttcggcggtg atcaggtcgt aggtgatgca ggtgccggcg tcgatcacca
    10681 ggcaattccg gccgggaaag agtgtacggg ccccggcgac ggcagcgata cggtcttttc
    10741 cgagtgtttt cggtgtccgg taaaggtttc ggaacggcag cggcgtttga tcggtcagga
    10801 ggataaacct gccggcggcg gccaggatct cttccaattg acgtgtatgg ataatgaccg
    10861 acgactggat gatgccatca aaagggccgt gttgtgcaat aaaaccggta agggaatcgg
    10921 gtttcagctt gtcgaaggtc tctttgaaca gcatggtctt cccatcaaaa acagcgagtt
    10981 tctgcaaggt attcccgatg tcgataatga gttttctgtg caaggtttat agtaaagtgt
    11041 gcaaagatag catttttcag tcggcagtcg gcagtcggca gtcagcagtc ggcagtcgac
    11101 tttttttgcc cgtgttatat tatttaccaa aaaatgtata tctttgcgct cccaattttg
    11161 ctggtaccat agctcagttg gtagagcaac ggactgaaaa tccgtgtgtc gctggttcga
    11221 ctcccgctgg taccaccggt agcctgattt tccagaaagg aagatcaggt tttttatatt
    11281 tattccggca agattcttct tgacttttga tattgaattg attatatttg ctctctttgc
    11341 taaaagtcat tccgtattaa tcaattaatt caaaaatcat gaaaaggatt agtttaatta
    11401 ttctatcaag tctttttgtt ttgatattgt ttcaaaggtg cgcaaaagat gaccttaatg
    11461 gaaatattgc gtcaaatcca aagaatcggg aaatcattaa agaaaaaatt aatgataata
    11521 ggccagaatc cgaagacatt gcccatttat atttcgattt tctaaatcga attaaaagcc
    11581 ataaagatat agataagtgt accattaaac atgatccaaa tttcgatgat tcggctattg
    11641 ataaaggtat gtggttgatt gaaacggcat taaataacga atacggattt aaaaatgata
    11701 gcattgcata tgagcgagat ctgaatcgat tttacaacat tgatattata ggatatgatt
    11761 caaacagtat tccaatagta catggagggc aacttgatga tgtctatatt gagatggaac
    11821 aagttataat taatggaaac caggtggata cgaatttctg gatgtgtaca gttgatctcg
    11881 atacaattat ggataatgta ttaacaatta acattcaact ttaccgtgga aaaatatatc
    11941 gattgtggac agttttgact cccggaacag aacctgttcc ctttcctaat ggaacgagtc
    12001 tacgctcttg ctattggtat aatggtgagg ctccattgtg gtatgaaata gaatcaagaa
    12061 tcagggggca gcattttatt gtgcctgcac caggatatat attcgtttat tctggccatg
    12121 aaggtgccag tttttatact atgttagagt ggtatcctga ttatcggttc tggggtggca
    12181 catcaggatt ggaaatgctt aacacatcga tatgtaatca atatcttctt tccataaaag
    12241 aagttgtgga tcaatataat ccatatccaa atcctgattt tattcttggt accttaataa
    12301 tgaatttttt tgaatatcct tatggttatt ggcatggttg tgacatttgg catactgcgg
    12361 attttttata tttcgaagtt gtttatgtag gatttccaaa ttaaacatga atatatataa
    12421 aatattagga cttctggtga ccattatttt ggcaaattgg ccaggttacg gttattctca
    12481 ggattactca ttacgcatca gcactttcga agatgaattt cctgccggga gtctttctga
    12541 tttaaataat gggatttatt caattgtcgt acgtggtcaa tttgaattgg atcccacacc
    12601 tgctgatttt tttcataatt accgcaatac catataccat atcaatgacc agggagaaat
    12661 aatagatgat attgagattg ataagattga cgggtatgac ctgcttctta aaagtataat
    12721 cagaaccggt gatagcctga tggtttgggg gaatgcctat cggccggaag atgaatcata
    12781 cagcctggta atactatatt ttaacagtaa tatgcagctt cttgggatca atattccata
    12841 cacatccgac agtataattg aattcttttg ctatacggct gtcgatcagg ataacctggt
    12901 cttttccgct tgtaaaggtg aatatggctg ggaccagtta ctggtcaaga ccagccgaac
    12961 cgggaatttc atccaggaga ggttgaatcc tggttttgca ggtccatggc caaatatcgg
    13021 ttaccttcct tctgtcgacc agattttatg cggcaggtac cattggttag gttacatcga
    13081 taatacggat tttactattg attctgtcta ttacccgctt tcgatcgatc ttttgagtta
    13141 tggttttttc cggccttacg atagttccca ttgcattata ccgtcacagt tttataattg
    13201 ggatgcaacg accgatccgg gttattttgt aatagataca attgctcagg caaccgatac
    13261 tgcattattc gatctccgtc ccgatcagaa tgaggccatc gatatcgatt acctgaccat
    13321 cgatacgatg tttctgggag ccattgaaaa ttcgtatatg ccggagccca ataagggatt
    13381 caatccggtc gaccgatggt ttgccgtgat taaattcagc aaggacggcg aaattatctg
    13441 ggaaaaccgt ttcggaggag aagccaacta tatcctttac gacattaccg caatgcaaga
    13501 cggcagttgt gttatgctcg gaacatacta cgactggcgc aataatacga tgatggaaag
    13561 agatgtaatg atcataaaat ctactcccga cggcctttat acgggacttg caggccatca
    13621 aagcccggag gttcagattt tcccaaatcc ggccaatgaa agattattcg tcaggattag
    13681 cggaaattca acttcatctt atgtcattga tgaaattacg gtatcggata tgaccggaaa
    13741 aatatggtcg acttccggtg ttaaccatga aaaagaaatc atggtggatg tttccggtta
    13801 tcccccggga gtttactata tcagcttatt ctccggaaat caattcttgt tgggcaagaa
    13861 aacagtgatc ttctgaattt ctcttactta ggttcaaatt ttgaagttta tttgccaacc
    13921 gtggtaatcc ccagctcttt cagttgatcg ccggagattt ccgaggggga gtcgatcatc
    13981 atgtcctggc cggcgttgtt tttcgggaag gcgatgacct cgcggatcga gtcgacgccg
    14041 ctgaacatgg ctacccagcg gtcgaatccg aaagcgatgc cgccatgggg cggggcgcca
    14101 tattcgaaag cgttcatcag gaatccgaac tgctcctggg cgtcttcatc gctaaagccg
    14161 aggatctgga acatcttctt ctgcaattcc ttgttaaaga tacggatcga acctcctccg
    14221 atctccacac cgttgatcac caggtcgtag gcattggcgc ggacctggcc cggatcggtt
    14281 tccagcagag gaatatcctc cggttttggc gaggtgaacg ggtggtgcat ggcatagaaa
    14341 cgcttcgttt cctcgtccca ttcaagcagt ggaaagtcga tgacccataa gggtttgtag
    14401 acattggggt cgcgcagtcc cagccggttg cccatttcaa gcctcagttc attcagggcc
    14461 ttacgcgttc tatgcgcatc gccggcgagg atcaggatca ggtcgttggg cccggcatgg
    14521 aaggcttccg cgatcttttt caggtcttcc ggcgaataga atttatcgat ggtcgatttg
    14581 aaggtgccgt cggctttgca ttggatatag accagtccac gggcgccgat ctgcgggcgt
    14641 ttgacgaatt cggtcagctc gtcgagctgc ttgcgggagt attcggcaca ccccggggca
    14701 caaatgccga cggtcagctc tgcctggtca aaaacgccga aaccttttcc tgtcaccagg
    14761 tcgtttaatt cggcgaattt catctcaaag cggatatcag gtttatcgga accgtagaat
    14821 ttcatggcat cggcaaaggt catgcgggta aacgggccgg tctcgtagtt tttcactctc
    14881 ctgaaaagat gttgtgccag gccttcgaag gtattcagga tatcttcctg ggtgatgaag
    14941 gccatttcgc agtcgatctg ggtgaattcg ggctggcggt cggcgcggag gtcctcatcg
    15001 cggaaacaac gcacgatctg gtagtagcgg tcgtatcccg ccaccatcag gatctgtttg
    15061 aaagtctggg gcgactgagg gagggcatag aacttgccgt tatgcacccg ggagggaacg
    15121 acgaaatcgc gtgctccttc gggagtggat ttgatcaggt agggtgtctc gatttcgagg
    15181 aatccctgcg agtccatgta gttacgggtt tcgatggcca tccggtggcg cagctcgagg
    15241 ttccggcgca ggggatttcg gcgcaggtcg aggtagcggt agcgcatgcg gagctcttcg
    15301 ccgccgtcgg tctcgtcttc aatggtgaag ggaggaactt tcgatatgtt gagcacttcg
    15361 agcttttcga cggcaatttc gatctcaccc gtaggaatct taagattctt gctgctgcgt
    15421 tcgatgactc ttccagtggc aaggatcacg aattcacggc ccagcttcct ggcctggagg
    15481 cacagttcgg cattggtttc catgttgaag gccagttgcg taaggccgta gcggtcgcgc
    15541 aggtcgataa aggtcattcc gccgagctcg cgtgagcgtt gcacccagcc ggtgagggtg
    15601 actgttttac cggtatctgt caggcgaagt tcgccgcagt tgtgggatcg aagcatagtc
    15661 gttggattat aatttgggtg cgaagatagg aaaaagttgg cag
//
LOCUS       DAOQXS010000005        15089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4411_length_15089_cov_4.388453, whole genome shotgun sequence.
ACCESSION   DAOQXS010000005 DAOQXS010000000
VERSION     DAOQXS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4411_length_15089_cov_4.388453"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKM34_00320"
     CDS             <1..471
                     /locus_tag="PKM34_00320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNQ82049.1"
                     /translation="NVRFLRHPHYKRTRYNGFSRRTTEKSEKMFEYNFSVRGYELDSY
                     GHVNHAVYLNYLEQARWELFRNLGLLGCFHENDLLLVVIDVHMRYSREISLFDEVIVR
                     TGITSEPPYLVFHHKMYLKGSNLKICSATIKTLFTDKGKLVRDIPKSITDKLIT"
     gene            471..1208
                     /locus_tag="PKM34_00325"
     CDS             471..1208
                     /locus_tag="PKM34_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNQ82050.1"
                     /translation="MEVSWEKIWERSGSIGILELQGPKGNQLEEPAFLPLPLLEELFG
                     EPGLKGVIIRGSGRHFSAGANLEHLKKLALDEVLLAEKMTAGKETIRYIESSDLPVVA
                     EISGICFGGGLEIALACHFRICSENALFAFPEINHGLMPGLGGTVMLSRLIGQAKAAG
                     MILSGDTVDAEKAMALGLADYVLPAAGLHAFTLDFLQKLTDDRDPGVIRSIMRSIHNA
                     REMDTESALAEETRLFCALAVNNFHSG"
     gene            1208..1585
                     /locus_tag="PKM34_00330"
     CDS             1208..1585
                     /locus_tag="PKM34_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82051.1"
                     /translation="METITEKNIFTDIISREDFILLFSSSEDKENWFTEKELETIGRK
                     GLSGSLAARYLIRKRIAYQVGINIAVTEIEILNDPSGKPEIRFSERWHTALSKAGFQS
                     VVCSLSHSRNFITSLTIFLSNGA"
     gene            1575..2210
                     /locus_tag="PKM34_00335"
     CDS             1575..2210
                     /locus_tag="PKM34_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNQ82052.1"
                     /translation="MELKTIRWITEDGIGQLALDQPPSNTMDRRFFDELSLLTRMVIP
                     GSGIRALVVYGTGRHFSSGADPADLCRRVRETLPSNYPQEIPAFLSMARDCFQYIEKL
                     PVPTFAAIRGVCLGSALELALACRFRICAEGAVFGFPESSFGLMPGCGGTVMLTKAAG
                     SQKAIELLLGGRNFSAGEALEMGLVHRIVSRKTVVEETLKIAKGMISTLSL"
     gene            2207..3325
                     /locus_tag="PKM34_00340"
     CDS             2207..3325
                     /locus_tag="PKM34_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HNQ82053.1"
                     /translation="MSHILPETIRVAGTGSYLPGRPLSIDEAEQVLGELHEAPEKIRA
                     WMKRLKMMMKELLEIERYYYAIDPVTRQFTDDNITMSVKAAQRALEMAGKTPGDVEFI
                     VYGSAHMDQMPTPSVRIQEALGISRCAETSVHANCTSAYKALLIAHELIRSGRYRNTL
                     VISSGISSSELRAEYYNQAVVKKEELFLRYYLGDGAGAVFLEAGNGEEGLYLESTYME
                     SVGGDKPSAMNNRRPAYWMNPKEEYEKGYHHLAQMFRDELARNFHESGGSVFLHGLER
                     YLGKYPADLSKLKYFQVNFPSRHISELIMEECETLGIAKEKLYSKMSGLGYPGPPMAF
                     ICLDNILRNEKLNPGDLVLSFVTEVSKFMQAGYLMEYR"
     gene            3340..4392
                     /locus_tag="PKM34_00345"
     CDS             3340..4392
                     /locus_tag="PKM34_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ82054.1"
                     /translation="MKKIKHIGIVGEGKMGSSIFLFLLGYDFRLTWLCSGSMGKEKAA
                     KQAAKKINILLKSGILTDAAFRKKNEETKITETPGDLADCDLVIEAISEDLETKKEFF
                     RNLDLMVGRKCIFTTNSSSINPSMLIPSESRQERFAGMHFFFPVNLKNTVELIQGPDT
                     SEETVGILKDFLRLIHKNIFMQDEKHAFILNRLLLDFQAEAFNICKDGDISYRQMDDL
                     VKERFFPAGVFEFFDHVGIDVMLASIRNYIKNEPDKSLYLPIVARMKELIKNNELGLK
                     TRKGFYDYELQGRPCDDDEMQNILPAGRLGEIEKRLRQRLEGAYRSVLATGCVSAETF
                     DEAVKDYLGTDQCLPW"
     gene            4461..5309
                     /locus_tag="PKM34_00350"
     CDS             4461..5309
                     /locus_tag="PKM34_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ82055.1"
                     /translation="MNKKVRIARLSIASNTLLIIMKFIVGMISGSVSILSEAIHSLMD
                     LVAAVIAYFSVRVSDNPPDKRHPYGHGKFENLSGVLEALLILVAAAWIVYEATRKMTG
                     KEPVDSIGLGSLVMFISAGVNIFVSRKLYKVAKETDSVALEADALHLKTDVYTSLGVG
                     IGLLLIWITKIHIIDPIIAILVAVIILKEAYELLARAFSPLLDTSIKLADLKKLEDSL
                     VKMGVKYHDLKTRKAGSYIFVEFHLEMPPDKKLEEVHAYCDRIEEELKKQISNLRITI
                     HAEPEE"
     gene            complement(5354..6046)
                     /locus_tag="PKM34_00355"
     CDS             complement(5354..6046)
                     /locus_tag="PKM34_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ82056.1"
                     /translation="MVTRSRILYVEDDETLGFITSENLERKGYEVILCKDGESAYRLF
                     KTESFDLCLFDVMLPKLDGFTLARLVRKENEEIPIIFISAKTLTEDKIEGLLLGADDY
                     LVKPFNIEELTLKIEVFLRRSRVNKAESPESEIRIGSCTLDMGLLRLECGGIVKKLTF
                     REAELISYFSRNRNKLLSREQILEAVWGGNDYFSGRSLDVFISRLRKYFQDDKLVSIE
                     NRHGLGYVFEVK"
     gene            complement(6048..7316)
                     /locus_tag="PKM34_00360"
     CDS             complement(6048..7316)
                     /locus_tag="PKM34_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ82057.1"
                     /translation="MTNRSVQWVIGLGILAILAILAGQAYFFYQAFNLRERQTVQSFR
                     ISLQSVAESISQFNKSTLPDNIIHQYSPDYFIVDINNRIDPWVLEHYLRTELMRRHLD
                     VDFEYAIYDCDDDRMVYGNYVSLDQTEEVKNKLEYWPKYEEGLYYFGVHFPGMRNYLL
                     GEMGLWYFFTGILVMVILFFGYSMMIILRQKKLSEIQRDFINNMSHEFRTPLTSIGLA
                     SDVVREEGPGGDTARFDKYASILRDQIILLQKKVDKILVHAETENRFFRLNKQKFDPQ
                     DVVTEVLEELRPSAEHAGGTLRFLPAAQSSFILADRYHFSGILINLIDNSLKYTSKPP
                     DVCITLSANPKRVFIKLEDKGTGIDSKYLRKVFMPFFRVPSGNIHNVKGTGLGLSYVK
                     QVCDLHGWKVKIESAPGQGTTVTISIPKTE"
     gene            7470..7877
                     /locus_tag="PKM34_00365"
     CDS             7470..7877
                     /locus_tag="PKM34_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ82058.1"
                     /translation="MKKIAALFVLVLVGVQIISAQTQAVQQQPAKTYSWNETTHDFGK
                     IVVNDPATVTFTVKNNGTAPLLMTAAKSSCGCTVAEYTKEPIKPGETGIVKATYNAAR
                     AGSFTKTVTVTFEGISSGELLTIKGEVIAKPEN"
     gene            complement(7843..8634)
                     /locus_tag="PKM34_00370"
     CDS             complement(7843..8634)
                     /locus_tag="PKM34_00370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNQ82059.1"
                     /translation="MNFLYIISAAFVLVVLVLLVRYAWGIFFDSNYRPVEWEYAVING
                     RVSKELEKLERNYPDKVRFFNWWFQVERLKRDGIPGDFAELGVYKGESARIIHMMDPS
                     RTFHLYDTFEGFKEEDLENETGEAATYTTRNFADTSLEAVKRYLGEEGKFVYHAGRFD
                     FSAPTPSLPPAGGEGEREGAYALVNLDADLYNPTKAGLEYFYPRLSPGGVIFIHDYTH
                     KWPGIKRATDEFISKIPETLVHVPDMDGTVMIVKISSPALQLPRL"
     gene            8771..10060
                     /locus_tag="PKM34_00375"
     CDS             8771..10060
                     /locus_tag="PKM34_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82060.1"
                     /translation="MSSKNVIIKISQKKIWFNESVSIAVECSNLPVDDIKFSRVNEIY
                     WLTEQVSFEKDTGLLEVNIQDYFLRDFSSFTAQNPKSLVKSIRFRNLSDTDSLKTALR
                     YYSSGMNHLDQAMEEAFRELSDKPEPIPEKVWKKNIMFRSYQEKEKLEVTFDAWFKDA
                     EFKLGYVAFDRNIPELKQMLRFKVDNDFLLPEFEFIKSYFVKALGTRKFDVKATIFLE
                     HGKPVETSAASVLIKKIDENLIDSIKNIRTAALFKPPFKVNVDKSLFSADEVFGEFGP
                     GEAGNVFSQTEEEMLGQILEKYKVRNRKQLEFLAGVKQAAGSRLKFTLNPYFGFLFTV
                     EGEKMNHFIWELLNSHATYIWSLDKGDGAMARQYRRVEDAINQVRNSGRDNYKQAWRA
                     SHIDHDLVFHVIHHDDAGSAMIDGFVKWRHRLGEVMI"
     gene            complement(10143..10898)
                     /locus_tag="PKM34_00380"
     CDS             complement(10143..10898)
                     /locus_tag="PKM34_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ82061.1"
                     /translation="MQEKIKVIIVDDEDLARELVKKYLAGLADAELLCECSNGFEALK
                     AIQELRPDLVFLDIQMPKIDGFELLEVLDEKPAIIFTTAFDQYAIRAFELNAVDYLLK
                     PFSRERFVQAYEKAKLRLGTTPQGMASQGTPSLLKHLDDTRQTLDRVITRLGSKITVI
                     PVDKIRYIESADDYVMIHSELGDHLKEKTMKYFEEHLPKEQYIRIHRKYIVNINEIRS
                     LELYTKDSYVALLKTGEKLNVSSEGYRRLRERF"
     gene            complement(10882..11937)
                     /locus_tag="PKM34_00385"
     CDS             complement(10882..11937)
                     /locus_tag="PKM34_00385"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ82062.1"
                     /translation="MARGIKNHLEIVYLIMWVTLGVIHFSLLYFGYGLRSVHAFADSL
                     VFNGLFTVIGSGLWFMVRYSNLQTKTKAELALYHITGAAVAIFVWMMAGHLILNTLFP
                     EDQAYQSFLKNTVFLRILAGLMFYALLVSIFYLLINFRELKEKSQREARLTGLLKEAE
                     LNLLRSQIRPHFLFNSLNSISALTMTDPGKAQEMVIKLSEFMRYSLNFPDNMTGTLER
                     ELYNAELYLDIEKVRFGSRLAFEKEIQPEAMSWIVPVMILQPLVENAVKYGVYESEET
                     TVIRLGARILESELEIRIGNTYGFGTMAKKGTGTGLRNIRGRLLNLYGTGSLMKINQT
                     ENYFEVTIRIPEYARKD"
     gene            complement(11957..12709)
                     /locus_tag="PKM34_00390"
     CDS             complement(11957..12709)
                     /locus_tag="PKM34_00390"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNQ82063.1"
                     /translation="MENNTQPAVRHGLKGKSLVFGVLVILLGIALFFRNLEPFEGARI
                     WDVIFSWQMLLIAIGLVNIVNDSSRRVGWILIAIGGFFMIPVIFDIENYRSTFWPALL
                     IIIGLILIFGSSRLCKRSYSHNKTWTDSSGFSSGTITDDFVEEVAVFGGRERFINSQS
                     LKGAKVVSVFGGSTLDMTKVELAPGNVEIEIVAIFGGSTLVVPNDWNIKLEVFNIFGG
                     YSDKRIRGQVDFNKTVIVKGVAIFGGGEIKSY"
     gene            12900..14294
                     /gene="xseA"
                     /locus_tag="PKM34_00395"
     CDS             12900..14294
                     /gene="xseA"
                     /locus_tag="PKM34_00395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNQ82064.1"
                     /translation="MTGNHHNGAEIMTLSQLAAGITVVLEENFRSPLWIKAEIAKLNY
                     YPASGHCYPSLVEKEKGSVKAELRGTIWANDYVRINSNFLKVIREPLKEGIQILFRAY
                     VRFHAVYGLSLQIIDIDSSYTLGEMAREKMETLARLKGEGLFDRNRQLELPALPKRIA
                     VISVKSSKGFSDFINIIENNPWKYGISCVLFPALLQGDKAVESIRSQMELIGRYAHLF
                     DAVAIIRGGGGEVGLNCYDNYMLARDVALSQLPVLTGIGHSTNETVVEMIAGYNKITP
                     TDVAYFIIQQFHNFSGRLNELHENLATIANDILEEEKKRWEVFCLELATQTENLVRNE
                     KRAILELSGSVIDGTGILITGQKHLLNLAGLKLSYKPAGLLRESRTGLENHWERFFLH
                     VKQLLKQKNSEIENLNHKINLLNPVHVLKRGYSITYLNGKALIDCSEAKAGDNLTTRL
                     YKGEISSEVKSLKD"
     gene            14299..14499
                     /gene="xseB"
                     /locus_tag="PKM34_00400"
     CDS             14299..14499
                     /gene="xseB"
                     /locus_tag="PKM34_00400"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNQ82065.1"
                     /translation="MEKQITYTEAMKELELIVGEIEEGEITIDSLSEKVKRASELIRI
                     CKEKLTATEEDVSKILEGLKET"
     gene            complement(14631..15002)
                     /locus_tag="PKM34_00405"
     CDS             complement(14631..15002)
                     /locus_tag="PKM34_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNQ82066.1"
                     /translation="MEFDDLSRKIIGCALQVHKILGPGMLESAYEECLAYELTSTGLM
                     IHRQKPIPIIYKDIKLDCGYRLDLLVEQKIIIELKSVELLAPVHEAQILTYMRFAEIR
                     LGLLINFNVTILKSGIKRYVL"
BASE COUNT         3987 a         3621 c         3699 g         3782 t
ORIGIN      
        1 aatgtcagat ttttgagaca ccctcactac aaacgtacac gatacaacgg cttcagtcgt
       61 cggactaccg aaaaaagtga aaagatgttc gaatataatt tcagcgtaag aggttatgag
      121 cttgattcat acggtcatgt gaaccatgcc gtttacctga attatcttga gcaggcgcga
      181 tgggagctat tccggaacct cggcctcctc ggttgttttc atgaaaacga cctgttgctt
      241 gtggtgatcg atgtgcatat gaggtacagc cgggaaattt cacttttcga cgaagtgatc
      301 gtcaggaccg ggattacaag tgaacctcct tatcttgttt ttcatcataa gatgtattta
      361 aaaggatcga acctgaagat ctgctctgca accattaaga cactttttac cgacaagggg
      421 aaactggtaa gggatatccc gaaaagcatt accgataaac tgatcacctg atggaagttt
      481 catgggaaaa gatatgggaa agatcggggt cgatcgggat cctggagttg caggggccca
      541 aaggaaatca gctggaagaa ccggcatttt tgccgttgcc attgctggaa gagctattcg
      601 gcgaacccgg tttgaaaggg gtgatcatcc ggggaagcgg gcgtcatttt tcggcaggcg
      661 ccaacctgga acacttaaag aaactggctc tggacgaagt ccttctggcg gagaagatga
      721 ccgccggcaa ggagaccatc cggtatatcg aatcatcgga tttacctgtt gttgcggaga
      781 tcagcgggat ttgcttcggt ggcggactgg agatcgcact ggcctgccat ttcaggattt
      841 gctcggagaa cgcccttttt gcattccccg agatcaacca cggcctgatg ccgggtctgg
      901 gcggaacggt catgctttcc cggctgatcg gccaggctaa ggcggccgga atgatccttt
      961 cgggtgacac tgtcgatgct gagaaagcga tggcgctggg ccttgccgat tatgttttgc
     1021 ctgctgccgg tcttcatgcc ttcacccttg attttcttca aaaattaaca gatgaccgtg
     1081 atcccggagt gatccgttcc atcatgagat ccattcacaa tgcccgggaa atggatacgg
     1141 aaagcgccct ggcggaggag acccggcttt tctgcgctct ggcggtcaat aattttcatt
     1201 caggctgatg gagactatca cggaaaaaaa cattttcacc gatatcatat ccagggaaga
     1261 ttttatcctt ctgttttcat cttcggaaga taaggaaaac tggtttacgg aaaaggaatt
     1321 ggaaaccatt ggcaggaaag ggttatccgg tagcctggcc gcccgttacc tcatcaggaa
     1381 aaggattgct taccaggtcg gaataaatat cgcggtgacg gaaatagaga ttctgaacga
     1441 tccttctggc aaaccggaga tccggttcag tgagcggtgg catacggccc tcagcaaggc
     1501 cggtttccaa tctgttgtct gctctctttc gcattcccgg aattttatca ccagcctgac
     1561 tatttttctc agcaatggag cttaagacca tccgttggat aacagaagat ggcatcggcc
     1621 aactggcgct tgaccagccg ccgtcgaaca ccatggaccg ccggtttttc gacgagttga
     1681 gcctgctgac ccggatggtc attcccggtt cagggatcag ggccctggtg gtttatggaa
     1741 cgggccgaca tttttcctcg ggcgctgatc ctgccgacct gtgccggcgg gtaagggaaa
     1801 cccttccgtc taactacccg caggagatcc cggcttttct ctccatggca cgtgattgtt
     1861 ttcaatacat agaaaaactt ccggtaccga cattcgctgc gatccggggc gtctgtctcg
     1921 gatcggcgct ggagctggcc ctggcctgcc ggttcaggat ttgtgcagag ggtgcggttt
     1981 tcgggttccc ggaaagctcc ttcggtttga tgcctggttg cggcggaacg gtcatgctga
     2041 ccaaagcagc gggcagtcaa aaagccatcg aactgctcct gggcggacgg aatttctccg
     2101 ccggcgaggc cctggagatg ggcctggtcc accggatcgt ttcgagaaaa accgtcgtgg
     2161 aagagacatt aaaaatagca aagggaatga tttcaacact atcattatga gtcacatcct
     2221 accggaaacc attcgtgtcg ccggcaccgg cagctacctg cccggcaggc ccctgtcgat
     2281 cgatgaggcc gaacaggtcc tcggcgaatt gcatgaagca cccgaaaaga tcagggcctg
     2341 gatgaagcgc ctgaagatga tgatgaaaga attgctggag atcgaacggt actactatgc
     2401 catcgatccg gtaacgcggc aattcaccga tgacaacatc accatgagtg tgaaagcggc
     2461 gcaacgtgca ctggaaatgg cgggcaaaac tcccggtgac gtggaattta tcgtttacgg
     2521 cagcgcccat atggaccaga tgccgacgcc atcggtcagg atccaggaag cgcttggcat
     2581 aagccggtgt gctgagacgt cggtccatgc caactgcacc tccgcttaca aagcgctgct
     2641 catcgcccac gaactcatcc ggagcgggag gtaccggaac acgctggtca tctcttcggg
     2701 tatttcgtcg tcggagctca gggccgaata ttacaaccag gcggtggtga aaaaggaaga
     2761 gctcttttta aggtactatt tgggcgacgg agcgggggct gtgtttcttg aggcaggaaa
     2821 cggagaagaa gggctttacc tggagagcac ctatatggaa tcggtcggtg gcgacaagcc
     2881 cagcgccatg aacaaccgcc ggcccgccta ctggatgaac ccgaaggagg agtacgagaa
     2941 aggttaccat cacctcgcgc agatgtttcg cgacgagctg gcccgcaact ttcatgaatc
     3001 cgggggaagt gttttccttc acgggctgga gcggtacctc ggaaaatacc cggcggatct
     3061 ttcaaaactg aaatatttcc aggtcaactt cccttcccgt cacatctccg aactgatcat
     3121 ggaggaatgt gagacgctcg gcatcgctaa agaaaaactg tattcgaaaa tgtcaggcct
     3181 gggttatccc ggaccgccga tggcgtttat ctgcctcgac aatatcctca ggaacgaaaa
     3241 actcaacccg ggcgacctgg tcctgagttt tgtcaccgaa gtgtctaaat tcatgcaggc
     3301 gggttatctg atggaatacc gttaaatttg actttttaat tgaaaaaaat aaaacatatt
     3361 ggcattgttg gagaaggaaa gatggggtca tccatattcc tgtttctcct ggggtatgat
     3421 ttccgtctta cctggctttg cagcggcagt atgggcaaag agaaggcagc gaagcaggcg
     3481 gcgaaaaaga tcaatatctt actgaaaagc gggatcctga ccgatgcggc tttccggaaa
     3541 aaaaacgaag agacgaaaat aacagaaaca cctggcgacc tggccgattg cgacctggtg
     3601 attgaagcca tttccgaaga tctggaaacg aagaaggaat ttttccgcaa ccttgacctc
     3661 atggtgggtc ggaaatgcat ttttacaacg aattcatcct ccataaaccc atccatgtta
     3721 atcccttccg aatccaggca ggagcggttt gcaggtatgc atttcttctt tccggtaaat
     3781 ttgaagaaca cggtcgagtt gattcagggt cctgatactt cggaagaaac cgttggcata
     3841 ctgaaggatt ttctaagact tattcataaa aatattttca tgcaggatga aaaacatgcg
     3901 tttatcctga accgtcttct ccttgatttc caggccgaag catttaatat ttgtaaagac
     3961 ggcgacattt cgtaccgcca gatggatgat ctcgtgaagg agcgattttt cccggccggg
     4021 gtctttgaat ttttcgacca tgtgggcatc gatgtcatgc tggcctccat ccggaattac
     4081 attaaaaatg aaccggataa aagtctttac ctcccgatag tggcccggat gaaagagctg
     4141 atcaaaaata atgaacttgg attaaaaacc cggaagggat tctatgacta cgaacttcag
     4201 ggccggcctt gcgatgatga tgagatgcaa aatattttac ctgctggaag gttaggtgag
     4261 attgaaaaac gactcaggca aaggctggag ggggcttaca gaagtgtgct tgctacgggt
     4321 tgcgtgtcag cagaaacatt tgatgaggct gtaaaagatt atctgggtac agatcagtgt
     4381 cttccctggt aaaaattggt aaaaacacac tctcgccttg ccttttatta atagtacatt
     4441 tgacttaaaa tttattctta atgaataaga aagtcagaat agcccgtctt tccatcgctt
     4501 ccaatacttt attgattatc atgaagttta ttgtaggtat gatcagcggt tctgtcagta
     4561 ttttgtcgga agccatacat tctctgatgg acctggttgc agcagttatt gcctatttct
     4621 cggttcgagt atccgacaat cctcccgata aacggcaccc ctatggccat ggaaaattcg
     4681 aaaacctttc cggcgttctt gaagcattgc tgattcttgt cgccgccgcc tggatcgttt
     4741 atgaagcaac ccgtaaaatg accggcaagg aacctgtgga ttcgatcggg cttggatcat
     4801 tggttatgtt catctcggcc ggggtaaata tttttgtgtc aaggaagctg tataaagtag
     4861 ccaaagagac cgattctgtg gcccttgaag cagacgccct gcacctcaag accgatgttt
     4921 acacttccct tggagtcgga atcggattgc tgcttatatg gatcaccaag atccatatca
     4981 tcgaccccat catagccatc ctggtggctg ttattattct gaaagaagcc tatgaattat
     5041 tagccagggc cttttcccca ttgcttgata cttcgattaa actggctgat cttaaaaaac
     5101 tggaagattc gctggtaaaa atgggtgtaa aatatcacga tctgaaaacc cggaaagcag
     5161 gcagctatat tttcgtggaa tttcacctgg agatgccgcc cgacaagaaa ctggaagaag
     5221 tgcacgctta ctgcgacagg attgaagagg agctgaaaaa acagatcagc aacctgcgta
     5281 ttaccataca tgccgaaccg gaggaatgat cccagggatg gcgtcccaaa ggatggcgtc
     5341 ccttttttcg cttttatttc acctcaaaaa cgtaccccag tccgtgccgg ttttcgatgc
     5401 tgaccagctt atcatcctgg aaatattttc gtaaccgcga tatgaataca tcgagactcc
     5461 ggccggagaa atagtcgtta ccaccccata ccgcctccag gatctgctcc cggctgagca
     5521 atttattccg gttccggctg aagtaactga tcagttcagc ttcgcggaat gtcagttttt
     5581 tcacaattcc tccacattcc agccgcaaca ggcccatgtc cagggtgcaa ctgccgatcc
     5641 ggatttcact ttcaggtgat tcagctttat ttaccctcga cctcctcagg aacacctcaa
     5701 tcttcaatgt taactcttca atgttaaatg gcttcaccag gtaatcgtcg gcgcccagca
     5761 acagcccttc tatcttgtct tccgtcagtg ttttagctga aatgaaaatg atcgggatct
     5821 cctcgttttc cttcctgaca aggcgtgcca gcgtaaaccc gtcgagtttt gggagcatga
     5881 catcaaacag gcataaatcg aaggattctg ttttgaagag acgatatgcc gattctccgt
     5941 ctttgcacaa aatgacctca tacccctttc tctccaggtt ctcggaggtg atgaacccga
     6001 gcgtttcgtc gtcttcgaca tacaatatcc ggcttctggt taccatacta ttctgttttg
     6061 ggtatggaaa tcgtcaccgt ggttccctgc cccggcgcac tctcaatttt cactttccag
     6121 ccatgcagat cgcacacctg cttgacataa ctcagcccca ggccggtccc cttgacattg
     6181 tgaatgttcc cgctggggac ccgaaaaaag ggcatgaata ccttcctgag atatttactg
     6241 tcaataccgg ttcccttatc ttccaattta ataaataccc gctttggatt ggccgacaag
     6301 gtaatgcaaa catccggagg cttgctggtg tattttaaag aattgtcgat gagattgata
     6361 agaattcctg aaaagtggta acgatcggcc aggatgaatg atgattgcgc tgccggtaaa
     6421 aacctcaggg taccgccggc atgttcggcg gatggtctga gttcctccag gacctctgtc
     6481 acgacatcct gcggatcgaa cttttgcttg ttcagcctga aaaacctgtt ttcggtttct
     6541 gcatgaacca gtattttatc gacttttttc tgaagcagga tgatctggtc tctcaggatc
     6601 gacgcgtatt tatcaaaccg tgccgtgtcg cctccgggcc cttcttccct gacaacatca
     6661 gaggcaagac caatggaggt cagcggcgta cggaactcat ggctcatgtt gttgatgaaa
     6721 tcccgctgaa tctccgaaag cttcttctgg cgaaggatga tcatcatact gtaaccgaaa
     6781 aataatatga ccatgaccag aatacctgta aaaaaatacc ataatcccat ctcccccaga
     6841 aggtagtttc gcattcccgg aaaatgaacc ccaaaataat agagcccttc ctcatacttt
     6901 ggccagtatt ccaacttgtt cttcacctcc tccgtctgat cgagcgaaac gtaatttcca
     6961 taaaccatgc ggtcgtcgtc acaatcatag atggcatatt cgaaatcgac atcgaggtgg
     7021 cggcgcatca gctctgtgcg taaataatgt tccagtaccc agggatcgat cctgttgttg
     7081 atatcgacaa taaaataatc gggactgtac tgatggatga tattgtcagg caaagtgctt
     7141 ttattgaact ggcttatgct ttccgccacc gattgcaatg aaatccggaa ggattgaacc
     7201 gtctgccttt cacgcaggtt aaaggcctgg taaaaaaaat aggcctgccc ggccaggatg
     7261 gctaaaatgg ccaggatacc caggccgatt acccattgta ccgaacggtt tgtcataaag
     7321 cgaaattact aaaattgagg ctgatggtat ttctattaac aattcattta ctattcatta
     7381 agaataatta tgactttgtt aaccaatcct tccggtctgt tcccctaatt ttggccgaag
     7441 attcttcaaa taaatttaaa tataatcaaa tgaaaaaaat tgcagcatta tttgttctgg
     7501 tattagtggg tgtgcagatc atttcagcac agacccaggc agtgcagcag cagcctgcca
     7561 aaacctattc atggaatgag acgacccatg atttcgggaa gattgtcgtg aacgatccgg
     7621 ctacggtaac ttttacggta aaaaacaacg gaacggcacc cttgctcatg accgcggcta
     7681 aaagctcgtg tggctgtacc gtggctgaat acacgaagga accgatcaaa ccgggtgaaa
     7741 cgggtattgt gaaagctacc tataacgcgg ccagggccgg ctcctttacc aaaacggtga
     7801 ccgttacttt tgaaggaatc tcttccggtg aactcctcac catcaaaggc gaggtaattg
     7861 caaagccgga gaactgatct tcacgatcat caccgttcca tccatatccg ggacatggac
     7921 tagcgtttcc ggaattttgc tgataaattc atctgtggcc cgcttaatcc cgggccattt
     7981 gtgcgtataa tcgtggatga aaatgacacc accgggcgac aaccggggat aaaaatactc
     8041 cagtccggct ttggtcgggt tgtacaggtc ggcatcgagg ttgacgaggg cgtaagcccc
     8101 ctccctttct ccctctccgc cagctggcgg aagggacggg gtgggggctg aaaaatcaaa
     8161 cctccccgca tgatacacaa acttcccttc ctcccccagg taacgcttca ccgcttccag
     8221 gctggtgtcg gcgaaattac gggtcgtata ggttgccgcc tctcccgttt cgttctcaag
     8281 atcttcttct ttaaagcctt caaaagtatc gtagagatga aaagtccgtg acgggtccat
     8341 catgtggatg atccttgcgc tttccccttt gtaaacgccc agttccgcga aatcgccggg
     8401 gatgccatcc cttttcagcc gctccacctg gaaccaccag ttgaaaaaac ggaccttatc
     8461 ggggtaatta cgctccagct tttcgagttc cttgctgacc cgcccattta taacggcata
     8521 ttcccactcc accggacggt agttcgaatc aaagaaaatc ccccaggcat accttaccag
     8581 gagaacaagt acgaccagga caaaagcggc ggaaataatg taaagaaagt tcaaagtgca
     8641 aagttcaaag tgcaaaactc aaagttcaga gtgcaaagtt agtgggcgga tgggttgatt
     8701 tgaagaattc ggaattaatt ttctaatttc ggtctggatt taaaagtgct attatttcct
     8761 gtgagcgggt atgagcagta aaaacgtcat tattaaaatc agtcagaaaa agatatggtt
     8821 caatgaatcc gtctcgatcg cggttgagtg ttcaaacctg cctgtcgatg atatcaaatt
     8881 tagccgggtg aatgagatct actggcttac agagcaagtc tcttttgaaa aggataccgg
     8941 cctgctcgaa gtgaatatcc aggattattt tctcagggac ttttcctctt ttaccgctca
     9001 aaatccaaaa tcgctggtta aatcaatccg gttccggaac ctcagcgata cagacagcct
     9061 gaagacggca ttacgctact attcgagcgg aatgaaccac ctcgaccagg cgatggaaga
     9121 ggctttccgg gagctttccg acaagccgga acccatcccg gagaaagtct ggaagaaaaa
     9181 tatcatgttt cggtcgtacc aggaaaaaga gaagctggaa gtgactttcg atgcctggtt
     9241 caaggatgcc gaattcaagc taggctatgt ggctttcgac aggaacatcc cggaactaaa
     9301 gcaaatgctg cggtttaagg ttgataatga ttttctcctg cctgagtttg agttcatcaa
     9361 aagctacttc gtgaaagcgc tcgggacccg gaaattcgac gtcaaagcca ctatattcct
     9421 ggaacacggg aaaccggtcg aaaccagcgc tgcatcggtc ctgatcaaga aaatcgatga
     9481 gaacctgatc gacagcatca agaatatccg gacggctgct ctattcaaac ctcccttcaa
     9541 ggtcaatgtc gacaaaagcc ttttttcggc tgatgaggtt ttcggtgagt ttggccccgg
     9601 tgaagccggc aatgttttca gccagaccga agaggagatg ctcgggcaga tcctggagaa
     9661 gtacaaggtt cggaaccgca agcagctcga attcctggcg ggcgtaaagc aggcggccgg
     9721 atcgaggctg aagttcacgc tgaatcccta tttcgggttt ctattcactg ttgaagggga
     9781 gaaaatgaac cacttcatct gggagctgct gaactcccat gctacttaca tctggagtct
     9841 cgacaagggc gacggcgcga tggccaggca gtaccggcgg gtggaagacg ccataaacca
     9901 ggtgcgcaac agcgggcgcg acaactacaa gcaggcctgg cgcgcctccc acatcgacca
     9961 tgacctggtg ttccatgtca tccaccacga cgatgccggc tcggctatga ttgatgggtt
    10021 tgtaaaatgg cggcaccggc tgggcgaggt gatgatttaa ggaattgggg gattggtgga
    10081 tttgacgaat ttgatgtttt taccgcgtag cggttaatcg cccaatcgcc caatcgccca
    10141 atctaaaacc tctccctcag ccgcctgtac ccttccgaac tcacgttcag tttctcgccg
    10201 gttttaagca gagcgacata gctgtctttt gtgtaaagtt ccagcgaccg gatttcgttg
    10261 atattgacaa tgtatttgcg gtggatgcgg atatactgct ctttcggcag gtgctcctcg
    10321 aagtacttca tggtcttctc tttcagatgg tcgcccagct cggaatggat catgacgtaa
    10381 tcatcggccg attcgatgta cctgatctta tccaccggta taacggtaat tttggagccg
    10441 agccgggtga tgacccggtc gagggtctgc cgggtgtcgt cgaggtgctt caaaagggat
    10501 ggcgtccctt gggacgccat cccctggggt gtcgtcccta accggagctt cgccttttca
    10561 tacgcctgca cgaaacgctc cctcgaaaat ggctttagca gataatccac cgcattcagc
    10621 tcgaacgccc ggatagcata ctggtcaaaa gccgtagtga atatgatggc cggcttctca
    10681 tccagcacct cgagtaactc aaaaccatca atcttcggca tctggatatc caggaacaca
    10741 agatcaggcc tgagttcctg tatcgccttc agggcctcga aaccgttgga gcattcacaa
    10801 agcagctccg catcggctaa cccggccagg tatttcttta ccaattcccg cgccagatcc
    10861 tcatcatcca caatgataac tttaatcttt tcttgcatat tccggaattc tgatagtgac
    10921 ttcaaaataa ttctccgttt gattgatctt catcaggctg ccggttccat aaagattcag
    10981 taacctgccc cggatgttgc gcaacccggt ccccgtcccc ttttttgcca tggtaccgaa
    11041 accgtaggta ttgccaatcc tgatctccag ctcgctttcc aggatccggg cccccagccg
    11101 gattacagtc gtttcttcag attcgtaaac accatatttg acggcgtttt caaccagtgg
    11161 ttgcaggatc atgaccggta caatccagct catcgcttcg ggctgtattt ccttctcaaa
    11221 tgccaggcgg ctgccgaaac ggaccttctc gatatccagg taaagttcgg cattgtagag
    11281 ctccctttcc agcgtaccgg tcatattgtc cggaaagttc agcgaatagc gcataaactc
    11341 ggataacttg ataaccattt cctgtgcctt tcccggatcg gtcatcgtga gggcgctgat
    11401 ggagttcagg ctgttaaata aaaaatgagg cctgatctgc gaccgcagaa gattcagctc
    11461 cgcctctttc agcaatccag tgagccttgc ttcccgctgg ctcttttctt ttaactccct
    11521 gaaattgatc agcaggtaaa aaatggaaac cagcagggcg taaaacatca atcccgccaa
    11581 tatcctcaaa aagacggtat tctttagaaa agattgatag gcctgatcct cagggaataa
    11641 tgtattcaga atcaggtggc ccgccatcat ccatacaaaa atggccacag ctgcgccggt
    11701 tatatggtat aacgccagct cggctttggt ttttgtctgc aaattggagt acctgaccat
    11761 aaaccacagc ccgctgccga tcaccgtaaa aagcccgttg aaaaccaggc tgtcggcaaa
    11821 agcatggacc gaccggaggc cataaccgaa atacaaaagg gaaaagtgga tgacgcccag
    11881 cgtcacccac atgatcaggt aaacaatctc caggtgattt tttattcctc ttgccatatc
    11941 aatccagcat ccgggatcag tagctcttta tttcccctcc gccaaaaatg gctacccctt
    12001 ttacgataac cgtcttgttg aaatcgacct gccccctgat ccgcttatcg ctgtatccac
    12061 caaagatatt aaaaacttcc agtttgatgt tccagtcgtt cggaacaacc agcgtcgatc
    12121 cgccgaaaat agccacaatc tcaatctcca cgttgccggg tgccagctcc actttggtca
    12181 tgtcgagggt ggatcctccg aaaaccgaga ccaccttggc ccctttgagc gattgcgaat
    12241 tgatgaaccg ctcccggccg ccgaaaacgg ctacctcttc tacgaagtca tctgtgatgg
    12301 tgccggagga aaacccggaa gaatcggtcc aggttttgtt atgtgaatag gaccttttgc
    12361 agagcctgga tgagccgaaa ataaggatca gcccgataat gatcaacagg gcgggccaaa
    12421 aagtgctccg gtaattttca atatcaaaga tgaccgggat catgaagaat ccgccgatcg
    12481 cgatcaggat ccagcctacg cggcgggagg agtcgttcac gatattcacc aggccgatgg
    12541 cgatcagcaa catctgccag gagaaaataa cgtcccagat ccgggcgcct tcaaagggtt
    12601 ccagatttct gaaaaaaaga gcgatcccga gtagaatgac caggacgccg aataccagtg
    12661 atttgccttt aaggccatgc cttacggcag gttgtgtgtt gttttccatt ttcgtatgat
    12721 tttgaatgtt gaacattgtt ttcacaaatt tgatggggca aaaataatac ggcgacaatc
    12781 gccagagaac ctgaaatcgg cgaagaccgg gccggaatcg gtaaacaagg gaaaatagtg
    12841 gtacttttga gcattgaagg ttgaagattg taggttgaag tttgcaggtt gaagattgaa
    12901 tgacaggaaa tcatcataac ggagcggaaa tcatgacctt gtcgcagctc gcggcaggca
    12961 ttacggtggt tttggaagaa aacttccggt cgccgttatg gatcaaagcc gagatagcca
    13021 agctgaatta ctacccggcc agcggtcatt gctatccttc actggtggaa aaggaaaagg
    13081 ggagcgtaaa ggccgaactg agggggacga tctgggctaa cgactatgta aggatcaata
    13141 gtaatttcct gaaagtaata cgcgaaccgc tgaaggaagg tatacagata ctcttccggg
    13201 cctatgtcag gtttcatgct gtgtacgggc tttctctcca gatcatcgac attgactcgt
    13261 cgtataccct gggcgagatg gcccgggaaa agatggagac cctggcccgg ctcaaaggcg
    13321 aaggcctgtt cgaccggaac aggcagcttg aattacccgc tctgccgaag agaattgccg
    13381 ttatttcggt aaaatcaagc aagggcttca gcgatttcat aaatatcatc gaaaataacc
    13441 catggaaata tgggatcagt tgtgtcctct tccccgcgct gctccagggg gacaaagcgg
    13501 tagagtcaat ccggagccag atggaactta tcggccggta cgcacacctg ttcgatgcgg
    13561 ttgccattat caggggcggt ggaggtgagg tggggctgaa ttgctacgac aactacatgc
    13621 tggccaggga tgtagctctt tcccagctgc cggtgctgac cggaatcggg cattccacca
    13681 atgaaaccgt agtggaaatg atcgccggtt acaacaagat cacccccacc gatgtcgctt
    13741 atttcattat tcagcagttc cataattttt cgggaaggtt gaacgaactc catgagaatc
    13801 tggcaacaat tgcaaatgat attttggaag aggaaaaaaa gaggtgggaa gtattctgcc
    13861 tggagctagc cacgcaaaca gaaaatctcg tcagaaatga aaagcgggcg atattggagt
    13921 tatcggggtc ggtgatcgat ggaacgggga tcttgattac cggccagaaa catctcctca
    13981 acctggccgg attgaaactg tcgtacaaac cggcaggact gttgcgggag agcaggaccg
    14041 gtctcgaaaa ccattgggag cgtttttttc tgcatgtgaa acaactgttg aagcaaaaaa
    14101 acagcgaaat tgaaaaccta aaccacaaaa taaaccttct gaatccggtc catgtgctga
    14161 aaagaggtta tagcatcaca tacctcaacg gaaaagcact aattgactgt tcagaggcaa
    14221 aagcggggga taacttaaca acccggcttt ataaagggga aatttcgagt gaagtcaaat
    14281 cattaaagga ttaatcaaat ggagaagcaa attacttata ccgaggcgat gaaagaactt
    14341 gaactgatcg tcggtgaaat agaagaaggg gagatcacga tcgattcgct ttccgaaaaa
    14401 gtaaagcggg catcggagct gatcaggatt tgcaaggaga agctgacggc gacggaggag
    14461 gatgtcagca agattctgga aggattgaag gaaacatgat gatgacccct atttttaggt
    14521 cttgatttat tcgcataact tcgtgttttc ttcgtgtaac ttattgtatt agaatcatat
    14581 atatttattt tcacgaagat ccacgaagga gtcacgaagg atcacgaagg ttataaaaca
    14641 tatcttttta taccactctt tagaattgtc acattaaagt tgatcaataa tcctaacctg
    14701 atttctgcaa acctcatata agtcagaatc tgggcttcat ggacaggcgc taataattct
    14761 actgatttta attcaatgat tatcttctgc tcaacaagca aatcaagtcg ataaccacaa
    14821 tcaagtttaa tatccttata aattatagga attggttttt gtctgtggat cattagtcca
    14881 gttgaggtca actcgtaagc gaggcattct tcatatgctg attccagcat tcccggacca
    14941 aggattttat gtacctgcaa agcacagcct attatcttgc gggataaatc atcaaattcc
    15001 atttgcaaca ttttcttcgt gtaacttcgt gttttcttat atgtttgttt ttttattcaa
    15061 acaaatattg ttatgcttgc aacaaaggg
//
LOCUS       DAOQXS010000006        14966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4456_length_14966_cov_4.387298, whole genome shotgun sequence.
ACCESSION   DAOQXS010000006 DAOQXS010000000
VERSION     DAOQXS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4456_length_14966_cov_4.387298"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3077)
                     /locus_tag="PKM34_00410"
     CDS             complement(<1..3077)
                     /locus_tag="PKM34_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNQ82067.1"
                     /translation="MYWIYKNSWDTTFGDKGYGKEPFWIGTGAPSYIPSIGSSVYAIN
                     YDDTCSYPPITVICHRNCVTPNFTYYVHDYDKDKDGFFNWGIGQRPDTYPCSQEEDSN
                     DDDNRIGPYDDNYSGLAIMPDIKVKQGTSQWGGAFENGAVINISVDTLYDSTFVFQIG
                     NAGTAQLNLVQYQLSDSGVVTIDYQYPEGQFSLVDLPHKFVCMSDTSTFKIKMFHGAA
                     AGALAHVHIYLDEPDMDSIFEFTIVYNGCETSSGYDSITSEIEWNDTCRAQCRDLHIM
                     RTGELTVLGMVRLSPEADIIVEPGGKLIVDGGCLTRACNSQLWKGIQVWGDSSLSQYP
                     DTNQGFVSVVNGGQIEYAQGGIFAGRADDGDTLYAYSGGTIYAEKAVFLNNVIDVEFL
                     PFINRTPGNQEMENLSHFRDCIFRTHDPDGLIEKVSAHAILDGVNGVDFYTSTFEMEE
                     TEGNPFETDGKGIGIWATDAEFNVRGMCDTNTIPCIHYDSCYFKNLRYGILATNSGGN
                     NYVWVTETVFDNNIAGIYLSGYHQPSVYSSRFYTCTEQGPPGDLDDAFLGGLYLEGST
                     GYQVEENYFAGPLTTMPDSGSIATIGIYIKDSGDDENEIYNNYFTGLDAGIIAEGVNR
                     GDKTGLCLKCNDYRTCLNDMLVIEGDSNSRYLGIKEYQGTDDTLSSALAGNTFTSDVQ
                     GLQAVDNGGNKKYYWSYFNDADHINYYHHGQHPFLVTYPSDSNYTDNTITLNNKQTPY
                     LKEEACPSGISNPELKSIEDPRLSINEADQQLQLLENKFDSLVDGGDTEGLDFEIMTS
                     MPDEALELQQQLLSGSPYLSDTVMKQAIYKENVLPNAMIRDILSVNPQSAKSGEILDA
                     LDSRYDPMPDYMMANIMQGLHQIGALESLESKIGYWQNYRSRAVNRLVREYLSDSTLF
                     NTEDSLIGLYQDENTLQSKYRLAFSYWDDENMEQAQNALSAIPGEFNLSSQEQTVYQN
                     YLDFFNILQLMSDSSLHARQLDFTAVQQLVNIMNTGLPLVSNYARALLIKGRH"
     gene            complement(4786..5748)
                     /locus_tag="PKM34_00415"
     CDS             complement(4786..5748)
                     /locus_tag="PKM34_00415"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNQ82068.1"
                     /translation="MFRNSVIFISIVALLASCKPEVKNAFVIDGTVDTTVSGYVLLQK
                     RVEGPMVTVDSVQLTEKGTFRFEGTVGYPEVYYLNIPSTRSSVPFFIEPAPIIVNIIT
                     KDIDKTKITGSKTQTEYDAYLDKLDLFSKKIRDNYQLYNMAQEAGETEKVKYFDSLIT
                     VLDNERTQFSKDYVLQNKSSFISPYIIYRNSYAYELEELETALGGFDTALSHSVYTGF
                     AKDYLATLKRVAIGKPYVPFSMPDSAGTAVALSDQVGKGYLLVDFWASWCAPCREENP
                     NLVKLYSEYHERGFDIFGVSFDTKRDRWLQAIEDDGLTWGTFRI"
     gene            complement(6008..6523)
                     /gene="mreD"
                     /locus_tag="PKM34_00420"
     CDS             complement(6008..6523)
                     /gene="mreD"
                     /locus_tag="PKM34_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNQ82069.1"
                     /translation="MNKKLIGNIARFVVLALAQVFIFNKVMVSGYVNPMVYVLFILML
                     PYDIKGFQLLLLAFLMGLTIDLFMHTPGMHALASVTLAFLRPGVIRLVGKKDDVEPWQ
                     YPNVRDSGAAWFLAYTVILVFLHHLMIFYLEVFRLDEFLRTFVKVLINTALSTLIIMM
                     LQYLFYSRKVE"
     gene            complement(6516..7337)
                     /gene="mreC"
                     /locus_tag="PKM34_00425"
     CDS             complement(6516..7337)
                     /gene="mreC"
                     /locus_tag="PKM34_00425"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNQ82070.1"
                     /translation="MRNLLAFISRYQFLFFYLIFLVISFTLLYNNNYYQRSKVVGVTN
                     GITGKFNTYYENMTSFIDLKRSNDELAKENARLRALLNLKTRIIPDSLVRLENDLTEK
                     YISARIISNSTQYRNNFFMIDKGYTSGIKKDMGVIAGNGVVGIIIDVSEHYSSGISVL
                     HKDSRISGRIKKNDQLVNVSWDGVNYRLGDISHIPTHVSLLPGDTILTSGNSRIFPEG
                     LMIGTVEYVDDEMENMFKKGKVRFTVDYNKISYVYVVDNPTRGELNRLQTKTPNE"
     gene            complement(7339..8361)
                     /locus_tag="PKM34_00430"
     CDS             complement(7339..8361)
                     /locus_tag="PKM34_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ82071.1"
                     /translation="MGLFSFLTKEIAIDLGTANTIIIYNDKVVVDEPSIVALERNTGK
                     VIAVGKKAMMMHGKTHENIRTIRPLRDGVIADFKAAEIMIREMIKMIGFKNNVFPPAL
                     KMVICIPSGITEVEERAVKDSAEQAGAKEVRLIHEPMAAAIGIGIDVLEPTGNMIIDI
                     GGGTSEIAVIALGGIVNNKSIRIAGDDFNADIEEYMRKQHNINIGERTAERIKIEVGA
                     ALTDIDNPPDDFPVHGRDMLTGIPKEITVNYAEIAHSLDKSISKIEAAVLNALEMTPP
                     ELSADIYRTGIYLAGGGSLLRGLDKRLHLKTKLPVHMAEDPLRAVARGTGIALKNFDK
                     FTFLIK"
     gene            complement(8393..9874)
                     /gene="purH"
                     /locus_tag="PKM34_00435"
     CDS             complement(8393..9874)
                     /gene="purH"
                     /locus_tag="PKM34_00435"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ82072.1"
                     /translation="MERKIRSALISVYYKTGLEDILHELRRNGVTIYSTGGTYDFITG
                     LGIPAITVESLTAYPSIFGGRVKTLHPKVFGGILHRRENEEDVKQAKNYNIPPIDLVV
                     VDLYPFRETVARTTDEDEIIEKIDIGGISLIRAAAKNYEDVLIISSVNQYPDLLKILK
                     DQDGVTTLEERRRFAGMAFGVTSSYDMAILGHFQPPVKLRYGENPHQQGTFHGDPGRV
                     FTQLHGKELSYNNLGDIDAAIALIKEFDEPTFAILKHNNACGCASRPVLADAWRKALA
                     GDPLSAYGGVLITNREVDEATAEEIDKLFFEVILAPSYKKSALEKLQSKKNRIILRQG
                     SYDFPERQFRSLLNGIIEQDKDLKTETRDDLRPVTERVPTERETDDLLFANKIVKHTR
                     SNAIVLAKDRQLIGSGVGQTSRVDALKQAIAKAGEFGFDLQGAVLASDAFFPFADSVE
                     IAHKAGITAVIQPGGSVRDQDSVDFCRNHGMAMVFTGTRHFKH"
     gene            9976..11226
                     /locus_tag="PKM34_00440"
     CDS             9976..11226
                     /locus_tag="PKM34_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82073.1"
                     /translation="MKIFLKLLMESYLFAVTEIIRNKVRTLLSLLGITIGIFSIISVF
                     TIFDSMESSIRSSINSLGNDVLFVQKWPWAMGGDYPWWKYISRPEASLTDLKEIQRRS
                     QASETSSYLSGLSETIKYREKSIQNANVIMVAENYDRVMTVDITEGRFISPLEFSSGR
                     PVVVIGSDIAENFLSSTTASALGKKIRIFGMNVEVIGVMKKQGNNMFGNSSDDQLIVP
                     VNFARNHVDMRYAQGAIIVKARTGVSLDQLRDELQGIMRAVRKLKPKAEDNFAINQTN
                     VISKGFDQLFGVIGVVGWIIGGFSLLVGGFGIANIMFVSVKERTRIIGIQKSMGAKNY
                     FILLQFLFEAVFLSLLGGLLGLLIVFLLTFVISRSFDMELVLTVGNIILGITVSALIG
                     FVSGLVPAWNASRLDPVEAMRSTF"
     gene            11334..13088
                     /gene="recJ"
                     /locus_tag="PKM34_00445"
     CDS             11334..13088
                     /gene="recJ"
                     /locus_tag="PKM34_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNQ82074.1"
                     /translation="MEKRWVLKPQGNKDLVRHLSKVLNINENLANLLAQRGVASYDGA
                     KAYFRPQLEHLHDPFLMKDMHKAVERIEKALKKGEKILVYGDYDVDGTTAVALVYTFL
                     KKLHEKVDFYVPDRYSEGYGISFKGIDFAAENGFSLIIALDCGIKAVEKVAYATGKKV
                     DFIICDHHRPGDSLPEAIAILDPKRDDCTYPYKELAGCGIGFKLIQAFAWKNNIAFSS
                     LEEYLDLVVVSIASDIVPITGENRVLAYYGLKRINSRPRAGLEAILNVSNKYSKTNGE
                     GPDRQYIRELTINDLVFIVGPRINAAGRIKSARTSVELLITKDPAEAFELGNQINKQN
                     SDRKTLDTQATHHALEIIHSDPKFLKRRSTVLYHPEWHKGVIGIVASRLIEAYYRPTI
                     VLTHSEGMITGSARSIKGFDIYDAVDSCGDLLEHFGGHMYAAGLSLLPENFDKFKERF
                     EEYVSTHLTEELAVPEIEIDSKLNLQDVQRKFYDILRQFAPFGPGNMSPVFQTDNVID
                     AGKAKIVGNNHLKLEVFQRDVRSEPISAIAFGQGEENFEHIRDGNPFDICYHIEENEW
                     NGRKSFQLNIKDIKWSAG"
     gene            complement(13090..13614)
                     /locus_tag="PKM34_00450"
     CDS             complement(13090..13614)
                     /locus_tag="PKM34_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ82075.1"
                     /translation="MKTSNWFVFLINGLIAILFGVFVLMVSQDMMLKYVRYFGIFILV
                     CGLVLFYFSYRNKKANKSYVLNMVMSIFAFLIGLLIAINPGKSLDLFMTLIGIWAALM
                     GLLQIILSVRMRKKVTNHYLFSINGVITLVFGLLLFYSQMGTDGTLSKVIGVLAVVAG
                     VFLIYLSTKVKAIK"
     gene            complement(13699..14334)
                     /locus_tag="PKM34_00455"
     CDS             complement(13699..14334)
                     /locus_tag="PKM34_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNQ82076.1"
                     /translation="MQTIIEGILLGLTLSIFFGFGPALVAEIQTSILRGFWAAVLLAF
                     GVFLSDAVLVALGFLGAVQIFESNKTILGLVGGIVLIIFGIVTYRRPVLINVDTSDPE
                     YKKQEPFFTTYILKGFFINFTNPFIWIFWMGIVVGFTANYKADMLMLVSFFASALGTV
                     LLFDVLKIFSAYKVKKYLQTHNIVWINRIAGVGLVLFGIYLVVKTYLLIGV"
     gene            complement(14339..>14966)
                     /locus_tag="PKM34_00460"
     CDS             complement(14339..>14966)
                     /locus_tag="PKM34_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNQ82077.1"
                     /translation="DGTITDRRGSLNFDDEGNMVDKPVALTIDQVEEGKEYALLLSTC
                     AGAWRYLIGDTIKFTSIMHSEIVITGRTKHFLSITGEHLSVENMSRAVKMLCDEMNVE
                     IREFTVAGIKHDTMWAHKWYLGTDDPLNAAVAREKIDEALKILNDDYRVERIAAIKDV
                     FVEVLPSAAFSTWMKIHGKEGGANKFPRVLKKDQPAAWEQYLKEYNFK"
BASE COUNT         3743 a         3825 c         3591 g         3807 t
ORIGIN      
        1 atatgccttc ctttgattaa caatgcccgc gcataattgc tgaccaacgg cagaccggta
       61 ttcatgatgt ttaccagttg ctgaacggca gtgaaatcga gctgccgggc atgaagactg
      121 ctatcgctca tcaattggag aatattgaaa aagtcaagat aattctggta aacggtctgt
      181 tcctgggaag ataaattgaa ttcaccagga attgcagata acgcgttttg agcctgttcc
      241 atattttcat catcccaata agaaaaagca aggcgatatt ttgactgaag tgtgttttca
      301 tcctggtata aaccaatcag gctgtcttca gtattgaata aagtcgagtc ggacaaatat
      361 tctctgacca gcctgttaac tgctcttgac cggtaattct gccagtatcc gatcttcgat
      421 tcaaggcttt ccagggcacc gatctgatgc aacccttgca tgatattggc catcatgtaa
      481 tctggcatgg gatcgtaccg ggagtctaat gcatcaagga tttcaccgga ttttgccgac
      541 tgcggattta cagataagat atccctgatc atcgcattgg gaaggacatt ctctttataa
      601 atagcctgct tcattaccgt atccgagaga tagggcgacc cggaaagcaa ctgctgctga
      661 agttccagcg cctcatccgg catactggtc ataatctcga aatccagccc ttccgtatct
      721 cctccatcaa ccaatgaatc aaatttattt tcaagtaatt gcaattgctg atccgcctcg
      781 ttgattgata atcgtggatc ttcaatactt ttaagttctg gatttgatat tcctgatgga
      841 caggcttcct ctttcagata aggtgtttgt ttattattca atgtaattgt attatctgta
      901 taattactat cagaaggata ggttacaaga aaaggatgtt gtccatgatg gtaataatta
      961 atatggtcag cgtcattaaa atagctccag tagtattttt tatttccgcc attatccact
     1021 gcttgcagtc cctgaacatc cgatgtaaaa gtattccctg ccagtgccga acttaacgta
     1081 tcgtcagtgc cctggtattc tttgattcca aggtacctgc tgttgctgtc accttcgatc
     1141 accagcatat cgttcaggca ggtgcggtaa tcgttgcatt tcaggcaaag gccggtttta
     1201 tcgccccgat ttaccccttc cgcgataata cctgcatcca gccctgtgaa atagttgtta
     1261 tatatctcgt tttcatcgtc gcctgaatcc ttgatataaa tgccaatcgt ggcaatcgaa
     1321 ccgctgtcgg gcattgtggt caatggcccg gcaaaataat tctcctcaac ctggtacccg
     1381 gtggagcctt cgaggtacaa accgccaagg aatgcgtcat ccaagtcccc gggaggcccc
     1441 tgttccgtgc aggtgtaaaa acgggaagag taaacagaag gctgatgata acccgataaa
     1501 taaattccag cgatgttgtt gtcgaaaacc gtctcggtca cccacacata gttatttcct
     1561 cccgaattgg tggccaaaat gccatagcgc aggtttttaa aatagcatga atcgtaatga
     1621 atacaaggaa tggtgttcgt atcgcacatg ccccggacgt taaactccgc gtccgttgcc
     1681 catatcccga tcccttttcc atcggtttca aacggattgc cctcggtttc ttccatttcg
     1741 aaggtgcttg tgtaaaaatc caccccgttc accccatcca ggatggcgtg tgcactgact
     1801 ttctcaatga gaccgtcagg atcatgagtt cggaagatac aatccctgaa atggctcagg
     1861 ttctccattt cctggttgcc cggggtcctg ttgataaacg gaagaaactc aacgtcgatg
     1921 acattattca ggaaaaccgc tttttcggca tagatcgtac cgcctgagta ggcgtaaagc
     1981 gtatcaccgt catccgcccg cccggcaaaa atgccaccct gggcatactc gatttgcccg
     2041 ccattgacca cgctgacaaa tccctggttg gtatcgggat attgtgacag cgaactatct
     2101 ccccatacct ggatgccttt ccataactgg ctgttacagg ctcgggtcag gcatccgcca
     2161 tcgacgatca gttttcctcc cggttcaacg ataatatcag cttcagggct aagccttacc
     2221 attcccaaaa cggtaagctc accagttctc ataatgtgaa ggtctctgca ttgcgcacgg
     2281 caggtgtcgt tccattcaat ttcagaagtg atggaatcat aacctgatga ggtttcacag
     2341 ccattataga caattgtaaa ttcaaagata gaatccatat cgggttcatc gagataaata
     2401 tgcacatgag cgagcgctcc ggccgcggcg ccatggaaca ttttgatttt gaaggtagag
     2461 gtatcgctca tacaaacgaa tttatgaggc aagtcgacaa gactaaactg tccttcaggg
     2521 tactgataat caatggtaac aacgccacta tccgatagtt ggtattgaac aagatttagc
     2581 tgtgctgttc cagcattacc gatctggaat acaaatgttg aatcatacag cgtatcaacg
     2641 ctgatattta ttaccgcacc attttcaaac gctcctcccc actgactcgt accttgtttt
     2701 accttaatat caggcatgat agctaagccc gaataattat catcataagg cccaatccta
     2761 ttatcatcat cattactgtc ttcctcctgg ctgcacgggt aagtatctgg tctttgtcct
     2821 attccccagt taaaaaatcc atctttatcc ttgtcataat catggacata ataggtaaaa
     2881 tttggtgtaa cacaattgcg atgacaaata accgtaatcg gaggatagga gcaggtatca
     2941 tcataattaa tggcatatac agaacttcca atacttggta tatatgaagg cgctccggta
     3001 ccaatccaga atggttcttt tccataaccc ttatcaccaa aggttgtatc ccaactattt
     3061 ttatatatcc agtacaaggt gtcattttta gtttcaaaac caatcaaaga aactgcatgt
     3121 tgagcagaat ctggccctgg ccaattttta agagttacag acaaaggccc atcagtcatt
     3181 atttttactt ttaaagaatc tgccctttga atagaatcgc cctgtgttat ttgatatctt
     3241 ttatacgacc atatttgagc ggtcattgaa ggggtgtcac atttttgctg ggcaactatt
     3301 tcacacgaag ctcctgtgtc ttcgcaaatc ggcgattcat acggatagca atctgaatca
     3361 actatagcaa tatctttaaa aaaatcatat atcagggaaa tgccttttcc ttcatcacaa
     3421 ttacatccta ccgttgtaat tccactttta tatttgctgc aattaaatgc atgacgttct
     3481 gaaaaccttg tatcttcaat tgaatcaaat tgataatttg cataaaggtt gattgctgct
     3541 tccagggaag caacactagc aaaagctgca caagcagtac aagttttctg atcctttact
     3601 cttgtcatcc atccgttagt agggtcttgt gaatctccac cataatatgg agatgaagaa
     3661 agatgggcat tgtgtttttg acgccaatca aatgaaggaa tgatagtata atcggtgtat
     3721 tttggaatgt ttgcgtgcct tatcgattta aaaatcccgc cggagtaata gtctaaaccc
     3781 agaagattaa acttttcatc aaatctttgc atcttatctt tataaaacat ctttacaaga
     3841 ttggtggtat cagccgtcca gttccatcct ctggcctgga cattgatatt catttgctga
     3901 actttctcca ggtctttgct tcgctttgcc tgatattgta agagggcagg attttcacct
     3961 ggaatagtat ttacggtaaa ggtcgagata tatagttcag catcaatgat ttgaatcgtt
     4021 atcgaaacag gaattgtttc atccagagaa caagtttcat cacattcatc ccctaaatta
     4081 aattctttag tgctggcgat caatggatag gattcataaa tcatccagtt attaccgttt
     4141 atatctgata agattatcct taccaagctc gtatcacttt ttagattcac tgaaccgctg
     4201 acagatatgc cataaatagt atcttcgggc gtgaaaggct gtatttcagc atctgatgtg
     4261 aaggtttggt atatctcaat cgtctgggag ttcacgctca gggtgttagt aaaaaataat
     4321 accataagca atcctgctat ggcgcctgat ttgaaaacat ttcttttcat acttttaaga
     4381 ataatcggtt aataattggg gggagacatt ggtgcgataa agatatgtta ttccggaagg
     4441 aaaagcaagg gatttgaaat attttctgct acaatattgg atcacctacg gtgaagctgc
     4501 agggctccgg gttccaaagt tccactgttc cactgttcca ataaatgacc accaatgacg
     4561 cgagtgcagc gagctaatga ccaagcaagc gggcacgcgt ccatgttcaa tcgtccatga
     4621 atcacttcct ccccttcacc ggctccggcc agatctccgc gagcctggcc cgcaggtcgt
     4681 cgccacgcag gttctttgca atgatcacgc cgttgctgtc gagcaatacg tttgacggga
     4741 tagagcggat gccgtaaagc ttgcccgccg cgttctccca gcctttcaga tccgaaacgt
     4801 gccccaggtc aatccgtcgt cttcaatggc ctgcagccag cggtcgcgct tcgtgtcgaa
     4861 tgaaacgccg aaaatatcaa accctctctc atggtattcg ctgtaaagct tcaccaggtt
     4921 cgggttctcc tcgcggcacg gagcacacca cgatgcccag aaatcgacca gaaggtagcc
     4981 tttccctacc tggtcggata atgcaaccgc cgttcccgcc gaatcgggca tagagaacgg
     5041 cacatatggc ttcccgatgg ccacccgctt cagcgtggcc aggtaatcct tcgcaaaacc
     5101 ggtgtacacc gaatgcgaca gcgccgtgtc gaacccgccc agggccgtct ccagctcttc
     5161 cagctcataa gcgtacgaat tccggtaaat aatgtacggg ctgatgaatg aggatttgtt
     5221 ctgtaataca taatctttcg agaattgcgt ccgctcgtta tcgagaaccg tgatcagcga
     5281 atcgaaatat ttcaccttct ctgtctcacc cgcctcctgg gccatgttgt agagctggta
     5341 attgtccctg atctttttgc tgaacagatc cagcttatcc aggtatgcat cgtactccgt
     5401 ctgcgtcttc gatccggtga tcttcgtctt gtcgatatct ttcgtgatga tgtttacgat
     5461 gatcggcgca ggctcgatga aaaacggcac cgacgaccgg gtcgacggga tgttcagata
     5521 ataaacctcg gggtaaccga ccgtcccttc aaaccggaac gtgccctttt cggtgagctg
     5581 aacggaatcg accgtcacca tcgggccttc aacccttttc tgcagcaaaa cgtaaccgga
     5641 taccgtggta tcgacagtac cgtcgatgac aaaggcattt ttgacctccg gcttgcagga
     5701 ggccaggagg gcaacaatcg aaatgaagat tacagagttt ctaaacataa aagaaatatt
     5761 aatttattat acaaattttg ttgctccaac gactaaagcc gttggttgcc tgtgattttg
     5821 ctccaacgac taaagccgtt ggttccggaa ttgattgaac cgacggcttc agtcgtcgga
     5881 ctacctaaaa aaagtgaata tatggacttt tgagacatcc ttacaacaaa cctaaaaagc
     5941 aaccattcaa cttttaatac agcttcacga aattcgttcc aacaataaca aaactaagaa
     6001 caatctctta ctccaccttc cgggagtaaa acaaatattg cagcatcatg atgatcagag
     6061 tgctcaatgc cgtgtttatc aacaccttga caaaggtacg caaaaattcg tccagcctga
     6121 aaacctccag gtaaaagatc atcaggtggt gcaggaaaac caggatcacc gtgtaggcca
     6181 ggaaccacgc cgcccccgag tcacgtacat tcggatattg ccacggctcc acatcgtctt
     6241 ttttcccaac cagccggatc accccgggcc tgaggaaggc aagggtaacc gacgcaagcg
     6301 cgtgcatccc cggcgtgtgc atgaaaagat cgatcgtcag gcccatcagg aaagccagga
     6361 gcaacaactg aaatcccttg atgtcgtaag ggagcatgag gatgaacaga acgtacacca
     6421 tcgggttgac atacccgctg accatcacct tgttgaagat gaagacctgc gccagcgcca
     6481 gcacaacgaa acgggcgata tttccgatca gcttcttatt cattcggcgt cttggtttgc
     6541 aggcggttca gctctccccg cgtcggattg tcgaccacgt aaacataact gatcttgttg
     6601 taatcgacgg taaacctgac ttttcccttc ttgaacatat tctccatctc gtcgtcaaca
     6661 tattccactg ttccgatcat cagcccttcg gggaaaatcc gcgaattgcc gctggtcagg
     6721 atcgtgtcgc cgggcagaag gctcacgtgt gtcggaatgt gcgaaatgtc gcccagccgg
     6781 tagtttacgc cgtcccagga gacattcaca agctggtcgt tctttttgat ccttccgctg
     6841 atccgggaat ctttgtggag gaccgagata cccgagctgt agtgctccga cacatcgatg
     6901 atgatcccca caacgccgtt gccggcaatc acccccatgt cttttttgat cccgctggtg
     6961 tagcctttgt cgatcatgaa aaagttgttg cggtattggg tggagttgct gatgatacgg
     7021 gcgctgatat atttttcggt caggtcgttt tccagccgca ccagcgaatc ggggatgatc
     7081 cgcgttttca ggttcagcag ggcccggagc cgggcattct ctttcgccag ctcgtcgttc
     7141 gatcttttca ggtcgatgaa gctggtcata ttttcatagt aggtgttgaa ctttccggtg
     7201 atgccattgg tgacgccgac gaccttcgaa cgctggtaat agttgttgtt gtaaaggagc
     7261 gtgaaggaga taaccaggaa gatcaggtaa aaaaataaga actggtacct ggaaatgaat
     7321 gccagcaggt ttcgcatctt actttatgag gaatgtgaat ttgtcgaagt tcttcaacgc
     7381 gatgcctgtt ccccttgcta cggcccgcag cggatcttcc gccatatgga ccggcagctt
     7441 ggttttgagg tggaggcgct tgtcgagccc gcggagcagc gaaccgccgc cggcgagata
     7501 gatgcctgtc cggtaaatgt cggccgaaag ttccggcggg gtcatctcga gggcgttgag
     7561 caccgccgct tcgatcttgg agatcgattt gtcgagcgag tgggcgatct cggcgtagtt
     7621 caccgtgatc tccttcggga tgccggtcag catgtcgcgg ccgtgtaccg ggaaatcgtc
     7681 gggcggattg tcgatatcgg tcagcgcggc gccgacttcg atcttgatcc gctcggccgt
     7741 ccgctccccg atgttgatgt tgtgctgttt gcgcatgtac tcttcgatat cggcgttgaa
     7801 gtcgtcgccg gcgatgcgga tcgacttgtt gttcacgatc ccgccgaggg cgatgaccgc
     7861 gatctcgctg gtaccgccgc caatgtcgat gatcatgttg ccggtgggct ccagcacgtc
     7921 gatcccgatc ccgatcgccg ccgccatggg ctcgtggatc agcctgacct ccttggcccc
     7981 cgcctgctcg gcggagtctt taacggcccg ttcctccact tcggtgatcc ccgacgggat
     8041 gcagatgacc atcttcagcg ccggagggaa cacgttgttc ttgaagccga tcatcttgat
     8101 catttcccgg atcatgatct cggccgcctt gaagtcggcg atcacgccgt cgcgtaacgg
     8161 cctgatcgtc cggatgttct cgtgcgtctt gccatgcatc atcatggctt ttttcccgac
     8221 agcgatcact ttgcctgtgt tccgctcaag ggcaacgatc gagggctcgt caacgacgac
     8281 cttatcgttg taaatgatga tggtgttggc agtaccgagg tcgatggcga tttctttggt
     8341 gagaaatgaa aagagtccca ttcgttctat ctgttatgcg ctgtaaatac tcctaatgtt
     8401 taaaatgccg cgtacccgtg aaaaccatgg ccatcccgtg gttgcggcaa aaatcgaccg
     8461 aatcctggtc tctgaccgaa ccgccgggct ggatcacggc tgtgatcccg gctttgtgtg
     8521 cgatctccac cgagtcggca aagggaaaaa acgcgtccga ggccagaacg gcgccctgca
     8581 gatcgaagcc gaattccccc gctttggcaa tggcctgttt cagcgcatcg acccgcgagg
     8641 tctgcccgac cccgctgccg atcagttgcc ggtctttggc cagcacgatg gcattggagc
     8701 gggtgtgctt tacgatcttg ttggcgaaga gcagatcgtc ggtctctctc tccgtcggca
     8761 ccctctcggt gaccggccgg agatcatccc gtgtttccgt tttaaggtct ttatcctgct
     8821 caatgatccc gttcagcagc gaacggaact ggcgctcagg aaaatcgtaa cttccctggc
     8881 gcaaaattat tctatttttc ttgctttgaa gcttttccag cgcacttttt ttgtacgaag
     8941 gcgccaggat gacctcgaaa aacagcttgt cgatctcttc ggctgtggct tcatccacct
     9001 ccctgttagt gataagcacg ccgccataag ccgataaggg gtcgccggcc agcgccttcc
     9061 gccaggcgtc ggccaggaca ggccggctgg cgcatccgca ggcattgttg tgcttgagga
     9121 tggcaaacgt cggctcgtcg aactctttga tcagggcaat ggcggcatcg atgtcgccca
     9181 ggttgttgta cgagagctct ttcccgtgca actgggtgaa aacccggccg ggatcgccgt
     9241 ggaaagtccc ctgctggtgc ggattttcgc cgtaacgcaa cttcaccggg ggctggaaat
     9301 ggcccaggat ggccatatcg tacgatgatg tcacaccgaa agccatgccg gcaaagcgcc
     9361 gccgctcctc cagggtcgtg acgccatcct ggtctttcag gatcttaaga aggtcgggat
     9421 actggtttac ggaggagatg atgagaacgt cctcgtaatt ttttgcggcc gcccggatca
     9481 gcgaaatgcc cccgatatcg atcttttcga tgatctcgtc ctcgtcggtc gtccgggcca
     9541 ccgtctcccg gaaggggtac aggtcgacca ccaccaggtc gatgggtgga atgttgtaat
     9601 tcttcgcctg tttcacatct tcctcatttt cacggcgatg cagtataccg ccgaaaactt
     9661 tcggatgaag cgtttttacc ctccctccga agatagaggg gtaagcggtg agcgattcta
     9721 ccgtaatggc cggaatcccc agcccggtaa tgaaatcata ggttccgccg gtggagtaaa
     9781 tcgtaacgcc gttgcggcgc agctcatgca ggatatcctc gaggccggtc ttgtaatata
     9841 ctgaaatcag ggcgcttctg atctttcttt ccataataaa aacgaatatt ttttctttaa
     9901 ggatgaaact tcaaatataa ggcttaatct catctcattt atattatttt taccatctct
     9961 aaggggacaa aaaagatgaa aatcttcctt aaactattga tggaaagcta tttgttcgcc
    10021 gttacggaga tcatccggaa caaggtcaga acattgcttt cgttattggg aatcactatt
    10081 ggtatatttt ccatcatctc ggtattcacg attttcgatt cgatggaatc atccatccgc
    10141 tcaagtatca actctctcgg taacgatgtg ttgtttgtcc agaaatggcc ctgggcaatg
    10201 gggggcgatt atccctggtg gaagtatatc agccggcccg aagcgtcgct taccgatctg
    10261 aaagagatcc agcgccgcag ccaggccagc gagaccagct catatctttc cggattatcg
    10321 gaaacgatca aataccgcga gaaatccatc cagaatgcca atgtgatcat ggtggcggag
    10381 aattacgacc gggtgatgac tgtcgacatc accgaaggac ggtttatctc cccgcttgaa
    10441 ttttccagcg gaaggccggt tgtggttatc ggcagcgata tcgctgaaaa tttcctgtcg
    10501 tcgacaacgg catccgcttt agggaaaaag atcaggattt tcgggatgaa cgtcgaagtg
    10561 atcggcgtga tgaaaaaaca gggaaacaac atgtttggca attcctccga cgaccagttg
    10621 atcgtgccgg tgaatttcgc ccggaatcat gtggatatgc ggtatgccca gggtgcgatc
    10681 attgttaagg ccagaacggg cgtatcgctc gaccagttga gggacgagct gcaggggatc
    10741 atgcgggcgg tgagaaagct caagccgaaa gcggaggata atttcgcaat caaccagacc
    10801 aatgtgatct cgaaaggatt cgaccagtta ttcggcgtga tcggcgtggt cgggtggatc
    10861 atcgggggat tttcgctgct ggtgggtggg ttcggtatcg ccaatatcat gtttgtatcg
    10921 gttaaagaac ggacgcgaat catcggaata caaaaatcaa tgggggcaaa gaactatttc
    10981 attctgctgc aattcctgtt cgaagcggtt tttctgtcgc ttcttggcgg attgctcggc
    11041 cttctgatcg ttttcctgct gacattcgtc atctccaggt cgttcgatat ggagctggtg
    11101 ctcaccgtcg gaaatatcat tcttggtatc accgtttccg ctttgatcgg atttgtttcc
    11161 ggcctggtgc cggcctggaa tgcttcccgg ctcgatccgg tcgaagccat gcgatccaca
    11221 ttctgagagg ctttcagagc ggaggaaaaa tttttctccg gccgaccggg tatttttcac
    11281 ccaaaaagct gattaatcag cagataaatg attaatttta gccagttaaa attatggaaa
    11341 aacgctgggt attaaaaccg cagggaaaca aagatctggt caggcatctg agcaaggttt
    11401 tgaatattaa tgaaaacctg gcgaacctgc tggcgcagag gggtgtggcc agttacgacg
    11461 gggcgaaggc atatttccgc ccgcaactgg aacatttgca cgatcctttc ctgatgaaag
    11521 acatgcataa agccgtggaa aggattgaaa aagccctgaa aaagggagag aagatcctgg
    11581 tttacggcga ttatgatgtg gatggcacca ccgcggtggc gctggtctat acattcctga
    11641 aaaagcttca cgagaaagtc gacttctatg tgcccgaccg gtacagtgaa ggatatggca
    11701 tttcattcaa ggggatcgat tttgccgccg aaaacggctt cagtcttatc attgcgctcg
    11761 actgcggtat caaagccgtg gaaaaagtag cctatgccac cggcaagaaa gtcgatttca
    11821 ttatttgcga ccaccaccgg ccgggagact ccctgcccga agccattgcg atccttgacc
    11881 ccaaacgcga cgactgtact tatccttaca aggagctggc cggatgcggc atcggtttca
    11941 agcttatcca ggcctttgcc tggaagaaca atattgcatt ctcatccttg gaagagtacc
    12001 tcgacctggt ggtggtgagc atcgcctccg acattgtgcc gattaccggc gagaaccggg
    12061 tgctggccta ttacggactg aaacggatca actccaggcc ccgggccggg ctcgaagcca
    12121 tcctcaacgt cagcaacaaa tacagcaaaa ccaacggcga aggccccgac cggcaataca
    12181 tccgcgaact gaccatcaac gacctggtgt tcatcgtcgg accgaggatc aacgccgccg
    12241 gcaggatcaa aagcgcccgc acctcggtcg aactgctcat cacgaaagat ccggcagagg
    12301 ccttcgaact gggcaaccag atcaacaagc agaacagcga ccgcaagacc ctggatacgc
    12361 aggccacgca ccatgccctc gaaatcatcc attccgaccc gaagttcctg aaaaggagga
    12421 gtacggtcct gtaccacccc gaatggcaca aaggggtgat cgggattgtg gcgtcgcgcc
    12481 tgattgaagc gtattaccgg ccgacgatcg tgctgacgca ctccgaggga atgatcaccg
    12541 gctcggcccg ttcgatcaaa ggattcgaca tctacgacgc cgtcgattcc tgcggcgatc
    12601 tcctggagca cttcggaggc catatgtacg ccgccggcct gtcgctcctg cccgaaaatt
    12661 tcgacaaatt caaggaacgc ttcgaagaat atgtgagtac ccacctgacc gaagagctgg
    12721 ctgtaccgga aatagagatc gactcgaagc tgaacctgca ggatgtccag cggaagttct
    12781 acgatattct caggcagttt gccccgttcg gccccggcaa catgtcgcct gtcttccaga
    12841 ccgacaatgt gatcgacgcc ggcaaagcca agatcgtcgg caacaaccac cttaagctgg
    12901 aggtctttca acgcgatgtc cgcagcgaac ctatctcagc catcgctttc ggacagggcg
    12961 aagagaactt cgaacacatc cgcgacggca acccgttcga catctgctac cacatcgaag
    13021 agaacgaatg gaacggccgc aaatccttcc agctcaatat caaagacatc aaatggtcgg
    13081 cggggtaggt tactttatcg ctttcacctt cgtcgacaaa taaatcaaaa acaccccggc
    13141 cacgaccgcc agaaccccga tcacctttga caacgtcccg tcggtgccca tctggctgta
    13201 aaacaacagc aaaccaaaaa ccagcgtgat cacgccgttg atgctgaaca ggtaatggtt
    13261 cgttactttt tttcgcatcc taaccgacag gatgatctgc agcaaaccca ttaacgcggc
    13321 ccagatgccg atcagggtca tgaacaggtc gagcgacttg ccgggattga tcgcgatcag
    13381 cagcccgatc aggaatgcaa aaattgacat gaccatgttc agcacgtagc tcttgttggc
    13441 tttcttattc cggtatgaaa aataaaaaag gaccaacccg cagaccagga taaatatccc
    13501 gaaataccgg acatatttca gcatcatatc ctgcgatacc atcaggacaa atacaccgaa
    13561 caaaatggct atcaacccgt tgatcagaaa aacaaaccag ttggacgttt tcatattatc
    13621 tacaatttac aatttacggt ttacaatcta aaatctagaa cctggaacct ggaacccgga
    13681 acctggaacc tggaaccctt acaccccaat caaaaggtac gtcttcacca ctaaataaat
    13741 cccgaacagc accagcccga ccccggctat cctgttgatc cacacaatat tatgcgtttg
    13801 aaggtatttc ttgactttgt aggcggagaa aattttcagc acgtcgaaaa gcaggaccgt
    13861 gcccagtgcc gacgcgaaaa aggagaccag catcagcatg tcggctttgt aattggctgt
    13921 aaatccgaca acaataccca tccagaatat ccagatgaag gggttggtga aattgataaa
    13981 gaaccctttc aggatgtagg tggtgaagaa cggttcctgc tttttatact ccggatcgct
    14041 ggtgtcgaca ttgatcagca ccggccgccg gtaggtaacg atcccgaaaa tgatgaggac
    14101 aatgccgccc accaggccga gaatggtttt attgctttca aagatttgca cggctcccag
    14161 gaaaccaagc gcgaccagga ccgcatcact gaggaaaacc ccgaatgcca gcaaaaccgc
    14221 ggcccagaat ccccgcagga tgctcgtctg gatctcggcc accagggccg ggccaaatcc
    14281 gaagaaaata gatagggtga gcccgagaag tatgccctct atgatagtct gcatcggttt
    14341 atttgaaatt atactctttc aggtactgtt cccatgctgc cggctggtct ttctttaata
    14401 cccgcgggaa tttgttcgct cctccctctt taccgtgaat cttcatccac gtggagaagg
    14461 ccgccgaagg aagtacttcg acgaaaacgt cttttatagc ggcaatccgt tccacacggt
    14521 agtcgtcgtt gaggattttc agcgcctcgt cgatcttttc ccgggctacc gctgcattca
    14581 gcggatcgtc ggtgcccagg taccatttgt gcgcccacat tgtgtcgtgc ttgatgcccg
    14641 caacggtgaa ctccctgatc tccacgttca tctcgtcgca gagcatttta acggcccggc
    14701 tcatgttttc caccgaaagg tgctcgccgg tgatgctcag gaaatgcttg gtacggcctg
    14761 tgatcacgat ctccgagtgc atgatggagg taaacttgat ggtgtcgccg atcaggtagc
    14821 gccaggcgcc ggcgcaggtc gacaaaagca aggcatactc ttttccctcc tccacctggt
    14881 cgatcgtcaa agctaccggc ttgtcgacca tgttgccttc gtcgtcgaaa ttcaacgagc
    14941 cgcgtcgatc ggtaatggtg ccatcg
//
LOCUS       DAOQXS010000007        14948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4461_length_14948_cov_4.134761, whole genome shotgun sequence.
ACCESSION   DAOQXS010000007 DAOQXS010000000
VERSION     DAOQXS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4461_length_14948_cov_4.134761"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..634)
                     /locus_tag="PKM34_00465"
     CDS             complement(227..634)
                     /locus_tag="PKM34_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82078.1"
                     /translation="MKANDFLCPYCRGHLMANTKVILAARKASGTRGLILFNPQLGEY
                     DVIVHHTFRLAEGEHIDMLCPLCHANLTDQTIHKNLARIKMIDSNGNEYDIYFSEIFG
                     EKCTYKISSDKKIETYGEDADDYQNFWGAEPRY"
     gene            complement(864..1613)
                     /locus_tag="PKM34_00470"
     CDS             complement(864..1613)
                     /locus_tag="PKM34_00470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ82079.1"
                     /translation="MKVLIIFAHPNPLSFTRAILDHFVKGLKEAGHQYEVVDLYKIRF
                     NPVFQDMDYSSFVDKDIPKDIFQQMDIRKLVIELAGGPLKRIIARMYIKNKTDDDLIK
                     LINSQIPKDVQKQQKKVAEADVLVFITQVFWMHFPALVKGWMERVLTYGFAYKLNENG
                     WKGDPDGRIPLLKIKKAIIIHPTFFNEQVYREKGFKVSMEKSIDDWSLKYPGVEKVDH
                     IYFHSILAVSDETRKKYLEIACLKGKNLDLD"
     gene            complement(1622..2194)
                     /locus_tag="PKM34_00475"
     CDS             complement(1622..2194)
                     /locus_tag="PKM34_00475"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNQ82080.1"
                     /translation="MLNFLVLLIVFLIFSLVFFVVIRGILNKIPIIGKPPVKTVFFIT
                     AKMMVVVNLTFLVMAGLNIKLNALFDPVFALRLIALVLLVTGTAILYVTTIQLNKDLI
                     FGLPDSEKGRLQTKGLFAFSRHPFYLGFILILFSSCLLYPNPLNITAFLTAWIIHHFI
                     MIEEEKFLETVYGEEYRIYRQKVGRYLTIK"
     gene            complement(2281..3063)
                     /locus_tag="PKM34_00480"
     CDS             complement(2281..3063)
                     /locus_tag="PKM34_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ82081.1"
                     /translation="MFKVFKYSFFDLVRSRWIYAYLLFFLLVTTGLVWSGSDLSRALI
                     SLMNILLILCPLIGTLFGALYYYNSREFIDLLLALPLPRRSIFLGKYLGLAISLSASF
                     LLGIGLPMVVFGVFSSGLLTNFLTLLATGIFLTFIFTAIAFVVALKSENPIRGFGMAI
                     FIWLFLSVIYDGILLVVMMYFESYPLDRFALIASLFNPVDLSRILIMLQLDISALMGY
                     TGAVFRKFLGTGTGIAVAYSALACWIVVPVLLFIRCAGRKDF"
     gene            complement(3056..3763)
                     /locus_tag="PKM34_00485"
     CDS             complement(3056..3763)
                     /locus_tag="PKM34_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82082.1"
                     /translation="MKIIDLHKAYGRFGVLKGVDLELKDRAITAVLGPNGSGKTTLIK
                     CLLGMVIGQKGDILYHGEPVGRGWAYRSDIGYLPQIARFPENLKVKELVAMIRDIRNQ
                     PAETDALVALFGLDAFMNKPLRQLSGGTRQKVNILIAFMFDSPVYILDEPTVGLDPVA
                     LIRFKELLLAEKRKGKAILMTTHIVHLVEELADEVVFLLEGKVYFKGDVKALKELNKS
                     ESLEEAIAKILELEKHV"
     gene            complement(3760..4953)
                     /locus_tag="PKM34_00490"
     CDS             complement(3760..4953)
                     /locus_tag="PKM34_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNQ82083.1"
                     /translation="MPVFSDTIYVSEDHVPTISEALSMSEPGDVIIVQPGLYREGNII
                     INKKITLTGIKYPVADGSDSNEVFTIMSDSVVISGFQVQNCGVSYVKDRAGIRIDQQK
                     GCVIENNRLINTFFGIYLKNASDCLILNNYIEGVAQDEFSSGNAIHLWYSKNITVEGN
                     TCLQHRDGIYLEFVENSMIRKNHSERNLRYGLHFMFSNNDSYVENTFRDNGAGVAVMF
                     SHHITMQNNHFLKNWGSSAYGLLLKDISDGEMTGNVFRENTVGIYGDGANRIRMEGNE
                     FISNGYALKILGSCSGNTITGNNFISNTFDVFTNTSGNTNTFESNFWSDYTGYDLDRD
                     NKGDVPYRPVKLFSYITGRVPASMILLRSPFVDLVDFAEKVMPSITPPTLEDASPLMK
                     MIILN"
     gene            complement(5135..5590)
                     /locus_tag="PKM34_00495"
     CDS             complement(5135..5590)
                     /locus_tag="PKM34_00495"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNQ82084.1"
                     /translation="MKKLFYIIPLMLFTISCTIKPEPIHYGEDNCILCQMTIMDHRYG
                     TEMVTSKGKIFKFDSIECMVDYFKETSKKGEEYRYILFTPFDQPGKLTDALTGYVLHS
                     KNLPSPMGMYLTAFEDKAAAMRFQQEKGGRIYDWKGLVEFFEVMKREMR"
     gene            complement(5592..6164)
                     /locus_tag="PKM34_00500"
     CDS             complement(5592..6164)
                     /locus_tag="PKM34_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82085.1"
                     /translation="MKNLPKYLMILAALLLVFLFFFPIWKITLFAPQYPGGISMYIWI
                     NQITGDTPGTLQNINILNHYVGMQMIEPDSIPELKYFQFIIAGMGVMALLFAWLNKRP
                     LILAWVILFIVLGAAGIYDFYLWEYDYGHNLSPTAPIKVPGMAYQPPLFGKKELLNFI
                     AYSYPHWGSLFFGLSILSGTLAFWFHKKKN"
     gene            complement(6270..8228)
                     /gene="nosZ"
                     /locus_tag="PKM34_00505"
     CDS             complement(6270..8228)
                     /gene="nosZ"
                     /locus_tag="PKM34_00505"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNQ82086.1"
                     /translation="MKQKLTLFSILFGLGMLFACTPKQPGTTGAGNAADAASRVYVAP
                     GQYDEFYAFLSGGFSGQLSVYGLPSGRLLKVIPVFSVDAEKGYGFNEETKPLLNSSFG
                     FLPWDDAHHPELSQTNGVPDGKYIFINGNNTPRIAKIDLSTFETTEIIEIPNSGGNHS
                     SPFTTENTEYVVAGTRFSVPFPQKDVPIDSYKEHFKGALSFISVSPEGHMDLAFQVLL
                     PAFNYDLAHSGKGASHGWTFFTSYNAEQSNTLLEANASQFDKDFIAAVNWKKAEEYVK
                     AGKAKEMPASYFHNVYDENSHMATSTEKKTVKVLIPSECPDMVYFLPTPKSPHGVDVD
                     PTGEYIVGAGKLSADMAVHSFSRMLKAIENKAFETMIDGIPVLKYEEVLGGMVSSGGL
                     GPLHTEFDGKGNAYTTFFISSEVVKWKVGSWEVLDRVPCYYSVGHLMIPGGDSKQPFG
                     KYMLALNKITKDRYLPTGPELNQSAQLYDISGEKMQLLLDFPTVGEPHYAQAIPADLV
                     KPRSKQIYKLGENTHPHAARSEADARVVREGNVVRIYMTAIRSHFAPDNIEGIQVGDK
                     VYFHVTNLEQDWDVPHGFAVMGARNAELLIMPGQTETLFWEPDRPGVFPMYCTDFCSA
                     LHQEMQGYVRVSAKGANIPLKWWVGSQQ"
     gene            complement(8312..8776)
                     /locus_tag="PKM34_00510"
     CDS             complement(8312..8776)
                     /locus_tag="PKM34_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNQ82087.1"
                     /translation="MKTIMTSFACLAIVTIMVSCGGTSEQKPAAPGTVPAAKPASDDP
                     KANKGIGPVKELTLGELDPAMAAEGEEIFKKMCSACHKPAEKFIGPAPKGILDRRSPE
                     WIMNMILNPEEMVKYDEIAKKLLIEANLAPMANQHLTQEQARAVLEYFRTIK"
     gene            complement(8898..9392)
                     /locus_tag="PKM34_00515"
     CDS             complement(8898..9392)
                     /locus_tag="PKM34_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNQ82088.1"
                     /translation="MSNAHSRPSWDEYFLDLAKAAARRATCDRGRSGCVIVRDKQVLV
                     TGYVGSPMGLPHCDDVGHLMKKVVHDDGTVTQHCVRTVHAEQNAICQAARRGIALDGG
                     TLYCRMTPCRTCAMLIINCGIKRVVCQNRYHAGNESEAMFLEAGVKLDYFSDEVVEYE
                     NQKG"
     gene            complement(9404..9976)
                     /locus_tag="PKM34_00520"
     CDS             complement(9404..9976)
                     /locus_tag="PKM34_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ82089.1"
                     /translation="MIIIGITGTLGAGKGTIVDYLVNQRGFLHFSVRAFISEEIVRRG
                     MKVNRDSMVVVANDLRRNNSPSYITDCLYEQAMEANRNSVIESIRTPGEVYSLRKKGR
                     FLLISVDADPKIRYDRISRRKSETDHIDYETFLQNERREMTSDDPNAQNLAKCIEMAD
                     LKFFNNGTIEQLNRQVEAAMGNLEIGQFGN"
     gene            10068..11711
                     /locus_tag="PKM34_00525"
     CDS             10068..11711
                     /locus_tag="PKM34_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ82090.1"
                     /translation="MLKENFSRYLADSITRNWDYRALTDYQGESFYYHDVAGHIVRLH
                     QFFREAGIRPGDKIGLIGKNSARWGILYITTVTYGAVIVPILPDFKPEDLQQIVDHSD
                     AVILFAEEGIFEKMDRANMPKLKCVVSVTSLEFLAGDEEIRAVHSRNEFHKIVPGFYK
                     DEDKPAFPEIPGETLAVISYTSGTTGFTKGVMLTHNNLAANMRYAQQNMPLNPGDRIV
                     SFLPLAHTFGAAFEFLFPFTLGCDITILTKTPSPQVIIKAFQEVKPSLILSVPLVIEK
                     IYKKQVLPAIRKPHMKLLLMLPGLKNIILKKIREKLVAVFGGNFRELVIGGAAFNAEA
                     EEFFRQMKFPFTVGYGMTECGPLISYVGWKDSRPGSAGKAVDTLEVKIDSPDPANKSG
                     EIIVRGDNVMLGYYKNDEATQEVLGADGWLYTGDLGRMDKDGHIYIRGRIKSVLIGSS
                     GKNIYPEEIEAQFNNKPFIAESLVVQRGEKLIVLISPDFEEMEKAGQKEENLAEIYNH
                     YLKTVNHHLPGFMQVSKYEIHPKEFQKTPKRSIKRFLYS"
     gene            complement(11689..12711)
                     /gene="amrS"
                     /locus_tag="PKM34_00530"
     CDS             complement(11689..12711)
                     /gene="amrS"
                     /locus_tag="PKM34_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ82091.1"
                     /translation="MSVHPASYSVKLQENKVKCTLCPHLCVIADGKTGICRVRKNDGG
                     TLISEVYGKACCLRFDPIEKKPLYHFYPGSSILSVGSVGCNLRCKFCQNWEISQTCAE
                     DYPYLKPASVDEIIRMAGERHDNAGIAFTYNEPVIWFEFMVDIARKSKEKGLRNVMVS
                     NGYINAAPLSELLGCIDAFNIDLKAFTDDFYRRVTGGRLQPVRDTLSMIRKHNRHLEL
                     THLVVTGLNDDDDQFEEMVRWIGGELGPETPLHLSRYFPNFELENKATPLPKLLHLYE
                     MATQYLRYVYLGNVAADEGRITVCPDCGASLIERIGYSTYKKGLDAKGRCRKCSRPVI
                     NYIETA"
     gene            complement(12754..13344)
                     /locus_tag="PKM34_00535"
     CDS             complement(12754..13344)
                     /locus_tag="PKM34_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNQ82092.1"
                     /translation="MDSICVFCGSSMGKKPEYKKAAEELGRLLLERNIRLVYGGANVG
                     LMQILADTVLKGGGEVIGIMPQMLIEKEVAHANLTKMITVGSMSERKSRMVELSDAFI
                     AMPGGFGTLDELAEVITYNQLRLTDKPIGILNTAGYFDPLVAFFDHITEEGFLRHEHR
                     QNLIVDDNVGVLLRKMSEFQPVAMDKWIEDIKKESL"
     gene            13535..14512
                     /locus_tag="PKM34_00540"
     CDS             13535..14512
                     /locus_tag="PKM34_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HNQ82093.1"
                     /translation="MNELYPLKFKPVFDDRIWGGEKIRTHLGLDFAPLNRCAEAWILS
                     GYEGKQTVVSNGFLEGNEINELIEIYMDDLVGEKVYENTGETFPLLIKFIDSRDWLSV
                     QVHPDDELAQKRGMPNGKTEMWYVIDAEENAQLIAGFNRKITQKQYLEHLHNKTLPEI
                     LNYEKVRKDDAIFMPAGRIHSLGPGVLLAEIQQTSDATYRLFDWHRKDSQGKERELHT
                     ELALAAIDFDAFSDNKTNYKPRLNETVDLVSCPYFTTNLIELAQPLRKNYEEIDSFVV
                     YVCLEGAMRIKCGDSPTTVAKGEVVLIPAMFDVVEIYPDGNVKFLEVYI"
     gene            14525..>14948
                     /locus_tag="PKM34_00545"
     CDS             14525..>14948
                     /locus_tag="PKM34_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNQ82094.1"
                     /translation="MKKVIVFFILVFSGINLLAQEGILEKTIPAIDIKTLQGETVNTS
                     SIDNQGKPMIISFFALWCKPCMRELTAISDVYADWQEEIGVKLVAISIDDSRSVANVL
                     PTVNGNGWEYEFYSDVNGDFKRAMGVNMIPHVFVVDGNK"
BASE COUNT         3859 a         3727 c         3580 g         3782 t
ORIGIN      
        1 tcgccgtgga taaagacggc aattacacga tgacattcaa caccagcggg atgtttcgtg
       61 gggtggtacg atccgacgga aagcgcatgg tcgctatctt tgcggaacaa taatttttca
      121 gtcgtcagtc gtcagtcgtc agtcgtcagt cgtcagtcgt aattaatgga cgctatcatt
      181 gtcatttctg accaacgact aatgactaat gactaatgac caacgactaa tatctcggtt
      241 ccgcccccca aaaattctgg tagtcgtcag cgtcttcccc gtacgtttca attttcttat
      301 cgctgctgat cttgtaagtg catttctcgc cgaagatctc cgaaaaataa atatcgtatt
      361 cgttcccgtt gctgtcgatc atcttgatgc gggccaggtt tttatggatc gtctggtcgg
      421 tcaggttggc atggcagagg gggcaaagca tatcgatgtg ttctccctca gccagcctga
      481 aggtatggtg gacgatcaca tcatattcgc ccaactgtgg gttgaagagg atcaggccgc
      541 gtgtgcccga cgctttgcgg gcagccagga tcactttggt attggccatc aggtggccac
      601 ggcaataggg acaaagaaaa tcgtttgctt tcatggcgat tggaaataaa gggcaaagat
      661 agttcaaaga ttggttcgac tgttcaattg ttcgattgtt acagggttac gggttaaggg
      721 ggtccctcac cgcctccggc ggttcgggat gacgtttgag atgaaagaaa aagtcgcgcc
      781 agcgacgcca gtatggtagc ccccgattaa atcgggggta acgtacggca aattccattc
      841 ggagtgctgt aggcacggca gaattaatcc agatcgagat ttttgccttt taggcaagca
      901 atttccaggt atttcttcct agtctcatcg cttaccgcaa ggatcgagtg gaaatagata
      961 tggtcgacct tttcaactcc aggatatttc aggctccaat catcgattga tttttccatg
     1021 gaaactttaa atcctttctc cctgtatacc tgttcattaa aaaaagtagg atggatgatg
     1081 atggcttttt tgattttcag aagaggtatc cttccgtcag ggtcaccttt ccacccattc
     1141 tcatttaatt tataagcaaa tccatacgtc aggacccttt ccatccaacc ttttaccaac
     1201 gccgggaagt gcatccagaa tacttgcgtg ataaagacca gaacatcggc ttctgcaact
     1261 ttcttttgct gtttctggac atctttcggt atctgcgagt tgatcagctt tatcaggtca
     1321 tcatcggtct tattcttaat atacatccgg gcaattatcc tttttaacgg cccgccggca
     1381 agttcaatga ccaacttcct gatatccatc tgctgaaaga tgtctttagg tatatcttta
     1441 tcaacaaaag aggaataatc catatcctgg aaaacaggat tgaatctgat cttgtacaaa
     1501 tcgaccacct catactggtg accggcttcc ttcaggcctt tgacgaaatg gtcgagtatt
     1561 gccctggtaa acgacaatgg atttggatga gcaaaaatga tcagtacttt catggttcat
     1621 ttcatttaat tgtcaggtat cttccaactt tttgcctgta tatcctgtat tcctccccat
     1681 aaactgtttc aagaaacttt tcctcttcta tcataataaa atgatggatg atccaggccg
     1741 tgagaaaagc ggtaatattc aacggattgg gataaagaag gcaggaggag aagagtatca
     1801 ggataaagcc aagataaaaa ggatgacggc tgaaagcaaa aaggcctttg gtttgcaggc
     1861 gacccttttc agaatcaggc aaaccgaaga tcaggtcttt gttgagttga atggtcgtca
     1921 catatagaat agcagtgccc gtgaccagca gaacaagggc gatcagcctg agggcaaata
     1981 caggatcaaa taatgcattg agctttatat tcaatccggc cattaccaag aatgtgaggt
     2041 tgaccactac catcattttg gcggtaatga agaagactgt tttaacggga ggtttgccta
     2101 tgatgggtat tttattcagg ataccgcgga taacaacgaa aaagacaagt gaaaaaatca
     2161 aaaaaacgat gagaagaacc aggaaattta acatgggagg cgaatttacc cccaaatata
     2221 acatttatat tgaataaata tcgattcttt ttattaaaag gggacgagat cgcgaacgga
     2281 ctaaaagtct ttcctccctg cacaccggat aaacagtaat accggtacca ctatccagca
     2341 tgccagtgcc gaataagcca cggcaatccc tgttccggtc cccaaaaatt tgcggaaaac
     2401 ggcgccggtg tatcccatca gcgccgaaat atctagctgc agcatgatca ggatgcgcga
     2461 caggtcgacg ggattgaaca gggaggcgat caatgcaaac cggtcgagcg ggtaactctc
     2521 gaaatacatc atgacaacca gcaggatacc gtcgtagatg accgagagga acagccagat
     2581 aaaaatcgcc attccgaagc cgcggattgg attttccgac ttcagcgcca ccacgaaggc
     2641 gatggcggta aaaataaacg tgaggaaaat tcccgttgcc aggagggtca ggaaattcgt
     2701 caacagcccg gatgaaaaca cgccgaaaac gaccatgggc aggccgatcc ccagtaaaaa
     2761 gctggctgaa agcgaaatgg ccagccccag gtatttgccc aggaaaatcg aacggcgcgg
     2821 cagcggcaaa gccagcagga gatcgataaa ttcccttgag ttgtagtagt atagcgctcc
     2881 gaaaagggtt ccgatcagcg ggcagaggat cagcaggatg ttcatcaggc tgatcaaggc
     2941 cctggacaga tcgcttcccg accagaccag cccggtggtc accagcagga aaaacagcaa
     3001 atatgcgtaa atccagcggc tccgtaccag gtcgaaaaag ctgtatttga ataccttaaa
     3061 catgcttttc cagttccagt atttttgcga tggcctcttc caggctctcc gatttgttca
     3121 gctctttaag agccttcaca tcgcctttga aatagacctt cccttcgagc aggaacacta
     3181 cctcgtcggc cagctcctcc accaggtgta caatatgcgt ggtcatcagg atcgctttgc
     3241 ctttccgttt ctctgccagc agcaactcct tgaaacggat cagcgccacg ggatccaagc
     3301 caaccgtagg ctcgtcaagg atataaacgg gagaatcgaa catgaaggcg atgaggatgt
     3361 tgaccttctg ccgcgtgccg cccgacaatt gacgcaaagg cttgttcata aatgcgtcca
     3421 ggccgaacag ggccaccagg gcatcggttt cggcaggctg gtttcggata tcgcggatca
     3481 tagccacgag ttccttcact ttaaggttct ccggaaaacg ggcaatctgc ggcaaatatc
     3541 ctatatccga gcgataagcc cagccgcggc caacaggttc gccatgatac agaatatccc
     3601 ccttctgccc tatgaccatc cccagtaaac attttatcag cgtggttttc cccgatccgt
     3661 tagggcccag cactgccgtt attgctctgt ccttcagctc caggtctacc cctttcagca
     3721 cgccgaacct gccgtaggcc ttatgtaaat ctatgatctt caattcagta tgatcatttt
     3781 catgagaggc gaagcatctt ccagcgttgg aggtgtaatc gatggcatta ccttttcggc
     3841 aaagtctacc aggtcgacaa acgggctgcg cagcaggatc atcgaagcgg gaacccggcc
     3901 ggtgatatag gagaaaagct tcaccggtct gtagggcaca tcgcctttgt tgtcgcggtc
     3961 gaggtcatat ccggtgtaat cgctccagaa attggattcg aatgtattgg tattgccgga
     4021 ggtattggta aaaacatcaa aggtattgga aatgaaattg ttcccggtga tcgtattgcc
     4081 ggaacaactg cccaggatct tgagggcata gccgttggaa ataaactcat tgccctccat
     4141 tcttatccgg ttggcaccgt cgccgtaaat cccgacggta ttctcccgga atacattccc
     4201 tgtcatctcc ccgtcggaaa tatcttttaa taataaccca taagccgaac tgccccagtt
     4261 ctttagaaaa tgattatttt gcatggtaat gtggtgcgag aacatgaccg caacgcctgc
     4321 gccgttatcc cggaaagtat tctccacata actgtcgttg ttggaaaaca taaaatgaag
     4381 accgtaccgg aggtttcgct cactgtgatt cttcctgatc atgctgttct ccacgaattc
     4441 aaggtaaatt ccgtcacggt gctgaaggca ggtattccct tccacggtaa tgttttttga
     4501 ataccataaa tgaatggcat tgcccgatga aaattcatcc tgtgcaacgc cttcaatata
     4561 attgttcagg atcaggcaat cactggcatt tttgagataa atgccaaaaa atgtattgat
     4621 caggcgattg ttttcgatga cacatccctt ttgctgatcg attctgatcc cggccctgtc
     4681 tttcacatag ctcaccccgc agttctgcac ctggaatccg ctaattacaa cgctgtcgga
     4741 cataatggtg aacacttcat tcgaatccga gccatcggcc acagggtact ttatccccgt
     4801 aagggtaatt tttttattta tgatgatatt cccttcccgg tataaccccg gctgaacgat
     4861 gatgacatca cctggttcag acatagataa tgcttctgag atcgtgggaa catgatcttc
     4921 agaaacataa atggtatccg aaaaaaccgg cataccaatg ataatcaaga gataagcaaa
     4981 agttattcta cttggcctca cacaaagtcc cacaaaggtt ttacaaagaa tcaaaatatc
     5041 aataaatcaa aagttcaaaa gttcaaaagt tcaaaagttc aaaagctcac aagctcatac
     5101 gttcacaaaa tcacaatatc acaaaatcac aaaatcacct catctctctc ttcatcactt
     5161 caaaaaactc cacaagccct ttccagtcat atattctccc gcctttttcc tgttgaaacc
     5221 gcatagccgc cgctttatcc tcaaaagccg taaggtacat tcccatcggg ctgggcaggt
     5281 ttttgctgtg cagcacataa ccggtgaggg catcggttaa tttacccggc tgatcaaaag
     5341 gagtaaaaag gatataacgg tattcttcac cttttttcga cgtttcctta aagtaatcca
     5401 ccatacattc tatggaatcg aatttgaaga tttttccttt tgacgtaacc atctcagtcc
     5461 cgtacctgtg gtccataatg gtcatctggc aaaggatgca attgtcttca ccgtaatgaa
     5521 taggctccgg tttaatggtg caagagatgg taaagagcat caaagggatt atataaaaca
     5581 gcttcttcat tttaattctt ctttttatga aaccagaaag caagggtacc ggatagaatg
     5641 gataatccga agaacaggct gccccagtgc gggtaggaat aggcaataaa gttaagcagt
     5701 tctttcttcc cgaacagcgg gggctggtag gccatccccg gcaccttgat cggggcggtg
     5761 ggagaaaggt tatgaccata gtcgtattcc cagaggtaga agtcgtagat tccggcagca
     5821 cccaatacaa tgaaaaggat aacccacgcc aggatcagcg ggcgtttgtt cagccaggca
     5881 aacaaaaggg ccatgactcc cattcctgca atgatgaact ggaagtactt cagttcgggg
     5941 atggaatcgg gttcgatcat ctgcattccg acgtaatgat tcaggatatt gatgttttgc
     6001 agtgtcccgg gggtatcgcc ggttatctgg ttgatccaga tgtacatcga gataccgccg
     6061 gggtactgcg gagcgaacag cgtgatcttc cagatcggga aaaagaagag gaagaccagc
     6121 agaagcgctg caaggatcat caggtatttc ggaagatttt tcatgtgttg atattgattt
     6181 ttgctccgac gactgaagcc gtcggttcca ttattgtttt actttggaac caacggcttt
     6241 agtcgttgga ctacctcaca tttggttgat tattgctgag agccgaccca ccatttcaac
     6301 ggaatgttgg ctcctttagc ggatacccgc acataaccct gcatctcctg gtgcagcgcc
     6361 gagcaaaagt cggtgcagta catcggaaaa acgcccgggc ggtcgggctc ccagaagagc
     6421 gtttcggtct gtcccggcat gatcagcaac tctgcattac gggcgcccat gacggcaaag
     6481 ccatgaggca cgtcccagtc ctgctccagg ttggtcacgt ggaaatagac cttgtcgccc
     6541 acctggatgc cttcgatgtt atccggagcg aaatggctgc ggatggcggt catatatatg
     6601 cgaaccacat tgccttcacg caccaccctg gcatccgctt cgctacgggc agcgtgcgga
     6661 tgggtatttt ctcctagctt atagatctgc tttgagcgtg gctttaccag gtcggcaggg
     6721 atagcctggg cgtagtgagg ttctccaacg gtcgggaaat cgagcaaaag ctgcatcttt
     6781 tcgcctgaga tatcgtaaag ctgcgccgac tggttcagct ccggaccggt aggcaggtag
     6841 cggtctttcg tgatcttgtt cagggccagc atgtatttcc cgaaaggctg cttcgagtcg
     6901 ccgccgggga tcatcaggtg accgaccgaa taatagcagg gtacccggtc gagcacctcc
     6961 catgagccga ccttccactt caccacttcc gatgaaatga agaatgtcgt ataggcattt
     7021 cctttgccgt cgaactccgt gtgcagcggt ccgaggccgc cgctggagac catcccgcca
     7081 aggacttctt catactttag caccggaatc ccatcgatca tcgtctcaaa tgccttgttt
     7141 tctattgctt tcagcattct ggaaaacgaa tggactgcca tgtcggccga taatttgcct
     7201 gcgccaacaa tgtattcgcc tgtcggatcg acatcaaccc cgtgaggtga tttcggggtc
     7261 gggaggaaat agaccatatc ggggcattcc gacgggatca gcaccttcac cgttttcttc
     7321 tccgtagagg tggccatgtg gctgttttca tcatacacat tatggaaata gctggccggc
     7381 atctcttttg ctttgccggc ttttacgtat tcctccgctt ttttccagtt taccgcggcg
     7441 atgaaatcct tgtcgaattg cgacgcgttg gcttcgagca atgtattcga ctgttcggca
     7501 ttatagcttg tgaagaaagt ccagccatgc gaagcgccct tgccggaatg cgcgagatcg
     7561 tagttaaatg ccggcagcag cacctggaaa gccaaatcca tatgtccttc cgggctcacg
     7621 gaaatgaacg atagcgcacc tttgaaatgc tccttgtagg aatcgatagg cacgtctttt
     7681 tgcgggaaag gcacactgaa gcgggtgccc gcaactacat attccgtgtt ttcagtggta
     7741 aatggagaac tatggttccc cccgctgttc gggatctcga tgatctccgt cgtttcgaaa
     7801 gtggacagat cgatttttgc gatcctgggt gtgttattcc cattgatgaa gatgtactta
     7861 ccatcgggaa cgccattggt ctgcgacagt tccgggtggt gtgcgtcgtc ccatggcagg
     7921 aatccgaacg aggaattgag aagcggcttg gtctcttcgt tgaaaccata gcctttttcg
     7981 gcatcgaccg aaaaaaccgg gatcactttc agcagtcttc ccgaagggag gccataaacg
     8041 ctaagctggc ctgaaaaccc accggaaagg aatgcataga attcgtcgta ttgcccgggt
     8101 gcaacataca ccctggaggc tgcatcggca gcattaccgg ctcctgtcgt tccgggttgc
     8161 ttcggcgtac aggcaaaaag catacccagg ccgaagagga tcgaaaatag tgttagtttc
     8221 tgtttcatag gttattgggt tgagagattt gtcataaggt ggtcataagg tggtcataaa
     8281 gtggtcgtga tacttacagc cgactgccga cttattttat cgtccggaaa tactccagca
     8341 cagcacgtgc ttgttcctgt gtaagatgct ggttggccat cggggcgagg tttgcttcga
     8401 tgagcagctt tttggcaatt tcatcatact tgaccatctc ttccgggttc aggatcatgt
     8461 tcatgatcca ctccggagag cggcggtcga gaattccctt tggcgccggg ccgatgaatt
     8521 tctcagccgg tttatggcag gcagagcaca tttttttgaa tatctcctcc ccttccgccg
     8581 ccattgccgg atcgagttcg cccagtgtca gttctttgac cggtccgatc cctttgttgg
     8641 ctttcgggtc gtcagaagcc ggtttggctg ccggcaccgt accgggtgcc gccggtttct
     8701 gttccgaagt accgccacac gacaccatta ttgtaacaat tgccagacag gcaaaagaag
     8761 tcatgattgt tttcattatt tcttattaat ttgattgata atttattgcg gtaaagatat
     8821 atgtcacaat aggaacaata tgtaacatat gttacaagaa aaggaaattg atcacaaaaa
     8881 ttcagaaaat caaaatatca gcccttctga ttctcatatt ccaccacctc atcgctgaaa
     8941 taatcaagct tgactccggc ttcaaggaac atcgcttccg attcgttgcc ggcatgatac
     9001 cggttctggc aaaccacccg cttgatcccg cagttgatga tcagcatggc gcaggttcgg
     9061 cagggagtca tccggcagta gagcgttccg ccgtcgaggg cgatgccgcg ccgggccgcc
     9121 tggcaaatgg cattctgctc ggcatggacg gtacggacgc aatgctgggt gaccgtgccg
     9181 tcgtcatgca ccaccttctt catcaggtgt ccgacgtcgt cgcaatgtgg caaacccatc
     9241 ggcgagccga catatccggt caccagcacc tgtttatcac ggacaatgac gcagccgctg
     9301 cgcccacggt cgcatgtcgc ccttctagcc gccgccttgg ccagatccag gaaatattcg
     9361 tcccacgaag gacgggaatg tgcattactc atttccaaat tgtctaattt ccaaattgcc
     9421 caatttccaa attgcccatt gcagcttcca cttgccggtt caattgctca attgttccat
     9481 tgttaaaaaa ttttaaatcc gccatttcga tacatttcgc aaggttctgc gcattcggat
     9541 cgtccgaagt catttctctt cgttcgtttt gcagaaaggt ttcgtaatca atatgatccg
     9601 tttccgattt tcgccggctg atccggtcat acctgatctt cgggtcggcg tcaacagaaa
     9661 tcaggaggaa ccggcctttc tttcttaacg aataaacctc acccggcgtc cggatacttt
     9721 cgatcacact gttcctgttt gcttccatcg cttgttcata gaggcaatcg gtgatataag
     9781 acggcgaatt gttccttcgc aggtcgttgg caaccaccac catcgaatca cggttcacct
     9841 tcattcctct tctgacaatt tcttccgaaa taaaagccct gacagaaaaa tgaaggaatc
     9901 cccgttgatt gaccaggtaa tcgacgatcg tcccttttcc tgctcccagt gttccggtta
     9961 ttccaatgat gatcatacta ttgatttcaa aacaaactta taaaataaac aggtggtttc
    10021 aacctttcaa ataatatcta actttgattt cttttttaaa ccaaattatg ctgaaggaaa
    10081 acttttcacg ttacctggca gacagcatta ccaggaactg ggactaccgc gcacttacgg
    10141 attatcaggg agagagcttt tactaccatg atgttgcagg acatatcgtc aggcttcatc
    10201 aattcttcag ggaagcgggc atacgacccg gcgacaagat tggcctgatc ggtaaaaact
    10261 cggcccgctg gggtatcctg tacatcacca ccgttaccta cggcgcggtg atcgtgccaa
    10321 ttctgcccga tttcaagccc gaagatctgc aacagatcgt cgaccattcc gatgctgtta
    10381 tcctgtttgc cgaagagggt attttcgaga aaatggacag ggcgaatatg ccaaagctca
    10441 aatgtgtggt ctcggtcacc tcgctggaat tccttgcggg cgatgaagag atcagggctg
    10501 ttcactcgcg taacgaattc cataagatcg tcccgggttt ttataaagat gaagataaac
    10561 cggcatttcc ggagatcccc ggtgagacgc tggctgtgat cagttatacg tcgggcacga
    10621 ccggatttac caagggggtc atgctgacac acaacaacct ggcagcgaat atgcgttatg
    10681 cccagcagaa tatgccgctg aacccgggcg accggatcgt gtcgttcctg ccattggccc
    10741 acaccttcgg cgccgcgttc gagttccttt tcccattcac actgggctgc gatatcacca
    10801 tcctgaccaa aaccccgtca ccgcaggtca ttatcaaggc gttccaggag gtgaagccct
    10861 cgctgatcct ctccgtgccg ctggtcatcg agaaaatcta caaaaaacag gtcctccctg
    10921 ccatccgcaa accacatatg aagttgctgc tgatgttgcc cgggttgaaa aatattatcc
    10981 tgaagaagat ccgggaaaag ctcgttgccg ttttcggcgg aaatttccgc gaactggtga
    11041 tcgggggtgc ggcctttaat gccgaggcag aggaattttt tcgccagatg aagttcccat
    11101 tcaccgttgg ctacggcatg accgaatgcg ggcctctgat cagctatgtc ggatggaaag
    11161 attccaggcc cggatcggca ggtaaggcgg tggataccct tgaagtgaag atcgattcgc
    11221 ccgatccggc caataaatcg ggcgagatca tcgtcagggg cgataatgtc atgctggggt
    11281 attacaaaaa cgatgaagcg acacaggaag tactcggtgc agacggctgg ttgtatacag
    11341 gcgacctggg gagaatggac aaggatggcc atatctatat ccgtggccgg ataaaaagcg
    11401 tcctgatcgg ctccagcgga aagaatatat accccgaaga gatcgaggcc cagttcaata
    11461 acaagccgtt cattgccgaa tcactcgtcg ttcagcgtgg tgaaaagcta attgtgctga
    11521 tcagccccga ttttgaagag atggaaaaag cggggcagaa agaggaaaac ctggctgaga
    11581 tttacaacca ctacctgaaa accgtcaacc accacctgcc gggatttatg caggtttcaa
    11641 aatacgagat ccatccgaag gaattccaga aaacgccaaa acgtagtatc aagcggtttc
    11701 tatatagtta atcaccggcc tgctgcactt ccggcatctc cctttggcat cgagcccttt
    11761 tttataggtt gaatagccga tccgttcgat caggctggcg ccacaatcgg ggcaaacggt
    11821 aatccgccct tcatcagcag ctacattgcc caggtaaaca taccggaggt attgagtcgc
    11881 catttcatac agatgcagca gcttgggcaa cggtgtcgcc ttgttttcca gttcaaagtt
    11941 cgggaaatag cgcgacaggt gcagcggtgt ttcaggaccc agctcaccgc cgatccacct
    12001 caccatctct tcgaattggt cgtcatcgtc gttcagacct gtcaccacca ggtgtgtcaa
    12061 ctcaaggtgc cggttgtgct tcctgatcat cgacaaagtg tctctgaccg gctggagcct
    12121 tcctcccgta accctgcggt aaaaatcatc ggtaaaggct ttaaggtcga tattgaaagc
    12181 gtcgatgcag ccaagcagct cagaaagcgg cgcagcattg atatacccgt tcgacaccat
    12241 cacgttccgc aatcccttct ccttcgattt ccgggctatg tcgaccatga actcaaacca
    12301 gataacaggt tcattgtaag tgaatgctat accggcgttg tcgtgccgtt cgcccgccat
    12361 ccggatgatc tcgtcaaccg aagccggctt gagataagga taatcttcag cgcaggtctg
    12421 ggagatctcc cagttctggc aaaacttgca gcgcaggttg caccccacgg atccgaccga
    12481 taaaatcgaa ctgcccgggt aaaaatgata caacggcttt ttctctatcg ggtcaaaacg
    12541 caggcagcag gctttaccgt aaacttccga aataagcgtc cctccatcgt tcttcctgac
    12601 tctgcaaata cctgtcttcc catccgctat aacgcacagg tggggacaca aggtgcactt
    12661 aaccttattt tcctgaagct tgaccgagta tgaagcagga tgcacggaca ttttgtaaaa
    12721 ataatcattt ttcgtacctg taagcctaga agctcataag ctttcctttt tgatatcctc
    12781 gatccattta tccatcgcaa ccggctggaa ttcagacatc tttctcaaca acacgccgac
    12841 attgtcatct acgatcaggt tttgacggtg ttcgtgcctg agaaagccct cttccgtgat
    12901 atggtcgaag aaagccacca gaggatcaaa ataaccggca gtattgagga tgccgatcgg
    12961 cttgtcggtg agccggagct ggttgtaagt gatcacctcg gccagttcat ccagtgtgcc
    13021 gaatccgccg ggcatggcaa taaaggcatc ggatagttct accatcctgc tcttgcgttc
    13081 actcatgctt cctacggtga tcattttcgt gagatttgca tgtgcgacct ctttttcgat
    13141 cagcatttgc ggcataatac cgatgacttc tccccctcct ttcagcaccg tatcggccag
    13201 gatctgcatc aatcccacat tggctccccc gtatacgagc cggatgttcc gctccagcag
    13261 cagcctgccc agctcctccg cagccttttt gtattcaggc tttttgccca tgctcgaccc
    13321 acaaaaaaca cagatggaat ccataccggt aattttcagc caaaataaag aatatttcca
    13381 aaactggaac tctggaacct ggaactctgg aatccttcgc ccgcctttgg cggatgaagg
    13441 acagggcccg gaactaattt aaacccattc taatcataac gatttcaaca tataatccaa
    13501 aaaacagtat ttttggcgct tcatcaaaaa ttccgtgaac gagctttacc ctttgaaatt
    13561 caagcctgta ttcgatgacc ggatctgggg aggtgaaaag atcaggacac acctgggtct
    13621 tgactttgcc cctttgaacc ggtgcgcgga ggcctggatc ctttccggtt acgaaggaaa
    13681 acagactgtt gtcagtaatg ggttcctgga gggcaatgag atcaatgaac tgatcgaaat
    13741 ctatatggat gacctggtcg gcgaaaaggt ttacgaaaat accggcgaaa catttccctt
    13801 gcttatcaag tttatcgatt cacgcgactg gctgtcggtg caggttcatc ccgacgacga
    13861 actggcacag aaaaggggga tgccgaacgg taaaacggag atgtggtacg tgatcgatgc
    13921 cgaagaaaat gcgcaactta tcgcgggctt taaccggaag atcactcaaa aacagtatct
    13981 tgaacatctt cataataaaa cccttccgga gatattgaat tacgaaaagg tgcggaaaga
    14041 cgacgcgatc ttcatgccgg ccggccggat ccactcattg ggcccgggcg tgctgctggc
    14101 cgagatccag caaacctccg acgcaaccta cagactgttc gactggcaca ggaaagattc
    14161 gcaaggtaag gaaagggagt tgcataccga actggccctt gcagctattg attttgatgc
    14221 attctcagac aataaaacga attacaagcc caggctgaac gaaacggtcg acctggtaag
    14281 ctgcccctat tttacgacaa acctgatcga attggcacag cctttgagaa agaactacga
    14341 ggagatcgat tcgttcgtgg tgtatgtctg cctggagggc gctatgagga taaaatgcgg
    14401 ggacagcccg acgacggtag ccaaaggtga agtggtcctg atcccggcga tgtttgacgt
    14461 ggtggagatc taccccgatg gtaatgtgaa gtttttagag gtttatattt aatttcaatc
    14521 agatatgaaa aaagtaatcg tattcttcat tttagttttc tcagggataa atctcctggc
    14581 ccaggaaggc atcctggaaa aaaccatccc tgccatcgac attaaaaccc tgcagggaga
    14641 aacggtcaac acctcttcca tcgataacca gggcaagccg atgatcatct ccttcttcgc
    14701 cctgtggtgc aaaccctgca tgcgtgagct caccgccatc tccgacgttt atgccgactg
    14761 gcaggaagaa attggagtta agcttgttgc catatcgatc gacgattccc gctcggtagc
    14821 caatgtattg cctactgtga acggaaatgg ctgggaatac gagttttaca gcgatgtgaa
    14881 cggcgatttc aaacgcgcta tgggcgtcaa tatgatccct catgtgtttg tggtcgacgg
    14941 caacaaga
//
LOCUS       DAOQXS010000008        13942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4859_length_13942_cov_3.329661, whole genome shotgun sequence.
ACCESSION   DAOQXS010000008 DAOQXS010000000
VERSION     DAOQXS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_13942_cov_3.329661"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="PKM34_00550"
     CDS             <1..2167
                     /locus_tag="PKM34_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNQ82095.1"
                     /translation="KWKTNIGAPLGEGNYTPAEAVYSRVKYTCDSIYVLDRDVELKVN
                     GTAYTYTTGCAVSILPSDPGQLCLYLVRRSESVEYLSVDRFLVNNGGILFKKNTGNIN
                     TSYWNLTIAGSSIEVSNDGKRIAINNRHQDGDWDDIFLIDAAIFDNSSDAVQGISIKE
                     LILQPDFNIVYSPASVDNIAANNPDLLFLKNIDRKISEIEFSPGGDYLYFVDGGFGAG
                     GLTNMTYLGQIYIGPVENPATYPFDIRLQIQKPPGTIDWDEGTGGAEANYPDTYFPTL
                     YLEKVFENKMYLIKKNSPYLFIIPYPDEPMNQLLSPGDIDFSDSNYPNILLHGKIWMT
                     PDQIEGYDYITSVDPVFDLGNDTILCPPGQIPLTPGDQFGSYCWQDGSTDAVYIATDT
                     GTYWVSVQDEFGCSYTDTIKIMTTAEGINLGPDFSICPGDTAVLNPGSGYFQYNWQDG
                     STMEEYSVYSPGTYWVEVTTITGCTATDTIVVDNNPLPEPDLGPDATICQGESITLDP
                     GAGFVQYLWHDGLSDRFYQAYQEGMIWVEVMNDKGCKSADTINITHIPFQQVDFGKDT
                     VITQNQTFILDAGDGYNMYEWQDGSSDRFYEVKDEGWYFVQASFENCISSDSVYVILN
                     DCQSTMIIPNCFTPNGDGYNEVFNVSATKISRFNMLIFNRWGQQLYESDDLEKGWDGK
                     VNGRVCPIGTYFYLIRYTTECVGGTEQPAEKKGSVTLLE"
     gene            2242..3228
                     /locus_tag="PKM34_00555"
     CDS             2242..3228
                     /locus_tag="PKM34_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ82096.1"
                     /translation="MDINRIVIIGAGAIGTALGNAMAKNDWNDITLISIEEDVVESIN
                     SGHINRKYFPGIILHPGLKATQSTDILARAEYIFMAIPSVETVKYLEIHREMVPANAI
                     IINLAKGFGEHNCTIIRCLQEILPNRICPMKGPTFARDIINNQPTAFTIGSENPQVFA
                     SFKKIFSNTNIYLDFSTDVMGVEILSILKNIYAIVAGITDASFDAPNLRSLVLTKAFR
                     EMRAILVEFGGQEQTMFTYSGFGDFTLTALNDLSRNRTLGLLIGKGFFTREMSDKVVL
                     EGKIAVNVLADVLASRGLSDKFPILMELHKVFTTHYDLSQFVDRIIKFNGEN"
     gene            complement(3433..6066)
                     /gene="alaS"
                     /locus_tag="PKM34_00560"
     CDS             complement(3433..6066)
                     /gene="alaS"
                     /locus_tag="PKM34_00560"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ82097.1"
                     /translation="MKSAEIRKIFLEFFSSKSHKIVPSAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGHAPAKHLRIADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWELLTDVYKIDKRNLYVTVFEGDAADNLPKDEEAALIWSRFVPADHILTGS
                     KKDNFWEMGETGPCGPCSEIHVDIRSEEEKAAVPGRELVNKGNPLVIEIWNLVFIEFN
                     RQMDGKLVPLPQKHVDTGMGFERLSMVIQGKKSNYDSDIFQPVIGEIAKLSGRVYGQN
                     EKEDIAMRVIADHLRAISFAIADGQLPSNTGAGYVIRRILRRAVRYGFTFLDMKEPFI
                     YRLVPVLALHMGEYFPELYSQGDLVRKVVAEEEQSFLRTLSHGIRRFEEYVKENPGRN
                     EIEGKFAFELFDTYGFPVDLTQLMASEQGLYVDMPGFQAGLEEQKNRSRQAAQVDTHD
                     WTVIKPDGEETEFIGYDTLDAEVEVVKFRKVRAKGQEYVEIVLDRTPFYAESGGQVGD
                     RGYLESDGHRIMIEDTYKENELIIHKASEAPGSTGKPYRAVVDNEKRRATANNHSATH
                     LMHAALRTVLGSHVEQKGSLVDEDHLRFDFSHFAKMSDEEIRRVEEIVNRKIRENIEK
                     SEERSVPVEEAKNRGAMALFGEKYGDEVRVITFDPDYSIELCGGTHVHSTGQIGLFKI
                     VSEGAIAAGVRRIEAVTGEKAEAWFYEQANTVTAVRELLKNAKDVVKGVKNLLDENKD
                     LQHRIEELNRYRIQALKSELYDNAEVIVDTRFIAASLDIDPKSAKDLSFMLHAMPGSM
                     FILVFTTGGEKVSMSLMLTEDLVKGKGLHAGNMVKELAREIGGGGGGQPHYATAGGTH
                     ANGIKSAIEKARAMLTDSQGR"
     gene            6201..7175
                     /locus_tag="PKM34_00565"
     CDS             6201..7175
                     /locus_tag="PKM34_00565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ82098.1"
                     /translation="MGKKVKYVFNHKTLSFEHARLTARHYILKVLSYLSTSVAFTAVI
                     VLIGTYFFDSPKERMLKREIRQYELQFKLINERMAKLETVLNDMADRDDNIYRVIFES
                     EPIPTEARKAGIGGSDRYRDLEGYRNSEILVKTARKLDQITAKMYVQSKSFDEVYEMA
                     INKSRLLAGIPAIQPVSNQDLRRLSSYFGWRTDPFYKVQKFHEGVDFSAPPGTEIYAT
                     GDGKVLQADRSKTGYGNKIVIDHGFGYKTMYAHLQSFKVRAGETVKRGQVIGTLGSTG
                     KSTSPHLHYEVWKNNTPVNPINYFFNDITPEQYEEMLILSQRPSQTMD"
     gene            7187..7504
                     /locus_tag="PKM34_00570"
     CDS             7187..7504
                     /locus_tag="PKM34_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ82099.1"
                     /translation="MAEKLYYSIGEVAEMFQVNASLIRFWEKEFPIIHPQKNRKGNRM
                     FTSGDIDHLRIIYNLVKERGFTIQGARDYLSKNRSEAEKNHEVVKSLQNIRSLLVRLG
                     DEL"
     gene            complement(7520..8491)
                     /gene="rfaD"
                     /locus_tag="PKM34_00575"
     CDS             complement(7520..8491)
                     /gene="rfaD"
                     /locus_tag="PKM34_00575"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNQ82100.1"
                     /translation="MGKIVITGAAGFIGSCMAGKLNHKGYREIVLVDNFTNPRKQKNY
                     CHKSIAEKVERDDFFAWLKQHADEVEFVFHLGARTDTTETNPEVFDRLNLGFTRDVWK
                     LCTEQRISLIYASSAATYGGGEHGYSDDHSRVELYKPLNLYGESKHEFDKWALAQKAS
                     PPYWAGMKFFNVYGPNEYHKGRMASVIFHAFHQIRETGRVRLFRSHRPDFKDGGQLRD
                     FIFVRDVVDVLFFLMKRGGHSGIYNLGTGKARTFLDLVRAVFHALDKPENIEFIDTPE
                     DIRDKYQYFTEAPMAKLRAEGFEEDFYSLEDGIEEYVVEFLMNDRYF"
     gene            8655..9209
                     /locus_tag="PKM34_00580"
     CDS             8655..9209
                     /locus_tag="PKM34_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82101.1"
                     /translation="MKMNCLRFGLLLLPTLFLTAFTSFAQVSEGGLPPSFSLQGLSSQ
                     VDRLVYPAPDMEILMAEDEQNLRSAFPGPERMAYSVPVNLDVRKAGTVEILADGSILR
                     RMAVYIPGALALGVYYSDFHLPDGAKLFLYNEAQTQVIGAFTNANNHESRLFANEFIQ
                     GDWSVSNTWNRPEQAATYPSLYQK"
     gene            9272..11146
                     /locus_tag="PKM34_00585"
     CDS             9272..11146
                     /locus_tag="PKM34_00585"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82102.1"
                     /translation="MINVKCPEGNGWENQIQGVARLSIKIGWSYYWCSGSLINNTNND
                     RTPYLLTAEHCGDGANASDRNQWVFYFNYQSATCTGNYGPSSNTMTGCTLKANDPNDN
                     EYLGADFELVKLNGTPPSGYNVYYNGWNRTNSPADSGVCLHHPAGDIKKVSTYQNMVS
                     STWWNGTPSHWKVTWIETESGLSIMQGGSSGSSVFDENGLIMGDLSGGYSSNSCTNPS
                     PAWFGKIWYAWDQNGDVPAERLKDWLDPTNTGVYTHPGLSSQILPPVVDFEADTTYLL
                     QGESVHFTDLTTGNPALSWEWSFPGGTPNASDIQNPTITYNQAGTFDVTLTVTNADGE
                     ETELKPGYITVEQVLLPDADFVASALEVTEGDMIDFTDLSTNNPTAWDWIFNGGDPAT
                     SGEQNPDSVIYAVPGVYNVYLTASNNGGSNTELKENYITVLAGAPPVSDFYADKTEIL
                     PGDTVNFFDLSTGNPTQWTWTFEGAATGTSSVQNPENIVYPTEGTFYVRLRTKNFFGN
                     NTIQKDGYITVGNISVGEINRQQGVMAFPNPTAGLLTVKLPEGAEAWTGRPVQGGNIQ
                     SSVNIEIVNSLGNVIRKAEFHADDQGVTFDLTDEPSGLYFVRVSTPTRSVQKKISLLR
                     "
     gene            11174..13102
                     /locus_tag="PKM34_00590"
     CDS             11174..13102
                     /locus_tag="PKM34_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82103.1"
                     /translation="MLKHLRQGDRKDSPCLPFSHSPHLLITLIALFPLLLFSQISQKG
                     TPESFRYNLPAAREHILTMPERSILDAEDQESEKNGLNRRIGIAVFAGLDVMNDGDVF
                     LTDEGTQIWRLEVTCSGALAIGLHFDRFFLPEGFSLYAYSPGKNSVLGAYTLYNNKPQ
                     GIFSTELTTGDRVIIELNAIAGADISGARCHLGEISCVYRDLPDRIAGRSSSDDCEVN
                     INCPEGDNWQFQKQGVVRIYVRDGGGFFWCTGSLVNNTRQDNTPYLLTADHCAPYVTP
                     EELAQWIFYFNYEYPGCENISQTPELLTMNGAAKLANYDTSGSDFLLLRLDNEVPPHY
                     DPFFNGWNIENSASFSGVTIHHPAGDVKKISTYTDQLTSSQWSGEFGTHWQVYWSATE
                     SGWGVTEGGSSGSPLFDNYGRIIGTLTGGQAACEPGGGGPNTGPDQPDFYGKFSYSWD
                     QNGTDSSQQLKYWLDPDNTGISFLPGKFTQLTAAFNADPVITLIGNDVIFTDYSSGDP
                     MAWEWYFEGGDPETYNGDGPVTVNYPNGGKFDVRLTVTDGIAYDTLYLKDYIHIVGKV
                     YPNPTDGLVSIYITEELPSDIKAEVFTIPGLKVFEQTFTEQTYPLITLDLSGYSSGLY
                     LIRLTIKQRYLFVKVIVR"
     gene            complement(13165..13365)
                     /locus_tag="PKM34_00595"
     CDS             complement(13165..13365)
                     /locus_tag="PKM34_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82104.1"
                     /translation="MGLLIIVPVIIVSASAPPPPPPGPGGTDDPIGGGAAPIGSGIVM
                     LISMGAAYGAKKVYDARKRLEE"
     gene            complement(13443..>13942)
                     /locus_tag="PKM34_00600"
     CDS             complement(13443..>13942)
                     /locus_tag="PKM34_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82105.1"
                     /translation="GTEVVSVPFSFKCGADGTYSIEAANTSSFDVGSEIFLEDLLTGA
                     DWHNLMVNPVYEFNGSASDVPERFILHFFGPTSVDDPDDEMADVKDVRIYGYGHDAYI
                     VNFGSETVKEFVAYDLMGRELHRGTLPNNSVNKVQIGDVSAYYIVKVITKEGNVYTGK
                     VYITK"
BASE COUNT         3540 a         3652 c         3178 g         3572 t
ORIGIN      
        1 taagtggaaa accaatatcg gcgcgcctct cggtgaagga aactacacgc ctgccgaagc
       61 ggtctattca cgggtgaagt atacctgcga cagcatctat gtcctggata gggatgttga
      121 attaaaggtc aacggcactg cgtatactta cacgacagga tgtgcagtca gtattttacc
      181 ctctgatccc ggacaactat gcctctacct cgtacgcagg agtgaatcag tggaatacct
      241 gtcagttgac cgtttcctgg ttaacaatgg cggaatccta tttaagaaga acaccggtaa
      301 tattaatacc tcttattgga acctgacaat tgccggttca tcaatcgaag tctcgaatga
      361 cggaaaacgc attgcaatta ataaccggca ccaggatggc gactgggatg atatcttcct
      421 catcgatgcg gcaatattcg ataattcatc agatgctgtg cagggaattt ccataaagga
      481 gctgatcctt caacccgatt ttaacatagt ttattctccc gcatctgttg ataatattgc
      541 tgccaataac cctgatttac tgttcctcaa aaatattgac cgtaaaatct cagaaattga
      601 attctcaccc ggaggagatt atctgtattt cgtcgatggc ggtttcggag ccggcggatt
      661 gacaaatatg acctacctgg ggcagattta tatcggaccc gtcgaaaatc ctgcaactta
      721 tccattcgac ataaggcttc aaattcaaaa accaccagga acaatcgatt gggatgaagg
      781 aaccggtggt gctgaagcga attatcctga tacttacttt cctactttgt atttggaaaa
      841 ggtttttgaa aacaaaatgt acctgataaa gaaaaactca ccatacctct ttatcattcc
      901 atatcctgat gaaccaatga accagttgct gtcgcccggt gatatcgatt tttccgacag
      961 taattatccg aatatattat tacacggaaa aatatggatg acgcctgacc agattgaagg
     1021 gtacgattat attacttccg tagatccggt attcgacctt ggaaatgata cgatcttatg
     1081 ccctcccggc cagatcccgc ttactccggg cgatcagttc ggatcctatt gctggcagga
     1141 tggctccacg gatgcagtgt atatcgccac agatacaggg acatactggg tctcggtcca
     1201 ggatgaattt ggttgcagct ataccgatac cattaaaatt atgacaaccg ccgaagggat
     1261 aaaccttggc ccggattttt caatatgccc cggcgacaca gcagtattaa atccaggcag
     1321 cggttatttt caatataatt ggcaggatgg atcaacaatg gaagaatatt ccgtatattc
     1381 tccgggtact tattgggtgg aagtcacaac aataacaggt tgcaccgcga ctgatacaat
     1441 agtggtcgat aacaatcctc tacctgaacc cgatttgggg ccggatgcca ccatatgcca
     1501 gggagaatcc ataacgttgg atccaggggc cggtttcgtc cagtacttat ggcatgatgg
     1561 cctttctgac aggttctatc aggcctacca ggaagggatg atttgggttg aagtaatgaa
     1621 tgataaagga tgcaaatcag cagatacgat aaatatcacc cacatcccat ttcaacaggt
     1681 tgattttgga aaagatacgg tcattaccca aaatcagaca ttcatactcg atgcaggtga
     1741 tggatataat atgtatgaat ggcaggatgg atcatcagac cggttttatg aagtgaagga
     1801 tgaagggtgg tacttcgtac aggcttcatt tgagaattgt atttcttcag atagtgttta
     1861 tgtgattcta aacgattgtc agtctacaat gatcatccct aattgtttca caccgaatgg
     1921 ggatggatat aacgaggttt tcaacgtgtc ggccacaaag atctcaagat ttaatatgct
     1981 cattttcaac aggtgggggc agcaattata tgaatcggat gacctggaaa aaggttggga
     2041 tggaaaggtt aacggacgag tgtgtcctat tggcacttac ttttatctga tcagatatac
     2101 taccgaatgt gtcggcggta cagaacaacc tgcggagaag aaaggttcag tgactctgct
     2161 tgaataattc agaccattta acattcgctt cggccaatca ttttattcat ttatttttgc
     2221 cggatcaata ttactattgt aatggatatt aacaggatcg taatcatcgg cgccggagcc
     2281 atcggaaccg ccctgggcaa tgcgatggca aaaaatgact ggaacgatat caccttgatt
     2341 tctattgagg aagatgttgt tgaatccatc aactccggtc atatcaaccg caaatatttt
     2401 cccggcatta tccttcatcc cggattaaaa gcgacccaaa gcaccgatat tcttgcacgg
     2461 gccgaatata ttttcatggc tatcccctcc gtagaaacgg taaagtacct tgaaatccat
     2521 cgcgagatgg tgcctgcaaa tgccatcatc atcaacctgg caaaaggttt cggggaacat
     2581 aactgcacca ttatccgctg cctgcaggag atccttccca accgcatctg cccgatgaaa
     2641 ggccccacct tcgcccgcga tatcatcaac aaccagccca ccgcttttac tatcggctcc
     2701 gaaaaccctc aagtattcgc atcgtttaaa aagatattca gcaataccaa tatttacctc
     2761 gacttcagca ccgatgtgat gggtgtggaa atcctcagca tcctgaaaaa tatttacgca
     2821 attgtggccg ggatcaccga cgccagcttc gatgcgccga accttcgctc actggtgctc
     2881 accaaagctt tccgggaaat gcgggcaatc ctggttgagt tcggcggaca ggaacaaacc
     2941 atgttcactt acagcggttt cggcgatttc accctgacgg cgctgaacga cctcagccgg
     3001 aaccgcactc tgggattgct catcggaaaa ggattcttta cccgcgaaat gtcggacaag
     3061 gtggtcctgg aagggaaaat agcggttaat gtactggctg atgtcctcgc cagcaggggg
     3121 cttagcgaca aattccccat cctgatggag cttcacaaag tctttactac tcattacgac
     3181 ctatcccagt tcgtcgacag gatcataaaa ttcaacgggg aaaattaaaa caacgaaccg
     3241 acggctttag tcgtcggagc acaaaccccg taggttgtct caaaaaggtt taaccacgaa
     3301 ggcgcacaaa ggattacacg aaggaacaca aagatatcct cctgttaatc aattacttcg
     3361 tgcgccttcg tgaagattct ttgtgcgcct tcgtggttaa ataaaagact tttaagacga
     3421 cctcttttat ttttaacggc cctggctatc ggtaagcata gccctggctt tctcaatggc
     3481 tgatttaatc ccgttggcat gggtaccgcc ggccgtagcg taatgcggct gtccgccgcc
     3541 gccgccgccg atctctctgg ccagctcctt caccatattg ccggcgtgaa ggcctttccc
     3601 tttgacaagg tcttctgtca gcatcaggct catgctgacc ttttcaccac ccgttgtaaa
     3661 taccaggatg aacatgcttc cgggcatggc gtggagcatg aacgacaggt ctttggccga
     3721 tttgggatca atatccaggc tggcagctat aaaccgggtg tcgacgatca cttctgcatt
     3781 atcgtacaat tcactcttca aagcctggat cctgtaccgg ttcagttctt cgatccggtg
     3841 ctggagatct ttgttttcgt ccaggaggtt tttcacccct tttactacat ctttggcgtt
     3901 cttaagaagt tctctgacgg ccgtcaccgt attggcctgc tcgtagaacc aggcttcggc
     3961 cttttcaccg gttaccgcct cgatacggcg cactccggct gcgatggccc cttccgaaac
     4021 gatcttgaac aaaccgatct gtccggtgct gtgaacatgt gtccccccgc acaattcaat
     4081 cgaataatcc ggatcaaacg ttatcaccct cacttcgtcg ccgtattttt ctccgaaaag
     4141 ggccatcgcc ccccggttct ttgcctcttc aacgggcact gaccgctctt cgctcttttc
     4201 aatattttca cggatcttcc ggttaacgat ctcttccacc ctgcgaattt cttcatccga
     4261 cattttggca aaatgggaaa aatcgaaccg caaatgatcc tcatccacca gggagccttt
     4321 ttgctcaaca tggcttccca gaaccgtccg caatgcagcg tgcatcaggt gtgtcgcgct
     4381 gtgattgttg gccgttgctc tccttttttc gttatcgacc acagcccggt aaggttttcc
     4441 ggttgacccc ggagcctctg atgctttgtg aatgatcagc tcattctcct tgtaggtatc
     4501 ttctatcatg atcctgtggc cgtcagattc caggtagccc ctgtctccaa cctgccctcc
     4561 cgattccgcg taaaaaggcg tccggtcgag aacaatttcg acatactcct gcccttttgc
     4621 cctcaccttg cggaatttga ccacctcgac ctctgcatcc agtgtgtcat atccgataaa
     4681 ttcggtctct tcaccatccg gttttattac ggtccagtcg tgtgtatcta cctgcgcggc
     4741 ctgccgggag cggttctttt gttcttccag ccccgcctgg aaacccggca tgtccacata
     4801 taaaccctgc tctgaagcca tgagctgtgt caggtcgacc gggaaaccgt acgtatcgaa
     4861 caattcaaaa gcaaattttc cttcaatctc attacgtccc gggttttctt ttacatattc
     4921 ctcaaacctg cgaataccat gcgacagggt ccggaggaat gactgttcct cctctgccac
     4981 cactttgcgg accaggtcgc cctgtgaata gagttccggg aaatattcac ccatatgtaa
     5041 ggcaagaacc ggaacgagcc ggtaaataaa cggctccttc atgtccagga aagtaaaacc
     5101 gtacctgacc gcccttctta atatccttcg gatcacatac ccggcgccgg tattcgacgg
     5161 caactgcccg tcggcaatag caaaggagat cgcccgcaag tggtcggcga tcacccgcat
     5221 agcaatgtct tccttctcat tttgcccgta tacccttcct gaaagtttgg caatttctcc
     5281 gataaccggc tggaaaatat cggaatcata attcgacttt ttcccctgga tgaccatcga
     5341 cagccgttca aatcccatcc cggtatcgac atgcttctgc ggaagcggga ccagctttcc
     5401 gtccatctgc cggttaaact cgataaaaac caggttccag atctcaatga caagcggatt
     5461 gcctttattg accagctctc ttccgggcac cgccgctttt tcttcttcgc tgcggatatc
     5521 cacatgaatt tccgagcagg ggccgcaggg gcctgtctcc cccatctccc agaaattatc
     5581 ctttttggat cctgttagga tatgatcagc aggcacaaac cggctccaga tcaaagcagc
     5641 ctcttcatct ttcggcaggt tatcagcagc atctccttca aaaacagtca cataaagatt
     5701 cctcttatca atcttataaa catccgtcag aagttcccat gcccattcaa tcgcctcttt
     5761 cttgaaataa tccccgaacg accagttgcc cagcatctca aacatggtgt ggtggtaggt
     5821 gtcgtgaccg acctcttcga ggtcattatg ctttcccgat acacgcagac atttctgtgt
     5881 atcggctatt ctcaaatgct ttgccggggc atgcccgaga aaaatatcct tgaactggtt
     5941 catccctgca ttggtaaaca tcagggtcgg atcgttctta acgaccattg gagccgacgg
     6001 aacaatctta tgtgatttcg aagagaaaaa ttccaaaaat atctttctta tttctgctga
     6061 tttcattaat tttctgatta tttgcctgtt aatacgcttt taaaaacgaa ctgcaaaatt
     6121 aattttattt atttttgcac tccgttcaaa tacggaaaat ggaagaatat tcgttcatac
     6181 ctgcggaagc cattgattag atgggaaaga aagtcaaata cgtattcaac cataaaacat
     6241 tgtcgttcga gcatgcccgg ctcactgccc ggcattatat cctcaaagtc ctttcctatc
     6301 tgtccaccag tgtggccttc acggccgtga tcgtcctgat cggtacctat ttttttgatt
     6361 ctccgaaaga aaggatgctc aagcgtgaga tccggcagta tgaacttcag tttaaactga
     6421 tcaatgagcg gatggcgaag cttgaaaccg tgctgaatga tatggccgac cgcgacgata
     6481 acatctaccg cgtcatcttc gaatcggaac cgattccgac ggaagcacgc aaagcgggta
     6541 tcggcggttc ggaccggtac agggacctgg aaggctacag aaattcggaa atcctggtaa
     6601 aaacggcccg gaagcttgac cagatcacgg cgaaaatgta cgttcagtcg aagtcatttg
     6661 atgaggttta cgaaatggcc atcaacaaat caaggctgct ggccggcatt cctgcgattc
     6721 aaccggtcag caaccaggat cttcgccggt tatcctctta ttttggctgg aggaccgatc
     6781 ctttctacaa agtgcagaag tttcacgaag gcgttgattt ttcggctcct ccggggaccg
     6841 aaatctatgc tacgggcgat ggcaaggtgc tccaggccga ccgttccaaa acgggctacg
     6901 gtaacaagat cgtgatcgat cacggtttcg ggtataaaac catgtatgcg cacctgcaat
     6961 cgttcaaggt cagggccggc gaaaccgtta aaagaggcca ggttatcggc accctgggaa
     7021 gtaccggaaa atcgacatca ccacacctcc attacgaggt ctggaaaaat aacacaccgg
     7081 tcaatccgat caattacttt ttcaacgaca taactcccga gcagtacgaa gagatgctga
     7141 tcctctccca gcggccttct caaaccatgg attaaaatca atcgcattgg ctgaaaaact
     7201 atattatagc atcggtgaag tagcagaaat gttccaggtg aacgcatccc ttatccgttt
     7261 ctgggaaaag gaatttccca tcatccatcc gcagaagaac cggaaaggaa accgtatgtt
     7321 tacttccggt gatatcgatc atttgcggat aatctacaac ctggtgaaag aaagaggttt
     7381 tacgatccag ggggcacgcg actacctgtc aaaaaacagg agcgaagccg aaaaaaacca
     7441 tgaagtggta aaatcgcttc agaatatccg gtcgctgctg gtcaggctcg gcgacgaact
     7501 ttgatcaata ctgcttccat cagaaatacc ggtcgttcat aaggaactcg accacatact
     7561 cttcaatgcc atcctccagc gaataaaaat cctcttcaaa gccctccgcc cgcaatttag
     7621 ccatcggtgc ttccgtgaaa tactggtatt tatcccggat atcttccggt gtatcaatga
     7681 attcgatatt ttccggttta tccagggcat gaaataccgc ccggacaaga tccaggaatg
     7741 tgcgtgcctt tccggtcccc aggttataaa ttccgctgtg cccaccccgt ttcatcagga
     7801 aaaataatac atccaccaca tccctgacaa agataaaatc ccgtaactgg cctccatcct
     7861 tgaaatcggg acggtgtgaa cggaacagcc tgaccctgcc ggtctccctg atctggtgaa
     7921 aagcatggaa tatcaccgaa gccatcctcc ccttatggta ctcgttcgga ccgtaaacgt
     7981 tgaagaactt cattcctgcc cagtaaggag gcgacgcttt ctgcgccaat gcccatttat
     8041 caaattcatg cttcgattct ccatacagat tcagcggttt atacagttcc acccggctgt
     8101 ggtcatcgct gtatccatgc tcacccccgc catatgtagc agcagatgaa gcgtagatta
     8161 aactgatcct ctgctctgtg cataacttcc agacatccct ggtaaatccc aggtttaacc
     8221 ggtcaaaaac ctccggattc gtctccgttg tatcggtcct ggcgccaaga tgaaacacaa
     8281 attccacctc atccgcatgc tgtttcagcc aggcgaagaa gtcatcgcgc tcaacttttt
     8341 cagcaattga tttatgacaa tagttctttt gttttcgtgg atttgtaaaa ttgtcaacca
     8401 gaacaatctc ccggtagcct ttgtggttga gttttccggc catgcaactc ccaataaaac
     8461 ctgctgcgcc agtgatgact attttgccca tatccgtgaa atcattgttt aatcaaatat
     8521 acgattattc attcctttaa ttgctcaccg gaatcaattt tttacttccg tctcacctga
     8581 ttttatctat atttgtcatc gttaacccgg atgattataa tgatcttctt acaataatta
     8641 ttaatttttt tacgatgaaa atgaattgtt tacgctttgg tttgcttctt ttgccaaccc
     8701 tgttccttac agcttttact tcattcgccc aggtaagcga aggtggcctt cccccgagtt
     8761 tctcgcttca ggggctttct tcacaggtcg atcgcctggt ctatccggcg cctgatatgg
     8821 aaatattgat ggcagaagat gagcaaaatt tacggagcgc ctttcccggc cccgagcgta
     8881 tggcctattc ggtgcctgtc aatctcgatg tcagaaaggc cggcaccgtt gaaatcctgg
     8941 ctgatggttc catacttcgc cggatggcgg tctatatccc gggagcgctg gcacttggag
     9001 tctactacag tgattttcac ctgccggatg gcgcaaaact cttcctttat aatgaagcgc
     9061 aaacccaggt catcggagcc ttcacgaatg ccaataacca cgaatcgcgc ctctttgcca
     9121 acgaattcat ccagggcgac tggtcagtat cgaatacctg gaaccggccg gaacaagcgg
     9181 cgacgtatcc gtcattatat cagaaatagc ttatgcttat cgttatatcg attttgttga
     9241 aaatggaaac caggatcagt catggtcctg catgatcaat gtcaaatgcc cggaaggcaa
     9301 tggctgggaa aatcagatcc agggagttgc ccggctttcg atcaagatcg gctggagtta
     9361 ttactggtgc tccggttcac tgatcaacaa cacaaacaac gacagaaccc cctacctgct
     9421 cacggctgaa cactgcggcg acggtgccaa tgcctccgac cgcaaccagt gggttttcta
     9481 cttcaattac cagtcggcta cctgcaccgg caattatggc ccttcgagca ataccatgac
     9541 cggttgcacg ctgaaagcca acgatcccaa cgacaatgaa taccttggcg ccgatttcga
     9601 actggtgaaa ctgaacggga ctcctccctc gggctacaat gtttattaca acgggtggaa
     9661 ccgtaccaac agcccggccg acagcggcgt ttgcctgcat caccccgccg gtgatataaa
     9721 aaaggtttcc acttatcaga atatggtctc ttccacctgg tggaacggca ccccgtcgca
     9781 ttggaaagtc acctggattg aaaccgagag cggactgtca atcatgcagg gcggctcttc
     9841 cggctcgtcc gtattcgacg aaaatggcct gatcatgggc gacctgtcgg gcggttattc
     9901 ttccaattcc tgcactaacc cgtctccggc atggttcggg aaaatctggt acgcatggga
     9961 ccagaacggt gatgtcccgg cagaacgcct gaaagactgg ctcgacccga ccaataccgg
    10021 tgtttatacc caccccggcc tttcgtcgca aatactcccc ccggttgttg attttgaagc
    10081 agatacaacc tacctcctgc aaggggaaag tgtccacttt accgacctga ccaccggaaa
    10141 ccccgccctc tcctgggaat ggtcgtttcc cggcggtacg ccgaatgcct ccgatatcca
    10201 gaacccgact attacctata atcaagccgg cacattcgat gtaaccctga ccgttaccaa
    10261 tgccgatggc gaagaaacgg aactgaaacc cgggtatatc acggtcgaac aggtcctcct
    10321 gccggatgct gacttcgtgg ccagcgccct cgaagtgacc gaaggcgata tgatcgactt
    10381 taccgacctg tcgaccaaca acccgacagc ctgggactgg atcttcaatg gcggcgatcc
    10441 ggccacatcc ggcgaacaga atcctgactc ggtcatctat gcagtccccg gtgtttacaa
    10501 cgtctatctg accgcatcga ataacggggg atccaacacc gaactcaaag aaaactatat
    10561 cacggtactt gcaggcgctc cgccggtttc cgacttctat gccgataaaa cggaaatcct
    10621 tcccggcgac acggtgaatt tcttcgatct ctccaccgga aatcccaccc aatggacctg
    10681 gacctttgaa ggcgcagcaa ctggaacttc cagtgtgcag aacccggaaa acatcgtcta
    10741 tccaactgaa ggcacattct atgtaaggct gaggaccaag aacttctttg gcaacaatac
    10801 gatccagaag gacggttata tcaccgtcgg caacatctcg gtcggtgaga tcaaccgcca
    10861 gcagggtgtc atggcatttc ccaacccaac ggccggtttg ttgaccgtta aactgccgga
    10921 aggtgcggaa gcctggaccg gacgaccggt acaaggtggc aacattcaat cctctgtaaa
    10981 catagaaatc gtcaattcgc tcggaaatgt catccgcaaa gcggaatttc atgctgatga
    11041 ccagggggtt acattcgatc tgaccgatga gccgtcaggt ttatattttg tccgtgtttc
    11101 tacccccacc cgctcggtac agaagaagat ttcacttctg agataattct caatgtcaat
    11161 atctttaatt actttgctta agcatttaag gcagggagac cgcaaggatt ctccctgtct
    11221 ccccttctcc cattctcccc atctccttat cacccttatc gccctttttc cccttctcct
    11281 tttctcacaa atttcgcaaa aaggcacgcc tgaaagtttc aggtacaatc tcccggcagc
    11341 cagggagcat atcctgacca tgcccgaaag aagcatcctg gatgctgaag atcaggaatc
    11401 ggaaaagaat ggcctgaacc ggcgaattgg gatcgcggtc tttgccggac tggatgttat
    11461 gaacgacggc gatgttttcc ttacggatga gggtacccaa atctggcgac tggaggtaac
    11521 ctgcagcggg gcactggcca tcgggctaca cttcgaccgg tttttcctgc cggaaggatt
    11581 tagtctttat gcttacagtc ccggcaaaaa ttcggtgctt ggggcttata ccttatataa
    11641 taataaacca cagggcattt tttcgaccga gctgaccacc ggagatagag tgatcatcga
    11701 actcaatgcc attgccgggg cagatatttc cggtgcccga tgccatctcg gggagatatc
    11761 atgcgtttac cgcgatttac ccgacagaat cgccggccgg agttcatcgg acgactgcga
    11821 ggtgaatatc aattgcccgg agggtgataa ctggcagttt cagaagcagg gagtggtccg
    11881 gatctatgtc agggatggcg gcggtttttt ttggtgtacc ggttcgctgg tcaacaacac
    11941 ccggcaggac aatacgcctt atctgctgac ggcagatcat tgtgcgcctt atgtgacccc
    12001 ggaagaactg gcacaatgga tcttttattt caattatgaa tatccgggat gtgagaacat
    12061 ttcccagacg cctgagctgc tgaccatgaa tggcgcagca aagctggcca attacgacac
    12121 atccggttcc gatttcctcc ttctccggct tgataatgag gtaccaccgc attacgatcc
    12181 tttttttaac ggctggaaca ttgagaattc ggccagcttc agcggggtca ccatacacca
    12241 cccggcagga gatgtgaaaa agatttcgac ctataccgac cagcttacct cctcgcagtg
    12301 gagcggtgaa ttcggcacgc actggcaggt ttactggagc gctaccgaaa gcggctgggg
    12361 tgtgaccgaa ggcggatcgt cgggatcgcc gctgttcgac aattacggcc gtatcatcgg
    12421 caccctcacc ggtgggcagg cggcctgcga accgggtggc ggcggaccga atacaggccc
    12481 cgatcaaccc gacttctacg gcaagttctc atactcctgg gaccagaacg gcaccgattc
    12541 ctctcagcaa ctaaaatact ggctggatcc tgataacacg ggcatttcat tccttccggg
    12601 aaaattcacc cagctaacgg ccgcattcaa cgccgatccg gtaataacat tgattggcaa
    12661 cgacgtcatt ttcaccgatt acagttccgg cgatccgatg gcctgggagt ggtatttcga
    12721 gggaggcgat ccggaaacct acaacgggga tggccctgtt acggtcaatt accccaatgg
    12781 cggcaagttt gatgtaaggc tgacggtaac cgacggtata gcgtacgaca ctttgtacct
    12841 aaaggattac atccatattg tcggaaaagt gtatcccaat cccaccgacg ggctggtgag
    12901 catctacatt accgaggaac tgccctcgga tattaaagca gaggttttta cgattcccgg
    12961 gttgaaggtg tttgagcaga cattcacaga gcagacctat ccattgatca ccctagacct
    13021 ttccggttat tcatcgggcc tttaccttat ccgtctgacg ataaagcagc gttatttgtt
    13081 cgtcaaagtc attgtgcgat aagcaacaaa aagagggcga tctcaacaga gacgccctcg
    13141 tattatacaa atgactttgg gtccttattc ttcgagcctt ttccgggcgt cgtagacttt
    13201 ctttgcgccg taggctgctc ccattgagat cagcatcacg ataccactgc cgatgggagc
    13261 tgctccgccg ccgatggggt cgtcggttcc tcccggtcct ggggggggag gtgggggggc
    13321 tgaggcgctg acgatgatca caggaacaat tatcaataat cccacgagaa tcaggttaac
    13381 tattgctttt ttcatatcca gaattgtttt ttttcgttaa ttatattaat aattgtccgt
    13441 tcttacttcg tgatatacac cttaccggta tagacattgc cttctttggt aatgaccttc
    13501 acgatatagt aggcagaaac atcgccgatc tgcaccttgt tgaccgagtt gttgggcagg
    13561 gtaccgcgat gcagttcacg tcccatcagg tcataggcca caaattcttt caccgtttcg
    13621 ctgccgaagt tgacgatata ggcatcgtgc ccgtaaccgt agattctgac atctttcaca
    13681 tcggccattt catcgtccgg atcatcgact gaggtcggcc cgaagaagtg gaggatgaat
    13741 ctttccggaa catcacttgc tgaaccgtta aactcgtata ccgggtttac catcaggttg
    13801 tgccagtcgg cgcctgtgag caagtcttca aggaaaatct cgctacccac atcaaatgat
    13861 gacgtattgg cggcttcgat agagtaagta ccatcggcgc cacatttgaa cgaaaatggt
    13921 acggagacca cttcggttcc ta
//
LOCUS       DAOQXS010000009        13864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4896_length_13864_cov_4.094286, whole genome shotgun sequence.
ACCESSION   DAOQXS010000009 DAOQXS010000000
VERSION     DAOQXS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4896_length_13864_cov_4.094286"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKM34_00605"
     CDS             complement(<1..792)
                     /locus_tag="PKM34_00605"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNQ82106.1"
                     /translation="MKRFTIFFSLFILLSSPSLRADEGMWLPIFVERLNYVDMQKMGL
                     HLTAEEIYSINNSSLKDAIVGLGNGSQPGGFFCTAEVVSSQGLLFTNHHCGYDIIQKH
                     SSIEHDYLNDGFWAMSKEEELPNEDLSATFFVRMEDVTDSIVPFLSDTMSASKRSEKV
                     REITARLKKAANPDKKYFVNVTSFFNGNEYYLFVYEVYTDVRLVGAPPSAIGKFGGDT
                     DNWMWPRHTGDFSIFRVYTAPDGSPAEYSKENVPMVPKHHLPVTLN"
     gene            complement(897..2594)
                     /locus_tag="PKM34_00610"
     CDS             complement(897..2594)
                     /locus_tag="PKM34_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HNQ82107.1"
                     /translation="MLNNVFSKILLVVTVIIAVSCQQPQKTEFQMEQQYPGTVTGQQP
                     LAPASPEHVANRLATFAPFTLTTDISHLTANEKQIVAIFFDVADIMDELFWLQAMGPK
                     EPFLARIQDETTKQFARINYGPWDRLDGNNSFIDGIGEKPAGANFYPADMTKEEFEAF
                     SDPNKKSLYTLIRRNADGSLRSVWYHQAYETQLKKAAELIKQAAALAEDAGLKKYLEL
                     RADALLTSDYQPSDFAWMEMRNSALDFVVGPIENYEDGLYGYKAAFESFILVKDPVWS
                     ARLVKYAAMLPDLQRGLPVREEYKTEIPGADADLNAYDVVYYAGDCNAGSKTIAINLP
                     NDEEVQLQKGSRKLQLKNAMQAKFDKIMVPIAELLIDPAQRKHVTFNAFFENTMFHEV
                     AHAIGVKNTINGKGPARTALKETYSSIEEGKADIMGLYLVTKLYEMGELTEGEVMDNY
                     VTFFAGIFRSSRFGAASAHGKANMMRFNFFRSEGAFTRSEEGIYTVDFDKTKAAMEKL
                     VQKIIYIQGDGDYEAAKNWVATEGIVTPALQTDLDRINASGIPVDVVFEKGKQMLGL"
     gene            complement(2687..3778)
                     /locus_tag="PKM34_00615"
     CDS             complement(2687..3778)
                     /locus_tag="PKM34_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ82108.1"
                     /translation="MKKALKILAGVIVLSIFVYTIYFLYQKSQAKPVVFETARAFKTE
                     IIKSTVATGSVVPRKEIMIKPQVSGIVEEVYVEAGDMIRKGDLIARIKIIPDMVTLNN
                     AQSRVNRAKIGLEDAKRQFERQGDLLKQGVIAKAEYEPYEIAYKNALEEVEAAENNLE
                     LIKEGQTKKGGQATNTIVRSTAEGMVLDVPVEVGFSVIESNTFNDGTTIASVADMGDM
                     IFEGKIDETEVGKIREGMPLILTIGAIDDASFDAVLEHISPKGIEENGAIQFEIKANV
                     SLKQDQFIRAGYSANAKVVLDRTDSVLAIPESLLLFEGDSAYVEVETAPQMFEKRYIK
                     TGLSDGINIEIKSGLTPEDKIKKPGTSLK"
     gene            complement(3952..5223)
                     /locus_tag="PKM34_00620"
     CDS             complement(3952..5223)
                     /locus_tag="PKM34_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82109.1"
                     /translation="MSIVDYDKWQEIVIVLKKNKLRTFFTAFGVFWGIFLLVVMMGSG
                     NGLKNSALEGFGNMATNSFFMWTQRTTMPYKGFQRGRWYNFKNADAEAVRLNIPEVDK
                     VAPRLQGGGFRGAGSTVVRGERTGAFSTFGDTPEFFEIMPMNVTMGRVINQNDLEEQR
                     KVIVIGDRVYQEMFKKDENPIGEYLQISGVYFKVIGVVKSKQRGPQAEQDEKAIHMPL
                     NTLQQTYNYGDIIGWFSITAKKGVPASVAEQKTKDYLKTRHHIHPDDNRAIGSWNMEN
                     EFNKFSNLFAGITILVWIVGSGTLFAGVVGVSNIMLIVVKERTKEIGIQRAIGAPPSA
                     IMSQIVIESVFLTTLAGYIGLVVGVGLIELVDFMITKSGAESEMFRNPEINFNMAMLS
                     LGILVISGILAGLIPARRAISIKPIDALRDE"
     gene            complement(5358..6599)
                     /locus_tag="PKM34_00625"
     CDS             complement(5358..6599)
                     /locus_tag="PKM34_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82110.1"
                     /translation="MGIFDWDKWQEIFETMKKNRLRTFLTGFSVAWGIFMLIILLGSG
                     NGLENGVKKAFEGDATNTLWINPGQTSTAYKGLKAGRVIQFTNEDYDLISREIPNIYK
                     ISGRVNVWQNNTISYNKEYGNFDIIACHPDYGYYETIDITGGRFINEEDIRDFRKSVV
                     IGKPVKEALFKDEDPIGKYIQVSGVPFKVAGVFSDVGGDRDMQRVYIPLSTAQRVFNL
                     GNRIFNVSVVTKDVTVEESREMAEDVRQTLAQKHKFDPEDQRAVFVWNNLEEYKKLMT
                     LFASIRMFIWIIGIGTIIAGVVGVSNIMMIVVKERTQEIGIRKAMGATPWSIVSLVLM
                     ESILITAFAGYVGLVMGVGLLELISPAFNNSDTFFKNPEVDIFIALGATIVMVVSGML
                     AGFVPARKAAAIQPIEALRYE"
     gene            complement(6675..7385)
                     /locus_tag="PKM34_00630"
     CDS             complement(6675..7385)
                     /locus_tag="PKM34_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82111.1"
                     /translation="MISLRNINKSYGLGKTSLHVLKGIDLDIKKGELVSIMGSSGSGK
                     STLLNVIGILDNHDQGEYILDGLAIRDLTEKKAAQLRNQMIGFVFQSFNLISFKNAME
                     NVALPLYYKGISRKKRNKTAMEMLEKMGLEEWWHHLPNELSGGQKQRIAIARALVSNP
                     RIILADEPTGALDSKTSMEMMDLLKEINQSGITILIVTHEYDIAHSTDRIIHLKDGLI
                     ERTEVNGKNKNQYLDRVN"
     gene            complement(7403..8833)
                     /locus_tag="PKM34_00635"
     CDS             complement(7403..8833)
                     /locus_tag="PKM34_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ82112.1"
                     /translation="MKKLTAAFLILFSLAASSQVKEFTLEDCINFALDSNINIKKQVL
                     MVESQKAQLLQDYLVMLPNLNAGATHGYNWGKTIDRYTNEFATKRVQSNNFYLGTQLN
                     LFQGLRQINTVNQAKLDYAAIQYDLDLMMDDISVAVAGYYLDILYSQELLAVSQEQTG
                     ITRQQVGRMQKMVEAGTIARGDLLNLEAQLATEELQVVEAENRLTIAYLSLQQLIDHP
                     YSPDFRIETPKLKPIQAPQIVLTPQNVYDVALSQRPEIKSAELRVRSAEKGISIARGY
                     LSPTISFSGTLATGYSGLEVPTDETINITGVPIGYLQSNPTEIVVSDYTTSAGNFYIP
                     SFRDQFEDNQNTSLQFSLQIPIFNGWQVRNAISQAKIQKEQADLNLAQAKLDLDKKIQ
                     QAYADAVAALKNFNASEKKVNAQKEAFKYAEQKLAVGLVTAVDYNETKKNLAASESEL
                     LQAKYRFIYTTTILDFYMGKPISLVN"
     gene            complement(9143..11209)
                     /locus_tag="PKM34_00640"
     CDS             complement(9143..11209)
                     /locus_tag="PKM34_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82113.1"
                     /translation="MKNQTGILLFIILLLAGCTTPTTQPANESTEMKLSIVDEASRSD
                     VVRALKEKYGQNQALRIETGVKQAADFWNGTDGTTDEFKAFCMEKFIGNEAELDLVFN
                     RLSAALESLGGCYNKISVDLKLPLHLPLGDIHAVDEILGGYNPGAHFTDDMFENKLAF
                     IVLLNFPFHNLEEKTDKGEGWTRKEWAYARMGELFTSRVPASLNQNISKTLTDADNYI
                     SGYNICMDKLRDNEGRQLFPDNLRLISHWGIRDELKSHFGEEGGLEKQKLIYQVMLRI
                     INQEIPAVVINNNEVTWNPISNEVFQGDAAADNTAEPGTRYEVLLKNFKALSATDAYQ
                     PFYPNQISRAFDEGMEITQEEVEKLFVELVSSPEVKKAGELISKRLGRPLEPFDIWYN
                     GFKAGTSLNEAELNKITRAKYPDNKAFEKDLPNILVKLGFDKDSAAIIASRITVDPAR
                     GSGHAWGAEMRTDRARLRTRIGEQGMDYKGYNIAMHEFGHNVEQTLSLYNIDHYILHG
                     VPNTAFTEALAFLFQRRDLAVLGIAREDPMENHLMALDNLWANYEIMGVSLVDMRVWK
                     WLYEHPDATVPQLKDAVISIAKDVWNSYYAPVFGVKDTPILAIYSHMIDNPLYLSAYP
                     VGHLIQFQVEQYMEGKKLPDEVYRMYTLGLLAPQVWMQQAVGQPVSIRPVLTAAGDAI
                     KALSNR"
     gene            complement(11249..11851)
                     /locus_tag="PKM34_00645"
     CDS             complement(11249..11851)
                     /locus_tag="PKM34_00645"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNQ82114.1"
                     /translation="METIFLTDSPLFTWVILPLLIFLARILDVSIGTLRLIFVSKGFK
                     FYAPILGFFEVIIWLLAIGQIMQHLDNILCYIAYGLGFATGNYIGIYLDEKLSLGTVL
                     IRIVSKSEITGLVDHLRKEEFGASLVDIEGMTGKQKMIFTIVKRIDLKEVMKIIQEHN
                     PHSFVTIEDVKTAREGYFRYSRRRSLVPVVGLFQDFRKGK"
     gene            12193..13701
                     /locus_tag="PKM34_00650"
     CDS             12193..13701
                     /locus_tag="PKM34_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ82115.1"
                     /translation="MQIYYKKKRWKWLLFGAAVIIITASLWYTNVLVRQIAVDERMSI
                     GIWANAIQRKADLVNYTKRFFDQIKEEEYKRALILAEAYRNFRFEESSRTLEFYRQMM
                     ELNTTIPIILTDSKDNIKIYKNVSEKELAGITVMTDSLKKEFSSFEPIKVVLFGEDYD
                     LLYFRESTIFTELRVVLDDLVQSFFSEVVGSSASVPVMITDSTGTRVIESGNIDKEKI
                     KEPEDIAGLIRSMSSENDPIEIDLPGHGKSYIYYMNSTLMEQIMYYPYIMLAIISVFL
                     FISYLLFSTARKSEQNLVWIGMSKETAHQLGTPLSSIMAWLELLKMKGEKSEELSEIE
                     KDVKRLEDITDRFSKIGSPARLEEQNLVDVLYESVAYLEPRISNRVRFHISMEPDRQV
                     IVPLNRQLFEWVVENICKNAVDAMNNAGNINIYIEEVNRHVNIDFEDTGKGMNKSQFK
                     AIFKPGFTSKKRGWGLGLSLSKRIISDYHKGKIFVKSSVVDKGTTIRVVLKK"
BASE COUNT         3230 a         3383 c         3471 g         3780 t
ORIGIN      
        1 attgagggta accggcagat ggtgcttcgg taccatgggt acattttctt tggaatattc
       61 ggccggactg ccatcgggag cagtgtaaac ccggaaaatg ctgaaatcgc cggtatggcg
      121 gggccacatc cagttatcgg tatctccgcc gaatttgcca atggccgaag ggggagcccc
      181 gacgagcctg acatcggtat aaacctcata aacaaacagg tagtactcat tcccgttgaa
      241 aaaactggtg acattgacaa agtatttttt atcgggatta gcggcttttt tcaacctggc
      301 ggttatctcg cgcacttttt cagaacgttt tgaggccgac atggtatcgc tgaggaacgg
      361 aacgattgaa tcggtcacat cttccatcct gacaaagaaa gtagcgctca ggtcttcgtt
      421 cgggagttct tcttccttgc tcatagccca gaaaccgtcg ttgagataat cgtgttcgat
      481 agagctgtgt ttctggatga tatcgtaccc gcagtgatgg ttggtaaata agaggccctg
      541 gcttgacacc acttccgctg tgcagaagaa tccgccgggc tggctgccgt ttccaagccc
      601 gacgatggcg tctttcaggc tggagttatt gatactgtaa atctcttctg cagtgagatg
      661 gagtcccatt ttctgcatat cgacatagtt cagtctctcc acaaaaatgg ggagccacat
      721 cccttcatcc gcccggagtg aaggggaaga cagaaggata aaaagcgaaa aaaagatagt
      781 aaagcgtttc attatcattt tattaagtta tttttaataa tattttacca cgtagtacac
      841 ggagttacac agagggactc ggagtcatta acgattgaaa taaatagacc gaatgactaa
      901 agccccagca tttgttttcc tttttcaaac actacatcca cggggattcc ggaggcattg
      961 atccggtcga ggtctgtttg cagcgccggg gtaacgatcc cttcggtggc cacccagttt
     1021 ttcgcggctt cataatcgcc atcaccctgg atgtagataa ttttctgcac cagtttttcc
     1081 atggccgctt ttgttttgtc gaaatcgacc gtataaatgc cctcttcgct tctggtaaaa
     1141 gcgccttccg accggaagaa gttaaagcgc atcatattgg ccttaccgtg tgcgcttgcc
     1201 gctccaaacc tggaggagcg gaagataccg gcaaagaaag tcacataatt gtccatcact
     1261 tcgccttcgg tcagttcacc catttcatag agtttggtaa cgaggtagag gcccatgata
     1321 tcggctttgc cttcctcgat ggatgaatag gtctctttca gagcggtacg ggccggtcct
     1381 ttgccgttga tggtattttt aaccccaatg gcgtgggcca cttcgtggaa catggtgttc
     1441 tcgaaaaagg cgttgaatgt cacgtgctta cgctgggccg gatcgatcag gagttcggct
     1501 atcggcacca tgatcttatc gaatttggct tgcatcgcat ttttcagttg caatttccgg
     1561 gaacctttct gaagctggac ctcttcgtcg ttgggcaggt tgatggcgat ggttttgctt
     1621 ccggcgttgc aatcgccggc atagtaaaca acgtcatatg cattcaggtc ggcgtcggca
     1681 ccggggattt cggttttgta ctcctctctt accgggagac cacgctgcag gtcgggtagc
     1741 atggcggcat atttgacgag cctggcgctc cagaccgggt ctttaaccag gataaaggat
     1801 tcgaaggccg ctttgtagcc atacaatccg tcttcgtaat tttcgattgg tccgacgacg
     1861 aagtcgagtg ccgagttacg catctccatc caggcaaagt cgctgggctg gtaatcgctg
     1921 gtcagaaggg catcggcacg cagttccagg tattttttca atccggcatc ttcggccagt
     1981 gcggccgctt gtttaatgag ttctgcggct tttttgagct gggtttcata agcctggtgg
     2041 taccacacgc tgcggagtga gccgtcggca ttgcggcgga tcagggtgta gaggcttttc
     2101 ttattgggat cgctgaaggc ttcaaattct tcctttgtca tatcggccgg gtaaaaatta
     2161 gcgccggcag gtttttcgcc gattccgtcg atgaaggaat tattcccgtc gagccggtcc
     2221 caggggccgt aattgatacg ggcaaactgc tttgtggttt cgtcctggat acgtgccagg
     2281 aaaggctctt tcgggcccat ggcctgaagc cagaacagct cgtccatgat gtccgccaca
     2341 tcgaaaaaga tagccacgat ctgcttttcg ttagccgtga gatggctgat gtcggtggta
     2401 agggtaaacg gcgcaaatgt agcgagccgg tttgcaacat gctcaggaga ggccggagca
     2461 agcggttgct gcccggtaac ggtcccggga tactgttgtt ccatctggaa ttcggttttc
     2521 tgcggctgct ggcaggagac ggcaattata accgtaacaa ccagcaggat ttttgaaaat
     2581 acgttattaa gcatgatatt ggttttttag gaaatttgaa cggcgaaggt acttaaaatc
     2641 tgctttcaca gccctttccg tgatgaaaat cgactccaaa cgatgattat ttaagggatg
     2701 ttccgggttt tttgatttta tcctccggag tcagacccga tttaatttca atattgatcc
     2761 cgtcggataa tcccgttttg atatatctct tctcgaacat ttggggagcc gtttccactt
     2821 ctacgtaagc cgagtctcct tcaaagagaa gcaggctttc agggatggcc agcacgctgt
     2881 cggtccggtc gagcaccact tttgcattgg cgctgtagcc tgccctgatg aactggtcct
     2941 gtttcagcga aacattggct ttgatctcga actggatggc cccgttttct tcaatgcctt
     3001 tcggtgaaat gtgttccagc accgcatcga agctggcatc gtcgatagcg ccgatggtca
     3061 gtatcagcgg cattccttcc ctgatcttgc ccacttcggt ttcatcgatc ttaccttcga
     3121 agatcatatc gcccatatcg gcaaccgaag cgatggtggt accgtcgttg aacgtattgc
     3181 tctcgatcac cgaaaaaccc acttccactg gtacatcgag gaccattccc tcggctgtgg
     3241 agcggacgat ggtattggtg gcctggccgc ctttcttggt ctggccttct ttgatcagct
     3301 ccaggttgtt ctctgccgcc tcgacctcct ccagggcatt tttataggca atttcatagg
     3361 gttcgtattc cgctttcgcg atgacgccct gtttcaacaa atcgccctgc cgctcaaact
     3421 gcctttttgc atcttccagc ccgatctttg ccctgttgac gcgcgattgc gcattattca
     3481 gcgtaaccat atcgggaatg atcttgatcc tggcgatcag gtctcctttc cggatcatat
     3541 cgccggcttc gacatagact tcttccacaa ttccggagac ctgcggtttg atcatgatct
     3601 ctttcctggg caccaccgat ccggtagcca cggtactttt tatgatttcc gttttgaagg
     3661 cccgggcagt ttcaaacacc accggcttgg cctgcgattt ctggtaaagg aagtagatcg
     3721 tgtagacaaa gatggatagg acgatcactc ctgccagtat tttcagcgct tttttcatag
     3781 ttggttgttt tgatgagacg caacgtgttg cgtctttact ctgttgttgc tatctgttga
     3841 tgagacgcaa catgttgcgt ctttactgtt gttgctatct gttgatgaga cgcaacatat
     3901 tgcgtcttta ctctgttgct gccatctgtt gagacgcaac atgttgcgtc tttactcatc
     3961 tctgagggcg tcgatgggtt tgatgctgat ggcgcggcgg gcaggtatca ggcctgccag
     4021 tatccctgag atgaccagga ttccgagcga gagcatcgcc atattaaagt tgatctcggg
     4081 gttccggaac atctccgatt ctgcaccgct tttggtgatc atgaagtcga ccagttcgat
     4141 cagcccgacg cccacaacca ggccgatata gcctgccaga gtggttaaaa agaccgattc
     4201 gatgacgatc tggctcatta tcgctgacgg cggtgcgccg attgcccgtt gtatcccgat
     4261 ttccttggtt cgttctttaa cgacgatgag catgatattg ctgacgccga caacccccgc
     4321 aaacaaggtt cccgatccca caatccacac caggatggtg atcccggcaa agaggttact
     4381 gaatttattg aattcatttt ccatattcca ggaaccgatg gcgcggttgt cgtcgggatg
     4441 gatatggtgc cttgttttaa gataatcctt tgtcttctgt tcagccacgg aagccggaac
     4501 cccttttttc gccgtaatgg agaaccagcc gataatatcg ccgtagttgt aggtctgctg
     4561 cagggtattc agcggcatat ggatcgcctt ttcgtcctgc tcggcttgcg ggcctctctg
     4621 tttggatttt accacgccga tcaccttgaa atagactccc gaaatctgca ggtattcccc
     4681 gatcgggttt tcatcctttt taaacatctc ctggtaaacc ctgtcgccaa tcacgatcac
     4741 cttgcgttgt tcctccaggt cattctggtt gatcacccgg cccatggtca cgttcatcgg
     4801 catgatctcg aagaattcgg gtgtatcgcc gaaagtggag aatgcgccgg tcctttctcc
     4861 gcgcacaacc gtgcttcccg ctcccctgaa accgcctccc tgcaatcgtg gcgccacttt
     4921 atcgacttcc gggatattca gccttacagc ctcggcgtcg gcattcttga aattatacca
     4981 ccggccccgc tggaatcctt tatagggcat tgtggtacgc tgggtccaca tgaaaaagct
     5041 gttcgtcgcc atattgccga agccttccag ggccgaattc ttcagtccgt ttcccgaacc
     5101 catcatcacg acaaggagaa aaatccccca gaaaacccca aaggcggtaa aaaaggtcct
     5161 cagtttgttc tttttcagaa caatcacgat ttcctgccat ttgtcgtaat cgacgatact
     5221 catatattga tgttcataat tttcaaaata aggcaggggg ataaaatagc atccctcaga
     5281 gaccccctgc ctatgcaagc attaaccaat tgctgcatta tctttacccg tagccgttgc
     5341 atgcaacggc tccgctgtta ttcatacctg agcgcttcga tcggctggat ggccgcggct
     5401 ttacgggccg gaacaaaacc ggcgagcatg cccgatacca ccattacgat cgtcgctccc
     5461 aaggcgataa aaatatccac ttccggattc ttaaagaacg tatcggaatt attaaatgcg
     5521 ggtgaaatca gctcaagcaa tcccactccc atgaccagac cgacatagcc ggcaaaggcg
     5581 gtaatcagta tcgattccat caaaaccagg ctgacgatgg accatggcgt tgcccccatt
     5641 gctttccgga tgcctatctc ctgcgtccgt tccttcacaa cgatcatcat gatattgctg
     5701 acacccacca cgcctgcaat gatcgtcccg atgccgatga tccagataaa catccggatg
     5761 gaggcaaaca gggtcatcaa ttttttgtat tcttccaggt tattccatac aaacaccgcc
     5821 cgttgatcct ccggatcgaa cttgtgtttt tgcgcgaggg tttgcctgac atcttctgcc
     5881 atttcacggc tctcttcaac cgtgacatcc ttcgtcacca cgcttacgtt gaaaatcctg
     5941 ttccccaggt taaaaacgcg ctgcgctgtg gacaagggga tatagacacg ctgcatatcg
     6001 cggtcgccgc cgacatcgct gaatacaccc gcaaccttga aggggacacc gctgacctgg
     6061 atgtatttac cgattggatc ctcatcctta aaaagagctt cctttacagg tttcccgata
     6121 accaccgact tgcggaaatc cctgatatct tcttcattaa taaatcttcc accggtaata
     6181 tcaatggttt cataataacc ataatcggga tggcaggcaa tgatatcgaa attcccgtat
     6241 tctttattgt aagaaatggt gttgttctgc caaacattca cccggcctga aatcttgtaa
     6301 atattgggga tttcgcggct gatcaggtca taatcctcat tggtgaactg aatgactctc
     6361 ccggctttca gtcctttgta agccgtgctg gtctgcccgg ggttgatcca gagggtattg
     6421 gtcgcatcgc cttcaaacgc ttttttgacg ccattttcga gtccattgcc tgagcccagc
     6481 aggatgatga gcataaagat gccccaggcc acgctgaatc ccgtcaggaa ggtgcgcaac
     6541 ctgtttttct tcatggtctc gaagatctcc tgccatttat cccagtcaaa gattcccatt
     6601 gttgccggtt atgtttagac gttgagagac gttgcatgca acctagacgt tgcaagcaac
     6661 gtctttactt aatattagtt gactctatca agatactgat ttttattttt tccattgact
     6721 tctgttcgct cgatcaggcc atccttcagg tggatgatcc ggtcggtcga atgggcgata
     6781 tcgtattcgt gcgtcacaat caggatcgtg atgccgctct ggttgatctc cttgagcaga
     6841 tccatcatct ccatggatgt tttggagtcg agcgcccccg taggctcatc ggcgaggatg
     6901 atccgggggt ttgaaaccag cgccctggct atggcaatgc gctgtttctg accgcccgac
     6961 agttcattcg gcagatggtg ccaccattct tccagtccca tcttttccag catttccatg
     7021 gcggttttgt tcctcttttt ccggctgatc cccttatagt aaagaggtaa tgccacgttt
     7081 tccatggcgt ttttgaaaga gatcaggtta aacgactgga aaacgaaacc gatcatctgg
     7141 ttgcgtaact gggctgcttt tttctccgtc aggtcccgga tcgcaaggcc atccaggata
     7201 tattccccct gatcgtggtt gtcgaggatg ccgataacat ttaaaagggt agattttccg
     7261 gagcctgacg atcccatgat cgaaaccagc tctccctttt taatgtcaag gtcaattcct
     7321 ttcagcacgt gcagggaggt tttgcccagc ccgtatgatt tgttgatgtt gcgcaagctg
     7381 atgattgtat cttccatcat ggttaattta caagggaaat gggttttccc atatagaaat
     7441 ccaaaatagt agtcgtatag atgaagcggt attttgcctg caggagttcg gactcggaag
     7501 ccgccaggtt cttctttgtt tcattataat cgacggctgt tacaagcccg acagccagtt
     7561 tctgttcggc atatttgaat gcctctttct gtgcattgac ttttttctct gaagcattga
     7621 aatttttcaa agcggcaacg gcatcggcat aggcctgttg aatcttcttg tcgagatcga
     7681 gtttagcctg ggccaggttg agatcggcct gttctttctg gatctttgcc tggctgatag
     7741 cattgcgcac ctgccagccg ttgaaaatgg gtatctgcaa tgagaattgt aggctggtat
     7801 tctggttatc ttcaaactgg tctctgaaag agggaatata gaaattgcct gcggatgtag
     7861 tgtagtcgga aaccacaatt tctgttggat tgctttgcag gtaaccgatc ggaacgccgg
     7921 ttatattaat ggtctcatcg gtaggcacct ccaggccgga ataaccggta gccagggttc
     7981 cggagaaaga gatcgtggga ctgaggtatc cgcgggcaat ggagattcct ttttcagcac
     8041 tcctgacgcg cagttcggcg ctttttatct cggggcgctg ggagagggct acatcgtaaa
     8101 cgttttgcgg tgtcagcaca atttgaggcg cctggatcgg tttcagcttc ggggtttcga
     8161 ttcggaaatc gggtgaatac ggatgatcga taagctgctg caggctcagg taggcgatgg
     8221 tcagcctgtt ttcggcctcg accacctgga gttcttcggt agccagctgg gcttccaggt
     8281 tgagcagatc accccgggct atcgtccccg cttcgaccat tttctgcatc cggccgacct
     8341 gctgccgggt gatcccggtt tgttcctgcg aaacggccag cagttcctgg ctgtagagaa
     8401 tgtcaagata gtatccggct acagccactg agatatcgtc catcatcagg tcaagatcat
     8461 actgaattgc ggcataatcg agtttggcct ggttcacggt gttgatctgg cgcaatccct
     8521 ggaaaaggtt cagttgggtt cccaggtaaa aattattcga ctgaacgcgt tttgtggcga
     8581 attcgttggt gtacctgtca atcgtctttc cccagttgta accgtgggtg gcgccggcat
     8641 tgaggttggg aagcatgacc aggtagtcct ggagcagttg ggccttctgg ctttcgacca
     8701 tcaacacctg ctttttaatg ttgatgttgc tgtcgagggc gaaattgatg caatcttcca
     8761 gtgtaaactc tttcacctgg gaggatgcgg ccagggaaaa aagaatcagg aatgcggcgg
     8821 tcaatttttt cacaatgttt ttaagaacgc ttttttggtt aatgcttttg gtgtacatag
     8881 ttaatctgag ttagattatt tttttaagta acgcgacttt gcagcgaaaa atatgccagt
     8941 atcataattt attgatattt aatattataa aatgttgtat gatgatatcg ttaaaaaagt
     9001 cgtgttcgcc agctaacaac acttgttcga taacgaacaa ttaatttgca aatatggcag
     9061 gagaaatcaa aagaattcgc gcctgatttg caacagggcg ggaagacttt acgaatggca
     9121 gcaattggga attcagtgtc ttttaccggt tcgagagtgc tttgatggcg tcaccggcgg
     9181 ccgtcaggac cggccggatc gaaacaggct gtccgaccgc ctgttgcatc cacacctgcg
     9241 gcgccagtaa gcccagtgtg tacatccggt agacttcatc gggcagtttt ttgccttcca
     9301 tgtactgttc cacctggaac tggatcaggt ggcccacggg ataagccgac aggtagaggg
     9361 ggttatcgat catatgcgaa taaatagcca ggatgggtgt gtccttaacc ccgaaaaccg
     9421 gtgcataata gctattccag acatcctttg caatggagat aacggcatct ttcagttgcg
     9481 ggacggtcgc atccgggtgt tcgtacaacc atttccagac gcgcatatcg accagggata
     9541 cgcccatgat ctcataattg gcccacaggt tgtcgagtgc catcaggtgg ttctccatcg
     9601 gatcttcccg ggcaatcccc agcacggcca ggtcgcgccg ctggaacagg aaagccagtg
     9661 cctcggtaaa agccgtgttg ggcacgccgt gaagaatgta atgatcgata ttgtagagcg
     9721 aaagggtttg ctcgacatta tgcccgaact cgtgcatggc gatattgtag cctttgtagt
     9781 ccattccctg ctccccgatc cgggtacgga gccgggcgcg gtcggtccgc atttcggcgc
     9841 cccaggcatg gccggagcca cgtgccggat ccaccgttat ccgcgaagcg atgatcgcgg
     9901 ccgaatcttt gtcaaatccc agttttacca ggatattcgg cagatctttc tcaaaggcct
     9961 tattatccgg atacttagcg cgggtgatct tattcagctc cgcttcgttg agagaggtgc
    10021 ctgctttgaa accgttgtac cagatatcga acggttccag tggccgcccg agcctcttgc
    10081 tgataagctc tccggccttt ttaacttccg gagaggagac cagttcgacg aaaagttttt
    10141 cgacctcttc ctgtgtgatc tccatccctt cgtcgaaggc gcggctgatc tggttgggat
    10201 aaaaaggctg gtaggcatcg gtggccgata aggctttgaa gtttttcagc aacacttcgt
    10261 agcgcgttcc cggctcggct gtgttgtcag cagcggcatc tccctgaaag acctcattgg
    10321 agatcggatt ccaggtcact tcgttgttgt tgatgacaac tgccgggatc tcctggttga
    10381 tgatgcgaag cataacctgg tagatcaact tttgcttttc cagtccacct tcctctccaa
    10441 agtgtgattt cagttcgtcg cggatgcccc agtgggagat cagccggaga ttatcgggga
    10501 aaagctgccg cccttcatta tccctgagct tgtccataca gatgttgtac ccggagatgt
    10561 aattatcggc gtcggtcagg gttttgctga tattctggtt caaagaagcc ggcacccggg
    10621 aggtgaacag ttcacccatc ctagcatagg cccattcttt ccgcgtccag ccctcccctt
    10681 tatcggtctt ttcttccagg ttatggaacg gaaaattgag caggacgata aaggccagtt
    10741 tgttctcgaa catatcgtcg gtaaaatggg ctcccggatt gtaacctccc aggatttcat
    10801 ccaccgcgtg gatatcgccg agtggcaggt gcagcgggag cttcaggtcg acgctgatct
    10861 tgttgtaaca accgccgagg gattccagtg cagccgacaa acggttaaaa acaaggtcga
    10921 gttccgcttc attaccgata aatttctcca tgcagaacgc tttaaactca tcggttgtgc
    10981 cgtcggtgcc attccagaag tcggccgcct gtttcacacc ggtttcgatg cgtaaagcct
    11041 ggttttggcc atatttttct tttaatgcgc ggacgacatc gctccgggac gcttcatcga
    11101 caatactcag tttcatttcg gtgctttcgt tagcgggttg tgttgttggc gtggtgcatc
    11161 ccgccagcag aaggataata aataagagga ttccggtctg gtttttcatc gtgtctgaat
    11221 tatatgtttc agccacgaaa ttaaggaatt atttcccttt acggaaatcc tggaataagc
    11281 caacgacggg gacgaggctt ctccgccggg aataccggaa atacccttcc ctggcggttt
    11341 ttacatcctc gatcgtcaca aatgagtgtg gattgtgttc ctggatgatc ttcatcacct
    11401 ctttcaggtc aattcgcttt acgatcgtaa atatcatttt ttgtttaccg gtcatccctt
    11461 caatatcgac gagtgaagcg ccgaattcct cttttcgcag gtgatcgacc aggccggtga
    11521 tttcggattt ggagacgatg cggataagga cggtccccag cgaaagtttt tcatccagat
    11581 atataccgat gtaattgccg gtagcaaaac cgagcccgta ggcgatatag cacaaaatat
    11641 tatccaggtg ctgcatgatc tgcccgatgg ccagcaacca gatgatcact tcgaaaaatc
    11701 ccaggatcgg ggcatagaac ttgaatcctt tagagacaaa tatcaggcgg agcgtaccga
    11761 tgctcacatc caggatgcgg gccagaaaga tcagcaaggg gaggataacc caggtgaata
    11821 gcggtgaatc ggtcagaaat attgtttcca taataatatg aaggttatat tttcttgaat
    11881 ctgtttcata agataagcac ttcctttatt ttgtttgtag tccgacgact gaagccgtcg
    11941 gttccctctt tcgtaaaacc ataggaacca acgtctttag ttgttggagc acgaactaat
    12001 gtcaggccat tattgtataa tatcagtctt ttgagacaac cacaattata ccagaggttt
    12061 tcaatgtcat ttggagcaac ctcttccggg tattatcttt aatcttttta taaatttgtt
    12121 gaaataatct ggttttcata aggttttcaa taaaatccga aaaaatacgt taggcagaaa
    12181 agagtttacc cggtgcaaat ttactacaag aaaaaaagat ggaagtggtt attgttcggt
    12241 gcggctgtta tcattatcac ggcatcgctc tggtacacca acgtgctggt gcggcagatc
    12301 gcggttgatg agcggatgag catcggcatc tgggctaacg caattcagcg taaagccgac
    12361 ctggtcaatt ataccaaacg gttcttcgac cagatcaagg aagaggaata caagcgggct
    12421 ttgatcctgg ctgaagccta ccgtaatttc cgattcgaag agtcgtcgcg gacactggag
    12481 ttctaccggc agatgatgga actcaacacg acgatcccca tcatacttac tgattcaaaa
    12541 gataacatca agatatataa aaacgtcagc gaaaaagaac tggccggcat taccgtaatg
    12601 accgacagcc tgaaaaagga gttttcctcc tttgagccga taaaagtagt cttgttcggc
    12661 gaagattatg atcttttgta tttcagggaa tcgacgatat ttactgagtt gcgggtggtg
    12721 ctcgacgacc tggtgcaatc gttcttctcg gaagtggtgg gaagctctgc ttcggttccg
    12781 gtcatgatca ccgacagtac gggtacccgg gtaattgagt cgggaaatat cgacaaagag
    12841 aagatcaaag aaccggaaga cattgccggc ctgataagaa gcatgtcatc ggaaaacgac
    12901 ccgatagaga tcgatcttcc cggtcacgga aagagttata tctactacat gaactccaca
    12961 ctgatggagc agatcatgta ttatccctac atcatgctgg cgattatcag tgttttcctg
    13021 ttcatctcct acctgctgtt cagtacggcc cggaagtcgg aacagaacct ggtatggatc
    13081 gggatgtcga aagagacggc acaccagctc ggtacgccgt tatcatcgat catggcgtgg
    13141 ctggagttgt tgaagatgaa gggggaaaaa tcggaggagc tgtcggagat cgagaaagat
    13201 gtcaaacggc tcgaagatat caccgaccgt ttctccaaga tcggttcgcc ggccaggctg
    13261 gaagaacaga acctggtgga tgtcctgtac gaatcggtgg cttacctgga gccccgaatc
    13321 tcgaacaggg tacggttcca tatcagcatg gaacccgacc ggcaagtcat tgtcccgctc
    13381 aaccggcagc ttttcgaatg ggtcgtagaa aatatctgca aaaacgcggt ggacgcgatg
    13441 aacaacgccg gcaacatcaa tatttacatc gaggaggtta accgccatgt caatatcgat
    13501 tttgaagata ccggcaaagg gatgaacaag tcgcagttca aggctatttt caaacccggt
    13561 tttaccagca aaaaaagggg ctggggcctc gggctttccc tttccaaaag gatcatttcc
    13621 gattatcata aaggaaagat cttcgtgaag tcgtcggtgg tggataaggg cacgacgatt
    13681 cgggtggttt taaagaagta aaagggaccg tctcaaaagt acaaatattc gcttttaatt
    13741 ggtagtccga cgactgaagc cgtcggttcc acctcgtaaa atcataggaa ccaacggctt
    13801 tagtcgttgg agcacgaact gatctcaggc cattatagta taatatcaga cttttgagac
    13861 agcc
//
LOCUS       DAOQXS010000010        13822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_4913_length_13822_cov_3.842086, whole genome shotgun sequence.
ACCESSION   DAOQXS010000010 DAOQXS010000000
VERSION     DAOQXS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4913_length_13822_cov_3.842086"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2827)
                     /locus_tag="PKM34_00655"
     CDS             complement(<1..2827)
                     /locus_tag="PKM34_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ82116.1"
                     /translation="MRAIKVFAALVFLILAASLQGQNGTIRGTVYDDATGEYLPGVTI
                     FLEGTTLGTLTDLDGKFNLSAPAGNYDLRVSFISYEAMIIKGIRVEEGDVTLFDNLRL
                     KEATVELTEVVIAAQGIRNTENALLTLKRKSANVIDGISATGFKKIGDSDAAASMKRV
                     TGVSVEGGKYVYVRGLGDRYTKTILNGVDIPGLDPDRNTIQMDIFPTNIIDNIIVHKS
                     FSADLPADFTGGVIDIAIKDFPEERKGSIAFGLAYNPGFHFRSDYLTYEGGKTDWLGY
                     DDGTRAIPATENIPQFVEAYLDPNGKAGLRYKEILGGFNTTLAAREQTSLMDYNIGAT
                     YGNQHVGNIVTLGYNISLSYKSNTEMYKNAEYGRYGLTDSNTFEMDVREYQKGNFGVS
                     SVLWSGMAGFAVKTKASKYRINIVHLQNGESRAAVFEYQGSDQGSIFGGFQHNLEYSQ
                     RSLTNLIISGKHNLAGSEWEIEWKLSPTLSKMYDPDIRFTRYKADNGKFIIGTETGFP
                     ERIWRDLEEISYAGIVSVTRQFDLWKRNARVMFGGAYTSKERDFIIRNYAINIRNVPL
                     TGDPDEIFSQENLWPYNDNLNYGTSYEAAFYPNNANRFNSGVRNMAGYVSAEVSPFNR
                     LKAIIGVRVENYVQRYTGQNQQGTIVPDNEKVLDNTGFFPALNLIYALNEQQNLRFSY
                     ARTIARPSFKELSYAEIYDPISGRTFIGGLFQDADEQLGIEYWDGNLVSTDIDNIDLR
                     WELYKPNGQMVSVSAFCKMFDNPIEIVQYAVQAGSFQPRNVGDGQVFGAELEFRFNLI
                     NISQELRNLSLSSNLTYTRSFIRMSNVEFQSRVDNARVGETVGEYRDMAGQAPYIINL
                     GLSYDGGEKGFWNGFEAGLFYNVQGKTLQYVGMVDRPDIYALPFNSLNFNSNKSFGKN
                     NKFQVGIKAENILADWKDAVFISYMATD"
     gene            complement(3017..4459)
                     /locus_tag="PKM34_00660"
     CDS             complement(3017..4459)
                     /locus_tag="PKM34_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82117.1"
                     /translation="MKKILFYFVIFSSLIAFLTSCDKDENNMAPAVTAPANANVEINA
                     AIDLTFNFTAEAGFKSSAVTATNGTASVKTDGTAGSTSGSVVVTFTAGANAGAGSVVL
                     TLTDNDDQAGSATAVLNVVEEELIISVPGNITENTTWETGKVYILESRIAVVSGATLT
                     IEPGVIVKGQAGTGANATALLIARGGKIMAEGTADAPIIFTSVADEIEPGMIESPNLD
                     PVLNGLWGGLLILGNAPISADAASMQIEGIPPSDQNGLYGGDNAADNSGVLRYISIRH
                     GGANIGEGNEINGLTLGGVGSGTVIENIEIVSNQDDGIEWFGGTVSVKNAIIWNTGDD
                     AVDTDQAWAGTLDNFIVVNPGDECMELDGPEGALDNAGHTIKNGTVYAGNAEGLVDLD
                     ENTRCTITGIYFFGVAEGQDFDLNPAVLTCSNLQATLPGGAVVTDYFKGGSDAYVTIV
                     AEGANTVGADVTKFQTWSWAAVSGALNGFQ"
     gene            4754..5527
                     /locus_tag="PKM34_00665"
     CDS             4754..5527
                     /locus_tag="PKM34_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ82118.1"
                     /translation="MSNEPKSIHEFDFNLICEYFSSVERQGPGSPEMTIKALSFIDNL
                     TADSKIADLGCGTGGQTMVMARNVPGKITGIDLFQKFIDLFNQNAGNLNLQHRVKGIV
                     GSMDNLPFQPEELDLIWSEGAIYNIGFERGINEWRKFLKPGGFLAVSEASWFTPERPA
                     EIEEFWKDAYPEIDTIPNKVLQMQNAGYVPVATIVLPENCWIENFYAPLVKAREIFLQ
                     KYAGNKTAEEFIASELHETQLYFKYKAFYGYVFYIGKKI"
     gene            complement(5706..6656)
                     /locus_tag="PKM34_00670"
     CDS             complement(5706..6656)
                     /locus_tag="PKM34_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82119.1"
                     /translation="MKVLTSLIFLLFLLPGSIYGQGSSKAAYIISNDADTLFGAGNMG
                     PEQDFCLFREIGSDDARVYNPDEISAFRYINGKYYVSKQIKNKSGEVKWCFLEFLVDG
                     EIDLFRIRNAHRYFIKRDNQDLLELDDRNTEVVNSDGRYYLKKNRTFNGLMRYYMSDA
                     PAIFPEIDKIEDITQKSMVKIAIDYHNIVCKDAECINYTKSISKFIVSWKLEAVSGAT
                     YHNAYYSPYYGLQVHLGRKENIEHMFLKTGLIYSDKIYWAKDNTSSGERQYKIRIPLS
                     FQYVFCKGVVRPTVAIGLALRLFNQPVGPGRSVVVHFKAI"
     gene            complement(6948..8141)
                     /gene="kbl"
                     /locus_tag="PKM34_00675"
     CDS             complement(6948..8141)
                     /gene="kbl"
                     /locus_tag="PKM34_00675"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNQ82120.1"
                     /translation="MYGKLQDWLKNELKSIEEAGLFKKERIIVTPQGAEIKVSGGQEV
                     LNFCANNYLGLSNHPKLVKAAKETLDSHGFGMSSVRFICGTQDIHKILEKKIAEFFGT
                     EDTILYAACFDANGGVFEPLFGEEDAIISDSLNHASIIDGVRLCKAQRFRYNNADMAD
                     LEEKLIEASKTARFKIIVTDGVFSMDGNVAPMDKINELAKKYDALIMVDECHSAGVVG
                     KTGRGVTELFNIRGEVDIITGTLGKAFGGGIGGFTTGRREIIELLRQRSRPYLFSNSL
                     IPAAVGAGIAMFDMMAETNELQDKLHANTDYFLEKMKAAGFDIKPTKSAICAVMLYDA
                     KLSQVMAEKLLAEGIYVIGFYYPVVAKGLARIRVQISAAHERHHLDKCIDAFTKVGKE
                     LGVIK"
     gene            8399..9391
                     /locus_tag="PKM34_00680"
     CDS             8399..9391
                     /locus_tag="PKM34_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNQ82121.1"
                     /translation="MKKLLKIFLWFVLVIVIIIVIAVGYIQLTWNKTFDAPYPDITAS
                     NDPAMIERGKYLAYGWSHCAACHVPADKVREVENGLEMPLIGGWDEEIAGFGTFRAPN
                     LTPDPETGIGRMTDGELARAIRYCVKHDGKLLPPFMVFQGLSDEDLTAVISFLRSQEP
                     VKNQVAPSDYGFVAKAVIAFGLLKPQGPKSTPPASIRPDTTAVYGEYLATSVGNCLGC
                     HIKIDQAGNQVNPDFAGGGIFPPTAFSKGYGFVSPNLTPDKKTGAIADWSEEQFVNRF
                     QGGRLHEGSPMPWNMYSRMSVNDMKAIYRFLHTLDPVGFSVEKTVYQPGEKMPQ"
     gene            complement(9385..10254)
                     /locus_tag="PKM34_00685"
     CDS             complement(9385..10254)
                     /locus_tag="PKM34_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ82122.1"
                     /translation="MIRKYLLQQYPYEENKWKIILPISIFILFFMPVFQPFGLSEFQS
                     PDKLLILAGYGLVTFIILMIDMILIPVVFPKVYSEEKWTVFKEMLNFLLILFTIGLGN
                     LVYSHFYIGFRLTFQNILIFQLFTVAIGIIPVIAIVMIKQSYLNRKNAVSAGMITSGL
                     HPRPDVETQFSALKIFSEGGKEELEILFDDLVFIKSEGNYISTGYIKKGKMVRTLIRN
                     TMKYAEDLIAPIPAAYKCHRSWIVNLDKIEKVSGNSQGLKLTLAGFEEPVPVARNLAS
                     DFKRVMGERQATY"
     gene            10313..11008
                     /locus_tag="PKM34_00690"
     CDS             10313..11008
                     /locus_tag="PKM34_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82123.1"
                     /translation="MLLIRLSGHSCAGKTRLVNALPKYGITLPKVLRYTSRAPRPEEI
                     EGEDYFFRSREYIEALPGKDFLKGPVRNMLHAFDLNAIANLLGNHTGVIIEIYPGLWP
                     ELVSRLRIRLGNNLKTASVFMTAVDPERIRQFKKKDEIAGFISSEVYKMLLFRNKDEI
                     EDIRIRAYSAALEITGALSPQGRKEYDRIIFSAPEGPDGEDQWTRGPQPVGQALMALN
                     KFREFLYEFEKPY"
     gene            complement(11013..11693)
                     /locus_tag="PKM34_00695"
     CDS             complement(11013..11693)
                     /locus_tag="PKM34_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ82124.1"
                     /translation="MIEIKNLNKYYGDHHVVKNARMVFEKGCVYGLVGENGAGKTTLF
                     KCIAGLEKHDGEIITDYGHLKDHLGYLPTEPYFFQKITGMEYIRLLCNARRIKAENIH
                     EKNIFDLPLNQYASTYSTGMKKKLALLAILMQENEVFILDEPFNGVDIQSNILIADII
                     LRMKELGKTVIISSHIFSTLSETCDEIFLMKEGAIIRRVLRADFPALEHEMKQILVGD
                     RIEKLGLR"
     gene            complement(11690..12607)
                     /locus_tag="PKM34_00700"
     CDS             complement(11690..12607)
                     /locus_tag="PKM34_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82125.1"
                     /translation="MKLYFLIQFKMLNRHLAAFGIHPVIGCVLILAAFTGSSVLIFEK
                     TAFAEYLYLAASLSFLARLSDPARNQFLRSCFGKKDYRVVRMLENVIVAAPFAAFLVI
                     EKYYFHALAALFLAGFMSLTIVGNRSGFTIPTPFGKRPFEFTVGFRNSFPVIVLAWFL
                     TVMAVISGNFNLGIASLILVFLVCFSYYLNPENEFYVWIFNSRPGIFLFGKIKTALVY
                     ATFLTLPVSLVLSVFYSDKILIIGAFQLLGYIYLGAVVLAKYSSYPHPMNLPQFIILV
                     VSVWFPPLLLAVIPFFYRKSSIRLKTLLA"
     gene            complement(12604..>13822)
                     /locus_tag="PKM34_00705"
     CDS             complement(12604..>13822)
                     /locus_tag="PKM34_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ82126.1"
                     /translation="HPSNSAIKADVLVVAQHGIGPLMRVLEVIESEGFKGFKGGKGGK
                     GFEDWGIGGLSNVPNLCLPGEDGFVFTGMVEEKVDYDEDFTRWDLISEMPEKIPLRTS
                     IGCPYRCRFCDFCSLFPKIFLRSPESLCKELSMVKSRTGSTPAMIHVTDDNVFITRKR
                     LFEICGTLSKSGLNHWAGFMRGGEYSEDEMKAILDSGLMMGKMGVESGDQGQLDRMNK
                     KQKIDRVKRGIEQFDAQGIGVLMTFVVGFPGETEETLRNTIDFMNNLALTHLGVSYQV
                     YPLLIFNLSELAEPAIRKKWKIEGFMDQWSHYSMNSEQADNACREVFRSVTNVPYNYS
                     EESNFFNRGMFDPDTRRKLFHLRQQLTVHLLDRENMMDNEHIIKEMARLMELPAEETG
                     MVFTDEIVIPGSK"
BASE COUNT         3607 a         3452 c         3158 g         3605 t
ORIGIN      
        1 gatcggtagc catgtaagaa atgaaaaccg catctttcca atccgccagt atattttccg
       61 ctttaatccc cacctggaat ttattgtttt tcccaaagct cttgttgctg ttgaaattca
      121 ggctattaaa gggaagggca tagatgtcgg gacggtcaac catgccgaca tactgaaggg
      181 ttttcccctg gacattatag aatagtccgg cttcgaaacc gttccagaac cctttttccc
      241 caccgtcata cgacaatccc aggttaatga tatatggcgc ctgtccggcc atatcgcggt
      301 actccccgac cgtttcacca acccgcgcat tatccaccct cgattgaaat tcgacattgc
      361 tcatccggat aaatgaccgg gtataggtca ggtttgagga taaagagaga ttcctgagct
      421 cctggctgat atttatcagg ttgaaccgga attcgagctc ggcgccgaaa acctgcccgt
      481 cgcccacgtt tcgcggctgg aaagagccgg cctggacggc gtattgcacg atctcgatcg
      541 ggttgtcgaa catcttgcag aaagcgctga ccgacaccat ctgcccgtta ggtttgtata
      601 gttcccagcg aagatcaata ttgtcgatgt ctgtcgaaac caggtttcca tcccagtatt
      661 cgatccccag ttgctcgtcg gcatcctgga aaagtccgcc gataaaagtc cggccgctga
      721 tcgggtcgta gatctcggca tacgataact ctttgaagga aggacgggcg atggtccggg
      781 catagctgaa gcgcaggttt tgctgctcgt tcaaagcata gatgagattc agggcaggga
      841 aaaagccagt gttatccaga actttttcat tatccggcac gatggtgccc tgctggttct
      901 ggccggtgta ccgctgaaca tagttttcaa cccttacgcc gataatggct ttgagccggt
      961 taaaagggct gacctccgct gaaacataac cggccatgtt gcgaacaccc gaattaaacc
     1021 ggttggcatt attcggatag aaagccgctt cataacttgt tccgtaattc aggttatcat
     1081 tatacggcca taggttctcc tggctgaaaa tttcatcggg atcgccggtc agcggcacat
     1141 tccggatatt gatcgcataa ttcctgatga taaaatcacg ctccttggag gtgtacgctc
     1201 cgccgaacat gacccgggca tttcttttcc acaggtcgaa ctgccgggtc acgctgacaa
     1261 tccccgcata gctgatctcc tccaggtcgc gccatatcct ctccgggaaa ccggtttcgg
     1321 tgccgatgat aaatttaccg ttgtcagctt tataccgtgt aaaccggata tcgggatcat
     1381 acattttgga taatgtcggc gacagtttcc attctatctc ccattctgat ccggccaggt
     1441 tatgtttccc gctgatgata aggttggtca gcgagcgctg gctgtattcc aggttgtgct
     1501 ggaaaccccc gaagatactc ccctggtcgg agccctggta ttcaaaaaca gcggccctgg
     1561 attcgccgtt ctggaggtgc acgatgttga tcctgtattt cgaagccttc gttttaacgg
     1621 caaatccggc catgccgctc cacaaaacac tgcttactcc gaaattacct ttctggtatt
     1681 cacgcacatc catctcaaag gtgttggaat cggtcaggcc ataacggccg tattcggcgt
     1741 ttttatacat ctccgtattg cttttatagg aaagcgagat gttatatccc agggtgacaa
     1801 tatttcccac atgctgattt ccatatgtag cgccgatatt gtaatccata agactggtct
     1861 gttccctggc agccagggta gtattaaaac cgcctaaaat ctctttgtac cgcaatccgg
     1921 ctttcccgtt aggatcaagg taagcctcca caaattgcgg aatattttcg gtggccggaa
     1981 tagcacgggt gccgtcgtca tacccgagcc agtcggtttt tccgccttca taggtcagat
     2041 aatcgctccg gaaatgaaag ccgggattat aagccaaccc aaaggcaata ctgccttttc
     2101 gttcctcagg aaagtcttta atggcaatgt cgatcacgcc gccggtaaaa tcggccggca
     2161 gatcggctga aaaggatttg tgaacgatga tgttatcaat gatattggta ggaaaaatgt
     2221 ccatctggat ggtattccgg tcgggatcca gcccgggaat atcgacaccg ttcaggattg
     2281 ttttcgtata cctgtcgccc aggccgcgga catacacata tttccctcct tcaaccgata
     2341 ccccggtgac cctcttcatg gaagccgctg catccgaatc cccgatcttt ttaaatcctg
     2401 tggctgaaat accatcgatc acattagccg attttcgctt cagggtgagc aaggcatttt
     2461 cggtatttct gatcccctgc gccgcaatca ccacttcggt caattccacg gtcgcctctt
     2521 tcagccgtaa gttgtcgaat aacgttacat cgccttcctc tacccggata cctttgatga
     2581 tcatggcttc ataggatatg aacgaaaccc tcaggtcata atttcccgca ggggcgctca
     2641 ggttgaattt cccgtcgaga tcggtgaggg tcccgagggt agtcccttcc agaaaaatag
     2701 taacaccggg caggtattcc ccggtggcat catcataaac ggttccacgg atcgtcccgt
     2761 tttggccctg taaagaggcg gccagaatca ggaaaaccaa cgctgcgaat acttttattg
     2821 cccgcatagt ttgtgttcta atcattatta agtaacagga tattaaataa acaatcttcg
     2881 tggccctccg tgccttcttc atgtttctca gcgtaactta tgagtatacg ttacacgaag
     2941 attcacgaag gagtcacgca ggatcacgaa gtttaaaaca aattgattaa agggttaagc
     3001 ccactttatt cttaagtcat tgaaatccat tgagcgctcc tgaaacggcc gcccatgacc
     3061 atgtctggaa tttggtaacg tcggcgccaa cggtattggc tccttcagcc actatggtta
     3121 cgtaagcgtc gctgccccct ttgaaataat cggtgaccac agcacctcca ggcagtgtag
     3181 cctgaagatt ggaacaggtc agtactgccg ggttcaggtc aaaatcctgg ccttcggcta
     3241 caccgaaaaa gtaaataccg gtaatggtgc aacgggtatt ttcatccagg tccaccaatc
     3301 cttcggcgtt tccggcataa acggttccgt ttttaatggt atggccggca ttgtcaagcg
     3361 caccttccgg gccgtcaagt tccatacact cgtctcccgg gttcacgacg ataaaattat
     3421 cgagcgtgcc ggcccaggcc tggtcggtat caaccgcgtc gtcgcccgta ttccagatga
     3481 tcgcgttttt aacggaaacg gttccgccga accactcgat gccgtcatcc tggtttgata
     3541 cgatctcgat gttttcgata acggttccgg agccaacgcc tcccagggta aggccgttga
     3601 tttcgttgcc ttcgccgatg ttggcgccac cgtggcggat ggagatgtaa cgcagtacac
     3661 ccgaattgtc ggcagcattg tcgccaccgt aaaggccgtt ctgatccgac ggcggaatac
     3721 cctcgatctg catggatgcg gcatcagcgg agataggcgc atttccgagg atcagcaaac
     3781 caccccacaa accgttgaga accggatcca ggttcgggct ttcgatcata cccggttcga
     3841 tttcatcggc cacggaggtg aaaataatgg gtgcatcggc agtgccctcg gccatgatct
     3901 tgcccccgcg ggcaatcagg agagcggtgg cgttagcgcc ggtacccgcc tggcctttaa
     3961 cgataacacc cggctcaatg gtcagggtag cccccgaaac cacggcaata cggctttcca
     4021 ggatatatac tttacccgtt tcccaggtgg tgttttcagt gatgtttccg ggaacggaga
     4081 taatcagttc ttcttcaacg acgtttaaaa ccgccgtcgc tgaaccggcc tggtcgtcat
     4141 tatcggttaa ggtaagtacc acagaacctg cgcctgcatt ggcgccggcg gtgaatgtca
     4201 ccacaacgga gcctgatgtg cttccggcag taccgtcggt ttttaccgaa gccgtaccgt
     4261 tggttgctgt cacagcagag gatttaaacc cggcttcggc cgtaaaatta aaggtcaggt
     4321 caatagctgc attgatttca acgtttgcat tggcaggggc agtaacagcc ggtgccatgt
     4381 tgttttcgtc tttatcgcag gaagttaaga atgcgattaa cgatgagaag atcacaaaat
     4441 agaagagaat ttttttcata tcagtatcgg ttaaattttt tgcaaaagtc acgataccag
     4501 attaacactt tgttttacaa ttgttaagaa attgttatgg gtgatgaggt tgtattaagg
     4561 aaagattaat aatatatcat tcgcatcaaa aattccatac ctttgccggg actaaaacca
     4621 ttggatgtta ttctgtaaat cggattcggt gaggtaaata atcttcggtg ttctgccgga
     4681 gattatatct tccattcaaa cgggcatttt catgccctct gttctattta tccattttaa
     4741 aacattttac atcatgagta acgaaccgaa atccatacac gaattcgatt ttaatttaat
     4801 ctgcgaatat ttttcaagcg ttgaacgaca gggccccgga agtcctgaaa tgaccattaa
     4861 agcgctgagt ttcatcgata acctcaccgc tgacagcaag atagccgacc tcggttgcgg
     4921 aaccggcgga caaaccatgg taatggcccg gaatgttccc ggaaagatca ccggaatcga
     4981 cctttttcaa aagtttatcg acctgtttaa tcaaaatgca ggcaacctga atcttcagca
     5041 cagggttaaa ggcatcgtgg gatcgatgga caacctgccc tttcagcctg aagaactgga
     5101 cctgatctgg tcggaaggcg ccatttacaa tatcggattt gaaagaggga tcaacgagtg
     5161 gcgcaaattc ctgaaacccg gcggcttcct tgccgtttca gaggcttcgt ggtttactcc
     5221 cgaacggcct gccgaaatcg aagaattttg gaaagatgct tatcctgaga ttgatacgat
     5281 cccaaataag gtcttgcaga tgcaaaatgc cggctatgtc ccggttgcaa ccattgttct
     5341 tcctgaaaac tgctggatag aaaatttcta tgccccgttg gttaaggcac gtgaaatatt
     5401 ccttcagaag tatgccggca ataaaaccgc tgaagaattc atcgcctcag aactgcatga
     5461 aacccaatta tattttaagt acaaagcgtt ttacggctac gtattttata tcgggaagaa
     5521 gatctgatga tgatagaaga ggctgcctca atggattgag cagcctcttt tcctactctt
     5581 tattagcaga aaagttgtaa ataaccccaa agctgaacgt atgggccagg tcattgttga
     5641 agtattcctg gtttctatcc agcatcaggt tatgcagtcc atccacagaa ccattcaacg
     5701 caatctcaaa ttgctttgaa atggacaaca acactccggc ctggaccgac aggttgatta
     5761 aaaagccgga gggccaagcc tatggctaca gttggtcgca ccacaccctt gcaaaataca
     5821 tattgaaagg aaaggggtat cctgatctta tactgcctct ccccggaaga tgtgttatct
     5881 ttagcccaat atatcttgtc ggaataaatt agaccggtct tcaggaacat atgttcaata
     5941 ttctcttttc ttcccaggtg aacctgcagt ccgtaataag gagaataata ggcattatgg
     6001 taagtggcgc ccgaaacggc ctcaagtttc catgaaacaa tgaactttga aatacttttt
     6061 gtgtagttta tacactctgc atccttacat acgatattgt ggtagtcaat agcaattttg
     6121 accatgcttt tttgcgtaat gtcttctatt ttatcgattt cagggaagat cgcgggggca
     6181 tccgacatgt aatacctcat caaaccatta aaagtcctgt ttttcttcag gtagtaacgc
     6241 ccgtcactat tgacgacttc agtattcctg tcgtcaagtt ccagtaaatc ctgattatct
     6301 cttttgatga agtaccggtg agcatttctt attctgaaaa ggtcaatttc tccatcgacc
     6361 aaaaactcta aaaaacacca tttaacctct cctgacttat ttttaatttg tttcgaaaca
     6421 taatacttcc cattgatata cctgaaggca gaaatctcat cgggattgta aacccgggca
     6481 tcatctgatc cgatttctct gaacaggcag aaatcctgtt cagggcccat attccctgcc
     6541 ccaaacagcg tatctgcgtc gttggaaata atataggctg ctttggaact cccctgtcca
     6601 taaattgaac ccggaagcag gaagagaaga aaaatcagac tcgttaaaac tttcatgctc
     6661 cggtttggtc agaaaagcca attggtataa atgcgagtgc aatataatta atttaagctc
     6721 tccgcacaaa acaaaataac agaggctgta tcaaaagtaa taaaccacaa aggaacacca
     6781 aggatttcac gaaggaacac aaagtttatt atttgataat ctgatactta gtgcgccttc
     6841 gtgatttact ttgtgcggcc tttgtggtta aataatagcc ttttgagaca acctcagcat
     6901 aaagttcaaa cagaaaagaa tcctattcct tcactttgaa ctttgaacta tttgatcaca
     6961 cccagctctt tgccgacttt ggtgaatgca tcaatgcatt tatccaggtg gtggcgttcg
     7021 tgagcggcag atatctgtac acggatacga gccaggcctt tagccacgac cgggtaatag
     7081 aaaccaatga cataaatccc ctcggccagg agcttttcgg ccataacctg cgaaagcttg
     7141 gcatcgtaga gcatcaccgc gcagatagcg gatttggtcg gtttgatatc aaaaccggcg
     7201 gctttcatct tttccaggaa atagtcggta ttggcgtgca gcttatcctg taattcattg
     7261 gtttcggcca tcatgtcgaa catggctatg cctgcgccga cagcggccgg gatcagtgag
     7321 ttcgagaaaa gatacggacg ggagcgctgc ctgagcagtt cgatgatctc cctgcgcccg
     7381 gtggtaaaac cgccgatgcc accgccaaaa gcttttccga gtgtcccggt gatgatatcc
     7441 acctcaccgc gaatattgaa caattcggtt acacctcttc cggttttgcc taccacgccc
     7501 gctgaatgac attcgtcgac catgatcagg gcgtcgtatt tcttggccag ctcgttgatc
     7561 ttgtccattg gagccacgtt gccgtccatg gaaaacaccc cgtcggtaac aatgatcttg
     7621 aacctggcgg tttttgaggc ttcgatcagt ttctcttcca ggtcggccat gtcggcatta
     7681 ttgtaacgga accgctgggc tttgcaaaga cgaacgccat cgataatgga ggcatggttg
     7741 agggaatcgg aaataatagc atcctcttcg ccgaacaaag gttcgaaaac cccgccattg
     7801 gcatcaaaac aggctgcata aagaattgta tcctcggtgc caaaaaactc agcgatcttc
     7861 ttttcgagaa ttttgtgaat gtcctgggtt ccacagatga aacgtacaga cgacataccg
     7921 aatccatggg agtcaagggt ttcctttgct gccttcacca gtttcgggtg gttcgacagg
     7981 cccaggtaat tgttggcaca gaaattcaat acttcctggc ctccactgac cttgatttca
     8041 gctccctggg gagtgacaat gatccgctct tttttaaaca aaccggcttc ttcaatgctt
     8101 ttcagttcat tcttcagcca atcctgcaat tttccgtaca taatcctata ttttaaagtt
     8161 ttagtgttaa agaattaaca aagtggtgca aagataattt tttttaatat cagaaagatt
     8221 cctctccgcc ttccggcgga tcggaatgac gaataacaag catttgaaaa ggagggtatt
     8281 cgtcatccgg agcgaagcaa aggatctata aatctcaaaa tgaattattt aacatttcat
     8341 taacacatat gcaacatatt gcataatatt tttggtatcc caataaatat tcaccattat
     8401 gaaaaagctg ctaaaaatct ttctttggtt cgtccttgtt attgtcatta tcattgtcat
     8461 tgccgtgggt tacatccagc tcacatggaa taaaaccttt gatgccccct accccgacat
     8521 cacagccagt aacgacccgg cgatgatcga aaggggtaaa tacctggcct atggctggtc
     8581 gcactgcgcc gcctgccatg tgccggccga taaagtcagg gaggtggaaa acggcctcga
     8641 gatgccgctc atcggcggat gggatgagga aatagccggt tttggcactt tccgcgcccc
     8701 caatctcacc cccgatccgg agacagggat cggcaggatg accgatggcg aactggcccg
     8761 ggccatccgc tactgcgtaa aacacgacgg taaacttctt cctcctttca tggttttcca
     8821 gggattgagc gacgaagacc tgacggctgt catctctttc cttcgctcac aggaaccggt
     8881 taaaaatcaa gttgcacctt ccgattatgg gttcgtcgcc aaagctgtca ttgcttttgg
     8941 tttgcttaaa ccacaggggc cgaaatccac cccgccggca tccatccgtc cggatacaac
     9001 cgctgtttat ggagagtacc tggcaacgag cgtcggcaac tgcctcgggt gccacataaa
     9061 gatcgaccag gccgggaacc aggtgaaccc cgatttcgcc ggcggcggaa tttttcctcc
     9121 taccgctttt tctaaaggtt acggatttgt ttcaccgaac ctcacacccg acaaaaaaac
     9181 cggtgctatt gccgattgga gcgaagagca gtttgtaaac cgtttccagg gcggccgcct
     9241 gcacgagggg agcccgatgc cctggaacat gtattcccgg atgtcggtca acgatatgaa
     9301 agccatctac cggttccttc acacactgga tcccgtcggg ttttcagtgg aaaagaccgt
     9361 atatcagccg ggagagaaaa tgcctcagta ggttgcctgt ctttcgccca tgacccgctt
     9421 gaaatcggat gcgaggttac gggctaccgg caccggctct tcaaaacctg ccagggtcag
     9481 cttcaatccc tgcgaattgc ccgaaacttt ttcaatctta tcgaggttta cgatccatga
     9541 cctgtggcat ttgtaagcag ccgggatagg cgctatcaga tcttcggcat atttcatcgt
     9601 gtttctgatc agggttctga ccattttccc tttttttatg tacccggttg aaatataatt
     9661 cccttccgat tttataaaga ccaggtcatc aaataaaatc tccagttctt ctttgccccc
     9721 ttcggagaat atcttcagtg cagagaattg ggtttccacg tcgggccggg gatgcaaacc
     9781 ggatgtgatc attccggcgc tgaccgcatt cttccggtta agatagctct gtttgatcat
     9841 gacgatcgca atcaccggga tgatcccgat ggcaaccgta aatagctgga atatcagaat
     9901 attctggaaa gtaagcctga agccgatata aaagtgagaa tagaccaggt ttcccagccc
     9961 gatggtaaag aggataagca ggaagttcag catttcttta aagaccgtcc atttttcctc
    10021 cgagtaaact ttgggaaata ccaccggaat taaaatcatg tcgatcatga gaatgataaa
    10081 ggtcaccagg ccatatccgg ccaggatcag caatttgtcg ggcgactgga actcactcag
    10141 cccgaatggc tgaaaaacgg gcataaagaa cagaatgaaa atggagatcg gaaggatgat
    10201 cttccatttg ttctcctcgt atggatattg ctgcaaaaga tatttccgga tcattgattt
    10261 ttgtatgttt gaaaaagaat gacaaagtaa aattaaatgt tttcacaata agatgttgtt
    10321 gatccgtctc tccggccatt cctgcgcggg caaaaccagg ctggtcaacg ccctgccgaa
    10381 atatggcatc acgctcccca aagtgttgcg ctatacctca cgggcgcccc ggcccgaaga
    10441 aattgaaggt gaagattatt tcttccgtag ccgagaatac atcgaagcgc tgcccggcaa
    10501 agattttctg aaaggaccgg taaggaatat gctccatgcc ttcgacctga atgccatcgc
    10561 aaatcttctc ggcaatcata caggggtcat aattgagatc tatcccggtt tatggccgga
    10621 actggtcagc cggctccgga ttcggctcgg gaacaacctg aagaccgcat cggtattcat
    10681 gacggctgtc gatccggaac ggatcaggca gtttaaaaag aaagacgaaa tagcaggatt
    10741 tatatcgtca gaagtttata aaatgcttct tttcaggaac aaagatgaga tcgaagacat
    10801 taggatccgt gcttattcgg cggctctgga aattaccggg gccctgagcc cgcaaggcag
    10861 aaaagagtat gaccgaatca tcttttctgc cccggaaggg cctgatggtg aagaccaatg
    10921 gacccgcgga ccgcaacctg tagggcaggc tttaatggca ctgaataaat tcagagaatt
    10981 cctgtatgag tttgaaaaac cttattagaa tactatctca gccccagttt ctcaatcctg
    11041 tctcccacca ggatctgctt catctcatgt tccagcgccg ggaaatccgc ccttaaaacc
    11101 cgccggatga tcgctccctc tttcatcagg aagatttcgt cgcaggtttc acttaatgtg
    11161 gagaaaatat gcgaggatat aatgacggtt tttcccagtt ctttcatcct gaggataata
    11221 tcggcaatca ggatattgct ctgaatatca acaccgttga atggttcgtc gagaataaat
    11281 acctcatttt cctgcatcag gatcgccagc agcgccagct tcttcttcat ccccgtggaa
    11341 taggtcgaag catattgatt caacgggaga tcgaatatat ttttttcatg aatgttttcc
    11401 gcctttattc gcctggcatt gcacaataac cggatgtatt ccatcccggt gattttctgg
    11461 aagaaatagg gttccgtagg gagatagccc aggtggtctt taagatggcc gtaatccgta
    11521 ataatctcac catcatgctt ttccaatccg gcaatacatt tgaacaacgt ggttttcccc
    11581 gcaccgttct cccctaccaa tccgtaaaca caaccctttt cgaataccat tcgggcattt
    11641 tttacgacat gatgatcgcc ataatactta tttaaattct ttatctctat catgcaagaa
    11701 gggttttaag ccggatcgac gacttcctgt agaaaaacgg aatgacggcg agaagcaaag
    11761 gcggaaacca gaccgatacg accaggatga tgaactgcgg taaattcatc gggtgcggat
    11821 atgacgaata tttagccagg accacggcgc caaggtaaat atagccaaga agctggaatg
    11881 ctccgattat caatatctta tcggaataaa agaccgacag gaccaggctt accggaaggg
    11941 tcaggaatgt cgcgtaaacc agggctgttt ttattttccc gaacaggaaa ataccgggac
    12001 gactattgaa aatccagaca tagaattcat tttcaggatt gaggtaatag ctgaaacaaa
    12061 caaggaaaac caatatcagc gatgcaatgc caaggttgaa gttgccggag atcaccgcca
    12121 tgaccgtaag aaaccaggcc aggacaatca ccggaaatga gttccggaat cccacggtaa
    12181 attcaaatgg ccgcttcccg aagggggttg gaatggtaaa gccggaccgg ttgccaacaa
    12241 tggtcaacga cataaaaccg gcaaggaaca acgcggcaag ggcatgaaaa taatattttt
    12301 cgattacgag aaaagcggca aacggagcgg cgacgatgac attttcaagc atcctgacca
    12361 cccggtaatc ctttttcccg aaacagcttc tcaggaactg gttccgggcc ggatcgctta
    12421 atctggcaag aaaactgagg cttgcggcca ggtaaagata ttcggcgaaa gctgtttttt
    12481 caaagatgag gaccgatgag ccagtaaaag ctgccaggat cagcacgcag ccaataacgg
    12541 gatggatccc gaaagccgcc aggtgcctgt tgagcatttt aaactgtatc agaaaataaa
    12601 gtttcatttt gagccgggaa ttacgatttc atcggtgaat accattcctg tctcctccgc
    12661 cggcagttcc atcagccttg ccatttcttt gataatgtgt tcattatcca tcatattctc
    12721 ccgatccaac aaatgtacgg tcagttgttg ccgtaagtga aacagcttcc gcctggtatc
    12781 cggatcaaac atcccccggt tgaaaaaatt gctctcttcc gaatagttgt acgggacatt
    12841 ggtaactgac cggaagactt cccggcaggc gttgtccgcc tgttccgaat tcatcgaata
    12901 atgcgaccat tgatccataa atccttcgat cttccatttt ttccggatcg caggttctgc
    12961 cagttcgctt aggttgaata tcagcaaggg gtaaacctgg tagctgacgc ccaggtgagt
    13021 cagggccagg ttattcatga aatcgatggt attgcgcaac gtctcttccg tttcgcccgg
    13081 gaagccgacg acaaaggtca tcagtacccc tatcccctgc gcatcgaact gctcgatccc
    13141 tcttttcacc ctgtcaattt tctgcttttt gttcatccgg tcgagctgac cctggtcgcc
    13201 cgattcaacc cccatctttc ccatcatcag tcccgaatcg aggatcgctt tcatctcatc
    13261 ttccgaatat tcccctccgc gcataaaacc cgcccagtgg ttaagtcccg attttgaaag
    13321 tgttccgcag atttcgaaca atcttttccg ggtgatgaac acattatcgt cggtcacatg
    13381 gatcatggcg ggagtgctcc cggtccggga tttgaccatg ctcagctcct tgcagaggct
    13441 ctccggagag cgcaggaata ttttcgggaa aaggctgcag aaatcacaga accggcaacg
    13501 gtatgggcat ccgatcgatg tccggagcgg tattttttca ggcatctccg aaatcaaatc
    13561 ccatcttgtg aaatcctcat cgtaatcgac cttttcttcc accatgccgg taaaaacaaa
    13621 gccatcctcc cccggcaggc acaaattcgg aacatttgat aatcctccaa tcccccaatc
    13681 ctcaaatccc ttacctccct taccaccttt aaaaccctta aaaccctcac tctcaatcac
    13741 ctccagcacc cgcattaacg gccctattcc atgctgcgcc acgaccagga catccgcttt
    13801 tattgcggaa ttggaaggat ga
//
LOCUS       DAOQXS010000011        13346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5132_length_13346_cov_3.896246, whole genome shotgun sequence.
ACCESSION   DAOQXS010000011 DAOQXS010000000
VERSION     DAOQXS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_13346_cov_3.896246"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PKM34_00710"
     CDS             complement(<1..1053)
                     /locus_tag="PKM34_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNQ82127.1"
                     /translation="MKRIISLIAVAAVLFACSKQESDLPEDQGLAPGMIGEEKATLSV
                     TTTYFSPDITFTSATAGGIVTVTGGTINIVEKGVCYSTNPAPTVAGPKVSAGGGTGTF
                     TVSLTGLDSATTYYIRAYATRKNGVTKYGNELSFTTLIPPTFGTMTDIDGNVYTTITI
                     GTQTWMLENLRTTRYRNGDSIKHVTDNSEWANLRLATDKGGWCHYNNDPGNDAIYGKL
                     YNFYAATDTRNIAPEGWRVPTKEDWDILVLYLGADNIQYPTNIQYIGRKLKEAGIDHW
                     LAPNPADNVGGFTALPGGYRNETGVFNRMNSHGYFWSSSSQNGHPFYRELKFDIENVR
                     WGQYFGPAGYRPFGFSV"
     gene            1273..1620
                     /locus_tag="PKM34_00715"
     CDS             1273..1620
                     /locus_tag="PKM34_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ82128.1"
                     /translation="MSQKITYLTPQQEKLAKYAKALSHPVRIFVLNFLAANLDKCCYS
                     GDMAEELPIARSTLSEHLKELKKAGLIQGEINPPFIKYCINKENWEEARHLLSEFFAK
                     ERSEFSRKTCKND"
     gene            1624..1863
                     /locus_tag="PKM34_00720"
     CDS             1624..1863
                     /locus_tag="PKM34_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ82129.1"
                     /translation="MEIKILGTGCPKCKSLEKMAREVVEHNNINATITKVEDIMDIMN
                     YGVMSTPALVVNEKVEIKGRIPSAEEIINVLKKYQ"
     gene            1875..2303
                     /locus_tag="PKM34_00725"
     CDS             1875..2303
                     /locus_tag="PKM34_00725"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNQ82130.1"
                     /translation="MRMTIITLFFMSMIALQFPCNAQDNPAQTNNEEVKSSDLGVYYF
                     HFERRCNTCISVENNSKKALEELYPEPVKSGEYFFKSVNLDEAAGKEIGEKMGIGMQT
                     LIVVHGDKKVDITGEGFMYVDNYERLKAEVKKAVEKALKE"
     gene            2308..3006
                     /locus_tag="PKM34_00730"
     CDS             2308..3006
                     /locus_tag="PKM34_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNQ82131.1"
                     /translation="MEFLQSILDNSQYAALTAFILGLMTAISPCPLATNISAIGFISR
                     NLENRRKVFLSGLIYTLGRVISYTGLAIIIYFGASKVDVAGLFQGWGEKLLGPILVLI
                     GLSMLDVIKIKFPGFSKLSDRIGEKGKGSYWSSLLLGMIFALAFCPYSGVLYFVMLIP
                     ISIASPSGLFLPVVFAVATGLPVIIFAWLLAFAVGNVGKWYNRIKIFELWFRRVVAVV
                     FIGVGVYYIIMFFF"
     gene            3060..4400
                     /locus_tag="PKM34_00735"
     CDS             3060..4400
                     /locus_tag="PKM34_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ82132.1"
                     /translation="MENKKELKILLWITVIFGVAFFLPIESVRFTTAIDATFDLVKWY
                     AREHVILCLLPAFFIAGVISVFVSQGSVLRYFGANAKKWLAYSVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARTIGAVSFSVI
                     IGIAMSLIYRKEEKIKREEQMNITVPPEKRPMWQTSFHFFILVLILVFANWGSPASGD
                     TSGAWYLIWHYKWYITGFFSLLLGFSLVYILKIKWQWVLAAFIATALSAFLSTYYIHN
                     EKLAPLVPMLIGIASLSIITLLDKQDEDNREWTISTWGFAKQILPLLAIGVVTAGFLL
                     GSTHDGKTIAGIIPNEWIQWLVGGNSVFSNFFASVVGAFMYFATLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVMGTQKTVMYVVLVVIMATISGLIYGTI"
     gene            4411..4686
                     /locus_tag="PKM34_00740"
     CDS             4411..4686
                     /locus_tag="PKM34_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ82133.1"
                     /translation="MAIQIVILCPGSKCKKCRRMIRQVEEAVLESGLEAEVKILDTLD
                     DLMKYNTYVLPALVINGKMLARGYVPEKSMIIKEMNKLPDNGKKYES"
     gene            4676..5089
                     /locus_tag="PKM34_00745"
     CDS             4676..5089
                     /locus_tag="PKM34_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ82134.1"
                     /translation="MSLEIEKYGFVINDLRHVSGKECLEILKTDGFILDVRPDFELTR
                     LIDHEHILYYPYDEIREHFRKLPHDTWLILADAVGLRSKEVAFFLKENGFSNILHLAG
                     GVVEWERDGLPVTLDKSRRLTGSCMCQLKRREIKK"
     gene            5142..5555
                     /locus_tag="PKM34_00750"
     CDS             5142..5555
                     /locus_tag="PKM34_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82135.1"
                     /translation="MKKHITFMMAVMLTISAGMIFGQSNKTSTEVLYFKANLCKCKAG
                     ACNALQADVDSVIVKYFQKENIEFKVIKLADEANKELVSRFNAKSQTVVIISKKKKKE
                     NVIDASAIVADYSKTKDKVRFEAEMRKKISESLVF"
     gene            5587..5961
                     /locus_tag="PKM34_00755"
     CDS             5587..5961
                     /locus_tag="PKM34_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82136.1"
                     /translation="MSESNKPGFIMCVCTGKCPGFSQMDIWDFINRVRIELPVEYGFI
                     HPQLCEEDGDRFLLDFLKAHRKVVIGGCAPNMQMKLFRDAFKESGLEISTDLIPIDVR
                     DMTTDQAIEKVSAALQKMEEQK"
     gene            6244..7101
                     /locus_tag="PKM34_00760"
     CDS             6244..7101
                     /locus_tag="PKM34_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNQ82137.1"
                     /translation="MIEKYTILPCNGLDKCAGVISGELAIRLCESGKNELICPVFYRV
                     SESRYNKMANVRPLLVIDGCQTRCASRLAAEKNLKVSKKLTITEEAKNHGIELKSNLR
                     LQDQEKALVEGIFKELNEEGEKVIMAPVEAITSLHFEYETYQFGKFLFRVPSNPGIYF
                     NENDGWAYIYGNHARVGVTDFVQQNLSDILYFTPPEIGDEIDQFGEAGDIESSKSVFE
                     LISPVSGKVVAINEELVKKPEMINENPYEEGWMAELELTDFESDKELLIGFEEYLELM
                     KRKVQELNE"
     gene            7094..7498
                     /locus_tag="PKM34_00765"
     CDS             7094..7498
                     /locus_tag="PKM34_00765"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNQ82138.1"
                     /translation="MNKKVKVIPCSGIGKVYGLIARESALKTVFDLLPEKSETVCLAY
                     IVTGDKEAREKIEGFACITVDGCPKMCASKNVSIAGGNVMEEIKVLDTVKEHKGKKFG
                     SPTQLDEDGELVTDEIAAKIAKTINELNEGGK"
     gene            7499..7948
                     /locus_tag="PKM34_00770"
     CDS             7499..7948
                     /locus_tag="PKM34_00770"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNQ82139.1"
                     /translation="MDELKVGIISCSGEECLGGTISRLATRKVLEELKMISTVTLCLP
                     LYLAGGEEERNFANVYPTLSVDGCDKLCAKRSTEKYSGKINGSIDVSSIIGKENALNT
                     KTVRNKDLTAEHIAMAEEVASEIVKMISNLPPDSYSGKTFHGCACGS"
     gene            8001..8342
                     /locus_tag="PKM34_00775"
     CDS             8001..8342
                     /locus_tag="PKM34_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82140.1"
                     /translation="MILLLILMPACHKEDKNPVVNPSEKLALDSLVATNKHIVTWEEI
                     QVRAYARGENLNFEWYTNHGSMIAVDSATVLYWACPSCEGLNIIECTVSNDYGSISDT
                     IMIQVDPENGL"
     gene            8359..9267
                     /locus_tag="PKM34_00780"
     CDS             8359..9267
                     /locus_tag="PKM34_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82141.1"
                     /translation="MIFALFSLNGPGQCCSAGNPLGSNGINDGLPVKEWQFLTSYKHS
                     LSKNYFHKDHQVEAPVVEKSYYDYQSISVAYGILKWLNASAEIGYFYNKTQELEPGQS
                     TDQIRAGGLGDLALNLRVLPVKTLRRTSQFIASGGIRIPVGAFEKEIDGVTVPVSLQP
                     SSGALKINTSVYYSMPLFRPEFSMNSFAFYEYSNTIEEGYFIYKYADFFQYSIGGQYV
                     YNKRLSILLNMKYEFRGKDHRENEAVVESSGSHLVLINPQVTYNIKRGWSLMVLADLP
                     LYKYVNGKQLTNSYAVQCSLYKRITF"
     gene            9354..9689
                     /locus_tag="PKM34_00785"
     CDS             9354..9689
                     /locus_tag="PKM34_00785"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ82142.1"
                     /translation="MPQAKTELFETELQTTSMFLKALSHPARLMIVKFLADCKSCYTG
                     DITSELPLSRTTINQHLDELRKAGLILGHITGNKTSYCINQERIAEMKSIISAYLDEL
                     DISVPFKCE"
     gene            9686..10477
                     /locus_tag="PKM34_00790"
     CDS             9686..10477
                     /locus_tag="PKM34_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82143.1"
                     /translation="MIIKIYKTKSRKEISMKTRLFFLLLVMAIISLQASATVKSDLEA
                     ANKNGNSVFLVVTDPNITGTDQALDIANKAKTSVTKSIVLQMNRSDAANTELVTKYRL
                     AGAPLPLILVIASNGVVTAGYALAQATPELLVKAVPSAKKSEVLKALSDGKSVFIVVT
                     GKSMAEKTTIMNTCQQACVEMENNAKMIEISLDDPKEKQFLTELKVNMAAAEPTTYVI
                     NSKGQMTGTFTDDVNSTTLVASAKKVAASGCCPSGSGKSCGPAKK"
     gene            10487..11704
                     /locus_tag="PKM34_00795"
     CDS             10487..11704
                     /locus_tag="PKM34_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82144.1"
                     /translation="MKVIKLVWLELGQRKTQLMSGILAITLGISVIVAIRSISLVSEK
                     AVAINLDNLGANIMVLPQGATIDNYYSADIDAPTIPEEYVERILTSSLQGVDNLSPKL
                     TRRVDIGDYKFVLTGILPSNELASKPLWQSAGLFGAGLAASCGENPQNESLGYKDEKL
                     QRKSIDSLAMADCLLGYSAAQKLGLKEGGVLTIMGNELKVARVLTETGTIDDDRVFAH
                     LRSVQNILGIHDQVSAIEIMGCCNAISDGLLGQLRNILPDTRITTIGQIVSTQIKTNQ
                     LMNQISLIILIIILIVGGISIGNYMWANVNERRKEIGILRMIGFHKSSIYSMLLIKAV
                     FMGVVGGLAGYLAGALIAWFLGPTLAGIIVVPIPLLLLYSVLLAAGIAVVGSLLPAYF
                     ASRFEPYSNMQEI"
     gene            11701..12375
                     /locus_tag="PKM34_00800"
     CDS             11701..12375
                     /locus_tag="PKM34_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82145.1"
                     /translation="MTTILSTNALSKHYFHRDGLIMALDDVSISVSEGSFVTITGPSG
                     SGKTTLLMALSGLIRPTSGKIYFKEQVLETDNDTALVEFRKRYTGFIMQNFSLIPYMT
                     AIQNVMLPLSLNSISEESRKHRAVELLELAGLGNRMNHYPRELSAGQQQRVAIARALA
                     NKPAIIFADEPTGNLDPMLSAEILNFLKEINEKNRITIFMVTHSPAAGAYGNVSIKLR
                     DGRIEN"
     gene            12415..>13346
                     /gene="arsB"
                     /locus_tag="PKM34_00805"
     CDS             12415..>13346
                     /gene="arsB"
                     /locus_tag="PKM34_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNQ82146.1"
                     /translation="MPSKSRLKFLDRYLTLWIFLAMFAGVFMGWAFPSVAGFWDSMSS
                     GTTNIPIAIGLIVMMYPPLAKVKYEELGDVFRNVKVLSLSLVQNWIIGPVLMFLLAII
                     FLQFNIEYMYGLILIGLARCIAMVIVWNDLAKGDRQYAAGLVAFNSIFQVLFFSVYAW
                     FFIAVLPGWFGLPVNDAVEQITIGQIAKSVFIYLGIPFIAGFLTRFILIRVKDKTWYE
                     TKFVPKISPLTLIALLFTILVMFSLKGEYIVKIPLDVVRIAIPLVIYFVIMFLVSFFM
                     SMKVGANYEQSTTLSFTAASNNFELAIAVAIAVFG"
BASE COUNT         3761 a         2788 c         3168 g         3629 t
ORIGIN      
        1 gacagaaaat ccgaatggcc tgtagcccgc cgggccgaaa tactggcccc agcgcacatt
       61 ttcaatatcg aatttcagtt cacggtagaa cggatgacca ttctgactgc ttgagctcca
      121 gaaatatcca tgggaattca tcctgttgaa tactccggtt tcgttgcggt atcccccagg
      181 caatgccgta aatcctccga cattatcagc gggattaggt gccagccaat ggtcaattcc
      241 tgcttcttta agcttacgtc caatatattg tatgtttgtc ggatactgaa tgttatcagc
      301 gcccaggtaa agtaccagga tatcccaatc ctccttggta ggcacgcgcc aaccctcggg
      361 agctatatta cgtgtgtcgg ttgctgcata aaaattgtac aacttaccgt agatcgcatc
      421 atttcccggg tcattattgt aatggcacca gccgccctta tccgttgcta gcctcaggtt
      481 agcccattct gagttatcag tgacgtgctt gatgctatcc ccgttgcgat accgggtagt
      541 ccggaggttt tccagcatcc aggtctgtgt gccaattgtt atcgttgtgt aaacattccc
      601 gtcgatgtcg gtcattgtgc caaatgtagg cgggatcagg gtggtaaacg acaactcgtt
      661 gccatatttg gtcacgccgt tctttcttgt ggcataggcg cggatgtaat aggtagtggc
      721 ggaatccagc ccggtcagag aaacggtgaa ggtgcccgtt cctcctcctg cggaaacttt
      781 cgggcctgca acggtaggag cagggttcgt gctgtagcat accccttttt caacaatatt
      841 gatagtaccg cctgttacag tcacaatccc cccggccgta gctgaggtga acgtaatatc
      901 cggcgaaaaa tacgtcgtgg tcacactcag cgtggccttt tcttccccga tcatgcccgg
      961 ggccagcccc tggtcttctg gaagatcgga ttcctgcttg ctgcaggcga acagcactgc
     1021 tgcaacggct attaaactga tgattctttt catggtgttc tgtttttaag gttagtaatg
     1081 tatatgaatc aatagacctg ttctttttca attttaatga ctggatttgc ctgcacgatt
     1141 tgtaccatca aaatccggat gtaaaattaa attgtgaaag agcgcttgta aataaccctt
     1201 taggggaata tttttttgct ttatccactc cgtattcaaa acatttcgta tttttgcgaa
     1261 atacgaaatg caatgagcca gaagataaca tatctcacgc cacagcagga gaagcttgca
     1321 aagtatgcca aagccctttc acatcctgtg cgtatcttcg tgctgaattt ccttgccgcc
     1381 aacctcgata aatgctgcta ttcaggcgat atggccgagg agttgcccat cgcacgctct
     1441 accttatccg aacatcttaa ggaacttaaa aaggccggat tgatccaggg tgagatcaat
     1501 ccgcctttta tcaaatactg tatcaataaa gaaaactggg aagaagcccg ccatcttcta
     1561 agtgaatttt tcgcaaagga aagaagcgag ttttcccgga aaacctgtaa aaacgactga
     1621 aatatggaaa ttaaaatcct gggcacaggc tgccccaaat gcaagtcgct cgaaaaaatg
     1681 gcccgcgagg tggtcgagca taataatatc aacgcaacca tcaccaaagt ggaagatatc
     1741 atggatatca tgaattatgg cgtgatgtcg actcccgccc tggtggtaaa tgaaaaggtc
     1801 gagatcaaag gccgaatccc ttctgcggaa gagattatca atgtcttaaa gaaataccaa
     1861 taaaacgtat gaatatgaga atgacaatta taaccctgtt tttcatgtcc atgattgcct
     1921 tgcagttccc ctgcaatgca caggacaacc cggcacaaac gaacaatgag gaggtaaagt
     1981 cctcagattt aggagtatat tattttcatt tcgaaagaag gtgcaatacc tgtatttctg
     2041 ttgaaaataa ttcgaagaaa gccctggaag aattataccc ggagccggtt aaatcaggcg
     2101 agtatttctt taagtctgtc aacctggatg aggctgccgg aaaagaaatt ggtgagaaaa
     2161 tgggcatcgg gatgcagaca ctgatagtgg tgcatggcga caaaaaagtt gatatcaccg
     2221 gcgaaggatt tatgtatgtc gataattatg aacggcttaa ggcggaagta aagaaagctg
     2281 tcgaaaaagc gttgaaagaa tagaccgatg gaattccttc aaagtatcct ggataattca
     2341 caatacgctg ccctcactgc atttatcctg ggattaatga cagccatcag tccctgcccc
     2401 ctggcaacga atatttccgc catcggattt atcagccgga acctggagaa tcgccgtaaa
     2461 gttttcctta gcggattgat ttatacgctg ggaagagtca tttcttatac cggtctggca
     2521 attatcatct atttcggcgc cagcaaagtg gatgtggccg gcctgttcca gggatgggga
     2581 gagaagctgc tcggacccat tcttgtcctg atcggattat ccatgctgga tgtgattaag
     2641 ataaaatttc cgggtttttc aaaactgagc gatcgaattg gtgaaaaggg aaaagggagc
     2701 tactggagtt cccttttact gggaatgatc tttgcccttg ctttttgtcc atacagcggc
     2761 gttctctatt ttgtcatgct gatcccgatc agtattgcca gcccaagtgg tctcttcctg
     2821 ccggtcgttt ttgcagtcgc aaccggattg cctgtcatca tttttgcctg gttgctggca
     2881 tttgctgtag gaaatgtagg caaatggtac aaccggatca agatctttga actctggttc
     2941 agacgtgtag tcgctgtggt ttttattgga gttggggtgt attatatcat aatgttcttt
     3001 ttttgaaaaa tttttagttc atcatttcgg gttttagcga aatgtttgat aatataggaa
     3061 tggaaaataa aaaagaatta aagatccttc tttggatcac cgttattttc ggggtggcgt
     3121 tttttctgcc cattgaaagt gttagattca caacggccat tgatgccaca ttcgacctgg
     3181 tgaaatggta tgcccgtgaa catgttatcc tatgtttatt acccgctttc ttcattgccg
     3241 gtgttatttc ggttttcgtc agccagggct ctgtattgcg ttattttggc gctaatgcaa
     3301 aaaaatggct ggcttattct gtagctgccg tatccgggac catactggcg gtatgttcct
     3361 gtaccattct gcccttgttt tccagcattc acaagcgcgg tgctggtctt gggcctgcta
     3421 ttgctttttt gtattcaggg cccgccatca atatcctcgc cattatcctt acggcccgga
     3481 tactcggttt tgagatgggt gttgcccgga caatcggggc ggtatcattc agcgtgatta
     3541 ttggaatcgc tatgtcatta atctatcgta aggaagagaa gataaagcgg gaggaacaaa
     3601 tgaatattac tgttcctccg gaaaaaagac cgatgtggca gacttctttc catttcttta
     3661 ttttggtact gatccttgta tttgccaact ggggaagtcc ggcttccggc gacacatcag
     3721 gggcctggta tctcatctgg cattacaaat ggtatattac cgggttcttt tctttattgc
     3781 ttggcttttc cctggtttat atcctgaaaa tcaaatggca gtgggtgttg gctgctttta
     3841 tcgcaaccgc attatctgcc ttcttatcaa cgtattacat acataacgag aaacttgccc
     3901 cattagtccc gatgctcata ggtattgctt ctttaagcat cattacttta ttggataagc
     3961 aagatgaaga caatagggaa tggaccatct cgacctgggg atttgctaaa caaattctgc
     4021 cactgcttgc aattggcgtg gttacggctg gattccttct gggctcaaca catgatggga
     4081 aaacgattgc cggtattata ccgaatgaat ggatacagtg gttggttggc ggaaattcag
     4141 ttttttctaa tttctttgca tcggtggttg gtgcgttcat gtattttgcc accctcaccg
     4201 aagtgccaat tctgcaagga ctcatagctt cgggtatggg taaagggcct gcactggcgc
     4261 tgctcctcgc cggcccctca ttatcgttac cgaatatgct ggttattagg ggagtgatgg
     4321 gaacacaaaa aaccgtaatg tatgttgtgc tggtcgtaat tatggccacg atcagcggat
     4381 tgatttatgg cacaatctga ctttattcaa atggctatac agatcgtcat attatgtccc
     4441 ggaagcaaat gcaaaaagtg caggcgaatg atccgccagg ttgaagaagc agtgctggaa
     4501 tcggggcttg aagcagaagt taagatcctg gacacactgg acgacctgat gaaatacaat
     4561 acatacgtgc tgccggccct ggtgatcaat ggcaagatgc tggcccgggg atatgtaccg
     4621 gaaaaaagta tgatcataaa ggaaatgaat aaactgcccg ataacggaaa gaaatatgag
     4681 tcttgaaatt gaaaaatacg gatttgtgat caacgaccta cggcatgttt ccggaaagga
     4741 atgcctggaa attttaaaga ctgacgggtt tattttggac gtcaggcctg attttgaact
     4801 taccaggttg atcgaccacg agcacatcct ttattaccct tatgatgaga tcagggagca
     4861 cttccggaaa ctgccccacg atacctggct cattcttgcc gatgcagtcg ggctcaggag
     4921 taaggaagta gccttctttc tcaaagagaa tggattttca aatatccttc accttgccgg
     4981 tggagtggtt gaatgggaac gagacggtct tcctgttaca ctggataaaa gccggagact
     5041 gacaggatct tgcatgtgcc agctaaagcg cagggaaatc aaaaaatgaa agatgccatc
     5101 acgacttaac aatgagttat taaccaaata aattctaaaa aatgaaaaag cacattacat
     5161 ttatgatggc agtcatgcta accatttcgg caggtatgat ctttgggcag tcaaataaga
     5221 cgagtacaga ggtgctgtat tttaaagcca atttgtgtaa atgcaaagcc ggggcgtgca
     5281 atgctcttca ggctgatgtc gattccgtca tcgtgaaata tttccaaaag gaaaatattg
     5341 agttcaaagt tatcaaactg gccgatgagg caaataagga attagtatcc agattcaacg
     5401 caaaatcaca gaccgtagtg attattagta aaaagaagaa gaaggaaaat gtcattgatg
     5461 cctctgctat agttgcagat tattctaaaa caaaagataa agtaagattt gaagctgaaa
     5521 tgaggaaaaa aataagcgag agtcttgtgt tttaataaaa aaaacaatct gtataatatt
     5581 agatatatgt ctgaatcgaa taaacccggt tttatcatgt gcgtttgcac aggcaaatgt
     5641 cccggattct cccagatgga tatatgggat tttataaaca gggtaaggat cgaactgccg
     5701 gtagaatacg gatttataca cccgcaactt tgtgaggaag acggggaccg gttcctgctg
     5761 gattttttaa aggctcacag gaaagtagtt ataggcggct gtgctcccaa tatgcagatg
     5821 aagcttttca gggatgcatt caaagaatcg ggcctggaaa taagcacgga tctaatccct
     5881 atcgatgtgc gggatatgac aactgaccag gccatagaaa aagtcagtgc ggcgctgcaa
     5941 aaaatggagg agcagaaatg agcgaaacca cttttatggg tgtcccgcgc aataaaatcg
     6001 actggaagcc tgccattgat tattcgaaat gcgattactg catggaatgc gtgaaattct
     6061 gccctcacag ggtattcgaa gtcagggaga ccgaagaaat tagattgatt gtaaggaacc
     6121 ctcataattg cgttgttttc tgcagggctt gctcgaaaat ttgcggtccg gatgcactgt
     6181 catttccgga taaaaatgca actacactaa gaattaagga gattagaaag gagatggaag
     6241 gccatgattg aaaaatatac catccttccc tgcaacgggc tggataaatg cgcgggtgtc
     6301 atttcaggag aattagccat caggctctgt gagagtggaa aaaatgagct gatctgcccc
     6361 gtattttaca gggtttctga atccagatac aacaagatgg caaatgtgcg ccctctcttg
     6421 gttatcgatg gatgccaaac gcgttgcgcc agcaggcttg ccgctgaaaa gaacctgaaa
     6481 gtttcaaaga aactgaccat caccgaagaa gccaaaaatc atgggattga attaaagagc
     6541 aatctcaggc ttcaggacca ggaaaaggct ctggttgaag gtatttttaa agaattgaat
     6601 gaagaagggg agaaggtgat aatggcaccg gtcgaagcaa ttacctcttt gcatttcgaa
     6661 tacgagacat atcaattcgg taaattccta ttcagggtcc caagtaatcc cgggatttat
     6721 ttcaacgaaa atgacggctg ggcttacata tacggaaacc acgcccgggt tggcgttacc
     6781 gattttgtac aacagaatct gtccgatatc ctctatttta cacctcctga aatcggagat
     6841 gaaatagacc agttcggaga agcaggtgat attgaatcgt caaagtcggt ctttgaattg
     6901 atttcgcctg tcagcggcaa ggtcgttgca attaatgaag agcttgtaaa aaagcctgaa
     6961 atgatcaatg agaatcctta tgaagagggt tggatggcgg aacttgaatt aaccgatttt
     7021 gaaagcgata aggagctttt gatcggattt gaagaatacc tggaattaat gaaaagaaag
     7081 gtgcaggaat taaatgaata aaaaagtaaa agtgataccc tgtagcggta tcggtaaggt
     7141 ttatggcctc attgcaagag aatctgcttt gaaaacggtt ttcgacctgc tccctgaaaa
     7201 atcagagacg gtttgtctcg cctacattgt aaccggcgat aaagaagcga gagagaaaat
     7261 cgaaggtttt gcatgcatta ctgttgatgg gtgccctaaa atgtgtgctt cgaagaatgt
     7321 ttcaatagcc ggcgggaatg tcatggaaga aataaaagta ttggatacgg ttaaggagca
     7381 taaaggaaaa aaattcggat caccgacgca gcttgatgaa gacggcgaat tggttacgga
     7441 tgaaattgct gcgaagattg caaaaacgat taatgaactg aatgaaggag ggaaataaat
     7501 ggacgagtta aaagttggaa ttatttcatg cagtggagaa gaatgcctcg gtggaactat
     7561 ttcgaggctt gccacaagaa aagttctcga agaactgaaa atgatcagca ctgtgacact
     7621 ttgccttccc ctgtatcttg ccggtggtga agaagaaagg aatttcgcaa atgtataccc
     7681 gacactgtcg gtagatggtt gcgacaagct atgcgccaaa agaagcacgg aaaaatacag
     7741 cggaaagatc aatggctcca tagatgtttc cagtatcatc ggcaaggaga atgcactgaa
     7801 cacaaaaacc gtcaggaata aagacctcac cgcagagcat attgctatgg cggaggaagt
     7861 tgccagtgag attgtgaaaa tgatcagtaa ccttcctcct gattcttact cgggtaaaac
     7921 tttccatggc tgcgcctgcg gaagctaagt agaactatat ctatcattta catattctta
     7981 tggaaagaaa tttctgcttg atgatattgc ttttaatact catgcccgct tgtcataaag
     8041 aggataagaa ccctgttgtc aatccttcgg aaaaattagc cttagacagc ctggttgcca
     8101 cgaataagca tatcgtaacc tgggaggaga tccaggtaag agcctatgcg cggggagaga
     8161 atctcaactt tgaatggtat accaatcatg gaagtatgat cgctgtcgat tcagccaccg
     8221 tgctttactg ggcctgccct tcctgtgaag ggctgaatat catcgaatgc accgtttcaa
     8281 atgattatgg cagtatttct gatacaataa tgatacaagt tgatccggag aatgggttat
     8341 aaatttctct tgttaatcat gatctttgcc ctcttttcat tgaatgggcc cggtcaatgt
     8401 tgctctgccg gaaatccact gggcagtaat ggcatcaacg atggtttgcc cgttaaggag
     8461 tggcagtttc tgacgtcata caagcatagc ctttcgaaga attattttca caaggatcac
     8521 caggtggaag cccctgtggt cgaaaagagt tattacgatt atcagagcat ttctgtagct
     8581 tatgggattc tgaagtggtt gaatgccagt gccgaaatcg gttattttta caataagacc
     8641 caggaactgg agcctggcca gtcgacggat cagatcaggg ccgggggcct gggcgacctt
     8701 gctcttaatc tcagggtgct gcctgtaaaa actctcagga gaacatccca gttcattgca
     8761 tcgggtggga tcaggatccc ggtcggggcg tttgaaaagg aaattgacgg agtgacggtc
     8821 ccggtttcct tacagccatc atcaggggca ttaaagatca atacaagcgt atattattcc
     8881 atgcctctct ttcgtcctga attttcaatg aattccttcg ctttctacga atacagcaat
     8941 acaatagaag aaggttattt catttataaa tacgcggact ttttccagta cagcatcggg
     9001 gggcaatatg tctataataa gcgactgtcg attttactga acatgaagta tgaattccgt
     9061 ggcaaagatc acagagagaa tgaagcagtg gtcgaatcct ccggcagtca cctggttctg
     9121 atcaaccctc aggtgacgta taacattaaa cggggatgga gtttaatggt attggctgat
     9181 ttgccgctat acaaatatgt gaacgggaaa cagctaacga atagttatgc tgtgcaatgt
     9241 tcattgtata agaggatcac attctaagcc aaaattcaat ctaaaccata aaaaaattaa
     9301 cgtatttata cgtcaattca aaaatgatgt atttttacgt caaaattaat tttatgccac
     9361 aagctaaaac cgagttattc gaaaccgaat tacaaaccac ctctatgttc ttaaaagcgt
     9421 taagccatcc ggccagactg atgattgtca aattcctggc cgattgcaaa agctgttata
     9481 ccggagatat cacttctgag cttccgctga gcaggactac catcaaccag catctggatg
     9541 aacttagaaa agcaggactg atcctgggac atattacagg aaataaaacc agttattgca
     9601 tcaaccagga acgtattgct gaaatgaaat ccattatatc agcgtacctg gatgaactgg
     9661 atatttcggt tccttttaaa tgtgaatgat aattaaaatt tataaaacca aatcacgtaa
     9721 agaaattagt atgaaaacac gtttattttt ccttttactg gtcatggcaa tcatcagcct
     9781 gcaagcttct gcaaccgtaa aaagcgacct ggaagcagca aacaaaaatg gtaattccgt
     9841 tttccttgtg gttaccgatc cgaatattac cggaacggat caggcactgg acattgcaaa
     9901 taaagccaag acaagcgtta ccaaatccat tgttctgcaa atgaaccgtt ctgatgcagc
     9961 caacacggag ttagtcacca aataccgtct tgccggagcg ccattgccgc ttatccttgt
    10021 aattgcttcc aatggtgtgg taaccgcggg atatgcgctg gcccaggcca cacctgaatt
    10081 actggtaaaa gctgtcccat cagcaaaaaa gtctgaagtg ctgaaggccc tgagtgacgg
    10141 taaatctgtt ttcattgtcg ttactggcaa atcaatggca gagaagacca cgatcatgaa
    10201 tacatgtcag caagcgtgcg ttgaaatgga gaataacgcc aaaatgattg aaataagcct
    10261 tgacgatcca aaggagaaac aatttctgac ggaactaaag gtaaatatgg cggctgcaga
    10321 acccactact tatgtcatta actccaaggg ccagatgacc ggtactttca ctgacgatgt
    10381 aaactccacc acactggttg cttcagctaa gaaagtggcc gcatcgggtt gctgcccttc
    10441 gggtagcgga aaaagctgcg ggcctgcaaa gaaataatct gatcctatga aagttattaa
    10501 attggtttgg ttggaactcg gacagagaaa aacacaacta atgtcgggga tcctggcaat
    10561 aacattaggg atctcagtca tcgtagccat caggtcaatt tcgctggttt ccgaaaaagc
    10621 agtggcgatc aacctggata accttggcgc caacataatg gtgcttcccc agggggccac
    10681 gatcgataat tattattcag ccgatattga tgctccaacg attcctgaag agtatgttga
    10741 aagaatcctc acttcctcat tgcagggagt agataacctc agcccaaaac ttacccggcg
    10801 tgtcgatatt ggtgattata aatttgtatt gaccggcata cttccttcga atgaactggc
    10861 atctaaacca ctatggcaat cggccggact ctttggagcc gggctcgcag caagttgcgg
    10921 tgaaaatcca caaaatgaat cccttggcta taaagatgaa aaactgcaac gcaagtctat
    10981 tgattcgctg gcaatggccg actgcctgct tggttattca gcagctcaaa aacttggcct
    11041 gaaagagggg ggcgttttaa caattatggg taatgaactg aaggttgcca gggtattgac
    11101 agaaaccggg actattgatg atgacagagt ttttgctcac ttaagatctg tgcagaatat
    11161 cctgggtatt catgaccagg tcagtgccat tgagatcatg ggttgctgca atgcgatatc
    11221 tgatggctta ttgggtcagc tcagaaacat tttgcctgac acgcggatca ccaccatcgg
    11281 tcagatcgta tccacacaaa ttaaaacgaa ccagttgatg aaccagattt cgctcatcat
    11341 tctgatcatt atcctgatcg tgggcgggat ctccattgga aactatatgt gggccaatgt
    11401 caatgagcgt agaaaagaga tcggcatcct gcgaatgatc ggctttcaca agtcttccat
    11461 ttattcaatg ctgctgatta aagcggtttt tatgggagtg gtcggaggtt tggccggtta
    11521 ccttgccggc gcattgattg cctggttcct gggtcccaca ctggctggca tcattgtcgt
    11581 gcccattccg ttactcttac tctactcggt actgcttgca gcgggtatcg ctgtcgtcgg
    11641 ctccttgctt ccggcttact ttgcctcacg gtttgaaccc tattcaaata tgcaggaaat
    11701 atgacgacga tcctcagtac taatgcctta tcgaagcatt attttcacag agacggattg
    11761 attatggcgc ttgacgatgt atccatttct gtttctgaag gctccttcgt caccattacc
    11821 ggaccctccg gatcaggaaa gaccacgctg ttaatggcat taagcggatt aatccgtccg
    11881 acctcgggga agatttattt taaggaacag gtgctggaga cggataacga cacagcgctg
    11941 gtcgaattcc gtaaaagata caccggtttt atcatgcaga atttttcgct gatcccatat
    12001 atgaccgcga tacaaaatgt aatgcttcct ctgtcactca atagtatatc ggaagaaagc
    12061 cggaaacaca gagcggtaga actcctcgaa ctggctggac ttggaaaccg gatgaaccat
    12121 tatccacggg agttatcagc cgggcaacag caaagagtgg ccattgcgag ggcgctggct
    12181 aataagccgg cgattatatt tgctgatgaa ccgacaggga atctggatcc catgctatct
    12241 gcggagattt tgaattttct taaagaaatt aatgagaaaa acaggatcac tatttttatg
    12301 gttacgcaca gtccggccgc cggtgcctac ggtaatgttt caattaagct gagggatggc
    12361 aggattgaaa attagcaagt ttaataaata atgaatttca tcaagaattg aaatatgccg
    12421 tcaaaatcac gattaaaatt ccttgaccgt tacctgaccc tctggatttt cctcgccatg
    12481 tttgcggggg ttttcatggg ttgggcattc ccctccgttg ccggattctg ggacagcatg
    12541 tcgagcggga caaccaatat ccctattgcc attggactga tcgtcatgat gtacccgcca
    12601 ctggcgaaag taaagtacga agaactcgga gatgttttca gaaacgttaa ggtactgagc
    12661 ctgtcgcttg ttcagaactg gatcatcggg ccggtcctga tgtttcttct ggcgataatt
    12721 tttctgcaat tcaacatcga atacatgtac ggtctcatcc tgatcgggct ggcccggtgt
    12781 atcgctatgg tgattgtatg gaacgacctg gccaaaggcg accggcagta cgctgccgga
    12841 ctggtggcat ttaattccat cttccaggta ttgttctttt cggtttatgc ctggttcttt
    12901 attgcggtcc tcccaggctg gttcggctta ccggtaaatg acgctgttga gcagatcacc
    12961 atcggccaga ttgccaaaag cgtgttcatc tacctgggga tcccgtttat cgccggcttt
    13021 ttaacccgat ttatcctgat cagggtgaag gataagacct ggtacgaaac gaaatttgtc
    13081 ccgaaaatca gtcccttaac actgattgca ttgttgttca ccatcctggt gatgttctcc
    13141 ctgaaaggcg aatacatagt taagatcccg ctggatgtgg ttcggatcgc gatcccgctg
    13201 gtcatctatt ttgtgatcat gttcctggtt tccttcttca tgagcatgaa agtcggggct
    13261 aattatgagc agtcgaccac gttgtcgttt accgcagcaa gcaacaattt tgaacttgct
    13321 atcgcggtgg ctattgctgt atttgg
//
LOCUS       DAOQXS010000012        13095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5252_length_13095_cov_3.701380, whole genome shotgun sequence.
ACCESSION   DAOQXS010000012 DAOQXS010000000
VERSION     DAOQXS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5252_length_13095_cov_3.701380"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKM34_00810"
     CDS             <1..273
                     /locus_tag="PKM34_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82147.1"
                     /translation="PKKINRNNEIEVYPNPARDRVTVGFESTTPAEIVVRIYDLNGKT
                     VKHIDLGTRPQGRQDVIVSCQDIPSGTYIMQVSIGRKVSSAKFIVQ"
     gene            315..2609
                     /locus_tag="PKM34_00815"
     CDS             315..2609
                     /locus_tag="PKM34_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82148.1"
                     /translation="MMKRIFTTLIFLSLVGFLSLSAQTRVYTPELSQPVNGAVDQMPD
                     AVLDWNAVTGGNTGVILYDVQLDDDPAFGSPANFQTELVSAYKTANLGFGQTYYWRVR
                     ARDGNDVSDWSAAWSFRVIRRVVLKSPTDASTQNTDLTLGWNPITGLTQYEYQFDTSY
                     YWNTMSSGITTILYDVATVDETHAWAVGAGGKVLFFDGNSWTAQTSSVTVDLYDVHFL
                     DVNNGWAAGKSGKIIHYDGTQWAAMTTNTSEQVNGITMLSPTSGWAVTKDSAILYYNG
                     ANWAEQYKAAKEMTKIIAVDASHVWAVGKGGVILFYNGSSWTAQQSGTAKDILDAAFV
                     SPDYGWAVGKTGTLIEYKNGTWVPFVNSLTNNRDVNSIKFNSPTSAFAVGKTGLMLQF
                     DGIEWYNQSSGTATTLNGVSMAGTTGFLVGESGVIVSYNDEAFTSPLATIHPVAGDIT
                     TTNLIDLLFGEQYYWRMRAKHSQSTSDWSGVRSFITINTVELDKPPNNTTNADLDIEL
                     KWKKVSNLVTYEIQIDDDVNFGSPIFLASSGIAINAELLKFGTAYNWRVRALHAFDQS
                     DWSAPWKFTTVNAVTLESPANNSTDIKLSPLLTWKKITGIAGYQVMVADNNGFNNPLA
                     TAVVDAAENSFIVPLVLDKDASYFWKVRAINGLDTSNWSPAWTFKTMPPVGIGEPTFE
                     NSIEVFPNPADDIAFIRFNGKGNATLQLTLSDIVGMKVMEKTLNYGEGLRIQSIDVSG
                     LKKGIYLVRLTHGNSAMTRKLIIK"
     gene            2787..3086
                     /locus_tag="PKM34_00820"
     CDS             2787..3086
                     /locus_tag="PKM34_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82149.1"
                     /translation="MKEILPFETPSSLKVSLPTPKDVGSSNLKDSLPTTKAVGSSNLK
                     DSLPTPKAVGSSDLKDSLPTTKAVGSSDIKIKEPTALVVGLGTCHQTVLIIPFPE"
     gene            complement(3063..3818)
                     /locus_tag="PKM34_00825"
     CDS             complement(3063..3818)
                     /locus_tag="PKM34_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82150.1"
                     /translation="MKLFPLIILVGIIALSFHSVYAQEELMEDYDSIAERILLKKEMT
                     GGVTLHTLGMGANFRKGVNKSYFTSRIFEVEVVSMKHPKQVKVIAYYTAKSFVYGKLN
                     HVYMLRAGYGYKKLLNRKPYWGGVELRALYMGGASIAFAKPVYLYFYDLNYQYRYEEK
                     YDPEKPAHSSEFIYGRAPFTDGIWETKIYPGVYAKGALNFEFGKLNTKIRALEVGGVV
                     EFFPIAVPIMAYRPKHNVFATFYLNFSFGKRYN"
     gene            complement(3899..4027)
                     /locus_tag="PKM34_00830"
     CDS             complement(3899..4027)
                     /locus_tag="PKM34_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82151.1"
                     /translation="MKKFIIAIIALGLYALAMTACSSSTDCPAYGEVHKYQIEQRY"
     gene            4144..4833
                     /gene="ung"
                     /locus_tag="PKM34_00835"
     CDS             4144..4833
                     /gene="ung"
                     /locus_tag="PKM34_00835"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ82152.1"
                     /translation="MQTVNPRIEESWKLVLQEEFSAPYFQDLTEFLREEKQKYTVYPP
                     GTQIFSAFDHTPFNRVKVVILGQDPYHGPGQAHGLCFSVPDGVTHPPSLANIFKEIRN
                     DLGFPVPSSGNLTRWAEQGVLLINAILTVRADTPTSHQNKGWELFTDAVIRNLSEKRK
                     NLVFLLWGNYAQAKEKLIDDSRHFILKAAHPSPLSASRGFFGCRHFSETNKILSDLGL
                     KEIDWRLEEQL"
     gene            4830..5420
                     /locus_tag="PKM34_00840"
     CDS             4830..5420
                     /locus_tag="PKM34_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNQ82153.1"
                     /translation="MKELVFATNNAHKLKEIRAIAGDTLRILSLKEIGCTDEIVEDAA
                     TIEGNASLKAFYIWNKYGKYCFADDTGLEVEALDGRPGVKSARYAGDDCNPDNNIKKL
                     LKELLDKPNRKARFRTVISLIADGREVQFEGIVEGEILPEKKGKDGFGYDPVFRPIGY
                     EQSFAEMSPDEKNRISHRGRAVQKLIAHLMRPASFR"
     gene            complement(5383..6372)
                     /locus_tag="PKM34_00845"
     CDS             complement(5383..6372)
                     /locus_tag="PKM34_00845"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNQ82154.1"
                     /translation="MEKKRYIIAIAAGGDSGEYEISLKSAAVVKKHLDPGRMESYLVI
                     IRGNDWKISLEDGTTWPVDRNDFSADLPGRTIKFDGVFIAVHGTPGEDGKLQGYFEML
                     NIPYTSCDSLTSALTFDKFFCNHFAKSFGVNVSRSIVINRGDDIPEKEILTRINLPFF
                     VKPDRGGSSVGTSKVKTPDQLAAALQMAFREDSRVMLEEHIPGRELSCGTITYKGKIM
                     TLPITEIISRKEFFDYEAKYEGMSDEVTPADIPDHVAREISDTSVRLYQLMNCRGMVR
                     FDYILNDTRLFFLEVNTVPGMSELSIVPQQAMKAGISLKDLFTETMLGALNAR"
     gene            complement(6426..7274)
                     /locus_tag="PKM34_00850"
     CDS             complement(6426..7274)
                     /locus_tag="PKM34_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNQ82155.1"
                     /translation="MGTTEQLDTFKIRGDINVDIDIPTTRMSQPNSIAIIIGNRDYRY
                     VSPVEYAINDAESIKDYLVNTLGFKESNILFKPNATKSDFEEYFGNQEYFEAKLYNLV
                     KPGISDVFIYYSGHGAPGLKNHKVYFVPVDCDPSYLEFKGYGLETFYQNLARVPARSF
                     TVVLDACFSGEDIHKNISAVLVKITNPVFSLNNGILLASSASSEPSYWYHDQQHGLFT
                     YFFLRAIKERKISDKDRNDKLTYQEVFDYISSQTDGIPYYSRRLYGKAQNPTLQGPSD
                     RVLVVY"
     gene            complement(7885..8814)
                     /locus_tag="PKM34_00855"
     CDS             complement(7885..8814)
                     /locus_tag="PKM34_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82156.1"
                     /translation="MSFYKSIGSISFRNFIRITPVLLLILNALSVTSQGYTKDVRSYQ
                     SDDKVIIQYRLKVTNKKGYVDYNHRMKSRRFDISLFHSYDQGNQYHSSGNVKGKVGKN
                     IHAGQGKRIIWRPDSEFGYIGNIQFKVEANPRIPKVCWGLSYSRGIYNFGTLRSPEIN
                     RIHLKIYKIGKGNGSTFFPLGISTGNTRVIQDSYIPDSSYILIEIKKTLSKALTVGIG
                     GQTRNRAFSIYVAAGVMIQDEDIEKFKEESDNILVDAGKEFTSDLKFILDGGISIKLF
                     DTKPFTVLLPVNVFWEPIDQDYISINTGLEVQF"
     gene            8915..9931
                     /locus_tag="PKM34_00860"
     CDS             8915..9931
                     /locus_tag="PKM34_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNQ82157.1"
                     /translation="MAINLRKSILTVLTDHSILYKNTIFATLNAITGWIYVIIRQAAL
                     TPAWRNQLTMGALSFLVKKNFYIHFGISVILTIALLLIVISLLKVYTRHGEAYIVPDL
                     TGKEYDRLFEIEETNVFQFIVTDSVFDNSLLPGSVIKQNPSPGSKAKEGRTIYLTIVS
                     YTPKMSRMPELKDLTVRQAVTTLRTGGLKIRKLVFTPHFAGNSVLGQYFEGDTLLAGT
                     EILEGSEIDLLAGLGENRSIRVPIVMGLTRDEARSALQMASLNAGKEHYLDRKDPMHS
                     RVYRQFPSWQEDLYPGDSVTLYFRSDLTYNFDDLLNSVNPDTAVFLDLEVEMPTDSLE
                     PEID"
     gene            9931..12075
                     /locus_tag="PKM34_00865"
     CDS             9931..12075
                     /locus_tag="PKM34_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82158.1"
                     /translation="MKKFFIISFVLSFLSLAIRAQEIITELQENPVVRAEHDRMMANG
                     SRESAGETAPVMLPFFDDFSGDGIYPDSKKWIGEEAFLNTNYGYRMANIGAVTLDAID
                     SRGLLHANASHFPFQADSLTSRPIRLDSIFLPTPRKITVADSIYLSFYYQPQGRAYQA
                     PESTDSLILQFGYSTGDSVFANYYDSIWVQLSDYIHPEDTIFPGDTIFSPAGCADVYV
                     IADNYYYYNDLMRMPCDSVFLPEFRWQRIWSASGTTLAEFYEKYNTYSKQVMIPITDS
                     SRYFKNDFSFRFINFASLASENNPSWRGNCDQWNIDYVYLNINRSYRDTVYRQVSFVE
                     RPPSMLKRYEAMPYNQYTNNPGNEMKNELELVITNLDSTIFNSTYYYVVNRTEGDFQY
                     IYPGGNCNLFPFNLNGYQNCIACAQHACPPVNFVFPLDVTDSAEFTVRHIIIGDITPT
                     DTVSDTIYYAQKFFNYYAYDDGTPEAGYGLNLTPSGAKLAYRFTLNVKDTLRAIQMYF
                     NHTQNDANVKFFDLMVWQDNNGKPGEVIYSQLDEKVEFSSELLGFHTYMLDEPILVNG
                     TFYIGWQQQTPDNLNLGYDRYNNAQQQIFYNTSGEWLQSINTGALMMRPILGKPFDIS
                     SVPDERTVANRITVYPNPLNGTRISFKGDRILPEDYHDLSISVINSLGAEILRMPFSN
                     PTDIGPLCPGLYLIAIKDANGRMVAVTRLIKN"
     gene            12080..>13095
                     /locus_tag="PKM34_00870"
     CDS             12080..>13095
                     /locus_tag="PKM34_00870"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ82159.1"
                     /translation="MPEDLPEIPDSQEGQEESQDLYEHHRFVVDKGQGLLRIDKFLML
                     RIENATRNKIQQAAHAGNILVNGSAVKPNYRVKPQDVISVVLSYPPREIEIIPENIPL
                     KIIYEDEDLIIVDKAPGMVVHPAYGNYSGTLVNALTYHLREVSSDRAPGRTPFLVHRI
                     DKDTSGVMLAAKNELAQARLAAQFFHHTIDRHYTALVWGDFDTDSGTISGHIGRSVRD
                     RKLFTVYIDASQGRHAITHYSVMERFGYVTLVDCKLETGRTHQIRVHFQHIRHPLFGD
                     ETYGGNVILKGTTFPKYRQFVNNCFEILPRQALHARSLSFKHPSTQKIISFESAPPPD
                     MQEV"
BASE COUNT         3603 a         3185 c         2932 g         3375 t
ORIGIN      
        1 ccaaagaaaa tcaaccgcaa caatgagatc gaagtctatc cgaacccggc tcgtgaccgg
       61 gtaacagttg gatttgaaag cactacaccc gcagaaatcg ttgtccgtat ttatgacctg
      121 aacggtaaga cagtcaaaca tatcgatctc gggacaagac cgcagggcag acaggatgtg
      181 atcgtcagtt gccaggatat tccatcggga acatacatca tgcaggtgtc gatcggccgg
      241 aaggtttcat ccgccaaatt tatcgtacaa taactaagct aaaaatttca ttttcaccta
      301 tataaaaaaa taaaatgatg aaaagaatat ttacaaccct gatatttctc agcctggtcg
      361 gattcctcag cctttcggca cagacaaggg tttatacacc ggagctcagt cagccggtca
      421 atggtgcggt tgaccagatg ccggatgcgg tcctcgactg gaacgccgta acgggcggca
      481 ataccggcgt catattatac gatgtgcaac ttgatgatga tcctgcattc ggaagcccgg
      541 ccaattttca gacagaactg gtatcagcat ataaaacagc aaacctggga tttggacaga
      601 cctactactg gagagtacgg gcccgggatg gaaatgacgt ttcagactgg tctgcggcat
      661 ggtcattccg cgtcatccgc cgggttgtac tgaaaagccc gacagatgct tctacgcaaa
      721 atacggacct gacgctgggc tggaacccca taaccggtct tacccagtac gaataccagt
      781 tcgatacgag ttattactgg aatactatga gttccggcat tacaacgatt ctgtatgatg
      841 ttgctacagt agatgaaact catgcctggg ccgtcggagc aggcgggaag gttctattct
      901 tcgacgggaa ctcatggaca gctcagacca gttcggtcac cgttgatctt tacgatgtcc
      961 attttttgga tgttaataat ggatgggctg ccggtaaaag tggcaagatc atccattatg
     1021 acggaacaca atgggcggca atgaccacca atacttcgga acaggtaaac ggcatcacca
     1081 tgctgagccc caccagcggc tgggctgtaa ccaaagacag tgctatccta tactataacg
     1141 gagccaactg ggcggaacaa tataaagccg ctaaagaaat gacaaaaatc atcgccgtcg
     1201 atgccagcca tgtatgggca gtcggaaaag gcggcgtgat attgttctat aacggttcat
     1261 catggactgc acagcagtct ggcacagcaa aagatatcct ggatgccgct tttgtctctc
     1321 cggattacgg ttgggcagtt ggaaaaacgg ggacacttat cgaatataaa aatggcacct
     1381 gggttccttt cgtcaactcc cttaccaaca acagggatgt caattcgatc aaattcaatt
     1441 ctcccaccag tgcatttgca gttgggaaaa cgggtctgat gcttcagttt gacggaatag
     1501 aatggtataa tcaatccagt ggaacggcca caacgctcaa cggagtcagc atggccggaa
     1561 caaccgggtt cctggtagga gagtccggag tgatcgtcag ttacaacgat gaagcattca
     1621 ccagccctct ggctactatt cacccggttg ccggtgatat taccacgaca aaccttattg
     1681 atctcttatt cggcgagcaa tattactggc ggatgagagc caaacactcc cagagcacct
     1741 ccgactggtc aggggttcgc tccttcatta ctatcaatac cgttgaactg gataaaccgc
     1801 caaacaatac gaccaacgcc gatctggata ttgagcttaa atggaagaaa gtctccaatc
     1861 ttgtgacgta tgaaatacag attgatgatg atgtcaactt cggctcaccc atcttccttg
     1921 catcaagcgg aatcgctatt aatgccgaat tgcttaaatt cggcacggct tacaactgga
     1981 gggtcagggc tttgcatgca ttcgatcagt cagactggtc ggcgccctgg aaattcacta
     2041 ctgtaaatgc agttacgctt gaatcacctg ctaacaattc gaccgatatt aagctgtcgc
     2101 cgcttctgac ctggaaaaaa atcaccggaa tagccgggta ccaggttatg gtggccgata
     2161 acaacggatt caataacccg cttgccacag ctgtcgtcga tgcggcggaa aattccttca
     2221 tcgtcccgct ggtactcgat aaagatgcca gctacttctg gaaagtcagg gcgatcaatg
     2281 gcctcgatac ttccaactgg agccctgcct ggaccttcaa gacaatgcct ccggtgggaa
     2341 tcggcgaacc cacttttgaa aacagcatag aagtgtttcc gaacccggca gacgatattg
     2401 ctttcattcg gttcaacggc aaaggaaatg cgacgctcca gcttaccctc tccgacattg
     2461 tcggaatgaa ggtgatggag aaaaccctga actacggaga aggcctcaga atccaatcca
     2521 ttgacgtttc cggattgaag aaaggcattt acctggtccg gctgactcac gggaatagcg
     2581 ctatgacccg aaaactgatc atcaaatagt ccattttgag caatatttaa ggtgagcttc
     2641 ccttccgacc gggtggcaga ttcccttgtg ggtgtctcaa aagtccttcg tgcagcttcg
     2701 tgaaaccttc gtggaacttc gtgtaattta taaataattg ttacacagag atccacgaag
     2761 aaggcacgaa gatacacgaa gttattttga aagaaatctt accttttgag acaccttctt
     2821 cattgaaagt ttcccttccg acgcctaaag acgtcggttc cagcaatctg aaggattccc
     2881 ttccgacgac taaagccgtc ggttccagca atctgaagga ttcccttccg acgcctaaag
     2941 ccgtcggttc cagcgatctg aaggattccc ttccgacgac taaagccgtc ggttccagcg
     3001 atatcaaaat caaggaacca acggctttag tcgttggact gggtacctgt catcaaacgg
     3061 ttctaattat accgtttccc gaatgagaaa ttaaggtaga acgtggcgaa cacattgtgc
     3121 tttggccggt atgccatgat ggggacagct atcgggaaaa attcgacgac tccgccaact
     3181 tccagcgccc tgatcttggt gttcagtttc ccgaattcaa agttcaaagc cccttttgca
     3241 tagacgcccg ggtagatctt cgtttcccat attccatcgg taaacggcgc ccgtccataa
     3301 atgaattcac tcgaatgggc aggcttctcc ggatcgtatt tctcctcgta cctgtactga
     3361 taattcagat cataaaaata aaggtaaaca ggcttggcga atgcaatgga agcgcccccc
     3421 atataaaggg ccctcagttc cacaccaccc cagtaaggtt tcctgttcaa aagcttttta
     3481 tagccatacc cggcccgaag catgtataca tgattgagct ttccgtaaac gaaacttttg
     3541 gctgtgtaat aagcaatgac tttaacctgt ttgggatgtt tcatgctgac gacttcaacc
     3601 tcgaatatcc tggaagtgaa gtaggattta ttgactcctt tcctgaagtt cgcgcccatt
     3661 ccgagagtat gcaaggtaac tcccccggtc atctccttct tcagcaggat ccgctcggcg
     3721 atactgtcgt aatcttccat cagttcctcc tgggcgtaaa cgctgtgaaa ggacaatgcg
     3781 atgataccaa caagtattat cagcggaaag agtttcatag atttaagtaa tgaccaacgc
     3841 atcccattgc aatacgcaat gattatgcca attgacggtc agaaaagaga acaaacgact
     3901 aatacctttg ttcgatctgg tatttatgta cttcgccata tgcagggcaa tccgttgatg
     3961 acgagcaggc agtcatggcc agggcataca accctaaagc aataatggct atgataaatt
     4021 tcttcataat atttgggcga aatattgggt ttgacaaacc tttatcatcg caaagataat
     4081 tttatttatt tttaaaaaca ttaaagaaag aaatatattg cctgaaattt gaaataattt
     4141 gatatgcaaa ctgtaaatcc ccggatcgaa gaaagctgga aacttgtgtt gcaggaggag
     4201 ttttctgccc cctactttca ggatctgaca gaatttctgc gtgaagaaaa acaaaaatac
     4261 acggtatatc cgcccgggac gcagatattc tccgcattcg accacacccc gtttaacagg
     4321 gtaaaagtgg tcatcctggg ccaggatccg taccatggcc ccggccaggc acacgggttg
     4381 tgcttttcgg taccggatgg ggtcacgcat cccccgtcac tggccaatat tttcaaggag
     4441 atcaggaacg acctcggctt tccggtcccg tccagcggaa accttacccg gtgggccgaa
     4501 caaggggttc tgctgatcaa tgcgatcctg acggtaaggg ccgacacccc aacctcacac
     4561 cagaacaagg gatgggaatt atttaccgat gcagtcatcc gcaatctttc ggaaaaaagg
     4621 aagaacctgg tatttctgct ctggggaaat tatgcccagg caaaagaaaa actgatcgac
     4681 gacagccgac atttcattct gaaagcagcc catccatcgc ccttatcggc cagcaggggc
     4741 tttttcggat gccggcattt ttccgaaaca aataaaatcc tgtcggatct cggactgaaa
     4801 gaaatcgact ggagattgga ggaacagcta tgaaagagct tgtctttgcc acgaataatg
     4861 cccataaatt aaaagagatc agggctatag ccggagacac tttgcgaatc ctcagcctga
     4921 aggagatcgg atgcacggat gaaattgtgg aggatgccgc aactattgaa ggtaatgcct
     4981 cattgaaagc attttatatc tggaacaaat acgggaaata ctgttttgcc gacgatacag
     5041 ggctggaagt ggaagccctt gacggcaggc ccggtgtgaa gtcggcccgc tatgccgggg
     5101 atgattgcaa cccggacaac aatatcaaaa agttgctgaa ggagcttttg gataaaccca
     5161 acagaaaagc ccggttcagg acagtcatct cattgattgc cgacggacgg gaggtgcagt
     5221 ttgaagggat tgtcgaagga gagattctgc cggaaaaaaa aggaaaggat ggattcggct
     5281 acgatcctgt tttccggccc attggatatg aacagagctt tgcggagatg agcccggatg
     5341 aaaagaaccg gatcagccac cggggccgtg cggttcagaa actcatcgcg catttaatgc
     5401 gcccagcatc gtttcggtga acagatcctt caggctgata ccggctttca ttgcctgctg
     5461 ggggacaatg ctgagttccg acatacccgg tacggtgttg acttccagga agaaaagcct
     5521 ggtatcgtta agaatataat caaacctgac catcccccgg caattcatca actggtacag
     5581 tctgacagag gtatccgaga tttcacgggc cacatgatcc ggtatatcgg ccggtgtcac
     5641 ttcatccgac atcccttcgt attttgcttc gtaatcgaag aactccttcc ggctgataat
     5701 ctcagtgatg ggcaacgtca tgatcttacc tttgtaagta atagtaccgc acgaaagctc
     5761 cctgccgggg atgtgttctt ccagcatcac gcgggaatct tcacggaaag ccatctgcag
     5821 cgctgccgcc agttgatccg gggtctttac ttttgaagtt cccacactcg atccgccgcg
     5881 atcgggttta acaaaaaacg gcaggttgat ccgcgtcagg atttctttct ccggaatatc
     5941 atctcccctg ttgataacga tcgatctgga tacattgacg ccaaaacttt tcgcgaaatg
     6001 gttacaaaag aacttatcaa atgtcagcgc cgatgtcaac gaatcacagg aagtataagg
     6061 tatgttgagc atttcgaaat atccctggag ttttccgtct tctcccggcg taccgtgcac
     6121 ggcaataaaa acgccgtcga atttaattgt gcgtccggga agatcggctg agaaatcgtt
     6181 cctgtcgaca ggccaggtgg tgccatcctc cagggagatc ttccagtcgt ttccacggat
     6241 gatcaccaaa taactctcca tgcgtccggg atcgaggtgc tttttcacga cggctgcgct
     6301 cttgagcgaa atctcgtatt ccccggaatc acccccggca gcgatggcaa tgatatatcg
     6361 ctttttctcc atagaattaa ataagataac aacagagagt acaaagatat tgaaataccg
     6421 gaaccttaat atacaaccag aaccctgtcg gatgggccct gcagtgtcgg gttttgagcc
     6481 ttcccgtata atcttcttga ataataggga attccatcgg tttgagaaga aatataatca
     6541 aagacttcct ggtaagtcaa cttgtcatta cggtccttat cggatatctt cctttcttta
     6601 attgccctta agaaaaaata ggtaaagagc ccatgctgct gatcgtggta ccaataggac
     6661 ggctcgctgg aagctgatga agccagtaat atgccattat tcagggaaaa aaccggattc
     6721 gtaatcttta ctaaaacagc actgatgttc ttgtgtatat cttcacctga aaagcaggca
     6781 tctaaaacaa cagtaaagct tcgtgcaggg acccgggcca gattttgata aaatgtttcc
     6841 agaccatatc ctttaaattc taaataggaa gggtcgcaat ctaccggaac aaaataaacc
     6901 ttatgatttt ttaggcctgg tgcgccgtgt ccggaataat aaataaagac atctgaaata
     6961 cccggtttca ctaaattgta aagcttagct tcaaaatatt cctgattccc aaagtattcc
     7021 tcgaaatcag atttagtggc attgggttta aatagaatat ttgattcttt aaaacctaaa
     7081 gtgttgacta gataatcctt aatcgattct gcatcattta ttgcatattc caccgggctg
     7141 acatacctgt aatctctgtt accgataata atcgcaatgc tgttgggttg cgacatgcgg
     7201 gtcgtgggaa tatctatatc cacgttgata tccccccgaa ttttaaatgt atccagttgc
     7261 tccgtagttc ccatccgaaa gtcaggggtt ttaaatacta ccggatcaaa ttcgatactg
     7321 attttattct gtttaaagat gagattatcc tggtaattat aatagtatgt ccttttattc
     7381 cccggattat agattgtcgt acgtaataag gcaaagcctg tatcggttaa tgtgtattcc
     7441 ggcgaggaaa atcttaattc atttttattg ctatcgaagt aattagcctg taaattatca
     7501 acgggaactt taagataaac cggcggcaaa ccgaataggg atatcttata cacctcatta
     7561 tccggatcat actccaaaga ggtaatactc aaatgaacca cctccgatgc aatcacatta
     7621 atatgcttga tcgttaattc gtgaatctta ttttcccggt ttttggaatt cactctttcc
     7681 ttgtaatcgg tagtattttc atatctgcct tgcatttgcc actcaccgat ctcctgattg
     7741 actctgtctt caattgttga tttgatcaaa taataatgaa gaatattcag atttgtcgtt
     7801 gtagtttgtg cctctgcacg atttccagat aatccaacaa tcagaacaaa tattatcctt
     7861 aacagcgatt tcataagtca aactttaaaa ttgcacttcg aggccggtat ttatcgaaat
     7921 ataatcctga tctatcggtt cccaaaagac atttacagga agaagaacgg taaatggttt
     7981 ggtatcgaaa agcttaatgc tgattccacc atccagaatg aattttaaat ccgatgtaaa
     8041 ctctttacct gcatccacca gaatattgtc agattcttct ttaaatttct caatatcttc
     8101 atcctggatc ataacaccag cagcaacata tattgaaaat gccctattcc tggtttgtcc
     8161 accaatccca acagtcaatg ctttgcttaa ggttttcttg atttctatta aaatgtaaga
     8221 tgaatcagga atataactat cctgtataac ccttgtgttc ccggtagaaa ttcctaaagg
     8281 aaagaaagta cttccattac cttttccgat tttatatatc ttaagatgaa ttctattgat
     8341 ttccgggctt ctcaatgttc caaaattata aataccccgt gaataggaca gcccccagca
     8401 tactttcgga atcctgggat tagcttccac tttaaactga atatttccaa tatatccaaa
     8461 ttccgaatcc ggtcgccaaa tgatacgctt accctgaccc gcatgaatgt tctttccaac
     8521 tttacctttt acgtttcccg atgaatggta ttggttaccc tggtcatatg aatgaaataa
     8581 gcttatatca aaccttcttg atttcattct gtggttataa tcgacatatc cttttttatt
     8641 ggtcactttt aaccggtact gaataataac tttatcgtcc gattgatagg atctgacatc
     8701 ctttgtataa ccctgggaag taactgataa tgcgttcaga atcaatagaa gaacaggcgt
     8761 gattcttatg aagttccgaa atgaaatgct tccgattgat ttataaaaac tcataatgat
     8821 caaagtttta gctatactat tgtgggttcg cttcggatac taatttgggt ttgctttttt
     8881 ggttaatctt tccaaattta taaaaaaata atttatggcc attaatttga gaaaatcaat
     8941 attaacggtc ttaactgatc attccatcct ttataaaaat actatctttg ccacactcaa
     9001 tgcaataacc ggctggattt acgttattat caggcaggcg gcactgacgc ctgcctggag
     9061 aaatcaattg acaatgggcg ctctgagctt tctggtaaaa aagaattttt acattcattt
     9121 cggcatttcc gtcatcctga caatcgcttt gctgctgatc gttatcagcc tgctgaaagt
     9181 ctatacccgt catggtgaag cgtatatcgt tcccgatctt acgggcaaag aatatgaccg
     9241 gctgtttgaa attgaagaga cgaacgtctt tcagtttatt gtgaccgatt cggtttttga
     9301 caacagcctg ttgcccgggt ctgtcatcaa acagaaccct tcacccggct ccaaagccaa
     9361 ggaaggaagg accatttacc tgaccatcgt ttcgtatacg cctaagatga gccggatgcc
     9421 ggaactgaaa gacctgacgg tcaggcaggc tgtgaccact ttacggaccg gcggcctgaa
     9481 aatccgcaaa ctggttttca caccgcattt tgcgggcaat tcggtgctcg ggcaatattt
     9541 cgaaggcgat acgctgcttg ccggaaccga aatactggaa ggaagcgaga ttgacctgct
     9601 ggccgggctc ggggaaaaca gatccatccg ggtgcctatc gtaatgggac ttacccgtga
     9661 cgaagccagg agcgcactgc aaatggcatc gctcaatgcc ggaaaggaac actacctcga
     9721 ccggaaagac cccatgcaca gccgcgttta caggcagttt ccgtcatggc aggaggacct
     9781 gtacccgggc gattcggtca cactctattt ccgttcggac ctgacttaca atttcgacga
     9841 cctgctgaat tcggtcaatc ccgacacagc tgtctttctc gatcttgagg tagagatgcc
     9901 tacggactca cttgaacctg aaattgactg atgaaaaagt tttttatcat atcttttgtt
     9961 ttatcatttc tcagcctggc tatccgggcg caggagatca ttaccgaatt gcaggaaaat
    10021 ccggtagtac gggctgaaca tgaccggatg atggcaaatg gttcacggga atcggccggg
    10081 gaaacggcgc cggtaatgct cccctttttt gatgatttct ccggcgacgg tatctatccc
    10141 gacagtaaaa aatggatcgg ggaagaggcc tttttaaata cgaactacgg ttaccggatg
    10201 gccaatatcg gggcggttac cctcgacgcc atcgacagcc gggggctcct gcatgccaat
    10261 gcaagccatt ttccgtttca ggccgattca ttaacctcac gccccatcag gctcgactcc
    10321 atcttccttc ccaccccgag gaaaataacc gttgccgatt cgatctacct cagcttctac
    10381 taccagcccc agggaagagc ctaccaggcc cctgaaagca ccgattcgct gatcctccag
    10441 ttcggttatt ccacgggtga ttcggtattt gccaattatt acgattcaat ctgggtgcag
    10501 ctgagcgatt atatccaccc cgaagatacg atttttcccg gcgacacgat attcagtcct
    10561 gccggatgtg ccgatgtcta tgtcatcgcc gataattatt actattataa tgaccttatg
    10621 cggatgccct gcgattcggt ctttcttccc gaattccggt ggcagcgtat ctggagcgct
    10681 tcgggaacga cacttgccga attttacgaa aagtataata cctacagcaa gcaggttatg
    10741 atcccgatca ctgacagcag ccggtatttc aaaaacgact tttctttccg gttcatcaat
    10801 tttgccagcc tggccagcga aaataatccc agctggagag ggaactgcga ccagtggaat
    10861 atcgattacg tgtacctcaa tattaaccgc tcataccgcg atacggtcta ccggcaggtc
    10921 tcctttgtcg aacggccccc cagtatgtta aaaaggtatg aggccatgcc gtataaccaa
    10981 tatacaaata atcccggcaa cgagatgaaa aatgaactgg aactggtgat caccaacctc
    11041 gattccacca ttttcaattc cacatattat tatgttgtga accgcactga aggcgacttt
    11101 caatatatat atcccggcgg aaactgcaac ctcttcccat tcaacctgaa cggataccag
    11161 aattgcatcg cctgcgcaca acatgcctgc ccgccggtca actttgtttt cccgctcgat
    11221 gtcaccgatt ccgctgagtt caccgtcagg cacatcatta tcggggacat cactccgacg
    11281 gataccgttt ccgatactat ttattatgct caaaagtttt tcaattatta tgcgtatgac
    11341 gatggcaccc cggaagccgg ctacggtctt aacctgacgc cttcgggcgc aaagctggca
    11401 taccggttta cactcaatgt aaaagatacc cttcgtgcga tccagatgta ctttaaccat
    11461 acccagaacg atgccaatgt taagttcttc gacctgatgg tctggcagga caataacggc
    11521 aagcctgggg aggtcatcta ttcgcaactc gatgaaaaag tagagttctc ttccgaactg
    11581 cttggttttc atacctatat gctcgacgaa ccgatcctgg taaacggcac cttctacatc
    11641 ggatggcaac agcagacccc cgacaatctg aacctggggt acgaccggta taacaacgcc
    11701 cagcagcaga tcttctataa taccagcggt gaatggcttc aatcgatcaa taccggggct
    11761 ctgatgatga gacccattct gggaaaacct tttgatattt catcggttcc cgatgaaaga
    11821 accgtcgcaa accgtattac ggtttatccg aatccattga acggaacaag gatcagtttc
    11881 aagggagaca ggattttacc ggaagattat catgatcttt ccatttcggt aatcaacagt
    11941 ctgggcgccg aaatcctccg gatgcctttc agcaacccta ccgacatagg gcccttatgt
    12001 ccgggattgt accttatcgc catcaaagac gcgaatggcc ggatggtagc tgtcacacgc
    12061 ctgatcaaga actgagacca tgccggaaga cctgcctgaa ataccggaca gccaggaggg
    12121 acaggaagaa agccaggacc tttacgagca tcaccggttt gtcgtggata aaggccaggg
    12181 gctgctcagg atcgacaaat tcctgatgct ccggattgaa aatgccaccc ggaacaaaat
    12241 tcagcaggca gcgcacgccg gtaatatcct ggtcaatggc agtgcggtca agcccaatta
    12301 ccgtgtcaag ccacaggatg tgatctcagt ggtactctcc tacccgccaa gagaaataga
    12361 gatcatcccc gagaacatcc ctctgaagat tatttatgaa gatgaagacc tgatcattgt
    12421 cgataaagcg ccgggcatgg tggtgcaccc tgcgtatggc aattattcag gcaccctggt
    12481 caacgccctc acctaccatc tcagggaggt ctcgtccgac agggcgccgg gccggacgcc
    12541 cttcctggtg caccggatcg ataaagacac atcgggtgtg atgctggcag ccaagaacga
    12601 gctggcgcag gccaggctgg ccgcacagtt tttccaccat acgatcgaca ggcattacac
    12661 tgcactggtc tggggcgatt tcgacacgga ttcggggacc atttcggggc atatcggccg
    12721 gagcgtgcgc gaccggaagc tcttcacggt ttacatcgac gcgagccagg gccggcatgc
    12781 tatcacccat tattcggtaa tggaacggtt tggctacgtc acgctagtcg actgcaaact
    12841 ggaaacaggc cgcacgcacc agatcagggt tcatttccag catatccgcc atcctttatt
    12901 cggcgatgaa acatacggcg gcaatgtcat cctcaaaggg accacatttc cgaaatacag
    12961 gcagtttgtc aataattgtt ttgaaatcct tcccaggcag gcgcttcatg cccgttccct
    13021 ctctttcaaa catccttcaa cccaaaaaat aatcagcttt gaatcggcac cgcccccgga
    13081 tatgcaggaa gtgat
//
LOCUS       DAOQXS010000013        12877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5365_length_12877_cov_3.968960, whole genome shotgun sequence.
ACCESSION   DAOQXS010000013 DAOQXS010000000
VERSION     DAOQXS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_12877_cov_3.968960"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PKM34_00875"
     CDS             <1..1472
                     /locus_tag="PKM34_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNQ82160.1"
                     /translation="FIESQVKIVLRNCGYIDPEHIEEYEARQGYEAIRKIAKERISRK
                     EVIQVVLDSGIRGRGGGGFPTGLKWKFAHESKNAEKYIICNADEGDPGAFMDRSVLEG
                     DPHSVIEGMIIGAYAIEATGGVIYCRAEYPLAIKRLNIALDQARAKGYLGQNILGIEG
                     FNFDIYIKEGAGAFVCGEETALIASVEGERGMPRKRPPFPAVSGLWRKPTNINNVETY
                     ANIPWIILNGPYAYAKYGTEKSKGTKVFALAGKIKRSGLVEVPMGITIRDIIFKLGGG
                     IQKDRKFKAVQMGGPSGGCIPDYLADTIVDYDSVNATGAIMGSGGMVVMDEDTCMIDV
                     AKFFLDFTQKESCGKCTFCRVGTKRMLEILQRISEGKGEEGDIERLEELAYQIKDNSL
                     CGLGQTAPNPVLTTIRYFRHEYEAHIRDKKCPAKACKSLLTYEVIGEKCTGCTVCAKN
                     CPVNAIDGERKTVHYIRQEDCIKCGMCYTKCKFEAIKLS"
     gene            1637..5359
                     /locus_tag="PKM34_00880"
     CDS             1637..5359
                     /locus_tag="PKM34_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ82161.1"
                     /translation="MSEKVNIILNGNIVEGSRGETILELAKRNGIEIPTLCNDPRLEP
                     FTSCFVCVVEIEGMKGLQPSCSTRLYEGMKITTDNEKIHKARKTALDLLVSNHYADCI
                     GPCKQTCPAGVDVQGYISLIEKGKCHDAVALIKETNPLPAICGRVCVRPCEVACRRNL
                     LDEGAAVGIDYLKRFASDIDIDSPDKWKPELKPATGKKVAVIGAGPAGLSCGFFLQKE
                     GHQVDIFEASPRAGGWLRYGIPEYRLPNDLLQKEVDNITEMGVNIFYNKKFGHDISYK
                     DIKNQYDAMILTIGSQKGTLIGCEGEDAEGVFSGIDFLKEMEMTGQRFNFRGKKVAVI
                     GGGNTAMDCCRTSIRCGADKVYVIYRRTEKEMPANPIEIHESKLEGVEYMFLTLPVKV
                     VKSEAGGVKSITCVKMELGEPDASGRRRPVPVPGSEFDVEIDYILAAIGQKTEADFLD
                     DIKSYATDGEVKANRWGDLEANPDTLQTGIPSVFAAGDGVTGPATLIQAIAQARKASH
                     SCDLYLKGLPVVPITKEFYSSKDVFKPQSNTDYHGLFTERKRHEMPTLDPKDRLNFKE
                     VELGYESEEVAFRETQRCLECGCTALFTCDLKKFSTEYNADQKAQAGEFKEYRVDFRH
                     PYIEIDNNKCILCSRCIRICREVVGAGALGLVNRGFDTYVAPSMGNALYETTCESCGM
                     CISTCPTGAITENVPFKPGPVRLQPAETICNYCSVGCSISLDHKSRFVMHVSGVEGQV
                     NSDGNLCRYPRFGYQYLNDRSRITSPLLKVNGKFEEVTFEKAYEVIRQKIHSTGADDN
                     AFFAGARLSNEELYLVQKLARAGVKTNNVTSFHYLGRGAGYRNNTTAATPFSQIRDAG
                     RVFLIGSEISRDNAVAGFNIYNAQYIKGVKVELVTTKDNSAEAHKADSVLVIKSYYDF
                     IKAINFYLVHNNLHNNLFIRDNCKGFDDYKDELLKESFTDLVQRSGVSAEEIVEFATR
                     YNEEQRAVIVFSEKELSSNACTELQNLSLITGKLGKTGSGLIALKEKNNSQGLHDMGI
                     CRNYGAGGVSLHDAESRSRIQKAWKMADFPAINPVPIVERLESGTLKNLFIFGEDPAG
                     CTFDKAKIHGWLKQAGFIVVQDYFMTETTALADLILPASFPVESGGSFTNTQKVIQEF
                     EAHFTPVSRTTLEQLSDLLKAEGIDQSADYHDVLNEIVSLLPEPGKESFHLFTVTNQD
                     NQARLFEHGCDYLVKRFDSEFTEAFENVKTEVYERV"
     gene            5346..5801
                     /locus_tag="PKM34_00885"
     CDS             5346..5801
                     /locus_tag="PKM34_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ82162.1"
                     /translation="MKEFENIEAILAFAIEQEQKAVDFYTGLAKEAHSEEMKKVFLEF
                     AGEEIRHKKRLTQISEQGLFTMPIEKVADLKIADYTVEVNVSGALSYEQALVLAMKRE
                     KAAFRLYSNLAERAPNKELKDIFLSLAMEESKHKLRFELEYDEFVLREN"
     gene            complement(5960..6457)
                     /locus_tag="PKM34_00890"
     CDS             complement(5960..6457)
                     /locus_tag="PKM34_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNQ82163.1"
                     /translation="MESRPKIKIGQTTTDRFVEIAGWAALAFMWIITLYYFRKLPETI
                     PTHFNMKGTADGFGQKSTIFLLPAIGTVLFIGMTILNRFPHIFNYPVRINSENAIRQY
                     TLATRLLRIFKVSIVLIFLMITWMTSAAANNQGKNSFAWFLPVMLAITFVPLGIYIFK
                     SFRAK"
     gene            complement(6613..7212)
                     /locus_tag="PKM34_00895"
     CDS             complement(6613..7212)
                     /locus_tag="PKM34_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82164.1"
                     /translation="MRLIIILLLILTLPFSPAGQTADEVLSRYFVTELEGNVFMRWTI
                     TAGNTCEDTYIERSADGIAFERIGLIAGICGSPDASITFEFTDTVPVYNQVSYYRLML
                     GYFGYTTPLAIEVKKFNDEGFFAGPNPFRDFTRIAFRNDDNEEYRLLVCDMQGRSVLQ
                     MLTNGNEFIIRREELGSGIYIVKMIKENVTLNHFKLITE"
     gene            7330..10779
                     /locus_tag="PKM34_00900"
     CDS             7330..10779
                     /locus_tag="PKM34_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNQ82165.1"
                     /translation="MNENASGNPANYLQLKAQNIERLKELAAINRTTQIIKEGKSIEE
                     TLKQISTILPPAWQYPQFTAARILYEGKEFASIEFKATPWVQRQRFETIDNKVGYIEV
                     YYLKEFPEIDEGPFMIEERHLIENLANMITGFINGQLAKTIIKPGAGRTAAKSDDGQP
                     VVTSRQLLQRFINKSNEDRDIYHDLMPFKVKEILLVANLYDAYSIEKEGRFSEHVLGE
                     YHTLNLTSIPRITGVSGIDEAMDILGQRHFDLIIMMMGADKRTPIGLSCKVKRLYPYI
                     PVFLLLNNNAEMALISEDKNALEHIDKVFIWNGESSVFFAMIKHVEDKINADNDTDIG
                     MVRVILLVEDSAKYYSRYLPMLYHIVLEQTKRIIEDVSTDELYKILRLRARPKILMVS
                     TYEEATAVMERFRDYLLCLITDVKFNRNGVLDERAGFDLVEYSRSVHKDMPIIIQSSE
                     EENSEMAYRLKCTFINKNSETLSQDFRSFITHFLGFGNFIYRNEQGAKIAEARTLREF
                     ERLLRVIPDESLLYHAKRDHFSLWLTARGEIQAAKILNPQKVTDFENARQIRDYLIDV
                     IGRFRNEQNQGKIIPFEESAITDETNIVSLMDGYLGGKGRGLAFVNTLIYNFDFSKQI
                     PTIKIQAPKTSVIGTEEFEYFMDRNHLRDFVASTTDYEEIKKRFMDAKLTDTLVKRMK
                     VLLTRITKPIAVRSSGLFEDSLMQPFAGIFETYLLPNCHPDPNERLKQALDAVKLVFA
                     SIYSPEARGYIEAVHYKIEEEKMAVVIQEVVGRQYGNYFYPHISGVAQSYNFYPYAHM
                     QPEEGFAVTAVGLGKSVVDGEKAYRFSPKYPTTEILSPKDQSQNTQVEFYAVDLNKKE
                     INLLEGDTAGLAKLDIYEAEMHGNLKHCASVYDADNQVIIPGLTHPGPRLINFANILK
                     YNFVPLAQTIEVVLDVVKEAMGSPVEIEFAVDLTKDNHGRATFYLLQIKPLIGNAMDY
                     NVNIDKIPKEKIILYAEKSMGNGVIDNIEDVIFVDIDTFDKGETMQMTYEIEEMNRKF
                     AAENKKYLLIGPGRWGTRDRWIGIPVNWAQISHARVIIETSLEGYPLDASSGSHFFHN
                     VVSMNVGYFSIQPELSKSYIRWEILKQQPVISQTKYFRHIRFDKPLTVSMDGKKRIAV
                     VTV"
     gene            complement(10852..>12877)
                     /locus_tag="PKM34_00905"
     CDS             complement(10852..>12877)
                     /locus_tag="PKM34_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82166.1"
                     /translation="EQNPVLPVAVDDYARATIFAPVIISPMDNDIPNAADSLILVWPT
                     SDIGYVQRISDSLLSFQPSTASSGQEIVSYYILDKNNYNMVARGRIFVDIEPHHFFDT
                     LNINNIKAWVNADGLLFNKAREIPGMKSYPLLMSYFRYPANQETSTIFNSLIWIGGVS
                     DSGNLHFAGERYRFEGTDFQPGPVSDVYDSTYARKYWRLWKLNREDIEYHRYNWWKSG
                     YQPVDAIVTWPGNGITEAGQADRLAPFKDIGQDGRYDPMEGDYPLIRGDQAIYFIKND
                     DKIHTESDGTRLRLEVHGMVYGFNAPGDSALMHTVFVHYDLINRSENIYYETYAGIWT
                     EIDIGGMWDDYIGSDVGRNTYFGYNGDDFDEDDTEQPEEITKGYGKNPPAQSVTILAG
                     PVIDADGTDNPPGGCDYGVNGINFGNDMVDDERYGLCSFMNNIQLPAHVGNPYYPAPI
                     LYNILKSQWYDGQDLLFGGDGTIYDSRAVGPGCRYVFPGSSDILNLGTGCLPPEEPYN
                     TGGFYWTDSNMNSPGDRRGIGSMGPFTFKPGDVQEIELAYVVANGWNGPVSSVDKLME
                     YIDTLRARVQNGGLIVPNSELGYNEPSKIPGSLKIYPNPASDFVVVEIKGIESGCEYE
                     IYDLFGRMVLSGILSFQAQNVINTNGLSSGLYVLMVKTDDMVYSGKIIRR"
BASE COUNT         3420 a         3187 c         3318 g         2952 t
ORIGIN      
        1 cgttcatcga atcgcaggtg aagatcgttc tgcgcaattg cggttacatc gatccggaac
       61 atatcgaaga atatgaggcg cgccagggtt acgaagctat ccggaagatc gccaaggaaa
      121 ggatctcgcg gaaagaggtg atccaggtcg tcctcgactc gggtatccgg ggccgtggcg
      181 gcgggggatt cccgacagga ctgaaatgga aatttgccca cgagagcaaa aatgctgaaa
      241 aatatatcat ctgtaatgcc gacgagggcg acccgggggc attcatggac cgctcggtac
      301 tggaaggcga cccgcattcg gtcatcgaag ggatgatcat cggggcttat gccatcgaag
      361 ctaccggagg cgtgatctat tgccgcgccg agtacccgct ggccatcaaa cggctcaaca
      421 tcgcactgga ccaggcccgt gccaaagggt acctcggaca gaacatcctc ggaatcgaag
      481 gctttaactt cgatatttac atcaaagagg gcgccggcgc cttcgtatgc ggcgaagaaa
      541 cggccctcat cgcttcggtc gaaggagagc gcggaatgcc gcgcaaacgc ccgccctttc
      601 ctgccgtatc gggcctctgg cgcaagccga ccaatatcaa caacgtggag acctatgcca
      661 acatcccgtg gatcatctta aacgggccct atgcctacgc aaaatacggc acggagaaaa
      721 gcaaggggac gaaagtcttc gccctggccg gcaagatcaa gcgctccggg ctggtggaag
      781 taccgatggg catcacgatc cgcgacatca tcttcaaact cggcggaggt atccagaaag
      841 accgtaaatt caaggccgtc cagatgggag gcccttccgg gggatgtatc ccggactacc
      901 tggccgatac gatcgtcgac tacgactcgg tcaacgctac gggcgccatt atgggctctg
      961 gcggcatggt ggtcatggac gaagatacct gcatgatcga tgtggctaaa ttctttctcg
     1021 acttcaccca gaaagagagc tgcggtaaat gcaccttctg ccgcgtcgga acgaaacgta
     1081 tgctggaaat cctgcagcgt atctccgaag gaaaagggga ggagggtgac atcgagaggc
     1141 tcgaggaact tgcttaccag atcaaggaca actcactttg cggactcggc cagacggcgc
     1201 ctaacccagt gctgaccacg atccgttact tccgccacga gtatgaggcg catatccgcg
     1261 ataaaaaatg cccggcaaag gcatgcaaat cactcctgac ctacgaggtg atcggagaga
     1321 aatgcacagg ctgtacggtc tgcgcgaaga actgcccggt aaatgccatc gacggcgagc
     1381 gcaagacggt ccactatatc cgccaggaag actgtatcaa atgcggcatg tgttatacaa
     1441 aatgtaaatt cgaagcgata aaattatcat aacacccata ttgcatgaat attataacca
     1501 caaaggcaca caaagggatt tcacaaagga acacaaaggt agatgtcaaa gcacctcagt
     1561 acttcgtgcg ccttagtgaa agtcctttga gcgccttcgt ggttaaatag aaatatcaga
     1621 aaggaaaagc aaaactatga gcgaaaaagt caatatcatc ctgaacggca atattgtcga
     1681 gggttcccgc ggagagacca tcctggagct ggccaagcgc aacgggatcg aaatacccac
     1741 cctttgcaac gacccgcgcc tcgagccttt tacctcctgt ttcgtctgcg tggttgagat
     1801 cgagggcatg aaaggcctgc aaccatcatg ttcgacacgg ctgtatgagg gtatgaagat
     1861 cactacggat aatgaaaaga tacataaagc ccgtaaaaca gcgctcgacc tgctggtgag
     1921 caaccactac gccgactgta tcggcccgtg caagcagact tgtcctgccg gcgtggatgt
     1981 ccagggctat atctccctga tcgagaaagg gaagtgccac gatgccgtcg cactgatcaa
     2041 agagaccaac cccctgccgg ccatctgcgg ccgcgtctgc gtccgtccgt gcgaagtggc
     2101 ctgccgccgc aacctgctcg acgagggggc cgccgtggga atcgactacc tgaaacggtt
     2161 cgcctcagat atcgatatcg attcaccgga taaatggaaa ccggaattaa agcccgcaac
     2221 gggaaagaaa gtcgccgtca tcggcgccgg cccggcaggg ctctcctgcg gtttcttcct
     2281 gcaaaaagaa gggcaccagg tggatatatt cgaagccagt cccagggccg gaggttggct
     2341 ccgctacggc atccccgaat accgcctccc taacgacctt ctccagaaag aggtcgataa
     2401 tatcaccgaa atgggggtca atatcttcta taataaaaaa ttcgggcacg atatttcata
     2461 taaagacatt aaaaaccagt atgatgcgat gatcctgacg atcggttcgc agaaagggac
     2521 cctgatcgga tgtgaagggg aagatgcgga gggcgtcttt tccggtatcg atttccttaa
     2581 agagatggaa atgacaggcc agcgctttaa ctttagaggt aagaaggttg cggtgatcgg
     2641 cggcggcaat acggcgatgg actgctgccg tacttccatc cgttgtggcg ccgacaaggt
     2701 atacgtgatc taccggcgca ccgaaaaaga gatgccggcc aacccgatcg aaatccatga
     2761 atcaaagctc gaaggggtgg agtatatgtt tctgacactt cccgtgaaag tcgtgaagag
     2821 tgaagcggga ggcgtgaaga gtattacttg cgtgaaaatg gagctggggg aaccggatgc
     2881 gtcgggaagg agaaggccgg tgccggtgcc gggatcggag ttcgatgtgg agatcgatta
     2941 tatccttgct gctatcggcc agaaaaccga ggccgatttc cttgacgaca ttaagtcata
     3001 tgcgaccgac ggggaggtga aagccaaccg ctggggcgac ctggaagcca atccggatac
     3061 cttgcagaca gggatcccgt cggtttttgc cgccggcgat ggcgttacgg ggccggctac
     3121 cctgatccag gcgatcgcgc aggccaggaa agcatcgcat agctgcgatc tttacctgaa
     3181 agggctgccg gtggtgccta taacgaagga gttctattct tctaaggatg tttttaaacc
     3241 acagagtaac acagattatc acggattatt cacagagagg aaaaggcatg agatgccgac
     3301 actcgatccg aaagaccggc tcaacttcaa agaggttgaa ctgggctatg agtcggagga
     3361 ggtggctttc cgggagaccc aacgatgcct ggaatgcggc tgtaccgccc tttttacctg
     3421 cgacctgaag aaattctcta ccgaatataa tgccgaccag aaagcacagg cgggtgagtt
     3481 taaggagtac cgggtcgatt tccggcatcc ctatattgag atcgataaca acaaatgcat
     3541 cctctgcagc cgctgcatcc ggatctgccg cgaggtagta ggcgccgggg cgctaggcct
     3601 ggtaaaccgc ggtttcgaca cttatgtggc cccgtcgatg ggcaatgctt tgtatgaaac
     3661 cacctgcgaa tcgtgcggga tgtgcatttc tacctgccca accggcgcca ttacagagaa
     3721 tgtgccgttc aaaccgggac cggtccggct ccagccggca gaaaccatct gcaactattg
     3781 ctcggtgggc tgttcgatat cgctcgacca caaaagccgt ttcgttatgc acgtttcagg
     3841 cgttgaaggc caggtgaaca gcgacggcaa cctttgccgg tatcccaggt tcggttacca
     3901 atatctgaac gaccggtcga gaattacctc accgttgctt aaggtgaacg ggaaattcga
     3961 agaggtgacc ttcgaaaaag cttacgaagt catccgtcag aagatccatt ccacaggagc
     4021 cgacgacaat gctttctttg ccggcgcccg gttgtcgaac gaagagctct acctggtcca
     4081 gaaactggcc cgggccggcg ttaagaccaa taacgtgacc agttttcact acctgggccg
     4141 cggggcgggc tatcggaaca acaccacggc ggcaacgccg ttcagccaga tccgcgatgc
     4201 cggcagggtt ttcctcatcg gatcagagat cagccgtgat aacgcggtgg ccggttttaa
     4261 tatctacaat gctcaataca tcaaaggcgt taaggttgaa ctggttacaa caaaagacaa
     4321 tagcgctgag gctcataaag ccgattcggt gcttgttatt aaatcgtatt atgattttat
     4381 caaagccatc aatttttatc ttgttcacaa caatcttcat aataacctct ttatcaggga
     4441 taattgtaaa ggttttgatg attataagga cgaacttctg aaagaaagtt ttacggatct
     4501 ggttcaaaga tccggtgttt ctgccgaaga aatcgtggaa tttgccaccc gttacaacga
     4561 agaacaacgg gcggtcatcg tcttttccga aaaggaattg tcgtcgaatg cctgcacgga
     4621 gctgcaaaac ctgtcgctga tcaccgggaa gctgggcaaa accggcagcg ggctgattgc
     4681 cttgaaggag aagaacaaca gccagggatt gcatgatatg gggatttgcc gcaactatgg
     4741 cgccggcgga gtgtcgctgc atgatgcaga gtcgaggtcc aggattcaga aagcatggaa
     4801 gatggcagac ttcccggcga taaatcctgt tccgatcgtt gaaagactgg agagcggaac
     4861 gttgaagaac ctctttattt tcggcgaaga tccggccggt tgtaccttcg ataaagctaa
     4921 aattcacggc tggctgaaac aggccgggtt cattgtcgtt caggattact tcatgaccga
     4981 aacaaccgcg ctggccgatt tgatcctccc ggcctctttc cccgttgaat cgggcggaag
     5041 cttcaccaac acgcagaagg tgatccagga atttgaggcg catttcaccc cggtctccag
     5101 gactaccctg gagcagttga gtgatcttct gaaggcggaa ggaattgatc agagtgctga
     5161 ctatcatgat gttttgaatg aaattgtctc actgctgccg gaaccgggaa aagaatcttt
     5221 ccatttgttc acggtaacga accaggataa tcaagccaga ttattcgaac acggttgcga
     5281 ttacctggtg aaacgatttg attctgagtt tacagaggca tttgaaaatg ttaaaacaga
     5341 agtttatgaa agagtttgaa aatattgaag caatcctggc ttttgccata gaacaggagc
     5401 aaaaagccgt tgatttctac accgggctgg ctaaggaagc ccattccgaa gagatgaaga
     5461 aagtgttcct ggaatttgcc ggggaagaga tcaggcacaa gaaacggctg acgcagatca
     5521 gcgagcaggg actgttcacc atgcccatag agaaagtggc cgacctgaag atcgccgatt
     5581 acacggtgga ggttaatgtc agcggtgccc tcagctatga gcaggcactg gtgttggcca
     5641 tgaaacggga gaaagccgct ttcagactct attcgaacct ggcggagagg gccccgaaca
     5701 aggaactgaa agatattttc ctgtcgctgg ccatggaaga atcgaaacac aaactgcggt
     5761 ttgaactgga atacgacgag ttcgttttaa gagaaaactg atttagttca aagttgagtc
     5821 agctccgaaa agtgaattta ccctccccta acccctccct actcgtaggg aggggcaggg
     5881 gtgggtactt tggaatatat attacttttc ggagtggact caaattttaa atccggaagt
     5941 ggctaaaact tttcagcggt tatttagccc tgaaggattt aaaaatataa atccccagcg
     6001 gaacaaaggt aattgccagc atcaccggca gaaaccaggc aaaagagttt ttaccctgat
     6061 tgttggccgc agcggaagtc atccaggtta tcattaaaaa gatcagcacg atcgaaacct
     6121 taaatatccg caacagccgg gttgccaggg tatattgtct gatcgcattc tcagaattga
     6181 tcctgaccgg gtaattgaag atatgcggaa agcggttcag aatggtcatg ccgatgaaca
     6241 ggacggttcc gatggctgga agcagaaaga tcgtcgattt ttgtccgaat ccgtccgcag
     6301 tacctttcat attgaaatga gtcgggatgg tttcgggaag tttccggaaa tagtaaagtg
     6361 taatgatcca cataaaagcc agcgcagccc agccggcgat ttctacaaat cggtcggtgg
     6421 tagtttgccc gatctttatt ttcggcctgg attccattca aaataggtac cagcaaagtt
     6481 accaaaagta ttattcgtga tcgtccattt aaatccgtgt cattcgtgtc caattaatgt
     6541 taatggcaca cagatgaaac ggatccgccg gttggcagac aagttgcgac ggatttgcgc
     6601 ggattattac tgttattccg ttatgagctt gaaatgattg agggtcacgt tttctttaat
     6661 catcttaact atatatattc ctgatcccag ctcttccctc cggatgataa attcatttcc
     6721 attggtcaac atttgcagga cagagcgtcc ctgcatatca cagaccagga gccggtattc
     6781 ctcattatcg tcatttctaa aggcaatacg tgtaaaatcg cggaagggat taggacccgc
     6841 aaaaaaacct tcatcattga acttttttac ctcaatagcc agcggtgttg tatatccgaa
     6901 gtaccccagc atcaggcggt aatagctgac ttggttgtag accgggaccg tatcggtgaa
     6961 ctcgaaggta atgctcgcgt ccgggctgcc gcaaatacct gcaatcaggc cgatccgctc
     7021 aaaggcgatc ccgtctgccg accgttcaat ataagtatcc tcacaggtgt tgcctgctgt
     7081 aatggtccac ctcataaaga cattgccttc aagttcggtg acaaaatagc gggaaaggac
     7141 ttcatctgcc gtctggccgg cagggctgaa aggcagagta agtatcagta ataatataat
     7201 aatcagcctc atgtccgcaa atttatctga taaacaattg tcaaaatgaa aagtttaaaa
     7261 caggaagcgg ttttatctcc aaaagcccga tttttcttaa ttttagagca ttattcagac
     7321 tgctgacaca tgaacgaaaa cgcttcgggc aacccggcca actaccttca gctgaaggca
     7381 cagaatatcg aacggttaaa agagctggcc gccatcaacc gcaccaccca gatcatcaaa
     7441 gaggggaaat cgatcgagga gacgctgaaa caaatctcca ccattctccc tcccgcctgg
     7501 cagtacccgc aatttacagc ggcccgtatt ctctacgaag gcaaggagtt tgccagcatc
     7561 gagttcaaag cgactccctg ggtgcagcgg cagcgtttcg agaccatcga caataaagtg
     7621 ggatatatcg aggtgtacta cctgaaggag ttccccgaaa tcgacgaagg gccgttcatg
     7681 atcgaggaac ggcacctgat cgagaacctg gccaacatga tcaccggctt tatcaacggg
     7741 cagctggcaa agaccatcat aaagcccggg gccggacgta ctgccgccaa atccgacgat
     7801 ggccagcctg tagtgaccag ccggcagcta ttgcagcggt ttatcaacaa atccaacgaa
     7861 gaccgggata tttaccatga cctgatgccc ttcaaagtga aagagatcct gctggtcgcc
     7921 aatctttacg acgcttacag catcgagaaa gaaggccggt tttcggaaca tgtcctggga
     7981 gaataccata ccctcaacct gacatcgatt ccccgtatca ccggggtttc cggcatcgat
     8041 gaggcgatgg atatcctggg ccagcgccat ttcgatctga tcatcatgat gatgggggcc
     8101 gacaaacgta ccccgatcgg cctgagctgc aaggtgaaac ggctctatcc ctatatcccc
     8161 gtttttcttc ttctgaacaa caatgccgaa atggctttga tctccgaaga taaaaacgcc
     8221 ctggagcata tcgacaaggt ttttatctgg aacggggaat cgtcggtctt ttttgccatg
     8281 atcaagcatg tggaggataa gatcaatgcc gataacgata ccgacatcgg gatggtgcgt
     8341 gtcatcctcc tggtcgaaga ctcggccaag tattattcgc ggtatctgcc gatgctttat
     8401 catattgtgc tggagcagac caagcggatc attgaagacg taagcaccga cgaactctac
     8461 aaaatcctca ggcttcgcgc caggcccaag atcctgatgg tttcgaccta cgaggaggcc
     8521 acagccgtga tggagcgctt cagggattat cttctgtgcc tcattaccga tgtgaaattc
     8581 aaccggaatg gcgttttgga tgaacgggcc ggcttcgacc tggtggagta ttcccggtcg
     8641 gtacacaagg atatgcccat tatcatccaa agttcggaag aggagaattc ggagatggcc
     8701 tatcgcttga aatgcacctt catcaataaa aattccgaaa cgctgagcca ggatttccgg
     8761 agttttatca cccactttct cggatttggc aattttattt accgtaacga gcagggggct
     8821 aagatcgccg aagcccggac gctgcgggag tttgagaggc tgttgcgggt aattcccgat
     8881 gaatcattgc tctatcatgc caaaagggac catttttccc tatggctcac ggcgcgcgga
     8941 gagatacagg cggcgaagat cctgaacccg cagaaggtca ccgattttga gaatgcccgt
     9001 cagataaggg attatctcat cgatgtgatc ggccggttca ggaacgaaca gaaccagggt
     9061 aagatcatcc cgttcgagga gtcggccatt accgacgaga ccaacattgt aagcctgatg
     9121 gatggttacc ttggcgggaa gggaagaggg ctggcttttg tcaatacgct gatctacaat
     9181 tttgattttt ccaaacaaat cccgaccatc aagatccagg cgccaaagac ttcggttatc
     9241 gggacagagg agttcgagta tttcatggac cggaaccatc tgagggattt tgtggcctcg
     9301 acgaccgatt acgaggagat caaaaagcgg ttcatggatg caaagctgac cgatacgctg
     9361 gtgaagcgga tgaaggtact gctgacacgg atcaccaaac cgattgccgt acgttcgtca
     9421 ggcctcttcg aagattcgct gatgcagcct tttgccggga ttttcgagac ctatctgctt
     9481 cccaactgcc atcccgatcc gaatgagcgg ctgaagcagg cccttgacgc cgtcaagctc
     9541 gtttttgcct cgatctattc gcccgaagcg cggggttata tcgaagcggt gcactataag
     9601 atcgaagagg agaagatggc ggtagtgatc caggaggtgg tcgggaggca atacggcaac
     9661 tatttctacc cgcacatcag cggtgtggcg cagtcgtata atttctatcc ttatgcccac
     9721 atgcagcccg aagagggctt tgccgtgacg gccgtcgggc tgggcaaatc ggtggtcgac
     9781 ggcgagaaag cctaccgttt ttcgcccaaa taccctacaa ccgagatatt atcgcccaaa
     9841 gaccagtcgc agaacaccca ggtggaattt tatgccgtcg atctcaataa gaaagagata
     9901 aatctcctgg aaggggatac tgccgggctg gccaaactcg acatttacga agccgagatg
     9961 catggcaacc tgaagcactg tgcctccgtt tatgatgcgg ataaccaggt cattatcccg
    10021 ggcttgaccc atcccggacc ccggttgatc aattttgcca atatcctgaa atacaacttt
    10081 gttcctttgg ctcagacgat tgaagtggtg ctggatgtcg tcaaggaagc catgggatcg
    10141 ccggtggaga tcgagtttgc tgttgacctg acgaaagaca atcatgggcg ggccaccttt
    10201 tatttgctgc agatcaagcc gctgatcggc aatgcgatgg actataatgt caatatcgac
    10261 aagattccga aagagaagat cattttatat gccgaaaaga gtatgggcaa cggggtgatc
    10321 gataacattg aggatgtgat ctttgtggat atcgacacct tcgacaaggg tgagactatg
    10381 caaatgacct atgagatcga ggagatgaac cggaagtttg ccgcagagaa caagaaatac
    10441 cttttgatcg gtcccggccg gtggggcacg cgcgaccgct ggatcggcat cccggtgaac
    10501 tgggcgcaga tctcgcatgc ccgggtcatc atcgagacca gcctggaagg ttatccgctg
    10561 gatgcttcgt caggttctca tttcttccat aacgttgtgt cgatgaacgt cggttatttc
    10621 agcatccagc ccgagctgtc gaagagctac atccgctggg agatcctgaa acagcagccg
    10681 gtgatcagcc agacgaagta tttccgccat atccgcttcg ataaaccgct gactgtcagc
    10741 atggacggga agaagcggat cgcggtggtg acggtttagc ttagccccca accccagccc
    10801 ttcccgcatc cgggggaagg gggaaattag ttcaattcca tcacagtcgc atcagcgtct
    10861 aatgattttc cccgaataaa ccatatcatc cgttttcacc attaaaacat ataacccgga
    10921 tgataatcca ttggtattga tgacgttttg tgcttgaaat gataatattc ccgatagcac
    10981 catcctgcca aagagatcat agatttcata ttcacaaccg gattctattc cctttatttc
    11041 gacaacaaca aaatccgatg ccggattcgg ataaattttc aggcttcccg ggattttgga
    11101 aggttcattg tatcccagct cgctgtttgg gacaataagt ccgccgtttt gcacccgggc
    11161 acgcagcgta tcgatatatt ccatcagctt atccaccgac gataccggcc cgttccagcc
    11221 gttggcgacg acgtaggcca gttcgatctc ctgcacatcg ccgggtttga aggtgaaagg
    11281 gcccatggag cctattcccc gccggtcgcc gggagagttc atattgctgt cggtccagta
    11341 aaaaccaccg gtattatatg gctcctccgg gggtaaacat ccggtaccga gattcaagat
    11401 atcagatgat ccagggaaaa catacctgca tccgggcccg actgcccgcg aatcgtaaat
    11461 agtcccatct ccgccgaaaa ggagatcctg cccgtcatac cactggcttt taagaatatt
    11521 atataatatg ggagctggat aatacgggtt gccaacatga gcagggagtt gtatgttatt
    11581 cataaagcta cataatccat accgttcatc atcaaccatg tcattgccga aattaatgcc
    11641 gttaacccca taatcacaac caccgggtgg attgtccgtt ccatctgcat cgattacagg
    11701 gccggcaaga atggtgaccg actgtgccgg aggatttttc ccgtaacctt tcgtgatctc
    11761 ttccggttgc tcagtatcat cctcatcaaa atcatcgcca ttgtaaccaa aatacgtatt
    11821 tctaccgaca tcgctcccga tataatcatc ccacattccg ccgatatcta tttcggtcca
    11881 gataccggca taagtttcat aataaatgtt ttccgacctg ttgatcagat cgtaatgaac
    11941 gaacacggta tgcatcaggg cgctgtcgcc aggggcattg aatccgtata ccattccatg
    12001 gacctccaac cttaaccttg taccatccga ttcggtatgg atcttatcgt cattcttaat
    12061 aaaataaatt gcctggtcgc cacggatcaa cgggtaatct ccttccatcg gatcataccg
    12121 cccatcctgg ccgatatctt tgaatggcgc cagccgatca gcctggcctg cttccgtgat
    12181 gccattgccc ggccaggtga cgatagcatc aacaggctga taacctgatt tccaccagtt
    12241 atacctatga tattcaatat cttccctgtt taatttccat aatctccagt actttcttgc
    12301 ataagttgag tcatatacat cggaaaccgg tccgggctgg aaatcggtcc cctcaaatcg
    12361 atatctttct cctgcaaagt gaaggtttcc ggaatcggat actccaccga tccagataag
    12421 gctgttaaag atagtgctgg tctcctgatt agcaggatac ctgaaatacg acatcaataa
    12481 tggataactt ttcattcccg gaatctctcg tgctttatta aacaataggc catcggcatt
    12541 gacccaggct tttatattat tgatgtttaa cgtatcaaaa aaatgatgag gttcgatgtc
    12601 gacaaaaatc cggcctctgg caaccatatt ataattgttc ttatccagga tatagtagct
    12661 aacgatctcc tggcctgaac ttgcagttga aggttgaaaa gagagcaggc tgtcggatat
    12721 ccgctgaaca tatccgatat cggaagtcgg ccataccagg ataagagaat cggcagcatt
    12781 aggtatatcg ttatccatag gtgatatgat aacaggagca aagatcgttg cccgggcata
    12841 atcatcgact gcaactggaa gcacaggatt ttgctcc
//
LOCUS       DAOQXS010000014        12601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5481_length_12601_cov_3.943010, whole genome shotgun sequence.
ACCESSION   DAOQXS010000014 DAOQXS010000000
VERSION     DAOQXS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5481_length_12601_cov_3.943010"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="dnaG"
                     /locus_tag="PKM34_00910"
     CDS             complement(<1..1015)
                     /gene="dnaG"
                     /locus_tag="PKM34_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNQ82167.1"
                     /translation="MISKQTIARILEDARIEEVVGEFVKLKKSGQNYKGLSPFTAERN
                     PSFYVSPAKGIFKCFSSGKGGNVVNFLMEHEHFTYPEALTYLAKKYGIAIEEDSITPE
                     DVQIENERETLFQVTAFAMNFFMQNLHETEAGKAIGLEYFKSRDLKPDTIKKFQLGYS
                     PESWDAFTKNALENGYKLEYLEKTGLTITKDGRSYDRFRGRVIFPIHNITGRILGFGG
                     RTLLKDDHVPKYVNSIESEIYSKSQTLYGLYFAKSAIIRLDNCYLVEGYTDVISMHQA
                     GIENVVASSGTALTEEQIRLIKRYTPNITILYDGDPAGIKASFRGIDMVLEQGMNVRI
                     VLFP"
     gene            1214..1966
                     /locus_tag="PKM34_00915"
     CDS             1214..1966
                     /locus_tag="PKM34_00915"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNQ82168.1"
                     /translation="MTENNLIIPQSQEQLDKTQKARLLMDFFHRIMIHHAMWFAEVQH
                     QYGLDIALGAMKEAYAKSSGIQLNRLAKTLGFEMHDGIPGPLLDLPAEKLDELIESVA
                     VNWLANDGVWFQAVEFKHGLLDAKRCNDTCWAHFSPFEAWSIRNFLNLPENPGLEGLK
                     TALQFRLYATINKQSIVEETPESFVFRMNECRVQNARKRKGLDDYPCKSGGLVEYTTF
                     ARAIDPRIRTECISCPPDPHPGEYYCAWRFSM"
     gene            2166..5531
                     /gene="ileS"
                     /locus_tag="PKM34_00920"
     CDS             2166..5531
                     /gene="ileS"
                     /locus_tag="PKM34_00920"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ82169.1"
                     /translation="MKRKYREYKELNLTRIGKEINAYWKQHATFEKSMHIRDGHREYV
                     FYEGPPSANGKPGIHHVMARAIKDIFCRYKTLKGYLVERKAGWDTHGLPVEIGVEQTL
                     GITKEDIGKKISVEDYNRTCKQEVMKYKDLWDDLTIKMGYWVDLDDPYITFENKYIES
                     VWWLLKKLYEKGLLYEGYTIQPYSPAAGTGLSSHELNLPGAYKEVKDLTVTAQFKVVR
                     NADSEFLFENAFGEVFFLAWTTTPWTLPSNTALAVGKEIVYTRVKSYNPYTNKPITVI
                     LAKDLVNNFFPEKNAELRLEDYQEGQKVIPFRIDGEFKGARLAGMRYEQLLPYARPET
                     GDAFVVVTGDFVTTEDGAGIVHIAPSFGADDFKVARENGIGALTMVDKSGKFVESMGE
                     FAGRYVKPEYDPNFNKGVDESVDVDIVVKLKQENRAFKTEKYVHSYPHCWRTDKPILY
                     YPLDSWFIRTTAFKEQMIELNKTINWKPVSTGIGRFGNWLENLVDWNLSRSRYWGTPL
                     PIWVTEDRSEQKCIGSLEELKGEIGKSVKAGFMKADPLEKFVPGDNSAENYNTFDLHR
                     PYVDEIILVSESGKPMKRETDLIDVWFDSGAMPYAQFHYPFENGQLEHYFPADFIAEG
                     VDQTRGWFFTLHAIAVMLFDSVSFKTCVSNGLVLDKDGNKMSKRLGNAVDPFETLEKF
                     GPDATRWYMMTNSQPWDNLKFDLEGIAEVQRKFFGTLHNTYAFFALYANIDGFHYAEA
                     EVPFEKRPEIDRWILSELNSLIRKVESYYDDYEPTKAGRAIQDFVIDHLSNWYVRLSR
                     RRFWKGDYTEDKIAAYQTLYRCLEVVAQLASPIAPFFMERLFTDLNEVSGRIGSDSVH
                     LTDFPAANESQIDLDLEERMELAQKISSMILSLRKRSNIRVRQPLNKIMIPILNEHNK
                     KQLQAVENLILSEVNVKEIEYLTETAGILVKKIKPNFKTLGPRFGKLMKPIAAAISSL
                     NPEEINKMEEEECLKMTVEGEPIEITIHDVEITTEDIPGWLISSAGNLTVALDINITD
                     ALKKEGLARELINRIQNIRKERDYEVTDKIRVQVVRKDYLDEAIYHNLSYICAEILAE
                     SLVLVEKTEGDNPVEVELTEEFNTIIRVEKINS"
     gene            5565..5972
                     /locus_tag="PKM34_00925"
     CDS             5565..5972
                     /locus_tag="PKM34_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNQ82170.1"
                     /translation="MTDIKKQKADTQEKNRYSDEELEEFKQIILDKLEKANEDLRLLM
                     EAYHLDGEHGTDDTSPTFKILEEGYQVMSKEENSRLASRQQKFINNLENALLRIENKT
                     YGICRVTGKLISKERLKSVPHATLSIEAKLNQR"
     gene            5976..6605
                     /locus_tag="PKM34_00930"
     CDS             5976..6605
                     /locus_tag="PKM34_00930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNQ82171.1"
                     /translation="MKKAILIVFLVLVADQVLKFWIKTHMTIGEEIHVLGNWFILHFT
                     ENEGMAFGITFGGAFGKLILSIFRIIAVIVIAIYMFRMRAKSPHPGLVWSFSLILAGA
                     LGNILDSAFYGLIFSHSSYHTVASLFPPGGGYASFLHGKVVDMLYFPVIDTTLPDWIP
                     IRGGTQFIFFRPVFNIADASITTGVLMLLVFQKKFFPKKEGPVQEVIEN"
     gene            6670..7497
                     /locus_tag="PKM34_00935"
     CDS             6670..7497
                     /locus_tag="PKM34_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNQ82172.1"
                     /translation="MEKKRILFVSQEITPYTEETPIGLKGRYLPQGIQEKGREIRTFM
                     PRYGNINERRNQLHEVIRLSGMNLIIDNNDHPLIIKVASIQQARMQIYFIDNEDYFQR
                     KAQLYDKSGKFFEDNDERAIFFARGVLETVKKLGWAPDIIHCHGWFTGFVPLYLKKSY
                     RDNPLFSETKVVLSIYNDDFKEDFPADISKKIKYEGITKQDLAYYKKTHYVSLLKAAM
                     EYSDGIIAGSPEINTELDSHAKASGKPYLEYQEEDKYIDAFSDFYDKVLNEETGKAK"
     gene            7537..8865
                     /locus_tag="PKM34_00940"
     CDS             7537..8865
                     /locus_tag="PKM34_00940"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HNQ82173.1"
                     /translation="MIKKLSWLFAGIILVMVFNMAGCKQDDNDLGIDIQPPNDKLNVF
                     SSDTATILAYSTLVDSVKTDETSVTLLGSLVDPVFGRSTASFYTQLRLSKTAYDFGST
                     PYPDSIVLTLDYDDIYGDTLAPMTIRVYEMSERIFIDSMYYSNHSVAYESDLLAEKTF
                     TPDLSTDVIIGEDTLDPHLRINLGQFSFSLAEKLMNAPADSMASNASFINYFYGLYVT
                     AEPATAGGSIVYFNLLSELSEMTLYYHNGEDDSLRFSYLINSNCARFSHFDHDYSLGS
                     PEFKAQVIDGDTTLGKSVCYVQALGGVKTYIRFPNIKNYYNNGKIAVNEARFFLQAYE
                     QNPELARAASLIMVKRNGDGSYSILNDQLDGDSYFGGFYDESKGGYWFRITSTVQELM
                     RSDSTDYGYEIYLSGGSINSERVLLSGTNPQSPVDAEDRMKLVITYTTLN"
     gene            8876..10714
                     /gene="glmS"
                     /locus_tag="PKM34_00945"
     CDS             8876..10714
                     /gene="glmS"
                     /locus_tag="PKM34_00945"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNQ82174.1"
                     /translation="MCGIVAYLGPQEAYPILMKGLYRLEYRGYDSAGIAILNDELSVL
                     KTKGKVADLETLTKNMALEGTIGIAHTRWATHGEPNDINAHPHYSQSKNLAIIHNGII
                     ENYLTLKKELINRGYKFTSNTDTEALIHLIEDIQLNEKVDLVEAVRIALNQVVGAYAI
                     VIISKNHPDMLIAARKSSPLVVGIGAEELFVASDATPIVEYTKDVVYLNDEELAILRR
                     GKPLKIITIKNKEKTPYIQKLELNLSALEKSGFEHFMLKEIFEQPRSIRDSLRGRLRL
                     QEGTVSLGGIVDYEQKLMNAERIIIVACGTSWHAGLVGEYLLEELARIPVEVEYASEF
                     RYRNPILCEKDVVIAISQSGETADTLAAIELAKGRGATILGICNVVGSSIARATHAGS
                     YTHAGPEIGVASTKAFTAQVAVLAMMALTIASKKGTIPKSRFLRIIHELDLIPEKVEK
                     TLQVDPLVRSIAEMYKDHRNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDEEMPVVFVATNKGTYEKVLSNIQEVKARKGKIIAIVTEHDEQVREMADHVI
                     EIPETEEMLVPLLATVPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(10807..11184)
                     /locus_tag="PKM34_00950"
     CDS             complement(10807..11184)
                     /locus_tag="PKM34_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ82175.1"
                     /translation="MKIHNHEDLEVFQQSFSAAVEIFKLTKTFPGEELFSLTDQIRRS
                     SRSVCANISEAFRKRYYPKSFTAKITDAEGEAAETQVWLKFCLHFSYIEQIKYNELME
                     AYNNILGKLVVMRNNPNKWSIDH"
     gene            complement(11392..11967)
                     /locus_tag="PKM34_00955"
     CDS             complement(11392..11967)
                     /locus_tag="PKM34_00955"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNQ82176.1"
                     /translation="MNHELPSLPFALDALEPFISKNTLEFHYGKHHQAYVTNLNKLLP
                     GSPFENATLEEIVMKATGGIFNNGAQVWNHTFYWHCLSPFGGGMPAGKLMEMIDRDFG
                     SYEGFKEHFTQAAATLFGAGWAWLSIDGYGKLVITQESNAGNPMRNGLKPLLTCDVWE
                     HAYYLDKQNRRPDYIGDFWKIVDWKAAEGRV"
     gene            complement(12109..>12601)
                     /locus_tag="PKM34_00960"
     CDS             complement(12109..>12601)
                     /locus_tag="PKM34_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82177.1"
                     /translation="DRYANGEMENPDSISLTDTVKYYTPAGKVVYGGGGIMPDVYVAL
                     ATEEKEVLYRKLIHQNTIFQYAFNYTDRERPSLAKYSTPKEFIERFKVDDKLFSDLMD
                     YARNQDIYYTPSELIDSKEKIKTLLKAYIGRNLLDDEAFYPVYHEVDKVFQIALDTLI
                     KNN"
BASE COUNT         3373 a         3209 c         3051 g         2968 t
ORIGIN      
        1 ccggaaataa aacgatcctg acattcattc cctgctccag caccatgtcg atcccccgga
       61 atgaagcctt gatccccgcc ggatcaccgt cgtacaaaat cgtgatattc ggcgtgtaac
      121 gcttgatcag ccgtatctgc tcctccgtga gcgccgtgcc cgatgaagcc accacgtttt
      181 caatcccggc ctggtgcatc gaaatcacat cggtataacc ctccaccagg taacagttgt
      241 cgagccggat aatggcgctc ttcgcaaaat aaaggccgta aagggtctgg ctcttgctgt
      301 atatctccga ttcgatcgaa ttgacatact tcgggacgtg gtcgtccttc agcaaggtcc
      361 gcccgccaaa acccagaatc cgaccggtta tgttgtggat cgggaaaatg acccggcccc
      421 tgaaacggtc ataactccgg ccgtctttcg tgatcgtcag ccctgtcttt tccaggtact
      481 ccagtttgta gccgttctcc agggcgttct tcgtgaatgc atcccagctc tctggactgt
      541 aacccagctg gaattttttg atcgtgtcgg gcttcaggtc gcggctcttg aaatattcaa
      601 gcccgatggc tttgccggct tccgtctcat gcaggttttg catgaagaaa ttcatggcaa
      661 acgccgtcac ctggaaaagc gtttcccgtt cattttcaat ctgaacatct tcaggagtaa
      721 tcgaatcctc ctcgatggct atgccgtact ttttcgccag gtaggtcagt gcttcgggat
      781 aggtgaaatg ttcgtgttcc atcaggaaat tcaccacatt tcccccctta cccgaactga
      841 aacatttgaa gatccccttg gccggcgaca catagaacga agggttgcgt tcggccgtaa
      901 acggactcag ccccttgtaa ttctgcccgc tttttttgag tttgacaaat tcgccgacaa
      961 cctcttcaat acgggcgtct tccagaatac gcgctatggt ttgtttggag atcaaggtta
     1021 ttaatatgct ttacaaagat aataatgcat tagagggaag gggaaattgt tgataatatc
     1081 ttgagatggc atctcaaatc gatcgtccaa tcttctgttt cgcttgccag ataaaccatt
     1141 cctgatttta atcagatatt ctgagatgcc atctcaaaaa acaataaatt agccccttaa
     1201 ttataaccta cccatgacag aaaacaatct cataatcccc caatcgcagg aacagcttga
     1261 caaaacacag aaagcccgcc tgctgatgga ctttttccac cgtatcatga tccaccatgc
     1321 catgtggttt gccgaagtgc agcaccaata cggtctggat atagccctcg gtgccatgaa
     1381 agaggcgtat gccaaaagtt ccggcatcca gctgaaccgg ctggcgaaaa cgctgggatt
     1441 tgagatgcat gacggaattc ccggcccgct cctcgacttg cccgctgaaa aactcgacga
     1501 actgatcgaa agcgtggcgg tcaactggct ggccaacgac ggtgtctggt tccaggcggt
     1561 cgagttcaaa cacggcctgc tcgacgccaa gcgctgcaac gatacctgct gggcacactt
     1621 ctcgccgttc gaagcctggt cgatccgtaa tttcctcaat ctgccggaaa atccgggcct
     1681 ggaagggctt aagaccgcgc ttcaattcag gctatatgcc accatcaata aacagtcaat
     1741 cgtcgaagaa acccccgaaa gtttcgtctt ccggatgaac gaatgccgcg ttcagaatgc
     1801 ccgtaaacgg aaagggctgg atgattatcc ctgcaaatca gggggcctcg ttgaatacac
     1861 cactttcgcc cgggccatcg acccgaggat caggaccgaa tgcatctcct gcccgccgga
     1921 tccgcacccc ggggagtatt actgcgcgtg gaggtttagt atgtagtcac tggtcatgag
     1981 ctcgcttcgc tctcgtcatt gagggtcata aagttgtgtt aaattagtca taaggtcgtc
     2041 ataaggtggt ttaaggttta ttgattgaga tttgacattt gagttttgtt tgggtttcgg
     2101 aatttggaat ttggaatttc cgtacctttg cgcctcattt ttaatttttt gcaactaaag
     2161 gctccatgaa acgcaaatac agggaataca aggaactgaa cctgacccgc atcggcaagg
     2221 agatcaatgc gtactggaaa cagcacgcga cttttgagaa aagcatgcac atcagggacg
     2281 gacaccggga gtatgtattt tacgaagggc cgccgtcggc taacggaaaa ccaggtatcc
     2341 accacgtgat ggcccgggca atcaaagaca tcttctgccg ctataaaacc ctgaaagggt
     2401 acctcgtcga gagaaaagca ggctgggata cgcatggact tccggttgaa ataggcgtgg
     2461 aacagacgct gggaattacc aaagaagata tcggaaagaa aatttccgtt gaagattata
     2521 accgtacctg caagcaggag gtgatgaaat acaaggatct atgggacgac ctgaccatta
     2581 aaatggggta ctgggtcgac ctggatgatc cgtatatcac gtttgaaaac aaatatatcg
     2641 aatcggtctg gtggctgctt aagaaactat acgaaaaggg gcttctttac gagggataca
     2701 cgatccagcc ttattcgccg gcagcaggaa cagggctcag ctcgcatgaa ctgaacctcc
     2761 cgggtgccta caaggaggtc aaagacctta cggttaccgc ccagttcaag gttgtcagaa
     2821 atgcagactc ggaattcctt ttcgaaaatg cctttggcga agtgtttttc ctggcctgga
     2881 caacgacccc atggacgctt ccgtcgaata ccgcactggc tgtcggaaaa gaaatcgttt
     2941 acaccagggt gaaatcctat aatccttata cgaataaacc catcactgtc atcctggcca
     3001 aagacctggt aaacaacttc tttccggaga aaaatgcaga gctgagactg gaagattacc
     3061 aggaagggca aaaggtcatc cctttccgga tcgatggaga atttaaagga gcccggctgg
     3121 ccggtatgcg ctatgagcaa cttcttccct atgcccggcc cgagacaggc gatgccttcg
     3181 ttgtggtgac cggtgatttt gtgactacgg aagacggcgc cggcatcgtg catatcgccc
     3241 cgagctttgg cgccgacgac ttcaaagtgg ccagggaaaa cggtatcggc gcactgacga
     3301 tggtcgacaa atcgggaaaa ttcgtcgaat cgatgggcga attcgccggc cgctatgtca
     3361 aacccgaata cgacccgaac ttcaacaaag gggtcgatga aagcgtggat gtcgatatcg
     3421 tggtgaaact caagcaggaa aacagggcct tcaaaaccga gaagtacgtt cacagctatc
     3481 cgcattgctg gcgcaccgat aaaccgatcc tctattatcc gctcgactcg tggtttatcc
     3541 ggaccacagc ctttaaagag cagatgatcg agctgaacaa aacgatcaac tggaaaccgg
     3601 tgtcgaccgg tatcggccgg ttcggcaact ggctcgaaaa cctggtcgac tggaaccttt
     3661 cccgttcccg ctactgggga acgccgctgc cgatctgggt gacggaagac cgcagcgagc
     3721 agaagtgcat cggctcgttg gaggaattga aaggggaaat tggaaaatcg gtaaaggcag
     3781 ggttcatgaa agccgatccg ctggagaagt tcgttcccgg cgacaattcg gccgaaaatt
     3841 acaacacctt cgacctgcac cggccttacg tcgatgagat catcctggta tcggaatcgg
     3901 gtaaaccaat gaagagggaa accgacctga tcgacgtgtg gttcgattcg ggcgccatgc
     3961 cgtatgccca gttccactat ccgtttgaga acgggcagct ggagcactat ttcccggccg
     4021 actttatcgc cgaaggagtc gaccagaccc ggggttggtt ctttacgctt catgccattg
     4081 ccgtcatgct tttcgactcc gtatccttca aaacctgcgt ctccaacggc ctggtgctcg
     4141 acaaggatgg caacaagatg tcgaaacggc tgggcaatgc cgttgatccc tttgaaacgc
     4201 tggaaaaatt cggccccgat gccacgagat ggtacatgat gaccaactca cagccgtggg
     4261 acaatttgaa gttcgacctg gaggggatcg ctgaggtgca gcgcaaattt ttcggcaccc
     4321 tccacaacac atacgcattc ttcgcccttt atgccaacat cgacggattc cattatgctg
     4381 aggcagaggt gccgtttgaa aagcggccgg agatcgaccg gtggatcctt tcggagctta
     4441 actccctgat ccgcaaagta gagagctatt acgacgacta tgagccgacc aaggccggcc
     4501 gtgccataca ggattttgtg atcgaccacc tgagcaactg gtatgtgcgt ttgtcgaggc
     4561 gccggttctg gaaaggcgat tataccgaag acaagattgc ggcataccag acgctttacc
     4621 gctgcctgga ggtagtggca cagctcgcct cgcccatcgc ccccttcttc atggagcgcc
     4681 tctttaccga tctgaacgag gtgtcgggga ggatcgggtc cgacagcgtt cacctgaccg
     4741 attttcctgc ggccaatgag agccagatcg accttgacct tgaagaacgg atggaactgg
     4801 cccagaaaat ctcttccatg atcctgtcgc ttcgcaagcg ttcgaatatc cgcgtccgcc
     4861 agccgctgaa caagatcatg atcccgatcc tgaacgagca caataaaaag cagcttcagg
     4921 ctgtggaaaa cctgatctta tcggaagtga atgtcaaaga gatagaatac ctgaccgaaa
     4981 cagccggcat tctcgttaag aaaatcaagc cgaatttcaa aactttagga ccacggttcg
     5041 ggaaactgat gaaaccgatc gccgcagcca tctcctcact caacccggaa gagatcaata
     5101 aaatggaaga ggaagagtgc ctgaagatga cggtagaagg agaaccgatc gagatcacga
     5161 tccatgacgt cgaaatcact acagaagaca tcccgggctg gctgatctcc tcggccggca
     5221 acctgacggt agccctggat atcaacatca ccgatgcgct gaaaaaggaa ggattggcca
     5281 gggagctgat caaccgcatc cagaacatcc ggaaggagcg tgattacgaa gtgaccgaca
     5341 agatcagggt gcaggtagtg cgtaaggatt atctcgacga ggccatatat cacaatttat
     5401 cttatatttg cgccgaaatt ctagccgaat cacttgtttt ggttgagaaa accgaaggag
     5461 ataacccggt ggaagttgaa ctgaccgaag agttcaacac aatcatcagg gtggaaaaaa
     5521 ttaattcata atcaataatt ttttaacgga ttaaatcccg ggccatgaca gacattaaga
     5581 aacagaaagc cgatactcag gaaaagaaca ggtattctga cgaagaactg gaggaattca
     5641 aacagatcat cctggataaa ctggaaaagg ccaacgaaga cctcaggttg ctaatggagg
     5701 cgtatcatct tgacggagaa catggtacag atgatacatc tcctactttc aagatcctgg
     5761 aagaaggata ccaggtcatg tcgaaagaag agaacagccg gctggcctca cgccagcaaa
     5821 agtttatcaa caacctggaa aatgccttgc ttcgcatcga gaacaagacc tacggcatct
     5881 gcagggtgac aggcaagctc atttcgaaag aacggctcaa gagcgtaccg cacgccacac
     5941 tcagcatcga agccaagctt aaccagagat aggccttgaa gaaagcaatc ctgattgttt
     6001 ttctggtgct ggtcgccgac caggtgctca aattctggat caaaacccat atgaccatcg
     6061 gggaagagat ccatgtgctg ggcaattggt ttattcttca ttttaccgaa aatgagggga
     6121 tggcattcgg catcacattc ggaggcgcct tcgggaaact gatcctgagc atattccgga
     6181 tcattgccgt tatcgtgatc gccatttaca tgttccggat gcgggccaaa tcgccccatc
     6241 ccgggctggt ttggagcttc tcgctcatcc tggccggtgc attgggaaac atcctcgaca
     6301 gcgcttttta cggactgatc ttcagccata gcagttatca tacagtagct tcgcttttcc
     6361 cacccggcgg aggatacgcg tcgttccttc acggaaaagt ggtcgatatg ctctattttc
     6421 ccgtcatcga taccacattg cccgattgga tcccgatccg tggggggacg caatttattt
     6481 ttttccgccc cgttttcaac atcgccgacg catccattac caccggcgtt ttaatgctgc
     6541 tggttttcca gaagaaattc tttcccaaaa aagaaggtcc agtgcaagag gtaattgaaa
     6601 attgattaat tacttaattt taactacctt tgcacttctt tttttagaaa cacacacaag
     6661 cttccggtta tggaaaaaaa gaggatcctc ttcgtatcgc aggaaatcac cccatatact
     6721 gaagaaacac ccatagggct caagggccgc taccttccac aggggataca ggaaaaaggc
     6781 agggaaatcc gcaccttcat gccccgttac ggcaacatca atgaacgccg aaaccagctt
     6841 cacgaagtga tccgcctttc gggcatgaac ctcatcatcg acaacaacga ccacccgctc
     6901 atcatcaaag tcgcctccat ccagcaagcc cggatgcaga tctattttat cgacaatgag
     6961 gactacttcc agcggaaagc ccagctttac gataaatcgg ggaagttttt cgaggataat
     7021 gatgaacggg ccatcttttt cgcgcgcggc gtgctcgaaa cggtaaaaaa gctcgggtgg
     7081 gcccccgata tcatccactg ccacggctgg tttaccggat ttgtccctct ataccttaaa
     7141 aaatcgtacc gcgacaatcc ccttttcagt gaaactaaag ttgtcctatc tatttataat
     7201 gatgacttta aggaagattt tccggccgat atctccaaaa aaattaaata tgaaggtata
     7261 accaaacaag accttgcata ctacaagaag acccattacg tttcattact caaggcggca
     7321 atggaatatt ccgacggtat cattgccggg agccctgaaa ttaacacaga actggattca
     7381 catgccaaag cttccggaaa accttacctg gaataccagg aagaagataa atatatcgat
     7441 gctttctcag atttttacga caaagtactg aacgaagaga ctggaaaagc aaagtaactt
     7501 atcgacgcct catcatttta caaacaaacc gcaagaatga taaaaaagct gagttggtta
     7561 tttgcaggga taatcctggt tatggttttc aacatggccg gttgtaaaca ggatgataat
     7621 gacctgggga ttgatatcca gcctcctaac gacaagctga acgtattttc atccgacacg
     7681 gctaccattt tagcctattc gactctggtc gattcggtga aaaccgacga aacatccgtt
     7741 acactcctcg gcagcctggt cgatccggta ttcggacgct ccacggccag tttctacaca
     7801 cagctcaggc taagcaagac ggcctacgat tttggctcta ccccttatcc cgactctatt
     7861 gtccttaccc tcgattacga tgatatttac ggagatacac tggctccgat gaccatccgg
     7921 gtttatgaaa tgtcggagag gatctttatc gactccatgt actattcgaa ccattctgta
     7981 gcttacgaga gcgacctcct ggccgaaaag acctttaccc ctgacctatc caccgatgtg
     8041 atcatcggtg aagacacact ggatccgcac ctgaggatca acctggggca gttctcattt
     8101 tcacttgcag aaaaactgat gaatgccccg gccgacagca tggccagtaa cgccagcttc
     8161 atcaactatt tctatggact gtacgtcacg gccgaaccgg caaccgccgg aggttccatc
     8221 gtctatttca acctgttgtc agaactttcc gaaatgacgc tgtattatca caacggggaa
     8281 gatgattcac tccggttttc ctacctgatc aacagcaact gtgcccggtt cagccatttc
     8341 gatcatgatt attcgctggg gagccctgaa tttaaagcgc aggtgatcga cggcgatacc
     8401 accctgggaa aatcggtttg ttatgttcag gctttaggtg gcgttaaaac atatatccgt
     8461 tttccaaata ttaaaaatta ttataacaac ggaaagatag cggtcaatga ggcccggttt
     8521 ttcctccagg cctatgaaca aaaccctgaa ctggcccgtg ccgcttcgct gatcatggtg
     8581 aaacgaaacg gcgacggttc ctatagtatt ttaaacgacc agcttgacgg cgacagctat
     8641 tttggcggat tttacgatga atccaaaggc ggatactggt tccggatcac atcgaccgtc
     8701 caggaactga tgcggagcga cagtaccgat tacggctacg aaatatacct gagcggcgga
     8761 tcgatcaatt cggaacgtgt gttgctaagc ggaacaaatc cccagtctcc ggttgatgcc
     8821 gaggaccgta tgaagctggt cattacctat acaaccttaa actgaaaacc aatctatgtg
     8881 cggaattgtt gcttaccttg gcccacagga agcttacccg atcctgatga aaggcctgta
     8941 ccggctcgaa tacaggggct acgacagcgc cgggatcgcc attcttaacg atgaacttag
     9001 cgttctgaaa accaaaggaa aagtcgctga tcttgagacc ctgacaaaaa atatggcgtt
     9061 ggaaggaacg atcggaatag cccacacacg atgggcaacc catggcgaac ccaatgatat
     9121 caacgcccat ccccattatt cccaatccaa aaacctggcg atcatccata acggtatcat
     9181 cgaaaattat cttaccctga aaaaagagct gatcaaccgg ggatataagt ttaccagcaa
     9241 taccgacacc gaagccctca ttcacctgat cgaagacatt cagctcaacg aaaaggtcga
     9301 cctggtcgaa gcggtgcgga ttgccctgaa ccaggttgtg ggcgcttatg ccatagtgat
     9361 catctcaaag aatcaccccg acatgctgat cgccgcccgc aaaagttcgc cgctggtggt
     9421 cgggatcggg gcagaggaac tcttcgtcgc atccgacgca accccaatcg tagagtacac
     9481 aaaagacgtc gtttacctga acgacgagga actggccatt ctccgcagag gaaagccgct
     9541 gaagatcatc actataaaga acaaagaaaa gactccttac attcagaaac tcgagctgaa
     9601 tctttctgcc ctggagaaaa gcggttttga gcatttcatg ctgaaagaga tattcgagca
     9661 gccccgctcc atccgcgaca gtctgcgcgg caggctcagg ttgcaggaag ggaccgtgtc
     9721 actcggcggg attgtcgact atgagcaaaa actgatgaat gcggaacgga tcatcatcgt
     9781 agcctgcggg acatcctggc atgccggtct tgtcggcgaa tacctgctcg aagagttggc
     9841 caggataccc gttgaagtcg aatatgcatc cgaatttcgt taccgcaatc ctatcctgtg
     9901 cgaaaaagat gttgtgatcg ccatctcgca atcaggtgaa acggcggata cgctggccgc
     9961 cattgagctg gccaaaggga gaggcgccac gatcctggga atctgtaatg tggtcggttc
    10021 ctcgatcgcc cgggctaccc atgcgggctc ctacacccat gctggcccgg agatcggcgt
    10081 agcctccacc aaagcattca cggcacaggt agccgtactg gccatgatgg cgctgaccat
    10141 tgcctcgaaa aaaggaacga tcccgaaatc ccgctttctc cgtatcatac acgaactcga
    10201 cctgatcccg gaaaaagtgg agaagaccct acaggtcgac ccgctcgtcc gttcgattgc
    10261 agagatgtac aaagaccacc gcaacttcct ttacctgggg agaggctata atttcccggt
    10321 ggcgctggaa ggggccctga agctgaaaga gatttcctac attcatgccg aaggctatcc
    10381 tgccgccgaa atgaaacatg gccctatcgc cctgatcgac gaggagatgc cggtggtatt
    10441 cgtggccacc aacaaaggca cttacgaaaa agtgctcagc aatatccagg aggtgaaagc
    10501 ccgcaaagga aaaataattg ccattgtaac cgaacacgat gaacaggtcc gtgagatggc
    10561 cgaccatgta atcgagattc cggagacgga ggagatgctg gtaccgttgc tggcgacggt
    10621 accgctgcaa ttattgtcct accatatcgc cgtgatgaga ggctgcaacg tcgatcagcc
    10681 gaggaatctg gcgaagagcg taacggtcga atagggagac aaggagatgg ggagacaagg
    10741 agatggggag acaaggagac ggggagacaa ggagatgggg agattaggag acggagagag
    10801 gatgtgttaa tgatcgattg accatttatt aggattattc ctcattacga ccagctttcc
    10861 aagaatattg ttgtaagctt ccatcagttc attgtattta atctgttcaa tatagctgaa
    10921 atgtaagcag aatttcaacc aaacctgcgt ttcggcagct tctccttctg catcggtaat
    10981 tttagcagta aaggattttg gataatacct ttttctgaag gcttcagaga tatttgcaca
    11041 aactgaccgg gacgaccgtc tgatttgatc agtaagtgaa aacagttctt ctcccgggaa
    11101 ggtctttgtc aatttaaaaa tctcgacagc ggctgaaaac gattgctgga aaacttccag
    11161 atcttcatga ttgtgaatct tcataaacaa gaaattttaa ttatacattt ttcttgttta
    11221 tccggaaaat caacagaaaa tacccggtaa ccttaaaata tttattaaca aaacatctct
    11281 ccccgtctcc ttgtctcccc gtctccttgt ctccttgtct ccccatctcc ttgtctcccc
    11341 atctccttgt ctccccgtct ccttgtctcc ccgtctcctt gtctccctgt cctagactct
    11401 cccctccgcc gccttccagt ccacaatctt ccagaaatcg ccaatataat cgggccggcg
    11461 gttctgctta tcgagataat aggcgtgctc ccagacgtcg caggtgagaa ggggcttcag
    11521 gccattacgc atcgggttgc cggcattgct ttcctgcgtg atgaccagtt ttccgtagcc
    11581 gtcgatcgac agccaggccc agcccgcgcc gaacagggtg gcagccgcct gggtgaagtg
    11641 ctctttgaaa ccttcatacg acccgaagtc gcggtcgatc atttccatca gttttccggc
    11701 cggcatgcct cctccgaagg ggctcaggca gtgccagtag aatgtatggt tccacacctg
    11761 ggcgccattg ttaaagatgc cgccggtggc tttcattacg atctcttcca gcgtggcatt
    11821 ctcaaaagga gagccgggaa gcagcttgtt caggttcgtg acatacgcct ggtgatgctt
    11881 tccataatgg aactccagag tgttcttcga aataaaaggc tccagcgcat caagcgcaaa
    11941 agggagtgaa ggtaattcgt ggttcataac tctaggatta gttcaaagtt aatagttcaa
    12001 agttcaaagt tagggatttg atatgtaaaa agcaactttg aactttgccc tttgaatttt
    12061 gaactgtctt aataatggca cgcggatgcc gcggatgata cggattctct aattattctt
    12121 aatcagagta tcgagggcga tctggaaaac tttatcgacc tcatggtaaa cgggatagaa
    12181 ggcttcatca tccagcaggt tgcggccgat gtaagctttc agcagtgtct ttatcttctc
    12241 ctttgaatca atgagctcag aaggcgtata gtagatatcc tggttcctgg cataatccat
    12301 cagatcgctg aaaagcttgt catccacctt gaatctctca atgaactctt tcggtgtcga
    12361 atattttgcc agtgacggcc tttcccggtc ggtataattg aaagcatatt gaaagattgt
    12421 attctggtgg atcagcttgc ggtaaagcac ctctttctct tcagtcgcca aagccacata
    12481 aacatcgggc ataattccgc caccgccata taccaccttc ccggcaggag tgtagtattt
    12541 aaccgtatct gttaaagaaa tgctgtcggg attttccatc tccccattgg cataccggtc
    12601 g
//
LOCUS       DAOQXS010000015        12552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5506_length_12552_cov_3.806033, whole genome shotgun sequence.
ACCESSION   DAOQXS010000015 DAOQXS010000000
VERSION     DAOQXS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_12552_cov_3.806033"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PKM34_00965"
     CDS             <1..1381
                     /locus_tag="PKM34_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ82178.1"
                     /translation="TLYLTVESDKNNIGKEDTQDIWYSTLNPDSTWSRRVNIKKPLNN
                     ESNNSVVYCSPDNNSLVVINRYDKSGKSIGAGLSKSERTKGGWTIPTALNIKNYYNDN
                     LYCSSNLSPSQNEMILSLERKDSYGEKDFYISFRQDDDSWSEPLNMGSDINTFGDEGT
                     PFIAADNVTLYFSSNAYPGYGSHDIYITRRLDDTWTKWSKPQNLGPSINTPGWDAYFT
                     IPASGDYSYLVSTDNSFGNEDIFRIKVSEAAKPNPVALITGKVYNLKSSGFIEASITY
                     FDLETNEEIGIARSNPDDGTYKISLPSGHAYSFYARKEGYFSVSENIDLTTLKEYSEI
                     TRDLYLAPIDTGTTIRLNNIFFDYNKTELLEASFFELDRLVNIMNENPQIVIQIAGHT
                     DNMGTEVYNQKLSEDRAKAVYEYLKNKSIALSRISFVGYGESRPVETNDTEEGRAFNR
                     RVEFVIVSN"
     gene            1408..2055
                     /locus_tag="PKM34_00970"
     CDS             1408..2055
                     /locus_tag="PKM34_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82179.1"
                     /translation="MKSELFLFRKFYFGMRVFIGIIFSLILSINAFSQSALKVHLPMG
                     KFYGDAIDSTGFTPIFNVTSFTYQAYATLSENEKVVLFVDAGLGVIGTKQIFENDKYK
                     ARVYGLQLGMYFKYNILKPTLTPYIYTGINLDILFANELYLATISGNNTYRTEVGHRV
                     LFPEYVVGVGFTFLQGGLSLDLQDKIGLISVVNGESIKSNLISIGIIVHATIFDL"
     gene            2045..2509
                     /locus_tag="PKM34_00975"
     CDS             2045..2509
                     /locus_tag="PKM34_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82180.1"
                     /translation="MIYKSRPQKFNSLILSILCINRSDGCWCYGYGPYQQPPDLRYKY
                     PVEVNDTWVTSWVAPPYPTSVTCLSTNTSIGGYTDCIVYNFYLPMKYKSSLAFDYLRM
                     NEEDFPDEIEKKYSGFDIYDYYIPGIGMVGWETYLDGALFVKAVLVDYDVAR"
     gene            complement(2548..3375)
                     /locus_tag="PKM34_00980"
     CDS             complement(2548..3375)
                     /locus_tag="PKM34_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82181.1"
                     /translation="MMWNINRSALIILAALLIEAGALTGQNNNQNDKNFFIRGYAGAQ
                     GKISLIFPVELTEYTQDFYDHLLDEWGNYGYFPDMMNIPPVYSGYGYTLKAGIRLFNV
                     FQVEPWWEMYKSFPLQIRLDAYYYDGYSGYSREIDMNYKFRPMYDALGASLLFVPGSS
                     DKPVFFTFGGGIGSYIGKLSIETDGSETINDNTTPVYDYEEYSGKAIGYHGSIGMTYV
                     PWKYLEMESLLTGRYVKIPEMEDAAGYILTNQYDDNQPVVMQLSGIDFRFGIKFIFP"
     gene            complement(3886..4632)
                     /locus_tag="PKM34_00985"
     CDS             complement(3886..4632)
                     /locus_tag="PKM34_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ82182.1"
                     /translation="MIKCIIVDDEFPARECIKNYLKDYCPDVEVVAQADSANSAYKEI
                     LDHHPDLLFLDVEMPNGSGFDLLRMMNKIDFHIIFVTAHEEYAVMAFRFSAMDYLLKP
                     INIKELVTAIDKVRTEISRKSEAVNLETLKRITQMGPAEWDQIVIKHNEGFKVVYLRD
                     IISCEADSYCTIFHISGNKKFVSSKNLKYYEDILTPHGFQRIHNSFLINLSHVVEYSS
                     EGVVALSEGQLAHLGNTYKKKFMDYFERNK"
     gene            complement(4629..7712)
                     /locus_tag="PKM34_00990"
     CDS             complement(4629..7712)
                     /locus_tag="PKM34_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ82183.1"
                     /translation="MPLRTIAGILILINLACFGQLDNYYLKSYSVSDGLPNNHVRWIT
                     QDRDGFLWLATWDGLARFDGVRFKIYRHDPNDSTSVSFFEIIKVAVDSGNRVWVFAGG
                     RLCRYDRSRDNFIRYNLSHFPTLGPGDGPGEFFDILIDPEGSLLVACGFGFYRYDPES
                     DRFASIHPAGNKAGNFIPSFPGFDNMNNLWLMWVDKTHSDYAEVYKCSYKHGQVLSVV
                     DSFVCDPVNFRSRFTNETIRFNYYLSSAGEAWMASSLGLYYLQNDTFKLSQNKIPKGH
                     FSNTGMILWSGHNNGLMIDYLPENHVDTLFEDGRIETVIAYHHDELGNVWFSDMSEPS
                     VKNGLSHCFRTGNYFRHYLTGLEGDEPYVIYGLYKDRVGNIWAGGRPNTHIVKISPDG
                     VEQQINLPFHPVKYFNVPRNIEPDLNGHLWIAFFSDYLFSLDPGTLQYTDFSGINMTN
                     PTNLSKINYRLVKPYGRDLLFSAGAGKLYKVNTLTGNVITSSAPNGWDIFCLYPVGDN
                     TLWVGLSGRLLLTDFGFNKQEIIPVIDPFYNIEDICPGDSSYLWLALLGGGICRFDTL
                     NHQTSFFTTFNGLAHNTVYSIRKDQAGNLWISHNLGISMFNPVTKSFTNYDEKDGLKI
                     KEFNSEAACQTDQGEILFGGIGGIVSFFPDSIRNQPDDDHSKLMISDFRIANKVYPFD
                     KPVSEMKKVRLPKGTDNFQLEFIKPDFRNGDEIRYRYTLGGRMDKWTETDCDHRRVNF
                     TSLRPGDYEFIVECTDSNGEWANSTMLLITIPPYFYQTIVFRLLLAIVVMVVLGLFFY
                     MKLKQIRLSEHKKQEQLRLETLRGQMNPHFIYNSLNSINYFISLNDRLNANQYITDFS
                     RLMRSIMTNSAQDYITLESEIQAIKDYLALEHLRFSNKFDYEIKTDEALDLVITEVAP
                     SLVQPFIENAIWHGLRYIENRKGFLSVRFIMEGNGNLVCYVDDDGIGRKLSVKLKTGD
                     QKKRNSRGIAIVTERLAIINSMQKSRYSVRVMNMYGDREETGTKVRIEIPFRRSL"
     gene            complement(7847..8902)
                     /locus_tag="PKM34_00995"
     CDS             complement(7847..8902)
                     /locus_tag="PKM34_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNQ82184.1"
                     /translation="MKIPHSTFNIQHSTLMNRITSLFGIKYPIIQAGMIWCSGWELAS
                     AVSNAGGLGLIGSGSMYPDVLREHIRKCKAATDKPFGVNLPLIYKQVEDHIQVILDEG
                     VKIVFTSAGNPAKYTKFFKDHGLIVVHVVANTKFAKKCEEAGVDAIVAEGFEAGGHNG
                     LEETTTMTLIPMVRQVTSLPLIAAGGIATGQQMLAAFALGAEGVQVGSRFVASLESSA
                     HPAFKQKVLEANDGDTALVLKKVIPVRLIKTPFYERVKEAENRSASREELMELLGHGR
                     AKLGMFEGDIDEGELEIGQVAGLIREVVPAAEIVKEIVRDFNLTIGRFDDWAKGLSIV
                     DIRLSIVYCLFYFGFRF"
     gene            complement(9065..9214)
                     /locus_tag="PKM34_01000"
     CDS             complement(9065..9214)
                     /locus_tag="PKM34_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82185.1"
                     /translation="MKPNAEIRNQIMQVVENQMKMDDPPETNQTYKRLIRLGFNEFDS
                     KAMIG"
     gene            complement(9300..9950)
                     /gene="elbB"
                     /locus_tag="PKM34_01005"
     CDS             complement(9300..9950)
                     /gene="elbB"
                     /locus_tag="PKM34_01005"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNQ82186.1"
                     /translation="MKKFAVVLSGCGVFDGAEIHEATLTLLAIAQAGATYEIFAPDID
                     QHHVINHYTHKEMAEKRNVLVESARIARGRISPLSKFDARHFDAIIFPGGFGAAKNLS
                     TVAFDGPEAKVNPDVEAAVKAMHRAGKPIGALCIAPAVIARILGGVEVTIGNDPGTIG
                     AIRKMGGKHVETTHGEVVADKKNKIFSTPCYMLDATIVDIYHGATRIVHEMLNFKC"
     gene            10193..10642
                     /locus_tag="PKM34_01010"
     CDS             10193..10642
                     /locus_tag="PKM34_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82187.1"
                     /translation="MNKRFLIAGLITSLINLLLHGLTYALILKDFYRSYPAGSEEFIK
                     QLHRGPDQLIGWAMAATALAMGYFITLIMKWSGARTFLSGLKYASIAGILFWASVNFG
                     LYASSNMFSLPAVFVDLACSAAVMTLAGASAAWMLGRNPKGWRPFFS"
     gene            10669..10989
                     /locus_tag="PKM34_01015"
     CDS             10669..10989
                     /locus_tag="PKM34_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ82188.1"
                     /translation="MKPINLNNVNSREIIPGYRARFVNSDNMTLAFWDISAGSPMPEH
                     SHPNEQVTTLLEGEFMMTIGGDTFHLKPGSVLVIPPDVSHKGHPLTDCRILDVFYPRR
                     EEYK"
     gene            11083..11862
                     /locus_tag="PKM34_01020"
     CDS             11083..11862
                     /locus_tag="PKM34_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HNQ82189.1"
                     /translation="MTDPSFQIIALTPENIAEYGVCGYKDVKKHLELRRKIDWFHKYF
                     PLGLRIKAVITDTGEYQGMLEYIPGEFAHRPVNAHGYMFIHCIFVGFKSQFKVKGYAS
                     ALLDECISEARLAGMKGVAVVARNGSFMAKKDIFLKKGFTQVDRAQPDFELMAMKFDE
                     RAENPAFKELNPANYPEGITILRSPQCPYSVKNVEAIMLTAQRMNLDPNLIEMDDHVT
                     AQKSPCAFGTFCIIHKGRILSHHPISNTRFENIVKSVKREA"
     gene            12004..12522
                     /locus_tag="PKM34_01025"
     CDS             12004..12522
                     /locus_tag="PKM34_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ82190.1"
                     /translation="MQTKMIGSKIAETRRKLNVSQAQLAERLFISPQAVGKWERGESL
                     PDLIMLNRLAKILGVDLNYFSEDFPSVSNETKFHESPVNQPIKPIFGKQKEKLNWNMS
                     RANWVGADFSGLNNLHEKFSSSNMQHCLFIGSDLSGLLLKGNNVDNCDFSGSGFNNSH
                     IQSSNFARSKFK"
BASE COUNT         3467 a         2893 c         2753 g         3439 t
ORIGIN      
        1 aaccctttac ctgacggtag aaagtgataa gaataatatt ggcaaggaag atacgcagga
       61 catatggtat tccacactga atcctgattc tacctggagc agacgcgtca atatcaagaa
      121 accgctgaac aatgagagca acaacagtgt agtatactgt tccccggata ataactcgct
      181 ggttgtgatc aaccgctatg acaagtcagg taaatcgatc ggcgcgggat tatcgaaatc
      241 ggagcggaca aaaggcggct ggactatccc cacggctttg aacattaaaa actattacaa
      301 cgataacctt tattgttcga gtaacctgtc accctcccag aacgaaatga tcctgtcgct
      361 ggaaaggaaa gattcatatg gtgaaaaaga tttctatatc tctttccggc aggatgatga
      421 ttcatggtcg gagccattga atatgggatc ggatatcaat actttcgggg atgaaggtac
      481 ccccttcata gcggcggata acgtcacgct ttactttagc tccaatgcct atcccggata
      541 cggatcccac gacatttaca tcacccggcg gctcgacgat acctggacca aatggagcaa
      601 accgcagaac ctggggccca gcatcaatac gcccggatgg gacgcctatt ttaccattcc
      661 cgcctccggc gattattctt acctggtatc gaccgataat tcatttggaa atgaagatat
      721 cttcaggata aaagtttcgg aggcggctaa acccaatccg gtggccctta ttaccgggaa
      781 ggtgtataac ctgaaatcct ccgggtttat tgaagccagc attacctatt tcgatcttga
      841 gacaaatgaa gaaatcggca ttgccagatc gaatccggac gacggcacct ataaaatttc
      901 actgccttca gggcatgcct attcatttta tgcacggaaa gagggatatt tttcggtaag
      961 tgagaatatc gacctgacga cgctaaagga atattctgaa atcaccaggg acctgtatct
     1021 tgcacctatt gatacaggaa ccaccatcag gctaaataac attttctttg attataacaa
     1081 gacggaatta ctcgaagcgt cattctttga actcgaccgc ctggtaaata taatgaatga
     1141 gaacccgcag atcgtcattc agattgcggg ccatacggac aatatgggta ccgaagtcta
     1201 taaccagaaa ttgtcggaag accgggccaa ggcggtttat gaatacctga agaataaaag
     1261 cattgccctt tcaaggataa gctttgtcgg atacggagaa tccaggccgg tggaaaccaa
     1321 tgatacggag gaagggcggg cctttaaccg gagggtggag tttgtgattg tgagcaatta
     1381 aaaccaataa tcataaagga gaaaattatg aaatcagaat tgtttctttt tagaaaattt
     1441 tattttggaa tgcgggtatt tataggcatc attttttctt tgattctatc tatcaatgca
     1501 ttcagtcaga gcgctttgaa ggtacatctg ccaatgggca aattttatgg tgatgccatt
     1561 gatagtacgg gatttacgcc tattttcaat gttactagtt ttacttacca ggcatacgcg
     1621 acattatctg aaaatgaaaa agtagttctt ttcgttgatg ccggattagg cgtaatcggc
     1681 actaagcaga tattcgagaa tgataaatat aaagcaagag tctatggttt gcaattgggg
     1741 atgtatttta aatataatat actgaaaccc accctgactc cttatatcta tacgggcatc
     1801 aatctggata ttctttttgc gaatgaactc tatctggcta caatatccgg aaataatact
     1861 tatcggacag aggttggaca tcgcgtgttg tttccggaat atgtagtcgg tgttggtttc
     1921 acattccttc agggagggtt aagccttgat ctacaggata agatcgggct tatttcggtc
     1981 gtgaatgggg aaagtataaa gagcaattta atttcaattg gaattattgt acacgcaact
     2041 atctttgatc tataaatcca ggccccagaa gtttaatagt ctgatattaa gtattctttg
     2101 cattaatcgt tccgacggtt gctggtgcta cggctacggc ccataccagc agccgcccga
     2161 cctgaggtac aaatacccgg tggaagtgaa cgatacctgg gttacaagct gggtggcgcc
     2221 tccatacccg acctcggtaa cctgcctgtc gacaaatacg agcatcgggg gctacacgga
     2281 ttgtatagtg tacaactttt accttcctat gaaatataaa tccagcctgg cttttgacta
     2341 tctcaggatg aatgaagaag attttccgga cgaaattgaa aagaaatact caggctttga
     2401 tatatacgac tattacattc ccggcatcgg gatggtgggc tgggaaacgt acctcgacgg
     2461 agcgctgttt gtgaaagcgg tgctggtgga ttatgatgtc gcccgataga ggctgtttca
     2521 actcaccgca aatgccaaag gccagattta cggaaaaata aacttgatcc cgaaccggaa
     2581 atcgatgccc gaaagttgca tgaccaccgg ctggttatca tcgtactggt tggttaatat
     2641 atagccggcg gcatcttcca tttcaggtat cttcacatac cggccggtca gtaatgattc
     2701 catttccagg tatttccagg ggacataggt cataccaata gatccgtgat aacctatcgc
     2761 ttttcccgaa tattcttcat aatcatacac cggtgtggtg ttatcgttta ttgtttcaga
     2821 cccgtcggtt tcaatgctca gcttgccgat ataactgcca atcccgccgc cgaacgtaaa
     2881 aaacacaggc ttgtcggaag aaccggggac aaacagcagc gaagcgccca gggcatcgta
     2941 catcgggcgg aacttgtaat tcatatcgat ctcccggcta taaccgctgt agccatcata
     3001 ataataagca tccagcctga tctgcaaagg gaatgatttg tacatctccc accagggctc
     3061 cacctggaac acattgaaaa gccttatccc cgctttcagg gtgtacccgt aaccggagta
     3121 aaccggggga atattcatca tgtcgggaaa atagccgtaa ttgccccact catccagcaa
     3181 atggtcgtaa aagtcctgcg tgtactccgt tagttctacg ggaaatatca gtgaaatttt
     3241 gccctgtgcc ccggcatagc cacgaataaa gaagttcttg tcattttggt tgttattttg
     3301 cccggtaagg gcaccggctt caattagcag tgccgccagg atgatcagtg cgcttctgtt
     3361 tatattccac atcattgcca gggtgttgat tacataatta tcgaatattc ttcatgaaaa
     3421 acggcaactc tgacaaattc ctgtcttttt gccatataag agcacaccgg acaggtcaaa
     3481 cggttataat taagcccttt acggtaattc cagaagagaa ttgacccaat atttgacaat
     3541 gcaaagtcgg aaatgaatgg catttcgatg attccgtcgc atgccgggca tttcaatgca
     3601 atctgcagca ctgctttaca atttgtgcaa agaagctttt tctggccaat tttccggaat
     3661 atcgtttcat ttccagcctg gccgcaactg gggcaaatga ggttcacgtt gaatgttaat
     3721 ttgacagatt aaatttatag atgtgtaaat gatttctcag gataatctca ccaatgcaaa
     3781 gcatgaacta atgtaatggt gcattgaggc gatagaatta tgcctcttat cattttttct
     3841 taattgaatg tagctaatga tagggttctt attgagcgat taacttcact tgtttctttc
     3901 aaaataatcc ataaactttt tcttgtaggt attacccaaa tgtgctaact ggccttcgga
     3961 aagggcaacc accccttcgc tggaatactc cacgacatgc gataaattga tcaagaatga
     4021 attatggatt cgctgaaagc catgaggggt aaggatatct tcgtagtatt tcagattctt
     4081 tgaactgaca aactttttat taccacttat atgaaagatc gtgcaatagg aatccgcttc
     4141 acaacttatg atatccctga gataaactac tttaaatcct tcattatgtt ttataacgat
     4201 ctggtcccat tcggcaggac ccatttgcgt tattcttttc agggtttcca ggttgactgc
     4261 ttctgatttc ctgctgatct ccgtcctgac cttatcaatt gccgtcacca gttctttaat
     4321 attgatcggt ttcagaaggt aatccatagc ggaaaagcga aaagccatca ccgcatattc
     4381 ttcatgagct gttacaaaaa tgatatgaaa gtcaatcttg ttcatcattc tgagcagatc
     4441 gaacccgctg ccgttcggca tctccacatc gagaaacaac aggtcgggat ggtgatccag
     4501 gatttctttg taagcactgt tggccgaatc tgcctgggcc actacctcca catcggggca
     4561 gtaatctttc agataatttt tgatgcactc cctggcggga aattcatcat cgacaataat
     4621 gcactttatc ataatgatct tctgaatggt atttctatcc tcacttttgt cccggtctct
     4681 tcccggtcgc catacatatt catgacccgg accgaatatc ttgatttttg catggagtta
     4741 ataattgcca gtcgttctgt cactatggca atgcctctcg aatttctctt tttctgatcc
     4801 ccggtcttta gcttaacgga gagcttgcgg ccgattccat catcatcaac ataacagacc
     4861 agattgccgt ttccttccat gataaacctt accgacagga atcctttacg attttcgata
     4921 tacctgagcc catgccagat tgcgttctca ataaagggtt ggacaagcga cggagccacc
     4981 tcggtgatga ccaggtccaa tgcttcatcc gttttgatct cataatcgaa cttattgctg
     5041 aagcgaaggt gttccagggc cagatagtct ttaatagcct gaatttccga ttcaagggta
     5101 atataatcct gggcggaatt ggtcataata gacctcatca gcctcgaaaa atcggtaata
     5161 tattgatttg cattcagcct gtcgttaagt gaaataaaat agttgatcga attcagagag
     5221 ttgtagatga aatggggatt catctggcca cgtaaggttt ccagtcgtaa ctgctcctgt
     5281 tttttatgct cactgagcct gatctgtttg agtttcatat aaaaaaacag gccgagtacc
     5341 accatgacta caatagctaa caacagtctg aaaactatag tctggtaaaa ataggggggg
     5401 atagtgatga gtagcattgt actatttgcc cactcgccgt tgctatcggt gcattctaca
     5461 atgaattcgt aatcgccggg acgaaggctt gtaaaattga cccggcgatg gtcgcaatcg
     5521 gtctcggtcc atttgtccat tcgtccaccc aatgtatatc ggtagcggat ctcatctcca
     5581 tttctgaaat cgggtttaat aaattcgagc tggaaattat ccgtcccttt cggcaacctg
     5641 acctttttca tttcgctgac aggtttatcg aaagggtaca ctttattggc aatcctgaaa
     5701 tcagagatca ttaatttcga atggtcatca tccggttgat tcctgatgct gtcagggaaa
     5761 aaactgacaa ttccgcctat tcctccgaac aggatttcgc cctggtctgt ctgacaagcg
     5821 gcttccgaat tgaattcctt aatcttcagc ccgtcttttt catcataatt ggtgaagctt
     5881 tttgtaaccg ggttaaacat tgaaattccc aggttatgac ttatccataa atttccggcc
     5941 tgatctttcc ttatgctata aacggtattg tgagccaggc cattaaatgt tgtaaaaaat
     6001 gaagtttgat ggtttaatgt atcaaagcgg caaatacccc ctcccagcaa ggcgagccag
     6061 agataggaag agtcgcccgg acagatatct tcaatgttat aaaaaggatc aatgaccgga
     6121 attatttcct gtttattaaa accgaagtcg gttaacagta acctgccgct caggccgacc
     6181 caaagggtgt tatccccaac ggggtaaagg caaaaaatat cccacccgtt tggtgctgaa
     6241 cttgtaatga cgtttcctgt cagggtattg actttgtata gcttgcctgc accggcagaa
     6301 aacagcagat ccctcccgta gggttttacc agccggtaat ttattttcga cagattggta
     6361 gggtttgtca tattgatgcc ggagaaatct gtgtactgaa gcgtgccggg atcaaggctg
     6421 aacaagtaat cgctgaaaaa ggcgatccaa aggtgtccgt tgagatcagg ctctatattt
     6481 cggggcacat taaaatattt gaccggatgg aatgggaggt tgatttgttg ctcaacgcca
     6541 tccggggaaa tctttacaat atgtgtattc ggacgtccgc cggcccagat gttgccaacc
     6601 cggtccttgt ataatccata aataacgtag ggctcatccc cttccaaacc cgtaaggtag
     6661 tgcctgaaat aattgccggt acggaaacaa tgacttaaac cgtttttcac agatggttcg
     6721 ctcatatcac tgaaccatac atttcccagt tcatcatggt gataagctat gactgtttca
     6781 attcttccat cttcgaagag tgtatccaca tgattttctg gcagataatc aatcatcaac
     6841 ccattattat gccccgacca caggatcatt ccggtattcg aaaaatgtcc cttgggtatc
     6901 ttattctgac tcagcttaaa ggtatcattc tgaagataat ataaaccaag gctggaggcc
     6961 atccaggctt ctccggctga tgacagataa tagttgaacc ggattgtttc attggtaaac
     7021 cggcttcgga aatttaccgg atcgcataca aaagagtcga ctacagataa aacttgtccg
     7081 tgtttataac tgcatttgta aacttcggca tagtccgagt gggttttgtc aacccacatg
     7141 agccaaaggt tgttcatatt atcaaaaccg ggaaaagaag ggatgaaatt tccggctttg
     7201 ttaccggcag ggtgaattga agcaaaccgg tccgattcgg gatcgtaacg gtagaagcca
     7261 aatccgcagg caaccagcag cgagccttcc ggatcgatca ggatatcaaa aaattcccca
     7321 gggccatcgc cagggccgag tgtgggaaaa tggcttaaat tataacggat aaagttgtcc
     7381 cggcttcggt cgtaacggca taacctgccc cctgcaaata cccatacgcg gttacctgaa
     7441 tcgacagcta ctttgatgat ttcaaaaaaa gaaacgctcg ttgaatcatt cggatcatga
     7501 cggtagattt taaacctgac gccgtcgaac cgtgccagcc cgtcccaggt agccagccac
     7561 agaaatccgt cccggtcctg tgtgatccag cggacatgat tattgggaag cccgtcgctg
     7621 accgaatagg atttaaggta ataattatcc aactgaccga agcaggccag attgatcaaa
     7681 ataaggatac ctgcaatagt acgcaatggc aaaatgaaag aataaaggtt tatggtttaa
     7741 aggtaagaaa tttattgtta gcaccaaaat ccaaaacaaa ttccaaattc caagcaccaa
     7801 aatccaaaca aattccaaat tccaagcacc aaaatccaaa caaatctcaa aatctaaatc
     7861 caaaataaaa tagacaatag acaatcgaca atcgaatatc gacaatcgac aatcccttcg
     7921 cccaatcgtc aaatcgccca atcgtcaaat tgaaatcacg cacgatctct ttcacgatct
     7981 ccgcggccgg cacgacttcc cggatcaatc cggcaacctg cccgatctcc agttcgcctt
     8041 cgtcgatatc gccttcgaac atgcccaatt tcgcccgtcc gtggcccagg agttccatca
     8101 gctcttcgcg gctggcgctg cggttttctg cttcctttac gcgttcatag aacggagttt
     8161 tgatcagccg caccgggatg acttttttca ggaccagtgc cgtatcgccg tcattggctt
     8221 ccagcacttt ctgcttaaag gccgggtggg ccgacgattc cagcgacgcc acgaaccggc
     8281 tgccaacctg caccccttct gcgcccaggg caaatgctgc caacatctgt tggccggttg
     8341 cgatgccgcc ggcagcgatc aggggcaaag aagtcacctg tcgaaccatc ggaatgaggg
     8401 tcatagtggt ggtctcttca aggccgttgt ggcctcccgc ttcaaaacct tcagcgacga
     8461 tcgcatcgac acccgcttct tcgcatttct tcgcgaattt cgtgttggcc accacatgaa
     8521 ctacgatcag tccgtgatcc ttgaaaaact ttgtgtactt tgccgggttg cctgccgagg
     8581 tgaacacgat cttcactccc tcgtcgagta taacctggat atgatcttca acctgtttgt
     8641 agatcagcgg gaggtttacg ccgaaaggct tatcggtggc cgctttgcac ttgcgtatat
     8701 gttccctgag cacatcggga tacatcgatc cggagccgat caggcccagc ccgccggcat
     8761 tgctgaccgc cgaggccagc tcccagccgg agcaccagat catgccggcc tggatgatgg
     8821 ggtactttat cccgaaaaga gaagtgattc tgttcattaa tgttgaatgt tgaatgttga
     8881 aggttgaatg cgggattttc attaatgttt agatcgggaa aggaaataat gtttatatat
     8941 ttcattggcc ggttcaaagt cattcaaatg gctcttccgg caatttatta aggtttttta
     9001 tgaaacgttg ttcatcaaaa ggattcctga atttcatgat attgaagatt tcaacagcaa
     9061 cgcactaacc gatcatggct ttactgtcaa attcattgaa accgagtctt attaatcttt
     9121 tgtaagtctg attggtttca ggaggatcat ccatcttcat ctggttttca acaacctgca
     9181 tgatctggtt tctaatttct gcatttggtt tcataaaata aattccctca cttaaaactt
     9241 aacactcaaa acttaacact caaaacttaa cacttacaag aacttcccgc attcaaaatt
     9301 caacatttaa aattcaacat ttcatgcacg attctggttg ccccgtgata gatatccacg
     9361 atcgttgcat ccagcatata gcacggtgtg ctgaagatct tgttcttttt atccgctacc
     9421 acttcgccat gggtggtttc cacatgtttt ccgcccattt tccggatggc gccgatggtg
     9481 ccgggatcgt tgccgatggt cacttcaacg ccgcccagga tcctggctat gacagccggg
     9541 gcaatgcaca gcgccccgat cggttttccg gcgcggtgca tggctttgac ggccgcttcc
     9601 acatcgggat tcacttttgc ttccgggcca tcgaaagcca cagtcgacag gttcttggca
     9661 gctccaaagc cgcccggaaa gatgatggca tcgaaatgtc gtgcatcaaa cttactgagg
     9721 ggactgatcc tgccccgggc aatgcgggcc gattcgacca gcacattgcg cttctccgcc
     9781 atctccttat gagtataatg gtttattaca tgatgctggt caatatccgg cgcgaaaatt
     9841 tcatacgtgg cgccggcctg ggcgatagcc agcagggtca gtgtggcttc atggatctcg
     9901 gcgccgtcga acacgccgca cccggataat actacggcaa atttcttcat aggaatacaa
     9961 gtttagtagt gtaaaaatag ggaaaatgga tgagatgcca tctcattttt tcgagatgga
    10021 gtctcgatga atggagactc catctgggca gattgagatg gagtctcaat aaaatcgtcg
    10081 tccgggaaat taatatttcc tgaccgattg caaacgcatt ttggcaaaat gtcaaacaac
    10141 actggtttgg gagattaata tttaatagtt tggttgtaaa taatttatga ggatgaacaa
    10201 aagattctta atcgccgggt taatcacttc attgatcaac ctattactgc atggactaac
    10261 ctatgcgtta attttaaaag atttttaccg gtcatatccg gccggaagcg aggaatttat
    10321 caaacaactt cacaggggcc ctgatcagct tatcggatgg gcaatggcgg ccactgccct
    10381 tgcgatgggc tatttcatca ctcttataat gaaatggtcc ggcgcaagga cctttctcag
    10441 cggactgaaa tatgcgtcca ttgccgggat cctgttctgg gcttcggtga atttcgggct
    10501 ttacgcctcc tccaacatgt tctccctgcc cgccgtgttt gtagacctgg cctgcagcgc
    10561 cgcggtgatg accctggccg gggcatctgc agcatggatg ctgggacgaa atcccaaggg
    10621 atggcgtccc ttttttagct aactttcgat tctctaaaac aattcaatat gaaacccatc
    10681 aacctgaaca atgtcaattc ccgcgaaatc atccccggct accgggcccg gtttgtgaat
    10741 agcgataata tgactctggc tttctgggat atttctgccg gttcgcccat gcctgagcat
    10801 tcccatccta acgagcaggt gacaactctc ctggaaggag aattcatgat gacaatcggt
    10861 ggtgatacct ttcacctgaa acccggatcg gtgctggtca ttccgcccga tgtcagtcat
    10921 aaaggccatc cattgaccga ttgcaggatc ctcgatgtct tttatccgcg aagggaagaa
    10981 tataagtgat gcagcctctt tccctgagac tccatctcgg cagtttgaga tggagtctca
    11041 attttatatc tttacccgaa ttttaatacc cagaccagga ttatgaccga cccttccttc
    11101 caaatcatcg cccttacacc cgaaaacatc gccgaatacg gcgtctgtgg ctataaagat
    11161 gtgaaaaaac acctggaact gcgacgtaag atcgactggt tccacaaata tttccctctg
    11221 ggattaagaa ttaaagccgt aattacagac accggcgaat accagggaat gctggaatat
    11281 atacccggtg aattcgccca ccggccggtc aatgcccatg gctatatgtt catccattgc
    11341 atcttcgtgg gctttaaaag ccaattcaaa gtaaaaggct atgcttctgc acttcttgac
    11401 gaatgtatct ctgaagcccg gctcgctggt atgaaaggtg ttgctgtagt tgcccgcaat
    11461 ggctccttca tggcaaagaa agacatcttc ctgaaaaaag gatttaccca ggttgatcgg
    11521 gcacaaccgg atttcgaact gatggcgatg aaatttgatg aaagggcaga aaatccggct
    11581 tttaaggaac ttaatcccgc caattatccg gaaggcatta ccatcctccg ttcaccgcaa
    11641 tgcccttatt ctgtgaagaa tgtggaagcc atcatgctga ccgctcagag gatgaatctg
    11701 gatcccaacc tgattgaaat ggatgaccat gttaccgcac agaaatcccc atgcgctttc
    11761 ggaactttct gcatcatcca caaaggcagg atcctgagcc atcatccgat cagcaatacc
    11821 cgttttgaga atattgtgaa aagcgtgaag cgtgaagcgt aaaaacacaa ccaatggttg
    11881 tgctaaacaa ctaatagtat cataattaca accaatcgtg tttagttttt tttgattttc
    11941 gatatgaggc atgatatctt tgccgtatcg aaaaaattat aattcaaata ataaaaatag
    12001 acgatgcaaa ctaaaatgat tgggagtaaa attgctgaaa cacgcaggaa attaaatgtg
    12061 tcgcaggcac aacttgccga acgcttgttc atcagcccgc aagcggttgg taaatgggaa
    12121 cgcggggaat cccttccgga tcttattatg cttaaccggc tcgcaaaaat ccttggagtt
    12181 gacctgaact atttctctga agactttccc tctgtcagta atgaaacaaa atttcatgaa
    12241 tcacctgtta atcagcctat taaacccatt ttcggtaagc agaaggaaaa gctgaactgg
    12301 aatatgtcgc gcgcgaattg ggtgggagcc gatttttcag gattgaataa cctccacgaa
    12361 aaattcagtt catccaatat gcagcactgc ctgtttatag gttccgattt gtcgggactt
    12421 cttttaaaag gcaataatgt cgataattgt gatttctcag gatcaggttt caacaatagc
    12481 catattcaga gttccaactt tgcaagaagt aaattcaaat gacaaagtac ttgttaagat
    12541 attcacagac gg
//
LOCUS       DAOQXS010000016        12433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5557_length_12433_cov_3.777993, whole genome shotgun sequence.
ACCESSION   DAOQXS010000016 DAOQXS010000000
VERSION     DAOQXS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_12433_cov_3.777993"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PKM34_01030"
     CDS             <1..1254
                     /locus_tag="PKM34_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82191.1"
                     /translation="GTEKLFLQVWNWTNETWNAISEYSNFDGSFDWISERILITAKNK
                     VFKVRFLATGENSSAINRWALDNIHIYRTCDGLWDVWLEENPDYNTLEWDGYGGWGWF
                     DGWFNWDDGVFSGNSIGLGEPAEFEFASRWEPAQLIIFPGAVITEVAFVPAEVNADYR
                     IRIWNGSAPDSLLIDQPVQYPVIGEWNYVTLVDTVTIDVTKDLWVGYYVNAQTGYPGG
                     VDDGPAIDGFGNMIYYQDQWQTLKDISDDLDFNWNIACHVIPGLPFPNDKWLYKIYRN
                     TDGGDYLFLDTTFSWNYEDHDIILEEKYCYKVSAVWTENNDTCESDDNPEVCETLCLG
                     VDSPQQDNKVKIYPNPASNWLWIESEDLIREIKLFNSLGETLMNLEIGNLKFAVYCSS
                     LPAGIYVIEVKTQTTTFRSKFLKID"
     gene            complement(1231..1977)
                     /locus_tag="PKM34_01035"
     CDS             complement(1231..1977)
                     /locus_tag="PKM34_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNQ82192.1"
                     /translation="MNKLKKIWAEEYPIYWYDTDCNGQLKMSAVANYLQESAWRHANH
                     LGFGYEDARKRNEFWVVISLMIRMIGQPVWGQKIIVETWPKGVDKLFAYRDFKIMDHN
                     GNVIGAATSAWMILDQETRKPKSVDLVKPVLHLATHQDVLDCNPPLLRPVKEIATTEP
                     RKVRFSEVDQNGHVNNIRYLDWSVDALPEEWHKEHRIRTLTINYLSEVMAGEMMMIST
                     GKAEGNSVVVQGTREDGKVAFRTLIDFQEF"
     gene            complement(2211..2771)
                     /locus_tag="PKM34_01040"
     CDS             complement(2211..2771)
                     /locus_tag="PKM34_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNQ82193.1"
                     /translation="MKKSLKILVIAAIAITASFTVQAQKYMTKNGNIKFYSETPIETI
                     EATNNQVNAALDSQTGDLVFKVLIKSFQFEKALMQEHFNENYMESDKFPNSTFVGKVT
                     NLTAVDFSKEGSYEATIEGDLTIHGVTKKISEKGTFTVKAGDKIHGISKFNVKPADYS
                     IKIPGAVVKNIAETIEVTVDIELSKM"
     gene            complement(2810..3250)
                     /locus_tag="PKM34_01045"
     CDS             complement(2810..3250)
                     /locus_tag="PKM34_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82194.1"
                     /translation="MKKRTIITIILSLFVIGVISAGLVYKFVINKPHENYEKAKPAFI
                     ISATDLYNSFQSDRTTAEGKFNGKVVLLSGKLDKIDLAESLVTGVFVFNQGMFGDEGI
                     RCVMLGNHAQALKAIGPGTEVKIKGYLTGYNDTDVVLEQCSIIR"
     gene            complement(3342..3770)
                     /locus_tag="PKM34_01050"
     CDS             complement(3342..3770)
                     /locus_tag="PKM34_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82195.1"
                     /translation="MKTTAKLFILALTISLASCSKSNDDNPSNTNNTGQVTGNWKVTY
                     YFDSGKDETSDYNGYTFNFNTGNILEAVTGSATYTGTWTLGEPGDDDNSSNKFVITIT
                     GNKAMDDLTDDWKILKMTDTEIWLQDDNLTSAEELRFGKL"
     gene            complement(3920..4678)
                     /locus_tag="PKM34_01055"
     CDS             complement(3920..4678)
                     /locus_tag="PKM34_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ82196.1"
                     /translation="MKLLIIEDEPASAQRLKKMVEEMDPEVKVLGILDSITSTMDWFG
                     HHPEPDLILSDIHLADGLSFEIFRQINISCPVIFTTAYDQYAIQAFKLNSIDYLLKPV
                     RKEELAEAINKFKKVKPAEKQVDLSQLMALIGKQEKEYLRRVVIRLGQQIKIVEIKDV
                     AYFYIDEKIVFGVTFGKDRYPMDLSLDQLEKQLDPGRFFRINRAFIISLDSIETMITY
                     SKARIKIKLKPPCELESITSTERSAEFREWLRGG"
     gene            complement(4787..7663)
                     /locus_tag="PKM34_01060"
     CDS             complement(4787..7663)
                     /locus_tag="PKM34_01060"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNQ82197.1"
                     /translation="MFWLTAFIFLIAHSGFSQLPGFEKIRITVPGEGPVFKKAVMDAD
                     GYIWLGSGQGLFRFDGIQFKSFTPLPDTADFHVTALHLDQDHDLWIGCRDGKIYRLDQ
                     GRINLFNPEEGTSGVAVTDILVAQDRSIWWSTAGEGIYFYSGTRVFNINRDDGLPDDY
                     VHDLESVSDGYVVAGTDGGLVSCIYRNGEKIVNPLAASGELPDIMVTVVKEDSDGNLW
                     LGFQDGGIGFLTNDGRQFIQPAADHAWEEGPVSAVTVSGASAWAGTSSGELLEISLNE
                     NNRGMINLLPADLQTGKIYDLLEDREGNIWILSAEGLFRSTGNRIRYLDDVGGIPLAN
                     IHAIHADEFRPGLLWFSDDRGLFSLAVESGDVKSYPGPFKGPGLKITCLNQDRFGYIW
                     AGTFNYGAIRLNPANGTFDRITERDNLVNNNVLSISRHHDTLWMATLGGASEIILDPN
                     TSGRSYQARSYNYENGLVNNFIYYVYEDDKNRIWLATDGDGINVRTQDGWSSYNENNG
                     LTDDVIYSLTGDKYGNIWAGSASGGLFKFDGKNFIHYGLDEGLSSISFTGIMAADDEL
                     LAVHENGLDILHIPTGNIAHYGGDKGFEGIQPDLNTISRDREGNIWLGTRKGIIRYRP
                     GSAVSYMPVTVLEEMSVYLEPRELKAGQVFGYADNHISFRYAGLWFSNPERVNYQVML
                     EGYDLGWNDTYNRQVTYSSLPPGEFTFKVRSSLDRSFVNAGEVSYSFRIRQPVWIQPW
                     FIILLVIILAALIFLYIKMRENRLRKREQEKKEKIEFEFQVLKNQVNPHFLFNSFSTL
                     ISLIEDNPEQAVQYTEKLSDFFRTILQYKDQELITLDEELTLIQSYFFLLKKRYGDNL
                     YLDIQLGDELKKAFIPPMTLQILIENAVKHNVISMSKPLFIKVYEDDGKIVVENNLQP
                     KLTPEVSTGIGLENIRKRYKLITDKEVIIEISELLFRIFLPLIK"
     gene            complement(7695..8246)
                     /locus_tag="PKM34_01065"
     CDS             complement(7695..8246)
                     /locus_tag="PKM34_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNQ82198.1"
                     /translation="MIKHILFIIIVLLVNNSFCQPVYKTKNGTASFVSNAPLEIIRAE
                     SAEVQGAIDPGKKTFAFSIDNKSFKGFNSPLQQEHFYENYLEVQSFPASTFKGKIIED
                     IDLNSSLEQTVRAKGMLNIHGVEQERIIKGTIRVVDGKISLKADFSVMLEDHQIRIPR
                     VVNQKIAESIDVSVTAELVKSND"
     gene            8375..9421
                     /locus_tag="PKM34_01070"
     CDS             8375..9421
                     /locus_tag="PKM34_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ82199.1"
                     /translation="MKILFIILIVILIIILGSLLLGWLFSAPVYHGPVSDHFDGKKFI
                     NPGGKKAGGLKDVIKWSRERQRGEWKEITEVNYGPAPEERVEGDSIVVTFVNHSTFLI
                     QTQGLNILTDPVWSERVSPVTFAGPKRMRQPGIRFEELPPIDIILLTHNHYDHLDIST
                     LKKLTSRFKPVIYTPLGVGLYLKKKGIDNFREMDWWDELTTPNGLKLVCTPAQHFSGR
                     GMFDRDRTLWSGFALRTENGSIYYSGDTGYGEFFNEIAERLSPIRLSFLPIGAYKPQW
                     FMSPIHTSPADAVNIHKILQSSQSIGMHFGTFPLADDGMEEPAKELKEAKSREGILDE
                     RFLILEEGRAYSFH"
     gene            10553..11638
                     /gene="carA"
                     /locus_tag="PKM34_01075"
     CDS             10553..11638
                     /gene="carA"
                     /locus_tag="PKM34_01075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNQ82200.1"
                     /translation="MNKTVPATLILDDGTEFRGYSFGAEQSSSGEVVFSTAMNGYPES
                     LTDPSYKGQILVATYPLIGNYGVPEMRMKEGMTEFFESDKVQISGLVISDYSFSYSHW
                     NAVKSLSDWLKEHRVPGIAGVDTRKLTRIIREKGAMLGKIVVQDDVHFYDPNKDNLVN
                     QVSAREKITYGNGRRRILLVDCGTKNNIIRCLLKRDATVHRVPWDHPADPAEYDGILV
                     SNGPGDPKMCKATIESVKNLLDGDLPVFGICLGSQIMALAAGADTYKLKYGHRSHNQP
                     VIMKESHRCFITSQNHGYAVDTETLPEGWKPWFENLNDGTCEGIIHSSKPFFAVQFHP
                     EASAGPVDTEFLFDHFMEKVREYEARH"
     gene            11622..>12433
                     /locus_tag="PKM34_01080"
     CDS             11622..>12433
                     /locus_tag="PKM34_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ82201.1"
                     /translation="MKHAIKKVLLLGSGALKIGEAGEFDYSGTQALKALREEGIATVL
                     INPNIATVQTSEGMADKIYFLPVTPFFVEKVIEKENPDGILLSFGGQTALNCGVALHQ
                     SGILEKHGVRVLGTPVSAILDTEDRELFTRKLDQIHVKTPRSIAVTGVDEAVQAAATL
                     GYPVIVRSAFALGGLGSGFCGSEDEMRALTQKSFSYSSQVLVEESLKGWKEVEYEVVR
                     DGFDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNREYHKLRELSIRIVRH"
BASE COUNT         3202 a         2929 c         2950 g         3352 t
ORIGIN      
        1 ggtacagaaa aattattctt gcaggtgtgg aactggacca acgaaacctg gaatgctata
       61 tctgaatata gcaattttga cggcagtttt gattggatca gtgagcggat tttaataaca
      121 gcaaagaata aagttttcaa ggtccggttc ctggctaccg gtgaaaattc gtccgcaata
      181 aatagatggg cattggataa tattcacatt tatcgcacct gtgatgggct ctgggatgtg
      241 tggctggaag aaaatccgga ttacaatact cttgaatggg atggttatgg aggatgggga
      301 tggtttgatg gctggttcaa ctgggatgat ggcgtattca gcggtaattc aatcggtttg
      361 ggtgaaccgg ctgaatttga gtttgcctca aggtgggaac cggctcagtt gatcattttc
      421 cctggagccg taatcacgga agttgctttt gtccctgcag aagtaaacgc ggattaccgg
      481 atcaggatct ggaacggaag cgcacccgac tccctgctta tcgaccagcc tgttcaatac
      541 ccggtgatag gtgaatggaa ctatgtgaca ctggttgata ctgtaactat tgatgtaaca
      601 aaagatctct gggtcggtta ttatgtcaat gcgcagaccg gttaccctgg aggagttgat
      661 gacggtccgg ctatcgatgg atttggaaac atgatctact atcaggacca atggcagaca
      721 ttaaaagata tcagtgacga cctcgatttc aactggaata tcgcatgcca tgtaattccc
      781 gggctgccat ttccgaatga taaatggctt tataaaatct accgtaacac cgatggcggc
      841 gattacctat ttctcgatac gactttttca tggaactatg aagatcatga cattatcctt
      901 gaagaaaaat actgttacaa agtatcggca gtctggactg agaacaacga cacatgtgaa
      961 tccgacgata accccgaagt atgcgaaacg ctttgcctgg gtgtcgattc accgcaacag
     1021 gataataagg tgaaaattta tcccaatccg gcatcaaact ggctttggat cgaatctgaa
     1081 gatttaatcc gggagatcaa actgtttaat tcgcttggag agacgctgat gaatctggaa
     1141 attggaaatt tgaaattcgc agtgtattgt tccagtttgc cggcagggat ttatgttatc
     1201 gaagtaaaga cgcaaactac caccttccgc tcaaaattcc tgaaaatcga ttaatgtcct
     1261 gaacgccacc ttcccgtctt ccctggttcc ctgcaccaca accgagttcc cttccgcctt
     1321 gccggtcgag atcatcatca tctccccggc catgacctcc gataggtaat tgatggtcag
     1381 cgtacggatg cgatgctcct tgtgccattc ctccggcagg gcatccacgc tccagtcgag
     1441 ataacggatg ttgttgacat gcccgttctg gtcgacttcg ctgaagcgca ccttgcgggg
     1501 ttcggtcgtc gcaatctctt tcaccggccg tagcagcggg ggattgcagt cgagcacgtc
     1561 ctggtgggtg gccaggtgca gcaccggttt gaccaggtcg acgctcttcg gctttctcgt
     1621 ttcctggtcg aggatcatcc acgccgaggt cgccgccccg atcacattcc cgttgtgatc
     1681 catgatcttg aagtccctgt aagcaaataa cttatccacc cctttcggcc atgtttccac
     1741 gatgattttc tgaccccaaa ccggctggcc gatcatccgg atcatcaggc tgatgaccac
     1801 ccagaattcg ttgcgtttcc tggcatcttc gtagccaaag ccgagatggt tggcatgcct
     1861 ccatgccgat tcctgcaggt aattggcaac ggccgacatt ttcaattgcc cgttgcagtc
     1921 ggtgtcgtac cagtagatcg ggtattcctc agcccagatt ttcttcagtt tgttcatcaa
     1981 cgctttacga ttattgcagg caaatttatt aattaaccac aaagagacac aaaggacttc
     2041 acgaagtaac acaaagggtt taatttggta atcaattact ttgtggcctt tgtgattcct
     2101 ttgtggcctt ggtggttaaa actttttgag tcatctcagc ataaaaaacc ccggaaagcc
     2161 ctgttaaaag cgatccgggg aggagatata tggggaacag tggtgtgctg ctacattttc
     2221 gacagctcga tatctaccgt tacttcaatg gtttcggcaa tgtttttgac aaccgcaccc
     2281 gggattttga tcgaataatc ggcaggcttc acgttgaatt tgctgatccc gtggatctta
     2341 tccccggcct tgacggtaaa cgtccctttt tcgctgatct tcttcgttac gccgtgaatg
     2401 gtcaggtcgc cctcgatcgt cgcttcataa gacccttctt ttgagaaatc aacagcagta
     2461 agattggtca cttttccgac aaaggtcgaa ttcgggaact tgtcgctctc catatagttc
     2521 tcgttgaaat gctcctgcat gagtgctttt tcgaactgga acgacttgat aagaactttg
     2581 aataccaggt cgccggtttg gctgtcgagg gcagcattta cctggttgtt agtagcctcg
     2641 atagtttcga tgggggtttc ggagtagaat ttgatattcc cgttcttggt catgtatttc
     2701 tgggcctgga cggtgaacga tgcggttatg gctatagccg cgatgacaag gattttaagt
     2761 gactttttca ttttatttga ttcttttgaa ttaataatcg attgaatttt taacgtatga
     2821 tggaacactg ctccaggacg acatcggtat cgttgtagcc ggtcaggtat cctttgatct
     2881 ttacttctgt tcccggtccg atggctttca gcgcctgggc atggttgccg agcataacac
     2941 agcggatccc ttcgtcgccg aacatcccct ggttaaagac aaataccccg gtaacaaggc
     3001 tttcagctaa atcgatctta tcgagtttac cactcagcag caccactttg ccgttgaatt
     3061 taccttcggc tgtcgtccgg tccgactgaa agctgttata caggtcggtg gcagagatga
     3121 taaaggccgg tttggctttc tcatagtttt cgtgaggctt gttgatgacg aatttataaa
     3181 caaggcctgc gctgatcacc cctatgacaa acagggacag aatgatggtt atgattgttc
     3241 tttttttcat ggtttcttta ttttttgcac aaaagcgcta taaatctcta tttactaaca
     3301 ctattaactc tggaacccgg aacattggaa cactggaaca cttatagctt tccgaatctc
     3361 agttcttccg cgctcgtcag gttatcgtcc tgcagccaga tctccgtatc ggtcatttta
     3421 agtattttcc agtcgtcggt caggtcatcc atggctttgt ttcctgtaat ggtaatgaca
     3481 aatttgttac tggagttatc gtcgtcgccc ggttcgccta aggtccaggt gccggtataa
     3541 gttgcggagc ccgtaacagc ttccaggata ttcccggtat tgaaattaaa agtgtacccg
     3601 ttgtaatcgc tggtctcgtc tttgccgctg tcgaaatagt aggttacttt ccagttaccg
     3661 gtcacctgtc cggtgttatt ggtattggaa ggattatcgt cgttgctctt ggagcaggaa
     3721 gccaggctga tggtcagtgc gaggatgaaa agttttgctg tagtcttcat ggtgtttttg
     3781 tttgatgagg caaagtaacg tcgaaccgag ggcgagttcc aaataatatt ggatgaacgg
     3841 gaaaaagtga gacttgaatg gggaagttgg gcagttggac agcctgtccc gagtagagcg
     3901 agggatttgg gcagatggac taacctcccc gcagccactc cctgaactcg gccgaccgct
     3961 cggtgctggt gatagactcc agctcgcagg gcggtttcag cttgatcttg atgcgggctt
     4021 tggagtaggt gatcatggtt tcgatcgaat cgaggctgat gataaaggcg cggttgattc
     4081 ggaagaaccg cccgggatcg agttgcttct ccagctgatc gaggctcagg tccatcgggt
     4141 agcggtcttt gccgaaggta acgccgaaaa cgatcttttc atctatataa aaataagcca
     4201 catctttgat ctccacgatc ttgatctgct gccccaaccg gatcaccacc cgcctgaggt
     4261 attctttctc ctgtttgcct atcagcgcca tgagctgcga taaatcgacc tgcttttccg
     4321 ccggcttcac ctttttgaat ttattgatcg cctcagccag ttcttccttc ctgacgggct
     4381 tcagcaggta atcgatgctg ttcagcttga aggcctggat ggcgtactgg tcgtatgccg
     4441 tggtgaagat aacggggcag gaaatgttga tctgcctgaa gatttcgaaa ctgagcccgt
     4501 cggccaggtg gatgtccgat agaatcagat cgggctcggg gtgatggccg aaccaatcca
     4561 tggtggaggt gatgctgtcg agaatgccga ggaccttcac ctcgggatcc atctcttcca
     4621 ccatcttttt cagccgctgg gcgcttgccg gttcatcttc aataataaga agtttcattt
     4681 tctttatttc atttaattaa ccacaaagta cacaaaggtt tcacaaagga gcacaaagtg
     4741 attgactgcc ggactaaggc cttcgtggat ctttgtagta taataatcat ttgatcaagg
     4801 gtaaaaaaat cctgaaaagc agttccgata tctcaattat gacctcttta tcggtaatca
     4861 gtttatatct tttcctgata ttttcaagtc cgatgccggt ggagacttcg ggagtgagtt
     4921 tgggctggag attattctct acaacgatct tcccatcatc ttcgtaaact ttaataaaca
     4981 aaggcttact catcgatata acattatgct tcacagcatt ttcgatcaag atttgaagcg
     5041 tcatgggcgg gataaatgcc tttttgagct catcaccaag ctgaatatcc aggtaaaggt
     5101 tatctccata acgtttcttc aaaaggaaga aataactttg tatgagtgta agttcttcat
     5161 ccagggtgat cagctcctgg tctttgtatt gcaggatcgt cctgaaaaaa tcagatagtt
     5221 tttccgtata ttgtaccgcc tgttcaggat tatcttcgat gagggagatc agggtactga
     5281 aactgttgaa gagaaaatgt ggattgacct ggtttttcag cacctggaac tcaaactcga
     5341 tcttctcttt tttctcctgt tctctctttc tgagcctgtt ttcacgcatt ttaatgtata
     5401 gaaagataag cgccgccagg ataatgacca gcaggatgat aaaccagggt tgtatccaga
     5461 caggttgcct tatcctgaag ctgtaactga cttcgccggc attgacaaaa gaccggtcga
     5521 gcgaggatct tactttaaag gtaaattcac cgggcggaag gctggaatag gtcacctggc
     5581 ggttgtaagt atcgttccag cccaggtcgt acccttcgag cataacctgg taattcactc
     5641 tttccggatt ggaaaaccaa aggcctgcat accggaatga aatgtggttg tcggcataac
     5701 cgaacacctg gccggccttc aactcccggg gttccaggta aaccgacatc tcctccagaa
     5761 ccgtgacggg catataagaa accgccgacc ccggccggta ccggatgatc cctttccgtg
     5821 tccccagcca tatattccct tcgcggtcgc ggctgatcgt gttgagatcg ggctggatcc
     5881 cttcaaatcc tttgtcaccg ccataatgag cgatgtttcc cgttggaatg tgtaggatat
     5941 ccaggccatt ctcatgaacg gccagaagtt catcgtctgc tgccatgatc ccggtgaagg
     6001 aaatgctgct cagaccctcg tcaagcccat agtgaatgaa gttcttccca tcgaatttaa
     6061 acaatccgcc cgatgccgat ccggcccaga tattgccata tttatccccc gtaagggaat
     6121 agatcacatc atcggtaagg ccattgtttt cattgtatga agaccatccg tcctgcgtcc
     6181 tgacattgat cccgtctccg tcggtcgcca gccagatcct gtttttatca tcctcataaa
     6241 cataatagat gaaattattg accagcccgt tttcataatt gtatgaccgg gcctgataag
     6301 atcttccgga agtattcggg tcgagaatga tttccgaagc tcctcccagg gttgccatcc
     6361 acaaggtgtc gtgatgccgg gatatggata aaacattgtt attgaccagg ttatcccttt
     6421 cggtaattcg atcaaatgtg ccatttgcag gattgagcct gatcgccccg tagttaaaag
     6481 tacctgccca gatgtagcca aagcggtcct ggttaaggca ggtgattttc agtcccggcc
     6541 ctttaaacgg acccgggtaa gattttacat cgcccgattc gacggccagg gagaacaacc
     6601 cgcggtcgtc gctgaaccac agcaggccgg gacggaactc gtcggcatgg atggcatgga
     6661 tattcgccag cggaatcccg ccaacatcat ccaggtatcg aatcctgttc ccggtcgacc
     6721 ggaacagccc ttcggcagaa agtatccaga tattgccttc ccggtcttcc agcaagtcat
     6781 aaattttgcc ggtctgaagg tcagcgggta acagattaat cattccccga ttattctcat
     6841 tcagactgat ctccagtaat tcaccgcttg aagtgcctgc ccacgccgat gcaccggaaa
     6901 ccgtcactgc gctcaccgga ccctcttccc aggcatgatc tgccgcaggc tgaataaatt
     6961 gccgcccgtc gttcgtcaaa aaaccaatcc ctccgtcctg gaaacccagc cagaggttgc
     7021 catcactatc ctctttgacc acagtgacca ttatatccgg caattcaccg gacgccgcaa
     7081 gcggattgac tattttctcc ccgtttctgt aaatacacga aaccagcccg ccatcagtac
     7141 ccgccacaac atatccatcg ctcacagatt caaggtcatg cacataatcg tcgggaaggc
     7201 cgtcgtcacg gtttatattg aagacccggg tgccggagta aaaatagatc ccttcgccgg
     7261 ctgtcgacca ccagatacta cggtcttggg ctaccaggat atcggtgaca gccacaccgg
     7321 aagtaccctc ttccggatta aagaggttga tccggccctg atccaaccgg tatattttac
     7381 cgtccctgca cccaatccag agatcgtgat cctggtcaag gtgaagtgca gtcacgtgga
     7441 aatcagccgt atccggtaag ggggtaaaag atttgaactg aatgccgtcg aaacggaaca
     7501 ggccctgtcc ggaacccagc cagatgtatc catctgcatc cataacagct tttttaaaga
     7561 caggtccttc ccccgggacg gtgatcctga tcttctcaaa gcccggaagt tgtgagaatc
     7621 ctgaatgggc aatcaggaag ataaaagccg tgagccaaaa caaaggactc cacaatctcc
     7681 ttattttcag accattaatc attgcttttt accagttcgg cagtgactga aacgtcaata
     7741 ctttccgcaa tcttctggtt aacgacccgg ggtatcctta tctgatggtc ttccagcatc
     7801 acgctaaaat ctgccttcag gctgatcttc ccatctacca cccggatcgt tcctttaata
     7861 atcctttcct gttcaacgcc gtgaatattc agcattcctt tggccctgac cgtttgctcc
     7921 agtgaagagt tcaggtcgat atcttcaatg atcttccctt taaaagttga ggcaggaaaa
     7981 gactgaacct ccaggtaatt ttcataaaaa tgctcctgtt gcagcgggct gttaaaccct
     8041 ttaaaactct tattatcaat ggaaaaggcg aatgttttct tccccggatc gatggcgccc
     8101 tgcacctctg cagattcggc cctgatgatc tccagcgggg cattggatac aaaggaagcc
     8161 gtgccattct ttgttttata aacaggctgg cagaaggaat tatttaccag taaaacaatg
     8221 atgatgaata agatatgttt gatcaccgta gagattttaa agcgaagata atatattcct
     8281 gataaaagtt gattccgtaa tgaaccgatg tatcagcgga ttgtttagac cagattaaac
     8341 agtttatact tttgcattcg attaaaacca aactatgaag atattgttca tcattctcat
     8401 tgtaattctg atcattattc tgggttccct tttactcgga tggttgtttt cggcgcctgt
     8461 ttaccacggt cctgtcagcg accatttcga cgggaagaaa ttcatcaatc cgggtggaaa
     8521 gaaagcggga gggctgaaag atgtcatcaa atggtcgagg gaaaggcaac ggggtgaatg
     8581 gaaagagatc accgaagtaa attatggccc tgccccggaa gaaagagtcg aaggtgattc
     8641 tattgttgtg actttcgtca accattccac cttcctgatc cagacgcagg ggttgaatat
     8701 cctgaccgat ccggtctgga gcgaacgggt cagccctgtg acgtttgccg gcccgaagcg
     8761 gatgcgccag ccgggaatcc ggtttgaaga acttcctcct atagacatta ttttattgac
     8821 ccataatcac tacgaccatt tggatatcag taccttaaaa aaattgacaa gccgttttaa
     8881 accggtgata tacacgccgc tcggggtggg cctttactta aagaaaaagg gcattgacaa
     8941 tttcagggaa atggattggt gggacgaatt gacaacgcca aacgggttga agctggtttg
     9001 tacgccggcg cagcatttca gcggccgggg gatgttcgac agggacagga cgctctggtc
     9061 aggatttgca ttacgcaccg aaaacgggag catatattat tcgggagaca ccggctatgg
     9121 agagtttttc aacgaaatag ccgaacggct ctctccgatc cggctttctt tccttccaat
     9181 cggggcctat aaaccgcagt ggttcatgtc gccgatccac acatcaccgg ccgatgcggt
     9241 gaatatccac aagatccttc agtcctctca aagcatcggg atgcatttcg gcacatttcc
     9301 gctcgccgat gatgggatgg aagagccggc gaaagagctt aaggaagcaa aaagcagaga
     9361 gggaatttta gatgagagat tcctgatact tgaagaaggg cgagcatata gttttcattg
     9421 aggtgtttct tggagatggc atctcaatat gcaaaagaaa ttttctaaat aagctgattt
     9481 gcgggtgaaa agaccggatc ttatttgatt ttgagatgcc atctcaaaga attagatttc
     9541 atattctaaa ctgcttattt ataatcacat atcgcttcca ggaacttgta atcctgatgc
     9601 catctaaaaa atatttttaa gggtccgggt attttgtagt caaattacgg attaacaatt
     9661 gagaattcat ccttttgtta aacctataat caatttacaa tgaaaaagca atctttaaag
     9721 acacctctaa ttgcggggaa gtattatcat ttattcaaca ggggaaataa tagagataga
     9781 cttttctatt tcgattgcaa ttatgacttt tttattaaaa agtacttcta ctatatggat
     9841 ccggttgctg aaacattttg ctattgtctg ttaccaaatc attttcattt tttaattcgt
     9901 ataaaggatt cagcaaccga ttcaaggtat gtttcaaatc aactaaggaa gctgtttata
     9961 acttatgcaa tgtatattaa tcgtcaagaa tgcagaactg gctgtattat tagtaaaaac
    10021 ttcaggcgaa ttgaggtaaa tcgcgacgaa tactttatta gcctagtgcg atacatccat
    10081 ttaaatccga taaagcataa gattactaac gatatttata cttatagata cagtactttt
    10141 tacaaaattc tccgcaatca aatcaatgat tttgagtctg cggaattaat taattggttt
    10201 ggaggatact ccaatcttat tgaagatcat ctgattataa agaatgataa aaaaattaat
    10261 ggttttaaac tggaagacta gtcttggatt ttactgagta ataaggataa taaacttgga
    10321 gttcagaagg ttgattttct tttaaataga aataataagc accattttat gagatggcat
    10381 ctcaaatcaa tagatttctt ctttaaataa tttattatgt gtttgttagg aagcattttc
    10441 atgatgtcag agatgccatc tcaattactt ttcaacaatc cgaaatatct ttatcaaaac
    10501 atatattttt agatccggga aatcatataa ttgaatatca actgcctggt ctatgaataa
    10561 aacggttcct gcaacgctta tcctggatga tggcacggaa ttccgcggct attccttcgg
    10621 cgccgaacaa tcttcatccg gcgaggtggt cttctccacc gcaatgaacg gctacccgga
    10681 aagcctcacc gacccgtcgt acaaaggaca gatcctcgta gccacctacc cgcttatcgg
    10741 caattacggg gtgcctgaaa tgcggatgaa agaaggcatg acggaattct tcgaatcgga
    10801 taaagttcag atctccggcc tggtcatttc cgactactct ttcagttaca gtcactggaa
    10861 tgcagtaaaa agcctgtcgg actggctgaa agaacaccgg gtgcccggca ttgccggtgt
    10921 ggatacacgg aaactgaccc ggatcatccg ggaaaaaggg gccatgctgg ggaaaatcgt
    10981 agtgcaggat gatgtacatt tttacgaccc gaacaaagac aaccttgtca accaggtttc
    11041 ggccagggaa aaaataacct acgggaatgg ccgtcgtcgt atcctcctgg tggattgcgg
    11101 cactaaaaac aacatcatcc ggtgcctgct gaaaagagat gccactgtgc accgggtccc
    11161 ctgggaccac ccggcggatc ccgcggaata cgacggcatt ctggtctcca atggtccggg
    11221 tgatcctaag atgtgcaaag ccaccataga atcggtgaag aacctccttg atggcgatct
    11281 gccggtcttt ggcatctgcc tcggctcaca gatcatggcc ctggccgcag gcgccgatac
    11341 ctacaagttg aaatacggtc accggagcca caaccagccc gtaatcatga aagagagcca
    11401 ccggtgcttc ataacttctc agaaccacgg ctatgctgtt gatacagaga cactcccgga
    11461 gggttggaaa ccctggttcg agaatctgaa cgacggtacc tgcgaaggca tcatccacag
    11521 ctcaaaacct ttcttcgccg tacagttcca cccggaagcc tctgccggtc cggtcgatac
    11581 cgaattcctg ttcgatcatt ttatggaaaa agtcagggaa tatgaagcac gccattaaaa
    11641 aagtcctcct gctgggatca ggtgccctga agatcggcga agccggcgaa ttcgactatt
    11701 ccggcaccca ggcgctcaaa gcccttcgcg aagaaggtat tgctaccgtc ctgatcaatc
    11761 ccaacatcgc cacggtccag acttccgaag ggatggccga taagatctat ttcctcccgg
    11821 taaccccttt cttcgtcgaa aaagtcattg aaaaagagaa tccggatggc atcctcctgt
    11881 cgttcggcgg gcagaccgcc ctgaactgcg gcgtcgcctt gcaccagtcg gggatactgg
    11941 agaaacacgg ggtcagggta ttgggaacac ccgtcagcgc catcctcgac acggaagaca
    12001 gggaactttt tacccggaaa ctggatcaga tccatgtaaa aacaccgcgc agcatcgcgg
    12061 tgaccggtgt ggatgaggcg gtgcaggcgg cggcaaccct gggttatccc gtgatcgtcc
    12121 gctcggcttt tgccctcggc ggactgggca gcggcttctg tggatcggaa gacgaaatga
    12181 gggcgctgac acagaaatcc ttctcctatt ccagccaggt gctggtcgaa gaatcgctga
    12241 aaggatggaa agaggtggaa tacgaggtgg tacgcgacgg tttcgacaat tgcatcacgg
    12301 tgtgcaatat ggagaatttc gatccgctgg ggatccacac gggtgaaagc attgtagtag
    12361 caccttcgca aaccctgagc aacagggaat accacaagct ccgggaacta tcgatccgga
    12421 tcgtcaggca tat
//
LOCUS       DAOQXS010000017        12200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5682_length_12200_cov_4.452944, whole genome shotgun sequence.
ACCESSION   DAOQXS010000017 DAOQXS010000000
VERSION     DAOQXS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5682_length_12200_cov_4.452944"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4158
                     /locus_tag="PKM34_01085"
     CDS             <1..4158
                     /locus_tag="PKM34_01085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82202.1"
                     /translation="RTSNIVNVIISTCTGGGGPSGCPPNPVPDCPTVCFSTNVLCGQV
                     QLFNSSYVVPNASIQSYTWSVSPNTGIFTPSNTAPNPTLTVPTSGTYTITLTVISTTN
                     CTASASQAVTVFVPTASFTVSSPVCENSFATFTASPILPGLNYFWTFGDGSTSYSAIT
                     QHSYASAALSPYTVSLTISDNYGCTANYSAPVTVNPNPVCTISSPDTAFCPGGFVPLT
                     ACTGMSSYQWYLNGSPISNANGQTHNANQVGEYYCDVTNSAGCSGISNKLFIYMHKRP
                     KADITGDGYYCSLPGGTIYFSLSTIYNANYSYSWTCIPGGATFSPSNSDNTWVSLTVP
                     LTLPAYFQFVVDVTDNITQCVNSDTLCVTVYETPSLSILSIPALDVCEGTPVTLIPNL
                     NNTGLYSYMWSNGATTPVITVSSAGFYSLTITNKATGCSATANAGAIHPKPDLSLFPI
                     GCDELCDPDSLHLYIPLPLNALPPFNTYAAAYPSITWLDYGSPVGSGPTLAFPAGTSG
                     NHQFSVAVMNQFGCVDTAGVFCLTNGCCNIDLEYLEPTSASCATSADGSFTIVLDPAS
                     TGGPFTITSIPLMPPLPTTIIPGNPLTVGNLPPGTYTIIINGPTEGCTETFFVDIGYI
                     EEECCFAEIDSTFTKILVNTTYTTDVVWDGKYFIDDNVIVTVTNGAVLDITNVDCVFG
                     ECAGIVFTNGAMLRSTNSVYRPCFVDDTWKGLRFTSKGEFDNIINECTFKNAEVALYF
                     QGNADGVISSNLFSNCNYGIRVEGNNNFNHPVTGNHFVTEQFFPNYDCPTKYSFINNS
                     SSYGIYTTTSRFKQQVSQNEFVNTWGNDNPRTHGIYQVNGGATISYNTFTDLSYSIYL
                     STALFTTIIENNEIEINEVVNTTVAPIYVNTTTSPLIEINNNEISDNYHQFNCFSAIY
                     LKSSANVSAINNRIDGFQYGIYANSVRNTQISNNEIIDSDVIGIYFSTKGIFKNYITC
                     NNIKMRSFTNTRGIYTVDMTTRSEISSNCINDCYVSMDIRSFTGAALPKIRNNFLYNY
                     NFVGINVVGHTGNIGTLSPADPGLNTLWSNYNPAIDINSNTNITVADNFGMFNISWPT
                     VQITSNRPFHSTASCAQQIFNMPSQGNLNINYTCDNFKNLFTAFTGVSAPFALAPDYR
                     EQLQVSEYPYEEADLLLAAVEPTDPALVDELIQLPALSDNEKSLLQYNYAYRNADFAV
                     AGTHLASYSPTDEEGTDFKILALYDLDIIMYGRDILTGGDIEIIRAIAEKLSVNSNFA
                     ITLLNNSPEYRDHLFEPVPLPEVLAGADIKRIGEEAMHLVIRPNPASDKVFIESVFSP
                     LSEVRVEMHDVSGKLITGYSLQRAAGGIELDISRLREGFYFVVLTDPATGTVKTGKLI
                     KVKSPGN"
     gene            4204..6081
                     /locus_tag="PKM34_01090"
     CDS             4204..6081
                     /locus_tag="PKM34_01090"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNQ82203.1"
                     /translation="MVFLLAGWAGGQSASPLQGGETIANAFVITGTLPFSASGTTLGY
                     TDDYDEACPYDSSQAPDVVYSYTPAASVIVDIDLCGSEYDTKIFVYENTVTPGIPYDC
                     NDDFYYEEPCGLYVSKIEGLLLTGGNTYYIIIDGYSSGDFGNYDLSVTQSGNTPQCII
                     GINFICPAWAVDEPEDCGQDENGGCDMIPGNESWESVPSAGATYCGSLFADNGDKDSD
                     WYELVLSEPSSVVLTANAEQQISYGMAETTTPGAPTCITLTGDTEPGAVAGPCSETSV
                     DLGTLAPGTYWLVIEMTATSGFPCSNHYVFDIDVNPVPCPPPDFLTATGITSFGATLG
                     WFETGTAASWEYQYGVSGFTPAGSGISTVANPVAVTGLAISTAYDFYARAVCGPGIYS
                     AWSGPYTFTTLCGTINSIPWSEGFESGWPPACWTDDNALPGYGWDHGVYGSPGSGSKW
                     AYSNLSGSVLTSPSISLSADAYLFFSYRAENKDFPRNISVKINNDEVFQVNGFTSEDY
                     SVGAVSLATYIGQTVAIAFETTAGSGGQDFGICLDDVTVKFAVKWTGNLGTAWSNPGN
                     WSTSSIPGINDVVVVPSVPSGGNFPEVGSGITATCYFLELAPGATLKVKTGGTLNVLN
                     Y"
     gene            6334..7461
                     /locus_tag="PKM34_01095"
     CDS             6334..7461
                     /locus_tag="PKM34_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82204.1"
                     /translation="MYKRDIENTIKEWLPGKEVVILYGPRQSGKTTLLTMLFDQNPDA
                     LILNCERPDLADILESSDLSRIKMIFGDKKIIALDEALKIRNIGPLLKLVYDEPSFVQ
                     KIIATGSSSFELANTISEPLTGRNVKFLLLPLSIGELSGARGWLWIYENLNLMPIYGS
                     YPGIIDISGEFKTRKLFDLASDYLFKDILIHENLRNPSLLRKLLKLLALQVGSQVSFH
                     ELSKTLAVSSKTIEKYIDLLEKSFVIFSLPSFSRNQRTEIRKSRKFYFYDLGIRNALI
                     TNFNDPDSRTDTGAIWENFCILERLKYNLIHQPFAQLYFWRTYDGAEIDLVEEANGKI
                     NIFEFKWRGKSNARFPRSFSENYEINTGKLISKENIHELIA"
     gene            complement(7489..8460)
                     /locus_tag="PKM34_01100"
     CDS             complement(7489..8460)
                     /locus_tag="PKM34_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNQ82205.1"
                     /translation="MNNLETIIAETFSKIDFHKIKDHPNILIAARFWEDERYEAAATI
                     YRFMRYIDDMIDDRKALDGMLSCMEKKIYTDQVNAWIDCLGLDRTNDPFFGEVTETIR
                     RFRIPLHFFYNFARSMVYDINHDGFRTFDDFLDYAEGASNGPASVFVHLCCLDKQQGE
                     YVPINLSISDVARPCAIFSYLVHIVRDFQKDQFNNLNYFALDLLEKHGLTAGDLREIA
                     RGSAIPDGFRELIRDYKEQAGKYKTDTEKMIRSLEGRLDHRHMLSLRIIYHLYLQIYN
                     RIDPDNGTFTMAELNPGPEEVKEAVAECIRVNFSERTPKVCPEIHPH"
     gene            complement(8483..9979)
                     /gene="crtI"
                     /locus_tag="PKM34_01105"
     CDS             complement(8483..9979)
                     /gene="crtI"
                     /locus_tag="PKM34_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNQ82206.1"
                     /translation="MKTVAIIGAGAAGLSAANYLAIKGFRVTIFEKNAFPGGRCASFE
                     KEGHRFDIGATLLMMPQVYERMYADFGRNMRDELDLIRMDPVYRLRYADGSELKFTSD
                     LMRMQDQLEAMEPGSYTAFLRYMDKSFKAYKVSMETIIDRNYDNIFQFINPVNLFRLF
                     RLNAFGNHYRTSARYFKNENLRTAFTFQNIYVGQNPFEAMAIFSMLPFMELTDGVYFP
                     KGGMNRVSESLEKIAIGNGVEIQYRANVSGIRVAGERVAGIELADGTFYEADIVLANA
                     DLPYVYQELLPSGPQANRIKKLGYTCSAIVFHWGMDTVLPGIEQHNVYVSDNYRNNIE
                     EVFNGSGFAPEPSFYLHSPARADKSAAPEGHDSISVIVPVGHMGNGKFGDLGIDWEVK
                     KEEARAAVLKRLAMEGFADFEKHIKFEKVYNPVIWKNMFNLSKGATFGSLNHNLMQMG
                     YFRPHNQHNKYKNLFFAGGSTHPGNGVPLALISARLTAEKIIGVNGER"
     gene            10154..10726
                     /locus_tag="PKM34_01110"
     CDS             10154..10726
                     /locus_tag="PKM34_01110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNQ82207.1"
                     /translation="MKNNLILLVLLSLSTFLSAQEIKTKEPIKPALLVIDIQNKFLPM
                     MDAKDREMGMLYIKGYIDIFRRHNLPVICIHHTDPKEGPFPGTPEFAFPDDIGIAKDD
                     PVVVKTYANGFTKTDLDKVLKEKGVNTLFLCGLSSVGCVLATYIGATDHDYKAFLLKD
                     AIFSHKSEYTDQVETIFDAVGWDVVKLAIE"
     gene            10843..>12200
                     /locus_tag="PKM34_01115"
     CDS             10843..>12200
                     /locus_tag="PKM34_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ82208.1"
                     /translation="MKSNFLKLLSLLFSPSPFLLLSLFPLLLFAQTPLPSNWDSIVAS
                     PWYTGNVSNIPAFCYLNPEDPDTGYFFIADKALPQVIPVSVKWKKGVPRSAAFTYQSD
                     KIKVKFTGTVSPDTIAGRIRTSRKSAIRLGISPELEILLIKEIPQIPQSPNLQIPKSP
                     NPPTSLLPRYLSPIFKKVTVTKDIAYGAAPGHYVSMPIEKDSDYDYQQIILDALKNMW
                     VNPGKKALLHLFNRDPVSFAVSDLQGLKMDLYEPEGDTLSFRPLILLLHGGAFILGDK
                     ATESIAELANDYARKGYVVASINYRIGFNPASKSSLERAAYRSVQDARAALRFLSYNA
                     AQYKIDPGHILLGGSSAGAITALNTAFMDEDERPESTGGNIWQLQKDLGGLDESGNDI
                     SGQYTIRALLNLWGAVNDTAIIDAGEKIPVLSVHGDADKIVPYGYNYPFLDMDTNVTS
                     NIV"
BASE COUNT         3081 a         3348 c         2718 g         3053 t
ORIGIN      
        1 cgcacttcca acatcgtgaa tgtcattata tctacttgca cgggtggcgg tggcccgtcg
       61 ggctgcccgc ccaatcccgt acccgattgc ccgaccgtat gttttagtac caacgtgctc
      121 tgcggccagg tccagctttt caactcatcg tacgtggtac cgaatgcatc gatccagagc
      181 tacacctggt ctgtcagccc aaatacgggc atcttcacac ccagcaacac cgctccgaac
      241 ccgactttaa cggtgcccac atccgggacc tacaccatca ccctgacggt tatttccacc
      301 acaaactgca cggcaagcgc ttcgcaggcg gtaaccgttt tcgtcccgac ggcaagtttt
      361 accgtctcct cgccggtgtg tgagaactca ttcgccacct tcaccgcttc acccatttta
      421 cccggcctga actacttctg gaccttcggc gacggcagca catcctattc cgctataacc
      481 cagcattcat atgcttccgc cgctttgtca ccttacaccg tatcattgac catttccgac
      541 aattacggct gcacggccaa ttattcggcg ccggttacgg tcaatcctaa tccggtctgc
      601 acgatttcgt cacccgatac cgctttctgc ccgggaggct tcgtgccttt gaccgcctgc
      661 accggaatga gctcatacca atggtacctg aatggcagcc cgatcagcaa cgcgaacggc
      721 cagacgcaca atgcaaacca ggtgggagaa tactattgtg acgtaaccaa ttcagccggc
      781 tgcagcggga tttcgaacaa gctgttcatt tatatgcaca aacgaccgaa ggcagatatc
      841 accggtgacg gatattattg ttctctgccc ggcggaacga tctatttcag cctgtcgacc
      901 atctacaacg ccaactacag ctatagctgg acctgcattc ccggcggagc gacattttcg
      961 ccttccaatt ccgacaatac ctgggtttcg ctgactgtcc cgcttacatt gcccgcctat
     1021 ttccagttcg ttgtcgatgt tacggacaac atcacccaat gcgtcaattc cgacacattg
     1081 tgcgtcaccg tgtatgaaac accgtcactc agcatcctga gcatcccggc gctcgatgtc
     1141 tgtgaaggaa cccctgttac ccttatccca aaccttaaca ataccgggct ttatagttac
     1201 atgtggagca acggggctac aactccggtg atcacggtct cctctgccgg attctacagc
     1261 ctgacgatca caaacaaagc gacgggttgt tctgccacag ccaatgccgg cgctattcat
     1321 ccaaaacccg atctgagcct tttcccgatc ggttgtgatg agctctgcga tcccgattca
     1381 ctgcaccttt atattccgct accgctgaat gccctgccgc ctttcaacac ctacgccgcg
     1441 gcttatccca gcattacctg gctggattac ggctcacccg tcgggtccgg ccccacgctg
     1501 gcattcccgg cagggacatc gggcaaccac cagttttcgg ttgcagtaat gaaccagttc
     1561 ggctgtgtcg atacagccgg cgttttctgc ctcaccaacg gatgctgcaa catcgacctg
     1621 gaataccttg aaccgaccag cgcttcctgc gccacctcag ccgacggctc ctttaccatc
     1681 gtcctcgatc cggcaagcac cggaggcccg ttcacgatca cctcgatccc gctgatgccg
     1741 ccgctgccca ccaccataat accgggaaat ccgctgactg tcggtaacct gcctccgggc
     1801 acctacacga ttattatcaa tggcccgaca gaaggttgta cggagacatt cttcgttgat
     1861 attggctata tcgaagaaga atgctgtttc gccgaaatcg actctacatt caccaaaata
     1921 ctggtaaata caacctatac gaccgatgtg gtctgggacg ggaagtattt tatcgatgat
     1981 aacgttatcg tcacggtcac caacggggct gtgctcgata tcaccaatgt cgactgcgta
     2041 tttggcgaat gtgcgggaat cgtatttacc aacggcgcta tgctccgctc aaccaattcg
     2101 gtttaccgtc cctgctttgt cgacgacacc tggaaagggc tccgatttac cagcaaaggt
     2161 gaattcgaca acattatcaa cgaatgtacc ttcaaaaatg ccgaagtggc cctgtacttc
     2221 cagggtaatg ccgatggggt catctcatcc aacctcttct ctaattgcaa ttatggaatc
     2281 agggtagaag gcaataacaa tttcaaccat cccgttaccg ggaaccactt cgtgaccgag
     2341 cagttcttcc cgaattacga ctgcccgacg aaatactctt ttatcaataa ttccagttcc
     2401 tatggcattt acaccaccac ttcgcgcttc aaacagcagg tctcgcaaaa cgagtttgta
     2461 aatacgtggg gaaatgataa tccgaggaca catggaatct accaggtgaa cggtggagcc
     2521 accatctcat acaatacctt caccgatctg tcttattcca tctacctgag cacggcattg
     2581 ttcaccacga tcatcgaaaa caacgagatc gaaataaacg aagtcgtaaa caccaccgtc
     2641 gcaccaattt acgttaacac gaccaccagt ccgctcatag agatcaacaa caacgagata
     2701 tccgacaact atcaccagtt caactgtttc tcggccattt acctgaaaag ctcggcgaac
     2761 gtaagcgcga tcaacaaccg gatcgacggg ttccagtacg ggatttatgc aaatagcgtc
     2821 aggaataccc agatcagcaa taacgagatc atcgacagtg atgttatcgg gatctatttc
     2881 agcacaaaag gcatctttaa aaattatata acatgtaata atataaagat gaggtcattc
     2941 accaatacaa ggggtatcta taccgtcgat atgaccaccc ggagcgagat cagcagcaac
     3001 tgtatcaatg attgttatgt ttcgatggat atcaggtcat ttaccggtgc ggcccttccg
     3061 aagatcagga ataacttcct ctacaactac aattttgtgg ggatcaacgt ggtggggcat
     3121 accggcaata tcgggacttt gtcgccggcc gatcccggcc tgaatacgct gtggagcaat
     3181 tacaatcctg cgatcgacat caacagcaat accaacatta ccgttgccga caatttcggt
     3241 atgttcaaca tctcctggcc aacggtgcag atcaccagca accggccgtt ccactccacg
     3301 gcatcctgcg cccagcagat cttcaacatg ccgtcgcagg gcaacctgaa catcaactat
     3361 acctgcgata atttcaagaa cctattcact gccttcaccg gggtcagcgc accgttcgcc
     3421 ctggcccccg actacaggga acagctccag gtttctgaat atccttatga agaggccgac
     3481 cttctcctgg ctgccgttga acccacggat cccgctttgg tcgatgagtt gattcagttg
     3541 cccgctctga gcgacaatga aaaatcgctc ctgcagtaca attatgctta ccggaatgcg
     3601 gactttgcag tggccggaac gcacctggca agctattctc ccacggatga agaaggcacc
     3661 gatttcaaga tccttgccct ctacgacctg gatatcatca tgtatggccg cgatatactc
     3721 accggcggcg acattgagat catccgggcg atcgccgaaa aactgtcggt caattccaac
     3781 ttcgccatca ccctgctgaa caattcgccc gaataccggg atcatctctt tgaacctgtt
     3841 ccgttgcctg aggtattggc cggagctgat atcaaacgga tcggggagga ggccatgcac
     3901 ctggtcatcc ggcccaaccc ggcatccgat aaggtgttta tcgaatcggt cttcagcccg
     3961 ctgtccgagg tccgggtcga aatgcacgac gtgagcggca agctcatcac cggctattcc
     4021 ctgcaaaggg cagccggagg tatcgagctg gatatcagcc gcctcaggga agggttctat
     4081 ttcgtggtcc tgaccgatcc ggcgaccggc acggtgaaaa ccgggaagct gatcaaagtg
     4141 aaatctcccg gtaattaatc cttagcattc ggtcgtttga aaacaacatc gctaatatat
     4201 gttttggttt ttctccttgc gggatgggca ggcgggcaat ccgcttcccc tctccaggga
     4261 ggcgaaacca tagccaatgc atttgttata accggaaccc tgcccttctc ggccagtggt
     4321 actacgctgg gttatactga cgattatgat gaagcctgcc cttacgacag cagccaggca
     4381 cccgatgtgg tctattccta caccccggct gcttctgtta tcgtggatat tgatctctgc
     4441 ggatcggaat atgacacgaa gatctttgtc tatgaaaata ccgtaactcc cggtattcca
     4501 tatgattgta atgatgattt ctattatgaa gaaccctgcg ggctttatgt ttcaaagata
     4561 gaagggcttt tgctcaccgg gggaaatacg tattacatca ttatcgacgg atacagttcg
     4621 ggcgattttg gcaactatga tctttcggtc actcaatcgg gtaacacgcc ccaatgcatc
     4681 attggtatta atttcatctg cccggcctgg gcagttgacg aaccggagga ttgcggccag
     4741 gatgagaacg gcggctgcga tatgattccc gggaatgaaa gctgggagtc ggttccgtct
     4801 gccggggcca cctattgcgg cagtttattt gccgacaatg gagacaagga ctccgactgg
     4861 tatgagctcg ttttgtcaga accttcgtca gtagtcctga cagccaatgc agaacagcaa
     4921 atttcttacg gcatggccga aaccacaacc cccggcgcac cgacctgcat tactctgacc
     4981 ggagacacgg agcccggggc tgtggccggc ccttgcagcg aaacatccgt ggacctgggc
     5041 acccttgcgc ccgggactta ctggttggtt atcgaaatga ccgccaccag cggtttcccg
     5101 tgtagcaatc actacgtctt cgatatcgat gtcaaccccg tcccctgccc gccccctgat
     5161 tttctcactg ctacagggat cacctctttt ggtgccacac tgggctggtt tgagaccggc
     5221 acagcagcat cctgggaata ccagtatggc gtgagcggct ttaccccggc aggttcgggc
     5281 atttcgacgg ttgccaatcc ggttgccgtt acggggctgg cgatcagcac cgcttatgat
     5341 ttctatgcca gggcggtttg cgggcccggt atctacagcg cctggtcggg accttacacc
     5401 ttcacaacgc tatgcggtac aataaactca atcccctggt cggaaggatt tgagtccggc
     5461 tggccgccgg catgctggac cgacgataat gccttacccg gctacggctg ggaccatggc
     5521 gtttacggct cgcccgggag cggctcgaag tgggcttaca gcaacctttc gggttccgtg
     5581 cttacctctc cttccatcag cctgtcagcc gatgcttacc tgttcttcag ttaccgggct
     5641 gagaacaagg atttccccag gaatatatct gtaaaaatta acaatgatga agttttccag
     5701 gttaacggtt ttacctctga ggattattcc gtcggtgccg tttcactggc tacctatatt
     5761 gggcaaaccg ttgcgattgc gttcgaaaca actgcaggaa gcggcgggca ggacttcggc
     5821 atttgcctcg acgatgttac ggtgaagttc gctgtaaaat ggaccggaaa tttgggcact
     5881 gcctggagca atcccggtaa ctggtcaacc tcttccatcc cagggatcaa cgatgtggtg
     5941 gtggtccctt ccgtgccatc cggaggcaac ttcccggaag tcggcagcgg cataacggca
     6001 acctgttact tcctggaact ggcacctggc gcaactctaa aagttaaaac cggcggcacc
     6061 ctgaatgttt tgaattattg atcaaactct ctttcattcc ttgttcagtt tgaccatgca
     6121 gggctttggg ccaatatttt ttgccaccaa aaaacgaata cacaaaactc cactaaagga
     6181 tttagcagac tgccaatccg cgtgaatccg ttaaatccgc gtcatccgtg tgctatttaa
     6241 ttgaaagtag attccacaaa atatcataaa taatcaaaat atcaggccat atttctaata
     6301 tatagtcgta tttttacgcc atatttttaa actatgtata aaagagatat tgaaaataca
     6361 attaaagaat ggctgccggg taaagaagtc gttattttat acgggcctcg tcagtcgggc
     6421 aaaacaacac ttctcaccat gttgttcgac caaaatcctg atgctttaat cctgaattgt
     6481 gaaaggcctg atcttgccga tatccttgag tcctctgatt tatcgaggat aaaaatgatc
     6541 ttcggggata agaaaattat tgcccttgat gaagcactta aaatccggaa tatagggcct
     6601 ctgctgaaac tggtctatga tgagccctct tttgttcaga aaataatagc gaccggatcc
     6661 agcagttttg agctggcaaa tactattagt gaaccactga ccgggagaaa tgttaaattc
     6721 ttacttcttc ctttatctat cggtgaatta tcaggagcca gaggttggct ttggatttat
     6781 gaaaatttga atctaatgcc aatatatgga tcgtatccgg gaattattga tatatcagga
     6841 gaatttaaaa caaggaaact gtttgatctg gcttcggatt acctgtttaa agacattttg
     6901 attcatgaaa atctgagaaa tccttcttta ttgaggaaac ttcttaagtt gctggctctg
     6961 caggtaggat cacaggtatc atttcatgaa ctatcaaaaa cattggctgt atcttcgaaa
     7021 actattgaaa agtatatcga cctgcttgaa aaaagctttg tcatattttc ccttccttca
     7081 ttctccagga atcaaagaac tgaaatcaga aaaagcagaa aattctactt ttatgatctg
     7141 ggtattagaa atgcgctgat aaccaatttt aatgatcccg attccagaac agataccgga
     7201 gcgatctggg aaaacttctg catcctcgaa agacttaaat ataacctgat ccatcagcca
     7261 tttgcacagc tttatttctg gcgaacatac gatggagcgg aaattgacct tgttgaagaa
     7321 gccaacggta aaataaacat ctttgaattt aaatggaggg gcaaatcaaa tgcccgcttt
     7381 cccaggtctt tctcggaaaa ctatgaaata aataccggca aactgatatc aaaggagaat
     7441 atacacgaat taattgcctg atacttgcgg gttgccaact ccggcaaatc aatgaggatg
     7501 tatctccggg cagaccttcg gggttctctc agaaaaatta accctgatac attcagccac
     7561 cgcttctttc acctcctccg gccccggatt cagctcggcc atcgtgaacg tgccgttatc
     7621 cggatcgatc cggttataaa tctgcaggta caaatggtag atgatccgca ggctgagcat
     7681 atgcctgtgg tccagccttc cttccagcga ccggatcatc ttttcggtat cggtcttata
     7741 ttttccggcc tgctctttat aatccctgat cagttccctg aaaccatccg gaatagcgct
     7801 gccccgggcg atctctctga gatctccggc agttaacccg tgcttttcca gcaggtcgag
     7861 ggcaaaatag ttcaggttgt tgaactggtc tttctggaaa tcgcgcacaa tatgcacaag
     7921 gtagctgaaa atagcacaag gcctggccac atccgagatt gaaaggttga tcggcacata
     7981 ttctccctgt tgtttatcca gacagcagag gtggacaaaa accgatgcgg gtccgttgga
     8041 agccccttcg gcgtagtcaa gaaagtcgtc gaatgtgcgg aacccgtcat ggttgatgtc
     8101 gtagaccatc gaacgggcaa agttgtaaaa gaagtgcaac gggatccgga accgacggat
     8161 cgtttcggtt acctcaccga aaaacggatc attcgtccgg tcaagcccca ggcagtcgat
     8221 ccatgcattc acctggtcgg tatagatctt cttctccatg cagctcaaca tcccgtcgag
     8281 cgccttccgg tcgtcgatca tatcatcgat gtagcgcatg aaacggtaga tggttgccgc
     8341 cgcttcgtag cgttcatctt cccagaaacg cgctgcaatc agaatgttgg ggtggtcttt
     8401 gatcttgtga aaatcaatct tactaaaggt ttcagcaata atggtttcca gattattcat
     8461 agttattatt caatttcacc gttcaccgtt caccgttcac tccaattatt ttctccgctg
     8521 tcagtcttgc cgaaatcagc gccaaaggta caccgtttcc cggatgggtg cttcccccgg
     8581 caaagaaaag attcttatat ttattgtgtt gattatgagg acgaaaatag cccatctgca
     8641 tcaggttgtg atttaaactg ccgaaggtgg cacccttgct gaggttgaac atattcttcc
     8701 agatgacggg attgtaaacc ttctcgaatt tgatatgttt ctcaaaatcc gcaaatcctt
     8761 ccatagccag tcttttcaat actgccgccc tcgcctcctc cttcttaacc tcccaatcaa
     8821 ttcccaaatc cccaaatttc ccattcccca tatgccccac cggcacaatc accgaaatgc
     8881 tgtcgtgccc ttccggagct gccgatttat cagcccgtgc cggggaatgc aggtaaaaac
     8941 ttggctcggg cgcaaacccg cttccgttga aaacctcttc aatattgttc ctgtaattat
     9001 ctgaaacata cacattatgc tgctcaatcc ccggtaaaac cgtgtccatc ccccagtgaa
     9061 aaacgattgc ggagcaggta tatcccagct tttttatccg attggcctga ggacctgaag
     9121 gcaaaagttc ctggtaaaca tagggcagat cggcattggc cagcacaata tccgcctcat
     9181 aaaaggtacc gtcggccagt tcaatgcctg ccacccgttc gcctgcaacc cgaatccccg
     9241 acacattcgc ccggtactga atttcaacac cattcccaat agcaatcttc tccaggctct
     9301 ccgataccct gttcatcccc cctttcggaa aatatacccc atcggtcagc tccatgaaag
     9361 gcagcatcga aaaaatagcc atcgcctcaa atggattctg ccctacataa atgttctgaa
     9421 aagtaaaagc ggtccgcagg ttttcattct taaaatacct ggcagatgtc cggtaatggt
     9481 ttccgaaagc attcagccgg aacaacctga aaaggttcac cgggttgatg aattgaaaga
     9541 tgttgtcgta gttccggtcg atgatggtct ccatggaaac tttgtacgct ttaaagctct
     9601 tgtccatgta tctcagaaaa gccgtgtagc ttcccggttc catcgcctcc agctggtcct
     9661 gcatccgcat caggtcggag gtgaatttca gctccgaccc atcggcataa cgaaggcggt
     9721 acaccggatc catcctgatc agatccagct cgtcgcgcat gtttctcccg aaatcggcgt
     9781 acatccgttc gtacacctgc ggcatcatca gcaaagtggc tccgatgtcg aagcggtgac
     9841 cctctttttc gaaactggca caccgcccgc ccggaaaagc gttcttttca aatattgtta
     9901 ccctgaagcc cttaatggcc aaatagttag cggcagacag gcctgccgcc cccgcgccta
     9961 tgatcgctac tgttttcata ttcgcaaagg acaggaaaaa agcgaaaggt tacaaaaatg
    10021 gcagagtttt tatttcacgc tgattttcgc agattgcagc gcaaatgaac gcagatataa
    10081 atttgcggga atccgcgaaa aattagcatt aatttgcgag aaaacactat ttttacattt
    10141 aatattattc aatatgaaaa ataacctcat tctactcgtc ctgctgtctc tcagcacctt
    10201 cctttccgca caggaaatca aaacaaaaga acctatcaaa cctgcccttc tggtcatcga
    10261 tatccagaat aagttcctcc ccatgatgga tgcaaaagac cgggaaatgg gcatgcttta
    10321 catcaaaggt tatatcgata tcttccgccg gcataacctg cccgtcatct gcatccacca
    10381 taccgatccg aaagaaggac cctttccggg caccccggag tttgcctttc cggatgatat
    10441 cggtattgcg aaagacgatc cggtggtcgt gaaaacctac gcgaatggct ttaccaaaac
    10501 cgatctggat aaggttttga aagagaaagg ggtaaatacg ctgttcctct gcggtctcag
    10561 ctcagtcggt tgcgtgctgg caacctatat cggggccacc gatcacgact ataaggcatt
    10621 ccttctgaaa gatgctatat tcagccataa atccgaatac accgaccagg tcgaaacgat
    10681 cttcgacgcc gtgggctggg atgttgtaaa actggcgatt gaatgattct tcctgaatac
    10741 tcttcgtgtt cattcgttat attcgtttaa ttcgtgttcg aaaaattcag tggaataata
    10801 aaatcgctaa ttttcactct gattattaca ttttataaca tcatgaaatc caatttctta
    10861 aaattactga gtctcctctt ctccccgtct ccatttctcc ttctctcctt gttcccactt
    10921 ctcctcttcg cccagactcc ccttccttct aactgggaca gcatcgtagc ctcaccctgg
    10981 tacaccggaa atgttagcaa tataccggct ttctgctatc ttaatcccga agatcccgat
    11041 acggggtatt ttttcattgc cgataaagcg cttccccagg tgatcccggt gtctgtaaaa
    11101 tggaagaaag gtgttccgcg gtcggcggca tttacatacc aatccgataa gataaaggtt
    11161 aaattcaccg gtacagtcag tcccgacacg atcgccggca ggatccgcac ctcccgcaaa
    11221 tccgctatcc gcctgggcat cagccctgaa cttgaaattc ttttaattaa agaaattccc
    11281 caaatccccc aatctccaaa tctccaaatc cccaaatcac caaatccccc aacctctctc
    11341 ctcccgcgat acctttcgcc tatcttcaaa aaagtcacag ttaccaaaga tattgcttat
    11401 ggcgctgcgc ctggccacta tgtgagcatg ccgatcgaga aagacagcga ttacgactac
    11461 cagcagatca tcctggatgc cttgaagaat atgtgggtaa atccggggaa aaaagcgctg
    11521 ctccacctgt tcaaccgtga tccggtctcc tttgccgtat ccgacctgca agggctgaag
    11581 atggaccttt atgagcctga aggcgatact ttatccttcc ggccactcat cctcctgctc
    11641 cacggtggcg ccttcattct tggcgataaa gccaccgaat ccatcgcaga gctggcaaat
    11701 gattatgcgc gtaagggtta cgtcgttgcc tccatcaatt accgcatcgg ctttaacccg
    11761 gccagcaaga gctccctgga gcgggcagcc taccggtcgg tgcaggatgc acgggccgcc
    11821 ctccggttcc tctcgtacaa tgcagcacaa tacaaaatcg atcccggtca tatcctgctg
    11881 gggggatcca gcgccggcgc catcaccgcc ctcaatacgg cttttatgga tgaagatgaa
    11941 cggcccgaaa gcacaggcgg caacatctgg caactgcaga aagacctggg tggactggat
    12001 gaaagcggga acgacatctc cggccaatac accatcaggg ccctgctgaa cctctgggga
    12061 gccgtgaacg ataccgccat catcgacgcc ggcgagaaga ttcccgtcct gagtgtccac
    12121 ggcgacgccg ataagatcgt cccgtacggt tacaattacc cgttcctcga tatggacacc
    12181 aatgtgacca gtaacattgt
//
LOCUS       DAOQXS010000018        12187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_5692_length_12187_cov_3.573607, whole genome shotgun sequence.
ACCESSION   DAOQXS010000018 DAOQXS010000000
VERSION     DAOQXS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5692_length_12187_cov_3.573607"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..779
                     /locus_tag="PKM34_01120"
     CDS             117..779
                     /locus_tag="PKM34_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82209.1"
                     /translation="MITAWIFGGDGTFKLTFGSTLLPTAQGDNSTMIRSYPGGIIDFP
                     NQSRVRAYQLDPNGFVQVIPPNIWTPVNFTNDAPLIAGYDEQNEFTVAPAANMAVPPE
                     MAYFTALTEGYYQVNARCEFAVYMAQVMPGSYISIAIYTGPVPGATGPYAIGNNLAIA
                     TFGNMGEPVPLEWNNAPNVSDVVYLMPGQIISIWVYHNALNPLNLIQGPDKCYVSIHK
                     VS"
     gene            complement(780..1457)
                     /gene="rpe"
                     /locus_tag="PKM34_01125"
     CDS             complement(780..1457)
                     /gene="rpe"
                     /locus_tag="PKM34_01125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNQ82210.1"
                     /translation="MINFSENTFPLIAPSMLSADFGNLQRDIGMVNQSEADWFHIDVM
                     DGVFVPNISFGFPVLRHIQKHARKPLDVHLMIVDPDRYIEKFRDHGAEVISVHYEACT
                     HLHRTVGAIKKLGALAGVVLNPHSPVSLLEEIIPDVDLVMLMSVNPGFGGQSFIEGTL
                     DKIQKLKEMMALKNTTAIIEVDGGVTLENAPRLVKAGAQVMVAGQTVFGSPDPQKVIS
                     SLKKLTA"
     gene            complement(1469..2332)
                     /locus_tag="PKM34_01130"
     CDS             complement(1469..2332)
                     /locus_tag="PKM34_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNQ82211.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGRVELITAEEEVELARRIK
                     AGDKDALEKLTKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKAYARLEQQNEREPDSVEIAE
                     MLDIPESEVKESIRNSGRHISMDAPLIQDEENTLYDVLRGDDTNTPEKELMVESLRKE
                     IDRAISTLTSREADVVRLYFGLNSKHPMTLEEIGEKFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKSYLG"
     gene            complement(2402..4537)
                     /locus_tag="PKM34_01135"
     CDS             complement(2402..4537)
                     /locus_tag="PKM34_01135"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNQ82212.1"
                     /translation="MSQAIVKKFKLGDGREVSIETGRLAKQAAGAVVVKMEDTMLLAT
                     VLVAPEPMENADFFPLTVEYKEKYSATGKFPGGFFKREARPSEYEILISRLVDRALRP
                     LFPDNFYNEIQVQVMLISGDKKNLPDTLVALAASSAIMVSNIPFLGPISEARVARING
                     EFEINPPIARMEEADLDLIVAATMDNVVMVEGQMKEVSEAVMLNAIKFAHEAIKQQCQ
                     AQIELAEMVEAAREKMPVPEPVEDKELETAIHEYAYPKFYDIARQGMNNKHQRHDAFS
                     AVKEAFIGTIPEEKREELEPMISRYFEEEERNAVRNCILDDRIRLDGRGLEDIRPIWC
                     EVDYLPAAHGSAIFTRGETQALVTVTLGTKLDEQVLDGAVVEGKSDFMLHYNFPGFST
                     GEVKPIRGTSRREIGHGNLAMRAIKPMIPADDINPYTVRIVSDILESNGSSSMASVCG
                     GTLALMDAGVKIHKPVSGIAMGLITDPKSGKYAVLSDILGDEDHLGDMDFKVTGTVDG
                     ITACQMDIKIEGLSYEIMEQALEQAKRGRHHILGEMLKTIDSPRPDYKPHVPRIEKMT
                     IPKEFIGAVIGPGGKIIQEIQKDTGSTIIIEEVGDVGVIDIFSGNKESIEAAKERIRK
                     IVAIPEVGEIYNGKVKSIVAFGAFVEILPGKEGLLHISEIDWKRVTDIDQYMKVGQML
                     EVKLIGIDNSTGKLKLSRKALIPQPPQKQ"
     gene            complement(4624..4893)
                     /gene="rpsO"
                     /locus_tag="PKM34_01140"
     CDS             complement(4624..4893)
                     /gene="rpsO"
                     /locus_tag="PKM34_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNQ82213.1"
                     /translation="MYLSSEKKKEFFATHGKSETDTGSPEGQIAMFTHRINYLTEHLK
                     QNKKDNVTRKSLVTLVGKRRSLLDYLKDVEIDRYRAIVKSLGIRK"
     gene            complement(5019..6284)
                     /locus_tag="PKM34_01145"
     CDS             complement(5019..6284)
                     /locus_tag="PKM34_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82214.1"
                     /translation="MKFAIEESVKIRQNQCHPCSVSGTQIKTDATNLHGWLRLWPALV
                     FISLTVNVSSQQSERNWRLNGHLQSLQSFWIPPESDRWQSMTVIGNRFDFRWNPGNTI
                     SFHAGARNSLNVGQMVQTFYPYYAKMSVTEDGFFDLTRLWVSDTSYYFYSNIDRANIK
                     IRKGKFEATIGRQRINWAINMVWTPNDIFNTFNYFDFDYIERPGCDAILLEYFMNETS
                     SVQFGFKMDDEQDITSAIMYRFNRWNYDFQAFAGVMTTDIVAGAGWSGDIKGAGFTGE
                     ISYFHPRESLEDTSGILVGSVSANYTFRNSLFINASYLFNSDGTTGPAGYGNALALYA
                     DISAKKFTLAKHSVYAGIGYPVTPLIRADISAIYNPNDKSGFAGPSVDISLSNNVTFF
                     IIGQVFWGKDMTEFGDFGSLCYTRLKWNF"
     gene            complement(6268..6954)
                     /locus_tag="PKM34_01150"
     CDS             complement(6268..6954)
                     /locus_tag="PKM34_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82215.1"
                     /translation="MTTIRTINLQKVYDETKIPVNALNGITLEFHEAEFTAIVGPSGS
                     GKTTLLNMLGGLDNPTSGEVHINGTNITGLSSRKLIDFRLHNIGFVFQSFNLIPVLTA
                     RENVEFILQLQGVGRKEREQRAVELLEAVGLADKIHNRPGDLSGGQQQRVSVARALAS
                     KPRFILADEPTANLDSKSTAELLDIMEKMNRQYKTTFLFSTHDSRVMERARRIVTIED
                     GKVAKDEIRN"
     gene            complement(7041..8264)
                     /locus_tag="PKM34_01155"
     CDS             complement(7041..8264)
                     /locus_tag="PKM34_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82216.1"
                     /translation="MIIFIAWRNIWRNKLRSLVVILAVCLGLFGTLLMMAISNGMVEQ
                     KIDASIHNEISHIQVHHPDYLQEPSLSYSIDSANAIARELKAISGVKAVSGKIKAMAM
                     ASTAATAAGVTINGIDPEQENTVTQIHKYIIEGDYFETKSNSAPVVISKKLCEKLKAK
                     LHSKIIVTIQHTSGEIAYGLFRVTGIYKTANNLFDEPNIFVKKEDLAELTGFDTGKVS
                     EFTILLDESELTDPVSDAVKQKLPGLSVMSWKEIEPLLVAMSAMMDQFGYFLLIIILI
                     AMAFGIINTMTMAVLERTRELGMLMAVGMNRRKIFLMIMTETIYLSLVGTFFGIIISA
                     VTIHFTGMSGINFSAWTEGYESLGYSALVYPSLYLDFYVGMTIMVIITAIISSIYPAR
                     KALKLNPASAIREDA"
     gene            complement(8301..9524)
                     /locus_tag="PKM34_01160"
     CDS             complement(8301..9524)
                     /locus_tag="PKM34_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ82217.1"
                     /translation="MKEYFKIAWRNLWRNKRRTLLTIASVLFALLLALIVRSMHLGTW
                     NKMIESTIKSTTGYIQVHQEGYWEDKTLDNSFETSSNLEQKYRSHPNVTYIVPKLESF
                     ALLSSGNQTKGAALTGVDPDVEDLVSGLQKKIIAGKYFTADDKFIILTEGLANYLKLT
                     VGDTVVILGQGYHGVTAAAALPIGGIVKFTQPDMNNLLTYVPLSLAQEIYSAPGRLTS
                     ISFILNDPEDLDRTRNDLAALGPQNLEVMPWKDLLTELVQSFEGDNVSGLFMLGILYL
                     VVGFGILGTILMTTMERKREFGIMTAVGMRRYKLSIIIVIETLIISFIGILSGVILSL
                     PVIYYFHLNPLPLKGEAADAMAEYNMDPVMPFLLEPGFIINQSMVVMIISMLTLLYPL
                     VVINRFKIINAIKGR"
     gene            complement(9526..10296)
                     /locus_tag="PKM34_01165"
     CDS             complement(9526..10296)
                     /locus_tag="PKM34_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ82218.1"
                     /translation="MKQSFHRLYSILTILCTLLSAFPSIAQDLTAREIVKKAHDKANG
                     QTSQGTMKMTIVRPDWSREVAMKSWSKGTDYYLIYITEPVKDRGQVFMKREQDMWNWM
                     PGINRMIKLPPSMMGQSWMGSDFTNDDLVRMNSIIDDFTHTIVGSEMIERFDCHIIEL
                     IPKPEAAVVWGKIKLWISKNDYYELKGEYYDEDLVLVNTMTSSNIKKMGDRTLPSRMV
                     MVPVDKPGNQTILETIDIVFDKPIDESFFSQQNMKNIK"
     gene            complement(10366..10938)
                     /locus_tag="PKM34_01170"
     CDS             complement(10366..10938)
                     /locus_tag="PKM34_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ82219.1"
                     /translation="MPRTESQFEEIREQKTALILATALELFANEGYYPTSISKIAEKA
                     GISKGLMYNYFESKEDLILAVIGKGIRELTEHLDPDHDGFLTSEEFELFINGNFRILQ
                     ENLDYWKLYFSTMMQPPVYKLVKAKYLHFLPPLQQIVEGYFERKGVNDPQLEAVFFDS
                     LIDGIFLNYVMSPENFPLDKMKSLIINRFK"
     gene            complement(11065..11574)
                     /locus_tag="PKM34_01175"
     CDS             complement(11065..11574)
                     /locus_tag="PKM34_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82220.1"
                     /translation="MSRVLYKEIQRFNQWWIWLIILLLSGFWIWQLVQQIFMGIPFGD
                     NPAPDFVVILLGLFPLGALLLFRFMILETVIDEQGVKYRFKPFQRKPKLIKPEDVKTY
                     EVKKYNPVMEFGGWGVRYGLKGTAYNVKGNMGAQFDLKNGKRFMLGTQNPDALRYALN
                     KLFAKTDRQ"
     gene            complement(11567..>12187)
                     /locus_tag="PKM34_01180"
     CDS             complement(11567..>12187)
                     /locus_tag="PKM34_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ82221.1"
                     /translation="KYNADGIVTDIAITTVKVQNWDKTITTIPTYFLVSDSYKNWRGM
                     QESGVRRLRRHILIDLNSIKFCTPEMLERFSKYEYVSDYITQTEARLNQYNIDKGIDP
                     EVLVNGVRQTNIGVFRAYLTAYLQNNPYLHQEESLMVRQLQPTETGLPLEIYAFSKEQ
                     NWEKYEAIQSDIFDHILAGIKYFDLQLFQSPSGKDFSSTIISDFDE"
BASE COUNT         2901 a         2866 c         2953 g         3467 t
ORIGIN      
        1 gagcgctccg ggaatatttc cgcacctgtt aacgattgag accatcaccg caggggctgc
       61 cgcactcaat gcgtcacagc ttttccgcat ttctgccggt atgaatcctc catttaatga
      121 ttacagcctg gatctttggt ggtgatggca catttaaatt aactttcggg tcgacgttgt
      181 tacctacagc gcaaggcgat aattcaacga tgatccgttc ttatccgggc ggtattattg
      241 attttccgaa ccaaagcagg gtacgggctt accagctgga tccgaacggg tttgtccagg
      301 ttataccgcc gaatatttgg actccggtca actttacaaa cgatgcgcca ttgattgcgg
      361 gttatgatga gcaaaacgaa ttcaccgttg cacctgccgc aaatatggcc gttccaccgg
      421 aaatggcata ttttacagcg ctgacagaag gctactacca ggtgaatgcg cgatgcgaat
      481 tcgcagtgta tatggcgcag gttatgccgg gatcctatat atccattgca atctacaccg
      541 gtccggtgcc tggtgcaact gggccctatg caattgggaa taacctggcg atcgctacat
      601 tcggcaatat gggagaacct gttccgctgg aatggaataa tgcaccgaac gtttctgatg
      661 tggtatatct aatgcccgga caaatcattt caatctgggt ttatcataat gcattgaatc
      721 cgttgaacct gatccagggt ccggataaat gttatgtctc catacacaaa gtaagctgat
      781 caggcggtaa gcttttttaa agaggagata accttctgcg gatcaggcga tccaaagacg
      841 gtttggcccg ccaccatcac ctgcgctccg gcttttacca gccggggcgc gttttctaat
      901 gtcaccccgc catcgacctc aataattgca gtggtatttt tcagggccat catctcctta
      961 agcttctgta tcttgtcgag ggtgccctca ataaaactct gtccgccgaa tccgggattg
     1021 accgacataa gcatgaccag gtcgacgtca gggatgatct cttccagcag cgagaccggg
     1081 gagtggggat tgagcacaac ccctgccagg gcgccgagtt tttttattgc tccgacggtc
     1141 cggtgcagat gggtgcaggc ttcgtagtgc acgctgatca cctctgcccc atggtccctg
     1201 aacttctcga tgtaccggtc gggatcgacg atcatcaggt ggacatcgag cggcttgcgc
     1261 gcatgtttct gaatgtggcg gaggacaggg aatccgaaag agatattcgg aacgaaaact
     1321 ccatccatca catcaatatg gaaccagtcg gcttcacttt ggttcaccat cccgatgtcg
     1381 cgttgcaggt tgccgaaatc ggcgctcagc atcgacgggg cgatcaatgg aaaagtattt
     1441 tcagaaaagt tgatcataaa taataatgct atcccagata ggacttgaga attttgctcc
     1501 gtgacgtatg tttcagcctt cggatcgctt tttcctttat ctggcggact ctttcgcgcg
     1561 tcaggtcaaa cttttcaccg atctcttcca gcgtcatcgg gtgtttggaa ttcaatccga
     1621 aatagagcct gaccacgtca gcctcgcggg aggtcagcgt ggaaatggcc ctgtctatct
     1681 ctttccggag cgattcgacc atcagctctt tttcgggagt gttggtatcg tcgccacgca
     1741 gcacgtcata cagcgtattt tcctcgtcct ggatcagggg cgcatccatg gagatatggc
     1801 ggcctgagtt gcggatcgat tctttcacct cactttcagg gatatccagc atttcagcga
     1861 tttcgacaga atcgggttcg cgttcgttct gctgttccag ccgggcatag gctttattaa
     1921 tcttgttgat cgatccgatc ttgttgagcg gcagcctcac aatcctggat tgttcggcca
     1981 atgcctgcag gatcgattgc cgtatccacc acacggcata ggagatgaat ttaaatccgc
     2041 gggtctcatc gaaccgctgg gcggctttga tgagaccgag atttccttca ttgatcaggt
     2101 caggaaggct aagcccctgg ttctgatact gttttgaaac ggaaacgacg aaacgaagat
     2161 tggccttggt tagtttttcc agcgcgtctt tatcaccggc tttaatgcgc cgggctaatt
     2221 caacttcttc ttctgccgta ataagttcaa ccctcccgat ctcctgcaaa tacttgtcga
     2281 gtgatgcagt ttcccggttg gtaacttgtt tggtaatttt taactggcgc atagttcggt
     2341 ttatgggagt acaaagctaa cgtaatttgt acgatccccg cgcagttaag tttcaaaaaa
     2401 attactgctt ctggggcggt tgcggtatca gcgcttttct cgacagtttg agcttaccgg
     2461 tgctgttatc tatgccaatc agcttcactt ccagcatctg cccgaccttc atgtactggt
     2521 cgatgtcggt tacccgtttc cagtcaattt cagaaatatg caacagacct tctttgcccg
     2581 ggaggatttc gacaaaagct ccgaaagcga caatgctttt cactttacca ttatagattt
     2641 caccgacttc cgggatggcg acgattttcc tgatccgctc tttggctgcc tcgatcgatt
     2701 ctttgttacc ggagaagata tcgatgaccc cgacatcgcc cacctcttcg atgatgatgg
     2761 tcgaaccggt gtctttctgg atctcctgga tgatcttgcc gccgggtccg atcacagcgc
     2821 cgataaattc cttgggaatg gtcatcttct cgatcctggg cacatgcggt ttgtaatccg
     2881 gccttgggct gtcgatggtt ttcagcattt cgccaaggat gtgatggcgg ccgcgtttag
     2941 cctgctcaag ggcctgctcc atgatttcat aggacaggcc ttcgatcttg atatccatct
     3001 ggcaggcggt gatcccatcg accgtacccg ttactttgaa atccatatcg cccaggtggt
     3061 cttcatcgcc gaggatatcc gataatacgg catacttgcc gcttttcggg tcggtgataa
     3121 ggcccatggc gattccggag accggtttgt ggattttcac gccggcatcc ataagggcca
     3181 gcgttccgcc gcaaaccgaa gccattgatg atgatccgtt ggattccagt atatccgaca
     3241 caatacgcac tgtgtacgga ttgatgtcgt cggccgggat catgggtttg atggctcgca
     3301 tagccaggtt gccatgtccg atctcgcgac ggctggttcc ccggataggc tttacttcac
     3361 cggttgaaaa gcccgggaaa ttgtaatgca gcataaagtc gcttttgcct tcgacaacgg
     3421 cgccgtcaag cacttgttcg tcgagttttg tgccgagggt aacggtcacc agggcctggg
     3481 tctctccgcg ggtgaatata gcggagccgt gggccgccgg caggtaatcc acctcgcacc
     3541 agattgggcg gatgtcttcc agtcctctgc catcgagcct gatccggtcg tcgagaatgc
     3601 aattcctgac ggcattccgt tcttcctcct caaaatagcg gctgatcatc ggttcaagtt
     3661 cttcccgttt ctcttcgggt attgttccga taaatgcttc ttttacagca gaaaatgcat
     3721 catgccgctg atgtttgttg ttcatcccct ggcgggcgat atcatagaat ttgggataag
     3781 catattcatg aatagctgtc tccagttctt tatcctcaac gggttcgggc accggcattt
     3841 tttccctggc tgcttcgacc atttccgcca gttcaatttg agcctggcat tgctgtttga
     3901 tggcttcatg ggcgaattta atggcattga gcatgacggc ttccgagact tctttcatct
     3961 ggccttctac catcactaca ttatccatgg tagcagcgac gataaggtcg aggtcggctt
     4021 cttccatccg tgcgatagga gggttgatct caaattcacc gttaatgcgg gcaacgcggg
     4081 cttccgagat aggtcccagg aaggggatat tggaaaccat aatggcgctg gaggcggcca
     4141 gtgcaacgag ggtgtcgggc aggttcttct tatcgccgga gatcagcata acctgcacct
     4201 ggatctcatt gtagaaatta tcggggaaca ggggcctgag cgcgcggtcg accaggcggg
     4261 agatcagaat ttcatattca gatggacggg cttctctttt gaagaaaccg ccggggaatt
     4321 ttcctgtggc agagtatttc tctttgtatt ctacggtgag cgggaaaaaa tccgcatttt
     4381 ccataggttc gggtgcaaca aggactgtag ccaggagcat ggtgtcttcc atttttacga
     4441 caacagcacc tgcagcttgt ttggccagac ggccggtttc gatggagacc tcacgtccgt
     4501 ctcccagttt gaatttttta acgattgctt gtgacatttt tctcttcttt ttttccgatt
     4561 gataaataca aaaaaaggca atgctgagat tgcctttttg gtctatatta ttgacaataa
     4621 tatttacttt ctgatgccga gacttttgac gatggcccgg tacctgtcga tttccacatc
     4681 tttcaggtaa tcgagcaggc tgcggcgttt accaacgagc gttaccagtg atttacgggt
     4741 gacattgtct ttcttgttct gtttcaggtg ctcggtcagg taattgatcc ggtgcgtgaa
     4801 cattgcgatc tggccttccg gtgaacccgt atccgtttcc gatttcccgt gagttgcaaa
     4861 aaactctttt ttcttttctg acgagagata catatctatc ctggtattaa ttttttaatt
     4921 tgagcgtgca aagataggaa aataatttag ttagacaact gcagggggat atttttttag
     4981 ttgttttaaa gaaacattac ccaggaacca gacattctct aaaagttcca tttcagccgg
     5041 gtatagcaaa gggatccgaa atcgccgaat tctgtcatat ccttccccca gaagacctga
     5101 cctataatga aaaaagtgac gttattcgac agggagatgt cgaccgatgg accggcaaat
     5161 cccgatttat cgttcggatt gtaaattgct gagatatcgg cccggatcag tggggtaaca
     5221 gggtatccta tgccggcata aacggaatgt ttcgccagcg tgaacttttt ggccgagata
     5281 tcggcataaa gggcaagtgc attgccataa cccgcaggtc cggtcgtccc gtcgctgtta
     5341 aacagatatg aagcattgat gaacagcgaa ttcctgaaag tgtaattcgc gctgactgaa
     5401 ccgactaaaa ttccgctggt gtcttccagg ctttcccggg gatggaaata gctgatttca
     5461 ccggtaaaac cagctccttt aatatcgccc gaccatccgg cccctgcgac gatatccgtt
     5521 gtcatgactc ctgcaaaggc ctggaaatca tagttccacc tgttgaaccg gtacatgatg
     5581 gcggatgtga tatcttgctc atcatccatt ttaaaaccga actgaacgga agaagtttca
     5641 ttcataaaat actccagcag gatcgcatcg cacccgggcc tttcaatata atcgaaatca
     5701 aaatagttga aggtgttgaa aatatcattc ggcgtccaga ccatattgat ggcccagttg
     5761 atacgctgcc gtccgatcgt cgcttcaaac ttgcctttcc tgatttttat attggcccgg
     5821 tcgatattcg aataaaaata gtacgaggtg tcgctgaccc acagccgggt cagatcgaag
     5881 aacccatctt cggtaaccga catcttagca taatagggat aaaaggtctg gaccatctga
     5941 ccgacattga gactgttcct ggctcctgca tggaaagaga ttgtgttccc gggattccat
     6001 cgaaaatcga accggttccc gatcaccgtc atcgattgcc aacggtcgga ttcaggcggt
     6061 atccagaaac tttgcaacga ttgcaggtgc ccgttcagcc gccagttcct ttcactttgc
     6121 tgcgatgaaa cattgactgt taatgatatg aagaccaggg cgggccataa tcttaaccat
     6181 ccgtgaagat tcgttgcatc cgtttttatt tgcgttccac taaccgaaca cggatgacac
     6241 tgattttgac ggattttcac cgattcttca attgcgaatt tcatcttttg caacttttcc
     6301 atcttcaatg gttacaattc tccttgccct ttccataacc ctcgaatcgt gggtagagaa
     6361 caaaaaagtg gttttgtact gccggttcat tttttccatg atatccagca gttccgccgt
     6421 gcttttactg tcgaggttgg cggtgggttc atcggccagg ataaaccggg gttttgaagc
     6481 gagagccctg gccaccgaaa cgcgttgctg ctggccaccc gaaaggtcgc cgggccggtt
     6541 gtggattttg tcggccagac ctaccgcttc gagcagctcc acggcccttt gttcgcgttc
     6601 tttcctgccg actccctgca gctgcaggat aaattccaca ttctcgcggg cggtcagtac
     6661 cgggataagg ttgaacgact ggaagacgaa accgatgttg tgcaaccgga aatcgataag
     6721 ttttcgtgaa gacagaccgg taatgttggt gccgttgata tgcacctctc cgctggtcgg
     6781 attgtcgagc ccacccagca tgttcagcag cgtcgttttt cccgatcccg acggcccgac
     6841 tatcgctgta aattcagctt catgaaactc cagcgtgatc ccgtttaaag cattgacagg
     6901 gattttggtt tcatcgtaaa ccttttgcag attgattgtt ctgatcgttg tcattttgcg
     6961 ggttgtaagg ttgttaaggg ttgtaagttt aacatatcac ttgttcataa tttcaaaata
     7021 tcacaatatc aagaatcctt tcatgcgtct tcacggatgg cggatgcggg attcagcttc
     7081 agcgctttcc gtgcagggta tatcgaagag ataatcgctg taatgatcac cattatcgtc
     7141 attccgacat aaaaatcaag ataaagtgaa ggatagacca gggcactgta gcccagcgac
     7201 tcatatcctt cagtccatgc agagaaattg atgccgctca ttcctgtgaa gtgtattgta
     7261 acggcactga tgataatacc aaagaatgta cccacgagcg aaaggtaaat cgtttcggtc
     7321 atgatcatga ggaagatttt tctcctgttc attcccactg ccataagcat tccgagttcc
     7381 cgggtccttt ccagcacggc catggtcatg gtattgatga ttccgaaagc catggcgatg
     7441 agtatgatga tcagcaggaa gtacccgaac tgatccatca ttgctgacat ggcaacgagc
     7501 aacggttcga tttctttcca tgacatcacg ctgagacctg gcagcttttg cttgactgcg
     7561 tccgacacgg gatcggtcaa ttctgattca tcgaggagga ttgtgaattc gcttaccttt
     7621 ccggtgtcaa atccggtgag ttctgccagg tcttcctttt tcacaaagat gttgggttca
     7681 tcgaacaggt tattggcagt tttataaatt ccggtgaccc tgaagaggcc ataggcaatc
     7741 tcacccgacg tatgctggat cgtaacgatg atcttggaat ggagttttgc tttcagtttc
     7801 tcacaaagtt ttttgctgat aacgacggga gcgctgttgc ttttggtttc aaaatagtcg
     7861 ccttcaatta tgtatttgtg tatctgggtt accgtatttt cctgttcggg atcaattccg
     7921 tttattgtca caccggcagc cgtggctgca gtgctggcca tagccatggc cttgatcttg
     7981 ccggatactg cctttacgcc gctgatggct tttaactccc gtgctattgc attggctgaa
     8041 tcaatggaat aggataatga gggttcctga agataatcag gatgatgcac ctggatatgt
     8101 gaaatctcgt tatggattga cgcgtcgatc ttttgctcca ccatgccatt ggaaatagcc
     8161 atcatcagca gcgtgccgaa caagccaagg cataccgcaa gtatgactac caggcttctg
     8221 agcttatttc tccagatatt gcgccatgcg atgaaaatta tcataatgac aaatattaaa
     8281 agttatgttc agatttgatt ttatcttcct ttaatggcat tgattatttt gaaccggttg
     8341 ataaccacga gcggatacaa cagcgtcagc atggaaatta tcattaccac catcgactgg
     8401 ttgataatga atccgggttc gagcaggaaa ggcatgaccg gatccatgtt gtattctgcc
     8461 atggcatcgg cggcctcgcc tttaagcggt agcggattca gatggaaata gtaaattacc
     8521 ggcagactga gaataacacc ggatagtatg ccaatgaagc tgatgatcaa ggtttcaatg
     8581 acaataatga tagatagttt gtatcgccgc atccctactg cggtcatgat gccgaattca
     8641 cgtttgcgtt ccatagtggt catcagaatg gtgcccagta tgccaaatcc tacgacaaga
     8701 taaaggatac cgagcataaa cagaccgctg acgttatccc cctcgaaact ctgcaccagt
     8761 tccgtgagca aatctttcca gggcatgacc tccaggtttt ggggccctaa agccgcaagg
     8821 tcgttccggg tcctgtcgag atcttccgga tcattgagta tgaaagagat cgatgtcagg
     8881 cgtcctggcg cagaatagat ctcctgagcc agggagagag ggacatatgt taacaggtta
     8941 ttcatatcgg gctgagtgaa tttgacgatg ccaccaatcg gcaatgcggc agcagcagtc
     9001 acaccgtggt aaccttgccc gagaatgaca accgtatctc caacggtcag tttcaggtaa
     9061 ttggcaaggc cttcggtcag gatgataaat ttatcgtcag cagtgaagta tttaccggcg
     9121 atgatctttt tctgtaggcc cgataccaaa tcttccacat cgggatcgac tccggtaagg
     9181 gcagcacctt tggtctgatt cccggaagaa agcagcgcaa acgattcgag cttcggtaca
     9241 atataagtca cattgggatg gctcctgtac ttctgttcga ggttgctgct ggtttcgaag
     9301 gaattgtcga gggttttatc ctcccagtat ccttcctggt gaacctggat atatcccgtt
     9361 gtacttttaa tggtactttc gatcatttta ttccaggtgc ccagatgcat cgaacgtacg
     9421 atcagcgcaa ggagcaaagc gaataatacc gatgcaattg tgagcagggt cctgcgtttg
     9481 ttgcgccaca ggtttctcca tgctatctta aaatattctt tcatcttatt ttatattctt
     9541 catattttgc tgagaaaaga aactttcatc gataggtttg tcgaaaacga tatcgatcgt
     9601 ctcaagaatg gtctggttac ccggtttatc gacaggaacc atgaccattc ttgaaggaag
     9661 ggttcggtcg cccatttttt taatattgga agatgtcatg gtattgacca ggaccaggtc
     9721 ttcatcataa tattcccctt ttaactcata ataatcattc ttggatatcc atagtttgat
     9781 tttgccccag accaccgcgg cttccggttt ggggattagc tcaatgatat ggcaatcaaa
     9841 tctttcgatc atttctgatc caacgatagt atgtgtaaaa tcatcgatga tcgagttcat
     9901 cctgaccagg tcgtcgttcg tgaaatcgga acccatccac gattgcccca tcatcgaagg
     9961 ggggagcttg atcatccggt tgatgccggg catccagttc cacatatcct gttcccgttt
    10021 cataaatacc tgcccccggt ctttcaccgg ttcagtgatg tagatcaggt aataatcggt
    10081 ccctttcgac caggatttca tagcgacctc cctcgaccag tcgggccgta cgatggtcat
    10141 tttcatggta ccctggctgg tctggccgtt ggctttgtcg tgcgcttttt taacgatttc
    10201 gcgtgcggtg agatcctggg caatggaagg aaaggccgag aggagagtac ataagatcgt
    10261 caggattgaa tagagccggt ggaaagactg tttcataaaa gtaggtgttt aaattttaat
    10321 tcttaattct taattcttaa attttaatgt attgtattgg gatgattatt taaaacgatt
    10381 gatgataagg gatttcattt tatcaagggg aaaattttcg ggggacatga catagttcag
    10441 gaagatgccg tcgatcaggc tgtcgaaaaa tactgcttcc agttggggat cattaacacc
    10501 tttacgttca aagtaccctt caacgatttg ttgaagaggg ggcagaaagt ggaggtattt
    10561 ggctttgacc agcttataaa caggcggttg catcatggtc gaaaaataca gtttccaata
    10621 gtccagattt tcctgcaaaa tgcgaaaatt cccgttaata aagagctcaa attcttcgga
    10681 cgttaaaaaa ccatcatggt cggggtcgag atgttcggtg agttcccgga tccccttacc
    10741 gataacggcg aggataaggt cttccttgct ttcgaaataa ttgtacatca gccctttgga
    10801 tatccctgct ttctctgcaa ttttgctgat ggaggtggga tagtatcctt cattggcgaa
    10861 caactcgagc gctgttgcca ggatcagggc cgttttctgt tccctgattt cttcgaattg
    10921 agattctgtt cgaggcatac tgttttatga ttgaacggtc ggtcaaaaat aaaatgtttt
    10981 ttttgctgac gcaagaaaaa attaatttaa tttaccaaag gtaatttgtg gttaataaaa
    11041 agttaaactg gaccaatcga tggtttactg gcggtcagtt tttgcgaaca gcttgttcaa
    11101 tgcgtaccgg agtgcatcgg gattttgtgt tcccagcatg aaccgttttc cgttcttaag
    11161 gtcgaactgg gcgcccatat ttccttttac attgtaagcg gtccctttca agccataacg
    11221 gactccccat ccgccgaact ccattaccgg gttgtacttt tttacttcat aagtttttac
    11281 atcttccggt ttgatcagtt tcggttttcg ttgaaacggt ttgaaccggt atttgacccc
    11341 ttgttcgtcg ataaccgttt caaggatcat aaagcggaac aaaagcaacg cccctaacgg
    11401 gaagagtccg aggaggatta ccacgaaatc gggcgcgggg ttatcgccaa aaggaatacc
    11461 cataaagatc tgctgaacga gctgccagat ccagaatccg cttaataaca gtatgatcag
    11521 ccatatccac cattggttaa aacgctgaat ttctttgtat aacactctac tcatcgaaat
    11581 ctgaaattat tgttgatgaa aaatctttcc cggaagggct ttggaacaat tgcaggtcga
    11641 aatactttat accggccagg atgtgatcga aaatatcgga ttgaatggct tcgtattttt
    11701 cccagttctg ttctttgcta aaggcataga tttcaagcgg cagacctgtt tcggtaggct
    11761 gcagctgacg taccatcaac gactcttcct ggtgaaggta ggggttattc tgaaggtaag
    11821 ccgtcaggta ggccctgaat accccgatat tggtttgccg gacgccgttg accagcacct
    11881 ccggatcgat acctttatct atattgtatt gattcagtct ggcttcggtt tgggtgatat
    11941 agtcgctgac atattcatat tttgagaatc gctccagcat ttccggcgtg cagaatttaa
    12001 tgctgttcag gtcgatcagg atatgccggc ggaggcgccg cactcccgat tcctgcatgc
    12061 cccgccagtt tttataggaa tcgctgacca ggaaataggt tgggatggta gtgattgttt
    12121 tgtcccagtt ctggactttc acggttgtaa tggcgatatc ggtcacgatc ccgtcggcat
    12181 tatattt
//
LOCUS       DAOQXS010000019        11597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6015_length_11597_cov_4.252989, whole genome shotgun sequence.
ACCESSION   DAOQXS010000019 DAOQXS010000000
VERSION     DAOQXS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6015_length_11597_cov_4.252989"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /locus_tag="PKM34_01185"
     CDS             <1..2475
                     /locus_tag="PKM34_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNQ82222.1"
                     /translation="KDAASAAIYGARAANGVVLITTKTGTAGKMSLNFSTYYGIQNVG
                     KKLEMLDADEYRMIWNEAARNSNRAEPFDLAEVPEANTDWQEYLFTKNAPVSNYELSI
                     AGGNEKTTYASSVSYFSQQGIIGGEKSQFDRITARLNGQVKATNWFTFGSDLAYSSLT
                     RRGISSNGSFNTAYSSALNLDPLTPLYETNPDTLSLPPFSEQPVVKTPDGKVYGISDY
                     LDAEVVNPIALLEIQTGETRVDKFVGNIFGEITFMEGLKFRSTLGTDLAYVHWDTYGP
                     LYFLNGSQSNTDKTNVSKSMDRYFNWQWENTLTYTKKIKDHSFSLLAGTSAKEDNYEN
                     LSGFNADVPITDPDNVYLNMATDTSWRANGGARDYALFSVIGRVTYDFKSRYAFTGII
                     RRDGSSNFGPNNRYGWFPSVGVSWLLTEEKFIPELGPVDFLKLRASWGINGNDRIGYY
                     QYVSTIDKSRGYIFGGATNTGASPGYIENADIKWEESEQIDIALDAGLFNNRLTATFD
                     YYIKNTIDLLETIEIPGYVGNNPPVANVGSVRNEGVEMSINWRHYKEKIRYYAGVNAA
                     FNKNKMTEIANEQGFLTGATWAVAGFVTRCEEELPIAYFWGYKTDGIFQNENEVFQHY
                     GTDGVPLQPNAKPGDVRFIDVNGDNKINEEDRTMIGNPTPDWTLGINGGIEYMNFDLS
                     FLFTGAFGHQIFNGAQRQDLFFTNRTTAILDRWTGEGTSDDIPRMAWVDVNNNYRVSD
                     LYIEDGDFLRLKNLQIGYTLPKKILDRIKSGAWRFYISAENLVTFTKYTGADPEIGAI
                     SSLDIGIDRGMYPSAVTFRFGTSISF"
     gene            2494..4050
                     /locus_tag="PKM34_01190"
     CDS             2494..4050
                     /locus_tag="PKM34_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNQ82223.1"
                     /translation="MKKSIIYPISIIVLVMLTIGCSKDFLDRQPLDREVSTNFYRTEA
                     DAMMALAAAYDALGWDQMPGVSWAPFCTVSDILSDDSYAGGADANDGMDENELNNFTI
                     PTTNLLVKATWMKNYVGIYRANLLMEKMDQITMADEKKARMIAECKFLRAYYHFDLVR
                     LFENIPLLTATIKGPSEYNQDQETPKEVYDQIALDLVEAAAVLPATIPADESGRVSKW
                     AAQSLLGRVYLFYNGVYSADLVAGDMTIDRAKALEYLEDVITNSGHDLFPEYSTNFRL
                     AGEYGIESVFEISHGDTPAWWDWNYVVGGEGNLAAQMQGPRVTNSSNWDRGWSFGVVS
                     QKLVDDFGDDPRLAFTIVKEEELDGTLTKGFQHTGYYSRKYSSDAEHWGSDGQFELNR
                     TCNFRVIRFSDVLLMAAELGSPNAQNYLDRVRSRVNLGSIPATPENIYRERRLELSLE
                     GIRYFDVLRKGIAFASQEFTVVDVRGPKYTDEQVVYDHTFNSATKGFLPIPQTEIDMS
                     AGVLKQNEGY"
     gene            complement(4284..4808)
                     /locus_tag="PKM34_01195"
     CDS             complement(4284..4808)
                     /locus_tag="PKM34_01195"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HNQ82224.1"
                     /translation="MQNISLISYGYFDKALLDRIAENISQEFQSQVELREGHLDLSLY
                     YDPGRRQYNGTRLLEVVDKLPLESSYKILALFNVDLFIPILTYIFGQAYLGGKSGIVS
                     SYRLRNELYGLKQDDKLLAGRIIKVCLHELGHMHGLVHCSQPQCIMRSSTYVEDIDLK
                     ERYFCGECNAKLCE"
     gene            complement(4825..6180)
                     /locus_tag="PKM34_01200"
     CDS             complement(4825..6180)
                     /locus_tag="PKM34_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNQ82225.1"
                     /translation="MKEKIMTGKISILIVDDEESVRDSLYNWFIEDGYHVECAANAKE
                     ALNLIEQKEFSIVLADLKMPGMDGLEMQRRIKTLNPEAIVIIMTAFATVDSAVRALKD
                     GAFDYITKPFDPDDLSHLIRNASKQIMLKAENDALRDKIVSLENIDDIIGTSPAILQV
                     LKEVESVAQSNASVIITGESGTGKELIARAIHANSSRRYFPLVSVHCGALTESLLESE
                     LFGHEKGAFTGAMYNRKGRFEMADGGTIFLDEIATVSMKMQIELLRVLETRSFMRVGG
                     NREISSDFRVICATNRDLKAMVGNGTFREDLYYRLNVVGIHVPPLRERPEDIPLLAEY
                     FIKKYCRSMSRDVIPIEPAALKRLQEFDFPGNVRELENMIERAIVVGNGKEIRVKDLP
                     LGREVSTASTAESLEEQEKKHIVQILTRYDWNISRAAKALQIDRVTLYNKIRKYDLNQ
                     E"
     gene            complement(6155..7732)
                     /locus_tag="PKM34_01205"
     CDS             complement(6155..7732)
                     /locus_tag="PKM34_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82226.1"
                     /translation="MSVILFASFSIIFRSVNHKYLNSVIVQNGHNVGSIVKGALYQSM
                     LANDETSLQQTLDAIHSISGIDEVNLYNNRNDLIYSVFSSEIADRGNPDCITCHEDFS
                     GMFSKTEISYKIIDKKSLCTMNQTIKGYRQLLISSPILNEKTCYTADCHAHQANETFL
                     GSLLIKVPLTELDSTVSKSSARFVFFSILSTLIMATILIIFTRKRIKKPLNEIILASE
                     AVGKGNTSVRLEIDKNELDDIKVVSKAFNDMLDKIQASTKELQNWSHQLEYKIQKKSE
                     ELLEAQNELIQIEKIASLGKLSSSVAHEINNPLAGVLTYTKLVSKQLGKADMDENARQ
                     SIQKHLKVIETETKRCGDIVKGLLDFSRKDQENFSNRHLNEVLQETHNLMAHQMKVEN
                     IQFTTDFAASNDVVSCVENQIKQACIAILVNASEAVSENGDVAVRTFNPDGEHIRIEI
                     TDNGTGIAPDDIPHIFEPFFSAKNKSSGIGLGLSIAHGIVLNHKGRIEVDSRLGSGTK
                     ISIILPLANLEGKDHDR"
     gene            complement(7912..8631)
                     /locus_tag="PKM34_01210"
     CDS             complement(7912..8631)
                     /locus_tag="PKM34_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82227.1"
                     /translation="MDGFTYHDIFQTKGIEYIIIILFLLLLIPFWIIINKNDYLKTKI
                     KKAVRFLTRDTLRVPRGVFISPMHTWAFMEKSGMAKIGPDDLLALLAGEVNLEFLKKP
                     GEEVKRGDLIAEVGVGEKKLQIISPVSGTISRPNHSLAEQRHLFADDPYGQGWIYEIS
                     PSAWKEETNSYRLGEEAVSWTYGELDRFKDFLAKSMVRHSLTGKGIILQEGGEIMAGS
                     LAEMPEQIWVEFQEEFLSDHH"
     gene            complement(8668..9906)
                     /gene="nrfD"
                     /locus_tag="PKM34_01215"
     CDS             complement(8668..9906)
                     /gene="nrfD"
                     /locus_tag="PKM34_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNQ82228.1"
                     /translation="MKRAMIKGEYTLEADRQKEGFWKFILKEMKPRGKIFTPFNIISF
                     PIMLTGLVLIVIRFWKGIGSVTNLTQEVPWGLWIGFDVVTGVAFAGGAYVLTFMVYIL
                     NMKKYHSIVRVTVLNGFLAYVFYAGALLLDLGRPWNVINPIIGNDFGYSSVLFLVAWH
                     FLLYMLAQLIEFSPAIAEWLGARRAHRILSGMTIAAVIFGITLSTLHQSGLGALYLMA
                     KEKIHPLWYNEFIPIMFFVSSIFAGLSLVIFEGSISHKVFFHQISEPNRKAHHGIVIG
                     LSKICAVAMFAYFFLQILIFVHGQRWEYLGTPMGYWYLVEMIGFVLFPMLLFFTSYRR
                     HNALLVKIAAIITILGIIINRLNVTVIGFKWNLEPHYFPSWMEIVVTLAVIFTEIWIF
                     RWIINRMPVLRDSPEWAKNH"
     gene            complement(9887..10762)
                     /locus_tag="PKM34_01220"
     CDS             complement(9887..10762)
                     /locus_tag="PKM34_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ82229.1"
                     /translation="MPIDRRNFFKVLGVTGVALATGKKLNAATTEAAKDAPPEEFMGV
                     LYDASRCVGCQSCEYACAAAHNLPEPADSPEAGIIRKASETQQNVINLYNTSKGEVYV
                     KQQCMHCNSPACASACLTNAMHKTHTGPVIWRGNKCMGCRYCMVSCPFDIPKFEYHSP
                     NPEIRKCDMCFDRLKEGLMPACVTECPAEALMFGTRREMIKEARRRIHDNPDVYYDHI
                     YGEHEAGGTGFLYLSPVPFEELGFNTSIQKSSYPELSKGFLYSVPTIFVLWPAILLGI
                     REATKDKPLNEESHD"
     gene            complement(10806..>11597)
                     /locus_tag="PKM34_01225"
     CDS             complement(10806..>11597)
                     /locus_tag="PKM34_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82230.1"
                     /translation="AGFSGLVAGERLAKAERLINRQSPDDPSGNIFHSNVALIVILSF
                     LFVALLVSALLFFAKKGGVQPSPEKIARGQLNPESIGIPQGLLFSKSHTWSFMDRDGK
                     VKIGIDDFMQHIVGPVTRVEMKKAGEKIRKGDILVKLVQNGKQLHLYSPVSGTIAEFN
                     PLLKSDPSQLNASPYTSGWLYRVEPANWMKELQFMEMADSYRKWISDEFSRLRDFLAT
                     SIKVSRPELQYVVLQDGGHLKDHVLADFGPEVWEDFQTSFLDKAR"
BASE COUNT         2979 a         3067 c         2655 g         2896 t
ORIGIN      
        1 aaagacgccg cctcggcagc catctacggc gccagggccg ccaacggcgt cgtcctgatc
       61 accacgaaaa cagggacagc cggcaaaatg agcctcaact tctcgactta ctatggaatc
      121 cagaacgtcg ggaaaaaact cgaaatgctc gacgccgacg aataccggat gatctggaac
      181 gaagcagccc gaaacagcaa ccgtgccgaa cctttcgact tagcagaagt cccggaagcc
      241 aatacagact ggcaggaata cctgttcaca aaaaatgcac cggtcagtaa ttatgaactc
      301 agtattgccg gcggaaatga aaaaacaacc tatgcttcat ccgtttccta tttctcacag
      361 cagggtatca tcggcgggga aaaatcacaa ttcgaccgta ttacagcccg gctcaacgga
      421 caggtcaaag ccaccaactg gtttactttc ggcagcgacc tggcctattc cagcctcaca
      481 cgcaggggga tctccagtaa cgggtcgttc aatacagcct acagcagcgc ccttaacctc
      541 gatcctttaa caccgctcta tgaaaccaat cccgatacgc tcagcctgcc cccgttttca
      601 gaacagccgg tggtgaaaac cccggatggg aaagtttatg gcatatctga ttaccttgat
      661 gccgaggtag ttaatcctat tgctttactc gaaatccaga ccggagaaac ccgggttgat
      721 aaattcgtcg gaaacatttt cggtgaaatc acctttatgg aagggctgaa gttccggtca
      781 accctcggaa ccgacctggc ctatgtccat tgggatacct atggcccgct ctattttctg
      841 aacggctccc agtcaaatac cgataagacc aacgtttcca aatcgatgga ccgctatttc
      901 aactggcagt gggaaaatac cctgacctac accaaaaaga ttaaagatca ttctttctcg
      961 ctattggccg gtacttctgc taaagaagac aattatgaga acctgagcgg ttttaatgct
     1021 gacgtcccca ttaccgatcc cgataatgtt tacctcaaca tggccaccga cacctcctgg
     1081 agagccaatg gcggcgccag ggattatgcg ttgttttccg tgatcggcag ggtcacctac
     1141 gactttaaaa gcagatatgc cttcaccggg atcatccgcc gtgacggatc ctccaacttc
     1201 ggcccgaaca accgttacgg ttggttcccg tcggtaggcg tatcctggct gctgacggag
     1261 gagaaattca tccccgaact gggtcccgtt gatttcctca aactcagggc ttcatggggt
     1321 attaacggaa atgaccgtat cggttattat cagtatgttt caaccatcga taagagccgc
     1381 gggtatattt tcggaggcgc aacaaatacc ggtgcttcgc ccggatatat cgaaaatgcc
     1441 gatatcaagt gggaagaatc ggaacagatc gatatcgcac tcgacgccgg attgttcaac
     1501 aacaggctga ccgcaacgtt cgattactac attaaaaata ccatagacct gctggaaacc
     1561 attgagattc ccggatatgt gggtaacaac ccgccggttg ccaacgtggg cagcgtcagg
     1621 aacgaagggg tcgaaatgtc aatcaactgg aggcattaca aagaaaagat ccgctactat
     1681 gccggggtca atgcagcctt taacaagaat aaaatgacgg aaatcgccaa tgagcaggga
     1741 ttccttactg gagctacctg ggcggtagcc ggatttgtca cccgctgcga agaagagctg
     1801 ccgattgcct atttctgggg ctataaaacc gacggcattt tccagaatga aaatgaagtc
     1861 ttccagcatt acggtaccga cggcgttccg ctgcaaccca atgcaaagcc gggcgacgtc
     1921 aggttcattg atgtcaatgg cgataataaa atcaacgagg aagaccgtac gatgatcggt
     1981 aatcccaccc ctgactggac gctgggaatc aatggtggca tcgagtatat gaacttcgac
     2041 ctctccttcc tgtttaccgg cgctttcggc caccagatct ttaacggtgc ccagcgccag
     2101 gatttatttt ttaccaatcg tacaacggct atcctcgaca ggtggaccgg tgaagggaca
     2161 tccgatgata ttccacgcat ggcctgggtt gacgtcaata ataattacag ggtctccgac
     2221 ctctatattg aggatggaga tttcctgcgc ctgaagaacc tccagatcgg gtataccctc
     2281 cccaaaaaga tcctggaccg tataaaaagc ggcgcctgga gattttatat ctctgctgaa
     2341 aaccttgtca cttttaccaa atacaccggt gcagatcccg aaattggcgc gatcagctcc
     2401 ctggatatcg gtatcgaccg gggcatgtac ccatcggccg tgactttcag attcggaacc
     2461 agtatttctt tctaatctta aaaacaagga aaaatgaaaa aatcaataat atacccgatt
     2521 tcaatcatag tactggtcat gctgacaatt ggatgcagca aggattttct tgaccgtcag
     2581 ccgctcgacc gggaagtcag cacgaacttt taccggaccg aagccgatgc catgatggca
     2641 ctggccgcag catacgacgc acttggatgg gaccagatgc ccggcgtttc atgggccccc
     2701 ttctgcaccg tttccgacat cctttccgat gattcttacg ccggtggcgc agacgctaac
     2761 gacggcatgg atgaaaacga gctgaataac tttaccattc caacgaccaa cctgctggtc
     2821 aaggcaacgt ggatgaagaa ctacgtggga atttacaggg ctaacctgct gatggaaaaa
     2881 atggaccaga tcacgatggc cgacgaaaag aaagcccgga tgatcgccga atgcaagttc
     2941 ctgcgggctt actaccattt cgacctggtc aggctctttg agaatatccc gctgctgacc
     3001 gctacgatca aaggcccgtc ggaatacaac caggaccagg aaacaccgaa agaagtctat
     3061 gaccagatcg ccctcgacct cgtcgaagcg gcggcagtcc ttcccgcaac catcccggcc
     3121 gatgagtccg gcagggtgag caaatgggca gcccagtcgc ttctcggcag ggtttacctg
     3181 ttctataacg gtgtttacag tgccgacctg gtggccggcg atatgacaat cgaccgggcc
     3241 aaggcgctcg aatatctcga agatgtcatt accaacagcg gccacgacct cttcccggaa
     3301 tattctacca atttcagact ggccggcgaa tatggcatcg aatcggtgtt tgagatctcc
     3361 cacggcgata ccccggcatg gtgggactgg aattatgttg tcggaggtga aggcaacctg
     3421 gctgcccaga tgcagggccc tcgcgttacc aattcttcca actgggaccg cggctggagc
     3481 ttcggtgtcg tcagccagaa actggttgat gatttcggcg acgatccccg cctggccttc
     3541 acgattgtga aagaagaaga actggacggc acgctcacca aaggattcca gcacacaggc
     3601 tattactcgc ggaaatacag ctccgatgcc gaacattggg gttccgacgg gcagtttgaa
     3661 ctgaaccgta cctgcaattt cagggtgatc cgcttctccg atgtgctgct gatggccgcc
     3721 gaattgggca gcccgaacgc gcagaactat ctcgacaggg tcagatcaag agttaacctg
     3781 ggaagcatcc cggccacgcc cgaaaatatc taccgtgaac gccgcctcga actttccctc
     3841 gaaggtatcc gctatttcga cgtgcttcgc aaagggattg cttttgcttc ccaggaattc
     3901 accgtagtgg atgtgcgcgg cccgaaatat accgacgaac aagtcgtata cgaccacacc
     3961 tttaactcag ccacgaaagg cttcctgccc atcccccaga ccgagatcga tatgtcggct
     4021 ggagtgctga agcagaatga aggatattga tcaagacagg ctgtcttaaa agtccataaa
     4081 ttcacttttt taggtagtcc gaccactaaa gccgtcggtt cctgagggat tttccttccg
     4141 acgactaaag ccgtcggttc catctttgta atattcttgg aaccaacggc tttagtcgtt
     4201 gaagcacgaa ttgttttcag gcgatcaaat cgaaatatca gacttttgtg acagtctcag
     4261 caatactaag gaatcagggg aacttattcg cataatttag cgttgcattc cccgcagaag
     4321 tacctctctt tcaaatcaat atcctccaca taggtgctcg aacgcatgat gcactgcggc
     4381 tggctgcagt gcaccagccc gtgcatatgc cccagttcat gcaaacaaac tttgatgatc
     4441 cggccggcca ggagcttatc atcctgcttc aggccgtata gctcattcct taaacgataa
     4501 gaggatacga tgccggactt gcctcccagg taagcctggc cgaaaatata ggtcagaatg
     4561 gggatgaaca agtcaacatt gaacaaggca aggatcttat acgaggattc caggggaagc
     4621 ttatccacaa cctccagtaa acgggtcccg ttatattgcc tcctccccgg atcataatac
     4681 aggctcaggt caaggtgccc ctcccggagt tccacctggc tttgaaattc ctgcgaaatg
     4741 ttctctgcaa tcctgtcaag taaggcttta tcaaaataac cgtatgatat caggctgata
     4801 ttctgcacgt tgctggttac cgctttactc ctggtttaaa tcatatttcc tgattttgtt
     4861 gtaaagtgtc accctgtcga tctgaagggc tttggctgcg cgcgaaatat tccagtcgta
     4921 acgtgtcaga atctgtacaa tgtgtttctt ctcctgctct tcgaggctct cggcagtgct
     4981 ggccgtggaa acttcccggc ccaggggcag atcttttacc cgtatctctt ttccgttacc
     5041 caccacaata gctctttcga tcatattctc cagctccctc acattccccg gaaaatcaaa
     5101 ctcctgcaaa cgcttcaggg ctgccggttc aatcgggatc acatcgcggc tcatcgaccg
     5161 gcaatacttc tttatgaaat attccgctaa aagaggtata tcttccggac gttcccgcaa
     5221 aggaggcaca tggatcccca cgacattcag cctgtaataa agatcttccc tgaaagttcc
     5281 gttcccgacc atcgccttca gatcgcggtt cgttgcacag attacccgga aatcactgga
     5341 aatctccctg ttgccgccta cccgcataaa acttctggtc tccaggaccc gtaaaagctc
     5401 aatctgcatt ttcatcgaaa cagtggcaat ctcgtcgaga aagatcgtac cgccgtcagc
     5461 catctcaaac cgccctttcc ggttgtacat cgcacccgta aaagcccctt tctcatgccc
     5521 gaataactcg ctttccagca aactctccgt caacgccccg caatgcacac tcaccagggg
     5581 aaaatagcgc ctggatgagt ttgcgtgtat ggccctggct atcaattctt tcccggtacc
     5641 gctttcgcca gtgatgatga cggaagcatt gctctgcgcc acactctcca cttcttttaa
     5701 tacctggagt attgccgggc tggtgccaat aatatcatcg atattctcaa gagatacaat
     5761 tttatctctc agggcatcat tttcggcctt tagcataatc tgcttcgaag cgttgcggat
     5821 caggtgcgac aggtcatccg ggtcaaaagg cttggtgata taatcgaatg cgccgtcttt
     5881 taatgcccgc actgccgaat cgacggtagc aaatgccgtc atgatgatga cgatcgcctc
     5941 cgggttcagg gttttgatcc gccgctgcat ctccagcccg tccatccccg gcatcttcaa
     6001 atctgccagc acaatgctga attccttctg ctcgatcagg ttcagcgcct ctttggcatt
     6061 ggccgcgcac tcaacatgat atccgtcttc gataaaccag ttgtacagcg aatccctcac
     6121 cgattcttca tcatccacaa tcaatattga aattttaccg gtcatgatct tttccttcaa
     6181 ggtttgctaa aggcaggata attgaaattt tggtgccgct tcccagccgg gaatcgacct
     6241 cgatccgccc tttatgattt aacacaatgc catgcgcaat ggaaagtccc agtccgatgc
     6301 cgctcgattt gttcttggct gagaaaaacg gttcgaaaat gtgcggaata tcatccgggg
     6361 caattccggt gccgttgtcg gtgatctcaa tccggatatg ctccccgtca ggattgaaag
     6421 tgcggacagc cacatcgccg ttttcgctga ccgcctccga agcattgacc aggatagcga
     6481 tacacgcttg cttgatctgg ttctccacgc atgataccac atcgttagac gccgcaaaat
     6541 cagtagtgaa ctgaatattc tcgaccttca tctggtgggc catcaggttg tgagtctcct
     6601 gcaaaacttc atttagatga cggttggaga aattttcctg gtccttcctt gaaaaatcca
     6661 gcaacccttt gacaatatcc ccgcatcgct tggtttccgt ttcaatcact ttcaaatgct
     6721 tctggatcga ctgcctggca ttctcatcca tgtccgcctt ccccaattgt ttggatacca
     6781 gttttgtata agtcagaact ccggcaagcg ggttgtttat ctcgtgtgca acagaagacg
     6841 acaatttccc cagcgaagca atcttctcaa tctggatcag ctcgttctgt gcttccagca
     6901 actcctccga ttttttctgg atcttatact ccagctggtg cgaccagttt tgtaattctt
     6961 tggtcgacgc ctggatttta tccagcatgt cattaaaagc tttcgataca actttgatat
     7021 catccagctc attcttgtca atttcaagcc tgactgaagt gtttcccttc ccgacagcct
     7081 cgctggcaag gatgatctca ttcagcggct tcttgatcct tttccgggtg aaaatgatca
     7141 gtatcgtggc catgatcagg gtcgaaagga tagaaaagaa aacaaaacgg gctgatgatt
     7201 tgctgactgt tgaatccagt tctgtcaacg gaaccttgat cagcaaggaa ccgagaaaag
     7261 tttcattcgc ctggtgggca tgacaatctg ccgtataaca ggtcttttcg ttcagaatag
     7321 gggaactgat aagcaattgc cggtaccctt taatggtttg gttcatcgta cataaactct
     7381 tcttgtcgat gatcttgtaa gatatctcgg ttttggaaaa catcccgctg aaatcttcat
     7441 gacacgttat gcaatcggga tttcccctgt cggcaatctc cgatgaaaaa accgaataga
     7501 tcaggtcgtt ccggttatta taaagattga cctcatcaat acccgatatc gagtggatcg
     7561 catccagggt ctgctgaaga gaggtttcat cattcgccag catcgattga tacagcgcac
     7621 cttttacaat cgaaccgaca ttgtgcccgt tctgtacaat aaccgaattc agatatttgt
     7681 gattgacaga acggaaaata atactaaacg aggcaaacaa tatgacagac attgcggcaa
     7741 ttatgatgac cacacgtgtg tatatagatg accctatttt gattctgacg ggtaaaacgg
     7801 acttaaaggc cccctttttt atcattacat catttttggg atgattttga tctcctaaat
     7861 taagaaattc ttgtatcgct taaatatttc agcgctttat catcctaata attaatgatg
     7921 atcgcttaaa aactcttcct gaaactcaac ccatatctgt tcaggcatct ctgccagtga
     7981 gccggccata atttcacctc cctcctgcag gatgatccct ttgccagtga gtgagtgacg
     8041 gaccatcgac tttgccagga aatccttgaa acggtccagc tctccgtacg tccagcttac
     8101 cgcctcttca cccagccggt aactattcgt ttcttctttc caggccgatg gactgatctc
     8161 atatatccac ccttgcccgt acggatcatc cgcaaacaaa tgtctctgtt cggcgagtga
     8221 atgattcggc cggcttatcg tacccgaaac cggagaaatg atctgcagtt ttttttcgcc
     8281 cacaccgact tctgcgatca aatcccctct tttgacttct tccccgggct tctttaaaaa
     8341 ttccaggtta acttctccgg ccagaagagc caggagatca tcaggaccaa tctttgccat
     8401 gcccgatttt tccatgaatg cccaggtatg catcggactg atgaaaaccc cgcggggcac
     8461 ccgcaatgta tccctggtca aaaacctgac ggcctttttg atctttgttt tcaggtagtc
     8521 atttttattg atgatgatcc agaaagggat cagcaacagc aggaacagaa tgatgatgat
     8581 atattctata cccttggtct gaaaaatatc atgataagta aaaccgtcca tcttatattt
     8641 ttttaagatt aattaatgaa ttgtttctca atgattttta gcccattccg gggaatccct
     8701 gagaaccggc atccggttga tgatccaccg gaatatccat atttccgtga aaatcaccgc
     8761 cagtgtcacc acgatctcca tccaggacgg gaaataatga ggttctaaat tccatttgaa
     8821 accgataaca gtcacattca aacgattaat gataatcccc agaatggtga taatagcggc
     8881 aatctttacc agtaaggcat tgtgccggcg atagctggtg aaaaacaaca gcatcgggaa
     8941 cagaacgaaa ccgatcattt ctaccaggta ccagtatccc atgggggttc caaggtattc
     9001 ccaccgctgc ccgtgcacaa atatcaggat ctgcaggaag aaataagcaa acattgccac
     9061 tgcgcatatc ttcgacaacc cgatcacaat gccgtgatgg gctttacggt tcggctcact
     9121 gatctggtga aagaatacct tatgactgat ggatccctca aaaatgacca gcgacaatcc
     9181 cgcaaagatg ctgctgacaa aaaacatgat cgggatgaat tcgttgtacc aaagcgggtg
     9241 gatcttttct ttcgccatga ggtaaagcgc accgagaccc gactggtgca acgtcgacag
     9301 ggtaataccg aaaatgacgg ccgcaatggt catccccgaa aggatcctat gcgcccgcct
     9361 ggcccccagc cattcggcta tggcaggcga aaactcgatc agttgtgcaa gcatgtaaag
     9421 caggaaatgc catgccacca ggaacaatac cgaactgtac ccgaagtcgt taccgatgat
     9481 cggattgatc acgttccagg gcctgccgag atccagcagc aatgcgccgg cataaaatac
     9541 gtaagccagg aaaccgttca aaacggttac ccttacgatt gaatggtatt tcttcatgtt
     9601 caggatgtag accatgaagg tcagtacgta ggcgccacct gcaaaagcca cgcccgtgac
     9661 cacatcaaac ccgatccaga gcccccacgg gacctcctgc gtcaggttcg ttaccgatcc
     9721 gatccctttc cagaaccgga tgacgataag caccaatccg gtcagcatga tcggaaatga
     9781 aataatattg aatggggtga aaatcttacc cctgggtttc atctctttca ggataaattt
     9841 ccagaaaccc tctttttgcc ggtcggcttc caaagtatat tcgcctttaa tcatggctct
     9901 cttcatttaa aggtttatct tttgtagctt ctcttattcc aagcaaaatg gccggccaga
     9961 gaacgaaaat ggtggggacg ctgtagagga accctttgga taattcggga taggaggatt
    10021 tctggatgga ggtattgaac cccagttctt caaaagggac gggagaaagg tagaggaatc
    10081 ctgtgccgcc tgcttcatgc tctccgtata tgtgatcgta gtaaacatcg ggattgtcgt
    10141 ggatcctcct cctggcttct ttgatcatct ctctccgggt tccgaacatc agggcttcgg
    10201 ccgggcattc ggtaacacag gcgggcataa ggccttcctt aagccggtca aagcacatgt
    10261 cgcatttcct gatttccgga ttggggctgt gatattcgaa tttaggaata tcaaacgggc
    10321 aggaaaccat gcaataacgg caccccatgc atttattgcc ccgccagatc acaggcccgg
    10381 tatgggtttt atgcatggca ttcgtcaggc aggccgaagc acaggcaggc gagttgcaat
    10441 gcatgcattg ctgcttgacg taaacttctc ctttcgacgt gttgtacaaa ttgatcacat
    10501 tctgctgtgt ttcgctcgcc ttgcggatga tccccgcttc cggcgaatcg gcgggctcag
    10561 gaaggttgtg cgccgccgca caggcatact cgcagctctg gcatccgaca cagcggctgg
    10621 catcgtaaag cacccccatg aattcttccg gcggcgcatc ttttgctgcc tctgttgtgg
    10681 cggcattcaa ttttttcccg gtggccagcg ctacgcctgt aacccccagt accttgaaaa
    10741 aatttcgtct gtcaattggc ataatgggtg taatataatg atgaaactat attatttacc
    10801 tgtgattacc ttgctttgtc aaggaaagat gtctggaaat cttcccacac ctccggcccg
    10861 aaatcggcca ggacatgatc tttcaaatgt cctccatcct gcaaaacaac atactgcaat
    10921 tcgggacggc ttactttgat tgaggtcgcc aggaaatccc tcagccttga aaattcatcc
    10981 gaaatccact tcctgtagct gtcggccatc tccatgaact gaagctcttt catccagttt
    11041 gccggctcaa cccggtaaag ccagcccgat gtatagggcg aggcattcaa ttgcgatgga
    11101 tcggatttta aaagtggatt gaactcggct atggtccctg agaccggtga ataaagatgg
    11161 agctgcttgc cattttgaac cagcttaacc aggatatcgc ctttccggat cttctctccg
    11221 gctttcttca tttctacccg tgtcacaggt cctacgatgt gttgcatgaa atcatcgatc
    11281 ccgattttta ccttcccgtc acggtccata aaactccagg tgtgcgattt tgaaaaaagc
    11341 agtccctgcg ggatacctat ggattcgggg ttcagttgcc cccgggcgat tttttccgga
    11401 gaaggctgaa cacctccctt tttggcaaaa aataacaggg ccgaaaccag cagtgcaaca
    11461 aaaagaaacg ataaaatgac gatcaaagcg acattggaat ggaagatgtt tcctgaagga
    11521 tcgtccggtg attggcggtt aatgaggcgt tcggccttgg ccagcctctc gccggcgacc
    11581 aaccccgaaa aaccggc
//
LOCUS       DAOQXS010000020        11385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6140_length_11385_cov_3.692674, whole genome shotgun sequence.
ACCESSION   DAOQXS010000020 DAOQXS010000000
VERSION     DAOQXS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6140_length_11385_cov_3.692674"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..730
                     /locus_tag="PKM34_01230"
     CDS             143..730
                     /locus_tag="PKM34_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ82231.1"
                     /translation="MKAAIISDQELIGKYLNGNEESLELLINRHKRKVFAYIIMVVKD
                     DQLADDIFQDTFIKVINTIRSGSYKEEGKFIQWVMRIAHNLIIDHYRRAKRLPVVNNS
                     SDDFDIFNLVRITDPSVEEQIITSQIHEDVRNLIELLPNEQKEVLMMRHYSDMSFKEI
                     AEATDVSINTALGRMRYALINLRKIVKEKNIILTV"
     gene            814..1008
                     /locus_tag="PKM34_01235"
     CDS             814..1008
                     /locus_tag="PKM34_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82232.1"
                     /translation="MDKTFTPLDIQRYLLEIRRIEFDSDKKKADSGPAALTIRNILNY
                     SRSLNIYKTSIMGNIFHVSN"
     gene            1241..1897
                     /gene="nth"
                     /locus_tag="PKM34_01240"
     CDS             1241..1897
                     /gene="nth"
                     /locus_tag="PKM34_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNQ82233.1"
                     /translation="MKKTERFKAFMAYFEENMPVAETELLYSNPYELLVSVILSAQCT
                     DKRVNMITPSFFRRFPDVGSLASAEKEEVFELVKSCSYPNNKSLHLLNMARMVMEKFG
                     GVIPDNTEDLQKLPGVGRKTAHVMVSVIYNQPKMAVDTHVFRVAARIGLTTGAKNPLQ
                     TELQLVKHIPEEKIPLAHHWLILHGRYVCRARNPACDECGMKPYCRFYEKLSKRQNLT
                     "
     gene            1988..2446
                     /gene="bcp"
                     /locus_tag="PKM34_01245"
     CDS             1988..2446
                     /gene="bcp"
                     /locus_tag="PKM34_01245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNQ82234.1"
                     /translation="MNVKIGDKAPSISALDQNSNAFTLDSLRGSKVILYFYPKDDTPG
                     CTAEACNLRDNYSDLLNRGFKIVGVSTDSVKSHQKFSEKYSLPFPLLADTDKKIVNDY
                     GVWGKKKFMGREYDGTHRVTFVLSEEGTVLKIFDKVQTKEHAQQILMEIN"
     gene            2538..3554
                     /gene="recA"
                     /locus_tag="PKM34_01250"
     CDS             2538..3554
                     /gene="recA"
                     /locus_tag="PKM34_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNQ82235.1"
                     /translation="MGGENNVDQTRKEKMKALEMTLGNIEKQYGKGTIMKLGDTPAEN
                     IPFIPTGSIGLDAALGVGGFPRGRVVEIYGPESSGKTTLALHAIAEAQKLGGIAAFID
                     AEHAFDRFYAQKLGIDIDNLLISQPDHGEQALEIVDNLIRSGAIDIIVIDSVAALTPK
                     SEIEGEMGDSKMGLQARLMSQALRKLTANIGRTGSCCIFINQLREKIGIMFGNPETTT
                     GGNALKFYASVRIDIRKINQIKEGEENTGNRVKVKVVKNKVAPPFKKAEFDIIYGQGI
                     SRIGEIIDMGSDFGIIKKSGSWFSYGETKLGQGRDAVKKLLTDNPELGEELEAKIREK
                     LIQS"
     gene            3578..4597
                     /locus_tag="PKM34_01255"
     CDS             3578..4597
                     /locus_tag="PKM34_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82236.1"
                     /translation="MNRIAVFLFCLACLWAVSCTSPPARDKVPGESHKADTTVTVIER
                     LNRQIASDSANPDHYYRRSLYYLDKKEINQALSDIGKAIGFNDKNSSYFVTLSDVYLA
                     MGRIPSCLEALKKAESLDPANRDALLKMAEVYLYLRDYKNVQDYTKKALEIDRNSPTA
                     YFIRGYAALEQGDTSLAVQNFQRTVDLDQNHYGASIQLGGLFAEKNPALAAGYFQSAI
                     RIDPNRPEGYYLLGFLYQNQENIDKAIAAYEKLIVIAPEYKEAYYNLGYVNLVYTNDF
                     EASVRYFSQAVLLDSKYTDAFFNRGYSYELMGDVVNARKDYLRALEITPNYENAINGL
                     NRLDK"
     gene            complement(4577..7024)
                     /gene="feoB"
                     /locus_tag="PKM34_01260"
     CDS             complement(4577..7024)
                     /gene="feoB"
                     /locus_tag="PKM34_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNQ82237.1"
                     /translation="MRSLRELKEGEKGIIIRIRGKGEFRKRIIEMGFVKGKRVTVIKA
                     APLQDPVEYRILDSDVSLRLEEADLIDVIPEAESPGFLEPGNFSSIFNEPDPLEEFHR
                     NGKIIHVALVGNPNSGKTSLFNHASHSREHVGNYAGVTVDSKTAEVKTGDHIIRLTDL
                     PGTYSLSSYSPEELFVRNHLSEKRPDLVINVIDASNLERNLYLTTQLVEMGLNVIVAL
                     NMYDELQIKGDRFDYEMLGKMTGIHFVPTIASKGKGIKELFAKIIDVYTGKTVTKRRI
                     RLDYGPEVELSVSRLMSRFHGTIVGESFPDRYFAVKLLERDWHALHYLEHKCKNPDIC
                     KLAEAETQRLEKIFNEDTESLIAGIRYGFIEGALKETYIPSTTPRMTLTRRIDFFLTG
                     RFAGYPIFILFMWLMFQATFIFGDYPVYLIEKLVEFTGNLLDRIIPDGIINDLVVDGI
                     IGGVGGVIVFLPNILILFLFISLMEDTGYMARVAFIVDKLMHKIGLHGKSFVPLLMGF
                     GCNVPAIMATRTIESRSDRMVTMLIIPFMSCSARYPVYILLISAFFVSYKGTILFILY
                     LTGIIMAAAVAFLLKKTLFKAKEIPFVMELPPYRMPTAKAILKHTWFKGVQYLRKMGS
                     VILVASVIIWALGYFPRNHERKAHYEAMISQATDEAKIHDLRSAMAREQQEQSYIGRL
                     GKFIEPVIRPLGFDWRMGVSLVAGSAAKEVVVSTMGVLYQTGSDEGVTGLIGKLQQQK
                     YTQGPRQGEPVFTPLVAFAFMIFILIYFPCIAVIAAIRKESGRWKWPAFVAVYTTALA
                     WLAAFAVYQIGLLIQPV"
     gene            complement(7148..7699)
                     /locus_tag="PKM34_01265"
     CDS             complement(7148..7699)
                     /locus_tag="PKM34_01265"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNQ82238.1"
                     /translation="MEKINPPYTEPENSLSVLELLRVAHEYCIYCEKASEKPFSENVD
                     FILKISPLLYLKGKLLPEINPEYPEFAERYVTEEEWQEVFNHFRSVYLEKDEFYNVSY
                     EDHHESEAVKSSLSENLSDIYQDLKDFILLYQKNTQAARQNAVSQCRDLFETRWGPKL
                     LSNLRYLHFLSVKERLPGMDDER"
     gene            7815..8267
                     /locus_tag="PKM34_01270"
     CDS             7815..8267
                     /locus_tag="PKM34_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82239.1"
                     /translation="MDRLKLKQKILDACFEQQTKVVDNLKVVMEEVADSAEEHGLPKD
                     LYDSYRNQMMSKKEMFAQQLLKVNEQIDILRRVDMTRVFQQVRFGAVVVTESQRLFIA
                     SGIGKIIVEGLEYFVISAMVPFYSAIDGKKPGDSYEFRGKKEKILEVF"
     gene            complement(8315..8524)
                     /locus_tag="PKM34_01275"
     CDS             complement(8315..8524)
                     /locus_tag="PKM34_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ82240.1"
                     /translation="MNNRIREFRARYNLTQDDLALKVGVRRETIVFLEKGKYNPSLKL
                     AHDISVVFNVKIEEIFLFDDPAPST"
     gene            complement(8517..8915)
                     /locus_tag="PKM34_01280"
     CDS             complement(8517..8915)
                     /locus_tag="PKM34_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82241.1"
                     /translation="MKRSIVIFIIAAMVLITTGFWILSPGAIGDTMEYLHFGIILLIV
                     LFALFIGYKRLKSEKRGEPAEDELSKKVLQKTAAWSYYISLYIWVAMIYIKDRIVMDT
                     EIMLGTGILAMAVTFAVCWLVFNFRGIRNE"
     gene            9217..>11385
                     /locus_tag="PKM34_01285"
     CDS             9217..>11385
                     /locus_tag="PKM34_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNQ82242.1"
                     /translation="MKHLLIIVFFLGTTASLLAQNMQVSGVITDESTGETLIGATVLE
                     KGTTNGTVSDLDGKYTISVPKGATLAFSLVGYEPEEFVIEKSGTLNVAMKVQVTNLDD
                     VIVIGYSTQKKSDKTGAVSLVKADEMNSGVITSPIQSMQGKAAGVLVTKKGGDPNESF
                     SVRIRGASGFNSNTQPLYVIDGIPGADPTILSPSDIESFNILKDAASTAIYGSRGSNG
                     VIIITTKKGKKSGIPAEGDKTEGAFSNIEFTSQVSFEQISKKLGILDADGMRSFANKL
                     LEEAQVENPNYTIDSVFTDGGASTDWQDEIYRWGVSTVNNLSFSGGNKHNLYLASITN
                     SGWQGIMEGTEKKLTTARVNVTHKGFNDRLTITGNLMGAFEKNDYENYDGWDKDDIIY
                     QAISRNPTDPVYDSTGEYYKTSRVFNYENPLAIINYVTNKRDANKFLGGVKADFEIVK
                     GLVAGVNLGYINDNQTSTYFRPSGIYSSADNGYGKKEYKNNTQKLIEITGTYNKTFNK
                     NHNFNALLGYSWQESVYSGFYSQGGDAQSNYAGPDNLAVLNDVKWGDIGSWKGKWNLI
                     GFFGRVQYNYKSKYYASASLRRDGSSKFGSNNKWGWFPTAAIGWNLDQEKWLSDVDWL
                     SQLKIRASYGVSGNQEIGEYRSMVLWEPSGKAINPETGQEVITFKPAWNANPDLKWEE
                     TSEANLGIDFAFLNSRLSGSLELYYKITNDLLGEYNVPVPP"
BASE COUNT         3216 a         2539 c         2658 g         2972 t
ORIGIN      
        1 tttatttttt tattgactat atttgcactt ctttcatcga aagtaaattg aaatatttca
       61 tctgaccttt aaattacagg aggaaaatta tcgattaaat atttaccctt ttttacagta
      121 ttcacctaaa aaaggaggca ctatgaaagc tgccatcatt agcgatcagg agctaattgg
      181 taaatattta aacggtaatg aggagagtct ggaattactc atcaaccgtc acaaacgcaa
      241 agtttttgca tatatcatca tggtcgttaa agatgaccag cttgctgatg atattttcca
      301 ggatactttc ataaaggtga tcaacacgat tcgttcaggc tcatacaagg aagagggcaa
      361 gttcatccaa tgggtcatgc ggattgccca taacctgatc atcgaccatt accggcgggc
      421 aaagcgattg ccggtggtta acaattccag cgacgatttt gatattttca acctggtcag
      481 gataaccgat ccttccgtgg aagagcagat cattaccagc cagatccatg aagatgtcag
      541 aaatctcatt gaactgcttc cgaatgagca aaaggaagtg ctgatgatgc gccattacag
      601 cgacatgagt tttaaagaga tcgccgaagc gaccgatgtc agtatcaata cagccctcgg
      661 ccggatgcgt tatgcgctga tcaacctcag gaaaattgtt aaagagaaga atattatcct
      721 gacagtttaa cttatagttt ttttcgtatt ccaaaaataa ataccggttt tatccgtttg
      781 cctgttatag ttccttaaca actaaatcag cctatggata aaacctttac cccacttgac
      841 atacaacgct atttactgga gatcaggcga atagaatttg actcggataa aaagaaagcc
      901 gattcaggcc cggcagcgtt gactatcagg aatatcctga actattcccg gtcgttaaat
      961 atctataaga cctcaataat gggcaacatc tttcatgttt cgaattgaga gaggagtagt
     1021 ccttaaaaca tattctgttt gttcagatta tagtccgacg ggtgaatccg tcggtacata
     1081 atggtttttt cttctgaata tctgtcaaaa tcagatatta aatcattttt cataactcat
     1141 tgcttttcag attgatatac caaatatttt catgttgaaa aatttatttc ataaccaccg
     1201 ggtatttttt acctgttttt ccgattaatc attatagaag atgaaaaaaa cggaacggtt
     1261 taaagctttt atggcctatt ttgaagaaaa catgccggtt gccgaaaccg agctgctgta
     1321 ttcaaatcca tatgaattgc tggtatctgt cattttatcg gcacaatgta ccgacaaaag
     1381 ggtcaatatg attaccccgt cgtttttcag gcggtttccc gatgtggggt cactggcatc
     1441 ggcagaaaag gaggaggtgt tcgaacttgt aaaaagttgt tcctatccca acaataaatc
     1501 gttgcatctg ttaaatatgg caagaatggt tatggaaaag ttcggggggg ttatccctga
     1561 taacacggaa gatcttcaga aacttcccgg agtggggcgc aaaacagctc atgttatggt
     1621 ttcggttatt tataaccagc cgaagatggc agttgatacg cacgttttca gggttgctgc
     1681 acggattggc ctgacgaccg gtgctaaaaa cccgctgcag acggaattgc agctggtaaa
     1741 gcacattccg gaggaaaaaa taccgctggc ccaccattgg ctgattctgc acggaagata
     1801 tgtatgccgt gccaggaatc ctgcatgtga tgaatgtggg atgaaacctt actgccggtt
     1861 ttatgaaaaa ttgtcgaagc ggcagaattt gacgtgaagt tgttcgtaaa ccttgataac
     1921 taatgccttt aggttaatca taaaacaatt aaaatcaata tttttgtcat tattaaaatt
     1981 aacttttatg aatgtaaaaa ttggggataa agccccgtcg atcagcgctc tggaccagaa
     2041 tagcaatgcc tttaccctgg atagtctccg gggcagcaaa gtgatcctct atttttatcc
     2101 gaaagatgat acgccgggat gtacagccga agcctgtaat ttgcgtgata attattcgga
     2161 cttgctgaac cgtggtttca aaattgttgg cgtgagcacc gacagtgtga aatcgcacca
     2221 gaagttttct gaaaaatatt ccctgccatt ccctttactg gccgataccg ataaaaaaat
     2281 agtcaacgat tatggtgtat ggggaaagaa gaagtttatg ggaagggaat atgacggaac
     2341 gcaccgggtt accttcgttc tttcggaaga agggactgtt ttgaagatat ttgataaagt
     2401 ccagacaaaa gaacatgctc agcagatcct gatggaaatc aattaaaata gtaacatcca
     2461 aacagacaaa tcaaaatcaa aatcaaaatc aaaaatcttc aaatccacaa atctacaaat
     2521 ccacaaatct taatattatg ggcggagaga acaacgtcga ccagacaagg aaagaaaaga
     2581 tgaaagccct ggaaatgacc ctgggcaaca ttgagaaaca gtatggaaaa gggactatca
     2641 tgaaattggg tgatacacct gctgagaata ttccttttat tccaacgggc agtatcgggc
     2701 tcgatgcggc attgggagtc ggcggattcc ccaggggaag ggtggttgaa atttacggcc
     2761 cggaatccag cggaaaaacc acactggcgc tgcatgctat cgccgaagca caaaaactgg
     2821 gggggattgc agcattcatc gatgccgagc atgctttcga ccgtttctac gcgcagaagc
     2881 tgggcatcga tatcgacaac ctgctgatct cccagccgga tcacggggaa caggccctgg
     2941 agatagtgga taacctgatc cggtcgggag cgatcgatat tattgttatt gactccgttg
     3001 cggcgctaac ccccaaaagt gagatcgaag gcgagatggg cgactccaag atgggattgc
     3061 aggccaggct gatgtcgcag gccctccgga aactgactgc caatatcggc aggaccgggt
     3121 cttgctgtat ttttatcaac cagctcaggg aaaagatcgg tatcatgttc ggtaaccccg
     3181 aaacaacaac cggtggaaat gccctgaaat tctatgcttc ggtcaggatt gacatccgca
     3241 agatcaacca gatcaaggag ggggaagaga ataccgggaa cagggtcaag gtgaaagtag
     3301 tcaagaataa agtggcccct ccgttcaaaa aggctgaatt cgatataata tacggccagg
     3361 ggatcagccg tatcggcgag atcatcgata tgggctccga tttcgggatc attaaaaaga
     3421 gcggttcctg gttctcatac ggagaaacaa aactcggaca gggccgggat gcggtaaaaa
     3481 agttgcttac ggataatccc gaactgggtg aagaactgga ggcaaaaatc cgcgaaaagc
     3541 tcattcagtc ctgaacctgc ttatttatcc ctttgaaatg aaccggattg ctgtattcct
     3601 tttctgtctt gcctgcctgt gggcggtttc ctgcaccagt cccccggccc gcgacaaggt
     3661 gccgggtgaa tcccataaag ccgatacaac ggtaaccgtt attgaacgtc ttaaccggca
     3721 aattgcatcc gattcggcca acccggatca ttattaccgc cgctcgcttt attatctcga
     3781 caaaaaagag atcaaccagg ccttatcgga tatcgggaaa gccattgggt tcaatgataa
     3841 gaattcctcc tatttcgtga ctttatcgga tgtttacctg gcgatgggcc gcatacccag
     3901 ctgcctggag gccctgaaga aggctgaatc actcgatccg gcaaacagag atgcattgct
     3961 gaagatggct gaagtttacc tttatctgag ggattacaaa aatgtccagg actacacaaa
     4021 aaaggcactg gaaatcgacc ggaacagtcc tacagcttat tttatccggg gatatgcggc
     4081 gctggaacag ggagatacgt cactcgctgt ccagaatttc cagcgtacgg tcgacctcga
     4141 ccagaaccat tatggcgcca gtatccagtt aggcggttta tttgccgaaa agaatccggc
     4201 attggcggcg ggatatttcc agtcggccat ccggatcgat cctaaccggc cggaaggata
     4261 ttacctgctg ggatttcttt accagaacca ggaaaatatc gataaagcca ttgctgccta
     4321 tgaaaagctc atcgtgatcg ccccggagta taaagaagct tattataacc ttggttatgt
     4381 gaatctggtg tatacgaatg attttgaggc atccgtgagg tatttctcgc aagccgtgct
     4441 ccttgattct aaatataccg atgccttttt caacaggggt tacagttatg aactgatggg
     4501 cgatgttgtc aacgcccgga aagactatct ccgagcgctg gagataacgc cgaattatga
     4561 gaatgccatc aacggattaa accggttgga taagtagccc gatctggtaa acggcgaatg
     4621 ccgccagcca ggccagcgcc gtggtataaa cggcaacaaa cgccggccat ttccatcttc
     4681 ccgattcttt tcttatggcc gcaatgaccg cgatgcaagg aaaatagatc aggatgaaga
     4741 tcataaaagc aaatgcgacc agaggggtga atacgggctc tccctgccgg ggcccttgcg
     4801 tgtatttttg ctgttgcagc ttaccgataa gccctgtaac tccttcgtcg ctaccggttt
     4861 ggtagagaac acccatcgtg ctgacgacca cctctttggc tgccgaaccg gctacaaggc
     4921 tgacacccat tctccagtcg aatcctaacg gacggatcac cggttcgatg aatttgccca
     4981 gtcttccgat atacgattgt tcctgctgct cgcgggccat cgccgaccga aggtcatgga
     5041 tcttagcctc atcggttgcc tggctgatca tagcctcata atgagctttt cgttcatggt
     5101 tgcggggaaa gtatcccagc gcccagataa tgaccgaagc cacaagaatg acactcccca
     5161 tctttctcag gtactgcacc cctttgaacc aggtgtgttt gaggatggct ttggccgttg
     5221 gcatcctgta gggaggcagt tccatcacga aagggatctc ttttgccttg aataaggttt
     5281 ttttcagcag gaaggccacc gccgccgcca tgattattcc cgtgaggtac agaataaaca
     5341 ggatcgtacc tttatagctg acaaagaacg cactgatcag caggatataa accgggtagc
     5401 gggccgagca cgacatgaaa gggatgatga gcatcgtaac catccggtcg cttcggctct
     5461 caatggtgcg ggtcgccata atcgccggga cattgcagcc gaagcccatc agcagtggga
     5521 cgaacgattt gccatgaagc ccgattttat gcatgagctt gtcaacgata aaagccacac
     5581 gggccatata ccccgtatct tccatcaggg aaatgaaaag aaacaggatg agaatattcg
     5641 gcaggaaaac gatcacgcca cctacacccc cgataatgcc atctacgacc agatcattga
     5701 tgatgccatc gggtatgatc ctgtctaata aattaccggt gaattcaacc agtttttcaa
     5761 tcaaataaac cggataatca ccgaaaataa aagtggcctg gaacatcagc cacatgaaga
     5821 ggataaaaat cgggtatccc gcaaaccggc cggtcagaaa gaaatcgatg cgccgggtca
     5881 gggtcatcct gggcgttgtc gacgggatat aagtctcttt cagagctccc tcgataaatc
     5941 cataccggat accggcaatc aggctttcgg tgtcttcatt gaatatcttt tccagccgct
     6001 gggtctcagc ctcagctagt ttgcatatat cgggattttt gcatttatgc tctaaataat
     6061 gcaaagcgtg ccagtccctt tccagtaatt tgaccgcaaa atacctgtcg gggaaggatt
     6121 cgcccacaat agtaccatgg aaacggctca tcaggcggct gaccgacaat tccacctccg
     6181 gcccgtaatc caggcggatt cttctttttg taacggtttt accggtataa acatcgatta
     6241 ttttggcaaa taattctttg attccctttc ctttagacgc gatggtaggg acgaaatgaa
     6301 tacctgtcat cttgcccagc atctcgtaat caaaccggtc gcctttgatc tgaagctcat
     6361 catacatatt cagggcaaca atcacattga gccccatttc taccaactgt gtggtcaggt
     6421 aaagatttct ctccaggttg gaagcatcga tcacgttgat gaccaggtcg ggacgtttct
     6481 ccgacagatg gttcctgacg aaaagctctt ccgggctgta ggatgagagc gagtaggttc
     6541 ccggcaggtc ggtaaggcgg atgatatggt cgccggtttt cacctccgct gttttcgaat
     6601 ccaccgttac cccggcataa tttcccacgt gttcgcggga gtgagaagca tggttgaaca
     6661 ggctggtttt gcccgaatta gggtttccaa ccagggctac atgaatgatc tttccgtttc
     6721 tatggaattc ctcgagcgga tcaggctcat taaagatgct gctgaaattc cccggttcca
     6781 ggaatccggg cgattccgct tccgggatga catcgatcag atccgcttct tcaagcctca
     6841 gagatacatc tgaatcaagt atccggtatt ccacaggatc ctgtaacggg gcggctttaa
     6901 taacggtcac ccgttttcct ttcacaaagc ccatttcgat gatcctttta cggaactcac
     6961 cttttcccct gatccggata atgatccctt tttctccttc ttttaattcc ctgaggcttc
     7021 tcatttattt agattaattc cagataacgg ggacaaaaat atcgaaataa gaagtaggtc
     7081 agcagggtag cggttctaaa ttatttctaa ataagtggca gagttttttg cgactaacga
     7141 cttacgacta acgctcatca tccatacccg gcaacctttc ctttacggag aggaaatgca
     7201 ggtagcgcag attgctcagc aacttcggcc cccagcgtgt ttcaaacaga tccctgcact
     7261 gactgacggc attttggcgg gcggcctgtg tatttttctg gtacaggagg atgaaatctt
     7321 tgaggtcctg gtatatatcc gaaagatttt cggaaaggct tgatttaacg gcttccgatt
     7381 catggtgatc ttcgtaactg acattgtaga actcatcctt ctcaaggtac accgatctga
     7441 aatggttaaa cacttcctgc cattcctctt ctgttacata ccgttcggcg aattccgggt
     7501 attccgggtt gatttccgga agcagtttgc cttttagata cagcaacggg ctgattttca
     7561 atataaaatc cacattctca gaaaatggtt tttctgaggc tttttcacaa taaatgcaat
     7621 attcatgggc aacgcggagc aattcaagta cgctaagaga attttccggt tcggtgtaag
     7681 gcggattaat tttttccata aatgaaaata ttattatttt cgtacaaaat ttgttatttg
     7741 cttttacctt tcaaaatctt ctcgtgagtt ttcgcgataa gatttttaat ttttaaaaat
     7801 attataaata tagtatggat cgtttgaaac tgaagcaaaa gatcctggat gcctgttttg
     7861 aacagcagac caaggtggtg gataacctga aagtggtgat ggaggaagtg gccgacagcg
     7921 cagaggagca tggcctgccg aaagatttgt atgattcgta ccgcaaccag atgatgagta
     7981 aaaaagagat gtttgcccag caattgttga aggtaaatga gcaaatagat atattgcggc
     8041 gggtggatat gacccgtgtt tttcagcagg tcaggtttgg cgcggtcgtc gtaaccgaat
     8101 cccagcgact atttatagcg tcaggcatcg gcaaaattat cgtcgaaggc ctggaatatt
     8161 tcgttatttc cgccatggtc ccattttaca gtgcaatcga cggcaaaaaa ccaggcgatt
     8221 cctacgaatt cagaggaaag aaggagaaga tcctggaggt gttttagtgc aggtcgatat
     8281 gtgtagtcat aagtcgttgg tggtcagtcg ttagtcaggt tgagggtgcg gggtcatcaa
     8341 aaagaaagat ctcttcaatc tttacattaa acaccaccga gatatcgtga gccagtttca
     8401 gcgagggatt gtacttgcct ttctctaaaa aaacaatggt ttcccttctt acgccgactt
     8461 ttaacgccag gtcgtcctgt gtcaggttgt acctggccct gaattccctg atccggttat
     8521 tcattcctga tcccccggaa gttgaaaacc agccagcaaa cggcaaaagt gaccgccatt
     8581 gccaggatcc ctgttcccag cataatttcg gtatccatga caatacggtc tttgatgtag
     8641 atcatggcaa cccagatata gagcgaaatg taataggacc aggcagcagt tttctgcagt
     8701 actttttttg acagttcatc ttcagccggc tcgcctcttt tttcgctctt caggcgttta
     8761 tagcctataa ataaggcgaa cagaacaatc agcaggatga taccaaaatg cagatactcc
     8821 attgtatctc caatcgcccc cgggctgaga atccaaaaac cggttgtgat cagcaccatt
     8881 gcggcaatga taaatattac aattgatctt ttcatatgaa taaatttgat gttataaata
     8941 cctaacacaa agttagaaat aaataacatt ctaatccaaa attattttaa tcttttttta
     9001 acatttattt tattattttt gctgtgcttt ataaccaagg atgcatggaa tgtgccgttg
     9061 attcagcatt ttgcaaagtt ttacatggtt cattattttg ggaggttcaa aactcaggcc
     9121 ccgaaatcac tattttgcag tgttcgacta tcttattctt aacccgattt ccagaaacta
     9181 atcttcataa atatgaaaaa taaacttaga ataattatga aacacctgtt aatcatcgtt
     9241 ttttttctgg ggacgacggc cagcctgttg gcccagaaca tgcaggtttc cggtgtgatc
     9301 accgacgaaa gcacgggtga gacgcttatt ggcgctacag tgcttgaaaa gggaaccaca
     9361 aacggtactg tcagcgacct ggatggcaaa tacaccattt cggtacctaa aggggccaca
     9421 ctggccttct cccttgtcgg gtatgagcct gaggagttcg ttatcgaaaa atcggggacc
     9481 ctgaatgtcg ccatgaaagt ccaggtgaca aatctcgacg acgtcatcgt catcggttac
     9541 agcacccaga agaaatcgga taagaccggt gctgtcagcc tggtgaaagc ggatgagatg
     9601 aacagcgggg taatcacctc accgatccag tcgatgcagg gtaaagcggc cggtgtgctg
     9661 gtaacaaaaa aagggggcga cccgaatgag tcattctccg tcaggatacg tggcgcttcc
     9721 ggttttaatt ccaataccca gcctttatat gtcattgacg ggattcccgg cgccgatccg
     9781 accattcttt cacccagtga tatcgaatct ttcaatattt tgaaggatgc agcttcgacc
     9841 gctatctacg ggtcaagggg atcgaacggt gtcatcatca ttaccacaaa gaaagggaag
     9901 aaaagcggta tcccggctga aggtgacaag acagaagggg ctttcagcaa tatagaattc
     9961 acctcccagg tatctttcga gcagatctcc aagaagctgg gcatacttga tgcggacggg
    10021 atgagatctt ttgccaacaa actcctggaa gaggcacagg ttgagaatcc aaattacact
    10081 attgacagcg tttttacaga tggaggcgcc tcgaccgact ggcaggatga aatatacagg
    10141 tggggcgtat ctacggtcaa caaccttagt ttttccggtg gaaacaaaca taacctctac
    10201 cttgcctcca tcaccaattc gggatggcag gggattatgg aaggaaccga gaaaaaactg
    10261 accactgccc gtgtcaacgt gacgcataaa gggttcaatg accggctcac cattaccggt
    10321 aacctgatgg gtgcgtttga aaagaacgat tacgaaaatt atgacggatg ggataaagat
    10381 gatattatct accaggctat ttcccggaat cctaccgatc cggtatacga ttcgaccgga
    10441 gagtattata aaacttcccg tgttttcaat tatgaaaatc cgctggcgat catcaattac
    10501 gtcaccaaca agcgcgacgc caacaaattc ctgggtgggg taaaagccga cttcgaaatt
    10561 gtgaagggat tggttgcagg agtcaatcta ggttatatta atgataatca gacttcaaca
    10621 tatttccgtc cttccggaat atattcctct gccgacaatg gttatggcaa aaaggagtac
    10681 aaaaacaaca cgcagaaact gatagaaata accggcacct acaataaaac ctttaacaaa
    10741 aaccacaatt tcaatgcttt actgggatat tcctggcagg aatcggttta cagcggtttt
    10801 tattcacagg gtggcgacgc ccagtcgaac tatgccgggc cggataatct ggccgtactc
    10861 aacgatgtga aatggggcga cattggctca tggaaaggaa agtggaacct gatcggtttc
    10921 ttcggcaggg ttcaatacaa ttataaaagc aaatattatg cttcggcttc actacgccgc
    10981 gacggttcat ccaaattcgg ttctaacaat aaatggggat ggttcccgac cgcagcgatc
    11041 ggctggaacc tcgaccagga aaaatggcta tcggatgttg attggctgag ccagttgaag
    11101 atcagggcca gttacggtgt ttccggcaac caggagatcg gtgagtaccg cagtatggtt
    11161 ttatgggaac ccagcggaaa agccatcaat ccggagaccg ggcaggaagt catcactttt
    11221 aagcctgcat ggaatgccaa tcccgacctt aaatgggaag agacttcaga agcaaatctc
    11281 ggaatcgatt ttgctttcct gaacagccgg cttagtggta gtcttgagct ttattacaag
    11341 atcaccaatg acttgcttgg agagtataat gttccggttc cacca
//
LOCUS       DAOQXS010000021        11071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6338_length_11071_cov_3.341322, whole genome shotgun sequence.
ACCESSION   DAOQXS010000021 DAOQXS010000000
VERSION     DAOQXS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_11071_cov_3.341322"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1942
                     /locus_tag="PKM34_01290"
     CDS             206..1942
                     /locus_tag="PKM34_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82243.1"
                     /translation="MKIFSFNQAFPTKGMITFSMSVLFLGVCSGQVSQDEVMKNARAL
                     HHERPEGEYIPSDPATRETSPAYKFFSTGFSVVQANVDNNGNNILNDAANEPSIAFNA
                     QDPDQIAIGWRQFDNINNSFRQAGYAFSTDAGETWTFPGVIDPGVFRSDPVLGYDRNG
                     NLFYNSLTAQDDQYWTNTYKSTDGGETWEMGTFAQGGDKQWMSIDRTSGIGEGNIYEF
                     WNGYYSVCSPYNFTRSTDGNESYENCSTIPGDPYWGTTCVGPDGAVYMCGSYWAGGFM
                     VAKSSNAQDPDDGVIWEQTVQVNLAGENSSNNWNSPNPGGLLGQAIIALDSTGGDFDG
                     NLYLLCSVERNNTSDPRDVMFSRSTDGGWTWSAPVRINDDLGNNAYQWFGTMSVAPDG
                     RIDVVWLDTRNNLGSVNSALYYSYSLDGGVTWTPNEQLSEIFNPHVGWPQQNKMGDYF
                     DMYSDEEGAHLAWAATFNNEQDVYYSVITPDYIGVDEKYGITGITLLQNYPNPFKGST
                     TIRYNLTDNTQVALQIFTTDGREIATLVDGYEQAGSHQVSFDAVNLKNGVYLFKLTTD
                     KSSITKRFTLIN"
     gene            1966..2829
                     /locus_tag="PKM34_01295"
     CDS             1966..2829
                     /locus_tag="PKM34_01295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ82244.1"
                     /translation="MNELIRIAIAYDFDGTLAPGNMQEHSFIPKLGLDKDEFWRESNA
                     MSKEHDMDDILSYMQLMLQKARERNIPVTRRALLEHGRQIRFFSGVESYFEEINRYAG
                     SKNVVIDHHIISSGVRDIIKGTVIAPYFKNIFASGYVFDNKDVAIWPALAINYTNKTQ
                     YLFRINKGIDNSFDNSLINKYVEEERRPIPFSRMIYLGDGETDVPAMKMIKFKGGTAI
                     AVYDESKLQDHGKASAREICEDLIRQNRADFIAPADYSKSSKLFKILQHLINRMVEEE
                     RLLRLKNGDFA"
     gene            3120..4250
                     /locus_tag="PKM34_01300"
     CDS             3120..4250
                     /locus_tag="PKM34_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82245.1"
                     /translation="MIPRQLEEIILAKLFQGKAVIVLGPRQVGKSTLLKLIQQKRDEK
                     VLFLNCDFNDIRKQLTEPGINDLRRLIGDHRLVFIDEAQRVMNIGMTMKLITDQMEGI
                     QLVVTGSSSLDLSNVINEPLTGRKFEYYLYPLSIKEIVDYYGFVETNRRLEQFMIYGT
                     YPDVLNNPGHEAEILSNLVSSYLFKDVLIFQDIRKPEFIEQLLEALALQVSSEVSFNE
                     LAQLLKTDPHTIQRYISLLEKAFIIFRLRSYSRNVRNELKKSRKIYFFDNGVRNAILG
                     NYSSVAGRNDVGMLWENFIIAERMKYLHYNKLYAKGFFWRTTQQQEIDYLEELDGKLT
                     AYEVKWNPLRKVHFPKTFTMNYPETTVHHITRDNFWRHLGVE"
     gene            complement(4440..4934)
                     /locus_tag="PKM34_01305"
     CDS             complement(4440..4934)
                     /locus_tag="PKM34_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ82246.1"
                     /translation="MVDYEIIKQYAREKKISLAELAERIGYTESGFHKAVANNTLKTD
                     KLIKIAEVLSISLESFIKSEDVLRMATALNKNKIELKPDNETFNRDLIESQERMMRDA
                     VIFYREQLKEKDRQIAGKDKQIDHLMNQLELKDGLIRSIIEFARGMGIDLKDSTKVDD
                     NSKG"
     gene            5062..5289
                     /locus_tag="PKM34_01310"
     CDS             5062..5289
                     /locus_tag="PKM34_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82247.1"
                     /translation="MKLKLSVNKRIRTNKRLKFALLDYFNISGSTLQRWLDSNDPSFT
                     EYKSLQLIAAYLHVDDVEELLESADVSSVGI"
     gene            5513..>6676
                     /gene="dnaG"
                     /locus_tag="PKM34_01315"
     CDS             5513..>6676
                     /gene="dnaG"
                     /locus_tag="PKM34_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNQ82248.1"
                     /translation="MIANQVIEKVLDRADIVQVIGEFVDLKKKGQNYQGRSPFTDEKN
                     PSFIVSPSKRIFKCFSSDKSGGVVKFLKEYKGMSFPEAIRFLADKYQVEIVEEKKLTK
                     DDQEHFRVREELFNLNEFAASYFRSALSSSKQAVDYANDRISGDNQTEFNIGYAPGEW
                     HNFQQKASKAGFSLTTMILSGLVSQNESQQNFDFFRNRLIFPIHNHQGRICAFAGRTL
                     DGIQPKYLNITNNPVYNKSFVLYGLFFAQQTIRKLNFSVLVEGYTDVISLHSAGVLNV
                     VAPCGTSLNPEHCSLLARYSKNVVLCYDGDDPGQSAAVRNARICLENDLLPSILQLPR
                     DEDPDSYTRNHPEVDMVKFISSGRVAFPLWYADQLFADAQDDPVLRNEAVKQIT"
     assembly_gap    6677..6686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6687..8649
                     /locus_tag="PKM34_01320"
     CDS             <6687..8649
                     /locus_tag="PKM34_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82249.1"
                     /translation="YGEDSLVAFFVETICKKFKIKQAILLEKIKEAEIQANPPETETA
                     SSLPAHIDPKEFQKWGFYEDHNNYFFDTKDGIKQFSNFVMRPLFHVESSVDAKRIFEL
                     INFLGRKKTIEFDMSEMTSLVNFKRVIEGKGNFLFWGTEPYIMRLKQRWYEETKSCTD
                     IRNLGWQKEGFWAWANGISTEEGFQEIDQTGLVLQNNHYFFIPAFSNIYLDDKSIFLD
                     ERKFIYLERDITLRNWAELFIEVFGVNAYFGIAFYISSLFRDHLLHLFNNFPILNLFG
                     PKGTGKSQMAMSISCLFGRQQTPFNIHNGTKAGLAEHIQQFINAFAWIDEYKNNIEYD
                     KIETLKSIYDAIGRNRLNYDKGKKKETTMVNSAVILSGQEMPTADVALFSRVIFLQFN
                     KTEYSVEEKEVYEKLKALEKQGLSCITADIIRHRAFFIENYYHHYDQVLKDIMADLDD
                     RVVEDRILRNWCAIIASFLTLSSRLDLPFTYETIKSEAVKLILNQNQQIATSNELATF
                     WDLFESLYDSNTLVNNWHFLVTHAYSINTTKEGHKILNRPMQVLKFKFNAVYQVYSEH
                     MRRQGGKALPATTLKYYLEKSKQFLGCEASTKFSLRSYDSAEGKFVDQKQVTTSYCFA
                     YDDLQINLLRTLEMEKPDYSTFNTAEAFD"
     gene            9090..9362
                     /locus_tag="PKM34_01325"
     CDS             9090..9362
                     /locus_tag="PKM34_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82250.1"
                     /translation="MTFIVDEHRIAYFDSKPDCIRLALMEDFHTQGKKHLGMPLVCLS
                     PSNGHYVIYRVTDSTRAGRLQFFIDLNLLFVFENIPESVLHYLNDD"
     gene            9366..9842
                     /locus_tag="PKM34_01330"
     CDS             9366..9842
                     /locus_tag="PKM34_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82251.1"
                     /translation="MAKNPIYVSIEMRPYLVDYTIHFYGEHPIRANEKSKLLQMLSEY
                     LTHPPVNYRPLQPSPRLITFELPYNKILDIRRNNYISPENFSKIQSYFYGKFQYHFFE
                     FMDSRCLHHCMPFKSSIIEFMDDNNISWDRFQYDSLKRMYLRHRVKFRKSQRFLSR"
     gene            9902..10372
                     /locus_tag="PKM34_01335"
     CDS             9902..10372
                     /locus_tag="PKM34_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82252.1"
                     /translation="MTVISKHTGQNIGGISLIEFAFPYEISAFEIFPGYIAKVTIPKP
                     NAWKTLYATPHSFSGGGSSENTPAGTLYTYDFKFRCPKDRPDLIEAFHRIQTTGAIFR
                     ITDANAQKRIYGTRDNPMVSKSKLLLPGEVQGYNGYEISLSVSTADPALLDPSQ"
     gene            10438..>11071
                     /locus_tag="PKM34_01340"
     CDS             10438..>11071
                     /locus_tag="PKM34_01340"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HNQ82253.1"
                     /translation="MAKVPFQNVKSDGKTADIYIYGPIGYHWWDENSWDAASFVSEFN
                     KLAEDHERINIHINSPGGIIDEGLPIFNAIQGCEKDTHTYIDGIAYSMGAVIALAGKT
                     VHAARNSLMLFHNASGWAQGHAEAFRDIAKMLDTYDSSLITSLSSKTGLTEDEVRQKY
                     FDYKDHLLSATEAKTEGFVDVIEDYSAKLPENIAGMTGDQLFALFKNQDSK"
BASE COUNT         3092 a         2452 c         2425 g         3092 t
ORIGIN      
        1 ttaaccacaa aggaacacca aggaattcac aaaggcgcac aaaggtatat taattgatat
       61 tcaactactt agtgttcctt cgtgatgtac tttgtgcgcc ttagtggtta ataaaaagcc
      121 ttttgagaca gcctcttttt cattggttat gatagtatat aaaccggata aatgttaatt
      181 ttacagcgta tcaaaattca atgtcatgaa aattttcagt tttaatcagg ctttcccgac
      241 caaagggatg ataaccttct ccatgtcggt cttattctta ggtgtttgct ccgggcaggt
      301 aagccaggat gaagtaatga agaatgccag ggcgttgcat cacgaacgtc ctgaaggaga
      361 atatattccg tctgatcccg ctacccggga gacatctccg gcttacaaat tcttttcaac
      421 cgggttctct gtggttcagg ccaatgtgga taataatggc aataatattt tgaatgatgc
      481 ggccaatgag ccttccatcg cattcaacgc tcaggatcct gatcagatag ccattggctg
      541 gcggcagttc gacaatatca ataattcctt caggcaggct ggctatgcat tcagcacgga
      601 tgctggcgaa acatggactt ttcccggtgt gattgatccc ggagtattcc ggtcggatcc
      661 ggtactgggt tatgaccgga atggcaattt gttttacaat agcctgacgg cccaggatga
      721 ccaatactgg accaatacct ataaatcgac cgatggggga gagacctggg aaatgggaac
      781 ctttgcccag ggcggggata aacaatggat gtcgatagat aggacctcag gaataggaga
      841 ggggaatatt tacgaattct ggaacgggta ttacagtgtt tgctcaccgt acaactttac
      901 ccggtcgact gacggtaatg aaagctatga gaactgctcc accatccccg gcgatcctta
      961 ctggggaacg acctgcgtcg gacctgatgg cgcggtttat atgtgcggca gctattgggc
     1021 cggtgggttt atggtggcta aatcttccaa tgcacaggat ccggatgatg gcgtgatctg
     1081 ggagcagacg gtacaggtta acctggcagg ggagaacagc agcaacaact ggaattcgcc
     1141 gaatcccgga ggtcttctgg gccaggccat catagcgctt gattcgaccg gaggagactt
     1201 cgatggtaat ctgtatttgt tgtgttcggt ggaacgcaat aatacctcag atccgcgtga
     1261 tgtgatgttc tcacgcagca cagatggtgg ctggacctgg agtgctcccg tcaggatcaa
     1321 tgatgacctg ggcaataacg cttaccagtg gttcggaacg atgtcggttg ccccggatgg
     1381 ccggattgac gtagtatggc tcgatacccg caataacctc gggtcggtga attcggcgct
     1441 ctattattcc tattcgctgg atggcggtgt cacctggacg ccaaacgaac aattgtcgga
     1501 aatattcaat ccccatgtcg gctggcccca gcagaacaaa atgggtgact attttgatat
     1561 gtattcggat gaagagggcg ctcacctggc ctgggccgct acattcaaca atgaacagga
     1621 tgtctattat tcggttatta cacccgatta tattggagtg gatgagaaat acggcataac
     1681 cgggatcact ttgctgcaaa actacccgaa tccgttcaaa gggtcgacaa ccataaggta
     1741 caatttaaca gacaatacgc aggtagcttt acagattttt acaaccgacg ggcgggagat
     1801 tgccacgctg gttgacggct atgagcaggc agggtctcac caggtaagct ttgatgccgt
     1861 taacctgaaa aacggggttt acctgtttaa gctgacgacg gacaaatcat ccattactaa
     1921 aagatttact ttaattaatt agctataatt agttgaataa caattatgaa tgaactgatc
     1981 cggattgcca tagcctacga ctttgacggc acgctggcgc cgggaaacat gcaggagcat
     2041 tcctttattc ccaaacttgg actggataaa gatgagttct ggcgggaatc caatgccatg
     2101 tcgaaggagc acgatatgga cgatattctt tcgtatatgc agcttatgtt gcagaaagcc
     2161 cgggagagga acataccggt gactcgccgg gcgttgctgg agcatggccg tcaaataagg
     2221 tttttcagcg gtgtggaatc ttattttgaa gagatcaacc ggtatgccgg atcgaagaat
     2281 gtggtgatcg atcatcatat catttcttcg ggggtaaggg acattatcaa agggaccgtg
     2341 attgcgccgt atttcaagaa tattttcgcg tcgggttatg tgtttgataa caaagatgtt
     2401 gcgatctggc cggcgctggc gataaactac accaacaaga cccagtattt attccggatc
     2461 aataagggga tcgacaattc tttcgataat tcgttgataa acaaatatgt tgaagaagag
     2521 cggaggccga tcccgttttc caggatgatt tacctgggtg acggggaaac ggatgttccg
     2581 gccatgaaga tgatcaaatt taaaggcggg acggcgattg cagtatacga tgaaagcaaa
     2641 ctccaggatc acgggaaagc ttcagcgcgg gagatctgtg aggacctgat ccggcagaac
     2701 cgggccgatt ttattgcacc cgccgattac tcgaaaagct cgaaactgtt taagattttg
     2761 cagcatttga tcaaccgcat ggtggaagaa gagcggctgc tccggttaaa aaacggggat
     2821 tttgcttgat gctgtcgggt acttccgacg ctgacaggtc aaccttagtc ggacgctgac
     2881 aggtcggcat cctttcaatc ctgaacacgt ccgccggctg gcggacctgt cagcgatgga
     2941 aagaatttcc gatccggtac tggttgcgca aaaatttcat ttctaacccg gcgcgccaga
     3001 tgatcaaatg agaagagtgg agggggtagt aaagtctgaa tcataactca tgattattat
     3061 aatattcctg atctacgttc taaatttatt atctttgtat cgttaaaata tgccgtgata
     3121 tgattccaag acaacttgaa gaaataatcc ttgcaaagct ttttcaggga aaagccgtta
     3181 tagtactggg tccaaggcag gtaggtaaat ccacgctgct taaattgatt cagcaaaaaa
     3241 gggatgagaa ggtgctcttt ctcaattgcg acttcaacga tatcagaaaa caactgacag
     3301 agccaggcat taacgatctt cgaaggttga tcggggacca tcggctggtc tttatcgatg
     3361 aagcccaacg agttatgaat atcggaatga caatgaaact gatcacagat cagatggaag
     3421 gcatacaatt ggtagtgacg ggctcttcgt cactggattt gtcaaatgtc atcaatgaac
     3481 cgctcaccgg cagaaaattc gaatattacc tttatccgtt gtcgattaag gaaatagttg
     3541 attattacgg atttgttgag accaatagga ggctggaaca gtttatgatc tacggaacct
     3601 atcctgatgt tttaaataac ccgggacatg aagcagaaat attaagtaac cttgtcagca
     3661 gttacttgtt caaagatgtg cttattttcc aggatatccg gaagccagaa ttcatcgagc
     3721 aactacttga ggcgcttgcg ctacaggttt cgtcggaggt ttcattcaat gagctggcgc
     3781 agttactgaa gacggatcca cacactattc agaggtatat cagcctgctt gaaaaagcct
     3841 ttatcatttt caggctgagg tcatacagcc gcaatgtaag aaatgaactg aagaaaagcc
     3901 ggaagatcta tttttttgat aatggtgtca ggaatgccat actgggaaat tatagcagtg
     3961 ttgccggcag aaacgatgtt ggcatgctgt gggagaattt catcattgcc gaaaggatga
     4021 aatatcttca ttacaataag ctttatgcaa aagggttttt ctggaggacc actcagcaac
     4081 aggaaattga ttacctggaa gaacttgacg gaaaattgac tgcttatgaa gtcaaatgga
     4141 atccattaag aaaagttcat tttccaaaga catttacaat gaactatcct gaaactacag
     4201 tccatcatat taccagggat aatttctggc gacatcttgg cgtagagtaa tttgtcccat
     4261 aattaatagt atggaaaaca ctacattttt acagttcaat tcgttaccaa caggcttata
     4321 ttactggata aaattcactt ttttagcact cttataatac ggttagctgt cattttgtat
     4381 ctatccaaat ggtcatgttt ttatttcgtg caattgtttg atttattttt acttatcttt
     4441 taacctttcg aattatcatc tactttagta ctgtctttca agtcaattcc cattcccctg
     4501 gcaaattcaa tgatgcttcg gatcaagcca tcctttaatt cgagttgatt cattaaatga
     4561 tcaatctgtt tatccttgcc tgcaatctgc ctgtcttttt cttttaattg ctccctgtag
     4621 aagataactg catctctcat cattctttcc tgtgattcta taaggtccct gttgaaggtt
     4681 tcattatcgg gctttaattc gatcttgttt ttattcagcg cagttgccat tctcaatacg
     4741 tcctcagatt taataaagct ctcaagggat attgaaagga cttcggcaat ctttatcaac
     4801 ttatccgttt tcagggtatt atttgccacc gccttatgaa acccagactc agtatatcct
     4861 attctttcgg ccaattctgc cagtgaaatt ttcttttcac gggcatattg tttaattatt
     4921 tcataatcaa ccatgtcgtt ttttaattgt ctttcttaaa acttaattac agtaaaaggt
     4981 tgtaaataac agtatttagc tgtaattttg tattgtcaaa tttgattacc aaatatagta
     5041 acttttttat taaaatcaag catgaaacta aaattatctg tcaacaaacg aattcggact
     5101 aataagaggt taaaatttgc ccttcttgat tactttaaca tttcaggttc aaccctgcaa
     5161 agatggctgg attcaaacga tccttcattt accgaataca aatccctgca gctgattgcc
     5221 gcatacctcc atgttgatga tgttgaagaa cttcttgaat ctgctgatgt atcatcagtc
     5281 ggaatatgaa gttttcgatc tatatttcta tagaacaaga gcttgagaag gccttacagg
     5341 accttcaatc ttcactacca tcaactaaca cttatgaaaa tcaatcttcc acgccttgcg
     5401 atgatcctat cgcaaggctt actaaaatgt cctatctgct taagttccgg cccggaaatt
     5461 acttttctca tctcaagcct gatctgtcaa ggtttcctta attcaccaag ttatgattgc
     5521 taatcaagta atagagaaag tcttagaccg ggcagacatt gtccaggtga ttggtgaatt
     5581 tgttgatttg aaaaagaagg ggcagaacta ccaggggaga tcccctttca ccgatgaaaa
     5641 gaatccttct ttcatcgttt caccgtccaa gcgcatcttt aaatgctttt cctccgacaa
     5701 atcaggtgga gtggtcaaat tccttaaaga atataaagga atgtcttttc ccgaggcaat
     5761 caggtttctg gccgacaagt accaggttga aatcgtggaa gaaaagaagc tgaccaagga
     5821 cgatcaggag catttccggg ttcgggaaga gttgttcaac ctcaatgaat ttgcggccag
     5881 ttatttccga agtgccctgt cttcgtcaaa acaagccgtt gattacgcca acgatcggat
     5941 atcgggcgat aatcaaacgg aattcaatat cggttacgca cccggagaat ggcataactt
     6001 tcagcagaag gcttccaaag caggcttttc ccttaccacg atgatcttat cgggactagt
     6061 ctcccagaat gaaagccagc agaattttga ttttttccgg aaccggctga tctttcccat
     6121 tcacaaccac cagggcagga tctgcgcttt tgccggtcgt accctggatg gaattcaacc
     6181 aaagtacctt aatatcacca acaacccggt ttataacaaa tcctttgttc tttacgggct
     6241 gttctttgcc cagcaaacca tccgtaaact caacttttct gtgcttgttg aaggatatac
     6301 agatgttatc tcactgcatt cggcaggagt cctgaatgtc gttgcgccct gcggaacttc
     6361 cctgaatcct gagcattgtt ctttacttgc ccgttacagc aagaatgtag ttctatgcta
     6421 tgatggagac gatcctggac agagtgcagc tgtacgcaat gcccgtatat gcctcgaaaa
     6481 tgatctttta ccttctatcc tgcaacttcc gcgggatgaa gatcctgatt catacaccag
     6541 gaatcatccc gaggtcgaca tggtaaaatt tatctcttcg ggccgggttg cttttccatt
     6601 gtggtatgca gatcaattgt tcgccgatgc ccaggatgat cccgtacttc gcaacgaagc
     6661 cgtcaagcag atcactnnnn nnnnnngtac ggggaagatt ccctggttgc attctttgtt
     6721 gaaacgattt gcaaaaaatt caaaatcaag caggccattt tacttgaaaa gatcaaggag
     6781 gcggaaatcc aggcaaatcc tccggagacc gaaacggcca gctcattgcc tgcccacatt
     6841 gatcccaagg aattccagaa atggggtttc tatgaagatc acaacaatta tttctttgat
     6901 actaaagacg ggatcaaaca gttctccaat tttgtcatga ggcccttgtt ccatgtagaa
     6961 agctccgtgg atgccaaacg aatttttgag ctgatcaact tcctgggacg caaaaagacc
     7021 attgaatttg acatgagcga aatgacttct cttgtcaatt tcaaaagagt aatcgaaggc
     7081 aaaggtaact tcctgttctg ggggacggaa ccttatatca tgcgattgaa acagcgatgg
     7141 tatgaagaaa cgaagagttg cacagacata cgtaacctgg gctggcaaaa ggaaggattc
     7201 tgggcatggg ccaacggcat ttcaacggaa gagggcttcc aggagattga tcagaccggt
     7261 cttgtactgc aaaataatca ttatttcttt atcccggcct tttctaacat ttaccttgac
     7321 gacaaatcca ttttcctgga tgaacgcaaa ttcatttact tggaacgtga tatcaccctc
     7381 cgaaattggg ctgagctttt catcgaagta tttggcgtca atgcttattt cgggatagcc
     7441 ttttacattt catccctgtt ccgggatcac ctgctgcatt tgttcaataa ttttccgatc
     7501 cttaatcttt tcggtccaaa gggaaccggc aagagccaga tggccatgtc catatcttgt
     7561 cttttcggca ggcagcagac cccgttcaat atccataacg ggaccaaagc cgggcttgca
     7621 gagcatatcc agcagttcat caatgctttt gcctggatcg acgagtacaa gaacaatatc
     7681 gagtacgaca agatcgaaac tctgaaatcg atctatgatg ccattggccg taaccgtctt
     7741 aattatgaca aaggcaagaa aaaagagacc actatggtca attccgcagt cattctctcc
     7801 ggccaggaaa tgcccacggc agatgtagcc cttttttcca gggtgatctt ccttcaattc
     7861 aacaagaccg aatattccgt cgaggaaaaa gaagtctatg aaaagctcaa ggcactagaa
     7921 aaacagggac tttcctgcat tacggctgac atcatcaggc acagggcttt tttcattgag
     7981 aattattatc atcattatga ccaggtcctt aaagatatca tggctgattt agatgacagg
     8041 gtggtggaag accgcatcct gagaaactgg tgcgctatca ttgcttcgtt cctgaccctt
     8101 tcatccagac ttgacctgcc ttttacctat gaaaccatta aatcggaagc cgtcaagctg
     8161 atcctgaacc agaaccaaca aattgcaacc tccaatgagc ttgccacatt ctgggatctc
     8221 tttgaatcgc tgtacgattc aaatacactg gtcaacaact ggcatttcct ggttactcat
     8281 gcctattcca tcaataccac aaaggaaggc cacaagatac tcaaccgacc gatgcaggtg
     8341 cttaagttta aattcaatgc cgtttaccag gtatattcgg aacatatgag aaggcagggg
     8401 gggaaagccc tgccggccac tacactgaaa tactacctgg aaaaatcaaa gcaattttta
     8461 ggatgtgaag catcgaccaa gttcagcctg agaagctatg acagtgcaga aggcaaattc
     8521 gtggatcaga agcaggtgac cacatcctat tgctttgctt atgatgacct gcaaataaac
     8581 ctgttacgca cactggagat ggaaaagccg gattattcca catttaatac cgccgaagcc
     8641 tttgattaat ctatattttc catacggttc cttatcaaat tcaatacatt attatccaac
     8701 tatggcttat aattgttgtc gccgattttc ttcaatggtc cggcaaagaa attttctttt
     8761 tcagaagatt tcattttccc ggactacccg aagctacaga actactatga tttttattaa
     8821 agtgatattg aatactctat tacattatca attgatcaag taccgaaaat acaggtagtt
     8881 ctatgtagtt tccggtagtt tgcggtaggt gatcattttg agaaaatcag gtctcatttt
     8941 ttcaattttt gagtaataaa ctaccaaaac taccggaaac ttaaatagct atcctgataa
     9001 tcaggtaggt agttcgggta gcgtagtagt ttcaacctat tcccgtttca cttcaaaaca
     9061 gatttattta tccattacca tccagtctca tgacctttat tgttgacgaa caccgaatag
     9121 cttattttga ttcaaaaccg gattgcattc gccttgcact tatggaggat ttccataccc
     9181 aggggaaaaa gcaccttggt atgccgcttg tctgcctttc gccatccaac ggccattatg
     9241 ttatttaccg ggtgaccgat tcgaccaggg caggcaggct gcagttcttc atcgatctta
     9301 atttgctgtt cgttttcgaa aacattcctg aatccgtgct tcattactta aacgatgatt
     9361 aacctatggc aaaaaatccc atttatgttt ccattgagat gcggccctac ttggtggatt
     9421 acacgatcca cttttacggg gagcacccga tcagggccaa tgaaaagtcc aagttgctgc
     9481 agatgctgtc ggagtacctg acccatccgc cggtaaatta caggccgttg caaccatccc
     9541 cgaggctgat cactttcgag cttccataca ataagatcct ggatatccgc cggaacaatt
     9601 acatttcccc ggagaacttt tcaaagatcc agtcttattt ctatggcaag ttccagtatc
     9661 atttctttga attcatggac tccaggtgcc tgcatcactg tatgccgttt aaaagttcga
     9721 ttatcgagtt tatggatgac aacaacattt cctgggaccg gttccagtac gattcgttaa
     9781 aacgaatgta cctgcgccac cgggtaaaat tcagaaaatc gcaaagattt ttgtcacgct
     9841 agcttcctgt ttttacctta tatcccacat gattgatttt taacctttta tattttatcc
     9901 aatgactgta atttcaaaac ataccggcca gaatattggg gggatatccc ttattgaatt
     9961 cgctttcccg tatgaaataa gcgctttcga aatttttccg gggtacattg ccaaggttac
    10021 catcccgaaa ccgaatgcct ggaaaaccct ttacgccaca ccgcattcat tttcaggagg
    10081 cggatcatcg gagaacaccc cggcaggaac cctgtacacg tacgatttta aatttcgttg
    10141 ccccaaagac cggcctgacc ttatcgaggc ctttcaccgg atacaaacca cgggagccat
    10201 ctttcgaatc acggatgcca atgcccagaa gcggatctat ggcacccggg ataacccgat
    10261 ggtttcaaaa tccaaattgc ttttgcccgg tgaagtgcag ggatacaacg gttatgagat
    10321 ctctttgtcg gtctcaacgg ctgatccggc cctcctggac ccgtctcaat agtcctttat
    10381 ccggcctgcg cacctcttta atattgtggt tcgaaaatcg aacagcaata ttaatttatg
    10441 gcaaaggtcc cgtttcaaaa tgtcaagtcc gacggcaaaa ccgccgacat ttacatttat
    10501 ggtccgatag gatatcactg gtgggatgag aacagctggg atgcagccag ctttgtttcc
    10561 gaatttaaca aactagccga ggatcatgag cggatcaaca tccacatcaa ttctcccggg
    10621 gggatcattg atgaaggcct tccgattttc aacgcgatcc agggctgtga aaaggatacc
    10681 catacctaca tagacgggat cgcttattct atgggagccg ttattgcact ggccggcaaa
    10741 accgtccatg cagcccggaa ttcgctcatg ctttttcata atgcatccgg atgggcacag
    10801 ggtcatgccg aagctttccg ggacattgcc aaaatgctcg acacctatga ttccagcctg
    10861 atcaccagcc tgtcatccaa gaccggtctg accgaggatg aagtccgtca gaaatatttc
    10921 gattataagg atcacctgtt gtcggcaacc gaagccaaaa ccgaaggctt tgtcgatgtg
    10981 atcgaagact attccgccaa acttccagag aacattgccg gcatgaccgg ggaccagctc
    11041 tttgccctat tcaagaacca ggattcaaag g
//
LOCUS       DAOQXS010000022        10835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6524_length_10835_cov_3.974212, whole genome shotgun sequence.
ACCESSION   DAOQXS010000022 DAOQXS010000000
VERSION     DAOQXS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_10835_cov_3.974212"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PKM34_01345"
     CDS             complement(<1..1092)
                     /locus_tag="PKM34_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNQ82254.1"
                     /translation="MRWSLNLPETPSGLIEMESQFRIFGKKHPMGILSALSKPYSRHI
                     AGKIRHDSLHAVECQLKMMNMLVERGRTTIYGKARHFNEIVSYEDFRRRIPLTDYEDI
                     RPLIERISTGEKDILWPGRPVYFAKTSGTTSGTKYIPVTRDSISNHINTARNALLVYM
                     DRTKNFAFADHSMIFLQGSPVLDKKGVIPAGRLSGIVAHHVPGYLQKNRMPSWKTNCI
                     DDWETKVDAIVEETLKKRMSLISGIPSWLRMYFEKLVDKAGKPVGEIFPDFSLLVYGG
                     LNYEPYREAFKKLIGRPVGTLELYPASEGFLAFQDEGPGEGLLLNVNSGIFFEFIPVN
                     KMHNDDPERIPLEGVEKNTDYAVVRSNNAG"
     gene            complement(1129..1533)
                     /locus_tag="PKM34_01350"
     CDS             complement(1129..1533)
                     /locus_tag="PKM34_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82255.1"
                     /translation="MIEHVHKHITTELQQNTKTDTIFILTAILLNLIALAVNSGLVED
                     SRTDNSLLIVMYLFVFLIIVVNIVVIIGIMKGKQTRMKLVDGLMRMYKDQGVDKYYDE
                     SLLSNYSLRYNLFIMVVVSTGIIATVVPFVVR"
     gene            complement(1565..2107)
                     /locus_tag="PKM34_01355"
     CDS             complement(1565..2107)
                     /locus_tag="PKM34_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNQ82256.1"
                     /translation="MIRGDDCRMNGSFEHVKNGFPVNWCYYAMETVPDGDFDILIDSV
                     QSKEGKRSLKFQVRTCSSIGGWYSPGFFEEFKVLPGQTYKISFCVLNQGCDFKVGVST
                     GMKGNPGPSETILRTNGKYSDWHYFEYSFTIPLVNDNIRFEANILSPGTILFDDIRIE
                     GLTDKDELTLYPYRGSEKCK"
     gene            complement(2451..2819)
                     /locus_tag="PKM34_01360"
     CDS             complement(2451..2819)
                     /locus_tag="PKM34_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82257.1"
                     /translation="MKKQILRWAPRIIAILAVLFMMIFSMDCFGPGYSLKEQLICFVM
                     HNIPAFIVIAALVIAWKWELIGGILFVLIFLAAGIFFHSFGRNPASLIVITPFLVAGV
                     LFMLDYFLMKDKSEMESHGN"
     gene            3025..3951
                     /locus_tag="PKM34_01365"
     CDS             3025..3951
                     /locus_tag="PKM34_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82258.1"
                     /translation="MQQKENTDFKSLKTRIVATMQQSYPGLSSDISEWKGQDIVNFQE
                     ELLQKVNAHISEKWFYMHMKSGKSTLPRIDVLNLLSRYAGYADWNDFVYRNGGQKPKP
                     AVSSGNRYFIIIPLLVIAILGIFYLVNKLVSNRNYTFCFYDATTREQITHADIEVKII
                     LENESPITYLCSSDGCFTLKTDRSFIKMVVNSPYYRADTINRTLKKFNPQEIIGLQAN
                     DYALMLHYFSEMNTEDWQKRRLKLDEMFDESAMIYQVVNDKSGTGMELYSKWEFIDKL
                     TMPSHSLKNIEILDTKYIGDRIVVLRFRISDK"
     gene            3952..4599
                     /locus_tag="PKM34_01370"
     CDS             3952..4599
                     /locus_tag="PKM34_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82259.1"
                     /translation="MKRLFNILIIIVVYMIFCGKSCDDDGERINRQSHRASLAKDSIL
                     HDFELDFLTEEARYAAGVNAMQKLNDLADYMEIYADRSLEPAFREKAGEMINKMFRSP
                     DNHLFLGPVRKEKMAQITPEEFLKKGFGDDYIRVKINFDSVKVIEPLVLKDEGNYSGK
                     MSAYQSMIGFTANDSLVLPARRVTIDFFSTRQVKTFGSDSIEVRVVRLGDIYLEK"
     gene            4707..5867
                     /locus_tag="PKM34_01375"
     CDS             4707..5867
                     /locus_tag="PKM34_01375"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNQ82260.1"
                     /translation="MKKVIVRTTLFIIIIHTGLFGQNSIRFTFSAVEGSDYFRLDSLR
                     VRNMTQDCDTVLIYPDTVLVINYVGINDSQNSAGGFNMIRNFPNPVTSLSKINLEIPE
                     KDYVNLTLADAAGRILYSASLTLGPGIHRFLFTPPGNGLFIFNASWRGNNCSARILAS
                     GVSQGNEFSLKYMCTSGSSNTLKTRRSIAEYLYNPGDELLLTGYGDGLESGFADSPET
                     DQEYIFQFAANIPCPCLDSLLYDGQWYHTIQVFSQCWMKENLNAGIMIPSSQAQTNNG
                     IIEKYCMINNESYCDILGGLYFWNETMQHVLETGGRGICPEGFHVPTDLEWQILEGAV
                     DSELEIGAPGWNASGWRGTDAGGNLKQTGTDLWEPPNTGGTGAAFSVRCIRN"
     gene            5887..6390
                     /locus_tag="PKM34_01380"
     CDS             5887..6390
                     /locus_tag="PKM34_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82261.1"
                     /translation="MKDKKINTLVCTAQWLAALTGSIYGLVLAIFGYLSGWGPGGSMN
                     GPFWRVLLSAAISILAGAFIGTFVVRKLARLLFEKPFSKLESGLRAFALVLAGSFPAF
                     VSSWLTGYIMGKLTGTIEGLDWITVLVYTPLMSFIYGIPVCFGTDLLFGIFVFFYLKD
                     VDDLRRV"
     gene            complement(6618..8420)
                     /locus_tag="PKM34_01385"
     CDS             complement(6618..8420)
                     /locus_tag="PKM34_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82262.1"
                     /translation="MSQTSYKYPEREFSKKKITKEGMQRLYRLFRFVGPQKYTFIAGM
                     AFLVLSSLTTLVFPMLIGELLDAASSSTLEKINELGMILIGVFLVSAVFSYFRIYLFA
                     VVTQKTLALLRQTTYNHLIKLPMAFFSSRRVGELNSRISADISLLQETFTTTLAQFVR
                     QVITIVGGIVLLTVISPRLTVFMLALIPVAAIVARIFGTYIRKLSKKAQTVVAESNTI
                     VEETLQAVASVKAFANEAFEIMRYKKKTDEVIAISLKGAKWRGLFISFIMFALFGSII
                     GVIWYGVYLIHQGAGLSTGDLLKFVLYSVFIGGSISGVADLYSDIQKSIGATENLLDI
                     LDETPEDLLSETTGSATGITGDVRFENVSFSYPTRKDVAVLKDITFSVRHGGQVALVG
                     PSGAGKSTISALLLRFYDPTGGRILIDGKDARDYDLTALRGNMAIVPQEVLLFGGTIR
                     ENIEYGKPGATKEEILHAAELANAMEFIGKFPEGLETLVGERGVQLSGGQRQRIAIAR
                     AILKNPAILILDEATSSLDAENERLVQEALEKLMEGRTSFVIAHRLSTIHKADQIVVI
                     DQGEVKEMGTHEELLAREGGIYQQLTRLQINVMG"
     gene            complement(8583..9932)
                     /locus_tag="PKM34_01390"
     CDS             complement(8583..9932)
                     /locus_tag="PKM34_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82263.1"
                     /translation="MKKFLFVICLVSLFGMAVKAQENPLKLQGGLQIDHRFRLENGNW
                     SWNENRLELKLDKKFADKARFRSDIWLRSFGFPFLQSAQQLFNKDETSPYNLDFREAY
                     VELYGVFTKNLDMKIGRQRIAWGTADKLNPTDNLNPWDLEDIWDFGRHSGSDAIQMKY
                     YLKDFYLEADYIFYFRPAVLPRGDWAEGLMASPFELPGYLTLGSVSDSLAMPALNLKE
                     NATYAFKFGGFAGGFDFSASYVYGRDGLPLNYYNEISMTETPGKIDVKSYLLFPRQHI
                     IGADLAGAIGAVGIWAEGAAFLPEKEVILTNDLSAFGLPPMDSVVLEKKPWFKYAAGF
                     DYTFRGGHYINVQYLHGFVHERGNGNLNDYLVLSYEKYLFNDKLKINPFTAALVVSDW
                     EKPADNFAWIYMPAITYIPNENTEINLGVRLIGGEGDDVFSKYKDKDEFVLSVRYKF"
     gene            complement(9942..10670)
                     /locus_tag="PKM34_01395"
     CDS             complement(9942..10670)
                     /locus_tag="PKM34_01395"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ82264.1"
                     /translation="MKNVVVIMILGLMVSPVFSQTASEVLRKADDVMYAPKDQKAAVK
                     IILTDKKSNTQERAAQYIQKGSEMRLFKFTAPASQQGIAFLSLPDDVMYLYLPAYEKE
                     RRIASHIKNQTFAGTDFSYDDMESKPLSDEYNAEMIQQTADSWKLKLTPKDPADTEYS
                     RLEVTINKDNNYLRQADYFDRGGVKVKQLINKKIEKTDGYWAATEIEMTDLLKEHKTV
                     FLTIELSFDNNLTAENFTVRELKK"
BASE COUNT         2849 a         2671 c         2525 g         2790 t
ORIGIN      
        1 gccggcatta ttgctgcgaa ccaccgcata atcggtgttt ttttcaaccc cttccagggg
       61 gatgcgctcc ggatcatcat tatgcatctt gttgacgggg ataaattcga agaagatgcc
      121 gctgttgaca ttcaggagca gcccttcacc cggcccttcg tcctggaaag ccaggaatcc
      181 ttccgatgcg gggtagagtt ccagcgtgcc gaccgggcgc ccgatcagtt ttttaaaggc
      241 ctcccggtag ggctcatagt tcagcccgcc ataaacgagc aaggagaaat cggggaaaat
      301 ctccccgacc ggtttgccgg ccttgtcaac gagtttttcg aaatacattc tcaaccagga
      361 tgggatgccg ctgatgagcg acatcctctt cttcagtgtt tcctcaacga ttgcatcgac
      421 tttcgtctcc caatcatcga tgcagttggt tttccaggag ggcatgcggt tcttctgaag
      481 gtaaccgggc acatggtggg ccacgatccc cgaaagccgg cccgccggga tcacaccctt
      541 tttatccagc accgggctgc cctgcagaaa aatcatactg tggtcggcaa aggcaaagtt
      601 ttttgtccgg tccatgtaga ccagcagggc attgcgggcg gtgttgatat gatttgagat
      661 ggagtctcgt gtgacaggga tatactttgt gcctgatgtg gtgccggaag tcttggcaaa
      721 ataaaccggc cgcccgggcc agagaatgtc tttttccccg gtggatatcc tttcgatcaa
      781 tggtcggata tcttcgtaat cagtcagcgg aatgcgcctc cgaaagtctt catagcttac
      841 gatctcattg aaatgccggg ccttaccata gattgtcgtg cgaccacgtt caaccagcat
      901 attcatcatt ttcaactggc actccactgc atgcagggaa tcatgacgga tttttccggc
      961 aatatgccgt gaataaggtt tgctgagagc agagagaatt cccatcgggt gttttttccc
     1021 aaagatacga aattgagact ccatctcgat gagcccagat ggagtctccg gaagattgag
     1081 actccatctc atttactcga gatggagtct cgtttaagca acacaaaatc acctcaccac
     1141 aaacggcact accgtggcta taatgcccgt actcacgaca accatgataa atagattata
     1201 cctgaggcta taattgctca ataacgattc atcatagtat ttatcaactc cctggtcttt
     1261 atacatccgc atcagcccat ccaccagttt catccgggtc tgcttgcctt tcattatgcc
     1321 gattattact acaatattca ccacaatgat caggaataca aataaataca taacgatcag
     1381 gagtgagttg tcggtccttg agtcttctac cagccctgaa ttaacagcca gggcgatcag
     1441 gttgagcagg atcgccgtca ggatgaagat cgtatcggtc ttcgtgttct gctgtaactc
     1501 ggttgtaatg tgtttgtgca cgtgctctat cataatacat tgattttaaa gtttataact
     1561 ttaactattt acatttttcg gatcccctgt acggatacag ggtcaattca tctttatcgg
     1621 tcagcccttc aattctaata tcatcaaaca agatcgtgcc aggcgataat atatttgctt
     1681 caaaccttat attgtcattc accagtggga tggtgaaaga atattcaaaa taatgccaat
     1741 ctgaatactt tccattggtt ctaagaatgg tttcacttgg gcctggattt cctttcatgc
     1801 cagtactgac gccgacttta aaatcacagc cctgatttaa tacacagaag cttattttat
     1861 aagtttggcc gggcaaaact ttaaattcct caaagaatcc gggagaatac cacccgccaa
     1921 tggacgaaca ggttcgcacc tgaaatttca gagatctttt accttctttt gactgaacag
     1981 aatcgataag aatatcaaaa tcaccatccg gtaccgtttc cattgcgtaa taacaccaat
     2041 ttaccgggaa accgtttttt acatgttcaa aactcccatt cattctgcaa tcatctcctc
     2101 ttatcaaaac acttccgggt ttgcaacctt acattgcaat cagtaatatt acacacgata
     2161 tccagcgggc cctttttagg atgatcaggc caggttctgc cttacagata aagaaaaaat
     2221 atttgaatcc tgctataggt aaaaccatgg ccagcgggtt gtcggctatg ccaatagaag
     2281 gcgaggctgt gataaacaga aaagcaatta taaaaaggat cagagtaatc tttgaatgaa
     2341 tggcctcaaa atgtgtggta aaatgatttt gcatactaat aactatttaa atcacagaga
     2401 ctccatctca atttttcgag atggcgtctc gtttggtatg agactccatc tcaattgcca
     2461 tgagactcca tctcactttt atctttcatc agaaaataat ccagcataaa taacaccccc
     2521 gccaccaaaa atggagttat gactatgagt gacgctggat tgcgaccaaa actgtggaaa
     2581 aagattcccg ctgccagaaa gataagcaca aacaatatcc ccccgatcag ctcccacttc
     2641 caggcaatca ccagagcagc aatgacaatg aaagccggta tgttgtgcat cacaaagcag
     2701 atcaattgtt ccttcaagga atatccggga ccaaaacaat ccatcgaaaa gatcatcatg
     2761 aaaaggaccg ccaggatggc gatgatccgt ggggcccagc gtagaatttg ctttttcatc
     2821 ttattatttt taaagattct ggcctgaaaa attgatttga cacaattgac gtcgtaactt
     2881 tacctaaagt taaccaaaat aattctgatc attgatataa aattacatcg ttttcaggga
     2941 gataccatgg aattcctgtt gtaaaaccat tgtaaatcct tttacaagcg ttcttcatcc
     3001 acgtttcatt aatccgtttc atctttgcaa cagaaggaaa ataccgactt taaatcactt
     3061 aagaccagga tcgtggcaac catgcaacaa tcttatccgg ggttaagttc cgatatttcg
     3121 gaatggaaag gccaggatat cgtcaatttc caggaagaac tgcttcagaa agtgaatgcc
     3181 cacatcagcg aaaaatggtt ctacatgcat atgaaatcgg gcaaaagtac cctgccgcga
     3241 atcgatgtgc ttaacctgtt gagcaggtac gccgggtatg ccgactggaa cgattttgta
     3301 tacaggaatg gcggccaaaa accaaaacca gccgtaagca gcggtaaccg ttattttata
     3361 ataattcccc tgcttgtcat tgcaatcctc ggcattttct acctggtcaa taagctggtc
     3421 agcaacagga attatacttt ctgcttctat gatgccacga ccagggaaca aataacccat
     3481 gcagatatcg aggtgaaaat catcctggaa aatgaatcgc caataacata cctgtgcagt
     3541 tccgatgggt gtttcacact gaaaaccgac cggagcttca tcaaaatggt cgtaaattca
     3601 ccctactatc gtgccgatac cataaacagg acgctgaaga agttcaaccc gcaggagatc
     3661 atcggcctcc aggccaacga ttatgcattg atgctgcatt atttttcgga gatgaatacg
     3721 gaggactggc agaaaagacg actgaaactg gatgaaatgt ttgatgaaag cgcgatgatc
     3781 taccaggtcg tgaacgataa aagcggcacc gggatggaac tatacagcaa atgggagttt
     3841 atagataaac tgacgatgcc ttcccattcg ttgaaaaata tcgaaatctt agatacgaag
     3901 tacattggtg accgtattgt tgtactacgg ttcagaatat cggataaata aatgaaaaga
     3961 ttgtttaaca tattgatcat cattgtagta tacatgatct tctgcgggaa gtcgtgcgat
     4021 gacgacggcg aaaggattaa caggcaaagt caccgggcat ctttggcaaa ggacagcatt
     4081 ctccatgatt ttgaattgga ttttttaaca gaagaagccc gttatgccgc cggtgtcaat
     4141 gcaatgcaaa agctgaacga ccttgccgat tatatggaaa tctatgccga tcggtcgctg
     4201 gaacccgcat tcagggagaa agcgggtgaa atgatcaaca agatgtttcg ttcaccggat
     4261 aaccacctgt ttcttggacc ggtcaggaag gagaagatgg cacagataac cccggaggaa
     4321 tttttgaaaa aaggattcgg agatgattat atccgggtga agattaattt tgactctgtc
     4381 aaggttattg agccgctggt cctaaaagat gaaggaaatt attctggcaa gatgtcggct
     4441 taccagtcaa tgattggatt taccgctaat gacagcctgg ttctgccggc caggcgggtg
     4501 acaattgact tcttttcaac ccgccaggtt aaaaccttcg gaagcgactc gatagaagtg
     4561 agggtggtca gactggggga tatttacctg gaaaagtgag actccatctc aatttgcaaa
     4621 ataatttaca aatcctttac aagcgactta cagaagaaac ggtaacgaca gttatatttt
     4681 tgaggcttgt ttttaattca agtcatatga aaaaagtcat tgttcgtacc accttgttta
     4741 taatcatcat ccataccggc ctttttgggc agaattcaat ccgattcaca ttttccgctg
     4801 ttgagggatc agactatttc cggctggata gcctgagggt aaggaatatg actcaggatt
     4861 gcgatactgt gctgatttat cctgatacag ttctggttat taattatgtg ggaatcaacg
     4921 acagccaaaa tagtgctggc ggtttcaaca tgattcggaa cttcccgaac cctgtcacca
     4981 gtctgtctaa gattaatctt gaaatacctg aaaaagatta cgtaaacctg acattggcag
     5041 atgccgccgg gcgcatactg tattcagcct cactgacact gggtcccgga atccatagat
     5101 ttctcttcac gccacccggg aacgggcttt ttatcttcaa tgcttcctgg agagggaaca
     5161 attgctctgc acggatcctg gcttccggtg tcagtcaggg aaacgaattt tcactgaaat
     5221 acatgtgtac gtcaggctct tcgaatacgt tgaaaactcg caggtcaatc gcagaatatc
     5281 tttataatcc cggcgatgag ctcctgctta ccggatatgg cgatggtctt gaatcagggt
     5341 tcgcagattc gcccgaaacc gaccaggaat atattttcca gtttgcggcg aacattccct
     5401 gcccctgcct cgattccctg ctctatgacg ggcagtggta tcacaccatc caggtattca
     5461 gccagtgctg gatgaaggag aacctcaacg ccggcattat gatcccttct tcccaggccc
     5521 agacgaacaa cggcatcatc gaaaagtatt gcatgatcaa taacgagtca tattgtgaca
     5581 tccttggcgg cctttatttc tggaatgaaa ctatgcagca tgttcttgaa acgggaggca
     5641 ggggtatttg ccctgaagga tttcacgtac cgacagacct cgagtggcag atcctggaag
     5701 gagccgtcga cagcgaactt gagatcgggg cacccggctg gaatgcaagt ggctggcgag
     5761 ggacagatgc cgggggtaat cttaaacaaa ccgggacaga tctctgggaa ccaccgaata
     5821 ccggcgggac gggggcagcg ttttcggtga ggtgtatcag gaattaggta ttctaaattt
     5881 gaaaatgtga aggataaaaa aattaatacc ctggtgtgca cagcccaatg gcttgcagca
     5941 ttgaccggca gcatctatgg cctggtgctg gccatttttg gttatctgtc gggttggggc
     6001 ccgggaggaa gcatgaacgg gccattctgg agagtcctgc tgtcggcagc catatcgatc
     6061 ctggcaggag ctttcatcgg tacatttgtc gtcaggaaac tggcacggtt actattcgaa
     6121 aaaccatttt caaagttgga atcgggactt agagcctttg ccctggtcct tgcaggaagt
     6181 ttccctgctt ttgtttccag ctggttaacc gggtacataa tggggaaatt gacgggtacc
     6241 attgaggggc tcgattggat cacagtcttg gtttacactc cgctcatgtc ttttatctat
     6301 ggcatcccgg tatgttttgg aacggatctt ttgtttggca tttttgtatt tttttatttg
     6361 aaagacgttg atgatcttag aagagtatag aactgataaa aaatccgatg aaatatgtat
     6421 tgacttttcc tttatcaggc agtaaatttg agtcggaatt tggttcacgg tagcatgatc
     6481 ctggaacgcc gaaaaccggc cctccggtat ctactaatag caactaacga aacttcgtcc
     6541 accactggcg aactcaggac aggccgtcgg ctgctgaccg gcaatatcgt gaaggtctga
     6601 ccgccgcctg ccctccctca tcccatcaca ttgatctgca accgtgtaag ctgctggtag
     6661 atcccgcctt cgcgggccag caactcctcg tgtgtcccca tctctttcac ttcaccctgg
     6721 tcgatcacca cgatctggtc ggctttgtgg atggtcgaaa gccggtgggc gatcacgaac
     6781 gaggtgcgcc cttccatcag cttctccagc gcctcctgca ccagccgctc attctccgcg
     6841 tcgagcgaac tggttgcttc atccaggatc aggatggcag gatttttcaa tatggcacgg
     6901 gcaatggcta tgcgttgccg ctgaccgcca gagagttgca cgccgcgctc cccgacgagg
     6961 gtttccaacc cttccgggaa cttgccgatg aactccatcg cattggccag ctccgccgca
     7021 tgcaaaatct cttccttcgt ggcgccgggt ttgccgtatt cgatattttc ccggatcgtg
     7081 ccaccgaaca gcaatacctc ctgcggcact atcgccatat tcccccgtaa tgccgtgaga
     7141 tcgtaatccc gggcatcttt cccgtcgatc aggatgcggc cgcccgtggg atcataaaac
     7201 cggagcaaca acgcgctgat ggtgctcttg cccgcccccg acgggccaac cagcgctacc
     7261 tgcccgccgt ggcgcacact gaaggtaatg tctttcagta cagcgacatc cttgcgggtt
     7321 gggtaggaaa aggatacatt ctcaaaccgg acatccccgg taattcccgt tgcgcttccc
     7381 gtagtctccg aaagtaaatc ttccggcgtc tcatcaagga tgtcgagcaa attctccgtc
     7441 gccccgatcg acttctggat atcggaatac aggtcggcca ccccgctgat cgagccgccg
     7501 atgaacaccg aataaagcac gaacttcagc agatcgcccg tcgacagccc cgccccctgg
     7561 tggatcaggt aaacgccgta ccagatcacc ccgatgatcg atccgaacag ggcaaacatg
     7621 atgaaagaga tgaaaagccc gcgccatttg gcccctttca gcgaaatggc gatcacttcg
     7681 tcggtcttct tcttgtatct catgatctcg aaagcctcgt tggcaaaagc cttgacactg
     7741 gcaaccgcct gcagcgtctc ttcaacgatc gtgttcgatt cggctaccac cgtctgtgct
     7801 ttcttcgaca gcttccggat gtaggtcccg aagatcctgg caacaatagc cgccacaggg
     7861 atcagcgcca gcatgaacac cgtcagcctg ggcgaaatga ccgtcagcag cactatgccc
     7921 ccgacaatgg tgatcacctg cctgacaaac tgcgccaggg tggtggtgaa tgtctcctgc
     7981 agcagcgaaa tatccgccga gatgcggctg ttcaattccc cgacccgccg gctcgaaaaa
     8041 aatgccatcg gcagcttgat gagatggttg taggtggtct gccggagcag cgccagggtt
     8101 ttctgggtaa cgacggcaaa caggtagatc cggaaatagg agaaaacggc gcttaccagg
     8161 aaaacaccga tcaggatcat ccccagctcg ttgatttttt cgagggtgga actggatgcg
     8221 gcatccagca attccccgat cagcatcggg aataccagcg tggtcaggct ggaaagcacc
     8281 aggaaggcca ttcccgcaat aaaggtgtat ttctgcgggc ccacaaagcg gaacagccgg
     8341 tataaccgct gcatcccttc ctttgtgatc ttctttttgg aaaattccct ttcgggatat
     8401 ttgtatgacg tctgcgacat aaatttttca ggtatactca ttctttgcag gtttgcgaaa
     8461 gcaaaactgc ttagaatgag ttatgccgag aggaacagca gtataaaatt tgcaaatacc
     8521 gaaaaatcgg tacaggctct gttcctattc ggtataaaca aaattaatag caaatagttt
     8581 tgctaaaact tgtacctgac agaaagaaca aattcatcct tatccttgta ctttgagaac
     8641 acatcatcac cttcaccccc gatcagcctg acccccaggt tgatctcggt gttttcgttg
     8701 gggatatagg tgatggcggg catatagatc cacgcaaagt tatccgcggg tttttcccag
     8761 tcggaaacga ccagggcggc agtgaaagga ttgatcttca gtttgtcgtt aaacagatat
     8821 ttctcgtagc tgagaaccag gtaatcgttc aggttgccgt ttccccgctc atggacaaaa
     8881 ccatgaaggt actgcacatt gatgtaatgc ccgccgcgga atgtgtaatc aaaccctgcc
     8941 gcatatttga accagggctt tttttcgagc accaccgagt ccatcggggg aagcccgaat
     9001 gcggaaagat cgtttgtcag gatgacctcc ttttcgggaa gaaatgccgc cccttcagcc
     9061 cagatgccca cggctccgat ggcgccggcc aggtcggccc cgatgatgtg ctgccggggg
     9121 aaaagcaggt aactctttac atcgatctta cccggggttt cagtcatgct gatctcgtta
     9181 tagtaattca gcggcaatcc atcccggccg taaacataac ttgccgaaaa atcgaacccc
     9241 ccggcaaaac cgccgaattt aaaggcatag gtggcatttt ccttcaggtt gagcgcaggc
     9301 atcgccaggc tatccgaaac ggaaccgagc gtcaggtaac cggggagctc gaacggcgat
     9361 gccatcagcc cttcggccca gtcgccacgg ggcagaacgg ctggccggaa atagaaaatg
     9421 taatcggcct ccaggtaaaa atctttaagg taatacttca tctgaatggc atctgagccc
     9481 gaatgccgtc cgaaatccca gatgtcttcc aggtcccacg gattgagatt gtcggtcgga
     9541 ttcagcttgt cggccgtccc ccaggcaatc ctctgccgcc cgatcttcat atcgaggttt
     9601 ttggtgaaaa ctccgtaaag ctccacatat gcctcacgga aatcgaggtt gtacggagag
     9661 gtttcatcct tgttgaagag ctgttgagcg ctctgcagaa acggaaaacc gaacgaccgg
     9721 agccagatat cggaacggaa ccgggcttta tcggcaaatt tcttgtccag cttcagttcc
     9781 agtctgttct cattccacga ccagttaccg ttttccagcc ggaaccggtg atcgatctga
     9841 agcccgccct gtagtttcag gggattttcc tgggccttga ctgccatccc gaaaagggac
     9901 actaagcaaa tgacaaacag aaactttttc atatgccatc attacttttt caattccctt
     9961 acagtgaagt tctctgctgt aaggttatta tcgaacgata attcaattgt cagaaaaaca
    10021 gtcttatgct ccttcagcag gtcggtcatc tcaatctcgg tagccgccca gtatccgtcg
    10081 gttttttcaa tctttttatt gatcagttgt ttaactttta ctcctccacg gtcaaaataa
    10141 tcggcctggc ggagataatt attgtctttg ttaatagtga cttccagccg tgaatattcg
    10201 gtatcggcgg gatctttggg cgtcagcttc agcttccagg aatcagcagt ctgctggatc
    10261 atctctgcat tgtattcatc tgaaagaggt tttgattcca tatcgtcgta agaaaagtcg
    10321 gtgcccgcaa aggtctggtt cttgatatgc gaggcgatcc tccgctcttt ttcgtaagcc
    10381 ggaagataca ggtacataac atcatctggc agcgacagga aggcgattcc ctgctgcgat
    10441 gccggtgcgg tgaatttaaa caacctcatt tcggagcctt tctgaatata ctgagcggcc
    10501 cgttcctgcg tattgctttt tttatcggtc aggatgatct tcactgccgc tttctggtct
    10561 ttcggagcgt acatcacatc atcggccttc ctcagcactt cggaagccgt ctgtgagaac
    10621 accggcgaaa ccatcaaccc taaaatcata attacaacca cattcttcat aatcaattaa
    10681 aatttaacta ttcactattc tctattccct tttccctgaa aagaatcgta gccggcagca
    10741 atgtcagcgc cgccgcgccg gaaatgatca tcgttacccc gaccagcagg ccgaagtgct
    10801 gcaatgggac caggtttgag aaagtcagga ccagg
//
LOCUS       DAOQXS010000023        10788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6564_length_10788_cov_4.133979, whole genome shotgun sequence.
ACCESSION   DAOQXS010000023 DAOQXS010000000
VERSION     DAOQXS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_10788_cov_4.133979"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKM34_01400"
     CDS             <1..297
                     /locus_tag="PKM34_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNQ82265.1"
                     /translation="KFGTWAFIISRFLPVVRTMMPMLAGASGLNLRKFSFYNILGGVI
                     WIGTLAPLGYFLGKKYPNLMDYSIWFLLGFIIIASTPVITSFLKKRKLKVIKHP"
     gene            379..1293
                     /locus_tag="PKM34_01405"
     CDS             379..1293
                     /locus_tag="PKM34_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNQ82266.1"
                     /translation="MEINIWFWIGFNAFVVLMLALDLGVFHRKAHEVGFKEAMTWSGI
                     WISLALMFNLGIYYYFGKVKAVEFLTGYIIEKSLSVDNIFVFVLIFSSFAVPAKYQHR
                     VLFWGIIGALIMRAVFIFAGVALINKFHWIIYVFGVFLLYTGIKIAINKGTKINVENN
                     RLLNLFRRYFPVTKEYHGSKFITRIEGKTWATPLMMVLILVETTDLIFAVDSIPAILA
                     ITNDPFIVYTSNVFAILGLRSLYFALAGILKYFHYLHYGLAAILVFVGIKMLITDFYK
                     FDPFVSLGIIGFILLTSIVMSIWKPAKE"
     gene            1394..1786
                     /locus_tag="PKM34_01410"
     CDS             1394..1786
                     /locus_tag="PKM34_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNQ82267.1"
                     /translation="MIIGIETYPDTYRQHYNLLHLLDRMILARGIRPGDYFKDPWNVF
                     DFIVVAVCFIPAIGTHYVAVLRLARILSVFRVISIFPKLQLLVGALLIASLRWAISFS
                     SWRLLKYKTSETSDVKAMIAILIKRSTI"
     gene            complement(1842..6209)
                     /locus_tag="PKM34_01415"
     CDS             complement(1842..6209)
                     /locus_tag="PKM34_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ82268.1"
                     /translation="MNKIHSQDECAITFTHGQGFTTTLSSVTANNDDSHTIVLVVAHD
                     GTTGQGYSRMMNYSVEADPGTYSNIIVQPLTGNFSYLNVEPGPSLPGAPFQGFRINRT
                     NNIGPGMPASFSITFTLTGILQDQQVMVKTFSNQFVSLIQDDDFEEVYDCLFPEIIPY
                     YEPPAGGKTSDLIGAELTSLHNTYINNGSYISNDIFQIVDTLVIIDVFALPGQFDSLL
                     AILTSPAMGMIPENSNPDAGVITGLFPIINLLFINELPDKVSYARPVYPALGNAGLVT
                     SQGDTAMITYLARSGFDLSGNGIKIGVISDSYNTQQGNQAGDDVLRGDLPGPANPVYA
                     APVDVVKEFPYGTRSDEGRAMLQIVHDIAPGAELAFRTGFLGAADFAAGIHELHQNGC
                     NIIVDDITYISEPFFRDGIVARAVDSVKSLGVTYISAAGNFGTKSYESAFYPVPAPDG
                     ITGMAHNFAGGGNGTDIYQSISLTPGNYTVVLQWDDGTPENFTATDFDIYLTKENGAT
                     LFGFNRVNTGGAPIEVLPFTVLGDNVLSNLMIVRASGSGPAFLKYIIFRGDIQINEYA
                     TASPTLVGQANAAGSIAVGAVLYSNTPEYGVNPPTIASFSSRGGTPVNGVNRNKPDIS
                     APNGVNTSVNLGGVNIDGDAFPNFFGTSAAAPHAAGLAALLMEASQKYRSAALSPGEM
                     KSILQSTALDMGTPGYDAASGAGFIHAGAALGTIANPAPLITGIYYDTALVPGKDTLE
                     VTVTGRLLTAGAAIYINGQPAAGEQVLLGDTAIRSVILPFDERYPMIQVYNPPMAGTN
                     GTDGGLSNPLYFSTKETIVMAIDDKFKKYGEVLPDFTAQFFVEGINGTVPLDSAALTQ
                     PEKARITGIQMATVANNMSNTGLWAIEADPGDPLNPESGLDISDPLDLAILERFDIDI
                     ENGLLQIEKMDLVIYPNDTAFVYGDSINGFSFGFHLGQVHDSAYSIFIEPSDSLAILS
                     SLRSNYATALVNGTALVNATALVNEYGEPLLDATALVNKSMMISNATFSRFATALVNG
                     TFIGAPSFLTATALVNVVSFSGSSALAPATALVNGDVVVNGFSTGGATALVNAGQLIN
                     AFAGATALVNTTTVNGNNNSDAIVILGEDDILILSGDSTGGVDLRGISLITGNTVGQH
                     YIVPGSFLSNNFNITYGLGELTILPDTVTVTADMKVIKAGDPLPSFTATFSGFHDGDD
                     QSVVDTLYFTLSPAYNGAAGTYQIQPHATSANYIFVPVTAPLYVNPYGSGAKQITLSI
                     PCIEVLNPPDTSGFTYLARFTYQNTNTTDVYIPYGPDNMFSGSGSWNGINQPVLFKAG
                     GGSFTVPFNGVLLSYTVKSYKNTTKTTSMARVSSQNGLCLKSEEVKEEEAGDNLQESI
                     KAYPNPTNGKVWIRLDGESVSREQVSLVDLYGKVYPVDVSVVSKELIELDLGNYKAGV
                     YLVRIRTDEETETIRVIRK"
     gene            complement(6547..8691)
                     /locus_tag="PKM34_01420"
     CDS             complement(6547..8691)
                     /locus_tag="PKM34_01420"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNQ82269.1"
                     /translation="MNRKKLLTGVFCVTCLLINAQWSWLNPLPQGHNILDAWFFSQDS
                     GIMVGSGGTIMNTHDGGNSWSIQSISNYEESCYLYDLSFADRNCGCLIDTWKNVWKTS
                     DGGSTWVRIYKDEDDNDIRSVAFRDNTHGFIAGSEGLLLVSDDGGISWQQNGNMPDEY
                     FTKISFITPDSGYILSWQGSIFNTQDGGMTWNLQSSGVWTPLEDLLFINSQLGYVYGQ
                     NGVLLKTTNGGDEWNLCFPQDSSLSIYTLCGTGADTLIMIAEKDMGWYMDLRFERSFD
                     GGQSWLEIPYDNDDPWPYVSLYLGDGTCFFAGGHGLMIKTTDCGNSWTYLSTLITPNL
                     ISDIDFPTNEVGYASMLNYYYPTTDISVLKTIDGGDSWFVLDSLFESQVFYSVEFINE
                     NVGFIGGLNIYMTLDGGQSWTKRYSSDWERVITSIDFPNTALGIAVGQQGLILRTTNM
                     GFTWNAVESGTNFDLLSVDMPDPDTGYAAGGGIFLKTTDGGENWNPVPVSFTDLAEIC
                     FLNTRMGYVTCTEDIMKTTDGGLTWQNITPYNVTEYFRHVSFFDSDTGYAGGGRHFTS
                     SVLMKTTNGGINWAKESVPTGYPIECIYVNQANKVFASSHDLYFFGKSNDGMTGISPS
                     LPGLNTMGATCYPNPFCSSTTITYYLSSRAKVKLSVTDVKGDPVCELFEGYQDAGTHE
                     VIFRAGHLKPGVYFYQLLINNENFTGKFVKIK"
     gene            8902..>10788
                     /locus_tag="PKM34_01425"
     CDS             8902..>10788
                     /locus_tag="PKM34_01425"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNQ82270.1"
                     /translation="MNNSFFYILLAVSFISLNTYSQSSCCTYSLTGQDPGPGEAGETF
                     GIGLGDIDNDGDLDAVIIDAYDDMEVYKNNGAGIFTYHQTYGSGNSWFGAYLVDVDGD
                     TDLDIVVSAFYSGSGCEVWENDGSGNFTFAQGGIASSIAMHQITILDLNGDDAPDIFA
                     PAYSGSGGQVWFNDGTGTFVNTNQSLQAGSATQAAMADFDGDGDPDAYVATTNGSPDK
                     VFLNNGSGFFTPTAQELGNAFSSGAAASDVDGDGDMDVVVANWQVPSQVWLNDGNGTF
                     SPGPQIDNDNYAKAVEISDIDYDCDDDVILGSYGSNGLQVWTNDGLGNFTLCFENTNE
                     IYAHGIAVGDMNGDLMPDVWVGNFSSTSGDYIFLKSTPEFVYDTIQICGGDSVYIGCA
                     WRYSDGDFLQAVNCDTLCWYHVFNTPINVSVTLDNTVLSAIPGYDAYQWFDCNTMLPV
                     SGAISHIFNPEITGTYAVAISHSGCADTSACTFVQTPLVDFAGNPTYGMPPLTVNFTD
                     LSTDMVDTWEWDFGDGNTSGQQNPTHVYTEGGQYSVRLKITGPGGSDSLVKQNYIEVL
                     FNPPTAEFSGTPTTGFAPLEVQFTDLSVDSVNSWLWFFGDGGVAFEQNPSHTYETAGS
                     YTVTL"
BASE COUNT         2748 a         2642 c         2541 g         2857 t
ORIGIN      
        1 aaattcggca catgggcctt tattatcagc cggttcctgc cggtcgtcag gacaatgatg
       61 cccatgctgg ccggcgcttc cggtttgaat ctcaggaaat tttcatttta taacatcctg
      121 ggaggtgtca tttggatagg tactctggct ccgttgggct atttccttgg taaaaagtat
      181 ccaaacctta tggattactc gatctggttt ttgctcggat tcatcatcat cgccagcaca
      241 cctgtcatca catcattcct gaaaaaacgc aagctgaaag ttattaaaca tccttaaaaa
      301 gggtcttttt tgatgtttta aacaattatc ctgtagaact gttttagcat taccttttat
      361 cttcataata accaaactat ggaaatcaat atctggttct ggatcggatt caatgccttt
      421 gtcgtcctga tgctggcact cgaccttggc gtttttcacc gaaaagccca tgaagtgggc
      481 tttaaagaag ctatgacctg gagtggtatc tggatcagcc tggctttgat gttcaacctc
      541 gggatctatt actattttgg gaaggtcaaa gcggtggagt tcctgacagg ttacattatt
      601 gaaaagtctc tcagtgtcga caatatattt gtctttgttt taatattcag ctcgttcgca
      661 gtccctgcaa aataccagca ccgggtcctg ttctggggta tcatcggcgc cctgataatg
      721 cgcgctgttt ttatcttcgc cggggtggcc ctgatcaaca agtttcactg gatcatctac
      781 gttttcgggg tgtttctcct ttatacggga attaagatcg cgattaacaa agggaccaag
      841 atcaacgttg aaaacaaccg ccttctgaac ctgttccgcc ggtattttcc cgttacgaaa
      901 gaatatcacg gcagtaaatt cattacccgt atcgaaggta agacatgggc aacgcccctg
      961 atgatggtac tgatactggt tgaaacaacc gacctgatct tcgctgtcga ctccataccg
     1021 gctatcctgg ccatcacaaa cgatccgttc attgtttaca cttccaatgt ctttgccatt
     1081 ctggggctcc gctcactcta ttttgccctt gcaggcatcc tgaaatattt tcattacctg
     1141 cattacggtc tggcagctat ccttgtgttt gtcgggatta agatgcttat caccgacttt
     1201 tataaattcg atccctttgt ttcccttgga attatcgggt tcatcctgct gaccagcatt
     1261 gtgatgtcaa tatggaaacc ggcaaaagaa tagtttaacc tcctgaatga tgaatataac
     1321 caaaccactg agtgttttca ccgaatatgc ctacttcagg tatttcatcc cgggagtgat
     1381 catttagtcg gccgtgatca tcggtattga gacttaccct gatacatacc ggcaacacta
     1441 taatctcctg catcttctgg atagaatgat cctggccagg ggcattcgac ccggggacta
     1501 ttttaaagac ccatggaatg tgttcgactt catcgtcgtg gcagtgtgct ttatcccggc
     1561 tatcggcact cattatgttg ctgtattgcg attagcccgt atattaagtg tttttcgcgt
     1621 tatatccatc tttcctaaac ttcaactcct ggtgggtgcc ctgctgatag catcccttcg
     1681 atgggctata tcgttctcct cctggcgttt attgaaatat aagaccagtg agacgtcaga
     1741 tgtaaaagcc atgatcgcga tcttgataaa aagatcgacg atctgaaggc gaatcggtca
     1801 atacttaatg ataatcttta atagtcgcga tcccggactc attacttcct gatgaccctt
     1861 atcgtttcgg tttcctcatc tgttctgatc ctcaccaggt aaactcccgc cttgtaattg
     1921 ccgagatcca gttctatcaa ctccttcgat accactgaaa catccacggg ataaaccttt
     1981 ccgtataaat caaccaggct gacctgctcc cggctcaccg attcaccatc caggcggatc
     2041 catacttttc cattggtggg attaggatag gctttgatgg attcttgcaa attatccccg
     2101 gcttcctctt ctttaacttc ttccgattta agacacaggc cgttctgtga actgactctt
     2161 gccattgagg ttgtctttgt agtgttctta taacttttaa cggtataaga cagcaataca
     2221 ccgttgaaag gcacagtgaa cgatccgcca ccggctttga acaacacagg ctgattgatc
     2281 ccgttccagg atcccgatcc ggaaaacata ttatccggac catatggaat gtaaacatcc
     2341 gttgtattag tgttctgata agtaaaccgg gccaggtaag taaatccgct cgtatcgggg
     2401 ggattcagca cttcgatgca gggaatgctt aaagtgatct gctttgctcc cgaaccgtaa
     2461 ggatttacat atagcggagc cgtgacgggc acaaatatat aattggccga ggtggcatgc
     2521 ggctgtatct ggtatgtgcc tgccgcccca ttgtaagccg gactcagggt aaaatacagt
     2581 gtgtcgacaa cagactgatc atcgccatca tggaatccac tgaatgtagc tgtaaatgaa
     2641 ggcagagggt ctccggcttt gatcactttc atatcggcgg taaccgtaac ggtatccggt
     2701 aaaatcgtta attcgcccaa cccgtaagta atgttgaagt tattcgagag gaaggatcca
     2761 ggtacgatat aatgctgccc gaccgtgtta ccggtgatca ggcttattcc ccgcaaatca
     2821 acacctccgg tagaatcgcc ggacaggatc aggatatcgt cttcgccaag tatgacgatg
     2881 gcatcactgt tattgttgcc gttgactgtg gttgtattga ccagggctgt ggcgccggca
     2941 aaggcattta tcagctggcc ggcattgacc agtgccgtgg cgccgcctgt actgaatccg
     3001 ttcaccacga catcgccatt taccagcgct gtggcaggtg cgagggctga actgcctgaa
     3061 aagctgacca cattgaccag cgcagtggct gtcaaaaagg aaggtgctcc gataaaggtc
     3121 ccgttgacca gtgctgttgc aaaccggctg aaggtagcat tggatatcat catgctttta
     3181 ttgaccagcg ccgtggcatc caggagcggt tcaccgtatt cgttgaccag cgctgtagca
     3241 ttgaccagcg ctgtaccgtt aaccagcgcc gtagcataat tggatctcag cgacgacaaa
     3301 atggccaggc tgtcggatgg ctcaatgaaa atggaatagg cagaatcgtg cacctgaccc
     3361 agatgaaacc cgaaactgaa cccgttgatc gaatcgccat atacaaaagc ggtatcgttc
     3421 gggtatatga ccaggtccat tttttcgatc tggagcagcc cgttttcaat gtcgatgtcg
     3481 aatctttcga ggatggccag atcgagcggg tcagaaatat caagcccgct ttcgggattc
     3541 agcggatcac ccggatcggc ttcaatagcc caaaggccgg tgttactcat attgttcgcc
     3601 actgttgcca tctgaatacc cgttattctt gccttctccg gctgggtcaa cgcagcagag
     3661 tcgaggggca cggtcccatt gatcccctcg acaaagaact gtgccgtaaa atcgggtaat
     3721 acctccccgt attttttaaa tttatcgtca atagccatga caatagtctc ttttgtcgaa
     3781 aaatacaagg gattcgacaa accgccatct gttccgtttg tcccagccat cggcgggtta
     3841 taaacctgga tcatcggata ccgttcgtcg aaagggagaa ttaccgaacg gatggccgta
     3901 tcacccagca gaacctgctc gccggctgcc ggctgcccgt tgatataaat ggccgcgcct
     3961 gcggtcagca acctgccggt taccgttacc tccagggtgt cttttccggg tacaagagct
     4021 gtatcgtaat aaatccccgt gataagaggg gcaggattgg ctatcgtccc cagggcagca
     4081 ccggcatgga tgaaaccggc cccggaagct gcatcgtatc cgggagtgcc catatccaga
     4141 gcggtagact gcaggatgga tttcatctcg ccgggcgaca atgcggcaga cctgtatttc
     4201 tgcgaggctt ccatcagaag ggcggccagg ccggctgcat gtggcgccgc agccgaagtg
     4261 ccgaagaaat tggggaaagc gtcgccgtcg atgttcaccc cgcccaggtt caccgaagta
     4321 ttcacgccgt tcggtgcgct gatatcgggt ttattgcggt tcaccccgtt caccggcgtc
     4381 ccgccgcggg atgaaaagga agcgatggtc ggcggattga cgccatattc aggggtgttg
     4441 ctataaagaa cggctcctac ggctatcgat ccggcagcat tggcctgacc gaccagcgtc
     4501 ggcgatgcgg ttgcatattc gttgatctga atgtcgccgc ggaaaatgat atatttcaga
     4561 aacgcaggcc ccgaaccgga agcgcggacg atcatcaggt tgctgaggac attatcgccc
     4621 agaacggtga acgggaggac ttcaataggt gcgccaccgg tattgacccg gttaaatcca
     4681 aacagggtgg ccccgttctc tttggtcagg tatatgtcaa aatcggtggc ggtaaaattt
     4741 tccggagtac catcgtccca ttgcagaaca acagtataat ttcccggcgt cagggagatg
     4801 ctctggtaaa tatcggtgcc gttgccacca ccggcgaaat tgtgggccat gccggttatg
     4861 ccgtcgggag ccggcaccgg ataaaacgcg ctctcataag atttagtgcc gaaatttccg
     4921 gcagcggata tgtaagtcac tccaagcgat tttacgctgt cgacagcccg ggccacgatg
     4981 ccatcacgaa aaaatggctc gctgatatag gtgatatcat cgacgatgat attgcatccg
     5041 ttttgatgaa gttcatgaat gcccgcggca aaatcggcag cgccaaggaa accggttcga
     5101 aacgcgagtt cagcgcccgg cgctatatcg tgcacgatct ggagcatggc gcgcccttca
     5161 tccgacctcg tcccgtaagg aaattcctta acaacgtcga caggtgccgc ataaaccgga
     5221 ttggcaggcc cggggagatc gcctctcaga acatcatcgc cggcctggtt cccctgttgc
     5281 gtgttgtaac tgtcggaaat cacgccaatc ttgatcccgt tgccgctcag gtcaaatccc
     5341 gaccgggcca ggtaagtgat catcgccgta tcgccctgag aagtgaccaa gccggcgttg
     5401 cccaatgcgg gataaactgg gcgggcgtag ctgactttat caggtaattc attgataaaa
     5461 agcaggttaa tgataggaaa aagaccggtt atcacgcccg catccggatt gctgttttca
     5521 gggatcatcc ccatggccgg tgaggtaagt atggccagga gagaatcgaa ttgccccggc
     5581 agggcaaata catcgataat gaccaacgta tcgacgatct ggaaaatatc gttggaaata
     5641 taggagccgt tattgatata tgtattgtga aggctggtga gctctgcgcc gataaggtca
     5701 ctggtcttgc cccctgcagg cggttcataa tacggaatga tctccgggaa caaacagtca
     5761 taaacctctt caaaatcatc atcttgtata agactgacga actggttgct gaaagttttc
     5821 accatcacct gctgatcctg gagaatcccg gtcagggtaa aagtgattga gaaagaggcg
     5881 ggcattccgg gcccgatatt gttggtccgg ttgatcctga acccctggaa aggcgctccg
     5941 gggagcgacg gaccgggttc aacattcagg taactgaaat tgcctgtgag cggctgaacg
     6001 atgatattgc tgtacgttcc cggatcggcc tccaccgaat aattcatcat ccgcgaatat
     6061 ccttgtccgg tcgtaccgtc gtgggcaacg accagcacga tcgtatgact gtcatcattg
     6121 ttggcggtta ccgacgataa agttgtggta aatccctggc catgcgtgaa agtgatcgca
     6181 cattcatcct gcgaatggat cttattcaag aagagcatgt taaagagcaa ggcacaggtc
     6241 agggccacta gaactttgga tctattgcgt ttggttatca tataaagcgg ttctggaata
     6301 tttttattta gaaagtgtct aaacaaagat aaggcgggag ttgtctttac aatattaatt
     6361 aacaatcaat taacaaaata gaccgcgtgg taagtgattg ggtaaagctc gctattggtc
     6421 agcttctaaa aagtgtttcc acttcagact ctggcaggtt tacattaaaa atgaattggc
     6481 agaaatcctg gcgacctgga tgaactgaaa attgaacata gaaattggaa attataaata
     6541 gatgattcat ttaattttta caaatttccc ggtaaagttc tcattattaa ttaaaagctg
     6601 ataaaaataa actcccggtt tcaaatggcc tgccctgaag attacctcgt gtgtacctgc
     6661 atcctggtaa ccttcaaata attcacatac cggatcccct ttgacatctg taaccgataa
     6721 ttttaccttc gccctgcttg ataaatagta agttatcgtt gtcgaactac aaaaaggatt
     6781 aggataacac gtggctccca tcgtatttaa accaggcaat gatggggata ttcctgtcat
     6841 cccatcattc gattttccaa aaaaatatag atcatggctt gaagcgaaca ccttattcgc
     6901 ctgattaaca taaatacatt cgattggata acctgtagga acgctttctt tggcccagtt
     6961 aatcccgcca ttcgttgttt tcataagcac gctgctggta aaatgtctgc ctcctcctgc
     7021 atacccggtg tcgctgtcaa aaaagctaac atgccggaaa tactctgtga cattataggg
     7081 ggtaatgttt tgccatgtca atcccccgtc tgtagttttc atgatatcct ccgtacaggt
     7141 cacataaccc attctcgtat tcagaaaaca aatttctgct aaatcggtaa aggaaacggg
     7201 cacaggattc cagttttctc cgccatcagt ggtttttaga aatattcctc ctccggcagc
     7261 atagccggta tcaggatcag gcatatcgac agataagaga tcaaaattgg ttccggactc
     7321 aacggcattc caggtgaagc ccatgtttgt cgttcgcaat attagtcctt gctggccaac
     7381 ggcaattcca agggctgtat tcgggaagtc gatagaggtg atcacccttt cccaatccga
     7441 ggaatagcgt tttgtccagc tttgaccgcc gtcaagagtc atataaatat taaggcctcc
     7501 tataaatcct acgttctcat taataaattc gacagaataa aaaacctggc tctcgaacaa
     7561 ggaatcgagc acgaaccatg aatctcctcc atcgatggtt ttcagaacac tgatatccgt
     7621 ggtgggatag taataattga gcatggaagc atatccaacc tcatttgtcg ggaaatcaat
     7681 atcactgata agatttggag taatcaatgt tgaaagatag gtccaggaat ttccgcaatc
     7741 agttgttttg atcatcaaac catggcctcc ggcaaaaaag caggtaccat cgccaaggta
     7801 aagggataca taaggccatg gatcatcatt atcataagga atttcaagcc agctttgccc
     7861 tccgtcaaat gatctttcaa agcgcagatc catataccat cccatatcct tttcggcaat
     7921 catgatcagc gtgtcggcgc ctgtaccaca taatgtgtat attgatagcg acgaatcctg
     7981 cggaaagcaa aggttccatt catcgccgcc atttgttgtt tttagtaaaa ctccattttg
     8041 accatataca taacctaact gagaattgat aaaaagcagg tcctccaggg gagtccaaac
     8101 acctgaggat tgcaggttcc atgtcattcc tccgtcttgg gtattaaaaa tcgagccctg
     8161 ccagctcaaa atatatccgc tatcaggagt aataaagctg atctttgtaa aatattcatc
     8221 cggcatatta ccgttctgct gccagctaat tcctccatca tcacttacca gtaacagccc
     8281 ttcggatcct gcgataaaac catgtgtatt atccctgaaa gccacacttc tgatatcatt
     8341 atcatcttca tccttatata tacggaccca ggtactacca ccatcagaag tcttccagac
     8401 atttttccag gtatcaatta aacatccaca atttcggtct gcgaaagaaa gatcatacaa
     8461 atagcaggat tcttcataat tactgatgga ctgaatagac catgaatttc ctccatcatg
     8521 ggtattcatg attgttccac cgcttcccac cataatcccg gagtcctgag agaaaaacca
     8581 ggcatctaat atattatggc cctgaggcaa aggattaagc cacgaccatt gtgcatttat
     8641 gagcaggcaa gttacacaga aaacgccagt taggagtttc tttctgttca tcaggacgct
     8701 gacaaattaa gcataaagtt ataaacttta attacttttt gtcataatta agtgaatata
     8761 atcccagaat tgctattcat tccttcaggg actctcaggc ttcttataac tacaaaggct
     8821 aacgatgtaa ttgatatttt ttctattttc atcgggatta tcagcaatga tgccctatta
     8881 ataaataaaa tgtatatgat catgaataac tcattctttt acattctgtt agccgtatct
     8941 ttcattagcc tgaacacgta ctcgcagagc tcctgctgca cctattctct gaccggccag
     9001 gatccgggac ccggtgaagc aggcgaaacg ttcggtatcg ggctgggcga tatcgataac
     9061 gacggcgacc tggatgccgt gatcatcgat gcttacgacg acatggaggt ttataagaac
     9121 aacggagccg gtatctttac gtatcatcaa acctatggct ccggtaactc ctggtttggc
     9181 gcttacctgg tggatgtcga cggcgatacg gacctcgata tcgtggtttc ggctttctat
     9241 tccggctcgg gatgcgaggt ctgggagaac gacggcagcg gcaactttac cttcgcccag
     9301 ggtggaatag cctcatccat cgccatgcac cagatcacta tcctcgacct gaacggcgat
     9361 gacgcacccg acatctttgc tccggcttac agcgggagcg gcgggcaggt ctggttcaac
     9421 gacggcaccg gcacttttgt caatacgaac caatcgttgc aggccggctc ggctacccag
     9481 gccgcgatgg cggatttcga cggcgatggc gaccccgacg cctatgtcgc caccaccaac
     9541 ggctcgcccg ataaagtgtt cttaaacaac ggctcgggat tttttacacc tactgctcag
     9601 gaactgggaa acgccttttc atccggcgct gctgcatcgg atgtcgacgg cgacggggat
     9661 atggacgttg tggtggccaa ctggcaggtg ccctcgcagg tatggcttaa cgatggaaat
     9721 ggcacctttt cacccggccc ccagatagat aatgataact atgcaaaagc ggtggagatc
     9781 agtgatatcg attatgattg cgatgatgat gtcatactgg gctcctatgg ctcgaacggg
     9841 ttacaggtat ggacaaacga cgggctgggg aattttaccc tgtgttttga aaatacgaac
     9901 gaaatatacg ctcatggcat tgccgtcggg gatatgaacg gtgacctgat gccggatgtc
     9961 tgggtcggca acttcagctc aacttcaggc gattatattt tcctgaaatc gaccccggaa
    10021 tttgtttatg ataccatcca gatctgcggt ggcgacagtg tctacatcgg ctgtgcctgg
    10081 cgatattccg acggcgactt cttgcaggcc gtcaattgcg ataccctttg ctggtatcat
    10141 gtgttcaaca ccccgatcaa tgtgtctgta acgcttgata atactgtcct ttcggctata
    10201 ccgggctatg atgcttacca atggtttgat tgcaacacca tgttaccggt ttcaggagct
    10261 atcagccata tttttaatcc tgagatcacc gggacctatg ccgtagcgat ctcgcactcg
    10321 ggttgcgccg acacctcggc ctgcaccttt gttcaaacgc ctcttgttga ttttgcaggt
    10381 aacccgactt acggaatgcc tcccctgacg gttaacttta ccgatctctc gacagatatg
    10441 gtcgatacct gggagtggga tttcggtgat ggcaatactt ccggccagca aaatccgacc
    10501 catgtctaca ccgaaggggg acaatattcg gtccggttga aaattacagg tcccggtggc
    10561 agtgacagcc tggtgaaaca aaattacatc gaggtgttat tcaatccccc aactgcggaa
    10621 ttctcaggaa ctccgaccac cggttttgcc ccgctggaag tgcagtttac cgatctttcg
    10681 gtggatagtg tgaatagctg gctatggttc ttcggcgacg gcggggttgc ttttgaacag
    10741 aatccgtcac acacctacga aaccgccggt tcgtatacgg tcaccctg
//
LOCUS       DAOQXS010000024        10774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6583_length_10774_cov_3.832727, whole genome shotgun sequence.
ACCESSION   DAOQXS010000024 DAOQXS010000000
VERSION     DAOQXS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_10774_cov_3.832727"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..606
                     /locus_tag="PKM34_01430"
     CDS             256..606
                     /locus_tag="PKM34_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82271.1"
                     /translation="MKTAILTAAMMILMTAANARTIADTNYYQGSPVKTFQASVIKTG
                     PVMIRFSVLNPENDKVILKVYNEDQVKVYQKTVRKSNVTNIGCDMSNAEKGRYTFVVI
                     RNGKEEVKKSIILF"
     gene            700..1740
                     /locus_tag="PKM34_01435"
     CDS             700..1740
                     /locus_tag="PKM34_01435"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ82272.1"
                     /translation="MTVSTTPGILRKWYYRILTGAVTGAGTWFLAEVIFNIKYRYYDL
                     VSMRISYVYAFIFGILILETYFRAGGFLDRILPWGKGPVKRFWAQLLAGFITAFWWII
                     VVRVGLNILLYPGQLIILEDEVIIFSLIMFLMLIVCILQLGYFYNESYRQSLAEMERY
                     RKENAEYQFEMLKLQLNPHFLFNSLNTLSSLVYDNADRAAEFVRKLSEVYRYVLDNRN
                     KELVLLKEEMEFIHAFTFLQGLRFQGMIDFRFGIDPESMGKKIAPMTLQLLIENAVKH
                     NVASRRNPLTISVKAGNGRLSVHNNLQPKEEQPGTGVGLKNIINRYQFLTVEKVEITR
                     EKEYFEVIIPLI"
     gene            1745..2515
                     /locus_tag="PKM34_01440"
     CDS             1745..2515
                     /locus_tag="PKM34_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ82273.1"
                     /translation="MKILIIEDEPFARAELIRLLKATGREFEILEQIDTVEDSIEWLS
                     SHPEPELVFLDIQLADGISFDIFKKVNISSPVIFTTAYDEYAIRAFQLNSIDYLLKPV
                     NPEALAGALDKLDRIRMAAPEPVQSWRENPHLENLLKMLSKEFKSRILLKTGDQIRSV
                     QIEEIAYFYAEDDVVFAMQKNKSRAIVDYTLNQLETMLDPGRFHRLTRSCIAQISSIK
                     KVSKYFNSRLLVELDPPSEEKVLVSRVNVKEVLRWMGE"
     gene            2645..3313
                     /locus_tag="PKM34_01445"
     CDS             2645..3313
                     /locus_tag="PKM34_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM20 domain-containing protein"
                     /protein_id="HNQ82274.1"
                     /translation="MISGLLLLILSGCYDMTIRLEKLPDNTPPTEPIYISGNFNNWDP
                     GDEDYIMQRNSDSVLEISLPKGIGEVQYKFTRGDWSTVEKDPCGFEISNRFAYYGKQE
                     VVHDTVRSWNDLPKPGCPSITIIIDTLPENTPENAVLYLASNINDWDPGSRYWMFIKG
                     PDGKYYIKVPLPNSMESEYKVTRGDWSKVEVDTDGNDIDNRSFSGNAGDEIRIKIARW
                     KDRE"
     gene            3547..4734
                     /locus_tag="PKM34_01450"
     CDS             3547..4734
                     /locus_tag="PKM34_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ82275.1"
                     /translation="MERLFNLSRIKIISVPERFKRFLISEVDWSQRLIGIKGARGTGK
                     TTLLLQQLKNLSPAEAVYVSLDDIYFGENHLVHFAESFARNGGKYLFLDEVHKYPNWS
                     QEIKNIYDSLPELNIVFTSSSALEIQKGKYDLSRRALIYELPGLSFREFLMLKYEMDL
                     PAITLDQILARHEADIFNHQPGFKPYKYFAEYLQHGYYPYFAGDEVHYHARLNETINQ
                     VLESDLPAIFNINYEAIIKLKKLLFILSAIVPYKPNINKLAQQIGSTRDTLLRYLYFL
                     HHAHIIRWLSNESEGINFLNKPDKLYLENTNIEFALGKQLVKSGTIRETFFLNQLSVN
                     HKLAYPEKGDFLVDNKYTFEIGGRKKGNRQILGIEEAYIVSDEIEFGSLNKLPLWIFG
                     FLY"
     gene            complement(4841..5830)
                     /locus_tag="PKM34_01455"
     CDS             complement(4841..5830)
                     /locus_tag="PKM34_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ82276.1"
                     /translation="MPKTQKSNYTSPASSTWEGIRAAFARQQKVGSPGPDDRLDGKTV
                     LVDGASSGLGFAVATQMAMLGARVIMACRSGIPEKGEEVKRRTGNKNVEMVYVDFSDM
                     DSIRNLIATLKEEYAPFDIVVSNAAMVPAQSRKTPQGLEEMFMVNYLSKFILINGLIN
                     EGCLRRKRDGVPPPRIIFVSSESHRHPAAFDWEGFGIYKTYGMQKTVEYYGYYKLLLT
                     TFARELSRRLNNKGKTEISVFALCPGPVNSGIAREAPGIFQPLMKLVFAMFFKSPKKA
                     AEPVMYLAVSKDQEGKAFDYLFKMTRKDIDEKAEDEENGKRLWKMSENIVKTL"
     gene            complement(5850..7178)
                     /locus_tag="PKM34_01460"
     CDS             complement(5850..7178)
                     /locus_tag="PKM34_01460"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNQ82277.1"
                     /translation="MKKDRIIKSSHYGHRPWWFRVLNRAWRSAGKAGFKPDLSKDSLI
                     RAARKSTGLTDLGKDFMDEPLDRLLWSIREEARLHPVGAFITRQRMINLLAARLRAEE
                     MFRKNHGILDRPLLPAWVILGLQRTGTTKLHRLLAADESNRVLLSWEAINPVPLEIRN
                     SKLETWNLEPETRHLERETRNPKQDKRVAIAKKSENALRLMAPGFFAIHPVEHNAPEE
                     DILLLDITFLSTTPEATMNVPSYAAWLETTDQGPAYEYMVKLLKLLQWQRPGKRWILK
                     SPHHLEFINLIDKHFDDVHFFWTHREVTECIPSFISMAAHSRVIFSDEAADGNTIARH
                     WIRKTGYMLRKGLEYRTNNQHKNQFTDIYYDGLVGNSAGILTGIYSLDGGMTPELLEK
                     FSRAEKNNPMRKYGIHEYRLEDFGVRKEDILNETVDYRNFIKNRIRDISR"
     gene            complement(7165..8361)
                     /locus_tag="PKM34_01465"
     CDS             complement(7165..8361)
                     /locus_tag="PKM34_01465"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HNQ82278.1"
                     /translation="MGKLLFLILSIIKNFRKLKLRLTGNTEFGISSSRVVSGKAWDDF
                     CDQLKLAGASLHYPGAPTDPFQQAEGIRYLTRLTRAGLEAFVEYNDPAFPVLRQMVHE
                     TVKMGADNPDNVYLNAQISGHYEYRITGKRNTIDYIGFFTQNGNYGSTGGLAPCGKID
                     DKQLVCEPDGSFEIILSKTPKGKNWLKIENETGLVIIRQTYLDRKTEIPAELRIENLD
                     GRKAPDPVTPEKIDEGLKTAAMFVGGASLLFSRWANNFRKHSNRLPMFDPAVSNAAGG
                     DESIIYYHSHWKLGPEEALVIEVLPPSCDTWNFQLNNYWMESLDYRYFNICLNKASAK
                     YEEDGSVRLIVSPTDPGHPNWIDTCGHTEGTMCWRWYRLKEGVQPVAPGCQVVQFKDL
                     KDHEKR"
     gene            8424..9227
                     /locus_tag="PKM34_01470"
     CDS             8424..9227
                     /locus_tag="PKM34_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ82279.1"
                     /translation="MTDFRRQFGQWGLVAGAAEGIGAAFCEELARRGMNVILVDILKS
                     EMESRAAKLESEFQVKTISKVIDLGSENAASLCMEAIRETGCRLLIYNAAYSRVKPFL
                     SAEAGELDRYIDVNARTPLSLAYLFSGWLKSEGNSGGILLMSSLAGLWGTGLVAAYSG
                     TKGFNLLLAEAISRELRHLNIRVSVCVAGATATPGYLGTHPHYGLIRPSVMKPGKVAS
                     YALKRLVKRTVIIPGMMNRMTYFLLTRIFPRSVSVRLMNRTMERTYRVE"
     gene            9343..9594
                     /locus_tag="PKM34_01475"
     CDS             9343..9594
                     /locus_tag="PKM34_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82280.1"
                     /translation="MTLICKNNQGCRLVQTDVVVPDKAQKENYIRTYCESDDNWKQCQ
                     RYIIRKALWICPDFVLPDSNLTEDEVIERYEQSKAVEKK"
     gene            9652..9969
                     /locus_tag="PKM34_01480"
     CDS             9652..9969
                     /locus_tag="PKM34_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNQ82281.1"
                     /translation="MPTMEIEGRVFEVDGDGFLAHPEIWDKGVAELFARYDGIDPMTE
                     NHWAVVNIIRRNFEEKGMAPMIRTICQETGLRLRDVYELFPLGPARGACRVAGLPKPD
                     GCV"
     gene            9978..10667
                     /locus_tag="PKM34_01485"
     CDS             9978..10667
                     /locus_tag="PKM34_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82282.1"
                     /translation="MEFAWFQWMALSALAICLSVLTFHLIRLIRQGSPQDYSKSAGSI
                     SRGVRYAFTGAMSPARKASAYLHLPTYTAGLIYHSGTFLSFFLFFFIITGNFPEGWLT
                     LLIVSFLAISALCGIGIFIKRMVKQELLSLSNPDDFISNLLVTIFQVFTVIILLCNTN
                     LISEGTTVQLPGCPTAQLIYFFHFTLLMLYVPVGKLRHLLYFFAARYHLGFFYGRRGV
                     WPSGNINSKAG"
BASE COUNT         2781 a         2494 c         2559 g         2940 t
ORIGIN      
        1 tttcgttttc ataagatgaa tagttagtat ttatgcaaag ttaagttcaa aatgtagaaa
       61 ttgtattcca ggagaagcaa aatcagacga aaaacccatg gttgaagttt aattcacctg
      121 aaacgaaatt cactttacct gtcagacgac tcagttcacc cttccgatca tgcagttcac
      181 catcttttca tttccttccc cttcatggcg ggatatcttt gtttcatcaa ttaacaattt
      241 aaaaacacaa aaaccatgaa aacagcaatc ttaaccgccg ccatgatgat cctgatgacc
      301 gccgccaatg cgcgtaccat cgccgacacc aattactatc aggggtctcc ggtaaaaaca
      361 ttccaggcat cagttataaa aaccggtcct gtgatgatca gattcagcgt tctgaatccc
      421 gaaaatgaca aagtgatcct gaaggtctac aatgaagacc aggtaaaagt ttatcagaaa
      481 actgtccgca aatcgaatgt taccaatatc ggatgcgata tgtcgaatgc cgaaaaaggc
      541 aggtacacct ttgtggtcat aagaaacggc aaggaagagg taaagaagag cattattctc
      601 ttctgagacc gccattccga tgaattatac cgttctttct catgttgaaa atttgcctac
      661 ttttgattgg tttggaagaa ttaatccagg gagcataaaa tgactgtcag tacgactccg
      721 ggtattttgc gaaaatggta ttaccgtatc ctgacgggtg cagtgaccgg agcgggcacc
      781 tggttccttg ccgaagtgat attcaatatc aaataccggt actatgacct ggtatccatg
      841 cggatcagct atgtttatgc ttttatattc gggatcctaa ttttagaaac ctattttcgt
      901 gcgggaggat ttcttgacag aattctcccc tggggaaaag ggccggttaa aaggttctgg
      961 gcacagcttc tggctggctt tatcactgcg ttttggtgga taatcgtggt cagggtgggg
     1021 ttgaatatcc ttctctaccc cggccaattg atcatcctgg aggatgaagt gatcattttt
     1081 tcattgatta tgtttttgat gctcatagtc tgcatactgc aactgggcta cttttacaat
     1141 gaaagctacc ggcagtcgct tgctgagatg gaaagatacc gaaaggaaaa tgctgaatac
     1201 cagtttgaga tgctgaaact ccagcttaac ccgcatttcc tttttaacag cctgaatacc
     1261 ctgtcatcgc tggtttatga caatgccgac cgggctgctg aatttgtcag aaagctttcg
     1321 gaagtttacc ggtacgtcct tgataaccgg aacaaggagc tggttttgct gaaggaggag
     1381 atggaattca ttcatgcttt tacttttctg caaggattgc gatttcaggg gatgatcgat
     1441 ttccggttcg gaatcgatcc cgaatcaatg ggaaagaaga tcgcccccat gacgctgcaa
     1501 ctcctgatag aaaatgccgt taaacacaat gtggcctccc gtcgcaatcc actgaccatt
     1561 tcggtgaaag ccggaaacgg ccgcctgtcg gtccacaaca atcttcagcc gaaagaagag
     1621 cagccgggga caggggtcgg cctgaaaaat ataatcaacc gttatcaatt tctgaccgtt
     1681 gaaaaagtgg aaatcacccg tgaaaaagaa tattttgaag tgatcatccc gttaatctga
     1741 aataatgaaa atacttatta ttgaagatga gcccttcgcc agggctgaac tgatcaggct
     1801 gctgaaagcc accggcaggg aatttgagat actggaacag atcgacacag tagaggacag
     1861 catcgaatgg ctgagctccc atcccgaacc cgaactggtt tttctggata ttcagctggc
     1921 cgacgggatc agctttgata tctttaaaaa agtcaacatc tcatccccgg tcatatttac
     1981 gacagcctac gatgaatacg ctatccgcgc tttccagctc aacagcatcg attacctgct
     2041 caaacccgtc aatcccgaag ccctggccgg cgcactcgac aagctcgacc ggataaggat
     2101 ggccgccccg gagccggtac aatcatggag ggagaatccc catctggaga accttcttaa
     2161 aatgctcagc aaggagttca aatcgaggat cctgttaaag acgggcgatc agatccgttc
     2221 ggttcagatc gaggaaattg catactttta cgcggaagat gatgtggttt ttgccatgca
     2281 gaagaacaag tcgagggcta tcgtggatta taccctcaac cagctggaaa ctatgcttga
     2341 tcccggccgt tttcatcgcc tgacaaggag ttgcattgcc cagatatcat ccatcaaaaa
     2401 agtgagcaaa tatttcaaca gccggctttt ggtagagctg gatccgcctt cggaagaaaa
     2461 agtgctggta agccgcgtga atgtgaaaga ggtgctgagg tggatgggag agtgattaat
     2521 ttggcgattt agcgatttgg cgatttgacg atttggcgat ttggcgattt gacgatttgg
     2581 ggatttaatt tggaatttgg aatttggaat tttaatgtca gtacatagaa taaatataag
     2641 ccttttgatt tcaggattgc tgctgctgat cctgtcgggt tgttatgata tgacgatccg
     2701 gctggagaag ttgccggata atacgcctcc gacagaacct atatacattt ccggcaattt
     2761 taacaactgg gacccgggtg atgaggatta tatcatgcaa agaaacagcg actcggtcct
     2821 ggagatcagt ctccccaaag ggattggcga ggttcagtac aagttcaccc ggggcgactg
     2881 gagcacagtg gaaaaagacc cgtgcggttt cgagatctca aaccgtttcg cctactatgg
     2941 aaaacaggag gtggtccacg atacggtaag gagctggaat gacctgccaa aacccggctg
     3001 tccgagcatt accataatta ttgatactct tcctgaaaac actcctgaaa atgccgttct
     3061 ttaccttgcc tctaacatca acgactggga tccgggaagt cgttactgga tgttcattaa
     3121 aggacctgac ggcaagtatt atataaaagt gccactgcca aacagcatgg aatcggaata
     3181 taaagtcacc cgcggagatt ggagtaaagt ggaagtcgat accgacggta atgatattga
     3241 caaccgttcg ttcagcggca atgcggggga tgagatccgg atcaaaattg ccaggtggaa
     3301 agaccgggag taggggatca tatatttcgt atatttagga ctttgatcaa tcctaatacg
     3361 ccatgtcaaa aaaattaatc gtgattgcat tttatgcatt gataagtgtt ggattatata
     3421 cccagcgtcc ttccacggaa ctctcctgcc atccggagag tcaatagaaa cgttaattca
     3481 aaatattgaa ttcacttctc aataagtgtt tattatatta tatttgtcct ttctaaaagc
     3541 ttttttatgg aaagactgtt caatttatcc cgcatcaaaa taatatctgt gccggaaagg
     3601 ttcaaacgtt tcctgatcag cgaagtggat tggtcacaac gccttatcgg aattaaaggg
     3661 gcaagaggaa caggaaaaac aaccctgctt cttcagcaac ttaaaaatct atcccctgca
     3721 gaagcagttt atgtaagcct cgatgacatt tattttgggg aaaaccacct cgttcacttc
     3781 gcggagagct ttgcgaggaa cggcggaaaa tacttgttcc tggatgaggt ccataagtac
     3841 ccgaactggt cgcaggaaat taaaaatatc tatgattctc ttccggaact gaatatcgtt
     3901 tttacaagtt catcagcatt ggaaatccaa aagggcaaat atgatctgag ccggcgtgca
     3961 ctgatctatg agcttccagg actatccttc agggaatttc tgatgctcaa atatgaaatg
     4021 gatcttccgg caattacgct tgatcagatc cttgccagac atgaagcgga catatttaat
     4081 caccagccgg gattcaaacc atataaatat tttgctgaat acctgcagca tggctactat
     4141 ccttatttcg ccggagatga agttcactac catgcccgcc tgaatgaaac cataaaccag
     4201 gttctcgaaa gtgatttacc tgcaatcttc aacattaatt atgaggcgat catcaagctt
     4261 aaaaaactac ttttcatcct gtctgccata gtgccctata agccgaatat aaacaaactg
     4321 gcacaacaaa tcggctcgac aagagatacc ttgcttcgct acctttattt tctacatcat
     4381 gcacatatta tccgctggtt atcaaatgaa tctgaaggca ttaattttct caataagcct
     4441 gacaaacttt atcttgaaaa cactaatata gaatttgctc tggggaaaca actggtaaaa
     4501 tccgggacaa taagagaaac ttttttcctg aaccaattat cggtaaacca taaactggct
     4561 tatcccgaaa agggcgattt cctggtcgac aacaaatata cctttgaaat aggtggccgg
     4621 aaaaaaggta atagacagat attgggcatt gaagaggcat atattgtttc tgatgagatc
     4681 gaattcggct cactgaataa actgccgcta tggatttttg gatttttgta ctaaagccga
     4741 agtcggtctt cgaatagagc cttaccaatt cctaaatttc ctgttcgaat aattcaccgg
     4801 cccgctttcc acttcccgga tgccaccgga tctttccggt ctaaagcgtc ttaacgatat
     4861 tctccgacat tttccaaagc ctcttcccat tctcctcatc ttcagccttt tcatcgatat
     4921 ccttcctggt cattttaaaa agataatcaa acgccttccc ttcctggtct tttgatacgg
     4981 ccaggtacat gaccggctcg gctgccttct tcggtgattt gaagaacatg gcaaagacaa
     5041 gcttcatcaa aggctggaat atgcccggcg cttcccgggc gatccctgag tttaccgggc
     5101 cggggcacag ggcaaaaacg gagatttcgg ttttgccttt gttgttcagt ctccgggaaa
     5161 gttcgcgggc aaacgttgtc aacagcaatt tgtaataacc gtaatattcg actgtttttt
     5221 gcatcccgta tgttttgtaa atgccaaatc cttcccagtc gaatgcagcc ggatgacggt
     5281 ggctttcgga tgatacaaag atgatccgtg gaggagggac gccatccctt ttccgacgca
     5341 gacagccctc atttatcaaa ccattgatta aaatgaattt ggaaagataa ttcaccatga
     5401 acatctcttc cagcccctgc ggtgttttac ggctctgcgc cggcaccatc gcggcgttgc
     5461 tgacgacgat atcgaacggg gcgtattctt ccttcagcgt tgcaatgaga ttcctaattg
     5521 aatccatatc ggaaaaatcc acgtagacca tctctacatt cttgtttcct gttctccttt
     5581 tcacctcttc acccttctcc ggaatcccgc tccggcaggc catgatcacc cgggcaccga
     5641 gcatcgccat ctgcgtagcc actgcaaatc caagccctga gctggccccg tcaacgagga
     5701 cagtcttccc gtcaaggcgg tcgtcaggac ctggcgagcc gactttctgc tgtcttgcaa
     5761 atgcagccct gatcccttcc caggttgaac tggcggggct tgtgtaattc gatttttgtg
     5821 tttttggcat ggaaaaaatt agttataaat cagcgagaaa tatctcgaat gcgattctta
     5881 ataaaatttc tatagtcaac agtttcattc aggatatcct ctttcctgac cccgaaatcc
     5941 tccagcctgt attcatggat cccgtatttg cgcatcggat tgttcttttc tgcccgggag
     6001 aatttttcca gcagttccgg tgtcatcccg ccatcgaggc tgtaaatacc tgtaaggatc
     6061 ccggcagagt tgccgaccag gccgtcataa tagatatcgg tgaattgatt cttatgctgg
     6121 ttatttgtcc tgtattccaa tccttttcgc aacatatacc ctgtttttct gatccaatgg
     6181 cgggcaatgg tatttccgtc ggcagcctcg tcgctgaaaa taacccggct gtgcgctgcc
     6241 atgctgataa acgacgggat gcattcggtc acttcccggt gggtccagaa gaaatgaaca
     6301 tcatcgaaat gtttatcgat cagattgata aactcaagat gatgtggcga tttcaggatc
     6361 catcgcttgc ccggcctttg ccattgcagc agttttagca atttgaccat gtactcatac
     6421 gccgggccct ggtcggtcgt ttcgagccag gcggcgtagc tcggcacatt catggtcgcc
     6481 tccggcgttg tgctcaggaa agtgatgtcg agcagcagga tatcctcttc cggtgcgtta
     6541 tgctccaccg ggtggatcgc aaaaaaaccc ggcgccatta accgcaaagc attttcactt
     6601 tttttggcaa tagcaacacg tttatcctgt ttcgggttcc gggtttcgcg ttccaggtgt
     6661 cgagtttcag gttccaggtt ccaggtttcg agtttcgaat ttcgaatttc taatggtacc
     6721 gggtttatcg cttcccagct taaaaggacc ctgttgcttt catcggctgc aagaagacga
     6781 tgaagttttg tcgtgcctgt cctttggagt cccaggatga cccatgctgg caacaaaggc
     6841 cggtcgagga tgccgtgatt tttcctgaac atctcttcag ccctgagtcg ggcggccagc
     6901 aagttgatca tccgttgccg ggtaatgaac gcaccgacgg gatggagccg tgcttcttcc
     6961 cggatcgacc acaacaaacg gtcgagcggt tcatccataa aatctttgcc caggtcggtc
     7021 agaccggtac tttttcttgc cgctctgatg aggctgtctt tgctgaggtc cggtttgaag
     7081 cccgctttgc cggccgatct ccaggcccgg ttcaataccc tgaaccacca gggcctgtgt
     7141 ccgtagtgcg acgatttgat gatcctatct tttttcatgg tctttcaaat ccttgaattg
     7201 tacaacctgg caaccgggcg ctaccggctg aaccccttct ttcaggcggt accagcgcca
     7261 gcacatcgtc ccttcggtgt ggccgcaggt atcgatccag ttcggatggc cgggatcggt
     7321 gggcgaaacg atcagcctga ccgatccatc ctcttcatat tttgccgatg ctttattcag
     7381 acaaatatta aaataccggt aatcaagtga ttccatccag taattattca actggaagtt
     7441 ccaggtgtcg caggaaggcg gcagcacttc gatgacgagc gcttcttccg ggcccagttt
     7501 ccagtgacta tgataataga tgatgctctc atcgccaccg gcggcattgg aaacggcggg
     7561 atcgaacatc ggcagcctgt tggagtgttt ccggaagtta ttggcccacc ggctgaacag
     7621 caacgaagcg cccccgacga acatggcggc tgtcttcagc ccttcgtcga tcttttcggg
     7681 cgtaaccggg tccggcgctt ttctgccgtc aaggttttcg atccggagtt cggcgggaat
     7741 ttccgttttc cggtccagat aagtttgcct tatgatgacc agtcctgtct cattttcaat
     7801 tttaagccag tttttcccct ttggcgtttt actgaggatg atctcgaagc tgccgtcggg
     7861 ctcgcaaaca agttgcttgt catcgatttt tccgcagggg gccagtccgc cggttgagcc
     7921 gtaattcccg ttttgggtga aaaaaccgat ataatcgatg gtgtttcgtt tcccggtgat
     7981 ccggtattcg taatgtccgc tgatctgggc attgagatag acattgtcgg gattatcggc
     8041 acccatttta accgtttcat gcaccatttg gcggaggacc gggaaggcgg ggtcgttgta
     8101 ttcgacgaac gcttcgaggc cggctcgggt aaggcgggtc aggtagcgaa taccttccgc
     8161 ttgctggaaa gggtcggtgg gtgctccggg atagtggagg gatgcccctg caagcttgag
     8221 ctgatcgcag aaatcatccc aggcttttcc tgagaccaca cgcgatgagg aaataccgaa
     8281 ttctgtatta ccggttaacc tcagctttaa cttcctgaaa ttctttatga tcgataaaat
     8341 taagaagagc aatttcccca ttctgatcag aatttgttat cattgcacgg cactatagtg
     8401 gtgcgcaaat ataaaaaata atcatgactg actttcgtcg acaatttggt cagtggggat
     8461 tggttgccgg ggctgcggaa ggcattggcg cggctttttg cgaggagttg gcccgaaggg
     8521 ggatgaatgt gatcctggtg gatatcctga aaagtgagat ggagtctcgt gctgcaaaac
     8581 ttgaatcgga gtttcaggtc aaaaccatca gcaaagtgat cgatctcggg tcggaaaatg
     8641 ctgcatcact atgcatggag gccatcaggg agaccggttg ccgcctgttg atctataatg
     8701 cggcatacag ccgggtcaag ccttttctct ctgcggaagc cggtgagctc gaccggtata
     8761 tcgatgtgaa tgcaagaacg ccactcagtc ttgcttactt gttttcagga tggctcaaaa
     8821 gcgaaggcaa ttcgggaggg atcctgttga tgtcgtcgct tgcgggatta tggggaacag
     8881 gactggtggc ggcttattcc gggacaaaag gattcaacct gctgttagca gaagccattt
     8941 cgagggagct ccgtcatttg aacatccgcg tgagcgtttg tgttgcgggg gctaccgcta
     9001 cacccggtta cctgggaacc catccgcatt atggtcttat aaggccttct gtgatgaaac
     9061 ccggcaaagt tgcatcgtat gcattgaaaa gactggtcaa aagaactgta atcattcccg
     9121 ggatgatgaa ccggatgact tattttcttc tcaccaggat atttccaaga agcgtgtcgg
     9181 tgagactgat gaaccggacg atggaaagaa cataccgggt agaatgatag ggtatctgaa
     9241 attaatctca aactaaatta ctcttctaac aattgaactc ttgaacaatt gatcgtttga
     9301 acaacctttt tttataattt cggctcctga taaatgataa taatgacttt gatttgtaaa
     9361 aacaatcagg gatgcaggct ggttcaaacc gatgttgttg tacccgataa ggcccaaaaa
     9421 gaaaattata ttagaaccta ttgtgaatca gatgataact ggaagcaatg ccagcgatat
     9481 attatccgaa aagctctctg gatttgcccc gattttgtcc tgcccgattc caacctgaca
     9541 gaagacgaag tcattgaaag gtatgaacag agcaaggccg ttgaaaaaaa ataacattca
     9601 gtccgaaata ttaattaaaa attaaacact caaaactcaa aattctaaag tatgccaacg
     9661 atggaaattg aaggacgggt ctttgaggtc gacggggatg gttttctggc ccacccggag
     9721 atctgggaca aaggggttgc cgagctattt gcccggtacg acgggattga tccgatgaca
     9781 gagaaccact gggctgtggt gaatattatc cggaggaatt tcgaagagaa ggggatggct
     9841 cctatgatcc ggacgatttg ccaggaaacg gggctgcggt taagagatgt ttatgaattg
     9901 tttccgttgg ggccagcccg cggcgcctgt cgcgttgccg gacttcccaa accggatggc
     9961 tgcgtttgat cttatctatg gaatttgcct ggtttcaatg gatggcatta tctgccctgg
    10021 cgatttgctt atctgtcctg acttttcacc tgatccgcct gatccggcag ggtagccctc
    10081 aggattattc caagtctgca ggaagcattt cccgcggggt ccggtacgcc ttcaccgggg
    10141 ccatgagccc ggcgcgaaaa gcctcggcat accttcatct ccccacttac acggcaggct
    10201 tgatctatca ttccggtact tttctctctt tcttcctgtt cttcttcatt ataaccggta
    10261 attttccgga gggttggctg accttgctta tcgtatcttt tctggccatt tctgcattgt
    10321 gtggtatcgg tattttcatt aaaaggatgg tcaaacagga attgttgtca ttgtcaaatc
    10381 ccgatgattt tatttcaaac cttctcgtaa ctattttcca ggtatttaca gtaattattc
    10441 ttctctgtaa tactaacctt atatcagagg gaacgactgt ccaactgccc ggctgcccaa
    10501 ctgcccaact gatttacttt ttccacttca cactcctgat gctgtacgtc ccggttggga
    10561 aactgcggca tttgctttac ttttttgctg caaggtatca cctgggattt ttttacgggc
    10621 ggagaggtgt atggccctcc ggtaatatca acagcaaagc cggttgagat gaaagaagac
    10681 atgaagagca aggcgctcag caaattgaat tctaatggaa gcagccggtt gctgacccag
    10741 ttgaacagct gtgtacattg cggtttatgc gccg
//
LOCUS       DAOQXS010000025        10746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6601_length_10746_cov_3.638855, whole genome shotgun sequence.
ACCESSION   DAOQXS010000025 DAOQXS010000000
VERSION     DAOQXS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_10746_cov_3.638855"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKM34_01490"
     CDS             <1..607
                     /locus_tag="PKM34_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82283.1"
                     /translation="YQYKNDHRDFKSYPLKGYYADFGLVKNGLWTFPEKTINSIQITA
                     TYRKYWELNPRIYLATGINGYFSAGRQPYFVLGGIGYDRDIVRAYEYYLIDAEHFVIL
                     KNNVKFALIPNREHRFQFIRAEKFARIFYALYLNLFVDAGYGYYSQEFGQTTNDLQNS
                     MLIGYGAGLDFVTYYDVVVRLDFSVNYRNEAGFYLHFRAPI"
     gene            667..1545
                     /locus_tag="PKM34_01495"
     CDS             667..1545
                     /locus_tag="PKM34_01495"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNQ82284.1"
                     /translation="MVIALFGKPISQENLKYVQSVIDKLLLKKCRVLFHRQFYESLAG
                     KVETGENIGFFDDMEDLAGKVDYFISIGGDGTILDAVTLVRDSGIPIIGINIGRLGFL
                     SSIGKDEILPAIDKVLDGDFRIDSRTLVALSQPKDLFGDLSYALNELTITRKDTTSLI
                     VLHVYIDDKYLNSYWADGLIIATPTGSTAYSLSSGGPILTPGSRNFVLTPIAPHNLTV
                     RPVVIPDMSSIRVIVEGREDQFLVSLDSRKATIHSNFELMVEKAAFSINMIQMREKDF
                     YMTIREKLKWGLDARN"
     gene            1599..2402
                     /locus_tag="PKM34_01500"
     CDS             1599..2402
                     /locus_tag="PKM34_01500"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNQ82285.1"
                     /translation="MKKYLLIPVFTLIGVVTWAQTAEVGLIGGITYYLGDINPGIHFL
                     ASRPAYGGVARVNLNSRWTVKAEVVRGEIRGNDARGTTNDKRGLGFESKITDVGASAE
                     FNFFDYTTGSRKNILTPYIFGGVGMVFYNPEYNGIDLRSLGTEGQNAGFDGRSPYGKV
                     TFTIPFGLGIKYSINRRLGLTAEWGMRKTFSDYLDDVSTTYYLNGTQIDPGDPAEFLS
                     DPTRLHQPMQERGNPTTKDWYNFAGISLTYKFRLYNKNKCPNQWKSDYD"
     gene            2436..3191
                     /locus_tag="PKM34_01505"
     CDS             2436..3191
                     /locus_tag="PKM34_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNQ82286.1"
                     /translation="MSYKEKLDLEKLPQHIAIIMDGNGRWAKLRGKPRTFGHKHGVTA
                     VREASEAAAELGVKYLTLYAFSTENWKRPKFEVDALMHLLLQSINKETKTLNKNRIRL
                     NAIGNLEALPESIYKQLIKTIEQTAANDRMTLTLALSYSSRWEIVNATKRIVSEFAEG
                     KITLENITADYFTSMLPSFPLPEPELLVRTSGENRISNFLLWQIAYSELIFTPKLWPD
                     FRKEDLYEAIFNYQHRERRFGKTSDQINNHQIP"
     gene            3160..5871
                     /locus_tag="PKM34_01510"
     CDS             3160..5871
                     /locus_tag="PKM34_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNQ82287.1"
                     /translation="MTRSIIIKYPKVNYRKILQRNPVLIPRVKSGKLFLVLALLISGF
                     ASGVFAQVSLGDQSVIDYTRPIEYQIGGITVTGAEYLDKNVLVMLTGLNVGDRVTIPG
                     EDISGAIKKLWDQGLFEDIGLYITDIQGETVFLNLKLKERPRLSRFSLRGINKTEADN
                     LREKLNITRGDVVTESLLKNTRHRIREHFRDKGFLDAEIDIRQNPDTTRANNVVLDIF
                     IKKGNRVRIDEIRIFGNNEFSAQKIKNSLKETKERGKFNPLQDVELALLHNIREILRM
                     NPQGVVDSTMAHFNRTVKIRIFKPSRFIQDKYEEDLVKVIEKYNKVGYRDASIISDSI
                     YRNADRTLSIDLKMAEGRKYYFRNITWVGNTKYTADQLNRVLKIDRGDVYNRELLETN
                     LSFNPNEQDVSSLYLDDGYLFFSAEPVEINVENDSIDIEIRIREGKQARINKVAISGN
                     TKTSDHVVMRELRTRPGQLFSRSDIIRTTRELAQLRYFDPEKITPDIVPNPADGTVDI
                     TYKVEETSSDQIELSGGWGYGRIIGTLGLSFNNFSARKLFKSDAWKPVPSGDGQKLSL
                     RLQSYGKGYLSYSASFTEPWLGGKKPNAFSVTYWHSLYSNGKSKNDPLRSEFVIDGLT
                     LGLGRRLTWPDDFFTLYQSVTGQRYALENYSQIFVFGSGTGVYNNINYNIVLGRSSIS
                     QPIYATYGSDVSVSLEVTPPYSLFSKDDYSVMEPDERYKWIEYHKWKVNATFFTEIFP
                     KAVIMTRAKLGFLGLYNRDIGITPFERFYLGGDGLSGYNNLDGREIIGMRGYRNETIT
                     PDYYKSKNIGGTIYAKYTLELRYPLSLNPSATIYAMAFLEGGNSWLGFDDFNPFSIYR
                     SAGFGVRVFLPMFGLLGLDWGYGFDDVPGLPSANGSQFHFSINSSID"
     gene            5939..6670
                     /locus_tag="PKM34_01515"
     CDS             5939..6670
                     /locus_tag="PKM34_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNQ82288.1"
                     /translation="MKKLILISALLMMAFMPGFSQKYAFVDTDYILNNIPEYTDAQAL
                     LDDLSAEWQKEIEAKFQEIDKLYREYQAESILLPEDLKKQKENEIVQKEKDAKDLQKK
                     RFGKDGDLFKKRIELVQPIQEKIYNAIQDMAVTRNYAFIFDKASGAALLFADAKFDLS
                     DDVLDEVGTVMQTVKRENRKRQTVELGGTNTGTGTGKGSDLSPGQMVPQARPGQGTNV
                     NPTERPGSEGIKDNPMPPPGEKKPR"
     gene            6729..7244
                     /locus_tag="PKM34_01520"
     CDS             6729..7244
                     /locus_tag="PKM34_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNQ82289.1"
                     /translation="MKKILLFVMVIALSTVMSAGFGQVNAKLGYIDSNELLELMPGKD
                     SIQTALQDYQKTLETQLQTMYAEYQTKVQDYQTNSRTMSDIIRQTKEKELADLETRIQ
                     TFQQQADTDLQNKQVELLQPLLDKAKNAINAVAKENGYTYIFDVGTGAFLFYEKGDNI
                     LPLVKAKLGLK"
     gene            complement(7307..7822)
                     /locus_tag="PKM34_01525"
     CDS             complement(7307..7822)
                     /locus_tag="PKM34_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNQ82290.1"
                     /translation="MALIKTVKGIIPNIGKRCFLADTATIIGDVILGNDSSVWFNAII
                     RGDVHYIRIGHHVNVQDGVIIHCTYRQAPVTIGNNVSIAHNAIVHGCTIHDNVLIGMG
                     SILMDRVIVHSNSIIAAGALVTKGTVVEPDSVYAGAPAKKIKSIDKSLSEGEIQRIAN
                     SYNMYAGWYKL"
     gene            complement(7833..9443)
                     /gene="nadB"
                     /locus_tag="PKM34_01530"
     CDS             complement(7833..9443)
                     /gene="nadB"
                     /locus_tag="PKM34_01530"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNQ82291.1"
                     /translation="MRKTIDFLVIGSGIAGLSYALKVADKGTVCIITKARADETATKY
                     AQGGIAAVMYTPDTYAKHILDTMRAGDDLSNPEIVKIAITESTERVKELMDWGVDFDK
                     KESGRFDLHKEGGHSEFRVLHHKDQTGAEIERALLSKVKDHPKIEMLENHFTVDLITQ
                     HHLGSEVNRRTPGITCYGAYVLNPDTGEIHTLLSKITLLATGGAGQVYANTTNPVIAT
                     GDGIAMVYRAKGIIENMEFIQFHPTTLYNPDETPSFLITEALRGFGAILKTKAGTEFM
                     HKYDVRGSLAPRDIVARAIDNEMKITGDDYVCLDCRHLDEKELIHHFPTIYAKCLSLG
                     INIAKDMIPVVPGAHYTCGGIKVDEYARSSIINLYATGECASTGLHGANRLASNSLLE
                     SAVFSHRAALDSREKADHTAINTNVPDWNAEGMILNEEMVLITQSLKEVQSIMSSYVG
                     IVRSDLRLKRAFDRLRIIYRETEDLYNKSILTVKLCELRNLINIGYLIIRMALERKES
                     RGLHYSIDYPHAKAKESIDHQENGLADY"
     gene            complement(9486..>10746)
                     /gene="odhB"
                     /locus_tag="PKM34_01535"
     CDS             complement(9486..>10746)
                     /gene="odhB"
                     /locus_tag="PKM34_01535"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNQ82292.1"
                     /translation="SISEVQLAGWLVEDGEYVEKDQDIAEIDFDKATLSISAAESGMI
                     RILKAKGENVGVGEVVATIDTDGKQAPARQKKEVIPAKPVQKIEIEGVTAQDKPPVAA
                     EITEEKQEEAKLHLSPLAKKLLDANNLSQAEFIEYLKQVRFGKSDIEFFIKEKEKNKD
                     HISDSPLVIPSKPSKDSRETERRQMSTLRLKLAERLVSVKNETAMLTTFNEVNMGQIM
                     KIRQQYKESFKEKYGISIGFMSFFTKAVTEALQLFPQVNARIDGDEMVFHHFADIGIA
                     VSTPKGLMVPVLRNAENMSIADIELNIKELATKARDGKISLDEMKGGTFTITNGGVFG
                     SMLSTPIINPPQSAILGMHNIVERPIAVNGQVEIHPIMYVALSYDHRIIDGRESVSFL
                     VKVKEMLENPARMLFGGNDPVGRLLGL"
BASE COUNT         2885 a         2561 c         2482 g         2818 t
ORIGIN      
        1 ttaccagtat aaaaacgatc accgtgattt taaatcatat cccctgaaag gttattacgc
       61 tgattttggt ctcgtcaaga atggattgtg gacctttcct gaaaagacca tcaattccat
      121 ccaaataacg gccacttacc ggaaatactg ggagctaaat ccccggattt accttgcaac
      181 aggtatcaat gggtattttt ctgccggccg ccagccttat tttgtcctgg gaggaattgg
      241 ctacgaccgt gatattgtaa gagcatatga gtactatctg attgatgccg aacattttgt
      301 tattttgaaa aataatgtca agttcgccct gatcccgaac cgggagcatc gttttcaatt
      361 catcagggcc gaaaagtttg cgcgaatttt ttacgccctc taccttaatt tattcgtgga
      421 tgccggatac ggttattatt cgcaggagtt cgggcagaca acaaatgacc tgcaaaattc
      481 tatgctgatc ggatatggtg cagggctcga tttcgtcacc tattatgatg tggttgtcag
      541 gctcgatttt tccgtcaatt acaggaatga agccggattt tatcttcatt tccgggctcc
      601 gatataatct gatgaaaatg atcattattc gttacttttg cacaagccgt ttaaaatgat
      661 aagcatatgg tgattgcgct gttcggtaaa ccgatttccc aggaaaattt gaaatatgtg
      721 caaagtgtca ttgataagct attgctgaag aaatgcagag tgcttttcca caggcaattt
      781 tatgagtcgc tggcggggaa ggtggaaaca ggtgaaaata ttgggttctt cgatgatatg
      841 gaggatctgg ccgggaaggt ggattatttt atttccattg gcggtgacgg gaccatcctc
      901 gacgccgtta cactcgtcag ggattccgga atcccgatca taggcattaa tatcggccgg
      961 ctgggttttt tgtcgagtat tggtaaggat gagattttgc ctgccattga taaagtcctg
     1021 gatggggatt tcaggataga ttcccggaca cttgtcgcgc tttcgcagcc gaaggatctt
     1081 tttggcgatc tcagttatgc tttgaatgaa ctgactatca cccgtaaaga tacgacttca
     1141 ctgatcgtgc tccatgttta tatcgatgac aagtatttga actcttattg ggccgacggt
     1201 ctgatcatcg ccactccaac cggttcaacg gcctattcgc tgagcagcgg cggcccgatt
     1261 ctaactcccg gttccaggaa ttttgtcctg actccgattg ctccgcacaa tttaacggtc
     1321 aggcctgtcg ttatacctga catgtcgtca atccgggtca tcgttgaagg acgggaggac
     1381 cagttccttg tcagccttga ttccagaaag gcaactatcc attcaaattt tgaacttatg
     1441 gttgaaaagg ccgcattcag cattaatatg atccagatgc gtgagaagga cttttatatg
     1501 accatcaggg aaaagctcaa atggggcctt gacgctcgca actgaacagt tgattaatct
     1561 ttaatttttt ttgatatttt tgcggcctgc aagataagat gaagaaatac ctgttaattc
     1621 ctgtatttac gttaatcgga gtggtaacct gggcccagac agcggaagtc ggcttgatcg
     1681 gcggaatcac ctattacctg ggagatatca accccgggat ccattttctg gcgtcacgac
     1741 cagcctatgg cggggtggcg cgagtaaatc ttaattcgag gtggaccgtg aaagccgaag
     1801 tggtccgcgg ggagatccgg ggcaatgatg cccggggcac aaccaatgat aaaagaggct
     1861 tgggattcga atccaaaatt accgatgtgg gagcctcggc cgagtttaat ttcttcgatt
     1921 atacaaccgg gagcaggaag aatatcctga caccgtatat cttcggaggc gtcggaatgg
     1981 ttttttataa tccggaatat aatggtatag atttgcgcag ccttggaaca gaagggcaga
     2041 acgccggctt cgacggacgt tcgccctatg gcaaagtgac ttttaccatt ccctttgggc
     2101 tgggaataaa atatagcatc aaccgtcgac tgggtttgac tgctgaatgg ggcatgagga
     2161 aaactttttc cgattacctg gacgatgtca gcacaacata ttatctaaac ggaacacaga
     2221 tcgatccggg tgatccggcg gaatttctct ccgatccgac ccggctccat caaccaatgc
     2281 aagaaagggg taatccaaca accaaagatt ggtataactt tgcaggaatt tcactgactt
     2341 ataaattcag gctttataat aaaaataaat gcccgaatca atggaaatca gattatgatt
     2401 gatgggttgt taaagaggca agacgttaaa aaataatgag ttataaagaa aaactggatc
     2461 tggaaaagct accgcaacac attgctatca tcatggatgg aaatggacgt tgggctaaat
     2521 tgcgtggcaa acccaggacc tttggccata aacacggcgt gaccgctgtc agggaagcct
     2581 ctgaagcagc ggctgaatta ggcgttaaat atctgacgct ctatgctttc tccactgaga
     2641 actggaagcg gccaaaattt gaagtggatg ccctgatgca ccttctttta caatcgatca
     2701 acaaagaaac caaaacactg aataaaaacc ggatcagatt aaatgccata ggtaacctgg
     2761 aagccctccc tgaaagtatc tacaaacaac tcataaaaac aattgaacag actgcagcta
     2821 acgaccggat gacacttacc ctggccctga gttacagctc acgctgggag atcgtgaatg
     2881 ctaccaaacg aatagtgtcg gagtttgccg agggcaagat aacgctcgaa aatatcaccg
     2941 ccgattattt cacatccatg cttccttcat tcccacttcc tgaaccggaa ctccttgtcc
     3001 gcaccagcgg ggaaaacagg atcagcaact tcctgctatg gcaaattgct tattcggaat
     3061 tgatatttac cccgaaacta tggcccgact tcagaaaaga agacctgtac gaagccatat
     3121 ttaattacca gcaccgcgaa cgaaggttcg gtaaaaccag tgaccagatc aataatcatc
     3181 aaatacccta aagtgaacta ccggaaaatt ctgcagcgaa accctgttct tattccccgt
     3241 gttaaatcgg ggaaattatt cctggtattg gccttgctga tatccggctt tgcttccggg
     3301 gtattcgccc aggtatctct cggggatcag agcgtaatcg actatacccg gcccattgaa
     3361 taccagattg gtgggattac cgttaccggc gccgaatatc tcgataaaaa cgtgttggtc
     3421 atgctcaccg gtctcaatgt cggtgaccgt gtcaccatcc ccggggaaga catctcaggt
     3481 gctatcaaga aactatggga tcagggcctg tttgaagata tcggattgta tatcaccgat
     3541 atacaggggg aaacagtctt tctgaacctg aaactgaaag aaagaccacg gctttcccgt
     3601 ttttcactga gagggatcaa taaaacagaa gctgacaacc tgcgcgaaaa gctcaatatc
     3661 accagagggg atgtggttac ggaaagcctt ctcaaaaata cgcgccaccg gatcagggaa
     3721 catttccggg ataagggatt tttggatgcg gagatcgaca tcaggcaaaa ccccgataca
     3781 acacgtgcca ataatgttgt ccttgatata tttattaaga agggaaatcg tgtcaggatc
     3841 gatgaaatcc ggatcttcgg aaacaatgaa ttttcagccc agaagatcaa aaattcgctg
     3901 aaagagacca aggagagagg gaaatttaat ccgctgcagg atgttgaact ggcccttttg
     3961 cacaatatcc gggaaatcct caggatgaat ccgcagggag tggtcgattc gacaatggcc
     4021 cacttcaacc gcacggtgaa aatcaggatt ttcaaaccct ccaggtttat ccaggacaaa
     4081 tatgaggaag acctggtcaa ggtcattgag aagtataaca aagtcggata ccgtgacgcc
     4141 tccatcatca gcgattccat ttaccgcaat gctgaccgga ctctcagtat cgacctaaaa
     4201 atggcagagg ggaggaagta ctatttcagg aatattacct gggtcggaaa tacgaaatac
     4261 acagccgacc aactgaacag ggtactaaag attgacaggg gagacgtgta caaccgggaa
     4321 ttgctggaaa caaacctatc attcaacccg aatgaacagg atgtcagttc gctatacctg
     4381 gatgacgggt atttgttctt ctccgccgag ccggttgaga tcaatgttga gaatgattcg
     4441 atcgacatcg agatcaggat acgggaaggc aaacaggcac ggatcaacaa ggtggcaatc
     4501 agcggaaata ccaagaccag cgaccatgtc gtgatgcgcg agctgcgtac ccgcccgggc
     4561 cagctattca gccgctccga tataatccgc accacccgcg aacttgcaca acttagatat
     4621 ttcgacccgg agaaaataac cccggatatc gtccccaacc cggcagacgg aaccgtcgat
     4681 attacctata aagttgaaga aacttcttcc gaccagatcg aactcagcgg cggatgggga
     4741 tatggccgta tcataggtac ccttggcctc tctttcaata atttttccgc ccggaaatta
     4801 ttcaaaagcg atgcctggaa accagtccct tcgggcgatg gtcagaaatt aagcctccgg
     4861 ctgcagtcgt atggaaaagg gtacctgtcg tacagtgctt cttttaccga accatggctg
     4921 ggaggcaaga aaccaaacgc tttcagtgta acgtattggc attcacttta ttcaaacgga
     4981 aaatccaaga atgacccgct ccggtcggaa tttgtgatcg atggattgac gctcgggctg
     5041 ggacgaagac ttacatggcc ggatgacttc tttaccttat accaatcggt aaccggtcag
     5101 cgttatgcgt tggaaaacta ttcccagata tttgtgtttg gatcgggcac aggcgtttac
     5161 aataatatca actataacat tgtccttgga agaagttcca tcagccagcc catctacgca
     5221 acatatggtt cggatgtatc tgtatccctt gaagttacac cgccttattc gctttttagc
     5281 aaggacgact attcggtcat ggaaccggat gaaagataca agtggatcga ataccataaa
     5341 tggaaggtga atgccacatt cttcaccgag atatttccca aagcagtcat catgacccgg
     5401 gctaagcttg gattccttgg actctataac agggatatcg ggatcacgcc gtttgaaagg
     5461 ttctatctgg ggggcgatgg attgtcgggg tataataacc ttgatggcag ggagatcatc
     5521 ggaatgaggg gataccggaa tgaaaccatc actcccgact attacaagag caaaaatatc
     5581 ggcgggacta tctatgccaa atatacgctg gaactaagat acccgctatc gctcaatccc
     5641 agcgcaacga tctatgccat ggcattcctt gaaggaggta actcatggct tggatttgat
     5701 gattttaatc ctttctccat ataccgcagt gcaggatttg gggtcagggt cttcctgccg
     5761 atgttcggcc tcctggggct tgactgggga tatggatttg atgatgtacc cgggctcccg
     5821 tcagccaacg gctcccagtt ccacttctcg atcaactcgt caattgatta accgaagcta
     5881 atatggcagg ttttttgctg gtaatccttt tatcattaaa tatattggag gaataaatat
     5941 gaaaaagctc attttaatct cagcgcttct gatgatggcc tttatgccag gttttagtca
     6001 gaaatatgca ttcgtcgata ccgattatat cctgaataac atacctgagt acaccgatgc
     6061 ccaggctttg ctggatgacc tctcagccga atggcagaag gaaatcgaag cgaaattcca
     6121 ggagatcgat aagctttaca gggaatacca ggcagaatcg atcctgttgc ccgaagatct
     6181 gaaaaagcag aaagagaacg agatcgttca gaaggaaaaa gatgccaaag atcttcagaa
     6241 aaaacgcttc ggtaaagacg gtgacctctt caaaaaaagg atagaactgg ttcagcccat
     6301 ccaggagaaa atctacaacg caatccagga tatggccgtg acacggaatt atgcctttat
     6361 tttcgacaaa gcatccggag cggcattgtt gtttgccgat gctaagttcg atctcagcga
     6421 cgacgtcctg gatgaagtcg gtaccgtgat gcagaccgtt aaaagggaga accgtaagcg
     6481 ccagaccgtt gaactgggag gcaccaatac gggtaccgga accggaaaag gctccgatct
     6541 ttcacccggg cagatggtac cccaggcacg gcccggacag ggaacaaatg tcaatccgac
     6601 agaaaggcct ggttctgagg gtataaaaga taaccccatg ccgccgccgg gtgaaaaaaa
     6661 accaaggtaa taggaaattc ataaatttcc atatctttgc cgcacttaat taattaatca
     6721 tactgaaaat gaaaaagatc ttattatttg tcatggtgat tgccctcagc actgttatgt
     6781 ccgccggatt tggacaggtg aacgccaaac tgggctatat tgattcaaat gaattattgg
     6841 agttaatgcc gggaaaagac tccattcaaa cggcactgca ggattaccag aaaacgctgg
     6901 aaacccagtt acaaaccatg tatgccgagt accagacaaa agttcaggac tatcagacaa
     6961 attcccgcac catgtcagat ataattcgcc agacaaaaga aaaagaactg gcagatcttg
     7021 aaacccgtat tcagactttc cagcaacaag ccgataccga tttgcagaac aaacaggtgg
     7081 aacttctgca accgctgctg gataaagcaa aaaatgccat taacgccgtc gctaaagaga
     7141 atggatatac ctatattttt gatgtcggta ccggcgcttt ccttttttat gaaaaaggag
     7201 acaatatcct gccgctcgtc aaagccaaac tgggattgaa ataaatttcc actgatcaaa
     7261 taaaagagcc tgactcaaaa tcctgagcag gctcttttat atttttttac aacttgtacc
     7321 atccggcgta catgttatag ctgttggcaa tccgttgtat ttccccttcc gataaacttt
     7381 tgtcgatgga tttgattttt ttggcagggg ctcctgcata aacgctgtcg ggttccacga
     7441 ccgtcccttt cgttacgagc gctcctgccg caattatgct gttggaatga acaatgaccc
     7501 ggtccataag gatggatccc atgccgatca ggacattatc atggattgta cagccatgta
     7561 ctatcgcatt gtgtgcaata gatacgttat tccctatggt gacgggcgcc tggcggtagg
     7621 tacagtgtat gatcacacca tcctggacat tcacatggtg cccgatccgg atgtaatgaa
     7681 catcgccccg tatgatcgca ttgaaccaca cactgctatc atttcccagg ataacatccc
     7741 cgataatggt tgccgtatcg gccaggaaac accgtttccc gatattcgga atgattcctt
     7801 ttactgtttt tatcagtgcc atgacagaac ggttaataat cggcaaggcc attttcctgg
     7861 tggtctatcg actctttcgc tttggcatgc ggataatcga tcgaatagtg caaaccacgg
     7921 ctctctttcc tctccagtgc cattctgata ataagataac cgatattgat caggttgcgg
     7981 agttcgcaca gtttcaccgt taaaatggat ttattgtaga ggtcttcggt ttcacggtaa
     8041 atgatgcgca accggtcgaa tgccctcttt aaacgcaggt cggatctgac gatcccgaca
     8101 tagctcgaca tgatggactg cacttctttt aagctttgtg tgatgagcac catctcttcg
     8161 ttcagtatca tcccttctgc attccagtca ggaacatttg tattgattgc tgtatgatcc
     8221 gctttttccc ttgaatcgag cgctgcccgg tgcgaaaaga cagccgattc gaggagggag
     8281 ttggaagcga ggcggttggc cccgtgcaaa cccgtcgatg cacattcgcc ggtagcatag
     8341 aggtttatga tactgctgcg cgcgtattca tccactttga tgccgccgca ggtataatgt
     8401 gcgcccggca ctacgggaat catatctttg gcgatattga tgcctaaact gaggcatttg
     8461 gcataaatgg tcggaaaatg atggatcagc tccttttcat ccaggtgacg gcaatccagg
     8521 caaacataat catcccctgt gatcttcatt tcgttgtcga tcgcccgggc cacgatatcc
     8581 cgcggtgcca gcgaacccct cacgtcatac ttatgcatga attcggttcc tgccttggtc
     8641 ttcagaatgg cgccaaagcc ccggagcgcc tccgtgatca gaaacgaagg tgtttcatcc
     8701 ggattatata atgtggtagg atgaaactga ataaactcca tattttcaat gatccccttc
     8761 gcccggtaca ccatggcaat accatcgccg gttgcaataa ccgggttggt ggtattggca
     8821 tacacctgtc cggctccgcc cgtcgccagc aaggttatct tcgacaacaa ggtgtgtatc
     8881 tcacctgtat ccggattcag cacataagcg ccgtagcacg taatgcccgg tgtcctccgg
     8941 tttacctcag aacccaggtg gtgctgggta atcaggtcga ccgtgaaatg attctcgagc
     9001 atttcaattt tagggtgatc cttgacctta ctgagcaacg ccctttcgat ctcagcccct
     9061 gtctggtctt tatggtgcag caccctgaat tccgaatgcc caccctcttt gtgaagatcg
     9121 aaccttcccg attctttttt atcgaaatcc acaccccagt ccattaactc ttttacccgt
     9181 tccgtcgatt cggtgatggc aattttgacg atctccggat tgctcaggtc atcgcccgcc
     9241 ctcatcgtat caaggatgtg tttggcatag gtgtcgggcg tgtacatcac cgctgcgatt
     9301 ccgccctggg catatttggt ggctgtctca tcggccctgg ctttggtaat aatacatacc
     9361 gttcctttat ccgccacttt gagagcgtag ctcaaacccg caatgccgga cccaatcaca
     9421 agaaaatcta tcgtcttacg catacctcaa tttttccact gttccactgt tccaccttac
     9481 cacccttaca accccaacaa cctacccaca gggtcatttc ccccgaaaag catcctggcc
     9541 ggattttcaa gcatctcttt cactttaacg aggaaactca ccgattccct gccgtcgatg
     9601 atccggtgat catacgatag cgctacatac atgatggggt gtatctcaac ctgcccgttc
     9661 acggcaatag gacgctccac gatattatgc atccccagga tggcactttg cggcggattg
     9721 atgatcggcg tcgaaagcat ggaaccgaat acacccccgt tagttatggt aaatgtcccg
     9781 cctttcattt cgtcgaggct gatcttgccg tctcttgctt tagtagccag ctctttgatg
     9841 ttcagctcta tgtcggcaat agacatattt tccgcatttc ttaaaaccgg aaccattaac
     9901 ccttttggcg tgctgactgc aattccgatg tcagcaaaat gatggaatac catctcatcg
     9961 ccatctatcc gtgcattgac ctggggaaaa agctgtaacg cttccgtgac cgcttttgtg
    10021 aaaaatgaca tgaacccgat agaaatgccg tacttttcct taaaggactc cttgtattgc
    10081 tgcctgatct tcatgatctg acccatgttc acttcattaa atgtggtcag catggccgtt
    10141 tcatttttaa ccgaaacaag ccgttcggcc agctttaacc ggagggtgct catttgcctg
    10201 cgttccgtct ccctggaatc tttggagggt tttgacggga taaccaatgg actatcagaa
    10261 atatgatctt tatttttttc tttttctttt ataaagaatt cgatatccga tttgccgaaa
    10321 cgaacctgtt tcaggtattc aataaactcc gcctggctaa gattattggc gtccagaagc
    10381 tttttggcca acggcgacaa gtggagcttt gcttcttcct gtttttcttc cgttatttca
    10441 gcagcaacgg gaggcttatc ctgtgctgtc accccttcga tctcaatctt ttgaactggt
    10501 ttcgcaggaa taacctcctt tttttgcctg gccggggcct gtttgccgtc tgtgtcgatg
    10561 gtggctacga cctctccgac gccaacattt tcgcctttgg ctttcaggat gcggatcata
    10621 cccgactccg ccgcgctgat actcagtgtc gctttatcga aatcgatttc ggctatatcc
    10681 tggtcttttt caacatattc accatcttca accagccagc ctgccagctg gacttcgctg
    10741 atcgat
//
LOCUS       DAOQXS010000026        10664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6658_length_10664_cov_3.708267, whole genome shotgun sequence.
ACCESSION   DAOQXS010000026 DAOQXS010000000
VERSION     DAOQXS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6658_length_10664_cov_3.708267"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKM34_01540"
     CDS             <1..428
                     /locus_tag="PKM34_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82293.1"
                     /translation="ASFIGIYDKDVSEMYFPYVRPQENGTRTDIRWMTLTNPSGDGIM
                     IAGMPLVSVSALPFTTSQLDYTDNMFRHTTDLIPNYFIDINVDYMQTGVGGNDSWGAR
                     PLSKYTLFPSDYRFSFRIRPINNKIDPAKAGKVVFSPLK"
     gene            436..2811
                     /locus_tag="PKM34_01545"
     CDS             436..2811
                     /locus_tag="PKM34_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HNQ82294.1"
                     /translation="MKKILIPLIIISVSIFGCTGDKKEKATRPDIIPIPMNYSMGEGN
                     FTLNRKTDIFISLADNYLKDVAVLLQERIALLTGSEPEINILTGEQPKNSILLRLDGS
                     ISIEAGLEGYKLSVERSRIVISAPQPAGIFYGMQTIFQLLPPSAFTAGNSKAGLEDVK
                     WTVPCVEITDKPAFAWRGMHLDVSRHFFPREFILKYIDMIAMHKMNVFHWHLTDDNGW
                     RIQIDKYPLLTEVSAWRADRSDIPWNERAPQRPGEKATYGGFYTKEDIREIVEYARRR
                     FITVVPEIEMPGHTSEVFAAYPQFSCTGERTTVQTGSYWPNEDIFCAGKEETFRFIED
                     VLDEVIAMFPSPYIHIGGDEADKTAWKKCTDCQSRIRKEGLSGVDELQSYFVKRIERY
                     IKSKGRILIGWDEILEGGLAPDATVMSWRGFEGGIDAARQGHKVVMCPTSYCYFDYYQ
                     ANPDFQPPAIGGLITLKKVYSFRPKPSELSPEEQKLILGAQGNLWTEYVPEPDHAEYM
                     VLPRMTALAEVLWSPEPLLVWDDFKERLLTQNKRFGAMEANYFKGSGRVDATAVFNKD
                     DKPYAIKLETEKDGTKIYYTLDGYTPDKRSFMYKNPVAITNNVTLKAVAYKGDKQLEL
                     PVEYSIKFHKGVGKNVSYNLNYSERYTGGGGYALVDGLRGSLNYNDGFWQGWNGRNAD
                     FVISLGDEFYFKNISATFLLDQKRWIFYPEVVNFYVSDDGKNFQKIGSIRHLIDMNDP
                     AAQTNDFTVKISQPLKVKFLRIEAVNIGVCPDWHPYHGEKSWIFLDEVVLN"
     gene            2835..4784
                     /locus_tag="PKM34_01550"
     CDS             2835..4784
                     /locus_tag="PKM34_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82295.1"
                     /translation="MKKLFLIVLLLSGSLSGPAQADTSGYMAMPRLNYYTNEEVGEII
                     VITPGQLIGKKVTVDLVFEYEFLNRGFVVVPGALTTVPFEMKRLREGENEITVSFYEN
                     DKWTDSRKTYLTIRPHKENAVKVDQATGGLFVSGLPYFPAGFFAGFPVNPDLPGQEAV
                     NGFNMISPYQDLNKKTFKQRKAYMDRCANLGIRVNYNVSSGLYGGRDENCDQAGNEKP
                     VDKERLMKEILAMRDHPALLSWYIAEEPDGRDIPADSLAVYYHLIRELDPYHPVSMMV
                     STPRKADSYLGVTDIILINPNPIPYGTIREVYDYTRIAVGKSWPGKPVWIVPQAFGGN
                     RMWSREPNAAEVRAMTYMAIIHGATGVQFTLREPPNSFPKSTATWGECSRIAMELAEL
                     TSDLLSPHPAPRVLASEAGIHARAWNRRGMVTIAIVNEKNEPLNFSLEMEDFNVSVMA
                     DLLFENRKVAIVEGIIKDVIDGYGTRIYRFDARQRTGQEILPDPQNLTTDPGFEDMTT
                     AGVPSACYAYPGADPGCTYFTDPRRHYEGDHSLRMNNPSAGPGNLLSFYPVNVDSAMT
                     YTVSIMARSGPSPNKSGGKKGGPVRFRLAFGPAEQVFECTTAWEKYEIKGIKLDGASP
                     AQVSPELELIGKGTAWFDLIQVVAE"
     gene            complement(4797..5294)
                     /locus_tag="PKM34_01555"
     CDS             complement(4797..5294)
                     /locus_tag="PKM34_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ82296.1"
                     /translation="MIFHSLASEAVMRALARRLGRDEEQWGQAGLLHDLDVEITDADP
                     YVHGLETARILTEMGVDADLVEAIKLHNEMAASQPRTAEFHHALAAGETITGLIVATS
                     LVYPDKKIASVKPKSVVKRMKEKAFAASVRRENIMECELIGIPLDEFAEIALTAMTAL
                     ETANN"
     gene            complement(5758..7029)
                     /locus_tag="PKM34_01560"
     CDS             complement(5758..7029)
                     /locus_tag="PKM34_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ82297.1"
                     /translation="MELLIILLLIVLNGVLSMSEIALVSAKKSRLQYMARKGNKQAAR
                     ALDLSNRPDRFLSAIQIGITLVGIFLGAYSGENITEDMQGILNRVTALQPYSHTLSFI
                     LVVLIITFFSLVLGELVPKQLGLAMPEKLAKAMAGPVHVFSVVTRPFVWLLSISTNLI
                     IKAFNIKASDDEKITEEEIKSMIEEGTRHGEVQDVERDIVDRVFILGDTTIKSLMTYK
                     SDIVWIDFSDEQEEIRQKVSANLHNVYPVAEGSLDQVMGTVGLKDLFTNLDKPGFSLA
                     NCIHDPFYLPENTSAYDAIRQFRLNKTFHALVIDEYGSVEGIITVSDILESLVGEVSP
                     ADEEYQIISRDDGTWLVDGQYPFHEFLNYFDLQELYEENRFNTIAGLILDQLGYIPKA
                     GDKTDWMGFRFEIMDMDRARIDKVLVSKEKP"
     gene            7171..8490
                     /locus_tag="PKM34_01565"
     CDS             7171..8490
                     /locus_tag="PKM34_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNQ82298.1"
                     /translation="MSTRAYIISITIIGVFFFIFGFVTWLNGILIPYLKIACEISDFQ
                     SLFVAFAFYISYTVMALPSSWMLKKTGFKNGMSLGLAVMAAGSILFIPAAISRSFPLF
                     LTGLFVMGTGLAILQTASNPYITIIGPRETAARRISIMGICNKLAGAIAPLILAYFIF
                     SDGDAFIKSLEGMDNAAKAAALDNLAGRVIEPYAVMAAILLLLGLLIRFAPLPKVETE
                     EESPEELSAIAGKTNILQFPHLVLGAIALFFYVGIEVVAGDTIIRYGMSLGVPIESAK
                     YYTSLVLIAMVIGYIIGIILIPKILSQKNLLAVSAVTGIVFSALAIFVPASATMQFPF
                     FDLVTLQSIDIRLPYTVLFVALLGISNAIVWPAIWPLAIHGLGRFIKTGSAILIMSIA
                     GGALLPLLWGYLSDVWSAQQAYWIGIPCYLFILYFAVSGHKVGRKEV"
     gene            8523..9434
                     /locus_tag="PKM34_01570"
     CDS             8523..9434
                     /locus_tag="PKM34_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ82299.1"
                     /translation="MEPTLLILAAGIGSRFGGLKQASGAGPSGEAILDYSIYDAVRAG
                     FKKVVFVIRRDIYDDMKKIFFDRWTHKIEIDYIFQELNDLPGGYTAPPDRVKPWGTGH
                     AIWVAREKISEPFCMINADDFYGRKSFELAAGFLRNMDNLIHSRYALIGYILKNTLSE
                     HGFVSRAECETDDTGKLTAITERLKIMREGESVIFSDESGAVMNISENAVVSMNMWGF
                     MPSLFRYLEEDLIRFLQENSTSAKAEFLLPNVIDRLIKSGEVDIPVMQTDSKWFGITY
                     KEDLEMVKGKLQKLVESGSYPSPIWIF"
     gene            9450..10499
                     /locus_tag="PKM34_01575"
     CDS             9450..10499
                     /locus_tag="PKM34_01575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNQ82300.1"
                     /translation="MSNPLYNIAGLFNISSPAISIVRYGEGHIHDTYLVTTGSSQPDY
                     ILQKINHKIFRDIPGMMRNIKEVTDYLRFKNTGKSSTFSTPELVYTYEGQPFLELDEE
                     NFWRMYVYIPDTVTYQKMEDPALGFEAGKAIGRFQAGLAGLPVPLVETIPGFHDIFLR
                     IEQYYSARENDPLSRKGKIMDTIDFAEKRFDTAKNYLLQLREKGVIRATHNDTKLNNI
                     LFSRQNEALCLIDLDTVMPGYVHFDFGDALRTMANTALEDEPDLKRVRFNEKVYQSVT
                     KGFLAEAGPMLNEAEKELLPYAPVYMTFIIGLRFLADYLNGDTYFRIHHPDHNLDRAR
                     VQFKLVQEIEQFLKA"
     gene            complement(10459..>10664)
                     /locus_tag="PKM34_01580"
     CDS             complement(10459..>10664)
                     /locus_tag="PKM34_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82301.1"
                     /translation="MRIIRQNLFWAFFYNIIGIPVAAGVLFPFTGLLLDPMIAGAAMA
                     FSSVTVVSNSLRLSKIALSPEPV"
BASE COUNT         2795 a         2446 c         2644 g         2779 t
ORIGIN      
        1 ccgcttcttt tattgggatt tatgacaaag atgttagcga gatgtatttc ccttatgtcc
       61 gcccgcagga gaatggcacc cgtaccgata tccgctggat gacactgaca aatccttccg
      121 gcgacgggat catgatagcc gggatgccgc tggtatcggt ctctgcattg ccattcacaa
      181 ccagtcagct tgattatact gacaatatgt tccgccatac gaccgatttg attcccaact
      241 actttatcga tattaatgtc gattacatgc agacgggagt aggagggaat gatagctggg
      301 gcgcacggcc attatcgaaa tacacccttt tcccttccga ttaccggttc agctttcgca
      361 tccgtccgat caataataaa atagacccgg ctaaagcggg taaagttgta ttttcgccac
      421 tgaaatgaaa agaatatgaa aaagatactg atacctctga ttataatttc agtttctatt
      481 tttggatgta cgggtgataa aaaggaaaaa gcaacccggc ccgatattat cccgataccc
      541 atgaactaca gcatgggtga aggtaacttt acccttaacc ggaaaaccga catttttatc
      601 agcctggcag ataattactt aaaggatgtg gcagttctgt tgcaggaaag gatagcttta
      661 cttaccggat cggaacccga gattaatatc ctgacgggtg agcagcccaa aaactcaatc
      721 ctgctgcgcc tggatggctc tatttcaatt gaagccggcc tggaaggata taaattatcg
      781 gtcgagcgtt caaggatagt gatcagtgca ccgcaacccg ccggtatctt ttacgggatg
      841 cagacgatct ttcagctttt acccccttcg gccttcacag ccggtaatag caaagcggga
      901 ctggaggatg tgaaatggac cgttccttgt gttgagatta cagacaaacc cgcttttgcc
      961 tggagaggta tgcacctgga tgtctcccgc catttctttc ccagggaatt catcttaaaa
     1021 tatatcgata tgatcgccat gcacaagatg aatgtctttc actggcatct taccgatgat
     1081 aatggctggc gtattcaaat cgataaatac ccgctgctga cggaggtctc ggcctggaga
     1141 gccgatcgca gcgacattcc ctggaacgaa agggcgccgc agcgaccggg cgagaaagca
     1201 acctatggcg gtttttatac caaggaagat atccgggaga tcgttgaata tgccaggcgg
     1261 cggttcataa cggtcgtacc tgagattgaa atgccgggcc acacctcgga agtgttcgct
     1321 gcttatccgc aattttcatg cacaggcgag agaacaaccg tacaaaccgg atcctactgg
     1381 ccgaatgagg atattttttg tgcaggtaag gaggaaactt tccggtttat tgaagatgtc
     1441 ctggatgagg tcatcgcaat gttcccatcg ccttatatac atatcggagg cgatgaagcc
     1501 gataaaacgg cctggaagaa atgcaccgac tgtcagtcga ggatcaggaa ggaaggactc
     1561 tcaggcgtgg atgaactgca aagctatttt gtgaaaagga tcgagcgtta cataaaatcc
     1621 aaaggacgaa tcctgatcgg ctgggacgag attttggagg gtggtcttgc tcctgatgcc
     1681 accgtgatgt cgtggagagg ctttgaagga gggatcgatg ctgccaggca ggggcataaa
     1741 gtggtcatgt gcccgacatc ttattgctat ttcgattatt accaggcaaa tcccgatttt
     1801 caaccccctg ctatcggtgg gttgattacc ctgaaaaagg tctattcatt caggccaaag
     1861 ccttcagaac tttctccgga ggaacaaaaa ctaattctcg gggcccaggg aaatctttgg
     1921 acagaatatg tccctgaacc ggatcatgct gaatatatgg ttcttcccag aatgacggcc
     1981 ctcgcggaag tcctgtggag ccccgagcct ttactggttt gggatgattt caaggaacgc
     2041 ctgctgaccc agaataagcg ctttggtgcg atggaagcga attattttaa agggtcggga
     2101 agagtagacg ctactgcagt ttttaacaag gatgataaac cctatgccat caaacttgaa
     2161 acggaaaagg atggcactaa aatttattat acgctggatg ggtacacacc cgataaaagg
     2221 tcgttcatgt ataaaaatcc cgttgccatt acgaataatg ttactttgaa agccgttgcc
     2281 tataaagggg ataaacagct ggaactgccg gtggaatata gtattaaatt ccataaagga
     2341 gtgggcaaaa acgtttcgta taatttgaat tacagcgaaa gatataccgg tggcggcggt
     2401 tatgccctcg tcgatggcct taggggatcg cttaactata acgatggatt ctggcagggt
     2461 tggaatggga gaaatgccga ttttgtcatt tccctgggcg atgaatttta ttttaaaaat
     2521 atttcggcaa cctttcttct ggatcaaaaa cgatggatct tttacccgga agtggtcaat
     2581 ttttatgtgt cggatgatgg taagaatttt cagaagattg gatctatccg tcatttgatc
     2641 gatatgaacg acccggctgc ccagaccaat gactttacgg taaagatcag ccaacccctg
     2701 aaagttaaat ttctcaggat agaagcggtg aatattggag tatgcccgga ctggcatccc
     2761 tatcatggcg agaagtcctg gatatttctt gatgaagtgg ttttgaatta gttcatgaat
     2821 ttgattattg cagaatgaaa aaactattct tgattgttct cctgctgtcg ggaagccttt
     2881 cgggaccggc acaggctgat acctcaggct atatggctat gcccaggctg aattattata
     2941 caaacgaaga ggttggagag attatagtta ttactcccgg acaattgatc gggaaaaaag
     3001 taactgttga ccttgtcttt gaatatgaat tcctgaacag gggatttgta gtggttcccg
     3061 gagccttaac caccgtaccc ttcgagatga agaggttaag ggaaggcgaa aatgagatta
     3121 cggtatcttt ctacgaaaat gataaatgga ccgattccag gaagacgtat ctgaccatca
     3181 ggccccacaa ggaaaatgcg gtcaaggtgg atcaggcaac aggtgggctg tttgtttccg
     3241 ggctccctta cttcccggct ggtttctttg ccggctttcc tgttaatccg gacttacccg
     3301 ggcaggaggc tgtgaatggt ttcaatatga tttcacctta ccaggatttg aacaaaaaga
     3361 catttaagca acggaaagct tatatggacc gttgcgctaa tctcggaatt agggtcaact
     3421 ataacgttag cagtggttta tatgggggaa gagatgaaaa ctgcgatcag gcgggaaatg
     3481 agaagccagt tgataaggaa cggttgatga aagagattct ggccatgcgc gaccatccgg
     3541 cgcttttgag ctggtatatt gccgaagaac ctgacggtcg ggatattccg gctgattcgc
     3601 tggcggtgta ttaccatctc atcagggaac ttgatcccta tcacccggtt agcatgatgg
     3661 tttctactcc acgaaaggcc gattcctacc tgggcgtcac cgatattatc ctgatcaacc
     3721 cgaatcccat tccctacggg actatccggg aggtttacga ttatacgagg attgcggtcg
     3781 gtaaatcctg gcccggcaaa ccggtttgga ttgtaccgca ggccttcggt ggcaacagga
     3841 tgtggagccg cgaacctaat gcagcggaag tccgggctat gacctatatg gctattatcc
     3901 acggagctac gggtgttcag tttactttga gggaaccgcc aaacagcttt ccaaaatcga
     3961 cggcaacatg gggtgaatgt agcaggatag ccatggaact ggctgaattg acttccgatc
     4021 tgctttcacc acatccggcg ccgagggttc tggccagtga ggcaggtatt cacgcccggg
     4081 catggaacag gcgcggaatg gtcaccattg ccatagttaa tgaaaagaat gagccgttga
     4141 atttttcgct ggagatggaa gatttcaatg tttcggtgat ggcggatctt ttgtttgaga
     4201 accggaaagt tgccatcgtg gaaggaatta ttaaagatgt gattgatggc tatggtaccc
     4261 ggatttaccg ttttgatgcc aggcagagaa ccgggcagga gatcctgcct gatccgcaga
     4321 atctcacgac cgatccgggt tttgaggaca tgaccacagc cggagtacct tcggcctgtt
     4381 atgcctatcc cggggccgat cccggatgca cttatttcac ggatccccgg cgccattacg
     4441 aaggcgatca ttccctgagg atgaataatc cttctgccgg gcccggaaat ctgctgagtt
     4501 tttacccggt caatgtcgat tcggccatga cctatacggt cagcattatg gcccggagcg
     4561 gaccctcgcc gaataagtcc ggtgggaaga aaggcggccc ggtaaggttc aggcttgctt
     4621 tcgggcctgc agagcaggtt ttcgagtgca caacagcctg ggagaaatat gagattaagg
     4681 gaattaagct ggatggggcc agcccggcac aggtttctcc cgaattggag ttgattggca
     4741 aaggaacagc ctggttcgac ctcatacagg tcgttgccga ataagcaggt ttccgctcaa
     4801 ttattggctg tttcaagtgc agtcatggca gttaatgcaa tctccgcaaa ctcatccagc
     4861 ggaatcccta tcagctcaca ttccataata ttctcccgcc tgacagatgc ggcaaaagcc
     4921 ttttccttca ttcttttaac aacgctcttc ggtttgacgc tggcgatttt tttatcagga
     4981 tagaccaggg aagtagccac gatcaggccg gtgatcgttt cgccggcagc cagggcatga
     5041 tggaactccg cagtgcgtgg ctggcttgca gccatttcgt tgtgtaactt tatcgcctca
     5101 accaaatcgg catcaacgcc catttcggta agtatccggg ctgtttcaag cccatggaca
     5161 tacggatcgg catcggtgat ttcaacgtcg aggtcgtgca gcagtcctgc ctgcccccat
     5221 tgttcctcat ctcttccgag cctgcgggct aatgcacgca taacagcttc agaggcaaga
     5281 gaatgaaaga tcatccgttc gtttttaatg tgctgatgca gaaggcctat tgcttgttcg
     5341 cggggtaaaa gcatggtgtg tttttatgca aatttagtca atttggaaat gagacaattt
     5401 gaaaatgaga caatttgaaa atgagacaat ttggaattca ttggacaatg gacaatggaa
     5461 aatattttac cccggagggg ttccacattt gtagtgatta ggaatgccca tagcaatctc
     5521 ccctcccttg ttgttcccgg atcaaggtgt tcgggtctgc acgggagggg acgggggtgg
     5581 ggaaaggaaa tcatttcgca ttgtccattg tccatttcgc attgtccata gaagaatgat
     5641 agtcatcaga caatgcaaaa tgaaaaacag aacatgccat gcaaaatgaa aaatgcaaaa
     5701 cgcaaaatgc aaaaccacct ggaaaatggc caatggaaaa tgcgcaatgg aaaatgctta
     5761 tggcttttct ttgctcacca gcaccttatc gatccgcgcc cggtccatat ccatgatctc
     5821 aaaccggaat cccatccaat cggtcttgtc gccggctttg ggaatgtatc ccagctggtc
     5881 gaggatcagc ccggcaatgg tgttgaaccg gttctcttcg taaagctcct gcaaatcgaa
     5941 ataattcagg aactcatgga aaggatactg cccgtcgaca agccaggtgc cgtcgtcgcg
     6001 gctgatgatc tggtattctt catccgccgg ggatacttct cctaccagcg attccaggat
     6061 gtcgctcacc gttatgatcc cttccacact tccgtattcg tcgatcacca aagcatgaaa
     6121 tgtcttgttc aacctgaatt gccggatagc atcatacgct gatgtattct ccggaagata
     6181 aaagggatca tggatacagt ttgccagcga aaagccgggt ttgtcaaggt tggtgaaaag
     6241 gtctttaagg ccaacggttc ccatcacctg gtcgaggctt ccttcagcaa ccgggtaaac
     6301 attatgcaga ttggctgaaa ctttctgcct gatctcttcc tgttcatctg aaaaatcaat
     6361 ccagacaatg tcacttttgt aagtcattaa cgatttgatg gttgtatcgc cgagaatgaa
     6421 aacccggtcg acgatatccc tttccacatc ctgcacctct ccgtgccttg tcccttcttc
     6481 tatcatcgat ttgatctctt cttccgttat cttctcatca tctgaagctt ttatgttaaa
     6541 tgctttgatg atcagattcg tggagatgct taacagccag acaaacggcc tggtgactac
     6601 tgaaaaaaca tggaccggac ctgccattgc ttttgccagt ttttcaggca tagccaggcc
     6661 caactgctta ggaaccagtt cgcccagcac cagggagaaa aaggtgatga tcagcacaac
     6721 caggataaac gacagggtgt gcgaataggg ctgaagcgca gtgacccggt taagtattcc
     6781 ctgcatgtct tctgtaatat tctcgccgct gtatgctccc agaaatatcc ctaccagcgt
     6841 gatcccgatc tgtatggccg acagaaaccg gtcaggacgg ttgctcaggt ccaacgccct
     6901 ggctgcctgt ttattccctt tgcgtgccat gtattgcagc cttgatttct tcgctgaaac
     6961 aagagcgatt tccgacatgg aaagcacgcc attcaacact atgagcagta aaataatcag
     7021 gagttccatt ctggaattag aattgaacgg caaagttacg gaaagaaatc tttataaatt
     7081 tgcatccagg caatgaataa tgatttctta aattattcac atcaagaatt ttaaattaag
     7141 aattaagaat ttaacattaa acattatccc atgtccaccc gggcttacat tatttctatc
     7201 acgataatcg gcgtcttttt ttttattttc gggtttgtca cctggctcaa cgggatcctg
     7261 atcccgtatc tcaaaatcgc ctgtgaaata tctgattttc agtcgctctt tgtggctttt
     7321 gctttctaca tctcttacac cgtaatggcc ttaccctcgt catggatgtt gaaaaagaca
     7381 ggctttaaaa atggaatgag tttaggcctt gcggtcatgg cagcagggag cattttattc
     7441 ataccagcgg ccatatcgcg ttctttccca ttgtttctta cggggctttt cgtcatggga
     7501 acgggcctgg ctatcctgca aacggcttcc aatccgtaca ttacgatcat cgggccccgg
     7561 gaaacggccg cacgccgcat cagtatcatg gggatctgta acaagctcgc aggggccata
     7621 gccccgctca tcctggctta ttttatattt agcgatggcg atgctttcat taaaagcctt
     7681 gaaggaatgg ataacgcagc aaaggctgca gcgctggata acctggcagg aagagtcatc
     7741 gagccgtatg ccgtaatggc ggctattctt cttctgctcg ggttattgat ccgttttgcc
     7801 cctttgccaa aggtcgaaac cgaagaagaa agcccggaag aactttcggc tatagccggc
     7861 aaaaccaata tcttacaatt tcctcacctg gtactcggag ctatcgccct cttcttttat
     7921 gttggaatcg aagtcgttgc gggcgatacg attatacgtt acggcatgtc gctcggagta
     7981 cccatcgaat cggccaaata ttacacctcc ctggtgctca tcgctatggt tatcggttat
     8041 atcatcggga taatcctgat cccgaagatt ctttcacaaa aaaacctgct ggctgtcagt
     8101 gcagttaccg gcatcgtctt ctcggccctg gccatctttg tccctgcatc cgccaccatg
     8161 cagttcccgt ttttcgacct ggtaaccctg caaagtatcg acatccggct gccttatacg
     8221 gtattgttcg tggcgctgct gggtatctcc aatgcgatcg tatggcctgc catctggccc
     8281 ctggccattc acgggctcgg acggtttatc aagaccggct cggctatcct gatcatgagc
     8341 attgccggcg gggcgctcct gcctctgctc tggggttacc tttcggatgt ttggtcggcc
     8401 cagcaagcct attggatcgg gattccatgc tatctgttca tcctgtattt cgccgtttca
     8461 gggcataagg tgggcaggaa agaggtgtaa ggggtggaat attgaatttg gaatttttat
     8521 atatggaacc aaccttatta atattagcag ccggtatcgg aagccggttt ggcggactta
     8581 aacaggcatc cggcgcaggg ccgtcaggag aagcgattct cgactattca atatatgatg
     8641 ccgtgagagc cggatttaaa aaagtagtct ttgtaatccg ccgtgatatc tacgatgata
     8701 tgaaaaagat ctttttcgat cggtggactc acaaaattga aatagattat attttccagg
     8761 aactgaatga tctgccgggt ggttacacgg ccccgcctga cagggtaaag ccctggggaa
     8821 caggacacgc catctgggtg gccagggaaa agatcagtga acctttctgc atgatcaatg
     8881 ccgatgattt ttatggaagg aaatctttcg aactcgccgc cggttttctc aggaatatgg
     8941 ataacctcat ccacagccgt tatgctctca tcggttatat cctgaaaaat accctctcgg
     9001 aacacggttt tgtttcccgt gccgaatgtg aaacagatga cacaggaaag ctcaccgcga
     9061 taacggaacg gcttaaaata atgagggaag gagaatcggt cattttcagc gatgaatcgg
     9121 gtgcagtaat gaatatcagt gagaacgctg tcgtttcgat gaatatgtgg ggatttatgc
     9181 cttcgctttt ccgttacctg gaagaagacc tcatccggtt ccttcaggaa aattcaacca
     9241 gcgccaaagc tgaatttctg cttcccaatg tcatcgacag gctaatcaaa tcgggtgaag
     9301 tggacattcc tgtcatgcaa accgattcga aatggttcgg gatcacatac aaagaggatc
     9361 tcgaaatggt taaaggtaag ttgcaaaagc tggtagaaag cggaagctat ccttcgccaa
     9421 tctggatatt ctgaggagcc gcgatacgga tgagcaaccc tttatacaat attgcaggtc
     9481 tattcaatat ttcttcccca gccatatcga tagtccgata cggagaaggg catatccatg
     9541 atacttacct ggtcaccact ggatccagcc agcccgatta tattcttcaa aagatcaatc
     9601 ataagatctt cagggatatt cccgggatga tgcggaatat caaagaagtc acggattatc
     9661 tccggttcaa aaacaccgga aaaagcagta ctttttcaac acccgaactg gtttatactt
     9721 atgaaggtca gccgttcctg gagctggatg aggaaaattt ctggaggatg tatgtttata
     9781 tcccggatac ggtaacttat cagaaaatgg aggatcccgc tttaggattt gaggccggta
     9841 aagccatcgg acgtttccag gcaggtctcg ccggtttgcc ggtaccattg gtggaaacca
     9901 tccccggttt tcatgatatc ttcctccgga tagagcagta ttactcggcc agggaaaatg
     9961 atccgttatc ccgcaaggga aaaataatgg atactattga ttttgctgaa aaacgatttg
    10021 atacggcaaa gaattacctg ctgcaactga gagaaaaggg ggttatccgg gcaacccata
    10081 atgacaccaa actgaacaac atcctgttca gccgtcagaa tgaagcgctg tgcctgatag
    10141 atctcgatac ggtaatgccg ggatatgtcc attttgactt tggcgacgct ttgcggacca
    10201 tggccaatac agccctggaa gatgaacccg acctgaaacg ggttagattc aatgaaaaag
    10261 tttatcagtc ggtgacaaag gggttcctgg ctgaagcagg gccaatgctg aatgaggctg
    10321 aaaaagaatt attgccgtat gctcctgtat acatgacgtt tatcatcggt ctccggttcc
    10381 tcgctgatta tctgaacggg gatacctatt tccggattca ccacccggat cataatctgg
    10441 accgggccag ggtacagttt aaactggttc aggagataga gcaatttttg aaagcctgag
    10501 gctgttactc acaacggtca ccgagctgaa tgccatggct gccccggcaa tcatcggatc
    10561 gaggagcagc ccggtaaaag ggaaaagaac accggctgca acgggaatcc cgataatgtt
    10621 atagaagaaa gcccagaaca ggttttgccg gatgatccgc atgg
//
LOCUS       DAOQXS010000027        10623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6698_length_10623_cov_3.495931, whole genome shotgun sequence.
ACCESSION   DAOQXS010000027 DAOQXS010000000
VERSION     DAOQXS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_10623_cov_3.495931"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3189
                     /locus_tag="PKM34_01585"
     CDS             <1..3189
                     /locus_tag="PKM34_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNQ82302.1"
                     /translation="PNEAPNFRLKVEVLSADLGNLLSSDDLGIVNLQAELNGSGSSLN
                     DLKVDLNALVTGLEYRNYRYNEIVIDGTVTGKSFSGRLDVNDPNFKAAYEGTVDMNED
                     LPDLDFKLTVDKARLYETGLTTRSEDAMISGGLTVDIEGWNAEYFRGDIRVDNLHYLE
                     NGRHYTLKMLNVSRGRQEEKIDILTVRSDYVDADMTGKFNIRDLFRQIHGFIRNDESD
                     SLIFQEMENNPQVADLEVRIKDVNPITELFVPGMHTSPGIMLSGRFSSVDRILEAEGD
                     IRELIYSGMSADTIRLFAVYAFGSLYMDAGMRHLNLSEDQESEGIGIENLQLHTALTG
                     NDLYYAVQWNNDDPLKTNAGEFEGYARLLPDGAVETGLRNAQANINGHQWMISDLNII
                     RMDSASVGVKNFEIYGANEHFIIDGVLSHSAEDTLSLYFRNWELSNFNPILENYSLAL
                     GGEINGHFGLFRHEKKVNIFSALNVNDFTLNEVYLGEMDFDTRWIQSERALAVDLNLF
                     SEGNTNEHFKILGVNGVYYPMDKKRNFDFDIIAKNLDISVLKPFLTSFSSKLAGYATG
                     RLKLAGTNADPVVMGRLRLQRAEMNVDYLNVTYSFSNEVVFTENGILFNELQAYDPNS
                     QKALISGGIYHDHFRDMQMDITILPDQFMAINLNRYQNEVFYGKAYATGTVHLSGPFD
                     NLSISADVLTEKGSAITIPINYSVDVSENDFIIFSDSRDTVVDDNNREKQVSGLALDL
                     GMNITKDADIEIILPGNIGSIAANGDGNLRLGVDPYGYLNLTGSYVIRSGLFVFSLEQ
                     LVSRRFEILEGSSISWTGDIYDAEVSISARYRLRTDLNGLGISMIDPDAANQKVIVNT
                     DIRMSGNLFNPDLKFGITFPNMQEQTRQAVYAVLDTNDLGLMNQQAISLLVLGSFSST
                     GTGGSNLVNPASIVSNTLSNMLSQINNDFNIGINYMPGDQVSAEQLEVALSTQLLDDR
                     LIIDGNIDVSGSNANTQKTSSIVGDINIEYKLSPDGRFRVKAFNRSNDLSLFNDYAPF
                     TQGIGVFYRKEFDNLRDLFTPRKPKEKKVTRE"
     gene            3218..4312
                     /locus_tag="PKM34_01590"
     CDS             3218..4312
                     /locus_tag="PKM34_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82303.1"
                     /translation="MRRLILLSITLLVMPLLGLAQITITSEDMPVPGDTVRKSFTTFL
                     QGFDYQVGGADQTWYFDELTVVSQQVDTFVEVEETPAIYQLFFNNQFIYPDYNSTVAL
                     KMAEFTAIPGLVLSDSYQFIKVTDDEYREVGYGVNLEGVSLPIQLQEIDTIYRFPVEY
                     GDIDSAHSLLEIEVPDLGYLMVSKFRRNTVDGWGTLITPYGEFETLRMRTEITEYDSI
                     YSDSLGIGMPLLRNIIEYKWLANGYPEPLLVISEEPFLLTATYIDSVRTDFVSTPEII
                     KNDFQFSLYPNPANNHVSVEYELAGDADVQISLLSVYGIELKRFLSAQQEQGLYKKVI
                     YLKESGIPPGLYFLSLVIDQVPYTRKLIIN"
     gene            complement(4298..4813)
                     /gene="tnpA"
                     /locus_tag="PKM34_01595"
     CDS             complement(4298..4813)
                     /gene="tnpA"
                     /locus_tag="PKM34_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNQ82304.1"
                     /translation="MAYTYSQINIHAVFTVMGRENLISPAFRGHLFGYISETMKGIGV
                     YPLAVNGTADHVHIFFELPPNISVSKALQEVKASSSRWINNEKLVPGKFQWQKGFGAF
                     TNSRSQRDSVIKYILNQELHHAKNSFRDEYIELLQKYSVDFNPNNLFEFYVQDSENIL
                     PAETEGRLIND"
     gene            5078..>6882
                     /locus_tag="PKM34_01600"
     CDS             5078..>6882
                     /locus_tag="PKM34_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ82305.1"
                     /translation="MLSDQRNFIVYKSSAGSGKTFTLVREYLKIALKNPDAFRQILAI
                     TFTNKAANEMKERVVRYLMALAEPTIYPEPTAEKYLLPELSAFLKMDEDTIRKRAADV
                     LARIIHHYAEFAISTIDSFTHRVVRTFAHDLRIPMNFEVELDSDMILVQAVDILLSMA
                     GSEEQLTRVLVEFVEKKAGDEKSWQIENDLARFGKLLLSESSFGAVSELRKLDLSGFL
                     EIRKELHRYTGAFESQIRELAMDCIRLIRDAGIAPDDFNYKDKGVYGYLEKLSQGRYD
                     KLKPGVHASNAFTSGEWLNRKASQQARDAFEMISDRLNYFAGTLTTLLDKDLQTYMLY
                     RLILGQIYSTALLSVLEKILNGICEDNNKLLISEFNRRISSIVRDQPAPFIYERLGEK
                     YHHYLIDEFQDTSVMQWHNLLPLVENSLASGRMNLVVGDAKQAIYRWRSGDVEQFEKL
                     PRLIKSYPDPILNQRENALVENYMEKSLAQNHRSAPVIVDFNNRFFTFVSELLPPGYG
                     QSYRNAVQTVTGKEKPGLVKIQQVTASEDEESSYDDRMMEAIYKQISELRDDNFEWKD
                     IAILCRKNEKAAKIAEYLVSKKIPVISSESLLVAKS"
     assembly_gap    6883..6892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6893..8266
                     /locus_tag="PKM34_01605"
     CDS             <6893..8266
                     /locus_tag="PKM34_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ82306.1"
                     /translation="KVGFLLAWIRHLTDPEDEIQKVHILAYLKTNGYLLNTFLEDFFN
                     PKKDSPSSLFREVINGQEGFIGTEKLRTFEIYGLIRYISHYYNLGADDDIYLRFFQDA
                     VITFLGKERGGLPEFIDWWEEQQEKLSVIIPSGINAVRIMTIHKAKGLQFPVVIYPFA
                     DEKLRPGIDNIWVEIEDERLRPLRMACLPVAQALKETVYQQNFDDEMNRSLIDLVNVL
                     YVVMTRPEERLYILMPPPPEKTEDANSVPKLLNRFLKDGLQMTPSADVFLFGQRWKMP
                     SILSSSDEKENNLAHDGRPVLNMLLKRRAPESWDIENPDRNREWGKLVHLALSLITVK
                     DQTTRVLADLDSRGLIPHGKSDELKEQVLNVLNHPAMAPFFTPMCEVRNEPEIIAPDG
                     HLYRPDRVLINDGEAMLLDFKTGKKDESHKDQIARYGKLITEMGLQIRGGYLVYVQPV
                     PDIIKVL"
     gene            8387..>10623
                     /locus_tag="PKM34_01610"
     CDS             8387..>10623
                     /locus_tag="PKM34_01610"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HNQ82307.1"
                     /translation="MRNLILTMAALIIFPFAALTQDEARLLRFPSIHGDQVVFTYAGD
                     LYTVAKSGGMARKLTNHDGFEMFARFSPDGRQIAFTAQYDGNTEVYVMPAAGGIPVRL
                     TYTATLGRDDVSDRMGPNNMVMAWTPDGKYIVYRSRKQSYNDFVGQLFKVPVSGGLSE
                     EIPLSTGGFCSFSPDGSKLAFNRVMREFRTWKYYKGGMADDIRIFDFTTGEVVNITGN
                     DAQDIFPMWRGDNIYFLSDRDRTMNLFTYNIVDRQIRKLTNYTEYDIKFPSLGNNSII
                     YENGGFLYMFDLQSERPVKINVTIAEDFYGGRNELKDASKSISGADLSPDGKRVVFTA
                     RGDVFSVPAEEGITRNLTESSDAHDRSAAWSPDGKYIAYLSDQSGEYEIYIEAQDGSA
                     EPVKLTSDADTYKFNILWSPDSKKILWSDKKLRLQYVHIDTKEVKQVDKSQVWEFNDF
                     TWSPDSRWIAYVTQLPASNFGKILLFDTQSGEKTAVTDDWYTSNSPVFSYDGKYLFFA
                     SGRDFNPIYSETEWNHAYANMERIYFAILAADTPNPFAPKNDEVVIKEKDGKGDEGDK
                     GGKGDKGVAESEDGVVVTRIDAAGLGDRIVGLPVRPSNYFNINALDNKVYYNEMMAGG
                     DGVTLKLFDLKKKEETELGKGMFFSISYDGKKMLIAQQDKYAVIDLPQGKINISEFID
                     LSGLKTFVNKDEEWKQIFDEAWRQMRDFFYVENMHGVNWPAMKEKYGQMLPYVNNRND
                     LNYLIG"
BASE COUNT         2981 a         2256 c         2557 g         2819 t
ORIGIN      
        1 cccaacgaag caccgaattt tagactgaaa gtggaagtgt tatctgccga cctgggcaat
       61 ctgttgtctt ctgatgatct gggcatagtt aatttacagg cggaattaaa cggttcgggt
      121 tcctctttaa acgatctgaa agtcgacttg aatgcactgg tcacggggct ggaataccgc
      181 aattaccggt acaatgagat agttattgac ggtacggtga caggaaagtc attttcgggc
      241 cggctggatg tgaatgatcc gaattttaaa gcggcctatg agggaaccgt ggatatgaat
      301 gaggatctac ccgatcttga ctttaaactg actgtagata aagcccggtt atatgaaaca
      361 ggactgacaa cccggagtga agatgcaatg atctcgggtg gattgacggt cgatattgaa
      421 ggttggaatg cggaatattt tcgtggggat attcgggtgg ataacctgca ttatctggaa
      481 aacggcaggc attatacgct gaaaatgctg aatgtgagtc ggggaaggca ggaagagaag
      541 atcgatatcc tgacagtcag gtccgattat gttgatgccg atatgacggg taagttcaac
      601 atccgggatt tattccggca gatacacggt tttatcagga atgatgaatc agacagcctc
      661 atttttcagg agatggaaaa taatccccag gttgctgacc tggaagttcg gatcaaagat
      721 gtcaatccga tcactgaact ttttgttccc ggaatgcaca catcacccgg catcatgctg
      781 agtggccggt ttagttcggt ggaccggatt cttgaagcgg aaggcgatat cagagaactt
      841 atttatagtg gaatgtcagc cgacactatc cggttatttg cagtttatgc ttttggaagc
      901 ctttacatgg atgcaggtat gcggcatctg aatctttctg aagaccagga gagtgaagga
      961 attggtattg aaaatttgca gttgcacaca gctttgacgg gaaatgatct gtattatgcg
     1021 gttcaatgga ataatgatga tccgttaaag acaaatgccg gcgagttcga gggctatgcc
     1081 cgtttattgc ctgatggggc cgttgaaacc ggattgcgta atgcccaagc caatatcaat
     1141 ggccaccaat ggatgatatc tgatctgaac attattcgga tggattcggc atcggtaggg
     1201 gtcaaaaact ttgaaatata cggtgcaaat gagcatttta ttattgacgg agtactttcc
     1261 cattcggcag aagatacact cagcctttat ttcaggaatt gggaactctc gaacttcaat
     1321 cctatcctgg aaaactattc ccttgcactg ggcggggaga tcaatggcca tttcgggttg
     1381 ttccggcacg aaaagaaggt caatatcttt tcggcattga atgtcaatga ttttacgttg
     1441 aacgaggttt acctcggaga aatggatttc gatacacgct ggatccagtc ggaaagagca
     1501 ctggccgtcg atcttaatct tttttccgaa ggaaatacta acgagcattt taaaatattg
     1561 ggagtcaatg gcgtatacta tccaatggat aaaaagagaa attttgattt tgatataatt
     1621 gcaaaaaacc ttgatatctc agtattaaag ccatttctca ccagtttcag ttcaaaattg
     1681 gcaggctatg ccacaggccg tttaaaactg gccggtacaa atgcggaccc tgtcgtaatg
     1741 ggccggttgc gactgcaaag agctgaaatg aatgtggatt atctcaatgt tacgtattcg
     1801 ttttccaacg aagtcgtttt tactgagaac ggtatccttt ttaacgaatt acaggcgtat
     1861 gaccccaatt cacagaaagc gctgatctcc ggcggaatat atcatgacca tttccgtgat
     1921 atgcagatgg atatcactat cctgcccgac caattcatgg ccattaacct gaaccgttat
     1981 cagaacgagg tcttctacgg aaaagcttac gcaacaggga cggtacatct atcgggaccg
     2041 tttgataacc tctccatatc cgctgatgta ttgacggaga aaggctctgc cataacaata
     2101 ccgattaatt attctgtgga tgtatcggaa aatgatttca tcatattctc tgatagccgg
     2161 gatactgtgg ttgacgacaa taaccgggaa aagcaggtca gcgggctggc cctcgacctg
     2221 ggaatgaaca ttacgaaaga tgcagatatc gaaataatcc ttcccggcaa tattggcagt
     2281 attgctgcga atggagacgg caatctgcgg ctcggtgtcg acccgtatgg ctatctgaac
     2341 ctaacgggat cctatgtgat ccggtcggga ttatttgtgt tttcactgga acagcttgtc
     2401 agccggaggt ttgagatcct cgagggaagt tcgatcagct ggacgggaga tatttatgac
     2461 gccgaggtca gtatctctgc ccggtatcga ttgaggactg atttgaacgg actgggaatc
     2521 agtatgatag acccggatgc ggcaaatcag aaagtgattg tcaataccga catccggatg
     2581 agtggcaatt tgtttaatcc ggacttgaaa ttcgggatca cttttccgaa tatgcaggaa
     2641 cagacccggc aagcagtgta cgctgtgctg gataccaatg atctgggatt gatgaatcag
     2701 caagcgatat ccttactcgt ccttggaagt ttcagcagta cgggaaccgg tggcagtaac
     2761 ctggtcaacc ctgcgtcaat agtttctaat acgctgagca atatgttatc ccaaatcaac
     2821 aacgatttta atatcgggat taactatatg cccggcgacc aggtctctgc cgaacagctt
     2881 gaagtggcgc tttcaaccca gttgctggat gaccggttga ttatcgacgg aaatatcgat
     2941 gtttccggct ctaatgccaa tacccagaaa accagctcta ttgttggaga tataaacatt
     3001 gaatataaac tttcaccgga tggaaggttc agggtcaaag cttttaacag gtcaaacgac
     3061 ttgtcgcttt tcaatgatta tgcaccgttt acccagggga tcggcgtctt ttaccggaaa
     3121 gaatttgata atttacgtga tcttttcaca ccacgcaagc cgaaagagaa aaaagtaacc
     3181 cgggaataat ttcctctata tttgcaaaaa aaatcagatg cgacgactaa ttttgctaag
     3241 cattaccttg cttgttatgc ctctgttggg gctggcccag attactatta ccagtgaaga
     3301 tatgccggtg cccggcgata cggtgcggaa aagttttacc acttttcttc agggttttga
     3361 ctaccaggtc gggggtgctg atcagacatg gtatttcgat gagttgaccg tcgtgtcgca
     3421 gcaggtggat acatttgtgg aagttgaaga aacaccggcc atataccagt tgttcttcaa
     3481 taaccagttt atctatcccg attacaattc aaccgttgcc ctgaagatgg ctgaattcac
     3541 agccataccc ggactcgttt tgtcggactc gtatcagttt atcaaggtta ccgacgatga
     3601 ataccgggaa gtaggttatg gtgtaaacct tgaaggagtc tcgctgccaa ttcagttgca
     3661 ggagatcgat actatttacc ggtttcccgt cgagtatggc gatatcgatt cagcccattc
     3721 tttgcttgaa attgaagtgc ctgacctggg gtatctgatg gtgtcgaagt tcaggcgaaa
     3781 tacggtcgat gggtggggaa cactgataac tccctacggc gaatttgaaa cattgcggat
     3841 gagaactgaa attacggaat acgatagcat ttacagcgat tccctgggaa taggaatgcc
     3901 gctattaagg aatattatcg aatacaagtg gctggccaac gggtacccgg aaccattact
     3961 ggtcatcagc gaggaaccgt ttttgctgac agcaacctat atcgattcgg tccggactga
     4021 ctttgtgagc actcctgaga tcataaagaa tgattttcag ttttctctat atccaaaccc
     4081 ggccaataat catgtttctg ttgaatatga actggccggt gatgccgatg tacagatctc
     4141 gctcctttcg gtttacggaa ttgaactaaa acggttctta tcagcacaac aggagcaagg
     4201 cctttataaa aaggttattt acctgaaaga atcgggcatc cctccaggac tctacttttt
     4261 aagcttagta attgatcagg tgccctatac ccggaaatta atcattaatt aaacgccctt
     4321 ccgtttctgc cggcaaaata ttctcgctgt cctgaacata aaactcgaac aggttattcg
     4381 gattgaaatc aacgctatat ttttgcaaca gttcaatata ctcgtccctg aagctgtttt
     4441 ttgcatgatg taattcctga ttgagtatat atttaataac actgtctctt tgtgaccggc
     4501 tgttggtaaa tgcaccgaat cccttttgcc attggaattt gcccggaaca agtttctcat
     4561 tattgatcca ccttgaggaa ctggctttta cctcctggag agctttagaa actgaaatat
     4621 tgggtgggag ttcaaaaaaa atatggacat gatccgctgt accattgact gccaggggat
     4681 acaccccgat tcctttcata gtttctgaga tatacccaaa taagtgaccc ctgaacgcag
     4741 gcgatatcag attttccctg cccattactg taaatacagc atgaatgttg atttgtgaat
     4801 aggtgtatgc cataataatt aaattttagg gttaatgaat cgttttctat taatccgaaa
     4861 atcgacctga aaatacccgg tgaaagcaaa ataatttttt ataacactga aatacaaata
     4921 tttactaacg tccttctggg acgataatac ggtatcagac gaatccccca tcctttggag
     4981 tcccgtaggg acgccactat ggtaaaaaaa gaatccccca tcctttggag tcccgtaggg
     5041 acgccactat ggtaaaaaaa ggtaatttta tcccatcatg ttatcagacc agcgaaattt
     5101 cattgtctat aagtcatccg ccggatcggg gaaaacattt accctcgtgc gtgaatacct
     5161 gaaaatcgca ttaaagaacc ccgatgcttt ccgtcagatt ctggccatta cattcaccaa
     5221 caaagccgcc aatgagatga aggagcgcgt agtccgctac ctgatggcgc tggctgaacc
     5281 aaccatctac cccgagccaa ccgcagaaaa gtacctcctc cccgaattat ccgcattcct
     5341 gaagatggat gaggatacga tcaggaaaag ggctgccgat gtgctggccc ggatcattca
     5401 tcattacgcc gagtttgcca taagcactat cgatagtttt actcaccgtg tcgtgagaac
     5461 gtttgcccac gaccttcgca ttccaatgaa ttttgaggtg gaactcgact ctgatatgat
     5521 tcttgttcaa gccgtagata tactgcttag tatggctggt tccgaagagc aactgacccg
     5581 ggttctggta gaatttgtcg aaaagaaagc cggcgatgaa aaaagctggc aaattgaaaa
     5641 cgatctggcc agatttggca aattgctcct gtcggagtca agtttcgggg cggtcagcga
     5701 gttgcggaaa ctcgaccttt ccggctttct tgagatcagg aaagaattgc accggtatac
     5761 cggggctttt gaatctcaaa tccgtgaatt ggcaatggat tgtatacggt tgatccgtga
     5821 cgccggaatt gcaccggacg acttcaacta taaggataaa ggcgtgtatg gttacctgga
     5881 gaaattgtcg cagggccggt acgacaagct aaaaccgggt gtgcatgcct ccaatgcctt
     5941 cacttcaggt gaatggctga accgcaaagc gtcacaacag gcccgcgatg cctttgaaat
     6001 gatcagcgac cggcttaatt attttgcagg tacattgaca acactgctgg ataaggattt
     6061 gcagacttac atgctttacc ggttgatttt aggccagatt tattcaacag cgctgctttc
     6121 tgttctggaa aaaatcctta acgggatttg cgaagacaat aataaattgt tgatcagtga
     6181 gttcaatcgc cgtatctcct ccattgtcag ggatcagccg gctcctttta tttacgaaag
     6241 gctgggcgaa aaataccatc attacctgat agatgaattt caggatacct ccgtaatgca
     6301 atggcataac ctgctcccgc tggtggaaaa ctcgctcgct tccggaagaa tgaacctggt
     6361 tgtcggcgat gcaaagcagg caatttacag gtggcggtcg ggggatgtcg aacaatttga
     6421 aaaattaccc cgtcttatta agtcttaccc tgatccaata ctgaaccagc gggaaaacgc
     6481 cctcgtcgag aattatatgg agaaatcact ggcccaaaac caccggtcgg ctcctgtaat
     6541 cgtcgatttc aacaacaggt ttttcacctt cgtttccgaa ctgctgccac caggctatgg
     6601 ccagtcttac cggaatgcgg ttcagactgt tacgggaaaa gaaaagcccg gactggttaa
     6661 gattcagcag gtaaccgcat cagaagacga agaaagctcc tatgacgaca ggatgatgga
     6721 agcaatatat aagcaaatca gcgagttgcg cgatgataat ttcgaatgga aagatatcgc
     6781 aatcctttgc cggaagaatg aaaaagcggc aaaaattgcc gaataccttg tcagtaaaaa
     6841 aataccggtc atttcatccg aatccttgct ggtcgctaaa tcnnnnnnnn nnaaagtcgg
     6901 tttcctgttg gcctggatcc gccatttaac cgatccggaa gatgaaatcc agaaagttca
     6961 tatccttgcc tatttaaaaa ccaatgggta tcttctgaat actttccttg aagatttttt
     7021 taatccgaag aaagattcac cttcatccct gtttcgggaa gtgattaatg gtcaggaagg
     7081 attcatcgga acagagaaat taaggacttt tgagatatac ggactaatcc ggtatatatc
     7141 ccattattat aacctgggag ccgatgatga tatttatctc cggttcttcc aggatgccgt
     7201 cattacattt ctcggaaaag aaaggggagg gttgccggag tttatcgact ggtgggaaga
     7261 acagcaggaa aaactgtcgg tcattatccc ttcgggaatt aatgcggtca ggatcatgac
     7321 aatacataaa gcaaaggggc tacagttccc ggtagtcatt tacccttttg cggatgaaaa
     7381 actaaggcct ggtattgata atatctgggt cgaaatagaa gatgagcggc ttcggccact
     7441 ccggatggcc tgtctcccgg tcgcgcaggc ccttaaggaa accgtttacc agcaaaattt
     7501 cgatgatgaa atgaaccgat ccctcatcga tctggttaac gttctttatg ttgtcatgac
     7561 aaggccggaa gaaaggttgt atatccttat gccacctccg ccggagaaaa ccgaggatgc
     7621 caattctgtc ccgaaattat tgaaccggtt cctgaaagac gggcttcaaa tgacaccctc
     7681 agcggatgtt tttctgttcg gacaaaggtg gaaaatgccg tccatactgt cttccagcga
     7741 tgaaaaggaa aataatctgg cccatgatgg aagacctgta ttgaacatgc tgctgaaaag
     7801 gcgtgccccg gaatcgtggg atatcgagaa tccggaccgg aatcgggaat ggggaaaact
     7861 ggtacacctt gctttatcac tcattacagt taaagaccaa accaccaggg tgttagcaga
     7921 cctggattcg cggggtctaa tcccacacgg taaatcagac gaactaaagg aacaggtgct
     7981 taatgttctc aatcatccag caatggcccc atttttcacg cccatgtgtg aggtcaggaa
     8041 tgaaccggaa attatcgcac ctgacgggca tctgtatcgt cctgaccgtg tattgatcaa
     8101 cgatggtgaa gctatgctgc tggattttaa aacagggaaa aaagatgaat cacataaaga
     8161 ccagattgcc aggtacggca agctgatcac agagatgggt ttacagatac ggggtggcta
     8221 cctggtctat gtccagcccg tgcccgatat cataaaagtt ttgtaagaca attccgccca
     8281 ctcgtcaatc ggaaattacc aacggtgctt gttttgcggc aaaagggcat tttaccgggt
     8341 ttggtttaat ctttcatatt aatttagccg gtaaaaacat aacagtatga gaaatttgat
     8401 attgacaatg gctgccctga taattttccc ttttgcagcc cttacacagg atgaagccag
     8461 attactgaga ttcccctcaa ttcatggcga ccaggttgtt tttacctatg ccggagacct
     8521 ttatacggtt gcaaaatcag gtggaatggc ccgtaaactg actaaccatg acggttttga
     8581 aatgtttgcc cggttctcgc ctgatggcag acaaattgcc tttacagccc agtacgatgg
     8641 caataccgaa gtttatgtga tgcccgccgc aggtggaatc ccggtcaggc taacctatac
     8701 tgccacactc ggacgtgatg atgtttcaga caggatgggc ccgaataata tggtgatggc
     8761 atggacccct gacggaaaat atatcgtcta ccgttcacgc aagcaatctt ataacgattt
     8821 tgtgggacag ctatttaagg tgcctgtcag tggcgggcta tcggaagaaa ttccgctttc
     8881 gaccggcggc ttttgctcct tttcgccgga tggctccaaa ctggccttca accgcgtgat
     8941 gcgcgaattc cgtacgtgga aatactacaa gggcggaatg gccgacgata tcaggatctt
     9001 tgattttacg accggtgagg tagtcaatat caccggcaac gatgctcagg atattttccc
     9061 gatgtggcgc ggggataaca tctatttcct ttccgaccgc gacagaacga tgaacctttt
     9121 tacctataac atcgtcgacc ggcaaatcag gaaactcact aattatacag aatacgacat
     9181 taaattccct tcccttggaa ataattctat tatatatgaa aatggaggat ttttatatat
     9241 gtttgatttg cagtcggaaa ggcctgtaaa gattaatgtc accattgcag aagatttcta
     9301 tggcggtcgt aatgagctta aagacgcttc caaatcaatt tccggtgccg atttgtcgcc
     9361 tgacggcaaa agggttgttt tcacggctcg gggtgatgtt ttttcggtcc cggctgaaga
     9421 gggaataacg cgaaatctga ccgaaagttc cgacgctcac gatcgtagcg ctgcctggtc
     9481 gcccgatggg aaatatatcg catacctgtc cgatcaaagc ggtgaatatg agatatatat
     9541 cgaagcacag gatggaagcg cagaacctgt aaagcttact tctgatgccg atacctataa
     9601 gtttaatatc ctttggtcgc ccgacagtaa aaagatcctc tggagcgata aaaaattgcg
     9661 ccttcagtat gtccatatcg atacaaaaga agtgaaacag gttgataaat cacaggtatg
     9721 ggaattcaat gatttcacct ggtcgcccga cagccgctgg atagcctatg tgacgcagct
     9781 accagccagc aattttggta aaattcttct gttcgacacc caatcgggtg aaaagaccgc
     9841 tgtcaccgac gactggtata catctaacag ccctgttttc agctacgacg ggaagtactt
     9901 gttttttgca tccggaaggg actttaatcc gatatacagc gaaacggaat ggaaccatgc
     9961 atatgctaat atggagcgga tatattttgc catcctggcg gccgatacgc ctaacccgtt
    10021 tgcaccgaaa aatgatgaag tggtgattaa agagaaggat ggtaagggtg atgagggtga
    10081 taagggtggt aagggtgata agggtgtggc ggagagtgag gatggggttg tggtgacccg
    10141 gatcgatgcc gctggcttgg gagatcgtat tgtaggtttg ccggtcaggc cttccaacta
    10201 tttcaatatc aatgccctcg ataacaaagt ttattataat gaaatgatgg cagggggcga
    10261 tggcgttaca ctcaagcttt ttgacctgaa aaagaaggaa gagaccgaac tgggcaaagg
    10321 aatgtttttt agtatttcgt atgatggcaa gaaaatgctt atagctcagc aggataagta
    10381 cgccgttatc gacctgccac aagggaaaat caatatttcg gaatttatcg atctatccgg
    10441 gttgaaaact ttcgtgaaca aagatgaaga atggaaacag atctttgatg aggcctggcg
    10501 ccagatgcgc gatttcttct atgtggagaa tatgcatgga gtcaactggc cggcaatgaa
    10561 ggaaaaatac ggtcaaatgc tgccgtacgt caataaccgc aatgatctca attatctgat
    10621 agg
//
LOCUS       DAOQXS010000028        10323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_6937_length_10323_cov_4.134203, whole genome shotgun sequence.
ACCESSION   DAOQXS010000028 DAOQXS010000000
VERSION     DAOQXS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6937_length_10323_cov_4.134203"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKM34_01615"
     CDS             <1..233
                     /locus_tag="PKM34_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNQ82308.1"
                     /translation="GTVADPKVIFQHALKANASSLILLHNHPSNNTQPSEADIHLTKK
                     LVEGGKLLDIAVLDHLIVTTDGYYSFADEGLI"
     gene            342..746
                     /locus_tag="PKM34_01620"
     CDS             342..746
                     /locus_tag="PKM34_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82309.1"
                     /translation="MKDEISLSQMLAEMELSDLNGNPVKFSIIFVTADLKRGTGGEIY
                     IFGRRTPFVDRFRRQFKDSIFTESQVIKTVGKHNGQPLKSSQQAGDPRQKKDPRHYDH
                     KTINVMLLASKQVRKVHPDLILYFNDKEVIQN"
     gene            753..2084
                     /locus_tag="PKM34_01625"
     CDS             753..2084
                     /locus_tag="PKM34_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82310.1"
                     /translation="MSRRLIDDGFAITPGKTALITIRKDSSNPDGAKDTPAIPKSTAT
                     RDWSVWGDDNLFPQNVLADLEGNSIGLRALEKRRNVHFGRGIVAYRNVKGSDGTFTKE
                     IVNDGPVKEFFRLNQINLQWIDLIGGLEIFANGWIEFILNKAKTQINRCSILDPAYCR
                     HSMMDLKVLRIEKTFYSANWPLPSEEYLTAIPTFNPAKYEPGKPYPDAKFTYPVFYRS
                     FNKSYYSLSIWNGVRQSGWMAIANKVPALKKAIMENQMTIKYHVEIPDHYFAKRYPIS
                     DYTKEQRETEIQRKIEELNDFLTNVENSGKSLVTFSYYNEQLQKEFPGWQIHVIDNKL
                     KDDAYLPDSQAANSEILFAIGVDPSLIGASSVPGGKMASSGSGSDKREAFWSLNSECG
                     PYREITLQPLYFIRDFNKWGDDIEFDYLVVDTSQTQDQHPTKTEKRIDTNA"
     gene            2089..3507
                     /locus_tag="PKM34_01630"
     CDS             2089..3507
                     /locus_tag="PKM34_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82311.1"
                     /translation="MLFKESLNIDLAEIRQYLSVSQNGSFAKMRPHIAAAEILYIEDL
                     LGEALFNRLNEWYNSEESGSTSSSLSGSPALRSVLISGIPGESLATFSLTPQNIVGDI
                     NTTWIVNMMAHPSSQGTPDLIIENPKDGDHVYAILSNIGGDVQSNYMYIHVAESGEIT
                     IDEDEIIIIEDPFSMMMLKGEPILEGSGSTSPSLLIESPEALAGLLPLVQNALINFAY
                     YIGADEIGLHITDAGIQIITDETHKQAFQWQVEAAKSSWLNKAYLFSDVVLQFLEQHI
                     EDFPSWKSSEAYTENKDSLLYTTGQFNDEFFIRNSRRLFLALKPVIRSIESKYAVPTL
                     SQGYYDALLISIRSGSISADDQVILKKIRAAIAHLSMSEAITRFSVEIFPEGVYSNLV
                     SAFGTIMGKNPANKFDKAAAISSLASDGNAEIQAVQTYLDLNASESKYPLYFASSRYK
                     SPVDSPNRSEFKNDAAKGICLL"
     gene            3504..4394
                     /locus_tag="PKM34_01635"
     CDS             3504..4394
                     /locus_tag="PKM34_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82312.1"
                     /translation="MIDLSIEDKRYSLPESWQEITRKDLLIISDLYTRYFGSADFLKY
                     ALIRMLGIRRHLVRYRDQVAFAKGLYRVLPIPAIIHLGINRGKVKVFSQEDLLVLSHL
                     LSWIHSSTNTAKNSLTRNLLPRITVRRSLFSRLHLYGPTDQLRDVIAVEYAKADKAFL
                     AYCETGDPDQLDNLVGALYRPRKWYWRLERLFTDKDVSRRMRYTGVRYGRGLYSALSM
                     SLQVKYAVFLFFQGCRNDLLTKYPYVFTPDGGSDGHSTGWAGVFRALTNENIIDIDRV
                     MELPIHTVLFDLNEKIRLNK"
     gene            4414..4917
                     /locus_tag="PKM34_01640"
     CDS             4414..4917
                     /locus_tag="PKM34_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82313.1"
                     /translation="MADISTYIEYFEKLAKEHKEIQHSPTAKHFFMMDINELLSATNS
                     TAKYPAIVLLKLSGRAIDKKEDNRLIAIEGGFLVLDHCKNIDDFAAELAIFQKTFSIG
                     MQMIARIAHDQEPPVPYLSKAITDFDPDSVRWEMIGPVFENHFGMIFRFPVNLIADLG
                     YDSEKWS"
     gene            5050..7035
                     /locus_tag="PKM34_01645"
     CDS             5050..7035
                     /locus_tag="PKM34_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNQ82314.1"
                     /translation="MITIQFVLTNSIYAQATSWEENQLDGSQHKFYIGGLTKNSLEFA
                     DIDGDNDYDCFVTTNALDNINSLYENVVYFENVGDSLSPVWELISTAYLDNDIPSLFG
                     LKVRFVDIDSDCDLDMFIGGVSIPPLLYYKNIGSKYSPEWELVPGFLSNLEEDEMTKY
                     CYPAFVDIDGDDDYDLIYGNYYGDDIYYENVGDKYNCDFIKKQTEYFGSSNLGHCNNL
                     EFYDIDGDSDFDALIGRDYSLLYMQNIGTIDSAIWQVNTTNYLGSNWLADFYSPSFAD
                     LDNNDTSELFFGTENGIIWTYNDTANHWIKYDDLYFDDGSYLNPEFADLDGDGNQELI
                     IPMYDFHKDSSFIKIYTNIGNADSIVWDISNIVIQVEYPYPLNRVTFADIDADGDLDM
                     IAGFQDFNLDIILFKNTGDLYTPVFQEEYEVIATFQEEELIQFFPVLVDYDNDHDLDI
                     IVSAQDGTTNSWEWVDFFTNTGDSIYYHWEYSYTQKLGYGAICCYDDDLDGDLDMMFS
                     FWNHITLVKNIGNKWDPIFQPPHIEKIEIPDQWLWGIALTDLNNDGKEDLIIGTEYGG
                     MYRYDNMGIVYNVNSIDKEVMTIYPNPAHSDLFISGIEFNKGKYCVSLFNSTGKFLEE
                     FKLTKTHFSVSGYAPGIYLYSISLDGKTIKSGKFIIK"
     gene            7130..7564
                     /locus_tag="PKM34_01650"
     CDS             7130..7564
                     /locus_tag="PKM34_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82315.1"
                     /translation="MTDTELQWRFIRARLIGHGMLLSEKLQEGILTQQLIDSRKLINS
                     LQLHVNMDESVAVGELVIEFKIYGRFQEIGAGRHPKNNTDFLDSFIGARKTRRKRPWY
                     NKTVYGTLNYLIYDLVNGFTDETKEMLKAQILRYDKFISSHG"
     gene            7557..>10323
                     /locus_tag="PKM34_01655"
     CDS             7557..>10323
                     /locus_tag="PKM34_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNQ82316.1"
                     /translation="MGKITDDKINLTISVNGTAAKNELGKLEKEAVILSKSYKQLILD
                     KKNLDKTDKDYKSNLSEVNKKLTQTKVAVEQNKVAQDNLRKSIGLTGMTMNQLNKEAM
                     KLRAMKANLTPGTEQFKKINKELEETNAQIRKLKTGAASQGGGTFGKLADSFNKYQAL
                     AMGAVAAIAGVGLAIGGIIKGNAELTDSLADVRKTTGMTEEEVKRFNSRLKNIDTRSS
                     RKELLELAVVAGKLGISGTKDVFEFVRAADQIGVALSKDLGGNTEDAVNSLGKLTELF
                     GLKAEFGLEQSLLKTGSAINALGAASTASEAYLVNFSKRMGGVGKQADVSLQNILGYG
                     AALDQLGQTAEVSTTTLSKVWVNMFKDPAEYANIAGMAVSDFSNLLKTDANEAFLKFL
                     EGLNGNNAGLGVMSKKMEALKLDGARSVGVLSSLAGNIELVRKQQVISNDEFTKGTSL
                     TKEFNVKNNNLAATLEKLGKRLSSLFVNPQLNSSLTDIVNTLYKWMKIPVDAALRAEQ
                     KEVNRLVFELTKANTKEEDRQKILAELQKINPKIVEGLSAEKIETGKLVSNLRMYNEE
                     MAKKIVLANLEESEQENAAKEAKWLGKRANAEYDLMVLLQKYDAQIALNSELSLEEKY
                     NRIFAIMQEARKKEIAEGVKGEQIYNPSTEQYYYGETQVESEQAQAIAAYKNVIAYEA
                     EYQQALKNGIDFSERIAAIKKMLNLSPSGDNTEIFGGSGSTSTVSGGGGGGDDLTKSQ
                     QKELDKIKEYREEVLKGAMSLIEQERLAYDERLRQAGIYGKKQEEMTEEDLAVQKALM
                     EQHIANIKKINDDAFTKDIARMQEEFDLETTRRQTAYNEQLKIIGDNEAEKAAFKQSF
                     DKQEEERTREHLEELLNILQDQLPGKDDFNIDLEDAVLSDEAKASLLKKIEEVKAQLA
                     ALGLPTGD"
BASE COUNT         3021 a         2199 c         2238 g         2865 t
ORIGIN      
        1 ccggtactgt cgctgatccc aaagtcatct tccagcatgc cctcaaagca aatgccagct
       61 ccttgatcct gttgcacaac catccttcaa ataataccca accttctgaa gcagatattc
      121 acctgaccaa gaagctggtt gagggcggca aactgctgga tatcgctgtc ctggatcacc
      181 tgatcgtcac caccgatggt tattattcct tcgccgatga aggcttgata taacaaccac
      241 cgcttcaacc taagggagtc cggcgaagcc gaactccctt tttttgctcg ttccggtttt
      301 gtcctttcct aatcgctgtt ctgtttcttt ctttgtccat tatgaaggat gaaataagtt
      361 tatctcaaat gcttgctgaa atggagcttt cggatttaaa tgggaatccg gtaaagttct
      421 ccatcatttt tgtgacggct gatctgaaaa gaggaaccgg tggagagatt tacatctttg
      481 gtcgcagaac tccctttgta gacaggtttc gccgtcaatt taaggactcc atttttacag
      541 aaagccaggt cattaaaacc gttggtaaac ataatggaca acccctcaag agcagtcagc
      601 aagccggtga tcccagacaa aaaaaagacc cccgtcacta tgatcataag acgatcaatg
      661 tgatgctgct ggcttcaaaa caggtccgga aagttcatcc ggatctgata ttgtatttta
      721 acgataaaga agtaatccaa aattagaatt caatgtccag aagacttata gatgatggct
      781 tcgccattac tccgggcaaa acagccctga tcaccattcg caaagattct tcaaatcccg
      841 atggagccaa agacactccg gccatcccta aatcgactgc aaccagggac tggtctgtat
      901 ggggagatga caaccttttc ccgcaaaatg tcctggctga cctggaagga aattctatcg
      961 gtctccgggc gctggaaaaa cgcaggaatg tccattttgg ccggggaatc gttgcttatc
     1021 gcaatgtcaa aggctctgac ggcacgttta caaaagaaat cgtcaatgat ggtccggtta
     1081 aagagttctt ccggctgaat cagatcaacc tgcaatggat cgacctgatt ggcggattag
     1141 agatatttgc gaacggatgg attgaattca ttctcaataa ggcaaaaact cagatcaatc
     1201 ggtgctcgat cttagatcct gcctactgcc gtcattccat gatggattta aaggttctca
     1261 gaatagagaa aactttctat tctgccaact ggcctttgcc ctcagaggaa tacctgaccg
     1321 caataccgac tttcaatcct gcaaaatatg agccgggcaa accgtatccg gatgctaagt
     1381 ttacttaccc ggtcttttat cgttccttca ataaatccta ctattccctg tcaatctgga
     1441 atggtgtccg gcaaagcggc tggatggcaa ttgccaacaa ggtgccggcc ttgaaaaaag
     1501 ccattatgga aaaccagatg accataaaat accatgtcga gatcccggat cattattttg
     1561 caaagcgata cccgatttcc gattatacga aagagcagcg ggaaacggag attcagcgca
     1621 aaatcgagga attgaacgac tttctaacga atgtcgaaaa ctcagggaaa tcgcttgtta
     1681 ccttttctta ttataatgag cagctccaaa aggaatttcc cggctggcaa attcatgtga
     1741 tcgataacaa gctcaaagat gatgcttacc tgccggactc acaggccgca aacagtgaaa
     1801 tactttttgc catcggcgtt gatccgtccc tgatcggtgc atccagcgtc cccggtggaa
     1861 aaatggcttc ctccggctcc ggttcggata aaagagaggc tttctggtcc ctcaattccg
     1921 aatgtggccc atatcgtgaa attaccctgc agcctcttta tttcatccgt gactttaaca
     1981 aatggggtga tgatattgaa tttgattacc tggtggtcga tacttcccag acccaggacc
     2041 aacacccgac taaaactgaa aaacgaatcg atacaaacgc ttaatgctat gctattcaaa
     2101 gagtctttaa atattgacct tgccgagatc aggcagtatc tctctgtttc tcagaacggc
     2161 agttttgcca aaatgagacc gcatatcgca gccgctgaaa tcttgtatat cgaagacctt
     2221 cttggggaag ctctttttaa ccggctgaat gaatggtata attcagaaga atccggctca
     2281 acttcttctt ctctgtcggg aagccctgct ttacgttcgg ttttgatcag tggcataccg
     2341 ggtgagagcc ttgcaacctt ttcgctcacg cctcaaaata tcgtaggcga tataaataca
     2401 acctggattg tgaatatgat ggcgcaccca tcttcgcagg gcactcccga cctaatcatt
     2461 gaaaatccca aggatggaga ccatgtctat gccatactca gcaatatcgg cggggatgtt
     2521 caatcgaatt acatgtatat tcatgttgct gaatccggcg aaataaccat cgatgaagat
     2581 gaaatcatca tcattgaaga tcctttctca atgatgatgt tgaaaggaga accaattctt
     2641 gaaggttccg gttcgacttc tccttcatta ttgattgaat caccggaagc tcttgccggt
     2701 cttcttcccc tggtgcaaaa tgccctgatc aatttcgcct actacatagg cgctgatgaa
     2761 ataggtttgc atattacgga tgccggtatt caaatcatca cggatgaaac ccataaacag
     2821 gcctttcaat ggcaggttga agccgctaaa tcaagctggc ttaataaagc ttatcttttc
     2881 tccgatgtag tcctgcaatt cctggaacaa cacattgaag actttccttc ctggaagtcc
     2941 tcagaggcct ataccgaaaa caaagattct ctgctctata caaccggcca gtttaatgat
     3001 gaattcttca tccggaacag ccgccggctt ttccttgccc ttaaaccggt cattcgcagc
     3061 attgaatcaa aatacgcagt tcctaccttg tctcaaggct attatgatgc cttgctgatt
     3121 tcaattcgct ctggctccat ttcagcagac gaccaggtca tcctcaaaaa gatcagagct
     3181 gccattgccc acctgtccat gtcggaagct attaccaggt tttccgttga aatcttcccg
     3241 gaaggggtgt actcaaacct tgtttctgca ttcggcacca tcatgggcaa gaatccggcc
     3301 aataagtttg acaaagctgc cgccatttcc tcattggcct cggatggcaa tgcagaaatt
     3361 caggcggttc agacctatct tgaccttaat gcttcagaga gcaaataccc gttgtacttc
     3421 gcttcatcca ggtataaaag tccggtggac tctccaaacc gttctgaatt taagaatgat
     3481 gccgcaaagg gaatctgcct gttatgatcg atctatccat agaagacaaa agatattccc
     3541 tgccggagtc ctggcaggaa attacccgaa aggatctgct gatcatttcg gatctctaca
     3601 cccgttattt cggttctgcc gactttctta aatacgcact cattcgtatg cttggcatac
     3661 gccgtcacct tgttcgctat cgtgatcagg ttgcctttgc gaaaggactc taccgggttc
     3721 ttcccattcc ggcaatcatt cacctgggaa taaatagggg caaggtcaag gtattctcgc
     3781 aggaggatct tctggttctc tcacatttac tttcatggat tcattcatca accaacactg
     3841 ctaagaattc gctcaccaga aacctcctgc cgagaatcac cgttcgccgt tcattgtttt
     3901 ccaggcttca cctatacggt cctaccgatc agttaaggga tgtcatcgct gttgaatatg
     3961 cgaaggctga taaagccttc cttgcctatt gtgaaactgg agatcctgac cagcttgaca
     4021 atcttgtagg tgccttatac cgaccccgaa aatggtactg gcggttggaa aggctattta
     4081 ccgataaaga tgtctccagg agaatgcgct ataccggtgt gaggtacggc agaggattat
     4141 atagcgcact ctcaatgagt ttacaggtta aatacgccgt ctttttgttc ttccagggtt
     4201 gccgcaatga ccttttaacg aaatatcctt atgtctttac ccctgatgga ggttcagacg
     4261 ggcattccac cggttgggcc ggtgtctttc gtgccttgac caatgaaaat attatcgaca
     4321 tcgaccgggt gatggagctg cccatccaca cagttctgtt cgacctgaat gaaaaaatcc
     4381 ggctgaataa ataaaaccag aatcctaaaa ccaatggctg atatttcgac ttatattgaa
     4441 tactttgaaa agcttgcgaa agagcataag gagattcaac attctcctac tgccaagcat
     4501 ttcttcatga tggacattaa cgagttattg tctgcaacca attcaacggc taaatatccg
     4561 gccattgtcc tgcttaaact gtcaggtaga gctatcgaca agaaagaaga taatcggcta
     4621 attgccattg aaggcgggtt cctggtgctc gatcattgca agaacattga cgacttcgct
     4681 gcagaacttg cgatttttca aaagaccttt tccattggca tgcaaatgat tgcacggatt
     4741 gcacacgatc aggagccacc ggttccttat ttgtcaaaag cgattactga ctttgatcct
     4801 gattctgttc gatgggagat gatcgggccg gtttttgaga atcacttcgg gatgattttc
     4861 aggtttccgg tgaatctgat tgctgacctg gggtacgatt ctgagaaatg gagctaaggg
     4921 tatttttata atcaattttt aaagagtttc tagcattagc gttattttat gtaaatttac
     4981 cctaaatcct tattatccta ttaaaaatta ttacaattga aattatcagg cttcttaatt
     5041 ttaatcattt tgataacgat tcagtttgtt cttactaatt ctatttacgc tcaggccact
     5101 tcttgggagg aaaatcaatt agatggaagt cagcataaat tttatatcgg tggtttaacg
     5161 aagaattccc ttgaatttgc tgatattgac ggtgataatg attatgattg ttttgttacc
     5221 actaatgctt tagacaatat aaattcattg tatgaaaatg ttgtttattt tgaaaatgta
     5281 ggggattcat tatcaccggt ttgggaactg atctcaacag cgtatcttga taatgatatt
     5341 ccgtcccttt ttggccttaa agtccggttt gtggatattg actccgattg tgaccttgac
     5401 atgtttatag gcggcgtttc gattccgcct ttgttgtatt ataagaatat aggatcgaag
     5461 tatagcccgg aatgggaact ggttccaggc tttttgagta atcttgaaga ggatgaaatg
     5521 acgaaatatt gttatcctgc atttgtagat atcgatggag atgatgacta tgatttaatt
     5581 tacgggaact attatggcga tgatatttat tacgaaaatg ttggcgataa gtacaattgt
     5641 gatttcatca agaaacaaac ggaatatttc ggttcttcca atttagggca ctgcaataat
     5701 cttgaatttt atgatattga tggcgattcc gactttgatg ctttgatcgg tagggattac
     5761 agccttttat atatgcaaaa tataggaacc attgactctg ccatctggca ggtgaatacg
     5821 accaattatt taggtagtaa ttggcttgcc gatttttata gcccatcctt tgcagatctt
     5881 gataataatg atacttcaga gctttttttt ggtactgaaa atggcatcat ctggacttat
     5941 aatgacacag cgaatcattg gatcaaatat gatgatcttt atttcgatga tggttcctat
     6001 ctgaatccgg aattcgcaga tttagatgga gatggaaatc aggaattaat catccccatg
     6061 tatgattttc ataaagattc ttcttttatt aaaatataca ctaacattgg aaatgctgat
     6121 tctatcgttt gggatatttc caatatagtt atacaggttg agtatcctta tccgcttaac
     6181 cgtgttactt ttgctgatat tgatgccgat ggtgatcttg acatgattgc cggatttcag
     6241 gatttcaacc ttgatataat tctctttaaa aataccggag acttgtatac ccctgttttc
     6301 caggaagaat acgaagtcat tgctactttt caggaagaag aactaattca atttttcccc
     6361 gttttggtcg attatgataa tgaccatgac ttggatataa tagtcagcgc ccaagatggc
     6421 accaccaatt catgggaatg ggttgatttt tttactaata cgggtgattc aatttactat
     6481 cactgggaat attcttacac acaaaaatta ggttacggcg ctatttgttg ttatgacgat
     6541 gatttggacg gtgacctgga catgatgttt agtttctgga accatattac acttgtgaaa
     6601 aatataggaa ataaatggga tccaatattt caacctcctc atattgaaaa aattgaaatc
     6661 cctgaccaat ggctctgggg catcgcatta actgatttaa ataatgatgg aaaagaagac
     6721 ttaattattg gaaccgaata tggaggcatg tacaggtatg acaatatggg gatagtatat
     6781 aatgtgaatt cgatagataa ggaagttatg acaatctatc ctaatccggc acacagcgat
     6841 cttttcatct caggaataga atttaataaa ggtaaatact gtgtgagtct tttcaattcg
     6901 actgggaaat ttcttgaaga atttaaattg accaaaacgc atttttccgt ttcaggttat
     6961 gcccccggta tttacctata tagcatttct ctagatggta aaaccatcaa atccggaaag
     7021 tttataataa agtagctcgt ctgtcttgat cattacaaaa ggaaactaaa aattgccttt
     7081 ttgatctgtc ctttgtcgct cgcctgagta acaccatttt tgtcctgtta tgacagatac
     7141 agaacttcag tggcgtttta tccgggcaag gctcattggt catggaatgc tgctctcaga
     7201 gaaacttcag gaaggtattc tcactcaaca acttattgac agccgtaaac tcataaactc
     7261 gcttcaattg catgtcaata tggatgaaag tgtcgcagtc ggtgaattgg tcatagaatt
     7321 taagatttat ggccgctttc aggagatcgg cgctggccgc catccaaaaa ataatactga
     7381 ctttcttgat tctttcatcg gtgccaggaa aacacgccgg aaacgcccct ggtataacaa
     7441 gaccgtttac ggcaccctca attacctgat ctatgacctg gtcaatggtt ttaccgatga
     7501 aaccaaagaa atgcttaaag ctcaaatcct tcgttacgac aaattcataa gttcacatgg
     7561 gtaaaattac tgatgataaa atcaatctta ccatttccgt caatggtacg gctgctaaga
     7621 atgagttagg caaactcgaa aaggaggcgg taattctttc aaagtcttat aagcagctta
     7681 tcctcgataa aaagaatctc gataagacag ataaagatta caagagcaac ctttccgaag
     7741 tcaataaaaa attgactcag accaaagtgg ctgttgagca aaacaaagtt gcccaggata
     7801 accttcgtaa gtccattggc ctaactggta tgaccatgaa ccagctcaat aaagaagcca
     7861 tgaagctccg ggcaatgaaa gctaatctga ctcccggaac tgagcaattt aaaaaaatca
     7921 acaaggagct ggaggagacc aatgcgcaga tccgcaaact caaaaccggt gctgcttccc
     7981 aggggggagg tacattcggc aaactggcag actccttcaa taagtaccag gctcttgcaa
     8041 tgggcgctgt tgctgccatt gccggagttg gtctagccat tgggggcatt attaaaggta
     8101 acgcggaatt gactgattcc ctggctgatg ttcgcaaaac aaccggaatg acggaagaag
     8161 aagtcaaacg ctttaatagc cggcttaaaa atatcgatac ccgttcatcc aggaaggaat
     8221 tgcttgaact tgcagtagtg gccggtaaat tgggtatctc aggcaccaaa gatgttttcg
     8281 aatttgtccg ggcggctgat caaatcggcg tggctctcag taaggacctg ggaggcaata
     8341 ccgaggatgc cgtgaattct cttggaaagc ttaccgaatt gttcggcctt aaagctgaat
     8401 ttggccttga acaaagtctt ctgaaaacag gatcggcaat caatgcgctt ggcgctgctt
     8461 caacagccag cgaagcatac ctcgttaact tctcaaaacg catgggaggt gttggcaaac
     8521 aagccgatgt ttcactgcag aacatcttgg gctatggtgc cgcacttgat cagttaggcc
     8581 agaccgcaga agtatcaaca accaccttgt caaaggtttg ggtcaacatg tttaaagatc
     8641 cagccgaata tgccaatatc gcgggaatgg cggtgagtga cttttcaaat ctcttgaaaa
     8701 ccgatgccaa tgaagcgttc ctcaaattcc tggaagggtt gaacggaaac aacgccgggc
     8761 ttggggtgat gtcaaaaaaa atggaggctc tgaaacttga cggggccagg tctgttggtg
     8821 ttctttcttc actggccggc aatatcgagt tggtcagaaa acagcaggtc atctcaaatg
     8881 atgaatttac caaaggaacc agcctgacca aagaattcaa tgtcaaaaac aataaccttg
     8941 ccgctaccct ggaaaaactt ggcaagcgct tgagttcctt gtttgtaaat ccgcaactta
     9001 acagttcgct taccgatatc gtcaacacgc tttacaaatg gatgaaaatt cctgtcgatg
     9061 ctgcgctccg tgcagagcaa aaagaggtaa accggcttgt ttttgagctt accaaagcca
     9121 atacaaaaga ggaagaccgg cagaagatcc tggcagaact gcagaaaata aacccgaaaa
     9181 tcgttgaagg gctttctgcc gaaaagattg aaacgggtaa actggtttcg aacctcagaa
     9241 tgtataatga ggaaatggca aaaaagattg tcctggccaa tcttgaagaa tctgagcagg
     9301 aaaatgctgc caaagaagca aaatggctgg gaaaacgtgc caatgctgag tatgacctga
     9361 tggtcctgtt gcaaaaatac gatgctcaaa tcgctcttaa ttcagagctt tcgctggaag
     9421 aaaaatacaa tcgcattttt gccatcatgc aggaagccag gaaaaaagaa attgctgaag
     9481 gagtgaaagg agaacagatt tacaatcctt ccaccgagca atattattat ggagaaacac
     9541 aggttgaaag tgaacaggct caggcgatag cggcgtataa aaatgtaatc gcatacgaag
     9601 ccgaatacca gcaggctttg aaaaatggga ttgacttcag cgaacgaatt gctgccatca
     9661 agaaaatgtt aaatctttct ccttccgggg ataatactga gatttttggc ggaagcggct
     9721 caacttccac cgtctccggg ggtggcggtg gtggtgacga tttgaccaaa tcacaacaaa
     9781 aagaactcga taaaatcaag gaatatcgtg aagaagtcct caaaggcgct atgtccctga
     9841 tcgagcagga acgtcttgcc tatgatgaaa gactccggca ggcagggatc tatggcaaaa
     9901 aacaggagga aatgaccgaa gaagatcttg cagttcagaa agcgctgatg gaacagcata
     9961 ttgccaatat caaaaagatc aatgatgatg ctttcacaaa ggatattgca aggatgcagg
    10021 aggaatttga cctggaaacc acacgccgtc agaccgccta taatgagcag cttaaaatta
    10081 tcggggataa tgaagcggaa aaagccgcat tcaaacaaag ctttgataaa caggaggaag
    10141 aacggacccg ggaacatctt gaggagcttc tgaatatttt acaagatcaa cttcctggta
    10201 aggatgattt taatattgat cttgaagatg cggtgctttc cgatgaagct aaagcttctc
    10261 ttttaaagaa aattgaagaa gttaaggcac agttggctgc gttgggcttg ccaaccggcg
    10321 ata
//
LOCUS       DAOQXS010000029        10101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7128_length_10101_cov_3.764483, whole genome shotgun sequence.
ACCESSION   DAOQXS010000029 DAOQXS010000000
VERSION     DAOQXS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_10101_cov_3.764483"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKM34_01660"
     CDS             complement(<1..343)
                     /locus_tag="PKM34_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82317.1"
                     /translation="MRKILPVFILTLMVITSAAQDTLIVQTFTFDSITTRRGTWQFPE
                     GESFRKILMFHTLKCDFATTHDQYACGEWDYLTYNTVYRHTGAYDSTLYHHPNFTLVE
                     GTAPDSLLLTSS"
     gene            575..1213
                     /locus_tag="PKM34_01665"
     CDS             575..1213
                     /locus_tag="PKM34_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82318.1"
                     /translation="MKKLLLIVVLVPALFLTSCFKEKAGELGGDQSPMGEVGVTVSSS
                     SMEIAGVSNFTATVTALKDGVSSYTATATVTNPLLKNIAANYPGVEVNGDQVSITNVK
                     IQQTKEGIKSMEGPGAGVLVKYDSKVGDTYPIDGTDRERKVVSKSTDDDYYYGFFLIK
                     TIQVEMDQSHVKSSGITKYTYIANHKFGLVGVKVDFDDQTSVTFPIYTSAEN"
     gene            1247..1690
                     /locus_tag="PKM34_01670"
     CDS             1247..1690
                     /locus_tag="PKM34_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82319.1"
                     /translation="MGSFLLPYRWKIAGIVMAIAGMAGAAVYGFFDFRFKVPVFAVVS
                     IFVEPKFFTVFQTNFSEEVVLFFLITGLNLIIFSKEKEEGEDLERIRLKAFARAMITN
                     AAVQLFAVLFVYGAGFAAVMVFNLLSVNILYLIFFYRMRWSLLKN"
     gene            1752..2510
                     /locus_tag="PKM34_01675"
     CDS             1752..2510
                     /locus_tag="PKM34_01675"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HNQ82320.1"
                     /translation="MNRIIFISLLLITAIIPTWAQTPGTLTVSVSTSAAGGNYAPKNI
                     VAIWIEDNQGNFVKTLLAYAQTRKTHLNTWEASTTAAGSPYNTVDAITGATKTSHATR
                     TCSWDGTDINGNIVSDGTYKLRMELTDKNGTGNFSTFTFEKDTLQVNLTPANVPSFSS
                     IVINWEPVITAIGEPETAGSITIYPNPSSGVINLTGKYDEVIVFGTSGRQIVKSAGNK
                     IDLTASPDGLYFVHVRTGVETEIFKVVKTNDLEK"
     gene            2494..2862
                     /locus_tag="PKM34_01680"
     CDS             2494..2862
                     /locus_tag="PKM34_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNQ82321.1"
                     /translation="MTSKNKPSPSSDWKSRLGVVYSTNPDYSYATGESAEPGTLPPQQ
                     QNLRVSLDRKQRKGKSVTLIQGFIGRDEDLELLAKQLKSKFGVGGSAKEGEIIIQGDF
                     VVKTIEFLQNLHYKVKRSGG"
     gene            2959..4584
                     /locus_tag="PKM34_01685"
     CDS             2959..4584
                     /locus_tag="PKM34_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNQ82322.1"
                     /translation="MTKSYLYFLILMIPAGLFAQVSPDWSMIYNGNDDYFDNGVAIAL
                     DPSGNVFVGGTSVSITNGAPDYVVLKYTSAGEFKWSYEYDGTGAYEDFLAAITTDSEG
                     NVYATGGSHEGSSRRDYVTLKIDGSGAFAWKSIYKGLDVNNDDAAADIVVDENGYVYV
                     TGASAGYSSSMWTDFATLKLTPGGDTVWTRRLGGPGMPNDRATAIAVDNFGYVYVTGY
                     FEGDVSTRYNYATAKYKPNGDTAWVRVYNGTGSDEDRAYAIAVDENRNVYVTGSSWGL
                     NADDYATVKYDSNGVQKWVARYNGPAGSYDKAWDLKLDDEGNVYVTGQSGGTSGNYDY
                     CTIRYNPDGGEDWVARYNGPDNLDDVATSVAIDPSGNIFVTGYSYNPPSPYISTTDMV
                     LIKYNPEGLEEWVSRFDGPGNNYDQGYAIAADPSGDVFVTGSTEYYGQQNSRDIFTVK
                     YAGPSLVEDFSSGNSINLYPNPADHQLTVQVPCGAALISVCDLTGRQIIVHELKVSEK
                     DEQTFNISGIPSGAYIVKVTTTLGISEKKLIVY"
     gene            4595..4987
                     /locus_tag="PKM34_01690"
     CDS             4595..4987
                     /locus_tag="PKM34_01690"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNQ82323.1"
                     /translation="MVKMTAVYHSADEYITCQPEQFRANLALIRQTIREAVPDATEAI
                     SYQMPAFKFHGILCFYAVFKDHYSLFVSPEIHKAFVEELKSYKTTKSAIHFPFDRPVP
                     VELIVQVVRFAAITNLEKAEARLKKRKR"
     gene            5123..6340
                     /gene="ilvA"
                     /locus_tag="PKM34_01695"
     CDS             5123..6340
                     /gene="ilvA"
                     /locus_tag="PKM34_01695"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNQ82324.1"
                     /translation="MLTLPDYSDIIKAQMRLKGVVKNTPLEPSKSFSAMSGAHIYLKL
                     ENFQSTGSFKVRGAYNKISSLDPGELANGVVCASAGNHAQGVAFAARALGLRATVFMP
                     VFTPPLKVIATRSYGADVKLEGETFDDALSAAKKFQESSGAVFIHAFDDPLIIAGQGT
                     IGLELFQALETIQDVVVPVGGGGLIAGIAIALKKTNPDIRIIGVEAEGAASMQTSLRD
                     GKLTRLTSMSTIADGIAVKTPGELTFAAAKQFVDEIVTVSDAEIAHAAYLLLQRAKLL
                     AEPAGVASMAAVLHKKLDFKNRQVVTVISGGNINMGLLEQILEKGMMAEGLRARIAVL
                     IPDMPGELKSVITILENLFANINEISHERSITTVPVGHVLVTITFNLQQIEQLDMVCK
                     ALEGKMKKFEVLR"
     gene            6481..7704
                     /locus_tag="PKM34_01700"
     CDS             6481..7704
                     /locus_tag="PKM34_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNQ82325.1"
                     /translation="MSLKIKLPALHWQILIALALAVLFGIYFSEYVKYVSWMGTIFLR
                     ALQMIIMPLVISSMICGVANIGDAQNLGRIGLKTMLFYVITTVIAILTGLVLVNFFQP
                     GVGADLNLIEKAEIPAASGSLGQILIDIVPENVFAVMSVNRQLLSVILFALLFGFFII
                     KVSEKPRKQLTDFFNAVFEVMMKITFFIIKFTPLGIFGLVAEKIAPHKDVAALFESLG
                     IYMVVVLLGLFIHAVVFMPLIIRFIGRINPLRHFNAVKTALFTAFSTSSSNATLPLTM
                     ESIEVNAGVSNKITSFTLPLGATINMNGTALYECVAVIFIAQAYGIPLSFGQQVIVVA
                     TSLLAAIGAAGVPMAGLVMMSIILSAAGLPLEGIGLILAVDRILDMFRTTVNVWGDTC
                     CAVVIAKSEGEKLKV"
     gene            7763..8641
                     /locus_tag="PKM34_01705"
     CDS             7763..8641
                     /locus_tag="PKM34_01705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ82326.1"
                     /translation="MRKFSIIDEHDPALIHYLDELPLWSAPFALQILDKVIPAKNLRV
                     LDIGFGTGVPLIELASRLGNSCKIYGIDPWKEACDRAGEKIRLFGINNITLFNAPAET
                     LPLGDNSVDLIISNNGLNNVDDMEQVLKESFRVLVPSGKLIFTMNTEETMTEFYSIMQ
                     EVLSEYNLAASVTAMKNHIRQKRKPLPEVAGLLEKSGFIITSVDHDWFHYTFTDGTAL
                     FSHFFIRLAFLGPWLKTVPKNYQQTIFSDIENRINTISSEKGHFRLTIPFVTMTSEKS
                     DNSIISHSCQTFGFPD"
     gene            8859..9395
                     /locus_tag="PKM34_01710"
     CDS             8859..9395
                     /locus_tag="PKM34_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNQ82327.1"
                     /translation="MSQQTNWISDPKHSEILFKVKHMMITNVKGEFRKFHIEADSDGE
                     QFIASSIRVVVDTFSVFTNVDDRDNHLRSADFFDVENYKEMVFNGSSLKKTGDGNFEL
                     TGILNIRGVSKEVKLDVEFGGINKDPWGNEKAGFSISGKINRKEWGLNWNAALEAGGV
                     LVSDEVRISAEVQLVKTS"
     gene            9408..>10101
                     /gene="ygiD"
                     /locus_tag="PKM34_01715"
     CDS             9408..>10101
                     /gene="ygiD"
                     /locus_tag="PKM34_01715"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNQ82328.1"
                     /translation="MDRRSFLQSVSVLGLTGITMKLNALNYASGTFGNTSKMPALFLG
                     HGSPMNAIEENEFVRGFREMGKALPRPNAILCVSAHWETRGTWVTAMDKPRTIHDYGG
                     FPRELYQVRYPAPGNPDLAFESKRLITSADVGLDHQWGLDHGCWSVVKHLYPDADIPV
                     IQLSLDQTKDAGYHYNLAKELSSLRNKGVLIVGSGNMVHNLRMVAWDRLNDVEFGYDW
                     AIEAGTRMKEYIL"
BASE COUNT         2771 a         2255 c         2453 g         2622 t
ORIGIN      
        1 gtgaagatgt cagcaacagc gagtcgggag ccgttccttc caccagggtg aaattcggat
       61 gatggtaaag ggttgagtca taggcaccgg tatgcctgta aacggtgttg tatgtcagat
      121 aatcccattc accgcaggca tactggtcgt gggtggtggc aaagtcgcat ttcagcgtat
      181 gaaacatgag gatcttccga aagctctccc cttcggggaa ttgccaggta ccgcggcggg
      241 ttgtgatcga atcgaatgta aatgtctgaa cgataagggt atcctgcgca gcagaagtaa
      301 ttaccataag cgtcaggatg aaaaccggca ggatctttct catagatgga tttctgaaaa
      361 tatttcccgt aaaagtaata ataacgggaa atgtattttt ccggcggata ttttgaaatc
      421 tttcgaccgt cgggtaaatc cgtcggtgta agattatctc ccgggggaaa atattttata
      481 aaatgataaa cggtgcgaaa ctcttcttaa tattgtaatc acggataatt aaacctccgg
      541 atgattcaat tattaacaaa accaaaaaaa actcatgaaa aaactattat tgattgtggt
      601 tctggtaccg gcactattcc tgacatcgtg tttcaaggaa aaagccgggg agcttggcgg
      661 agaccagtcg ccaatgggtg aagtcggcgt cacggtttcc agttcatcca tggaaattgc
      721 cggtgtcagt aatttcactg ctacggtaac ggccttgaag gacggtgttt catcgtacac
      781 agcaacggcc acggtaacca atccgctgct gaaaaatatt gcggccaatt accccggagt
      841 ggaggttaac ggcgaccagg taagcattac aaacgtgaaa atccagcaga cgaaagaagg
      901 gatcaaaagc atggaaggtc ccggcgccgg cgttctggtc aagtatgatt caaaggtcgg
      961 cgatacctac cctatcgatg gcaccgacag ggagagaaaa gtggtcagca aatcgaccga
     1021 cgatgattat tactacggat tcttcctgat caaaaccatc caggtcgaga tggatcagtc
     1081 gcacgtgaaa agctcaggta tcacaaaata tacatatatc gctaaccaca agtttggttt
     1141 ggttggagtg aaggtagatt ttgacgacca aaccagcgtt acgttcccga tctacacctc
     1201 tgccgaaaat taaacaggta aatccggtct gactgatatt taactgatgg gatcgttttt
     1261 attgccttac cgctggaaaa tcgcaggtat tgtgatggca attgccggca tggccggtgc
     1321 tgccgtttac ggttttttcg attttcgctt taaggtacct gtttttgctg tcgtttccat
     1381 tttcgtagaa ccgaaattct ttaccgtctt ccagaccaat ttttcggaag aggttgtttt
     1441 attttttctg attacaggat taaatctgat catcttttca aaagaaaaag aagaggggga
     1501 agatttggaa aggatacggt tgaaagcatt cgccagggct atgatcacca atgccgcggt
     1561 tcagcttttt gccgttttgt ttgtttatgg cgccggtttt gctgccgtaa tggtattcaa
     1621 cctattgtct gtcaatattt tgtatttgat tttcttttac aggatgagat ggagtctctt
     1681 aaaaaattga gactccatct caaatcaaat ctatttatct atatttgtcc gatttttaaa
     1741 aacgggcttc aatgaacaga atcatcttca tttctttact gctgatcaca gcaatcatcc
     1801 caacatgggc gcaaactccg ggcacattga ccgtgtcggt ttcaaccagc gcggccggag
     1861 ggaattatgc accgaaaaat attgttgcca tctggattga agacaaccag ggtaattttg
     1921 tgaaaacgct gctggcttat gcacagacgc ggaaaaccca tctcaacacc tgggaagcct
     1981 caacaaccgc tgccggcagt ccgtacaaca ccgtcgacgc tattaccggc gccacaaaaa
     2041 ccagccatgc cacccgcacc tgttcctggg atggtaccga tatcaacggg aatattgtat
     2101 ccgatggaac ctacaaatta aggatggagc tgactgataa gaacggcacc gggaatttct
     2161 ccactttcac ctttgaaaaa gatacactcc aggtaaatct aaccccggcc aatgtaccga
     2221 gcttctcgtc aatcgtcatt aactgggaac cggtaattac tgctatcggt gaaccggaaa
     2281 cagccggcag catcactatt tatcccaatc cgtcatcggg tgtcatcaat cttaccggaa
     2341 aatatgatga agtaatagtt ttcgggacat ccggcaggca aatcgtgaaa tccgcaggta
     2401 ataagattga tcttacggca tcacccgatg ggctttattt tgtgcacgtg agaaccggag
     2461 tggaaacaga aattttcaaa gtcgtgaaaa caaatgacct cgaaaaataa accgtcgccc
     2521 tccagcgact ggaaatcacg ccttggtgta gtatattcca ccaatcccga ttattcatac
     2581 gcaaccggtg agagcgctga acccggcacc ctcccccccc aacagcaaaa cctccgggtg
     2641 agtctcgacc ggaagcagcg caaaggcaaa tcggtcaccc tgatccaggg atttatcggc
     2701 agggatgaag acctggagct actggcgaag cagttgaaat cgaaattcgg agtcggagga
     2761 agcgcgaaag agggagagat catcatccag ggcgatttcg tggtaaagac aattgaattc
     2821 ctgcaaaacc ttcattacaa ggtcaaacgg tcgggcggct gaagcataaa taatcatatt
     2881 gaaatgctgc cggtattcag tcaggtttgt caataatatt cttatcttgc attgcctttt
     2941 taccaaaaac atcccgccat gacaaagtct tatctgtatt ttttgatatt gatgattccg
     3001 gccggtcttt ttgcccaggt ttctcccgac tggtcaatga tttataatgg caatgacgat
     3061 tatttcgata acggagtcgc aattgcccta gatccttccg gaaatgtgtt tgtgggagga
     3121 acatcagtat cgatcaccaa tggagcgccc gactatgtcg ttttaaaata cacatctgcc
     3181 ggtgagttca aatggtccta tgaatatgac ggtacgggag cttatgaaga tttcctggcc
     3241 gcaatcacca cagatagtga aggaaatgtt tacgctaccg gtgggagcca tgaagggtcc
     3301 tcaagaaggg attatgtcac cctgaagatt gatggatcag gggcatttgc ctggaaaagt
     3361 atctataaag gactggatgt gaataatgat gatgctgctg cggatatagt tgtggatgag
     3421 aatggatacg tgtatgttac cggtgccagt gccggatatt ccagttcaat gtggacagat
     3481 tttgctacat tgaagttgac tcccggcggc gacacggtct ggacccggcg cctgggagga
     3541 cccggcatgc caaacgaccg ggctacggca atagcagtag ataatttcgg ttatgtctat
     3601 gttacgggtt attttgaagg ggatgtcagc acaagatata attatgcaac ggcaaaatat
     3661 aaacccaatg gtgatacagc atgggtaagg gtttataacg gcaccgggtc tgatgaagac
     3721 agggcttatg ccattgcagt ggatgaaaat aggaatgttt atgtgaccgg ctcaagctgg
     3781 ggtttaaatg ctgatgatta cgcgacagtc aaatacgatt ccaatggtgt tcagaaatgg
     3841 gtcgcccgat acaatgggcc ggccggttca tatgacaaag cttgggattt gaaactcgat
     3901 gatgagggca atgtttatgt aacagggcaa agcggcggaa caagcggtaa ttatgattac
     3961 tgcacgataa ggtataatcc ggatggaggt gaagactggg ttgccaggta taacggtccg
     4021 gataacctgg acgacgtggc cacatcagtt gccatcgatc cttcaggaaa catctttgta
     4081 acaggttatt cttataatcc tccctcgcca tatatcagca caaccgatat ggttctgata
     4141 aaatataatc ccgaagggct ggaagaatgg gtgagccggt tcgacggccc gggcaataat
     4201 tacgaccagg ggtatgccat tgctgccgat ccttccggag atgttttcgt taccggttca
     4261 acagaatatt acggtcaaca aaattccagg gatattttca cggtaaaata cgccggccct
     4321 tctctggtgg aagatttttc ttccggtaat tccattaacc tttatccaaa tccggccgat
     4381 catcaactga ctgttcaggt tccgtgcggg gctgcgctga tttccgtttg cgacctaacg
     4441 ggaaggcaga ttatcgtgca tgaattgaaa gtatcggaaa aagacgaaca aacattcaat
     4501 atttcgggca taccctccgg ggcatatatt gtaaaagtaa cgacgacact gggtatatca
     4561 gaaaagaagc tcatagtata ttagatttga gattatggtg aaaatgacag cggtctacca
     4621 ttcggcagat gaatatatta catgtcagcc ggaacaattc cgggccaacc tggccctgat
     4681 ccggcaaaca atccgggaag ctgttccgga tgcaacggaa gccatcagtt accagatgcc
     4741 tgcattcaaa tttcatggca ttctgtgctt ttatgcagtt ttcaaggatc attacagtct
     4801 ttttgtatct cctgaaattc acaaggcctt tgttgaggag ctgaaaagct ataaaacgac
     4861 caagtcggcc atacactttc catttgaccg gccggttccg gttgaattaa ttgttcaggt
     4921 ggtaaggttt gcggcgatta cgaatctgga aaaggccgaa gccaggttaa agaagcggaa
     4981 gagatgaagg ttattttgag actccatctc ggttaatcca gatggagtct cgcttgattg
     5041 agactccatc tcatgattcg agatggagtc tcaattttcc tacctcaatt attaataatt
     5101 ttaccggcaa aaattgcccg atatgctgac attgcccgat tattcagaca ttattaaagc
     5161 ccagatgagg ctgaaagggg ttgtgaaaaa caccccactg gagccttcca aatcattctc
     5221 cgccatgtcg ggcgcccaca tctacctgaa actggaaaat ttccagtcga cgggctcttt
     5281 taaggtcaga ggggcatata ataaaatcag ttcgttggat cccggggaac tggccaacgg
     5341 cgttgtgtgc gcttcggcag gcaatcatgc acagggcgtt gcttttgccg cacgggctct
     5401 cggtctgaga gctacggttt ttatgccggt gttcacgccg cctctgaaag tgatcgccac
     5461 cagaagctac ggtgctgacg ttaagctgga aggagagaca ttcgacgatg cactgtcagc
     5521 ggcaaagaaa ttccaggaat catcgggcgc tgtctttatc catgcctttg atgatccctt
     5581 gatcattgcc ggccagggga cgatcgggct ggaactgttc caggcgctgg aaaccataca
     5641 ggatgtggtg gttccggtcg gtggtggcgg attgatcgcc ggaattgcca ttgcattgaa
     5701 gaagaccaat cccgatatca ggatcattgg cgtggaagct gagggcgcag catccatgca
     5761 gacatcgctc agggatggaa aattaacccg actaaccagc atgtcgacca ttgccgacgg
     5821 tattgcggtg aaaacgcctg gtgagcttac ttttgcagca gcgaaacaat ttgtagacga
     5881 gattgtgacg gtttcagacg cggagatcgc ccacgcggct tatctgctcc tgcaaagggc
     5941 gaaactgctg gctgagccgg caggggtagc ctcaatggct gctgtccttc ataagaaact
     6001 ggatttcaag aaccgtcagg tcgtgactgt catcagcggg ggaaacatca acatgggttt
     6061 gcttgagcag atccttgaga aggggatgat ggctgaaggc ctcagagcgc ggatcgccgt
     6121 tttgatcccg gatatgcccg gcgaattgaa atcggtgatt accattctgg aaaacctgtt
     6181 tgccaatatt aatgaaatca gccatgagcg ttcgatcact acggttccgg taggccatgt
     6241 gctggtaacc atcaccttta accttcagca gattgagcag ctcgacatgg tttgcaaagc
     6301 gctggagggg aagatgaaga agtttgaggt gctgcgatag ggatgttctg atgagactcc
     6361 atctcaaatt cgagactcca tctcgattgc ccagatggag tctcaatgta tcgagactcc
     6421 atctcaaatt acaaatccca ttctttttgt acatttaatc cctcaaagaa caactccttt
     6481 atgtcgttga aaataaagct ccctgccctt cactggcaaa tcctgatcgc actggcattg
     6541 gcagtcctct tcggaatata tttttccgaa tacgtaaaat atgtcagctg gatgggaacg
     6601 atctttctca gggccctgca aatgatcatt atgccgctgg taatcagctc gatgatctgc
     6661 ggagtggcta acataggcga cgcccagaac ctgggccgga ttggattaaa gacaatgctg
     6721 ttttatgtca tcacaacagt gatagctatc ttaaccgggc ttgtgctggt taatttcttt
     6781 cagccgggtg tcggcgccga ccttaacctg atcgaaaaag cggagatccc tgcagcctcc
     6841 ggttcattag ggcaaatcct gatcgatatc gtaccggaaa atgtcttcgc cgtcatgtcg
     6901 gtcaaccggc aattgctttc ggtaatcctg ttcgccctgc tttttggatt ttttatcatc
     6961 aaagtatccg aaaaaccaag aaaacagctt accgactttt tcaatgccgt gtttgaagtg
     7021 atgatgaaga tcactttttt cattattaaa ttcacccctc tgggaatttt cgggctggtt
     7081 gcagaaaaga tcgccccgca caaagatgtc gcagcgcttt ttgaaagcct cggaatatat
     7141 atggtagttg ttttattggg gttattcatt catgccgttg tatttatgcc gttgatcatc
     7201 cgatttatcg gccgtattaa ccctctccgg catttcaatg ccgtaaaaac ggctctgttc
     7261 actgctttct caacatcttc ctcaaatgcc acacttccac tgacaatgga atcgattgaa
     7321 gtgaatgccg gggtgtccaa taaaatcacg agtttcactc ttccattggg ggcaaccata
     7381 aatatgaacg gaactgcact ttacgaatgt gttgcggtca tcttcatcgc ccaggcttac
     7441 ggcatcccgc tcagcttcgg acaacaggtt attgtcgttg ctacttcgct gctggctgcc
     7501 attggtgcag ccggtgtgcc catggcaggg ctggtgatga tgtcgatcat attatcagct
     7561 gccggtctgc cacttgaggg aatcgggttg atcctggccg tcgaccggat actcgacatg
     7621 ttccgcacca ccgtcaatgt gtggggcgac acttgctgcg cggttgttat tgctaaaagt
     7681 gaaggagaaa agttaaaggt ataatataag actccatccc gatgggtgag atggagtctc
     7741 gaaataaatg agatggagtc tcatgagaaa attttctatc atcgacgaac atgaccctgc
     7801 cctgatccat tacctggatg aactgccgct ttggtctgca ccgtttgccc tgcaaatcct
     7861 cgataaagtg atcccggcta aaaacctccg cgttctcgat atcgggttcg gcacaggggt
     7921 accgctgatc gaactggctt cccgcctggg caattcatgt aaaatctacg gcatcgatcc
     7981 gtggaaagag gcgtgcgaca gagccggcga gaaaatcaga ctgttcggga tcaataacat
     8041 caccctgttc aatgcgccgg cagaaaccct tcccctgggc gataactctg tcgatcttat
     8101 catcagcaat aacgggctga ataatgtcga tgatatggaa caggtgctga aagagtcttt
     8161 ccgtgtcctt gtaccgagcg gtaaactcat ctttaccatg aataccgaag aaactatgac
     8221 ggagttttac tcaatcatgc aggaagtctt atcggaatat aatctggcag cttcggtcac
     8281 agccatgaaa aatcatatcc gccaaaaaag aaaacctttg cccgaagttg ccggcctgct
     8341 tgagaaatcg ggattcataa taacatcagt tgaccacgac tggttccatt ataccttcac
     8401 cgacggcact gcccttttta gccatttctt catccgactc gcttttctcg gaccctggct
     8461 caaaaccgtt ccaaaaaatt atcagcagac catattcagc gacatagaaa acaggataaa
     8521 cacaatctcg tccgaaaaag gacattttcg tctgactata cctttcgtaa ccatgacttc
     8581 ggaaaaatca gataactcca ttatttcaca ttcgtgccaa acttttggtt ttcccgatta
     8641 aaaaatcatt ttccgtgtga acaatccgtc aagattttca ttatagttta aagcacaaaa
     8701 tcttataaaa accggtcatc tttataatgg tttttcatga attttcgtgt ttcctgattt
     8761 tgtggctctg aaagccctta aaagaaattt acattaagaa ttaaaaatta agaattaaaa
     8821 attaagaatt aaaaattaag aattaaaaat tatatctgat gtcacaacaa acaaactgga
     8881 tctctgatcc caagcacagc gaaattttgt tcaaagtcaa acatatgatg atcaccaacg
     8941 ttaagggtga attcaggaaa tttcatatcg aagccgattc cgacggagaa cagttcatcg
     9001 cttcttcaat tcgtgtagtg gtggatacct tctcagtctt cacgaacgtc gacgatcgtg
     9061 acaaccatct gagaagcgcc gactttttcg acgtcgaaaa ctataaggaa atggttttca
     9121 atggttcttc tttgaagaaa accggggatg gcaattttga gctgaccgga atcctgaata
     9181 tcaggggagt aagcaaagag gtgaaacttg atgttgaatt cggtggtatc aacaaagacc
     9241 cctggggtaa tgaaaaagca ggtttttcta tcagcggcaa gataaaccgc aaagagtggg
     9301 gcctcaactg gaatgcagcc ctcgaagccg gaggagtgct ggtcagcgat gaggtgagga
     9361 tcagcgccga ggtccagctg gttaaaactt cgtgaaaaat tcaggatatg gaccgcagat
     9421 catttcttca gtctgtttca gtgttagggc tgacggggat cactatgaag cttaatgcat
     9481 tgaactatgc cagcggcact tttggcaaca catcaaagat gccggcgctc ttcctgggtc
     9541 acggaagccc tatgaatgcc attgaagaga atgaatttgt gagaggtttc agggaaatgg
     9601 ggaaagccct gcccaggccg aatgccatac tctgcgtttc cgcccactgg gaaacccgcg
     9661 gcacatgggt tacggccatg gacaagccga ggaccatcca tgattacggg ggattccccc
     9721 gtgagcttta ccaggtgcgg taccccgcac cgggaaatcc tgatctggcg tttgaatcta
     9781 aaagattgat cacctctgcc gatgtaggcc tggatcatca atggggcctt gaccacggtt
     9841 gctggagtgt tgtaaaacac ctttacccgg atgcggatat cccggttatc cagttaagcc
     9901 ttgatcaaac taaagatgca ggatatcatt acaatctggc aaaagaacta tcatcgctca
     9961 gaaacaaagg agtcctgatc gtcggcagcg gcaatatggt acataacctt cggatggtgg
    10021 catgggacag gctgaatgat gtggaattcg gctacgactg ggcgattgaa gccggtaccc
    10081 ggatgaaaga atatattctg t
//
LOCUS       DAOQXS010000030        10033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7196_length_10033_cov_3.126478, whole genome shotgun sequence.
ACCESSION   DAOQXS010000030 DAOQXS010000000
VERSION     DAOQXS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7196_length_10033_cov_3.126478"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>280)
                     /locus_tag="PKM34_01720"
     CDS             complement(<1..>280)
                     /locus_tag="PKM34_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82329.1"
                     /translation="DSDTNQFGFTFFSGNRTINTGNFYFQPEWIDGRRQTGDTLKVSG
                     SEEARFAFRLYTSSDSIINKDQYIEYLFTLKGDNYMLGFDVNFRNLTQT"
     assembly_gap    281..290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<291..700)
                     /locus_tag="PKM34_01725"
     CDS             complement(<291..700)
                     /locus_tag="PKM34_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82330.1"
                     /translation="MNNSRNTIIGFLLIFAILIGYYFLTAPSKEELAEQRRRQDSVMA
                     LQRQKDSIALARQILADQQKATEPAQEAVTDQITEPAKQLTGLVDRYGAFALASQGEN
                     KKYFLENDLVRIGLSSKGGRISYVELKNFKTWDG"
     gene            complement(767..2398)
                     /locus_tag="PKM34_01730"
     CDS             complement(767..2398)
                     /locus_tag="PKM34_01730"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ82331.1"
                     /translation="MPDTKYIFVTGGVSSSLGKGIISASLAKLLQARGFSVTIQKLDP
                     YINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVPTSQANNVTTGRIYKTVIE
                     KERRGDYLGETVQVIPHITDEIKRNIQILGNKYGYDFVITEIGGTVGDIESLPYIEAV
                     RQLKWEMGGHCLVVHLTLVPFLAAAGELKTKPTQHSVKTLLESGVQPDIIVCRTEKHL
                     NQNIRNKIALFCNVESTSVIESIDARTIYEVPLLMREEKLDAEVLRKLGIRPTQPPEM
                     GKWEEFIYRIRNPKYDVRICLVGKYVELKDAYKSIQESFIHAGAANYCKVHVQTIQSE
                     NLNETNAAEKLKGFDGILVAPGFGSRGIEGKIEAIRFARENNIPFLGICLGMQCAVIE
                     FARHVLGLEKAHSTEMDPDTPHPVIDMMEEQKTIQEKGGTMRLGVYDCRILPDTKVSE
                     IYPDTDFKERHRHRFEFNNRYLNDFEKNGMIAAGINPQRNLVEIMELRDHPWFIGVQF
                     HPEYRSTVAQPHPLFVKFIEAAVRQKRLRHPEEHS"
     gene            complement(2421..3905)
                     /locus_tag="PKM34_01735"
     CDS             complement(2421..3905)
                     /locus_tag="PKM34_01735"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ82332.1"
                     /translation="MSESSQSKKKEQFHEYVNSLENWITDEKSGFEFVNLVGQLYYDK
                     SIELIIFRSQLIDRSSSVILKKHQHSLSVLGKELKIQVSVMLAKELLSMDIPPARIDI
                     GRLNKEWTEEGKNYLSPRAFLQDKLKNLLGKKAGYDKPVDVIVYGFGRIGRLVTRELI
                     IQGNGTQLNVKAVVTRGNSPEEITKRASLFKNDSIHGPFRGVAIEDHENKLLLINGHK
                     VKMLSANNPEDIDYEAEGIHNALLIDSTGVWTKREDLARHLKAKGVSKVMLTAPAKGD
                     VPNIVYGVNHDDGTVDYKTETIYSAASCTTNAIVPILYVIENRLGIDKGHIETIHAYT
                     NDQNLTDNYHKKPRRGRSAPMNMVLTSTGAASAAAKAIPSLKGKLTANAVRVPIPNVS
                     LAILSLTVQKQTTQQEVVEILRDAALNGRLVEQIRFSASTEAVSTDFIGDSCAGIFDA
                     PATLVSADGKSIILYVWYDNEFGYTIQALRLAKHIAGVQWETYY"
     gene            complement(4030..4896)
                     /locus_tag="PKM34_01740"
     CDS             complement(4030..4896)
                     /locus_tag="PKM34_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNQ82333.1"
                     /translation="MRQLKISKSITNRETASLDKYLQDIGREELITAEEEVELARRIR
                     SGDDKALDKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRLVRLPLNQVGSLNKIKKATSRLEQEYERLPSVEEIAK
                     KLDIPEHKLDKAMRITTRYVSMDAPIAEDEDTKFLDVFVSDDTPRTDTNLMRESLNKE
                     IQRSLSTLTDKERDVINLYYGIGMNHGLTLDEIGAKFNLTRERVRQIKEKAIRRLKHT
                     SRSKLLKAYLGE"
     gene            complement(5016..6479)
                     /locus_tag="PKM34_01745"
     CDS             complement(5016..6479)
                     /locus_tag="PKM34_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ82334.1"
                     /translation="MKRLIQILSVITLSVLTIWGIKGIFSDNDKEDLVKEMGFKTEGD
                     ARVLPANYLPVAPFSGPDFTTAAEKTVHGVVHIKSEFSVRTGGYDDFFAPFRDFFGYP
                     YRGDNTYTGFGSGVIISNDGYIVTNNHVVEGAGRVNVTLNNKREYEAEIIGTDPSTDL
                     ALLKIDGSDIPFITIGNSDNTKIGEWVLAVGNPFNLTSTVTAGIISAKARNINILGTP
                     GAIETFIQTDAAVNMGNSGGALVNMNGELVGINAAIASNTGSYAGYSFAIPANIVRKV
                     VDDLLNYGLVQRAYLGVSIREVNSKLVKEKQLATDNGVFIESVTETGGAKEAGIRQGD
                     IIVSVNAHPVRTNAELLEVIGQHNPGDIVSVLVNRDGTDKEFRVELRNQEGSTSVEKK
                     GEDFYLSDLGATLEQVPDDEKAKLSITGGLKVVELQDGMLKKGGVQKGFIILKINGIK
                     ISSRQDANYALANLRSGVIRIEGVYPNGMKMNYGFIL"
     gene            6644..7447
                     /gene="dapF"
                     /locus_tag="PKM34_01750"
     CDS             6644..7447
                     /gene="dapF"
                     /locus_tag="PKM34_01750"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNQ82335.1"
                     /translation="MKIPFYKFHGTGNDFILINNRENEFVAREDLISRMCHRRFGIGA
                     DGLILLEKAPGYDFGMHYYNSDGKESTMCGNGGRCIVALADFLSLTGNDCRFKAIDGP
                     HRGKVLHREGNNWQVSLQMADVAIIENMNDEFVLNTGSPHLVTFADNVSTVDVDGEGR
                     AIRNREVFKSDGINVNFAEIRDDGLFVRTYERGVEAETLSCGTGVTASAIAFAIKSGC
                     FNEKVKVSTPGGVLAVRFEYNKLGHFSSVLLEGPATLVFVGETDTNYYL"
     gene            7467..8012
                     /locus_tag="PKM34_01755"
     CDS             7467..8012
                     /locus_tag="PKM34_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ82336.1"
                     /translation="MKPISGGIVSLRALEPEDADLLYEWENDCRIWHLSNTIAPLSRF
                     ALEQYILNTGQDIFATRQLRLMIDLQSKEGKTTIGSVDLFEFEPLHLRAGAGILVNEK
                     YRGRGYASEALTLLIGYAFGTLRLHQLYANVSASNHDSIKLFEKNGFLPAGVKKDWNR
                     LPNKWEDELMFQLINPSRVIR"
     gene            7999..9087
                     /gene="mltG"
                     /locus_tag="PKM34_01760"
     CDS             7999..9087
                     /gene="mltG"
                     /locus_tag="PKM34_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNQ82337.1"
                     /translation="MLSDKMAVQIRKPEPRKKRGKFHRIILLILAGAVVISLVAAWSL
                     FNMILKPNVTVPGGKASLYIPSGSTYQDVKGILKSNAWLRNSSSFEWMAQRKNYPARV
                     KPGHYIIHNGMSNNSLVNMLRSGVQTPVDVTFNNILNKEELAGKIAGQIEADSLSLIE
                     CWNDKEFLKSLNTNPAKVIMLFLPDTYELWWTTSAREFTQRMAKESDRFWNEERLKKG
                     RDARMSREEVIILASIIEKETQMNDEKPVMAGVYINRLKKGWPLQADPTVKFALGRLD
                     LKRVLKIHTEFDSPYNTYKYTGLPPGPICIPSKASIEAVLNFRKHDYMFFCAKADMSG
                     YHVFSRTLAEHNRYAAAYQAELNRRRIR"
     gene            9152..>10033
                     /locus_tag="PKM34_01765"
     CDS             9152..>10033
                     /locus_tag="PKM34_01765"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNQ82338.1"
                     /translation="MLFATVTISQPIDYHDASISDSTNPAEPDKKRLTGVSATAGGLY
                     AGTMTGLYYLWYADYPLSKFHFINDNNHWLWMDKIGHVTTSYWIGKIGYESLRWSGLE
                     EKKAVWYGGTWGLVYLTTVEIFDGFSREWGASDGDFAANVMGTAAFIGQQLLFEDQIF
                     VFKYSFHPTKYADYRPDLLGENMAQSLLKDYNGQTYWLSANLSAFLPSESRFPRWLAF
                     SFGYSAEGMLGAKSNPDEYNGKPLPHFGRYNQYFITLDLDLTKIRTRSETLRFLLNLV
                     GYVKVPFPAVEFNKKDHV"
BASE COUNT         2516 a         2299 c         2353 g         2855 t
ORIGIN      
        1 cgtctgggtg aggttgcgga aattcacatc aaagccgagc atgtagttat cgcctttgag
       61 ggtgaacaag tactcaatat actgatcttt attaatgatg gagtcggagg atgtataaag
      121 cctgaaggca aaccgggcct cttcacttcc gctgacttta agcgtatctc ctgtctgtcg
      181 ccggccatca atccattcgg gctgaaaata gaaattaccg gtattgattg tgcggttacc
      241 gctaaagaag gtaaatccga actggttggt gtcggaatcc nnnnnnnnnn tgtccgtccc
      301 aggttttgaa gtttttgagt tcaacataag aaatccggcc tcctttggaa gaaagtccaa
      361 tcctgaccag gtcattttcc agaaaatact ttttgttctc accttgtgaa gccagtgcaa
      421 aagccccata gcggtcaacc aggcctgtca attgcttcgc cggttctgta atttggtctg
      481 tgacagcttc ctgtgcgggt tccgttgctt tttgctgatc ggccagaatc tgccgtgcca
      541 gggcaatgga atctttttgc cgctggaggg ccatgaccga gtcctgccgg cgtctttgtt
      601 ctgctaattc ctctttggat ggtgcggtaa gaaaataata tccgatcagg atggcaaaaa
      661 tgagtagaaa tcctatgatc gtatttctgg aattattcat acaattttcc tggtttttat
      721 ttagtggggc aaagataaat caattgcctc aaagggatta aaaaaactat gaatgctctt
      781 ccggatgacg cagtcgtttt tgacggacag ccgcttcgat gaatttcacg aataaagggt
      841 gaggctgtgc tacagtgctc cggtactccg gatgaaactg tacgccaata aaccatggat
      901 gatctcttag ctccataatc tccaccaggt ttctctgggg attgataccg gcagcgatca
      961 tgccgttctt ctcgaagtca ttgagatagc ggttattaaa ttcaaagcgg tgcctgtgcc
     1021 tctctttgaa atcagtatcc ggataaattt cactgacttt tgtatcgggc aagatacggc
     1081 agtcgtaaac acccaatcgc atggtgccgc ctttttcctg gatggtcttc tgttcctcca
     1141 tcatgtcgat gaccggatga ggcgtatccg ggtccatttc cgttgaatgg gctttttcca
     1201 ggcctaggac gtgcctggca aattcaataa cggcacactg cattcccagg cagataccca
     1261 ggaaagggat attgttttcg cgtgcaaaac ggatcgcttc tatctttcct tctattcccc
     1321 gggaaccaaa tcccggcgct accagtatcc cgtcgaatcc tttcagtttt tcagccgcat
     1381 tggtttcatt caggttttcg gattggattg tctggacatg cactttgcag taatttgccg
     1441 ccccggcatg gataaacgat tcctggatcg atttgtaggc atctttcagt tcgacgtatt
     1501 taccgacaag gcagattctg acatcgtact ttgggttcct gatacggtaa atgaactcct
     1561 cccacttccc catctcgggc ggttgtgttg gccgtattcc cagctttcgc agcacttccg
     1621 catccagttt ttcttcccgc atcagaagcg gcacctcata gatcgtcctg gcgtcaatgg
     1681 attcgatgac cgaagtagac tcgacattac aaaacagggc aattttattc cggatgttct
     1741 ggttcagatg tttttcggtc cggcacacga taatatccgg ctgtacaccg gattcgagca
     1801 gcgtcttgac agagtgctgg gtcggttttg tcttgagttc gccggcagca gccagaaaag
     1861 ggaccagggt gaggtgaacg accaggcaat ggccacccat ttcccatttc agctgcctga
     1921 cggcttcgat ataaggcaat gattcgatat cgccgactgt tccaccgatc tctgtgatca
     1981 caaaatcata tccgtattta ttgcccagga tctggatatt ccgcttaatt tcgtctgtaa
     2041 tatgcgggat cacctggacg gtttctccga ggtaatcgcc tctccgctct ttttcgatga
     2101 cggttttata aatcctgccg gttgttacat tgttggcctg ggatgtgggc acattcagaa
     2161 atcgctcata gtggcccagg tccaggtcgg tttccgcacc atcttcggta acataacact
     2221 ccccgtgttc atagggatta agggtgccag ggtcaacatt aatgtaagga tccagtttct
     2281 gaatagtgac tgagaacccc ctggcttgca gcagttttgc cagcgaagcc gagatgatcc
     2341 ccttgccaag ggatgatgaa acgccaccag taacgaatat atattttgta tccggcatgt
     2401 tatttatatg atctaaaatt ttaatagtag gtttcccact gcactccggc aatatgttta
     2461 gccagacgga gtgcctggat cgtgtagcca aactcgttgt cataccaaac gtacaggatg
     2521 atcgatttcc cgtcggctga aaccagcgta gcgggggcat caaaaatccc ggcgcagcta
     2581 tctccgataa aatcagttga cacggcttcg gttgatgctg agaaccggat ttgttcgacc
     2641 agtcttccgt tcagcgcggc atcacgaagt atctccacca cttcctgttg tgtggtttgc
     2701 ttttgcactg tcaatgagag aattgccagt gaaacgttag ggatcgggac cctgaccgcg
     2761 ttggcggtca gctttccttt cagcgaaggg atggctttgg cggcagcaga agcggcgccc
     2821 gtagaggtaa gcaccatgtt cattggagcg gaacggcctc ttctcggttt tttatggtag
     2881 ttatcggtca ggttctggtc gttggtgtaa gcgtggattg tttcaatatg gcctttatca
     2941 atgcccagtc tgttctcgat cacgtaaaga atgggcacta tagcattggt ggtgcaggat
     3001 gctgctgaat agatcgtttc tgtcttataa tccaccgtgc cgtcatcgtg attcacaccg
     3061 taaacgatat tcggtacgtc tcctttagcg ggcgccgtca gcatcacttt actcacccct
     3121 ttggccttca ggtgccgggc aaggtcttca cgcttggtcc acacaccggt actgtcgatc
     3181 agcagtgcat tatgaatccc ttctgcctca taatcaatat cttccggatt attagctgaa
     3241 agcatcttca ctttatgacc attgatcagc agcagtttat tttcatggtc ttcaatggct
     3301 acgccacgga aagggccatg gattgaatca tttttaaaca aggaggcacg ttttgtgatc
     3361 tcttcgggtg aattgcctct tgtgacgaca gctttgacat tcagctgcgt cccgttcccc
     3421 tggatgatca gctcccgggt aaccagacgt ccgatacggc cgaagccgta tacgatcaca
     3481 tccaccggtt tatcgtaacc ggcttttttg cctaaaagat ttttcagttt atcctgcaaa
     3541 aatgccctgg gtgaaagata atttttcccc tcttccgtcc attctttatt caaccggccg
     3601 atatcgatcc gggcaggcgg tatatccatg cttaacagct ctttggccaa catgaccgaa
     3661 acctggatct taagttcttt tccaagaacg gaaagcgagt gttgatgttt tttcagaatc
     3721 acactggagg agcggtcaat caactggctt ctgaaaatga tcagttcaat ggatttatca
     3781 taataaagct gaccgacaag gttaacgaat tcaaaacccg acttttcgtc tgttatccag
     3841 ttctccaatg aattgacata ttcatggaac tgttccttct tctttgattg gcttgattct
     3901 gacatagtta tgtggtttta tccgtttgta attggttaaa gatcatgttg caataatagc
     3961 aaaaataaaa aagcaaaagg ccatagttcg taaatgatct atggcctgaa aaagtccttt
     4021 ataaaaccgc tattctccga ggtaggcttt cagcagttta ctgcgcgaag tatgtttcag
     4081 tcttctgatc gccttttctt taatttgcct gaccctttca cgggtgaggt tgaactttgc
     4141 accgatctcg tcgagggtca gaccgtggtt cataccaata ccgtaataaa ggttgatcac
     4201 gtctctttct ttgtcggtca gggttgaaag cgagcgctgg atttctttgt tgagcgattc
     4261 ccgcatcagg tttgtatcgg ttcgtggcgt atcgtccgaa acaaatacat ccaggaattt
     4321 ggtgtcttca tcttctgcga tcggtgcgtc catcgacaca tagcgtgtgg tgatccgcat
     4381 ggctttatcc agtttatgct cgggaatatc gagttttttg gcaatttctt caacagatgg
     4441 gagccgttca tattcctgtt ccagacgcga agtagctttc ttaatcttat tcagcgatcc
     4501 gacctgattg aggggtaacc tgaccagcct ggattgttca gccagtgcct ggaggatcga
     4561 ttgccggatc caccagacgg cataggaaat aaatttaaat ccgcgagtct catcgaatct
     4621 ctgtgcggct ttaatcaacc cgagattacc ttcattgatc aggtccggta ggctaagccc
     4681 ctggttctga tattgtttgg ctacggaaac aacaaacctg agattagctt ttaccagctt
     4741 atccagggct ttgtcgtcac ctgacctgat ccgccgggcc agttcaactt cttcttcggc
     4801 tgtaatcaat tcctcccgtc cgatgtcctg cagatactta tcaagtgaag cggtttcgcg
     4861 gttggtaatt gattttgata tcttaagttg tctcatgaat ctcttctaaa atcaaaaatt
     4921 gtaatcctta aatatacgga taaaatccgt ctttgtcaag aaaaacgtgc aaagatacaa
     4981 attgttttgg gctatttaga atttttttaa atagattaaa ggataaaccc atagttcatc
     5041 ttcattccat tgggatagac gccttctatt ctgatcacgc cacttcgcag gtttgccagt
     5101 gcataattgg cgtcctggcg cgaactgatt ttaattccgt taatcttcaa gatgataaaa
     5161 cctttctgta ctccgccttt tttgagcata ccatcctgca attctacaac tttcaagcct
     5221 ccggtaatac tcaatttggc tttttcgtca tcgggaacct gttcaagggt tgcccccagg
     5281 tcggaaaggt agaagtcctc ccctttcttt tccaccgatg tactgccttc ctggtttctc
     5341 agttccacgc ggaattcctt gtctgttcca tccctgttga ccaggacgct gacaatatcc
     5401 cccgggttgt gctggccgat aacttcaagc aactcggcat ttgttctgac gggatgggca
     5461 ttgaccgaga cgataatatc gccctgccgg atcccggctt cttttgcgcc gccggtttcg
     5521 gtaacggatt cgatgaaaac gccgttatcg gtagccagtt gtttttcttt gaccagcttg
     5581 gaattcactt cacggattga cactcccagg taagcccgtt gcacgagccc gtaattcagc
     5641 aggtcatcga ctactttcct gacgatattg gctggtatgg caaaggaata gcctgcgtag
     5701 gatccggtat tgcttgcaat ggccgcattg atcccgacca gttcgccgtt catattgacc
     5761 agggctccgc cgctattacc catattaacg gctgcatctg tctgaataaa tgtctcgatt
     5821 gctcccggtg tgcccagaat gttgatattc ctagccttgg cgctgataat acccgccgta
     5881 accgtggatg taaggttgaa cgggtttccc acagccagta cccactctcc tattttcgta
     5941 ttgtcagaat taccgatagt aataaatgga atatcgctgc cgtcaatctt gaggagtgca
     6001 aggtccgtag aaggatctgt acctataatt tctgcctcgt attccctttt gttgttcagg
     6061 gttacattca ctcttcctgc tccttccacc acatggttgt tggttacgat atagccatca
     6121 ttggaaatta tgacacctga accgaatcct gtataggtat tatctcctcg atacgggtaa
     6181 ccgaaaaagt cgcggaacgg ggcaaaaaaa tcgtcataac ctcccgtcct gacactgaac
     6241 tccgatttta tatgaacgac gccgtgtaca gttttttcag cagcagtagt aaagtcaggc
     6301 cctgagaaag gtgcgacggg caggtaatta gcaggtaaca ctctggcatc accttccgtt
     6361 ttaaaaccca tttctttcac caaatcctct ttatcattat ccgaaaagat accctttatt
     6421 ccccaaatgg taagtacgga gagggtaata accgataata tctgaattaa ccttttcatt
     6481 gttcaatttt ttgatttgct attataataa tttcgggaaa aacgccatga aattcgcaaa
     6541 tagtgtgcct ttccactgtt aaattatgtt aaaagaaagg caaagcaata ttgctcttta
     6601 actccatttt tctctacttt tgaatcttaa attcagaaaa ccattgaaaa ttccatttta
     6661 taagttccac ggaacaggca atgatttcat cctgattaac aaccgggaga atgaatttgt
     6721 tgcaagggaa gatttaatat cccggatgtg ccaccggcgt tttggaatcg gagccgatgg
     6781 gcttatactg ctggaaaaag ctcccggtta tgattttggc atgcattatt acaacagcga
     6841 tggaaaggaa agtacgatgt gcggaaatgg cggccggtgc atagtggccc tggctgactt
     6901 tttgtcactc accgggaatg attgccggtt taaagcgatt gacgggcctc accgcggtaa
     6961 agttctgcac cgcgaaggaa ataactggca ggtgagctta caaatggcag atgtagccat
     7021 catagaaaac atgaacgacg aattcgttct caataccggc tctccgcacc tggtgacatt
     7081 tgccgacaat gtcagcaccg tggatgttga cggtgaaggc agggcgatca ggaaccgcga
     7141 agtttttaag tccgatggga ttaatgtgaa ttttgcagaa atccgggatg acggattatt
     7201 tgtgcggact tatgagcggg gtgtcgaagc ggaaaccctt tcctgtggca ccggggtcac
     7261 ggcatctgcc attgcttttg ccataaaaag cgggtgcttt aacgaaaagg tcaaagtttc
     7321 gactcccgga ggtgtactgg ctgtcaggtt tgaatataat aagctgggcc atttttcgtc
     7381 tgttttactg gaagggccgg caacactggt ttttgtggga gaaacagaca ccaattatta
     7441 tttataagat gatgaccgaa tcactcatga aaccgatttc cggcgggatt gtttccctcc
     7501 gtgcgcttga gccggaggat gccgatctcc tttatgaatg ggagaatgat tgcaggatat
     7561 ggcatctttc caacaccatt gcccctttat cgcgatttgc cctggaacag tacattctga
     7621 ataccggtca ggatatcttc gctacacgtc agctccgcct gatgattgac ctgcagagca
     7681 aagagggaaa aacaactatc ggatcggtcg acttgtttga atttgagccg ttgcatctcc
     7741 gtgcaggtgc aggtattctt gtcaatgaaa aatacagggg cagagggtat gcttcagaag
     7801 cccttacttt gctgatcggg tatgctttcg ggaccttgcg cctgcaccag ctatacgcca
     7861 atgtttcggc atcgaatcac gacagcatta aactatttga gaaaaacggg ttccttcccg
     7921 ctggtgttaa aaaggactgg aacaggcttc caaataaatg ggaagatgaa ttgatgttcc
     7981 agttgatcaa tccatcacgt gttatccgat aaaatggctg ttcagatcag aaaaccggaa
     8041 ccccgaaaaa agagagggaa gtttcaccga attatcctgc tgatccttgc aggcgcggtg
     8101 gtaatctccc ttgttgcagc ctggtcgttg tttaatatga ttttaaaacc gaatgttaca
     8161 gtccccggcg ggaaagcttc tttatacata ccatccggct cgacctacca ggatgtgaaa
     8221 ggaatactga aaagtaatgc gtggttgaga aacagctctt cgttcgaatg gatggctcag
     8281 cgaaaaaact acccggctcg tgtcaagcca ggtcattaca tcatccataa cggtatgagc
     8341 aacaatagcc tggtgaatat gctcagatcc ggagtgcaga cgcctgtcga tgttacattc
     8401 aacaatatcc tgaataagga ggaactggcc ggaaaaattg ccggtcaaat tgaagcagat
     8461 tcattgtcac tgatcgaatg ctggaatgac aaggaatttt tgaaatcgct gaatacgaat
     8521 cccgcaaagg tgattatgct ttttctgcct gacacctacg agttgtggtg gacgaccagc
     8581 gcccgggaat tcacgcagag gatggcaaaa gagtcggacc ggttctggaa tgaggaacgg
     8641 ttgaagaaag gcagggatgc ccgtatgagc cgggaagagg tgatcatcct tgcctccatc
     8701 atcgaaaaag agacccagat gaacgatgaa aaacctgtta tggctggtgt ttacatcaac
     8761 cggctgaaaa aaggctggcc gctgcaagct gatccgacag ttaaattcgc actgggccgg
     8821 cttgacctga aaagggtact gaaaattcat actgaattcg attcccctta caatacgtat
     8881 aaatataccg gattaccgcc cgggccgatc tgtataccct caaaagcttc tatcgaagcg
     8941 gtattgaatt tcaggaaaca tgattacatg tttttttgtg caaaagcgga catgtcggga
     9001 tatcatgtct tctccaggac cctggccgag cataatcgat atgccgcggc ttatcaggca
     9061 gaattgaaca ggaggagaat cagatgaaat gttgattgtt ctaaaaaata aggtgctaaa
     9121 gggcaaaata atattgcatg gattgttttt catgctgttt gccacagtga caatttccca
     9181 accgattgat tatcatgacg ccagtatttc agattccacg aatccggctg aacctgataa
     9241 aaaaagactg accggcgttt cagccactgc cggagggcta tacgcaggta ccatgaccgg
     9301 attatattat ttatggtatg ccgattaccc gctctctaaa tttcatttta ttaacgacaa
     9361 taaccattgg ttgtggatgg ataagatcgg gcatgtcaca acttcttact ggattggaaa
     9421 gattggatat gaaagtctta gatggagcgg attggaggaa aagaaagcgg tatggtacgg
     9481 ggggacatgg ggattggtat acctgactac tgtcgaaatc ttcgatgggt tttcacggga
     9541 atggggcgcc tccgatggtg attttgcagc gaatgtgatg ggtactgctg catttatcgg
     9601 tcagcaatta ttatttgaag atcagatttt tgtgtttaaa tactcatttc atcccacaaa
     9661 atatgcagac tatcggcctg atctgctcgg ggagaatatg gcgcagagcc ttttaaagga
     9721 ttataacgga cagacctact ggttatcagc caatttgtct gctttcctcc cgtcagagag
     9781 ccggtttccc cgatggttgg cgttctcttt cggttacagc gcagaaggga tgctcggggc
     9841 caaatccaat ccggatgaat acaacgggaa acccctaccc catttcggca ggtataacca
     9901 gtattttata acacttgact tggacctgac aaagatcagg acacgttccg aaacattacg
     9961 atttttactc aacctggtcg ggtatgtgaa agttccgttt cctgctgttg aattcaataa
    10021 gaaagaccac gta
//
LOCUS       DAOQXS010000031         9733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7470_length_9733_cov_3.238686, whole genome shotgun sequence.
ACCESSION   DAOQXS010000031 DAOQXS010000000
VERSION     DAOQXS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7470_length_9733_cov_3.238686"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKM34_01770"
     CDS             complement(<1..1039)
                     /locus_tag="PKM34_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82339.1"
                     /translation="MRNFSKYLLTMMILLISSSVVLAQLRVTNSGGKSPVLPESRAVL
                     YQQTNSSGAGMASQDFETVYDAYDCQGADDFYVPAGMQWTIQTITATGSGSTTATLVH
                     VYIYADNAGMPAASASTTLMNLTCTNANAVLTINIPGNIVLGPGHYWVSVQDASPYAT
                     YGQWYWTRTTSIYNSGACWRNPGNGFGLGATTWTYMANIGYPNNDFMFRLEGTSGPVP
                     TCDYRIDLYDHYGDGWNGGYLDVLVNGAVVLDNITLASGSGPAIYYFTVNSGAPITTI
                     FYAGGWAYECYYYIFNYMGAQVWVSDGYGSYVPPPNILPGQLYGNCPAQGAVEGYVFN
                     YDGLAIAGATLT"
     gene            1433..2341
                     /locus_tag="PKM34_01775"
     CDS             1433..2341
                     /locus_tag="PKM34_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ82340.1"
                     /translation="MRAEKSLTNSSLFPWIILVILAVIWGSSFILIKKGLNYFTSVEV
                     GALRISIAFLFLLPFVFKRIRRLSRREWKFLILNGMIGSGFPAFLFAKAQTGIDSSLA
                     GILNSLTPLFTLIVSLSFFSLKIRWFNILGVIIGLIGAIGLISISGGKSFDFNFGYAV
                     YIIFATICYATNVNIVKYKLKDTDALTITVFSFFTIGIPILLYLLLFTGFINKMTTIP
                     TSWEGLGYIGILAVVGTGMALIAFNKLVKIASPVFAASVTYLIPVVAVSWGMIDGEKI
                     TILAFFWMMVILLGVFLVNKKKAVKL"
     gene            2426..2731
                     /gene="rplU"
                     /locus_tag="PKM34_01780"
                     /pseudo
     CDS             2426..2731
                     /gene="rplU"
                     /locus_tag="PKM34_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            2852..3139
                     /gene="rpmA"
                     /locus_tag="PKM34_01785"
     CDS             2852..3139
                     /gene="rpmA"
                     /locus_tag="PKM34_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNQ82341.1"
                     /translation="MAHKKGAGSSSNGRESHSKRLGVKIYGGQFAKAGNIIVRQRGTR
                     HNPGLNVGMGKDHTLFALIDGRVEFRKRRNDRSFVSVIPVEQMEKAETVSQ"
     gene            3289..4566
                     /gene="serS"
                     /locus_tag="PKM34_01790"
     CDS             3289..4566
                     /gene="serS"
                     /locus_tag="PKM34_01790"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ82342.1"
                     /translation="MLQIGYIRDNRDVVVSRLAIKNFKAEEIVGEIINLDEKRREIQK
                     KLDDTLSRSNQLAKEIGGLFQKGMAAEANAMKKQTAELKDLARELSRQLDETNARLND
                     QLVLLPNLPHNSVSPGKSAEDNEIVFEWGSLPDLGDKAKPHWELASGYDIIDFDLGSK
                     VTGAGFPFYKGYGAKLQRSLINFFLEEAIAAGYTELQPPLFVNEDSAYGTGQLPDKDG
                     QMYAIGLDKLYAIPTAEVPVTNVYRDVILKADDLPVKNVAYSSCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQISHPDLSYAQLEEMRLHVASLLQKLELPFRIVRLCGGDMSFTSA
                     LTYDFEVWSGAQKRWLEVSSVSNFENFQANRMKLRFKDEEGKTRLAHTLNGSALALPR
                     VVAALLENNQADDGIRIPAALQKFTGFEKIILK"
     gene            4544..6445
                     /locus_tag="PKM34_01795"
     CDS             4544..6445
                     /locus_tag="PKM34_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82343.1"
                     /translation="MKKSSLNKFYKTLFPLVVIAFITAMAPLNTFSQVFPEVSTPEAS
                     KKQQEQEKEKLAFQYYGNKEYEKALELFRDLYGIQPTYHRYTYYFYCMTYLNLFDEAE
                     KVVKGRMKAEPESYRYQVDLGYLYLLTDKPEKATKIFDEVLAGMKPTKAAVNELANAF
                     VMRQLYDYTIKVYLKGREILGYTEMFHQDLARIYEVSGNYSGMIEEYLDLLQADPMQL
                     ETVQNRLQNALNKDIDDKISNLLRESLLRRNQRNPDNRNYGEMLLWLSVQQKDFEFAL
                     IQARSLDTRFNEGGFLVLEVGDLALSNKEYDVAIDAFSYITRKGQMHPLYAECLTGSL
                     QARFLRLTEGYGSGEDNLEDLEKEYLTAIELLGKNARTLILMRDLSHLQAFYLNKLDD
                     AVALLEEILSIPNAPDLVKADIKLELGDIYLFVGEVWEASLLYSQVEKMFKNDPKGHE
                     AKFRNARLSYYIGEFDWAKAQLDVLKAATSKLIANDAMDLSLLIGDNMDADSTYTTLS
                     CFARAELLKYRNQDSLAVITLDSIAMMGLYNPLDDEVLFSKAEIFIRQKKFHLADSLL
                     ATLVKVYSRVILADNALFKRAEMQAEVFGNDPLALELYQQLMLDYPGSLFATEARKRF
                     RQLRGDFNN"
     gene            6457..7227
                     /gene="rsmA"
                     /locus_tag="PKM34_01800"
     CDS             6457..7227
                     /gene="rsmA"
                     /locus_tag="PKM34_01800"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNQ82344.1"
                     /translation="MNNFVRPKKHLGQHFLKDENIARKIVGSRQSAVGSLLEIGPGMG
                     VLTKYLLDLPGIECYFIDTDREAIDYLNNAFPGHEARFIHGDYLRMNTAKIFTGPYAV
                     IGNFPYNISSQIFFRILEERQNVTEVVGMVQKEVAERLAAGPGSKTYGILSVLLQAYY
                     RIEYLFTVNETVFVPPPRVKSAVIRLTRNETDKLDCDEELFFRVVKTAFNQRRKTLRN
                     SLKSFYIDYERGEILHLAGKRAEQLSVEDFVRVTRVAV"
     gene            complement(7269..>9733)
                     /locus_tag="PKM34_01805"
     CDS             complement(7269..>9733)
                     /locus_tag="PKM34_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ82345.1"
                     /translation="INNGRSFLNYRGEGWYDGWHANCYYFSVSDVAVLNNTNKTPFVT
                     SIGCGVAAFNASGGNCFGEEWMEMGSMSAPRGAAAFLGPTSNTHTAFNNEIDKGIYIG
                     MFDEGLETPGQALLRGKFNMYTVFGDVYWVEYHYKIYCCLGDPSIHIWKTIPQNVNVV
                     FPPTIPVGVSEAEIIVTTMFLAPVKNATVCVTGDEVFATGITDSNGVAVLTISSTEEE
                     LLTLTVRGGNVIPSQDTIETFQYDVFIEPAGDPVIDDLDGNNDGLVNPNEHCSVTYTL
                     SNWGVETAYDVQAVLTSEDTAFVEIITTDPVDFGNIYPSLSSSGSPFQIYIKPGCPVG
                     QNITLQLHITCDTSFWDYSHTENIKGCRIGIDQFIISDPCAAPDMNFRLDPGETDVVV
                     LSVKNTGEDLAPDLKGILRSDDPFITVTDSTGTFGTLNMGDLAMNMENVFMVSVSPSC
                     PADYMAGFTLKAFTQNGNYPYQTLIDFQIPVSKLIPSDYTGPDAYGYYAYSSDDAFYD
                     PRPEFNWIELEGGGTQIFLPQNSNYTTTLNIPFPFKYYGINYNQVRVSTDGWMAFGNG
                     TQVAPNNTELPMHDNVNNMVGVFWDDLYDDEFFMGNILYFNDVFGHRFIIEWDSISHN
                     GPTTSPVKEYFQAILLDPAYYPTTTGDGDIIVQYRIVSDNSSNTIGIENSTQDIGLQY
                     VFNTDYDPTASVLKDGLAIRFTTEPPLANILTGADELGREIANSGPEGTGKIRNHPNP
                     FNGSTTIEYTMPEQGRVVLAVYDIRGELVRDFYIGKQSPGVYTIEWNGLNNAGAPVSP
                     GIYFCTLQTDGYSETIKMFLLK"
BASE COUNT         2664 a         2279 c         2344 g         2446 t
ORIGIN      
        1 ccgttaaagt cgcgccggca atggccaggc cgtcataatt gaagacataa ccttcgactg
       61 ctccctgggc cggacagtta ccgtaaagtt gtccgggaag aatattcggc ggtggtacat
      121 atgaaccata gccatccgat acccaaacct gagcacccat gtagttaaat atataataat
      181 aacattcata tgcccatcca ccggcataaa agattgtagt tatcggtgct cccgagttta
      241 cagtaaaata gtaaattgca gggccggatc cactcgcaag ggtaatgtta tcaagtacaa
      301 ctgcaccatt aacaagtaca tccagatagc ctccattcca gccatctcca taatgatcgt
      361 aaagatcaat ccggtaatcg caggtgggca caggcccgga agtaccttcg aggcggaaca
      421 taaagtcgtt attggggtaa ccaatatttg ccatatacgt ccaggtcgtg gctccaagcc
      481 cgaacccgtt accgggatta cgccagcatg ctccgctgtt gtaaatggag gtagtcctgg
      541 tccagtacca ttgtccataa gttgcataag gcgatgcatc ctgcacggat acccagtaat
      601 gacccggacc caagacgata ttgcccggaa tattaatggt cagcacagca tttgcattgg
      661 tacaggtgag gttcatcagt gtagttgagg ctgaagcagc cggcatacct gcattgtctg
      721 cataaatgta gacatgtaca agtgtggccg tggtgcttcc gctacctgtt gccgtaatgg
      781 tctggatggt ccattgcatg ccggcaggca cgtaaaagtc gtctgccccc tggcaatcat
      841 aggcatcgta cacagtttcg aaatcctgcg aggccatccc ggctccgctg gagttcgtct
      901 gctgatagag aactgccctg gattcaggga gtaccggaga cttgcctccg ctgtttgtca
      961 cgcgcagctg ggcaagcacg acagacgatg atatcaacaa tatcatcatc gtcagtagat
     1021 acttggaaaa gtttctcatg gtaattgaat taagttaatt aatgtgtttg tttttggttg
     1081 ttctgtcaat gggtaagtaa actgtttggt gattacatat atatatttag atgaaataac
     1141 cctaaggagg ttccggattt tgtaataata tatcaataaa aacagacatt ggcgttatct
     1201 attttttttt gaaccgttta taaacctgga cccttcaaat ataaacagaa aaatctattc
     1261 aataatacat tactatgact tttttcaacc ggcatggcta atttatattc attttacaaa
     1321 agctctatta atgagggatt tatcaagtct ttgtttcaaa acggcaaagg attgttaata
     1381 tcttggcctt ccgaaaaaaa acgggatgat ctttttctat ttttgttgat aaatgagagc
     1441 ggaaaaatct ttgacaaaca gcagtttatt tccctggatc atcctggtga tactggctgt
     1501 tatctgggga agttcattta ttttgattaa aaaagggctg aattatttta caagcgttga
     1561 agtgggtgca ctcaggatat ctatcgcctt cttgttttta ttgccttttg tttttaaaag
     1621 aataagaaga ttgagccgaa gggagtggaa atttctgatc ctgaatggca tgatcggaag
     1681 cggtttccct gcatttttat ttgccaaagc ccaaacaggc atcgacagtt cgctggccgg
     1741 catcctgaac tcccttaccc cccttttcac gcttattgtc agcctcagtt ttttctcact
     1801 aaaaatcaga tggtttaaca tcctgggcgt tattatcggg ttgatcggcg cgatcgggct
     1861 gatcagcatc agtggcggaa aaagttttga tttcaatttc ggttacgcgg tctatataat
     1921 ttttgccacg atttgttatg caaccaatgt caatatcgtc aaatataaat taaaagacac
     1981 tgacgcgctt accatcactg tattctcgtt ttttaccatc ggtattccaa tcctgctgta
     2041 ccttctgctg ttcaccggtt ttataaataa aatgacgaca atcccaacct cctgggaagg
     2101 cctgggctac attggcatcc tggctgtggt cgggaccggt atggccctga tagccttcaa
     2161 taagctcgtc aagattgcaa gccccgtttt tgctgcatcc gtcacctacc tgataccggt
     2221 cgttgccgtg tcatggggaa tgattgacgg ggagaaaatt actattctcg cctttttttg
     2281 gatgatggtc atcctgttag gggttttcct ggtcaataag aaaaaagcgg ttaagttgta
     2341 aatttttaaa gatttacttt gtcggattaa aaaatgttgt acttttgcgc ttccaaattt
     2401 tgaccgacag aaaaatataa atgtaatgta cgcaatagtt gatatagccg gacagcaatt
     2461 caaggtggag aaaggacagg agatctttgt gcatcgtctt gaaggggaaa aaggggcaaa
     2521 gttagacttt gaaaaagtga tgctgatcga ccacgacggg aagactgttg tcggtacacc
     2581 ggtcatcgac ggagcgatgg tccacggcac gatcatggat catgtccgcg gcgataaagt
     2641 gtttgttttc aagaagaaac gtcgtaaagg cttcaaaaaa ttcaacggtc accgccagga
     2701 tttaagcctc atcaaaattg aagacattgt agaaaaagtg acccggtcgc gttcgaagaa
     2761 agcagcggca gcccctgcag aagaagcagt tcaggcagaa ccggcacagg aaaaccagta
     2821 attgattcag taacacctta aaactcagaa tatggctcac aaaaaaggtg caggaagttc
     2881 atccaacggc cgcgaatcgc acagcaaacg gcttggagtt aagatatacg gcggacagtt
     2941 tgccaaagca ggcaatatta tcgttcgtca gaggggcact cgccacaatc ccggactgaa
     3001 tgtcggcatg ggaaaggatc ataccctctt tgcgctgatc gacggcaggg tggaattccg
     3061 taaacgcagg aacgaccgct catttgtctc cgttatccct gtcgaacaaa tggaaaaagc
     3121 tgaaacggtc agccagtaaa tcgacccaat aatcatccct tataaacgaa ctcaccccgc
     3181 agagatgcgg ggtttttttg tgccactgcc actgcccctc ccccaaatcg gtggagtgat
     3241 cggtttattt tactaataat cgtacctttg cacctcaatt ttcagaatat gcttcaaata
     3301 ggatacatca gggataaccg ggatgtcgtg gtcagtcgcc tggccataaa aaatttcaaa
     3361 gcggaagaaa ttgtcggtga aattattaac ctggatgaga aaagaaggga gattcagaag
     3421 aaacttgatg ataccctttc caggtcaaac cagctggcaa aagagatcgg tggtctgttc
     3481 cagaaaggaa tggcagccga agccaatgcc atgaaaaaac aaactgccga actcaaagat
     3541 ctggcccgcg agctctcccg gcaactcgac gagaccaatg cccggctgaa cgaccagttg
     3601 gttctactcc ctaatctgcc gcataattcc gtatcgcccg gaaagagcgc cgaagacaat
     3661 gaaatcgttt tcgaatgggg ctccctgcct gatcttggcg ataaagcaaa acctcattgg
     3721 gaactggcat ccgggtacga cattatcgat tttgaccttg gaagcaaggt gaccggagca
     3781 ggatttccct tttacaaagg ttacggcgcc aaactgcaac ggtcgctcat caatttcttc
     3841 ctggaagaag cgattgccgc aggctacacc gaacttcagc ctcccctctt cgtcaatgag
     3901 gattcggcct acggcaccgg ccagttgccc gacaaagacg ggcagatgta cgccatcggg
     3961 ctggacaagc tttacgcgat cccgacagcc gaagtacctg ttacgaatgt gtaccgcgat
     4021 gtcatcctga aagccgacga cctgccggtg aagaatgtcg cctattcgag ctgtttccgt
     4081 cgggaagccg gatcctacgg caaagatgtc aggggattga accggctgca ccagttcgat
     4141 aaagtcgaaa ttgtacagat atcgcatccc gatctctcct acgcccaact cgaggagatg
     4201 cggcttcatg tcgcatcgct tctgcaaaag ctcgagctgc cgttccggat tgtcaggctt
     4261 tgcgggggcg atatgagctt tacctctgca ctgacctatg atttcgaagt atggtcgggc
     4321 gcgcaaaagc gatggctcga agtgagctcg gtgtcaaact tcgagaattt ccaggccaac
     4381 cggatgaagc tcaggtttaa agatgaagaa gggaaaacac ggctggcaca cacactaaac
     4441 ggcagcgccc tggcgttacc cagagtggtc gcagcattgc tggaaaacaa ccaggcggat
     4501 gacgggatca ggatcccggc ggccctgcaa aaattcaccg gttttgaaaa aatcatcctt
     4561 aaataaattc tataaaacct tattcccttt agtggtcatc gcattcataa cggcaatggc
     4621 cccactgaat acgttttccc aggtttttcc ggaggtcagt acccccgagg cttctaaaaa
     4681 acagcaggaa caggaaaaag agaagctcgc tttccagtac tacggaaaca aggaatatga
     4741 aaaagcgctc gaattattcc gagatctcta cggtattcag ccaacctatc accggtatac
     4801 ctattatttc tattgcatga catacctcaa cctgtttgat gaagcggaaa aggtggtcaa
     4861 aggaaggatg aaagccgaac ccgaatcgta ccggtaccag gttgatctcg gttatctata
     4921 tctcctgacg gataaaccgg agaaagcaac taaaatattt gacgaggtac tggccggcat
     4981 gaaaccgacc aaagcagcgg ttaacgaact ggccaatgct tttgtgatga ggcagttata
     5041 tgattatacg ataaaggttt atctcaaagg gcgggaaata ctgggctaca ccgaaatgtt
     5101 ccaccaggac ctggccagga tctatgaagt cagcggcaat tacagcggga tgattgaaga
     5161 atacctggat ctcctgcaag ccgatcccat gcaactggaa acggttcaaa acaggctgca
     5221 gaatgcactg aacaaagaca tcgacgacaa gatcagcaac ctgcttcggg aatcgctgct
     5281 gagaagaaac cagcgtaatc ccgacaaccg caattacggc gaaatgctgc tctggctttc
     5341 cgttcagcag aaagacttcg aatttgcgct gatccaggcc cggtcgctcg atacgcgctt
     5401 caatgaaggc ggtttcctgg tgctggaggt aggcgacctg gcgctgtcga acaaagaata
     5461 tgacgtggcg attgacgcat tctcctacat tacccgcaaa ggtcagatgc atccgctgta
     5521 tgccgaatgc ctgaccggat cgctccaggc caggttcctg agactgaccg aaggttacgg
     5581 aagcggggaa gataacctgg aagaccttga aaaagaatat ctgacagcca tcgaacttct
     5641 cggcaagaat gcccgtacct tgatcctgat gcgtgacctg tcgcatctgc aggccttcta
     5701 cctcaacaag ctcgatgatg cggtagcact gctcgaagag attctcagca tcccgaatgc
     5761 ccccgacctg gtaaaggccg acatcaagct ggaactcggc gatatctacc tgttcgtcgg
     5821 ggaggtgtgg gaagcatcgt tgctttattc acaggtagaa aaaatgttca agaacgatcc
     5881 gaaagggcat gaagccaagt tcaggaatgc ccgcctgtcg tactatatcg gcgaattcga
     5941 ctgggctaaa gcgcagctcg atgtgctgaa agccgccaca tccaagctga tcgccaacga
     6001 tgcaatggat ttgtcgttgc tgatcggcga taatatggat gccgacagca cttacaccac
     6061 cctttcctgc tttgcgcggg ccgaactgct caaataccgc aaccaggaca gcctggcggt
     6121 aatcaccctc gattccattg ccatgatggg gctttacaat cccctggatg atgaggtttt
     6181 attcagcaag gcagaaatct ttatccggca gaagaaattt catctggccg actcactgct
     6241 ggctaccctc gtcaaagtat attcgagggt tatcctggcc gacaatgcgc tgtttaaaag
     6301 agcggagatg caagcggaag tattcgggaa tgatccgctt gccctggagc tttaccagca
     6361 gcttatgctc gattatcccg gcagtctttt cgccaccgaa gccagaaaac gtttccggca
     6421 gctgcgcggc gattttaata attaagaact ttttgtgtga ataattttgt caggccgaaa
     6481 aagcacctgg ggcagcattt tctgaaagat gagaatattg ccaggaaaat agtcggcagt
     6541 cggcagtcgg cagtcggcag tttgcttgag atcgggccgg gaatgggggt gttgacgaag
     6601 tacctgctgg atttgccggg aattgaatgc tatttcatcg atacggacag ggaggcgatc
     6661 gattatttga ataacgcgtt tcccggtcat gaggcgcgat tcattcacgg tgattatctc
     6721 aggatgaaca cagcgaagat ttttacaggg ccttatgctg ttatcgggaa ttttccatac
     6781 aatatttcga gccagatatt tttccggatc ctggaagaga ggcagaacgt aacagaggtc
     6841 gttggaatgg ttcagaaaga ggttgcggag aggcttgccg cgggaccggg atcgaaaacc
     6901 tacggcatcc tgagcgtatt gctccaggcc tattaccgga tcgaatacct gtttacggtg
     6961 aacgaaaccg tcttcgtccc gccacccagg gtcaaatcgg ctgtgatacg gctgacgaga
     7021 aacgaaacag ataagcttga ctgcgatgaa gagttgttct tccgggtggt caagaccgct
     7081 ttcaaccagc gccggaaaac gctccggaat tctttaaagt cgttttatat cgattacgaa
     7141 cgcggtgaaa tccttcacct ggccggcaaa agggcagaac agctcagtgt ggaggatttt
     7201 gtgagggtga cgagggtagc ggtgtgattc atgttccgtt ttgcatttta cgttttacat
     7261 ttttcatttt atttcaaaag gaacatttta atcgtttccg aatacccatc cgtctgcagc
     7321 gtgcagaaat aaatccccgg gctcaccggc gctccggcat tgttcaagcc gttccattca
     7381 atggtataaa cgcctggaga ttgtttcccg atgtagaaat ccctgaccag ttctcccctg
     7441 atatcataga ctgccaggac caccctgcct tgttccggca tggtatattc gatcgtggtg
     7501 gacccgttaa aaggattcgg atgattccgg atcttaccgg ttccttccgg ccctgaattg
     7561 gcaatttctc ttcccaattc atcagctccg gtaagaatat tggctaatgg cggttcggtc
     7621 gtaaaccgga tagccagccc atcctttaaa acagaagctg tgggatcgta atcggtattg
     7681 aagacatact gcaggccgat atcctgggta ctattctcga tcccgatcgt attgcttgaa
     7741 ttatccgaca cgatcctgta ttgcacgata atatcgccat caccggtagt cgtcggataa
     7801 taggccggat caagcaatat cgcctggaag tattctttaa cgggtgaggt ggtcggtccg
     7861 ttatgggaaa tgctatccca ctcaataata aaacgatgac caaacacatc attaaaatac
     7921 aaaatattgc ccataaagaa ctcatcatca tataaatcat cccaaaaaac gcctaccata
     7981 ttatttacat tatcatgcat cggcagttcc gtattattgg gtgccacctg ggtaccgttt
     8041 ccaaaagcca tccatccatc ggtactgacc ctgacctggt tataatttat tccataatat
     8101 ttaaagggga aggggatatt tagcgtcgta gtataattgc tgttctgggg cagaaaaatc
     8161 tgggtaccgc ctccctccag ctcgatccag ttaaactccg gccgcgggtc gtagaaggca
     8221 tcatcgctgg aataggcata atatccgtat gcatcggggc cggtataatc agacggaata
     8281 agcttggaaa ccggaatttg gaaatcaatc agagtctgat aaggataatt tccattctga
     8341 gtaaaagcct ttagggtgaa accggccatg taatccgccg ggcaggaagg gcttacgctg
     8401 accatgaaaa cattttccat attcatggca agatcgccca tgttcagtgt accgaatgtt
     8461 ccggtagaat cggtcacggt aatgaaggga tcatcggatc ttaaaattcc tttcaggtca
     8521 ggcgccaggt cttcaccggt atttttaaca gaaaggacga ccacgtccgt ttcgccggga
     8581 tcgaggcgga aattcatgtc gggggctgca cacggatcag aaataataaa ttgatcgatc
     8641 ccgatcctgc atccttttat gttttccgta tgggaatagt cccagaagct ggtatcacag
     8701 gtaatatgca gttgtaaagt aatattctgt ccgacagggc agccgggttt gatataaatc
     8761 tggaacgggc ttccggaaga agataatgaa ggatagatat tcccgaaatc caccggatcg
     8821 gtggtgatga tctccacaaa tgcagtgtct tcggatgtta atactgcctg cacatcgtag
     8881 gccgtttcaa ctccccaatt tgaaagggta tatgtaacac tgcaatgttc attcggattc
     8941 accaggccat cgttatttcc gtcaaggtca tcaatgacag gatcgcctgc cggttcgatg
     9001 aaaacatcat actggaaggt ttcgatcgta tcctgggaag ggatgacatt tccaccacgc
     9061 accgtcaaag taagcagttc ttcctccgta gaggagatcg taaggacagc cacgccattt
     9121 gaatcggtga tgccggttgc aaacacttca tccccggtca cacaaacagt ggcattttta
     9181 accggcgcca ggaacattgt tgtcactatg atctcagcct cactgacccc aacagggatg
     9241 gttggcggaa aaacgacatt aacattctgg ggaatggttt tccagatatg aatactcgga
     9301 tcgcccaggc agcaatagat cttataatgg tattccaccc agtaaacatc tccaaaaacg
     9361 gtatacatat tgaatttacc tctcaggagg gcctgacccg gtgtttcaag gccttcgtca
     9421 aacatcccga tataaattcc cttatcaatt tcattattaa atgcggtatg tgtattcgac
     9481 gtaggaccga gaaatgcggc agcgcccctg ggagcgctca tggaacccat ttccatccat
     9541 tcttcgccaa aacaattccc gccggaggcg ttaaatgctg ccaccccgca acctatactc
     9601 gtgacaaaag gggttttatt cgtattgtta agtactgcga catcggaaac ggaaaagtag
     9661 tagcagttgg cgtgccatcc atcgtaccat ccttcgccgc ggtaattgag gaaactgcga
     9721 ccgttgttaa tcg
//
LOCUS       DAOQXS010000032         9701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7504_length_9701_cov_4.269749, whole genome shotgun sequence.
ACCESSION   DAOQXS010000032 DAOQXS010000000
VERSION     DAOQXS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7504_length_9701_cov_4.269749"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKM34_01810"
     CDS             <1..637
                     /locus_tag="PKM34_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNQ82346.1"
                     /translation="QSFLKLQSEGYSYSINHDEGINIEKLDKGCLILNTQNDKGYKIC
                     LVDKSNKSAETQYWKDDFLKVKPIANEFFQTNQFLGVAKNFVTKQFSKEFEVSRAEQI
                     DLLNKSVEYFKLHETFDKNEFETDVFKNQEVIDSFRNFDSQFRQDNDIELSDQFDISQ
                     KAVKKQARIFKSVLKLDKNFHIYIHGDRELIQQGVDPDGRKYYKIYFKEES"
     gene            complement(641..1564)
                     /locus_tag="PKM34_01815"
     CDS             complement(641..1564)
                     /locus_tag="PKM34_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ82347.1"
                     /translation="MPIPTSRTYESGHVVIDQEKCTGCGLCAEVCSDAVIEIIDGKAV
                     ATQKNSVFGCIGCGQCMAVCPEGAVSVTGRWISPDDVFPLADRSESASYDSLLKLYQR
                     RRSIRDFKDKPVEPELVEKILQAAVTAPMGIPPSDVNVLVLNSKEKVFQFSKDYCDYL
                     ENLKWMVSPLGLLFMRLFYGKENGDMFRDFIKPVINAYTGNMKEGKNIVMYDAPLAFY
                     FYGSPYTDPADPIIACTYAMHAAESLGLGTCMLGAIHPFIQSGGAAKKFREKHGIRFK
                     SKEGLFLIVGYPKVKFRNGIKRTFAGVEGLN"
     gene            complement(1691..2614)
                     /locus_tag="PKM34_01820"
     CDS             complement(1691..2614)
                     /locus_tag="PKM34_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ82348.1"
                     /translation="MQTILGSTGIIGKEVARELYRSYTKDIRLVSRNPKPVNPTDQLF
                     KADLLDRDQVRRAVEGSEIVYLTVGIQYSAKLWAEQWPVIMRNVIEACKAEKAKLVFF
                     DNVYAYGKVDGVMTEETPYNPCSKKGTVRLKVINMMMDEVRKGNLEGLIARAPDFYGP
                     DTPLSVVSVTVFGNLAKGKEPQWMVDDRKKHSFIFTPDAGKATALLGNTPSAFNQVWH
                     LPTDSNTLTGKEFVVLAARAFGMKPSYMVLPKWLLRTVGLFNPIIRESLEMLYQNEVD
                     YIFDSSKFEKAFGFAPTPYPTGIEETARSYK"
     gene            complement(2669..4747)
                     /gene="fusA"
                     /locus_tag="PKM34_01825"
     CDS             complement(2669..4747)
                     /gene="fusA"
                     /locus_tag="PKM34_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ82349.1"
                     /translation="MPLNRLRNIGIAAHIDAGKTTVTERILFFTGVTRKIGEVHDGQA
                     TTDFMKQEQERGITIASAAISCAWKDSQINLIDTPGHVDFTVEVERSLRVIDGMIALF
                     CAVGGVEPQSETVWNQADRYKVPRIAFINKMDRTGADFYDVVGQMNNVLDANAIAFQI
                     PIGVEDDFTGIIDIISGKAYKFTDKGREEISIPGEYTEKVNEARHQLIEKLADFNDEI
                     AELFLDDGEISNELLKKAAREATLRLNITPVFCGAAYKNKGVQLLLDAVIDYLPSPLD
                     IGAIVGTDVDNPEKSHARHPSANDPFSALAFKLINDPYVGQQTFVRVYSGQLKSGMQV
                     LNSTKDKTERIGRIMKIHAKDREEISDAGPGDIVALVGLKLTKTGDTLCDPAHPLHLE
                     SITVPATVIEMKILPTSRKDQSKLGEALHKLSNEDPSFNVRYDEETEETIIAGMGELH
                     LEIIIDRLKHEFGVEVDVSEPSVAYRETITTEVEASHRYSKQTGGKGQFAETHFRIEP
                     NEGNGYEFIDRIKGGAIPMEFVPSVNKGITKTLEKGVLAGFPVVDVRIVLHDGKFHPV
                     DSSDMAFQTCASICFKEGFMKAHPILLEPIMKVEINTPDDYIGDIVGNLNKRRGKVES
                     MRRHRKGSQKVVAFVPLVEMFGYATQLRNISSGRASFSMDFFRYMPLSKTVQEEVLKK
                     LAEKKAAMKK"
     gene            complement(4912..5517)
                     /locus_tag="PKM34_01830"
     CDS             complement(4912..5517)
                     /locus_tag="PKM34_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNQ82350.1"
                     /translation="MKTLLLISIFIFLAYTGKTQTVTDTDGNTYPTVKIGEQTWMAKN
                     LAVTRAPDGTVLESYPCNGIEDSVKTFGRMYDWANAKKACPAGWHLPTDEEWTQLVDF
                     LGGPMIAGGKIKETGYAHWKAPNAGADNKSGFTALPGGYRNPKGKYFNFKNNLAYFWT
                     ATEYDEANAWGYYITYGEPIIYRYGMSFGKKMAFAVRCIKD"
     gene            5640..9047
                     /locus_tag="PKM34_01835"
     CDS             5640..9047
                     /locus_tag="PKM34_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82351.1"
                     /translation="MKAVFSLVCLTMFYSSSVVAVTRYWINGGSGNFNDGTHWSLSSG
                     GNPINGTISWAITDVAVFDYNSGSPTVIFTASEQIGKFRFYGSDTVTLKANSTYSRTL
                     GINAVDTASLLVSDVSTLIISGTASTTDRNMTVELDNATGVTAKIYGVVEVKEENSGY
                     GEFRKKGSYSTIFFRYGSTYIHNTKETGSDIPIATWHAASTCKITGITGTVPGNLDQP
                     FGNLIWNCPGQTDVFNWPTMPALLGNFTIASTGSGGIKLSDAIDITWNISGSYSQTGG
                     NFYPAGTSANNIINLGGGFSMTGGILSCPGTGSCLFRFNTSGVHNYSKTGGTYYQKIH
                     FEVVSGATLDMGGSIIDNGSSGNFTLNSGGCLKTAHVQGISSTGASGCIQLTGSRTFN
                     AGASYIFYKSGSQATGTGLPATLSSVLTIGTIHNATSLTLTNSPVTINGTLVLVSDSA
                     INSAIASGTISYGSNGTLEYQGDTAQVTTDIEFPSTSGPYNLKINNAYGVSLHEDRTI
                     NGTIYLSIGQFLIGAHTLTINGTLSQTMGVLKGGLSSNIIVGGTGNLLTLPPVMLNDL
                     TLNRPAGMTGTGLDTVYGRFTLTQGTLWPAGVLVYGPEATLKYNGSSAQTTTSAEFPE
                     NYGPCNLENSNPIRLDLHSSRIIQGNLQLNSGIFSIGSNTLTINGLITGPGTLLGGSS
                     SDIVFGGSGPATTLPGITLSDLTIDRASGINMGGTVTITNSLNLLEGDFSIGFNTLIL
                     NDELNYDGGNLTGSLSSRLIIAGQGDPVSLDAITLNELNLSRPSILYLEDNILVEGIL
                     ILSAGNIYRQGHTLYGSDATLIYYGATAQATSGEEWPWNDGPHHIIIQNSQGVYLDSS
                     RKVTGDLTLAKGDLHIGGNTLILEGIINPVMGNLSGGTESALEIEGNGDGTQIPSVDL
                     RKLKINRQTGAWLAGVCTIYDSLHLVAGNLMVNNQTLILQGGPVTGDPNNLITEPVSN
                     LVFAGDHTGVSIPESITDLNYLAVSNPNGVVLQSDILLNSSCDVTGYMICGEHSLSGN
                     AGVNILPGARFATAHPAGISGTIGVALPSFLSSGADYEFNGNYPQETNFLETSTPGLI
                     RNLIVNTAEGIPLVLTGNITTSEGVHVSSGSILTIPDEITLTAGNPE"
     gene            9288..>9701
                     /locus_tag="PKM34_01840"
     CDS             9288..>9701
                     /locus_tag="PKM34_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNQ82352.1"
                     /translation="MLNKIISFLLLIFMVPLISLAEWIPLNSSNSADPATPKLTLLHQ
                     NARETIIKIELSGFEMNDFMVGRTTYQAVNILSEALTHTPGQPELPYVATILAIPDQA
                     SVRVEVVETGQESTFRNVYLPPARKSWYEGDTEPSY"
BASE COUNT         2422 a         2254 c         2429 g         2596 t
ORIGIN      
        1 gcaatctttt ttaaaacttc aaagtgaagg atatagctat tctataaatc atgatgaagg
       61 gattaacata gaaaagttag ataaagggtg tttaatttta aatactcaga acgataaagg
      121 atacaaaatt tgtttggtag ataaatcaaa taaatcagcc gaaacccaat attggaagga
      181 tgacttttta aaggtcaaac cgattgcaaa tgaatttttt caaacaaatc aattcttagg
      241 tgtagctaag aactttgtaa ctaaacaatt ttcaaaggaa tttgaagtta gcagagctga
      301 gcagatagac ttactgaata aatctgtgga gtatttcaaa ttacatgaga cttttgataa
      361 aaatgaattt gaaacggatg tttttaaaaa tcaggaagta attgattcgt ttagaaattt
      421 tgattctcag tttagacaag ataatgacat tgagctttct gatcagtttg atatatcgca
      481 aaaggcggtg aaaaagcaag caaggatttt taaaagtgtc cttaagctgg ataagaattt
      541 tcacatatat atccacggag atagagagtt aatccagcaa ggtgttgatc ctgatggccg
      601 gaagtattat aaaatctact ttaaagaaga aagctgattt tcagtttaag ccttccaccc
      661 ccgcaaacgt ccttttaatc ccgttccgga atttaacttt tggataacca acgatcagaa
      721 acaggccctc cttgctttta aaccggatgc cgtgcttttc gcggaacttc tttgcggcgc
      781 cgcccgactg gatgaacggg tggatggcgc cgagcatgca ggtgcccagc ccgagcgatt
      841 cggcggcatg catggcgtaa gtgcaggcaa tgatcggatc ggcgggatcg gtgtagggcg
      901 aaccgtaaaa atagaaagcc agcggggcat cgtacatcac aatgttcttc ccttctttca
      961 tgtttccggt ataggcgttg atgaccggtt tgatgaagtc gcggaacata tcgccgttct
     1021 cttttccgta gaacagccgc ataaaaagca ggcctagggg cgacaccatc catttcaggt
     1081 tttcaaggta atcacaataa tcttttgaga actgaaaaac cttctcttta ctattcaata
     1141 ccagcacgtt aacatccgac ggcggaatgc ccatcggagc ggtaaccgcg gcctgaagga
     1201 ttttttcgac cagctccggc tccacgggtt tgtctttgaa atcgcggata ctgcggcggc
     1261 gctggtatag ctttagcaac gaatcatacg aagccgattc cgatcggtcg gccagcggga
     1321 atacatcgtc aggggaaatc catctcccgg tgacgctgac tgctccttcg gggcagacag
     1381 ccatgcactg gccgcatccg atacagccga agacggaatt tttctgggtt gcgacggctt
     1441 tgccgtcaat tatctctata accgcatcgg agcaaacttc ggcacataat ccgcagccgg
     1501 tgcacttttc ctggtcgata acaacatgac ccgactcata ggttctggag gttggtatgg
     1561 gcataatcgg tttgatttat gccttaaaat taataataat ccgcgttgat ccgccgcatc
     1621 cgtgtttatc cgcgtgctat cagcaataat agcactcgga tgatgcggat tatgcggatt
     1681 aacacggatc ttatttgtac gatctggcag tctcctcaat tccagtcgga tatggagtcg
     1741 gcgcaaaccc aaacgctttt tcgaatttgc tgctgtcgaa aatgtaatcc acttcgttct
     1801 ggtagagcat ttcgaggctt tcccggatga tggggttgaa taatcctacg gttctgagca
     1861 accattttgg gaggaccatg tacgatggct tcatgccgaa ggccctggct gccagtacga
     1921 cgaattcttt cccggtgagg gtgttggaat cggtcggaag atgccagacc tggttgaaag
     1981 cggaaggggt gttgcccagc agggcagtgg ctttacccgc atcgggcgtg aagatgaacg
     2041 aatgcttttt acggtcatca accatccact ggggttcctt gcctttggcc aggttgccga
     2101 aaaccgttac gctcacaacg ctcaacggcg tgtcggggcc atagaagtcc ggggcgcggg
     2161 cgatgagccc ctccaggttg cctttcctca cctcatccat catcatattg atcaccttca
     2221 gcctcactgt tccttttttg ctgcagggat tgtacggcgt ttcctcggtc atcacgccat
     2281 ctactttgcc ataggcataa acattgtcga agaaaacgag ttttgctttc tcagccttgc
     2341 aggcttcgat gacgttccgc atgatcacgg gccattgctc ggcccataat tttgcgctgt
     2401 actggatacc gaccgtcagg taaacgattt cggaaccttc aacagcccgc cgtacctggt
     2461 ctctgtcgag cagatccgct ttgaaaagct ggtcggtcgg gttgacgggt ttcgggttac
     2521 gggataccag acgaatgtct ttggtgtagg aacgataaag ttcgcgggca acttctttgc
     2581 cgatgattcc ggttgagcct aggattgttt gcatatttct ttttgttttg gcattgtcag
     2641 gtcctgcgga cgctgacaag tcgaaaaact atttcttcat cgctgctttt ttctcggcca
     2701 gtttcttcag cacctcttcc tgcaccgttt tgctgagtgg catgtagcgg aagaaatcca
     2761 tggagaagct ggcgcggccg ctggaaatat tacgcaattg tgtagcatag ccgaacattt
     2821 ctaccagcgg gacgaaggcg acgaccttct gcgagccttt gcggtgacgg cgcatcgatt
     2881 cgactttgcc gcggcgcttg ttgaggttgc cgacaatatc gccgatgtaa tcatccggtg
     2941 tattgatctc gaccttcatg atcggttcga ggaggatcgg gtgtgctttc ataaatcctt
     3001 ccttgaagca gatcgaggca caggtctgga atgccatatc gcttgaatcg accgggtgga
     3061 atttcccgtc gtggagcacg atccggacgt cgaccaccgg gaatccggcc aggacccctt
     3121 tttcgagagt cttagtaatt cctttgttaa cggatgggac aaattccata gggatagctc
     3181 ctcccttgat cctgtcgata aactcgtacc cattgccttc gttaggctcg atgcggaagt
     3241 gggtctcggc gaactggcct ttgcccccgg tctgtttgct gtagcggtgc gaagcctcga
     3301 cttctgtggt aatagtttcg cgataagcga cagagggttc actgacatcg acctccacgc
     3361 cgaattcgtg tttgaggcgg tcgatgatga tctccaggtg aagttcaccc atgccggcaa
     3421 tgatcgtctc ttccgtttcc tcgtcgtacc ttacgttgaa cgacggatcc tcattggaaa
     3481 gcttgtggag cgcttcgccc agtttgctct ggtctttccg tgaggttgga aggatcttca
     3541 tttcgatgac cgttgccggg actgtgatcg attccaggtg gagcggatgg gccggatcgc
     3601 agagcgtatc gccggttttg gtcagcttca gtcccaccag ggctacgatg tctccggggc
     3661 cggcatcgct gatctcttcg cggtctttgg cgtggatctt catgatgcgg ccgatccgtt
     3721 ctgttttgtc tttggtggaa ttcagcacct gcatgccgct tttcagttga ccggaataga
     3781 cacggacgaa cgtttgctgt ccgacatagg ggtcgttgat cagtttaaat gccagggcgg
     3841 agaaaggatc gttggctgaa gggtgccggg cgtgcgattt ttcagggttg tcgacgtcgg
     3901 ttcccacaat ggctccaata tcgagcgggg aggggaggta gtcgatcaca gcgtcgagca
     3961 ggagctggac gcccttgttt ttgtaggctg caccgcagaa caccggtgtg atgttcaggc
     4021 ggagggttgc ttcgcgggcg gcttttttaa gcagttcgtt gctgatttcg ccgtcatcca
     4081 ggaaaagttc ggcgatctca tcgttgaagt cggccagttt ctcgataagc tggtggcggg
     4141 cctcgttcac tttttcggtg tattcgccgg ggatggatat ttcttcgcgt cctttgtcgg
     4201 tgaatttata agctttaccg gatatgatgt cgatgatgcc ggtaaaatcg tcttcgaccc
     4261 cgatcgggat ctggaaagcg atggcgttag cgtcgaggac attattcatc tggccgacga
     4321 cgtcatagaa gtcggcaccg gtgcggtcca ttttattgat aaatgcgatc cggggaacct
     4381 tgtaccggtc ggcctggttc catactgttt cgctctgcgg ttccacgcct cccacggcgc
     4441 agaacagggc gatcatcccg tcgatcacgc ggagggagcg ttccacctcc acggtaaaat
     4501 cgacgtgtcc gggagtatcg atgaggttta tctgggaatc tttccaggca catgaaatgg
     4561 cggcagaggc aatggtaatg ccacgctcct gctcctgctt catgaagtcg gtcgtcgcct
     4621 gcccgtcgtg aacctcgcca attttgcgtg tgacacctgt aaagaataat attcgttcgg
     4681 taacggtagt tttaccggca tcgatatggg cggcaatgcc gatgttgcgt aatctgttta
     4741 aaggcacaat ggtaattttt aaatgttgaa ttttgaatgt gccccctccc cgggcccctc
     4801 ccccaattgg gggaggggat caaggggtgg gggccgaaaa agtttgcaaa ggtagtgata
     4861 atcaagttgc aaaacgcaaa cttcatatga aagataacaa atatttaatt tttaatcttt
     4921 tatgcacctg acggcgaagg ccatcttttt gccgaatgac atgccatagc ggtagatgat
     4981 cggctcaccg taggtgatgt aataacccca ggcattggct tcatcgtatt ctgtcgcggt
     5041 ccagaaatag gccaggttat tcttgaaatt gaagtatttt ccttttgggt tcctgtaacc
     5101 accgggcagg gcggtgaatc ccgatttgtt gtcggcgccg gcattggggg ctttccagtg
     5161 agcatatccg gtctctttga tcttgcctcc ggcgatcatg ggcccgccca ggaaatctac
     5221 caattgggtc cattcttcat cggtgggcag gtgccatcct gccgggcagg cttttttggc
     5281 attggcccag tcgtacatcc ggccgaaagt ttttacgctg tcttcaattc cattgcaggg
     5341 atacgattcg aggaccgtgc cgtcgggcgc tctggtgacc gccagattct ttgccatcca
     5401 ggtctgttct ccaattttga cagtggggta agtgttaccg tcggtatcgg tgacggtctg
     5461 ggtttttcct gtatatgcca gaaaaataaa aatggaaatc agcaggaggg ttttcatcac
     5521 accgtatttt taaacgaagt tataaaaaca ctttaaaccg aaattaggtt ttcttaattt
     5581 aagttttcaa atttcataac tttaatgttt ttaaaggatt taattaaaaa ggaggaatta
     5641 tgaaagcagt tttctccttg gtttgtctta ctatgtttta ttcttcctct gttgttgcag
     5701 ttaccaggta ctggatcaac ggcggttccg gcaatttcaa cgacgggaca cactggtcgc
     5761 tttcttcagg aggaaatcca ataaacggta cgataagctg ggccataaca gatgttgcag
     5821 tatttgatta taattcgggt tctccaacag ttatcttcac tgccagcgaa cagatcggga
     5881 aatttcgatt ttacgggtcc gatacggtca ccctgaaagc caacagtaca tacagccgta
     5941 cgttaggcat caacgctgtc gacaccgctt cattacttgt cagcgatgta tcgacactga
     6001 tcatttccgg cacggcttcc actactgatc gaaatatgac cgtggagtta gacaatgcga
     6061 cgggggttac agcgaagatt tacggcgtcg tcgaagttaa ggaggagaat tcgggttatg
     6121 gagagttccg taagaaagga tcatactcca cgatcttttt cagatacggg agtacctaca
     6181 tacataatac aaaagagacg ggcagcgata tccccattgc aacctggcat gccgcctcaa
     6241 cctgcaaaat aacggggatt acgggcaccg tgcccggaaa cctggaccaa ccctttggaa
     6301 acctgatttg gaactgcccc gggcagacag atgtttttaa ctggccgacc atgcctgcat
     6361 tgctgggtaa ttttaccatt gcgagcacgg gaagtggagg gataaaatta tcggacgcta
     6421 tagatattac ctggaatata agcggaagtt attcacagac agggggtaat ttttatccag
     6481 ccggaaccag tgcgaacaac atcataaacc tggggggagg atttagcatg acaggtggga
     6541 tcctatcctg cccgggaaca ggcagttgcc tcttccggtt taatacttcc ggagtacata
     6601 actatagcaa aaccggagga acctattatc agaagatcca ttttgaagtc gtaagcggag
     6661 ctacattgga tatgggagga tcgattattg ataatggttc ttcgggcaat ttcacattaa
     6721 attcaggggg atgcctgaaa accgcacatg tccagggaat atccagcacc ggcgcatccg
     6781 gttgtataca gctcacaggt tcccgtacct ttaatgccgg cgccagttat attttttata
     6841 agagtggcag ccaggctaca gggactggtc tgccggcaac gcttagttca gtcctcacga
     6901 ttggaacaat ccataatgcc acaagcctta cacttaccaa ttctcctgta accattaacg
     6961 gaacgcttgt tcttgtaagt gattctgcaa tcaacagtgc gatcgcctcc ggcaccataa
     7021 gttatggaag caatgggaca ctcgaatacc agggtgacac ggcgcaagta acgacagaca
     7081 tagagtttcc ttcgacttca ggtccttata atttaaaaat taacaatgca tatggcgttt
     7141 cccttcatga agaccggacc ataaacggca cgatttacct ttcaataggg caatttctta
     7201 tcggggctca tacactgaca attaatggaa ccctgtcaca aacaatgggt gtattgaagg
     7261 ggggattgag ctccaatatc atcgtcggtg gaacaggaaa ccttcttacc ctgcctcccg
     7321 ttatgcttaa cgatcttact ctcaaccgac cggctggtat gaccggtacc ggactcgata
     7381 cagtgtacgg cagatttaca ctgacccagg gaacactttg gcccgcgggt gttctggttt
     7441 atggtcctga agcgacactg aaatacaatg gatcaagtgc ccaaactaca accagtgcgg
     7501 aatttcctga gaactacggg ccctgtaacc tcgaaaacag taatcctatc cggttagatt
     7561 tacattcctc aaggattata caggggaacc tgcaattgaa tagcggaata ttttctatcg
     7621 ggtcaaatac actgacgatc aacggtctga taaccggacc aggaaccttg ctcggtggta
     7681 gttcttcgga tattgtcttt ggtggaagcg gacctgcgac tactttacca ggaataaccc
     7741 tatccgacct gaccatcgat cgggccagcg gcattaatat gggaggcacg gtgaccataa
     7801 ccaattccct caatttactg gaaggtgatt tctcgatcgg atttaacacc cttatactga
     7861 atgatgaact taactatgat ggcggcaatt taacgggtag tctgagttcc agactaatca
     7921 ttgcaggcca gggagatccg gtcagccttg atgccatcac ccttaacgag ttgaacctga
     7981 gcaggccttc aatcctttat ttggaggata atatattagt ggaaggcatt ctaatccttt
     8041 cagcaggaaa tatttaccgg caggggcata cactctacgg ttctgatgca accctgattt
     8101 actacggcgc cacagcgcag gctacatcgg gtgaagaatg gccatggaat gatggacctc
     8161 atcatatcat cattcaaaac tcccagggtg tttacctgga ttccagccgg aaagtgaccg
     8221 gggacctgac actggcaaaa ggcgatctgc acatcggcgg gaatacgctt attttggagg
     8281 ggataataaa tccggttatg ggaaacctgt cgggagggac cgaatcagcg ctggaaatag
     8341 aagggaatgg agatggaacg caaatccctt cggttgactt gcgaaaactt aaaattaaca
     8401 ggcaaaccgg ggcctggctg gcgggagtat gcaccatcta cgactcattg cacctggtcg
     8461 ccggaaacct gatggtgaac aatcagacgc tgatcctgca aggcgggccg gtaacaggag
     8521 atccgaataa cctgatcacc gaacccgttt ctaacctggt attcgcggga gatcacacag
     8581 gagtgtctat cccggaaagc attaccgacc ttaattacct ggccgtttca aatccaaacg
     8641 gggtggtatt acagagtgat atattgctta attcgtcatg tgatgtgacc ggttacatga
     8701 tttgcggaga gcattcctta tccggcaatg cgggtgtcaa tattttgccc ggtgcccgtt
     8761 ttgcgacggc tcatccggcg gggatctcgg gaaccatcgg cgtcgctctg ccatcattcc
     8821 tgtcatcggg tgcagattat gaattcaacg gcaattatcc acaggaaaca aatttcctgg
     8881 agacctcaac ccccggtttg ataagaaatc tcattgtgaa tactgctgaa ggcattcccc
     8941 tggtgctgac cgggaatatc accaccagtg agggtgttca tgtgagtagc ggttctattt
     9001 tgactattcc cgatgaaata actcttactg ccgggaatcc tgaatgaata gagttttatt
     9061 caaaaacagg acttttccgg agcaactatt ccatttccgg gtgtttgatt aaatgtttta
     9121 aaaaaacatt ttttgcatgt tgaattatat atcaacctaa tttatacttt tacgtgccca
     9181 aaaacagccc cctgactgta aatgagtatc cttcataagt ttttccttaa cccgataaac
     9241 ctatgaatgt caacgcataa caaataacag caataaattt ccatgttatg ttgaataaaa
     9301 ttatctcttt tcttcttctc attttcatgg taccgctaat ttcattagcc gaatggatac
     9361 cgttgaatag ttcaaattct gctgatcccg caacgccaaa attgacgctt ctccatcaaa
     9421 atgcccggga gacaatcatt aaaattgaac tctccggttt tgagatgaac gattttatgg
     9481 ttggaaggac gacttaccag gctgtgaata tcctttcaga agcgcttacc cacacacccg
     9541 gacagccgga actgccctat gtggctacta tacttgccat tcctgaccag gcttcggttc
     9601 gggtcgaagt tgtggaaaca ggccaggaaa gcacattcag gaatgtttac ctgccgccgg
     9661 caaggaaaag ctggtacgaa ggcgatacgg agccctcata t
//
LOCUS       DAOQXS010000033         9605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7593_length_9605_cov_4.004817, whole genome shotgun sequence.
ACCESSION   DAOQXS010000033 DAOQXS010000000
VERSION     DAOQXS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_9605_cov_4.004817"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..958
                     /gene="sufC"
                     /locus_tag="PKM34_01845"
     CDS             203..958
                     /gene="sufC"
                     /locus_tag="PKM34_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNQ82353.1"
                     /translation="MLLQLKELHVSIKGKEIIHGLNLEVKAGEVHAIMGPNGTGKSTL
                     ALAIAGKEGYEITAGEVIFNGLVINEMPPEERARLGIFLGFQYPVEIPGVSLTNFMRT
                     AINEVREYRGLPPMPAGEFLKMMEEKKQLVEIQSKLTNRSVNEGFSGGEKKKNEIFQM
                     AMLEPMLAVLDETDSGLDIDALKIVANGVNMLRRPDNATIVITHYQRLLTYIVPDFVH
                     VIYNGKIVRSGGKELALELEEKGYDWIKKELNV"
     gene            1025..2437
                     /gene="sufD"
                     /locus_tag="PKM34_01850"
     CDS             1025..2437
                     /gene="sufD"
                     /locus_tag="PKM34_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNQ82354.1"
                     /translation="MKTTLHNGSFKENLLSLYEGNEEYIARNDSAYSSTIRSKGFVHF
                     QNLNLPDRKNELWKNTDLSEVLKNDYIKYLNRAETGQDLNFMFTCEIHNFKTDQVSFL
                     NGWHIKAGSSLPDLPAGVIICSISEAFRFYPDLAEKHYGRYAEAGKDIFLAMNTAFTQ
                     DGLFVYVPDNIILEKPVQMISIINHNENLLIQNRNLVILGKNSRMTLVMCDDSTNQQA
                     SFNNSVTEVFLDEGSHFEHYKLQNLNNSSTLLNSTWFYQEAGSRLDTYAITLNGGLIR
                     NYSNVKLNGSGADAHINGLYLVDRKQHVDNRVFVEHAVADCTSKELFKGILDDEASAV
                     FNGHVLVKKDAQRTNAYQQNRNILLTDKATVNTRPFLEIYADDVKCSHGATVGQLDSE
                     ALFYLRSRGICMASARLLLMYAFAAEVISGMTLQALKDRIDEMIRQRLRGELHICQTC
                     VLHCANQDKEIHFDIDMSKI"
     gene            2523..3743
                     /locus_tag="PKM34_01855"
     CDS             2523..3743
                     /locus_tag="PKM34_01855"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNQ82355.1"
                     /translation="MSFDILHIRHDFPILDVTVHDKQLIYFDNAATTHKPRQVVDRIV
                     AYYKKENCNIHRGVHYLSTMATGAYENARKTVKDFINAEHPHEVIFTKGATEAINLVA
                     QTFGRKNIHQGDEIILSEMEHHSNLVPWQMLAEQKGAVLKVIPVDEQGELEVQHLKSM
                     ITHRTKLVALAHVSNVLGTINPVHRVITLAHEQGIPVLLDGAQAVPHLEVDVKELNCD
                     FYAFSAHKLYGPMGVGVLYGKEKYLEEMPPYQGGGEMVDQVTFQKTTYNKLPYKFEAG
                     TPNVSGVLGFEAALKYILDLDLKKIHAYERELLDYTTLELLKMDAVKIFGNAENKTAV
                     ISYNIGKIHPYDAGTIYDKLGIAVRTGHHCAQPLIDRFGVPGMIRASLGLYNTIEEID
                     RFIEATRQVRRMLA"
     gene            3922..4311
                     /locus_tag="PKM34_01860"
     CDS             3922..4311
                     /locus_tag="PKM34_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ82356.1"
                     /translation="MGAFTSFEEMEVWKKSIHLTRMVYQITIQPAFAKDYYLVNQIRK
                     SAVSIASNIAEGFERDGNREFINFLYIAKGSCGELRCQLYIALELQYMTQESFQEIYN
                     FAKEISVSINKLIKYLQSSDYKGIKYK"
     gene            4487..4906
                     /locus_tag="PKM34_01865"
     CDS             4487..4906
                     /locus_tag="PKM34_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNQ82357.1"
                     /translation="MNITEIEANIVAEFSAFDDWMDRYNYLIELGKDLKVIDEKYKVQ
                     NNLISGCQSRVWLHAEFRDGKIYFSADSDAVITKGLISLMIRVLDGQTPDDILSAELG
                     FLDQIGLKEHLSPTRSNGLTSMIKQMKLYALAFKTKS"
     gene            5099..5425
                     /locus_tag="PKM34_01870"
     CDS             5099..5425
                     /locus_tag="PKM34_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNQ82358.1"
                     /translation="MTDINIVKKDLEEKIIAALKEVYDPEIPVNIYEIGLIYKIRIDD
                     DFLVHIVMTLTTPNCPVAESLPEEVKQKVLAIKGVNDVDLELTFEPPWDMEMLSDEAK
                     LELGIL"
     gene            5555..7366
                     /locus_tag="PKM34_01875"
     CDS             5555..7366
                     /locus_tag="PKM34_01875"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNQ82359.1"
                     /translation="MNLHAVPGPILSSINSPADLKKLPEEQLPLLCKEIRAFLIEETS
                     QNPGHLGSSLGAVELAVAIHYVFETPYDKLIWDVGHQAYAHKILTGRRDLFSTNRTLG
                     GISGFPKMSESEYDAFGTGHSSTSLSAALGMAVASALQGDPERMHISVIGDAALAGGM
                     AMEALNHAGVFKANLLVVLNDNGISIDPNVGAFKDYLARIADGQPVLHTRSNFFEAFN
                     FQYFGPVDGHDVIQLVNRLREIRYIPGPKLLHTLTTKGKGFRKAEEEQTVYHAPGYFN
                     KVTGELIEKESAGGIPPKYQDVFGRTIIELARQNEKIVGITPAMPTGSSLVMMMAEMP
                     DRAFDVGIAEQHAVTFAAGMAARGMIPFCNIYSSFMQRAYDQVIHDVALQKLPVVFCL
                     DRSGLVGEDGATHHGAFDLAYFRAIPGLTICSPMNEEELRNMMFTAQLPGAGPTVIRY
                     PRGRGAMVDWQKPFRELIRGTGRRIREGNDVAILSIGHIGNEAEKAAAMLEKDNISVA
                     HYDMRFLKPPDEALLHEVFRNFSKIITVEDGTITGGLGSAVLEFMADHQYNAQVTRLG
                     IPDRFIEQGTVGELQRECGFDANGIIRSSKFKVQSSK"
     gene            7435..8223
                     /locus_tag="PKM34_01880"
     CDS             7435..8223
                     /locus_tag="PKM34_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ82360.1"
                     /translation="MNWIEIAVLIFSGLAVGFINTLAGGGSVISLSVLMLMGLPAPVA
                     NGTNRIAVTIQTLTAATVFKKKKVLDLKKGTILGIPSAIGSLLGAYIAVDINEATFEK
                     AFAVIMLIMLVFVFYNPDKYIYGKQHLVDKKVTPLQWLIFFVIGIYGGFVHVGIGYFL
                     LAALVLNAGYDLVRANAVKVLIVLMYMPFSFLVFLWMSDINWTYGLVMMIGSVAGAYI
                     ASHLAVNKGIVFVKWSIVVVTVFMAGHFLGIYDIKEMFGVMLGK"
     gene            8423..9373
                     /locus_tag="PKM34_01885"
     CDS             8423..9373
                     /locus_tag="PKM34_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNQ82361.1"
                     /translation="MKYMIFLLIPFLVVACSIKNADRPDYVLVIHGGAGVMEKKDFAP
                     ELEKAYLDKLQQALDTGEAILKAGGTSLEAVTAAVVMLENSPLFNAGKGSVFSETGEN
                     EMDASVMNGKDLTAGAVAGVRTIKNPVLAARKVMEESKHVLLVRDGAEKFAKEHGVEM
                     ADPSYFFTRKRWDALMKAQKHGTVGAVALDKAGNLAAATSTGGMTNKMTGRVGDTPVI
                     GAGNYASNKTCAVSGTGHGEYFIRYAVGHDISALMEYKGLSLDEAANEVIFNKLLPAG
                     GTGGVIAVDKDGNYTMTFNTSGMFRGVVTGDGYRETAIFR"
BASE COUNT         2558 a         2382 c         2468 g         2197 t
ORIGIN      
        1 aaggctatca gcctgatcgt gaacggctat gctaaagagg tgctgaacca tctgccgatg
       61 gaattcgcgg tggaagcgca gaaactgctg agcatatcgc ttgaagggag cgtgggataa
      121 gaagcagccc cctcccttac tccctccccc aattggggga gggacggggt gggagctaac
      181 tttgaacttt gaactaaata taatgttact acaactaaaa gaactgcacg tttcgataaa
      241 gggaaaagag ataattcacg ggttgaacct ggaggtcaag gccggggagg tacatgccat
      301 catggggccg aacggaacgg gaaaaagcac gctggcttta gctattgccg gcaaagaagg
      361 atacgagatc accgccgggg aggtcatctt taacgggctg gtcatcaacg aaatgccgcc
      421 tgaagaacgg gccaggctgg gtatctttct cggtttccag tacccggtgg agatcccggg
      481 cgtcagcctg accaacttca tgcggacagc catcaacgaa gtgcgcgaat accgcgggct
      541 tcctccgatg ccggccgggg aattcctgaa aatgatggaa gagaagaaac aactggtcga
      601 gatccagtcg aagctgacca accgctcggt caacgaaggt ttctcaggcg gtgagaagaa
      661 aaagaacgag atcttccaga tggcaatgct ggaaccaatg ttggctgtgc tcgatgaaac
      721 cgattcggga ctcgatatcg atgcgctgaa gatcgtggct aacggcgtca atatgctccg
      781 gcggcccgac aatgcaacga tcgtcatcac ccattaccag cggctgctga cctacatcgt
      841 ccccgatttt gtacatgtga tctacaacgg caagatcgtc cgttcgggcg gaaaagagct
      901 ggcgctcgag ctggaagaaa agggatatga ctggattaaa aaagaattaa atgtctgaat
      961 aaccccaccc cctgcccctc ccctgatatc aggggagggg ttggagaggg gtccataaaa
     1021 tataatgaaa acaacactgc ataacggatc gttcaaggaa aaccttctca gcctgtatga
     1081 aggaaatgaa gaatatattg cccggaatga ttcagcctat tcatcaacta tccgctcaaa
     1141 gggtttcgtt cattttcaaa atctgaacct tcccgaccgg aaaaatgaac tctggaaaaa
     1201 caccgacctt tctgaagtcc ttaagaatga ctacattaag tatctgaacc gtgccgaaac
     1261 gggacaggac ctgaatttca tgttcacctg cgagatccat aatttcaaaa ccgaccaggt
     1321 ctctttcctt aacggatggc atataaaagc gggaagctca ctgcccgacc ttccggccgg
     1381 ggtgatcata tgcagtatca gcgaagcatt caggttttac ccggatttgg cagagaagca
     1441 ttacggccgt tacgccgaag ccggcaagga tattttcctg gccatgaata ccgctttcac
     1501 acaggacggg ctgtttgtct atgtccccga caacatcata ctggagaagc ccgtccagat
     1561 gatcagcatc atcaatcaca acgaaaatct cctcattcaa aaccgcaacc tggtcattct
     1621 gggcaaaaac agccggatga cgctggtcat gtgcgatgac tcaaccaacc agcaagccag
     1681 tttcaacaac tcggtcacgg aggtctttct ggatgaagga tctcatttcg agcattataa
     1741 gctgcaaaat ctgaataata gctcgacctt gctgaactcg acgtggtttt accaggaagc
     1801 cgggagccgg ctcgacacgt atgccattac tttgaacggc ggattgatcc gcaactattc
     1861 gaatgtgaag cttaacggca gcggcgccga tgcgcatatc aacggccttt acctggtcga
     1921 ccggaagcag cacgttgata accgggtatt tgtcgaacac gctgttgctg attgcaccag
     1981 taaagaactt ttcaaaggca tcctcgacga tgaggcttcg gccgtgttca acgggcatgt
     2041 cctcgttaaa aaagacgccc agcggaccaa tgcctaccag cagaaccgga acatcctgct
     2101 tacggataaa gcgacggtta atacccggcc gttcctcgag atctatgccg acgacgtcaa
     2161 atgcagccat ggagccaccg ttgggcagct cgacagcgaa gcgctgtttt accttcgttc
     2221 gcgtggcata tgcatggcca gtgcccgttt gctgctgatg tacgcttttg ctgccgaggt
     2281 gatctctggc atgacgctgc aggcgttgaa ggaccgcatc gatgaaatga tccgccagcg
     2341 gctgcgtggt gagctccaca tctgccagac gtgtgttctg cattgtgcca accaggataa
     2401 ggaaatccat ttcgatatcg atatgagtaa aatctgatga tccttttact tatcaacagc
     2461 ccacggtttt cggaacaatt ttacttcaat tcaactaatt ttgccgccaa aataaaacaa
     2521 aggtgagttt cgacattctg catatacggc acgattttcc catcctcgat gtgacggttc
     2581 acgataaaca gctgatttat ttcgataatg cggcaactac ccacaaaccc cgccaggtcg
     2641 tcgaccggat agtggcctat tacaagaaag agaactgtaa tatccaccgg ggagtacatt
     2701 acctgagcac catggctacc ggggcctatg aaaatgcccg aaaaacggta aaagatttta
     2761 tcaacgccga acatccgcat gaagtgatct ttaccaaagg ggcgacggag gcgatcaacc
     2821 ttgtggccca gactttcggc agaaaaaata ttcaccaggg cgatgagatc atcctttcgg
     2881 agatggagca tcattccaac ctggtaccct ggcagatgct ggccgagcaa aaaggggcgg
     2941 tattgaaagt tatcccggtc gatgagcagg gcgaactgga agtgcagcac ctgaaatcga
     3001 tgatcaccca ccggacgaaa ctggtcgccc tggcccacgt ttccaacgtc ctgggcacca
     3061 ttaacccggt ccaccgggtg attacgctgg cccacgagca gggcataccg gtccttctcg
     3121 acggcgcgca ggctgtgccg cacctcgaag tcgatgtcaa ggagctgaac tgcgactttt
     3181 acgctttctc ggcacacaaa ctctacggcc cgatgggcgt cggggtgctt tacggaaaag
     3241 aaaaatacct ggaggagatg cctccgtacc agggtggcgg tgagatggtc gaccaggtga
     3301 catttcagaa aacgacgtac aacaagcttc cgtacaaatt cgaggccggc acgcccaacg
     3361 tttccggcgt cctgggattc gaggccgctc ttaaatacat cctcgacctc gacctcaaga
     3421 aaattcacgc ttacgaaagg gaattgctcg attacacgac actggagctg cttaaaatgg
     3481 atgcggtgaa gattttcggc aatgctgaaa ataaaacggc agtcatttct tacaatatcg
     3541 gcaaaataca tccctacgac gccggcacga tctacgacaa actcggcatc gccgtccgca
     3601 ccgggcatca ctgcgcccag ccgctgatcg accgattcgg tgttcccggc atgatccgcg
     3661 cctccctggg gctttacaac accatcgaag agatcgaccg gttcatcgaa gcgaccaggc
     3721 aggtgagacg aatgctagct taaggttgcg ggtttccaaa gttccagtgt tccattgttc
     3781 caaagttcca gtgttccagt gttccaaagt tccagcgttc caaagttcca gagttcatat
     3841 gtgagactgt ttagattaaa gttatcaaca accgggtatt ttttactaaa aagccactct
     3901 aataggataa ataatttcag tatgggtgct tttacaagtt ttgaagagat ggaggtctgg
     3961 aaaaaatcta tccatcttac acgaatggtt tatcaaatta ccattcaacc tgcttttgca
     4021 aaagactatt acctggtgaa tcagataagg aagtctgctg tatcaattgc ttcaaatatt
     4081 gcagaagggt ttgaaagaga tggaaataga gaatttatca attttcttta cattgccaaa
     4141 ggatcgtgtg gagaattgcg ttgtcaactt tatattgctt tagaacttca atatatgacc
     4201 caagaaagct ttcaggaaat atataatttt gcaaaagaaa tcagcgtatc aataaataaa
     4261 ttgataaaat acttgcagag ttctgattat aagggtatta aatataaata aacttgcccg
     4321 aactaaacag actttaaaac attggaacac tggaactttg gaacactgga acactggaac
     4381 actggaacat tggaacactg gaacattgga acactggaac actggaacac tggaactttg
     4441 gaacaatgga acactggaac tttggaaccc ggaaccctgg aacaacatga acataaccga
     4501 gatcgaagca aacatcgtag ccgagttcag cgctttcgac gactggatgg accggtacaa
     4561 ttacctgatc gaactgggga aagacctcaa ggtgatcgat gagaagtata aggttcagaa
     4621 caacctgatc tccgggtgcc agtcgagggt gtggctgcac gcagaattcc gcgacggaaa
     4681 gatatatttc tctgccgaca gcgatgccgt gatcaccaaa ggattgatca gcctgatgat
     4741 ccgggtgctc gacgggcaaa cgccggatga tatcctcagc gcggagcttg gctttctgga
     4801 tcagatcggg ttgaaggagc acctctcccc cacccgctcc aacgggctga cctcgatgat
     4861 aaagcaaatg aaactctatg cacttgcgtt taaaactaag tcataatgaa tgtatttcgt
     4921 gctccaacgg gtaaacccgt tggctatggg tactatttta taaccgatgg gtttacccgt
     4981 cggagcagga acgattgcag cccgacgact gaagccgtcg gttcagggag gattaaaaaa
     5041 tcataaccga tgggtttacc ttcggactac aaatagacca ggttaagcag aaaaaaccat
     5101 gacagatata aatattgtaa aaaaagacct cgaagaaaag atcattgccg ctctgaaaga
     5161 ggtctatgat cctgagatcc ccgtgaatat atacgaaatc ggattgatct acaagatccg
     5221 gatcgatgac gactttctcg ttcatatcgt tatgaccctc accacaccca actgccccgt
     5281 tgcggaaagc cttccggaag aggtaaagca gaaggtactg gcaattaagg gggtaaatga
     5341 cgtagatctt gaactgactt tcgaaccgcc ctgggacatg gagatgctca gtgatgaggc
     5401 aaagctggaa ctggggatac tttagttgga cagttggaca gcatgtcctg agggcgaagc
     5461 ccgaaggatc gcagttggac agttggacag taacccttac aacctttacc agctaaatta
     5521 ctacctttga actttgaact ttgaactttg aactttgaac ttgcacgctg ttcccggtcc
     5581 tatattgtct tccataaact ctccggcaga tcttaaaaag cttccggaag agcagttgcc
     5641 tctgctttgc aaagaaatac gcgcatttct tattgaagag acctcccaga atccggggca
     5701 cctcggttcc agcttaggcg cagtggaact ggcggtagcc atccactatg tatttgaaac
     5761 gccgtacgac aagctgatct gggacgtggg tcaccaggcc tatgcccaca aaatattaac
     5821 cggaaggcgt gatcttttca gcacaaaccg aacacttggc gggatcagcg gattcccgaa
     5881 aatgagcgaa agcgaatacg atgctttcgg aacgggccat tcctcaacct ctttatcggc
     5941 tgcgctgggt atggctgttg cctccgcgtt acagggcgat ccggaacgga tgcacatttc
     6001 cgtgatcggc gatgcggcgc tggccggagg gatggcaatg gaagcgctga accatgccgg
     6061 cgtgttcaaa gcgaacctgc tggtggtttt gaacgataac ggcatttcga tcgatccgaa
     6121 tgtcggtgca tttaaagact acctggcccg gatcgcggat gggcagcctg tcctgcatac
     6181 gcgcagcaat ttcttcgaag cgttcaactt tcagtacttc ggcccggttg atggccatga
     6241 tgtgatccag ctcgtgaaca ggctgcggga gatcaggtac attccggggc caaagctgct
     6301 ccacaccctg acgacaaagg gcaaaggatt ccggaaagcc gaagaggagc aaaccgttta
     6361 tcatgcgccc ggatatttca ataaggtcac cggggaactg atagaaaaag aatcggccgg
     6421 gggaatcccg ccgaagtacc aggacgtttt cggccgcaca atcattgaat tagccaggca
     6481 gaacgaaaag atcgtaggga tcacgccagc catgcccacc ggctcttcgc tggtgatgat
     6541 gatggccgag atgcccgacc gggcctttga cgtggggatc gccgagcagc acgccgtaac
     6601 attcgcagcc ggcatggccg cccgggggat gatcccgttc tgcaacattt attcaagctt
     6661 catgcagcgg gcctacgacc aggtgatcca cgacgtggca ttgcaaaagc tgccggtggt
     6721 attctgcctc gaccgcagcg ggctcgtcgg tgaagacggt gccacgcacc atggcgcttt
     6781 cgatcttgcg tatttccggg ccataccggg cctgaccatc tgctccccca tgaacgagga
     6841 ggagttgcgc aacatgatgt ttactgccca gctgccgggc gccgggccga cggtgatccg
     6901 ctatccgcgg ggccgggggg caatggtcga ctggcagaaa ccattccggg aactgatccg
     6961 cggcacaggc cggcgaatcc gcgaagggaa tgatgtggcg attctgtcca ttggccatat
     7021 cggcaatgaa gcagaaaaag ccgcagccat gcttgaaaaa gataacatct ctgtggcgca
     7081 ttacgacatg agattcctga agccgccgga cgaagcgctg ttacacgaag tgttcagaaa
     7141 tttcagcaag atcatcactg tcgaagacgg gaccatcacc ggcggactgg gcagtgccgt
     7201 gctggaattc atggcagatc atcaatacaa tgcgcaggtg acgaggctcg gcatccccga
     7261 ccggttcatc gaacagggaa cggttggcga attgcagcgg gagtgcgggt ttgatgccaa
     7321 tggaatcatt cgtagttcaa agttcaaagt tcaaagttca aagtgagtcg ggcagctacc
     7381 acccttacca cccctattca gaaattgata cctttgcact ttgaactttg aactttgaac
     7441 tggatagaaa ttgccgtact gatcttctcc ggcctggcgg tcggattcat taacacgctg
     7501 gcgggcggcg gatcggtgat ctccctgtcg gtactgatgc tgatgggatt gcctgcgccg
     7561 gtggccaacg gcaccaaccg gatcgccgtt accattcaga ccttgacggc cgccaccgtt
     7621 ttcaaaaaga aaaaagtcct cgatttaaaa aaagggacga ttctcgggat tccctccgca
     7681 atcggctctt tactcggtgc ttacattgcg gtggatatca atgaggccac ctttgaaaaa
     7741 gctttcgccg tgatcatgct gatcatgctg gtttttgtat tttataatcc ggataaatat
     7801 atttacggca aacaacacct tgtcgataaa aaagtaaccc cgctgcagtg gctgatattc
     7861 tttgtaatag gtatttacgg tggtttcgtc catgtcggga taggttattt cctcctggcc
     7921 gcccttgtgc tcaacgccgg ctacgacctg gtgcgtgcca atgcagtaaa ggtgctgatc
     7981 gtattgatgt acatgccatt ctctttcctc gtcttcctct ggatgagcga tatcaactgg
     8041 acctacggcc tggtgatgat gatcggatcg gtggcagggg cttatattgc ctcgcacctg
     8101 gctgtaaata aagggatcgt gttcgtcaaa tggtcgatcg tggtagtcac cgtttttatg
     8161 gcagggcatt tcctggggat ctacgatatt aaggagatgt tcggggtgat gctggggaag
     8221 tgagtcattg gctcccttcg gtcgcgtcat ttactcgctg cgctcgtgtc ataagctcac
     8281 tgcgttcacg tcatttactc gctgcgctcg tgtcataagg ttgtcagaaa ttgtaccttt
     8341 atatctttat tacctgtggc ccaaaatgat ccgtgttaat ccgcataatc cgtgtcatcc
     8401 gcgtgccatt aaaaagatca ctatgaaata catgattttc cttttaattc cttttctcgt
     8461 cgttgcatgt tcgataaaaa atgctgatcg tcctgattat gtcctggtca tacacggggg
     8521 tgcaggtgta atggaaaaga aagactttgc cccggagctg gaaaaagcat atctcgacaa
     8581 actgcagcaa gcgctcgata ccggcgaagc gatcctcaaa gcaggaggaa cttcacttga
     8641 ggcagttaca gcggctgtcg tcatgcttga aaactccccg ctttttaatg ccggcaaggg
     8701 atctgtcttt tcagagacag gcgagaatga gatggacgct tcggtcatga acggcaagga
     8761 tctcaccgcc ggcgccgttg caggggtccg gaccatcaaa aacccggtcc tggctgcccg
     8821 gaaagtgatg gaagagtcga aacatgtctt actggtccgg gacggcgcgg agaagttcgc
     8881 aaaagagcat ggcgttgaga tggccgatcc ctcctatttc ttcacccgga aaagatggga
     8941 tgcgctgatg aaagctcaga aacacgggac tgtcggcgct gtggcgcttg ataaagccgg
     9001 caatctggct gctgccacct ccaccggtgg catgaccaac aaaatgaccg gccgggtggg
     9061 cgacacgccg gtcatcgggg cgggcaatta cgccagcaat aaaacctgcg ccgtttcagg
     9121 gacaggacac ggcgaatact ttatccggta tgctgtcggc catgacatct ccgccctgat
     9181 ggaatataaa ggactgagcc tggatgaggc agccaacgaa gtcattttca acaagcttct
     9241 gcccgccggc ggcacgggag gagttatcgc cgtggataaa gacggcaatt acacgatgac
     9301 attcaacacc agcgggatgt tccggggggt ggtgacgggc gatggatacc gggaaacagc
     9361 gattttcaga tagaaacaat tccaggttcc aagtaccatc ccgagtaatc gggaccaaat
     9421 aaattccaaa atcaaaaatc caaaataact caataactcc aactcacaat ctcaacactc
     9481 cgaactcaac acttaatact cagtgctaat atctcggctc ggctccccag aaattctgat
     9541 aatcgtctgc atcttccccg tatgtttcga cttttttatc gctgctgatc ttataggtgc
     9601 atttc
//
LOCUS       DAOQXS010000034         9547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7656_length_9547_cov_3.524652, whole genome shotgun sequence.
ACCESSION   DAOQXS010000034 DAOQXS010000000
VERSION     DAOQXS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7656_length_9547_cov_3.524652"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKM34_01890"
     CDS             complement(<1..184)
                     /locus_tag="PKM34_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82362.1"
                     /translation="MPGPVGGVPHFGDAVAQVDLDRFGGVKIFPGTEESHEKVRRFDQ
                     VGGIIEAVEFDGFAGVA"
     gene            155..955
                     /locus_tag="PKM34_01895"
     CDS             155..955
                     /locus_tag="PKM34_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNQ82363.1"
                     /translation="MWNAANWAWHTNGEKKLYWHWSPNNGFDMNFPIWGWNECLITYI
                     MAASSPFHAISRDVYEGTWTGSSGFKNGKKYYGIELPLGNYEGGGPLFFEHYTFMGID
                     PNGLKDDMGIDYLEQVRNHTLINRAYCIENPKNYKGYGGNCWGLTAGDSYKGYVAHCP
                     DSDFGVIQPTAALSSFPYTPEYSMHALKHFYYDLGDKIWSEYGFTDGFSIHHDWYAES
                     HLAIDQGPIVVMIENYRTGLLWKLFMQIPDIQKGLETLGFESPWIKGK"
     gene            1082..3283
                     /locus_tag="PKM34_01900"
     CDS             1082..3283
                     /locus_tag="PKM34_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNQ82364.1"
                     /translation="MIKLPSLLLKPLTTLTILTTFTTFTTLTTLTTFSQEYRYNYCFF
                     TNSPMPGDWYFSMSAAKEGSSVKNSSGKLPVNDQVFHSPGNSLELFCESEPGGNWNVL
                     LLHSGKRGMDFFKKSDALSIWIYNNSENTSENEVPYIQVMQYDSSLSSAFPLPLTPFR
                     IWQQVIIPFDSLGIKRSSRAETFPGLYLSQNPASSGSIELYLDDIELITTGAASAVVQ
                     LPEITETKGYARHVDIKWNEVTDPNVRLVKIYRQAEDTGPVPVGVQQPYINRYADFTG
                     ESGKKYSYTITFLDAGYHETGPSPAAKAETRALSDDELLTMVQEACFRYYWEGAEPNS
                     GMARENIPGRRNMIATGASGFGIMALLVGTERKFITRNESIERFLKILNFLEKADRFH
                     GAYGHFMNGTTGEVVPFFGQRDNGADLVESAFLLQGLLAARQYFDGKTADEQMIRDKI
                     TAIWEGLDWSWFRKEPDSRFLFWHWSPDRGWVINHRLIGWNETMVVYLLAIASPTHPV
                     PGSMYFTGFASREPLAMKYRYDWGGSMDGALYINGNHYSGIKLDVGVNNGGPLFFTHY
                     SYMGYDPHFITDQYTNYFNNNTSIAKINYNYCVSNPKRYKGYGENAWGLTACDGPAGY
                     SADEPIPSRDFGKLAPTGAIASFPYTPVESMKALKNYYLNLGHFLWGEYGFKDAFNLT
                     DNWCSEIYMGLNQAPMTVMIENYRTGLVWKLFMQDPEIREGLKRLSVLKSE"
     gene            complement(3354..4550)
                     /locus_tag="PKM34_01905"
     CDS             complement(3354..4550)
                     /locus_tag="PKM34_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ82365.1"
                     /translation="MSEFSRNQASRADQLVGYVIGLIEGRKGSGLLEQYKVLETDFQP
                     GDILILFDNLFEKKYPMEGIKSASNKLFNILFKTLSPYRRNDYPRNSFLWILQKDNAG
                     IKKILGKARNDIKKINSDADLLALSRLEATFRGAEKFLEHYVVMENIVFPEIEKQWKN
                     HQCLKLMWSFHDDIRRNLHATCEILRAAPFNLKQFNETVSKVYFNISTIVFREEHVLF
                     PILHESFGDELFVKMGDQLREFDLAYADVRKIRASGQMPEERSLTGDMVRLATGELTP
                     DQIDLIFNHLPVDITFVDENNRVKYFSSPKHRIFPRTASVINRRVQDCHPHESVHIVN
                     KIVDSFRSGEKDEAAFWIRMKDKFVLIRYFAVRDKEGNYRGVLEVSQEISGIQAITGE
                     RRLLDW"
     gene            complement(4591..4929)
                     /locus_tag="PKM34_01910"
     CDS             complement(4591..4929)
                     /locus_tag="PKM34_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNQ82366.1"
                     /translation="MEKIIAACGLNCAQCEARIATLADDNDLRAKTAEKWKVQFNAPD
                     IPIEAINCTGCMEPGVKLSHCSECKIRNCAIGKGFTTCAECSILESCELLKMVHQYDP
                     SALENLKMMN"
     gene            complement(4951..5430)
                     /locus_tag="PKM34_01915"
     CDS             complement(4951..5430)
                     /locus_tag="PKM34_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82367.1"
                     /translation="MLLNLIDILRIVIVCFGIFWGYTIGFDNGYDPVAQLHVTIPVVI
                     VAIAGFSGLEGLLFGAKTAENKGFKTDGNYQRQSAIALLSYAAVALVVYFCKWGIKAE
                     LTIFFAFIFFFIFSGINHAVDAFRNKNYKWQNINRPFITFFLTAALIYPIIKVIQDL"
     gene            complement(5467..6102)
                     /locus_tag="PKM34_01920"
     CDS             complement(5467..6102)
                     /locus_tag="PKM34_01920"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HNQ82368.1"
                     /translation="MKPSPREKSSMIFRSALGAMAWIAVILDFILSDELIWNFISYFT
                     IISNTLIAVAMTLTVTVPGSRFGAFFSRSGIQSALALYIIIVGLVYNFNLRNTWAITG
                     FLSIVDYLLHVVIPLAYIVYWLIFIPKRALNWTHSIIWLIFPLVYLGYSLARGAITGW
                     YPYPFLDVAKLGYEKVLLNSAFVTSGFLLFAVLLIFINRSVKLLPKRTRST"
     gene            complement(6099..6533)
                     /locus_tag="PKM34_01925"
     CDS             complement(6099..6533)
                     /locus_tag="PKM34_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNQ82369.1"
                     /translation="MADKNTALTASIKLINNKLHFEGHAGDNAPVSIDYIAPLGDDLG
                     YTSLELLLLSYSSCLGSAVLTFLRRMGKTIRGFEIQAKGIQHTEHPTGFSTIHIEMEL
                     GSPDVLPEDLEKVIALAKEKYCPVWAMIKGNIDIIINYSIHP"
     gene            complement(6642..7052)
                     /gene="rnk"
                     /locus_tag="PKM34_01930"
     CDS             complement(6642..7052)
                     /gene="rnk"
                     /locus_tag="PKM34_01930"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNQ82370.1"
                     /translation="MSKLIINKLDYARIRKSIEDARQFKSISSIDAEKLMNELNSAEI
                     VEPEAIPSNVVTMNSIVKISFLNNDKQIQFQIVYPSKANIKENKISIFSPIATALIGY
                     KVKDEIEWIVPAGLTKIRIDEIIYQPEAAGEYHL"
     gene            complement(7136..8662)
                     /locus_tag="PKM34_01935"
     CDS             complement(7136..8662)
                     /locus_tag="PKM34_01935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ82371.1"
                     /translation="MENLYSGIVTILICIAGYAFAFRQYSRDNVQAALILIMVCGLLL
                     RLYAASDFYLHAWDERYHALVAKNMITQPFRPMLYSDPVLPYDFRNWVGNHVWIHKQP
                     VPLWSMAFSMSVFGVNEIALRLPSVILTTLGIGITFYVAKYLFNQRVGIIAAFLYSIH
                     GLIIELSAGRVATDHIDVFFLFFVQLSVLLAIRFFQSKRLVFNVLCGISIGMAILSKW
                     LPALIVLPIWLLLALDSKKLSRKEIIIDFLVLIAVIAIVSIPWQWYIYSHFPQEARWE
                     SSFNLKHITEGLENHGQPFYYHFEKVMVLYGEIIYLPLLWFFYKSFKHLKKYRRLVLT
                     LWILIPILFFSIARTKMQAYTIFAAPAFFIVTALFWEYLFRYRHRLKYKALTITILFL
                     LLALPVRYSIERVKPFIIRERNPQWTKELKELDKQLPDYRNVVVFNARYPIETMFYTD
                     CMAYETIPDSAALTGLDQRGYSIFIREETDKGSGNRGDGESDILMTGYNVKYCKWSGK
                     "
     gene            complement(8671..>9547)
                     /locus_tag="PKM34_01940"
     CDS             complement(8671..>9547)
                     /locus_tag="PKM34_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ82372.1"
                     /translation="RASPRPDQRIVHCPLSIVHCPLSMAEGKIRKSNAEDRKSTINSR
                     PSLARTGALRKPLQNMFMMKINIVLLLILISVNGFSQTDPFTQDWANLKKYESENRSL
                     KLPADGENRVVFLGNSITEFWKYADSSFFIGKPYLNRGISGQTTPQMLLRFRPDVIDL
                     QPAVVVILAGINDIAENTGPASMESIYGNIISMVQLARANEIKVVICSILPANHFYWR
                     PQIEPAEKVIKLNSLLENYCKQNNLIFLDYYSGMVDSEKGLDKKYTDDGVHPTLAGYK
                     YMEPLVEEAIQKALK"
BASE COUNT         2554 a         2367 c         2224 g         2402 t
ORIGIN      
        1 cggcaacacc ggcaaaacca tcaaattcga ccgcctcgat gatgccgccg acctggtcga
       61 aacgtcttac cttctcatgt gattcctctg tgccagggaa tattttaact ccaccgaacc
      121 gatcgaggtc tacctgcgca accgcatcac caaaatgtgg aacgccgcca actgggcctg
      181 gcataccaat ggcgaaaaga agctctactg gcactggagc cctaacaacg gcttcgacat
      241 gaacttcccc atctggggat ggaacgaatg cctcatcacc tatatcatgg cagcctcctc
      301 ccctttccat gccatctccc gcgacgtgta cgagggcacc tggacaggca gctccggatt
      361 caaaaacggg aaaaaatatt atggaatcga actgcccctg ggtaattacg aaggcggcgg
      421 acccctgttc ttcgaacatt ataccttcat ggggatcgat ccgaacgggc tgaaagacga
      481 tatgggcatc gactacctgg aacaggtacg caaccatacc cttatcaacc gggcttactg
      541 catcgaaaat ccaaaaaact acaagggtta cggcggcaac tgctgggggc tcacggcagg
      601 cgacagttac aaaggatacg tagcccattg tcccgatagc gacttcggcg ttattcagcc
      661 cactgctgcg ctctcatctt tcccttacac ccccgaatat tccatgcatg ccctgaagca
      721 tttctattac gacctcggcg ataagatctg gagtgaatac ggttttaccg atggtttcag
      781 catccatcac gactggtatg ccgagagcca tttggccatc gaccagggcc cgatcgtggt
      841 catgatcgaa aactaccgca ccgggctcct ctggaaactg ttcatgcaga ttcccgatat
      901 tcagaaaggg ttggaaaccc tcgggtttga aagcccctgg atcaagggga agtaggatcc
      961 agagttcctg gttccgggtt ccaatgttcc agagttccag ggcctgtcct gagggcgaag
     1021 cccgaaggtt tcctggtttc ttaaagttag atattgtatt tttggctaat aatccaatgt
     1081 tatgataaag ctaccctctt tactcctcaa accccttacc acccttacca tccttaccac
     1141 ctttaccacc tttaccaccc tcacaaccct caccaccttc tcccaggaat accgctacaa
     1201 ctactgcttc tttaccaaca gcccgatgcc cggcgactgg tatttcagca tgagcgcggc
     1261 taaagaggga tcctccgtga aaaacagctc gggaaagctg cccgtgaacg accaggtctt
     1321 ccattcaccg ggaaactcac tcgagttgtt ttgtgagagt gaacccggtg gaaactggaa
     1381 cgtcctcctg ctccattccg gtaaaagggg aatggatttt ttcaagaaat ctgacgcttt
     1441 atccatatgg atttacaata attctgaaaa tacaagtgaa aatgaagtgc cctatattca
     1501 agtcatgcaa tacgatagca gcctgagttc tgctttcccg cttccgctga cgccttttcg
     1561 catatggcaa caggttatca tcccgttcga cagcctgggc attaaaagat cgtcccgggc
     1621 agagaccttc ccggggttgt acctttcgca aaatcctgcc tcatccggtt caattgagct
     1681 atatctcgac gacatcgaac tgatcaccac cggcgccgcc agcgctgtgg tacaattacc
     1741 ggaaatcacc gaaacaaaag gctatgcccg gcatgtcgat atcaaatgga acgaggttac
     1801 cgatccgaac gttcggttgg taaaaattta ccggcaggcg gaagataccg gacccgtgcc
     1861 ggttggtgtg caacagccct atatcaaccg ttatgcagat tttacgggag aatcgggtaa
     1921 aaagtattcc tatacgatta ccttcctcga tgccggttac catgaaacag gcccctctcc
     1981 tgccgccaaa gcggagaccc gggccctgag cgacgacgaa ctgctgacga tggtgcagga
     2041 agcctgtttc cgctactatt gggaaggggc cgaaccgaac agcggcatgg cgcgcgaaaa
     2101 tattcccgga cgccgcaaca tgatcgcaac gggcgcatcc ggattcggta tcatggcttt
     2161 gctggtcgga acagagcgga aattcatcac ccggaatgaa agtattgagc ggttccttaa
     2221 aatcctcaat ttcctcgaaa aagccgaccg gtttcacggc gcttatggtc acttcatgaa
     2281 cggcacaacg ggtgaagtgg tccccttctt cggtcagcgc gacaacggcg ccgacctggt
     2341 cgagtcggcc ttcctgctgc agggattgct cgcggcaagg caatatttcg acggaaagac
     2401 tgccgatgaa caaatgatca gggataaaat caccgcaatc tgggaaggcc ttgactggag
     2461 ctggttcagg aaagagcccg acagccggtt tctcttctgg cactggtcgc ccgacagggg
     2521 ttgggtgatc aatcaccggc tgatcggctg gaacgagacg atggtggttt acctgctggc
     2581 catcgcttcg ccaacccacc cggtgccagg cagtatgtat ttcaccggtt tcgccagccg
     2641 ggagccctta gctatgaaat accgctatga ctggggtggt tccatggatg gcgcacttta
     2701 tatcaatggc aaccattact ccggaataaa gctggatgtc ggcgtaaata acggcggccc
     2761 gctcttcttc acccactatt cctacatggg ttacgatcct catttcatta ccgatcaata
     2821 caccaactac ttcaataaca atacatccat agcaaaaatc aattacaatt attgcgtttc
     2881 aaatcctaaa cgttacaaag gatatggcga gaatgcctgg ggactgacgg cctgcgacgg
     2941 cccggccggg tattccgccg atgagcccat cccgtcgagg gatttcggaa agcttgcccc
     3001 caccggcgcc atcgcttcat tcccttatac gcccgttgaa tcgatgaaag ccctgaaaaa
     3061 ttactatctc aacctggggc attttttatg gggcgaatat ggctttaaag atgccttcaa
     3121 cctgaccgac aactggtgtt ccgaaattta tatgggactg aaccaggcgc caatgacggt
     3181 aatgatcgaa aactaccgca ccgggctggt atggaaactt ttcatgcagg atccggagat
     3241 ccgggaaggg ctgaagaggc tgtcggtact gaaaagcgaa tgacattcat tgatggatga
     3301 cggtagtatt tcaaacccgg ttgacgcatg tcatcccgat gcgaagtaag tgatcaccag
     3361 tcgagcagcc ggcgttcgcc tgtaatggcc tggatacctg atatttcctg cgagacttcc
     3421 agcacacccc gatagttccc ctctttatcc ctgaccgcaa aataccggat cagtacgaac
     3481 ttatccttca ttcggatcca gaaagccgct tcgtcctttt cacccgaccg gaaggaatcg
     3541 acaatcttat ttacgatgtg gacgctttca tgcggatgac aatcctgcac gcggcggttg
     3601 atcacgcttg cggtacgggg aaagatccgg tgcttcgggc tggagaagta cttcacccgg
     3661 ttgttctcat ccacgaaggt gatatccacc ggcaaatgat taaagatcag atcgatctgg
     3721 tccggagtta attcgcctgt cgccagccgg accatatcac cggtaagtga tctctcctcc
     3781 ggcatctgcc cggaagccct gattttcctg acatcggcgt aagccaaatc aaactcccgg
     3841 agctgatccc ccatcttaac aaataattca tcaccgaaag attcgtgaag gattggaaag
     3901 agaacgtgct cctccctgaa aacgatcgtg ctgatgttga aatagacttt gctgaccgtt
     3961 tcgttgaact gcttcaggtt gaaaggggcc gcccggagga tttcgcaggt cgcatgcagg
     4021 ttgcgccgga tatcgtcgtg aaacgaccac ataagcttta agcactggtg attcttccat
     4081 tgtttttcaa tttccgggaa cacgatattc tccatcacca catagtgctc caggaacttt
     4141 tcggcaccac ggaatgtcgc ttccaggcgt gaaagtgcaa gcagatccgc gtcgctgttg
     4201 atcttcttaa tgtcattccg cgctttgccc agtatttttt ttataccggc attgtccttt
     4261 tgaagaatcc agaggaagga attgcgcggg taatcattcc gacgataagg cgaaagtgtt
     4321 ttaaacagaa tattgaaaag tttgtttgaa gccgacttga tcccttccat cggatatttc
     4381 ttttcaaaaa gattatcgaa aagaataagg atatcaccgg gctggaaatc cgtttccaga
     4441 actttgtact gctccagcaa accgctccct ttcctgcctt cgatcaggcc gatgacatag
     4501 cctactagct gatccgcacg gctggcttga ttccgggaga attccgacat gttaaatcag
     4561 gttcagattt atccaattgg agactccatc tcaattcatc atcttcagat tttccaaagc
     4621 cgaaggatca tattgatgga ccattttcag cagctcgcag ctttccagta tactgcactc
     4681 ggcacaggtc gtgaaaccct tgccaatggc gcagttcctg attttgcact cggaacaatg
     4741 gctcagttta acgccgggct ccatgcatcc ggtgcaattg atcgcctcaa tcgggatatc
     4801 gggcgcattg aactgcacct tccatttttc ggctgttttt gcacgtaagt cgttatcatc
     4861 ggccagggta gcgatcctgg cctcacactg agcgcaattc agcccgcagg cagcgatgat
     4921 cttttccata aactgttatt ttttaatgtt ctataaatcc tgaataactt ttattatcgg
     4981 ataaatcaat gcagcggtca gaaaaaaagt gatgaacgga cggttgatat tctgccattt
     5041 gtaatttttg ttccggaaag catccacggc atggttgatc ccagaaaaga tgaaaaagaa
     5101 gataaacgca aaaaagatcg tcagttcggc tttgatcccc catttgcaga aatatacaac
     5161 aagtgcaacc gccgcatagg aaagcagggc aatggccgac tgccgctggt aatttccatc
     5221 ggtcttaaag cccttgttct cagctgtttt cgctccgaac agaagacctt ccaaaccgga
     5281 aaagccggca atggccacga taaccaccgg aatagtaaca tgcaactggg caaccgggtc
     5341 atatccattg tcgaacccga tggtatagcc ccagaaaata ccgaagcaga cgatgacaat
     5401 cctgagaata tcgattaagt ttagcagcat aggcgtaaag attctttttt aaaagccgat
     5461 ataaaattat gtcgatcgtg tcctttttgg taacagttta acactcctgt tgataaaaat
     5521 aagcaatacc gcaaacagga ggaacccgga tgtaacgaaa gcgctgttaa gcagcacttt
     5581 ttcatagccc agtttagcca catccaggaa aggataggga taccatccgg tgatggcgcc
     5641 gcgtgccagc gaataaccca ggtaaaccag cgggaagatc agccagatga tcgaatgggt
     5701 ccagtttaag gcccttttcg ggataaatat cagccagtag acaatataag ccagcggaat
     5761 gaccacatgg agcaggtaat cgacgatgct gagaaacccg gtaatggccc acgtatttcg
     5821 caggttgaag ttatagacca gcccgacgat gatgatatat aacgccaggg ccgactggat
     5881 gccggaacgg gagaagaatg cgccgaagcg ggatccgggt accgtcaccg tcagtgtcat
     5941 cgctacggcg ataagggtat tggagatgat ggtaaaatag ctgataaagt tccagatcag
     6001 ctcatccgac aggataaaat cgagtatgac ggcaatccag gccattgcgc cgagcgctga
     6061 ccggaatatc atggaagatt tttcccgggg cgatggtttc atggatggat tgaataattg
     6121 atgatgatat cgatgttccc tttgatcatc gcccacaccg ggcaatattt ttctttcgcc
     6181 agggcgatca ctttctccag gtcttcaggc aaaacatcgg gcgatcccag ttccatttca
     6241 atatgaatag ttgaaaatcc ggtggggtgt tcggtgtgct ggatgccttt ggcctgaatc
     6301 tcaaatcccc ggatcgtttt ccccatcctg cgcaggaatg tcagcacagc gcttccgagg
     6361 caggaggagt agctgagaag cagcaattcc agcgaggtat agcccagatc atcccccagc
     6421 ggggcgatgt aatcgatgga gacgggtgcg ttgtcgccgg catgtccttc aaaatgcagt
     6481 ttgttgttga tcagtttgat cgaagcggtg agcgctgtgt ttttatcagc catatcagtc
     6541 aggtaataat cggcaacaaa tctaaaaata atattccgga aaaaggagac tccatctcga
     6601 tttagtgaga tggagtctca atatgcggtc aggacttctt tctataaatg atactccccg
     6661 gctgcctcgg gttggtaaat gatctcgtcg atcctgatct tggtcagacc ggctggaacg
     6721 atccattcga tctcatcttt gactttatac ccgatcaggg ccgttgcaat gggtgaaaag
     6781 atagatatct tattctcttt gatatttgct ttactgggat aaacgatctg gaactggatc
     6841 tgtttatcat tatttaaaaa gctgatcttc actatcgaat tcatcgttac cacattggaa
     6901 ggaatggctt ccggctccac tatttctgcc gaattcagct cattcatcag tttttccgcg
     6961 tcaatgctgc ttattgattt aaactgcctt gcatcctcga tgctcttcct gatacgggca
     7021 taatccaact tattgattat caatttactc atgattatta ttcatttgat taatatgggg
     7081 ataaagatac ggctaatttt tgatatctgg atctcacgaa gacaagagca tacgactatt
     7141 taccgctcca tttgcaatac ttcacgttat acccggtcat cagaatatca gattcaccgt
     7201 cgccccggtt gcctgatccc ttgtcggttt cttcacgaat aaatatggaa tagcctcttt
     7261 gatccaggcc cgtaagtgct gccgaatcag gaatggtctc ataggccata caatccgtat
     7321 agaacatggt ttctatggga tatcgggcat taaagaccac tacattccta taatccggca
     7381 attgcttgtc taattccttc agttccttcg tccattgcgg atttcgttcc ctgataataa
     7441 aaggcttgac gcgttcgatg gaatacctga ccggaagcgc taataataaa aataatatag
     7501 taatggtcaa agctttgtat tttaaccggt gtctgtaacg aaacaggtat tcccagaata
     7561 atgccgtaac gataaaaaat gccggcgccg caaatatcgt atacgcctgc atttttgtcc
     7621 tggctatgga aaagaacaaa atgggaatca aaatccaaag agtcagaact aatcttctgt
     7681 attttttcag atgtttgaaa gatttataaa aaaaccataa tagcggcagg taaatgatct
     7741 caccgtaaag caccataacc ttctcaaaat gataataaaa aggctgacca tggttttcca
     7801 ggccctcggt gatatgtttc aggttaaaac tgctttccca tcgtgcttcc tgcggaaaat
     7861 gactataaat gtaccattgc cacggtatgg aaactattgc gataacagct attaatacaa
     7921 ggaaatcaat gattatctcc tttctgctta actttttcga atcaagagcc agaagcagcc
     7981 agataggcag gacaatcaac gccggcagcc atttcgataa aatggccatg cctatactta
     8041 ttccgcacag gacattgaaa acaagtcttt tactttgaaa aaaccggatc gccaggagga
     8101 ccgacaattg gacgaagaac aggaaaaaaa catcgatatg gtcggtggca acacggcctg
     8161 ctgacaattc tatgatcaaa ccatggatgg aatagagaaa agcggcgatg atgccaactc
     8221 tttgattgaa aagatatttc gccacataaa aagtgattcc gatgcccagg gtggtcaaaa
     8281 taacggatgg caggcgcagg gcgatttcat tcactccgaa gactgacatg ctgaaagcca
     8341 tcgaccatag cggaacgggc tgtttatgga tccagacatg gtttccgacc caattcctga
     8401 aatcataggg caaaacagga tcactgtaaa gcataggacg aaacggttgg gtgatcatat
     8461 ttttggctac aagggcatga taccgctcgt cccatgcatg taaataaaag tcggaagcgg
     8521 catagagtct cagcaataat ccgcaaacca tgatcaggat caaggctgcc tgaacgttat
     8581 ccctggaata ctgccggaaa gcaaaagcat atccggcaat acagatgaga atagtaacta
     8641 ttccggaata gaggttctcc atggtgacgt ctatttcagg gctttttgaa tcgcttcctc
     8701 caccaacggc tccatatatt tgtaaccggc cagggtcgga tggactccat cgtcggtgta
     8761 ctttttatcc aggccctttt ccgaatcgac catcccggaa taataatcca ggaaaatgag
     8821 gttattctgt ttgcaataat tttccagtaa cgaattcagc tttattactt tttcagccgg
     8881 ttcaatttga ggccgccaat aaaaatgatt ggccggaaga atcgagcaga tcaccacttt
     8941 tatttcgttg gcccgggcaa gctgcaccat cgatataatg tttccataaa tactttccat
     9001 tgaggcaggc cccgtattct ctgctatatc gttgatcccg gcaaggatga caacaactgc
     9061 aggttgcagg tcgatgacgt cagggcggaa acggagtaac atctggggtg ttgtctgccc
     9121 gctgatcccc cggtttagat agggtttccc aatgaaaaat gagctgtcgg catatttcca
     9181 gaattcggta atggaatttc ccaggaatac aacgcggttt tctccatctg ccggaagctt
     9241 caaactccgg ttttcggatt catacttttt caggtttgcc caatcttgag taaacggatc
     9301 tgtctggctg aaaccgttaa cagagatcaa aatcaggagc agtacgatat ttattttcat
     9361 catgaacatg ttttgtaaag gtttgcgcag tgcccccgtt cgggcaaggc tgggccggga
     9421 attgattgtc gattttcgat cttcggcgtt cgattttcga atcttccctt cggccatcga
     9481 caatggacaa tggacaatgg acaatggaca atgaacaatc ctctggtccg gcctgggcga
     9541 tgcgcgt
//
LOCUS       DAOQXS010000035         9481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7730_length_9481_cov_3.512837, whole genome shotgun sequence.
ACCESSION   DAOQXS010000035 DAOQXS010000000
VERSION     DAOQXS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_9481_cov_3.512837"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKM34_01945"
     CDS             complement(<1..281)
                     /locus_tag="PKM34_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor"
                     /protein_id="HNQ82373.1"
                     /translation="MLSRRHLRVKALQALYAFFQSDGQDLVAGEKQLLRSTDKLYELY
                     IWQLSFLVRFADYSLNRIEEAKKKFYPTEEDLNPNTRLVDNKIIAQIDK"
     gene            503..868
                     /locus_tag="PKM34_01950"
     CDS             503..868
                     /locus_tag="PKM34_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNQ82374.1"
                     /translation="MEEFGNKEIRKEEVFSRVIRAGKRTYFFDVKATRSGENYLTITE
                     SKRRFDNEQGKFFYEKHKIFLYKEDFEKFLTGLNQVMHFVETGEKPEGIEDEAFSGEE
                     AHTDFDFDDLGDHSKDTDH"
     gene            1013..1828
                     /locus_tag="PKM34_01955"
     CDS             1013..1828
                     /locus_tag="PKM34_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ82375.1"
                     /translation="MGKVIALANQKGGVGKTTTAINLGASLAVLEYKTLLVDADPQAN
                     TTSGVGFDPKNIKTSVYECIIDDLDPNNILLNTQTPNLDLLPAHIDLVGAEIEMINLP
                     NREKMMRRVVQKVRHKYDFILIDCSPSLGLVTINALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQTRLNPELDIEGILLTMFDQRLRLSKQVAEEVKTHFQQMVFDTIIHRNTR
                     LSEAPSFGETVIMHDATSIGAINYLNLAREILQKNGMTHLSEADKTIDIVNDI"
     gene            1818..2705
                     /locus_tag="PKM34_01960"
     CDS             1818..2705
                     /locus_tag="PKM34_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNQ82376.1"
                     /translation="MTSKKKALGRGLDAILQSPETDITSRDISGDYVAGAVAEIEIGK
                     IEANPFQPRTDFEAESMEELARSIGEQGIIQPLTVRKAGFDRYQLIAGERRLKASKMA
                     GLSTVPCYIRVANDEQMLELALIENIHRKDLNAIEIGISYQRLIEELNLTQEEVSKKV
                     GKDRATISNYIRLLKLPVEVQYALRNDQISMGHARTLIGISDEEKQLKTLLKIVEKGL
                     SVREAEKLAREIQAEPAVKEPALSSLPEHLQEAGLKLHEKIGAAVRIKSNTKGAGSLV
                     IKFGSGNELERILKLLNES"
     gene            2702..3280
                     /locus_tag="PKM34_01965"
     CDS             2702..3280
                     /locus_tag="PKM34_01965"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNQ82377.1"
                     /translation="MNLTFPDKNLILSFLAVLFLILACKTAASQDTTKTRREPAKHSV
                     GKAAIFSAVLPGLGQAYNRKYWKIPVVYAGFGAIGYFVVTNDKQYDTFKEAYLYVANA
                     DTTPIDNPYVTKYSKDQLRSSMEYYRRNRDLSIIIGALWYAVVILEAYADAHFFDYDV
                     SEDLTLKVGPAALPSPERPRQPVPGIHITLKF"
     gene            3284..4006
                     /gene="dapB"
                     /locus_tag="PKM34_01970"
     CDS             3284..4006
                     /gene="dapB"
                     /locus_tag="PKM34_01970"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNQ82378.1"
                     /translation="MKIILSGYGRMGHEVEKAAIARGHRIVARIDTEDDWATCGPLFS
                     SADVVIDFSRPASAVANIRHCFDHHIPVVTGTTGWNKDEPLVRKWCADENQSLFVGSN
                     FSIGVNILFALTGKLSELMNRFDEYNISLEEIHHIHKLDAPSGTAIKLAEMILSRVER
                     KKSWVNQTERTPEELQVISVREGEVPGTHSIVCDSEADRITLRHEAKNRKGFALGAVM
                     AAEWLPGKPGYFDMNDLLGLTL"
     gene            4093..5607
                     /gene="lepB"
                     /locus_tag="PKM34_01975"
     CDS             4093..5607
                     /gene="lepB"
                     /locus_tag="PKM34_01975"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ82379.1"
                     /translation="MNIFLLLFIIFFISSLVGLWKIFIKAGYPGWTVLVPFYNFYIWL
                     KIIKKPLWWYIFLLIPFINVFTILLMIVELLKCFNKFGLGAQALGVLFPFAYLPYLGF
                     TEKEKYIHPDKRKEFKKTWLREWVDAIIFAVIAATIIRTFLIEAYTIPTPSMEKTLLV
                     GDFLFVSKMSYGPRVPMTPLSFPFVHHTLPMTTETKSYLEWIKLPYYRFPGFGKVQRN
                     DAVVFNYPDGDTLSVKFQSNRSYYSMVREYGWSRVNRNSREFGEIISRPVDKRENYIK
                     RCIAIAGDTLQIIDQQVYINGEKAFNPGITEYQYLIKSNISQLNPRLLDELDVTDKVW
                     PVGPGEFVATLTDKAANELRRVKGIESVEVLMEPAGKWDRETFPYSGDYPWNRDNYGP
                     IVMPKAGTSIDLTLKNLPLYERIIKNYELNDLKVTDGRIYINGVESSQYTFKMNYYWM
                     MGDNRHNSADSRYWGMVPEDHIVGKAVFVWFSMDKNKSLFDGKIRWNKLLRTVK"
     gene            5635..6300
                     /locus_tag="PKM34_01980"
     CDS             5635..6300
                     /locus_tag="PKM34_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNQ82380.1"
                     /translation="MKKSSMLTWLLAAVFMLTSFATFAKEFKGVITYKVTIEGADVTD
                     EMKAYLPKSMTMTIKGNKSRTELIMSMMKTVSIADGDNKTAITLMDMMGQKFAIQSNY
                     EEIMKEMGEAPEAKVEVTGETKEIAGYVCKKAIITIAENDQELIVYFTDELGSSELNF
                     DNPMYKDINGVMLEFEMPNESFSMKFEATSVEKKNVDDSEFTIPEGYQVKTQEELKGM
                     FGG"
     gene            6390..8801
                     /locus_tag="PKM34_01985"
     CDS             6390..8801
                     /locus_tag="PKM34_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNQ82381.1"
                     /translation="MARNTNGDKAEQGTRPKKEKSRDPEKNKKRLPAAFTDGRFARIM
                     GFFFLLSGVFLLLAFTSYFMSWKLDSNVLDNNSAGYIFDSSVTVHNWTGKLGAWFAHI
                     FINRGIGIAAYLLLFLMILTGFRLTTGIALLPFGKAFRYSLLGILWISVTLGFALRHN
                     PDMQLMGGVFGYESSKWLTGLLGAPGTVLLLAFILISFVVIINLIPSLTKKKQPATAD
                     EALDGEQGVSGVPKEDVYNTREYAVSPEPEAVPTEFTLYHREKEAPPEKPAPTDKGIE
                     LTIEETPKEEPVSPKEPERFGLDTLYDPTKDLSDYKYPTLDLLSDYGSDSVPVAKEEL
                     EQNKDKIVETLNNYAIRIDKIKATIGPAVTLYEIIPAPGVRIAKIKNLEDDIALSLSA
                     LGIRIIAPIPGKGTIGIEVPNLKPEIVSIKSLLSSDKFKNSTFELPIALGKTISNESY
                     IADLTKMPHILMAGATGQGKSVGLNAILLSLLYKKHPSQLKFVMVDPKKVELTLFHKI
                     ERHFLAKLPDSEESIITDTRKVVRTLNSLCIEMDHRYELLKDAQVRNIKEYNAKFTSR
                     HLNPEEGHMFMPYIVMVVDEFADLIMTAGREIENPLTRLAQLARATGIHLVIATQRPS
                     VNIITGTIKANFPARFAYRVISKVDSRTILDSSGADQLVGKGDMLISTGSDLLRVQCA
                     FVDTPEVEKVTEFIGSQRGYPEAFALPEYYDENDEQEKGDFDPSARDELFEDAARIIV
                     QHQQGSTSLLQRKLKLGYNRAGRIIDQLEAAKIVGPFEGSKAREVLVANEMALEQFLK
                     DLYTK"
     gene            8817..9461
                     /locus_tag="PKM34_01990"
     CDS             8817..9461
                     /locus_tag="PKM34_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNQ82382.1"
                     /translation="MRILSFIMIAAFVIAGHATHAQAKKTSEEILKQVSEKTKSFSSI
                     KIGFTYNMDNASAKIHETETGILTVKGDKYRLDIAGQRVISDSKTSWTYVADANEVQI
                     NTVEDDDNALTPTKLLTSYSENYKSKLVNEINKDGRAVYVIDLKPNTEKNFTNVELQI
                     DKELYRIVRIAIQDKSGNTFSYIVNKFEPNVPVKDTDFTFDPKEFPGVEVIDMR"
BASE COUNT         2695 a         2306 c         2274 g         2206 t
ORIGIN      
        1 tttttatcta tctgggcgat gatcttgtta tcaaccaggc gggtattcgg attgaggtct
       61 tcttcggtag gataaaattt cttttttgct tcttctatcc ggttgaggga ataatcggca
      121 aaacggacca ggaaggataa ttgccagata taaagttcgt acaatttatc ggtactgcgg
      181 agtaactgtt tttctcctgc aaccagatcc tggccatccg actggaaaaa cgcgtacaaa
      241 gcctgtaatg ctttgaccct gaggtgcctt ctgctcagca tgattcgaaa tgaactgaat
      301 ttaaagctac aaaggtaaac ttttccggca ccgcgtcgga attttttatc tcctgccaca
      361 aaatataaat gcaacctttg aagttatttt gctgtcttag gagagtctcc agccaatcag
      421 ccgcaaaaaa gcatgaaagt gttgtgtgaa gaaatatttt tatttttgtg ataaatctga
      481 aggaataaaa acggaaaaga agatggaaga gtttggaaac aaagagatta gaaaagaaga
      541 ggttttttcc agggtgattc gtgccgggaa acggacctac ttttttgacg tgaaggctac
      601 ccgttcaggt gagaattacc ttacgatcac cgaaagcaaa agacggtttg acaatgagca
      661 gggaaagttt ttttacgaga aacacaagat ttttctttac aaagaagatt ttgagaagtt
      721 cctgacaggc ctcaaccagg taatgcattt tgtagagacc ggcgaaaaac cggaaggaat
      781 tgaagacgag gcgtttagtg gagaggaggc ccataccgac ttcgattttg atgatctggg
      841 agaccatagt aaagatacag atcattgatt ctatccggcg ttcgcatgtt tttcccgcgt
      901 gccgtcaaca tgcttatttt tagagcattt catcagttga taaattatca taattccagg
      961 caagccactg ccgtttttta ccttattttt gaccgttcaa gaaaaataaa ccatgggtaa
     1021 ggtcattgca ttagccaacc agaaaggggg cgtcggaaaa accacaactg cgatcaatct
     1081 cggcgccagc ctggctgttc ttgaatacaa aacgcttctg gttgatgccg atccgcaagc
     1141 caataccaca tcgggcgtgg gttttgatcc caaaaacatc aagaccagcg tttatgaatg
     1201 catcattgat gacctggatc ccaacaatat tcttctcaat acgcaaaccc cgaacctgga
     1261 cctgttgcct gcgcatatcg acctggtagg ggctgaaatc gagatgatca acctccccaa
     1321 ccgggagaaa atgatgcgtc gggtcgtgca gaaagtcagg cacaagtatg atttcatcct
     1381 gatcgactgc tcgccttcgc tgggcctggt tacgatcaac gccttaacag ccgccgattc
     1441 agtgatcatc ccggtacaat gcgaatattt cgccctcgaa ggcctgggaa aactgctcaa
     1501 taccattaag atcgttcaga cgaggctgaa tcctgaactc gacatcgaag gaatcctgct
     1561 gaccatgttc gatcagcggc tgcgcctttc caaacaggtg gccgaggagg tcaaaaccca
     1621 tttccagcaa atggtcttcg acactattat ccatcgtaac acacggctga gcgaagcgcc
     1681 cagtttcggt gaaacggtga tcatgcacga cgccaccagt atcggagcga tcaattatct
     1741 caacctggcc cgggaaatcc tccagaagaa cggaatgacc catctgagtg aagctgacaa
     1801 aacaatcgat atagttaatg acatctaaga aaaaagcgct tggccgggga ttggatgcca
     1861 ttctgcagag ccccgagacc gatatcacat cccgcgatat ctcgggcgat tacgtggcag
     1921 gagccgttgc cgaaatagag atcggaaaga tagaagccaa cccgtttcag ccccgtaccg
     1981 attttgaagc ggaaagcatg gaagaacttg cccgcagcat tggcgaacag ggtatcattc
     2041 agcctcttac ggtaagaaag gcgggattcg accgctacca gcttatcgcc ggcgaacgga
     2101 gactgaaagc ttcaaaaatg gcagggcttt ctacggtacc ttgttatatc cgcgtagcta
     2161 acgatgagca gatgctggaa ctggcgctta tcgagaacat ccaccggaaa gacctgaacg
     2221 ccattgagat cggcatcagt taccaacgtc tgatcgaaga actgaacctg acccaggaag
     2281 aggtcagtaa aaaggtcgga aaagaccgcg ccaccatttc aaactacatc cgtttgctga
     2341 aactcccggt tgaagtgcag tatgctctcc gcaacgacca gatttccatg ggccatgcgc
     2401 gcaccctgat cggcatttcc gatgaagaga agcaattgaa gacgctgctg aaaatcgtcg
     2461 aaaaaggatt atcggtaagg gaagcggaaa aactggcccg cgagatccag gcggaaccgg
     2521 ccgtaaagga accagctttg agcagccttc cggaacacct ccaggaagcg ggactgaaac
     2581 tccatgaaaa aatcggggcg gccgtaagaa tcaaaagtaa tacgaaaggg gccggttcgc
     2641 tcgttattaa attcggatcc ggtaatgaac tggagcgaat tttaaaactt ctgaacgaat
     2701 cgtgaacctc acttttcccg ataaaaatct aattctctcc tttctggctg tattattttt
     2761 gatattggcc tgtaaaacag cagcttccca ggatacaaca aagaccagga gagaaccggc
     2821 aaaacactct gttggcaaag cagccatttt ttcggccgtc ttgccggggt tgggtcaggc
     2881 ttacaaccgg aaatactgga aaatacctgt tgtgtatgcc ggttttgggg cgatagggta
     2941 ttttgtggtg accaacgaca agcaatacga cacattcaaa gaagcgtacc tgtatgtggc
     3001 caatgccgac acgacgccga tcgataatcc atatgtcaca aaatactcca aagaccagct
     3061 caggagctcg atggaatact accgccgtaa ccgcgacctg tcgatcatta tcggggcgtt
     3121 atggtacgca gtcgtcatcc tggaagctta cgccgatgca catttttttg attatgacgt
     3181 cagcgaagac ctgacgctga aagtaggccc ggcagcgctt ccatcaccgg aacggccacg
     3241 tcagccggta ccgggcatac atattacctt aaagttttaa acagtgaaaa taatcctttc
     3301 aggatacgga agaatgggcc atgaggtcga aaaagccgct atcgcccgcg ggcaccggat
     3361 tgttgcccgg atcgataccg aagacgattg ggcgacctgc ggcccgttgt ttagcagcgc
     3421 cgatgtagtg atcgatttca gccggcccgc atctgccgtt gccaacatcc ggcattgctt
     3481 cgatcatcat attcccgtgg taaccggaac gaccggctgg aataaagacg aaccccttgt
     3541 cagaaaatgg tgtgcggatg agaatcaatc ccttttcgtc ggaagcaatt tcagcatcgg
     3601 ggtaaatatc ctgttcgcac tgaccggcaa actcagcgaa ttgatgaacc ggtttgatga
     3661 atacaatatt tcactggaag agattcacca catccataag cttgatgcgc caagcggaac
     3721 ggcaatcaaa ctggccgaaa tgatcctgag ccgggtggag cggaaaaagt catgggtgaa
     3781 ccaaaccgaa cgtactcccg aagaattgca ggtgatatct gtcagggagg gggaagtacc
     3841 aggtacccat tctattgtct gcgattcgga ggccgaccgg atcacgcttc ggcatgaagc
     3901 aaaaaacagg aaaggatttg ccctcggagc agttatggcc gcagaatggc ttccgggcaa
     3961 gccaggatat tttgacatga acgaccttct cgggcttact ctataatttt caccggctta
     4021 acaaaaatta acagctaagt cccgcaacgg ttttccattt attcgttact ttcgcaacca
     4081 aatcacaact caatgaacat tttcctcctc cttttcatca tttttttcat cagttccctt
     4141 gttggtttat ggaaaatctt tattaaagcg ggatatcccg gatggacggt gctggttccg
     4201 ttttacaatt tctacatctg gcttaaaatc attaaaaaac cgctctggtg gtatattttt
     4261 ctcctcatcc catttattaa tgtattcacg atcctgctga tgatcgtgga actgttgaag
     4321 tgtttcaata aattcggact gggcgcccag gcactcggcg tacttttccc cttcgcctat
     4381 ctcccctatc tcgggtttac ggaaaaagaa aaatacattc atcccgacaa aaggaaagaa
     4441 ttcaagaaaa cctggcttcg cgaatgggtc gacgcgatca tttttgctgt catcgccgct
     4501 acgatcatcc ggacattcct gatcgaagcc tacacaatcc caacgccctc catggaaaaa
     4561 accctgctgg tcggggattt cctgtttgtg agtaaaatga gctacgggcc ccgcgtcccc
     4621 atgacccccc tctccttccc gttcgttcat cacaccctgc ccatgaccac ggagaccaaa
     4681 tcctacctgg aatggatcaa actgccctat taccggttcc cgggattcgg caaagtacaa
     4741 agaaacgatg cggtcgtctt taattatccg gatggcgata cactttccgt taagtttcag
     4801 agcaaccgga gttattattc catggtcagg gaatacggct ggtcgcgcgt gaaccggaat
     4861 tcaagggaat tcggagagat catttccagg ccggtcgata aaagggaaaa ttacatcaaa
     4921 cgctgtattg ccatagcagg cgatacgctt caaatcatcg accagcaggt ttatatcaac
     4981 ggcgagaaag ccttcaaccc gggtatcacc gaataccagt acctgattaa atcgaacatc
     5041 agccagctta atccacggtt gctcgacgaa ctggatgtaa ccgacaaggt ctggccggta
     5101 gggcccggag agttcgttgc tacgctgact gataaagctg caaacgaact gcgtcgagtc
     5161 aaagggatcg aatcggtgga ggtgctcatg gagccggccg gaaaatggga cagggaaact
     5221 ttcccctact ccggcgatta tccctggaac cgtgacaatt acggaccgat cgtgatgcca
     5281 aaagcgggta cttccatcga ccttacacta aaaaatctac ccttatacga acggatcatt
     5341 aagaattacg aactgaatga cctgaaagtg acggatggca ggatttatat caacggtgtg
     5401 gaatcctcac aatacacctt taagatgaat tattactgga tgatgggcga taaccgccac
     5461 aattcagccg acagccgcta ctgggggatg gtcccggaag accatatcgt cggaaaagcg
     5521 gtctttgtat ggttctctat ggataagaac aaatcgctct tcgatggcaa gatccgttgg
     5581 aacaagcttt tgcgcactgt caagtaatta agttttacct aaataaatta aaaaatgaaa
     5641 aaaagttcta tgttaacctg gctgctggca gccgtattta tgctgacttc ttttgcaact
     5701 tttgccaagg agtttaaagg cgtgatcacc tataaagtaa ctattgaagg ggctgatgta
     5761 accgatgaga tgaaagccta cctgcccaaa tcgatgacga tgaccatcaa aggcaacaaa
     5821 tcacgcaccg agctcattat gagtatgatg aagactgtat ccattgccga tggcgacaat
     5881 aaaacagcca ttaccctgat ggatatgatg ggccagaaat ttgccattca gtcgaattat
     5941 gaagagatca tgaaagagat gggtgaagca cccgaggcaa aagtggaggt caccggcgaa
     6001 accaaggaaa tagccggtta tgtctgcaaa aaagcgatta ttacgatcgc agagaatgac
     6061 caggaactga tcgtttattt tacggacgaa ctgggatcga gcgagctgaa cttcgataat
     6121 ccgatgtaca aggacatcaa cggcgtcatg ctggagttcg agatgcccaa tgaaagtttc
     6181 agcatgaagt tcgaagccac ctcggtggag aagaaaaatg ttgatgacag tgaattcacc
     6241 attccggaag gctaccaggt taaaacccag gaagagctga aaggaatgtt cggcggctga
     6301 gaagtaagca tccattactc ctgttcaaaa agtaaatcga caggcagggc aatttttctt
     6361 aattttgccg ttctgtaaaa aaaaccacaa tggccagaaa taccaacggg gacaaagctg
     6421 aacagggaac ccggccgaaa aaagaaaagt cccgcgatcc tgaaaagaat aaaaaacgtt
     6481 tgccggcggc atttaccgac ggccgttttg ccaggatcat gggatttttc tttttgctct
     6541 cgggcgtttt cctgcttctt gctttcacct cttatttcat gtcgtggaaa cttgacagca
     6601 atgttctcga caacaacagt gcggggtaca tctttgacag ttcagtaacg gtccataact
     6661 ggacaggcaa gctgggagcc tggtttgccc atatctttat caaccggggc atcggtattg
     6721 cagcgtattt gttgcttttc ctgatgatcc tcaccggttt ccggctgacg acaggcattg
     6781 cattactgcc ttttggcaaa gcgttccgtt acagtttgct gggcatcctc tggatttcag
     6841 taactctcgg gtttgccctc cgtcacaatc ccgacatgca actcatggga ggcgttttcg
     6901 gttatgaatc ctcaaaatgg ctgacaggcc tgctcggggc ccccggcacc gtgctgctcc
     6961 tggcctttat cctgatcagt tttgtcgtca ttatcaacct gataccttca ctcactaaaa
     7021 agaaacaacc cgcaacggct gatgaagccc tcgacggcga acagggggtc agtggtgttc
     7081 ctaaggaaga tgtttacaac acacgggaat atgcggtgag ccctgagccc gaagcggtac
     7141 cgaccgaatt tacgctgtat caccgggaaa aagaagctcc tccggaaaaa ccggcaccga
     7201 cagataaagg aattgaactg acgattgagg agacgccaaa agaagagccg gtcagcccga
     7261 aagaaccgga aagattcggc ctcgatacac tgtatgaccc gacaaaagat ctttccgatt
     7321 acaagtaccc aaccctcgat ctattgtcgg actatggctc cgactcagtt ccggtagcca
     7381 aagaggagct ggagcagaac aaggataaga tcgttgagac tttgaataat tacgccatcc
     7441 ggattgataa gatcaaagcg acgatcggtc cggcggtgac gctttacgaa atcatccctg
     7501 cacccggagt gcggattgcc aagatcaaga acctggaaga cgacattgcc ttatcgctct
     7561 ccgcgctggg aatccgaatc atcgcaccga tccccggtaa agggacgatc ggtatcgagg
     7621 tgccgaacct caaaccggag atcgtatcga tcaaatcgct gttatcttcc gacaaattca
     7681 agaattcgac attcgaactc cccattgcac ttggcaaaac gatctccaac gaaagctaca
     7741 tcgccgatct gaccaaaatg ccgcacattt tgatggccgg cgctacgggc cagggcaaat
     7801 cggtcggcct gaacgctatc ctgctttcgt tgctgtataa gaaacatccc agccagctca
     7861 agttcgtcat ggtcgacccg aaaaaagtcg agctgaccct tttccacaag atcgaacggc
     7921 atttcctggc caagctgccc gattcggaag aatcgatcat caccgatacg cgcaaggttg
     7981 tccggaccct gaattcgttg tgcatcgaga tggatcaccg gtatgagctg ctgaaagacg
     8041 cccaggtgcg caatatcaaa gaatataacg ccaaattcac atcccggcac ctgaatcccg
     8101 aggagggcca tatgttcatg ccttacatcg tgatggtggt ggatgaattt gccgacctga
     8161 tcatgactgc ggggagggag atcgaaaatc ctttgacccg gctggcacaa ttagcccgtg
     8221 caaccggtat ccatcttgtc atcgccactc agcggccatc ggtcaacatc attaccggta
     8281 cgatcaaagc caacttcccg gcccgttttg cctaccgcgt catttcgaaa gtcgactccc
     8341 gtaccatcct cgacagttcc ggggccgacc agctcgtcgg aaaaggcgat atgctgatct
     8401 ccaccggcag cgacctgttg cgcgtgcaat gcgcattcgt cgacacgccg gaggtagaga
     8461 aagtgaccga attcatcggc tcacagcgcg gctacccgga ggcctttgcc ttaccggagt
     8521 attacgatga gaatgacgaa caggagaaag gcgatttcga tcccagcgcc cgggatgaac
     8581 tgttcgagga tgccgcccgg atcatcgtcc agcaccagca aggctccacg tcgctgctgc
     8641 aacggaaatt gaaactgggg tacaaccggg ccggccgcat catcgatcag ctcgaagcgg
     8701 ctaaaatcgt cggcccgttc gaaggctcca aagccaggga agttcttgtc gccaacgaaa
     8761 tggctttgga acagtttttg aaagaccttt acactaaata attaacaaac ctgaccatgc
     8821 gaattttatc attcatcatg attgctgcct ttgttatcgc agggcatgct actcatgcac
     8881 aagcgaaaaa gacatccgaa gagatattaa aacaagtatc cgagaaaaca aaatcatttt
     8941 catccataaa aataggattt acgtacaata tggacaacgc ttcggccaag atacacgaaa
     9001 ccgaaaccgg catactgact gtaaaaggtg ataaatacag gcttgatatt gcgggccagc
     9061 gcgttatatc cgacagcaaa acatcatgga cctatgtcgc cgatgccaat gaagttcaga
     9121 tcaacacggt ggaagatgat gacaatgccc ttactcccac aaagcttctg acctcataca
     9181 gtgagaatta caaatcaaag ctggtcaatg agattaacaa agacggcagg gctgtttatg
     9241 ttatcgatct aaaacccaat accgagaaga atttcaccaa tgtggaactg cagatcgaca
     9301 aggaacttta ccggattgtg aggattgcca tacaggacaa gagcgggaat acgttcagtt
     9361 atattgtcaa caaattcgag ccgaacgttc cggtaaaaga taccgatttc actttcgatc
     9421 cgaaagagtt tcccggtgtg gaagtgatcg atatgaggta aaatcagtag cccagcttcc
     9481 a
//
LOCUS       DAOQXS010000036         9372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_7828_length_9372_cov_3.334550, whole genome shotgun sequence.
ACCESSION   DAOQXS010000036 DAOQXS010000000
VERSION     DAOQXS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7828_length_9372_cov_3.334550"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="PKM34_01995"
     CDS             complement(<1..1698)
                     /locus_tag="PKM34_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ82383.1"
                     /translation="MQRRLFIFKLLIIILFNGLFLRNVAQENALITLSPEERWADSAL
                     NTFSTDEMIGQLMIVRANVPNQPYFDVIERYIKDYNLGGVTFFGGHPALQALQTNRWQ
                     SMAKTPMFISIDGEWGPAMRLDSILSFPYLMTAGAAVDDSLMYFMGMEVARQCKRLGI
                     QINFAPVVDINSNPDNPVIHMRSLGEDREKVAAKGIMYMKGMQDGGLIVTAKHFPGHG
                     DTGTDSHYTLPVVPHSRQRLDSLELYPFRKLIEAGLDGIMIAHLFVPAVENSPNTPST
                     LSKAIVTDLLRGDLGFGGLIVTDALDMKGVTLNDQPGEIEVKALKAGNDILLLSANVP
                     EAVKKIKEAIEHGDLPLELIRERCRKVLLYKYKAGLNRYNPVELTGLYDDLNKPSSEL
                     VCRNIFEQSVTLVHDDHNMLPVIGLDSLKIASVSTGYGALTPFQERLGCYAPVDHFFL
                     KKEPTDEEIIALKKKIAPYNLVILSVQNTSIWGGSPYGISPKIIQLIGDISRSHRVIL
                     DLFASPYALRLIEKLPEPAAILLSYQDHPLIQDISAQAIFGGTAISGRLPVTAGNSYP
                     "
     gene            1875..3236
                     /locus_tag="PKM34_02000"
     CDS             1875..3236
                     /locus_tag="PKM34_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ82384.1"
                     /translation="MIGTVAAFAQKTQKTDPVQDSKPVTRILFVFDASQSMYGRWQSD
                     LKINIARDILLKVLDSLRPMPNLEVALRLYGHQHRFPPQVCDDSKLEVPFAKDNYDKI
                     RLKLKDVIPRGTSPIAASLEQAAGDFPPCENCRNVIVLITDGIEECGGDPCQASYTLQ
                     KSGIALRPFIIGIGANFEKAFDCVGIYLDASSEEKFSNALHIVVSRIMNPTTCQVNLL
                     DANNRPTETNIGMTFYDHSSGKIKYNIVHTLNSYGQPDTLNIDPMVTYDIVVHTIPPV
                     RADSVRLVPGRHTVIPVNVPQGILLLKMNSQNTNLKDITTIVRKEGRSETINTQEIDQ
                     QEKYITGTYNIEVLSLPRLYRNNVDIIQSHTTTVEIPVPGIAVIQKPVKGFGSLFLEK
                     DGELELIYNFKDNDMGQESLVLMPGNYRAVFRSRYAYRSAFTIERAFEVKSGTTTNVK
                     LQD"
     gene            3257..3784
                     /locus_tag="PKM34_02005"
     CDS             3257..3784
                     /locus_tag="PKM34_02005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNQ82385.1"
                     /translation="MLIFLIGYMASGKSTVGRMLAEELKYRFIDLDSEFEKRTGASVP
                     NYYTQFGELAFREKEREVLRLHLNDTDSVIATGGGTPSYHDNMVLMNRSGRTIFLDVP
                     VNILVRRLAGEQSRRPVIKEIPDEELASFITPHLDSRRKYYDLASMKVQVINENAEEI
                     VAQLIQRLSRTAPTL"
     gene            3839..4414
                     /locus_tag="PKM34_02010"
     CDS             3839..4414
                     /locus_tag="PKM34_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ82386.1"
                     /translation="MSDLDALKNLVLAPYILKATALIGVSRKVGGNQFRHSFSTLGIL
                     MDYKYFNDSILLKASLLHDLLEDLPETQVDEIRWIDEDASQVVDLVLEVTRRPDESKP
                     QYLQRVLETGSVRAKILKCADRISNLTDLHRDTHPDHKISDYLEQTETYILPMARQVS
                     ADMVRELTDLIAKRRKIIQRMNAGSGDGMMV"
     gene            complement(4411..5139)
                     /locus_tag="PKM34_02015"
     CDS             complement(4411..5139)
                     /locus_tag="PKM34_02015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNQ82387.1"
                     /translation="MVIYIATFVITIIFWLWIIRKFDRFEREPLKNILFVFIAGGLLS
                     IIPAGLFNGLFAWMIGYGFEPGSDAGIGFGRLLWFYGFVGFNEEFWKALATIILIRRM
                     KSFNEPADALVYSMTVAFGFSVIENIEYTLKLGYPVFWVRQFNAVPLHVGLAAIWGIG
                     IAKVRFSNAPSYLSRVMPYILIAAFLHFIYNIAATRIFNTALSLLIPTVIAFFLILFA
                     IRRVKKYSEEGPFRDGLGDGVSDA"
     gene            complement(5250..6689)
                     /gene="rlmD"
                     /locus_tag="PKM34_02020"
     CDS             complement(5250..6689)
                     /gene="rlmD"
                     /locus_tag="PKM34_02020"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNQ82388.1"
                     /translation="MSRRSKNRQPLPLLEKVEIIDAGAEGKAVARVENRVVFIPFGVP
                     GDIVDVQVTGRKKSFFEGRIVKFHVMSSSRVEPACVHFGLCGGCRWQNMDYDRQLHFK
                     QKQVQDNFDRIGHFKYPEIRPILGSDNIYFYRNKLEYTFSNRKWFTGARPEAGSNENT
                     NGLGFHLPGMFDRILDIEKCHLQADPSNEIRQAAREYAFENNLSFYDVKTWQGLMRNL
                     VIRTTQAGEVMVIVVFHDEEDEVIRGFLSFLERRFLEITSLLYVINGKHNDDISDQEI
                     RLFAGKPWLEEILKAFHPGDPPLKFRIGPVSFFQVNVKQAEKLYSTAVEMAGFKGQET
                     VYDLYSGTGTIACYIAKYVKKVVGLEYIPSAVADAFENAGLNGIDNVDFMAGDIAKIL
                     DDDFFEKHGRPEVIITDPPRSGMHEKVIRQILAARPEKVVYISCNPATQARDIQLLSE
                     HYEVLAVQPVDMFPHTQHVENIALLVKRE"
     gene            6815..8326
                     /locus_tag="PKM34_02025"
     CDS             6815..8326
                     /locus_tag="PKM34_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNQ82389.1"
                     /translation="MKILPVEKIREADAYTIAHEPVESVDLMERAAMKCYNWLKKRVD
                     ADRPVKIFCGPGNNGGDGLAIARMMAESHHQVSVYVIRYSEKSSRDFLINYERLTGLS
                     SVPVFDIKDGDRLPEISEEDVVVDALFGSGLNKPAEGFSAAVIRHINASRSLILAIDM
                     PSGLFCDMPTDEKAGPVVRADYTLTFQAPKLAFLFAENDIRVGEWVVLDIGLHPGFTD
                     SLEVKNFLLTREMASGLLKPRTKFAHKGHFGHALLIAGGYGRMGAAVLAAEACLRSGV
                     GLLHAHAPAKGVNVIQTAVPEAMITIDADEECLSSLPDLSPFTAIGIGPGIGFDMKTK
                     NALKLLIQNTSVPMLFDADALTILAENRTWISFVPRNSVFTPHPKEFERLAGKATDDF
                     HRNRLQREFCIRHGVWIVLKGAHSCICGPDGVCYFNTTGNPGMATGGSGDVLTGLILG
                     LLAQRYSPRDACLLGVFLHGLAGDLAAKKIGMASLLAGDITRNIGKAFKKLSS"
     gene            8357..>9372
                     /locus_tag="PKM34_02030"
     CDS             8357..>9372
                     /locus_tag="PKM34_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82390.1"
                     /translation="MIRITDKAKLREIVTGTLLGLLVAIVTFPAPDWSYGPGIDPPLA
                     WVFNSLFLNGSGSDPVIIFPHGPLAFFTYPTAENAWLVMIVTIFLKIMLILSIKQFIT
                     AGKTWTKWLAASAVTYLVSVSGGFSHLLLANIMAGYAAGFHQGFSKWTARYFLVLLLT
                     AIAFYVKAYTAIIAGILFISSGIYHLIKTKKVLPFLTDSFILLIIILLFWLFLFRSFH
                     GFITYVNGMVHLAQDNSSAAAYYPYNNWLVLSSFILLIIIIIIVNRNKESLFFSSLMG
                     LSLFAAWKHGMAREDIYHVMGFFVFVMMSLALFILFSGKRLIINILIATAALFLLSLN
                     SKNA"
BASE COUNT         2321 a         2403 c         2337 g         2311 t
ORIGIN      
        1 gggatagctg tttcctgccg taaccggtaa cctgccgctg atggctgttc cgccgaatat
       61 tgcctgtgct gaaatatcct gtatcagcgg atgatcctgg taggagagga gaatggccgc
      121 cggctccggg agtttttcga tcagccttag cgcgtaaggc gaggcaaaaa gatccaggat
      181 gacccgatgt gaacggctta tgtcgccgat gagttgtatg atcttcggtg aaatgccgta
      241 tgggcttccg ccccagatgg aggtgttctg aaccgaaaga ataacaaggt tatatggcgc
      301 tatttttttc ttcagcgcga taatttcctc atcggttggt tcctttttca ggaaaaaatg
      361 atcgaccgga gcataacatc caagcctctc ctggaagggg gttaatgcac cataaccggt
      421 tgaaacagaa gcgatcttca acgaatccaa cccgatgacc ggaagcatgt tgtgatcgtc
      481 atgcaccagc gtcacagact gttcgaagat attccggcaa acgagctcag acgacggttt
      541 gttgaggtcg tcatacaatc cggtcaattc aaccggatta taccggttca gcccggcttt
      601 atatttatac agcagcactt tccggcaccg ttcccggatg agttcgagcg gcagatcacc
      661 gtgttcaatg gcctctttga tcttcttcac cgcttccggg acattggcgc tgagaagtag
      721 aatgtcgttt cccgctttga gcgcctttac ttcgatctct ccgggctggt cattcagcgt
      781 cacccctttc atgtcgaggg catcggttac tatcaaaccg ccaaatccga ggtcaccccg
      841 cagcaggtcg gttacgattg ctttcgaaag agtcgaaggg gtgttcggcg aattttcaac
      901 ggccgggaca aacaggtggg cgatcatgat cccgtcaagt cctgcttcga ttagctttct
      961 gaaaggatac agttccagtg aatcgagccg ctggcgcgaa tggggcacga caggtagcgt
     1021 ataatgggag tcggtgcccg tatcgccatg gccgggaaaa tgtttggccg tcacgatcaa
     1081 acctccgtcc tgcatccctt tcatatacat gatgcccttt gcggctactt tttcccggtc
     1141 ttcacccagc gagcgcatgt gaataacagg gttgtcggga ttggaattga tgtcgacgac
     1201 cggggcaaag tttatttgga ttccgaggcg tttacattgc cgcgctacct ccattcccat
     1261 gaagtacatc aggctgtcgt caacggcagc gccggcagtc atcaggtaag gaaaagacaa
     1321 aatgctgtcg agacgcatag ccggccccca ctcaccatca atggaaatga acataggagt
     1381 ttttgccata ctttgccagc ggttggtttg cagggcctgc agagccggat ggccgccgaa
     1441 gaaagtcact ccaccaagat tgtaatcttt tatatatctt tcaattacat caaagtaagg
     1501 ctgattcggc acattagccc tgacgatcat aagttgcccg atcatttcgt cggtggagaa
     1561 ggtattcagg gccgagtcgg cccagcgttc ttcaggtgat aaagtgatta aagcgttttc
     1621 ctgtgcaaca tttcgaagga ataatccgtt aaacaatata attattaaga gtttgaagat
     1681 aaaaagtctg cgctgcatga tttccttttg tttcaaaatg gataaacata atctccggtc
     1741 aaaaatacaa cacaagaacc gggaggatat gatcgctgtt tcattttttc aacaatttga
     1801 tcttaaacat tgataaaccc acgggaagag aagtgataag gatgattcgg attctgctaa
     1861 tgacctggct gataatgatc ggcacggttg ctgcttttgc ccagaaaacg caaaaaacgg
     1921 atcccgtgca ggattcaaaa ccggtcaccc gcatcctttt tgtgttcgat gcttcgcaga
     1981 gcatgtacgg ccggtggcaa agcgacctga agatcaacat tgcccgtgat atcctcctga
     2041 aagtacttga cagcctccgc ccgatgccca acctggaggt ggctttgagg ctatacggcc
     2101 accagcatcg atttccgccc caggtttgcg atgactccaa actggaagtc ccgtttgcaa
     2161 aggataatta cgacaagata aggctcaagc tgaaggatgt cattccccgg ggaaccagcc
     2221 cgattgctgc gtcgctggag caggctgccg gcgatttccc cccctgcgaa aactgccgga
     2281 atgtaatcgt actgattacc gacggaatcg aggaatgcgg tggcgacccc tgccaggcat
     2341 catacacgct ccagaaatcg ggtatcgccc tccggccatt tattattggt atcggggcca
     2401 actttgaaaa agcgttcgac tgtgtcggca tctacctcga tgcctccagc gaggaaaaat
     2461 tcagcaacgc gctgcatatc gtggtctcca ggatcatgaa ccctacgacc tgccaggtaa
     2521 atctcctgga tgccaataac cggccgaccg aaacaaatat cggaatgacc ttttacgatc
     2581 attcgtctgg gaaaataaaa tacaatatcg tccatacgct taacagctat ggtcagcccg
     2641 atacgttgaa catcgatcca atggtcacgt atgatattgt ggtgcatacc attccgccgg
     2701 tgagagccga cagtgtcagg cttgtgccgg gccggcatac ggttattccg gtgaatgtgc
     2761 cgcagggaat tttattgtta aagatgaatt cccagaatac aaatttgaaa gatatcacaa
     2821 cgatcgtcag aaaggaagga aggagcgaga ccatcaatac gcaggagatc gaccagcagg
     2881 aaaaatatat taccgggacg tataatattg aggttctgag ccttccgagg ttatacagaa
     2941 ataatgtaga catcatccaa agccatacca ctacagtaga aatcccggtt ccgggcatag
     3001 ccgtaattca gaaaccggtg aaaggattcg ggagcttgtt ccttgaaaaa gatggtgagc
     3061 ttgagctgat ctacaatttt aaagataacg acatggggca ggagtcgctg gtgctgatgc
     3121 ccgggaatta cagggccgtt ttcaggtcgc ggtatgccta ccggtcggct ttcaccatcg
     3181 aacgggcttt tgaagtcaaa tcggggacaa cgaccaatgt taagctccag gattaacctc
     3241 atctgaagcc atttctatgc taatatttct tatcgggtat atggcatcgg gcaaatcaac
     3301 cgtcggccgg atgctggctg aggagttgaa gtaccggttc atcgatctgg attcagaatt
     3361 tgaaaaacgc acaggagcct ctgttccaaa ttattacacg caatttggtg agttggcctt
     3421 ccgggaaaag gagcgggagg ttttgagact ccatctcaac gataccgatt cggtgattgc
     3481 taccggtgga ggaactccgt cgtaccacga caacatggtg ctgatgaacc gttccggcag
     3541 aactatattt ctcgatgttc cggtgaatat cctggttaga cgcctggccg gcgagcagtc
     3601 acggcgcccg gtaattaaag aaattcccga tgaggaactg gcatctttta tcactcccca
     3661 cctggattca aggcggaaat actatgatct ggcttcgatg aaagttcagg ttattaacga
     3721 gaatgctgaa gagattgtcg ctcaattgat tcaaagactt tccagaactg ctcccactct
     3781 gtgactgatc agctccattt ttccattaat tttgtcattc taaacaaaaa ctaataagat
     3841 gagcgattta gacgccctga aaaacctggt actggcgcct tacatcctga aagccaccgc
     3901 cctgatcggt gtgagcagga aggtgggagg aaaccagttc cggcacagtt tttccaccct
     3961 gggcatactt atggactaca aatacttcaa tgacagcatt ttactgaaag cctcgctgtt
     4021 gcatgacctg ctggaggatc tcccggagac gcaggtcgat gagatccgct ggattgatga
     4081 ggacgccagc caggtcgtcg acctggtgct ggaggtgacc cgccggcccg acgagtccaa
     4141 accgcaatac ctgcagcggg tgctggaaac cggctccgtg cgggccaaga ttctcaagtg
     4201 cgccgaccgg atcagcaacc tgaccgacct tcaccgcgat acccatcccg atcataagat
     4261 cagcgattac ctggaacaga ccgaaaccta tattttgccg atggcccggc aggtcagcgc
     4321 cgatatggtc agagaactga ccgacctgat tgcgaaacgc aggaagatca tccagcggat
     4381 gaatgccggc agtggcgacg ggatgatggt ttaggcatct gagactccat ctcccagtcc
     4441 atcccggaac ggcccttctt ccgaatattt ttttaccctt ctgatggcga acaggatcag
     4501 gaagaatgcg ataactgtag gtatcagcag gctgagcgcc gtgttgaaaa tgcgcgtggc
     4561 ggcaatgtta taaataaaat gcaggaaggc ggcgatcagg atatacggca tcacgcgcga
     4621 caggtaactc ggggcgttgc tgaatcttac tttcgcaatt ccaattcccc agatggcggc
     4681 cagccccaca tgcaacggca ccgcattgaa ttgtcgtacc cagaatacgg gatagcccag
     4741 ttttaaagta tattcgatat tctcgatgac ggaaaatcca aaagccaccg tcatcgaata
     4801 aaccagcgca tcggcaggtt cattgaagct cttcatccgg cggatcagga tgatcgtcgc
     4861 cagcgctttc cagaactctt cgttgaaccc gacaaaacca taaaaccaaa gcaacctgcc
     4921 gaaccctatg ccggcatctg atcccggttc gaatccgtaa ccgatcatcc aggcgaataa
     4981 accgttgaat aatcccgccg ggataatact caataatccg ccggcaatga aaacgaaaag
     5041 aatgttcttc agcggctccc gctcgaaacg gtcgaacttc cggatgatcc agagccagaa
     5101 gataatcgtg atcacaaatg tagcaatgta tattaccata atcgtaagta agcagtcagc
     5161 agtcagcagt cagcagttag atgttgttag ttgcttgttc cgatgacgta gccctaaggt
     5221 tctgttatta attcattatc aatctgttac tattcccttt tcactaacaa agcaatgttc
     5281 tccacatgct gcgtatgcgg gaacatgtcg acgggctgta ccgccagaac ttcgtagtgt
     5341 tccgacaaga gctggatgtc gcgggcctgt gtggccggat tgcaactgat gtacaccact
     5401 ttttccggcc tggcggccag aatctgccgg atcacctttt catgcatccc gctgcgcggc
     5461 ggatcggtga taatcacctc aggcctacca tgcttttcga aaaagtcatc gtccaggatt
     5521 ttagcaatat cgcccgccat aaaatcaaca ttatcgatcc cgttcaaccc ggcgttctca
     5581 aatgcatcgg ccacagccga tgggatatat tccagcccga ctaccttttt tacatatttg
     5641 gcaatatagc aggctatcgt gccggttccc gaatacaggt cgtataccgt ttcttgacct
     5701 ttgaatccgg ccatttctac agcggtcgaa tagagctttt cggcttgctt tacgttgacc
     5761 tgaaagaagg ataccggccc gatgcggaat ttcagcggag gatcgccggg gtgaaaggct
     5821 ttcaggatct cttccagcca gggctttcct gcaaacaacc tgatctcctg gtcggagata
     5881 tcatcgttgt gcttgccgtt tataacatac agtagtgatg tgatttccag aaaccgcctc
     5941 tcaagaaacg atagaaaacc ccgtatgacc tcatcctcct catcatgaaa aaccacaatg
     6001 accatcacct cgccggcctg cgtcgtgcgg ataaccaggt tacgcatcaa gccctgccag
     6061 gttttaacat cgtaaaaact gagattgttc tcaaacgcat actccctggc tgcctggcgg
     6121 atctcattgg atggatcggc ctgcaaatga catttttcaa tatccaggat ccggtcgaac
     6181 atgccgggca ggtgaaaacc cagtccgttg gtgttttcat tgcttccagc ttccggccgt
     6241 gccccggtga accatttgcg gtttgagaat gtgtattcga gcttgttccg gtagaaataa
     6301 atattatcgc tgccaaggat cggcctgatt tccgggtatt taaaatgtcc gatccggtcg
     6361 aaattgtctt gcacctgttt ttgcttgaag tgaagctgcc ggtcgtaatc catgttctgc
     6421 caccggcatc cgccacacag gccgaaatgg acgcaagccg gctcaacccg cgatgaagac
     6481 atcacatgaa atttgacgat tctcccttca aagaacgatt tcttcctccc ggtaacctgc
     6541 acatccacaa tatcaccggg gactccgaac gggatgaata ccacgcggtt ttcgacacgt
     6601 gccacggctt tgccttcagc gcctgcgtcg atgatctcca ccttctccag taaaggcaac
     6661 ggttgcctgt ttttcgacct tctgctcatt tcacggtaaa aataatgttt attgccgatc
     6721 ggtttattat aaaagaggaa attcaatttc cgcgtagcgg atcaattatt gtaacaggtc
     6781 aaacactacc tttgtatgaa ataccagata tgccatgaaa atcctgcccg tcgaaaagat
     6841 acgcgaagcg gatgcctata ccatagctca tgagcctgtc gaatcggtcg atctgatgga
     6901 acgggccgcc atgaaatgct acaactggct gaaaaagaga gtggatgcag accggccggt
     6961 gaagatattc tgcggaccgg ggaacaacgg cggcgacggg ctggcgatcg cccggatgat
     7021 ggccgaaagc catcaccagg tttcggttta tgtgatccgc tattcggaaa agtcgtcccg
     7081 ggattttctg atcaactacg aacggctgac cggcctcagc agtgttcctg tttttgatat
     7141 aaaagatgga gaccgtcttc cggaaatcag tgaagaggat gtagtggtcg atgctctttt
     7201 cggttcgggg ctgaataaac cggcggaggg tttttccgcc gcagtcatac ggcatatcaa
     7261 tgccagccgg tcgctgatcc ttgccatcga catgccgtcg ggcctgttct gcgacatgcc
     7321 cacggatgag aaagccgggc cggtcgtcag ggccgactat acccttactt tccaggcgcc
     7381 caagcttgcc ttcctgttcg ccgaaaacga tatcagggta ggcgagtggg tggtgctcga
     7441 catcggcctt cacccgggtt ttaccgacag cctggaggtt aaaaatttcc tgctgactcg
     7501 cgaaatggcc tcgggactgc taaaaccccg caccaaattt gcccacaaag ggcattttgg
     7561 ccatgcgttg ctcattgccg gcggctacgg ccggatgggg gccgccgttc tggccgcaga
     7621 ggcctgcctc cgctcgggcg tcggcttgct gcatgcccat gccccggcaa agggggtgaa
     7681 cgtgatccag acagcagttc ccgaagcgat gatcacgatc gacgctgacg aggagtgcct
     7741 ctcatcattg cccgatctgt cgcccttcac tgccattggc atcggcccgg gcatcggttt
     7801 tgatatgaag accaaaaatg cacttaaact cctgatacag aacacgtccg ttccaatgct
     7861 ttttgatgcc gacgcgctga cgatcctggc cgagaaccgg acatggatct cgttcgtgcc
     7921 ccgaaacagc gtcttcacac cccatccgaa agaatttgaa aggctggcgg gaaaagccac
     7981 cgatgatttc caccggaacc ggctgcagcg cgaattctgt atccggcacg gcgtctggat
     8041 cgtgctgaaa ggagcccatt cctgcatctg cggtccggac ggagtttgct atttcaacac
     8101 taccggcaac ccggggatgg caaccggcgg cagcggcgac gtgctcaccg ggctgatcct
     8161 gggactgctg gcgcagcgct atagtccgcg cgatgcctgc ctgctgggcg ttttcctcca
     8221 tggcctggcc ggagacctgg ctgcgaagaa aatcgggatg gcgtcgctgc tggccggcga
     8281 tatcaccagg aatatcggga aagcgtttaa aaagttatct tcctgatatt aaatatctta
     8341 attaccacca ttctgaatga tccggataac cgacaaagcg aagctgagag aaatagtcac
     8401 cgggaccctg ctgggattgc tggtagccat cgtcactttt ccggcgcccg actggtcata
     8461 cggcccgggc attgaccctc cgctggcctg ggtgtttaac tccctgttct tgaacgggag
     8521 tgggagcgat ccggtaatca tcttccccca cggacctttg gctttcttta cctatccgac
     8581 ggctgaaaat gcctggctgg tgatgatcgt gacgatattt ctcaagataa tgctgattct
     8641 aagcatcaag caatttatta ctgccggaaa gacctggacg aagtggctgg ctgcatcggc
     8701 tgtcacttac ctggtctccg tgagcggcgg attctcccac ctgctcctgg cgaatatcat
     8761 ggccggttac gccgccggat ttcatcaagg gttttcgaaa tggaccgcga gatacttcct
     8821 ggtgctgctg cttacagcga tagctttcta cgttaaagcc tacacggcta tcattgccgg
     8881 gatcctgttt atttcttccg gaatttatca tctcattaaa acaaaaaagg tattaccttt
     8941 tttgaccgat agctttatat tgctcattat catactttta ttctggttat ttctgtttcg
     9001 gagcttccat ggattcatca cctatgtgaa cggcatggtc cacctggcac aggacaactc
     9061 atccgcggca gcgtattatc catacaacaa ctggcttgtt ttgtcctcat tcatattatt
     9121 gattatcatt attataatcg taaaccggaa taaagaatcg ctcttcttta gctccctgat
     9181 gggattaagc ctcttcgctg cctggaaaca tggaatggcc agggaagata tttatcatgt
     9241 gatgggattc ttcgtatttg taatgatgag cctggccctg ttcatccttt tttcaggtaa
     9301 acgacttatt attaatattt taattgcaac agccgccctg tttcttttgt cgctcaatag
     9361 taaaaatgcc at
//
LOCUS       DAOQXS010000037         9135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8095_length_9135_cov_3.639537, whole genome shotgun sequence.
ACCESSION   DAOQXS010000037 DAOQXS010000000
VERSION     DAOQXS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8095_length_9135_cov_3.639537"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKM34_02035"
     CDS             <1..214
                     /locus_tag="PKM34_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNQ82391.1"
                     /translation="LLIIHGTGDDNVHYQNAEMLLNELVKHNKKFQFMPYPNRTHGIY
                     EGEGTLLHLSTLYTDFLKSNCPPGGR"
     gene            complement(260..1312)
                     /locus_tag="PKM34_02040"
     CDS             complement(260..1312)
                     /locus_tag="PKM34_02040"
                     /inference="COORDINATES: protein motif:HMM:NF008607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNQ82392.1"
                     /translation="MGEGIINTAIVGFGLSGRVFHAPFIHTHPGFRLVTVVERHSSLS
                     KEIYPGVTIAKEYNNVLNDGSIELVVLATPNIYHFEQARSALEAGKHVVVEKPFTPTS
                     QEADALIGIAEKTGKKIFVCQNRRWDGDFKTIQQVVYNGYLGEILEYEAHFDRYAPGA
                     RRSAWRDEPLPAGGVLYDLGSHLVDQALVLFGMPRSVYADIRMQRAESKVDDYFHVHL
                     YYERLKVILKASVFVKEQGPRYSLHGTKGSFIKYGIDPQEEMLKAGAMPGSEDWGKED
                     PDYWGLLNAEMHGQQFYGNIETEPGNYMAFYDNVFDVITKGAEQAVTPQEARNVIRIL
                     ELAFESQRAQSLVKIA"
     gene            complement(1319..2167)
                     /locus_tag="PKM34_02045"
     CDS             complement(1319..2167)
                     /locus_tag="PKM34_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNQ82393.1"
                     /translation="MITRRDFLKTTTLATASALLAPGILRAAPHKKDIGLQLYTVRDH
                     ISKDLPGTLQAVAKIGYTRLEAAGYNDGKFYGMAPGEFRSLVSGSGMQPVSSHVAFSP
                     EQSRHVIDAHLELDVPYVVYPWISMPEIPSADDYLRAAGLFNRLGEACKKDGLRFGYH
                     NHDFEFKKIGDTTGFDILLKNTDPDLVCFEADIYWMQYANIVPGDYLSKYPGRFELWH
                     IKDMDSGTERTFTEVGEGTIPYGDYFTNFKDISGMKYFFVEQDKCNRDSLESTKISYR
                     NLKKIL"
     gene            complement(2264..3793)
                     /locus_tag="PKM34_02050"
     CDS             complement(2264..3793)
                     /locus_tag="PKM34_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ82394.1"
                     /translation="MERRDLLKSMVSVPVLGALAYAFYRKRKFEKYLRDNIQEELKID
                     TEPAALTGDPSKGREIRLGLIGYGIRGKDLALAAGFAHPTMIDDWKEGAVNNRQDKRY
                     EEYQQQEPLNVRVTAVCDIFDTYGNMAVEAGSNVHREGTGGKMGQQPKRFLNHLDLIN
                     SPEVDAVIIATPDHWHGPMTIAAARAGKHVYCEKPLTWTVEETYEVRKAVKENNIVFQ
                     LGHQGRQNESYLKAKEVISQGILGKVNLIEVTTNRNDPNGAWVYPIHETAGRQTIDWE
                     QFIGQAPWHDFSLERFFRWRCWWDYSTGLSGDLLTHEYDAMNQILGLGIPDSAISSGG
                     IYFFKDGRTVPDVLHTVFEYPGRDLTLMYSATLASNRRRGRVIMGHDGTMELGSSMTV
                     YADRGSTRFREKLDNGVIDPDLPILTYTPGLKNVDAITSPTEQYFAGRGLMYTYRGGK
                     RVDTTYLHIREWLDCIRNGGQPSCNIDQAFEEAMTAHMGTMAYKENRRVFWDPVGEKI
                     L"
     gene            complement(3937..4401)
                     /locus_tag="PKM34_02055"
     CDS             complement(3937..4401)
                     /locus_tag="PKM34_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNQ82395.1"
                     /translation="MTNTQKLTLVVLRLAIGWHFLYEGLVKLANPNWTSAGYLLDSGG
                     FLKGFFISLASNPDMLKIVDLLNIWGLILIGLGLILGLFTRTAIISGIVLMAFYYLSH
                     PPFVGLKYAVPSDGSYLVINKMVIEGLALAVLLLFPTWKEWGVDRWVGRRRD"
     gene            complement(4467..5072)
                     /locus_tag="PKM34_02060"
     CDS             complement(4467..5072)
                     /locus_tag="PKM34_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82396.1"
                     /translation="MVHLLIGLLLLNCSGPCNSDLQSDPVINSVFSQSEIKDLSIILD
                     FFTDQICSKHLMDSANTQKCFKNYLDRMNNDGKKGVIDINIPFELQRSVYTQISDSTL
                     NEIWSFERSWIPHTSDTSKHIYYNPNGKYVKFLREFGEENKVIKNYYDSYVNTGDISP
                     SMVADLQMNKDVYDINDIRIQLFVAIHYLTLNDQLERKEIY"
     gene            complement(5141..6388)
                     /locus_tag="PKM34_02065"
     CDS             complement(5141..6388)
                     /locus_tag="PKM34_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNQ82397.1"
                     /translation="MSRNKGLYFILSVILILPQIIVAQAVNPNDAEKGWKPLFNGLDL
                     AGWRGFKADGIPADWSVADGCIVVAGTGGGESANDLITAEQYEDFDLYLEWAISPGGN
                     SGIFYHVLEGDYPSTYATGPEYQLIDDIGFPGKLEEWQQAGADYAMHTADKTRKKLMP
                     VGEFNSCRIRVENGHVTHWLNGEIILEFDLWTEEWHRLKNEGKWLDYPFYGLARKGHI
                     GLQHHGSLVRFRNIKIKDLTDPGAPLFNGNDLTGWVNHGNERWFVENGMIVGSSSPDG
                     GYGYLATDREYHDFILRLQFKLEEEGNSGVFFRSLLNGTDIKGWQAEVAPSGNNTGAI
                     YESGGRGWLNEIPEEREDILKPGEWNDMVISVKGDHVQTWLNGESMTDLFDDAVGKGQ
                     GVIALQVHSGGGVAVKWRDIFIR"
     gene            6527..8728
                     /locus_tag="PKM34_02070"
     CDS             6527..8728
                     /locus_tag="PKM34_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82398.1"
                     /translation="MESQKIVPHRLLLVLLGFTAMATQVILIREVLAIFQGNELVIGM
                     FLGIWMILTATGAYLSVQSSKFKVQSGFLKNKSTIDNRQSTIENPIAKSPNLLFLLAL
                     LPVASLFLLVTLRFSLVPAGIMPGLGKTILVFFLALLPFCMVSGLLFPKLVNSLSLLK
                     GKNLLHEGYALESAGGVVGGLLFSLVFILILPPYESLVLLSTFCLLVLFVIWLISGKI
                     VLAILALVTGAVVFLMPTVAGIARYLDRKQFSRQDVVEIRSSPFGLLAVSKMGNELII
                     YDNGSPVSLSADVIQREEAVHYAMLLHRSPKKVLLVSGGVSGAIDEILKYPVEKVDYV
                     EPNPWLIRLVDKYRPLPDDRRIRYIYKDPLIFLRNNPGTYDVILINAAEPHSAESNRY
                     STAEFYKLLKDKLNHGGIVSVATEASGNYMNETSQMIHSVNYNTFKSAFSYIRIIPGA
                     KDYFLVSDTTIEQSIFKHYQDKGIDNEYVNPFYIDEELLRMRSELIHKDLLPDAPVNS
                     DLKPFVFSLFLRHWLEKFKMNTWIIPLILVLLLILSLIFFGPLNLGLFAGGFTASGLE
                     FILLIWFQVIYGNVYQMTGVIFAVFMLGLVIGSLLLVGGIKKNTFKGYLMIQGGYVCL
                     SLLVALLMIIMASGSPGMLTIGLILFLVLLTGLLMGTQFSSSAHLRTTSIINSARESF
                     SADLAGSATGIVLVSVYVVPLLGLPVTALFLAGLNVLATGVMAWKREGSWQ"
     gene            8812..>9135
                     /locus_tag="PKM34_02075"
     CDS             8812..>9135
                     /locus_tag="PKM34_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ82399.1"
                     /translation="MQNIRNIAIIAHVDHGKTTLVDRILYQSHLFRDNEAMGELILDS
                     NDLERERGITILSKNVSVRYKGHKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQT"
BASE COUNT         2236 a         2391 c         2107 g         2401 t
ORIGIN      
        1 cctgctgatc atccatggaa caggcgacga caatgtccat taccagaatg ccgaaatgct
       61 gctgaacgaa ctggtgaaac acaacaagaa gtttcagttc atgccctatc ccaaccgcac
      121 acacggaatc tacgaagggg aaggcacttt gttgcacctg tcaacgcttt acaccgattt
      181 cttaaaatcg aactgccctc cgggagggag gtagatttgg tgtcaagaaa aaggattccc
      241 gctggcgcgg gaatgacgct caggctattt tgaccagtga ttgcgcgcgt tggctctcaa
      301 atgccagctc aaggatgcgg atgacattcc gtgcttcctg gggtgtaacg gcctgctccg
      361 cacctttggt gatcacatca aaaacattat cgtaaaaagc catataattt ccgggttccg
      421 tttcaatatt cccgtagaac tgctgcccgt gcatctctgc attcaacaat ccccagtaat
      481 cgggatcttc cttaccccag tcttccgaac ccggcattgc gccggctttc agcatctctt
      541 cctgcggatc gatgccgtat ttgatgaaag agccctttgt gccgtgaaga ctataccgtg
      601 gcccctgctc tttcacaaaa acggaggctt tcaggatcac tttgagccgc tcgtagtaaa
      661 gatgtacatg gaaataatcg tccaccttgc tctctgcccg ttgcatacgg atatcggcat
      721 aaaccgaccg cggcatcccg aacagcacca gcgcctggtc gaccaggtgc gagccgaggt
      781 catacagcac gccgccggcc ggcagcggct catcacgcca ggcgctgcgg cgcgcccccg
      841 gggcataccg gtcgaaatgc gcttcatatt cgaggatctc acctaaatat ccgttgtaaa
      901 ccacctgctg gatcgtttta aaatcgccat cccacctacg gttctggcaa acaaagatct
      961 tcttcccggt tttttcagct atcccgatca gtgcatcggc ttcctgagag gtaggagtga
     1021 aaggtttctc gaccaccaca tgcttgcccg cctccagggc tgacctggcc tgctcaaagt
     1081 gataaatatt gggcgttgcc aataccacca gttcgataga tccatcattc aatacattat
     1141 tatactcttt ggcaatcgta acgccagggt agatttcctt tgataatgaa gaatgccgct
     1201 cgacaacggt caccaggcgg aagcccggat gggtatggat aaagggagca tgaaataccc
     1261 ttcccgatag tccgaacccg acaatggctg tattaataat tccttctccc ataactattt
     1321 acaagatttt cttaagattc cggtagctga tcttcgtact ttccagcgag tcgcggttgc
     1381 atttgtcctg ttccacaaaa aaatatttca tcccggaaat atctttaaag ttcgtgaaat
     1441 aatccccata aggaatggtc ccttcgccga cttccgtaaa agtccgttcg gtcccggaat
     1501 ccatatcttt gatatgccat aactcaaacc ggccgggata tttgctgaga taatcccccg
     1561 gaactatgtt tgcatattgc atccagtaaa tgtcagcttc aaaacacacc aggtcaggat
     1621 cggtattctt taacaggatg tcgaaaccag tggtatcgcc gattttttta aattcaaaat
     1681 catgattatg atacccgaat ctaagcccgt ctttcttaca ggcttcgccc agccggttaa
     1741 acaagccggc agcccggaga taatcgtcgg ccgaagggat ctcgggcatt gaaatccagg
     1801 ggtaaacaac atacggcaca tccagttcga ggtgcgcgtc gatcacatgc cggctctgtt
     1861 ccggtgaaaa cgccacatgc gagctgaccg gctgcatgcc tgatccgctg acgagcgatc
     1921 taaactcgcc gggggccatc ccgtagaact ttccatcgtt ataaccggcc gcttcgagcc
     1981 gggtataacc gatcttcgct acagcctgaa gagttcccgg taagtctttg ctgatgtggt
     2041 ccctgacagt gtacagttgc aggccgatgt cctttttgtg cggggcagcc ctcaggattc
     2101 ccggcgccaa taaggcggaa gcggtagcca gggttgtggt cttaagaaaa tctcttctgg
     2161 ttatcataca aatagtaata gaacacggat gatacgaata aaacgaatta acacgataaa
     2221 ttatccaatt gactcatttc caaatctcca aatccccaaa tcctcaaagg atcttctccc
     2281 ccaccgggtc ccagaagacg cgccggtttt ccttgtacgc catcgtaccc atgtgggccg
     2341 tcatcgcctc ttcaaacgcc tggtcgatgt tgcagctggg ctggccgccg ttgcggatac
     2401 agtcgagcca ctcgcggata tgcaggtagg tggtatccac ccttttccct ccgcgatagg
     2461 tatacatcag cccgcgtccg gcaaaatact gttccgttgg cgatgtgata gcgtcaacat
     2521 tcttgaggcc cggagtatag gtcaggatgg gcagatcggg gtcgataacg ccgttgtcca
     2581 gcttctcccg gaagcgagtc gatccccggt cggcatagac tgtcatggat gaacccagtt
     2641 ccattgttcc gtcgtggccc atgatgaccc tgccgcggcg ccggttgctg gccagcgtgg
     2701 cgctgtacat cagtgtcagg tcgcggccgg ggtactcgaa gacggtgtgg agcacatcgg
     2761 ggactgtccg gccatcttta aaaaagtata ttccaccgct tgaaatggct gaatccggaa
     2821 tacccaggcc caggatctgg ttcatggcat cgtactcatg ggtcagcaga tcgcccgaca
     2881 ggccggtgct gtaatcccac cagcagcgcc agcggaagaa ccgctccagg ctgaagtcgt
     2941 gccagggtgc ctggccgatg aactgctccc agtcgatcgt ctgccggccg gccgtttcgt
     3001 ggatcggata gacccaggcg ccgttgggat cgttgcggtt ggtcgtcact tcgatcaggt
     3061 tgaccttgcc gaggattccc tggctgatca cctcttttgc tttcagataa ctttcattct
     3121 gacgcccctg gtggccgagc tggaagacga tgttgttttc cttgaccgct tttctgactt
     3181 cataggtctc ttccaccgtc catgtgagcg gcttttcgca ataaacgtgc tttccggccc
     3241 tggctgcggc aatggtcatc gggccgtgcc agtggtcggg cgtggcaatg atcaccgcat
     3301 cgacctcggg cgaattgatc aggtcgaggt ggttcaggaa ccgtttcggt tgttggccca
     3361 ttttgccacc cgtcccttcc ctgtgtacat tggatccggc ttccacggcc atattgccat
     3421 acgtgtcaaa aatatcacac accgccgtca cccgcacatt gagcggttcc tgctgctggt
     3481 attcttcata tcttttatcc tgccggttgt tcaccgcccc ctctttccag tcgtcgatca
     3541 tggtcgggtg ggcaaatccc gcggccagtg ccagatcttt gcctcgtatc ccgtaaccga
     3601 tcaatcccag cctgatctcc cggcccttgg aaggatcgcc ggtcagtgct gccggctccg
     3661 tatcgatctt cagctcctcc tggatattgt ctctgaggta tttttcgaat ttccttttcc
     3721 ggtaaaaagc atacgccagc gctcccagca cgggcacact caccatcgat ttcagcagat
     3781 ctcgtctttc catttaaccc gaataacagt atgtaacaac ctcaaaatac caaaatatca
     3841 caacttcaca aaatcaaaat atcacaacct caaaatacca aaatatcaca acttcacaaa
     3901 atcaaaatat cacaaaatca aaatatcaaa atatcctcaa tctcttctcc ttccaaccca
     3961 tctatccact ccccattctt tccatgtcgg gaacaacagc agcaccgcca gcgccagccc
     4021 ttcaatcacc atcttgttga ttaccaggta gctgccatcg ctgggcacgg cgtatttcaa
     4081 accgacaaac ggtgggtgcg acaggtagta aaaagccata agcacaatgc cggagataat
     4141 ggcagtccgt gtaaaaagac ccaggatcag tcccagcccg atcaggatca atccccagat
     4201 attcaggaga tcgacaattt tcagcatatc cggattggag gccaggctga tgaagaagcc
     4261 tttcaggaaa ccacccgagt cgagcaggta accggcactg gtccagttgg gattggccag
     4321 ttttacaagg ccttcgtaga ggaaatgcca gccgatggcc aggcggagaa ctactagtgt
     4381 gagcttctga gtgttcgtca tgagtttttg gttgatggtc gttggtcgtt ggtcattagt
     4441 cattaatcac ttataatgtg gttatttcaa tatatttctt ttctttccag ttggtcattc
     4501 aaagtcaggt aatggatggc cacaaacagt tgaattctta tatcatttat atcatataca
     4561 tctttgttca tttgaagatc agcgaccatc gacggagaaa tatcacccgt atttacatac
     4621 gagtcataat aattcttaat tactttattt tcttctccaa attctcttaa aaacttaaca
     4681 tacttcccat ttggattata ataaatatgt tttgaggtgt cggatgtatg cgggatccag
     4741 gatctttcaa aactccagat ttcatttaaa gttgaatcac tgatttgtgt gtaaactgac
     4801 ctttgaagtt caaaggggat attaatatca ataactcctt ttttcccatc attattcatc
     4861 ctgtcaagat agtttttaaa gcatttttga gtattagctg aatccattaa atgcttggaa
     4921 cagatttgat cagtaaaaaa atctaatatt attgataaat ctttgatttc agattggctg
     4981 aaaaccgaat tgataacagg atcggattgc aggtcagaat tacatggtcc tgagcaattt
     5041 aaaagcaaaa ggcctattaa taaatgaacc atattataaa attcggacat gactgacgac
     5101 taatctatcg gacatcgcac acatgacaag cgatcaattt ctaacgaata aatatatccc
     5161 tccatttaac agcaacacct cccccgctgt gcacttgcag agcaatgaca ccctgacctt
     5221 tgcccacggc atcgtcgaac aggtcggtca tggattcacc gttcagccag gtttgtacat
     5281 ggtcaccttt gacggatatc accatgtcgt tccattcccc gggcttcagg atgtcttctc
     5341 tttcttccgg aatctcatta agccaacccc ggccacccga ttcgtatatg gctccggtat
     5401 tatttcctga cggggccact tcggcctgcc accctttgat atcggtacca ttgagtaacg
     5461 accggaaaaa aacaccgcta ttaccctctt cctccagttt aaattgcagc ctgaggataa
     5521 aatcgtgata ttcccggtcg gtggcaaggt agccatagcc tccgtcaggg ctgctggatc
     5581 ctacaatcat cccgttttcg acgaaccatc tctcattgcc atgattgacc catcccgtaa
     5641 ggtcattccc gttaaaaagg ggggcaccgg gatcggtcag gtctttgatc ttaatattgc
     5701 gaaaacggac caggctgccg tggtgctgca acccgatatg gcctttccgg gcaagcccgt
     5761 aaaagggata atccagccat ttcccctcat tcttcaaccg gtgccattct tcggtccaca
     5821 ggtcgaactc aaggataatc tccccgttca gccagtgagt tacatgcccg ttttcaaccc
     5881 ggatgcggca tgaattgaat tccccaaccg gcatcagctt tttcctggtc ttatcggctg
     5941 tatgcatggc ataatcggcc ccggcctgct gccactcttc cagtttaccc gggaacccga
     6001 tatcgtcgat caactggtat tcaggcccgg tcgcataggt cgacgggtaa tccccttcca
     6061 ggacatggta aaagataccg ctgttgccac cgggagaaat ggcccattcc aggtaaaggt
     6121 cgaaatcctc atattgttcg gcggtgatca ggtcgttggc cgattctccc ccgcctgttc
     6181 ccgcaacgac gatgcatccg tctgcaacgg accagtcggc gggtatgcca tccgctttga
     6241 acccgcgcca gccggcgagg tcaaggccgt tgaacagggg tttccaaccc ttttcggcat
     6301 cattcgggtt aaccgcctga gcaacaataa tctgcggaag tattaaaata actgacaata
     6361 tgaagtataa tcctttattc ctgctcatag ttccatattt tcgaccgtta aaattaaaaa
     6421 tagaaattga gatggagtct cgattgaaaa caatttatct ttatgaggaa caatttgaaa
     6481 tgagatggag tctcgagatt tcgagatgga gtctcattga tttgagatgg agtctcaaaa
     6541 aatcgtcccc caccggctgc tcctggtcct cctcggattc accgctatgg ccacccaggt
     6601 catactcatc cgggaagtcc tggccatttt tcagggcaat gaattggtta tcggcatgtt
     6661 cctgggtata tggatgatac tgacagcaac cggagcctat ctttcagttc aaagttcaaa
     6721 gttcaaagtt caaagtggct tcctaaaaaa taaatcgaca atcgacaatc gacaatcgac
     6781 aatcgaaaat ccaatcgcca aatcgccaaa tcttctcttc ctcctcgctc ttctccctgt
     6841 cgcctctctt tttcttcttg taaccctccg cttctccctt gtcccggccg gtatcatgcc
     6901 cggcctgggc aaaaccatac tggtgttttt tctggccttg ttgccatttt gcatggtatc
     6961 cggtttatta tttccaaagc tggtaaacag cctttctttg ttgaagggaa aaaacctttt
     7021 gcatgaaggg tacgccctgg aatcggccgg gggcgttgtc ggtggcctgc ttttcagcct
     7081 ggttttcata ttgatcttac caccctacga gagcctggtt ctgctctcaa cattctgcct
     7141 cctggtcctg tttgtaatat ggctgatctc cggaaagatc gttttggcca tattagcgct
     7201 ggtaaccgga gcagtggtgt ttctcatgcc cacagtcgcc ggcattgcac gatacctgga
     7261 cagaaagcaa ttcagcaggc aggatgtcgt tgagatccgg tcgtcgccat ttgggttgct
     7321 ggcggtaagc aaaatgggaa acgagttgat catttacgat aacgggagcc ctgtcagttt
     7381 aagcgctgat gttatccaac gggaagaagc cgtgcactac gccatgctgc tgcatcgttc
     7441 gccgaaaaag gtcttactgg tttccggcgg cgtttcgggc gctatcgacg aaatcctgaa
     7501 gtatccggtt gaaaaggtcg attatgtgga gcccaatccc tggctgatcc ggctggttga
     7561 taaataccgg ccattgccgg atgaccggag gatccggtac atttataaag atcctttaat
     7621 ttttttgaga aataacccgg gcacctacga tgtgatcctt atcaacgcgg cggagcctca
     7681 ttcggccgaa tcgaaccgct attccacggc agagttctat aaattactga aagacaagct
     7741 caaccatggc gggatcgtct cagttgccac agaggcctcc ggtaattaca tgaacgaaac
     7801 ctctcagatg atccattcgg tgaactataa tacatttaaa tcagccttca gttacataag
     7861 gatcattccc ggcgcaaaag actacttcct ggtatcggat acaacaattg agcaatcgat
     7921 ctttaagcat tatcaggata aagggatcga taacgaatat gtcaatcctt tttacattga
     7981 tgaagagcta ctcagaatgc gcagcgagct gatccacaag gacctgttgc ccgatgcccc
     8041 ggtgaattcc gacctgaaac cctttgtctt ttcgttattc cttcgccact ggctcgaaaa
     8101 attcaaaatg aacacatgga tcatcccctt gattctggta ttgttattga tcttgtcatt
     8161 gatcttcttc ggccccctga acctgggcct gtttgccgga ggtttcaccg catccggcct
     8221 tgaattcatc ttactgatct ggttccaggt gatatatggc aatgtttacc agatgaccgg
     8281 ggtgatattt gcagtgttta tgctggggtt ggtcattggg tctttgttat tggttggtgg
     8341 tataaagaaa aataccttca aagggtacct gatgatacaa ggggggtatg tttgcctttc
     8401 tttactggtc gcattattaa tgataataat ggcttccgga tccccaggca tgttaacgat
     8461 cggtttgatt ttatttttgg ttctgctaac aggtctgctc atgggaaccc agttctcttc
     8521 aagtgcccat ctgaggacaa catccattat aaacagcgcc agagaatcct tcagtgccga
     8581 cttagccgga tcggcaacag ggattgtgct ggtatcggtt tatgtggtac cgttgctggg
     8641 attgccggtt accgcgctct tccttgccgg gctgaacgtg ctggcgaccg gggtgatggc
     8701 gtggaagcgt gaaggcagtt ggcagtaaat aataagaacc atatcaggac gcgagcgaag
     8761 cgagctaatg accattattt tccctacctt tgccggctat ttcaaaaatc catgcaaaac
     8821 atcagaaata tcgccattat cgcccatgtc gaccatggca aaactacgct ggtcgacaga
     8881 atcctctacc agtcacatct tttccgggat aatgaagcga tgggcgaact gatcctcgac
     8941 agcaacgacc tggaacgcga aagagggatc actatcctct ccaagaatgt ttcggtgcgt
     9001 tataaaggac ataagatcaa cattatagac actcccggcc actccgactt cggcggggag
     9061 gtggaacggg tgcttaacat ggcagatggc gtgctccttt tggtcgacgc atttgaaggg
     9121 ccgatgccgc aaacg
//
LOCUS       DAOQXS010000038         8892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8338_length_8892_cov_3.928822, whole genome shotgun sequence.
ACCESSION   DAOQXS010000038 DAOQXS010000000
VERSION     DAOQXS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8338_length_8892_cov_3.928822"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..696
                     /locus_tag="PKM34_02080"
     CDS             85..696
                     /locus_tag="PKM34_02080"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNQ82400.1"
                     /translation="MLIKIYDKNPSEKAIRQAAKVLEDGGLVIFPTDTIYAIGCSIYS
                     QRALERMARIKGVNPKNANFSFLFYDLSQLSEYTRPISNEVYKLMKRTLPGPYTFILE
                     ANSNVPKILGIKKKTVGIRIPDNGILLEIMRELGKPVMSTSVHDDDEVIEYTTDPELI
                     HERYEDLVDLVIDGGHGDNVPSTVYDCTGEEIVVVREGKGDIE"
     gene            746..818
                     /locus_tag="PKM34_02085"
     tRNA            746..818
                     /locus_tag="PKM34_02085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Lys,seq:ttt)
     gene            complement(863..2101)
                     /locus_tag="PKM34_02090"
     CDS             complement(863..2101)
                     /locus_tag="PKM34_02090"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ82401.1"
                     /translation="MKTFMLRFRTVLLLFLFSMIFVGANSQSSYLLVRGILKDSKTGE
                     AIRFAAITVPGTDIGTVSNSDGEFTLKISNVLKAEYFEVSHISYATSRFKISEATGEV
                     RTYLIELQPVQLKEVPVIDADARKIVEMAFRNIRQNYSTEPNMMTGFYREYIMQRRDY
                     LSISEAVVDIYNAPYQGFQNDQVKIFKGRKSSDVKNADTLMVRLQGGPYVLLLLDIVK
                     NTDLSIGLDDLDNYQYEISRYVQIDDKLHWVVDFVPAVIRENALYKGKLYISQENYAI
                     SRAEFSLDLSDEEKAASSFIKKKPSGMVFAPVSTGYIVTFKEQEGRYYLNYVRVDLKF
                     RCDWKKKLFRNTYTLMSEMAITGRNSGNIVKFGNEDQFRSSMIFSEKVTDFSDPDFWG
                     EHNIIEPEQSIENAIRKLMK"
     gene            complement(2264..4339)
                     /locus_tag="PKM34_02095"
     CDS             complement(2264..4339)
                     /locus_tag="PKM34_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNQ82402.1"
                     /translation="MKKIILLGISFLAISHLLAQDKMYIHFSDKMTLGAQVAETDSIF
                     FSDDETQAFFRIGDTLAQYPVSSIDSINFGANSDTVFVLYNGTDVSVINPMAFEGVTV
                     TVEGSDVTVNAVTEVQDINFCLSGTTGNGMFKIYTAKRYNLILNGVNLTNPDGPAINV
                     QTGKNTTVILAEGSTNTLADGTTYAEPPAGEDQDGAFFSEATLKFTGTGTLIINGHGT
                     DKHGLCSDDEIEVNEGTININCASGDGINGKEGVIISGGVIHITVNGDQSKGINCNST
                     LTLSGGDITVHNTGDAVLVQAGSGYDPSYCTAVKADGNIEINGATVTIVASGKAGRGI
                     SADTDIIISSGSVQVTSTGNGAVYTDSSGILDAYVATCFDSEGDIMITGGSVITSSSG
                     SGGKGINSGSELIIGTPESSPIIQITTTGTKVHISGGGQNSKDAEAKAMKIDTAVVIN
                     NGEITISSSDDAIKSENSITVNGGDIQVNNSYEGFEAPFITINDGNLHLYASNDGLNA
                     TFGSGGEGDDNSLLTINGGWVMLSVSNGDGLDSNGDIHITGGTTIVHGPQNAPEVGMD
                     YNGTCNMDGGFLVISGTNSFMTQAPDNSSDQHCVKVMMNQGLSNSTLFHIQDASGNNL
                     LTFQPARTYYSIVFSSSDLQTGQSYSIYTGGSCTGINVDGLYSGGVYSGGTFRKSFTV
                     NSIITNVNF"
     gene            complement(4336..4815)
                     /locus_tag="PKM34_02100"
     CDS             complement(4336..4815)
                     /locus_tag="PKM34_02100"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82403.1"
                     /translation="MMTKRIFFLILFLLAFKAMNAQKLVIRLQDTSENIETLNEIRKL
                     YFSGNDLKIDFHSGADDTYAVADIRKLYFTETVSVFENNPGTNYHLKVYPNPSDDYIL
                     VAGIPENILTIDIYRNDGQCVLKIGTESGNIKIDTRQLASGLYFINAGGFTSKFIKR"
     gene            complement(4919..5521)
                     /locus_tag="PKM34_02105"
     CDS             complement(4919..5521)
                     /locus_tag="PKM34_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82404.1"
                     /translation="MQNMKSASLLSILLAIPIFFGSLSASGQWAFNGNHIYNTNSGNV
                     GIGTNSPSTILYVSKNITEPTITVRNLGGNGGATFSMIDDASGANWKFKATLSGGFKI
                     RDQINSLDVIVIEPGGPANSIYISQSGSIGIGTSSPAGTALLDLSSNSKGLLIPRMTQ
                     VQIANIANPSGGLLVFCLDDDKFYAYTTSVNLWKEILMVP"
     gene            complement(5570..7411)
                     /locus_tag="PKM34_02110"
     CDS             complement(5570..7411)
                     /locus_tag="PKM34_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82405.1"
                     /translation="MKTHYIISGLTAAMLISFYPAIYGQWASSGNNIYTTNSGNTGIG
                     TNSPLSLLYVVKTSTEPTITISNSGGTGGATYAMIDDASGAAWKFKATLSGGFKIRDH
                     SAGNDVITIEPNSDANRICIKNSGNIGMGTASPVLSALLHLEATNKGLLLPRLTQTEV
                     NAISGPANSLMLFNTSSGKVSAYFASAGIWKNIVYGSGTISVFTCGNSLTINHVAGLV
                     APVTKTVNYGTVLTSLSGNSCCWITQNLGSDRQALAVNDATEQSAGWYWQFNRQQGYK
                     HDGTNRTPNTTWINPITEHAEWMTVNDPCSIELGVNWRIPTYSEWRNVIFNGGWTDWN
                     TSWNSVFKMHAAGNLNELTGNLTWRGIYGDYWSGSQGNDSDGGLLELGNGINATLFSY
                     SKANGFTVRCIEAPAVPAFSCGTGLLINHVAGSVSPVTKTVLYGTVSGVPGETGKCWI
                     TSNLGADQQATAVSDATEASAGWYWQFNRQQGYKHDGSAVTPAWTITGIMENFDWDAA
                     NDPCVIELGFGWHIPTITEWTNADAAGGWTDWNGPWNSLLKMHAGGYLNPSNGVLYER
                     GTHGYYWSRNQNPNQTFDGLPLYFFSTYCDTGVSLNKASGQSVRCVR"
     gene            complement(7431..8648)
                     /locus_tag="PKM34_02115"
     CDS             complement(7431..8648)
                     /locus_tag="PKM34_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82406.1"
                     /translation="MKKILILWIIPDLLLVTLVLPLSGQWAINGSHIYNTNSGNAGIG
                     TSTPASLLYVARNTTEPTVTIRNSGGSGGATFSMVDVASGANWKFKATLSGGFKIRDH
                     TNSLDVIVIEPGSFTNAIYIKNSGNMGISTGSPDPSALLDISSGDKGFQLPRLTQAEI
                     QSIQGPADGLIVYCTTDEKFYVFLSSANTWKEISYGSGTISPAGLPCGTPLAINHVAG
                     SVAPVNKSTVYGTVTNVPGELTKCWITSNLGADNQATYAGDATETAAGWYWQFNRLQG
                     YKHDGSQRTPNTTWIDPITENSDWTTSNDPCNIELGAPWRIPTNTEWTNVSTAGGWSN
                     WNGPWNSLLKMHAAGFLQSNDGSLTFRGSNGSYWSGTQSDNSGSFSPYFDNFSFYIGY
                     GVKSFGNTVRCVK"
BASE COUNT         2329 a         2151 c         1938 g         2474 t
ORIGIN      
        1 tgtaccttat ttattacaat ctatttactt ttcattattc actattcgtt tttcactatt
       61 ccttattatt agtaaatttg tcagatgctg atcaagatat acgataagaa ccccagtgag
      121 aaagcgatcc ggcaggcggc aaaggtgctg gaagacggcg gcctggtcat ctttccgacc
      181 gacacgatct acgccatcgg atgcagtata tacagccaga gagcgcttga acggatggcc
      241 cgaatcaaag gggtcaaccc gaaaaatgcg aacttctcct ttctttttta tgacctgagc
      301 caactatcgg agtacacccg tccgatcagc aatgaggtct acaaactgat gaaaaggaca
      361 ttgccaggcc cgtatacgtt tatcctcgag gccaacagca atgtgccgaa aatattgggc
      421 atcaagaaaa agacggtcgg tatccgaatc cccgataatg gcatattgct ggagatcatg
      481 cgggaactgg gcaaaccggt catgtccaca tcagtccacg acgacgacga agtgattgaa
      541 tataccaccg atccggaact gatccacgaa cggtacgaag atctggtgga cctggtcatc
      601 gacggggggc acggcgacaa tgtccccagt accgtttacg actgcaccgg tgaggaaatt
      661 gtagtggtca gggaaggaaa aggcgacatc gaataaaatt ttaaactttg aacaaaaaat
      721 tttctatctt tgcgccctca tttgagattc tgtagctcag ccggtagagc aacggccttt
      781 taagccgtgg gtcccgggtt cgaatcccgg caggatcacc ccaacattgc gcattgcaca
      841 ttttgcattg cgcatttttc atttacttca ttaacttcct gattgcattc tcgatcgatt
      901 gttccggctc gataatgtta tgttctcccc agaaatcggg atcggaaaag tctgttactt
      961 tttcggagaa tatcatcgat gacctgaact ggtcttcatt tccaaatttt acgatattgc
     1021 ccgaattccg accggtaatg gccatttccg acattaaggt gtacgtattt ctgaaaagct
     1081 tctttttcca gtcgcaccga aatttcaggt caaccctgac ataattaagg taatacctgc
     1141 cctcctgctc tttaaacgta acgatatatc ctgttgacac cggtgcaaag accatcccgg
     1201 aaggtttttt cttaataaaa gaggatgctg ccttttcctc atccgaaagg tcaaggctga
     1261 attcggcacg cgaaatggca taattttcct gcgaaatgta aagcttacct ttatataatg
     1321 cgttttctct gatcacggca ggaacaaaat caaccaccca atgcagtttg tcatcgattt
     1381 gaacataccg ggagatctca tattggtaat tatccagatc atccagtcct atactgagat
     1441 cggtattttt cacaatatcc agaagcagca gcacataggg gccaccctgc aggcggacca
     1501 tcagcgtatc ggcattcttc acatcggaac ttttccggcc tttgaagatc ttcacctggt
     1561 cgttctgaaa accctggtaa ggagcattat atatatcgac taccgcctcg gaaatcgaaa
     1621 gatagtccct ccgctgcatg atatattccc tgtaaaagcc cgtcatcatg tttggctcgg
     1681 tgctgtagtt ctgccggatg ttccggaaag ccatctccac gatcttcctg gcatcggcat
     1741 cgattacagg aacctctttt aactgaaccg gttgtaattc tattaagtag gtcctgacct
     1801 ctcccgttgc ctcagaaatt ttgaaccgcg atgtagcata gctgatatgc gaaacttcaa
     1861 aatattcggc ttttaaaaca ttgctgatct ttaaagtgaa ctcaccatcg gaattcgaaa
     1921 cggttccgat atctgtcccg ggaacggtaa tagccgcaaa gcgtatggct tcgccggtct
     1981 tgctgtcttt caggatacct cttaccagaa gataggaact ctggctgttt gccccaacaa
     2041 atatcatact gaataagaaa aggagaagaa cagtcctgaa tctaagcatg aaagttttca
     2101 tttcgagaag attagaagtt atcgaataat cgcaaggatt ttatgcttaa taccattcca
     2161 ggaagtacac cggcacaacc tgaaatgagt ggatgaaatt aatgattttt aagtgaatcc
     2221 cgtgaaaact ggctaaaata atatcaacat aaacaggtga agatcagaaa ttcacattgg
     2281 ttatgatact attcacagta aaagacttac ggaatgttcc cccggaataa acgcctccgc
     2341 tgtacaaacc atccacattg atccccgtgc atgatcctcc ggtataaatc gagtagcttt
     2401 gccctgtctg aagatccgat gacgaaaaaa cgatagagta ataggttcga gcaggttgaa
     2461 acgtcagtaa attgtttcct gaagcatcct gaatgtggaa taaagttgaa tttgagagtc
     2521 cctggttcat cataactttt acgcaatgct gatcggaaga attatccggg gcctgggtca
     2581 taaaggagtt agttccggaa atgaccagga aaccgccgtc catattgcaa gtaccattgt
     2641 aatccatacc cacttccgga gcgttctggg gcccgtgaac tatggtcgtg cccccggtga
     2701 tatggatatc gccattgctg tcgagtccat caccattgga aacactaagc attacccagc
     2761 ctccgttgat ggtcagaagg ctgttatcgt ctccttcgcc tcccgacccg aaagttgcat
     2821 tcaaaccgtc gttggaagca tacaggtgca ggttgccatc attaatggta ataaaaggag
     2881 cttcaaatcc ttcataggaa ttattgacct ggatatctcc cccattgacc gttatgctgt
     2941 tttccgattt gatcgcatca tcggaagaag aaattgttat ttccccgttg ttgatcacta
     3001 cagctgtatc gatcttcatc gcttttgcct ctgcatcctt tgaattctgg ccgcctcccg
     3061 aaatgtgcac tttcgtaccc gttgttgtaa tctggattat cggggaagat tccggtgtac
     3121 cgatgatcag ttcgcttcct gagttgatcc cttttccacc tgagcccgaa ctcgaggtaa
     3181 taaccgagcc gcctgtgatc atgatatcgc cttcagagtc aaaacaggtc gccacatagg
     3241 catccaggat gcctgaggag tctgtataaa cagccccatt cccggtactg gtcacctgca
     3301 cacttcctga tgaaataatg atatctgtat ccgcagatat tccccggccg gcttttcctg
     3361 aggcgacaat cgtaactgtt gctccgttaa tttcaatgtt gccatcggct ttaaccgccg
     3421 tgcaatacga cggatcatac cccgacccgg cttgtaccag cacagcatcg ccggtgttat
     3481 gaacggtgat gtctccgccg ctaagcgtaa gggtggaatt acaattaatt cccttcgact
     3541 ggtcaccgtt aacggtaata tggattaccc ccccggaaat gataactcct tctttaccat
     3601 tgatgccatc ccctgatgca cagttgatat taatggttcc ttcatttacc tctatttcat
     3661 catcactgca aaggccatgt ttatcggtac catgtccgtt gatgatcagt gtacctgttc
     3721 ctgtaaattt aagcgtggct tcgctgaaga aagcgccatc ctggtcttct ccggccggag
     3781 gctcagcata cgtcgtccca tcggccaggg tattggtaga gccctctgcg aggatcaccg
     3841 tggtattctt acctgtctgt acattgattg ccggtccatc cggattggtt agatttacgc
     3901 cgttgagaat caggttatac cgttttgcgg tataaatctt gaacatcccg ttgcctgttg
     3961 tccccgagag gcagaagttt atatcctgga cttccgtaac agcgtttact gtcacatcag
     4021 atccttcgac cgtcaccgta actccttcaa atgccatggg attgatcacc gatacatccg
     4081 tcccgttata taatacaaag accgtatcgg aattcgctcc aaaattaata ctgtcaatcg
     4141 aactaaccgg atactgagcc agtgtatcgc ctattctgaa aaacgcctgg gtttcgtcat
     4201 cgctgaaaaa tatactgtct gtctcagcaa cctgggcgcc caaagtcatt ttatccgaaa
     4261 aatggatata cattttatcc tgcgccaaca aatgagaaat agccaggaag cttattccca
     4321 ataagataat ctttttcatc tttttataaa cttcgacgta aaacctcctg cattgataaa
     4381 gtacagaccg ctggccaatt gacgggtatc tatcttgata ttacctgatt ctgtaccgat
     4441 ttttaaaaca cattggccat cattccggta aatatcaatc gtcaggatgt tttcaggaat
     4501 ccctgcaaca aggatgtaat catcggatgg atttggataa acctttaaat gataatttgt
     4561 tccgggatta ttttcaaaaa cagaaacggt ctccgtaaaa tagagctttc gtatatctgc
     4621 aaccgcataa gtgtcatccg ccccggaatg gaagtcaatt ttaagatcat tcccggaaaa
     4681 gtaaagtttc cggatttcat tcaatgtttc gatgttttca gatgtgtctt gcaaacggat
     4741 cacaagcttt tgcgcattca tagctttaaa agccagtaaa aaaaggatta gaaagaagat
     4801 tctttttgtc atcataacaa atgaaataat tttcaaaagt gcatttatta atgataacaa
     4861 cctcattttt gaggtaaaag ggatgaaaaa tgcggtgaac agggaaaact aaattctatc
     4921 aaggcaccat caggatctcc ttccacaggt tcacactggt tgtataggca taaaatttat
     4981 catcatccag gcaaaagacc agcagacccc ctgaaggatt ggcaatattt gcgatttgca
     5041 cctgcgtcat cctggggatc agcagtcctt tggaattcga agataaatcc agcagggcag
     5101 tgccggcggg cgaggatgtc ccgatcccga tgcttccgga ttggcttata tagattgaat
     5161 tggccggccc tcccggttca atcactatga cgtcaaggct gttgatctgg tccctgatct
     5221 taaacccgcc tgacagtgtc gctttgaatt tccagttggc tccgctggca tcatcgatca
     5281 tggaaaatgt cgcacctcca tttcctccca gattgcgcac cgttatagtc ggttctgtta
     5341 tattttttga aacatagaga atagtggacg ggctattggt gcctatcccg acgtttcccg
     5401 aattggtatt gtaaatatgg tttccgttaa atgcccactg acccgaggcg gataaactcc
     5461 cgaaaaatat gggtatggct aacagaattg ataataaact ggcgcttttc atgttttgca
     5521 tatgtattga attgtttttc atattcttaa gataaattta ttattagatc taccttacac
     5581 atctcaccga ctggccgctg gccttgttca ggctaactcc cgtatcacag tatgtagaaa
     5641 agaaatatag cggtaggccg tcgaaagtct ggtttggatt ctgattcctg ctccagtaat
     5701 aaccgtgtgt tccccgttcg tacagaacac cattggaagg attcaggtat ccgcctgcat
     5761 gcatctttag cagtgaattc catggtccgt tccaatctgt ccacccccct gcagcatccg
     5821 cgttggtcca ttcggtaatc gttgggatat gccatccgaa tcccagttcg ataacgcacg
     5881 ggtcatttgc tgcatcccag tcgaaatttt ccataatgcc ggttattgtc catgccgggg
     5941 taacagccga cccgtcgtgc ttatacccct gttgccggtt gaattgccaa taccagcctg
     6001 cagaagcttc agtagcatcg ctgacggctg tggcctgctg gtcggcgccc aggttgctgg
     6061 tgatccagca cttgcccgtt tcgccgggca ctccgctgac cgttccgtaa aggacggtct
     6121 tggttaccgg tgagacacta ccggcaacat ggttaatcaa taagcctgtg ccgcatgaga
     6181 aagcgggcac tgccggcgcc tcgatgcaac ggaccgtgaa accattcgct ttgctgtagc
     6241 tgaaaagagt tgcattaata ccgtttccca gttccagaag ccccccatcc gaatcattcc
     6301 cctgggaacc actccagtaa tcaccgtaaa taccccgcca ggtcaggttg ccggttagct
     6361 cgttaaggtt tccggcggca tgcattttaa ataccgaatt ccatgaggta ttccagtcag
     6421 tccagcctcc gttaaaaatt acgtttctcc attcgctgta tgtcggtatc cgccagttta
     6481 ctcccagttc tatggagcat gggtcattta cagtcatcca ctcggcgtgt tccgtaatgg
     6541 ggtttatcca tgttgtattc ggagtccggt tagttccatc atgtttatat ccctgttgcc
     6601 ggttaaattg ccaataccac cctgccgatt gttcggtggc atcattgact gccagcgcct
     6661 ggcggtcgga gcccaggttc tgcgtgatcc agcagcaact gttccctgac agggaggtca
     6721 gaactgtccc ataattgact gttttcgtga caggcgccac caatcctgca acgtgattga
     6781 tagtcagcga attgccgcag gtgaataccg aaatagttcc tgaaccatag acaatattct
     6841 tccagatgcc ggcactggca aaataagccg agaccttccc tgaagatgta ttgaataaca
     6901 tcaggctgtt tgccgggccg cttatggcat ttacctccgt ctgggttaac cttggcaata
     6961 atagcccctt gtttgtcgct tccaaatgca aaagtgctga caaaacaggc gaagcagtcc
     7021 ccatgccgat atttcctgaa tttttaatgc aaatccggtt cgcatcactg tttggctcaa
     7081 tggtgatcac atcgttgccg gcggaatggt cgcggatctt aaatccgccg gaaagggtgg
     7141 ccttgaattt ccaggcagca ccgctggcat catcaatcat tgcgtaagtt gcgccgcctg
     7201 tccctcccga attacttatg gttattgtcg gttccgtact ggttttgacc acatacagca
     7261 aactcagtgg cgaatttgtg ccaatgccgg tattgccgga gttggtagta tagatgttat
     7321 tgccgctgct ggcccattgg ccgtatatgg ccggataaaa cgaaataagc atcgcagccg
     7381 tcaatcctga aataatataa tgtgttttca taaccggtgt gatttaataa tcatttgaca
     7441 catctgaccg tatttccgaa tgatttgaca ccatagccga tatagaaact aaagttgtca
     7501 aaatatgggc tgaagcttcc ggagttgtca ctttgtgtgc cactccagta gcttccattg
     7561 gaccccctga atgtaagcga accatcgttg ctttggagaa aacctgctgc atgcattttt
     7621 agcagtgaat tccacggacc gttccagttt gaccagcctc ctgccgtgct gacgttggtc
     7681 cactcggtat tggtgggaat gcgccagggt gcacccagct caatattaca gggatcgttg
     7741 gaggtggtcc agtcagaatt ttcagtgatt ggatcgatcc aggtcgtatt tggcgtccgt
     7801 tgcgaaccgt catgtttata tccctgcagg cggttaaatt gccagtacca accggcagct
     7861 gtttcagtgg catctccggc atacgttgcc tggttatccg cgcccagatt actggtaatc
     7921 caacatttgg tcaactctcc cggcacattg gttactgtcc catatactgt cgacttgttc
     7981 actggggcca cactacccgc aacatggttt attgctaaag gagtgccgca gggcaatccg
     8041 gcaggtgata tggtcccgga cccgtaggaa atctctttcc atgtattggc actgctcagg
     8101 aagacataaa atttttcatc ggtggtgcaa tagacaatca aaccatcagc cggtccctga
     8161 atggattgaa tctcagcctg tgtaagacgg ggtagctgaa atcccttatc ccccgacgat
     8221 atatccagta aagctgaagg gtcgggcgat ccggtcgata tacccatatt accggaattc
     8281 tttatgtaga tagcattggt aaaactaccc ggttcgatca caattacgtc cagagaattc
     8341 gtatgatccc tgatcttgaa tccaccgctt agcgtggctt taaacttcca gttggccccg
     8401 cttgccacat ccaccattga aaatgtcgca ccacctgaac cgcctgagtt ccgtatcgta
     8461 acggttggct ccgtagtatt ccgtgcaaca tacagtagac tggcaggagt actggtaccg
     8521 atacctgcat tacccgaatt cgtattgtaa atatgactgc cattaatggc ccactgtccg
     8581 gataacggta aaaccaaagt taccaatagc aaatccggaa taatccaaag gattaatatc
     8641 tttttcatat gggtatagga tttatgaaga tgattattat catggcttca gaacagaccg
     8701 ctagaaaggt ctttcctaac tataagccat ttgaaaggaa tggtttatcg tgttaacaaa
     8761 taaccggtgc taatataatc aaatattggg ataaagtcaa taaaaaaaga ggccgtctca
     8821 aaaggctttt tattaaccac gaagttatac cgttaatttc ctaatataaa ttcaaaatta
     8881 ttttattttt ga
//
LOCUS       DAOQXS010000039         8885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8344_length_8885_cov_3.424009, whole genome shotgun sequence.
ACCESSION   DAOQXS010000039 DAOQXS010000000
VERSION     DAOQXS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8344_length_8885_cov_3.424009"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKM34_02120"
     CDS             <1..249
                     /locus_tag="PKM34_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNQ82407.1"
                     /translation="IAIRIVRDEFCRKMIEQLGKPVVSTSANLSGQPAPLVFSKIAPE
                     IKNGVDYIVNYNRDVMVRSKPSTIIRLLGEGEFEVLRK"
     gene            252..1040
                     /locus_tag="PKM34_02125"
     CDS             252..1040
                     /locus_tag="PKM34_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ82408.1"
                     /translation="MKARISIIQFRPELADPAKNIGNLDNRMDEAKGSQLIVLPELSN
                     SGYNFTDASQALSVAETLDGQGAYQRFIADKAKKLNAFIVSGICEKDGNDLYNSAILS
                     GPAGIIGSYRKMHLFMNEKDIFRPGNSALQVFEAGGLKIGMMICFDYLFPEPWGILAR
                     HGADIICHPSNLLTGNPHKCMPGLALMNRIYIATANRIGTEGSLTFNGKSFFTDPAGN
                     VPVIASPDQEEIICLDINTDISSDKMITKRNHVFTDRRPEMYAG"
     gene            1244..3217
                     /locus_tag="PKM34_02130"
     CDS             1244..3217
                     /locus_tag="PKM34_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ82409.1"
                     /translation="MSLGRQLDDKAPNYLKQALGWSYHAPYAGHDGIQLAIGQVFTVG
                     KDHLFPYYRDMLTAISAGLTAEEIILNGLSKKDDIASGGRHMSNHFAKPEWNIHNVSS
                     ATGNHTLHAVGVARAMKYYKSGGVAISSQGESSTSEGYVYEAINGASNEELPVIFVFQ
                     DNGYGISVPKEDQTANYYAATNFEGFKNLRIIYCNGLDVFSSMNAMAMAKKHVLETGK
                     PVIVHAHCVRMHSHSNSDRHELYRDDTEIDEARGRDPLQKFRKRLLFSEIFSEDELNA
                     LDEKAKKEISAAHKKVIMMPDPDPATIFDHVIPAPYIPVNFPEGLHQETGTPKKLIQA
                     LNDTLKEEFRLNPDTFIWGQDVANKEKGGVFNVTKGMQQEFGPDRVFNGPIAEDYIVG
                     TANGMSRFSDKIRIVIEGAEFADYFWPGMEQFVEMTHDYWRSNGQFVSNVTIRLASGG
                     YIGGGLYHSQSIEGALSTFPGIRIVYPSFADDAAGLLRTSIRSKGPTVFMEPKTLYND
                     PVAETFVPEGFEVPFGKARIRREGKDLSIITYGNTTHMSIQAAGKIEKELGKQVEVLD
                     LRSLIPLDKQAVLDSVKKTGRALIVHEDKVFAGFGGEIAAMITEEAFEFLDAPVKRVG
                     STFTPVGFNRILERAILPNSDKIYHAAAELLGY"
     gene            3290..4888
                     /locus_tag="PKM34_02135"
     CDS             3290..4888
                     /locus_tag="PKM34_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ82410.1"
                     /translation="MKKNFIAGLLLTAVLFLTATVSTGQNLANRKEIQKLIQTMQIIE
                     LAYVDSVDIQDIVTKTIEQVLKELDPHSAYLSAEELKKSNEQLEGSFEGIGVTFQIYH
                     DTILVISPVPGGPSEKLGILSGDKIVKINGEDATGKDINNEWVFDRLRGKKGTSVDIS
                     IYRGKKDLLDFTIVRDKIPLNSIDASFMATPEIGYIRLNRFSKTSQEEFVQSVTDLKS
                     KGMNKLILDLRGNSGGLLNTAFELADEFLPAGELIVYTEGLHNPRKDFYATSLGNFEK
                     GKLVIIIDEASASASEIVAGAVQDLDRGIVIGRRSFGKGLVQGPFDLSDGSLIRLTTA
                     RYYTPTGRSIQRPYENGTEDYYNDFSERFKHGEYVHADSIKFPDSLKYKTRAGRIVYG
                     GGGIMPDIFIPWDSTMFSDYFVELRRKNVLNTFTLDYVEDNRDKINRQYPGFREFNKE
                     FKITPDIIREFKETAEREGVKFDEEGWRASEALILAQIKAFIARNIWDVNAFYEVMSE
                     IDNEYHRAIDLLEDGQVFGILERN"
     gene            4944..5930
                     /locus_tag="PKM34_02140"
     CDS             4944..5930
                     /locus_tag="PKM34_02140"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNQ82411.1"
                     /translation="MADLSTSYMGLKLRNPIIAGSSGLTSTVDNIQKISENGAGAVVL
                     KSLFEEQINFAINKTFVQNENNFNYPEALDYISHYTRENDLEKYLELIREAKKSVDIP
                     VIASINCVSAAEWVTFAKNIQDAGADALELNIFVLPSDPRRKAEQHEQIYLDILQSVM
                     RQVRIPVAAKVSYYFSGLTNMALQLSWTGIKGLVLFNRFYSPDIDIEKFEVTSGHVFS
                     TEGESTLTLRWIAMLSDKVQCDLAASTGVHDGKGVIKQMLAGAKAVQVASVLYQKGFD
                     HIHVMTKEIEQWMARHDFKTTSDFIGRMSFKEVSNPAAYERVQFMKHFAGIE"
     gene            6196..6531
                     /locus_tag="PKM34_02145"
     CDS             6196..6531
                     /locus_tag="PKM34_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82412.1"
                     /translation="MKKRFIFLVIWIILIGLNSCKQNDPIQLDCDAHNYGFIYFQNNS
                     NDTYGIWIDGIFKMEVTGGTVSQAVNISEGNNRKLKAKQLDGYLIWPTEVETSFNVLS
                     CSSYTWQFP"
     gene            7135..7671
                     /locus_tag="PKM34_02150"
     CDS             7135..7671
                     /locus_tag="PKM34_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82413.1"
                     /translation="MKRRNVLILMGLFILIIGCTNSLTRSNAKKQILNEMKDEWKTFR
                     IAVSTRFTDGSVEEISTNINVVDLPESSISDLKRIEKILSDANVIVDNDYNPLIKPYI
                     DRDGNNRMWDFIPLGSAKKIEVDGIKTINDNYKKVEFSVFYEWNNLAKELGCTFPLVV
                     KRVIGFEKYDDGWRIDID"
     gene            8130..>8885
                     /locus_tag="PKM34_02155"
     CDS             8130..>8885
                     /locus_tag="PKM34_02155"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNQ82414.1"
                     /translation="MPHYQRLGLIPPKRHIQFRKPDGALYSEQLVSTEGFSAEYSLVY
                     HVYPPTVVTKIDKPFSVAPEIAVDKNMQHRSFQGFKVAPQDNYINSRIPVLVNDDCYI
                     HLAAPKSWAGQQSTRTMENGQSTIDNLIFFKNSDAHELIFIHQGEGTLKTMYGSIPFR
                     YGDHLIIPRGVIYRLDFNSEENRLFIVESFSPFRFPKRYVNRMGQLLEHAPFYERDIR
                     KPENLETHDAKGDFIVMVKKQDLVYPYHYASHPF"
BASE COUNT         2652 a         1927 c         2029 g         2277 t
ORIGIN      
        1 atcgctatcc ggatcgtacg cgacgaattt tgcaggaaaa tgatcgagca attgggaaag
       61 ccggttgttt ctacatccgc caatctctcc ggacaaccgg cgccactggt attcagtaaa
      121 atagctccgg aaattaaaaa tggagtggat tatattgtga attacaacag ggatgtaatg
      181 gtcagaagta aaccctcaac cattatccgc ttactcggtg aaggtgaatt tgaagtatta
      241 agaaaataac attgaaagcc aggatatcca ttattcaatt tcgccctgaa ctggcagacc
      301 ccgccaaaaa tatcggtaat ctcgataatc gcatggatga agcaaagggg agccaactga
      361 tcgttcttcc cgaattgtcg aattcaggat ataactttac tgatgccagc caggccctat
      421 ctgtggctga aacactggat ggacaaggcg catatcaacg gtttattgcc gataaagcca
      481 aaaaactgaa tgcattcatc gtcagtggca tatgtgagaa agacgggaac gatctttata
      541 actcggccat cttgtcaggg ccagcaggta tcattggcag ttaccggaaa atgcatctgt
      601 tcatgaatga aaaggatata ttccggcccg ggaattcagc attgcaggtt tttgaagccg
      661 gagggctaaa aatcggtatg atgatttgtt ttgactatct cttccccgaa ccctggggta
      721 tactggcccg ccatggcgct gatattattt gccacccttc caacctgctt acgggaaatc
      781 cccataaatg catgccgggc ctggccctga tgaaccggat atatatcgcc accgccaacc
      841 ggatcgggac agaaggatca ctcactttta acggcaaatc attttttacc gatcctgccg
      901 ggaatgttcc tgtaattgca tcccccgacc aggaggagat catttgcctg gatatcaaca
      961 cggatatttc atccgataaa atgatcacta aaagaaatca tgttttcacc gacagacggc
     1021 ctgaaatgta cgccggatag ttcatctcat atttagatca tttacaaata ataaccaatt
     1081 attgtgtaat tttgccgaac attccatccc tcggattgtt ataaaacaag tttaagtatc
     1141 tcatcgtatg tatcaaaata aacgattaag gatcggtaag gaagaaaagc tctattcaat
     1201 taaaaaagta agcagggaaa ctctccaaaa atggtattat ctgatgtcgc tcgggcggca
     1261 gttggacgac aaagccccga attacctgaa acaggcattg ggatggtcat atcatgcacc
     1321 atatgcaggg cacgacggca tccagctcgc tatcggccag gttttcaccg ttggtaaaga
     1381 ccatttgttc ccgtactaca gggatatgct gactgccata tctgccgggc tgactgcaga
     1441 agagatcatt ctcaacggcc tctcaaaaaa agacgatatt gccagcggag gccggcatat
     1501 gtcaaatcat tttgccaaac cggaatggaa tattcataat gtatcctccg caacgggaaa
     1561 tcacaccctg catgccgtcg gtgtggccag ggccatgaaa tattataaat cgggcggcgt
     1621 ggccatcagt tcgcaaggcg aatcttcgac ctccgaaggg tatgtttatg aagccattaa
     1681 cggggccagc aatgaagaac tgcccgtaat ctttgttttc caggataacg gctacggcat
     1741 atcggttccg aaagaagacc agactgccaa ttattacgcc gctaccaatt ttgaaggatt
     1801 taaaaatctc aggatcattt actgcaatgg attagatgta ttcagcagca tgaacgcgat
     1861 ggccatggcc aaaaaacatg tcctggaaac aggtaaaccg gttatcgttc atgcccattg
     1921 cgtcagaatg cattcccatt ccaattccga caggcatgaa ctctaccggg atgacacgga
     1981 aatagatgaa gcccgtggcc gtgatccgct ccagaagttc cgcaagagat tattattcag
     2041 tgagatattt tcggaagatg aactcaatgc actggatgag aaagcaaaaa aagagatttc
     2101 tgctgcccat aagaaagtga tcatgatgcc cgatcccgat ccggctacca tttttgatca
     2161 tgtgatcccg gcgccatata ttccggtaaa tttcccggaa gggctccacc aggaaaccgg
     2221 cactcccaaa aaactcatcc aggctctgaa cgataccctc aaagaagaat tccggctgaa
     2281 tcccgatacc tttatctggg gccaggacgt ggccaataaa gaaaaaggcg gggtgttcaa
     2341 tgtcacgaaa ggaatgcagc aggaattcgg ccccgacagg gttttcaacg ggcctatcgc
     2401 agaggattac attgtcggca cggcaaacgg aatgagccgt ttcagcgata aaatcaggat
     2461 tgtgatcgaa ggcgccgaat ttgccgacta tttctggccg ggcatggaac agttcgtgga
     2521 aatgacacac gactactggc gttcgaacgg ccagtttgtt tccaatgtga cgatcaggct
     2581 ggcttccggc ggttatatcg gcggtggact gtatcattct caatcaattg aaggagccct
     2641 ctccacattc ccgggaatcc gaattgtcta cccttcattt gccgatgacg ccgccggcct
     2701 gctccggacc agcatccgct caaaagggcc aaccgttttc atggaaccga aaacgctcta
     2761 taacgatcct gtggccgaaa ccttcgtgcc cgagggattt gaagtgcctt tcggtaaagc
     2821 ccggatcaga agggaaggga aggacctgag catcataact tacggaaata cgacgcatat
     2881 gtcgatacag gcggccggaa aaatagaaaa agaactgggt aaacaggttg aggttctcga
     2941 cctgcgttct ctcattccgc ttgataaaca ggctgtcctc gattctgtaa agaaaacggg
     3001 ccgcgcattg atcgtccatg aagataaagt ctttgccgga tttggcggag aaatcgcggc
     3061 gatgataacg gaagaggcat ttgaattcct tgacgcaccc gtaaaaaggg tcggatcgac
     3121 ctttacgcct gtcggcttta accggatcct ggaacgggcc attcttccca attccgataa
     3181 aatttaccat gccgccgcag aattgcttgg atattgataa agactaatta ataaggcgct
     3241 ttttatatta gattggctgt ttttccgttc ttattttaaa tacatgaaga tgaagaaaaa
     3301 ttttatcgcc ggattgcttt tgactgccgt tttattcctg acggccacgg tatcaacggg
     3361 acagaacctg gcgaaccgga aagagatcca gaaactgatc cagaccatgc agatcatcga
     3421 actggcttat gttgacagtg tggatatcca ggacattgtc accaaaacca tcgagcaggt
     3481 gctgaaagaa ctggatccgc actcggccta tctctcagcg gaagagctta aaaaatcaaa
     3541 tgagcagctt gaaggcagct tcgaagggat cggcgtgact ttccagattt accacgacac
     3601 tatcctggtg atctcaccgg tgcccggcgg tccgtccgaa aaactgggaa tcctgtcggg
     3661 tgataaaatc gttaaaataa atggagaaga cgccaccgga aaggatatca ataacgaatg
     3721 ggtttttgac cggctgcggg ggaaaaaagg cacctcggta gatatatcca tttaccgggg
     3781 aaagaaagat ttgctcgact tcaccattgt acgggataaa atcccactga attccatcga
     3841 cgcatccttt atggctacac cggaaatcgg ttatatccgg ttaaaccgtt tcagtaagac
     3901 atcccaggaa gagtttgttc aatccgtcac cgatctgaaa agcaaaggga tgaacaagct
     3961 gatcctcgac ctgaggggta attcaggcgg attgctcaac acggcctttg agctggccga
     4021 cgaattcctt cccgccgggg aactgatcgt ttacaccgaa gggctccata atccgcggaa
     4081 agatttttat gctacatcgc tgggaaattt tgaaaaaggc aagctggtga tcatcatcga
     4141 tgaagcatct gcttcagcca gcgaaatcgt agccggcgct gtgcaggatc tcgacagagg
     4201 catagtgatc ggcagaagat catttggaaa aggactcgtc cagggcccgt tcgacctgtc
     4261 cgacggttcc ctgatccgac tcaccacggc tcgttattac actccgacag gccgctctat
     4321 ccagcgccct tacgagaatg gaacggaaga ttactacaac gacttcagcg aacggttcaa
     4381 acatggcgaa tacgtgcatg ccgacagtat taaatttccc gattcactca aatataaaac
     4441 cagggccgga aggattgtat atggcggagg aggcataatg cccgatattt tcattccatg
     4501 ggattctact atgttttccg attattttgt cgaactgaga agaaagaatg tactgaacac
     4561 tttcaccctc gattatgtgg aagataaccg cgataagatc aatcgtcagt accccggctt
     4621 cagagagttc aataaagagt tcaaaattac ccccgacatt atccgcgagt tcaaggaaac
     4681 cgccgaaagg gaaggggtga aattcgacga agagggatgg agagcgtcag aagctttgat
     4741 cctggcgcag atcaaagcgt ttattgccag gaatatctgg gatgtcaacg cattctatga
     4801 agtgatgtcg gaaatcgaca atgaatacca ccgcgccatc gatttgctgg aagacggaca
     4861 ggtgttcggc atcctggaaa ggaattaata gattttactt gcctgataag attaatgatt
     4921 aaatttgctt caaaacttta atcatggccg atctctcaac atcctacatg ggtctgaaat
     4981 taaggaatcc catcattgcc ggaagttccg gattgacaag cacggttgat aatatacaaa
     5041 agatcagcga aaacggcgcc ggggctgtcg ttcttaaatc gctattcgaa gagcagatca
     5101 atttcgctat caacaagacc ttcgtccaga acgaaaacaa tttcaattat cctgaagcgc
     5161 tcgactacat cagccattat acccgggaaa atgacctgga aaagtacctg gaactgatcc
     5221 gggaggcaaa aaaaagtgtc gacattccgg taattgccag catcaactgc gtttctgcag
     5281 ccgaatgggt cacttttgcc aaaaatatcc aggatgccgg cgccgatgca ctggaactga
     5341 atattttcgt ccttccgtcc gatcctcgca ggaaagctga gcagcatgaa cagatctacc
     5401 ttgatatcct ccagtcggtg atgcgccagg tgaggatccc ggtagcagcc aaggtcagct
     5461 attatttctc cggcctcact aacatggctc tccagttgag ctggacaggc atcaaaggac
     5521 tggtgctctt taaccgtttt tattcgcccg atatcgatat cgaaaagttc gaagtgactt
     5581 ccggccatgt tttcagcacg gaaggggaga gtacgctgac cctgcgttgg atagccatgt
     5641 taagcgataa ggtccagtgc gatctcgctg cttccaccgg cgttcacgac ggcaaggggg
     5701 tcatcaaaca gatgctggcc ggggccaaag cggtccaggt agccagcgtc ctctaccaga
     5761 aaggatttga ccacattcat gtgatgacca aagagataga gcaatggatg gcccgtcacg
     5821 atttcaaaac cacttccgat ttcatcggcc ggatgagctt caaagaggtc agcaaccccg
     5881 ccgcctacga acgggtgcag ttcatgaagc actttgcagg gatagagtaa aatctcagca
     5941 acatcgagac cggggtgttg ggattttgga actttggaac gctttttcaa gttaaagagt
     6001 aaacggtaga ctgctgccac taaaacctga taggctatcg tgcttggtcg cattaacaga
     6061 gtttgttctt catcatatga cttcattctc tttaacctaa tactttccaa aactgattaa
     6121 tcagttatcc ttcattttct ttggaataat atttattttt ataccaagtt aataatccga
     6181 aaattagtgt tgaaaatgaa aaaacgattt atttttctgg ttatctggat aattctaatc
     6241 ggcctcaatt cctgtaaaca gaatgatccc attcaattag actgcgatgc tcacaattac
     6301 ggatttattt atttccagaa taactccaat gatacctacg ggatttggat tgatggcatt
     6361 tttaaaatgg aggttacagg cgggactgtg agccaggcag taaatatttc ggaaggcaac
     6421 aaccggaaat tgaaggcaaa acaacttgat ggttacctga tctggccgac agaagttgag
     6481 acgagtttca atgtactttc atgttcatca tatacatggc aatttccata aaaaaaactg
     6541 gctataaaga cggcggcgta acagtcggtg aaattaatcc tgctggctga ttttttttaa
     6601 gcgatagcgt aagactttgg tcactgtttc caggtgaaga gtgagcggtg aagcgtgaac
     6661 ggataaagga aaagggaaaa acgaaaaggg gaggggaaac aattctggaa ctgtgaaacc
     6721 aggaatcccg gaacgaacct ccctcccccg tgtgggggag ggttagggtg ggggctgaat
     6781 tcggatagcg gtagactttg gtcactgaaa ctccgtgtcg ttctgaattg ccgatccttt
     6841 agccggttta agcgatagcg taagaatttg gtcactaggc ttgggtaaat ggtgaattaa
     6901 aaaaggaatt gagaaaaagg aaaaggagcg agttaagatc tcagaaactt ttgaatcgtt
     6961 aaaataaaac cgaaaaccag attccgtttg ctaggctaag caagacttga ggcaatgaaa
     7021 cttatgtact actatattaa aaaaatatag ggttgagtat ttcttttaat ataaataaaa
     7081 attaatttta ctattattca tatgagagaa attagtcaaa ctaaaattat ttttatgaaa
     7141 agacgtaatg ttttgattct gatgggattg tttatcctaa ttattggctg caccaatagt
     7201 cttactagaa gtaatgctaa aaagcaaatt ctgaatgaaa tgaaagacga gtggaaaaca
     7261 tttagaattg cagtttcaac aagatttaca gatggatcgg tcgaagaaat tagtaccaat
     7321 attaatgttg tcgatcttcc agagagttca attagtgact tgaaaagaat tgaaaagatt
     7381 ctttcagatg ctaatgttat tgttgataat gattataatc ctctaattaa gccatatatt
     7441 gaccgtgatg gaaataatcg aatgtgggat tttattcctt tgggatcagc taagaaaatt
     7501 gaagttgatg gtattaaaac tattaatgat aattacaaaa aggtggagtt tagtgtattc
     7561 tatgaatgga ataatcttgc caaagaactt gggtgcactt ttccattagt agtaaaaaga
     7621 gtaataggat ttgaaaaata tgatgatggt tggcgtattg atattgatta attgggattg
     7681 aataataacg tcgaaattgt aattgaatta gtttaggtat cgggggttga tcgttcaatt
     7741 tgaatagttt ttcaccttaa tcatctttta tggtttccag caaacgatgt agttatgaga
     7801 agttcttcat aaatcatgtg aaaaaaacac caaacagaga tgttgaattc tttaacctca
     7861 ttataaaggt taactttact aaactagcaa taaaattatt gatataatca ctcattactt
     7921 tgagatcgct aacatgatgt atccatccta atcatgaatt atcgaattga gtaagatagc
     7981 catttaaatt ctgtcttact tgttgtaaat gagccccaat tcatgaaaag cgatacagta
     8041 acagtaaaga taaaggaagt aacccttaaa acccggaacc cggaaccaat tttttcctat
     8101 ctttgtaccc attctaaata agaaagatta tgcctcatta tcaaagacta ggactaattc
     8161 cgccgaaacg ccatatccag ttccgtaaac ccgacggggc tctctattca gagcagctgg
     8221 tctcaacaga aggcttttca gccgagtatt ccctggttta tcatgtatac ccgcccactg
     8281 ttgttacaaa aattgataag cccttttcgg tagcccctga gatcgctgtc gataaaaaca
     8341 tgcagcaccg cagcttccag ggatttaaag tagcacccca agataactat atcaacagcc
     8401 ggataccagt cctggtgaac gatgactgct atatccatct ggcagctccg aagtcgtggg
     8461 ccggtcagca atccacccgg acaatggaaa atggacaatc gacaatcgac aatcttatct
     8521 tctttaaaaa ctccgacgcc catgagctta tcttcatcca ccagggtgaa gggacgctga
     8581 aaaccatgta tggaagcatc cctttccgtt atggcgacca tctcatcatt ccgagaggag
     8641 tgatctaccg gctcgatttc aattcggaag aaaaccgcct gtttatcgtc gaatcgttca
     8701 gcccgttccg ttttccaaag cggtatgtga accgtatggg tcaactgctc gagcacgccc
     8761 cgttctatga gcgtgatatc cgcaaaccgg aaaatcttga aacccacgac gcaaaaggcg
     8821 atttcatcgt tatggtcaag aaacaggacc tggtttatcc gtatcattat gcttcccatc
     8881 ctttt
//
LOCUS       DAOQXS010000040         8793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8451_length_8793_cov_4.288510, whole genome shotgun sequence.
ACCESSION   DAOQXS010000040 DAOQXS010000000
VERSION     DAOQXS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8451_length_8793_cov_4.288510"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2588
                     /locus_tag="PKM34_02160"
     CDS             <1..2588
                     /locus_tag="PKM34_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82415.1"
                     /translation="NSDTSTIIVSHDIDLALKFADRIIVITKEEMGDIKPENVFYRGE
                     DHQKKSGPAKSYIFKSKIHKAFKDNPELIEGFKILVQDAVQQLENESKSEFKIKPGFF
                     QKMYSLFEAKEVRFSEIRNIKVNSLLRKILFLMQNPEKRSASIRGLNFFDETFFKELE
                     EILEAKGTHFAAKNGDELVKAEAQPWRRKIDSKEEALGDGQLRGLLSVLLKFFEREDK
                     LAVSGSTKSIYKNENSFFFFLFISISRLLSRIIKLKNKKLATPNADINNPDFARLFIR
                     NEGGILLGAGKKLTHFRTLFFALFVTFLGIGFATGSLSYLKDKMDDPFVNFVTTSVPS
                     EKQSEVASIIQHYNRESELKNQYKINVFTGFTIFPVEIYNKKSNDLSLITGRTITLND
                     PLLKELFKDKDNIITGNETGLFNDYDCGFIVSESLIKKLGMEPDARFLKVKILTTNAS
                     APDTASSGEIAFNEVTDNYELVPVPIRAILKKIPGDLNQDIDFIITEYAHLNLLDNHE
                     PSKEYPFHPSHTSSLMIYYNTADSAKAALMKDTLRSIVKSHPGITQGFKNRNFNLVRF
                     TEYIENVPFIVHSGYSENSDQQGELKAGNDSVTKSPTMTYAHPGFQIEFGFMGRKPDY
                     ERIQQMYDSVITILTGLNIDVSGLELLYKPNKRPPEMENTNPRLDRMMIHFNKLDKVE
                     EFAKFLETTKYKYLRMDMAKIMSMKNYNYVANLTTGIIIFLIALSVYFISYFVASVIA
                     GHLEKIKMNIGTLMAFGTSGLKFIYMNLISSYMLLSILVSFVAVFLAGSLWFCRAILW
                     LIYFFRKGLKTDFSLEPGTYFEIFNEWTLTAVVLIFVLSSIMSWFKIKKVLKSTPGDL
                     IFERE"
     gene            complement(2604..3713)
                     /locus_tag="PKM34_02165"
     CDS             complement(2604..3713)
                     /locus_tag="PKM34_02165"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNQ82416.1"
                     /translation="MRELSAPSGRLLSLDALRGFDMFWIIGAEGIVHALKEATGWGWA
                     VWMSGQLEHVEWNGFRFYDLIFPLFLFLSGATMPFSLTRKLEQGIPKNKIYLQVLKRG
                     LILVILGMIYNGLFRLDFENQRYASVLAQIGIAWMLAAFIFLNTSWKGQAIWVAGIVL
                     AYWAAMKLIPVPGFGPGVLTREGSLATYIDQMLLPGKMHEGIFDPEGIMVKIPATATA
                     LLGALTGWWLKNKSTGEYAKVGLMAAAAVFFFVIAYLIGLSFPINKKLWSSSFVFQTA
                     GCSVLLLALFYLLIDIWKFRKWAFFFIVIGLNPITIYMATGIINFWQISDYFLGGLAG
                     LSPEHWKNVWLITGMVAFEWLFLYFLYRKRILLRV"
     gene            complement(3710..5986)
                     /locus_tag="PKM34_02170"
     CDS             complement(3710..5986)
                     /locus_tag="PKM34_02170"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNQ82417.1"
                     /translation="MNAQIHALEATHYEISITDIDFQSGAITASTTVSLTPVSGPVDE
                     ISLELIDLAVGAVWTGNQPVSDFAQTDSLIIFPLPTALQPGDTLDVRIDYSGTPYHEG
                     WGGFHFAGEYAFNLGVGFEIIPHNLGKSWFPCIDNFTDRASYDVYCTLPAGKMAVGGG
                     ILAGISNNGNGTTTYYWKLSQQIPTYLASLAAGNYVAVSDTFSGMNGQIPIDIYVRPQ
                     DANYVAGTFANLKDILAIFEECMGPYSWDRVGYTSTLLGAMEHATNIFVPYGTINGSL
                     AYESLMAHELAHMWMGDKVTCSSAEEMWINEGWATFFGLYYALALNHDTNAFKQGMRA
                     NHAKVLQYCHTSSGDGSYFPLNQIPQEYTYGMSAYERGSTVAQALRFYLGDSLFFEAL
                     TGFIDEFAFDDASSYDMRDFLTGFTGMDMTGFFDNYVLNSGTPHYSIDSFDVSNSDVD
                     DYDVTVYVKQKRKGPAFTGNGNVMEIMFLDGNWNRYSDTIHFNGQSGCSVKSVPFLPS
                     VVLVDPEEKMCDATTDNYKIIKNSGNYSFDKTFFSLEQVTLIDSAFVQVTHNWAPPDS
                     IKDPDSGLRISDYRYWRIDGIFPDDFQARGKFFYSVGDLLDNNLILTSSDTVIILYRT
                     GAADDWHEITFDKIGPWNIGYIFVDDIQPGEYTLAVRETGVGIGSHTWEQAETLAVFP
                     NPSGGSFTISTVSDKGGELRIYSDSGTMVRSMTVHPGKKKTKWTPGNIAAGSYYVTFH
                     EAGRNTSESVTIIYSPGL"
     gene            6216..7559
                     /locus_tag="PKM34_02175"
     CDS             6216..7559
                     /locus_tag="PKM34_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ82418.1"
                     /translation="MKFFQKKRNLFLVIGGVILIIILVILAMNKGKDRTGTKVTAEKV
                     EKRTIIEMVSANGKIQPEKDIKISPYISGEVIELYVKEGDQVKKGDLLAKIDPEIYRA
                     NYDRMMAMLNSQKAGESNAQARVAQVEAQFRNAELNFNRSKTLWEQKVISDADFDAAK
                     SSYEVAKAEKDAAFQSLKSSQYGVESAEASLKEAKENLFKTTIISPSDGTVSKLSVEI
                     GERVTGASQFSAGTEIMRIANLQSMEVNVEVNENDIVRVSLFDTCQIEVDAYLDKKFK
                     GIVTEISTSANTSGVSADQITNFNVKIRILPESYTDMIDTASAIRSPFRPGMSATVDI
                     QTETVYNILAVPIQSVTTRSDTTGTVKEEDANIDLAATKDEVKEYIFLVKDGKALLQE
                     VKTGIQDITYIEVKSGVEEGQEVITAPYRAISKRLKNGDPIKVVDKKDLYSEEEK"
     gene            7606..8307
                     /gene="tsaB"
                     /locus_tag="PKM34_02180"
     CDS             7606..8307
                     /gene="tsaB"
                     /locus_tag="PKM34_02180"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNQ82419.1"
                     /translation="MPLILNLETATQVCSVALSDDGRVVELMESGEKNSHSVTLTVFI
                     DAVMKKAGIAFSELDAVAVSKGPGSYTGLRIGVSAAKGICYGAGKPLIAISTLESMAA
                     GAKGMASLGTPSLFCPMIDARRMEVYNALYDSDLNLVREIKAEIIHEGSFLEEMKDHP
                     VLFFGDGAEKCKPLLASHPNARFLDDFQLSATYMVHLAENIFNRRAFEDTVYFEPYYL
                     KDFIPGIPRVKGLNT"
     gene            complement(8354..8713)
                     /locus_tag="PKM34_02185"
     CDS             complement(8354..8713)
                     /locus_tag="PKM34_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNQ82420.1"
                     /translation="MKKIIALTGILVLAISISYPTMAQEKKKNYEEVQIQTSAICGMC
                     EDRIETNISFEKGVKKVQLNDDTKVVTIGYDPRKTAPDKLRTAISKLGYDADDKPADS
                     LAHAKLPVCCQKGSKPH"
BASE COUNT         2444 a         2055 c         2102 g         2192 t
ORIGIN      
        1 ctaactcgga tacatctacc attatcgttt ctcatgacat cgacctggcg ctgaagtttg
       61 ccgacaggat cattgtcatt acgaaagaag agatgggaga tatcaaaccc gagaatgtct
      121 tctacagggg agaagaccac cagaagaaat caggaccggc taaatcgtat atcttcaagt
      181 cgaagatcca taaagccttt aaggacaatc ccgaactgat cgaaggtttt aaaatcctgg
      241 ttcaggatgc cgtgcaacaa ctggagaatg aatcaaaatc ggaatttaaa ataaagcccg
      301 gcttttttca aaagatgtac tccctttttg aagcaaaaga agtcaggttt tccgagatcc
      361 ggaacattaa agtgaacagt cttctccgca agatactgtt cctgatgcag aatccggaaa
      421 aacggtcagc aagtatcagg gggctgaatt tcttcgatga aactttcttt aaagaactgg
      481 aagagatcct tgaagccaag gggacccatt ttgccgctaa aaatggtgat gaattggtta
      541 aagccgaggc tcagccgtgg agaaggaaga tcgattcaaa agaagaagca ttgggcgatg
      601 gtcagctcag aggcctgctg tcagtattgt taaagttctt tgaaagagag gataaactgg
      661 ccgtttcagg atcgaccaaa tcaatctata agaatgaaaa ttcttttttc tttttccttt
      721 ttatctcaat tagccggttg ctcagccgga tcattaaact gaaaaataaa aaactggcta
      781 cccctaatgc cgatataaat aatcccgatt ttgccaggct ctttatccgg aatgaaggag
      841 ggatcctact gggagccggc aagaaactga cccatttcag gacgcttttc tttgcgctgt
      901 ttgtgacatt cctgggtatc ggttttgcca ccggcagttt gagctatctg aaggataaaa
      961 tggatgaccc gtttgtgaac ttcgtcacca catccgtccc ctctgaaaaa caatccgagg
     1021 tggcttccat tatccagcat tataacaggg aatcggaact gaagaatcaa tataagatca
     1081 atgtttttac cggattcacg attttcccgg ttgagattta caataagaaa agcaatgacc
     1141 tctctttgat cacgggtcgg acgatcacac tcaacgatcc gctgttgaaa gagctattta
     1201 aggataagga taatattatt acagggaatg agacgggatt attcaatgat tatgattgcg
     1261 gatttattgt gagcgaatcc ctgattaaaa aactggggat ggaacccgat gcccggttct
     1321 taaaggtcaa gatattgaca actaacgcat ctgctcccga tacagcttca tccggtgaga
     1381 ttgcttttaa tgaagtcacg gataattacg aactggtgcc ggttcccatc cgggccatac
     1441 tcaagaagat cccgggcgat ttgaaccagg atatcgactt tatcatcacc gagtatgcac
     1501 acctgaacct gcttgacaat catgagcctt caaaagagta tcccttccat cccagccata
     1561 catcttcgct gatgatctac tacaatactg ctgactcagc caaggcagcc ctgatgaaag
     1621 atacgctgcg aagcattgtt aaatcccatc cggggattac gcagggattc aaaaaccgta
     1681 acttcaacct cgtccgtttt actgagtaca ttgagaatgt tccgtttatc gttcattccg
     1741 gatattcaga gaatagtgac cagcaagggg aattaaaagc cggcaacgat tcggtcacta
     1801 aatctccgac catgacttat gcccatccgg gtttccagat agagtttgga ttcatgggaa
     1861 gaaagcccga ttatgagcgg attcagcaga tgtacgattc ggttattacc attttgaccg
     1921 gtctcaatat tgatgtatca ggccttgaac tgttgtataa acccaacaaa cgcccgccgg
     1981 agatggaaaa taccaatccg aggctcgacc ggatgatgat ccatttcaat aaactcgata
     2041 aagtcgagga gttcgcaaaa ttccttgaaa cgacaaaata caagtacctg aggatggata
     2101 tggcgaagat catgtcgatg aaaaattata attacgtcgc gaaccttacc accggcatca
     2161 tcatcttcct gatcgcacta agcgtatatt ttatttctta tttcgtagcc agtgtgatag
     2221 ccggccatct ggaaaagatc aagatgaaca tcggaaccct gatggcattc ggtacaagtg
     2281 gattaaaatt catttatatg aacctgatca gttcctatat gcttttatcg attttagtct
     2341 catttgtcgc ggtgtttctg gcaggctcct tatggttctg ccgggctata ctctggctta
     2401 tctacttctt ccggaagggc ctgaagaccg atttcagcct cgagcccggc acatacttcg
     2461 aaatctttaa cgaatggacc ctgactgcag ttgttctgat tttcgttctg tcaagcatca
     2521 tgtcctggtt taagatcaag aaagtgttga aatctacccc gggtgatctg atattcgaac
     2581 gagaatagcg ggaagaactc cggctaaact cttaataata tccttttccg gtaaagaaaa
     2641 taaaggaaaa gccattcaaa ggctaccatt ccggtgatca accatacgtt cttccagtgc
     2701 tcaggcgata aacctgccag gccgcccagg aagtaatccg atatctgcca gaaattgatg
     2761 atcccggtag ccatgtatat cgtgatcggg ttcagcccga tgacgataaa gaagaatgcc
     2821 catttcctga atttccagat atcaatcaac aggtaaaata aggccagcag taacacgctg
     2881 cagcctgccg tctgaaaaac aaaagaggat gaccagagct tcttattgat tggaaatgaa
     2941 agtccgatga gatatgcaat gacgaaaaag aaaaccgcgg cagcagccat cagccccact
     3001 ttggcatatt caccggtgga tttgtttttg agccaccatc cggtcaatgc gccgagcaag
     3061 gccgttgccg tagccggtat ttttaccatg attccttccg ggtcgaagat cccttcatgc
     3121 attttccccg gcaacagcat ctggtcgata taggtggcca ggctgccttc gcgtgtcagc
     3181 acaccgggcc cgaatcccgg cacgggaata agtttcatgg ccgcccagta cgccagcaca
     3241 atccccgcaa cccatatcgc ctgtcctttc cagcttgtgt tcagaaatat aaaagcagcc
     3301 agcatccagg ctattccgat ctgtgccaga acgctggcgt agcgttggtt ctcgaagtca
     3361 agccggaaca gcccgttgta gatcatgccg aggatgacga gaatcaatcc tcttttcaat
     3421 acttgcagat agatcttgtt tttaggaata ccctgttcca gctttcttgt cagcgagaag
     3481 ggcatggtag cgccactcag gaataaaaac aacggaaaga tcagatcgta aaaccggaat
     3541 ccgttccatt ccacatgttc gagttgcccc gacatccaca ccgcccatcc ccagcctgtg
     3601 gcctctttca gggcatgcac gatcccttcg gcgccgatga tccagaacat atcgaagccc
     3661 cgaagggcat ccagcgaaag caatctcccg gaaggggcac tcaattctct cataatcccg
     3721 gtgagtagat aattgtaacg ctttcggatg tattccgccc ggcttcatgg aatgtaacat
     3781 agtaactgcc ggccgcaatg ttccccggcg tccattttgt cttcttcttg ccggggtgga
     3841 cggtcatcga cctgaccatc gttccggaat cgctgtagat tctcagttct ccgcctttat
     3901 cgctaactgt ggaaatggta aaactgccac cggaaggatt cggaaataca gccagggttt
     3961 ctgcctgttc ccatgtatgg ctcccaatac cgacaccggt ttccctgact gcaagcgtgt
     4021 attcgcccgg ctggatatca tcgacgaaaa tatacccgat gttccacggg ccgatcttgt
     4081 caaaggttat ctcgtgccag tcgtcggcag ccccggtccg gtaaaggatg atgaccgtat
     4141 cagacgatgt aagaatgagg ttgttgtcga gaaggtcacc caccgagtaa aagaacttgc
     4201 ccctggcctg gaaatcatcg gggaaaatac catcgatacg ccagtaacga taatcggaga
     4261 tgcgcaggcc ggagtcagga tccttaatac tgtcgggcgg cgcccagttg tgagtgacct
     4321 gcacaaaagc tgaatcaatg agggttacct gctccaggct gaagaaggtc ttgtcgaatg
     4381 agtaatttcc tgaattttta atgatcttgt agttatcggt agtagcatca cacattttct
     4441 cttccggatc aaccagcacg acagaaggca ggaacgggac cgatttgacg gaacagcccg
     4501 attgaccatt aaagtgaatg gtatcggaat aacggttcca gttcccgtcc aagaacatta
     4561 tttccatgac atttccattc ccagtgaatg ccggcccttt ccgtttctgt ttcacgtaaa
     4621 cggttacatc gtaatcatct acgtcactgt tggatacatc gaaggaatcg atggaataat
     4681 ggggcgtacc cgaattcaga acataattat cgaaaaatcc ggtcatatcc atcccggtga
     4741 aaccggtcag gaagtcgcgc atatcatagg atgaggcatc atcaaatgca aattcatcga
     4801 taaagccggt cagggcttca aagaacaggc tgtcgccgag gtaaaaccgc agcgcctggg
     4861 ccacggttga gcccctttcg taagccgaca tgccataggt gtattcctgg gggatctggt
     4921 tcaacgggaa gtaggaacca tcgccagagg aagtatgaca atactggagt actttggcat
     4981 ggttggcccg catgccctgt ttaaaggcat tggtatcgtg gttaagagcc agggcataat
     5041 agaggccgaa aaaggtcgcc catccttcgt ttatccacat ctcttccgcc gaagagcagg
     5101 tcactttatc gcccatccac atatgggcaa gctcgtgcgc catcagtgat tcataggcca
     5161 gagagccgtt gatggttcca tagggaacaa agatattggt ggcatgttcc atcgccccga
     5221 gcaatgtgga tgtgtacccc acccggtccc aggagtaagg tcccatgcac tcttcaaaga
     5281 ttgccagaat gtctttcagg ttagcaaagg tgccggcaac atagttagcg tcctgcggcc
     5341 tgacatagat atcgatgggg atttgcccgt tcataccgct gaaggtatcg gaaacagcca
     5401 cataattgcc ggcggcaagc gaagccaggt aggtcgggat ctgctgggac aacttccagt
     5461 aataggttgt ggtgccgttt ccattgtttg agatcccggc aaggatccct cctccgacag
     5521 ccatcttacc ggccggcagg gtgcaataga catcatagct ggccctgtcg gtgaaattgt
     5581 cgatacaggg aaaccagctc tttccaaggt tatgcgggat gatttcgaat ccgacgccca
     5641 ggttgaaagc atattctccg gcgaaatgaa agcctcccca accttcatgg tatggggttc
     5701 cggaataatc aatcctgaca tcgagggtat ctcccggctg aagcgctgtt ggcagtggaa
     5761 atatgatcag gctgtcggtc tgggcaaaat cggataccgg ctggttgccg gtccagacag
     5821 ctcccaccgc aaggtcgatc agttccaggc ttatttcatc aacgggcccg gagacaggcg
     5881 tcaggctcac ggtggtcgaa gcagtgatgg ctcccgactg gaaatcgata tccgttatgc
     5941 taatctcgta atgtgtggcc tcaagagcat ggatctgggc attcattgag agggaattca
     6001 gcatgataag acataaaacc ggcagcaagg atctggatgt tattctcatg ttattggaaa
     6061 taatgatgag tatcaaagat aaaagtatta tatcgaaaag aggatgaggt tggaaggaat
     6121 ttggatcatc atttataaaa atctaaaatg ccctttaaaa tctttaaaaa agccctactt
     6181 ttgatctttc ttgtgcatac cttaattcaa gcagtatgaa attcttccag aaaaaaagaa
     6241 atctcttcct ggtaatcgga ggggttatcc tgattattat cctggttatc ctggccatga
     6301 acaaaggaaa agacaggaca gggaccaagg tcaccgccga aaaagttgag aaacggacca
     6361 ttatcgagat ggtttcggca aatggcaaga tacagccgga gaaagacatt aagatcagcc
     6421 cgtacatctc gggtgaagtt attgagcttt atgtaaaaga gggcgaccag gtaaaaaaag
     6481 gcgacctgct ggccaagatt gatccggaga tttaccgggc caactacgac cggatgatgg
     6541 caatgctgaa ttcgcaaaaa gccggcgaat cgaatgccca ggcccgggtg gcccaggtgg
     6601 aggcgcaatt ccggaatgca gaacttaact tcaaccggag caaaacatta tgggagcaga
     6661 aagtgatctc cgatgctgat tttgatgcgg ccaaatcatc ttatgaagtt gccaaagcag
     6721 aaaaggatgc ggcattccag agcctgaaaa gttcgcagta cggtgtggaa agcgccgaag
     6781 cttcattaaa agaggcgaag gaaaatctgt tcaaaacgac aataatttca cccagcgacg
     6841 gaaccgtttc caaactgagt gtggagatcg gggaacgggt gacgggagct tcgcaatttt
     6901 cagccggtac cgagatcatg cgcattgcca acctgcagag catggaggtc aatgtggaag
     6961 taaatgagaa tgatattgtc agggtttcgc ttttcgacac ctgccagatc gaggtcgatg
     7021 cttacctgga caagaaattc aaaggcatcg tcacggagat atcaacgtcg gccaatactt
     7081 caggcgtcag cgccgaccag atcacgaact tcaatgtcaa gatccggatc cttcccgaat
     7141 cttataccga tatgatcgat accgcaagcg ccatccgctc tcctttccgg ccggggatgt
     7201 cggcaacggt ggatatccag accgagacgg tttacaacat tctggctgtt cccattcaat
     7261 cggtaacgac acgcagcgat acgacaggaa cggtgaagga agaggacgcc aatatcgact
     7321 tagcggccac taaagacgag gtaaaggaat atattttcct ggtaaaggat ggcaaagcgt
     7381 tgctgcagga ggttaaaacc gggatccagg atatcaccta catcgaagtg aaaagtggcg
     7441 tggaagaagg acaggaggtg atcaccgcgc cttaccgtgc catatccaaa agactgaaaa
     7501 acggtgatcc tataaaggta gtagataaga aggacctata ttcggaggag gagaagtaag
     7561 caactgtttc aatatcaatt tcaggaacat ttcacaaggg tagttatgcc gctcattctc
     7621 aatttagaaa cggcgacgca ggtgtgttcg gttgccttat ccgacgatgg ccgggtcgtt
     7681 gagctcatgg aatcggggga aaagaacagt cattccgtta ctttgactgt ttttatcgat
     7741 gcagtgatga agaaggcggg tattgcattt tccgaattgg atgccgttgc cgttagcaaa
     7801 gggcctggtt cttatacggg gctgaggatt ggagtatcgg cggcaaaggg aatatgttat
     7861 ggcgcaggca aaccattaat tgccataagt acactggaat cgatggctgc gggcgcaaaa
     7921 gggatggcgt cccttgggac gccatccctt ttctgcccca tgatcgacgc ccgccggatg
     7981 gaagtctata acgccctcta tgatagtgac ctgaatcttg tcagggagat taaggctgaa
     8041 atcattcacg aaggatcatt tcttgaagaa atgaaagatc acccggtact gttctttgga
     8101 gatggcgccg aaaaatgcaa accactcctg gccagccatc caaatgccag gttcctagat
     8161 gattttcagc tttcggccac ctacatggtc caccttgccg aaaatatatt caaccgacga
     8221 gcctttgaag ataccgttta ctttgagccc tattacctga aagattttat tccgggtata
     8281 ccacgagtga aaggtttgaa tacctgaccc ccggtagaat atctcccttc cccagctcaa
     8341 ccctcaaagc tctttagtgc ggcttgctcc ctttctggca gcaaaccggt aactttgcgt
     8401 gtgctaacga atctgccggt ttatcatccg catcataacc gagttttgaa atggctgtcc
     8461 gaagtttatc cggagcggtc ttccgcggat catagccgat tgtgaccact ttggtgtcat
     8521 catttaattg aactttctta acgcctttct caaacgaaat attggtctca atccggtctt
     8581 cacacatccc gcaaatcgcc gatgtctgga tctgaacttc ctcgtagttt ttcttctttt
     8641 cctgagccat agtgggatat gagatactaa tggctagaac caatattcct gttaatgcaa
     8701 ttattttttt cataatactt tgtttttaaa tggaatttat aacttatcat gttttagcaa
     8761 aaaccttacc cctgcatata ttttcactcc gct
//
LOCUS       DAOQXS010000041         8760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8496_length_8760_cov_3.401608, whole genome shotgun sequence.
ACCESSION   DAOQXS010000041 DAOQXS010000000
VERSION     DAOQXS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_8760_cov_3.401608"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..866)
                     /locus_tag="PKM34_02190"
     CDS             complement(111..866)
                     /locus_tag="PKM34_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ82421.1"
                     /translation="MLSKNQVKYIQSLRLGKFRDEYRVFIVEGVKMVDELLKSSFHIR
                     QILGTTSWIETHKDILKAKNPEVQVITEAELQRISHLVTPNEVLAVAGYPENDLPARE
                     GLGKIILVLDGIQDPGNLGTIIRTADWFGIRQVICSENTADIFNPKVVQATMGSIFRA
                     GIYYTVLKDFLEGLGPDWKIYGADTEGENIFGLEPGFPLAIVIGNESKGISPGLYPLL
                     TQKIGIPSGSTGAESLNAAVAAGIIMAVFGRKI"
     gene            complement(870..1739)
                     /locus_tag="PKM34_02195"
     CDS             complement(870..1739)
                     /locus_tag="PKM34_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNQ82422.1"
                     /translation="MKRGSVVLGVLFMLTVAMKSDKPAYIIFDVKGKQADYKDLLKSA
                     ADADIVFFGEMHDSPICHWLQYELTNDLFKEVNEDLILGAEMFETDNQLILDEYLAGK
                     IKEKNFEDEAKLWDNYKTDYKPLVKYALDKKLRFIATNVPRRYASLVNKSGFEGLDSL
                     TTEANAYLPPLPITYDPELEGYKSMMEMMEGAGGHASPNLPKAQALKDATMAHFILKN
                     IKKGDIFLHFNGTYHSNNFEGIVWYIKKERPDLKIITIASVEQDSPEELTEENSGLAD
                     FTICIPSTMSKSY"
     gene            complement(1943..2434)
                     /locus_tag="PKM34_02200"
     CDS             complement(1943..2434)
                     /locus_tag="PKM34_02200"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNQ82423.1"
                     /translation="MKKSEIRIIVEDYLSAEELPVQDRQLLAEAITGASTSYSPYSSF
                     RVGAAVRLDNGEIITASNQENAAYPSGLCAERVALFYAQANYPESSVTGIAIYAESAE
                     FTIERPVTPCGSCRQVMAEHEYRHGQKMRIIMGSSKGNVSVVEGIESLLPMQFVMEGL
                     RRE"
     gene            complement(2421..4136)
                     /locus_tag="PKM34_02205"
     CDS             complement(2421..4136)
                     /locus_tag="PKM34_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ82424.1"
                     /translation="MNSIRKSLVIFLSLLAFLPVISLSQAENNFEVSKNLDIYATLLK
                     ELDKNYAEAINPGELTQAAIDAMLETLDPYTVYIPESNAEDYKLMTTGQYGGIGALIH
                     KSGSYVVISEPYEGFPAQKAGLRAGDKILEINGKTMKDKNSDEVSEILKGQPGVELEL
                     LIGRINVPDPVRMKVVREEIKIPNIPYYGMLDDGIGYIKLTNFTQNAGGEVKKAFNEL
                     KTNNELKGLIIDLRGNGGGLLNEAVNISNIFVDQGQLIVSTKGKIAAKTQDHFTRSAA
                     IDKEIPLAVLVDKNSASASEILAGAMQDLDRGVVIGQRTFGKGLVQNVIPLTYNSQVK
                     ITVAKYYIPSGRCIQSIDYFHKDENGKSDKIPDSLISEFKTKNGRPVYDGGGIEPDIK
                     LPALKLSKISQALVTKYLIFDFANQYASKHATIPVAGEFEVTDEIYTEFTAFLQDKDY
                     DYKTASEEALEKLKSNAVREKYFDSIEEEYNALKGKILHDKSQDLITNKKEIKMLLKD
                     EIVARYYFQTGRVVASLNDDPEIEKAIEVLTGRDTYLAILDGSLKPDQKEKNTETDNG
                     SDEEE"
     gene            complement(4147..4569)
                     /gene="rnpA"
                     /locus_tag="PKM34_02210"
     CDS             complement(4147..4569)
                     /gene="rnpA"
                     /locus_tag="PKM34_02210"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ82425.1"
                     /translation="MSLFSFRKEEIIRKKKQIDRLFDEGRAFFIHPFKVIYLPYPAEP
                     DSPAQILISVSKRSFKNATDRNRIKRQIREVYRLNKHEFYHYLNEKQTGCLLAVIYSG
                     QVMHETHFLEKKIKAVINRLINELSKNLNNPSISLSDQ"
     gene            complement(4645..5394)
                     /locus_tag="PKM34_02215"
     CDS             complement(4645..5394)
                     /locus_tag="PKM34_02215"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNQ82426.1"
                     /translation="MRILVSQPQPADIEKSPYGDLTKKFNVDIEFFKFIKIEGIPSRE
                     FRQARVNLLDYSAVIFTSRNAVDHYFRMAKELRADIPQTMKYFCMSESTAFYLQKYVQ
                     FRKRKIFHGKQNFEELMEIIKKHKEEKYLLPCSDIHKASISGMLDEGEVNYTKAIFYR
                     TLASDLSHIDIKKYDMLIFFSPSGIASLMKNFPEFRQNSTIIGAFGPTTSQAVKDAGL
                     TLNIEAPTKTAPSMTMAIEEFLQKQTKKNHK"
     gene            complement(5453..6274)
                     /locus_tag="PKM34_02220"
     CDS             complement(5453..6274)
                     /locus_tag="PKM34_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNQ82427.1"
                     /translation="MTDPFTDNSIYSPGRSVCEYLDPGLWEACHASPSATVPEPFLYT
                     AKKNMIFQNLDIALNGSNWIFWILLAGFIALTFTRFYHERRLKLLAKSLFKHSAALQL
                     IRESPLHTHRSFLLFFLIYIISTSLLISQVSVIFSSGDNRTIGSLLFSLTAVGAYIGF
                     ILLKFLLIWIIGIIFKNIETAKEYVQNIIIYNFVQGMLLLPFLALIIYVHPTVFIYIS
                     IGIVLIMIVLRFTRGMMIGLSDPKFTLFHLFLYLCTLEILPLAFAAKWISKYFFS"
     gene            6402..6977
                     /locus_tag="PKM34_02225"
     CDS             6402..6977
                     /locus_tag="PKM34_02225"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNQ82428.1"
                     /translation="MFNLILFGPPGSGKGTQSVKIAEKYQLVHISTGDIFRREIKNQT
                     PLGIKVQSIIEKGELVPDELLVDILRSALHQAGSPAGFAFDGFPRTLRQAGDLDNLLA
                     ETHENVSLVLALEVDEEEVVKRLLKRARLEGRKDDTEDVIRNRMSVYHSQTHPLIDFY
                     EKQGKFVAIKGVGSVEEIFADICRAIDKVKT"
     gene            complement(6974..>8760)
                     /locus_tag="PKM34_02230"
     CDS             complement(6974..>8760)
                     /locus_tag="PKM34_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ82429.1"
                     /translation="GRNLAIITNAGGPGVMATDALISMDGKLTKLSDETMKKLNDYLP
                     PFWSHGNPVDVLGDAPPERFATATRIVLDDPNVDAVLVSLTPQAMTNPTATALEITKL
                     ADHTVKPIMAAWLGGESMREGISIFNKHGLSTYDTPEGAIRAFMTLASYSKNLDALFE
                     TPKDFPVSFTLDRDKVRKEFVEKHFTKERILSEDVSKALLTAYGIPTTQPMVAKSAIE
                     AADIANRIGYPVVMKIHSPDITHKSDMGGVILNIKDEDGVWNNFKNMTRSIKEKMPSA
                     NIEGVTIQPMVNMKDATEMILGIKKDPVFGTVLLVGMGGTGAEVFKDTKLAFPPLNER
                     LTRTMIKSLKIYPFLKGYRGAPEKDIEKLIEVIIRLSYLAADYPEIVELDINPLLVGT
                     SDVIALDARIVIDKELVGKDLPQFSHLLIHPYPDKYTKPAKLIDGNTILLRPIKPEDE
                     PLWLDLLGSCSKESIYSRFRYNFHYDSHEIATQFCFIDYAREIAIVAEVMEEGQKKLI
                     GVGRLIADLDHETVEYAVLIADAWQKKELGTILTEYCIEIARQWHLKRIAAETTKDNQ
                     AMISVFKKLGFKVVFNQDTGVSVSMDLV"
BASE COUNT         2160 a         2093 c         2012 g         2495 t
ORIGIN      
        1 ggaaccgacg gacttacccg tcggaccacc acattaaatc gaaaatgcgc aatgccgaat
       61 gccgaatcgg gcattttatt gctaacaaag aacttcctgc ccattactac ttaaattttc
      121 ctgccaaaca ccgccatgat gatccctgcc gcaacagccg cattcagcga ttcggcgccg
      181 gtagaaccgg agggaatacc tattttctgc gttaacaaag gatatagccc gggagagatc
      241 cctttcgatt cattgccgat tacgatggcc aaaggaaaac cgggctcaag gccgaaaatg
      301 ttctcgccct ccgtatcggc cccgtaaatc ttccaatcgg ggccaaggcc ttccaggaaa
      361 tctttcagga cggtatagta aatgcctgcc cggaatatcg atcccatcgt agcctgcacg
      421 accttgggat tgaaaatatc cgccgtattt tcagaacata tgacttgcct gatcccgaac
      481 cagtcggccg tccggattat cgttcccagg ttccccggat cctggatgcc gtcgaggacc
      541 agaataattt ttcccaggcc ttccctggcg ggaaggtcat tctcaggata accggccacg
      601 gcaagaactt cattcggcgt cactaggtgg ctgatcctct gcagctcagc ctcagtgata
      661 acctggactt caggattttt tgcttttaga atatctttat gggtttcgat ccaggaggtt
      721 gtcccgagaa tctggcggat atgaaaagag cttttcaata gctcatcgac catttttacc
      781 ccttccacaa taaaaacccg gtattcatcc ctgaacttcc ccaggcgaag ggattggata
      841 tattttacct ggtttttgga tagcatcttc tagtaggatt tactcatcgt cgaagggatg
      901 cagatagtaa aatctgcaag cccagagttt tcttctgtca actcctccgg cgaatcctgc
      961 tccactgagg ctatggtaat gattttcagg tcaggacgtt ctttctttat ataccagaca
     1021 atgccttcga agttattgga atgataagtc ccgttaaaat gcaggaaaat atctcctttt
     1081 ttaatgttct tcaggataaa atgagccatg gtcgcatctt tcagcgcctg ggctttgggc
     1141 aggttcggtg aagcatgccc gccggctcct tccatcatct ccatcatgct tttataccct
     1201 tccagttctg gatcataagt tattggaagg ggaggaagat acgcattagc ttctgttgtc
     1261 agactgtcaa gcccttcaaa acccgatttg ttgaccagcg aagcatagcg ccgggggaca
     1321 ttcgtggcta taaaacggag ctttttatcc agggcatact tgaccagggg cttgtaatcg
     1381 gtcttgtaat tatcccagag tttggcttca tcctcaaaat tcttctcctt aatttttccg
     1441 gccaggtatt catcgaggat cagctgattg tcggtctcaa acatttcagc ccccaggatc
     1501 agatcttcat tgacctcttt gaataaatca tttgtgagtt catattgcaa ccaatggcat
     1561 atcggggaat cgtgcatctc accgaaaaat acaatatcgg catctgcggc actcttcaat
     1621 aaatccttgt aatcagcctg tttcccttta acatcaaaaa taatatacgc cggtttatcc
     1681 gatttcatcg ccaccgtcag cataaacaaa actccaagta ctacacttcc tcttttcata
     1741 ttaacctaat attatcatta actttcccca gttcccagtt ccctgtccct gttcactgtt
     1801 caccgttcac tgttcactgt tcactgttca ctgttcactg ttcactgttc accgttcact
     1861 gttcactgtt ccctgttcac tgttcaccgt tcaccgttca ctgctcactg ttccctgttc
     1921 accgttcact gttcactgtt cactattccc tgcgcaaccc ttccatcacg aactgcatcg
     1981 gcaacaggga ctcgatgcct tcaactacgc tgacgtttcc cttgctgctt cccatgatga
     2041 tccgcatttt ctgaccatgg cggtactcgt gttcggccat gacctgccgg catgatccac
     2101 agggtgtaac cggccgttca atggtgaact cagctgattc ggcataaata gcgataccgg
     2161 tcacactgga ttcagggtaa ttggcctggg catagaagag ggcgacacgt tcggcgcaaa
     2221 gccctgaagg ataagctgca ttctcctggt tgctggctgt gataatctca ccgttatcca
     2281 gtcttacggc agctccgacc ctgaatgaag aatacgggga gtaagaggtg gaagctcctg
     2341 ttatggcctc tgccagcaat tgtctgtctt gaacaggcaa ctcttcagcc gacaggtaat
     2401 cttccacgat gatcctgatc tcactcttct tcatcgctgc cgttgtcggt ttctgtgttt
     2461 ttttccttct gatccggctt caaacttccg tcgaggatcg ccaggtaggt gtcccttccg
     2521 gtcaatactt caatggcttt ttcaatttcc ggatcgtcgt tgagagaagc aactacccgg
     2581 ccggtttgaa aataataacg ggcaacaatc tcatctttca gcaacatttt gatctccttc
     2641 ttattcgtga tcaaatcctg ggatttgtcg tgcaggatct tgcctttaag agcattgtac
     2701 tcttcttcga ttgaatcgaa gtatttttcc ctgaccgcat ttgacttcag tttttccagg
     2761 gcttcttcgc tggcggtctt atagtcgtaa tccttatcct gaagaaaagc cgtgaattcg
     2821 gtatatattt cgtcagttac ttcgaattcg ccggcaaccg ggatcgttgc atgttttgaa
     2881 gcatattgat ttgcaaaatc aaaaatcagg tattttgtga ccagggcctg tgatattttg
     2941 ctgagcttta gtgcaggcaa tttaatatcg ggttcgatgc cgccgccgtc atatacaggc
     3001 cttccattct ttgttttgaa ctcggaaatc agtgagtcag gaattttatc cgacttcccg
     3061 ttttcatctt tatggaaata atcgatcgat tgaatgcaac ggccgctggg aatataatat
     3121 ttggctacgg taattttaac ctgcgaattg taggtgagcg gtataacgtt ctgtaccaat
     3181 cctttgccaa aggtgcgctg accgatgacc actccccggt ccaggtcctg catggcgccg
     3241 gcgaggatct ctgaagcgga tgcgctgttt ttgtctacca gcaccgccag cgggatttct
     3301 ttatcgatgg cagccgaccg ggtaaaatga tcctgggtct tggctgcaat tttacctttt
     3361 gtgctgacga tcaattgccc ctggtcgaca aaaatgttgc tgatattgac agcttcgttc
     3421 aataaaccgc ccccattgcc cctcaggtcg atgatcaggc ctttaagctc attattggtt
     3481 ttcagctcgt taaaggcctt tttgacctca ccccccgcat tctgggtaaa attggtcagt
     3541 ttaatataac cgatgccgtc gtcgagcatc ccgtaataag gaatattcgg tatctttatc
     3601 tcttccctga caaccttcat cctgaccgga tccggtacat taatccggcc aatgagtaat
     3661 tccagttcca cgccgggctg cccttttaaa atttcgctga cctcatcgga gttcttatcc
     3721 ttcattgttt tgccattgat ctccaggatc ttgtctccag cgcgcaaccc ggccttctgg
     3781 gcagggaatc cttcataagg ctccgaaatg acgacataac tgccgctttt atggatcaaa
     3841 gcgccgatgc cgccatattg cccggtagtc atcagtttat aatcttcggc attcgattcc
     3901 ggaatatata ccgtgtaagg atcaagggtt tccaacatgg catcgatggc tgcctgtgtc
     3961 agttcgccag gatttatcgc ctctgcataa ttcttgtcaa gctctttcag taaagttgca
     4021 taaatatcca ggttcttgga tacttcgaaa ttgttctctg cctggctaag agaaataacc
     4081 ggtaaaaaag ccagcagcga caggaatatt accaggcttt tacgaatact gttcataact
     4141 gcatatttat tgatcagaca aagatatgga aggattgttc agattttttg aaagttcatt
     4201 gattaaacgg tttattacgg cttttatttt tttctcaaga aaatgagttt cgtgcatgac
     4261 ctgtcctgaa taaataacag ccaaaaggca tccggtctgt ttttcattca gatagtgata
     4321 aaattcatgt ttattgagtc ggtatacttc ccggatctgc cttttgatcc ggttccggtc
     4381 agtggcattt ttaaacgacc ttttgctgac gctgatcagt atttgggccg ggctatccgg
     4441 ttctgccgga taaggcagat agattacttt aaaaggatgt ataaaaaaag ccctcccttc
     4501 gtcaaatagc cgatctattt gctttttctt gcggatgatc tcctcttttc tgaaagagaa
     4561 aagcgacata agcaggatga aatttaacgg taacgcaatt caggggaggt gaggcagttt
     4621 acctcccgat gaacggcacg acggttattt atgattcttc tttgtttgtt tctgaagaaa
     4681 ttcttcaatt gccatggtca tcgacggggc ggtcttggtc ggcgcttcga tgttcagcgt
     4741 caatccggca tccttgacag cctggctggt ggtaggcccg aatgcgccga tgatggtact
     4801 gttctgcctg aattcaggaa agtttttcat cagcgatgca atacccgacg ggctgaagaa
     4861 aatcagcatg tcatattttt taatatcgat atgcgataag tcgctggcca gggtccggta
     4921 aaaaatagct ttggtgtagt tgacctcacc ttcatcgagc ataccggaga tactggcctt
     4981 gtgtatgtcg gagcagggca ggagatactt ttcctcttta tgttttttaa tgatttccat
     5041 gagttcctca aaattctgct tgccatggaa gattttcctt ttgcggaact gaacatattt
     5101 ctgaagataa aacgcagttg actcgctcat acagaaatac ttcattgtct gcgggatatc
     5161 cgctctgagc tcttttgcca tacgaaaata atgatctacg gcattacggc tggtaaagat
     5221 aacagctgag taatcgagca ggttgaccct tgcctggcga aattccctgg aggggatacc
     5281 ttcaatcttg atgaacttga aaaattcaat gtcaacgttg aacttcttgg tcaggtcgcc
     5341 gtatggtgat ttctcaatgt cggcaggctg cggttgtgat accaggattc gcatttattt
     5401 cttaaagtat cagttcgtta attaaaaatc catcaacggc aaccattttt tctcaggaga
     5461 aaaaatattt gcttatccat tttgcagcaa aagcaagcgg caaaatttcg agggtgcaaa
     5521 ggtacaaaaa taaatgaaac aacgtaaact taggatccga taaaccgatc atcattcctc
     5581 tggtgaatct taaaacaatc attataagca ctataccgat gctgatataa ataaaaactg
     5641 tcggatggac atagatgatc agggcaagaa acggaagtaa cagcattccc tgtacaaaat
     5701 tataaatgat gatattctga acatattcct ttgccgtttc gatgttctta aatataatcc
     5761 cgattatcca gatgagcaaa aacttcagaa gaataaagcc gatgtaagcc cccacggctg
     5821 ttaacgaaaa caagagcgac ccaatagtgc ggttgtcgcc cgaggagaat atgacagaaa
     5881 cctggctgat cagtaagctg gtggaaatga tgtaaatcag gaaaaacaat agaaaactgc
     5941 ggtgcgtatg cagggggctc tcacggatca attgaagggc tgcggaatgt ttgaataaag
     6001 atttagccag gagttttaag cgcctttcgt gatagaaccg ggtaaatgtc agggcgataa
     6061 acccggccag taatatccag aatatccagt tgcttccgtt taaagcaata tccaggttct
     6121 gaaagatcat attcttttta gccgtgtaga gaaatggctc cggaactgtt gctgacggcg
     6181 aagcatggca cgcctcccat aatcccggat caagatattc gcagaccgat ctgccgggtg
     6241 aataaataga attatctgtg aaggggtctg tcaagaattg gtattttggg caaaattaac
     6301 caaaacaatg gcaggaataa aaacgcttca ctatatttgt aaacgcggtt acccaaccgc
     6361 cactttcgca aaaaatcagt aaaaggctaa cgactaataa tatgttcaat ctgattctat
     6421 tcggtcctcc gggttcgggc aaaggaaccc agtcggtgaa aatcgctgaa aagtaccaat
     6481 tggttcatat ctcgacgggt gatatctttc gccgggagat caaaaatcaa accccacttg
     6541 gtatcaaagt gcagagcatc attgaaaaag gggaactggt tcccgatgaa ctgctggtgg
     6601 atatcctccg ttcggctttg catcaggcag gttcgcctgc cggctttgcc ttcgatggat
     6661 tcccgcgtac cttgcgccag gccggcgacc tggataacct gctcgctgaa acccatgaaa
     6721 acgtctccct cgtcctggct ctggaagtcg atgaggaaga agtcgtcaaa aggctgttga
     6781 aacgggcccg gcttgaaggc cgcaaagacg atacggaaga tgtgatccgg aacaggatgt
     6841 cggtctacca ttctcaaact cacccgctaa tcgatttcta cgaaaagcag ggcaagtttg
     6901 tcgctataaa aggggttggc tctgtcgaag aaatttttgc cgatatctgc cgggccatcg
     6961 ataaggtgaa gacttaaacc aggtccatcg atacacttac accggtatcc tggttaaaga
     7021 ctaccttgaa tcccagtttt ttgaatactg agatcatggc ctggttatct tttgtcgttt
     7081 cagcggcgat tcgtttcaga tgccattgac gggcaatctc aatgcaatac tccgtcagga
     7141 tggttcccaa ttctttcttc tgccaggcat cagcgatcag tacggcatat tcaaccgttt
     7201 catggtcaag atcagctatc aggcggccga ctccgatcaa ctttttctgc ccttcttcca
     7261 ttacttccgc cacgatcgca atctcccttg cataatctat aaagcagaac tgggtggcaa
     7321 tttcatggga atcataatgg aaattataac ggaaccttga atagatcgac tccttggagc
     7381 agctacccag cagatcgagc cacagcggtt cgtcttccgg tttgatcggc cgtaagagaa
     7441 tggtgttgcc gtcgatcagt tttgccggtt tggtatattt atccggatag gggtgaataa
     7501 gcaggtgcga gaactgggga aggtctttgc cgacgagctc tttatcgatg acgatgcggg
     7561 cgtcgagtgc aatgacatcg ctggtgccta ccagcaacgg gttgatatcg agctcgacga
     7621 tctcaggata atcggcggcc agataagata accggatgat cacttcgatc agtttttcga
     7681 tgtctttttc cggtgcgcca cggtatcctt tcaggaaagg atatattttc agcgatttga
     7741 tcatggtgcg cgtaagccgt tcattaagtg gcggaaatgc cagcttggta tctttgaaga
     7801 cttccgcgcc ggtgccgccc atccctacca ggaggacggt accaaaaacc ggatctttct
     7861 tgatcccgag gatcatttcc gtggcatcct tcatattgac catcggctgg atggtaaccc
     7921 cttcgatatt ggccgagggc atcttttcct ttatgcttcg ggtcatattc ttgaagttgt
     7981 tccaaacgcc gtcttcgtcc ttgatattca ggatgacgcc acccatatcc gatttatgtg
     8041 taatatcggg tgagtgaatt ttcatgacaa cgggatagcc gatccggttg gcgatatcgg
     8101 ccgcttcaat agccgatttg gcgaccatgg gctgagtggt gggtatgcca taggccgtca
     8161 ggagcgcttt tgaaacgtct tccgacagga ttctctcttt ggtgaaatgc ttttcgacaa
     8221 attctttcct caccttgtcc cggtcaaggg taaacgaaac agggaaatcc ttcggtgttt
     8281 caaacagggc atccaggttt ttcgaatagc ttgccagtgt cataaatgcg cggatggcgc
     8341 cttcgggggt atcgtatgtg cttaatccgt gtttgttgaa gatgctgatc ccttcccgca
     8401 tgctttctcc gccgagccag gccgccatga tcggtttcac ggtgtggtcg gccagcttgg
     8461 tgatctccag cgctgtagcg gttggattgg tcatggcttg cggggtgagg ctgaccagga
     8521 cggcgtctac attgggatca tccaggacaa tgcgtgtagc tgtggcaaat cgttccggtg
     8581 gtgcgtcgcc cagcacgtcg accgggttgc cgtgcgacca gaatggcggc aggtaatcgt
     8641 tcagtttctt catggtctca tccgaaagtt tggtcagttt cccatccatg gagatgagcg
     8701 catcggttgc cataacgccc ggaccgccgg cattggttat gatggccagg tttctccctt
//
LOCUS       DAOQXS010000042         8755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8504_length_8755_cov_3.913218, whole genome shotgun sequence.
ACCESSION   DAOQXS010000042 DAOQXS010000000
VERSION     DAOQXS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_8755_cov_3.913218"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..753
                     /locus_tag="PKM34_02235"
     CDS             394..753
                     /locus_tag="PKM34_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82430.1"
                     /translation="MIKPYTLSILPNDGQGFTASVTFDPSHLIFAGHFPGQPVVPGVV
                     LVEIATAFISQLTGKNLVVKEASVIKFLKVVDPRMNWVLLIDCSIVEEGEDGYKANLR
                     IYSGEVDFVKFKGIWFQ"
     gene            789..1190
                     /locus_tag="PKM34_02240"
     CDS             789..1190
                     /locus_tag="PKM34_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNQ82431.1"
                     /translation="MKKLVLLSIVLVGAAIALMSFTFSKVNTSNPPQEQAQAMFPAEI
                     QKIFETSCFDCHSDTGNAKAKLKLNFSKWGELSDAKKVGKIGNINDEVQSGKMPTARY
                     ISNYPDRALTKEQKELVNKWATEESAKLMGQ"
     gene            1187..1657
                     /locus_tag="PKM34_02245"
     CDS             1187..1657
                     /locus_tag="PKM34_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNQ82432.1"
                     /translation="MRKTGWIIIGAVAAAFIIIQFFRPEKNDKVTNPKNDIVFSFNMP
                     VNVKKKIVNACYDCHSETTAYPFYNRVAPVSWIIAGHIREGKKHLNFSDWASYDRKDQ
                     IKLLNEICETITNGEMPLKGYVMMHSKAVFNEREIEEICAWTEQAAEELLSRKD"
     gene            1685..2893
                     /locus_tag="PKM34_02250"
     CDS             1685..2893
                     /locus_tag="PKM34_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNQ82433.1"
                     /translation="MPEFTSYPGFNSQGCCVIIPTYNNEKTLEGVISGVLEFTHDIIV
                     VNDGSTDSTREICAGFDDITVIHLERNRGKGYAIRKGFREALRLGFRYAITIDSDGQH
                     LPEELPKFIEKITAEPDSLIIGARNMDQEGVPGGSSFGNRFSNFWTWVETGYRLPDTQ
                     SGYRLYPVRKMAKMRFITNRFEFEIEVLIRSAWKGIPLTPVPVSVVYHPEKERVSHFR
                     PFTDFARISLLNSVLTILALLFFRPWMMIRKMSKEGFRELVRKHLLSPEESNFRKSTS
                     IALGIFMGIAPVWGWQMMIALALSIALKLNKAITLIASNISIPPMIPFILFGSYITGG
                     LVLGNHQPLDFGPEITLDFVKKNLIQYVIGALVFGAIMGILAGFFTYLLLTVFRRRQK
                     TVAEGTGPGK"
     gene            2918..6823
                     /locus_tag="PKM34_02255"
     CDS             2918..6823
                     /locus_tag="PKM34_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ82434.1"
                     /translation="MSLIFTRIFDFFARYRLVFFLFLGAALAVIIWLLLQLRIVEDIS
                     KVLPHDEKVDQYLEVVNHSAAAEELVFFVGPADASQTIPPEELIRFAGDLADSIRGSL
                     MPGLVAEIRDRSDDLAFKGIYTELHDNLPVFLTEEDYARLDSLTSREAVRNAVKAAYK
                     NLVSPAGMVTRDMVLADPIGLTALALEKRGAPGIDESYRIYDGYVFSSDLRYLIFFIK
                     PAQKPTETSKNTALIKRLDEITGNLSRENNGEIKAGYFGTAAVSVANARQLKRDIILT
                     MNLAIVLLVLFIGFYFGRFRVIPAIVFTTLMSAGISAAVLALIGREISGVSLGFGAVL
                     LGIAIAYFIHYLNHLRDVREPRKVLKEIALPIVMSSIISSGDFFTLLLVRSEAVRDLG
                     LFAGFSILAAGLMTLVFLPHLTKKINWDKSRENLVNRSVRKIASYPVEKKKLVPVMIL
                     LLTVLFYFTSKRVTFEGDLTGMSYMPPELAASESTLDRISQYSLRSVFVVTKGENIDQ
                     ALEQTGRFVQKAERLREEGIVKKYTAVTGLVVPLAEQQRRLERWKAYWTEEKKAEVKG
                     TLTEAATAAGFRAGSFGRFFDWLEKDHKTVGRDDLPELSKIFIDPYISVNDKMATVTT
                     ILKVRQEDKGRLYEALQNETSSFIFDKQYLTSTFMDILNSDFNKLVIISLLIVFVVLL
                     LSYGRIELALITFIPMFMSWIWTLGIMGLFGMKLNIFNMIITSFVFGLGIDYAIFSIQ
                     GMLQQYKYGHGDVTAFRSSVLMDAITTLVGLGVLVLAVHPALKAMAFAAIIGIISVWF
                     VTWSMEPVLFNWLVYQNDRKRPRPVTLKDFIFSISSLSIFIIGSMFIMLYGLLFIKFL
                     RFRTRWAKYALHYQMSAFSKVLIYSNILSSLKINKPEEEDFRKPAMVIANHQSHNDIA
                     LLLILHPKFLEMTNDRVQRNKFFGPLIKMADFYAISDGMESLLPRLRKNVEEGYSVVI
                     FPEGTRSPDGRIQRFHKGAFYLAEELNIDLLPIIIHGSGDIYSKGEYLLKTGRAVIEV
                     LPRITPAEARSKGNLLERSRAIRHYMTAEYRKVAGKAETPRYFRERLVKNYLFKGPVL
                     EWYLKIKVRLEKNYALFHKYLPVDGHITDIGCGYGFMIYMMNFLSDKRTFTGIDYDAE
                     KIEMAKNCPAMNRRIEFIAGDALTMELPKSRAFVMADVLHYFPESEQERLIIRCIENL
                     EDSGLLIIRDGDRKMGKKHIGTRLSEFFSTNLGFNKTRGSAKQLYFTSREKIVEVLMR
                     NGMAVEVIDETKVTSNIVIIARCPHPVPPPAGGGSKGGG"
     gene            6847..8337
                     /locus_tag="PKM34_02260"
     CDS             6847..8337
                     /locus_tag="PKM34_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ82435.1"
                     /translation="MNYDFVITGSGLGGLVSGIILAKEGHSVCILEKNHKPGGNLQTF
                     ERFGHSFNTGLHYIGSMEEGQTLHRFFNYLGIAGKLPLVKMDEDGYDRIVFGEEETVY
                     RHAQGWERFEEEMERQFPSERDSISTYVKSIRDIIRSIPLYDLENASTPRLEMSQLNR
                     CTMDFLRGITRNRRLISVLAGANSIYHGAAGRTPLYLHACIRNSFVSSAWRIKGGSDK
                     LTDLLAETFTSLGGKILTGAEVTGFIPQNDRIASVELRDGRRIEGKQFISNLHPGATL
                     KLTGLETLRKSYRHRIENLENTAGFFSLYLVFRENTFPYLNHNLFQYYHDDYFKDDLP
                     EEKWPHTYMIYTPYHDDNKGFTKTASILTFMKNEQYRQWENLAAAGRGEDYRGFREMA
                     SQKLLDAVEKRYPGIRSSVARFCDATPLTFADYTGTPGGSGYGILKDCHDPVRSIITP
                     KTKIPNLFFTGQNLNIHGVLGTTISAIVTCSEFTGFRNLVNKIVHA"
     gene            8330..>8755
                     /locus_tag="PKM34_02265"
     CDS             8330..>8755
                     /locus_tag="PKM34_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82436.1"
                     /translation="MRRRRAIRVILYILAAFVLIVAGLFAYYKLATKVHEPEIDPSAL
                     ASYERTISGSSVNSCEHGWLKKERDGWWVMYLEGSPYEVGYAHGLLTQELMAAQEKAF
                     LERLAELIPSKFYQRFMLGFVRFFNRNLDQYVKEEYRQEI"
BASE COUNT         2128 a         2277 c         2315 g         2035 t
ORIGIN      
        1 ccttccgggt cgattgcggc ttaaagcccc ggtgagataa aatcaattca aatttcaagg
       61 cggttcaaag gtaaggcatg atatcgaaaa aggcaagatg tttcacagca aaaatcaata
      121 atccgtgtag atccgttgca ttcatgttta tccgtgttct gtcaatagca cgcggatgac
      181 gcgaataata cggattaacg cggatttaaa ttgtctgaaa aaataagttg atacaaattc
      241 agtatcctgg ttgcattctt taatcagatt tagcatgctc ggttgcaatt tttaatcaga
      301 ttcagcatgc tcggttgcaa tttttgattg aattttgcaa gtgcacctta taaacacctt
      361 ttaacaactt tatgaccacc ttataaccac cctatgataa aaccctacac cctatcaatc
      421 ctcccgaacg acggccaggg gttcactgcc tccgtcacct tcgatccctc ccacctcatc
      481 ttcgccggcc acttccccgg gcagccggtg gtgccgggcg tggtactggt cgaaatcgcc
      541 acagctttca tttcgcagct tacagggaag aacctggtcg taaaagaagc atcggtcatc
      601 aagttcctga aagtcgttga tccgaggatg aattgggttc tattgatcga ttgttctatt
      661 gttgaggagg gtgaggacgg gtacaaagct aacctgagga tctattcggg tgaggtcgat
      721 ttcgtaaaat tcaaggggat ttggtttcaa taatgcttat ttttgtcatc atcaaaaaaa
      781 tccatactat gaaaaagctg gtcttattat caatcgtact tgtcggcgcg gctattgcgc
      841 tgatgtcatt cacattttca aaggtaaaca cctccaatcc gccccaggag caggcccagg
      901 ccatgttccc ggctgaaata cagaaaatct tcgagacctc ctgtttcgac tgtcattccg
      961 ataccggcaa tgccaaagcc aagctgaagc tgaacttctc caaatggggc gagctgtcgg
     1021 atgccaaaaa agtgggaaag atcgggaaca tcaacgacga agtacaaagc ggtaaaatgc
     1081 cgactgcgcg ctacatttct aactatcccg accgcgccct caccaaagag cagaaagagc
     1141 tggtgaataa atgggccacg gaagaatcgg ccaaactgat ggggcaatga gaaagaccgg
     1201 gtggatcatc atcggggcgg tcgcagccgc tttcatcatt atccagtttt tccggccgga
     1261 aaagaacgac aaggtaacca atccgaaaaa cgatatcgtt ttctcgttca acatgccggt
     1321 caacgtcaag aagaagatcg tcaatgcctg ctatgattgt cactccgaga cgacggccta
     1381 tccgttttac aaccgggtgg cgcctgtttc atggatcatc gccggccaca tccgggaagg
     1441 gaaaaaacac ctgaacttct ccgactgggc cagctatgac cgcaaagacc agatcaaact
     1501 gctgaatgag atctgtgaaa ccatcaccaa cggcgagatg ccgctgaaag gctacgttat
     1561 gatgcacagc aaggcggtct tcaacgaacg cgagatcgaa gagatctgtg cctggaccga
     1621 acaggcggct gaagaacttt tatcccggaa ggactaaaac ggcattgcac cggatttcct
     1681 gaccatgcct gaatttactt cataccccgg ctttaactct cagggctgct gcgtcattat
     1741 ccctacctac aacaacgaga aaacgctgga aggtgtgatt tcaggcgtac tggagtttac
     1801 ccatgatatt attgtggtaa acgacggctc gaccgattcg acacgggaaa tctgcgccgg
     1861 attcgacgac ataacggtca tccacctgga gcgaaaccgg ggcaagggct atgccatcag
     1921 aaaagggttc cgcgaagcgc tccggcttgg gttccggtat gccatcacca tcgattccga
     1981 cgggcagcac ctcccggaag agctcccgaa atttatcgaa aagatcactg ccgaacccga
     2041 ttccctgatc atcggcgcgc gaaacatgga ccaggaaggg gtgccgggcg ggtcctcgtt
     2101 cggaaacagg ttctccaact tctggacctg ggtggaaaca ggttacaggc tgcccgacac
     2161 gcagtcgggc taccgcctct accctgtccg aaagatggca aagatgcggt tcatcaccaa
     2221 ccggttcgaa ttcgagatcg aagtactgat ccggtcggcc tggaaaggga tcccgctgac
     2281 cccggtgccc gtcagcgtgg tttatcatcc tgaaaaagaa agagtttcac acttccgccc
     2341 cttcaccgat tttgcccgta tcagcctgct caattcggtc ctgactatcc tggccctgct
     2401 gtttttccgc ccctggatga tgatccgcaa gatgagcaaa gaggggttcc gggaactggt
     2461 caggaagcac ctgctcagcc cggaggagtc gaacttccgg aaatcgacct ccatcgccct
     2521 gggcatattc atgggcatag cgccggtgtg gggctggcag atgatgatcg ccctggccct
     2581 ttccatcgcc ctgaaactga acaaagccat caccctgatc gcctccaata tcagcatccc
     2641 gcccatgatc ccgttcatcc tgttcggcag ctacatcacc ggcggactgg tccttggcaa
     2701 ccatcagccg ctcgatttcg gcccggagat caccctggat ttcgtcaaaa aaaatcttat
     2761 ccaatatgtt atcggggcgc tggttttcgg tgcaattatg ggtattttag caggcttttt
     2821 cacttacctc ctgctgactg ttttcaggcg caggcaaaag acggttgctg aagggaccgg
     2881 accggggaag tgatttttta ttaactctta gacgagaatg agcctgatct ttaccaggat
     2941 attcgatttt ttcgcccgct acaggctggt cttcttcctt ttcctggggg cagcgctcgc
     3001 ggtcatcatc tggctcctct tacagctccg gatcgtcgaa gacatctcca aagtgctccc
     3061 ccacgacgaa aaagtggatc aatacctgga agtcgtgaac cattcggccg cggcggaaga
     3121 gctggtgttt ttcgtcggcc cggccgacgc ttcgcagact atcccgccgg aggaactgat
     3181 ccgttttgcc ggcgatctgg ccgacagcat ccgcggttca ttgatgccgg gcctggtagc
     3241 ggaaatccgt gaccgtagcg acgatcttgc ctttaaaggc atttacactg aactgcacga
     3301 taacctgcct gttttcctta cggaagaaga ttatgcccgg ctcgattcac tgacctcgcg
     3361 cgaggcggtg cggaatgctg tgaaggcggc ttacaagaac ctggtctccc ctgccggcat
     3421 ggtcacccgc gacatggtcc tcgccgatcc catcgggctg acagccctgg cgctggaaaa
     3481 aagaggtgca cccggtatcg atgagagcta ccggatctat gacggctacg tattctcttc
     3541 cgatctcagg tatctcatct tcttcataaa accggcccaa aagccgacgg agacctctaa
     3601 aaacaccgcc ctcatcaaaa gactggatga aattaccggt aatttgagcc gggaaaataa
     3661 cggtgagatc aaggcagggt attttggcac cgccgccgtc tctgtcgcca atgcccggca
     3721 gctcaagcgc gatattatcc tgacaatgaa cctggccatc gtcctgctgg tactgttcat
     3781 cggtttctat ttcggccggt tccgggtcat cccggccatc gtcttcacca cccttatgag
     3841 cgcggggata tcggctgccg tcctggccct catcgggcgc gagatctcgg gtgtttcgct
     3901 gggattcggg gcggtgctgc tgggaatcgc catcgcctat ttcatccatt acctgaacca
     3961 tctgcgcgac gtaagggaac cacgtaaagt actgaaagag attgccctgc cgattgtcat
     4021 gagcagcatc atctcctcgg gcgacttctt caccctgctg ctggtccgct ccgaagctgt
     4081 ccgcgacctg ggactgttcg ccggtttcag catcctggcc gcaggattga tgaccctggt
     4141 cttccttccc catcttacaa aaaaaataaa ctgggacaaa agccgcgaaa accttgtcaa
     4201 ccggtcggta cgaaaaatcg cctcctaccc tgtcgaaaag aagaaactgg taccggtgat
     4261 gatcctgctg ctgacggttc tcttttattt cacctcaaag cgggtgactt ttgaaggcga
     4321 ccttaccggg atgagctata tgccgccgga attagccgca tcggaatcga ccctcgacag
     4381 gatcagccag tattcgctgc ggtcggtttt tgttgtgacg aagggagaaa atatcgacca
     4441 ggcgctggag caaaccggcc gctttgtgca gaaggcggaa cggctcaggg aagaaggcat
     4501 cgtcaaaaaa tacactgccg taaccggact ggtggtacca ttggcagagc agcaacggcg
     4561 cctggaacgg tggaaagctt actggacgga agagaaaaag gcagaggtga agggaacgtt
     4621 gaccgaagcg gcgacggcgg caggcttcag ggccggcagc ttcggcagat tcttcgactg
     4681 gctggagaag gaccataaga ctgtaggacg ggatgatctg cctgaactga gtaaaatttt
     4741 cattgatcct tatatttcgg taaacgataa gatggcgacg gttacgacga tcctgaaggt
     4801 cagacaggaa gacaaaggac gactttacga agcgctgcag aatgaaacat cttcgttcat
     4861 cttcgataaa caatacctga ccagcacttt catggatatt cttaactccg acttcaacaa
     4921 gctggttatc atatctttac ttattgtttt tgtggttttg ctcctctcct atggccgcat
     4981 cgagctggcc ctgatcacgt ttatcccgat gttcatgagc tggatctgga cgctgggtat
     5041 tatgggcctt ttcgggatga aactgaacat ctttaacatg atcatcacct cctttgtgtt
     5101 cgggctgggg atcgactacg ccatcttttc gatccagggg atgctgcagc agtacaaata
     5161 cggccacggc gacgtgacgg ccttccggtc gtcggtgctc atggacgcga tcaccaccct
     5221 ggtcggcctg ggggtgctgg tcctggcggt gcatcccgcg ctgaaagcta tggccttcgc
     5281 ggcaatcata gggatcattt cagtctggtt cgtgacctgg agcatggaac cggtgctgtt
     5341 caactggctc gtttaccaga acgaccgcaa acggccccgg ccggtcaccc tgaaagattt
     5401 tattttctct atttcttcgc tgagtatttt cattatcggg agcatgttca tcatgctcta
     5461 cggtttactt ttcatcaaat tcctgaggtt caggacccga tgggcgaagt atgccctgca
     5521 ctaccagatg tcggcctttt caaaggttct catttattca aacatcctat cgtctttaaa
     5581 gatcaacaaa ccggaagagg aggatttccg gaaacccgcc atggttattg caaaccatca
     5641 gtcgcacaac gacatagcac tgctgctgat ccttcatccg aagttcctgg agatgaccaa
     5701 cgatcgcgtt cagcgcaaca agttcttcgg gcccctgatc aaaatggccg atttttatgc
     5761 catttccgac gggatggagt cgcttttgcc caggctcaga aagaacgtgg aagagggcta
     5821 ctcggtggtg atcttcccgg aagggacgcg atcgcccgat ggccgcatcc agcgtttcca
     5881 caaaggagcc ttttaccttg ccgaagagct gaatatcgac cttcttccca ttattattca
     5941 cggatcgggc gatatctatt ccaaagggga atacctcctg aagacgggcc gtgcggtcat
     6001 tgaggtgctg ccgcgtatca cccccgctga agcccgatca aaagggaatc tgctcgaacg
     6061 gtcacgggcg attaggcatt atatgacagc ggaatacagg aaggttgccg ggaaggcgga
     6121 gacgccccgc tatttccgtg aaaggcttgt taaaaattat cttttcaaag ggccggtgct
     6181 ggagtggtac ctgaagataa aggtaagatt ggaaaagaac tatgcactat ttcacaaata
     6241 tcttcctgtg gatgggcaca ttaccgatat tggttgcggg tacggtttca tgatctatat
     6301 gatgaatttc ctttcggata agaggacatt caccgggatc gattatgatg cggagaaaat
     6361 cgaaatggcg aagaactgcc ccgccatgaa ccgccggatc gaattcatcg ccggcgatgc
     6421 cctgacgatg gaactcccga aaagcagggc ttttgtgatg gccgatgtgc tgcattattt
     6481 cccggagagt gaacaggaac ggctaattat ccgttgtatt gagaacctgg aagacagtgg
     6541 cctgctgatc atccgcgacg gcgaccggaa gatggggaaa aagcatatcg ggacgaggct
     6601 ttcggagttt ttcagcacca acctgggctt caacaagacc cggggcagtg caaagcagct
     6661 ttatttcacc agccgggaga agatcgtgga ggtgttgatg cggaatggta tggcggttga
     6721 agttattgat gaaacgaagg tgacgtcgaa tattgtcatt attgctcgct gcccccaccc
     6781 tgtccctccc ccggctgggg gagggagtaa gggagggggc tgaatctgga atctgaactt
     6841 ggaactttga attatgactt cgtcataacg ggtagcggcc tcggcggcct ggtcagcggg
     6901 atcatcctgg cgaaggaggg gcattctgtg tgcatcctgg aaaagaacca taagccggga
     6961 ggcaaccttc aaacgttcga acgtttcggg cattctttca acacgggttt gcattacatc
     7021 ggcagcatgg aagagggcca gacgctgcac cgtttcttca actacctcgg gatagccggc
     7081 aaactgccgc tggtaaagat ggatgaggat ggctacgacc ggatcgtttt cggggaggag
     7141 gagacggttt accgccatgc gcaggggtgg gaacggtttg aggaggagat ggagcggcag
     7201 tttccttcgg aacgagactc catctcaact tatgttaaat cgattcggga tatcatccgc
     7261 agcatcccgt tgtacgacct ggaaaatgcc agtacacccc ggcttgagat gtcgcagctc
     7321 aaccgttgca cgatggactt cctgagaggc atcacccgga accggcggct gatcagcgtc
     7381 ctggctggcg ccaacagtat ttaccacggt gcggcgggcc gtacgccgct ctacctccac
     7441 gcctgcatcc ggaattcgtt cgtgagcagc gcctggcgga tcaaaggcgg gagcgacaag
     7501 ctgaccgacc tgcttgcaga aactttcacc tctcttggcg ggaaaatcct caccggggca
     7561 gaagtcaccg gttttatccc ccagaatgac cggatagcct cggtagaact tagagacgga
     7621 cgccggatcg aagggaagca gttcatctcg aacctccacc ccggagccac tttgaagcta
     7681 acagggttgg aaacccttcg caagagttac cgccaccgga tcgagaacct ggagaacacg
     7741 gccggcttct tcagccttta cctggtcttc agggagaata cctttcccta cctcaatcac
     7801 aatctctttc agtattatca tgatgattat tttaaggatg accttccgga agaaaagtgg
     7861 ccgcacacat atatgatcta tacgccttat catgatgata ataaaggatt tacgaagacg
     7921 gcaagtatcc ttactttcat gaaaaatgaa cagtacaggc aatgggaaaa ccttgctgca
     7981 gcaggccgtg gcgaggatta cagggggttt cgtgagatgg catctcaaaa actgctggat
     8041 gccgttgaaa aaagatatcc gggaatccgt agtagcgtcg caagattttg cgacgctacg
     8101 ccgttgacat ttgctgatta caccggtacg cccggtggca gcggttacgg catcctgaag
     8161 gattgccacg accctgtccg gtcgatcatt acgccgaaaa caaagattcc aaacctcttt
     8221 ttcaccgggc agaacctgaa cattcacggg gtgctgggga cgactatttc ggccatcgtc
     8281 acctgttccg aatttaccgg tttccgcaac ctcgtcaaca agatcgtcca tgcgtagaag
     8341 gagagccata cgggtcattc tttacatcct ggcggcattc gtgctgatcg tcgccggcct
     8401 gttcgcctat tacaagctgg ccacgaaggt ccacgagcct gaaatcgacc catcggcgtt
     8461 ggcctcttac gaaaggacca tatcggggtc ctctgtcaat agctgcgaac acggatggtt
     8521 aaaaaaggag cgtgacggct ggtgggtgat gtaccttgag gggagtccgt acgaagtggg
     8581 ctatgctcac ggattgctga cacaggagct gatggccgcg caggagaaag cgttcctgga
     8641 gcggctggcg gagctgatcc cgtcgaagtt ctaccagcgg ttcatgctgg gattcgtccg
     8701 ctttttcaac cggaacctcg accagtatgt gaaagaggaa taccggcagg agatt
//
LOCUS       DAOQXS010000043         8617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8674_length_8617_cov_3.387293, whole genome shotgun sequence.
ACCESSION   DAOQXS010000043 DAOQXS010000000
VERSION     DAOQXS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8674_length_8617_cov_3.387293"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="PKM34_02270"
     CDS             complement(<1..1818)
                     /locus_tag="PKM34_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82437.1"
                     /translation="MTTSTKKTPGIFPVILMFSLLTAHCSLVYSQVPTVQDCMGAIPV
                     CQDVYVEENSYSGDGNYNNEIYEDLPCNTACPGSCLAGEVNSVWYIWTVQVGGLLRLT
                     IDPVQSTDDYDWAVYDVTDLRCDDIYTSYALMQKSCNAYGAGSNGNTGISTQNGGNSN
                     CNNCGATNIWNKDLPVVEGRTYVLIIENWSGTTQGYTLDFSASTAVIYDDVRPELATV
                     LTDEITCGDTQIVVEFSENVMCESVNPSDFKLSGPGGPYTILGVQGEVCMAGGEMEKR
                     YTLLIDRPINSDGDYSVELKPLNFVYDACNNFAIGNTIVFTVELGAPLINEFNLEINA
                     ATCGLANGSIMGLQIIGTPPYTYLWTDDSGNTVGTTLDLLNVPTGNYTLRVSDNNTCM
                     TPGGPYLVEETGAPLVNDNNILITGANWGANNGHITGLDFSGNEPFVFKWMDDNYDSI
                     GNTPELHDVYSGNYYLLVTDVYACDTLAGPYFVQQIGGPIGVEATASESEICLGEDVQ
                     LFATGFGGAGNYTFSWISNPPGFASDIQNPVVFPAVTTTYTVTISDGYNVTSSSVTVT
                     VHPIPLSNAGQDITINFGTSTTLYGQASGSGGPFAFAWQP"
     gene            complement(1909..2631)
                     /locus_tag="PKM34_02275"
     CDS             complement(1909..2631)
                     /locus_tag="PKM34_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNQ82438.1"
                     /translation="MVTVDIFIPCFIDQIYPQTGFNMIKILEKYDIDVYYNEEQTCCG
                     QISFNSGFWDDAKAIGEKFILEFNHNRPVVVPSASCAGFVRNYYEQLFLNTGLHLEFR
                     QLQKNIVEFTDYLVNTLKVTDLGAVFEHKVTYHDSCAALREYKLGNEPRTLLSHVRGM
                     ELVEMKDTDVCCGFGGTFSVKNEPISTAMAQQKVQNALETGAEYIVSTDASCLIHLDG
                     YIRKHNIPLKTIHIIDVLASGW"
     gene            complement(2702..3955)
                     /locus_tag="PKM34_02280"
     CDS             complement(2702..3955)
                     /locus_tag="PKM34_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ82439.1"
                     /translation="MISISVNSTTLNGTVILPASKSISNRLLILQSRYGNRLKIENLS
                     DSDDTLLLASTLDIVKRYMLTEENGLLRIDARNAGTVLRFLVPLLSITPGHFLLTGIE
                     RMKNRPVGALVDAMRELGASIEYAENTGFPPLLIKGRRIYGHRISVDSSVSSQFVTAL
                     LLLAPTLPDGLTIDLKGTSVSSPYVRMTTGILGKLGIEVISQQDSIRVFPKPELNASF
                     KVEADWSAASFWYCLMSMAESGRVVLPGLKKSNMQGDQSLIDIFRYLGVRTLEENDEI
                     IIERYGTPAEFFAADFTDTPDLALPVIMACAVAGTDGRFTGLESLRLKESDRIEALGA
                     GLAKTGVILFEEYPGAWRIKGRMAARGQLTPEDFSDHRVAMTFACLAMKGYRVHLNNP
                     SVVNKSYPGFWKDLQSVGFNCKVSN"
     gene            complement(3952..5043)
                     /locus_tag="PKM34_02285"
     CDS             complement(3952..5043)
                     /locus_tag="PKM34_02285"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HNQ82440.1"
                     /translation="MARDHNEKIGALNYPVLFGTRSLTNLKSLFLADDNQKTVFVLTD
                     ENTRKYCLPILTDAFCGLEMKQTFTINPGEPSKTMQTAAKIIDWLVDSGAGKEAILVN
                     LGGGVVTDLGGFVASIYKRGIAFINIPTTLMGMADAAIGGKTAVNSGDIKNQIGTFYD
                     PDAVLIFPGFLDTLPASHLKSGYAEIIKCAILEGGDAWDTARNTVSFEDEAFRESFLN
                     AIRFKCRIVSADPYDKSTRQVLNFGHTFGHAFESLFSRSSQPLLHGEAIAAGMICETY
                     LSFVLCGLGRKVLEDLTGFIAGHFRLPALNGDATRDMNRIMAHDKKNRSDEIRFTLLR
                     DAGNPLTGVSAGNSLVNEAFLYYSEIAAK"
     gene            complement(5067..6293)
                     /locus_tag="PKM34_02290"
     CDS             complement(5067..6293)
                     /locus_tag="PKM34_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNQ82441.1"
                     /translation="MDQNQNSFVKFSHAEGLMPQEEMLEIQPQRCRLTIGIPKEISHQ
                     ERRVALVPEAVNLLVQNGHTVLIETNAGAGSRFHDHEFGEAGGQIVFSPQEAYQADII
                     VKVAPPTDEEIETMKDKQVVFSALHLSNREAAFFRNLMRKKITAFAHENIQDPFGSFP
                     VRRSISEIVGNASIMIAARYLSDPEYGKGNMLGGFSGISPTEVVILGAGTVAENAARV
                     AIGMGATVKVFDESIYRLRRLQVNIQNRIFTSVIQPKVLLKALQTADIVIGAIHSING
                     HTPVIVTEEMVMEMKPGAVIIDVSIDQGGCVETSRITTHQNPVFKKYDVIHYCVPNIA
                     SQVPHTASYALSNFFTPVMLRIGEEGGVDHLIRRDRGICKGVYVYKGILTSKPISDLF
                     TIPFQDIDLLLAAFTA"
     gene            complement(6313..6768)
                     /gene="tsaE"
                     /locus_tag="PKM34_02295"
     CDS             complement(6313..6768)
                     /gene="tsaE"
                     /locus_tag="PKM34_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNQ82442.1"
                     /translation="MNILFFDIKDFVIEKFTLQELPAIAARILEQAGQERVFALYGDL
                     GAGKTTLIKALCNKLGVTDAVTSPTFSIINEYVAEGNELVYHFDFYRITRLEEALDIG
                     YEEYLYSGYYCFLEWADKIEELLPEHYVYISIGKSHDEKTRRLTVSLIS"
     gene            6893..8053
                     /locus_tag="PKM34_02300"
     CDS             6893..8053
                     /locus_tag="PKM34_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ82443.1"
                     /translation="MKTGLDAKRIFFNHSGLGNYGRRFYRALATKLTEEEFFLYSPRE
                     VPSGNPYLGEIVGRNSRIVHPSGFFGSKMGGSIWRSAILADQLEKDRIDIYYGLSNEV
                     PFGIHQKRMVRVVIIHDLIFIRYPKLYPATDVFFYRKKTAYACRNCDLIITASEQTKN
                     DIINFYGINARKIHVIYPASDPQFYTGEPPDSSEFFDPRRKYILSIGAITPRKNLMKT
                     VEAFHTVCERLNYDLAVIGTAVGLGRKYLKTIKEYIEKNSLGDRIHFLGNVPYGFVPD
                     ICRKAEMMVYPSQFEGFGMPIVEGLFSRIPVITSGGGCFPEAGGDAAVYIDPENPGEI
                     ADWITRLAGDRELRDELIRKGEKHAERFTQERISADILEFHLSATGHSFRNS"
     gene            complement(8032..>8617)
                     /locus_tag="PKM34_02305"
     CDS             complement(8032..>8617)
                     /locus_tag="PKM34_02305"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HNQ82444.1"
                     /translation="LGWDLRDYEGVEIVSHTGGVDGFVTSVTLIPEQDLGIVVLTNTD
                     ANGFYEALKWEIIDAYMDFPYRDYSRFYLNRYKFFQEQDLDQVNAWRDSVVLKLPLPV
                     EMSRFEGRYVDEVYGYADLKAKGDHLVMTFQHHSDLTGKLEYIGNERFLCTYSHPLWG
                     IKVFPFEIVNGSVKSFTLSVADFLEYTTYEFRKE"
BASE COUNT         2208 a         2146 c         2115 g         2148 t
ORIGIN      
        1 cggttgccag gcaaaggcaa acgggccacc gctgccggaa gcctgaccat acaacgtggt
       61 actggtcccg aaattgatgg ttatatcctg cccggcatta gagagtggaa ttgggtgaac
      121 cgtaacggtt acggaagatg aagtaacatt atagccatcg ctgatggtta ccgtataagt
      181 ggtagtgaca gctgggaaga ccaccggatt ctgtatatcg gaagcaaatc ccggcgggtt
      241 ggatatccat gaaaatgtgt aattccctgc ccccccgaat cctgtagcga agagctgcac
      301 atcttccccc aggcagattt cagattcact ggctgttgct tccaccccga tcggcccgcc
      361 gatctgctgg acaaaatatg ggcctgccag cgtatcacaa gcgtatacat cggtcaccag
      421 cagataataa tttccggaat aaacatcatg caattcgggc gtatttccaa tagaatcgta
      481 attatcatcc atccatttga aaacgaaagg ttcgttgccg ctgaagtcca gtccggtaat
      541 atgtccgtta ttggcgcccc agttagctcc ggtaatcagg atgttattgt cattcaccag
      601 cggtgcgccg gtttcctcaa cgagatacgg tccgcctggc gtcatacagg tattattgtc
      661 ggaaacacga agggtataat ttcctgtcgg tacgttgagc agatcaagcg tagttccgac
      721 cgtgttgccc gaatcatcgg tccagagata ggtatacggt ggtgtcccga tgatttgaag
      781 gcccataatt gatccattgg ccaggccaca ggtagccgcg ttgatttcca aattgaattc
      841 gttgatcagc ggggctccca gttccacagt aaaaacgatc gtgttgccaa tggcaaagtt
      901 attgcaggcg tcgtacacga aattcagggg tttcagttcc accgagtaat ctccgtcgga
      961 attgatcggg cggtcgatca gcagcgtata gcgtttctcc atttcaccgc cggccataca
     1021 aacttcaccc tgcactccga gaatggtata tgggccgccc ggaccggaca gtttgaagtc
     1081 ggagggattc accgactcac acatgacatt ttcggaaaat tcgaccacga tctgggtatc
     1141 gccacaggtt atctcatcgg taaggacagt ggccagttcc ggtctgacat catcataaat
     1201 aaccgcagta gacgcgctga agtcaagagt atagccctgg gtggtgccgc tccagttttc
     1261 gatgatcaac acataggtcc gtccttcgac gacgggaagg tctttgttcc agatattagt
     1321 agctccgcaa ttattgcaat tgctgtttcc tccgttttgc gtgctgatcc cggtgttgcc
     1381 atttgaaccg gcgccataag cgttgcagct tttttgcatc agggcgtatg aagtataaat
     1441 atcgtcgcaa cgcaggtcgg ttacatcata aacagcccag tcataatcat cggtactctg
     1501 aacagggtcg atcgtgagcc tcaaaaggcc tccgacctgg acggtccaga tataccagac
     1561 actgttcact tccccggcaa ggcaactgcc cgggcaagcg gtattgcacg gcaggtcttc
     1621 ataaatttca ttgttataat tcccgtcgcc gctgtatgaa ttttcttcga catatacatc
     1681 ctggcaaacc gggatggcac ccatacagtc ctgaaccgta ggaacctgag aatagacaag
     1741 tgaacagtga gcggtgagca gtgaaaacat taaaataaca ggaaatatgc ctggcgtttt
     1801 tttcgttgaa gtagtcataa gcaataggtt aatgaagctg atgccactcc aaaaatagtt
     1861 aaaatcgaat aaaaacggat ataaaatatg aatactgcgg gtgatttttt accatcctga
     1921 agccagaaca tcaataatat gaatggtttt caaaggaata ttatgtttac ggatataccc
     1981 gtcgagatgg atcaggcagg aagcatcagt cgacacaata tactccgcac cggtctccag
     2041 ggcgttctgg actttttgct gggccatggc cgttgatatg ggttcgtttt tgaccgaaaa
     2101 agtgccccca aatccgcagc acacatctgt atctttcatt tcgaccagtt ccatgcctct
     2161 gacatggctg aggagggttc ggggctcatt tcccagttta tattcccgaa gagcggcgca
     2221 ggagtcatga taggtgactt tatgctcaaa tacggcgccc aggtcggtaa ctttaagagt
     2281 attgaccaga taatcggtaa attcgacgat atttttctgc aactgcctga attcgagatg
     2341 taaacctgta ttcaggaaca attgttcgta ataatttctc acaaaacctg cacaggaagc
     2401 agacggaaca acgaccggcc tgttgtggtt gaactccagg ataaactttt cgccgatggc
     2461 ttttgcgtca tcccagaatc cgctgttgaa agagatctgg ccgcagcagg tttgttcttc
     2521 attatagtaa acatcgatat cgtatttctc cagtattttg atcatattga agcccgtttg
     2581 gggatagatc tgatcgatga agcacgggat gaaaatatcg acagttacca taattctgat
     2641 gaaaaaatga cgaatcaggg gcaaaggtat caaattatct taaggcggat tcaattcctc
     2701 atcaatttga aactttgcag ttaaaaccta cagattgaag gtctttccag aatccgggat
     2761 aggatttatt cacgaccgac gggttgttca aatgaacccg gtaacctttc atggccaggc
     2821 aggcgaaagt catggccacc cggtgatcgc tgaaatcttc aggggtcagc tggcccctgg
     2881 ctgccattct tcctttaatc cgccaggcgc ccgggtattc ttcaaaaaga atgaccccgg
     2941 ttttagccag cccggctccc agcgcttcga tccggtctga ttctttcagc cggaggctct
     3001 ccagcccggt aaaacggcca tcggttccgg ccaccgcaca agccatgatc accggcaaag
     3061 cgagatcggg cgtatcggta aaatcggcag caaagaattc cgccggagtt ccgtatctct
     3121 ctatgattat ttcatcattc tcctccaaag tccttacccc cagatacctg aaaatatcga
     3181 tcagcgactg gtccccctgc atatttgatt ttttgagtcc cggtaaaact acccgtccgc
     3241 tttcggccat cgacatcagg caataccaga acgatgctgc gctccagtcg gcttcaacct
     3301 taaatgaagc gttcagttcc ggtttcggaa aaacacggat ggaatcctgc tggctgatta
     3361 cctcgatgcc caatttgcca agaatcccgg tcgtcatccg aacataaggc gatgataccg
     3421 atgtcccctt caggtcgata gtcagtccat ctggaagtgt tggggccagt aacaaaagcg
     3481 ctgtaacaaa ctggctgctt accgaactgt caaccgaaat ccggtgcccg taaatacgcc
     3541 tgcccttgat cagcagcggc ggaaatccgg tattctcagc gtattcaatg gaggctccca
     3601 gttcccgcat ggcatctacc aatgcaccaa ccggacgatt tttcatcctt tcgatgccgg
     3661 tgagcagaaa atggccgggt gttatcgaga gcaacggaac aagaaaacga agaacggtcc
     3721 cggcattccg ggcatcgatt ctcagcaggc cgttctcctc cgtcaacata taccttttga
     3781 cgatatccag cgtggatgca agcaacaggg tatcatccga atccgacaga ttctcgattt
     3841 taagccggtt accatagcgc gactgaagga tcagcaaccg attcgaaatg cttttcgagg
     3901 ccggtaagat gaccgtacca ttcagtgtcg tgctatttac agagatggat atcatttcgc
     3961 cgctatttca ctataatata aaaatgcctc gtttacaagg ctatttccgg cactgacgcc
     4021 tgttagaggg ttccccgcat cacggagcag tgtaaaccgg atttcatccg aacgattctt
     4081 cttgtcgtgc gccatgatcc ggttcatatc acgggtggca tccccgttaa gcgcgggcag
     4141 tctgaaatgc ccggcgataa aaccggtcaa atcttcaagg acttttcggc cgagcccgca
     4201 gagcacaaaa gaaagatagg tttcgcagat catgcccgca gcaatcgctt caccgtgtaa
     4261 aagtggctgt gaagagcggg aaaacaggct ctcaaaggcg tggccgaaag tatggccgaa
     4321 attcaatacc tgccgggttg atttgtcata aggatcagca cttacgatcc ggcacttaaa
     4381 gcggatcgca ttcaggaagc tttccctgaa agcctcatct tcgaaagaga cggtattacg
     4441 tgcggtatcc cacgcatcac caccttccag gatggcgcat ttgataattt cagcgtagcc
     4501 cgacttcagg tgggatgccg ggagggtatc gagaaatccc gggaagatga gcaccgcatc
     4561 cggatcataa aaggttccga tctggttttt gatatctcct gagttgactg ccgttttacc
     4621 gccgatggcg gcatcggcca tacccatcag ggtagttggt atgttgatga atgcgatgcc
     4681 tcttttgtaa atggaagcga cgaacccgcc gaggtcggtg accactcccc cgccaagatt
     4741 gaccagaatt gcctcttttc ctgctcctga atcaaccagc caatcgatga tctttgcggc
     4801 cgtctgcatg gttttcgacg gctcgccggg gttgatcgta aatgtctgct tcatttccaa
     4861 accgcaaaag gcatcagtta aaattgggag gcaatactta cgggtatttt catcggtaag
     4921 gacaaatacg gtcttttgat tatcatcagc taagaataac gatttcaggt tcgtcaacga
     4981 tcgcgtgccg aacagtaccg ggtagtttag tgctccgatt ttctcgttat gatcccgtgc
     5041 cacctgacct ggaaggaatt aatcggttaa gctgtaaatg ccgccaggag taagtcgata
     5101 tcctggaaag gaatggtaaa cagatcgctg ataggtttac tggtaaggat acctttatac
     5161 acgtaaactc ctttgcagat accccggtcg cgacggataa gatgatcgac tccaccctcc
     5221 tccccgatgc ggagcatgac cggtgtaaag aaattgctca gggcgtagga ggcggtatga
     5281 ggcacctggg aagcgatatt cgggacacag taatgaatga cgtcgtattt tttgaacacg
     5341 gggttctgat gggttgtgat ccgggaagtt tccacgcatc cgccctggtc gatactgaca
     5401 tcgatgatga ccgcgccggg tttcatttcc atgaccattt cctcggtgac aataacgggt
     5461 gtatggccgt ttatactatg gatggccccg ataacgatat cggcagtctg aagggctttt
     5521 aagagcactt tgggttgaat gacggaggta aagatacggt tctgtatatt cacctgcaga
     5581 cgccgtagcc tgtatatcga ttcatcgaat accttaacgg tcgctcccat gccgatggct
     5641 acccgggctg cgttttccgc aaccgtgccg gcgccgagga tcacgacttc cgtcgggctg
     5701 atccccgaaa aaccgcccag catgttgcct tttccatatt ccggatcgct caggtaccgg
     5761 gctgcgatca tgatggaagc attgccgact atttcactga tagaccgccg gacgggaaaa
     5821 gagccgaacg ggtcctggat gttttcgtgg gcaaaggctg taatcttttt ccgcatgagg
     5881 ttcctgaaga aggctgcttc gcggttactc aggtgtaaag ctgaaaagac cacctgcttg
     5941 tctttcattg tttctatctc ctcatctgtc ggcggagcga ccttaacgat gatatccgcc
     6001 tggtaagctt cctgcgggga gaaaacgatc tgtccgcctg cttcgccaaa ttcatggtca
     6061 tggaaacgtg agccggcgcc ggcgtttgtt tcgatcagca cggtatgtcc attctgcacc
     6121 agcaggttca cggcctccgg caccagcgcc acccgccgtt cctggtgcga aatctccttc
     6181 gggataccga tggtcagccg gcagcgttgc ggttgtatct caagcatctc ctcctggggc
     6241 atcaggccct ccgcatgcga aaacttaaca aagctattct ggttctgatc catcgcaggg
     6301 aacattaatg cgttagctaa tcagggaaac cgtcaaacga cgggtttttt catcatggct
     6361 tttcccgatg gaaatgtata cgtaatgctc cggcaaaagt tcttcaattt tatccgccca
     6421 ctccaggaaa caataatatc cgctgtaaag gtactcctca taaccaatat ccaacgcttc
     6481 ttccagcctc gttatccggt agaaatcaaa atggtacacc agctcattac cttctgcaac
     6541 atactcattt ataatggaga aggttgggct ggtcaccgca tcggtaacgc ccagtttatt
     6601 acataaagct tttatcaggg tggtctttcc cgcgcccagg tcgccataca aggcaaatac
     6661 cctttcctgg ccggcctgct caaggattct cgcggcaatc gccggcaatt cctgaagggt
     6721 gaacttttca atcacaaaat ctttaatatc gaaaaagaga atattcaaag atacgatatt
     6781 tttaccggaa ttttttgcac tttcgtggtg gatttttacg ttagataaaa ataatgaagc
     6841 gggtcgtaaa agtgtaatat aatcactgca aggcgcttat aagataaatc agatgaaaac
     6901 gggcctcgat gccaagcgga tctttttcaa ccatagcggg ctgggtaact acgggcgcag
     6961 gttttaccgt gcgttggcca cgaaacttac ggaagaagaa tttttcctgt attcgccccg
     7021 ggaggtacca tccggtaatc cgtacctcgg ggagatcgtc ggccgtaaca gccggatcgt
     7081 gcatccatcg gggtttttcg gcagcaagat gggaggaagc atctggcgtt cagctattct
     7141 tgcggaccaa cttgaaaaag accggatcga tatctactac gggttgagca atgaagttcc
     7201 attcgggata catcaaaaaa gaatggtccg tgtggtcatc atccacgacc tgatctttat
     7261 ccggtacccg aagctttatc cggccacgga tgtgttcttt tacaggaaga agacagcgta
     7321 cgcctgccgg aattgcgatc ttatcataac ggccagcgag cagaccaaaa acgatataat
     7381 caatttttac ggtatcaatg cccgtaagat ccatgttatt tatcccgcaa gcgatccgca
     7441 attttacacc ggggaaccgc cggactcatc ggaatttttt gatccccggc gaaaatatat
     7501 cctgagtatc ggggccatta ctccccggaa aaacctgatg aagacagtgg aggcattcca
     7561 cactgtgtgc gaacggctga attacgacct ggcggtgatc ggaacggctg tcggattagg
     7621 gcgtaaatac ctgaaaacga tcaaagaata tattgaaaaa aacagcctgg gcgaccgcat
     7681 ccattttctg ggaaatgtgc cttacggatt cgttcccgat atctgccgga aggctgaaat
     7741 gatggtttat ccatcacagt ttgaaggatt tggaatgccg atcgtggaag gtttgttcag
     7801 ccggataccg gtcattacgt ccggaggcgg atgttttcct gaggccgggg gcgatgctgc
     7861 cgtttatatt gatcccgaga atcccggtga aatagcggat tggatcaccc ggcttgccgg
     7921 cgaccgtgaa ctgagggatg aattgatcag aaaaggcgaa aagcatgcgg agcggtttac
     7981 tcaggagagg atcagtgcag atatcctgga atttcacctt tcagccaccg gtcattcttt
     8041 ccggaattca taagttgtat actcgagaaa atcggctacg ctgagggtaa acgatttaac
     8101 gctgccgttc acgatctcaa acggaaatac ttttatcccc cagagcggat gactgtatgt
     8161 acagagaaaa cgttcgttcc cgatatattc cagttttccc gtaaggtcgg aatgatgctg
     8221 aaaagtcatg accaggtgat ccccctttgc cttgaggtcg gcatagccat aaacctcatc
     8281 aacatatcgt ccttcaaaac ggctcatttc aaccggaaga ggcagtttca gcacaaccga
     8341 atcgcgccag gcattcacct gatccaggtc ctgttcctgg aagaatttgt aacggttcag
     8401 atagaaccgg ctataatcgc ggtaaggaaa atccatgtag gcatcgatga tctcccattt
     8461 gagcgcttcg taaaagccgt tggcatcggt attggtcagc acgacgatcc ccaggtcctg
     8521 ctccgggatc agggtaacag aagtgacaaa tccatcgaca ccacccgtat gcgacacgat
     8581 ctcaacgcct tcataatcgc gcagatccca tcccagg
//
LOCUS       DAOQXS010000044         8597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8692_length_8597_cov_3.427769, whole genome shotgun sequence.
ACCESSION   DAOQXS010000044 DAOQXS010000000
VERSION     DAOQXS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8692_length_8597_cov_3.427769"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..6323
                     /locus_tag="PKM34_02310"
     CDS             <1..6323
                     /locus_tag="PKM34_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82445.1"
                     /translation="GRFLYVYSNSALESLTVKGNTLDNLVLKKSAIRIENNQYLNALT
                     LESNDYSNFITDVTYPVADGIIYIKGQNILNQFRLDDNTIQTILSSQQLNPPVSSFLY
                     LDHAANAYFKLDNVVSVSSLNDFILVEDCETVRVEDSKFESLECSGALFNLSADKYCR
                     VEDGIFKDISTNDGNGCFYFSQVASMDEILFRNNRFEAVTAENGPCLYVNSPSGSVDS
                     VVFRNNDIFQDRMGYTAINGGVAYLRLSSVSLFRSYDNEIRNCEVTGYGGAYCFDVGT
                     VNNLDIYNDDLHTIISNYDGGYISVKNQITNTDISINGLTADTCFSKAGSGGILYLKS
                     QNNNTGKVRIQNNHLNHFYARSESQQFAGGGFFIRGSHKELHFTGNALIPNPTPDQIP
                     IADDGGVLYYAHTGSVLDSAIFSGNSFIKADSVAITGSGGAVYLTSTGQTGRIRISDN
                     EFDRFSTAGSGGVFWIGFEGQCNRLDILNNEFSGCVADSSGGAIFFRSSGLNVLNVAG
                     QPGENQLFTDCRARFASGGSLYVLTQAQSIGEINFNHNSFNSPGSAQNALSDGGAVYV
                     KSAGSLVSHLNLSENSVNENNMDIRAGGNGGAFYFNLMGNCTEVNLLNNSLSSCLAGR
                     SGGAVYLRTGNIGRLTVEGETASPQVKDCHAANGGAYYFDLSGKCDTLDITSNNFMNC
                     AADTTGGAIYFKSNGIGLLNISAQDGDQQKFNNCQAASGYGGVMYFNSLSQGIGEIGI
                     RNNQILSSLNENNSGKDGGGFYFKSVGSDVANIDIRNNTVGNSNKGLKAGMNGGAFYF
                     DLSGKCNQLSIHQNNFNNCRAVNGSGGSIFFLSGGAGTVSIDTNMLLSSSAIENASAN
                     GGGIYIKTNGGNVTQISLNGNEAGDDSFGISSGENGGAFSFDLQGTTTLLAVTGNRFV
                     KCRAQDSGGGLYFKANGLNELKLQGFEDELQHFSGCSAADGSGGAVCITTTSQPVSYA
                     YIEYNTVLPAAGMVNAGENGGGISITSAGNLVEECFVDHNIFGSASGSLKAGNSGGAI
                     SVDISGQCNTLRLFENSFTGCQAVNNGGAVSIHAGSLGTLTVSGSPDQYQVFGDCSTE
                     NLGGCFYLESDGVGHADFQHLKFENSTAESGGALAFSSPDMDTLSLNTIEFTDNSASD
                     FGGSIYLKGDGNNMPTIDVLEMNKCTFINSEADNGDGGALFFKGNLGLFNCSSNSFNQ
                     CEANGANSGSGSAMYLELSDDFTNELVFTSDTVKNCHSVSGGIFLKNIDKATFLEGHL
                     VGNTATQDGAAIYIQDFDTVLITGGLFSQNESQQTGGAILLKAGNSESSCCDIGSSEF
                     EFNKALNGGAVTLLDFNQSSLLNNRFIQNSAFSQSMECSGGAVYTVDIDDLVFDGNEF
                     ISNSASTTSTQNNIQSYGGSLCILNNKYFEGSGNDFRFSNAEVGGAVFAKNTDATLNP
                     SVKYDNNFFYNNGSTFGGAVYYDLMNKGGIYATENVFLMNGAGFSGGAIDLRNIGRFE
                     SVRNIFRSNFNEGKNLSGVVGASAILTWNVPESNYYNTVFDYNFYEKDTITERESTIW
                     IKNHDNVYFENCTFLNQIPQDNPVIYKDQNSEPLTIVNSIFSKNVTPETPLVNDFAEI
                     RHSYIEGLITEKIDTMNCFRYPPPQFVDGTYNLNDNDFAFIDAGIPEAVYNDSIDYPI
                     GKGTETCDLGVSGGRYNLWDEEQLSIPLANQYSRTIIIERYNGECGRYRIYFALNEGD
                     EFTDYTWYIFDEIHKTGSVNEFIGYFPQDQLIAVTGVASNESEGITVIGNNTFSTFYE
                     ITLDGSQISYEGSDYQCGATIELNDNCPVQLNNIVLHLEGLDMPFFSSYKFGWSFRDA
                     IGIASQNITDQSYEEVVFSLDMEADTTSLRSVTVVYNGYDTVCDITFEYECLIRFHYS
                     NLENVLELVEIAPPDMILDPGNVMFDVKLNDPAYYCNGVDFYLIEEGSVATSLDFIRL
                     RSVATGNTVPIQGITYAGNIFQLTFNVTDLPKGEYDVEMQNLCNSCGFPFTLVNRIDI
                     KFEGIAGMADSELKIWPVPANEKIFIENEKTASVMRFSLVDPLGEILFTDNIYPGKRY
                     ELNTGRYPGGIYILIFEPFDSKIKSYRKIIIE"
     gene            6328..8085
                     /locus_tag="PKM34_02315"
     CDS             6328..8085
                     /locus_tag="PKM34_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82446.1"
                     /translation="MKKKSLLLIFSLFTLFGFCQQEVFWFPAKGRVYGDVMRIMDDNN
                     PYVRNRIQGFNEGDINVNPEYWFNGFKAPANDFEWSNQSGVYYSQLDYPSILADKDVT
                     TIILDFVTVWDEQFYDTAEYYYSKPVMECLTVNNGSYSENMLLVNPTEVTLDFRLYAS
                     LFNLPAPTDGDDRIDGIVEVIDGNVKPINIADTRNGTYIHGVDLSSLSYGLHELEYYA
                     YGQKSDVNLPDEFSDKIKIRILIFDFKLRYDEDYAVCKCDTAYDLEGRPEGGIFSGEC
                     VVGSSNVFNPELTQNASTLITYKYPVDGMYYNVERTVSFDPLPVIAVDATTLAGFPHE
                     ACGFEHGAVYEVTGTGFEDLFRLPPLGIIKQFHYDGNKLTIDWKGEGDGSIYVTALSD
                     KGCSASLEHMVHITFNRAPEDSAYVTLYDRMLFCNADTASVKVFDWYGVIGGSNVFYE
                     RTTNPYYVLDFIPAAGDSFYVLTADDTTSCSTHSHIYTKPAVPKSDLIGEGAEQPAVK
                     LFPNPVRGDVNCEILKPVKEGRIIITDVLGRQISRINHDGFLPGEVVRLETGDFKSGI
                     YYVVFSGNNLSGSYKFIVY"
     gene            8101..>8597
                     /locus_tag="PKM34_02320"
     CDS             8101..>8597
                     /locus_tag="PKM34_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82447.1"
                     /translation="MMDNRVKYTLFILLTFAALNGFSQKIPAKLEAEVSDQVSETIAK
                     FTRQISSIYTSAGETSFRFDSVALNRTRTMFYKYNDPSFKHISPNIIDPSKEFGLSSF
                     PKDTNILPENFIVPIDQFLDSLAAWYCNDSCITTLELGYMDPKTDWNNYYIDPQLLCL
                     QVFYK"
BASE COUNT         2339 a         1899 c         2039 g         2320 t
ORIGIN      
        1 ccggccggtt tctttatgtc tattcaaatt ctgcccttga atcactaact gttaaaggta
       61 ataccctgga taacctggtt ttaaagaaat cagccatcag aattgaaaat aaccagtatc
      121 tgaatgcact gaccctcgaa tcaaacgatt acagcaattt tataacagac gtcacctacc
      181 cggtcgctga tgggataatt tacattaaag gccagaatat tctcaaccag ttccgcctgg
      241 atgataatac catccaaaca atcctgtctt ctcagcaatt aaatccgccg gtaagttcct
      301 tcctttatct tgatcatgcc gccaacgctt attttaaatt ggacaatgtg gtttccgttt
      361 ccagtttgaa tgattttatc ctggtggaag attgcgaaac tgtaagagta gaagattcaa
      421 aattcgaatc attagagtgt tccggcgccc tttttaacct ttctgccgac aagtattgcc
      481 gtgtggaaga tggtattttc aaagacattt caaccaatga cggaaatggc tgtttctatt
      541 tcagccaggt tgcatcaatg gatgaaatcc ttttcagaaa taaccgtttt gaagctgtca
      601 cagccgaaaa tggcccgtgc ctttatgtca attcgccctc cgggtcggtc gattccgttg
      661 ttttcaggaa caatgacatc ttccaggacc ggatgggata tacggccatc aatggcggag
      721 tggcttatct ccggttgtca tccgtttcat tgttcaggag ctatgataat gaaatccgga
      781 attgtgaagt caccggatac ggcggtgcct attgttttga tgttggcaca gtcaataatc
      841 ttgatattta taatgatgat ctccatacga ttatctcgaa ttatgacggc ggttacattt
      901 cggttaagaa tcagataacc aataccgaca taagcattaa cggcctgact gccgatacct
      961 gtttttcaaa agcaggatca ggcggtatcc tttatctgaa gtctcaaaac aataataccg
     1021 gaaaagtcag gatacaaaac aaccatctca atcatttcta tgcccgcagt gaatcgcagc
     1081 aatttgccgg gggtggtttt tttatccggg gcagccacaa ggagctgcat tttaccggta
     1141 acgcccttat tcccaatccg acaccggatc agatcccaat tgcagatgat ggcggagtgc
     1201 tttactatgc ccacactggc agtgttctcg attcggccat tttttccggt aattccttta
     1261 ttaaagccga ttctgtagcc attacaggtt ccggtggagc cgtttacctg acaagtacgg
     1321 gccagaccgg ccggataagg atcagtgaca acgagttcga ccggttcagc actgccgggt
     1381 caggaggtgt tttctggatc ggctttgagg gccagtgtaa ccggctggat attctcaata
     1441 atgaattctc gggttgcgtt gccgatagtt ccggcggcgc catctttttc cggtcatccg
     1501 gattaaatgt cctaaacgtg gcaggccagc ccggtgaaaa ccagcttttt accgactgca
     1561 gggcccgttt tgcatccggc ggaagccttt acgttttaac ccaggcccaa tccatcgggg
     1621 agatcaactt taaccataat tcattcaatt cccccggctc cgctcaaaat gccctgtcag
     1681 acggcggtgc tgtttatgtg aaatcggccg gttcactcgt gtctcattta aacctgtcgg
     1741 aaaactctgt caatgaaaat aatatggata tccgggccgg tggaaacgga ggagcattct
     1801 atttcaatct gatgggtaac tgtactgagg tcaacctgtt gaataattcc ctttcttcct
     1861 gccttgcagg ccgttccggc ggggcagttt atctcaggac cgggaatatc ggcagattga
     1921 cagtggaggg cgaaacagcc agcccacagg tgaaagactg tcacgccgca aacggtgggg
     1981 cttattattt cgatctgtca ggcaaatgcg ataccctcga catcacttct aacaatttca
     2041 tgaactgtgc agccgacacg accggaggcg ctatttactt taaatcaaac gggatcggtt
     2101 tgttgaatat atccgcccag gacggagatc agcaaaaatt caataactgc caggctgcca
     2161 gcggatatgg cggtgtgatg tattttaaca gcctttcgca gggcatcggg gaaatcggca
     2221 ttcgtaataa ccagatcctg tcatcgctta acgaaaataa cagcggaaaa gacggaggcg
     2281 gtttttattt caagtcggtg ggcagtgatg tggcaaatat cgatatccgg aacaacaccg
     2341 ttggaaacag caacaaagga ttgaaagcag gcatgaacgg aggcgcattt tattttgacc
     2401 tctccgggaa gtgcaaccag ctcagcattc atcagaataa tttcaacaat tgtcgcgctg
     2461 tgaacggatc gggtggcagc atctttttcc tctccggcgg ggcaggaact gtttcgattg
     2521 acaccaacat gctgctatca tcatccgcaa tcgagaacgc ttcagccaat ggcggcggaa
     2581 tttacattaa aaccaacggc ggaaatgtga cacaaatctc actgaatggt aatgaagcgg
     2641 gtgacgacag ttttggcatc agctccggtg agaatggagg agcttttagc tttgatctgc
     2701 agggaactac gacattgctg gcagtgaccg gaaaccggtt tgtcaaatgc cgggcgcagg
     2761 actcgggcgg cggcctctat tttaaagcca acgggctgaa tgaattaaaa ctacagggat
     2821 tcgaagatga attgcagcat ttcagcggtt gttcggctgc cgatggctcc ggaggtgccg
     2881 tttgtatcac aactacatcg caaccggtta gttatgccta tatcgaatac aatacggttc
     2941 ttccggcagc cggcatggtg aatgccggtg aaaacggcgg aggaatcagc attacatcag
     3001 ccgggaacct tgtcgaagaa tgttttgttg accacaacat attcggaagt gcttcaggta
     3061 gtctgaaagc cggaaatagc ggcggggcaa tatcagttga tatttccggt caatgcaata
     3121 ctctcagatt atttgaaaat tcattcaccg gctgccaggc tgtaaataat ggcggtgcag
     3181 tgagcatcca tgccgggagc ctcgggaccc tcactgtttc aggctccccg gatcagtacc
     3241 aggtattcgg tgattgttcg acagaaaacc ttggtggctg cttctaccta gagtccgacg
     3301 gtgtaggtca tgcagatttc caacacctta aatttgagaa ctcaaccgca gaaagcggag
     3361 gggccttggc tttttcatcc cctgatatgg atacgctgag cctcaacacc atcgaattta
     3421 ctgacaactc agcctcagac ttcggcggat cgatatattt aaaaggagac gggaacaaca
     3481 tgcccaccat tgatgtattg gaaatgaata aatgcacctt tatcaattcg gaagcggata
     3541 acggggatgg cggcgctttg tttttcaaag gtaacctggg cctttttaat tgttcatcca
     3601 acagttttaa ccaatgtgag gccaatgggg ctaacagtgg ttcaggttct gccatgtacc
     3661 tcgaactatc tgacgatttc acgaatgaat tggtctttac atccgatacg gtcaaaaatt
     3721 gccattctgt ttccggagga attttcctta aaaatatcga caaagccacc ttcctggaag
     3781 gccatctcgt gggaaatacc gccacccagg acggagcagc catctatatc caggatttcg
     3841 ataccgttct gattaccgga gggctcttca gccagaatga aagccagcag accggcgggg
     3901 ctatcctgct gaaggccggg aattcggaaa gttcctgctg tgatataggt tcctcggaat
     3961 ttgaattcaa taaagccttg aacgggggtg cagttacctt gcttgatttt aaccaaagtt
     4021 cattgctgaa caaccgtttc atccagaatt ccgctttcag tcaatccatg gaatgctctg
     4081 gaggagctgt ttatacagtt gatattgatg atcttgtttt cgatggaaat gagtttataa
     4141 gcaattcagc cagcaccact tccacgcaga acaatattca atcatacgga ggcagtctgt
     4201 gtatcctgaa caataagtat tttgagggat ccggaaatga tttccggttt tcaaatgcag
     4261 aggtgggtgg agctgtcttc gcaaagaata ctgatgctac actgaacccg tcggtgaagt
     4321 atgacaataa tttcttctat aataatggaa gtacttttgg cggggccgtt tattacgacc
     4381 ttatgaacaa aggaggcatc tatgcaactg aaaacgtatt cctgatgaat ggcgcgggat
     4441 tcagcggcgg tgcgatcgat ctgagaaaca ttgggcggtt cgaatccgtg cggaatattt
     4501 tcagaagcaa tttcaatgag gggaaaaacc tcagcggcgt cgtgggcgct tcggccatct
     4561 taacctggaa cgtgccggaa tccaactatt acaatacggt cttcgattat aatttttacg
     4621 aaaaagacac gataaccgaa agggaatcaa ccatctggat taaaaaccac gataatgtct
     4681 attttgagaa ttgtacattc ctgaatcaaa taccccagga taatccggtt atttacaagg
     4741 atcagaacag tgaaccgctg accattgtca attccatctt ttcaaagaat gtaactcccg
     4801 aaacaccttt ggtaaatgac tttgccgaga tccggcactc ctatatcgaa ggcctgatta
     4861 cagaaaagat agatacgatg aattgcttcc gctacccgcc gcctcagttc gtcgatggca
     4921 cttataatct taatgataac gacttcgctt ttatcgatgc cggaattcct gaggctgtgt
     4981 ataacgattc gattgattac cccatcggca aaggaaccga aacctgcgac ctgggtgttt
     5041 ccggcggccg ctataacctc tgggatgagg aacaattgtc tattcccctg gcaaaccagt
     5101 attcccgtac catcattatc gaaagatata acggtgaatg tggccggtac agaatttatt
     5161 ttgctctgaa tgaaggagat gagtttacgg attatacatg gtatattttc gatgagatcc
     5221 ataaaaccgg atctgtaaat gagtttatcg gttattttcc tcaggatcaa ctcattgctg
     5281 taaccggggt ggcatcaaat gaaagcgagg gcataacggt tatcggtaat aatacgttca
     5341 gtacattcta tgaaataacg ctggatgggt cacagatttc ctacgaaggg agcgactacc
     5401 agtgcggagc gaccatcgaa ctgaatgaca attgtccggt gcaactgaac aatattgtat
     5461 tgcacctgga gggtctggat atgcctttct tcagttctta taagtttgga tggtcattcc
     5521 gggatgccat cggaattgcg agccagaaca taaccgacca gagctatgag gaggttgtat
     5581 tcagcctgga tatggaagcg gacacaactt ctctgcgttc cgttaccgtt gtgtacaatg
     5641 gctatgatac cgtatgcgat atcacattcg aatatgaatg cctgatccgg ttccattaca
     5701 gtaacctcga aaatgtgctg gaactggtgg agattgcacc gcctgatatg atccttgatc
     5761 ctggtaatgt gatgtttgac gtaaaattga acgacccggc ttattactgc aacggagttg
     5821 atttttacct aatagaagaa ggcagtgttg caaccagcct ggatttcata aggctgagat
     5881 cggtcgcaac cggaaacacg gtgccgatcc aggggataac atacgccggc aatattttcc
     5941 agttgacatt caatgtaaca gaccttccca aaggggaata cgatgtggaa atgcagaatc
     6001 tgtgcaattc ctgcggtttt ccgtttactt tagtcaaccg gattgatata aaattcgaag
     6061 ggattgccgg gatggctgat tcagagctga agatctggcc ggtaccggca aatgagaaaa
     6121 tttttattga aaatgagaag acggcatctg tcatgcgttt tagcctggtc gacccgctgg
     6181 gggagatcct gtttacggat aacatttacc ccggaaagcg atatgaactg aataccggcc
     6241 ggtatcccgg tggcatttat attctgatat ttgagccgtt tgattcaaaa atcaaatcat
     6301 acagaaaaat aataattgaa taaacatatg aagaagaaaa gtttattgct cattttttca
     6361 ctattcactc tcttcggttt ctgccagcag gaggttttct ggtttccggc aaaaggcagg
     6421 gtctatggcg atgtgatgcg tatcatggat gataataatc cctatgtcag gaacaggatt
     6481 cagggtttta atgaaggaga tatcaacgta aatcctgaat attggttcaa tggatttaaa
     6541 gcgcccgcca atgattttga atggtctaac cagagcgggg tttattattc gcaactggat
     6601 tatccatcca ttctggcaga taaggatgtc accacgatta tcctggattt tgtaaccgta
     6661 tgggatgagc agttctatga tacggctgaa tattattatt caaagccggt catggagtgt
     6721 ttgaccgtca acaatggcag ttattcggaa aacatgctgc tggtcaatcc gacagaggtg
     6781 acgcttgatt tcaggttata tgcctcattg ttcaacttgc ccgcaccgac ggatggtgat
     6841 gacaggatag acgggatagt ggaagttatc gatggaaatg ttaaaccgat taatatagcc
     6901 gatacccgta acgggactta tattcacggt gttgatctct cctctttgtc gtacggcttg
     6961 catgaacttg aatattatgc ttacggccag aaatcggatg tgaatcttcc cgatgagttc
     7021 tctgataaaa tcaagatcag gatcctgatc tttgatttta aactccgtta cgatgaggac
     7081 tatgccgtgt gcaaatgcga tacggcgtac gacctggaag gaaggccgga aggggggatt
     7141 ttctcgggcg aatgcgtagt cggatcgtct aatgtgttta atcccgaact gactcagaat
     7201 gcctccaccc ttatcaccta taagtacccg gtggatggaa tgtattacaa cgtggagcgg
     7261 acagtttcct ttgatccgct tccggtcatt gccgtcgatg ccacaacgct ggccggtttt
     7321 ccgcacgaag cctgcggctt tgaacacggc gcggtttatg aagtgaccgg gacggggttt
     7381 gaagatttgt tcagacttcc gcctttagga ataattaaac agtttcatta tgacgggaat
     7441 aaattgacca ttgactggaa aggagagggc gacggctcga tatatgtgac agccctgagc
     7501 gataagggtt gttccgccag cctggaacat atggtgcaca tcacattcaa ccgggcacct
     7561 gaagacagcg cttatgttac cttatatgac agaatgcttt tctgcaatgc cgacacggcc
     7621 agcgttaaag tattcgactg gtatggtgtg attgggggaa gtaatgtgtt ttatgaaagg
     7681 accacgaatc cctattacgt cctcgacttt atacccgccg caggtgactc tttttacgtg
     7741 ctgaccgccg acgatacgac cagttgcagt acccattcgc atatctatac caaacctgcc
     7801 gtccccaaat cggacctgat cggggaaggt gcagaacaac cggctgttaa gcttttcccc
     7861 aacccggtca gaggggatgt aaactgtgaa atcctgaagc ctgtgaagga aggaaggatc
     7921 atcataaccg atgtgcttgg ccgccagata agcaggatca accatgacgg attccttccc
     7981 ggggaagtgg tcaggctgga aacaggtgat tttaaaagcg ggatctatta cgtggtattt
     8041 tcaggtaata acctgtcagg atcctataaa ttcattgttt attaaaatct aaaataaccc
     8101 atgatggaca atcgcgttaa atatacacta ttcatcctgc tgacctttgc agcccttaac
     8161 ggattcagcc aaaagatccc cgcgaaactg gaggccgagg taagcgatca ggtctcagaa
     8221 actattgcaa agtttacaag gcagatcagt agcatttaca cttcggccgg cgaaacttct
     8281 ttccggtttg atagcgttgc actcaatcgg acccgtacca tgttctataa atataatgac
     8341 ccctctttca aacatatcag tcccaatatt attgatcctt caaaagaatt cggactatca
     8401 tcatttccaa aagatacaaa tattctgcct gaaaatttta ttgttcccat cgaccaattc
     8461 ctcgactcac tggcagcctg gtattgtaac gacagttgca ttacaactct cgaactgggt
     8521 tatatggatc cgaaaacaga ttggaataat tattatattg atcctcagtt gttatgcctt
     8581 caggtctttt ataaatt
//
LOCUS       DAOQXS010000045         8593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8701_length_8593_cov_3.888616, whole genome shotgun sequence.
ACCESSION   DAOQXS010000045 DAOQXS010000000
VERSION     DAOQXS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8701_length_8593_cov_3.888616"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3370
                     /locus_tag="PKM34_02325"
     CDS             <1..3370
                     /locus_tag="PKM34_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82448.1"
                     /translation="LDLSAYTSVNVFYEHYFRYFGPNSGWGRLYYSINDGATWTQLGS
                     TISSSLNGADISIDATTQVAGQSRVRFKWNFIGYGYYWAIDDISITGTGPAVWTGATS
                     TDWATATNWSTGAVPTNATNVVIPTTATNWPTLSGDFTAGTTSDKITLYGEAQMNVNG
                     NVNIPAGETLTFTGDGQISLTGNWTNQGNFIPGQGSVRFTGGSPSTVTTSDNSITNFQ
                     RSTFTKGMTDLVSGYSTGPSGNDAASNVNIGFTFNYLGTNYTQARICTNGWMTLNLTG
                     STSATNADLFTTTAPSTTLAPWFDDLTVDNSTSFIRYKTEGIEPYRVFTVEWKTLTTY
                     RTTATARISFQVKLYETTNIIEFHYGTLTAGTHSGSESASAGLEDATGGPGYFLEATT
                     GSSTTGNSNLVSTTQWPLVNYRFSPPVQNFHHLVIDKTSSSLTLAQNSTIRGNLEIAG
                     GEFIGPGNVNKELRIYGDWINNGTFTQGAGTVMFSGSSAQTIGGNTATNFYNLVFGNS
                     AGFALQNNLTAGNIMTMTAGNIDCGADTLILGTSTSATGILNRTSGHIAGCFKRWIAA
                     STVTPVDFPTGAGNAYRNARITFTNNTGGSLTARFEPGDPGNNSGFPLTEGGITINEN
                     NLYTEGSWTLTPTTLSSTNYALELTAEGFSTMGTPDETVRILKRTGDSDPWMLNGTHV
                     NGTATVAKRSGLSGFSRFILAKPCNFNISGNITYYNQANTPLTSGITVKLYQNDTQLG
                     TDYTVTGGTYQFTGLCPGTYQVRISSNRPVDGSINSTDAAQVNYWGVNSYEIEKVRFY
                     AGDVTGGTYYINSTDALRIQANFVNGTSFDRPIWTFWKAGETINSNSTPSESYASVTI
                     SGGDITANIYGLCTGDFNRSFIPGARNDGENQVEMIHDGEISVIPMQEFELPVRVASG
                     CTVGAVSLAMILPSDLFIVKDIVMMNENGQAGWAFNSDELRVGWFTADPVQLAAGDKL
                     LTLKLMMKDVAGILTNLRVSLSPGLLNELADENFIPVDHAVVVTDEISVKMTGIGDTV
                     ESEMLTLSCYPNVTGEMTSIEYSIPRQGRVKLEISNIPGQKVELLADQVQKKGNHKLS
                     YDVTALQTGTYFVVLSYESSGRNMVKSALLVVSR"
     gene            complement(3413..3979)
                     /locus_tag="PKM34_02330"
     CDS             complement(3413..3979)
                     /locus_tag="PKM34_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82449.1"
                     /translation="MTNDRRPTTDDLTTILAMGSERVVADLAVNVLEKTPEAFGDLLE
                     LCWLEQYPLSMRAARVAQLYCEKHPEAIYPFLDEAVEKTLKSKIGGVRRNFLKIFAEF
                     IDIQRISNQGHLLNTCFEWLLDARNTPAIRIHAMGLIYKMSLHEPDLLRELAATIEIV
                     MEEGETSLKTCGRKMLGRISRQSAVGSR"
     gene            complement(4074..4961)
                     /locus_tag="PKM34_02335"
     CDS             complement(4074..4961)
                     /locus_tag="PKM34_02335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ82450.1"
                     /translation="MMNGDIDSGKRSFELRSLGAIGFDTVYKAFSLAFQDYEIRLNRS
                     QLMAMLHRRGFDPGLSFAAFDGDDITAFTLNGIGTFNGIPTAYDTGTGTLKEFRGQGL
                     ATKIFEYSIPFLKEKGVEQYLLEVLQHNTGAVSVYKNLGFEVTREFNYFIKNTNEIKD
                     EHSIHELPFSVRQTGLDELEAASEFWDFHPSWQNGFESINRSAGEFVCLGAFHEHRLA
                     GYCIFEPLTGDVTQIAVSRPYRRKGIGSFLLREMIGRNRSEVLKIINTEISCESITRF
                     LASRNIEPTGRQFEMIRKL"
     gene            complement(5085..5543)
                     /locus_tag="PKM34_02340"
     CDS             complement(5085..5543)
                     /locus_tag="PKM34_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNQ82451.1"
                     /translation="MAFYQLTKKQFVPASIEEVWDFISSPKNLKEITPEYMGFDITSE
                     NLPEKMYAGMIISYKVRPLMRIPMTWVTEIPHVVDKQYFVDEQRSGPYAFWHHQHLIE
                     PYENGVMMTDIVSYKPPLGFLGSIANALMIRKQLEGIFAYREKALKERFP"
     gene            complement(5704..6927)
                     /locus_tag="PKM34_02345"
     CDS             complement(5704..6927)
                     /locus_tag="PKM34_02345"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HNQ82452.1"
                     /translation="MMTYTESYHTLKSIFSTSQKDKTYNGTEIHEFPFISSSCGILPG
                     NPDHFCEKHHQHLSTSFSFYNDVGTIRDDVHIKDIEVKENQAFNYNIIRPAGEGKCRK
                     VIFLFHGFNEKDWTKYLPWAYALCSGTQSAVVLFPIAFHMQRAPKYWSSKREMFRLSE
                     ERRKRFPHIIHSTLSNVAISMRLHSMPQRFIWSGLQTYYDMIRFIEECRQGRHPDIDL
                     NFEFNIFAYSIGGFLAQILKLTNFKGYFEDTKVCLFCGGAAFNRLSPVSKFILDSEAN
                     VALYSFLVEHFDKMLDQDHLLHHYIRDDHPEGRVFYTMLDYRRSRSFREELLKKYEKW
                     FYAISLRKDEVVPSFEIINTLQGAYRDIRIRVDEVDFDRKYLHENPFPVNVAYSGQVD
                     EDFALVFDKACRFLA"
     gene            complement(6983..7291)
                     /locus_tag="PKM34_02350"
     CDS             complement(6983..7291)
                     /locus_tag="PKM34_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82453.1"
                     /translation="MKNAIIIGALVILGATGLILGLSGYHVGEAGDFALGYFSAGQFS
                     AGVFSAGMFSAGIFSVGVFSVGIFSLSVFNVAVYGLGFFLIAWKKHYAKVKVVEEEKI
                     "
     gene            complement(7541..8044)
                     /locus_tag="PKM34_02355"
     CDS             complement(7541..8044)
                     /locus_tag="PKM34_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNQ82454.1"
                     /translation="MKEKSIELLNKAVADEMAAVHQYMYFHFRCDDLGYFLISNLFKR
                     TAIEEMMHVETLAERILFLKGEVEMKAAHDVQKIHDVKQMLELATKMETDSAIDYNNW
                     AMECAKNADSATKKIFEALVDDEERHFDQYDKELDNLAKFGDNYLALQSIENSKNIAA
                     AGGRPAE"
     gene            complement(8131..>8593)
                     /locus_tag="PKM34_02360"
     CDS             complement(8131..>8593)
                     /locus_tag="PKM34_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNQ82455.1"
                     /translation="QFVAWFKWSNLGLLIAVKGADILQSADIGLIPLVILFVILSGFI
                     NLFMGSASAKWAIMGPVFIPMFMLLGYSPELSQAVFRIGDSVTNVISPMMSFFALIIV
                     YYQKYEPKAGLGTLIATMLPYSVTFFLAWTALLIIWILFGLPLGPEAGLYY"
BASE COUNT         2188 a         2268 c         2051 g         2086 t
ORIGIN      
        1 gcttgacttg tcagcttata cttcggtcaa tgtattttat gagcactatt ttagatattt
       61 tggcccaaac tcgggttggg ggcgtttata ctacagcatt aacgatggcg ctacatggac
      121 gcagctaggc agtacaattt ccagttcact taatggcgcc gatatcagca ttgatgcaac
      181 gacacaagtc gcggggcaat cccgggtgcg gttcaaatgg aatttcattg gttatggtta
      241 ttactgggcc atcgacgata tcagcatcac cggcacgggg ccggcagtgt ggaccggcgc
      301 cacctccacc gactgggcta ccgccaccaa ctggagtacc ggggctgtac ctacaaacgc
      361 aacaaatgtc gtcattccaa caacggcaac gaactggccg acgctttcgg gcgactttac
      421 agccggcacc accagcgata aaatcaccct ctacggtgaa gcccaaatga atgtcaacgg
      481 caatgtgaac atcccggcag gggaaacgct gacttttacc ggcgatggcc agatcagcct
      541 gaccggaaac tggacaaacc agggaaattt catccccggg caggggagcg tacgattcac
      601 cggcggttcg ccatctaccg taaccacaag cgataattcc attactaact tccagaggag
      661 cactttcaca aaagggatga ccgacctggt ttcaggttat tcgacgggac cctcgggtaa
      721 cgatgcagca tctaatgtaa atatcggttt taccttcaat tatctcggta caaattatac
      781 ccaggcgcgg atctgcacca acggctggat gacactgaac ctgaccggct cgacctcagc
      841 gacaaatgca gatttattca cgacaacagc cccatccacc acccttgccc cctggttcga
      901 cgacctcacc gtggataatt ccacatcctt catccgttat aaaacggaag ggattgaacc
      961 ctaccgggtc ttcacagtgg aatggaaaac gctgaccacc tacaggacca ctgcaactgc
     1021 ccggatcagc ttccaggtaa aattgtatga aaccacgaat atcatcgaat tccattacgg
     1081 taccctgact gcagggacgc acagcggaag tgaaagcgca tcggccggac tggaagacgc
     1141 taccggcggg cccggatatt tcctcgaagc cactaccggt tccagcacta caggcaattc
     1201 caacctggtc agcaccaccc aatggccgct ggtcaattac cgtttctccc cgccggtaca
     1261 gaatttccat cacctggtca tcgataaaac cagttcatcc ctcaccctgg cccagaattc
     1321 gaccatcagg gggaacctgg agattgccgg cggagaattc atcggacccg gcaatgtgaa
     1381 caaggagttg cggatatatg gagactggat caataacggc acatttacgc agggcgccgg
     1441 tacggtaatg ttttccggct cgtctgctca aaccatcggc ggtaacacag ccacgaactt
     1501 ttacaacctt gttttcggca attcagccgg cttcgcgctt caaaacaacc ttactgcagg
     1561 caatatcatg accatgacgg ccggcaatat cgactgcggc gccgatactt tgatactcgg
     1621 gacttcaact tcagcaaccg gcatattgaa ccggacctca ggccatatcg cgggatgctt
     1681 caaaagatgg attgccgcct ccacggtaac accggtggat ttcccaaccg gggctggcaa
     1741 cgcttaccgc aatgccagga tcaccttcac caataacacc ggcggttcgc tcacagcccg
     1801 gttcgaaccc ggcgatccgg gcaacaacag cggatttcca ttaaccgaag gaggtatcac
     1861 gatcaacgaa aacaacctct acaccgaagg atcctggacc ctcactccca caacgctttc
     1921 cagcacaaac tacgccctgg agctcaccgc cgaagggttt tcaaccatgg ggacccccga
     1981 tgaaacggtc aggattctga agcggacagg agattctgac ccctggatgc tcaacgggac
     2041 ccatgtgaac ggaactgcta cggtggccaa acgctccggc ctcagcggct tttcccggtt
     2101 catccttgcc aagccctgta atttcaatat ttccggaaat atcacttact acaaccaggc
     2161 caataccccg ctcacatcgg ggattaccgt aaaactttac cagaacgata cccagctggg
     2221 taccgattat accgttaccg gcggaaccta ccagttcacc ggcctgtgcc cgggcacata
     2281 ccaggtcagg atctcgtcga accggccggt tgacgggagc atcaacagca ccgacgcggc
     2341 acaggtcaat tactggggcg tcaattctta cgaaatcgaa aaagtcaggt tttacgccgg
     2401 cgatgtcacg ggcggcactt actacatcaa cagcaccgac gcgcttcgca tccaggcgaa
     2461 ctttgtgaac ggcacttcct tcgaccgccc catctggacc ttctggaaag ccggcgaaac
     2521 catcaacagc aacagcaccc cttcggaatc ctatgccagc gtgaccatct ccggcggcga
     2581 tatcaccgca aacatctacg gcttgtgcac cggcgacttc aaccgcagtt ttattcccgg
     2641 tgcaagaaat gatggcgaaa accaggtaga aatgatccac gacggcgaaa tatccgttat
     2701 acccatgcag gaatttgagc tgccggtgcg ggtcgcctcc ggatgcacgg tcggggcagt
     2761 ttcattggct atgattctgc cttctgatct ttttattgtg aaagacattg tcatgatgaa
     2821 tgaaaatggc caggccggct gggcgttcaa cagcgatgaa ttgcgggtcg ggtggttcac
     2881 ggccgatccg gtgcaactgg ctgccggcga taagcttctt accctcaaac tgatgatgaa
     2941 ggatgtcgcc ggcattctta ccaatctccg ggtctccctg tctcccggtc tcctgaacga
     3001 gcttgccgat gagaatttta ttccggtcga tcatgcggtc gtggttaccg atgagatttc
     3061 agtaaaaatg actggaatcg gcgatacggt tgagtcagaa atgttaaccc tttcatgtta
     3121 cccgaacgtg accggagaaa tgacctccat tgaatactca attccccggc agggcagggt
     3181 aaagctggaa atcagcaata ttcccggtca gaaagtggag ttgcttgccg accaggttca
     3241 aaaaaagggg aatcacaaat tgtcatatga tgtaacggcg cttcaaacgg ggacttactt
     3301 cgttgtactt tcgtacgaaa gcagcggccg gaacatggtg aagtcagctt tgctggtcgt
     3361 tagtcgttag tcgtcggccg ttagtcacca cctcctttgt ccaactgccc gactacctgc
     3421 taccgactgc cgactgccga ctgatcctcc ccagcatctt cctcccacac gtcttcagcg
     3481 aggtctctcc ttcttccatg acgatctcta ttgttgcggc cagttcccgc agcaggtcgg
     3541 gttcatggag gctcattttg tagatcagtc ccatggcatg gatccggata gccggcgtat
     3601 tgcgggcatc gagcagccat tcgaagcagg tattcagcag gtgtccctgg ttgctgattc
     3661 gctgaatatc gatgaattcg gcaaatatct tcagaaagtt gcggcggacc ccgccgatct
     3721 ttgacttcag cgttttctcc accgcttcat ccaggaaagg atagatggcc tccgggtgct
     3781 tttcgcagta gagctgggct acgcgcgccg cccgcatcga cagcgggtac tgctccagcc
     3841 agcatagctc cagcaggtcg ccgaaagctt ccggtgtttt ctccagcaca ttcacagcca
     3901 ggtcggccac cacccgttcg gagcccattg caagaatggt ggtaaggtcg tcggtcgtcg
     3961 gtcgtcggtc gttagtcatg gattgctggt cggttgtttt ttaaggagat acctgaagaa
     4021 ttggttgttg ttcgccggat atgatcgcta ttcctgttat caagcaaaga agatcaaagc
     4081 tttctgatca tttcaaattg ccggcctgtg ggttcaatat ttctggaggc taagaaccgg
     4141 gttatggatt cacaggagat ttccgtattg ataattttca ggacttcact ccggttacgc
     4201 ccgatcattt cccggagcag gaatgaaccg atcccctttc gcctgtatgg cctgctgacc
     4261 gcaatctggg tcacatcccc cgtaaggggc tcaaaaatgc aataccctgc cagacggtgt
     4321 tcatggaaag ctcccaggca aacaaattcg ccggccgatc tgtttatgga ttcaaaaccg
     4381 ttctgccacg atggatgaaa atcccaaaat tctgatgcgg cctctaattc atctaatcct
     4441 gtttgtcgaa cagaaaaggg aagttcatga atagaatgct catcctttat ttcattggta
     4501 ttcttaatga aatagttaaa ctcgcgggtc acttcaaatc caagattctt atacaccgat
     4561 accgcccctg tgttgtgctg cagaacctcc agcaggtatt gttcaacgcc cttttctttc
     4621 aaaaatggga tggaatattc gaagatcttc gtagccagcc cctgtcccct gaactccttg
     4681 agcgttcccg tccccgtatc ataggccgtc gggatcccgt tgaaagtccc gatgccgttc
     4741 agggtaaagg ccgtaatgtc atctccgtcg aacgcggcaa acgacaaccc gggatcgaaa
     4801 ccgcgcctgt gcagcattgc cattaactgt gaacggttca gccggatctc gtaatcctga
     4861 aaagccaggc tgaaggcttt gtaaacagta tcgaaaccga tagcaccgag agaccgcaat
     4921 tcaaaagatc tctttcccga atcaatatca ccgttcatca taaaattatt tccttttttc
     4981 ttttcttttt ttccgccaaa ataaaacaaa gccggaaaat actcaaatcc tggagataaa
     5041 tatacggtgt tctgcgtcgg gcttagtggt ttttgttaaa gctttcaggg aaacctttct
     5101 ttcaaggctt tttcgcggta agcaaagatc ccttccagct gtttcctgat catcaaagca
     5161 ttggcaatgc tcccgagaaa tcccagcggc ggcttgtacg aaacgatatc ggtcatcatc
     5221 accccgttct cataaggctc aatgagatgc tggtggtgcc agaaagcata gggcccggaa
     5281 cgctgttcat cgacaaaata ttgcttatca accacatgtg ggatctccgt cacccacgtc
     5341 atcggaatcc tcatcagcgg cctgacttta taactgatga tcatcccggc atacattttt
     5401 tccgggaggt tttcagaagt gatgtcgaat cccatgtatt cgggggtgat ctctttcagg
     5461 tttttgggag atgagatgaa atcccagact tcttcgatgg aggccgggac gaattgtttt
     5521 ttagtgagtt gataaaaggc cattttactg ttttaagaaa tgtaaccaac taattatcaa
     5581 ttctgaacaa tataagtggc caaatgttta aaaaaatttg agatgtggct acttataatc
     5641 tctgtttttg aaaaaattcc ggtcatcatc accccgttct caaaagattc aatgagatac
     5701 ctgtcaagcc aggaaacggc aggctttatc gaataccagg gcaaaatctt catcgacctg
     5761 cccggagtaa gcaacattca ccgggaaagg gttctcgtga agatatttac ggtcgaaatc
     5821 aacctcatcg acccgtattc tgatgtcgcg gtaagccccc tgcagggtgt tgatgatctc
     5881 aaacgacggg acgacctcgt cttttctcag ggaaatggca tagaaccact tttcgtattt
     5941 tttcagaagc tcttcgcgga acgatcttga tctccggtag tcgagcatgg tatagaatac
     6001 ccgcccttcc gggtggtcgt cacgaatata atggtgcagc agatggtcct ggtccagcat
     6061 cttgtcgaaa tgctcgacca ggaacgagta cagggcaaca ttcgcctcac tgtcgagaat
     6121 gaacttcgac acgggcgaca ggcggttgaa agcagcgccg ccgcaaaaca ggcaaacttt
     6181 cgtatcctcg aagtagcctt tgaaattggt cagcttcagt atctgtgcca ggaacccgcc
     6241 gatgctgtat gcgaaaatat tgaattcaaa gttcaggtcg atatcgggat ggcgtccctg
     6301 cctgcactcc tcaatgaacc ggatcatatc gtaataggtc tgcaatcccg accagataaa
     6361 ccgttgcggc atggaatgca accgcatgct gatcgccaca ttcgacaaag tcgagtggat
     6421 gatgtgggga aaccgcttcc tgcgctcttc gctgagccgg aacatctccc gcttgctgct
     6481 ccagtatttc ggcgcacgtt gcatgtgaaa cgcgatggga aacagcacca cggcgctctg
     6541 tgtcccgctg cagagcgcat aagcccaggg caggtacttt gtccagtctt tttcgttaaa
     6601 tccgtgaaac aggaaaatca ccttccggca tttcccttcg ccggccggcc ggattatatt
     6661 ataattaaaa gcttggtttt cttttacttc tatgtctttt atatgaacat catcccggat
     6721 ggtgcccaca tcgttataaa acgaaaaaga agtggaaaga tgctgatggt gcttctcaca
     6781 gaaatgatca gggttccccg gcaggattcc gcaggaggaa gaaatgaacg gaaactcatg
     6841 aatttccgtt ccgttatatg ttttgtcttt ttgtgaagtt gaaaagatag atttcagcgt
     6901 gtgatagctc tcggtgtagg tcatcatttt ccggttagga taaaacagga atcattctta
     6961 tcatcaaacg cccgaaaagg atttatattt tctcctcctc caccacttta acttttgcgt
     7021 aatgcttctt ccaggcgatc aggaaaaagc ccaggccata tacagccacg ttgaatacgc
     7081 tcagcgaaaa gatgcccaca ctgaatactc ctactgaaaa tatccctgcg gaaaacattc
     7141 cggccgagaa cacgccggcg gagaattgtc cggcagagaa atacccaagg gcaaaatcgc
     7201 ccgcttcacc cacatggtaa ccgcttaatc ccagaataag cccggtggcc ccaaggatca
     7261 ccagcgctcc gataataatt gcatttttca tttttttgtt ttttaggtta atgaataaaa
     7321 atttgatggg gcaatattac actcatcccg gaagcctgta aaggaatttg ggatgaatgg
     7381 aaggatttgt gacgaaagga ctatccccgg gacgaaatgg gcatcaaccg ggacaaaatg
     7441 cattgatttt cccgcccgat tcatacaata cacagtaccc gaatgccatc tttcaagaca
     7501 tccggacaaa cactttgaac tttgaacttt gaactttgaa ctattcagcc ggtcttccgc
     7561 ctgcagcggc aatgttcttg ctgttctcga tagattgcaa agcgagataa ttatcgccaa
     7621 acttggccag gttatccagt tccttgtcgt attggtcgaa gtgccgttct tcgtcatcga
     7681 ccagggcttc aaagatcttt ttcgtggcgg aatcggcgtt tttggcacat tccatggccc
     7741 agttgttata gtcgatcgcg ctgtcggtct ccatctttgt ggccagctcg agcatctgtt
     7801 tcacatcatg gattttctgc acatcatggg ctgctttcat ttcaacttct cctttcagga
     7861 aaaggattcg ttcggccagg gtctccacat gcatcatctc ttcaatggca gtgcgcttga
     7921 ataggttgga gatgaggaaa taccccaggt cgtcgcaacg gaaatggaaa tacatgtact
     7981 ggtgaacggc ggccatttca tcggcaacgg ctttgttcag taattcaatg cttttttctt
     8041 tcatggtcaa tatatcttta ggatttgatt tatgcagggt gtaaagatac taaaaaaaaa
     8101 gggatggcgt ccctagggac gccatccctt ttagtagtac aacccggcct caggccccag
     8161 gggtaaacca aataaaatcc aaatgatcag cagggcggtc catgccagga aaaaggtcac
     8221 ggaatagggc agcatggtcg caatcagcgt cccaaggcct gctttgggtt catatttctg
     8281 gtaatagacg atgatgaggg cgaaaaagct catcatgggc gagatcacat tcgtgacact
     8341 gtcgccgata cggaatactg cctgggatag ctccggggaa taccccagca gcatgaacat
     8401 cgggatgaat accgggccca tgatggccca tttggccgag gcactcccca tgaacaggtt
     8461 gatgaacccg gataaaatga caaaaaggat gaccagcggg atcagcccga tatccgcgct
     8521 ctgaagaatg tcggcccctt ttacggcaat cagcaggccc aggttgctcc acttgaacca
     8581 cgccacaaac tgt
//
LOCUS       DAOQXS010000046         8532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8776_length_8532_cov_3.539342, whole genome shotgun sequence.
ACCESSION   DAOQXS010000046 DAOQXS010000000
VERSION     DAOQXS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8776_length_8532_cov_3.539342"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..940)
                     /locus_tag="PKM34_02365"
     CDS             complement(179..940)
                     /locus_tag="PKM34_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82456.1"
                     /translation="MKTRIFSIAFIFFLGGLFIVGTSRCSTARKTADSGQPEIINTNI
                     NGQGLSIRINFTKGESHNHPLMAFWLEDKDGKYIETIYISESIGKGIFQHGDRSKGRW
                     QPGEVRRPAALPYWGHKRGIQAPDGYFIPTVALPMPDAVTGPTPKGDFILKSKSTEQA
                     PRIFKVMMEINQSWDWNEFWTNSKYQGDVNYMTSSQPSLVYEAVINLDSPVKEYEMKP
                     VGHGHYSGADGSLNPDLSTITTAGEIAESVKVTVE"
     gene            complement(955..3345)
                     /locus_tag="PKM34_02370"
     CDS             complement(955..3345)
                     /locus_tag="PKM34_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ82457.1"
                     /translation="MKRKIISSLSVIFALIPFMIHAQQGSVKGRIFDESNNEPIAFAN
                     IIVFGTTIGSTSDLDGNFIFTGLEPGFIKLAASAVGYESKVTIDFQVTNSKTSFMDIP
                     MKPKEFELQEVVIKASPFKRLEESPVSLRTLQISEIEKNPGGNRDISRVIQVLPGVSS
                     TPAFRNDVLVRGGGGNENAFYLDGIEIPNLNHFSTQGSSGGPVGIINADFIREVDFYS
                     GAFPADRGKSLSSLLDMKLIDGNKEEWIFRGSLGASDLALTTNGPVSDNFTFLFSYRR
                     SYLQFLFDALGLPFLPNYDDFLAKYKIQFDQKNELSVIGLGSIDRFELNTGIKDPDES
                     QAYILGYLPVQEQWSYTIGAVYKHYHKNGFTDVILSRNMLDNRSFKYRNNDENDTLNF
                     NYNSQESENKLRVEHTSRRGAFKYTYGGGLELARYTNSTFQRLYANDVYTPVNYDSQL
                     RMFLWNVFGQVSKNFFDDRLVLSLGLRMDANDYTPYMANLLNQVSPRFSASFGLTDIW
                     FLNFNTGRYYQRLPYTTFGFRDNNGELVNKRDDLRNLSADHVVFGFEVLPERDAKFSI
                     EGFYKYYRNYPFSVRDSINIASKSAGYGTFGDEQVISVSDGRAYGFEVLAQDKSLFGF
                     NVILSYTFVVSKFTDKDDKFVPSAWDNRNILNLTVLKSFKRNWDIGAKWRYVGGAPYT
                     PADLEFSSYVPAWDARGREYPDNSRFNELRLKAFHQLDLRVDKTYFFKKWSLNLYIDV
                     QNVYNFQAENPPNYTNLDKDGNKVYQDPPTNQFYQLRALNSESGTVLPTLGIIVEF"
     gene            complement(3409..4719)
                     /locus_tag="PKM34_02375"
     CDS             complement(3409..4719)
                     /locus_tag="PKM34_02375"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNQ82458.1"
                     /translation="MNNTEINLFWFRRDLRLDDNAGLFHVLSSGLPVLPIFIFDDDIL
                     LKLDNKEDARVTFIYDEVLRIKQEIESRGGSLLIKRGRPAGIFRELTGRFRISKVFAN
                     HDYEPYARNRDDRVGKLLSEQGIRFITFKDQVIFEKDEILKDDGKSYTVFPPYSRRWK
                     SALSGSSFTKHNSAGLTGNFLKMMPMDVPALSSLGFNRSRLTFPPREPDAGILQHYHR
                     DRDFPALQGTSRMGPHLRFGTVSIRKLAEMAHKTNEVFLNELIWREFYMMILWHFPHA
                     AERAFKPQYDRIAWRNDEQQFRAWCEGRTGYPIVDAGMRELNATGYMHNRVRMITASF
                     LARHLLIDWRWGDAWFASKLLDFELSSNNGGWQWAAGTGCDAAPYFRVFNPVLQAEKF
                     DPEKNYIRQWVPEYGTGSYPPPIVEHSRARLLAIETYQKALKPD"
     gene            complement(4712..5635)
                     /locus_tag="PKM34_02380"
     CDS             complement(4712..5635)
                     /locus_tag="PKM34_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNQ82459.1"
                     /translation="MKTLIIGATGFIGRELVKDLADHGHTVVAVTRNSGMARAILGKK
                     CSIVEWDGISVSSLVHYMAGCEAVVNLAGENIASGRWTEKRKEALVASRTTVGRKLAE
                     AILRLTSPLPVLVQGSAIGIYGTDVDSASDESLAPGSGFLANLVVEWENSVAEALPKV
                     KRLAWARTGLVLGRDGGLLEKMLLPFRFHSGTLLGSGRQWMSWIHLSNEVRAIRFLLE
                     NNTSSGHYNLTAPMPVKMEEFIRALSRITGKPAWVRVPGAVIKALMGEMAAETVLASQ
                     NALPARLLRDGFTFDYPRLEDALTNLLTNNE"
     gene            5806..7281
                     /locus_tag="PKM34_02385"
     CDS             5806..7281
                     /locus_tag="PKM34_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ82460.1"
                     /translation="MEPTASVVGRKNPPEPTASVVGLQTQHINHNPPHLFAKGVNLPK
                     KSVTLKTSVTNKDIWLIAYPIILGSVAQNIINITDTAFLGRVGQVALGAGAIGGIFYL
                     VASMLAWGFGIGSQIIIARRHGEQAFGEIGRTFRHGFFFMLPLTLTLFSLMKFFSGDL
                     LSLIISSEAVYDSLQEYLGYRSWGIFFAGVNMLYRGLYIGIARTKVITYTTFILAFTN
                     AFLDYCLIFGNLGFPELGIGGAALASTLSEAVATVYFTVYTFFILDGRKYRITARSRF
                     DEQLYRRIIRISLPVMMQHFISMVAWLVFFLFIEKLGEMALAVSNIVRSFYVILMIPM
                     WGFSSATSTLVSSLIGQGRQGEVMSLVYKVARMCAFGVMIVAALGALFPDYALMIYTD
                     DAGMIKASLPALYVVNISVLFLAVGFVFFSAVSGTGKTQVSFLIELAVIVFYLVFSYL
                     LAVVWRAEVYVVWMAEFFYAFMLGTLSYLYLKRGKWKSVRI"
     gene            complement(7333..7554)
                     /locus_tag="PKM34_02390"
     CDS             complement(7333..7554)
                     /locus_tag="PKM34_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HNQ82461.1"
                     /translation="MTRSELEAILKEGVNSCANPQGWANLAELGTYLRQRGLQYGKLS
                     RFLQDYQNLIETRMDTNITPPAVYARLRQ"
     gene            complement(7664..8128)
                     /locus_tag="PKM34_02395"
     CDS             complement(7664..8128)
                     /locus_tag="PKM34_02395"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82462.1"
                     /translation="MVSNYFDIYLWKMNTNLEDDTLYTQSMTYDSLCPYEIQSDTMDL
                     DCDLFVNIDELPTKEEYESTVKISPNPAEDWVALTLPDIVASEKVEIAVYDVFGREVE
                     TGRRGDKEMGRQGEVPVNRMVVLDVSGYPPGMYVVVVTDRKERRYSGKMVIR"
BASE COUNT         1909 a         2209 c         2216 g         2198 t
ORIGIN      
        1 tcaaatggtt gtcctcataa taacacttgc gcagtttgtc gccattggca tcgtaaagat
       61 aggttatttt gagattgttt tccggggagg atatatcttt tgagactcca tctcgattcg
      121 ggcagatggc gtctcgctaa aaatgagatg gagtctcata gaaattgaga tggagtctct
      181 attcaaccgt taccttcacg ctctccgcaa tctccccggc ggtcgtgatg gtgctgagat
      241 cggggttaag gctcccgtcg gcgccggaat aatggccatg acccaccggt ttcatctcat
      301 attcctttac cggggagtcg aggtttatca cggcttcata aaccagcgag ggctggcttg
      361 aggtcatgta gttgacatcg ccctggtatt tgctgtttgt ccagaattcg ttccagtccc
      421 acgactggtt gatctccatc attactttga atattcgcgg agcctgttct gttgatttac
      481 ttttcaggat gaaatctcct ttgggagtcg gcccggtaac cgcgtcgggc atcggcaggg
      541 ctaccgtcgg aataaagtat ccgtcgggtg cctggatacc ccttttgtgc ccccagtatg
      601 gcaaagcagc cggcctgcgt acctctcccg gctgccacct gcctttcgac cggtcgccgt
      661 gctgaaagat acccttaccg atcgattcgg aaatataaat agtttctata tacttcccgt
      721 ctttgtcctc cagccagaaa gccatcaggg gatggttatg actttcacct tttgtaaaat
      781 tgatacggat tgaaagaccc tgtccgttaa tatttgtgtt gatgatttca ggctggcctg
      841 aatctgcagt ctttcgtgcg gtggagcacc gtgatgtacc cacaatgaac aatccgccga
      901 ggaaaaaaat gaatgcgatt gaaaatattc tggttttcat agttgatccg gtcattaaaa
      961 ttcgacaata attcctagcg tgggcagaac agttcccgat tccgagttca gcgcacgaag
     1021 ctgataaaac tggtttgtgg ggggatcctg gtaaactttg ttgccgtcct tgtccaggtt
     1081 ggtatagtta ggcggatttt cggcctggaa attataaacg ttctgaacat caatatataa
     1141 attcagcgac catttcttga agaaataggt cttgtcaaca cggagatcaa gctggtggaa
     1201 ggctttgagc cgcagctcgt tgaacctgga attgtcggga tattcccggc cgcgggcatc
     1261 ccaggccgga acataactgg aaaactccag gtcggccgga gtgtagggcg ccccgccaac
     1321 gtaacgccat ttggcgccaa tatcccagtt tcgcttaaag ctcttcagaa cggtaaggtt
     1381 gaggatgtta cggttgtccc acgcagaagg gacaaattta tcgtccttat ccgtaaactt
     1441 actgacaaca aacgtgtacg acaaaatcac gttgaatccg aacagcgact tgtcctgagc
     1501 cagtacctcg aatccgtacg cccggccatc cgaaaccgag atcacctgct catcgccgaa
     1561 tgtgccgtac cctgcgctct tgctggctat gttgatcgaa tccctgacag agaacgggta
     1621 gttcctgtag tacttgtaaa agccttcgat gctgaatttg gcatcccttt cggggaggac
     1681 ctcgaatccg aagaccacgt ggtcggcgga gagattgcgg agatcatccc gcttgttaac
     1741 cagttctccg ttgttgtcgc gaaatccgaa agtagtatag ggcaaccgct ggtagtacct
     1801 tccggtgttg aagttcagga accagatatc ggtcagcccg aaggaagccg aaaatctcgg
     1861 ggaaacctgg ttgagcaggt tggccatgta aggcgtatag tcgtttgcat ccattctgag
     1921 accgagagaa aggaccagcc tgtcgtcgaa gaaatttttg ctgacctgcc cgaatacgtt
     1981 ccacaggaac atcctaagct gtgagtcgta attgacgggc gtgtaaacat cgttcgcgta
     2041 aagccgctga aaggtgctgt tggtgtagcg ggccagttcc agtcctccgc cgtaggtata
     2101 tttgaacgct ccccggcggc tggtgtgctc cacgcgcagc ttattctccg attcctggga
     2161 gttatagttg aaattcagtg tgtcgttctc atcgttgttc ctgtatttga acgaccggtt
     2221 atccagcatg ttccggctca ggatcacatc ggtaaagccg ttcttgtgat aatgcttgta
     2281 cacggcgccg atggtatacg accactgctc ctgcaccggc agatagccaa ggatgtaggc
     2341 ctggctttca tccggatcct tgatcccggt gttcagttcg aaacggtcga tcgagccgag
     2401 gccgataacc gaaagctcat ttttctggtc gaactgaatc ttgtattttg ccaggaaatc
     2461 gtcgtagttg ggcaggaaag ggagacccag tgcgtcgaac agaaattgca ggtacgaacg
     2521 gcggtaggag aataaaaagg tgaagttgtc cgacaccggg ccgttggtgg tcaatgcaag
     2581 gtcgctggcg ccgagcgatc cgcggaagat ccactcctcc ttgttgccgt cgatcagttt
     2641 catgtcgagc agcgagctga gcgatttgcc gcggtcggcc gggaaagcgc cgctgtagaa
     2701 gtcgacttcc cggataaaat cggcattgat gatgcccacc ggaccgccgc tggaaccctg
     2761 cgtcgagaaa tggttcaggt tcgggatctc gatcccgtcg aggtagaacg cgttttcgtt
     2821 gccgccgccg ccgcgaacaa gcacatcgtt acggaatgcc ggtgtggacg acacgcccgg
     2881 caaaacctgg ataaccctgg aaatgtcgcg gttaccgccc gggttctttt cgatctccga
     2941 gatctgcagc gttcgcagcg aaacgggact ctcctccagc cgcttgaacg gcgacgcctt
     3001 gataaccacc tcctgcagct cgaactcctt cggcttcatc gggatatcca taaatgatgt
     3061 tttcgaattg gtcacctgga aatcgatggt caccttcgat tcatatccga ctgccgaagc
     3121 ggccagcttg ataaatcccg gctccagccc cgtgaaaatg aaattgccat ccaggtcgga
     3181 ggtcgaaccg atggtcgtcc cgaacacaat aatgttggca aatgcaatcg gctcgttgtt
     3241 gctttcgtca aaaatcctcc cttttaccga tccttgctgg gcatggatca taaaggggat
     3301 taaggcgaaa atcacactca atgaggatat tatctttctc ttcatctgtt aataatcttt
     3361 gtttaagttt caggtttggt ttgttaaaca aaagtccgga taaaaagttc aatcgggttt
     3421 caacgctttt tggtacgttt cgatagccag caaccttgcc cggctgtgct ccacgatagg
     3481 gggcgggtag ctccctgttc cgtattcggg cacccactgc cggatataat ttttttcagg
     3541 atcgaatttc tcagcctgca aaaccgggtt gaatacccgg aaatagggcg cggcgtcgca
     3601 gccggtccct gccgcccact gccagccgcc gttgttcgac gacagctcga agtcgagcaa
     3661 ttttgaagca aaccaggcat cgccccagcg ccagtcgatc agcaggtgcc tggccaggaa
     3721 gctggcggtg atcatccgga cccggttgtg catgtagccg gtggcgttca gctcccgcat
     3781 cccggcgtcc acgatcgggt atccggtgcg gccttcgcac caggcccgga actgctgctc
     3841 gtcgttccgc caggcgatcc ggtcgtactg tggtttgaag gcccgttcgg ctgcatgagg
     3901 aaagtgccac aggatcatca tgtagaactc tctccagatc agttcgttca gaaagacctc
     3961 attggttttg tgcgccattt ccgccagttt ccggatactc acggtgccaa accgcaggtg
     4021 cggccccatc cgggaagtgc cctgaagcgc cgggaaatcg cggtcgcggt ggtaatgctg
     4081 aaggataccg gcatcgggtt cccgcggtgg aaatgtcaaa cgcgaccggt tgaatcccag
     4141 ggatgacagg gcaggaacat ccatcggcat catcttcagg aagttgccgg tcaagcctgc
     4201 tgagttgtgc tttgtgaaag atgatccgct cagggcggac ttccatcttc gggaatacgg
     4261 gggaaaaacg gtgtatgatt tgccgtcatc tttcaggatc tcatctttct caaaaataac
     4321 ctggtccttg aaagtaataa accggatacc ctgctccgac aacaatttcc cgacccggtc
     4381 atccctgtta cgggcataag gctcatagtc atgattggca aaaaccttgc tgatccggaa
     4441 ccggccggtg agttcccgga aaataccggc cgggcggccc cgtttgatca ataatgatcc
     4501 tccccggctt tcgatttcct gtttgatccg gaggacctcg tcataaatga atgttaccct
     4561 tgcatcttct ttattatcaa gttttagaag gatgtcgtca tcaaaaatga aaatggggag
     4621 aacgggcagg ccactgctga ggacgtgaaa caggccggca ttatcgtcca gccgaagatc
     4681 tctcctgaac cagaaaaggt ttatttccgt gttattcatt gtttgtcagc agattcgtta
     4741 aagcatcttc cagccgggga tagtcaaatg taaacccatc ccggagcagc ctggccggga
     4801 gggcattctg actggccagc accgtttcgg cggccatttc gcccatcagg gctttgatca
     4861 cggctccggg caccctaacc caggcaggtt tgccggtaat tcggctcagg gcgcggataa
     4921 actcttccat cttcaccggc attggggctg tcaggttgta atggcccgaa gaggtgttgt
     4981 tttcgaggag gaaacggatc gcgcgcactt cgttgctgag gtggatccac gacatccact
     5041 gccgccccga tcccaggaga gtgccgctgt gaaatcggaa cggcaggagc attttttcaa
     5101 gcaaaccgcc atcccggcca agcaccagac ccgtccgcgc ccaggcaagc cttttgacct
     5161 tgggcagggc ctccgctact gaattttccc attccaccac caggtttgcc aggaatccgc
     5221 tacccggagc cagcgattcg tcggatgcgg aatcgacgtc ggtcccgtag attccgatgg
     5281 ccgagccctg gaccagcaca ggcaggggag aggtgaggcg gagaatggcc tcggccagct
     5341 tacggccaac ggtggtgcgg ctggcgacca gggcctcttt tcttttctcc gtccaccggc
     5401 ccgaagcgat attctccccg gccaggttga ccaccgcttc gcatccggcc atgtagtgga
     5461 ccagcgagga gaccgaaatc ccgtcccact ccacgatcga gcattttttc ccgagaatgg
     5521 ccctggccat ccccgagttc cttgtcacag ccaccaccgt gtggccgtga tccgccagat
     5581 cttttaccag ctcccggccg ataaaccctg ttgcgcctat gatcagtgtt ttcattttat
     5641 gatttcattt tcatattcgt ggtccaacga ctaaagccgt tggttgcctg tgatcgtggt
     5701 ccaacgacta aagccgttgg ttgcctgtga tcgtggtcca acgactaaag ccgttggttg
     5761 cctgtgttcg tggtccaacg actaaagccg ttggttccag aattgatgga accgacggct
     5821 tcagtcgtcg gaaggaaaaa cccgccagaa ccgacggctt cagtcgtcgg actacaaacc
     5881 caacacatta accacaaccc gcctcatttg ttcgcaaaag gagtaaattt accgaaaaaa
     5941 tcggttacac tgaagacgtc ggttaccaat aaggatattt ggctgatcgc atacccgatc
     6001 atactcggaa gcgtagcgca gaacatcatc aacatcaccg atacggcctt cctggggcgg
     6061 gtcggacagg tggcattggg ggccggggcg atcgggggca ttttttacct ggtagccagc
     6121 atgctggcgt ggggattcgg gatcgggagc cagatcatca tagcccggcg gcatggcgag
     6181 caggcgtttg gggagatcgg gcggaccttc cggcacggtt tctttttcat gctgcccctt
     6241 acgctaaccc tgttcagcct gatgaagttc ttctccggcg atctgctcag cctgattatc
     6301 agttcagaag ccgtgtacga cagcttgcag gagtacctcg gttaccgctc ctgggggatt
     6361 ttctttgccg gggtaaacat gctgtaccgc gggctttaca tcggcattgc acggacaaaa
     6421 gtgatcacct acaccacatt tatcctggct tttacgaatg cttttctcga ttattgcctt
     6481 atcttcggca acctgggctt tccggagttg gggatcggcg gggcggcgct ggcctcgacc
     6541 ctttcggaag cggttgcgac ggtctatttt accgtttaca ccttttttat cctggatggc
     6601 agaaagtatc gaatcaccgc ccgcagccgt ttcgacgagc agttgtaccg gaggatcata
     6661 cggatatcct tgccggtgat gatgcagcat ttcatctcga tggtggcctg gctggttttt
     6721 ttcctgttca tcgagaagct gggggagatg gccctggccg tttcgaatat tgtaaggagt
     6781 ttttatgtga tattaatgat cccgatgtgg ggattttcat cggcgacaag caccctggtc
     6841 agcagtttga tcgggcaggg gcggcaaggc gaggtgatgt cgctggtgta caaggtggcc
     6901 cggatgtgcg ctttcggggt gatgatcgtg gcggccctgg gcgccctgtt ccccgattac
     6961 gccctgatga tctacaccga cgatgcggga atgatcaaag cttcactacc ggctttgtac
     7021 gtagtgaata tatcggtcct tttcctggct gtcgggtttg tatttttcag cgccgtatcg
     7081 ggaacgggca aaacgcaggt gtcgttcctg atcgaactgg ccgtgatcgt attttacctg
     7141 gtattttcct atttgctggc cgtggtttgg agggcggagg tttacgtggt ctggatggcc
     7201 gagttcttct atgcttttat gctgggtaca ttatcgtatc tctacctgaa aagaggcaaa
     7261 tggaaatccg taaggatttg accgtcagaa aagatattca cccgaaggtg gcaggatagc
     7321 aggcaggcag cattactggc gaagccgggc gtatacggca gggggagtga tgttggtgtc
     7381 catccgggtc tcgatgaggt tctggtagtc ttgcaggaag cgtgacaatt tgccgtattg
     7441 aagtcctcgc tggcgcaaat aggttcctaa ttccgccagg ttcgcccaac cttgcgggtt
     7501 ggcgcagctg ttaacccctt cctttaaaat agcttctaat tcggagcgtg tcattttaaa
     7561 aaaagtttta actgtttctt aacgagaaaa acaaatatac aacaaattgg ttcaaaagta
     7621 aagggttttt tgtatgattt cccgattttg tcggatcggc taattacctg ataaccatct
     7681 ttcccgaata cctcctctct ttcctatccg ttaccaccac cacatacatc cccggcggat
     7741 aacccgacac atccagcact accatccggt tcaccggcac ttcaccttgt ctccccatct
     7801 ccttgtcccc tcgtctcccg gtctccacct cccttccaaa cacatcataa acagctatct
     7861 caactttctc tgaagctact atatccggca acgtcagcgc tacccaatct tcggccggat
     7921 tcggcgaaat tttcacagtc gattcgtatt cttctttggt aggcagttca tcgatattga
     7981 caaacaggtc gcaatccagg tccatcgtat ccgattgaat ctcatagggg cacaggctgt
     8041 cgtacgtcat cgactgggtg tagagggtgt catcttcgag gtttgtgttc attttccaga
     8101 ggtagatgtc gaagtagttg cttaccatgt atgatccgac aactataatt ttatcatcaa
     8161 atgatgtaat aatatctgcc ggggcttctg tttcattatt aacaaggaat ctacgatgaa
     8221 tagaattccc aagtgtgtct attattgcta attcactatg aattaattca ctaggatatt
     8281 tctcgcccca tacaaatcca gttatcaaag tagaatcatt aagtcttgct atacttctac
     8341 ccccaccttg tttgattgta tcacccacta ataagtaact ttcaatttca tttcccgact
     8401 cgtcaaattt gaatatgctc ggaatattat tggttccggg aagtatgcaa taacctatac
     8461 tgtaaaattt tccatatgta tgttctgtcg tttgaaagca aattcctgtc acagaatccc
     8521 attttagatc cc
//
LOCUS       DAOQXS010000047         8383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8960_length_8383_cov_4.249760, whole genome shotgun sequence.
ACCESSION   DAOQXS010000047 DAOQXS010000000
VERSION     DAOQXS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_8383_cov_4.249760"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..60)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(66..335)
                     /locus_tag="PKM34_02400"
     CDS             complement(66..335)
                     /locus_tag="PKM34_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82463.1"
                     /translation="MNEISAMKLDVISGGGRKKDFWDIHELLNHFSIQQMLDLHEKRY
                     PHSHDPSQILKKLTDFNSADDDFNPFCLKGKHWELIKLDLIEWFK"
     gene            complement(332..532)
                     /locus_tag="PKM34_02405"
     CDS             complement(332..532)
                     /locus_tag="PKM34_02405"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ82464.1"
                     /translation="MAIHKLFYNTTTPLLLEILKALMVSDEFRPFRLVGGTALSLQLG
                     HRKSLDIDLFTDRDYDFIDFQA"
     gene            complement(519..980)
                     /locus_tag="PKM34_02410"
     CDS             complement(519..980)
                     /locus_tag="PKM34_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance system antidote protein"
                     /protein_id="HNQ82465.1"
                     /translation="MNNELIIIKGIHPGFYLERELRRRNLGKSKFAISVGEYPQTLVS
                     ITKGKRKMNTSLALKIEKELGLEEGYLMILQIYHDIEKEKMNLNLSRPDLSKIRPVLF
                     WDTEPEKINWILQKEAVIKRVFERGNEEEKDELIRFYGIDAVNEVIKNGNS"
     gene            1166..1579
                     /locus_tag="PKM34_02415"
     CDS             1166..1579
                     /locus_tag="PKM34_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ82466.1"
                     /translation="MASKTSKVIILNKRDFKAKIFDYNYSKEWEYKGDMPAVIDFYAD
                     WCQPCKVVSPLLDQLSMEYDGQVKFYKINTEKDPEVSKVFGISSIPTLLFIAREGRPV
                     VVRGAQPIGALRKNIERILPGEKKNFLQGLFSFKK"
     gene            1704..2096
                     /locus_tag="PKM34_02420"
     CDS             1704..2096
                     /locus_tag="PKM34_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82467.1"
                     /translation="MRKLSGIILTMALTAFFAVQVMAQTATPAATDDKPVKQTATTAQ
                     TCGKFVDNNNDGVCDNRGTQCKQGKGTGFTDANGDGICDHRSDGNCCKGNPDCCKGQK
                     SGCGKGYGPKHRQGCGGPCGARTVPDKK"
     gene            2189..2785
                     /locus_tag="PKM34_02425"
     CDS             2189..2785
                     /locus_tag="PKM34_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ82468.1"
                     /translation="MTEQELISKANGRDREALSQLVETYQRKIIKTAYYYLGNMEDAE
                     DLSQEIFVKILTSLPRFRQSSSLSTWIYRIAVNESLNAVRRNRQRQIFCRIERIFGIT
                     ENGSKGIRREIQAETDTLEAEYQRQWLWEAISSLPEKQRTVFILHKMEELPYAEISSI
                     TGQTLSSVESLMHRARLNLQKKLLKPDITNIKTTSHEL"
     gene            2775..3203
                     /locus_tag="PKM34_02430"
     CDS             2775..3203
                     /locus_tag="PKM34_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82469.1"
                     /translation="MNCKEFRSFLILSQDQALNPKQLPGMEDHARDCRSCKMLLHKQE
                     LLEHFITAEQVREPNPFVSTRILQRLTGMQAQVTAGKSLVIRPVLVTLGLLAALAAGF
                     LIGTAGSVQEPGLTEENRIETLRTGLFVSDFADEDINLIE"
     gene            3215..3721
                     /locus_tag="PKM34_02435"
     CDS             3215..3721
                     /locus_tag="PKM34_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNQ82470.1"
                     /translation="METITRNRVLFWLLIFLIIVNLAALATYFILPEKKQPAVCAGDP
                     NSPECILHSRLNLTDEQNEHVERINNEYRVVSQPLSQEIKKLRGIILDELETGTPDTA
                     LIHNLTGEIAALQLQLHRENILHYLDLKEVCNPEQALLLSNLYREMYGCPMHREMGQP
                     KHHRYRGH"
     gene            complement(3774..5312)
                     /locus_tag="PKM34_02440"
     CDS             complement(3774..5312)
                     /locus_tag="PKM34_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ82471.1"
                     /translation="MRKEVLERLGISAINDGVCTGYDWHGGGGAVSVSVSPIDNKEIG
                     KVRNASPEDYEFVMQKAQNAFLEWRNVPAPKRGEVVRQIGDALRASKEDLAYLVTLEM
                     GKIYQEGLGEVQEMIDICDFAVGQARLLNGFTMHSERKDHRMYDQYHPLGIVGLITSF
                     NFPVAVWAWNSMIAAVAGDVVVWKPSSKTPLTAIATQNIIREVLKKNNVPEGVFNLLV
                     AGSNVLGDNFVADRRIPLFSVTGSTAVGKRVGEIVGKRLGRTILELGGNNAVIVSHSA
                     NLEMAIKSIVFGAVGTAGQRCTSTRRVIIHEAVYDAVKDKLVLAYNSVLKKIGDPLDK
                     ETLMGPLIDGKAVGNFLHALDQAKAEGGKVIFGGKVLEGEKYVSGNYVMPALVEAENH
                     YKIVQEETFAPILYLIKYRWIDEAIALNNGVPQGLSSAIFTENLREAEMFLSEAGSDC
                     GIANVNIGTSGAEIGGAFGGEKDTGGGRESGSDSWKLYMRRQTNTINYSTELPLAQGI
                     EFGF"
     gene            complement(5361..6989)
                     /gene="pruA"
                     /locus_tag="PKM34_02445"
     CDS             complement(5361..6989)
                     /gene="pruA"
                     /locus_tag="PKM34_02445"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNQ82472.1"
                     /translation="MNNALYNFPAPQNEPVSGYLSNSPERIALEQELHRQSLEKVEIP
                     LIIGGKEIRTGNTAELRMPHDHSKVIGIYHMVGEKEIKVAIEAALKAHQTWSNLAWTI
                     RASILLKAAELIATKYRALMNAATMLGQSKNIFQAEIDSVCETVDFLKYNSYFAGRIY
                     EIQPRSSFNQLNRMEYRALEGFILTISPFNFTSIASNLNMAPVMMGNTTLWKPATTSL
                     LSNYYLMKIFLEAGLPEGVINFIPGSGSLIGKTVLDSPEMAGIHFTGSNNTFNLLWGQ
                     VSANLASYRSYPRLVGETGGKDFIFVHPSADPLEVAVNAIRGAFEYQGQKCSAASRMY
                     VPQSLWPEIKPMLIDLANDIKIGDVRDAGNFMNAVIDKGSFETIKGYIDFAKVSAEAK
                     ILAGGDCDDSVGYFIRPTLVEVSDPKHKLMAEEIFGPVLTIFVYKDADLDETIELCNT
                     TSPYGLTGAVFSRDRVAASRICEKLQYAAGNFYINDKPTGAIVGLQPFGGSRASGTND
                     KAGGEFNLVRWISPRTIKETFVPATNYKYPYMME"
     gene            7171..>8383
                     /locus_tag="PKM34_02450"
     CDS             7171..>8383
                     /locus_tag="PKM34_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ82473.1"
                     /translation="MEDRRRIIIYLPVILAFVLILGIWLGSKMNFSKSGETQNPIFNR
                     AVQHDKITDLINYVEQDYVDSVSREDLTVNAIEGVLDKLDPHSTYIPAEDFGEAEDQL
                     DDNFEGIGIQFRIEQDTIMVIQVIPGGPSEKVGLMAGDRIVMINDSLVAGTGLTSRDA
                     VKQLKGKRGTVVKVGILRRGLAELVDFSITRDVIPTYSIDISYMVNDSVGYVKLSRFA
                     VSTHEEMIRAMSGLLENGMKRLILDLRDNTGGYLQASIHVSDEFLGDKKLIVYTEGNS
                     RPNQYAYATKRGVFEDYPLVILINEASASASEIVAGAIQDNDRGLIVGRRSFGKGLVQ
                     EQLTFPDGSAVRLTVARYHTPTGRSIQRPYKEGEKDEYYHELYDRYAHGEMEHPDSIS
                     LTDTVKYYTPAG"
BASE COUNT         2169 a         2117 c         1969 g         2128 t
ORIGIN      
        1 aagaacagga gtcatcagct cgtgaggcgg ttcagggcga actccatcgc cagcggcaaa
       61 aatacttatt taaaccattc aatcaaatcc agcttgatta attcccagtg tttgcctttc
      121 aggcagaacg gattaaagtc atcatcagcg ctgttgaaat cagtcagctt ttttaatatt
      181 tgggacggat catggctatg cggatacctt ttctcatgta aatcaagcat ttgttgaatg
      241 ctgaagtgat tcaacaattc atggatatcc cagaaatcct tttttctacc gcctccggat
      301 ataacatcta atttcattgc tgatatctca ttcatgcctg aaagtcgatg aaatcgtaat
      361 ccctatctgt aaacaggtct atatctaatg atttccggtg tccaagctgc aaacttaatg
      421 cggttcctcc gaccagcctg aagggcctga actcatcgct taccatcaaa gccttcagga
      481 tttcaagcag aagaggtgtt gtagtattat agaacaattt atgaattgcc attctttata
      541 acttcattga cagcatcaat cccgtaaaaa cgaattaatt catctttttc ttcctcattt
      601 cctctttcaa aaacccgttt aattaccgct tctttctgca aaatccagtt aattttttcc
      661 ggttctgtat cccaaaacaa aaccggcctt atttttgaaa gatcggggcg tgatagattc
      721 aggttcattt tttctttttc aatgtcatga taaatctgaa gaatcattaa atagccttct
      781 tcaaggccta gttccttttc aattttcaat gccagagaag tattcatttt acgttttcct
      841 ttggtgatgg aaaccaaagt ctgtggatat tccccgacgg aaattgcaaa tttgctctta
      901 cccagatttc gccgtctgag ttcacgttca aggtaaaatc ccggatggat acctttaata
      961 attattaatt cattattcat cctgcagaaa taaacataat aatgtaagca taaacgttta
     1021 tttattcaat gattgatcac ccctggtatt ttctatttcg caaaactatt attagtgata
     1081 ttaaagcgat ttatttctgc ttttttatta cttttgccac caaagcacgg aagcacgaaa
     1141 ttctattatc ttaaatcatg ataacatggc ttcaaaaacc agtaaagtaa tcatcctcaa
     1201 caaaagggat tttaaagcga aaattttcga ttataactat tccaaggaat gggaatacaa
     1261 gggggatatg ccggccgtaa tcgactttta tgccgactgg tgccagccat gcaaggtggt
     1321 ttcccctctg ctcgaccagc tttcgatgga atacgacgga caggtgaagt tttacaagat
     1381 caacaccgaa aaagatccgg aagtctccaa agtgttcggc atttccagta tcccaacatt
     1441 gttgttcatt gcccgtgaag ggaggccggt ggtggtgagg ggagcacaac ccatcggggc
     1501 gctgcggaaa aatattgaaa ggattctgcc gggcgaaaaa aagaacttct tacagggttt
     1561 attttccttt aaaaagtaag gctttatgca agcatccggc ggttgattat atgcatccct
     1621 gacgcattga attaaaaatt ttcatacgac cgcaagtttt ttgattttag gttgtctaaa
     1681 aggaaaaatc aaattttaaa agtatgagaa aattatcagg aattatttta acgatggcgc
     1741 tgaccgcctt ttttgcagtc caggtaatgg cccagacggc tacgcctgcc gccactgacg
     1801 acaagccggt gaagcaaacc gccaccacgg cccaaacctg cgggaagttt gttgataaca
     1861 acaacgacgg agtctgcgat aaccgcggta cccagtgcaa acaaggcaag ggaacaggat
     1921 ttaccgatgc caacggcgac ggcatttgcg accaccgcag cgatggaaat tgctgcaaag
     1981 ggaacccgga ttgctgcaaa ggccagaaga gcggatgcgg caaagggtat ggccctaaac
     2041 accgccaggg ttgcggcgga ccttgcggag cccggaccgt gccggacaaa aaataaagcg
     2101 taattttatc gaaccgtcgg gtgcccggtt gtcaataatg gataaccggg cgcctgaatt
     2161 gttgaggtgt attctaactg aacttcggat gaccgaacag gaattgatca gcaaagcgaa
     2221 tggccgcgac cgcgaagccc tgtcacaact ggtcgaaacc taccaaagaa aaattatcaa
     2281 gactgcgtac tattaccttg gcaacatgga ggatgcggag gacctgtcgc aggaaatctt
     2341 tgtaaaaatc cttacttcgc taccccggtt ccgccaatca tcgtcacttt ctacctggat
     2401 ttaccggatc gctgtgaatg aatcattaaa tgccgtcagg agaaacaggc aaagacagat
     2461 tttctgcaga attgaaagga tattcgggat cactgaaaat ggaagcaaag gcataagacg
     2521 ggagatccag gcagaaactg acaccttaga ggctgaatat cagagacaat ggttatggga
     2581 agccatttct tctttaccgg aaaaacaacg gactgttttt attttacata aaatggaaga
     2641 attgccttat gcagaaatat cctctattac cggccagacc ctgtcgtcgg tcgaatcgct
     2701 gatgcaccgg gccaggctga atctccaaaa aaaactgctg aagccggata tcacaaacat
     2761 taaaactaca agccatgaac tgtaaggaat ttcggagttt cctgattcta tctcaggacc
     2821 aggcgctcaa tccgaagcaa ctgcccggca tggaagacca tgcccgggat tgccgatcct
     2881 gcaaaatgct gctgcataag caggaactcc tggaacattt tatcacagct gagcaagtcc
     2941 gggagcccaa tccctttgta tcgacacgga tcttgcaaag gctgaccgga atgcaggcgc
     3001 aggtcacagc cgggaaatca ctggtgatca ggcccgttct ggttaccctt gggctattgg
     3061 ccgccctggc cgccggattc ctgatcggta ccgccgggtc agtccaggaa ccgggcttaa
     3121 cggaagagaa ccggatcgaa acactgcgta caggcctatt tgtcagcgac tttgccgacg
     3181 aagacatcaa tctgatcgaa taaaatccaa tcctatggaa accataacac gaaaccgcgt
     3241 acttttctgg ctgctcatct ttttgatcat agtcaacctg gcagccctgg caacttactt
     3301 tattttgcct gagaaaaagc aacctgccgt atgcgccggc gatcccaatt cacccgaatg
     3361 tatcctgcat tccaggctca atcttaccga tgagcagaat gagcatgttg aaaggataaa
     3421 caacgaatac cgggttgttt cgcagcctct gtcacaggaa atcaagaaat tacggggcat
     3481 catcctggat gaacttgaaa ccggaacgcc cgatacagcc cttatccata atctgaccgg
     3541 tgagattgcg gcgcttcagc tacagcttca tcgtgagaat atccttcatt atcttgatct
     3601 gaaagaggtc tgcaatccgg aacaagccct gctcctctcc aatctttacc gggagatgta
     3661 cggttgcccc atgcaccggg aaatgggcca accaaagcat caccggtaca ggggacattg
     3721 aatgtgagaa gggttcccgg ggttgcaaat ccacccttgc accccttacc acctcagaat
     3781 ccaaactcaa tcccctgagc cagaggcagt tcggtactgt aattgatcgt attggtctgt
     3841 cttctcatgt aaagtttcca gctatcggag cccgattcgc gcccgccgcc ggtatctttc
     3901 tcgccgccga atgcgccgcc gatctcagcg cccgaggtgc cgatgttcac attggcgatg
     3961 ccgcaatcgc tgccggcttc ggagaggaac atctccgcct cgcgtaagtt ctcggtgaag
     4021 atggcagagg atagcccctg cggaacgccg ttgttaagcg caattgcctc atcaatccaa
     4081 cggtatttga tcaggtaaag gatcggggca aaggtctcct cctgaacgat cttataatgg
     4141 ttctccgcct cgaccagggc cggcatgacg taattcccgg atacatactt ttcgccttcc
     4201 agcacttttc ctccgaaaat tacctttccg ccctcggctt tggcttgatc aagcgcatgc
     4261 aggaagtttc cgactgcttt tccgtcgatc agcgggccca tcaacgtctc tttatccagc
     4321 ggatccccga tctttttgag gacgctgttg taggccagca ccagcttgtc tttcaccgca
     4381 tcgtatacgg cttcgtggat gatcaccctg cgggtggagg tgcagcgctg tccggccgtt
     4441 cctacggcgc caaaaacgat cgacttgatc gccatctcca ggttggccga atgtgatacg
     4501 atcactgcgt tgttgccgcc gagctcgagg attgtccggc ccaggcgttt gcccacgatc
     4561 tcaccgactc tttttccaac agcagtcgat ccggtcaccg aaaacagggg gattcgcctg
     4621 tcggccacga aattatcgcc caggacattc gacccggcaa ccagcaggtt gaagacacct
     4681 tcggggacat tgtttttctt cagcacctcc cggatgatat tttgtgtggc gatggccgtt
     4741 aacggggttt ttgagctcgg tttccagacg accacatcgc cggcaacggc agcgatcatc
     4801 gaattccagg cccatacggc cacagggaag ttaaagctgg tgatgagtcc gacaataccg
     4861 agcgggtggt actggtcgta catccggtgg tctttccgtt cggagtgcat cgtgaaaccg
     4921 ttcagcaaac gggcctgccc cacggcaaaa tcgcagatat cgatcatctc ctgcacttcg
     4981 ccaagccctt cctggtatat tttacccatt tccagcgtaa ccaggtaggc caggtcctct
     5041 ttggaggccc tcagtgcatc cccgatctgg cgcaccacct caccgcgttt tggcgccggc
     5101 acgtttcgcc actccaggaa ggcattctgt gctttttgca tcacaaattc ataatcttcc
     5161 ggtgaagcat tccttacttt gcctatctct ttattatcaa tcggggaaac agaaacggag
     5221 accgctcccc cgcctccatg ccagtcataa cccgtgcata cgccatcgtt tatggctgaa
     5281 attcccaaac gctcaagtac ttcttttctc atgttcattt tattaaatct tgataataaa
     5341 cccttctgaa ggaaaattgt ttattccatc atataaggat atttgtaatt tgtggctggt
     5401 acaaaggtct ccttgatcgt tcgcggactg atccaccgga cgagattaaa ctcgccgccg
     5461 gctttatcat tggtccccga agcccttgat ccgccgaaag gctgaagacc tacaatggcc
     5521 ccggtaggct tgtcgttgat gtagaaatta ccggcggcat actgaagctt ctcgcaaatc
     5581 ctcgatgcgg ccacacggtc gcgggaaaac acggctcccg tcagcccgta aggagaggtg
     5641 gtgttgcaca attcaatagt ttcatccaga tcggcatcct tataaacaaa aatagtcagt
     5701 accgggccga agatctcttc tgccatcagt ttgtgcttcg gatctgaaac ctcaacaagg
     5761 gtcggacgaa taaaatagcc caccgaatca tcgcagtcac cgccggcaag gatctttgct
     5821 tcggccgata cttttgcaaa gtcgatatat cctttgatcg tttcgaatga gcctttgtcg
     5881 atcacggcat tcataaagtt accggcatcc ctgacatcgc caattttaat atcgttggcc
     5941 aggtcgatga gcatcggctt gatctcaggc cacaggcttt gcggcacata catcctggag
     6001 gcagccgagc atttttgtcc ctggtattca aaagcacccc ggatggcatt gacggctact
     6061 tccagcggat cggcggatgg atgaacgaaa atgaaatctt tgccaccggt ctccccgacc
     6121 agcctggggt aggatctgta gctggcaaga ttggccgaaa cctgccccca gagcagattg
     6181 aacgtattat tggaaccggt aaaatgaatc cctgccattt cgggcgagtc caggaccgtt
     6241 tttccgatca ggcttccact gccgggaatg aagttgatga caccttccgg caaaccggcc
     6301 tcgaggaata tcttcatcag gtaataattg gagaggagcg aagtggtcgc cggtttccag
     6361 agagtcgtat tgcccatcat aactggcgcc atgttcagat tggaggcaat ggaagtaaag
     6421 ttgaacgggc tgatggtcag gatgaaccct tccagtgccc ggtattccat ccggtttaac
     6481 tggttaaagc tcgaccgggg ttgaatttcg tagatccggc cggcaaaata ggagttgtat
     6541 ttcagaaaat cgacagtttc acatacactg tcgatttcag cctggaagat attcttgctc
     6601 tggccgagca tagtggcagc attcataagc gcacggtatt ttgtcgcaat tagttcggcg
     6661 gccttgagca ggatggaggc ccggatggtc catgccaggt tcgaccaggt ctggtgtgct
     6721 ttcagagcgg cttcaatggc cactttaatc tctttttctc caaccatgtg gtatattccg
     6781 atgacctttg aatggtcatg cggcatgcgt agctcagccg tgtttcccgt cctgatctct
     6841 tttcctccga taattaaagg gatttccacc ttttccaatg attggcggtg aagttcctgt
     6901 tccagggcaa tcctttcagg actgttgctc aaatacccgg ataccggctc gttctgaggt
     6961 gccgggaagt tgtatagtgc attattcata gcatctgttt ttaataaaaa cgttatgcac
     7021 atcattgcat aacggtcagc aaaaataaaa aaaaccggac aattattaaa ctcatgcaat
     7081 aaccccctcc ccccttacca ccctttgagg ttcctgatga cgtgctgccg actaattttc
     7141 ctatctttgc aaacttcaag aaaatcagct atggaagacc gtcgtcggat catcatatac
     7201 cttcctgtta ttctcgcttt tgtgcttatc ctgggcatct ggctcggcag caagatgaac
     7261 ttcagcaaga gcggtgaaac acagaatccc atatttaacc gggctgtcca gcacgataag
     7321 atcacggacc ttatcaatta tgtcgagcag gactatgtcg attctgtcag ccgggaggat
     7381 ctgaccgtga atgctattga gggagttctg gacaagctgg atccccactc cacttatatc
     7441 cctgctgaag attttggcga ggccgaagat cagcttgacg acaatttcga agggattggc
     7501 atccagttcc ggatagaaca agacaccatc atggtcatcc aggtcattcc gggaggcccc
     7561 tcggagaagg tcggcctgat ggcaggcgac aggattgtca tgatcaacga ttcgctggtt
     7621 gcgggtaccg ggctgaccag cagggacgcg gtcaaacagc tcaaaggcaa gcgcggcacc
     7681 gttgttaaag tgggcattct tcgccgcggc ctggccgaac tggttgattt cagcatcacc
     7741 cgcgatgtta ttcccaccta cagcattgat atttcctaca tggtcaacga ttcggtcggt
     7801 tacgtcaaac tcagccggtt cgccgtcagt acacacgaag agatgatccg ggccatgagt
     7861 ggtctcctcg aaaatggaat gaaacggctt atccttgatc tccgcgataa taccggtggc
     7921 tatctccagg cttcgatcca tgtgtcggat gagttcctgg gcgataaaaa gctgatcgtt
     7981 tacaccgaag gaaacagtcg tccgaaccag tatgcatatg cgacaaaaag gggtgtcttt
     8041 gaagattatc cgctggttat cctgatcaat gaagcatcgg cctcggccag cgaaatcgta
     8101 gccggcgcca tccaggacaa tgaccgggga ctgatcgtcg gacgcaggtc gtttggcaaa
     8161 gggttggtac aggagcaact tactttcccc gacggatcag ccgtaaggct taccgttgcc
     8221 cgctatcata ccccgacggg acgcagtatc cagcggcctt acaaggaagg tgaaaaggat
     8281 gaatattacc atgaactcta cgaccggtat gcccatgggg agatggaaca tcccgacagc
     8341 atttctttaa cagatacggt taaatactac actcctgccg gga
//
LOCUS       DAOQXS010000048         8354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_8999_length_8354_cov_4.038077, whole genome shotgun sequence.
ACCESSION   DAOQXS010000048 DAOQXS010000000
VERSION     DAOQXS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_8354_cov_4.038077"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKM34_02455"
     CDS             <1..191
                     /locus_tag="PKM34_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82474.1"
                     /translation="LKVDIFGMSSEDWISLLQNLKDGKIEMADMLALASSMSTHGRKS
                     IKFVPIWMNAICSNMKQV"
     gene            152..934
                     /locus_tag="PKM34_02460"
     CDS             152..934
                     /locus_tag="PKM34_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82475.1"
                     /translation="MDERHLQQYEASINKEKEMLDKSLKYKRISQERYNAKVTALDKK
                     LDQEKRKIAREQASRAKTQAIFSAVINTASAVISALATPPPWLGIVLAALVGGMGALQ
                     IATIASEPVPQFAKGKYDVIGEQDGKKYSAPVLGSPSTGLINSPAILVGEKPEIIIDP
                     ATTKNLMVNYPAVIEAIHSARIPQFATGDYSGINSFEGTKGSQKLIAGVLAAQTASIN
                     RLNRNLEKGIQAKLLADDEFVRTSKDVNSRYDKLASKANSSL"
     gene            978..2582
                     /locus_tag="PKM34_02465"
     CDS             978..2582
                     /locus_tag="PKM34_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82476.1"
                     /translation="MALSVVKNLETICPVDNPKVVTIGTDNQFSTVGVKAVLSLLFTT
                     VDETLGHLIMLRWNGITKAFALAASPDNSGLHIHVGLPSYQLWDWVALLCEDLSNNYY
                     LSRDFDLVASGPYITITAKADGEEFTLEYLDDIEPVTGLSADPAPVSGVTPVLRDFYS
                     IILQVKPHNSDVLLGQDMVTPDVNGHSSFDIHDLLCDYLTPEFAWPHEAGYTHVPRLK
                     FLKKFRVQYAETYDRHVYKLNTLPGDHYAVLGGFDYKILAALNGIAYSYLDFITTFKA
                     FLTWQPAIKPINKTQREKLFYLVFNDISNVVSFIKIYFTDGTTSSNTQIASFHAAQYD
                     VIEFFTDYISLDIAGKEGQKTALKYDFWLQDESNNIISQVRTYEIEFRRQPFSRILIF
                     RNSFGAYDIFRATGRKKHVNNYERMILERNSSEYTLAQQYQILESESFQVSSGFISRS
                     MKNWLRELMLSEEVFEIIGDYRFPILIQNEQVENIDDQETIYEVDIKYKYAFKNPKYS
                     ELSNTMPLLAENLNALLNEDGNFLFA"
     gene            2554..3756
                     /locus_tag="PKM34_02470"
     CDS             2554..3756
                     /locus_tag="PKM34_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82477.1"
                     /translation="MKTGTFYLRNLHRILLPFLFLLLLSPAFPQRISELPAATYIGIG
                     DLMPIVQNGVTKKLPFAGLTGSLSFLKDSLFVKYRCHKIKGASQWYFSGDTIYIDTCY
                     ISIPPGLGRTDSLFFSYGCHSGETKWYYSSDTIYLDTCFAAVAGDTCHWYLNQDTLIN
                     KNGYKILLTDSVYFSNYAIPHLEEGEKFMGFLTLKSYLGNVKQLAYATPELAADSLAV
                     NFDTLSCYQQIGDTNQYDATKNDLHDSIQMAIDSVKNWVEDQNYVSGITSSGYSMQFG
                     SASISSSDGVTLYFMSSNYSSTSTVARVYVPKTGTIKTCYFWIWLGSPGSCSNEPMTC
                     YIRLNSTTDYAIATVSTTDGGKQFVNTDLSIPVSQGDFLSIKSVNPIWQTNPSGGMNG
                     CTGIIFIE"
     gene            3776..4084
                     /locus_tag="PKM34_02475"
     CDS             3776..4084
                     /locus_tag="PKM34_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82478.1"
                     /translation="MTVQDILTIIGLFLLYATGLIGVYVNLRIKLKELEIKLFNLQDE
                     FAQHKLLIGNNVTKLEERNTIEHDSISVKIDKLLEKITEIRVDQASNNVINKSKPKQS
                     "
     gene            4081..4296
                     /locus_tag="PKM34_02480"
     CDS             4081..4296
                     /locus_tag="PKM34_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82479.1"
                     /translation="MKTGYFEEAPGVKSAMRLQSFISLWIGGGIACFAIGTRQLDVNV
                     IAVITLFIVAAFVPKAVQKFAEIKSTT"
     gene            4334..4942
                     /locus_tag="PKM34_02485"
     CDS             4334..4942
                     /locus_tag="PKM34_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82480.1"
                     /translation="MTKYFLIVIAILLFALAGLTKIWFVTLKDRNRISDNQSALLSEI
                     QYFKLNDSTNAATIGSLTLKLSEIKQASNTQFDAMKATLKLLDIKLRNLDSYSSSEFE
                     NLLTINTFLRDSVLLDTLPVKYLYAKSKWNEVTVSVFPERQDSISLKVITYDKMEQVL
                     WKEGRGLKFWKKSFWRKRPIHQTIRFQNPDTKITYPFSVIVK"
     gene            5088..7229
                     /locus_tag="PKM34_02490"
     CDS             5088..7229
                     /locus_tag="PKM34_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82481.1"
                     /translation="MFSIVVGGQQVDHSGITLNLYLKSTFPFTPDQGQIEGSFAFNGS
                     LPNSPLNKRIFGFPHRLEGVFDLPLNIPCKLTINARVWYDALISITSATQVSLNFDLL
                     IGYGSFASLIEGKSLKDLTFPDLIDMGEDQAAVIAFANEIAGQSYPVTSFNFPMIYAP
                     DFYGDKNPTYLDFINLYVADSYQQNVININGAQNFNALVPMLYLQYVARLCFESFGYK
                     PSGTFFDDPDIAKAMLYNNYAIDQKYKKFLVQAYQGAAQEFPPMPTKIMFTDDSTFPN
                     VDEYDIFNTATSEYTISAVGEYMIPLSFKFYNLYDDPMKLKVSLYCDSELVETVTFNE
                     TVWWQWVSREVFFTKYFDANSIGKKLSVSINLEDLYGGNICNAYIKQASIVFENISLT
                     DLNEFSHQIDLRNHVPDKEVSAFLINLQLIFGVIIGFDHEHKLADVSFVRDALLFNEG
                     HDLTEFTRLSSKKVTIVPPEGYKIRFEFDSSDEETNMVSDSVDLSNYIGTFSTFSSLP
                     FPSKLGDVAFVSNTNCYYIFSKIETGASVWDLLCHYHPVITVGEGLNEVKPDMSPILM
                     HRHPGTRTILPVIRQQGSSLAFSDEINETELRFFFYHGLCMQAEFDNLYPFASSLSYD
                     PRGNAIGNLDLTFTGSRGVYELYLKNYYYWLTNLMKQAVFSTRMTFSLLKTLDLQKIY
                     RIFNSRFLIKEAKVAVSEDEMADVEIECWKV"
     gene            complement(7289..7969)
                     /locus_tag="PKM34_02495"
     CDS             complement(7289..7969)
                     /locus_tag="PKM34_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82482.1"
                     /translation="MKKIIITHYSSPDKTSLRTADRKYRVVTGFDNHTFLFETKREVE
                     DFLRKLSQDMTDLLFLYNESFSRAFTAYRSLWFYMDAAEQSEINVNHDCDNLTVSLTR
                     LFDRTVHWDHGGDAWRLTQDLRLIGNELKAFWGLLVKVTAKRNQWADNYRFKLEIDMV
                     DNFRDRMESIVQPISDKEPVVTFVEMVTAKEILDHPVNLNSDYEKKKFASSLIRLRKQ
                     TKKEVINE"
     gene            complement(8156..>8354)
                     /locus_tag="PKM34_02500"
     CDS             complement(8156..>8354)
                     /locus_tag="PKM34_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82483.1"
                     /translation="YDTPQDVYDAEKAKAKEIYLKSVPYLEKSFELEPGDMDAANSLR
                     AVYGVLEMADKLKALNESINK"
BASE COUNT         2305 a         1871 c         1693 g         2485 t
ORIGIN      
        1 cgttgaaggt tgacattttt ggaatgtcaa gcgaagactg gatctccttg ctgcaaaatt
       61 tgaaggatgg gaaaatcgaa atggctgaca tgcttgcact ggcttcatca atgtcaacgc
      121 atgggcgcaa atcaatcaaa ttcgtgccaa tatggatgaa cgccatttgc agcaatatga
      181 agcaagtata aataaggaaa aagaaatgct ggataaatct ttgaaataca aaaggatttc
      241 ccaggaaagg tataatgcca aagttactgc actggataaa aagcttgacc aggaaaaaag
      301 aaagattgcc cgtgaacagg cttcccgtgc taaaactcaa gctattttct ctgcggtcat
      361 aaataccgct tccgccgtta tttctgccct ggcaaccccg ccaccctggt tgggtatcgt
      421 gcttgcagcc ctggtaggag ggatgggcgc tttgcaaatt gccaccattg cttcagaacc
      481 tgtaccacag tttgccaaag ggaagtacga tgtcatcggc gaacaggacg ggaaaaagta
      541 ttccgcgcct gtactgggtt caccatcaac cggcctgatc aactccccgg ctatcctggt
      601 aggggagaag ccggaaatca tcatcgatcc ggccaccact aaaaacctga tggtgaatta
      661 ccctgctgtc atcgaagcca tccattcggc caggattccg caatttgcca caggcgatta
      721 ttccggtatc aattcctttg aaggcaccaa aggaagccag aagttgattg ccggagtatt
      781 ggcagcgcaa accgcttcca ttaaccggct taaccgtaat ctagaaaaag gtatccaggc
      841 taagctttta gctgatgatg aatttgtccg taccagcaag gacgtgaatt cacgctatga
      901 caaattagct tctaaagcca attcctcact ctaatcttgt cctttccctt ttgcccgttc
      961 cttgacaaat ttgcggcatg gctttatccg ttgtaaaaaa cctcgaaact atttgtccgg
     1021 ttgacaaccc taaagttgtc accatcggaa cggataacca gttttccact gtaggtgtga
     1081 aagctgttct tagtcttctt tttactactg tcgatgaaac tttagggcat cttatcatgc
     1141 ttcgctggaa tggcattacc aaagcttttg ccctggctgc ttcgcccgat aattcaggtt
     1201 tgcatattca tgtgggccta ccgtcatatc agctatggga ttgggtagcc ttactttgcg
     1261 aggacctgtc caataactat tatctctcca gggactttga cctggtcgct tccggccctt
     1321 acattaccat cacggcaaaa gctgacggag aggaatttac cctggaatac ctggatgaca
     1381 ttgaaccggt aaccggttta tcggctgatc ctgcgccggt gtccggtgta acgccggttc
     1441 tcagagactt ttacagtatc attcttcagg ttaaaccgca taattctgat gtgctcttag
     1501 ggcaggacat ggttacccct gatgtaaacg gacattccag ttttgatatc catgatctgc
     1561 tgtgtgatta ccttactcct gaattcgcct ggccacatga agccggttac acgcatgttc
     1621 cccggttaaa gtttctcaag aaattcaggg tccagtatgc ggaaacctat gacaggcatg
     1681 tgtataaact caatacgctg cccggtgatc attatgcggt cctgggaggt tttgattaca
     1741 aaattcttgc ggcccttaat ggtattgctt acagttattt ggatttcata accaccttca
     1801 aagcctttct gacctggcaa cctgccataa aacccatcaa taagacgcag cgggaaaagc
     1861 ttttctacct ggtgttcaat gatatttcga acgtggtttc tttcatcaag atttatttca
     1921 ccgatgggac cacttcgagt aatactcaaa ttgcatcgtt ccatgctgct caatatgatg
     1981 tcattgaatt cttcaccgat tacatctctc ttgatattgc tggcaaggaa ggtcagaaga
     2041 ccgcattgaa atatgacttc tggcttcagg atgaatccaa caatatcatt tcccaggtca
     2101 gaacctatga aatcgaattt cgccggcagc cgttctcccg gattttgatc tttcgtaatt
     2161 cctttggcgc ttacgatatt ttccgggcga ccggccggaa aaagcatgtc aataattacg
     2221 agcggatgat tcttgagcgc aattcttctg aatacacgct ggcccagcaa taccagatcc
     2281 ttgaatctga atccttccag gtcagttcag gatttatctc caggtcaatg aaaaactggc
     2341 ttcgggaact gatgctgtca gaagaagttt tcgagatcat cggggattat agattcccga
     2401 tcctcattca aaatgagcag gtcgaaaata ttgatgatca ggagactatt tatgaagtcg
     2461 acatcaaata caagtatgcg tttaagaatc cgaaatattc agaattgtcg aataccatgc
     2521 cgcttttggc tgaaaacctt aatgctttgc tcaatgaaga cgggaacttt ttatttgcgt
     2581 aatctccata ggatattact gccttttctt tttctcctgc tgctttcacc ggcatttccg
     2641 caacgtattt cggaactgcc tgcagctacc tacataggca tcggggatct catgcccatc
     2701 gttcaaaacg gagtcactaa aaagcttcct ttcgccggat tgaccggctc acttagtttc
     2761 ctgaaagatt cccttttcgt caaatatcgc tgccataaga taaagggcgc aagtcaatgg
     2821 tatttctcag gtgataccat ctacattgac acctgctaca tctctattcc tccaggactt
     2881 gggagaacag attcattatt cttttcttat ggctgccata gcggagagac aaaatggtat
     2941 tattccagtg atacgatcta tcttgatacc tgctttgctg ccgtagctgg tgatacatgc
     3001 cactggtacc tgaatcaaga taccctgatc aataagaatg gctataagat cctgcttaca
     3061 gatagtgtct atttctcaaa ttacgccata ccgcatctgg aagaagggga aaaattcatg
     3121 ggatttctaa cgcttaaaag ctatttaggc aatgtgaaac aactggccta tgctacaccc
     3181 gaacttgcag ccgattcgct ggcagtcaat tttgatacgc tttcctgtta tcagcagatt
     3241 ggagatacga accaatatga tgcaaccaaa aatgatctcc atgattcaat ccaaatggcc
     3301 attgattcag taaaaaattg ggttgaggat cagaattacg ttagcgggat tacttcatcg
     3361 ggttattcca tgcaatttgg ttcggcatcg atctcaagca gtgatggcgt cactttatat
     3421 tttatgtctt ccaattattc atcaaccagc accgttgcta gggtttatgt tcctaaaact
     3481 ggaaccatta aaacatgcta tttctggata tggcttggat cacccggttc atgctctaat
     3541 gagccaatga cctgctacat ccgcctgaat agtacaactg actatgccat agcaaccgtg
     3601 agtacaaccg atggaggtaa gcaatttgtc aacaccgatc tatcaattcc cgtttcacaa
     3661 ggagattttc tttcgatcaa atcagtcaat cccatctggc aaacaaatcc gtcaggtgga
     3721 atgaacggat gcacggggat tatcttcatc gaataaccct ctcaatgaca atagaatgac
     3781 agttcaagac attttgacaa tcatcggtct gttcctcttg tatgcaaccg gcttgatcgg
     3841 tgtttatgtc aatttacgca tcaagctgaa agagcttgaa ataaagctct ttaatctgca
     3901 ggatgaattt gcccagcata aactgctcat tggcaataat gtaacaaagc tggaagaacg
     3961 caataccatc gagcatgatt ccatcagcgt caaaattgat aaactactgg aaaagatcac
     4021 ggaaatccgt gtagatcagg cttcaaataa tgttataaat aaatcaaaac caaaacaatc
     4081 atgaaaacag gctatttcga agaagctccc ggcgtgaagt ccgccatgcg cttgcagtca
     4141 ttcatctctc tctggatcgg cggcggcatt gcctgcttcg ctatcggcac caggcaattg
     4201 gatgtaaatg ttattgcggt tatcacttta ttcatcgtgg ccgccttcgt tccaaaagcg
     4261 gtccagaaat tcgctgaaat caaatccaca acctgatatt aaagtatttt ctcaaatcaa
     4321 agcttcataa gtcatgacca aatatttctt aatcgttatc gcaatcttac tattcgcgct
     4381 ggccggcctg accaaaattt ggtttgtaac ccttaaagat aggaacagga tatctgataa
     4441 tcaatccgct ttgctgtcgg aaatccaata tttcaaactg aatgacagca caaatgccgc
     4501 aaccatagga tctcttacct taaagctttc agagataaaa caagcctcga atactcagtt
     4561 cgatgccatg aaagccaccc ttaagctatt ggatatcaag ttaagaaatc tcgattccta
     4621 ttcatccagt gagtttgaga accttttgac catcaatacg ttccttcgtg acagtgttct
     4681 gttggacacg ctcccggtga aatatcttta tgctaaaagc aaatggaatg aggtaacggt
     4741 atcagtattc cccgagcgcc aggactcaat atctttaaag gtgataactt atgacaaaat
     4801 ggaacaggtg ctttggaaag aggggagggg actgaaattt tggaaaaagt ccttttggag
     4861 aaaaagaccg attcatcaaa caatcaggtt tcaaaaccct gataccaaaa ttacataccc
     4921 attcagtgtt atagtcaaat agtaaggtat gaatcagttt ttctccgccg ttttctttaa
     4981 aatatttttc ttatgggcgt tacctttcag gtcaggctat ccgcttcaat ctttaattct
     5041 taaaggattt attcttctat ccttaacgct ttcagggagg atttcaaatg tttagcattg
     5101 tagtaggcgg ccagcaagtc gatcattccg gcataacgtt aaatctttac ttaaaatcga
     5161 cattcccatt caccccggat cagggccaga tcgaaggttc gtttgcgttt aatggttcgc
     5221 tccccaactc tccgttgaac aagcgcattt tcggctttcc gcacaggctt gaaggtgttt
     5281 tcgatcttcc attgaacatt ccctgcaagc tgacaatcaa tgcccgggta tggtatgatg
     5341 cacttatctc cattacttcc gccacgcagg tctcgctgaa tttcgacctg ctaatcggtt
     5401 acggtagctt tgcttcactt attgaaggta aatcgttaaa agacctgact tttcccgatt
     5461 tgattgatat gggtgaggac caggcggcag tcattgcctt tgccaatgaa atcgctggtc
     5521 aatcctatcc ggtgacttcc tttaattttc ccatgattta cgcccctgat ttttacgggg
     5581 ataagaatcc gacatacctt gatttcatca atctttacgt tgctgacagc tatcagcaga
     5641 acgtgatcaa catcaacggc gctcaaaact tcaatgcgct ggttcctatg ctttaccttc
     5701 aatatgtggc caggctttgt tttgaatcgt ttggttataa gccatccgga acattcttcg
     5761 atgatccgga tattgctaaa gcgatgcttt ataataatta tgctatcgat cagaaataca
     5821 agaagtttct tgttcaggct taccaggggg cagcccagga atttccgcca atgcccacta
     5881 aaatcatgtt caccgatgat tctacttttc cgaatgttga cgaatatgat attttcaata
     5941 ctgcaacatc ggagtatacc atcagtgcag tcggtgagta catgattccg ctgtcattca
     6001 agttttacaa cctgtatgat gatcccatga aactcaaagt cagcctgtat tgtgattcag
     6061 aactggttga aacggttact ttcaatgaaa cagtctggtg gcaatgggtt tccagagaag
     6121 tatttttcac gaaatacttt gatgctaatt ccattggcaa aaagctttcc gtttcaatca
     6181 atcttgagga tctgtatggc ggaaatatat gcaacgccta tatcaagcag gcctccattg
     6241 tctttgaaaa catcagcctg acggatttaa atgaattttc ccatcagatt gacctgagaa
     6301 atcatgtccc ggataaggaa gtctctgcct tccttattaa cctgcaattg attttcgggg
     6361 ttatcatcgg ttttgatcat gagcataagc tggccgatgt gagctttgtc cgtgatgcac
     6421 tgcttttcaa cgaaggacat gatctgaccg aattcaccag gctttcatcg aaaaaggtca
     6481 ctattgttcc cccggaaggt tataagatcc ggtttgaatt tgattcttct gatgaagaaa
     6541 caaacatggt ctcagatagt gttgaccttt caaactatat cggaactttt tcaacttttt
     6601 ccagccttcc ttttccttca aagcttggag atgtggcctt tgtttccaat accaattgct
     6661 attacatttt cagtaagatt gaaaccggcg cttcggtttg ggatttactg tgccattatc
     6721 atccggtaat tactgtagga gaggggttga acgaagtaaa accggacatg tcacctatat
     6781 taatgcaccg gcacccgggc acccggacta ttctgccggt tatccgccag cagggttcgt
     6841 ctctggcctt ttcggatgaa atcaatgaaa ccgaattgcg atttttcttt tatcatggtc
     6901 tctgcatgca agccgaattc gataatcttt atccgtttgc atcgtccttg agttatgatc
     6961 ccaggggtaa tgcaattgga aatcttgatc ttacatttac cgggagcagg ggcgtgtatg
     7021 agctttacct taaaaattat tattactggc tgactaacct gatgaagcag gccgttttca
     7081 gtacccgaat gaccttctct ttattgaaaa cactggacct ccagaagatt tacaggatct
     7141 ttaattcacg ctttttgata aaggaagcga aggtcgccgt ttcagaagat gaaatggcag
     7201 atgttgaaat agagtgctgg aaagtttgaa caattgtctt agtatattac tttaggtaag
     7261 tcccgttaaa atgctttaaa caagtatttt attcattgat tacttccttt ttagtctgct
     7321 ttctcagcct tatcaaagag ctggcaaact ttttcttttc ataatcgctg ttcaaattga
     7381 caggatgatc taagatttct tttgcagtca ccatttcaac aaaggtgaca acgggctctt
     7441 tatcggatat gggttgtaca atgctttcca ttctatccct gaaattatcc accatatcaa
     7501 tttccagctt aaagcggtaa ttgtcggccc attggttgcg ttttgcagta acctttacca
     7561 aaagacccca aaaggctttg agctcgttgc cgatcaagcg cagatcctgc gtcaatcgcc
     7621 aggcatcgcc accatgatcc caatgcaccg tgcgatcaaa tagcctggtc aggcttacgg
     7681 tcaggttatc acagtcatgg ttgacgttga tttcgctttg ctctgcagca tccatataaa
     7741 accaaagact tcggtaagcg gtgaaagctc tggaaaaact ttcgttatat aagaagagta
     7801 ggtcagtcat atcctgactt aatttcctta aaaaatcctc aacttctctt ttggtttcaa
     7861 ataaaaaagt atggttgtcg aaaccggtga cgacgcgata ttttctgtcg gcggttctta
     7921 aactggtttt gtccggggag gaataatggg tgatgataat ttttttcatg ggacggcaaa
     7981 tttacattat taaaaatcag aaacatttgc aaatgttttt gtttcttttc acaattttca
     8041 accgcctgat tcccagtaat acctgcttta gccaaattgg ctattttgtc tcataattaa
     8101 tattatgtta aatgacaatt cgcaaataaa gggggaactt actccccctt atctgttatt
     8161 tattgatcga ttcgttcagc gctttcagtt tatcagccat ctccaatacg ccataaacgg
     8221 cccgcaatga attagcggca tccatatccc ccggttcaag ttcaaatgac ttttccaggt
     8281 aaggaacaga tttcagatag atctcctttg ccttagcttt ctcagcatca taaacatcct
     8341 gtggcgtatc ataa
//
LOCUS       DAOQXS010000049         8331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9033_length_8331_cov_3.714717, whole genome shotgun sequence.
ACCESSION   DAOQXS010000049 DAOQXS010000000
VERSION     DAOQXS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9033_length_8331_cov_3.714717"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="PKM34_02505"
     CDS             complement(<1..1953)
                     /locus_tag="PKM34_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ82484.1"
                     /translation="MEDIPAITQVHEYPVKVKKSFRQWLSLLLLFLVLFPVCSHSQEI
                     NKQKSEKAVEKAWQYYSRQEWDKCKAELNAAFRYDSTNAEPYVMMAEVLMEQSEPGQA
                     VIYYKKALERNPERWESLCYVLANTLFSLKEYEESKVYYDTILKISGIDSKPTQAAQE
                     RLGQAITRSALRAAPVAFNPVNLGIRINTSGDEYINAISTDGQFIFFTRRIKNPKPVQ
                     GRNYSEDLYIAEIGNNAGDSAALLTYPPGKENDAGSFCLSPDGRMILFTACNRADSYG
                     SCDLYYSEKINGKWSVARNLGRNVNSESWDAQPSISPDGRKIYFASNRAGGWGNSDIW
                     VTERDSLGHWQLPVNLGRPVNSSEAEMVPVIHFDNQTLYFSSKSHPGMGGADLFMSSK
                     INQSWTKPVNLGYPLNTEADELAIIVNASGKYGFISSNTLEGSGGYDIYSFSLNNEVR
                     PVPVTYFKGMIYDSITRQPLKADFEMIDTENDTTVVIAQSDDANGEFLVCIPCNRNYA
                     LNVSREGYLFYSAHFPIKENHDAVNPVVKNIGLIPIAPGNTMILRNIFFEKDQYILSA
                     ESTAELTKLLLFLNENPSIRIEIGGHTDSDGPDEYNLELSRKRAEAVYIYLTGNGIST
                     DRLTFKGYGESQPISDNETWQGKAQNR"
     gene            complement(1908..2552)
                     /locus_tag="PKM34_02510"
     CDS             complement(1908..2552)
                     /locus_tag="PKM34_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNQ82485.1"
                     /translation="MNTPLSPDLRNLLEQGKLLPLMEDFYSIQGEGFNTGKAAYFIRL
                     GGCDMGCWWCDVKESWDARLHPLTDTDEMVYRVLQQPAKSVVVTGGEPVLYNLDYLCK
                     KLKENGIETFLETAGVRPLSGSWDWLCLSPKKGSLPLPEYFREAAELKVIIHQESDFR
                     WAELNAEKITGKCHLYLQPEWSRYNQILPSIVNYIKTHPQWKISLQSHKFMNIP"
     gene            complement(2559..3437)
                     /locus_tag="PKM34_02515"
     CDS             complement(2559..3437)
                     /locus_tag="PKM34_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNQ82486.1"
                     /translation="MKLLDGKLVSQQIIEELKIEVASLIDSGTDPHLAAILVGEDPAS
                     QTYVRNKEKACHEAGIISSVYRQSAGISENELLAMIDFINNDPEVHGLIVQLPLQGHI
                     NPEKIVQAIDPKKDVDGFHPVNIGRMVQGLPSYLPATPYGIIKLLEHYRVETEGKHCV
                     VLGRSNIVGTPVSILMSRKNYPGNCTVTLCHSKTKDLGKLAKEADILIAAIGQPGFVT
                     GEMVKKGAVVIDVGMHRIPSDTTQSGYRLTGDVNFEEVSKIASMITPVPGGVGLMTIV
                     SLLMNTVKAAKKEIYG"
     gene            complement(3484..4827)
                     /gene="ffh"
                     /locus_tag="PKM34_02520"
     CDS             complement(3484..4827)
                     /gene="ffh"
                     /locus_tag="PKM34_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNQ82487.1"
                     /translation="MFEGLSDKLERSFRILKGQGHITEINVAETLKEVRKALLDADVS
                     YKIAKDFTDQVKQEAMGQKILTSVSPSQLMVKIVHDKLAELMGSSQAELNTQGKPAII
                     LMSGLQGSGKTTFSAKLAHYLKSKKGRQVMMAACDIYRPAAIEQIKVLGEQIGVEVYS
                     EPDNRDPVQIARRAVQYAKDKNYNVVIIDTAGRLAIDEEMMKEIAAIKNGVQPNETLF
                     VVDAMTGQDAVNTAKAFNERLDFDGVVLTKLDGDTRGGAALTIRAVVTKPIKFVGIGE
                     KIDAIDVFYPSRMADRILGMGDIVSLVERAQEQFDEEEARKLQKKIAHDEFNFNDFMT
                     QIKQIKKMGNVKDLMSMIPGMGKALRDVDIDDNAFKGIEAIIYSMTPDERENPKLLNG
                     SRRKRIAIGSGNDIQEVNRLIKQFYETSKMMKMMSAGKGKNMMRAMQNMKSQGRK"
     gene            complement(4898..4970)
                     /locus_tag="PKM34_02525"
     tRNA            complement(4898..4970)
                     /locus_tag="PKM34_02525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4935..4937),aa:His,seq:gtg)
     gene            5076..6152
                     /locus_tag="PKM34_02530"
     CDS             5076..6152
                     /locus_tag="PKM34_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82488.1"
                     /translation="MVFEKLKNNKPAIASVSFLIMVFVVAMAGYLITPDPTPLANRQF
                     LELGAARPGFSVTMLKLSDTGFHEKSGLIRKWIYGQRTPYRYIPVKNFFREGGNIIAE
                     AYTGDESQGQELRIDTKGSSFDKFIIVKRFPLGTDQFGRDLLSRLIIGARISFAVGLV
                     SVVISLFLGVTIGAAGGYFGGRVDKFVVWLINVLWSVPTLLLVIAITFALGKGFWQIF
                     VAVGFTMWVEVARITRGQILSVREKEYIEAAEALGFSRFRIIIRHILPNIAGPIIVVA
                     AANFATAILVEAGLSFLGIGVQPPMPSWGNMIRENYHFLLLGEYHLALFPGLVLMLTV
                     LSCMILGNGIRDAMDVRSSGTMVS"
     gene            complement(6166..7638)
                     /locus_tag="PKM34_02535"
     CDS             complement(6166..7638)
                     /locus_tag="PKM34_02535"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNQ82489.1"
                     /translation="MKKIIWIGLLLFFSFDSYSQLSLGLTNGNYSALGAGIINPSSMA
                     NTKLKAEIHVLSANGFVENNYLYFPSRTTTFLEIASGAYDYPLLPKPYGQGSRYVYSN
                     YEDKSSKNYFINTRVMGPSAMVTLNDHVFAIRTGVRAMSSTRRMPYDMANFCYYGMDF
                     KPQHRTLYFRDNYDMSAMAWWEVSLSYATVFNRGKSNLWSVGISVGPLFGYSGAYAKG
                     GDTKYLVYNGDILNVELLNAEFGYAIPVDDQTNEVDWADPLVRGTGWGMDIGVTWQYR
                     DKPYHRQISSYCYKKKFEEYQFKFGISLIDIGWINFYKEAEKHNFINVHNNWIRVNEL
                     DYNNIKDEIRTTSELFYNDSTASLTGNSFQIFLPASVGLQFDYHVKSAWYVNAVAVIP
                     AKYAEPMIERPMVIAVTPRYESRFIEVQLPLVLYDFKYPRFGLSLRIDGLTVGSDNLL
                     CFTSVKEFTGCDVYVSYRIMLRNDRKNPYTSKGACFNNWK"
     gene            complement(7750..>8331)
                     /locus_tag="PKM34_02540"
     CDS             complement(7750..>8331)
                     /locus_tag="PKM34_02540"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNQ82490.1"
                     /translation="YVTYDQNSQLHVYNDISKYWISIDTINYYNFNELTRTMSREFYG
                     NENTSFAGDKVRLLLPTALSIQGDYHIYRKWYGGLVWIQPVHLGKSYMRRPAQIALIP
                     RYESPKLELALPLSLYDYRMPRVGFSVRYYFLTIGTDDLLGLVGYKNFTGLDFYASIK
                     INFRKGFCGRFKRDLPCENDEYGIRSRGKNQRY"
BASE COUNT         2138 a         1938 c         1788 g         2467 t
ORIGIN      
        1 tctgttttgt gcctttccct gccaggtttc gttgtctgaa attggctggg actcaccata
       61 acctttgaat gttaatctat cggtgctgat tccatttccg gtgagataaa tatatacggc
      121 ttccgctctt ttccggctaa gttccaggtt gtattcatca gggccatcgc tatcggtatg
      181 acccccgatc tcgattctta tggaaggatt ttcattcaga aataaaagca gttttgtcag
      241 ttcggcagtc gattcggcag acaggatgta ctggtccttt tcaaagaata tatttctcag
      301 aatcattgta tttcccgggg caatcgggat taacccgatg tttttcacaa ccggattcac
      361 cgcatcatga ttttctttga tggggaagtg tgcggaataa aacaaataac cttcgcgcga
      421 cacattcagt gcataattcc tgttgcatgg aatgcacacc aggaattccc cgtttgcatc
      481 atcagattgt gcgataacga ccgtagtatc attttccgta tcgatcattt caaaatcagc
      541 ttttagcggc tgacgggtga tggaatcata gatcattccc ttaaaataag tgacagggac
      601 aggcctgact tcattattta aactgaaaga atatatatca tatccgccag atccttctaa
      661 agtgttgctg gagataaacc catacttacc actggcattc acgataatag caagttcatc
      721 tgcttcggta ttcagtggat atcccaggtt aacgggtttt gtccacgatt ggttgatctt
      781 tgatgacata aagaggtcag cacctcccat tcccggatgg cttttggaag agaagtacaa
      841 tgtctggttg tcaaaatgaa tgacaggaac catttccgcc tcgctgctat tgacaggtct
      901 gcctaaatta acaggcagtt gccaatgacc cagggagtcc ctttcggtaa cccagatatc
      961 ggaattcccc caccctcctg cccggttact ggcaaaatag atctttcttc catccggtga
     1021 tattgaaggc tgggcatccc aggattccga atttacattc ctgccgagat ttctggcgac
     1081 agaccatttc ccgttaattt tttcggagta gtacagatca caacttccat agctatcggc
     1141 tcggttgcag gctgtgaaca atatcatcct gccgtcgggc gacaggcaga atgatcctgc
     1201 atcattttct tttccaggtg gataggtaag caaagcggcc gaatcaccgg cattatttcc
     1261 gatttctgca atatataaat cctcagaata attccggcct tgaaccggtt ttggattctt
     1321 aattctcctt gtaaaaaaga taaactggcc atctgttgaa atggcattga tatattcatc
     1381 tcctgatgta tttattctta ttcccaggtt gacagggtta aatgcaacag gtgcagccct
     1441 taaggcggac ctggttatcg cctgcccgag tctttcctgt gcagcctgtg tcggtttgct
     1501 gtcgatcccg gaaattttta agatggtatc gtaatatact ttggattcct catattcttt
     1561 tagtgaaaat agtgtatttg ccaaaacata acacaatgat tcccagcgct caggattccg
     1621 ttccagcgcc ttcttataat agattactgc ctgtcccggt tcggattgtt ccatcaggac
     1681 ttctgccatc attacgtacg gttcggcatt tgttgaatca tacctgaagg ctgcgttcaa
     1741 ttcagctttg catttatccc attcctgccg ggaataatat tgccaggctt tttcgaccgc
     1801 tttttccgat ttttgtttat taatctcctg cgaatggctg caaacgggaa acaggaccag
     1861 gaataagagc aacagactca accactgccg gaaggacttt tttaccttta cgggatattc
     1921 atgaacttgt gtgattgcag ggatatcttc cattgcggat gggtttttat ataattaacg
     1981 atggaaggca gtatctgatt atacctgctc cattccggct gcaaatacag gtggcatttt
     2041 ccggttattt tttccgcatt cagttcagcc caacggaaat cgctctcctg gtgtatgatg
     2101 actttaagtt cggcagcttc cctgaaatat tcaggcagtg gaagggaacc tttttttggt
     2161 gaaaggcata accaatccca cgatcccgat agcggcctga cccctgctgt ttcaaggaat
     2221 gtttcaattc cattttcttt tagcttttta caaagatagt caaggttgta aagtacgggt
     2281 tctccgcccg tcaccaccac agattttgcc ggttgctgca gtacccggta taccatctca
     2341 tcggtatctg tcaaaggatg cagacgggcg tcccaggatt cttttacatc acaccaccag
     2401 caacccatat cgcatccccc gaggcggata aaataggctg ctttacccgt attgaatcct
     2461 tcaccctgga tggaataaaa atcttccatc agcgggagca gtttcccttg ctcaagtaaa
     2521 tttctcagat ccggcgaaag tggcgtattc attatagatc agccgtatat ctcttttttc
     2581 gcggctttta ccgtattcat cagcaacgag actatagtca tcaggccaac gccacccggc
     2641 accggagtga tcatagaagc aattttcgaa acttcttcaa agtttacgtc ccctgtgagc
     2701 cggtatcctg attgggttgt atctgaaggg atccggtgca taccgacatc aatgaccacg
     2761 gctcctttct tgaccatttc tcctgtgaca aagccgggct gcccgatggc tgcaatgaga
     2821 atatccgctt ctttagccag tttaccgaga tcttttgtct tgctgtggca aagagtgacc
     2881 gtacaatttc cgggataatt tttccgtgac atcaggatag agacaggtgt accaacaata
     2941 ttgctcctgc ccagcacgac acaatgtttt ccttccgttt cgacgcggta atgttcaagt
     3001 aattttatta taccataagg tgtagccggc agatacgaag gcaaaccctg taccattcta
     3061 ccgatattga ccggatgaaa accatccaca tcttttttcg gatcgattgc ctggacaatt
     3121 ttttccggat tgatatgccc ctggagcggt aattgcacga tcaggccatg aacctccgga
     3181 tcattattga taaaatcgat catggccagc agttcatttt cgctaatacc ggccgattgc
     3241 ctgtaaacag aagaaatgat acctgcttca tggcaggctt tttccttgtt cctgacatag
     3301 gtctggcttg ccgggtcttc accgacgaga atagcggcca ggtggggatc tgtacccgag
     3361 tctatcaggc tggccacttc aattttcaat tcttctatta tttgctgtga taccagcttg
     3421 ccatccaata atttcatttt atatcatttt atcctgaaag aaaatcatgc aaataccggt
     3481 catttatttc cggccctgag acttcatgtt ttgcatggcg cgcatcatat tcttcccttt
     3541 tccggccgac atcattttca tcattttact ggtttcatag aattgcttga tcagcctgtt
     3601 aacttcctgg atatcattgc cgctgccaat tgcgatccgt tttctcctgg acccgttcag
     3661 caacttaggg ttttctcttt catcgggtgt catggaataa atgatcgctt cgattccttt
     3721 gaaggcattg tcatcaatat cgacatcacg aagtgctttt cccatgcccg ggatcatact
     3781 catgaggtct ttcacattac ccattttctt gatctgtttg atctgggtca tgaagtcatt
     3841 gaagttgaac tcatcatggg caattttttt ctgaagtttt ctcgcttctt cttcgtcgaa
     3901 ctgttcctgg gcgcgttcaa caagcgaaac gatatcgccc attcccagga ttctgtccgc
     3961 cattcgggaa ggataaaaga cgtcgatggc gtctatcttt tccccgattc caacgaattt
     4021 gataggtttc gttacgacag cgcggatagt cagggcagcc ccaccccggg tatccccatc
     4081 gagcttggtc agcacaacac catcgaaatc aagccgttca ttaaaggctt tggccgtatt
     4141 aacggcatcc tgcccggtca tggcatccac tacaaacaag gtttcgttcg gctgaacccc
     4201 atttttaatg gctgcgatct ctttcatcat ttcttcatcg atggctaacc gtcctgccgt
     4261 atcgatgata accacgttgt agtttttatc tttagcatat tgaactgcgc ggcgggcaat
     4321 ctgaaccgga tcccggttat cgggttctga atagacttcg acccctattt gttctccgag
     4381 cacttttatc tgttcgatgg cagcaggccg gtaaatatcg caggctgcca tcatgacctg
     4441 cctgcctttt ttcgatttga gataatgagc cagttttgct gagaaggtag ttttcccgga
     4501 accctgaaga cctgacatca ggatgatcgc gggctttccc tgggtattca gctcggcctg
     4561 gctgcttccc atcagttccg ccagtttgtc gtggactatt ttgaccatca gctggctggg
     4621 tgaaacggat gtcaggattt tctgacccat ggcctcctgt tttacctggt cagtaaaatc
     4681 ctttgcgatt ttatagctga catcggcatc cagcagggcc ttgcgaacct ctttaagggt
     4741 ttcggccaca ttgatttcgg tgatgtgtcc ctgccctttc agtatccgga aagatctttc
     4801 cagtttatct gataatcctt cgaacataac agaattaaat tttgtgcaaa agtattaaat
     4861 aaaaacctca caaaccagcc ggttcgtgag gtcaatgggt gcgtgaaggg aatcgaaccc
     4921 tcagccctcg gaaccacaat ccgatgctct aaccgttgag ctacacacac cgtttaaaag
     4981 cggcagcaaa gatatgaaaa aaaataaaaa attctgcaga ctccattata tttgttgata
     5041 gaaacctgaa acaaaccagg gtataaaagg taaagatggt ttttgagaaa ctcaaaaata
     5101 acaagcccgc cattgcctcc gtctcgttct tgatcatggt ctttgtcgtt gctatggcag
     5161 gatatcttat cacacccgat ccgacgccgt tggccaaccg tcagttcctg gaattgggcg
     5221 cagccaggcc gggtttttcc gtgactatgc taaaactttc agatacggga tttcatgaaa
     5281 aatcaggatt aatcagaaag tggatatacg ggcaacgcac tccctatcga tatatcccgg
     5341 taaaaaactt cttccgggaa ggcggtaata ttatcgcaga ggcctatacc ggagatgaat
     5401 cgcaggggca agaattgagg attgatacaa aaggcagcag ttttgacaag tttatcattg
     5461 ttaaacggtt tcccttgggg acggatcaat tcggacggga tttgttaagc cggttgatca
     5521 tcggggcgag aatcagcttt gcggtagggt tagtatccgt tgtaatttcg cttttcctgg
     5581 gcgttactat cggggctgcc ggcggatatt tcggtggccg ggttgataaa tttgtggtat
     5641 ggctgatcaa tgtcttatgg tctgtcccga ccctgctgct ggtaattgcc attactttcg
     5701 cgctgggaaa gggattctgg caaatttttg ttgctgttgg atttaccatg tgggtggagg
     5761 tagccagaat tacccgtgga cagatccttt cggtaaggga aaaggaatac atcgaagcag
     5821 cagaagcgct gggattctcc agattccgca tcattatcag gcatatcctg cccaacattg
     5881 ccggccccat tattgtggtt gcagctgcta attttgccac tgccatcctg gtggaagccg
     5941 gcttaagttt cctggggatc ggtgtccaac ctcccatgcc ttcctgggga aatatgatca
     6001 gggagaatta tcatttttta ttgctgggag aatatcattt agctcttttt ccgggattag
     6061 tactgatgct gaccgtatta tcatgcatga tactgggcaa tggaattcgc gacgcaatgg
     6121 atgtccggtc atccggcact atggtcagtt agaaaattga tcttactact tccagttatt
     6181 gaaacaggct ccttttgaag tatatggatt cttcctgtcg ttcctcaaca taatccggta
     6241 tgacacatat acatcacagc ctgtgaattc tttgacagag gtaaagcaca gcaagttatc
     6301 ggaaccgact gtaagaccgt caatccgcaa tgataagcca aaccggggat atttaaaatc
     6361 atagaggacc aaaggcagtt gaacctcaat aaagcggctt tcataccttg gcgtaacggc
     6421 tattaccata ggcctttcta tcatcggctc tgcgtatttc gcaggtatta cagcaacagc
     6481 attaacatac catgctgatt ttacgtgata atcaaactgc agtccgaccg atgccggaag
     6541 aaagatctgg aacgaattac ccgttagaga agctgtggaa tcattgtaga ataattcgct
     6601 ggttgttctg atctcatctt ttatattatt gtaatccagc tcattaactc gtatccagtt
     6661 attgtgaaca ttaataaaat tatgtttttc cgcttcctta taaaaattta tccaaccgat
     6721 atcgatcaaa gaaattccaa acttaaactg gtactcttca aactttttct tgtagcaata
     6781 gcttgatatc tgtctgtggt agggtttatc acgatattgc caggtaacgc cgatatccat
     6841 gccccagcct gtacccctga cgagcggatc ggcccaatca acttcattcg tttgatcatc
     6901 taccggaatt gcatatccaa attctgcgtt taaaagttcg acattaagga tatcaccgtt
     6961 gtataccaga tattttgtgt cacctccttt ggcatatgcc ccgctgtatc cgaacaatgg
     7021 tccgacagaa atgccaacag accacaagtt acttttgccc cggttaaaaa cggttgcata
     7081 gctaaggctc acctcccacc aggccatggc gctcatatca taattgtccc tgaagtataa
     7141 agtgcggtgc tgcggtttga aatccatccc ataatagcaa aagtttgcca tatcataggg
     7201 cattcttcgg gttgacgaca tagcccttac cccggtcctt atggcaaaaa catgatcatt
     7261 cagagtgacc atggctgaag gtcccattac ccgggtattg ataaaataat tcttggagga
     7321 tttatcttca taattagaat aaacatacct gctgccctgt ccataaggtt ttggcaacaa
     7381 cggataatcg taagcgccac tggcaatttc gaggaatgtt gtcgtccttg aaggaaagta
     7441 caaataattg ttctctacaa atccattggc tgataagaca tggatctctg ctttaagttt
     7501 tgtattggcc attgaagatg gatttattat tccggcgcct aaagccgaat aatttccgtt
     7561 tgtcaatccc aacgacaatt gagaatatga atcaaaggag aaaaacagca gcagtcctat
     7621 ccaaatgatc tttttcatcg caaaataaaa tgcgttatca ttttgatatt catccggcaa
     7681 cagatgaatg gtaatacata ttttacttga aaactggagg aatgttgcgt aatttggaga
     7741 gattataact taatagcgct gattcttacc ccggctgcgg atgccatact catcattttc
     7801 acagggtaaa tcgcgtttga atctgccgca aaatccctta cggaaattaa tcttaatcga
     7861 agcgtagaaa tcaagaccgg taaagttttt atatcccaca agccccagta aatcatcggt
     7921 accaatagta aggaaataat agcgaaccga aaaaccgacc cgcggcatgc ggtagtcgta
     7981 cagggatagc ggtaaagcca gttcaagttt tggtgactca tacctcggaa tcagggctat
     8041 ctgggcaggc cgcctcatat aggatttacc caggtgcaca ggttgaatcc agaccaatcc
     8101 tccataccat tttcggtata tatggtaatc cccctggatg ctcaaagcag ttggtaacag
     8161 cagtcttacc ttgtctccgg cgaaggaggt attttcattg ccatagaatt ccctgctcat
     8221 tgtccgggtc agctcgttga aattataata gttgatcgtg tcaatactga tccagtattt
     8281 actgatgtca ttataaacat gcaactgcga attctggtca taggttacat a
//
LOCUS       DAOQXS010000050         8331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9034_length_8331_cov_3.120469, whole genome shotgun sequence.
ACCESSION   DAOQXS010000050 DAOQXS010000000
VERSION     DAOQXS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9034_length_8331_cov_3.120469"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1651
                     /locus_tag="PKM34_02545"
     CDS             329..1651
                     /locus_tag="PKM34_02545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNQ82491.1"
                     /translation="MKVTIVGTGYVGLVTGACFAEVGIDVTCVDIDKTKIDNLVKGII
                     PIYEPGLEEIVQRNVTKGRLHFTTNLAETLNGCEVIFSAVGTPPDEDGSADLSYVINV
                     AREVGRNMSDYLLIVTKSTVPVGTAAKVRQAIQAELDSRGVDIPFDVASNPEFLKEGA
                     AVDDFLKPDRIVVGIDSQRAEDLMKKLYKPFTLNGHPVIFMDIPSAEMTKYAANAMLA
                     TRISFMNDIANLCEIMGADVTMVRKGIGSDPRIGPKFLYPGVGYGGSCFPKDVKALIK
                     TAKDNNYDLRVLQAVEQVNDDQKSVLFDKVKLYFNGQLQNKTIAIWGLSFKPETDDMR
                     EAPALVLIKKFLAAGCKMKVYDPIAMNETKRKIGDIVYYATDEYDALQAADCLLLVTE
                     WKEFRVPDKELMKQKMKNYAIFDGRNIYDGHEMIAAGFDYFSIGKNWK"
     gene            1654..2589
                     /locus_tag="PKM34_02550"
     CDS             1654..2589
                     /locus_tag="PKM34_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ82492.1"
                     /translation="MRKRILVTGGAGFLGSHLVEKLLNEGNDVICVDNYFTGQKRNIA
                     HLIGNPYFEVIRHDITMPFFIEVDEIYNLACPASPIHYQFNPIKTIKTSVMGSINMLG
                     LAKRIRAKILQASTSEVYGDPEIHPQHESYWGHVNPIGSRACYDEGKRCAEALFMNYH
                     HQNNVRIKIMRIFNTYGPRMHPNDGRVVSNFIVQALNNEDITIYGDGMQTRSFCYVDD
                     LLDGMTRLMATGDDFIGPVNVGNPGEFKILELAEKVISLTKTRSKIIFKALPEDDPMQ
                     RQPDITLARKVLGWEPQIPLEEGLIRTIDYFKSIL"
     gene            2590..3459
                     /gene="rfbD"
                     /locus_tag="PKM34_02555"
     CDS             2590..3459
                     /gene="rfbD"
                     /locus_tag="PKM34_02555"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNQ82493.1"
                     /translation="MVKILITGSNGQLGSEFRAIAREHPHFRFIFTDVGELDITNSNR
                     LDEFFRSEAPDVVINCAAYTAVDKAEDEPEKAIWINRDAVANLAGACSLYDTFMVHIS
                     TDYVFDGNSSRPYTENDIPSPTSVYGLSKLDGEESMQACLQKGLIIRTSWLYSSFGNN
                     FVKTILRRCNQPSSLGIVTDQTGSPTYGRDLARAILNILPDAMASNELEILHYSNEGS
                     CTWYELAQEIVKLSATGCRIHPILTKDFKSRAVRPVYSVLDNTKARKKYGLTIPGWKV
                     SLKECITLLKESE"
     gene            3503..5233
                     /locus_tag="PKM34_02560"
     CDS             3503..5233
                     /locus_tag="PKM34_02560"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNQ82494.1"
                     /translation="MEQLILEKAQTWLGEGFDDETRKAVKDMIDNNPQELVESFYQDL
                     EFGTGGLRGIMGVGTNRMNKYTVGMATQGLANYLKKIFPDKNPVRVAIAYDSRNNSPY
                     FAKITAEVFSANNIRVYLFHSLRPTPELSFAIRHLNCQSGIVITASHNPKEYNGYKAY
                     WEDGSQLIPPHDKNVIAEVQKITSVSDVRFEGIPENIEMIGEEIDKVYLEKVKSLSLS
                     PAAIQEHKDLKIVYTPLHGTGGEMVPAALKKFGFENISVVAAQAITDGNFPTVKSPNP
                     EEPAALEMAIAQAEKIGADIVMATDPDADRVGIAVRDLQGRFTLLNGNQTAALLTYYL
                     LLKWKENKKLKGNEFIVKTIVTSELIKEIADAFGVECFDVLTGFKWIAEIIRLNEGNK
                     TFIGGGEESYGYMTGDFVRDKDAVSSCALVAETAAWAATRGKSLYELLIDIYLEFGLF
                     RESLISVTKKGKSGSEEIRKMMDIFRNTPPQIINGSKVILIKDYKSSEAHDLLTGEKS
                     KINLPASNVLQFFMEDGSKISVRPSGTEPKIKFYFGVMNELNSRSDFEKINDILDQRI
                     RNIINDLKLV"
     gene            complement(5244..5942)
                     /locus_tag="PKM34_02565"
     CDS             complement(5244..5942)
                     /locus_tag="PKM34_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ82495.1"
                     /translation="MSTSLSTISSCTINPHQCRCFDMLSEDERILLESNSVRVRYRKK
                     EIICKQGSFVSHVVHVEKGLAKVYLDDGDNTLILKIIPQGNLLGLTSLSEDYNTYQYS
                     AMAYVDTEVRQIDINIFRQLIESNARFAREVIDILSSNSVQIYSRFFCLTHKQAFGRL
                     ADILLCLANRIFKSQEFELPLSRKDLAELSGMSQETVVRILKNFDDEGLIRSEGKSIH
                     IVEYEKLEQISRKG"
     gene            6130..7470
                     /locus_tag="PKM34_02570"
     CDS             6130..7470
                     /locus_tag="PKM34_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82496.1"
                     /translation="MKYIMMITMVILSALIIGCSKDNNNDDKAVASCEGCHTNYAHLQ
                     EVYSPDTAAPVGGCGGDAPHYEPYDRVYMGGEGFEAYKTSGHYELGCIACHNGVDNTA
                     DKAVAHSGDFIRHPSAYAEEKCGSCHKQIVENFRTSIHNGTGQKRKVAIRSGLNGPDD
                     FDQLPAHQIEGYTNNCATCHGTCGNCHIVRPPIGGGGLASGHSFKKTPDMVNVCVACH
                     VSRGGHAYLGIASGTVPDVHLTKMQFTCMDCHSGDELHGDGVKVEQRYAYSKLPECEE
                     CHAGLAGENMYHDVHYDDFNCQVCHSQDYNNCGSCHIHGDGARVPSYMSFKIASNPLP
                     DIKTGFDFALVRRTLAAPDNWEKYGVSEYADFDALPTYNYTSPHNILRWTKRTSNEAG
                     KACYTKCHIRNEGGTLVNKELYLFRSDLLEWEMNATTGITVDGKLPAGWFEEKN"
     gene            7515..>8331
                     /locus_tag="PKM34_02575"
     CDS             7515..>8331
                     /locus_tag="PKM34_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNQ82497.1"
                     /translation="MTKFKFLLLSVLFAALFFTACEKESTVNEAQVLAEYLESTDSPA
                     GKDFVNTDMPTIISATDVKTLNETNKVYIIDIRAAEDFNNGHIKNAHNVPLKDIVTHI
                     ESVDLAGYDKVAVVCYTGQTAGFATSLLRLLGYEKAFSMKWGMCSWNEAFAGKWNTAI
                     ANGNAYASQFTATATDKGPAGELPELSTGKETGQEILEARVNALLTEGFTPATVTNQT
                     VFGALSSYYIVNYWPADQYAAGRGQPSFDKQFVRDYLDTLDWDKTPPAPALPPE"
BASE COUNT         2410 a         1885 c         1949 g         2087 t
ORIGIN      
        1 cggatgcttt gccggcgaat aaaccattcg ctggtgctgc gcagacattg cgatcgaaat
       61 aagcaatgtt gccaagagta aagttgattt cttcatagtt ataatagttt ggcgttaagt
      121 aattatcaaa acaaagaacg tgctagaaac agctgtttta tcatgccgat tattgaattc
      181 tcgaaattat taaaattaca atgtcgtgta ccttaaaaaa tattaaaaaa ttccaaccaa
      241 ttcataaatg ccaaaagccg gacttgatac cgcattttcc ttgacgatta tttttacctt
      301 tgccgcttca ttaaatagat tcgtaaatat gaaggtaact attgtcggaa ccggatatgt
      361 tggtctcgta accggggcgt gttttgcaga agtaggtatc gatgtaacat gtgttgacat
      421 cgataaaaca aagattgata acctggtaaa gggaatcatc ccaatctatg aacccggact
      481 ggaagaaatc gttcagcgga atgtaaccaa aggccgcctg cacttcacca cgaatttagc
      541 ggaaacgctc aacggatgtg aggtgatctt cagcgctgtc ggcacacctc ctgatgaaga
      601 cggcagtgca gacctttcct atgtaataaa tgtggccagg gaagtaggac ggaatatgag
      661 cgattacctt ttgatcgtca ccaaaagcac ggtaccggta ggaactgcgg caaaagtcag
      721 acaggctatc caggcagaac ttgatagccg aggcgttgat attcctttcg acgttgcatc
      781 aaaccccgaa ttcctgaaag agggtgcggc tgttgatgat ttcctgaaac ctgacaggat
      841 cgtcgtgggg atcgattcgc aaagggcgga agacctgatg aaaaagcttt ataaaccatt
      901 taccctgaac ggacatcccg ttattttcat ggacatccct tcggcggaaa tgacaaagta
      961 cgcggctaat gcgatgctgg caacgcgtat cagctttatg aatgatattg ccaacctctg
     1021 cgagatcatg ggggccgatg taaccatggt gcgaaaaggc atcggaagcg atccccgtat
     1081 cgggccgaaa tttctatatc ccggagttgg ctacggggga tcctgctttc caaaagatgt
     1141 gaaagcactg attaaaacag ctaaggataa caactatgat ctcagggttt tacaagctgt
     1201 tgaacaggtg aatgatgatc agaaatccgt tctctttgat aaagtcaaac tctatttcaa
     1261 tggccagttg cagaataaaa ccatcgccat ctggggcctg tcgtttaaac ccgaaacgga
     1321 tgatatgcgt gaggcgcctg ccctggtcct gattaaaaaa ttcctggccg ccggatgcaa
     1381 gatgaaggtc tacgatccaa ttgctatgaa tgaaacaaaa agaaaaatcg gggatatcgt
     1441 ctattacgcc accgacgaat acgacgcgct tcaggctgcc gattgccttc tgctggttac
     1501 ggaatggaag gaattccgtg tcccggataa agaattgatg aagcagaaaa tgaaaaatta
     1561 tgctatcttc gacggtagaa atatctacga cggccatgag atgattgctg caggatttga
     1621 ttatttctcc atcgggaaaa actggaaata aaaatgcgaa aaaggatcct ggttaccggc
     1681 ggcgccgggt ttttaggttc acaccttgtc gaaaagcttt tgaatgaagg caatgacgtg
     1741 atttgtgtgg acaattattt taccggccag aaacgcaata ttgcccattt aataggaaac
     1801 ccgtatttcg aggtgatccg gcatgatata accatgcctt tttttattga ggtagatgaa
     1861 atttataatc tggcctgtcc tgcctcgcct atccactatc agttcaaccc cattaaaacg
     1921 atcaaaacat cggtcatggg ttcgataaat atgctgggac tggccaaaag gatcagggcg
     1981 aaaatcctgc aggcctccac atcagaagtc tatggtgacc ccgagatcca ccctcagcac
     2041 gaatcctact ggggccacgt caatcccatc ggctccaggg cttgttatga tgaaggcaaa
     2101 cgttgcgccg aggcgctgtt tatgaactat caccaccaga ataacgtccg gatcaagata
     2161 atgcgaatct ttaacactta cggtccgaga atgcacccga acgatgggcg ggttgtttca
     2221 aattttatcg tacaggcgct taataatgaa gatattacaa tctatggaga tgggatgcaa
     2281 acccggagtt tttgttatgt cgacgatctt ttggatggaa tgaccaggct tatggctacc
     2341 ggcgatgatt tcatcggccc tgtcaatgtc ggtaaccccg gggaatttaa aatactggaa
     2401 ctggctgaaa aagtaatatc cctgacaaaa acacgttcta aaattatctt caaggctttg
     2461 cccgaagatg acccgatgca gcgccagccc gatattaccc tggccaggaa agttttgggg
     2521 tgggaacctc aaataccgct tgaagaaggg cttatcagaa ccatcgacta tttcaaatcc
     2581 atcttgtaat tggttaaaat cctcattacc gggagcaacg gccagcttgg cagtgaattc
     2641 agggcgattg cccgtgaaca tccgcatttc cggtttatct ttactgatgt aggtgagtta
     2701 gacattacta acagcaaccg tctggacgaa ttcttccggt cggaagcacc ggatgttgta
     2761 atcaactgtg ccgcttatac tgctgttgat aaagcagaag atgaacctga aaaggccatc
     2821 tggatcaaca gggatgcagt ggccaacctc gctggtgcct gtagcctcta tgacacgttc
     2881 atggttcata tatctacgga ttacgttttt gatggtaatt ccagccggcc atataccgaa
     2941 aacgatattc cctctcctac ctcagtatac ggattatcaa aacttgacgg cgaagaatca
     3001 atgcaggcct gcctgcaaaa agggctgatt atcagaactt cctggcttta ttcgtcattt
     3061 ggtaataatt ttgttaaaac gatcctgaga agatgcaatc aaccatccag cctgggcatc
     3121 gttaccgatc agaccggttc tccaacgtac ggccgcgatc tggccagggc aatccttaac
     3181 attttaccgg atgcgatggc ctcaaatgaa ttagagattc tgcactattc aaatgagggt
     3241 tcctgcacct ggtatgaatt ggcccaggaa attgtaaaat tatcggcaac cgggtgccgg
     3301 atccatccta tcttaaccaa agatttcaaa tcgagggctg tccgtcccgt ctacagcgta
     3361 ctggataata cgaaagcaag gaaaaaatat gggttaacca tccccggttg gaaagtcagc
     3421 ctgaaagaat gcataacatt acttaaagaa tctgaataaa ccaatttaaa ctgaaagtaa
     3481 tactgaagga aataaagcat ttatggaaca attaattctt gaaaaagcac aaacgtggtt
     3541 aggcgaaggt ttcgatgatg aaacccgtaa agccgtgaaa gacatgatcg ataataatcc
     3601 ccaggaatta gtggagtcgt tttaccagga tctggagttc ggaacaggtg gcctcagggg
     3661 aattatgggt gtaggaacca acaggatgaa caaatacacc gtcggaatgg ctactcaagg
     3721 gctggcaaac tatctgaaga agatttttcc ggataagaat cctgtcaggg tggccatcgc
     3781 ctacgacagc cggaataata gtccgtattt tgccaaaatt actgccgaag ttttctcggc
     3841 caataatatc cgggtatatc ttttccattc tctccggcca actcccgagc tatcgttcgc
     3901 cataaggcat ctgaattgcc agagcgggat cgttattacc gcttcacaca atcccaaaga
     3961 gtataatgga tataaagctt actgggaaga tggaagccaa ttgattccac ctcacgataa
     4021 gaatgtgatc gcagaggtgc aaaaaataac atccgtttct gatgtgagat tcgaaggaat
     4081 tcccgaaaat attgagatga tcggggaaga aatcgacaag gtttacctgg agaaagtcaa
     4141 atcgctttcc ctctcccctg ccgccattca ggaacataaa gacctgaaaa tcgtctacac
     4201 gcctttgcat ggaaccggcg gggagatggt cccggctgcc ctcaaaaagt tcggatttga
     4261 aaacatttca gtcgttgcag cccaggcaat aaccgacgga aatttcccga cggtcaagtc
     4321 gcccaacccg gaagagccgg cagcccttga gatggctatt gcacaagcgg agaagatcgg
     4381 cgccgatatc gttatggcca cagatccgga tgccgatcgc gtagggatcg ccgtccgcga
     4441 cctccagggc cggttcaccc tgcttaatgg caaccagacc gcggccctgc ttacttatta
     4501 ccttcttttg aaatggaaag aaaataaaaa actgaaagga aatgaattta tcgtcaaaac
     4561 cattgtcacc tcggaattga tcaaagaaat tgccgacgct ttcggtgtag agtgttttga
     4621 tgtgctgacc ggttttaaat ggatcgccga aatcatccgt ctcaacgaag gaaataaaac
     4681 ttttattggc ggaggagaag agagttacgg ttatatgacg ggcgattttg tcagggataa
     4741 agatgctgtc agttcatgcg cactggtggc cgaaacagcc gcatgggctg caacaagggg
     4801 taaatcgcta tacgagctct taattgatat ttatcttgag ttcggcctgt tcagggaatc
     4861 tttgatctcg gtgacaaaaa agggcaagtc tggcagcgaa gagatcagaa aaatgatgga
     4921 catcttccgg aatacacccc cgcaaataat caacggatct aaagtgatcc tgatcaaaga
     4981 ttataaatct tccgaagccc atgatctgct gaccggtgag aaatcaaaga tcaatttgcc
     5041 ggcctcaaat gtattgcagt ttttcatgga agatggcagt aaaataagcg ttaggcccag
     5101 cggaacggag cctaagatca agttttactt tggcgtcatg aacgaactta attcgagatc
     5161 ggattttgaa aaaatcaatg atatcctcga tcagcggatc agaaatatca ttaatgattt
     5221 gaagctggtt taagagggaa tgatcagcct ttccggctta tttgctccag cttttcgtac
     5281 tcaacaatat ggatggattt tccctctgac cggatcaatc cttcatcatc aaaattcttt
     5341 aggatcctca cgaccgtctc ctggctcatt cccgatagtt cggcgagatc cttcctggat
     5401 agtggaagtt caaattcctg gcttttaaag atcctgttag ccaggcataa caagatatcg
     5461 gccagcctgc cgaaagcctg cttgtgcgta aggcagaaaa aacggctgta aatctgaaca
     5521 ctgttggaac tgagaatatc gatcacttcc cgggcaaacc gggcattgct ttcaatcaat
     5581 tgcctgaaaa tattaatgtc gatctgccgt acttctgtat ccacataagc catggctgaa
     5641 tactggtaag tattataatc ttcggaaagg gatgtcagcc ccagtaaatt tccctggggt
     5701 ataatcttca gaataagcgt attatcgcca tcatccaaat aaacttttgc caatcctttt
     5761 tctacgtgca cgacatgtga cacaaaactt ccctgcttgc aaatgatctc cttcttccgg
     5821 tacctgaccc tcaccgaatt ggattccagc aaaatccgct catcttcaga aagcatatca
     5881 aaacaccggc actgatgcgg attaatcgtg caactggaga tcgttgataa tgaagtactc
     5941 atttggaatt attattgggc aaatatataa aattaaacat gtattttttt gatccggatc
     6001 aatgattctc ctgtcctgaa acaatcgaaa ggccatttca tctctattga taaataaata
     6061 acaacagatc aaaataaaca acctttgtaa tatgtaacta tattaaggtt tatgaaaatc
     6121 tgctatgata tgaagtatat tatgatgatc acaatggtta tcctgtccgc attaatcatc
     6181 gggtgttcaa aggataataa caatgatgac aaagcggtag cctcttgtga aggctgtcat
     6241 accaattatg cccacctgca ggaagtttat tcgcccgata cggcggcgcc agttggcggc
     6301 tgcggtggtg acgcccccca ttatgagcca tacgaccggg tgtatatggg tggcgaagga
     6361 ttcgaagctt ataaaacatc cggacattat gagctgggct gcatcgcttg ccacaatggc
     6421 gtggataaca ctgcggataa agcggttgcc cattcggggg atttcatccg gcatccttcg
     6481 gcatatgcag aagagaaatg cggatcatgc cataagcaga tcgtggagaa tttcaggacc
     6541 agtattcaca acggcaccgg ccagaaaaga aaagtggcta tcagaagcgg actaaacggc
     6601 cctgatgact ttgaccagct gcccgcccac cagattgaag gatacacgaa taattgcgcc
     6661 acctgccacg gcacctgtgg taactgccat atcgttcgcc cgccgattgg cggtggcgga
     6721 ctggcttccg ggcattcttt caagaaaacc ccggatatgg tcaacgtgtg tgtagcctgc
     6781 catgtttcac gcggcggcca tgcctacctg ggtatcgctt ccggaacggt accggatgtt
     6841 cacctgacaa agatgcaatt tacctgcatg gattgccaca gcggcgatga acttcacggt
     6901 gatggcgtga aagtagaaca gcgctatgct tattcaaaac ttccggaatg cgaagagtgt
     6961 catgccggcc ttgccggtga gaacatgtac catgatgttc attacgatga tttcaattgc
     7021 caggtttgcc attcacagga ttataacaat tgcggaagtt gccatattca cggcgacgga
     7081 gcccgggtac cttcttatat gagcttcaaa attgcctcca atccgctgcc ggatatcaag
     7141 accggtttcg acttcgccct ggtacgccgg acgctggccg ccccggataa ctgggaaaaa
     7201 tacggggttt cggaatatgc tgatttcgat gccttgccga cctataacta tacctcccct
     7261 cataatatct taagatggac caagaggacg tcgaacgagg cgggaaaagc ctgttataca
     7321 aaatgccata tccgcaacga aggcgggaca ctggtcaaca aggaactcta cctgttccgg
     7381 agcgacctgc tggaatggga aatgaatgca actaccggta ttacggtcga cggaaaactt
     7441 ccggccggct ggtttgaaga aaagaattga atttcaataa attaattaat gactaactaa
     7501 caaaaattaa acctatgaca aaatttaaat ttctgctttt aagcgttttg tttgcagctt
     7561 tgttcttcac agcatgcgaa aaagaatcaa ctgtcaatga agcgcaggtt ctggctgaat
     7621 accttgagtc gaccgattca ccggccggaa aagatttcgt aaacaccgac atgccgacta
     7681 ttatttcggc gaccgatgtt aaaaccctga atgaaaccaa taaggtttat attatcgata
     7741 tccgtgcagc cgaagatttt aacaatggcc atatcaagaa cgcccacaat gtacctctca
     7801 aagatattgt cacccacatt gaatcagtag accttgcggg atacgataaa gtagcggttg
     7861 tttgttatac agggcaaacc gcaggctttg cgacatcgtt gctcaggttg ttgggctacg
     7921 aaaaagcttt ttcgatgaaa tggggtatgt gttcctggaa tgaagctttt gccggtaaat
     7981 ggaatactgc tattgccaat ggaaatgcct atgccagcca gttcacggct acggccaccg
     8041 acaaaggccc ggccggcgaa ctccccgaac tgtcgaccgg taaagaaacc ggccaggaga
     8101 tcctggaagc ccgcgtcaat gcgctgctga ccgaaggatt taccccggca acggtaacca
     8161 accagacggt tttcggagct ctttcgagct attatatcgt caattactgg ccggccgacc
     8221 agtatgccgc gggccgcggc cagccgagtt tcgacaagca gtttgtgcgc gattatctcg
     8281 atacgctcga ttgggacaag actccgcccg cgccggccct tccgcccgag g
//
LOCUS       DAOQXS010000051         8243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9155_length_8243_cov_3.864191, whole genome shotgun sequence.
ACCESSION   DAOQXS010000051 DAOQXS010000000
VERSION     DAOQXS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9155_length_8243_cov_3.864191"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKM34_02580"
     CDS             <1..245
                     /locus_tag="PKM34_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82498.1"
                     /translation="KGIGISPENQRHIFRNLYRVPTGNLHKVKGFGIGLFYVKTIVEA
                     HGGHINLTSELNKGSRFDLFLPFVYNLNKEDEYTQT"
     gene            226..933
                     /locus_tag="PKM34_02585"
     CDS             226..933
                     /locus_tag="PKM34_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ82499.1"
                     /translation="MNTPKPSILLVEDDPNLSLVLQDYLEMMNFDIHLCRNGKEGLDA
                     FRHRKFNICILDIMMPVMDGFTLAEEIRKTDDTVPIVFLTAKSLKEDRIKGFRMGCDD
                     YITKPFSTEELSLRIQAILKRCMNQPARAEKEEPPVYNLGNYIFDHKNLILSINGEQK
                     VLTRKENALLKLLFEHQNQLLPREVALKSVWGNDDYFIGRSMDVFITKLRKYLKDDPS
                     IFITNVHGTGFKLEVKK"
     gene            complement(934..2970)
                     /locus_tag="PKM34_02590"
     CDS             complement(934..2970)
                     /locus_tag="PKM34_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNQ82500.1"
                     /translation="MDAPIAKKEIKELTIHGQTRIDNYYWLNQRENPDVVAYLEAENK
                     YTKAMMAHTDGLREKLFTEITGRIKQTDMSVPYVENGYYYYTRYEEGKEYPLYCRKKG
                     SLDSVEEIMLNVNEMAAGYAYYDVGGLEVSDDNRLLAFSVDTVSRRIYTLYFKNLGTG
                     EIYPDRIGQTSGNLAWAADNKTLFYSTRDESLRPDKIWKYRLGQSEKESELVYFEEDV
                     TYNTFVYRSKSRKYLMIGSGSTMSDEYRYMDAGDPNGRFTVFQPRIRGLEYSVAHYED
                     RFYIRTNDNAVNFRLMETPEGATGRENWKEVIGHREDVLLEDFDVFKGFLAIQERKEG
                     LKTIRIFNHAGLDYYLDFTEQAYTVTLGSNEEMETEVVRYNYTSLTTPMTVFDYNTRT
                     RESEMKKRQEVVGGYEPGDYFTERVWVTASDGKQIPLSMVYRKDLKRDGHVPVLLYGY
                     GSYGYSTDPYFNSVRLSLLDRGFIYAIAHIRGGEEMGRMWYEDGKLMNKKNTFTDFIA
                     CGEYLVNRGYTNPGQLYAMGGSAGGLLVGAVINMRPDLFKGVIAAVPFVDVVTTMLDE
                     SIPLTTSEYDEWGNPTDSAYYNYMLSYSPYDNVAAMSYPALLVTTGLHDSQVQYWEPA
                     KWVAKLRVTKTDKNPLYLWTNMDYGHGGASGRFERYRETALEYAFLLDLEGVSE"
     gene            complement(3090..5402)
                     /locus_tag="PKM34_02595"
     CDS             complement(3090..5402)
                     /locus_tag="PKM34_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ82501.1"
                     /translation="MKRFLLLLLAIGGFTVAFSQTGTIKGFVFDKSNGEPVIFTNVYL
                     LKTTHGSATDENGYFIITKVPAGDYTLIVTYLGYDTLTMPISLQSGGVITKQLYLNPA
                     AINLETINISAEYQEARTETKTSVVKITPKDIGLIPSIGGQPDLAQYLQVMPGVVFTG
                     DQGGELYIRGGSPIQNKVLLDGVTIYKAFHSIGLFSVFETDIIRSADIYTGGFGAEYG
                     GRISSVMDITTRDGNRTRFAGKVGLSSFGANLLLEGPFVKIKENRSGSAGFILSAKGS
                     YLEQSSELLYKYIDTAGLPFNFLDLYGKVSIQASNGSKINFYGFHYRDQVNEYKTLSD
                     FNWHSTGIGTNFLVIPGKSDFIMEGHVAYSNYTIGLIEETSPERTSRISGFDVGLDFT
                     YFLGKDELKFGLEQVGSTTDYYFVNGSGMVIEQQENTTEIGLFAKYKLTAGKFLIEPS
                     FRLQWYASLSTVSPEPRLALKYNVTKHFRLKFAGGLYSQNLISARSDRDVVNLFYAIL
                     SGPDNLPDYFDGKPVTHALQKATHLILGGEFDIASQVTMNIEGYYKIFNQLTNLNRNK
                     IYDEDKNPEKPALLKEDFILEKGDAYGLDMTLKYDHRSLYFWVVYSYAFVNRYYEEID
                     TVMQQYYPHFDRRHNVNLVTTYRFGKQYGWEFSARWNYGSGFPFTQTQGYFEKIKFED
                     GIYSDYITDNGELAIIYGDLNKGRLPSYHRLDLSLKKRFFLSEHSKIDVDVSVVNVYN
                     RKNVFYRDRLTGEIVYQLPVMPSLGITWAF"
     gene            complement(5502..6422)
                     /locus_tag="PKM34_02600"
     CDS             complement(5502..6422)
                     /locus_tag="PKM34_02600"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNQ82502.1"
                     /translation="MKAKNIIWGLVLVLIGVMFILKNLDVIYFSWHSIWRLWPIVLVL
                     IGVTILPIKDLYKIILTVIVMIIGALFLIYVPGNRDNDWSFRFDRDITPPSPPDKLKD
                     IDQRIFEAYDTIITSAKLDFDAAAGNFRIEDTTAELFEFEREGNLGKYVYSIKDLGSR
                     REINIELDEGRIRGDFRNKVAVKLNPLPVWDLKVDVGAANIDMDLSSFKVENIDIDGG
                     ASSINVKMGSLYDHVNVKINSGASSINIKVPEEFGCKIVANTVLSSKDLNGFNHTGGG
                     NYETPDYQDKSKFITIKIEAAVSSLFIQRY"
     gene            complement(6426..6893)
                     /locus_tag="PKM34_02605"
     CDS             complement(6426..6893)
                     /locus_tag="PKM34_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNQ82503.1"
                     /translation="MQRKGLYRSRRDRILGGVAGGIAQSLNADPAIIRLIFAVLVIVG
                     GGGLLLYLILWIAIPEEPFEYYQSAFNPETSPGGQPAEGGETAAPSPVVYPARRNNGG
                     LIVGLVLICLGALFLLDRFLPTIHLHFHDFWPIIIVVAGITLIVSSFAGRKKS"
     gene            complement(7023..7829)
                     /locus_tag="PKM34_02610"
     CDS             complement(7023..7829)
                     /locus_tag="PKM34_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNQ82504.1"
                     /translation="MTKESAIKKGPEQAEQLSDEGIKRKIEDTLLALYQLQQHDSQID
                     KIRMIRGELPLEVQDLEDEIIGLQTRIENYHDEIKSFDKLIAEKKQAIKDSESLIRKY
                     EEQKMNVRNNREYDSITKEIEFQTLEMQLAEKRIKEYNEALNGKNAQISEAEGVLNER
                     RSDLEIKKSELNDIVEETENEEKTLAQRSEELQGRIDERLLTAYQRIRKNARNGLAVV
                     QIERDACGGCFNKIPPQHQLDIRMHKKIIVCEYCGRILVDSDITGMVKKN"
     gene            complement(7841..>8243)
                     /locus_tag="PKM34_02615"
     CDS             complement(7841..>8243)
                     /locus_tag="PKM34_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ82505.1"
                     /translation="ENEYDRAGAGMIGHLPEPTDEKVFLAQVKDILKIPVIRHSPLLG
                     KPLRRVAICGGSGSFLIQDAIRMQADIFLTADIKYHQFFEADGKIVIADAGHFETEQF
                     TCGLIAAYLKKKFPTFAIRISETLANPVNYF"
BASE COUNT         2159 a         2079 c         1780 g         2225 t
ORIGIN      
        1 acaagggaat cgggatcagc ccggagaacc agcgccatat tttcaggaac ctataccggg
       61 taccgacggg taacctccat aaagttaaag gatttgggat cggtttattt tatgtcaaaa
      121 cgattgtgga agcacacggc ggacatatta atttaacaag cgaactgaat aagggaagca
      181 gatttgattt atttttacct tttgtttata acttaaacaa ggaagatgaa tacacccaaa
      241 cctagcattc tcctggttga agatgatccg aatctgagcc tggttcttca ggattatctg
      301 gaaatgatga atttcgacat ccacctgtgc cgtaatggca aggaaggcct tgatgctttc
      361 cggcatagga aattcaatat ctgcattctc gatatcatga tgccggttat ggatggattt
      421 acccttgctg aagaaatcag aaagactgac gatacggttc cgattgtttt ccttactgcc
      481 aaatcgctga aagaagaccg catcaaggga ttcagaatgg gctgcgacga ctatattaca
      541 aaaccattca gcaccgaaga actgagtctc cgtatccagg ctattcttaa aagatgcatg
      601 aatcagccgg cccgggccga aaaagaagaa ccacccgtat acaatctggg gaattacatc
      661 ttcgaccaca aaaatctgat cctgagcatt aacggtgagc aaaaggtgct gacgcgtaag
      721 gagaatgccc ttctgaagtt actttttgaa catcagaacc agttgctccc gagagaagtt
      781 gcactaaaat cggtctgggg aaacgatgat tactttatcg gtagaagtat ggatgtcttt
      841 attactaagc tcagaaaata tttaaaagac gacccatcca tctttatcac caatgttcac
      901 ggaacaggct tcaaactgga agtaaaaaaa tagctactcc gagacgcctt ccaggtcgag
      961 caaaaaagca tactccagcg cggtttcccg gtatctttcg aaccgcccgg aagccccgcc
     1021 gtggccgtaa tccatattgg tccataaata aagcgggttc ttatcggtct tggttaccct
     1081 gagtttagcc acccactttg ccggttccca gtactgaacc tgcgagtcgt gcaaccctgt
     1141 cgtgacaagc aatgcaggat agctcatcgc cgcaacatta tcatacggcg aataggagag
     1201 catatagtta taataagcag aatcagtggg attcccccac tcatcgtact cactggtagt
     1261 caatgggatg ctctcatcca gcatggtcgt aaccacatca acaaatggaa ctgccgcgat
     1321 tacacccttg aatagatcgg gccgcatatt gatcaccgcg ccaaccagca ggccgccggc
     1381 actaccgccc atagcatata gctgccctgg attcgtatat ccacgattga ccaggtattc
     1441 gccgcaggct ataaaatcgg tgaacgtatt cttcttgttc atcaatttgc catcttcata
     1501 ccacatccgg cccatctcct ccccgccccg gatatgggca atagcgtaga taaaacccct
     1561 gtcaagcaaa ctgagcctca cggaattaaa ataaggatcg gtcgaataac cgtatgaacc
     1621 gtaaccgtat agaaggaccg gaacatgccc gtcccgcttc aaatctttcc ggtaaaccat
     1681 cgacagggga atctgttttc catcggatgc agtgacccac acgcgttcgg taaaataatc
     1741 cccgggttcg taaccgccaa ccacctcctg ccgctttttc atttcgctct cacgggtcct
     1801 cgtattataa tcaaaaaccg tcatgggagt tgtcaaggag gtgtaattgt acctgaccac
     1861 ctccgtctcc atctcctcat tgctgcccag ggtgaccgta taagcctgct ctgtgaaatc
     1921 caggtaataa tccagtccgg cgtggttgaa aatacggatg gtcttcaatc cctctttgcg
     1981 ctcctggatc gccagaaatc ctttgaacac atcaaaatcc tccagcaaga catcctcccg
     2041 gtgcccgatc acctctttcc agttctccct gccggtcgcc ccttccggag tctccatcag
     2101 cctgaagttt accgcattat cattggtccg gatgtaaaac ctgtcttcat aatgcgcaac
     2161 tgaatactca agccccctga tcctcggctg aaacaccgta aaccgtccat tcggatcacc
     2221 ggcatccatg taacggtatt catccgacat cgtgctccct gatccgatca tcaggtattt
     2281 cctggattta ctcctgtaga caaaggtgtt atacgtaaca tcttcttcaa aataaaccag
     2341 ttcgctttct ttttctgatt gtccgagcct gtatttccag atcttatcgg gccgcagtga
     2401 ttcatcccgc gttgaataaa acaaggtctt gttatcggcg gcccaggcca ggttgcctga
     2461 tgtctggccg atccggtcag gatagatctc accggtcccc aggtttttaa agtacagggt
     2521 gtagatccgg cggctcacag tatcaaccga aaaagcaagc aggcggttgt cgtcgctgac
     2581 ttccagtccg cccacatcat aataagcgta cccggcagcc atctcattga cattcagcat
     2641 gatctcctca acactgtcca ggcttccttt ttttctgcaa tataaaggat attctttccc
     2701 ctcttcgtac cgggtatagt aataataacc gttttccacg taaggcaccg acatgtccgt
     2761 ctgcttgatc cttcccgtta tctccgtgaa caatttctcc cggagcccat cggtatgagc
     2821 catcatagct ttggtatact tattctcagc ctccaggtag gcaaccacat ccggattttc
     2881 acgttgattc agccagtaat aattgtcgat ccgtgtctga ccatgaatgg tcagctcctt
     2941 tatctccttt tttgcaatcg gtgcgtccat cgtttgctgt tttttttgtt cactgcaacc
     3001 ggaaatcaaa aggcataccg gcataatcca taataatcct ttgaataaat tgtggtccat
     3061 gttttcaata ttttaaccgg gaaatatgtt taaaatgccc atgtaatgcc gaggctgggc
     3121 atgaccggca actggtatac gatctcgccg gtgaggcggt cgcggtaaaa gacatttttc
     3181 cggttgtata cattgactac gctgacatcg acatcgatct tggaatgctc gctcaggaaa
     3241 aacctttttt tcaggctcag gtccagacga tgatacgaag gcagcctgcc tttgttcagg
     3301 tctccgtaaa taatggccag ttcaccgtta tcggtaatat agtcggaata tatcccatcc
     3361 tcgaatttaa tcttctcaaa atagccctgg gtctgggtaa acgggaaacc tgaaccgtaa
     3421 ttccaccgtg cactgaactc ccatccgtat tgttttccga aacggtatgt cgttaccagg
     3481 tttacattat gacgccggtc aaaatgagga taatattgct gcatcaccgt atcgatctct
     3541 tcatagtaac gattgacaaa tgcgtaagaa taaactaccc agaaatagag actgcggtgg
     3601 tcatatttga gcgtcatatc taatccgtat gcgtcgccct tctccaggat aaagtcctct
     3661 tttaataatg ccggtttttc aggattcttg tcttcatcgt agatcttatt ccggttcagg
     3721 tttgtcagct ggttgaagat cttgtaatat ccttctatat tcatcgtcac ttgtgaggca
     3781 atgtcaaact ccccaccaag gatgagatgg gtggcttttt gcagggcatg ggtcaccggt
     3841 tttccgtcga aataatcggg aaggttatcg ggaccggaaa ggatggcata gaaaaggttg
     3901 acgacatccc tgtcagaccg cgcactgatc aggttttggg aatacaggcc cccggcgaac
     3961 ttgagcctga aatgtttggt cacattatat ttcagtgcaa ggcgaggctc cggcgaaaca
     4021 gtcgacaaag aggcatacca ttgaagccgg aagctgggtt ctatgaggaa tttcccggcc
     4081 gtcagcttgt acttggcaaa caatcctatt tcagtggtgt tctcctgttg ttcaattacc
     4141 atccccgatc cgttcacaaa gtaataatct gtggtggaac cgacctgttc caggccgaac
     4201 ttgagttcat cctttccaag gaaataggta aaatcgaggc cgacatcaaa cccggagatc
     4261 ctgctggtcc gctccgggct ggtctcttca atcaggccga tggtgtaatt ggaataggct
     4321 acatgcccct ccatgataaa atcagacttg cccgggatca ccaggaaatt ggtgccgatt
     4381 cctgtgctgt gccagttgaa atcagaaagg gtcttgtatt cattcacctg gtcacggtaa
     4441 tgaaacccgt aaaaattgat tttgctgccg ttgctcgcct ggatcgaaac tttaccgtaa
     4501 agatccagga agttgaaagg caatccggcc gtatcgatat acttgtataa taattcggag
     4561 ctttgctcca ggtaagaacc tttggccgat aaaataaaac ctgcgctccc gctcctgttc
     4621 tctttgattt taacaaacgg cccctccaga aggaggttcg cgccgaaaga acttaaaccc
     4681 acctttccgg caaagcgcgt gcggtttcca tccctcgtcg taatgtccat gaccgaagag
     4741 atccgtccgc cgtattctgc gccgaaacct ccagtgtaaa tgtcggcact gcggatgata
     4801 tcagtctcaa aaacactgaa caggccaata gagtgaaatg ctttatatat cgtgaccccg
     4861 tcgagcaata ctttgttctg gatcggggaa ccgccccgga tatacaattc accgccctgg
     4921 tcacccgtaa aaacaacccc cggcatcacc tgcaggtact gggccaggtc aggctgcccg
     4981 ccaatggatg gaatcaaccc gatgtccttt ggcgtaattt taaccacaga agtttttgtc
     5041 tcagtgcggg cttcctggta ttcggctgaa atattgatgg tttcaaggtt aatggctgca
     5101 gggttcaaat agagctgctt tgtaatgacc ccgcccgatt gaaggctgat cggcatcgtc
     5161 agggtatcat agcccaggta ggtgacaatc agggtgtaat ctcctgcagg cactttggta
     5221 attataaaat aaccgttttc atctgtcgca gatccatgag tggtttttaa aaggtaaaca
     5281 ttggtgaaga tgaccggctc accgtttgat ttgtcaaaaa caaacccttt gatggttccg
     5341 gtctgactga acgctacagt aaaaccgcca atggccagta agagaagaag aaaccttttc
     5401 atcagactct gggttgaaaa tttgcaaaaa cggcaaattt accattattc ccaaatgccg
     5461 ggctgaaaaa tcagcgaacg gtagaattta ataagcctgt ctcagtatcg ttgaataaac
     5521 aggcttgaaa cggctgcctc gattttaatc gtgataaatt tgcttttgtc ctgataatcg
     5581 ggtgtctcgt agttgccgcc cccggtatgg ttgaaaccat taaggtcttt tgaagatagt
     5641 actgtattgg caactatctt gcagccaaac tcttccggaa ctttaatatt gatcgaagag
     5701 gcacccgaat ttatcttgac gttcacatga tcgtaaagcg aacccatctt cacattgatc
     5761 gacgacgccc cgccgtcaat atcgatattt tccaccttga acgacgaaag atccatatcg
     5821 atattggctg cccccacatc gactttcagg tcccaaacag gcaacggatt gagcttaact
     5881 gcgaccttgt ttctaaaatc ccccctgatc cggccttcat ccagctcaat gttgatctcc
     5941 cggcggctgc caaggtcttt aatggaataa acatatttac ccaggtttcc ttcccgctcg
     6001 aattcaaaca actcggcagt agtatcctca atacggaaat ttccggcggc cgcatcgaaa
     6061 tccaattttg ccgaggttat gatcgtatca tacgcttcaa agatccgctg atcaatgtcc
     6121 tttaacttgt caggcgggct gggaggggtt atatcccggt caaaccggaa cgaccagtcg
     6181 ttatcccggt tacccggaac atagatcagg aacaatgccc ctatgatcat gacgatcacg
     6241 gtgagtataa ttttataaag atctttgatc ggaagtatgg ttacgccaat aagcacaaga
     6301 acaatgggcc agaggcgcca gatgctgtgc cagctgaaat aaatcacatc gagattcttc
     6361 agtataaaca tgacccctat cagaaccagg accagtcccc agataatatt ctttgctttc
     6421 atggatcagg atttttttct tccggcaaaa ctactgacaa taagtgtgat accggccact
     6481 acgatgatga tgggccagaa atcatgaaaa tgaaggtgaa ttgtaggtaa aaacctgtcg
     6541 agaaggaaca gggcaccgag gcagatcagc accaggccga cgataaggcc gccgttgttc
     6601 cggcgcgcag gataaaccac cggcgaaggt gcagcagttt cacctccttc agcaggctgg
     6661 ccaccgggtg aggtttccgg attaaatgcg gattgataat actcgaaagg ttcttcggga
     6721 atcgcgatcc agaggatcag gtagagcagc agcccgcccc ctccgacaat taccaggacg
     6781 gcaaaaatga gcctgataat ggcaggatct gcattcagcg attgggcaat gccgccggct
     6841 acaccaccca gaatacggtc acggcggctg cggtacaatc cttttctttg catgtgattt
     6901 aaatttgtta taaatgtata aacctaagac gaggttttca atttcatgtt acaaatataa
     6961 caaaataaaa agggtgtctc aaaagtcaga gcaccctctt tataaacaga atttcaataa
     7021 gctcagttct tttttaccat gccggtaata tccgaatcga ccaggatccg gccgcaatat
     7081 tcgcatacaa tgattttctt gtgcatccgg atatccagct gatgctgggg aggaatcttg
     7141 ttgaaacacc caccgcaggc atcccgctcg atctggacca cggctagccc gttgcgggca
     7201 ttcttccgga tccgctggta agccgtcagt aacctctcgt caatccttcc ctgcaattct
     7261 tccgatctct gtgccagtgt cttttcttca ttctccgtct cctccacaat gtcgttcagc
     7321 tcgctctttt ttatctccag atcggaacgc ctctcgttca atacaccttc tgcttcgctt
     7381 atctgtgcgt tttttccgtt cagggcttca ttgtattctt tgatcctctt ctcagccagt
     7441 tgcatctcaa gcgtttgaaa ctcaatttct tttgtgatcg aatcgtattc cctgttgttc
     7501 cttacattca tcttttgctc ttcatatttg cggatcagcg attcactgtc tttgatggcc
     7561 tgctttttct cggcaatcaa cttatcaaac gattttatct cgtcatgata attttcgatc
     7621 cgggtctgta aaccgatgat ttcatcctcc aggtcctgca cttccagagg cagttcccca
     7681 cggatcatcc gaattttatc gatctgcgaa tcatgctgct gaagctggta taacgctaat
     7741 agcgtatctt cgatttttct tttgatccct tcatctgaaa gctgttcggc ctgttcagga
     7801 cctttcttaa tcgcactttc ctttgtcata aattattgat ttaaaaataa ttaaccggat
     7861 tagccagggt ttctgaaatt cggattgcaa aggtaggaaa ttttttcttc aaataagcag
     7921 cgattaaccc gcaagtaaat tgttcagttt caaaatgtcc tgcatcagca atgacgattt
     7981 tcccgtcggc ttcaaaaaac tggtggtatt ttatgtcggc cgttagaaaa atatcggcct
     8041 gcatccggat ggcatcctgg atcagaaaac tcccggagcc gccacaaata gccacacgcc
     8101 tcaaaggctt tcccaataaa ggagaatgac gtatcaccgg aatcttcagg atatctttca
     8161 cctgtgccag aaaaaccttt tcatccgtag gttcaggcaa atggccgatc atccctgcac
     8221 ctgcccggtc atactcgttt tcc
//
LOCUS       DAOQXS010000052         8225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9184_length_8225_cov_3.333048, whole genome shotgun sequence.
ACCESSION   DAOQXS010000052 DAOQXS010000000
VERSION     DAOQXS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_8225_cov_3.333048"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKM34_02620"
     CDS             <1..250
                     /locus_tag="PKM34_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82506.1"
                     /translation="ILQVSSVDQTRSETYILGKKTEPAVKTGDQFQAGDTLYTGKVVN
                     RILYTDLARLLLLSLFLFSSVLVYLAAVKRKKYADRHH"
     gene            262..414
                     /locus_tag="PKM34_02625"
     CDS             262..414
                     /locus_tag="PKM34_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82507.1"
                     /translation="MNTSAIIFMALAQTIITLITFSFLYRAMRAKKRPEPDSYADNDD
                     DDDDEN"
     gene            533..1489
                     /locus_tag="PKM34_02630"
     CDS             533..1489
                     /locus_tag="PKM34_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNQ82508.1"
                     /translation="MINRIKDYFSFTAKEQRGLIALLGIILLALSAGIYLPVLIPQKR
                     FDIEPFRSEVEAFLAKAVIEDSSAISNQNQALKFTPVSDHQDLEGFQQSPYPFNPNEL
                     DESQWKKMGLRDRIVNNIMNYRKKGGRFRDKEGFRKIYGLSDKEFGILEPYLVFDPPV
                     KSEYPKDTASKPDGFHDQYTKKDFLPIRVEINSADSAALLNLKGIGPSFAGRIVKYRS
                     RLGGFYKPEQLMEIRGLDSVRFNQFRDQIELNSGLISKIDINNVTFKELLKHPYFEYY
                     LVKAIFEKKDQIKSFDSVEQIKYLPVMYEELFEKISPYITVN"
     gene            complement(1482..2264)
                     /locus_tag="PKM34_02635"
     CDS             complement(1482..2264)
                     /locus_tag="PKM34_02635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNQ82509.1"
                     /translation="MIRQEERKILRSMVIPFMLVLLMWAVKIIEVTGDYKLSFLGIFP
                     LRWQNLTGIITTPFIHGDFSHLIANSVPLIILGSALFYFYKDLSFRILLFIWLFSGFW
                     VWIAARPAFHIGASGIVYGLATFIALSGIIRKHTGLMALALIVVFLYGSLIWGIFPEF
                     FPEKNISWEAHLYGMIAGIILAVYYRKQGPQRKVYEWELEEEEETGRHGDQETRRHGD
                     QETGRHGDQETGRQGDDFWNVSITDEEIDKIRHIYRPNKNVN"
     gene            complement(2337..3335)
                     /locus_tag="PKM34_02640"
     CDS             complement(2337..3335)
                     /locus_tag="PKM34_02640"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNQ82510.1"
                     /translation="MGLTTGIQGQSARIVFYNVENLFDVMDDTLTNDEEFLPEGDKNW
                     TYDRFREKTIKIYRTLVALGQGDMPAVIGLCEVENRLVLNRLIYDTPLSLKDYRLIHR
                     ESHDARGVDVALIYRADLFFPITSEWLTIPLRSGETTREILHVKGRIWDRDTLDIYIN
                     HWPSRYGGAGTSVQKRMAAAAILSESIVKVLKSNYNANIIVMGDFNDEPDDESMQSVN
                     KILENSLQDSFCPLVNLSSLEKNIFGRGTIKHQGVWSVFDQILVSCALFSGDSGLRVS
                     DQMAGIFMAEFLLEPDNAYSGMKPFRTYLGPGYHGGFSDHMPVFLDVEKTGTLKRK"
     gene            3503..5881
                     /locus_tag="PKM34_02645"
     CDS             3503..5881
                     /locus_tag="PKM34_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ82511.1"
                     /translation="MTKAHFSQISSLLTGIIFLTCLFFNATAGIDPDDPSVKRYTISG
                     HIKDNTSGEDLFGSSVLVTEQNTGTIANEYGFYSVSLTKGIYTLRFSYMGYETQTHKI
                     TADKDIVLDINLNPSSAILNEVEIRAERGDANIKTPEMSVVKLDVKTINRIPALMGEV
                     DIIKAIQLLPGVQSVSEGSSGFSVRGGSPDQNLVLLDEATVYNPSHFLGFFSVFNNDA
                     IKDVKLYKGDIPAQYGGRLASVLDVRMKDGNQKKFSGTGGIGLISSRLTLEGPIIKDK
                     TSFILSGRRTYADLFLPLSKNEGVQDSRLYFYDFNAKINHTFNENNRLYLSGYFGNDI
                     FKNPFAYMKLGNTTATIRWNHLFSKRLFSNFTAIYSRYQYGLGTADDDNPNSFLWTSV
                     LQDYQVKGDFTYYPGTNHTLRFGFTSSLHLLDPGSASGRGDSSLFTEYSIDRNHSLES
                     GLYVSNDQLIGSRLTLKYGIRFSLFQNIGPSTVFLYDDEFHLSDSTKYEHGKFYNTYT
                     GLEPRLGLTYVLDEFSSVKASYTHTYQYLQLAQNSTAGTPLDVWYSASPNVKPQISDQ
                     AAIGYFRNFRKNTIETSVEVYYKTMRNTIDFKDHAELLLNKQLEGELRFGKGWSYGVE
                     LMVSKPQGKITGWVSYTFSRTWRKFEDINDGKKYPAPYDSPHDVSIVLNYDATKRISI
                     SANWIYSTGKPVTFPTGRAVIDGVVVPIYSDRNAYRMMDYHRLDLSVALHKKPSDSRF
                     DWDLVFSVYNAYNRHNTWAINFVQDNLYPLTTYAEQTYLFGIIPAVTFNFQF"
     gene            5881..6729
                     /locus_tag="PKM34_02650"
     CDS             5881..6729
                     /locus_tag="PKM34_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNQ82512.1"
                     /translation="MMKRIHILYLLIPVILSSCTERIDIDLDETYTRLVVYGVLTTDT
                     TTHYVTLSKTTSYFYNELPPPVSGAEIIISDDQGNEFTLQETEPGKYATSPLFAAVQG
                     RTYTLNISLEEEINNFRNYTASTKVTSINPIDSIGLVHQPLWGEKGFYEVTCYYQEPP
                     TRDFYMFNILINGKLVTDTINSRIVINDDFFNGNYTNGIGVGYLDQSKTDEILRPGDE
                     VTFQGCNITEEFYDFVMTLQSETGFQTPLFSGPPANIKSNLSNGAVGFFTAYSVAYSS
                     KVFQLQ"
     gene            7076..7585
                     /locus_tag="PKM34_02655"
     CDS             7076..7585
                     /locus_tag="PKM34_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82513.1"
                     /translation="MKKLILTFIILNSVILSSQSQDKLKIGDVRNDKLVVSNVEGLKK
                     FLYNSIGNSGTLGKDLQYRLAPEGDRCVVFYPVSGNRENVTSAGVLLIVKNGEAFIQE
                     SPEIIDSSGPGGGGSYEIQCVGTNCNQCVPAIKWIGNWLPYVICECKQEGDGTCNMIT
                     KLIVNVQFN"
     gene            7731..>8225
                     /locus_tag="PKM34_02660"
     CDS             7731..>8225
                     /locus_tag="PKM34_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82514.1"
                     /translation="MFRIIRILVLLLAFTNLMAQEKSSAIQNDDPFIKMVRATDALSA
                     FIFTSQLNMKQVFTFDTIRNTAEIRVKKSGAKILFLQIIPSESQKELLFFLDSAWVVD
                     HTIRNMALIGHTHDDLSHNFISTSYPFSLFTLDTMLAYVSPFWKITGENNDFYEVTMD
                     IANKS"
BASE COUNT         2377 a         1896 c         1710 g         2242 t
ORIGIN      
        1 cattttgcag gtttcttcag tcgatcaaac caggtcggag acctacatac tcggcaagaa
       61 aactgaaccg gcagttaaaa cgggcgatca gttccaggcc ggcgatactt tatatacagg
      121 taaagtggtg aacaggatcc tgtataccga cctggcgcga ctcctcctgc tgtcgctctt
      181 tcttttctcg tccgtattgg tttacctggc tgccgtaaaa cgaaaaaaat atgccgatcg
      241 tcaccattaa cacgtaaaac tatgaatacc tcagcaatta tatttatggc gctggcacaa
      301 acgatcatta ctttgatcac cttctcattc ctgtaccggg caatgagagc taaaaaacgc
      361 cctgagcctg attcttatgc agacaatgat gacgatgatg atgacgagaa ttaaattcgt
      421 cagtcttaaa gatcacagag tatcatcctt cgtaaactat tgtatataag ttacttgtaa
      481 ttttcttctt caccgaccgg gtatttttta ccctttttcc ggattaatca gtatgataaa
      541 ccggatcaaa gattactttt catttactgc gaaagaacag cggggcctta tcgccttgct
      601 ggggattatc ctgttggccc tgtcagccgg gatttacctg ccggtactaa ttcctcaaaa
      661 acgatttgat attgaaccct ttagaagcga agtcgaagcg tttcttgcaa aggctgtgat
      721 cgaagactcc tcagccattt caaaccaaaa tcaagcttta aaattcactc cggtatcgga
      781 tcatcaggac cttgaaggtt ttcagcagtc accttacccg ttcaatccta atgagctcga
      841 tgaaagccaa tggaagaaaa tgggtttgag ggatagaatt gttaacaata tcatgaacta
      901 caggaagaaa ggtggccggt tcagggataa ggagggattc aggaaaattt atggtctgtc
      961 agataaagaa ttcggtattc tcgaacctta cctagttttt gatcctccgg taaaatctga
     1021 atacccaaaa gacactgctt caaagcctga cggatttcac gatcaatata caaagaaaga
     1081 tttcctgccc atcagggtgg agatcaacag tgcagattcg gctgctttgc tgaacctgaa
     1141 aggaattgga ccctcttttg ccggcaggat cgtaaaatac cgctccagac tgggaggttt
     1201 ttataagccg gaacaactga tggaaataag aggtctcgac agcgtccgat ttaaccaatt
     1261 ccgtgaccag atcgagttaa actctggttt gatctcaaag attgatatta ataatgtaac
     1321 tttcaaagag ttactaaagc atccatactt tgagtactac ctggttaaag ccatttttga
     1381 gaaaaaagat cagattaaat catttgattc agtagaacaa ataaaatacc tgccggtgat
     1441 gtatgaagag ctctttgaga agatatcgcc ttatataaca gtcaattaac atttttattc
     1501 ggacgataaa tatgcctgat cttatcgatt tcttcatcag tgatggaaac attccaaaaa
     1561 tcatctcctt gtctcccggt ctcctggtct ccgtgtctcc cggtctcctg gtctccgtgt
     1621 ctcctggtct cctggtctcc gtgtctcccg gtctcctctt cctcctccag ttcccattcg
     1681 taaactttcc gttgaggccc ctgttttcta tagtaaacag ccaggataat accggcaatc
     1741 attccataaa gatgagcttc ccaggagata ttcttttccg ggaagaattc gggaaaaatc
     1801 ccccagatca ggcttccata aagaaaaaca acgatcaggg cgagagccat caaaccggta
     1861 tgcttccgga taatcccgct cagggcaata aaagtggcaa ggccatagac aatcccgctg
     1921 gccccgatat gaaaagccgg ccgggcagct atccagaccc aaaatccact gaatagccag
     1981 ataaacagta atattctaaa tgacaggtct ttgtaaaaat aaaataacgc cgatcccagg
     2041 atgatcaatg gtactgaatt ggcaataaga tgggaaaaat cgccgtgtat gaaaggtgtt
     2101 gtgatgatac cggtgagatt ctgccaccgt aaagggaata tccccagaaa ggacaatttg
     2161 taatctcccg taacctcgat gattttaaca gcccacatca gcaaaaccag catgaaagga
     2221 atcaccatgc ttctcagtat ttttcgctcc tcctgtctga tcatatctga aaatttccgg
     2281 ttgtgccagc acaaatcagg ccacttgact tggctaagtt agttatttga tggatttcat
     2341 tttcttttta aggtgccggt tttttcaaca tccaggaaaa caggcatgtg atcactgaat
     2401 ccgccatggt aacccggccc aaggtaggtc cggaatggtt tcattcccga ataggcatta
     2461 tccggttcaa gcagaaattc agccataaag atgcctgcca tttggtcaga gaccctcaaa
     2521 ccggaatcac ccgaaaagag tgcgcatgaa accaggatct gatcgaaaac cgaccaaacc
     2581 ccctggtgct tgatcgtgcc tctaccaaaa atgttctttt ccaggctact caggtttacc
     2641 agcgggcaaa atgaatcctg cagactgttt tccaggattt tgttgaccga ctgcatgctt
     2701 tcatcatccg gttcatcatt aaaatcaccc atgacaatta tgtttgcatt ataattgctt
     2761 ttaaggacct ttacgatcga ttcggacaaa atggccgccg ctgccatcct tttttgcact
     2821 gaagttccgg ccccgccgta tcttgacggc cagtggttaa tataaatatc cagcgtatcc
     2881 ctgtcccaga tccggccctt tacgtggagt atctcccttg tcgtttcacc cgatctcaaa
     2941 ggtatggtca gccattctga agtgatcggg aaaaacaggt cagcccggta gatcaaagcc
     3001 acatccacac ccctggcatc atgcgactct ctgtgtatca accggtaatc cttaaggctt
     3061 aggggtgtat cataaataag cctgttcagc acaagcctgt tttccacctc acataatcca
     3121 ataacggcgg gcatatcccc ttgccctaat gcaacaagag tcctgtaaat cttaatggtc
     3181 ttttcccgga acctgtcata agtccagttt ttatcccctt ccggcaaaaa ttcttcatcg
     3241 tttgtcaaag tatcatccat gacatcaaat aaattttcaa cgttataaaa gacaatacgt
     3301 gccgattgtc cctggattcc ggttgttaat cccatcagga tcaaacaaac tcaattacaa
     3361 aataaacttt tcatctgatt tcctttttcc tattaatccg gtttttggga tgaaaatacc
     3421 cggtcggtga aaaaaatatt cggaaagtgc aactatttgg ccttttattt gtcattatcc
     3481 tgatcttttg aatcatcata aaatgacaaa ggcacatttc agccaaattt caagcttact
     3541 aacaggcatt atctttctga cctgcctttt ttttaatgcc actgccggca ttgatccgga
     3601 cgatccatcc gtgaaacggt atacgatcag cgggcatatc aaggacaata ccagcgggga
     3661 ggaccttttc ggctcttctg tcctggtcac cgaacaaaat accggcacga ttgccaatga
     3721 atatggattc tactcggtca gcctgaccaa agggatatat acgcttcgct tttcatatat
     3781 gggatatgag acccagacac acaagatcac tgccgacaaa gacatagtcc tggatattaa
     3841 tctcaatcca tcgtctgcta tattgaatga agtggaaatc agggctgaac gcggcgatgc
     3901 caatataaaa acgccggaga tgagcgtagt taagctggat gtcaaaacga tcaaccggat
     3961 tccggcactg atgggcgaag tggatattat caaagcgata cagcttcttc ccggggtaca
     4021 atcggtgagt gaaggttctt cgggttttag tgtaagagga ggaagccccg accagaacct
     4081 ggtattgctg gatgaggcta cggtttataa tccctcccat tttctcggat ttttttcagt
     4141 attcaataat gatgcgataa aagatgttaa actatataag ggcgacatac ccgcacaata
     4201 cggaggaagg ctggcttcgg tcctggatgt gcgaatgaaa gacggaaacc agaagaagtt
     4261 ttccggaacc ggagggatag ggcttatttc cagcaggtta acgcttgaag ggccgataat
     4321 taaagataaa acctcattta tcctgtcggg tcgaagaact tatgccgatc tctttttgcc
     4381 cttgtcaaaa aatgaaggag ttcaggacag ccggctctat ttttatgatt ttaatgccaa
     4441 aatcaatcat acttttaatg agaacaaccg gttatatcta tccggttatt tcgggaatga
     4501 catctttaag aatccttttg cctatatgaa actggggaac acaacggcta ccatccgttg
     4561 gaatcacctc ttttcaaaaa ggctcttcag caatttcact gcaatatact cccgctacca
     4621 gtatggttta ggaacggctg atgacgacaa tccgaactca ttcctttgga cttctgtttt
     4681 acaggattac caggtcaaag gagattttac atattatccg ggaacaaacc atacgcttcg
     4741 cttcggattc accagcagcc tccatttgct cgatccggga agtgccagcg gaagaggcga
     4801 ctcaagcctt ttcacggagt attcaatcga ccgtaaccat tccctggaga gtgggttata
     4861 cgtaagcaac gaccagttga tcggcagccg gctgactttg aaatacggca tcaggttttc
     4921 tctctttcag aacattggtc catcaaccgt ttttctatat gatgatgaat ttcatctgag
     4981 cgattcaacg aaatatgaac atggaaagtt ctataataca tataccggcc tggaaccccg
     5041 tcttgggctg acatatgtcc tggacgaatt ttcatcggta aaggccagct atacgcatac
     5101 atatcagtat ttgcaattag cacagaattc aactgcgggt acgccgctcg atgtctggta
     5161 ttccgccagt ccgaatgtaa agccgcagat ctccgaccag gctgcaattg gttatttccg
     5221 gaacttccgc aaaaacacta ttgaaacttc ggtagaggta tattataaga ccatgcgcaa
     5281 tacgatcgat tttaaagatc acgctgaact tttactaaac aaacaactgg aaggggaact
     5341 gcggttcgga aaagggtggt cgtacggagt agaactgatg gtctccaaac cgcaaggcaa
     5401 aatcaccggt tgggtaagtt acacgttctc acgcacctgg agaaagtttg aagacattaa
     5461 tgacgggaag aaatatccgg ccccgtacga ttccccacac gatgtatcca tcgtcctgaa
     5521 ttatgatgcg acaaaaagga tcagcatatc agccaactgg atatattcta ccggcaaacc
     5581 ggtaactttc ccaacaggaa gagccgtgat cgatggcgtg gtagtgccga tttattctga
     5641 tagaaatgcc taccgcatga tggattatca ccgtcttgac ctttcggtag cactgcataa
     5701 aaagccgtct gattcacgtt tcgactggga tctggtattt tcggtctata acgcgtataa
     5761 ccggcataat acatgggcca tcaattttgt tcaggataac ctgtatcctt tgacaaccta
     5821 tgcagagcaa acgtatttat tcgggatcat tccggctgta accttcaact ttcaattctg
     5881 atgatgaaaa gaatccatat cctctatctt ctgatcccgg tgattttatc ctcctgtaca
     5941 gagcggattg atattgacct ggatgaaacc tatacccgcc tggttgtgta tggcgtactc
     6001 acaaccgaca caacaacgca ctatgtaaca ttatcaaaaa caacaagcta cttttataac
     6061 gaacttcctc ccccggtttc aggagcagaa ataattatca gcgatgacca gggaaatgaa
     6121 ttcacccttc aggaaaccga accgggaaag tatgccactt cacctttatt tgccgctgtg
     6181 cagggccgga cctatacctt aaacatcagc cttgaagaag aaatcaataa tttcagaaat
     6241 tataccgcct caacaaaagt cacatccatc aatccgatcg actcgatcgg gctggtgcat
     6301 caacctttat ggggcgaaaa aggattttat gaggtgacct gctattacca ggaaccaccc
     6361 acacgcgact tttatatgtt caatatcctc ataaacggaa aactggtgac cgataccatc
     6421 aacagtagga ttgtcattaa tgatgatttc tttaatggga attataccaa cgggatcggt
     6481 gtgggatacc tcgaccagtc aaaaacggat gaaattttac ggcccggcga cgaggtgacc
     6541 ttccagggat gcaatataac ggaggagttc tacgattttg tcatgaccct gcaatcagaa
     6601 acggggtttc agaccccgtt gttcagtggt cctccggcaa acattaaaag taacctctca
     6661 aatggcgcag tcggcttttt tacggcctac agcgttgctt actccagcaa ggttttccag
     6721 ctgcaataaa aaaatatttt cattcgaccg ggtatttttt actcaaaatc cggattaaca
     6781 ggtagggaag attgttacct gttttatccg ggctggctcc cctgacaaaa cttttatcag
     6841 gcaacaaggg attctcttcc tgttactaaa atcttacaag gccagatcca attaatacta
     6901 aacctctgcc acagctcagt gttttctaaa tactaccgct gctggcggcg ccggttcttc
     6961 ctttgatgca gtgcgtagaa atttgcaggg ctgaggtagt tcgactgcga aagtggaata
     7021 gtgcccggtt gccgaaaagg gctgtcagag taggcaatac tttaattcat cggttatgaa
     7081 aaaacttatt ttaacattca ttattctgaa ttccgtaatc ctctcttctc aatcacagga
     7141 caagctgaag atcggagatg taagaaacga taagctcgtg gtttccaacg ttgaaggatt
     7201 gaagaagttc ctgtacaaca gtatcgggaa tagcggaact ctgggaaaag acctccagta
     7261 caggcttgct cctgaaggcg accgctgtgt ggttttctac cctgtttccg gaaacaggga
     7321 gaatgtgacc tcagccgggg ttttgctgat cgtgaagaat ggcgaagcat ttatccagga
     7381 aagccctgaa ataattgatt cttcgggacc cggcggcggt ggaagttatg aaatccagtg
     7441 cgttggtaca aactgcaatc aatgcgtacc tgcaatcaaa tggataggaa attggctgcc
     7501 ttatgtaatc tgtgaatgca agcaggaagg cgacggtacc tgtaatatga ttacgaaatt
     7561 aattgtcaac gtgcaattca actaaagaat gatgatttga ctttcccgga tggcgaattt
     7621 ccactattcc gaatatcacc ttccggggaa gtctttaaac cgaatcgaag aaataaattt
     7681 aaataagtta aatataaatt taattgtctc accattaacc ctttaaaacc atgtttagaa
     7741 ttatccgcat ccttgtttta ctcctggctt tcacgaattt aatggcacag gaaaaatcat
     7801 cggccattca gaatgatgat cctttcatca aaatggtcag ggcaaccgac gcattaagtg
     7861 cgtttatatt cacatcgcaa ctcaatatga aacaggtctt tactttcgac acgattcgca
     7921 atacggcgga gataagagtt aaaaaatcgg gcgctaaaat cctgtttctg caaattattc
     7981 cctctgaaag tcagaaagag ctccttttct tcctcgactc agcctgggtg gtcgatcaca
     8041 caatcaggaa tatggcccta atcggacata cacatgatga tctgtcccac aatttcatct
     8101 caacatccta cccgttttcc ctttttaccc tggacaccat gcttgcttac gtctcccctt
     8161 tctggaagat taccggcgaa aacaatgatt tctatgaagt taccatggat attgccaaca
     8221 aatcc
//
LOCUS       DAOQXS010000053         8198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9226_length_8198_cov_4.076016, whole genome shotgun sequence.
ACCESSION   DAOQXS010000053 DAOQXS010000000
VERSION     DAOQXS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9226_length_8198_cov_4.076016"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2548
                     /locus_tag="PKM34_02665"
     CDS             <1..2548
                     /locus_tag="PKM34_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNQ82515.1"
                     /translation="GSMPTIRIRGIGSFPGRENEAPLFVVDGMFFDNIDFLNPSDIAS
                     ISVMKDASAAAIYGVRAANGVVLIETKSGQYNQKAEITYNGYGGYQIAQNVVKMANSQ
                     QFSIMARESGSPADETFILNAMQRYGRSRVDPSIPEPNTNWYAEILRPAAITNHSLDF
                     SGGTEKARYSLGANYFYQDGIMNMDNNYERLNLRTKLDFKATNWLTVGGNMVYSNALK
                     NNEQNSAWNNAYFAVPIMPVYDTLNDLAYPDNYSSARIMGYRSGQNPFPYMEYNFDQM
                     KIKKLLSNFYVEINLLPKVLTFKTSYNYSYTGIDQRIINFPYYIDDGFNNLDPKITRT
                     WTNYNNHIWDNILTFTKSFQNHNLTLMAGTSFKDEGYTMLSAWVNDIPTDKPQSWYIN
                     KTLDIESIGATDGGLRQYGMSYFGRLAYNFNNRYLLYATMRADGSNKYQQTWGYFPTV
                     GIGWVVTEEKFMPKNDILTYLKIRASWGQLGNDKIQASDGAFTTTVIKTTFGGVVYSG
                     TVVSSDYSELKWELTEETNVGFTAKFVKNKLSADIDYYNRDTKNAAIPVSIPSVGGSV
                     LKPVGTIRNSGIEIALNWSNEINGKLNYSVGANLATLKNQAIDLYGQEYIDGGTAEFR
                     QRTYVGEPLMAFYGWEVAGVYQNATEISNDPIAVDNNLVPGDFKYVDQNKDGLLDGDD
                     RVILGSYFPNLIYGGDISIRYLNIDFSASFYGQAGNKILNRKRGELIWTSDGNLDADL
                     AENRWYGEGTSDKYPSSSGLRRGWNQKMSDYFVEDGSFFRFQNIALGYTIKNSKWLGG
                     NFPETRISFTAERPLSLFKYNGFTPEVANGWDSQTYPVAAIYTVGLNIKF"
     gene            2567..4102
                     /locus_tag="PKM34_02670"
     CDS             2567..4102
                     /locus_tag="PKM34_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNQ82516.1"
                     /translation="MKPYKFIIVISIALTVLLSNTGCNKYLDEPPENTTFTEETDYTI
                     SSNMVLPLIGAYERMNLVDIGWENFPLVAVRGDDVNAGGLGDQQDFAETDKYNYNKDY
                     WMYNSLWQNYYLSMFIANSAIEQVELYKPHASASLAEQYQAEARVLRSWWLFQLSRTF
                     GALPVPESSDPSELFVVPLSTKDEVMQMISAWMDEAIPLLPAVRPNERTDIPGGVTKF
                     TALAMKALANLELKNYQAVAEATGEIINSGKFELEADYYELFKIKGKLNSENLLEMQY
                     SDLGQAAGDNYYYLFAFFGPQGWTPAVEGAGDGWGFWEPSMKYIKFMLDRNEVTRLKT
                     TVLFTPRGIAEIQADPNYVNLPSWISNVTPSGDVINDYARAMFCSGKHYLPSIQLTPG
                     RTSYGTNKNFQCIRYAEILLMYAEALANGASGSAGTADAAVNAVRERAGLSPLAGVNL
                     QQVLDEKYAELAMEWGTRFFDMVRYNRVDALSYEGRTFSADKAFLPYPQNQVDQIPVL
                     KGN"
     gene            4132..5202
                     /locus_tag="PKM34_02675"
     CDS             4132..5202
                     /locus_tag="PKM34_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ82517.1"
                     /translation="MKIIKSLFLLAMLTLVVASCEKELDPIIPVDPKPDQTAPTVAIS
                     YPIEGKAFVSPDEPATITFKVLAVDDIELQSVTLKLNGAEIVTFDSFLDYRRADIKYN
                     YSGMTAGTYTLSVDVVDLTGKTASTSVNFNKVTAPVYEPLAGEVLYFPLDGFNLDLVT
                     GAELAVNGAPGYAAGKVNDCYAGATDAYLTYPADAITSGTEFSVAFWYKINADPLRAG
                     ILAISPEGDSRNSGFRLFRENSGTNQNIGLNIGIGSTEVWMNPFVAVPADQDWMHIAI
                     VISTSKATIYVNGEIAPTNSEVDLESKIDWTGCSSMTIASGMPNFVYWEHFSDLSLYD
                     EMHFFTKALTPAEVLDLYTVKK"
     gene            5387..7369
                     /locus_tag="PKM34_02680"
     CDS             5387..7369
                     /locus_tag="PKM34_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNQ82518.1"
                     /translation="MKQTTIHEFIVMLLIMTIAVNLQSCNKEPEPVGKLEILSCFAGE
                     TQLQLAAEVPNIALNTEIILNFSVPLDTNLAKQGISIKDLNNATFGAQYKFSGDMKTL
                     RLTHQQEFGKLKSYTVFIAGTVKGTEGESFTGAEYRFKTSDGKFNLIAATIDGQDFTT
                     NTKFYNIKRKNLRFSLEFSEPVDTAGISAKFSLSDDIKEIFEYGDNFRKVDLTLQGDL
                     EGYRKYTLVVSSGLESAYGNQFEGFSNSFFTEADSTYKFPVVTDDELLTIVQQQTFKF
                     FYDYAHPVSGMTRERLGSGDVVTSGGSGFGVMALIVGMERGFITRAEGMARLEKIVGF
                     LETCDRFHGAWSHWINGSNGYAIPFSTKDNGGDLVETAYMAQGLLTMRQYLDPLDPAE
                     SDLATRITELFNTIEWSWYTRGGQNVLYWHWSPTYNWDMNMKIQGYNETLITYILAAS
                     SPTYTIPAMAYKSGYARNGGIINGKTFYGYTLPVGYDYGGPLFFAHYSFLGLDPRNLS
                     DTYANYWTQNVNHSLINWAYCADNPKSFPNYSAHCWGLTAGDIPSGYGVSEPTNDRGV
                     ITPTAAVSSLPFSPEQSMDAIRFFYYILGDKLWGDYGFYDAFDVHVGWWAGSYIAIDQ
                     GPQVVMIENYRTGLLWDLFMSAPEVQAGLDKLGFSY"
     gene            7486..>8198
                     /locus_tag="PKM34_02685"
     CDS             7486..>8198
                     /locus_tag="PKM34_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ82519.1"
                     /translation="MPRKKSKVAKIFVLDTSVILYNHNAIYSFEDNDIAVPITVLEEL
                     DNFKKGNDTKNFEAREFIRIMDRLSANHSLTNWIEIDQQGAGCIKVIMNENNSPIDAR
                     AVFDGNKPDHRILNAAMVMQDEHPQRPVILVTKDINLRLKAKSLDIQAEDYETGKIQD
                     VESLYPGKSTLEGVDKSVIDKLYSDGLVDPEDVGLTDPIPNHYFVLKNGNSSALAYFN
                     PLLGKIERVEKTQAYKITPR"
BASE COUNT         2200 a         2164 c         1946 g         1888 t
ORIGIN      
        1 cggcagtatg cccactatcc gtatccgcgg tatcggctca ttccccggac gggaaaatga
       61 agcgccgctg tttgtcgtcg acggcatgtt cttcgataat atcgactttc tgaatccttc
      121 cgacatcgcc tccatttctg tcatgaaaga cgcctcggca gccgctatat atggtgtcag
      181 ggccgccaac ggcgttgtac tcatcgaaac aaaatcagga caatacaacc agaaggctga
      241 aatcacctac aatggttatg gcggttacca gatcgcccag aatgtggtga agatggccaa
      301 ctcccagcag ttttccataa tggccaggga atcgggctca cccgccgatg aaacgttcat
      361 ccttaacgcc atgcaacgct acggcagaag ccgcgtagat cccagtatac cggaacccaa
      421 taccaactgg tacgccgaaa tcctccgccc ggccgccatc actaaccata gcctggattt
      481 ctcaggcggt acggagaaag cacgctattc cctcggcgcc aactacttct accaggatgg
      541 gatcatgaat atggacaata actacgaacg ccttaacctc cggaccaaac tcgattttaa
      601 ggccacaaac tggctgactg tcggcggtaa tatggtgtat agcaatgcat tgaaaaacaa
      661 tgagcaaaat tcggcatgga acaatgccta ctttgccgtc ccgatcatgc ccgtttacga
      721 taccttgaac gacctggctt atccggataa ttactccagt gcgcgtataa tgggttacag
      781 atcgggacag aatcccttcc cgtatatgga atacaatttt gaccagatga aaatcaaaaa
      841 gttgttatcc aatttctatg tcgaaatcaa ccttctgccg aaagttctta cttttaaaac
      901 atcatacaac tacagctata ccgggatcga tcaacgtatc atcaatttcc cgtattatat
      961 tgatgatggt ttcaataacc tcgacccgaa aattacccgc acctggacca attataacaa
     1021 ccatatctgg gataatatcc tgacattcac caagagcttc cagaatcata acctgacact
     1081 gatggccgga acttctttca aagacgaggg ttacacaatg cttagcgcct gggtcaacga
     1141 tatcccgacc gataaaccgc aatcctggta tatcaataaa accctggata tcgaatccat
     1201 tggcgccacc gacggtggcc tccgccaata cggaatgtct tacttcggac gtctggccta
     1261 caacttcaac aaccgctacc tgctctacgc aacgatgcgt gccgacggca gcaacaaata
     1321 ccagcaaacc tggggctatt tccccacggt cggtattggc tgggtcgtca ccgaagaaaa
     1381 gttcatgccg aaaaacgata ttcttactta tctgaagatc agggccagct ggggacaact
     1441 gggcaacgac aagatccagg ccagcgatgg cgcgtttacc actaccgtta taaaaaccac
     1501 cttcggtggc gtcgtctatt ccggtactgt ggtgtcaagc gattattctg agctgaaatg
     1561 ggaacttacc gaggaaacta atgtcggttt caccgcaaaa tttgtgaaga acaaattatc
     1621 ggctgatatt gattactata accgcgatac gaaaaatgcc gccatccccg tctctatccc
     1681 gtcggtcggt ggtagcgtgc tgaaaccggt cggtacgatc cgcaactccg gtatcgaaat
     1741 cgcactgaac tggagcaatg agatcaacgg caaactgaac tacagcgtcg gggcaaacct
     1801 ggctaccctg aaaaaccagg ccatcgacct ttacggacag gagtatatcg acggcggtac
     1861 cgctgaattc cgtcagcgca cctacgtcgg cgaaccgctt atggctttct acggatggga
     1921 agtggccggc gtttaccaga atgcgactga aatcagcaac gatccgattg cggtcgacaa
     1981 taatctcgtc cccggtgact tcaaatatgt cgaccagaat aaagatggtt tgctggatgg
     2041 cgacgaccgc gtgatcctcg gttcctactt cccgaacctc atctatggcg gcgatatcag
     2101 tatccgttac cttaacatcg acttcagcgc cagtttctac ggtcaggccg gaaacaaaat
     2161 cctcaaccgg aaaaggggag agctgatctg gacctccgac ggcaacctcg acgccgacct
     2221 ggccgaaaac cgctggtacg gagaaggcac ctccgataag tacccgtcat cgtcaggcct
     2281 ccgcaggggc tggaaccaga aaatgagcga ctatttcgtg gaagacggct ctttcttccg
     2341 gttccagaat atcgccctgg gctataccat caaaaacagc aaatggctgg gaggcaattt
     2401 cccggaaacc cggatctcat ttaccgccga acgcccgctg tcgctcttca aatacaacgg
     2461 cttcacaccg gaagtcgcca acggatggga ctctcagacc tatcccgtgg ccgcgatcta
     2521 tacggtcgga ctcaatatca agttctaacg ctaaatactt attgatatga aaccatataa
     2581 attcattata gttatttcaa tagcgctgac cgtcctgttg tcaaacacag gatgcaacaa
     2641 gtacctcgat gaaccgccag aaaatacgac gttcaccgaa gaaaccgact acaccatcag
     2701 cagcaacatg gtccttcctt taatcggtgc ctatgagcgg atgaacctgg tcgatatcgg
     2761 ctgggagaat tttcccctgg tcgcagtgcg cggcgatgat gttaatgccg gcggcctggg
     2821 cgaccagcag gattttgccg aaaccgacaa atacaactac aacaaggatt actggatgta
     2881 taactccctg tggcagaatt actacttgag catgttcatc gccaattcgg cgatcgaaca
     2941 ggtagagctt tacaagccgc atgccagcgc cagcctggct gaacaatacc aggccgaagc
     3001 cagggtgctg cggtcatggt ggctgttcca gctcagccgt acatttggcg cactgccggt
     3061 gcccgaaagc tccgacccgt cggaactctt cgtggttccc ctatcaacaa aggatgaggt
     3121 catgcaaatg atctctgcct ggatggatga agccataccg ctgctgcctg ccgtcaggcc
     3181 caacgaacgt accgatatcc cgggcggcgt cacaaaattc acggccctcg ccatgaaagc
     3241 cctggccaac ctggagctga agaattacca ggctgtggcc gaagccaccg gcgaaatcat
     3301 caattcggga aaatttgaac tcgaagccga ttactatgaa ttgttcaaga tcaaaggaaa
     3361 gctgaacagc gagaacctgc tcgaaatgca gtattccgac ctgggccagg cagccggcga
     3421 taactattat tacctgtttg cattctttgg accgcaggga tggacaccgg cggtggaagg
     3481 cgccggcgac ggatggggct tctgggaacc gagcatgaaa tatataaagt ttatgctcga
     3541 ccggaatgaa gtaacccgcc tgaagacgac ggttctgttc acccccaggg gaattgccga
     3601 gatacaggcg gatccgaact atgtgaacct gccttcctgg atttcaaatg tgaccccttc
     3661 gggtgatgtt atcaacgact acgcccgtgc aatgttctgc agcggcaagc attacctgcc
     3721 ttccatccaa cttacccccg gaagaacaag ctatggtact aacaagaact tccagtgtat
     3781 ccgctatgcc gagatcctgc tgatgtatgc cgaggcattg gccaatgggg catccggcag
     3841 cgccggaaca gccgatgcag cagtgaatgc cgtcagggaa agagcaggtt tgagcccgct
     3901 ggccggagtg aacctccagc aggtgctcga cgaaaaatat gccgagctgg ctatggaatg
     3961 gggaacccgg ttcttcgata tggtccggta caatagagtt gatgcactga gctacgaggg
     4021 aagaacattt tcagctgata aagcatttct gccttatccg caaaaccagg tcgaccagat
     4081 accggtatta aaaggtaatt aacctatggt ttaactttaa aaaaattcaa gatgaaaatt
     4141 ataaaatcct tgtttttact ggcgatgtta accctggtcg tggcctcctg tgaaaaggaa
     4201 ctcgacccga tcatcccggt cgatccgaaa ccggatcaaa ccgcgccaac ggttgccatc
     4261 agctacccga tagaaggtaa agccttcgta tcaccggatg aacccgctac cattacattt
     4321 aaggttctgg cagtggatga tatagaactc caatcggtta cattgaagct taacggcgcg
     4381 gagatcgtaa catttgactc tttcctggat tacagaagag ccgatatcaa atacaattat
     4441 agcggtatga ctgcaggtac ttataccctg tcggttgatg tggtcgacct taccgggaaa
     4501 acggcgtcga catcggttaa ttttaacaaa gtgaccgcac cggtttatga acccctcgcc
     4561 ggcgaagtgc tctatttccc gctcgatggc ttcaacctcg acctggttac cggcgctgaa
     4621 ctggcggtta acggcgctcc gggatatgca gccggaaaag tgaacgactg ttatgccggg
     4681 gctaccgacg catacctgac ctacccggct gacgcgatta cctcgggaac agaattcagc
     4741 gtggctttct ggtataagat caatgccgat ccgcttcgtg ccgggattct ggccatcagc
     4801 cccgaaggcg acagccggaa cagcggcttc aggttgttcc gcgaaaatag cggcaccaac
     4861 cagaatatcg gactgaatat cggtatcggt tccaccgagg tctggatgaa cccgtttgtc
     4921 gcggttcctg ccgaccagga ctggatgcat atcgccatcg tgatctcgac ctcgaaagcc
     4981 actatctacg tcaacggcga gatcgccccg acgaactccg aagtggacct ggaatccaaa
     5041 atcgactgga ccggatgcag cagtatgacc atcgcttcag ggatgccgaa cttcgtctac
     5101 tgggagcatt tctccgacct gagcctctat gatgagatgc atttcttcac aaaggccctg
     5161 accccggctg aggtactgga tctctatacc gttaaaaaat agagtcggta ttcaattctg
     5221 tcgctataag tcattttaag gttctttaac cacaaaggcg tacaaagttt gatatttggt
     5281 aatcaattac tttgtgcgcc tttgtgaatc cctttgtgta actttgcggt taagtaattt
     5341 ttaatgaccc ggatgaatta attgcggatg taataatttt ataaagatga aacaaaccac
     5401 aatacatgaa tttattgtca tgttattgat catgacaatc gcagttaatc ttcaatcctg
     5461 caacaaagaa cccgagccgg tcgggaaact cgagatcctc tcctgttttg ccggtgaaac
     5521 ccagcttcag ctggctgccg aggtccccaa tattgccctg aataccgaaa tcatcctgaa
     5581 cttttcggta ccccttgata ccaacctggc taaacagggc atctccatca aagatttgaa
     5641 taacgccact ttcggggcgc aatataagtt ttccggggac atgaaaacat tacggctcac
     5701 ccatcagcag gaattcggaa aactgaaaag ttacacggtt ttcatcgccg gcacagtgaa
     5761 aggaacagaa ggcgaatcgt ttacaggagc ggaatacagg tttaaaacat cagacgggaa
     5821 gtttaatttg atagctgcaa caattgatgg tcaggatttt acgactaata ccaagtttta
     5881 caatataaaa agaaaaaacc tccggttttc gctggaattt tcagaaccgg ttgataccgc
     5941 cggaatttca gcgaagttct ccctttcaga cgacattaaa gaaatttttg aatacgggga
     6001 caatttccgg aaggtcgacc tgacacttca gggtgatctg gaaggatacc gcaaatatac
     6061 cctggtagtc tcatcaggcc tcgaatcagc ctacggaaac cagttcgagg ggttcagcaa
     6121 ttctttcttt accgaagccg actccaccta caagttcccg gtagtaacgg atgacgagtt
     6181 gctgaccatc gtccagcagc aaacgttcaa gttcttttat gattatgccc atccggtgag
     6241 cggaatgacc agggaaagac tcggctcggg agatgttgtt acttctggcg gctccgggtt
     6301 cggggtcatg gcgcttatcg tcggcatgga gagaggattt atcacccggg ccgaaggtat
     6361 ggcccggctg gagaagatcg tcggattcct ggaaacctgc gaccggttcc acggagcctg
     6421 gtcgcactgg atcaacggca gcaacggcta tgctatcccg ttcagcacca aagacaatgg
     6481 cggcgacctg gtcgaaaccg catacatggc ccaggggctc cttactatgc gacagtacct
     6541 cgatccgctg gatccggcgg aaagtgatct ggcaacaaga atcactgaac ttttcaacac
     6601 gatcgagtgg agctggtata cccgaggcgg gcaaaatgtt ctttactggc attggtcgcc
     6661 aacctataac tgggatatga acatgaaaat ccagggctac aacgagacac tgatcactta
     6721 catcctcgcc gcttcctcgc cgacctacac catccccgct atggcctata agtcaggcta
     6781 cgcccgcaac ggagggatca tcaacggcaa aacgttctac ggctataccc tccccgtagg
     6841 ttatgactac ggcgggccgc ttttctttgc ccactattcg ttcctcgggc tcgatccaag
     6901 aaatctttcg gatacctatg ccaattactg gacgcagaat gtcaaccact ccctgatcaa
     6961 ctgggcctat tgtgccgaca atccgaaaag ctttcccaat tacagcgcac attgctgggg
     7021 attgaccgcc ggcgatatcc cttccggata cggcgtgagc gaacccacca acgaccgggg
     7081 cgtgatcacc ccgaccgcgg cggtctcctc tctccctttt tctccggaac aatcgatgga
     7141 cgctatccgg ttcttctatt atatcctcgg agataaactc tggggcgatt atggattcta
     7201 tgatgccttc gatgtccatg tgggctggtg ggccggctcc tatatcgcga tcgaccaggg
     7261 accccaggtc gtcatgatcg aaaattaccg taccggactt ctctgggatc tctttatgag
     7321 cgcccccgaa gtccaggccg gcctcgataa actgggcttc agctattagt gttgataaat
     7381 attcaaaatg acaaatttcc ttattgacta tttttacatc ccattatcat tatgaattaa
     7441 gaattaagaa tttaaaattt aaaatttaga attaactctc catagatgcc cagaaagaaa
     7501 agtaaagtcg ctaaaatatt cgtcctcgat acgtcggtca ttctttataa ccataatgcc
     7561 atttatagtt ttgaagataa tgatattgcc gttccaatca ctgtgctgga agagctcgat
     7621 aatttcaaga aagggaacga taccaaaaac tttgaggccc gtgagttcat ccgtattatg
     7681 gaccgcctct ctgccaatca ttcgctgacc aactggattg agatcgatca gcagggagcc
     7741 ggttgtatca aggtgatcat gaatgagaac aacagcccga tcgacgcccg ggcggttttc
     7801 gacggcaata agcccgacca ccggatcctg aatgccgcca tggtcatgca ggatgaacat
     7861 ccgcaacgtc cggtcatcct cgtgacaaaa gatatcaacc ttcgccttaa ggccaaatca
     7921 cttgatatcc aggcagagga ttatgaaact ggcaagatcc aggacgtcga atcgctctat
     7981 ccgggcaaat cgaccctgga aggcgtcgac aaaagcgtga tcgacaaact gtactccgat
     8041 ggtctggttg atcccgaaga tgtggggctt accgatccca tcccgaacca ctattttgta
     8101 ctgaaaaacg gcaactcctc ggccctggcc tatttcaacc cgctccttgg aaaaatagag
     8161 agggtggaaa aaacgcaggc ctacaaaatc accccgcg
//
LOCUS       DAOQXS010000054         8173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9261_length_8173_cov_3.343927, whole genome shotgun sequence.
ACCESSION   DAOQXS010000054 DAOQXS010000000
VERSION     DAOQXS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9261_length_8173_cov_3.343927"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKM34_02690"
     CDS             <1..474
                     /locus_tag="PKM34_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ82520.1"
                     /translation="DFRSATMLGNLLIFINAASWSFYLVLIKPLMERYDNFTVMKWVF
                     LFGFIIIFPFTFNSFASSSFSTIPVNIWLSISFVVFGATIIAYFLNNYSLKSVSPSVN
                     GIYIYLQPLIASVVSILFGKDQISVIKILAAILIMTGVYLVTRRSRKHSPGFPER"
     gene            525..1205
                     /locus_tag="PKM34_02695"
     CDS             525..1205
                     /locus_tag="PKM34_02695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNQ82521.1"
                     /translation="MKKYYFSAVLLFALTLSASAQDTVKVALKTSADSLSFSFGYLIG
                     NNLFVQNVENINQKIFMKGFNYGYSDQKTTLSVEEANRIIQHYFQNMISDESKQNLEK
                     SNAFLEENKLKEGVTTLPSGLQYTIITAGTGESPKPEDKVKVHYTGHLIDGTVFDSSV
                     ERGEPIVFGVNQVIPGWTEALQLMQPGAQWILYIPPALAYGENGAGGVIGPNQALIFE
                     VELIEVVK"
     gene            complement(1198..2166)
                     /locus_tag="PKM34_02700"
     CDS             complement(1198..2166)
                     /locus_tag="PKM34_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ82522.1"
                     /translation="MKEIRDHISRLDFEQLVKPIEELKNCRICPRNCNADRFSDKRGY
                     CKAGTDFTISSIFPHRGEEPVISGDRGICNIFFTNCNLQCIYCQNHQISCNSLDYGGN
                     RLSLPEVLERIATILATGINHVGFVSPGHFIPHVKVIVNALREMGSDPVFVYNTNGYD
                     TVESIRGLEGYIDIYLPDYKYSDENLGRKYSDVKNYPTVALEALKEMVRQKGTGLPVD
                     NRGYATNGVIVRHLVLPGHPVNSINVLRTIAYEVSNQLHISLMSQFYPTYLVQNHESL
                     GRTISEKEYQRVTEALHKLGFSKGWVQDFSSHESYRPDFEEETPFI"
     gene            2401..5043
                     /locus_tag="PKM34_02705"
     CDS             2401..5043
                     /locus_tag="PKM34_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ82523.1"
                     /translation="MTSKFPVTYPLLTFRLDNELISLADAKAYCTDSSIIFDYKGLIT
                     GEIIADNEFQEVWQRRVILRNPTDDTIDIENLVPFGEIPDHVFLTAQGPWALARAWLF
                     MPAQEPVSVILPDNAWEMGYGALELSDTLGICAIARRVGTEKAESRRYRTIIYPGGSV
                     EYVLWVDEFTGKWQDGLKKMFREHYLFDLETFDETLYQRKDLHWIRRSYLIGLQFAWN
                     REFYDWKEQRYTADSFLEKGQELFGGYDVIGLWPTWPRLGVDRRNQWDMFADLPGGLE
                     KIREFSVNWKQKGTKFFICYNPWDESTRTENPYQGMARLIEATDADGVVLDCHGASSH
                     ALQHAADSVKAGVIMYSEGMAVVKDMPGIVAGRVHDAIVMSPLLNLNKMIRPDFGIFR
                     VCQLHDGRIRREVSIALFNGYGTELNTFAPGQAANMENDLVYLGKTTQILRENTSCFT
                     SVAWTPLINTTYDGIWVNEWPEEKGKTLFTVLSLRPEGFSGPLFKYETGQGKHAISLW
                     HHEELDWIEKEGEIYIPAKLEAFNSFDIGTRAESSVDCIAILPELLNVKLESGILNII
                     AQAGDEIRIWAGNPSYQNKACRIFPPGIYRIDVTDIFDGFEGKIVVQLFDDHELADER
                     IVVLEPGSPRLVRETIIPGTDKNYTGDMIRIPGGTFYFRAENTDQFIPYPDTRDAVTR
                     EVTSFYIDKYPVTNQKFLEFLKDSGYRPSDTVNFLKHWQDGTIPKGLEDHPVVYVSLE
                     DARVYAEWAGKRLPTEVEWQYAAQDTDGRIWPWGSEFDSTRCNNGSGFTTAVDAFPLG
                     ASPFGVMDMTGNVWQLTGDVYDNGTYYFVIMKGGSFYKPTSSWWYVKGGPQPNTWHQQ
                     LLLVSPGFDRCATVGFRCVKDSGE"
     gene            complement(5044..5784)
                     /locus_tag="PKM34_02710"
     CDS             complement(5044..5784)
                     /locus_tag="PKM34_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82524.1"
                     /translation="MKSKAMFILAGFICLFFETGAQVDGIKTTVNPSSKFKQTDSPTA
                     RQDLAMLQDHNTTGGGAGAASFGGQSGGIYLEPGWAPGSVTLRNNKVIDNIMLRYDIY
                     HQQLQFIQDEDTLAFARPEEAEFFLIGGKKFIYAEYLNGAVIGKSYFEVLSEGRCELL
                     IRRVIRYHMNEESKSGQQDEAFIRECEFYLVKPGERAKPVATGRKSILCAFSDKEAEV
                     KNFMEEKNLKMKDCNQMKLVVDFYNSLQ"
     gene            6047..6553
                     /locus_tag="PKM34_02715"
     CDS             6047..6553
                     /locus_tag="PKM34_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNQ82525.1"
                     /translation="MEWLLIVIGILLVLAGIAGAVLPGLPGPPLSFIALVLLQFRDEP
                     AFGEDFMILMGLIMIAVTVLDYVIPVYGAKKFGGTKKGVWGSTIGLVAGLFLLPALGI
                     VIGPFGLIGIIIFPFLGAYIGEKMSGRDSDTALKAAFGTFIGFLAGTVMKLVYSIVAA
                     VYFFIGIF"
     gene            6874..7548
                     /locus_tag="PKM34_02720"
     CDS             6874..7548
                     /locus_tag="PKM34_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82526.1"
                     /translation="MPLNKKLERKFRKTYGIDLLPVGMADTLIADIVEWNGAIGRTVD
                     LEHISLLEFLKIDEPTRKRLKEAIAGLPLHKAAMQQITIDSTFNISGGVDIPNWAVGI
                     GAEIGLKNFISFRIEDVQCRVLGRELRAELLRILLRAKETDLKYYRKKLSRRFIIEEL
                     FYAGKVSFEIKAGSTGQLDVTLTKAKIADPHITYSTEGNAIVTFPGDLTVPFAADIEP
                     VKDLMD"
     gene            7902..8123
                     /locus_tag="PKM34_02725"
     CDS             7902..8123
                     /locus_tag="PKM34_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82527.1"
                     /translation="MKMRRIIICIIAFAGVLYFSASLFGQDNCTVNNKPKNKKGINSY
                     TFEFDKEGKLISNELPEYLVGGDSIIFKL"
BASE COUNT         2211 a         1829 c         1919 g         2214 t
ORIGIN      
        1 gatttcaggt cggcaaccat gctggggaac ctgctcatct tcatcaatgc agcctcatgg
       61 tcgttctacc ttgtcctcat aaaaccgctc atggagagat acgacaactt taccgtcatg
      121 aaatgggttt tcctgttcgg attcatcatc atcttcccat tcaccttcaa ttcatttgcc
      181 tcgtcgtcat tctccactat accggtgaat atctggttat ccatctcttt tgtcgtattt
      241 ggcgcaacca tcattgctta ttttctgaat aattacagcc tgaaatcagt aagccccagc
      301 gttaatggaa tttacattta ccttcagccg ctgatcgcat cggtggtatc catccttttt
      361 ggcaaagatc agatctcggt catcaagatt ttagccgcca ttctgatcat gacgggcgtt
      421 tatctggtaa cgcgccgctc ccgaaaacac tccccgggct ttccggaaag ataatatttt
      481 ttgcctcgtc gggaaaaagt ctaattttgc caaaattttt aaaaatgaaa aaatactatt
      541 tctcagccgt attattattt gcattaacct taagcgcttc tgcccaggat accgtaaaag
      601 tggccctgaa gacatccgcc gactctcttt cattcagctt cggctacctg atcgggaaca
      661 acttatttgt ccagaatgtc gaaaatatca atcagaagat tttcatgaaa ggttttaatt
      721 atggatattc ggaccagaaa accaccttat cggtggaaga agcaaaccgg atcatccagc
      781 attattttca gaatatgatc tcagatgaat caaaacagaa cctcgaaaag agcaatgctt
      841 ttcttgagga aaacaaattg aaagagggcg tcaccaccct tccgagcggt ctccaatata
      901 cgattatcac agcgggtacg ggcgaatcgc caaaaccgga agataaagta aaggtgcact
      961 ataccgggca tcttatcgac gggactgttt tcgacagttc ggtagagcgc ggcgaaccga
     1021 tcgtattcgg tgtaaaccag gttattcccg gatggaccga agcgttgcaa cttatgcagc
     1081 ccggagcaca atggattctt tatattccgc cagccctggc ttacggtgaa aacggcgccg
     1141 gcggcgtcat aggcccgaac caggccctca tcttcgaagt ggaactgata gaggtggtta
     1201 aataaagggt gtttcttcct caaaatcagg tctatagctt tcatggcttg agaagtcctg
     1261 cacccagcct ttactgaaac caagcttatg gagggcttcg gttacacgtt gatactcctt
     1321 ttccgaaatg gtcctgccca atgattcatg attttgtaca aggtatgtgg gataaaattg
     1381 cgacatcagg gaaatatgca actggtttga cacctcgtat gcaatggttc tcaaaacatt
     1441 gatgctgttc accgggtgtc cgggcagcac caggtgcctg acaatcaccc cgttcgtagc
     1501 ataacccctg ttatccaccg gcagaccggt ccctttttgc cttaccattt cttttaacgc
     1561 ttccagggca acagtagggt agttctttac atcagaatat tttctcccca ggttttcatc
     1621 cgaatattta taatcaggaa gataaatatc gatgtaacct tccaggccgc gaatgctttc
     1681 aacggtatca taaccatttg tattataaac aaatacagga tctgacccca tctcacgcaa
     1741 cgcattgaca attactttga catgcgggat aaaatggcct ggggatacaa acccgacatg
     1801 gttaatccct gttgctagta tcgttgcaat gcgttcaaga acctccggca acgacagcct
     1861 gtttccaccg taatccagtg aattgcagct gatctgatga ttctggcaat agatacactg
     1921 aagattgcaa ttcgtaaaaa aaatattgca aataccccgg tcgcccgaaa tgaccggttc
     1981 ttcacctcta tgcgggaaaa tggaggaaat ggtgaaatca gtaccggctt tgcaatagcc
     2041 ccttttatcc gaaaaccggt cggcgttgca attccgcggg caaatcctgc aattcttcag
     2101 ttcctcaata ggcttaacca attgttcaaa atcgagcctg ctgatatgat cccggatttc
     2161 cttcatcttg tgtccctgag tgcactatgt gtccgaatag taaatttgat aacagcaaag
     2221 ataaacttgg aaacatcttc ttaacccatt aaatttgtag aaaccaaaaa ctgagttatg
     2281 caccggctta atcttttact cgcatttttc tttatctgca tccttcagac tattcattct
     2341 caggatattg cattatcagt aaaaaaaggt gtaatcctaa acagctatac tttaaatggc
     2401 atgacgagta aatttccggt tacttatcct cttttgacct tcaggttaga taacgaatta
     2461 atcagtcttg ctgatgcaaa agcctattgc actgattctt ccattatttt cgattacaag
     2521 ggattgatta ccggtgagat catcgcggat aatgaatttc aagaggtctg gcagcgccgg
     2581 gtcattctac gcaatccaac cgatgatacg atcgacattg aaaacctggt ccctttcggc
     2641 gaaattcctg atcatgtatt tctgactgca cagggtccgt gggctctggc aagggcatgg
     2701 cttttcatgc cggcacagga acccgtcagc gttatcctgc ccgataatgc ctgggaaatg
     2761 ggttatggag cgctggagct cagcgatacg cttggcattt gcgccattgc acgaagggtc
     2821 ggcaccgaaa aagccgaatc caggcgttac cggactatca tttatccggg tggatcggta
     2881 gaatatgttt tatgggtaga tgagttcacc ggtaaatggc aagacggatt aaaaaaaatg
     2941 ttccgggagc attatctgtt cgatcttgaa acattcgacg aaacgctgta ccaaagaaaa
     3001 gacctgcatt ggatccggcg atcgtacctg atcgggctgc aatttgcctg gaaccgtgaa
     3061 ttttacgatt ggaaagagca aagatacaca gcggattcat ttctcgaaaa aggccaggaa
     3121 ttatttggcg gctatgatgt gatcggctta tggcctacct ggccgcggct gggagttgac
     3181 cggcgcaacc agtgggacat gtttgccgat ttgccgggag gattggaaaa gatcagggag
     3241 ttctcggtaa attggaagca gaaagggacg aagtttttca tttgctataa tccctgggat
     3301 gagagcacac gaacggagaa tccataccaa ggcatggcgc ggctgatcga agcgacagat
     3361 gccgacggag tggtgctgga ttgccacggc gcttcgagcc atgcactcca gcatgcggcc
     3421 gacagcgtga aggccggcgt gatcatgtac agcgaaggga tggctgtggt gaaagatatg
     3481 ccggggatcg tcgccggccg ggtgcatgac gccattgtca tgtcgccact gctcaacctg
     3541 aacaagatga tccgccccga cttcggcatt ttccgggtat gccagctgca cgacggccgg
     3601 atacgccgtg aagtttccat tgcccttttc aacggttatg gcaccgagtt gaacaccttc
     3661 gccccggggc aagcggcaaa tatggagaac gacctggtct acctgggaaa gaccacgcaa
     3721 atcctcaggg aaaatacatc ctgctttact tctgttgcct ggacacccct gatcaacacc
     3781 acgtacgacg gtatctgggt aaacgaatgg ccggaagaaa aaggcaaaac cctgtttacc
     3841 gtgctgagcc tgaggccgga aggattctcc ggcccgttat tcaaatatga aacgggacag
     3901 ggtaaacatg ccatcagcct gtggcatcat gaggaactgg actggattga gaaagaagga
     3961 gaaatttata ttcctgcaaa gctggaagcg ttcaattcgt ttgatatcgg aacgcgggca
     4021 gaaagcagcg tagactgcat tgctattttg ccggaattac tgaatgtcaa actggaatcg
     4081 ggtattctga atatcattgc tcaggctggc gatgaaatcc gcatctgggc tggtaatcca
     4141 tcgtatcaga ataaagcatg cagaattttt ccaccgggga tctaccggat cgatgtgacc
     4201 gatatttttg acggctttga aggaaagatt gttgtccagt tattcgatga ccatgaactg
     4261 gcagatgaaa ggattgttgt gctggaaccg gggagtcccc ggcttgtgcg ggaaaccata
     4321 atacccggaa ccgataagaa ttacaccgga gatatgatcc ggatcccggg tggaacgttt
     4381 tatttccgtg ctgaaaatac cgatcagttc atcccctatc ctgataccag ggatgcggta
     4441 accagggagg ttacatcgtt ttatatagat aaataccctg tgacaaacca aaagttcctg
     4501 gaatttctga aggattccgg ttaccggcct tccgataccg tcaatttcct gaaacactgg
     4561 caggatggaa cgattccaaa gggtttggag gatcaccctg ttgtgtatgt cagcctggag
     4621 gatgcccgtg tctatgcgga atgggcggga aagagactcc cgacagaagt cgagtggcaa
     4681 tatgccgcgc aggatacgga tgggcggatc tggccgtggg gcagcgaatt tgattccacc
     4741 cgctgcaata atggctccgg gttcacaacg gcagtcgatg cctttccttt gggcgccagt
     4801 cctttcgggg tgatggacat gacgggaaat gtgtggcagc tcaccggtga tgtttacgac
     4861 aacggcacct actattttgt gattatgaaa ggcggcagtt tttataaacc aacatcgtcg
     4921 tggtggtacg taaaaggcgg gccgcaaccc aatacctggc accagcagct cctgctcgtc
     4981 tcacccggat tcgaccgctg tgccacggtc gggttcaggt gcgtgaaaga ttccggagaa
     5041 tgattactgc aaactgttat aaaaatccac caccagtttc atctggttgc aatctttcat
     5101 tttcaaattc ttttcctcca tgaagttttt tacttctgct tctttatcac tgaaagcaca
     5161 aaggatactt ttccgtcccg ttgcaaccgg tttggcccgc tcgccgggtt tgaccaggta
     5221 aaactcacat tcccggataa aggcttcatc ctgctggccc gactttgatt cctcattcat
     5281 atgataacgg atcaccctcc ggatcaacaa ttcacaccgg ccttccgaga ggacttcaaa
     5341 atagcttttc ccgataactg ctccgttaag gtattccgca tagatgaatt tctttccgcc
     5401 gatcaggaag aactccgctt cttcaggcct tgcaaatgcc agggtatctt catcctgaat
     5461 gaattgtaac tgctgatgat aaatatcata tctcaacatg atattatcga taaccttatt
     5521 attacgaagc gtgactgaac ccggagccca accgggctcg aggtagatgc cgccggactg
     5581 gcctccgaat gatgcggcac cggctccccc gccggtagta ttgtgatcct gcaacatggc
     5641 cagatcctgc cgtgcagtgg gagaatccgt ctgtttaaat tttgatgacg ggttaacggt
     5701 ggttttaatc ccatcaacct gggcaccggt ttcaaaaaac aggcaaatga aacccgccag
     5761 aatgaacatc gctttacttt tcatggctca atagattaaa ttactgatta tcaatagata
     5821 ttatcagctc ttactctata aagataatca ttttcgagcc tatttcaaag catatatcga
     5881 aatattttat atatagacgt aaggatgcta ttgattatta cattttttaa ctttttttat
     5941 gaaatcattt ttacaatgac tcaatcaatc aggtacaatg gcttcatttc tgctttaagc
     6001 ttgttttcag ggaatgcaat attatttata cttttagagg acgattatgg aatggttgct
     6061 gattgtcatc ggaattttat tagtcctggc gggcattgcc ggggcggtat tgcccggact
     6121 tcccgggccg ccgctgagtt tcatcgcgct ggtattgctt cagttcaggg atgagcccgc
     6181 attcggagag gactttatga tccttatggg attgatcatg atcgctgtta cggtattgga
     6241 ttatgtgatc ccggtgtatg gcgccaaaaa gttcggcgga acgaaaaaag gggtatgggg
     6301 aagcacgatc gggctggtgg caggcctttt tcttttgccg gcactgggga tcgttatcgg
     6361 gccatttgga ctgatcggca taatcatttt ccctttcctg ggagcctata tcggggagaa
     6421 gatgagcggc agagacagcg atacggcttt gaaagcggct tttggcacat ttatcggttt
     6481 cctggccggg acagttatga agctggtata tagtatcgta gcggcggtgt atttctttat
     6541 cgggatattt tgattttgtg atattatgag cttgtgattt tgtgatatta tgagcttgtg
     6601 atattgtgag cttgtgattt tgtgaacttt aaaaaatagt tttgttaata aattcttttg
     6661 ggaaaagcag gtgagctatt gacaagggca gattggttcc ttatatttgt ttcgcctgaa
     6721 catcactaac ttactaaaag aagaaaaagg atcaatttag cagacgctta cgcgggcatg
     6781 ttgaattttt ctgataatcc ccccgaactc ctttactaca agtgctaagt gcaacagact
     6841 gatctctact tagtcataac aatagtaatc attatgccat taaacaagaa actcgagcgc
     6901 aaattccgga aaacatacgg gattgacctg ttgccggttg gtatggcgga tacgttgatc
     6961 gccgatatcg tggagtggaa cggggcgatt ggcaggacgg ttgatctgga gcacatcagc
     7021 cttctggagt ttttaaagat cgatgagccg acccgaaaac ggctgaagga agctattgcc
     7081 ggtttgccgc tgcacaaagc cgctatgcag cagatcacca tcgacagcac gttcaacatc
     7141 agcggcgggg tggatatacc caactgggcc gtggggatcg gcgcggagat cggattgaag
     7201 aacttcatca gtttcaggat cgaggatgtg caatgccggg tactgggaag ggagctgcgg
     7261 gcggagctgc tgcggatcct cctccgggct aaggagaccg acctgaaata ttaccggaag
     7321 aaactctcca ggcgctttat catcgaagag ctgttctatg ccggcaaggt gagctttgag
     7381 atcaaagccg gctcgacggg ccagctcgac gtcaccctga ccaaagcaaa gatcgccgat
     7441 ccgcacatca cttactccac cgaaggcaat gccatcgtca ccttccccgg cgacctgacc
     7501 gtgcctttcg ccgccgacat cgagccggtg aaggatttga tggattgata ttgtatgtca
     7561 tttaaatatt ggtgaagccg aaaaccagag aaagagtagg cgaaatatta aggaaaatag
     7621 atgcatgaaa tccaattcac atttaacgcc agtactgtcc ggttgtcggg tatttgaaaa
     7681 catgtaacta actataattc tacctataaa accatgtttt tgaattttgc atttttgata
     7741 aaacgcctat aaatgagcta tttaccaata acaataagta caatttcaac acactcaatg
     7801 atgttcaact gaatctattc aatttataac cggacagtac tggtaaaggt ttatagttaa
     7861 ataagaattg ttattcaaat attaatctaa tcaatattta aatgaaaatg agaagaatta
     7921 taatctgcat tattgcattt gctggagtat tatacttttc agcaagttta ttcggacagg
     7981 ataattgcac tgttaataat aagcctaaaa ataaaaaggg cataaattca tatacatttg
     8041 aatttgataa ggaaggaaaa ctaatttcaa atgaattacc ggaatatctg gtcgggggtg
     8101 actctattat tttcaaatta taatactccc caaaattcag accacaggtt aagttgaaaa
     8161 acgactaatt tta
//
LOCUS       DAOQXS010000055         8138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9310_length_8138_cov_3.711988, whole genome shotgun sequence.
ACCESSION   DAOQXS010000055 DAOQXS010000000
VERSION     DAOQXS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9310_length_8138_cov_3.711988"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="PKM34_02730"
     CDS             <1..1518
                     /locus_tag="PKM34_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82528.1"
                     /translation="QDENGNYTREIGAYEPNYMAFYLQDKFAFRDLIFNVGIRADRFD
                     ANQQMLKDPFLVYQAYTVKEVSSVGGDAVSHPSNMGSDYVVYVDNVNNPTRVVGYRNE
                     YDWFNASGAEIQDPNSLDVGSGISPYLVDPTLERPTIAAFKDYEPQWSVMPRIAFSFP
                     ISDEALFFAHYDVLTQRPTNSVFSDPSTYYFFNNVSGTINNPGLKPTQTIDYEVGFQQ
                     KLTNSSSVKLVTFYREMRDMLQIYRFNGAYPKDYTSFNNIDFGTVKGLTVQYDLRKTG
                     NVRLSAYYTLQFANATGSNQGTTAALVASGLPNLRSTFPIQTDRRHSFNIFLDYHFGG
                     DAEYNGPVSQRKKSGKPPIQWLSYFGFTVTLNGGSGTPYTQSRNVVGINQAGTNLVKG
                     TYYGARGPWQFRTDATIDKDFHFMLGNGENQRPAFVNVYLRINNILNQKNIMGVYPFT
                     GNPDDDGYLSAPESQKQISEATSEQAYRDLYAIAVNNPGNYSSPRTIRLGVIFNF"
     gene            1569..5870
                     /locus_tag="PKM34_02735"
     CDS             1569..5870
                     /locus_tag="PKM34_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82529.1"
                     /translation="MRIIYRLLSVVLIGLLLTGGAYSREFVGAKKNAGTIKGTAAGCS
                     AASGFRFLDINNIRARINTGGDMWWDLPGGIGAQYYIPKTGTATSMFSGSLWIGGLDI
                     NNQLKLAAIRYRQVGNDYWPGPLTTDGTAAVDQETCAKYDKHFLITRLEVDEFLGWWN
                     STNKAEEYPGYSIPKSILEWPAHGDVAKLNSYYLAPFRDVDGDGEYDPNNGDYPYYDI
                     TNELCPINFAGDPSYIPARTMEEDLTEYKVALTGSILVDQVIKGDQTLWWVFNDKGNT
                     HTETQGAPIGMEVRAQAFGFATNDEINNMSFYSYEVINRSTFELTNTYFCPWVDTDLG
                     YAWDDYVGCDVLRGLGYCYNGVAIDGSGEPESYGAQPPAIGVDFFQGPYIDPDGFDNP
                     RFTGDCSILGQGPNFEVDQMAINGVNFGNGIVDDERYGMRRFVYHNNTSSNPATTDPG
                     VASEYYNFLRGIWKDNSLMLYGGTAHNIDAAAVGPICEFMFPGDSDPCNWGTKFQPPN
                     GGYNQNGKYWTEEESGNEPGDRRFMQSAGPFTLKPGAVNYITVGIPWARAASGGPWAS
                     VELLRVVDDKCQALFDNCFKVIDGPDAPDLIIRELDRELIVFIDNPKGSNNYLEQYAE
                     FDPNIIQPNPANSELRSDSIYRFEGYQIFQLKDASVTLGDAKDEYGNFNPDMVRLVAQ
                     FDVKNGVGRIINHYFNKAIGFNIPVVEVEGGDEGIQHSFRITDDAFASGDKRLVNHKQ
                     YYYTVVAYAYNEYLPYDPEDVIGKMGQKKAYLPGRKNIVTYTAIPHKTINGLVMNSTY
                     GDGPAITRIEGQGNDGMILDLTPETIEEILSKQPVGTVIDGDTVDIGHPLYPIAYEAK
                     YVNGFGPLNIKIIDPLNVPTGKYYVRFDTLYTMRNFNVTGNTALIAGGDTASYITGSW
                     TLVDSLGREFKSDATLDKQNEQLFLDFGISINMKSFYTPGPKKVGQKPDQNGPITIYE
                     VLADNNGVLESSVTYQDSSYIWLGGVVDNDVPNDPRNWIRAGTYQDGNSSDWNMPSAS
                     FDPNQNFERLAGGTWAPFVLTAANDQDINGPAYSIACKERTFMEDVFSVDIVFTPDKS
                     KWSRSLVIEMCPDANLSQGKAARFQFRRAPSIDKDGNPASGGSGPSSDPNAANYVSDS
                     SMSWFPGYAINVETGERLNIVFGEDSWLVADNGRDMKYNPTSTIFDPVSGNARFGGKH
                     YVYIMDHVRRDLQGRIFDFPAYDGCKYIRNGYGLNDLPPFLYELAWMSTAMWVGMPLS
                     IDGKEWLNNEVKIRIRVAKPYKRFYSTTLDSASIANSQNGHMPMYQFETQSVATQLSD
                     PEKAKSDLDLIQVVPNPYYAYAGGEGYERNALDNRIKITNLPERCTISIYNVNGTLIR
                     QITKDEPKTSVDWDLKNFAGVPIAGGVYIIHVKADNGEKILKWFGGLRVPDLNVF"
     gene            5912..6979
                     /locus_tag="PKM34_02740"
     CDS             5912..6979
                     /locus_tag="PKM34_02740"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNQ82530.1"
                     /translation="MKNLYRYLAALTMITALFFATTGVQAGNKDRSGQAGAPELLINP
                     WTGSAGWGGVNTSNIRGVESMFANIAGLAFVNKLELGFSQTYWLKGSGIGISNFGLGV
                     RAGETGAIGISVFSMRFGDLDVTTTEQPDGTGATYSPSLLNFALSYSKTFSNSIYAGL
                     TIRIISESIPDASAQGICLDAGVMYVTGERDQAKFGIALRNLGPTMKFTGDGFSIKAF
                     FQGNDNSISVIQRSESFELPTQLRIGASYDFQIAALHRLTLAGNFTSNSFSKDQFTLG
                     LEYSLNEILMLRGAYTYEDGILEDVSDPSRTNAYKGLSLGASVEVPLSKKTDLKLGID
                     YAYQSTDNFKGTQAFGIRIKY"
     gene            7073..7549
                     /gene="greA"
                     /locus_tag="PKM34_02745"
     CDS             7073..7549
                     /gene="greA"
                     /locus_tag="PKM34_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNQ82531.1"
                     /translation="MAEPKYYTKEGLDKLRAELDHLTTVERPSISKQIAEARDKGDLS
                     ENAEYDAAKNAQGMLEMKIARLQEEISNARIIDESKMDLNKILILSTVKLSNLKNGAV
                     MSYTIVPESEANLKAGKISVSSPIAKGLLGKTVGDKVEIDVPAGKLNFEIIEISRS"
     gene            7560..7967
                     /locus_tag="PKM34_02750"
     CDS             7560..7967
                     /locus_tag="PKM34_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNQ82532.1"
                     /translation="MPSIFTRIVNGEIPCYKVAEDDHFLAFLDINPLAKGHTLVIPKK
                     EVDYIFGVEDNMYAGLFLFAKRVALAVEKVVPCKRMGIAVLGLEVAHAHIHLVPINTV
                     YDIDFSKPKLVLQKEEFLELVEKISAEFSKGAQ"
BASE COUNT         2189 a         1980 c         2035 g         1934 t
ORIGIN      
        1 caggacgaaa acggcaatta tacccgtgaa attggagcct atgaacctaa ttacatggct
       61 ttctacctgc aggataaatt cgcattccgc gatctgatct ttaacgtggg aatccgtgcc
      121 gaccgcttcg atgccaacca gcagatgctg aaggatccgt tcctggttta ccaggcctat
      181 acggtgaagg aagtttcgtc ggtcggtgga gatgccgtct cgcatccgtc caatatggga
      241 agcgactatg ttgtttatgt cgataatgtt aacaatccca caagggtagt cggctaccgc
      301 aatgagtacg actggttcaa cgcctccggt gctgaaatcc aggacccgaa cagtcttgat
      361 gtcggctcag ggatctctcc ttacctggtc gatcctacac tggagcggcc taccattgct
      421 gcattcaaag attatgaacc ccagtggtcg gtcatgccgc gtatcgcctt ttcattcccg
      481 atctccgatg aagcgctgtt ctttgcacat tatgatgtgc ttacccagcg cccgacaaac
      541 agtgttttca gcgacccttc cacgtactac ttctttaata atgtatccgg tacgatcaat
      601 aacccgggtt tgaaaccgac acagactatt gactatgaag tgggtttcca gcagaaactg
      661 accaattcat catcggtgaa actggttaca ttctatcgtg aaatgcgcga catgctccag
      721 atctaccgtt tcaacggagc ctatcctaaa gattatacct cttttaataa tatagacttt
      781 ggaacggtta agggtcttac cgtgcagtat gacctgagaa agaccggaaa cgtcaggttg
      841 agcgcctatt ataccttgca gtttgccaat gccaccggtt caaaccaggg aacaacggca
      901 gcccttgtcg cctccggatt acctaacctc aggagtacct tcccgatcca gaccgaccgc
      961 cgccactcct tcaacatttt cctcgattac cattttggcg gggatgctga atataatggc
     1021 ccggtcagcc agcgtaaaaa atcaggtaaa cctcccattc agtggttgag ctatttcgga
     1081 tttaccgtta ctcttaacgg aggatcggga acgccgtaca ctcagtcgag gaatgtggtc
     1141 gggataaacc aggcaggtac caacctggtg aaaggaactt actacggcgc acgcggcccc
     1201 tggcagttca ggaccgatgc cacgatcgac aaggatttcc attttatgct gggtaacggg
     1261 gaaaaccaac gccctgcctt tgtcaatgtt tatctgcgta tcaataatat ccttaaccag
     1321 aaaaacatca tgggcgtata tccgttcacg ggcaatccgg atgatgacgg ttatctgtcg
     1381 gctcctgaat cccagaagca gatcagtgaa gcaacaagtg agcaggctta ccgcgatttg
     1441 tatgccattg cagttaataa cccgggcaat tacagcagcc cgcggacgat caggctcggg
     1501 gttatcttca acttttaatg cttaggaacc attcttcaat tgataatatt aagaattata
     1561 atatgaaaat gagaattatc tatcggttac tgtccgttgt gctgatcgga ttgctcctga
     1621 caggcggcgc ctactccagg gaattcgtcg gggcaaagaa aaatgccggt acaattaaag
     1681 gaacggcagc cgggtgttca gccgcttcag gctttcgttt cctggatata aacaatatca
     1741 gggctcgtat caacaccggc ggagatatgt ggtgggacct tccgggagga atcggagccc
     1801 aatattatat tccgaaaacc ggaaccgcta cctccatgtt ttccggatca ctctggatcg
     1861 gaggactgga tatcaacaac cagcttaaac tggctgctat ccggtaccgc caggtaggta
     1921 acgattactg gccgggaccg ctgacaaccg acggaaccgc ggcggtggac caggaaacct
     1981 gcgcaaaata cgacaagcat ttcctgatca cccgactgga agtagacgaa ttcctcggat
     2041 ggtggaacag cacgaataaa gcggaggagt atccgggcta cagtattccc aaatctattc
     2101 tggaatggcc ggcccacggc gatgtagcca aactgaacag ctattacctg gccccgttcc
     2161 gggatgttga cggcgatgga gaatatgacc cgaataacgg cgactatccg tactatgaca
     2221 ttaccaatga actttgcccc ataaattttg ccggcgatcc gagttatatc ccggcaagga
     2281 ccatggaaga ggatctgacg gaatataaag ttgcgctgac aggcagtatc ctggttgacc
     2341 aggtcatcaa aggcgaccag acgctttggt gggttttcaa tgataaaggt aacacccata
     2401 cggaaacaca gggcgccccg atcggaatgg aagtaagagc ccaggccttc ggttttgcga
     2461 ccaacgatga aatcaacaat atgtcgttct attcctatga agtgatcaac cgttcaacct
     2521 ttgaactgac caacacctat ttctgcccct gggtagatac cgacctggga tatgcatggg
     2581 acgactacgt aggttgcgat gttcttcgcg gattaggcta ttgctataat ggtgttgcga
     2641 tcgacggcag cggtgagccg gaatcgtacg gtgcgcagcc cccggctatc ggcgtcgact
     2701 ttttccaggg cccgtacata gatccggatg gattcgacaa tcccagattt acgggagatt
     2761 gcagcatcct tggccaggga ccgaattttg aagtcgacca gatggccatc aatggcgtaa
     2821 acttcggcaa cggaatcgtg gatgacgaac gttacggaat gcgccgtttt gtttaccata
     2881 ataatacctc ttccaacccg gcaactactg acccgggagt agcttccgaa tactataatt
     2941 tcttaagggg catatggaag gacaattcat tgatgttata cggtggaacc gcgcataaca
     3001 tagatgcggc tgctgtggga cctatttgcg aattcatgtt cccgggcgat tccgatccct
     3061 gcaactgggg gacgaaattc cagccaccaa acggcggata taaccagaac gggaaatact
     3121 ggacggagga agaatcgggc aatgagccgg gagaccgccg ttttatgcag tcagccggac
     3181 catttacgct gaaacccggc gctgttaact atatcacggt cggtatcccc tgggcaaggg
     3241 cagcatccgg cggcccctgg gcttccgttg aactgctgcg ggttgtggat gacaaatgcc
     3301 aggcactgtt cgacaactgc tttaaagtga tcgacggccc cgatgcaccg gaccttatta
     3361 tccgggaact tgaccgggaa ctgatcgttt tcatcgataa tcccaaaggc tccaacaatt
     3421 accttgaaca atacgccgaa ttcgatccga atattatcca gccaaatccg gcaaatagcg
     3481 aactcagaag cgattccata taccggtttg aaggatatca gatcttccag ttgaaagatg
     3541 cctccgtaac ccttggagat gctaaagacg aatacgggaa tttcaatcct gacatggtca
     3601 gactggttgc ccagttcgac gttaaaaacg gtgtaggcag gatcatcaac cattatttca
     3661 acaaagctat cggattcaat atccctgttg ttgaagtgga aggaggcgat gaaggaattc
     3721 agcactcttt ccggatcacc gacgacgcat tcgcatcagg cgacaagcgg cttgtcaatc
     3781 acaagcaata ttattatacc gttgttgcat atgcttacaa tgagtatctg ccttacgacc
     3841 cggaagatgt catcgggaaa atgggccaga aaaaagctta cctgcccgga aggaaaaata
     3901 ttgtcaccta tacggccatt ccacataaga ccatcaacgg cctggtaatg aattccacat
     3961 acggcgatgg cccggcgata acccgtatcg aaggacaggg caatgatgga atgatcctcg
     4021 acctcacacc ggaaaccatt gaggagatcc tgagcaaaca accggttgga accgtgatcg
     4081 acggcgatac tgtcgatatc ggccatccct tgtatccgat tgcttatgaa gccaaatatg
     4141 ttaatggatt tggcccactc aacattaaaa ttatcgatcc gttgaatgtt cccacaggaa
     4201 agtattacgt caggtttgat accctttaca ccatgcggaa cttcaatgtg acgggcaaca
     4261 cagctctaat tgcgggagga gatacggcaa gctatattac aggcagctgg actctggtcg
     4321 actcgttggg cagggaattc aagtcggatg ccacccttga caagcagaat gagcaattat
     4381 ttctcgactt cgggatctcg atcaacatga agtcgttcta taccccgggg cccaagaagg
     4441 tgggtcagaa accggatcag aacggcccga tcacgattta cgaagtgctg gccgataaca
     4501 acggtgtact tgagtcgtca gtgacttacc aggacagctc ctatatctgg cttggtggag
     4561 tcgtagataa cgacgtcccc aatgatccgc gtaactggat acgggccgga acttaccagg
     4621 atggaaattc atccgactgg aacatgcctt cggcttcatt tgatcccaac cagaacttcg
     4681 agcgcctggc cggcggaacg tgggccccgt ttgttctcac agcagctaac gaccaggata
     4741 taaatggacc ggcttacagc attgcctgta aagaaaggac atttatggag gatgttttca
     4801 gtgtcgatat cgtatttaca ccggataaat cgaaatggtc aaggtcattg gtcattgaga
     4861 tgtgccccga tgctaacctg tcacagggaa aggcggcacg gttccagttc agaagggctc
     4921 cgtccatcga taaagacgga aatccggctt cgggcggcag cggccccagt tcagatccca
     4981 atgcagcgaa ctacgtgtca gacagcagta tgagctggtt cccgggttat gccatcaatg
     5041 tggaaaccgg tgaacgactg aatattgttt tcggtgaaga cagctggctg gtagcagaca
     5101 acggacggga tatgaagtac aacccgactt caaccatttt tgatcccgta tcaggcaatg
     5161 cgcgtttcgg cggtaagcac tatgtgtata tcatggatca tgtcagaagg gatctccagg
     5221 gaagaatctt tgatttcccg gcatacgacg gctgcaaata catccggaac ggatatggtt
     5281 tgaacgacct gccgccgttc ctgtatgaac tggcgtggat gtccaccgcg atgtgggtcg
     5341 gtatgccgct atccattgac ggaaaggaat ggctgaacaa tgaagtgaag atcaggatca
     5401 gggtagccaa accctacaaa cgtttctact cgacaacatt ggattctgct tctattgcca
     5461 attctcagaa cggccacatg ccgatgtacc agttcgaaac ccaatcggtg gcaacacagt
     5521 tgagcgaccc cgaaaaagcc aagagtgacc ttgacctgat ccaggtggtg ccgaatcctt
     5581 actatgctta tgcaggcggt gaaggatatg agcggaatgc tctcgacaac aggatcaaga
     5641 tcaccaacct gccggaacgt tgcacgattt caatctacaa cgtaaacgga acgctgatcc
     5701 ggcagatcac aaaggatgag cctaaaacct cggtagactg ggatctcaag aactttgccg
     5761 gtgtaccgat cgcaggcggg gtctacatca tccatgttaa agccgataat ggggagaaaa
     5821 tcctgaaatg gttcggcggg ctcagggtac ccgatcttaa tgtattttaa tcagagacca
     5881 ggataaaata acaatattaa tttcatccac tatgaagaat ctttacagat acctggcagc
     5941 cttgacgatg ataacggcat tgttctttgc cactacaggg gtgcaggctg gaaataaaga
     6001 ccgatccgga caggcaggtg cgcctgaact gctgatcaat ccctggacag gcagcgccgg
     6061 ctggggagga gtgaatacct ccaatatcag gggcgttgaa tcgatgttcg caaacattgc
     6121 aggactggcc tttgtcaata aactcgagct tggattttca cagacctact ggctgaaagg
     6181 gtccggtatc ggaatatcga acttcggtct cggcgtcagg gccggtgaaa ccggcgcgat
     6241 agggatttcg gtgttctcca tgagatttgg cgatctggac gtaacaacga ctgaacagcc
     6301 cgatggaacc ggtgctactt acagtcccag cctgctgaat tttgcccttt cctattcgaa
     6361 gactttttct aacagcatat acgccggtct tacgatccgc atcatttcgg aatctatccc
     6421 ggatgccagc gcacagggta tttgccttga tgctggcgtt atgtatgtga caggggaaag
     6481 agaccaggcc aaattcggga ttgctttgag aaatctcggt ccgacaatga agttcaccgg
     6541 cgacggattc tccatcaaag ctttcttcca gggcaatgac aattcgatct cggtcatcca
     6601 gcgctcagaa tcatttgagt tgccaacaca gcttcgcatc ggggcatcgt atgactttca
     6661 gatcgcggcc cttcaccggc tgacactggc aggaaatttc acttccaatt cattttcaaa
     6721 agaccagttt accctcggcc tggaatactc gctgaatgag atcctgatgt tgcgcggagc
     6781 ctacacatat gaagacggta tattggaaga tgtgtctgat ccaagccgga cgaatgcata
     6841 taaaggcctt agccttggcg cttccgtaga agtgccgctc agcaaaaaga ccgatctgaa
     6901 actcggcatc gattatgctt atcagtcgac cgataatttc aagggaacac aggctttcgg
     6961 gatccgaatc aagtactaaa catttaaaat tataccgtat ctttgtgcgt tcaaagagac
     7021 ccttttatgg aagggtctct ttgttattaa aacattttac agggattgaa tcatggcaga
     7081 accaaagtat tatacaaaag aaggtcttga taagctgcgg gcagagcttg atcacctgac
     7141 aacagtggaa cggccttcta tctcaaaaca gattgccgag gcgcgtgaca aaggagatct
     7201 ttcggaaaat gccgagtacg atgcagctaa gaacgcccag ggaatgcttg agatgaaaat
     7261 agccaggctg caggaagaga taagcaacgc ccggatcatc gacgagtcga aaatggatct
     7321 gaataagatt ctgatcctga gtactgttaa actatcgaac ctgaagaacg gggccgttat
     7381 gtcgtatacc attgtgcctg aatcggaagc aaatttaaaa gccggaaaga tttccgtatc
     7441 ttcgccgatt gccaaagggc tgctcggtaa aactgtcggt gacaaagtgg agatcgacgt
     7501 gcccgccggt aaactgaact tcgagatcat cgaaatttca cgttcgtaaa cattatacca
     7561 tgcccagtat ctttacccgg atcgtcaacg gggagattcc ctgctacaag gtagctgaag
     7621 acgatcattt cctggctttc ctggatatca accccctggc caaagggcat acgctcgtta
     7681 tacccaaaaa agaggtcgat tatatctttg gggttgaaga taatatgtat gcaggcttat
     7741 tcctatttgc aaaaagggta gcccttgccg tggaaaaagt cgtaccctgc aaacgaatgg
     7801 gtatcgccgt tttaggcctt gaagtggccc atgcccatat tcatctcgtt cccatcaata
     7861 ccgtgtacga tatcgatttc agtaaaccga agctggtgct acaaaaagaa gagtttcttg
     7921 aactggttga aaaaatcagt gcggaattct ccaaaggcgc tcaatagggc ctgatatctc
     7981 ggccttcgat gtaattgatg aacgctttgc acgcggcttt gtttccgccc ggtgtaggat
     8041 aattgccggt gaagtaccag tcgcccgtat ggttcgggca ggcggcatgc aaatcttcta
     8101 tgttctggta aacgacctgg atgacggcct ttgtttca
//
LOCUS       DAOQXS010000056         8064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9415_length_8064_cov_3.918966, whole genome shotgun sequence.
ACCESSION   DAOQXS010000056 DAOQXS010000000
VERSION     DAOQXS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_8064_cov_3.918966"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2796)
                     /locus_tag="PKM34_02755"
     CDS             complement(<1..2796)
                     /locus_tag="PKM34_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNQ82533.1"
                     /translation="MNKSILLFLIIAISCTTMGQKSYEKEWKEVEAAVRKGLPQSALK
                     IVDSVYLDARSSDNAPQFLKATLYQIKLRSDFQENFMESSILQVETELKTASVPVKQI
                     LHSIRAELYWRYYQGNRYRFMERTGVSNPDLTDIKTWDLKTLVNTVTESYLASVGQEY
                     ATLKIKLDFIDPILETEPGSKIYRPTLYDFLAHRALEYFANEEPAVVRPAEGFLLDQE
                     NYFSPAGEFSSLKITSPDENDLKWRALLIYQNLLRFHSGDDDPSALIDADLARLKFVQ
                     QHSVLQDKDLLYKNALAELQEKHKSAPGAADVAYELAASYNLSGTFYVRNQGEVNRWD
                     LKKAREICEKTIRDYPGTDGAKNCAILLAEISEPSLNFSVNYANVPDQPFLGSLNFRN
                     VSTVYFRIVKADPEEDRNRRQREMGENLVAKYLALQPVKAWSVKLPDDGDFQIHNTQV
                     RIPSLPKGYYVILASDNEGFSCENRPVAYSPAWVTGISYVSQRNPEDGKLEIYVLDRD
                     KGIPMKGVSVAAYIREYDYKTRSYLDKLINTLTSDENGYISMAAVRNDSKSFFLKFSQ
                     GNDLYFTENYFYLYPTGPTEDTRITTYFFTDRSIYRPGQTVYFKGIVLERTGTKSEIK
                     PGYSLVVTFYDVNNQKVSEKAVTTSDFGSFNGIFTAPAGGLTGEMSIRCETGSVGFSV
                     EEYKRPRFKVEVDPLEGSYKLNETVNVTGKALNYSGSAVDQASVSYRVVRTARFPVWR
                     SWWRWFPPTPETEITSGKTLTGADGSFSLSFKALPDLTVDRKYQPVFNYTVYVDVTDI
                     TGEVRSAEGSLSVGYQAMLLDAGIPDEVDQLGPDAFPLSATNLNRQPVPAEVKVSVHL
                     LDAPARLIRKQQWENPDVFTMTREEFLASFPYELYDDENDPETWNKKELMMEKSYRTP
                     SDSTIIINNLKSWKEGHY"
     gene            complement(3015..3938)
                     /locus_tag="PKM34_02760"
     CDS             complement(3015..3938)
                     /locus_tag="PKM34_02760"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNQ82534.1"
                     /translation="MFQKSVISNDGLTLFLAPTTKAVGSYGFMKVGTDGFSRRTTKKS
                     LGLRLSLGFLFLFISNQLISQDTFRKVENNTFQHGEFLKYRVFYDSWVTYWMTAGYGT
                     MEISPEPVKINGRDTYHITVNGNSAGLFNVFYKVRDKFETYMDKEGLMPLKFIRYTRE
                     GGYKKDDTIFFDHQARKAVSIRKTKDITQYVQDIVSAFYYVRSWDFDTAEVNDTYYVD
                     FFLDDSLYHSEIVFMGRESVRTDFGTLPCMRFKPRVAVGELFQDPYPMELWVTDDRNK
                     IPVLMKSAVFIGSVKIELVEYRGLRWPFESH"
     gene            complement(4019..6868)
                     /gene="leuS"
                     /locus_tag="PKM34_02765"
     CDS             complement(4019..6868)
                     /gene="leuS"
                     /locus_tag="PKM34_02765"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ82535.1"
                     /translation="MEYNFNAIEKKWQDYWRENKTFKAFNQSGKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLQGFNVLHPMGFDAYGLPAEQYAIQTGTHPAITTEK
                     NIARYREQLDQIGFSYDWDREVVTSDPFYYKWTQWTFIRLFKSWYNRESNKAEQIDKL
                     VNIFEQEGNANVKAVCNPTDTFTAESWKAMTEKEQQQILMNYRLAYLSDTWVNWCPAL
                     GTVLANDEVKDGFSERGGHPVERKQMKQWSLRITAYAQRLLDGLEKLEWSDSLKEVQR
                     NWIGRSEGAAVYFQLAAGSRQPAAGSWQSNMKPGTRNPEPGAFIEVFTTRPDTLFGAT
                     FMVLAPEHELVPQITTPDRKEKVAEYVEWAKNRSERDRMTEVKRITGEFTGAYGINPL
                     TGEQIPIWVADYVLAGYGTGAIMAVPAHDSRDFAFARHFDLPIIQVVKKEDEEESDPL
                     LWSESYDAKDGVMVNSGFITGLKVKAAITATIRKIEEQGVGYGTVNFRLRDAIFSRQR
                     YWGEPFPIYYRDEMPYPLDESRLPLELPEVDKYLPTETGEPPLARAKNWHTPEGYPLE
                     TSTMPGFAGSSGYYLRYMDPWNNDAYFSKESNRYWQDIDLYVGGDEHAAGHLIYSRFW
                     NMFLFDIGLVCKEEPFKRLINQGKIQGRSSFVYRINGTSKFVSYGLKDQYDTTRLHVD
                     IEMVDNDVLDIEAFRNWRAEYRHAEFILENGKYICGWEIEKMSKSKHNVQNPDDLIVR
                     YGADTLRMYEMFLGPVELSKPWDTKGIEGVFRFLKKYWRLFFTEQGEFQVTDETPTAA
                     ELKILHKTILKTKEDIERFSFNTTVSSFMICVNELTDLKCNKRSILEPLNILISSFAP
                     HIAEEIWSLLGHDGTIAYASFPEFDEEFIRENTFNYPVSFNGKLRFQLELPVDFAPAD
                     VEAAVMAAPEARKWLAGGAPKKVIFVPKKIINVVV"
     gene            7109..7987
                     /locus_tag="PKM34_02770"
     CDS             7109..7987
                     /locus_tag="PKM34_02770"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNQ82536.1"
                     /translation="MSKMEERYHRRRYQSSVATTIVSITLVLTMLGLLGLTILHARKL
                     SDYVKENIGFRVYLKEDAATEDIIILQKKLDAEPFVKSSRYISPEEAARELTADLGED
                     FIDFLGYNPLPPSIDLRVRAAYANVESLETIERNLMQEVVVKEVFYQKSLVHLINKNV
                     NRISIVLLGFTGLLMLIAIALINNTIRLSVYSKRFIIRTMKLVGATRRFISRPFIVRG
                     VLQGFYSAIFAIILLVTILYFLMQQVPELVNLFDLYLYLAVFFLVMLTGMFLAWVSTW
                     FAVRKYLRMTEDELYS"
BASE COUNT         1888 a         2025 c         2003 g         2148 t
ORIGIN      
        1 gtaatgacct tctttccagg attttaaatt atttatgatg atggttgaat cggatggtgt
       61 ccggtacgat ttttccatca tcaattcctt tttattccat gtttcgggat cattttcatc
      121 atcataaagc tcatagggaa aactggccag gaattcttcg cgggtcatgg taaacacatc
      181 gggattttcc cattgttgtt tccggatgag gcgtgcaggg gcatccagca gatgcaccga
      241 cactttcact tcggcaggca ccggctgtcg gttgagattc gttgcgctga gcgggaatgc
      301 atccgggccg agctggtcca cttcgtcagg gattccggca tcgaggagca tcgcctggta
      361 gccgaccgac aggctccctt cagctgagcg cacctcgccc gttatatcgg tcacatcaac
      421 ataaaccgta taattgaaaa ccggctggta tttcctgtcc accgtaagat cgggcaacgc
      481 cttgaacgac aggctgaacg atccgtcggc cccggtcagg gtcttgccgc tggtgatctc
      541 cgtttcggga gtgggcggga accagcgcca ccaggagcgc cacaccggga agcgtgccgt
      601 tctgaccacc ctgtaggaaa ccgaagcctg gtcgacggca ctgccgctgt aattcaaggc
      661 tttccccgtc acgttcaccg tttcattcag cttgtagctg ccttccagcg gatcgacctc
      721 caccttaaag cggggccttt tgtattcttc taccgaaaat cccacgctgc cggtctcgca
      781 ccgtatgctc atttcaccgg tcagccctcc ggcaggggcg gtaaaaatgc cgttgaacga
      841 accgaaatcg cttgtcgtaa ccgctttttc cgataccttc tggttgttga catcataaaa
      901 agtgaccacc agggagtaac cgggcttgat ttcgcttttc gtcccggtcc gttccagcac
      961 gatccctttg aaataaacgg tttgtccggg cctgtagatg gaccggtcgg tgaaaaagta
     1021 ggtagtgatg cgggtatcct ctgtcggtcc ggtgggatag aggtagaaat agttttccgt
     1081 aaaatagagg tcgttcccct gggaaaattt cagaaaaaat gactttgaat cgttcctgac
     1141 agcggccatg gaaatataac cgttttcatc ggaagtgagc gtgttgatca gcttatcaag
     1201 gtatgagcgt gtcttataat cgtattccct gatgtaagcc gccacggaaa cacctttcat
     1261 cgggattcct ttgtcgcggt cgaggacata gatctccagt ttcccgtctt ccgggttgcg
     1321 ctggctgaca taactgatgc cggttaccca tgccggggag taggcgacgg gccggttttc
     1381 acaagaaaat ccttcattgt cggaagccag gatcacgtag taaccttttg gcaacgaagg
     1441 tattctgacc tgggtgttat gtatctggaa gtcgccatca tcaggcagtt tcaccgacca
     1501 ggctttgacg ggttgcaagg ccaggtattt cgcgacaagg ttctcgccca tttcccgttg
     1561 ccggcggttc ctgtcttctt ccggatcagc tttcacaatc cggaaataga ccgtactgac
     1621 attcctgaag ttcagggagc cgaggaatgg ctgatcgggc acattggcgt agttcaccga
     1681 aaagttcaat gaaggttcgc tgatctctgc aaggagtata gcgcaattct ttgccccgtc
     1741 ggttcccgga taatccctga tggttttttc gcagatctcc cgggccttct tcagatccca
     1801 gcggtttact tcgccctggt tcctgacata gaacgtcccc gagagattat acgatgcagc
     1861 cagctcgtag gccacatcag cagcgccggg agcggatttg tgcttttcct gcaattcggc
     1921 cagggcattt ttatataaga ggtctttatc ctgaagaaca gaatgctgct gcacgaattt
     1981 caacctggcc aggtccgcat cgatgagggc ggagggatca tcatcgcccg aatgaaaccg
     2041 cagaagattc tgatagatca ggagggcgcg ccatttcagg tcgttctcgt cgggcgacgt
     2101 gattttcagg gatgagaact caccggcggg ggagaaataa ttttcctggt cgaggaggaa
     2161 accttcggcc ggtctgacca cggcgggctc ttcattggca aaatactcca gcgcgcggtg
     2221 ggcgaggaaa tcatacagcg tcggacggta gatttttgaa cccggttccg tttcgaggat
     2281 cggatcgata aaatcaagtt tgatcttcag ggttgcatat tcctggccga cggatgccag
     2341 gtagctttct gtaaccgtat tgacgagggt tttgaggtcc caagttttga tatcggtcag
     2401 atcagggttg gaaacccctg tgcgttccat gaagcggtag cggttgccct ggtaatagcg
     2461 ccagtagagc tcggccctga tcgagtgaag tatctgttta acaggaacag aagcggtctt
     2521 cagctcagtt tcaacctgca ggatgctgct ttccataaaa ttttcctgga aatcggatcg
     2581 aagtttgatc tggtataagg ttgctttgag gaattgcggg gcattatccg acgatctggc
     2641 atccaggtaa accgaatcca caattttgag ggccgattgc ggcaggcctt tgcggacagc
     2701 ggcttccacc tccttccact ctttttcata agacttttgc cccatggtag tacaacttat
     2761 ggcaatgatg agaaataaca gaattgattt attcatggct aaattgatta ttcggttggt
     2821 tcttctttat gttttttcga atataaatat catttcacga agttacatga agaagacatg
     2881 aagttacatg aaggataagc taaatttatg ccaaagtttt ttataacatc cgtacagcac
     2941 aatacaacct aataacgcga gcgcagcgag ctattgacta ctttatgacc actttatgac
     3001 gcgagcgcag cgagctaatg actttcaaac ggccatcgca accctctgta ttccaccagt
     3061 tcaatcttca ccgatccgat aaaaacagcc gacttcatca aaacgggtat tttattccgg
     3121 tcgtcggtca cccatagctc catcgggtat ggatcctgga ataactcacc cacagcgacg
     3181 cgtggtttga accgcatgca gggcaaagtc ccgaaatcgg ttctgaccga ttcacggccc
     3241 atgaaaacaa tttcgctgtg ataaagggaa tcatccagga aaaaatcgac gtaatatgta
     3301 tcattcacct cggccgtatc gaaatcccat gatctgacat aataaaaagc agatactata
     3361 tcctggacat actgtgtaat atcttttgtt ttgcggatac tcaccgcctt ccgggcctgg
     3421 tggtcaaaaa agatcgtatc gtctttttta tagcctccct cccgggtata gcggataaat
     3481 ttcagcggca tcagcccctc tttatccata taagtctcaa atttatccct gaccttgtaa
     3541 aagacgttga aaaggccggc agaattgcca ttgacagtga tatggtaagt gtccctgccg
     3601 tttattttca ccggttcggg agaaatttcc atcgttccgt acccggcggt catccagtag
     3661 gtcacccagg aatcgtaaaa gacgcggtat ttcaggaatt cgccgtgctg aaaggtgttg
     3721 ttttcaactt ttcgaaatgt atcctggcta ataagctggt ttgaaataaa aagaaataaa
     3781 aatccaagag ataatcgtaa tcccaaactt ttcttggtag tccgacgact aaagccgtcg
     3841 gttcccacct tcataaatcc ataggaacca acggctttag tcgttggagc aagaaataat
     3901 gtcaggccat catttgaaat aactgacttt tgaaacaccc tcactactaa taaaacaggc
     3961 tgattagatt tgtgaaatac cttcccgaca ggtcgtatta taatctggaa cctggaactc
     4021 ataccactac atttattatt ttcttcggca cgaagatcac ctttttcggc gctccgccgg
     4081 ccagccactt ccgggcttcc ggtgcagcca tcacagcggc ttcaacatcg gccggtgcga
     4141 agtcgaccgg cagctcgagc tggaaccgca gcttaccatt aaacgaaacg ggataattaa
     4201 acgtattttc ccggatgaat tcttcgtcaa attcagggaa agatgcataa gcaatcgttc
     4261 cgtcatgacc cagcagcgac cagatctctt cggcgatgtg gggggcaaaa gatgagatga
     4321 gtatattcag cggctccagg atcgaccgtt tattgcattt cagatcggtg agttcgttca
     4381 cgcagatcat aaagctggaa acagtcgtat tgaaggaaaa ccgttcaata tcctctttgg
     4441 ttttaaggat ggttttatga aggattttga gctcggcagc agttggagtc tcatcggtaa
     4501 cctgaaattc accctgctct gtaaagaaca accgccagta tttcttcagg aaccggaaaa
     4561 ctccttcgat ccccttggta tcccagggtt tcgacagttc caccgggccc aggaacatct
     4621 cgtacatccg gagcgtatcg gcgccgtaac ggacaatgag atcatcgggg ttctgcacgt
     4681 tatgctttga tttcgacatt ttttcgatct cccatccgca gatatatttc ccgttttcca
     4741 ggataaattc ggcatgacgg tactccgctc tccaatttcg gaaagcttcg atatcgagga
     4801 catcgttatc caccatttca atgtcgacat gaaggcgggt cgtatcgtac tgatctttaa
     4861 ggccataaga aacgaactta ctggtcccgt tgattcggta gacaaagctt gaccgcccct
     4921 ggatcttccc ctggttgatc agccgtttga atggctcttc tttgcatacc aaacctatat
     4981 cgaagaggaa catgttccag aagcgggaat agatcaggtg gccggcggcg tgctcatcgc
     5041 caccgacgta cagatcgata tcctgccagt acctgttgga ttccttagaa aaataggcat
     5101 cgttgttcca gggatccatg taacggagat agtagccgct ggagccggca aatcctggca
     5161 tagtactggt ttccagcgga taaccttccg gtgtgtgcca gtttttggcc cgtgccaggg
     5221 gcggttcgcc cgtttcggtg ggcaggtatt tgtcgacttc cggtaattca agcggaagcc
     5281 ggctttcgtc gagcggataa ggcatttcgt cgcggtagta aatcgggaaa ggctcacccc
     5341 aatagcgctg gcgactgaaa atcgcatcgc ggaggcggaa attcaccgtt ccgtatccaa
     5401 cgccctgctc ttctattttg cgaattgtgg cggtaatggc cgcttttact ttcagtccgg
     5461 tgatgaagcc cgaattgacc atcaccccgt ctttggcgtc atacgattct gaccagagca
     5521 acggatcgct ctcctcctca tcttcctttt taaccacctg gatgatcggc aggtcaaagt
     5581 gacgggcgaa tgcgaagtcg cggctgtcgt gcgccggaac ggccatgatg gctcccgttc
     5641 cgtaaccggc cagcacgtaa tcggccaccc agatcggtat ctgttcccct gtcagcgggt
     5701 tgatgccgta agctcccgta aattcgccgg tgatccgttt cacttcggtc atccggtcgc
     5761 gctccgagcg gtttttcgcc cattcgacat attcggccac cttctctttc ctgtcgggcg
     5821 tggtgatctg cggcaccagt tcgtgctccg gtgccagcac catgaatgtc gcgccgaaga
     5881 gggtatcggg acgggtggtg aagacctcta taaatgctcc aggttccggg ttccgggttc
     5941 cgggtttcat atttgactgc caactgccgg ctgccggctg ccgactgccg gctgccaact
     6001 gaaaatacac tgcagcgcct tccgaacgcc cgatccagtt gcgctgcacc tctttcaggg
     6061 agtcgctcca ttcgagcttt tccagcccgt cgagcaggcg ttgtgcgtaa gccgttatgc
     6121 gcagcgacca ttgtttcatc tgtttgcgtt ccaccgggtg gccgccgcgt tccgaaaaac
     6181 cgtctttcac ctcgtcgttg gccagcaccg ttcccagggc cgggcaccag tttacccacg
     6241 tgtcggaaag ataagccagg cggtagttca tcagtatctg ctgctgttcc ttttcggtca
     6301 tggctttcca tgattcggcc gtgaatgtat ctgtcggatt gcaaaccgcc ttcacgttcg
     6361 cgtttccttc ttgttcaaaa atattaacga gtttatcaat ttgctctgct ttgttacttt
     6421 ccctgttgta ccacgacttg aaaagccgga tgaaagtcca ctgcgtccat ttgtaataaa
     6481 acgggtcgct cgtcaccact tcccggtccc agtcgtacga aaagccgatc tggtcgagtt
     6541 gctcacggta gcgggcaata ttcttttcgg tcgtaatggc cgggtgggta cccgtctgta
     6601 tggcatactg ttctgccggc aggccgtaag cgtcgaagcc catcgggtgg agcacgttaa
     6661 atccctgcag gcgtttgtag cggctgtaaa tatcggatgc aatgtaaccc agcggatggc
     6721 cgacatgcag tccagcccct gaggggtaag ggaacatgtc gagcacataa tatttcggtt
     6781 tgccagattg attaaaggct ttgaaggttt tattctcccg ccagtaatcc tgccattttt
     6841 tttcgatcgc gttaaagttg tactccataa ttgttccagt gttccagcct aaaagtttat
     6901 tatttaacca caaaggaaca caaagagtta cacgaagtca cacaaaggtt ttgaatttgt
     6961 aactgtaact ttgtggccct ttgtgaagta ctttgtgcgc ctttgtggtt aagtcctttc
     7021 aaatctttgc caaagaggct atccaggcag caaatttaca aataatcacc cgatgcagag
     7081 tccgtgaaaa agtgctatct tcgtcgccat gtcgaaaatg gaggaaagat accaccggag
     7141 gcgctaccag tcgagcgtag ccacgacgat cgtcagcatc acgctggtgc tgacgatgct
     7201 gggtttgctg ggactgacga tacttcatgc ccgcaagtta tcggactatg tgaaagagaa
     7261 tatcgggttc agggtgtacc tgaaggagga tgctgcgacg gaggatatta tcatcctgca
     7321 aaaaaagctg gatgcggaac ctttcgtcaa atcctcacgg tacatctccc cggaggaggc
     7381 tgcccgggaa ctgaccgccg atctgggcga agacttcatc gatttcctgg gatacaatcc
     7441 gctgccgcct tccatcgacc tgagggtaag agccgcctat gccaatgtgg aaagccttga
     7501 aaccatcgaa cgaaacctga tgcaggaggt ggtcgtcaag gaggtttttt accagaaatc
     7561 gctggtccat ctcatcaata aaaatgtcaa ccggatcagt atcgtcttgc tcgggttcac
     7621 cggtttgttg atgttgatcg ctattgcttt gataaacaac actatacgtt tatccgttta
     7681 ttctaaaagg ttcattatcc ggacgatgaa actggtgggt gccacccggc ggttcatcag
     7741 tcgtccgttc attgtcaggg gagttttaca gggcttttac agcgcaatct tcgccatcat
     7801 cctcctggta acgatcctgt atttcctcat gcaacaggtc cccgaactgg taaatctttt
     7861 cgatctctac ctttacctgg ccgtgttttt tctggtgatg ctcaccggta tgttcctggc
     7921 atgggtctcc acctggtttg ccgtcaggaa atatctcagg atgacggaag atgaactgta
     7981 tagttaatgc tgaattttta tacttttgtt ttaaattctt aaaagatggt ggaactgctc
     8041 ctgatattac ttcgtataat aagc
//
LOCUS       DAOQXS010000057         8063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9417_length_8063_cov_3.146853, whole genome shotgun sequence.
ACCESSION   DAOQXS010000057 DAOQXS010000000
VERSION     DAOQXS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9417_length_8063_cov_3.146853"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKM34_02775"
     CDS             complement(<1..912)
                     /locus_tag="PKM34_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNQ82537.1"
                     /translation="MAINLHPRNLRIEDYDYPLPDDRIALFPLEQRDSSKLLLVRNGR
                     TGEDIFSNMATHLPPDSLVVFNETRVIHARLLFRRPTGSRIELFCLEPVAPYRDHLRA
                     FGCTGQADWLCLVGNSKRWKHGGLELTAGEGKDQFVLKAETTSRNEDGSSVIRFTWHP
                     EHLTFSHILEQAGKIPLPPYIHRDPVHNDERTYQTIYASREGSVAAPTAGLHFSPEVL
                     ASLEAKGIKSQRLVLHVGAGTFRPVSSERLEGHEMHAEQVDLPLLALKDLRGNLDRKI
                     TAVGTTTTRLLESLYWHGVKVIMGLSEP"
     gene            1013..1738
                     /gene="pssA"
                     /locus_tag="PKM34_02780"
     CDS             1013..1738
                     /gene="pssA"
                     /locus_tag="PKM34_02780"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNQ82538.1"
                     /translation="MNFKKNIPNGITLLNLVSGGVSVIMALSGEPACAAWLILLAAVF
                     DFLDGLAARLLNARSAIGGELDSLADVISFGLAPSAILYALMLKSPNLPEFAAGEYQL
                     FPFSALLLAAFAAARLAKFNTDPGQAIEFRGLPTPATGLFVAAFPLMVHYQGDMEYVR
                     LLTGNFYLLLAVVIVLSALMVSSIPLMSFKFKDLSWKGNSYRYVLILSVPLLIIIFKF
                     MAIPLIIILYIILSIIRSKVKSQ"
     gene            1792..2979
                     /locus_tag="PKM34_02785"
     CDS             1792..2979
                     /locus_tag="PKM34_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNQ82539.1"
                     /translation="MLILSGIILLSGNITFAQDNEQKEGYKFTMVKELPSTPAKNQYK
                     SSTCWSFSSVSFLESELLRLGKGEYDLSEMFVVHNTYQAKAKGYARWQGNVNFAAGGA
                     FHDVLAVMETAGMIPEEVYSGKVIGEKEHIHNEMDAVLTGFMEALIKNDNGKLTPVWP
                     LAVKGILDAYLGEYPSSFKYKGKEYTPKSFAESLGLDLSQYVEIGSYSHHPFYEKFII
                     ELPDNWMMDEIYNVPLDEMMEIIDYSLDNGFTVGWGADISEKGFSWTNGVAVVPVVDL
                     EDMTGTEREKWEKLTQKEREKMIYSFEKPVPERVITQELRQTAFDNYQTTDDHGMHIT
                     GTAKDQNGQPYYYVKNSWGVTGNDYKGFFFASKPFVAYKTIDILVHKDGIPKEIRKKL
                     GLL"
     gene            complement(2981..3394)
                     /locus_tag="PKM34_02790"
     CDS             complement(2981..3394)
                     /locus_tag="PKM34_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNQ82540.1"
                     /translation="MKTTKKSGGSGFPTVFYDGSCHLCARSVRFILKYDRKKRFRFAS
                     LQSVTAKTLEIINLKDDSVILYEEGKILMKSQAAIRIAILLGFPWSVAGIFAVIPVSW
                     RDTIYDLIARNRHRWPGSSQYCEIGRSTPDDRILP"
     gene            3504..4874
                     /gene="icd"
                     /locus_tag="PKM34_02795"
     CDS             3504..4874
                     /gene="icd"
                     /locus_tag="PKM34_02795"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNQ82541.1"
                     /translation="MTGEKITKSGGRLNVPDIPVIPFIRGDGTGPDIWAASVRVFDAA
                     VEKAYHGKKRVEWLEVYAGEEAFNRFGEWLPQATIDTFRDYLVGIKGPLTTPVGGGIR
                     SLNVALRQLLDLYVCLRPVKYVTGVPSPVKHPERVDMVIFRENTEDIYAGIEWKSGSE
                     EVKKVIRFLEEEMKVKKIRFPETSGIGIKPVSEEGSKRLIRSAIRYALEENRHSVTLV
                     HKGNIMKFTEGAFKTWGYEVATQEFRDHVVTQRESWILGNIDKDPTISGENNARLIEP
                     GYYMMTPEQQQAIKDEVGEGMKLLPTHGNGQWKTKLMIKDSIADITLQQVLTRADEFE
                     VVATPNLNGDYLSDALAAQVGGIGIAPGANINYDTGHAIFEATHGTAPKYAGLDQVNP
                     GSVILSGAMMFKYLGWHEVHDLIWKGLEKAVSGKRVTYDFHRQMEGAVLLKCSEFGEE
                     IVENMK"
     assembly_gap    4956..5055
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5129..6442
                     /locus_tag="PKM34_02800"
     CDS             5129..6442
                     /locus_tag="PKM34_02800"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNQ82542.1"
                     /translation="MIGLKEKLRQKIEGWRPMTKRLVQEFGDVVIDEVTIEKVIGGMR
                     DLKVLITDISYLDPNEGIRYRSYTLNEVIEKLPKPKGAEMPYVEGVWYLLLTGDIPTE
                     EEVQEVVEEFQKRRILPRVIYEVIDAAPCCNHPMTTFASAILALHRESFFVKKYNAGI
                     PKSEYWDPMYEDAMNLLAKLCEIATYIYTKLYKDGKRIQSNPNLDMGANFAWMMGINP
                     PYDDVSRMHFILHADHESGNVSAHTGHLVASSLSDVYLSIAAMINGLAGPLHGLANQE
                     VLRWLQDLREQLGGDDPTEEDIRKYVWDTLHNGHVIPGYGHAVLRKTDPRYTIQREFS
                     LKHMPEDPLFKYTDLLYKVVPEILIQQGKAKNPWPNVDAQSGIIQWHYGVRQYDFYTV
                     LFGIGRAIGICANIIWDRALMYPLERPKSITTEMLEEIVGIKQKE"
     gene            complement(6470..7717)
                     /locus_tag="PKM34_02805"
     CDS             complement(6470..7717)
                     /locus_tag="PKM34_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82543.1"
                     /translation="MASLTQEQKFEFIRNLPMFSGADNKVLLELADSMSENEALKGSI
                     VIRKGDEADAVFIIAGGRVRVHDGNHVLARLEQGQMFGEYSLIDQRQRSASVTAEDNC
                     RLLRLDNRDFYRIAAGDPGILRGVLKELINRMRDMNELEEKLSRSYLKIQRQKELIEK
                     QNASIMQQKAMLEQQNFDLTKLNEEKNQLIGTVIHQIKNPLTSSLCMADMLDARDGFV
                     DETEREGLAIIRNSIKRINNLINEILDINAIESKVYELKMEPLNVREILEDLIRNYSY
                     FIDQKHINFKVQMVDITARLNRVYVTQIFDNLLSNAIKFTPDGKTIEVRLGENEDKIM
                     FCVCDEGPGMEKEKMEKVFDRYKRQTSMFDQESAGNGLGLAIVYKYATAMHGTVRCES
                     EPGSGTRFHVELPGREKTKGNKE"
     gene            complement(7744..8049)
                     /locus_tag="PKM34_02810"
     CDS             complement(7744..8049)
                     /locus_tag="PKM34_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HNQ82544.1"
                     /translation="MRGLVANTYFLLIRKFHMLPARSRRILPFMITQNWLVNYRDLDT
                     MQRVFNGMSRRINKPSGMENAIGDLVAGYSLYEAEFRGFFPEITDHINRFRSNLIGL"
BASE COUNT         2035 a         1884 c         2045 g         1999 t
ORIGIN      
        1 cggttcagac aaacccatga tcaccttaac gccatgccag taaaggcttt ccaggaggcg
       61 ggtcgtggtg gtgccgactg cggtgatctt cctgtccaga ttaccacgaa gatcttttaa
      121 tgctaacagg ggcagatcaa cctgttcggc atgcatttcg tgcccctcca ggcgttccga
      181 cgacaccggc cggaaggtgc ctgctcctac atgcaatacc agtctttgtg atttaatccc
      241 cttagcttct aacgatgcga ggacttccgg tgaaaaatgc agaccggccg ttggagcggc
      301 tacggaaccc tccctgctgg cataaatcgt ctggtaggtg cgctcgtcat tatgtaccgg
      361 atcccggtga atataggggg gaagagggat ctttccggct tgttccagga tatgagaaaa
      421 cgtcaggtgt tccggatgcc aggtaaaacg gataacggaa gatccgtctt cgtttcggct
      481 ggtcgtttct gccttcagga caaattgatc cttgccctct ccggctgtca gctccagtcc
      541 gccgtgtttc catcttttgg agttgccgac caggcaaagc cagtcggcct gcccggtgca
      601 tccgaaggcc cgcaggtgat cgcggtatgg cgccacaggt tcaaggcaga aaagttcaat
      661 ccggctgccg gtcggacggc ggaacagcaa ccgggcatgg atcacccggg tttcattgaa
      721 aaccaccagg ctgtcgggag gcaaatgggt ggccatgttg ctgaaaatat cctcaccggt
      781 acgaccgtta cgaaccagta acagtttcga cgaatcccgc tgctcaaggg gaaacagggc
      841 tatccggtca tccggcaggg gataatcgta gtcttcaatg cgcaggtttc ggggatgcag
      901 gttgatggcc acgggtctgt ccttaatttt tccacaaaga tataaaacag ttaatctttc
      961 gtacttttgc cgttagtcgg tatcaaggcc tgaggcttgc cgccctgcag cgatgaattt
     1021 taaaaagaat atccccaacg gaattacact cctgaacctg gtcagtggcg gagtctccgt
     1081 gatcatggcc ctctcgggcg aacctgcctg tgctgcctgg ctgatcctgc tggccgcagt
     1141 ctttgacttt cttgacggat tagccgcccg tttgctgaat gcccgatcgg ccattggcgg
     1201 cgaactcgat tcgctggccg atgtgatctc gttcgggctc gccccgtctg ccattcttta
     1261 tgcgctgatg ctgaaaagcc cgaacctgcc tgagtttgct gccggtgaat accagttatt
     1321 cccgttttca gcgcttctac tggctgcttt tgccgctgcc aggctggcaa agttcaatac
     1381 cgatccggga caggcgatcg agttcagagg attgccgacg ccggcgaccg gcctgttcgt
     1441 cgctgctttc ccgctgatgg tgcattacca gggggacatg gaatatgtca gattgttaac
     1501 cggaaatttt tatctgcttc tggctgtggt aatcgtgctg tcggcgctga tggtctcttc
     1561 catcccgctg atgtcattta aattcaaaga tttaagctgg aagggtaata gttaccggta
     1621 tgtactcatt ctctctgtac cgttactaat tattattttc aaatttatgg ctataccgct
     1681 gattatcata ctttatatca tcctatctat tatccggtct aaagtaaaat ctcaataata
     1741 tttattaacc taatcaggaa ttgtgttatg aaatcttttg cgttaaaatc catgctgatt
     1801 ctgtctggga tcatcctgct gagcgggaac attacattcg ctcaggataa tgagcagaaa
     1861 gagggatata aatttactat ggtaaaagaa cttccctcga caccggcgaa aaaccagtat
     1921 aaatcgagca cctgctggag tttttcatcg gtttcttttt tagaatcaga actgctccgt
     1981 ttggggaaag gtgaatacga cctgtcggag atgtttgtgg tgcacaatac ctaccaggca
     2041 aaggcaaaag gctacgcccg ctggcagggg aacgtcaatt ttgctgccgg tggcgctttt
     2101 cacgatgttc ttgcagtaat ggaaactgcc gggatgattc ctgaggaagt ttacagcggt
     2161 aaagtcatcg gggagaagga acatattcat aatgagatgg atgcggtgct taccgggttc
     2221 atggaagcac tgatcaagaa tgataacggg aagctcacac cggtatggcc attggcggtg
     2281 aagggcatcc tggatgccta cctgggcgaa tatccttcgt cattcaaata caaaggcaaa
     2341 gaatacactc cgaaatcttt tgccgaaagc ctgggccttg acctgagcca gtatgtggaa
     2401 atcggctcat actcccatca tcctttttat gaaaaattta ttattgaatt gccggataac
     2461 tggatgatgg atgaaattta caatgttccc ctggatgaga tgatggagat cattgattat
     2521 tcactggata acgggtttac ggtcggatgg ggcgccgata tcagcgaaaa gggtttctcc
     2581 tggacaaatg gcgtagcagt cgttccggta gttgacctgg aggatatgac cggaaccgaa
     2641 agggaaaaat gggagaagct gacccagaaa gagcgtgaaa aaatgatcta cagctttgag
     2701 aagccggtcc cggagcgggt cattacccag gaactgcgac agacagcctt cgataactac
     2761 cagaccacgg atgaccatgg gatgcacatt accggaacgg cgaaagatca gaacggccag
     2821 ccctattatt atgtcaagaa ctcctgggga gttacaggta atgattacaa aggattcttc
     2881 tttgcttcaa aaccttttgt ggcctacaaa accatcgata tcctggttca caaggatggc
     2941 atcccgaagg aaataagaaa aaagctgggg ctgttgtaaa tcagggaagg atacggtcat
     3001 ccggtgtgga acggcctatt tcgcagtatt gagaacttcc gggccaccgg tgccggttcc
     3061 gggcgatgag gtcgtaaatg gtatcgcgcc aggataccgg gatcacagcg aatattcctg
     3121 caaccgacca gggaaatccg agcagtatgg ctattctgat ggctgcctga gatttcatga
     3181 ggattttgcc ttcctcatag aggatgacgg agtcatcttt tagattaatt atttccaatg
     3241 ttttagccgt caccgattgc aacgatgcaa accggaatct tttcttccgg tcatatttca
     3301 ggataaatcg caccgaacgg gcacagaggt ggcaggagcc gtcataaaat acggtcggaa
     3361 agccggaacc tcctgatttt tttgttgttt tcatcttatt tgtaatgaac aaaaaataag
     3421 atagatggtt ttcaagatat taaaaacttt gtatttttac cgacctatcc ccaaattcac
     3481 agattattag gagaacctgt attatgactg gagagaagat tacaaaaagc ggcggaagat
     3541 taaatgtacc cgatattccc gtaataccgt tcattcgtgg cgatggtacc ggccctgata
     3601 tctgggcggc gtcggtgcgt gttttcgatg cggctgttga gaaagcatat catggaaaga
     3661 agagagtgga atggctcgag gtttatgccg gtgaagaagc ttttaaccgg tttggagagt
     3721 ggctgccaca ggcaacgatc gatactttca gggattacct ggtagggata aagggtccac
     3781 tgacaacgcc ggtaggaggg gggatccgct cgcttaacgt cgcactcagg caattactgg
     3841 atctttatgt gtgtttgcgc ccggtaaaat atgtcaccgg ggtaccttct cccgtaaaac
     3901 atcccgagcg ggtcgatatg gtcattttcc gggagaatac ggaagatatt tatgcgggga
     3961 tcgaatggaa aagcggatcg gaagaggtga aaaaagtgat ccgtttcctg gaagaagaga
     4021 tgaaagtgaa gaagatccgc ttcccggaaa catcagggat cggaataaaa ccggtttcgg
     4081 aagagggttc caaacgcctt atccggtcgg ccatccgcta tgcccttgag gagaatcgcc
     4141 attcggtcac gctggtgcat aaaggcaata tcatgaaatt cactgaaggc gctttcaaga
     4201 cgtgggggta tgaagtggca acgcaggagt tccgcgacca tgtggtgaca cagcgcgaaa
     4261 gctggattct gggaaatatc gacaaggatc ctacgatttc tggagaaaat aatgcgcgcc
     4321 tgatcgaacc gggttattat atgatgacac cggaacagca gcaggctatt aaagacgaag
     4381 tgggggaagg gatgaagctg ttgccgaccc atgggaacgg gcagtggaag acgaagctga
     4441 tgataaagga ttcgatagcc gatattacgc tgcagcaggt actgacccgt gccgatgaat
     4501 tcgaggtggt cgccacgccg aacctgaacg gcgattacct gtcggatgcc ctggcggccc
     4561 aggttggcgg catcgggatt gcacccggcg ccaatataaa ctacgatacc ggacatgcca
     4621 tttttgaggc aacccacggc acagcgccca aatatgccgg tctcgaccag gtgaaccccg
     4681 gttcggtgat cctctccggc gcaatgatgt ttaaatacct cggctggcat gaagtgcacg
     4741 acctgatctg gaaaggcctg gaaaaagccg tttcgggtaa aagagttacc tacgattttc
     4801 accgccagat ggaaggggct gttctgctca agtgctccga atttggggaa gagattgtgg
     4861 agaatatgaa ataggaatgt aggacttaaa gacaataggt caatagaaca atagaacaat
     4921 agaacaatag aacaatagaa caatagaaca atagannnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnntagaa caatagaaca atagaacaat agaacaatag aacaatagaa
     5101 caatagaaca tttaaccctt tatcgcaaat gatcggactg aaagagaaat tacggcaaaa
     5161 gatagaaggc tggaggccaa tgacaaagcg cctggtgcag gaattcggag atgttgtgat
     5221 cgacgaggtc accatcgaga aagtcattgg cgggatgagg gacctgaagg tgctgataac
     5281 ggatatatcc tacctggacc cgaatgaagg catccgttac aggagctata cgttaaatga
     5341 agtgattgag aagcttccca agccaaaagg cgcggaaatg ccctatgttg aaggagtatg
     5401 gtatctcctg ctaacgggtg atatcccgac agaggaagaa gtacaggagg tcgtggagga
     5461 gttccagaag aggagaatcc tgcccagggt catctacgaa gtgatcgatg cggccccttg
     5521 ctgcaaccac ccgatgacga cctttgcctc ggctatcctg gcactgcacc gtgaatcttt
     5581 cttcgtaaaa aaatacaatg ccggcatacc aaaaagcgaa tactgggacc cgatgtacga
     5641 agatgcgatg aacctgctgg ccaagctctg cgaaatagcc acttacatct ataccaagct
     5701 gtataaagac ggcaagcgga tccagtcgaa ccccaacctc gacatgggcg ccaattttgc
     5761 ctggatgatg ggtatcaacc cgccatacga tgatgtatcc cggatgcatt ttatcctgca
     5821 tgccgatcac gaaagcggga atgtcagtgc gcataccggc cacctggtag ccagttcgct
     5881 gtcggatgtc tatctgtcca tcgccgctat gatcaacggg ctggcaggtc ctctgcacgg
     5941 cctggccaac caggaagtgc ttcgctggct gcaagacctg cgggagcaac tcggaggtga
     6001 cgatccgacg gaagaggata tccggaaata tgtttgggat acgctgcaca acggacatgt
     6061 tatcccgggc tacggccacg ccgttttgcg caagaccgac ccgaggtaca ccattcagcg
     6121 tgaattcagc ctgaaacaca tgccggaaga tccgttgttc aaatataccg acctgctcta
     6181 taaagtggtg cctgagatcc ttattcagca aggaaaagca aagaatccat ggccgaacgt
     6241 cgacgcgcag tcgggaatta tccagtggca ttacggggtg cggcagtatg atttctatac
     6301 cgtcctgttt ggcatcgggc gggctattgg tatttgcgcc aatatcatct gggaccgtgc
     6361 attaatgtac ccgctggagc gccccaaatc gataacaacg gaaatgctgg aagaaatcgt
     6421 cggaataaaa caaaaggaat agcctctccc ggtacttttt cccttttcct tattccttgt
     6481 tcccttttgt cttttccctt cccggcagtt caacatggaa ccttgtgccg gatccgggtt
     6541 cgctttcgca tctgacagtt ccgtgcatgg ccgttgcgta tttatatact atggccagcc
     6601 ccagtccgtt gccggccgat tcctggtcaa acatcgatgt ctggcgttta tagcggtcaa
     6661 agactttctc catcttttct ttttccattc cggggccttc gtcgcaaaca caaaacatga
     6721 tcttatcttc attttccccg agtctcactt caatcgtttt cccgtcgggc gtaaatttga
     6781 tggcattcga aagcaggttg tcgaatatct gcgtcacgta aacccggttc agccgggctg
     6841 tgatatcgac catttgcacc ttaaagttaa tgtgcttctg gtcaataaaa taagaatagt
     6901 tccggatcag atcttcgagg atctccctga cattcaatgg ttccatcttc aattcgtaaa
     6961 ctttcgattc gatggcattg atatcaagga tctcattgat aagattattg atccttttta
     7021 tgctgttccg gatgatggcc agcccttcac gttcggtttc atccacaaaa ccgtccctgg
     7081 cgtccagcat atcggccata cacaggctgc tggtcagcgg attttttatt tgatggataa
     7141 cagtgccgat caactggttt ttttcttcat tcaatttggt cagatcgaaa ttttgctgtt
     7201 caagcatcgc tttttgttgc atgatgctgg cattctgttt ttcgataagc tctttctgac
     7261 gctgtatctt gaggtagctt cgtgaaagct tctcttccag ctcgttcata tccctcatcc
     7321 ggttgatcag ttccttgagc acacctctta aaatgccggg atccccggcc gcaatacgat
     7381 aaaagtcgcg gttatcgagc cgtagaagcc ggcaattgtc ttcggctgtc accgatgccg
     7441 accgctgccg ctgatcgatg agagaatact ctccgaacat ttgcccctgc tccagcctgg
     7501 ccagaacatg atttccgtcg tgaaccctga ccctgccgcc ggctatgata aataccgcgt
     7561 ccgcttcatc gcctttgcga ataacgatac tccctttcag tgcctcattt tctgacatgg
     7621 aatcggccag ttcgagcagg accttattat ctgctccgct gaacatgggc aaattccgga
     7681 tgaattcgaa tttttgttcc tgggtaagtg atgccatccg tctgttgatt tattgataga
     7741 cgatcataga ccgatcaggt tacttctgaa gcgatttata tgatcggtaa tttcggggaa
     7801 aaaacctctg aactcagctt catagagcga atagccggcc accaggtcac ctatggcatt
     7861 ttccatcccc gacggcttgt ttatccgtcg cgacatcccg ttaaaaaccc gttgcatggt
     7921 atccaggtcc cggtaattga ccagccagtt ctgggtgatc ataaacggca gtatccgcct
     7981 ggatcttgcc ggtaacatat gaaatttgcg gatcagcaga aaataggtgt tcgcaaccag
     8041 gcctctcaag tcttaattcg agt
//
LOCUS       DAOQXS010000058         7992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9503_length_7992_cov_4.221746, whole genome shotgun sequence.
ACCESSION   DAOQXS010000058 DAOQXS010000000
VERSION     DAOQXS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9503_length_7992_cov_4.221746"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..603
                     /locus_tag="PKM34_02815"
     CDS             142..603
                     /locus_tag="PKM34_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82545.1"
                     /translation="MYKLLINSIFAMLMASVVFGQAQQGADKLYYDKDGRLFTGITRD
                     YYPDSALHAEFEIQNGVLSGISKVYFNNGKLEEIRSFREGMMHGKWEKWNQQEVKIAE
                     ANYTDNLKDGKWYIWDENGTLRYDMTYIKGEKSGVWMMYDEKGKVISQKSY"
     gene            712..1059
                     /locus_tag="PKM34_02820"
     CDS             712..1059
                     /locus_tag="PKM34_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ82546.1"
                     /translation="MKKAINIFAIVVLSSFGIAAFADNNPDLTPKSVKITGQVVDKVN
                     QEVLAGALVKVEGTDIEVYTDFDGNFSIEGIAPDKYTLKCSLISYTECEKEICIDTAG
                     ETVEIELENFTSK"
     gene            complement(1113..2165)
                     /locus_tag="PKM34_02825"
     CDS             complement(1113..2165)
                     /locus_tag="PKM34_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82547.1"
                     /translation="MIRFTNPNRLALLISAANSLFISMVYGVGIMGNMIPFSWPFFFI
                     INALLFLFSYFFIRYIIQNFIFQKIKVIYKIIRSLKLSRTEKLEWRSNLGEDMIGQVS
                     QDVMDWAENKTAEIEQLRNIENYRREFLANVSHELKTPISNMQGYISTLISGGIDDPE
                     INRDYLQKTEKNINRLIELVDDLDIISSLESGEVNMDKSKFDMRALTREVFEFLEDSA
                     KSRGISFLFGNDDNNPVFVYADRYRIRQVLVNLVDNSIKYGRDGGRTKVSFYDMDDNY
                     LIEVSDNGIGIEEQHIPRLFERFYRVDKHRSRTEGGTGLGLAIVKHILEAHDQTVNVR
                     SSPKIGSTFSFTLRKG"
     gene            complement(2174..2851)
                     /gene="phoU"
                     /locus_tag="PKM34_02830"
     CDS             complement(2174..2851)
                     /gene="phoU"
                     /locus_tag="PKM34_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNQ82548.1"
                     /translation="MNHLEVELKNLKSSLLEMWSIVISQHEKAKMALDNHDKELANEI
                     HTNEKLLDAFELKHDMDCENLLVLNNPVAVDLRFVLAVLKINYNLERIGDYANYIANT
                     IRKSETPFDNKLLKDLNIGQMFDTAHLMMVGAYEAFDKCETSHVRHFSELDNKLDQWN
                     KDSFRILSEYILKYPTESKQALELFSIIRKLERVGDHNQNIAEEIVFYLDARVIKHKK
                     LHHKPNN"
     gene            complement(2890..3642)
                     /gene="pstB"
                     /locus_tag="PKM34_02835"
     CDS             complement(2890..3642)
                     /gene="pstB"
                     /locus_tag="PKM34_02835"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNQ82549.1"
                     /translation="MYKAEAKNVNFYYGDYLALKDINLSFPDKTVTALIGPSGCGKST
                     FLRLFNRMNDLIDHVKLEGEVLVNGENIYSKDIVIDELRKSVGMVFQKPNPFPKSVFE
                     NVAYGLRVNGVRDKNYIEDVVVNSLKKAALWDEVKDKLKKSAYELSGGQQQRLCIARA
                     LAVEPTLILMDEPASALDPISTAKIEELIHELKQQYTIIIVTHNLQQAGRVSDFTSFF
                     YLGELVEHDVTKKMFLNPGDERTQNYITGRFG"
     gene            complement(3654..4520)
                     /gene="pstA"
                     /locus_tag="PKM34_02840"
     CDS             complement(3654..4520)
                     /gene="pstA"
                     /locus_tag="PKM34_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNQ82550.1"
                     /translation="MMAMTKKRIIQKIAFSAFGAVSFIVVAVLFIILGFIAFKGAGVI
                     SWEFLTAMPEQGMTQGGILPAIIGTACLMVGSMIFAFPVGVLSGIYINEYLKDSFFKR
                     FVRMMTDNLAGIPSIVFGLFGMALFVNYLDFGDSIIAGSLTLGLLVLPVVIRTTEEAL
                     KSVDDSLRVGSLALGATKLQTIQRGVLPVAFPNIITGLILSIGRVSGETAPILFTVAA
                     YFLPRLPNSIFDQVMALPYHLYVLTTSGTNIEESRPMAYGTALVLILIVLLINVMAST
                     LRKYLGKKVKMN"
     gene            complement(4520..5725)
                     /gene="pstC"
                     /locus_tag="PKM34_02845"
     CDS             complement(4520..5725)
                     /gene="pstC"
                     /locus_tag="PKM34_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNQ82551.1"
                     /translation="MRKYRKIWEKLAESVLFSSSTVTSLTVILIIVFLWREGVGLFNS
                     KPVDKGYVFALNQANPVNTLSAKQLKDIFNQDITNWKEVGGANDTIILLTINEIPNYF
                     TEEEMGTDLENLPVLIDRLILENSGAIVFISENYMPQDFHGRILDVEKISLKEFTFGT
                     EWYPTAEPVPLFGVWPLILGTLLVTLGAIVFALPLGLATAIYLAEVSSFRMRNIIKPL
                     VELLAGIPSVVFGFFGLVVLVPLIQQVFSLDVGETALAGSIILGIMALPTIITISEDA
                     LRTTPKSLKEASLALGATRWQTMVRVTIPYARSGITTAIILGIGRAIGETMAVLMVTG
                     NAAMIPTSFLMPVRTIPATIAAELGESPYGGLHFQALFALGIILFLFTLIINFFIELV
                     KGRKHIKTL"
     gene            complement(5750..6544)
                     /locus_tag="PKM34_02850"
     CDS             complement(5750..6544)
                     /locus_tag="PKM34_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ82552.1"
                     /translation="MALLTQVFPQNVSIKVKGSDTVLPLTQKEAEVFMKKFPGSSVMV
                     TGGGSGVGLAALQNGTADIAQSSRKMKLDEKMKMQDAGKAFTEVIIANDALAVIVHPS
                     NPVTQMTREQIEGIYTGKITNWKDVGGEDLKIVMYSRETSSGTYEFFKEHVMNKKNFA
                     PTAMLMPATGAIVQSVSQTKGAIGYVGLAYLESDIRPVKVSYDKGQTYVSPTVENAVN
                     KTYPISRPLYYYYLNNVSASVKPFIDFILSAEGQKIVKEVGYVPVK"
     gene            complement(6643..>7992)
                     /locus_tag="PKM34_02855"
     CDS             complement(6643..>7992)
                     /locus_tag="PKM34_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNQ82553.1"
                     /translation="MVITVNHKLTISLFLIWIFALPFVSYSQVNPENDVNLAVDTLNA
                     KVETVKSDMDLAKKLKISGYVQAQWQLADTAGQSSSFSGGNFPKNSDNRFQLRRGRIK
                     FGYKGKLTQFVFQIDATQSGVALRDAYMNFTDPWLQMFTVTGGLFKRPFGYEVLQSSG
                     DRETPERSRIAQSLFPGERDLGAMITIQPRKESRFNFIRLSAALISGNAIAPEIDSRK
                     DFVGQLGINRTTRNEAFRYAMNVSYYNGGYFQETKKVYTLESLANGIYGYIVDSTETN
                     KGEYAKRQYFGADAQFVYDWKPGTTTIRGEYIFGDQPGSAKSNLSPTNRTFSSTDPLD
                     TYNRHFSGGYVYFIQNILHSRHEIVIKYDWFDPNTKVAGNEIVSAVTVEEASVKTNLS
                     AADIMYSTWGFGYSVRIASNVKLTAYYEMVKNETTQLAGFAKDVKDNVLTIRAQFKF"
BASE COUNT         2083 a         1827 c         1835 g         2247 t
ORIGIN      
        1 agtatttttc aaggctttcg caggggatta cttttcagat acggttcagt tattcgtttt
       61 tctgatcttt tcaggacaga atattgcggc atcatttttt cttaatcatt aaaatcttaa
      121 attcgtttaa atatctaaaa catgtataaa ctcttgatca attctatttt cgctatgctg
      181 atggcctcag tagttttcgg acaggctcag cagggtgccg acaagcttta ttatgataag
      241 gatggcaggc ttttcacagg cattacccgc gattattatc ctgattcagc ccttcatgcc
      301 gaatttgaaa tccaaaacgg cgtattaagc ggtatcagca aggtttattt taacaatgga
      361 aaactggagg agatcaggtc gttcagagag ggtatgatgc acggcaaatg ggaaaagtgg
      421 aatcagcagg aggtcaaaat tgccgaagcc aattacacgg ataacctcaa ggatggaaaa
      481 tggtatatct gggacgagaa tgggacatta agatatgata tgacctatat caaaggagaa
      541 aaatcgggtg tttggatgat gtacgatgag aagggaaagg tcatctcaca aaaatcctat
      601 taacaaaaca gtttataatc cccctcaaac tttacatata cctaaaactg gcttaatgat
      661 ttcttaacat tgaggattta cttttgcgga gtaaaatcaa gaatcacagc catgaaaaaa
      721 gccattaata ttttcgcgat cgttgtttta tcatcctttg ggattgccgc ctttgctgac
      781 aataatcccg atctcacccc caaatcggtt aaaatcacag gacaggttgt agacaaagta
      841 aaccaggaag tgctggccgg ggccctggtt aaggttgagg ggactgacat tgaggtttac
      901 accgattttg acggaaattt ttcaattgaa ggtattgcgc ctgacaaata cactttaaaa
      961 tgctccctga tctcttatac ggaatgcgag aaggaaattt gtattgatac tgccggagag
     1021 accgtggaaa tagagcttga aaatttcact tccaaatagt ccgcgcgtta ataatagaaa
     1081 agagggtgtc gctcatcggc accctctttt ttttatccct tcctgagtgt aaatgaaaaa
     1141 gtagagccga tcttcgggga acttctgacg ttaaccgttt gatcgtgggc ttcgagaatg
     1201 tgtttaacga tagccaggcc cagtccggtg cctccttccg tgcgtgaacg atgcttatcg
     1261 accctgtaaa aacgctcaaa caacctgggt atatgctgtt cttcgatccc aattccattg
     1321 tcggaaacct ctatcaggta attatcgtcc atatcataaa aactgacctt ggtgcggccc
     1381 ccgtcacgcc cgtattttat ggaattatcg accagattga ccaatacctg cctgatcctg
     1441 tagcggtcgg catagacgaa aaccgggttg ttgtcatcat ttccgaataa gaaacttata
     1501 cctctgcttt ttgcgctgtc ttcgaggaat tcaaagactt cgcgggttaa agccctcata
     1561 tcgaactttg atttatccat gttgacttca cctgattcca ggctggaaat tatatcgagg
     1621 tcatcgacca gttcgataag acggttgata tttttttctg ttttctgcag gtaatccctg
     1681 ttgatctcgg gatcgtcgat accgcctgag atgagggtgg agatatagcc ctgcatattc
     1741 gaaataggcg ttttcagttc atgcgataca ttggccagga actcccgcct gtagttctct
     1801 atattcctaa gctgttcgat ttcagcagtt ttgttctcag cccagtccat cacatcctgg
     1861 cttacctgcc cgatcatgtc ctccccaaga tttgaccgcc attccagttt ttccgtgcgc
     1921 gacaatttca gtgaacggat gattttataa atgactttga tcttctgaaa aatgaaattc
     1981 tggataatat aacggataaa gaaatatgaa aatagaaaga gcaatgcatt gataataaaa
     2041 aagaaaggcc agctgaaagg gatcatattc cccataatcc cgaccccata taccatgctg
     2101 ataaataaag aattagcagc agagataagc aatgcaagcc ggttaggatt ggtgaacctg
     2161 atcattgcag tgttcaatta ttgggcttgt ggtgtaattt tttatgtttg atgaccctgg
     2221 cgtcgaggta aaaaacgatt tcctcggcaa tattctggtt atggtcgccg actctttcca
     2281 gtttccggat gatagagaag agctccaggg cttgttttga ttccgtagga tatttgagta
     2341 tatattcgct caatatcctg aacgaatcct tgttccattg atccagttta ttgtccaact
     2401 ctgaaaaatg cctcacgtgg cttgtttcac atttatcgaa ggcttcatag gctccgacca
     2461 tcatcaggtg agccgtatcg aacatctgcc cgatgttcaa atccttaaga agcttattgt
     2521 cgaaaggggt ttcagatttg cgaatggtat tggcgatata attggcgtaa tccccaatgc
     2581 gttccaggtt gtaattgatt ttaagtacgg ccaggacgaa ccggaggtcg acggcgaccg
     2641 ggttattcag caccaggagg ttctcgcagt ccatatcatg tttcaactca aaggcgtcga
     2701 gcagtttttc attggtatgg atctcattgg cgagttcctt atcatgatta tccagggcca
     2761 tctttgcctt ctcgtgctgg ctgatcacaa tcgaccacat ctccagcagg ctcgatttca
     2821 ggttttttaa ttcgacttcc aggtgattca tctgaataaa ttttaagata attgatagag
     2881 ttacaatcat taaccgaacc tgcccgtgat atagttctgg gttctttcat cgccgggatt
     2941 taaaaacatc ttcttggtta catcatgttc aaccagttcc cccaggtaaa agaacgacgt
     3001 aaaatcgctc acgcggcctg cttgttgtaa attatgtgta acaatgatga tcgtatattg
     3061 ttgcttcagt tcatggatca gttcttcgat tttggctgtc gaaatcggat caagagcgga
     3121 ggcaggttca tccatcagga tgagggtggg ttcgacggcc agggcgcggg cgatgcaaag
     3181 ccgttgctgc tggcctcctg acagttcata ggccgatttc ttcagtttat cctttacctc
     3241 atcccataac gccgcctttt tcaatgagtt caccacaaca tcttcgatgt agttcttatc
     3301 gcgtaccccg ttcacccgta atccgtacgc cacattctcg aataccgatt tcgggaacgg
     3361 attcggcttc tggaatacca tcccgacact tttccgcagc tcatcgatca cgatatcttt
     3421 cgaataaata ttttctccat tcaccaggac ttcgccttcg agttttacat gatcgatcag
     3481 gtcgttcatc cggttaaaga gccggagaaa cgtcgatttt ccgcatccgg acggaccgat
     3541 gagcgctgtt acggttttat caggaaaaga gaggttaata tccttcaaag cgagataatc
     3601 gccgtaataa aagttcacat tctttgcttc cgctttatac atggtcaata atcctaattc
     3661 attttaactt ttttgccgag gtatttgcgc agggtgctgg ccatgacatt gatcaataac
     3721 acgatcaata tcaataccag tgcggtgcca tacgccattg gccgtgattc ttcgatatta
     3781 gttccgctgg tggtgagcac atacaggtgg tagggcaggg ccatcacctg gtcgaagatg
     3841 ctgtttggaa ggcggggcag gaaatacgct gccacagtaa acaggatagg ggccgtttca
     3901 cccgataccc gcccgataga gaggatcagg ccggtgatga tgttcggaaa tgccaccgga
     3961 aggacgcctc tctggatggt ttgcagcttg gtggcaccaa gagcaagact accgaccctg
     4021 agagaatcgt cgaccgactt cagggcttct tccgtcgttc ggatgacaac gggcagcacc
     4081 agtaaaccca gcgtaagcga acccgctatg attgaatctc caaaatcgag ataattaacg
     4141 aacaatgcca taccaaatag tccaaagaca atagaaggga ttccggccag gttatcggtc
     4201 atcatcctga caaaacgctt gaaaaagctg tctttcagat attcattgat atagatcccg
     4261 gagagtacgc cgaccggaaa ggcgaagatc atacttccga ccatcaggca ggccgttccg
     4321 atgatagccg gaaggattcc cccctgggtc attccctgtt cgggcattgc agtcaggaat
     4381 tcccagctta tgacgccggc tcctttgaaa gcaataaaac ccaggattat aaacagcacg
     4441 gccacaacaa tgaaactgac cgctccgaat gctgaaaaag ctattttctg tatgatcctt
     4501 ttttttgtca tcgccatcat cagagcgttt taatatgttt acggccttta accagttcga
     4561 tgaagaagtt tataatgaga gtaaagagga agaggatgat gcccaaagcg aagagcgcct
     4621 ggaaatgaag cccgccatac ggggattctc ctaactcggc ggcaatggta gcggggatgg
     4681 tacggaccgg catcaggaat gatgttggga tcatggctgc atttcctgtg accatcagga
     4741 cggccatggt ttcgccgatg gcccgcccaa taccgagaat gatggctgtt gtaatccctg
     4801 agcgggcata ggggatggtt acacgcacca tagtttgcca gcgtgtggcg ccaagagcca
     4861 ggctggcctc tttcagcgat tttggcgtgg ttcgcagggc gtcctctgaa atagtgatga
     4921 tggtaggcaa agccataatc cccaggatga tactgccggc cagggcggtt tcacctacat
     4981 ccaggctgaa aacctgctgg atcagaggta ccaggaccac cagcccgaaa aatccgaaga
     5041 cgaccgatgg gataccggca agcagctcga ccagtggttt gataatattc ctcatccgga
     5101 aagaagaaac ctcggccagg taaatggccg ttgccaggcc taatggaagg gcaaaaacga
     5161 tggccccgag cgtcacgagc aaggtcccca ggatcaatgg ccacaccccg aacagaggca
     5221 ccggctctgc tgtcggatac cactctgtcc caaaagtgaa ctccttaaga ctgatttttt
     5281 ccacatccag gatccggcca tggaaatcct ggggcatata gttctcagat atgaatacaa
     5341 ttgcaccgga attttccagg atgagcctgt cgatcagcac cggaagattt tccagatcgg
     5401 tgcccatctc ctcttccgtg aaatagtttg gtatttcgtt aattgtcagg aggatgatgg
     5461 tgtcgttagc tccgccgacc tctttccagt ttgtaatatc ctggttaaag atatctttga
     5521 gttgtttggc gctcaaggtg ttaaccggat tcgcctgatt cagggcaaaa acatatcctt
     5581 tatcgacagg cttgctgttg aataatccta caccttctct ccagagaaaa acaatgatca
     5641 ggatgacggt caggctggtg actgtgctgc ttgaaaagag cacgctttca gccagttttt
     5701 cccatatttt ccggtacttt ctcaacccag taatttatta tcaaatatac tatttcacag
     5761 gaacataacc cacttcctta acaattttct gcccttcagc cgacaggata aagtcgatga
     5821 acggcttaac cgacgctgac acattgttca ggtaataata gtagagcgga cgcgagatag
     5881 ggtatgtctt attcacagca ttttcaactg tgggtgacac ataggtttgt cctttatcgt
     5941 agctgacctt aaccggcctg atgtcggatt ccaggtaggc caggcccacg taaccgattg
     6001 cgcctttggt ctggctgacc gactgtacga tggcccccgt ggccggcatc agcatggccg
     6061 tcggcgcgaa atttttcttg ttcatgacat gttccttaaa gaactcatag gtacccgaac
     6121 tggtctccct ggaatacata acgattttca gatcttcacc tccaacatct ttccagttgg
     6181 ttattttacc ggtgtagatc ccttcgattt gctcgcgggt catttgggtg accgggtttg
     6241 aagggtgcac aatgacggcc agcgcatcgt tggcaatgat cacctcggta aaggccttgc
     6301 cggcgtcctg catcttcatt ttttcatcca gtttcatttt acgcgacgac tgcgcgatat
     6361 cggctgtgcc attctgaagc gctgccaggc ctacacctga accacccccg gtaaccatca
     6421 ccgatgatcc gggaaacttt ttcataaata cttctgcttc tttctgtgta aggggtagta
     6481 ccgtgtcgct cccttttacc ttgatgctga cattctgagg gaacacctgc gtaagaaggg
     6541 ccatgaatac gattgctaat gttatttttt tcatttttat tattttattt aacagtttat
     6601 aaaccaatac tttaatcagg aacttatatt aagaatttac aattaaaatt taaattgtgc
     6661 tctgattgtc agcacattgt ctttcacatc tttagcaaag cctgccagtt gggttgtttc
     6721 atttttaacc atttcataat aagcggttag tttgacattg gacgcgatac ggacactata
     6781 gccgaatccc caggtggagt acatgatgtc ggcggcgctc aaattagttt taacactggc
     6841 ttcttcgacc gtcaccgcag acacgatttc attgcccgct actttggtat tgggatcgaa
     6901 ccaatcgtat ttgatcacga tttcgtgcct ggagtggaga atattctgga taaaatatac
     6961 gtatccacca ctgaaatgtc tgttatatgt gtcaagaggg tcggtggagg aaaacgtccg
     7021 gtttgtaggg ctgaggttag atttggcaga accaggctga tcgccgaaga tgtattcgcc
     7081 ccggatggtg gtcgtccccg gtttccagtc atagacgaac tgggcatctg ccccgaagta
     7141 ttgcctttta gcatactccc ctttgtttgt ttccgtcgaa tccacaatat atccgtaaat
     7201 tccgtttgcc agcgattcaa gggtataaac cttctttgtc tcctgaaaat agccaccatt
     7261 atagtaggat acattcattg catacctgaa cgcttcgttg cgggttgtgc ggttaatgcc
     7321 gagttgaccg acgaagtctt tccgggaatc gatttccggc gcaatagcat ttccgctgat
     7381 taaagctgcg ctcaggcgga tgaaattgaa cctcgactct ttacgtggct gaattgttat
     7441 catggcgcca aggtcacgct cgccggggaa gagcgactga gcgatcctcg atctttcggg
     7501 agtctcccgg tcaccggatg attggaggac ctcataaccg aacggacgtt tgaagagccc
     7561 gccggtaacg gtaaacattt gcaaccaggg atcggtaaaa ttcatatagg catctctcag
     7621 tgcgacaccg ctttgtgtgg cgtcaatctg aaatacaaat tgtgtcagtt tacctttata
     7681 accgaattta atgcggcctc ttcttaattg gaaccggtta tccgaatttt tcgggaaatt
     7741 tcctcctgaa aaggatgaac tttgacctgc cgtgtcggcc aattgccatt gagcctggac
     7801 gtatccggag attttcagtt tttttgccag gtccatatca gacttaactg tctcaacttt
     7861 tgcgttgagc gtatcgactg cgagattcac atcattttca ggatttacct gtgaatagga
     7921 tacaaacgga agcgcaaaga tccagatcag aaataaactg attgttaatt tgtggtttac
     7981 tgttattacc at
//
LOCUS       DAOQXS010000059         7940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9582_length_7940_cov_3.796449, whole genome shotgun sequence.
ACCESSION   DAOQXS010000059 DAOQXS010000000
VERSION     DAOQXS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_7940_cov_3.796449"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKM34_02860"
     CDS             <1..315
                     /locus_tag="PKM34_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ82554.1"
                     /translation="QVMTSLFGNQLNMDEANKTIVFERQGLIFVFNFHPTHSIADYAF
                     PVPRHGEYRIILNSDSKAFGGHGRIDDSITYYTFSDPSGNWPYLKIYNTNRTALVLKF
                     DL"
     gene            complement(341..1633)
                     /locus_tag="PKM34_02865"
     CDS             complement(341..1633)
                     /locus_tag="PKM34_02865"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNQ82555.1"
                     /translation="MKNFLLTLALVLFSLSVYSQWYQHTDIPVSTNLSGITFIDSFHG
                     WILGDSGTILHTNDGGSTWEIQYCPVGVSFGDISMIDSLNGWIVGHGPDTSGYILKTT
                     DGGTNWILQDVSLHNLQDVCFTDSLHGWMIGESGERLPCMLRSTFDGGATWSEAIINE
                     VQIPYDVIFTDSMTGFIGGTYHNGSDLCARIIKTTDGGATWQVKLNWCSDPFCFYNDM
                     AFANDSVGWLVGESWIEGIHGAEADFGFIMATQDGGETWHEIFSDEIFPVEACLLSDQ
                     SGWAVGGDGFFMYTGNGGNTWDMMNLPPEESLYDIFFIDDDTGWAVGTYGTVMYTNNN
                     GTMEVPEVPDDPTVLKAVPNPAHGELTMNFTVDKGGPATVELVDLSGRTVVQMPGKML
                     FDGPNRLTLQWGNEVTPGIYFLRIVSASGILTGKILVE"
     gene            1760..2002
                     /locus_tag="PKM34_02870"
     CDS             1760..2002
                     /locus_tag="PKM34_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNQ82556.1"
                     /translation="MKPITFTSTLPAEIWQSLEAYAEKFKVPRNRIMEQALKAYFERL
                     KQAEYIHSFRKAAGDPEMGAMAAEGLADYLKILDEP"
     gene            1999..2331
                     /locus_tag="PKM34_02875"
     CDS             1999..2331
                     /locus_tag="PKM34_02875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ82557.1"
                     /translation="MKQREIWMADLNPVKGSEQQGIQPVVVISGNAMNDNLGIGIICP
                     LTSKIKHYAGCLVFKKDDINRLDDDSEIITFQVRTIAKERLISKTGEITKSQLEAIKK
                     GLQEILTY"
     gene            2705..3832
                     /locus_tag="PKM34_02880"
     CDS             2705..3832
                     /locus_tag="PKM34_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ82558.1"
                     /translation="MDTPFIFGKLATGENFTDREAEQQRLIQNFRSGTNTILISPRRW
                     GKSSLVHKSAQEAKKAEHAPIMVFIDLFNIRSEEDFYKCLAESVIRATSGRMEEIMSN
                     IKEFMKEWVPRISFSPDAHHEFSLSLDWAEIKKQPDEILNLAENIAVAKNRKIVICLD
                     EFQNIAFYENPLAFQKKLRSHWQKHQMASYCIYGSKRHMLLDVFTSPSMPFYRFGDMM
                     FLPKISIDYWIPFIQERFKSTGKDIDREQAERIATLTECHPYYVQQLAQQTWLRTEKA
                     VTIGIIDEALDNLVLQMSLLFQNITEDLTTSYVNFLKMILDGHTRYTSMENIDRYQLG
                     TSANVIRIRKALISKEIIDEYEGKAILLDPLYAIWLKKYYF"
     gene            complement(3854..4333)
                     /locus_tag="PKM34_02885"
     CDS             complement(3854..4333)
                     /locus_tag="PKM34_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82559.1"
                     /translation="MIDIYDSYTDTWSVDHMTNPLVMHACAGAEDHFLVAGGLVYEDP
                     DSLYLTSKVEIFTDPDCSVGVGPEQGDGGLQPAVSVYPNPTHGIVELQFTVYNLQSAT
                     LKIYNAHGQEVASLLDGMCYGDQVIRWDMSRLPAGIYYYRTSNIEHRQSTMGKIVKY"
     gene            4499..5746
                     /locus_tag="PKM34_02890"
     CDS             4499..5746
                     /locus_tag="PKM34_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82560.1"
                     /translation="MRDLLKTVIADQLTLTWSESFTSRNVPETLLKGEDIVVISGIRR
                     CGKSTLLHQIRSGYSEKDYYMNFDDERLLNFKVENFQLLHETFIELFGQQKTFWFDEI
                     QNVKGWERFVRRLHDYGNKVFITGSNATMLSRDLGTHLTGRFLKHELFPFSFREFLDF
                     NNIKTGIDNIYSTGEKAGIKRLFNEYFKTGGFPYYLKYEDDNYLKSLYESILYRDIMA
                     KHNLTSERELKELIYLLASNVSRPYSNNRLAASIGLKNATTVKNYLDYIQDTYLLFAV
                     NKFDYSARKQLHNARKIYFIDNALIRKLGFMFSEDKGRMLENLVFIELQRRGEEVFYY
                     KGKGECDFVLRDGNSITGAVQVTYSMTANETRDREIKGLLEAMDSYNLSEGIILTDDT
                     EENLSVNGNRILIMPVYKWLLRI"
     gene            complement(5797..6261)
                     /locus_tag="PKM34_02895"
     CDS             complement(5797..6261)
                     /locus_tag="PKM34_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ82561.1"
                     /translation="MSEQPQKIKVAIVEDDKEMREGLESIVKFHPGLRLLSSYPSAEE
                     ALAKISDEIPDIVLMDIHLPGISGIECVRQLKPVVPHTQFMMCTVYEDYENIFDSLCA
                     GATGYLLKNSPPGQIADKLFISFETVRTHIHNIFVKLHVQSRTEAVNKVFGR"
     gene            complement(6425..>7940)
                     /locus_tag="PKM34_02900"
     CDS             complement(6425..>7940)
                     /locus_tag="PKM34_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82562.1"
                     /translation="SISCVQFTDMAGLDSLKTIGGSLTFYDTQLTDMSGLGALETVGG
                     DFTIGCFTNTGLYCNNPYLENLSGLQDLQSIGGTLTVQYCESLTNIDDLAGLTQVSRI
                     YIKANPILNSVEGLANIYPDSLVSLTIAGNPSLNSCEVESLCSFLDDPAGQINIYDNG
                     TGCDNPVEIATACGVELSCLPYGNYWLLSQADINSFPQDFPDCNEINGTIYVIGGDIV
                     NLEPLNQVNSVHGALILGHEWYGGGNPVLENLQGLENIDSITGTLHIQYCPMLDDLSE
                     LQNLSFVGGTLQLVSNDGLTRLSGLEGLTSIGSGIDISANDRLKDLSGLENVTDVGSG
                     ILIRLNDSLASLAGIDNISAGSISLLNIYFNDMLSECDVASICEYLAAPNGEIDISGN
                     APGCNSPEEVLEECAVGVEESSVVGRRSSVVSYPNPTDGLTQFQISIFDFEHVSLTIY
                     NAQGQEVVVVLDEKMAAGEHTVRWDAGELPAGIYFYQVRAKSVGQVSAGKIVKY"
BASE COUNT         2289 a         1847 c         1738 g         2066 t
ORIGIN      
        1 caggtgatga catcgctgtt cggcaaccag cttaatatgg acgaagccaa caaaacgatc
       61 gttttcgaaa ggcaggggct gatcttcgtt tttaatttcc acccgacgca ttccattgct
      121 gattatgctt tcccggtgcc ccgccacgga gaataccgga tcatactgaa ttccgacagc
      181 aaagcgttcg gcggccacgg ccggatcgac gattcgatta cgtattatac cttttccgac
      241 ccatcaggca actggcctta cctgaagatc tataacacga accggacggc cctcgtgctg
      301 aagtttgatc tgtgaagcct ttaaaataga tcagaagacc ctattcaaca aggatctttc
      361 cggtcagaat gcctgaagcg gagacgattc tcaggaaata aatacccggc gtaacctcat
      421 ttccccactg gagggtcaac cggttcggac cgtcgaacag cattttgccg ggcatttgca
      481 caacggtcct tcccgacagg tcaaccagtt caacggttgc agggcctcct ttatccacgg
      541 tgaagttcat cgttaattcc ccgtgcgcgg gatttggaac ggctttcaac actgtcggat
      601 catccggcac ctccggcacc tccattgtcc cgttattgtt tgtgtacata accgttccat
      661 aggtcccgac cgcccagccg gtatcatcat cgataaagaa aatatcatac agactctctt
      721 ccggaggtag gttcatcatg tcccaggtat ttccgccatt gccggtatac ataaaaaatc
      781 cgtcaccgcc aaccgcccat ccggattgat ccgacagcag acaggcctca accgggaaga
      841 tttcatcgga gaagatttca tgccaggttt ctccgccgtc ctgggtagcc attataaagc
      901 cgaagtctgc ttcagccccg tgaatgcctt caatccagct ctcgccaaca agccagccaa
      961 ccgaatcgtt tgcaaatgcc atgtcgttgt aaaagcagaa aggatctgag caccagttta
     1021 atttcacctg ccaggttgcc cctccatcgg tggttttaat gatcctggcg cataaatcgc
     1081 ttccattgtg gtaagtgccg ccgataaacc ctgtcatcga atccgtgaaa ataacgtcat
     1141 atgggatctg aacctcatta ataatagcct ctgaccaggt cgccccgccg tcaaaggtcg
     1201 atctcaacat gcagggcagc ctttcgccgg attcaccgat catccagcca tgcagcgaat
     1261 ccgtaaagca aacatcctga aggttgtgta gcgaaacatc ctgaaggatc caatttgtac
     1321 caccgtcagt cgtttttaag atgtacccgc tcgtgtcggg gccatgaccg acaatccagc
     1381 cattgaggct gtcgatcatg gaaatgtcac caaatgaaac tccaaccggg caatattgaa
     1441 tctcccaggt gctcccgccg tcattcgtat gaagaattgt tcctgaatcc ccgaggatcc
     1501 acccgtgaaa ggaatcgata aaggtaatcc cggagagatt tgtactaaca gggatatcag
     1561 tgtgctggta ccactgtgaa tagactgata acgagaacag aaccagtgcc agggttaaca
     1621 ggaagttttt catggcgaaa ggaattattc ccaaatataa ggataaaagg gataaaaaca
     1681 tgttccgttt tacttttttc atttttcatt tatgcgtata aattataaat attgattata
     1741 tttgtaacaa aatatttata tgaaaccaat tactttcacc agcacccttc cggcagagat
     1801 ctggcaatcg ctggaagcct atgcggagaa gtttaaggtg ccccggaaca ggatcatgga
     1861 acaagccctg aaggcttatt ttgaaagact caaacaggcg gaatatatcc attcttttag
     1921 aaaagctgcc ggagatccgg aaatgggtgc aatggcagca gaaggactgg cagattatct
     1981 caagatcctt gatgaaccat gaaacagcgg gaaatatgga tggccgatct taacccggtc
     2041 aagggaagcg aacagcaggg aatacagccg gtggtggtta tcagcggcaa tgccatgaac
     2101 gataacctgg gaataggcat tatttgtccg cttacgtcaa aaataaagca ttacgccggt
     2161 tgcctggtct ttaaaaaaga tgatattaac aggcttgacg acgactccga gatcatcact
     2221 ttccaggtca ggacgatagc caaagagcga cttatctcca aaacaggaga gatcaccaaa
     2281 tctcaacttg aagctataaa gaaaggtctg caggagattc tgacttattg aagatggaag
     2341 tattggttcg gttgtaacac attcgtgaag atccgttgca ttcgcgccat tcgtgtgctg
     2401 tcaatagagt gaacacgaat gaaacgaata caacgaatga tcacggaata ttacgtaggt
     2461 tcatcatctt ggccatttaa gaaaacgata ttcttagtat ttcgcgatct ttcccatggg
     2521 atggactcgg ataactgacg actgacaacc gactacctgc aaaattacct ctccccgcca
     2581 tcgaaatcaa tacgtatttc cggggattct tgggattcca gtgttccaac atcagtacgc
     2641 aactattaac taacaaaaag attagttaca aaataattac ttatctttga ttaaaattat
     2701 aattatggac actcctttta tatttggaaa gctcgccaca ggtgaaaact tcacagaccg
     2761 ggaggctgaa caacaaaggc ttattcagaa tttccggtca gggaccaata ccattctgat
     2821 ttctccgcgc aggtggggca aatcttcgct tgtacataaa tctgcccagg aggctaagaa
     2881 agcggaacat gcgcctatca tggtatttat tgatcttttt aatatccgga gtgaggagga
     2941 tttttacaaa tgcctggctg aaagtgtgat ccgggctaca tcaggacgga tggaagagat
     3001 catgagtaat attaaggagt ttatgaagga gtgggttcca aggatcagtt tttccccgga
     3061 tgcccatcat gaattctccc ttagccttga ttgggctgag ataaagaaac aaccggatga
     3121 gatcctcaat ctggctgaga atattgccgt tgcaaaaaat cgcaaaatag tcatctgcct
     3181 ggatgagttt cagaacatcg ccttttatga aaatccactg gcctttcaga aaaagctccg
     3241 gtcgcactgg caaaagcatc agatggcttc gtattgtatt tacgggagca aacgccatat
     3301 gctgctcgat gtatttactt ctccatccat gcctttttac agattcggag acatgatgtt
     3361 cctgccgaag atttccatcg attactggat accctttata caagaacggt tcaaatcgac
     3421 gggaaaagac attgatcgtg agcaggcaga aaggattgca acattgaccg aatgccatcc
     3481 ctactatgtg cagcaactgg cacagcaaac ctggctcagg accgaaaaag ccgttacaat
     3541 cggtatcatt gatgaagcat tggacaatct tgtattacag atgagcctat tatttcagaa
     3601 tatcacggaa gatctgacca cttcctatgt gaatttttta aagatgatcc ttgacgggca
     3661 tacccgttat acgtcgatgg aaaatattga caggtatcaa ctcggcacat ccgccaacgt
     3721 tatcaggatc aggaaagcgc tgatcagcaa ggagataatc gatgaatatg aagggaaagc
     3781 cattctcctc gatccgctat acgccatctg gttaaagaag tattatttct aaggctatgt
     3841 gaagaaatcg ccttcagtat ttcacaatct ttcccattgt cgattgtcga tgttcgatgt
     3901 tcgatgttcg atagtaatag attcctgccg gcagcctgct catatcccac cggatcacct
     3961 gatcgccata acacatccca tccagcagcg acgccacttc ctgtccatgg gcgttataaa
     4021 tcttcaaggt cgcagattgt aaattgtaaa ccgtaaactg taattcgaca atcccatggg
     4081 tagggttggg ataaacgctg accgcaggct gtaagccgcc atctccctgc tccggaccga
     4141 ccccaaccga acaatcggga tctgtgaaaa tctcgacttt ggaagtcagg taaagtgaat
     4201 cgggatcctc ataaacaagc ccgccggcca ccagaaagtg atcctctgcc ccggcacagg
     4261 catgcatgac caggggattc gtcatatggt caactgacca ggtatctgtg tacgaatcat
     4321 agatatcgat cacatcagat ccggagttcc agcttatttg cgcctcccgt atttttgtct
     4381 ttcaattcca aattttagca aatgataatg aactaacttc taataataat agaaaaataa
     4441 atagaaatat ttgtattatg aatagaaata aaatacattt gtataaaata caaaccaaat
     4501 gagagacctt ttaaaaactg tcattgctga ccagcttacg ctcacctgga gtgagtcatt
     4561 caccagtaga aacgtaccgg aaacattgct gaaaggggaa gatatagtgg ttatttcggg
     4621 tataaggcgt tgcggtaaat ctaccttgct acaccagatc aggtcaggct attcggaaaa
     4681 ggattattat atgaatttcg acgacgagag gctactgaac ttcaaagtag aaaatttcca
     4741 gcttcttcat gaaactttta ttgaactgtt cgggcagcaa aaaactttct ggtttgatga
     4801 gattcaaaat gttaaaggtt gggaaaggtt tgtaagacgg cttcacgatt atggaaacaa
     4861 ggtattcatt acaggttcaa atgcaacaat gctcagcagg gaccttggta ctcatctgac
     4921 cggacgattt ctgaaacacg aactatttcc attttccttc agggaatttc tggatttcaa
     4981 caatattaag acgggaatcg acaatattta ttctactggc gagaaagcgg gaatcaaacg
     5041 gttgtttaat gaatatttta aaacaggagg gtttccatac tatcttaaat atgaagatga
     5101 taattacctg aaatcattgt acgaaagtat cctctaccgg gatatcatgg caaaacataa
     5161 tctaaccagt gagagggaac tgaaagaact gatttattta ctggccagca atgtatcccg
     5221 gccatattca aataacaggc ttgcagcttc aataggactg aaaaatgcca caacggtaaa
     5281 aaactatctc gattatattc aggatacata tctgttattt gctgtgaata aatttgatta
     5341 ttctgccagg aaacaactcc acaacgccag gaagatctat ttcattgata atgcgctgat
     5401 tcgtaagctt ggatttatgt tttcagaaga taagggaaga atgcttgaaa acctggtgtt
     5461 tatagaactg caaagaagag gcgaggaggt gttttattat aaagggaaag gagagtgcga
     5521 ttttgtcctc cgtgatggaa atagcatcac aggcgctgtc caggtaacct attccatgac
     5581 cgctaatgaa accagggaca gggaaataaa gggcttactg gaagcaatgg atagttataa
     5641 cctttcggaa ggtattattc tgactgatga tacagaagaa aacctgagtg tcaacggaaa
     5701 ccggatattg atcatgccgg tttataaatg gttgcttagg atctgaaaag aggtcatcgc
     5761 actgactact taacccttaa tcctcaatac ataacactat ctcccgaaca ccttgttcac
     5821 cgcttctgta cgcgactgca catggagttt cacaaaaatg ttgtgaatat gcgtcctgac
     5881 cgtttcaaaa ctgatgaaca gcttatcggc aatctggccc ggagggctgt tcttcagcag
     5941 gtacccggtg gcgccggcgc agagcgaatc gaatatgttt tcataatcct cgtaaacggt
     6001 gcacatcatg aactgggtgt ggggaacgac cggcttgagc tgccttacac attcaatccc
     6061 cgatattccg ggaaggtgaa tatccatcag cacgatatcg ggaatttcgt cggaaatctt
     6121 tgccagtgct tcttcagcgg aagggtagga ggagaggagc cggagcccgg gatggaactt
     6181 tacgatcgac tccagccctt ctctcatctc tttatcatcc tcgacaattg cgacttttat
     6241 tttctgtggc tgctcggaca tttcacaaag ttaaattgaa aacaagctga atgcaatacg
     6301 tatttcgggg gatattttgg ggtttcagag tttaagggtt ccaacgttta aaagttccag
     6361 tgttccggtg ctccagtgtt tcaacttgtc aaccgtccaa ccgtccaact gtccaactgt
     6421 ccaactaata tttcacgatc ttcccagcac taacctgccc cacgctcttc gccctaacct
     6481 ggtagaagta gatccctgct ggtagctcac ctgcatccca tcgcaccgta tgctctcccg
     6541 ccgccatttt ctcatcaagc actaccacca cttcctggcc ctgggcattg taaattgtca
     6601 aactcacgtg ctcaaaatcg aaaattgaaa tttgaaattg ggtcaatcca tccgtcggat
     6661 tcggataact gacgactgac gaccgacgac cgacgactga cgactcttca actcctaccg
     6721 cgcactcctc aagcacctct tccgggctgt tgcagcctgg ggcgtttcca ctgatatcaa
     6781 tttctccgtt aggtgcagcc aggtattcac aaatactcgc cacatcacat tcggaaagca
     6841 tatcattgaa atagatattt aataatgaaa tggaaccggc tgaaatatta tctattcctg
     6901 caaggctggc cagtgaatcg ttgaggcgga ttaaaatacc gcttcctaca tctgtcacgt
     6961 tttcaagccc tgatagatct ttaaggcgat cattcgcact gatatcgatg ccggaaccga
     7021 ttgatgtgag tccttccagc cctgataagc gggttaagcc atcattgctt accaactgca
     7081 atgtacctcc gacaaatgat aaattctgga gttccgagag atcatcgagc atcggacagt
     7141 actgaatgtg caacgtacct gttatggaat caatattttc aagaccttgc aggttttcca
     7201 ggaccggatt acctccgcca taccattcat gacccaatat cagggcccca tgaactgagt
     7261 taacctggtt taatggttcg aggttgacta tgtccccacc gataacataa atggttccat
     7321 ttatctcatt acaatccggg aaatcctgcg gaaaactgtt aatatccgcc tgcgacagga
     7381 gccagtaatt gccgtatggc aggcatgaga gctccacgcc acacgctgtc gctatctcga
     7441 cgggattatc acagcctgtt ccattgtcgt aaatatttat ttgccctgcc ggatcatcca
     7501 gaaagctgca caggctttca acctcgcacg aattcaggga aggatttcct gctatggtca
     7561 gcgaaaccag ggaatccggg tatatattgg caaggccttc aacagaatta aggattggat
     7621 ttgccttgat atagatcctg cttacctggg ttaatccggc aagatcgtca atattggtaa
     7681 gggattcgca atattgtacc gtcaatgtgc ctccgatact ttgtaaatcc tggagacccg
     7741 aaaggttttc caggtatgga ttattacaat acaatccggt attagtaaaa catccgatcg
     7801 tgaagtctcc gccaactgtt tccagggcgc ccagaccgct catatccgtg agctgcgtgt
     7861 catagaaagt cagactgcca ccgatcgtct tcagggaatc aaggccggcc atatccgtaa
     7921 attggacgca gctaatagac
//
LOCUS       DAOQXS010000060         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9601_length_7929_cov_3.548387, whole genome shotgun sequence.
ACCESSION   DAOQXS010000060 DAOQXS010000000
VERSION     DAOQXS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9601_length_7929_cov_3.548387"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKM34_02905"
     CDS             complement(<1..313)
                     /locus_tag="PKM34_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82563.1"
                     /translation="MLIKFFRSSYLIQYFALALITAALWIPGFRENQGLPVSPVIIAP
                     IYGMLHYVLSLHPVASPAFAIAIILVTAFALNNILIFHELTPKNNLLPAFLFIVLMSS
                     NP"
     gene            complement(343..849)
                     /locus_tag="PKM34_02910"
     CDS             complement(343..849)
                     /locus_tag="PKM34_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ82564.1"
                     /translation="MNLLTEPLNHLHKRNDFSCGNLLLDNYLKYQAGQDTRKKLSACF
                     ILSDHTTHQIKGYYTLSNSSLPTELFPEKLKLHFPRSYISIPAILLGRLAVDKTRQGK
                     GTGRLLLIDALRRSYLVSESIGSFAVVVDAISEEAGLFYDKFGFIMLPDSGKLFMPMK
                     TVKHLFER"
     gene            complement(846..1136)
                     /locus_tag="PKM34_02915"
     CDS             complement(846..1136)
                     /locus_tag="PKM34_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNQ82565.1"
                     /translation="MKAKTDKPEKARFDARLTREQKQIFERAAVIGGYRSLSDFVITI
                     AEERAQQIISKADSIIASQRDAEIFFDAVLNPGQPNEALRSAADEYNKLMAE"
     gene            complement(1184..2473)
                     /gene="purD"
                     /locus_tag="PKM34_02920"
     CDS             complement(1184..2473)
                     /gene="purD"
                     /locus_tag="PKM34_02920"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNQ82566.1"
                     /translation="MNVLIVGSGGREHALAWKIRQSPLLDKLYILPGNAGTDSLGENI
                     QLSVTDFKGIGRTVLEKQIDMVVVGPEVPLVEGIHDYFLADPVLKAIPVIGPVQKAAM
                     LEGSKDFAKEFLIRHHIPTARYRTFTRETYGNALEFLREMKPPYVLKADGLAAGKGVV
                     ICSDFGEAGTELQAMLMDEKFGEAGAKVVIEEFLSGIEMSAFTLTDGRHYLVLPPAKD
                     YKKIGEGDTGPNTGGMGSVSPVPFADDAFLKKVEERIIAPTHAGLLQEGIDYRGFIFF
                     GLINVGGDPYVIEYNARMGDPEAESVIPRIKNDLLELFAAVGNQTLHHHRIETVPGFT
                     ATVMLVSKGYPGNYEKNMEITGLDGIDGSLVFHAGTAIRPADGKVVATGGRVIAVTSF
                     GDSLTEAFGKSYENADRIRFENKSLRSDLGRDLLKFA"
     gene            complement(2485..3621)
                     /locus_tag="PKM34_02925"
     CDS             complement(2485..3621)
                     /locus_tag="PKM34_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ82567.1"
                     /translation="MKIAVNTRLLLKNKLEGIGWFSFETLKRITTQHPEHQFYFIFDR
                     PFDDSFIFSDNITPVVVPPQARHPILYYLWFEKSIPRVLKKIEPDLFFSPDGYLSLST
                     PVKSMHVIHDLNFEHYPDDLPYPERTYYRHYFPQYARKACRIATVSEFSKRDIINQYH
                     IGPEKIDVVYNGANDRYSPIPDSEKTLVRAKYSQGLPYFVFVGALHPRKNLANLFRAY
                     DLFRAETGINMKLLIIGHKKWWTRQIKESYEKMRFNGDVEFCGRLDPVELNRVISSAA
                     ALTYVSYFEGFGIPILESFYCETPVITSNVTSMPEVAGDAAILVDPFSVKSISEGLTK
                     IATDPELSRTLNEKARIRRQVFHWQRTADLLWNSIEKCLDTKKS"
     gene            3792..5279
                     /locus_tag="PKM34_02930"
     CDS             3792..5279
                     /locus_tag="PKM34_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ82568.1"
                     /translation="MQKKFLVNLAISLFLNLLVKPFWIFGIDRTVQNTVGLADYGFYL
                     NIFNFAFLFNILLDAGITNFNNRNIAQHVQLLNKHFSSLVVLKFLLTFVYIAVTFSIA
                     LVLGYRGDQLIMLAWVGFNQFLLSFILYFRSNISGLLMFMTDSVISVLDRVLMILICA
                     VLLWGNVTGGVFRIEWFVYAQTAAYMATLVIAAVIVMRKAKFRKLSWSRPFFLVILKK
                     SLPFAVLVLLMSVYNRVDTVFIERLVGGDEGERQVGIYAHAFRLLDALNQFAWLFAVL
                     LLPIYSRMIKLKEDVGRMTRLPYALLITSAIIVAMGSLFYRFEIMEWLYPKDSMQTAA
                     EYAGQHRQSATVFGILMFGFLGSTTMYVYSTLLTANGNLRQLNLVALAGIGVNFLVNI
                     LLVPRMQATGAAVASLATQLFTAGAYMALAHRIFRFKPDIAFLSRIVIFTLVVIFLNF
                     LCRQLRVDWKISFIVMMIASALSAFVLKLVNLPEMWRMVKERATP"
     gene            5411..6472
                     /locus_tag="PKM34_02935"
     CDS             5411..6472
                     /locus_tag="PKM34_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82569.1"
                     /translation="MTDPNNQESLKKDLNSLNLLHLLFKWRLPLFIITAVAIGAAVLF
                     SSPFFIKPKYKSSVIMFPVATNSISKVLISQNSGIKEDILGIGEEEQAEQMLQMLNSN
                     FIRDRIVQKYNLFKHYRIPENSKHKYTRLFREYENNVRFRRTEYMAVKITVFDTDPQI
                     AADMANDIAALLDSTRNMIQKERAVKAFRIVDEEYRSLENEVNGIVNQLKYLGSKGVN
                     DYERQSEVLNQQLAIALAQNNRHAITILQQKLDTIGKYGGEFLSLKNSLEFKTEQLTL
                     LKTKYQEAKVDAEQFLPQKFIVNSAYKAEVKSYPIRWIIVVVTAFIVFFTAVLIIIIV
                     ENYTGLQLKKNSGSRQYLS"
     gene            6511..7494
                     /locus_tag="PKM34_02940"
     CDS             6511..7494
                     /locus_tag="PKM34_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82570.1"
                     /translation="MERYFNNTNLINLLLKWRIHLLVILALAVVLSVFFSGPLFITPK
                     FKSFGVIYPANVSPYSEESETEQMFQVLQSQDIIDSVINKFNLAEHYEIAKEYKYYQT
                     TIYYEYSQNIKIEKTPYDAVRIEALDKDPVIASQIVEAIIGFYNAKVRYMHNEKYYEV
                     MDMYEKLLARKQSDIDSLKASLYKLMVESGLLGWDQSSEEIMRGYLRTVTGGAAPQIN
                     TAEVKRLKENMEKRGGDLILITERIKHEANSYSAFKVEYEDAMRFFNASLTYCNVVTK
                     PFPADKKSYPVRWLIVAMTFILTFFFAVVVILLVENLRVYQLRKNQNKDQS"
     gene            7518..>7929
                     /locus_tag="PKM34_02945"
     CDS             7518..>7929
                     /locus_tag="PKM34_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82571.1"
                     /translation="MYGASAVFIAANAYLFLHDKMWGLAIPFVLLLLILYIFSLDNVL
                     LLITFLTPLAVNIKDAEFGIGISLPSEPLMFGVMLLFILKLMHERFYDAKIMKHPVSI
                     AILINLVWLFITCFTSELPIVSIKFMVARLWFIIP"
BASE COUNT         1875 a         2008 c         2036 g         2010 t
ORIGIN      
        1 gcgggttgct gctcatcagc acgatgaaga ggaaagcggg cagcaggtta ttcttcggcg
       61 tcagctcatg gaagatgaga atgttattca gggcgaaggc ggtgaccagg atgatggcga
      121 tggcaaaggc cggactggcg acgggatgca gcgaaagcac gtagtgcagc atcccgtaga
      181 tgggggcgat gatgacaggt gaaacgggca gcccctggtt ctccctgaag ccggggatcc
      241 agagcgccgc agtgatcagc gccagcgcaa agtactggat aagatagctc gaccggaaga
      301 attttatcag cattacgatt aaacttttac caaatatcag gattatcttt caaataaatg
      361 cttgacagtt ttcatgggca tgaacaattt accgctatcc ggaagcatga taaacccgaa
      421 tttatcataa aatagccctg cctcttccga aatagcatca acaactactg caaatgaacc
      481 tattgattcc gagaccaggt aacttctccg gagtgcatca attaaaagca accggccggt
      541 tccttttccc tgccgggttt tatcgacggc caagcgaccc agcagtatgg caggtattga
      601 aatgtaggac ctgggaaagt gaagcttcaa tttttcagga aatagctctg tggggaggct
      661 gctgtttgaa agtgtgtagt atcctttaat ttgatgggtg gtgtgatcac tcaggataaa
      721 acaggctgac agtttcttcc gcgtgtcctg gccggcctgg tactttaaat aattgtcgag
      781 caggagatta ccacatgaaa aatcattcct tttgtggaga tggttcagcg gttccgtcag
      841 gaggttcatt cggccatcaa cttattgtat tcatccgcgg ccgatcttaa agcctcattg
      901 ggctggccgg gattgagaac agcgtcaaaa aagatttcag catccctttg cgaagcgatg
      961 atgctgtcgg ccttactgat tatctgctgt gccctttcct ctgctatcgt tatgacaaaa
     1021 tcggaaaggc ttctgtaacc tccgattacc gcagccctct caaagatttg cttttgctcc
     1081 ctggtcagcc gtgcatcgaa cctggccttt tcgggcttat ccgtttttgc tttcatgata
     1141 atttttttgc aaatgtacgt aatatatccg tacatttccg gattcaggca aattttagca
     1201 aatcccgccc caggtcgctt ctgaggctct tgttttcaaa cctgatcctg tcagcgtttt
     1261 cgtacgactt tccgaatgct tccgtcagcg aatcgccgaa agaggtcacg gcaatgaccc
     1321 ttcccccggt agcgaccact tttccatcgg ctggccggat ggcggtgcct gcatgaaaaa
     1381 caaggctgcc gtcgatcccg tcgagaccgg tgatttccat gtttttttca tagttccccg
     1441 ggtagccttt cgacaccagc atgaccgtgg ccgtaaatcc gggaacggtc tcgatccggt
     1501 gatggtgaag cgtttggttt ccaaccgccg caaagagttc cagcaggtcg tttttgatcc
     1561 tggggatgac cgattcggcc tccggatcac ccatccgggc attgtactca ataacgtaag
     1621 gatctccacc gacgttgatc aggccgaaaa agatgaaccc gcggtaatcg atgccctctt
     1681 gtagcaaacc ggcgtgggtc ggggcgatga tccgttcttc cacttttttc aggaaagcat
     1741 cgtcggcgaa aggcacaggg gagaccgatc ccatcccgcc tgtattcggc ccggtgtcgc
     1801 cttcgccgat cttcttgtaa tccttggccg gggggagcac caggtagtgc cggccgtcgg
     1861 tcagcgtaaa ggccgacatt tctatcccgg aaaggaattc ttcgatcact acctttgcgc
     1921 cggcctcgcc gaatttttca tccatcagca tcgcctgcag ttccgttccg gcttccccga
     1981 aatcgctgca gatcaccacc cctttgccgg cggcgaggcc gtcggctttc agcacatagg
     2041 gcggtttcat ctcccggagg aattccagcg cattgccgta ggtctcccgg gtaaaggtcc
     2101 ggtaccgggc cgtcgggata tgatgacgga tgaggaattc cttggcgaaa tccttgctcc
     2161 cttcgagcat ggccgccttt tgcaccggcc cgatcacggg gatggccttt aagacgggat
     2221 cggccaggaa atagtcgtgg atcccttcta ccagcgggac ttccgggccc accaccacca
     2281 tgtcgatttg cttctccaga acggtgcggc cgatgccctt gaaatcggtt acggagagtt
     2341 ggatattttc tccgaggctg tcggtacccg cattgccggg gaggatgtac aatttgtcaa
     2401 ggagcgggct ttgcctgatc ttccaggcca gcgcatgttc acgtccgccc gatcctacga
     2461 tcaatacgtt catctttaca gggtttagga ttttttggtg tcgaggcatt tttcgatgga
     2521 attccagagc aggtcggcgg tgcgctgcca gtggaatacc tgccggcgga tgcgcgcttt
     2581 ctcgttcagt gtgcggctca gctccggatc ggtggcgatc ttagtcaacc cttcactgat
     2641 cgatttcacc gagaacggat cgaccaggat ggccgcatcg ccggccacct cgggcatcga
     2701 ggtgacgttg gaggtgatca ccggcgtctc gcagtagaac gattccagga tcgggatgcc
     2761 gaaaccctcg aaataggaga cataggtcag cgccgctgcc gaggagatga cccggttcag
     2821 ttccaccgga tcgagcctgc cgcaaaattc cacatcgccg ttgaacctca ttttctcata
     2881 ggattctttt atctgacgtg tccaccattt tttatggccg atgatcagca gcttcatatt
     2941 gatgcccgtt tcggcgcgga acaggtcgta agcccggaac aggttggcca ggtttttccg
     3001 cggatgaagg gcgcccacaa aaacgaaata gggaaggccc tgtgaatatt ttgccctgac
     3061 cagcgttttt tccgaatcgg ggatcgggga gtagcggtcg ttggcgccgt tgtaaaccac
     3121 gtcgattttt tcgggcccga tgtggtactg gttgataata tcgcgcttgg agaattcgga
     3181 aaccgtggcg atccggcagg ctttccgggc gtattgcggg aaatagtgcc ggtaatacgt
     3241 ccgctcgggg tatggcaggt cgtcagggta atgttcgaag ttcaggtcat ggatgacatg
     3301 catcgatttt accggggttg aaagtgacaa atagccatcg ggtgaaaaga acagatcggg
     3361 ctcgattttc ttcagaacgc gcgggatcga tttctcgaac cagaggtaat acaggatcgg
     3421 atgcctggcc tgcgggggaa cgacgacagg agtgatgtta tccgaaaaaa tgaacgaatc
     3481 gtcgaacgga cggtcgaaaa tgaagtaaaa ctgatgttcg gggtgttgcg tcgtgatcct
     3541 tttaagggtt tcaaaggaaa accatcctat cccttccagc ttgtttttaa ggagtaacct
     3601 cgtgttaacc gcgattttca aaactgtgtg gtttgttgga aataaacccg gccggaacat
     3661 atcggttctg gcctgttcat cgttctttaa ttctcataac tttgtacaaa aataatttat
     3721 tttgaaaagg agggctaaaa agtgggcagc caaaaccgcc ggtacttttc agaagtttca
     3781 aacaagctgc gatgcagaag aaattcctgg tcaacctcgc catttcgctg tttttaaatc
     3841 tgctcgtcaa gccgttctgg attttcggga tcgaccggac ggtgcagaac accgttgggc
     3901 tggccgatta cggattttac ctgaacattt tcaacttcgc tttcctgttc aatattttgc
     3961 ttgatgcggg catcactaat tttaacaacc gcaatattgc ccagcacgtt cagttgctaa
     4021 acaagcattt ctccagcctg gtggtgctga agttcctgct gacctttgtt tatattgcgg
     4081 taactttttc cattgccctg gtcctggggt accgcggcga tcagctcatc atgctggcct
     4141 gggtggggtt taaccagttc ctgctgtcgt tcatcctcta tttccggtcg aatatttcgg
     4201 gactgctgat gtttatgacc gacagtgtga tttcggtgct cgaccgggtg ctgatgatcc
     4261 tgatctgtgc cgtgctgctc tggggaaatg tgaccggcgg cgtgttccgg atcgagtggt
     4321 tcgtgtatgc ccagacggcg gcctacatgg ccacgctggt tattgcggcg gttatcgtga
     4381 tgcggaaggc gaagttccgg aagctgagct ggagccggcc ttttttcctg gtgatcctga
     4441 agaaaagcct gccgttcgcc gtgctggtgc tgctcatgtc ggtctacaac agggtcgaca
     4501 cggttttcat cgagcggctt gtgggcgggg atgaagggga acggcaggtg gggatctatg
     4561 cccacgcatt ccgtttgctc gacgcgctga accagtttgc ctggctcttt gccgtgctgt
     4621 tgttacccat ttattcgagg atgatcaaac tgaaggagga tgtgggccgg atgacccggc
     4681 tgccgtatgc gctgctgatc acctcggcga tcattgtggc aatgggttcg ttgttttacc
     4741 ggtttgagat catggaatgg ctgtatccga aagacagtat gcagacagcc gctgagtacg
     4801 caggtcagca ccgccagtcg gcaaccgttt tcggcatcct gatgttcggt ttcctgggaa
     4861 gcaccacgat gtatgtttac agcaccctgc tgacggccaa cggcaacctc cggcagctca
     4921 acctggttgc gctggcgggt attggggtga acttcctggt caatattttg cttgtgcccc
     4981 ggatgcaggc caccggcgcg gcagtagcaa gcctggcaac ccagttgttc accgcggggg
     5041 cttacatggc cctggcccac cgcattttcc gttttaagcc ggatatagct ttcctttccc
     5101 ggattgtcat ctttaccctc gtcgtcattt tcctgaattt cctttgccgg cagctccggg
     5161 tcgactggaa gatcagcttc atcgttatga tgattgcatc ggccctttcc gctttcgtcc
     5221 ttaaactggt caaccttccg gagatgtggc ggatggtgaa agagagggca acaccataac
     5281 aggcgaacag acgaacaggc ggagtatgct gttattgcac caattatctt ttttcactta
     5341 actttctgat aacccatcat cattaaatat ttattcctac atttgacctc ttttttggaa
     5401 aaagctgaac atgaccgatc cgaataacca ggaatcgctg aaaaaagacc tgaattccct
     5461 taacctcctt cacctgcttt tcaagtggag attgccgctg ttcatcatta cggccgtcgc
     5521 catcggtgcc gccgtcctct tttcatcgcc tttcttcatc aaaccgaaat acaaatcatc
     5581 ggtgatcatg ttcccggtcg ccaccaactc catctccaaa gtcctgatca gccagaactc
     5641 aggaataaaa gaggatatcc tcgggatagg agaggaggaa caggccgaac agatgctcca
     5701 gatgctcaac tccaacttca tccgcgaccg gatcgtgcag aaatacaacc tgttcaaaca
     5761 ttaccgtatt ccggaaaatt caaagcataa atatacccgc ctgttccgtg aatacgaaaa
     5821 caacgtgagg ttccgccgga cggaatatat ggcagtgaaa atcaccgtat tcgataccga
     5881 tccgcaaata gccgccgata tggccaacga tatcgccgct ttgctcgact ccacccgcaa
     5941 tatgatccag aaagaacgcg ccgtcaaagc tttccggatc gtcgacgaag aataccggag
     6001 cctcgaaaac gaagtcaacg ggatcgtcaa tcagttgaag taccttggca gcaaaggggt
     6061 gaacgattat gagcggcagt cggaggtgct taaccagcaa ctggctatcg ccctggcaca
     6121 gaataaccgg catgccatta cgatattgca gcagaaactg gatacgatcg gaaaatacgg
     6181 cggcgagttc ctgtcgctga aaaattccct cgaatttaaa acggagcaac taaccctgct
     6241 aaaaacaaaa taccaggaag ccaaggtgga tgcggaacag ttcctgccgc agaagtttat
     6301 cgttaacagc gcctacaaag cggaagtcaa atcgtacccg atacggtgga taatagtggt
     6361 cgtgacggct tttatcgtct tcttcaccgc ggtactgatc atcatcatcg tcgagaacta
     6421 caccgggtta cagttaaaaa aaaactccgg atcccggcaa tacctaagct gaaagagagt
     6481 attcagaata taattttatc tgagttaaaa atggaaagat actttaataa tacgaacctg
     6541 atcaacctgc tgctgaaatg gcggatccac ctgctggtga tcctggccct ggcagtcgtg
     6601 ctgtcggttt tcttctccgg cccgttgttc atcaccccaa aattcaaatc tttcggtgtt
     6661 atctacccgg ccaacgtttc gccgtattcc gaagagagcg aaaccgaaca gatgttccag
     6721 gtccttcaat cacaggatat tatcgatagc gtcatcaata aattcaatct tgccgaacat
     6781 tacgaaattg ccaaagaata taaatattac cagacaacca tctattacga atacagccag
     6841 aatattaaga tcgagaaaac cccctacgat gcggtgagga ttgaagccct tgacaaggat
     6901 cccgttatcg ccagccagat cgttgaagcc attatcggtt tctacaatgc caaagtgaga
     6961 tacatgcaca acgagaagta ctacgaagtg atggacatgt acgaaaaatt gctggccagg
     7021 aaacaaagcg acattgattc attgaaggca tcactctata aactgatggt cgagtcgggg
     7081 ctgctggggt gggaccagag ttcggaagag atcatgaggg gttacctgcg caccgttacc
     7141 ggcggagccg ccccgcagat caacaccgcg gaggttaaac ggctgaagga gaatatggaa
     7201 aaacgaggag gcgacctcat cctgatcacc gaaaggatca aacatgaagc caactcctat
     7261 tcggctttta aggtcgaata tgaagatgcc atgcggttct tcaatgccag cctgacctac
     7321 tgcaacgtcg ttaccaagcc gtttccggcc gataaaaagt cgtacccggt acgctggctg
     7381 atcgtggcca tgacattcat ccttacattt ttctttgccg tggtggttat cctgctcgtt
     7441 gaaaacctcc gcgtttacca gttaagaaag aatcaaaaca aagatcagag ttgaccgaaa
     7501 gggccaaatt aatctgggtg tacggcgcca gcgcggtctt tatagcggca aacgcctacc
     7561 ttttcctgca tgataagatg tgggggctgg ctatcccttt cgttctgctc ctgcttatcc
     7621 tctatatctt ttcgctcgat aatgtcctgc tgctcattac tttcctgacc ccgctggcag
     7681 tcaacatcaa agacgccgag ttcggaatag gtatatcgct tccttcagag cccctgatgt
     7741 tcggcgtcat gctgctgttc atcctgaagc tgatgcacga gcgcttttac gatgcgaaga
     7801 tcatgaagca tccggtcagc attgctattc ttataaacct ggtatggctt ttcatcacct
     7861 gcttcaccag tgaattacct atcgtttcga ttaaattcat ggtggccagg ctctggttca
     7921 tcatcccgt
//
LOCUS       DAOQXS010000061         7861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9682_length_7861_cov_3.147963, whole genome shotgun sequence.
ACCESSION   DAOQXS010000061 DAOQXS010000000
VERSION     DAOQXS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9682_length_7861_cov_3.147963"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="PKM34_02950"
     CDS             <1..1561
                     /locus_tag="PKM34_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ82572.1"
                     /translation="LFRERPGTQHQSSIDGTLQEKALEVAGRHQRTLQGNRIYNAAVL
                     VIDLNTNEVLVYIGNTAGSNEPEHSPDVDIIMAPRSSGSILKPFLYAVMMDGGDLLPT
                     TLVPDVPMFYSGFSPKNFSLTYDGAVPARKALSRSLNIPAVYMLREYGVQPFYDKLQK
                     LGMTTLTKSASHYGLSLILGGAEVRLWDVCRMYAGMGRTLKAYAERERDYRGVQGPEP
                     GTPSLGTPSPVYSAAAAWLTLDALRDVNRPEEEEGWQSFASSREIAWKTGTSFGFRDA
                     WAVGMTADYLVGVWAGNADGEGRPGLTGVSAAAPLMFEIFRLLPPSRHWFLQPYDEME
                     KVAVCRQSGFRPSLVCPDIDSVYVARKGLNSLTCPYHRLVHLDESRTSRVNADCYPAD
                     RIQSIPWFVLPPAMASYYRFRDPFYKPLPPLMDGCSDGNRSAMQMIYPSGDKKILIPK
                     GADGKPGEVVFEAAHLVPSAIIYWHLDNEYVGSTRDIHKMSLQPAPGEHYIILVDENG
                     ERLATKIEILP"
     gene            1783..3708
                     /locus_tag="PKM34_02955"
     CDS             1783..3708
                     /locus_tag="PKM34_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82573.1"
                     /translation="MARITRKWENGYVNPDWAESERISRRLASKITLAANAFYLTFGF
                     IFFFFINLQAPQIYAGPVLPHLKSGTENLITIDGHVTHMIQGQPSNYHPPTDVLFYYP
                     GETEPFDQTQTDPNGDYTSTMNYVTVSENNELVKGMKVLGNPYTDKAKLEIGIQEAGK
                     YSINVYDASTGKLLLSQDSELPESNTNISLSGLGSAGVKIVSVTNGEDIYTAKLVQVY
                     SNQFSPDIAINSTPIERNNLKSLEFLTDSLVIRFVTSNSYPEYYTLEKTVEAVTSTVD
                     ATMQQMPVTYDFLAYGYNINEGESVGNNASLKVTWGDGTSNIYTSDSEGRIHILRENT
                     LDTARNIFIENADTTYFQEWIFGVKQENLITTKEKNLFQSQKEQSGAPGTSYVAPTPA
                     PANLTMFPDTFEVYFVSKIVYDSEGQAHNTRGEVFRTVVSCRSNPDLTKKWEIGQNVT
                     TYLYEMQTYESKKNAIDPKTFDLGKYLEEQFSNRETLEYITPEQSALQSQALDSLIAQ
                     GFYLRQNGRQLFPEFERVLVYDGTEPEYLEGQARGWDQTHFSQYGNNNENGCVFTSDF
                     TFSDYYRFKGAIANYTSTSNMSYKMDELIGSFTHSRDPPNGNLSGKVTFTDGHLTSFG
                     NGVIHVIYIADPGTIFW"
     gene            complement(3869..5107)
                     /locus_tag="PKM34_02960"
     CDS             complement(3869..5107)
                     /locus_tag="PKM34_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82574.1"
                     /translation="MIERKFYLGKIKSGFAVVPIVLLIGARQVGKTSLMKMFNFNGAS
                     LFLNGQDPEISGLFERLNSIEDYIKVYLNPDLTGLLMIDEFQFIPGISTMLKLLTDKN
                     EGLKILCSGSSSLDIQQTVEESLAGRIRVIEVLSLSFEEYLKFTREKLHMLYENFGLT
                     TPDSALTAPFHQALKEYMLYGGLPRAALIHDYEQKLAILDDIYKTYLVRDVRSYIKNE
                     HFTGFNRLLKMLASQVGNMVNINSISRETGLPYRACDEYMDLLQQMYIIKLIAPFSSN
                     KRSAITKMKKIYFTDLGLRNMISSGFQEMEYRADKGALFENFILLELFRNLKPGGNIY
                     YFRTTDGTEVDFIVQNPTSSYAVECKYAILQKPLKIRALNSLSGDENLINCYIINQNF
                     NFSEGTTHHIQGYLAGFINR"
     gene            complement(5280..6671)
                     /gene="fumC"
                     /locus_tag="PKM34_02965"
     CDS             complement(5280..6671)
                     /gene="fumC"
                     /locus_tag="PKM34_02965"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNQ82575.1"
                     /translation="MNTRTEKDTMGLVEVPAEKYWGAQTQRSVMNFKIGDPGSMPVPI
                     IRAFGILKKAAALTNFELGVLPKEKCDIICQVCDEIIAGKLDDNFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLLGNKLGEGTRFIHPNDDVNKSQSSNDTFPAAMSIAGYKMIVET
                     TIPGVEKLKQTLEEKSAGFMDIVKTGRTHLMDATPLTLGQEFSGYAAQLGYGIKALKN
                     TLPHMSELALGGTAVGTGINTPKGYSELVAKKIAELTGFPFVTAPNKFESLSAHDAVV
                     ESSGALKTLAVSLMHIASNIRLLSSGPRCGYSEIILPANEPGSSIMPGKVNPTQPEAM
                     TMVCAQVLGNDVAINIGGTHGHFQLNVFKPVMIANLLQSARLLGDACVSFNDHCVIGI
                     EPNLPNIKKNMENSLMLVTSLNTKIGYDNAALIAKKAHTENKTLREAAIESGLVTGEQ
                     FDEWVDPRKMIGR"
     gene            complement(6747..7839)
                     /gene="prfB"
                     /locus_tag="PKM34_02970"
     CDS             complement(join(6747..7775,7777..7839))
                     /gene="prfB"
                     /locus_tag="PKM34_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131840564.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNQ82576.1"
                     /translation="MITQEFYKNLNQRLDALRRFLDVEKKKQTITEKEELTHAQDFWN
                     DPKKAEEVLRSIRDIKRWTDAFDEAKASFDDLVVIYEFYEAGEATDEDLDRQAEKTTK
                     IIENLEFLNMLSREEDKLSAILQINSGAGGTESQDWVAMLMRMYIRWAEIQGHKVKEL
                     DRQDGDVAGIKSVSIEIDGEYAFGYLKGENGVHRLVRLSPFDSANRRHTSFASVYVYP
                     MVDENIQVEINPADITWDTFRSSGPGGQNVNKVETAVRLKHEPTGIIVECQETRSQLQ
                     NREKAIQMLKSQLYEIELRKKREAQAEIESKKKKIEWGSQIRSYTLHPYKLVKDHRTD
                     LEAGNAQAVLDGEIDEFLKAFLMEFGGSQ"
BASE COUNT         2046 a         1842 c         1828 g         2145 t
ORIGIN      
        1 gcttttccgg gagcgtcccg gaacgcagca ccaaagttct atcgacggta cgttgcagga
       61 aaaggcgctg gaggtggccg gccggcatca gcggacattg caggggaatc gcatatataa
      121 cgctgccgtt ctggtcatcg acctgaatac gaatgaagtg cttgtctaca ttgggaatac
      181 agccggcagt aatgaacctg agcacagccc ggatgtagac attatcatgg cgccccgcag
      241 cagcggcagc attttgaagc cttttcttta cgcggtgatg atggatgggg gcgatttgct
      301 gccgacgacg ctggtgccgg atgtgccgat gttttattcc ggattttcgc cgaagaattt
      361 cagtctgact tatgatggcg cggtgccggc ccggaaggct ctgagccggt cgttgaacat
      421 tccggccgtt tatatgctcc gggagtatgg agtgcagcct ttttacgaca agttgcagaa
      481 gctgggcatg acgacgctga ccaaatccgc gtcacattat gggttgtcgt tgatactggg
      541 gggtgcggag gtgcggttat gggatgtatg caggatgtat gcggggatgg ggcggacgct
      601 gaaggcttat gcggagcggg agagggatta ccgcggggta cagggccctg aaccagggac
      661 gccatccctt gggacgccat ccccagttta ctccgccgcc gccgcctggc tcaccctcga
      721 cgccctgcgc gacgttaacc ggccggagga agaggaaggt tggcaatcgt tcgcctcttc
      781 ccgcgaaata gcctggaaaa ccggtaccag cttcggattc agagacgcat gggctgtggg
      841 tatgaccgcc gactatctcg tcggtgtctg ggccggtaat gccgatggcg aaggacgccc
      901 cggcctcacc ggcgtctcag ccgccgcccc gctgatgttt gaaatctttc gcctgctgcc
      961 gccttcccgg cactggttcc tgcaacccta cgatgaaatg gagaaagtgg cggtatgccg
     1021 gcagagcggc ttcaggcctt cattggtctg ccccgacatc gattcggtgt atgtggccag
     1081 aaaaggtctt aattcgctga catgccccta ccatcgcctt gtgcatctcg acgaatcacg
     1141 cacatccagg gtgaacgccg attgctaccc ggctgacagg attcagtcaa tcccatggtt
     1201 cgtccttccc cccgccatgg cttcatacta ccggttcagg gatccgttct ataaaccgct
     1261 gcctcccctg atggatggat gttcggatgg aaaccgttcg gcgatgcaaa tgatctatcc
     1321 ttcgggagat aaaaagatcc ttatcccgaa gggcgctgac ggaaaacccg gtgaagtcgt
     1381 gttcgaggcg gcacacctgg tcccttcggc catcatctac tggcacctcg acaatgaata
     1441 cgtgggctca acccgggata tccataaaat gagcctccag cctgctcccg gcgaacatta
     1501 tataattctt gtggatgaaa acggagaaag gctggctacg aagatcgaaa tccttccgtg
     1561 atcgggcatt ttagcaaaag tatttgtttc ttatctttga ttgaaattga cctttcttaa
     1621 ccaaccagat ataaaatcat gcagcatccc ggtacaataa aagtcagttc ctgcccaagg
     1681 gttaaacatg agctctcgcg aaaaaatcat aagaggagac ctgtttataa gcaggtgagc
     1741 atcagtgctg aagaacgtgc gttacgatca ctcaaatacc gcatggcgcg tattacccga
     1801 aaatgggaaa atgggtatgt taatccggat tgggccgagt cggaaaggat cagccggcgg
     1861 ctcgcatcga agatcacact ggcggctaat gcattttacc tgacttttgg attcatcttc
     1921 ttctttttta ttaacctcca ggccccacaa atatatgccg ggccggtctt accgcatttg
     1981 aaaagcggca ctgagaattt gataacaata gacggtcatg ttacacacat gattcaaggt
     2041 cagccttcta attatcatcc gcctacagat gtgttgttct attatcctgg agaaacagag
     2101 ccgtttgatc agacgcagac agacccgaac ggggactaca caagtactat gaactatgtt
     2161 actgtttcag agaacaatga acttgtaaaa ggaatgaaag tattaggaaa cccttacact
     2221 gataaagcaa agttagaaat tgggattcag gaagcaggca aatacagcat aaatgtttat
     2281 gatgctagca ctggaaaact tttgttaagt caggacagcg agctgcctga aagcaataca
     2341 aatatttcac tctcaggatt aggaagtgca ggagtaaaaa tagtaagcgt tacaaacggt
     2401 gaagacattt atactgcaaa actagtgcag gtttatagta atcagttttc tccagatata
     2461 gcaataaatt ctacgccaat agaaagaaac aatctgaaaa gtctggagtt tcttacagac
     2521 agtttagtga taagatttgt aacctcaaat tcataccctg aatattatac tttagaaaaa
     2581 acagtagaag cagtaacctc aacagtagat gcgacaatgc agcaaatgcc agtcacttat
     2641 gacttcctgg cgtacggata caatataaac gaaggagaat cagtaggaaa caacgcatca
     2701 ttaaaagtaa cctggggaga cggaacaagt aacatttata cttcagattc tgaaggaaga
     2761 attcatattt taagagaaaa cacattggat actgctcgga atattttcat agaaaacgca
     2821 gacacaacct acttccagga atggatattc ggagtaaaac aggaaaacct aataacaacc
     2881 aaagaaaaaa acctatttca aagccagaaa gaacagtcag gcgcaccagg aacgtcatat
     2941 gttgcaccta ctcctgcgcc agcaaattta accatgttcc cagacacatt tgaagtttat
     3001 tttgtttcaa aaatagttta tgactcagaa ggccaggcac ataatacgcg aggagaagtc
     3061 ttcagaacag tagtctcttg tagatcaaat cctgatttaa ctaaaaaatg ggaaatagga
     3121 cagaatgtaa caacttatct ttacgagatg cagacatatg aatcaaagaa gaatgcaata
     3181 gacccaaaaa ccttcgacct aggtaaatat ttagaagagc aatttagcaa tagagaaaca
     3241 ctcgaatata ttactcctga acaaagtgct ttgcagtcgc aggcacttga ttctttaatt
     3301 gcacaaggat tttacttgcg acaaaatggc cgccagttgt tccctgagtt tgaaagagtg
     3361 ttggtttatg atggaacaga accagaatac ttggaaggcc aagccagagg atgggaccag
     3421 actcacttct cgcaatatgg aaacaataat gaaaatgggt gcgtttttac atcagatttt
     3481 acattcagtg attattatag atttaaaggt gcaatagcaa attatacctc aacaagcaat
     3541 atgagttata agatggatga gctaataggg tcatttacac attcaaggga tcctccaaat
     3601 ggcaatttga gtggaaaagt tacttttaca gatggtcatc ttactagctt tggtaatggg
     3661 gttattcatg ttatttatat tgcagaccct ggcacaatat tctggtaaat tataatagaa
     3721 taaaaaataa ttttatttag tctacgtaat atggttgtcc ttcagctatt ggtaaactgc
     3781 aggtgcaggt tgttgtgtct acattcatgc agtttgatgt gcagcttgga gcgcctgtga
     3841 gaacagttcc gcctgtgttc ggtcctgatc aacgatttat aaatccagcc aggtaaccct
     3901 ggatatggtg tgttgtccct tcgctgaaat tgaaattttg attgataata taacagttta
     3961 ttaaattttc atcgccactt aagctattaa gagcgcggat tttaagaggt ttttgaagga
     4021 ttgcatactt acattccaca gcatagcttg aagttggatt ttgcactata aaatccactt
     4081 cggttccatc agttgtcctg aaatagtaaa tgttgccccc gggtttcagg tttctaaaaa
     4141 gctccaacag gataaaattt tcaaaaagcg ctcctttatc ggcacgatac tccatttcct
     4201 gaaaaccaga gctgatcata tttcttaatc ccagatcagt gaaatatatt ttcttcatct
     4261 ttgtaattgc cgaacgttta ttagatgaaa aaggcgcaat cagtttaata atatacatct
     4321 gctgcagtaa atccatgtat tcgtcacaag ccctgtaagg cagacctgtt tcacgactta
     4381 tcgaattaat attcaccata tttccaacct gggaagctaa catcttcaac agcctgttaa
     4441 atcctgtgaa atgctcgttt ttaatatagc ttcgaacatc gcgaacaagg taagttttat
     4501 aaatatcatc caggatggca agtttttgct cataatcatg gatgagcgca gctcgtggca
     4561 atccaccata aagcatatat tctttaagtg cttgatgaaa tggcgctgtt aatgcagaat
     4621 caggcgtagt aagcccaaaa ttttcataaa gcatatgaag tttttccctg gtaaatttaa
     4681 ggtactcctc aaatgaaagc gataagactt ctattaccct gattcttccg gcaagtgact
     4741 cttccacggt ctgctgaata tcaagacttg aactacctga acaaaggatt ttcaatcctt
     4801 cattcttatc tgtgagtaat ttaagcattg tagaaatgcc cgggatgaat tgaaattcat
     4861 cgatcattag tagtcctgtc aaatcaggat tcaggtagac cttaatatag tcttcaatgg
     4921 aattcagtct ctcaaacaac ccggatattt ccggatcctg cccgttcagg aataacgatg
     4981 cgccattaaa attaaacatc ttcatcaggc tggtcttccc aacctgccgg gctccgatga
     5041 gcaaaacaat aggtactacg gcaaaaccgc tttttatttt acccaggtag aattttcttt
     5101 caatcattat tcaagtttta aaatcctggt aaagatacat taaataatga tttaaatcca
     5161 gttattttac attaatgata atattatagc aatatatttt acgtttatgg taaataactt
     5221 gtgattttca atatttttat aagatccgga aattttatcc gggaattacg ataaaatcgt
     5281 tatctcccaa tcatcttcct cggatccacc cattcatcaa actgttctcc cgttaccaga
     5341 ccgctttcaa tcgctgcttc gcgaagcgtc ttgttctcgg tatgcgcctt cttggcgatc
     5401 agggcggcat tgtcgtagcc gatcttggtg ttcagtgagg ttaccagcat cagtgagttt
     5461 tccatgttct tcttaatatt cggaagattg ggctcgatgc cgataacgca atggtcgttg
     5521 aatgagacgc aggcgtcgcc cagcaggcgg gccgattgca gcaggttggc gatcatgacc
     5581 ggtttgaaga cgttcagctg gaaatggccg tgcgtgccgc caatattgat ggcgacatcg
     5641 ttaccgagca cctgtgcgca gaccatcgtc atcgcttcgg gctgggtcgg gttgaccttg
     5701 ccgggcatga tcgacgagcc gggttcgttg gccggcagga tgatctcgct gtagccgcat
     5761 cggggcccgg aggagaggag acggatgttg cttgcaatgt gcatcaggct gacagccagg
     5821 gtcttcagtg cgccgctcga ttcgactacc gcgtcgtggg ccgataagga ttcgaacttg
     5881 ttcggcgctg tcacgaacgg gaatccggtc agttcggcta ttttcttggc caccagctcc
     5941 gagtaacctt tcggcgtatt gatcccggtt ccgaccgctg ttccgcccag cgccagctcc
     6001 gacatgtgcg gaagcgtgtt cttcaacgct ttaatcccat agccaagctg tgccgcatag
     6061 cctgagaatt cctgtcccag cgtcagcggt gtggcgtcca tcaggtgagt gcggccggtc
     6121 tttacaatat ccatgaaacc agccgatttt tcttccagcg tctgtttcag cttttccaca
     6181 ccgggaattg tcgtttccac gatcatttta tacccggcga tgctcatggc tgccggaaac
     6241 gtatcgttcg acgactgcga cttgttcaca tcatcattcg ggtggatgaa ccgtgtccct
     6301 tcgccgagtt tattgcccag caaaacatgg gcccggttgg ctaccacttc gtttacgttc
     6361 atgttcgact gcgtccctga accggtctgc cagatcacca gcgggaaatt gtcgtcgagt
     6421 tttccggcga tgatctcatc gcatacctgg cagatgatat cgcatttttc tttcggcagg
     6481 acgcccagtt cgaaattggt cagggccgcg gcttttttca ggatgccgaa agcccggatg
     6541 atcggtaccg gcatcgatcc cggatcaccg attttgaagt tcatgacaga tcgctgggtc
     6601 tgtgctcccc agtatttttc ggccggcacc tccaccaggc ccatcgtgtc tttttctgtt
     6661 cttgtgttca tgggttgtta tttggtttga taaattgtta aattgataaa ttgttatact
     6721 gtccaactgt ccaaccgtcc aactgcctac tgactcccgc cgaactccat caaaaaggct
     6781 ttcaggaatt cgtcgatctc cccgtcgagc acagcctggg cattgcctgc ttcgaggtcg
     6841 gtgcggtggt ccttaaccag cttgtagggg tgcaatgtat atgaacgtat ctgtgatccc
     6901 cactcgatct ttttcttttt gctttcgatc tcggcctggg cttctctctt ctttctgagt
     6961 tctatctcgt agagctgcga tttcagcatc tggatcgctt tttcgcggtt ctgaagctgt
     7021 gagcgggttt cctggcactc cactataatc ccggtcggct cgtgtttcaa ccggaccgcc
     7081 gtctccactt tattcacatt ctgcccgccc ggaccgctgg aacggaaggt gtcccaggtt
     7141 atatcggccg gattgatctc gacctggata ttttcatcca ccatcgggta aacatacact
     7201 gaagcaaaag aagtatggcg ccggttggcc gaatcaaacg gggagaggcg gaccagccgg
     7261 tgcaccccgt tttcgccttt caggtagccg aaggcgtatt cgccgtcaat ttctatcgaa
     7321 accgatttga tccctgccac gtcgccgtcc tgacggtcga gctccttgac tttatggcct
     7381 tgtatctcgg cccatcggat atacatccgc atcagcatgg ccacccagtc ctggctttcg
     7441 gtaccgcctg ccccggagtt gatctggaga atggcgctca gcttgtcttc ttcccggctg
     7501 agcatgttca ggaactccag gttctcgata atttttgtgg tcttttccgc ttgccggtcc
     7561 aggtcttcat ccgtagcttc cccggcttca taaaattcat agatcacaac caggtcgtca
     7621 aaagatgctt tagcctcatc gaaggcatcc gtccatcgtt tgatatcccg gatggaacgt
     7681 aaaacctctt ccgctttttt gggatcgttc cagaaatcct gcgcgtgcgt cagttcttcc
     7741 ttttccgtga tggtttgttt cttcttttcg acgtcaaagg aacctcctca aagcatcaag
     7801 cctttgattc agatttttat aaaattcctg ggtgatcata acgcatattt aagattgtat
     7861 t
//
LOCUS       DAOQXS010000062         7659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_9964_length_7659_cov_3.343503, whole genome shotgun sequence.
ACCESSION   DAOQXS010000062 DAOQXS010000000
VERSION     DAOQXS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9964_length_7659_cov_3.343503"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PKM34_02975"
     CDS             complement(<1..1225)
                     /locus_tag="PKM34_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82577.1"
                     /translation="MIAVVVLIMTAITLYSLLRTPSLQQFAGRLASGFLTERLGHEIR
                     LESLRISDFIYVETRGIEVRDTLDNVILGIENLRVKIDDFSLGRHRLSFERVSIDSGT
                     FAMVYFNDDSLSNLGYFIRKFTRSDTTVTDTTPAPAWHISCSNLLIDRFTYSLKREGV
                     EAGEQDGVNFRDLGIHDISIDMREIAVIGDSISAYIDHLACRENSGLELLNFSGDATV
                     YSKGIIVDGAQISTGRTSLDLDLAFRYDGYDRLSEFLDSVRIEADIRSSLITPGDIGY
                     FVPALAGMDDPVMFSGKVNGPVSDFRATSLSVNLGDFTLFEGDLAINGLPDFDSAFID
                     LSIDQFATTPGEIANLKFPAYDLASVIPDELNTLGYTSLQGNFSGTPHDFRIDAMVRS
                     EAGNATLNGKYTEDRP"
     gene            1275..2303
                     /gene="tsaD"
                     /locus_tag="PKM34_02980"
     CDS             1275..2303
                     /gene="tsaD"
                     /locus_tag="PKM34_02980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNQ82578.1"
                     /translation="MSIYILGIESSCDDTSAAVIRDREMLSNLIANQDVHRAFGGVVP
                     ELASRAHQQNIIPVVQQALDRAKIDKNQLHAVAYTRGPGLLGSLLVGTSFAKAFALSL
                     GIPIIDTDHMEAHILAHFLMEPGVEKESPDFSFFCLTVSGGHTQIVLVKDYFDFTIVG
                     RTIDDAAGEAFDKAAKIMGLPYPGGPLIDKLAKEGDENAFRFPMPSIPGLDYSFSGLK
                     TSFLYFLRDHLKTDPGFIENHKADLAASIQKAIIKTLMKKLLKAASELNIHDIAIAGG
                     VSANSRLRNELLELRETKGWRVFIPKFEYCTDNAAMIAITGYFKYLKGEYGRQDAPVY
                     ARSNMVNI"
     gene            2300..3304
                     /locus_tag="PKM34_02985"
     CDS             2300..3304
                     /locus_tag="PKM34_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNQ82579.1"
                     /translation="MKAIILPEYNPNVIRALKSLHVEETDIPKPAGRNVLVKVAASPC
                     NPSDIAFMRGSYNIRKPVPAVMGFECTGTVVETGDHREAKALLGKRVSCFSQSDENGT
                     WAEYFLTDFNNCLTIREDLDDDQASALCINPFTAFALFSMALDRKANAIIQNGSSGQI
                     GIFIRTLARRNRIPVINLVRKDDHIKVLQDAGEKHVLSMNDPDFESGLAAIAREVRAT
                     IAFDAVGGDISGTIINAMPPGSELVVYGGLSGKPIGMVNPLEVIFKSKSIRGFNLGDW
                     KIEIGSKRFQEISDELQQLMVSRILVTRIQCAFSLEEVHQALEQYIRNMSSGKIIFQP
                     "
     gene            complement(3311..>3752)
                     /locus_tag="PKM34_02990"
     CDS             complement(3311..>3752)
                     /locus_tag="PKM34_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82580.1"
                     /translation="EYIHPYRSEEEVYDEIIVIDGIDVNAINFNLWIELGIMNSEMKN
                     RVLFYSDFLMDKISGTLKQNLMTGKMSFDYSIDTISPGWITEFAYKFGTVSASYFYDY
                     LLNEYIGEKLPDNYPYERYYYHFDPERRLIYPVDESERLTEIKE"
     assembly_gap    3753..3762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3763..4160)
                     /locus_tag="PKM34_02995"
     CDS             complement(<3763..4160)
                     /locus_tag="PKM34_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82581.1"
                     /translation="MRTLRSAILLIFIAALFTGCTLEQRLASSFVKLDPKPQVYLLEP
                     DHIFKYNLKEFELSGSDTMDQAVKDSLLLEKSLFLNTFSDSVVIERLMAGMISGLKRS
                     GLSIIPESDMDSLLGQGGNPFILNLAQFTL"
     gene            complement(4157..5335)
                     /locus_tag="PKM34_03000"
     CDS             complement(4157..5335)
                     /locus_tag="PKM34_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNQ82582.1"
                     /translation="MLDHDIVTFDVVMETKHGTTYNSYFIDAERKALIETVKEGFWDV
                     YLEKLRQITNPEEIDYIILNHTEPDHSGSLAHLLKLAPGATVVGTGNAIRYLKEMIYT
                     DFPHIVVKDGYTLDLGNKTLQFISAPNLHWPDTMYTYLAEEKILFTCDSFGAHYCHEG
                     VFDDKVGDFDEDFRYYFDVILKPFSKFMLKAIEKVNALDINLIAPGHGPVLRSGWKKR
                     VELSEKWAKEHLDLPVSRKVFIPYVSAYHKTGKIAEKIRDGIISAGNLEAEILDIEMT
                     SIGDLEQKVAESAALIVGSTTINQNILLPVYKLFSVINPLRDKGKLAGGFGSYGWSGE
                     SKDLIKNNLENLKLNYFGEGVFVKFTPSPVEEQQAFAYGKAFGEELLAGTNAGTKSEG
                     "
     gene            complement(5419..>7659)
                     /locus_tag="PKM34_03005"
     CDS             complement(5419..>7659)
                     /locus_tag="PKM34_03005"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNQ82583.1"
                     /translation="YDCRNKEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFIELID
                     ASINILKEEEFEIYPDFQTGGLIDVSRYNDGARGGKVRIRAKIKKIDNKTLVIQEIPF
                     GVTTTNLIDSIIQANDKKKIKIRKIDDNTAAEAEILVHLVPGTSPDQTIDALYAFTLC
                     EVPVSPNACVIRDGKPQFLDVREILRISTRNTVGLIRRELEIRLDELREQWHYASLEK
                     IFIEHKIYRKIETCETWEEVIETIDKGLAPFTKKLVRPVTHEDIVRLTEIRIKRISKF
                     DSNKADDSILSLENEMEEVQNHLDHLIDYAILYFEQIRKKYSAGRERKTEIRSFDTIE
                     AAAVAAASQKLYFNREEGFAGTSLKKDEYICDCSDIDDVILFREDGTFLVRKVEGKFF
                     TGKDVVYINVFKKNDERTIYNMVYRDGKKGSCYVKRFAVVGVTRDKEYDLTKGNPGSK
                     VVYFSANPNGEAEMIRIFLRPKPRMKKTTFDFDFSSIAIKGRSSQGNLLSKNPVRQIV
                     KKEHGVSTLGARGIWYDESVKRLNAEERGIFLGAFEGDDKILTLHRSGVYKLYNYDLS
                     THFDEDMLFILKFDPQITVSAVYFDGNTGTYYLKRFAIEPSDKAVGFLNEHKDTRLEE
                     LSIDWLPRLQLAFLEKNGKQRPEEIIDVAGFIGVKSYRAKGRKLSEHAIGQIRWLDPL
                     PYEPSGEEDNGVEQKEEDVVSFEILPAAKPPEEEPEPRKPIPPESGNDEHKPPESGKQ
                     ITLEFF"
BASE COUNT         1919 a         1888 c         1789 g         2053 t
ORIGIN      
        1 tgggccggtc ttcagtgtat ttgccattca atgttgcatt tccggcctcg gaccttacca
       61 tggcgtcgat cctgaaatca tgtggggtcc ccgagaaatt tccctgcaat gaagtatagc
      121 ccaaggtgtt caactcgtcc ggtataactg atgccaggtc gtacgccggg aattttaaat
      181 tggcaatttc tcccggtgtc gtagcaaact gatcgatgga aagatctata aaggccgaat
      241 cgaaatcagg taatccatta atagccaaat cgccttcaaa caaagtaaaa tcgccgaggt
      301 taaccgacag gcttgttgca cggaaatcgc tgacggggcc attcacttta cccgaaaaca
      361 tcaccgggtc atccataccg gccagtgccg gaacaaaata cccaatatct ccaggagtaa
      421 tcagtgagct tctgatatca gcctcgattc gtacactgtc aagaaattcg gataaacggt
      481 cataaccgtc gtaccggaaa gccaggtcaa ggtcgaggct ggtgcggccg gtagagatct
      541 gcgccccgtc gacaattatg cctttagaat aaactgttgc atctccactg aaattcagca
      601 gttccagacc agagttctcc cggcaggcca ggtgatcgat ataggccgag atggagtcgc
      661 caatcaccgc tatctctctc atatcgatgg aaatatcatg aatacccagg tctctgaagt
      721 tcacaccatc ctgctctcca gcctccactc cttcccgctt cagggaatag gtgaaccggt
      781 cgatcagcag gttgctgcag gaaatatgcc aggcaggtgc cggcgtagtg tcggtaactg
      841 ttgtgtcaga ccgggtaaac tttctgataa aatagccgag gttcgacagg ctgtcatcat
      901 taaaataaac catagcgaaa gttccactgt caatcgacac cctttcaaac gagagccggt
      961 ggcggccgag ggaaaaatca tcaatcttaa cccggaggtt ttcaatacca aggattacat
     1021 tgtcaagggt atccctgact tcgatacccc gggtctcaac atagataaag tcactgatcc
     1081 tcagactctc cagccggatt tcatgcccaa gtcgctctgt aagaaatcct gatgcgagtc
     1141 gcccggcaaa ttgctggagg gaaggggtac gcagcaatga atacaaggtg atggctgtca
     1201 tgataagcac gacgacagct atcacacacg ccttaaggat atttttaata attttgcccg
     1261 taagaataag atttatgagc atttacatcc ttggtattga atcctcctgc gacgacactt
     1321 cggcggcggt gatccgcgac cgcgaaatgt tatccaacct gattgccaac caggatgtgc
     1381 accgggcatt cggcggcgtt gttcccgaac ttgcctcgag agcccaccag cagaacatca
     1441 ttcctgtggt tcaacaagcc ttggataggg caaagataga taaaaatcaa ttgcatgccg
     1501 tagcctatac ccgggggccg ggattgctcg gatctctgct ggttggcacc tcttttgcca
     1561 aagcatttgc cctttccctt gggatcccga tcatcgatac cgatcacatg gaagcgcaca
     1621 ttctggccca tttcctgatg gaaccagggg tggaaaaaga atccccggat ttctcttttt
     1681 tttgtctgac ggtatcggga gggcacaccc agatcgttct cgtaaaggat tatttcgatt
     1741 tcacgattgt gggaaggaca atcgatgacg cagccggaga ggcgttcgac aaagcggcca
     1801 agatcatggg cctcccctat cccggcgggc cgttgatcga taaactggca aaggaggggg
     1861 atgaaaatgc atttcgcttt cctatgcctt ctattcccgg tctcgactac agtttcagcg
     1921 gattaaagac ctccttcctc tattttctcc gcgaccatct taaaacagac ccggggttta
     1981 tcgaaaatca caaagccgat ctggcagctt ccatccagaa agccattatc aaaactttga
     2041 tgaaaaaact gctcaaggca gcctcggaat taaacatcca tgacattgcc attgcagggg
     2101 gtgtttcagc caactccagg ctcagaaacg aattgctgga gctgagagaa acgaaaggct
     2161 ggcgagtctt cattccaaaa tttgaatatt gtaccgacaa tgccgccatg atcgccatca
     2221 ccggttattt taaatatctc aaaggagaat atggcaggca ggatgcaccg gtatatgcca
     2281 gaagcaatat ggtgaacata tgaaagcaat tattcttcct gaatacaacc ccaacgtgat
     2341 ccgggcatta aaaagtctcc atgtggaaga gaccgatatc ccaaagcctg cgggccggaa
     2401 tgtcctggta aaagtcgctg cctcgccctg caatccttcc gatattgcct ttatgcgcgg
     2461 cagttacaat atacgcaaac cggtgcccgc ggtaatgggt tttgaatgca ccggaaccgt
     2521 ggttgagacc ggagatcatc gcgaagcaaa ggcgctgctt ggaaaaaggg tgagctgctt
     2581 ttcccagtcg gatgagaatg gaacctgggc ggagtacttt ctcacggatt ttaataactg
     2641 cctgactatc agagaagatt tggatgacga tcaggcttcc gcgctatgta tcaacccgtt
     2701 taccgcattc gcgttgttca gcatggctct cgacagaaag gctaatgcca ttattcagaa
     2761 tggctcttca gggcaaatcg gaatctttat ccgaaccctg gcccggcgta accggatccc
     2821 ggtcattaac ctggtgcgta aagatgatca tatcaaggtt ttgcaggatg ccggggaaaa
     2881 gcatgtgctc agcatgaacg atcctgattt tgaatccggt ctggctgcaa tagcccgtga
     2941 agtaagggcc accattgcat ttgatgcggt cgggggggat atctcaggta ccataatcaa
     3001 cgccatgcca ccaggctccg aactggttgt ttatggcgga ttatccggaa aaccgatagg
     3061 aatggtcaat cctcttgaag ttatattcaa atcaaaatcc atcaggggat ttaacctggg
     3121 cgactggaag atagaaatcg gcagcaaaag attccaggag atcagtgacg agcttcagca
     3181 gctgatggta tcacggatac ttgtaacccg tatccagtgc gccttctcac tcgaggaggt
     3241 tcaccaggca ctcgaacagt acatccgcaa catgtcgtcg ggaaaaatca ttttccagcc
     3301 ctgatgcatc tcattccttg atttcggtaa gtcgttcgct ctcatctacc gggtaaatca
     3361 gcctccgttc cggatcaaaa tgatagtagt acctttcgta gggatagtta tcgggaagtt
     3421 tctcgcctat atattcattc aacaggtaat cataaaaata agatgccgag acggttccga
     3481 atttataagc gaattccgtt atccacccgg gcgagatcgt atcgatggag tagtcaaagc
     3541 tcatcttgcc ggtcatgagg ttttgtttga gggttccgga aattttatcc atcaggaaat
     3601 cagaataaaa aaggaccctg ttcttcattt ctgagttcat aattcccagt tctatccata
     3661 agttgaaatt gatcgcattt acatcaattc catcaatcac aataatttca tcatatacct
     3721 cttcttcgct cctgtatgga tgaatatatt ctnnnnnnnn nntccaaagt gaactgggca
     3781 aggttgagga taaacgggtt ccccccctgg ccaagcaatg aatccatatc gctttcagga
     3841 atgatgctca agcccgacct cttaagaccg ctgatcattc cggccatcaa tctttcgatg
     3901 accactgaat cactgaaagt attcaaaaac aaactctttt caagaaggag gctgtctttg
     3961 acagcctgat ccatcgtgtc ggagcccgaa agttcaaact ctttcaggtt atatttaaag
     4021 atgtggtcag gctccagaag gtatacctgt ggtttcggat caagctttac aaaagaggaa
     4081 gccagtcttt gttcgagggt gcaaccggtg aacaaagctg caatgaagat caatagtatg
     4141 gctgatctta gcgttctcaa ccttcagatt ttgtgccggc atttgtgccc gcgagcaatt
     4201 cttcaccgaa agcttttcca taagcgaagg cctgttgttc ttcaaccggg ctcggggtga
     4261 atttcacaaa gactccttcg ccgaaataat tcagcttcag gttttccagg ttattcttaa
     4321 tcaggtcttt gctctctccc gaccagccgt aagagccaaa acctccagcg agcttgcctt
     4381 tgtcgcgaag cgggttgata accgagaaaa gcttataaac cggaagcagg atattctggt
     4441 taatggttgt gctcccaaca atgagggcgg cactctccgc aactttttgt tccaggtcgc
     4501 cgatactggt catttcgata tcgaggatct ctgcttcgag gtttcctgcc gaaatgatcc
     4561 catctcttat cttttcagca atcttaccgg ttttgtgata agcagataca taagggataa
     4621 agactttgcg gctaacggga agatccaggt gttctttcgc ccatttttct gaaagttcca
     4681 ctcgtttttt ccatcctgac ctcaaaaccg gtccgtgacc cggggctatc aggtttatat
     4741 ccagggcatt gactttctca atagccttca gcataaattt gctgaacggt ttcaggatga
     4801 catcaaaata atacctgaaa tcttcatcaa agtctcccac tttatcatca aaaacacctt
     4861 cgtggcaata atgggcgcca aatgaatcgc aggtaaataa aatcttttcc tccgccaggt
     4921 aggtgtacat ggtatcgggc cagtgcaggt tgggggcgct gataaactgc agggttttat
     4981 tgcccaggtc gagtgtataa ccgtctttta caacaatatg cgggaaatcg gtatagatca
     5041 tctccttcag atagcggata gcattcccgg ttccgaccac tgtggcgccg ggggccagtt
     5101 tgagcagatg cgccaggctt cctgaatggt caggctcggt gtgattgaga atgatgtaat
     5161 caatttcttc ggggttagtt atctgcctga gcttttcgag ataaacatcc cagaatccct
     5221 ctttgactgt ctcgataagg gcttttcttt cagcatcgat aaaataagaa ttataggtcg
     5281 taccgtgctt tgtctccata acaacgtcga aagttacgat atcgtgatca agcacgccgc
     5341 accatttcac tgaaggacta acatcatgta ctttgttgtc gggttgcata gcattatttt
     5401 acttttcctc tgttatgatt aaaaaaattc cagggtgatc tgcttaccgg attcgggagg
     5461 tttgtgttca tcattcccgg attcgggcgg aattggcttc cggggttcag gttcttcctc
     5521 cggaggcttc gcagccggaa ggatttcaaa ggaaacgaca tcctcttcct tttgctctac
     5581 gccattatcc tcttccccgg acggttcata gggcaacgga tcgagccagc gtatctgccc
     5641 gatggcatgt tccgacagct tcctgccctt ggcacggtaa ctcttcaccc ctatgaaacc
     5701 ggccacatca atgatttctt ccggtcgttg ttttccgttc ttttcaagga aagctaactg
     5761 caaccggggc agccagtcga tactcagctc ttccagccgg gtgtctttgt gttcattcag
     5821 aaaaccgacc gctttatccg aaggctcaat agcaaacctt ttcaaatagt atgtgccggt
     5881 attgccgtcg aaataaacgg cggatacggt aatctgcggg tcgaatttca gaatgaagag
     5941 catatcctca tcaaagtgag tcgacaggtc gtaattataa agcttataga cgcctgaccg
     6001 atgaagagtc aggatcttat cgtcgccttc aaaagcaccg agaaagattc ccctctcttc
     6061 agcattgagc cgtttgaccg attcgtcata ccatatgccc cgggcgccga gcgtcgatac
     6121 cccatgttct tttttaacta tctggcgtac cgggtttttg gataacaggt ttccctgcga
     6181 acttctgcct tttatggcaa tggaactgaa gtcaaaatcg aaagttgttt ttttcatcct
     6241 gggtttcggg cgcaggaaaa tcctgatcat ctcggcttcc ccgtttggat tggcactaaa
     6301 gtaaacaacc tttgatccgg ggtttccctt tgtcagatcg tattctttat cccgcgtgac
     6361 gccaaccacg gcgaaccgtt tcacataaca cgaccccttt ttgccatccc ggtagaccat
     6421 attgtatatc gtccgttcat cattcttttt aaagacattt atgtaaacga catcttttcc
     6481 cgtaaaaaac ttcccttcca ctttcctgac caggaaggtt ccgtcttccc ggaagaggat
     6541 cacatcgtcg atatcggagc aatcgcagat atattcgtct tttttcaggg atgtgccggc
     6601 aaatccctct tcacggttga aatagagttt ctggctggct gctgccacgg ccgccgcttc
     6661 gatcgtatcg aaactcctga tctctgtttt acgctcacgg ccggcgctgt actttttcct
     6721 gatctgttcg aaatagagaa tggcataatc gataagatga tcgaggtggt tttgaacctc
     6781 ttccatctca ttctccagcg aaaggataga atcgtcggcc ttgttggaat caaattttga
     6841 gatccttttg atcctgattt ctgtcagcct gacaatatct tcatgcgtta cgggccttac
     6901 cagctttttc gtaaacggcg ccagcccttt gtcgatggtt tcaatcacct cttcccaggt
     6961 ttcacaggtt tcgatcttcc ggtagatttt atgttcgata aagatcttct ccaggctggc
     7021 ataatgccat tgttcacgca attcatccag cctgatctcc agttcgcgcc ggatcagtcc
     7081 gacggtattc cgggtggaaa tacggaggat ctccctgaca tccagaaatt gcggttttcc
     7141 gtcgcggatc acgcacgcat tgggcgagac aggcacctca cagagggtga aagcgtaaag
     7201 ggcgtcgatg gtctgatcgg gtgaggtacc gggaacaaga tggacgagga tttcagcttc
     7261 ggcagccgta ttatcgtcga ttttcctgat cttgatcttc ttcttgtcgt tggcctggat
     7321 gatggaatcg atcaggttgg tggttgtaac gccgaaggga atttcctgta tgaccagcgt
     7381 tttgttgtcg atctttttga tcttcgccct gatcctcacc ttgcctcccc gggcgccgtc
     7441 gttgtacctc gaaacatcga tcagtccgcc ggtctgaaaa tcgggataga tttcgaattc
     7501 ttcttctttt aagatgttga tggaagcgtc gatcagctca ataaaattgt ggggaaggat
     7561 ctttgatgca agtccgacgg cgattccttc cactccctgg gccagcagca gcgggaattt
     7621 gaccggcagg gtgaccggct ctttgttccg gcagtcgta
//
LOCUS       DAOQXS010000063         7636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10003_length_7636_cov_3.898958, whole genome shotgun sequence.
ACCESSION   DAOQXS010000063 DAOQXS010000000
VERSION     DAOQXS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10003_length_7636_cov_3.898958"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..2382)
                     /locus_tag="PKM34_03010"
     CDS             complement(109..2382)
                     /locus_tag="PKM34_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNQ82584.1"
                     /translation="MQSGRNKTSGKKGSNTYLRRFWIIYVLSMVLIVLFFAGIANNLF
                     GPLPSFEELENPKTSLATEIYSSDKKLIGTYYIENRSNAEYIELPQHLVQALLATEDI
                     RFEKHAGVDLKALFRVAFGVITGNSKGGGSTLTQQLAKNLFPRERDLSKPQLVMRKFK
                     EWITAIRLERNYSKQEILAMYLNTVDFGSQSWGIKIASKTFFSKPVDSLNLQESALLI
                     GVINAPTWYSPVRNPERSFERRNLVLRQMERYKFITKEVCDSVSNIPLDMTAFGLRGH
                     STGLATYFREILRMELKDWCSKHYKSDGTPYDLYKDGLKIYTTIDSRLQRYAEEAMTE
                     HLKNDLQPSFFAHWRRHVNAPFVFEPDVAAVEIKNLLEQSMKRSDRYRQLKEEGVPLD
                     SIRMIFNTPVKMRVFSWKGPVDTVMTPLDSIRYSKYFLQAGLMSMDPHSGYVLAYVGG
                     IDYRYFQYDHVKLSKRQVGSTFKPFLYSLAMQEGEFTPCSKIANVQYTVDLPDGTVWA
                     PKNTNKKRRGEEVTLKWALANSNNWISAYLIKRYSPQSVITMARKMGVTADLDPVPSL
                     ALGSSDISLYEMTGAFNTFANKGVHIKPIMIVRIEDKYGNVIESFVPEKQEAMSEETA
                     YLMLELMKGVVQSGTGIRLRTKYNFTNPIAGKTGTTQNQSDGWFIGITPELTTGVWVG
                     AEDRSVHFRTLGLGAGANTALPIWALYMKKAYADHSLRISQGDFERPSQPLSVSLDCD
                     KYEKEQDNRLEDEIIEF"
     gene            complement(2398..2910)
                     /locus_tag="PKM34_03015"
     CDS             complement(2398..2910)
                     /locus_tag="PKM34_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNQ82585.1"
                     /translation="MTAGLLTGIKETARAFSRMMMPAAVFSLLVVTQGCGRPPLFDQN
                     KSFENHVWNLDDIIRFDVNVTDTVHIHKYFLNVRHSTDYRYANLYVFINSTFPDGRQA
                     RDTVECILAEPSGKWLGKGISTVRDNQILLRRGLRFPKTGNYIFEIEQAMREEELHGI
                     MDIGIRIERE"
     gene            complement(2913..4028)
                     /gene="ricT"
                     /locus_tag="PKM34_03020"
     CDS             complement(2913..4028)
                     /gene="ricT"
                     /locus_tag="PKM34_03020"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNQ82586.1"
                     /translation="MEEDKKHSCGDAFLSRGCVCFPKMYDNNKHTFKPGCSKLDSSDW
                     LKDIDLPPGQKPFDCVEIRFKNSRKEFFRINTDYDIRQGDIVAVESSPGHDIGIVTLA
                     GEACRIQMKKKNVDPTLDTIKKVYRKARATDIEKWISCVEIEDDTMFKSRQAATDLDL
                     DMKISDVEYQGDGTKAIFYYTADERIDFRQLIKTLAEMFRVRIEMRQIGVRQEASRLG
                     GIGSCGRELCCSTWLSRFRSVTTQAARVQQLALNPQKLAGQCSKLKCCLNYEYDVYVD
                     TAREFPDTRIFLKTKKGICIHQKTEVFRRIMWYSYEGAPETLIPLPLDVVKEIIGMNN
                     KGKIPDKIEDFIQNKETKTLPQDGSDQFELHRFDKKK"
     gene            complement(4063..5241)
                     /locus_tag="PKM34_03025"
     CDS             complement(4063..5241)
                     /locus_tag="PKM34_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNQ82587.1"
                     /translation="MLFRDITGQEEIKRRLIQTVKDNRVSHAQLFHGPEGSRKLAMAI
                     AYARFINCPNRSFGTKDGTMGDACGVCPSCIKFSKLIHPDLHFIFPVATTKEIEKDPI
                     SKDFLKTWREILLESNFRLSLNDWYKAANFEKKQGIINADDCSEILKTLSYKSYESEF
                     KVMIIWMADRLYHSAAPKILKILEEPPDKSLFILITENPEKIINTILSRTQAIKFPRL
                     ADDDLVWELTQKYGYPPGEAARVIPVADGNLTRAVKILKNDEDESFYTKTFVEWMRLC
                     YKHEMKHTMDFVADIAKIGREKQKNFLLYAERVIRGAFLSNFDHPALGRLNDEEKEFL
                     IKFGKFINHSNIVKFSEELEKAQVHIDRNANPSILFMDLSMTVTILLLTGQKAAASSQ
                     "
     gene            complement(5243..6019)
                     /locus_tag="PKM34_03030"
     CDS             complement(5243..6019)
                     /locus_tag="PKM34_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNQ82588.1"
                     /translation="MDFTLKIWRQENAKAKGRFVTYQVTDISPDCSFLEMLDILNESL
                     VDKFEMPIGFEHDCREGICGTCALYINGRPHGPDKGITVCQMHMRRFKSGTTIVIEPW
                     RSRAFPVLRDLMIDRSAFDKIIQAGGYISIATGNAPDAHALPVEKLNSDLAMDAAACI
                     GCGACVAACKNASAMLFVSAKVSQLALLPQGRPEARKRVQAMVAKMDELGFGNCTNTY
                     ACEAECPKLIKITNIARMNREFFAAKVCGAKEVATTGGAG"
     gene            complement(6021..>7636)
                     /locus_tag="PKM34_03035"
     CDS             complement(6021..>7636)
                     /locus_tag="PKM34_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNQ82589.1"
                     /translation="REANVYRLAEVSNGIIDQCVAQGVPFAREYGGLLDNRSFGGALV
                     SRTFYARGQTGQQLLLGAYGALSKEIEKGSVTMYTRRDMLDLVVIDGRARGIIVRNNV
                     TGEIERHSGHAVILATGGYGNVFFLSTMAMHSNGSAAWSAYKRGAFFGNPCFTQIHPT
                     CIPVHGDYQSKLTLMSESLRNDGRIWVPKKKEDAEKIRKGELKPGDIPEEDRDYYLER
                     RYPAYGNLSPRDISSRAAKERCDAGYGVGSTGLAVYLDFADAIQRLGKGVIEARYGNL
                     FQMYEKIVDQNPYETPMMIYPAVHYTMGGLWVDYELQTTIPGLFVGGEANFSDHGANR
                     LGASALMQGLSDGYFVLPYTVQNYLADQFKVPRFSTDLPEFLAAEKEVKDNINRLLGI
                     KGKKSVDTFHRELGRIMWENVGMGRNKEGLEKALKLIPELREEFWKDVRIPGTNDELN
                     PELQKATRLADYLELGELMALDALNREESCGGHFREEYQTPDGEALRNDKDFAYVAAW
                     EYKGTENKAELHKEPLNYEFVELKQRNYK"
BASE COUNT         1612 a         2028 c         1937 g         2059 t
ORIGIN      
        1 tattttatgt atcccgagag ttctaaacag tttattcatt tcaaatgatc gtgtatttgg
       61 ccaattcagg gtatttacac tccggaaaaa tcagatccgg tggaagtatc agaattcaat
      121 tatttcatct tcaagccggt tgtcctgctc cttttcatat ttatcgcaat caagggaaac
      181 ggataagggt tgcgaaggcc tctcgaaatc accctggctg atccggagcg aatgatcggc
      241 ataggctttt ttcatataca gcgcccagat cggcagcgct gtattggcgc ctgctcccag
      301 tccaagcgtc cggaagtgga cgctgcggtc ttccgccccg acccaaaccc cggtggtgag
      361 ttccggcgta atgccgatga accacccgtc tgactggttc tgtgtcgtac cggtttttcc
      421 cgcaatcgga ttggtgaaat tgtatttcgt ccgcaaccgg atacccgtac cgctctgtac
      481 aacccctttc atcagctcca gcatcaggta ggcggtctct tcgctcatcg cttcctgttt
      541 ctcgggcacg aacgactcga tcacattgcc gtatttgtct tcgatcctga cgatcatgat
      601 gggtttgatg tgaacgccct tgttggcgaa ggtgttgaag gcgccggtca tttcgtaaag
      661 tgagatatca gacgaaccta aagccagcga cggaaccggg tcgagatcgg cggtaacgcc
      721 catctttctg gccattgtta tgaccgattg aggggaatac cgtttaataa gataggctga
      781 tatccagttg tttgagttgg caagtgccca cttaagagtc acctcttcgc ctcttctctt
      841 tttattggtg tttttaggcg cccaaaccgt gccatccggc agatctaccg tgtactgcac
      901 gttggcgatc ttgctgcacg gcgtaaactc accctcctgc atcgccaggg aatacaggaa
      961 gggcttgaac gtcgacccga cctgcctttt gctcagtttg acatgatcgt actgaaaata
     1021 acggtagtcg attccgccga catacgccag gacgtaaccg gaatggggat ccatcgacat
     1081 caggccggct tgcaggaagt atttgctgta acggatcgag tccaatggcg tcataacggt
     1141 gtcaaccggc cctttccacg aaaagacacg cattttgacc ggtgtgttga agatcatccg
     1201 gatggaatcg agcggtaccc cttcctcttt gagctgacgg taacggtctg accgcttcat
     1261 cgactgttcc agcaagttct tgatctccac agccgccacg tcaggctcaa atacgaatgg
     1321 tgcattgaca tgccggcgcc agtgggcaaa gaaagagggt tgcagatcgt ttttcaagtg
     1381 ttcggtcatc gcttcctcgg cgtaacgctg caaacgtgag tcgatcgtcg tatagatctt
     1441 cagtccgtct ttatacagat catatggagt cccgtctgat ttataatgct tgctgcacca
     1501 gtctttaagt tccatgcgca ggatttccct gaagtaggtg gccagccctg ttgagtggcc
     1561 ccggagaccg aatgccgtca tgtcaagggg gatgttggag accgaatcgc aaacttcttt
     1621 agtgataaat ttatagcgct ccatctgcct cagtaccaga tttctgcgtt cgaacgaccg
     1681 ttccgggttc cgcaccgggc tgtaccaggt cggcgcattg atcacaccga tcagcagggc
     1741 cgattcctgg aggttcaaag aatcgaccgg tttgctgaag aaggtctttg aagcgatttt
     1801 tattccccac gactggcttc cgaagtcgac cgtgttgaga tacatcgcca ggatttcctg
     1861 tttggaataa tttcgttcca gccggatagc ggtgatccat tccttgaatt tacgcatgac
     1921 cagttggggt ttcgacaggt cgcgttcccg cgggaataag ttcttggcta attgctgagt
     1981 cagtgtactt ccccctcctt tgctgttccc ggtgatcacc ccgaaagcca cgcggaacaa
     2041 agctttcagg tcgacgccgg cgtgcttctc gaaacggata tcctccgtgg ccagcagggc
     2101 ctgaaccagg tgctgcggca gttcaatgta ttcggcgttc gaccggtttt cgatataata
     2161 cgttccgatc agtttcttgt cagaggaata tatctcggta gccaggctgg ttttaggatt
     2221 ctccaattct tcgaaagagg gcagggggcc gaagagattg ttggcgatcc ccgcaaaaaa
     2281 aagtacaatc agtaccattg acagtacata gatgatccag aaccttctca ggtaagtgtt
     2341 actgcctttt ttgcccgatg tcttgttcct tccggattgc atgtcattct ggttaagtta
     2401 ttctctttca atacggatgc cgatgtccat gatcccatgt agctcctctt ccctcatcgc
     2461 ctgttctatc tcgaagatat agtttccggt ttttggaaaa cgcagcccac gccgaagcag
     2521 gatctggttg tcgcggacgg tgctgatccc tttcccgagc catttgcccg atggttcggc
     2581 gaggatgcat tcgacagtgt cgcgggcttg cctgccatcc ggaaaagtac tgttgatgaa
     2641 cacatacagg ttggcgtaac gataatcggt cgaatgtctc acattcagaa agtatttatg
     2701 gatatgtacc gtatcggtta catttacatc gaaccggata atatcatcca aattccaaac
     2761 gtgattttca aaggatttat tttgatcaaa caatggcggt cggccacaac cctgcgtgac
     2821 gaccagcaat gagaataccg ccgccggcat catcatcctg ctgaaagccc ttgccgtttc
     2881 ttttatgccg gtgagcaggc ctgcagtcat cttcatttct ttttatcaaa tcggtgaagt
     2941 tcgaactggt cggagccatc ctgaggcagt gttttggtct ccttattctg aataaagtct
     3001 tcaattttat ccggaatttt tcctttatta ttcatgccga tgatctcctt tacgacatcc
     3061 agcggcagcg ggatcagggt ttcaggggcg ccttcgtatg aataccacat gatccttctg
     3121 aatacttccg ttttttggtg gatacaaatg cctttttttg ttttcaggaa gattcgggta
     3181 tcggggaatt cccttgccgt atcgacataa acatcgtatt cataattcag gcagcatttc
     3241 agcttgctgc actggccggc cagtttctgc ggattcaggg caagttgctg tacacgtgct
     3301 gcctgtgtcg tcaccgacct gaagcgtgaa agccaggtgg aacagcacaa ttcccggccg
     3361 catgagccta tgcctcccag gcggctggcc tcctgcctga cgccgatctg gcgcatctcg
     3421 atccttaccc tgaacatctc cgccagggtc ttgatcagtt gcctgaaatc aatccgctca
     3481 tcggcagtgt aataaaaaat agctttcgtg ccatcaccct ggtattccac atcgctgatc
     3541 ttcatatcca ggtccaggtc ggtggccgcc tgccttgatt tgaacatcgt atcgtcttcg
     3601 atttccacac aggatatcca tttttcgata tctgttgccc tggctttgcg ataaactttt
     3661 ttgatggtat cgagcgtggg atcgacattc tttttcttca tctggatgcg gcatgcctct
     3721 ccggcgaggg taactatgcc gatgtcgtgc cccggtgatg actccaccgc tacaatatcc
     3781 ccctgccgga tgtcgtaatc ggtattgatc ctgaagaatt ccttccggct gtttttgaat
     3841 cggatctcca cacaatcgaa cggtttctgg ccgggtggca ggtcgatgtc cttcagccag
     3901 tcggaactgt cgagcttcga acagcccggc ttaaaggtat gcttgttgtt gtcgtacatt
     3961 ttcggaaagc aaacgcatcc tctcgacaaa aacgcatccc cgcagctatg ttttttatcc
     4021 tcttccatca ggatcaattt tcgacaaaaa tacaaataat gatcattggc tgcttgctgc
     4081 cgctttctgg ccggtcagca gtaaaatcgt cacggtcatc gacaggtcca tgaataatat
     4141 cgacggattg gcattccggt cgatgtggac ctgggctttt tcaagctctt cgctgaattt
     4201 cacaatattc gaatgattga tgaatttccc gaatttgatc aggaattctt tctcctcatc
     4261 attcagccgg cccagggccg gatggtcgaa gttcgaaaga aaagcccccc ggataaccct
     4321 ttcggcatac aataaaaaat tcttttgttt ttcgcgtccg atttttgcga tgtcggctac
     4381 aaaatccatg gtgtgcttca tttcatgttt atagcatagc cgcatccatt caacaaaagt
     4441 cttagtgtaa aatgactcat cttcgtcatt tttgagaatt ttaaccgcac gcgtgagatt
     4501 tccgtcggct accgggatga cccgcgccgc ttctccgggt ggataaccat atttttgcgt
     4561 cagttcccac accaggtcat catcggccag ccgtgggaat ttaatggcct gggtcctcga
     4621 aaggatcgta ttgatgattt tttccggatt ttccgtgatg aggatgaaga gcgacttatc
     4681 gggcggttcc tccagaattt tcaggatttt aggggcggcg gaatggtaca gccggtcggc
     4741 catccagatg atcatgactt tgaattccga ctcgtagctc ttgtaactga gtgttttcaa
     4801 aatttcactg caatcatcgg cattgatgat cccctgtttt ttttcaaaat tggcggcttt
     4861 gtaccaatcg ttcaggctga gccggaagtt tgattccagt agtatttccc tccaggtctt
     4921 caggaagtct ttgctgatag gatctttttc aatctctttt gtcgttgcta ccggaaatat
     4981 aaaatgcaga tcggggtgga tgagtttgga gaatttaata caggatgggc atactccgca
     5041 cgcatcgccc atcgtaccat ccttcgttcc gaatgaccgg ttggggcaat tgataaaccg
     5101 ggcataagcg atagccatgg ccagtttacg cgacccttcc gggccgtgga acaactgggc
     5161 atggctgacc ctgttatctt taacggtctg aataagccgg cgcttgatct cttcctggcc
     5221 ggtaatatct ctgaaaagca tcctatcctg cccctccggt cgtggccacc tctttcgcgc
     5281 cgcaaacctt tgctgcaaaa aattcgcggt tcatccgggc aatattggtt atcttgatca
     5341 gtttcgggca ttccgcctca caggcatacg tgttggtgca gttgccgaat cccagctcgt
     5401 ccatcttggc caccatggcc tggacgcgct tccgggcttc cggcctgccc tgtggcagca
     5461 aggcaagctg cgacactttg gccgacacaa agagcatggc cgaagcattc ttgcacgcag
     5521 caacacaagc gccgcaaccg atgcaggctg ccgcgtccat ggccaggtcc gaattcagct
     5581 tttcgaccgg aagtgcatgt gcatccggtg cattgcccgt ggcgatggaa atatagcccc
     5641 cggcctggat gatcttatcg aatgccgacc ggtcgatcat caggtcgcgc agcaccggga
     5701 aggctctcga ccgccagggc tcgatcacaa tagtcgtccc gctcttgaac cggcgcatat
     5761 gcatctggca aactgtaatg cctttgtcgg ggccgtgtgg ccggccgttg atatataatg
     5821 cacaggtgcc gcaaatgcct tccctgcaat catgctcgaa tccgatcggc atctcgaact
     5881 tgtcaacgag gctttcattc agaatatcaa gcatctcaag gaaagaacaa tcgggagaaa
     5941 tatcggttac ctggtatgtc acaaagcggc ctttcgcttt ggcgttttcc tgccgccata
     6001 tttttaacgt gaagtccata ttatttgtag tttctttgtt tcagttcaac aaattcgtaa
     6061 ttgagcggtt ccttgtgcaa ttccgcttta ttttccgtgc ctttgtattc ccaggccgct
     6121 acgtaggcaa aatccttgtc gttgcgaagc gcttcgccgt caggggtctg gtattcctca
     6181 cggaaatggc cgccgcagct ctcctcgcgg ttcagggcgt cgagcgccat cagttcgccc
     6241 agttccaggt aatcggccag ccgggtggct ttctgcagtt cgggattgag ctcatcgttc
     6301 gtaccgggta tacggacatc tttccagaac tcttctctca gctccgggat cagtttcagg
     6361 gctttttcca atccttcctt gtttcttccc atcccgacat tctcccacat gatccggccc
     6421 agctctcgat ggaaagtatc taccgatttc ttccctttga tgcccagcag gcggtttata
     6481 ttgtctttca cctctttttc cgctgccagg aattccggca gatcggttga gaaccggggt
     6541 accttgaact ggtcggccag gtaattctgg accgtgtagg gcagtacgaa gtagccgtcg
     6601 ctcaggccct gcatcagggc cgaagcgccc agcctattgg cgccgtggtc ggagaagttg
     6661 gcttcgccgc cgacgaacag gccgggaatc gtggtttgca gttcataatc gacccacagg
     6721 ccacccatgg tgtaatgcac cgccgggtag atcatcattg gggtttcata ggggttctgg
     6781 tcgacgatct tttcgtacat ctggaacagg ttgccgtagc gggcttcgat cacccctttg
     6841 cccagccgct ggatggcgtc ggcaaaatcg aggtaaacgg ccagaccggt cgagccgacg
     6901 ccataaccgg catcgcaccg ttccttggca gcccgcgagg agatatccct ggggctgagg
     6961 ttgccgtaag ccggatatct tctttcgagg taataatcgc ggtcttcttc cggaatgtcg
     7021 cccggtttca attcaccctt gcggatcttt tcggcgtctt ctttcttttt cggcacccag
     7081 atgcggccgt cgttgcggag gctttcgctc atcagggtca gtttcgactg gtaatcgcca
     7141 tggaccggga tgcaggtcgg gtggatctgt gtgaagcagg gattgccgaa gaaggcgccc
     7201 cgtttgtacg ccgaccaggc cgcactgccg ttggagtgca tggccattgt agagaggaag
     7261 aagacattgc cgtatccgcc ggtggccagg atcacggcat ggcccgaatg acgctcgatc
     7321 tcgccggtaa cattgttgcg gacgatgatg ccgcgcgccc tgccgtcgat cacgaccagg
     7381 tcgagcatat cacgacgcgt gtacatggtc accgagcctt tctcgatctc tttgctgagg
     7441 gcgccgtatg cgccgagcag tagctgctgc ccggtctggc cgcgggcgta gaaggtgcgg
     7501 gaaacgagcg cgccgccgaa ggagcggttg tcgagtaacc cgccatactc gcgggcgaac
     7561 ggaacgccct gggctacgca ctggtcgatg ataccgttac tgacctcggc aagccggtac
     7621 acgttggcct ccctcg
//
LOCUS       DAOQXS010000064         7549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10156_length_7549_cov_4.127168, whole genome shotgun sequence.
ACCESSION   DAOQXS010000064 DAOQXS010000000
VERSION     DAOQXS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10156_length_7549_cov_4.127168"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKM34_03040"
     CDS             complement(<1..1013)
                     /locus_tag="PKM34_03040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ82590.1"
                     /translation="MKISYNWLRRYVDLDLEPEELARKLTSSGLEVEDYEKTETVKGG
                     LKGVCVGLVVECEKHPNADKLSLTKVDTGIGRLLSIVCGAPNVRAGQKVPVATVGTIL
                     FQGDKSFEIKEAKIRGEFSEGMICAEDELGLGTSHEGIMVLDPEAEIGMPAAEYFHVS
                     EDYIFEIGLTPNRTDAMSHLGVARDLVAVLNREAGERKYALKWPSVEEFAVDNHNRII
                     PVTVADATACPRYSGVTISGIKVAESPEWLKNLLNAAGIRPISNVVDVTNFVMLELGQ
                     PLHAFDASRIRGGKVVVRKALNDELFVTLDETERKLSGDDLMICNESEGMCIAGIFGG
                     AGSG"
     gene            complement(1073..3178)
                     /gene="recG"
                     /locus_tag="PKM34_03045"
     CDS             complement(1073..3178)
                     /gene="recG"
                     /locus_tag="PKM34_03045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNQ82591.1"
                     /translation="MTRDFIQTPVEFLKGVGPKRAELLRSELNIHHFGDLLNYYPFRY
                     IDRSRIFKISEIEPDTAYVQIRGTVTNIRIIGEKRARRMSALLRDDTGEIELVWFQGI
                     KWIEGTILPNVEYIVFGKPTIFNRRYNIAHPEIERAEDFLRTVPQTLHGIYSTTEKLK
                     NRGLNNKNLVKLIRELIIQAEGKLHETLPDEIIRKLSLLGREEALRNIHFPLNQQLLQ
                     KAQARLKFEELFFIQLGLLKDKMIRQQRISGFVFSRVADKVNRFYSEKIPFNLTEAQK
                     KVIREIRKDLGAGKQMNRLLQGDVGSGKTLVALMAMLIAIDNGFQSCLMAPTEILASQ
                     HLATLTRMTDGLGIGIGLLTGSTPAAKRREMHEKLQSGAMDILIGTHALLEDPVVFKN
                     LGLVIIDEQHRFGVAQRARMWSKSNNPPHILVMTATPIPRTLAMTLYGDLDISVIDEL
                     PPGRKPVKTILFSDASRLKVFGFMKQKIKEGRQIYVVYPLIKESETLDLKHLMDGYES
                     IVRDFPPPEYAVSIVHGKMRPADKDYEMQRFVKKQTQIMVSTTVIEVGVDVPNASVMV
                     IENAERFGLSQLHQLRGRVGRGADQSYCLLMTGWKVSQEARKRLETMVETSDGFKIAE
                     VDLQLRGPGDLQGTRQSGILDLKIADLIRDEKILKYARNLAFEILRDDPELAQEKNRP
                     LAMQLELLKKDFVDWSLIS"
     gene            complement(3246..4691)
                     /gene="ltrA"
                     /locus_tag="PKM34_03050"
     CDS             complement(3246..4691)
                     /gene="ltrA"
                     /locus_tag="PKM34_03050"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNQ82592.1"
                     /translation="MSKVKKAANADNNRHYPPEAGVELRGKEGERSFAPASAEGEDGN
                     KVNASQMLEKVLDRENLNLAYKRVKKNGGSHGVDGMKVDELLPYLKRQGESLKRSLLE
                     GKYRPQPVRRVEIPKPEGGIRLLGIPTVVDRMIQQAIAQVLSPIFEEEFSKYSYGFRP
                     GRGAHQAIEQARQYINEGYKVVVDLDLEKLFDRVNHDKLMYLLAKRITDKRILKLIRA
                     YLESGVMLGGLISPSEEGTPQGGPISPLLSNVMLHELDKELERRGHRFCRYADDSNIY
                     VKSKKAGNRVMESVSQFIEKRLKLKVNREKSAVDYPTKRKFLGYSFYYNRGVASIRVH
                     AKPLSKLKAKLKKLTGRSIGISMEARTDKLNQTIRGWVNYYQLADMGKHCQKTDEWLR
                     RRLRMCYWKQWKNIGARHYNLVRLGIPDSKAWEFANTRNGHWHTANSPILASTLTNEF
                     FRKLGLTGLSDVYLKSLSLRTAVCRTARTVV"
     gene            complement(5179..6027)
                     /gene="gldN"
                     /locus_tag="PKM34_03055"
     CDS             complement(5179..6027)
                     /gene="gldN"
                     /locus_tag="PKM34_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNQ82593.1"
                     /translation="MKNIALLFISLIILAGISVPAKAQVLSDPPKDVINFDNELLDVK
                     PVPLPAIRQADMMWSKRIWREIDMRQKINQPFYYPIEPHDNWRNFISVIMDALKEGTI
                     TAYDISNTDEFTIPLNYQEIISRQIDTMHKTFQRPYPPYEEYDTVIYSEFNPSKVTRI
                     RIKEDWYFDKKHSQMLVRILGICPVMTKERNNEEYNEALFWIYFPEARPVLAHAQVFN
                     RFNDASRRTYDEIFIKRLFNSYIYKEQNVYDRRIQEYAQGLDALLEAERIKMELLNFE
                     QSLWEY"
     gene            complement(6044..>7549)
                     /locus_tag="PKM34_03060"
     CDS             complement(6044..>7549)
                     /locus_tag="PKM34_03060"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HNQ82594.1"
                     /translation="FENKLGNLYMDFEKQYSLKPQKVGDYWEKAQKARKLSNELKNYV
                     DHVIFDVVRQSEGKDSVKLMQDAYEMRTMRDPYNPSKDIQVPLLNMNNVATKDKYDES
                     TNYFINKGKAEELKERIEKYKADMIALVPEQFRQNIQLGLETDGDYYNADGEKQTWEL
                     HHFYHTILAASVTILNKIKAEVQTAEFDITAELLSEIDISDFKFDKIEAKVIPTTNYV
                     LQGNKYEAEVLVAAFDTRQTPEVFVLQGAEEITVSNKDRAQKVAGEQGVVKLSFPSGN
                     VGPQKYAGVVEVVSPEGERIPYNFSGEYVVAPPSLTVAATKMNVFYIGVDNPVSISVP
                     GIADANLKPSISTGTLTRDPEGKDWIVKVPQGQPKAIISVEALYQGETVPMGSKEFRV
                     RRVPDPRAEIAGQVEGPIDKNTLLAAGAIIPVMKDFEFDLYFEVKSFRLTTIIGGDGI
                     SKRANNNRLSDEMISMIRSARSGQKFFFENITAEGPDKTPRSLNPITLEIK"
BASE COUNT         1672 a         1948 c         1716 g         2213 t
ORIGIN      
        1 cccgatccgg cgcctccaaa aatcccggcg atgcacatcc cttcagactc attgcagatc
       61 atcaggtcat ctccggagag ttttctttcg gtttcatcga gagtaacaaa caattcatcg
      121 ttcaacgctt tgcgaaccac tacttttccg cccctgatcc gggatgcgtc gaaagcatgg
      181 agcggctgcc ccagctccag catcacgaaa ttggtcacat cgaccacatt gctgatcggg
      241 cggataccgg ctgcattcag caggtttttc aaccattcgg gcgattcagc gaccttaatg
      301 ccggaaatcg taacgccgct gtaccgcggg caggctgttg catcggcaac ggtaaccggg
      361 atgatccgat tgtggttatc gaccgcaaat tcttcgacgg atggccattt cagtgcatat
      421 ttcctttcac cggcttcccg gttgagtact gccacgaggt cgcgggccac gcccaggtgc
      481 gacattgcgt cggtccggtt cggcgtcagt ccgatttcga aaatataatc ttcggaaaca
      541 tgaaaatatt cggcggccgg cataccaatc tcggcctccg gatcgaggac cataatccct
      601 tcatgggatg tacccaggcc cagttcatct tcggcacaga tcattccttc agagaactcg
      661 cccctgatct ttgcttcctt tatctcgaaa gatttatcac cctggaaaag gatcgtaccg
      721 actgttgcga ctgggacttt ctgaccagcc ctgacattgg gcgccccgca aacgatcgac
      781 agcagccttc caatacctgt atctactttg gtcaggctca gtttatccgc gttagggtgt
      841 ttctcacatt cgaccaccag cccgacacat acgcctttca gaccgccttt taccgtttcg
      901 gtcttttcat aatcttccac ttcaaggcct gaagaagtca gttttctggc cagttcttcc
      961 ggttccaggt cgagatcgac atatcgccgg agccagttat aggatatttt cataccggat
     1021 taagtaacga ttcggggcaa agttaagaaa attgcttttc gggcgcttac tattaactga
     1081 tgaggctcca gtcgacgaaa tcctttttca gaagttccag ttgcatggcc aggggtctgt
     1141 ttttttcctg tgccagctca gggtcatccc ggaggatctc aaaggcgagg ttccgggcgt
     1201 atttcagaat cttttcatca cggatgaggt ctgcaatttt cagatcgagg ataccgcttt
     1261 gccgggtacc ttgcaaatcg ccggggccgc gcagctgaag atcgacctct gcaattttga
     1321 aaccatcgct ggtctcgacc attgtttcca gccttttcct ggcttcctgc gacactttcc
     1381 agccggtcat cagcaggcag tatgactggt cggctcccct tccgaccctg ccgcgcaact
     1441 ggtggagttg tgacaagcca aaccgttcgg cattttcaat gaccataacc gaagcatttg
     1501 ggacatcaac tcccacctcg attaccgttg ttgataccat gatctgggtc tgcttcttta
     1561 caaagcgttg catctcgtaa tccttatcgg cgggtcgcat tttgccgtga acgatactca
     1621 ccgcatattc cggcggcgga aagtcacgga cgatgctttc gtacccatcc atcagatgtt
     1681 tcaggtcgag ggtttccgat tctttgatca acggatagac gacatagatc tgccgtccct
     1741 ccttgatttt ctgcttcata aaaccaaaga ctttcagcct ggaggcatca ctgaataaga
     1801 tcgtttttac cggtttccgg ccgggtggca gctcatcgat gaccgatata tcgagatcac
     1861 cataaagggt catagccagt gtccggggaa taggcgtggc tgtcatgacc aggatatggg
     1921 gcgggttatt gcttttgctc cacatcctgg cccgctgggc gactccaaag cggtgctgct
     1981 catcgatgat caccaggccc aggtttttaa atacaaccgg gtcttccagc agggcgtgcg
     2041 tacctatcag tatatccatg gctcctgact gcaatttttc atgcatctca cgccttttcg
     2101 cagcgggagt cgatccggtc agcaggccga tgccgatccc caggccatcg gtcatacggg
     2161 taagtgtggc gaggtgttga ctggccagga tctccgtcgg ggccatcagg cacgactgaa
     2221 acccgttatc gatggctatc agcatcgcca tcaacgccac cagcgtttta ccgctgccca
     2281 catcaccctg taacaggcgg ttcatctgtt taccggctcc caggtctttc cggatttcac
     2341 gaataacctt tttctgtgct tcggtaagat taaaagggat tttttcactg taaaaccggt
     2401 ttactttgtc agcaacgcgg gaaaatacaa atccgctgat cctttgttgt ctgatcatct
     2461 tatccttcaa taatcccagc tgaatgaaga agagctcttc aaacttaagc cgggcctgtg
     2521 ctttttgcag aagttgctgg ttcaacggaa aatgtatatt gcggagggct tcctcacgac
     2581 ccagcagcga tagtttgcgg ataatttcat ccgggagggt ttcatggagt ttcccttcgg
     2641 cctgaatgat cagttcccgg attagcttca ccaggttttt attgttaagc ccccggtttt
     2701 taagcttttc cgttgtgctg tagatgccgt gaagtgtttg gggcacagtt cgcaagaaat
     2761 cttcggcgcg ttcaatttcc ggatgggcga tgttatatcg ccggttgaag atagtcggtt
     2821 tcccaaacac aatgtattcg acattgggca ggatcgtccc ttcgatccat ttaattccct
     2881 ggaaccagac cagttcaatt tccccggtat catcgcgcag cagagccgac atccgtctgg
     2941 ctctcttttc accgatgatt cgtatattgg ttactgttcc cctgatctgc acataggctg
     3001 tatcgggttc gatctccgaa attttgaata tacggcttcg gtcaatatac cggaacgggt
     3061 aataattcag cagatcgccg aagtgatgaa tattgagttc tgatctgagc aattccgccc
     3121 ttttggggcc cactcctttc aggaattcca ccggggtttg aataaaatct cttgtcatcg
     3181 cgcctgaata tgagtatata caaaaatcga gtaggaagtg agggataagt accctcactg
     3241 tcctctcaca ccaccgtacg tgccgttcgg catacggcgg ttcgtaaaga taaagatttt
     3301 aaataaacat cggaaagacc tgtaaggcca agtttacgga agaactcatt ggtaagagta
     3361 ctggcaagaa tagggctatt ggcagtatgc cagtggccat ttctcgtatt agcgaactcc
     3421 catgccttgg aatcagggat acctaatcgg acaagattgt aatgtcttgc cccgatattc
     3481 ttccattgtt tccaatagca catgcgtagt ctgcgtcgca gccattcatc ggtcttttga
     3541 caatgtttgc ccatgtcagc caactgataa taattaaccc atcctcgtat tgtctggttg
     3601 agtttgtctg ttctggcttc catgcttatc ccgatgcttc ttccggtcag tttcttaagc
     3661 ttggctttta gcttgctgag tggtttggca tgtactctta tacttgccac tcccctgttg
     3721 tagtaaaagc tatatcctaa gaacttccgc tttgttgggt agtcaactgc acttttctcc
     3781 cggttgactt tcagtttcag tcgcttttca ataaactggc ttacgctttc catcacccga
     3841 ttccccgctt tcttgctttt gacatagata ttactgtcat ccgcataacg gcagaaccgg
     3901 tggcctcgcc tttccagttc cttatctagt tcgtgaagca ttacattgga tagcagcggg
     3961 cttatcggcc ctccttgagg tgttccctct tccgaaggac ttatcagccc gcccagcatt
     4021 acccccgatt caaggtatgc ccggataagt ttcaatattc gcttgtctgt tattcttttg
     4081 gcaagaagat acatcagctt atcgtggtta accctatcga agagcttctc taaatccaaa
     4141 tctactacta ctttataccc ttcattaatg tactgccggg cctgttctat ggcctgatgt
     4201 gcaccgcgtc cgggtcggaa gccgtaactg tacttagaaa attcttcctc aaaaatcggg
     4261 gacaatacct gcgctattgc ttgttgtatc atccggtcta ctaccgttgg aatgcccaac
     4321 aaccttattc cgccttccgg cttgggtatt tcaaccctgc gtaccggttg cggtcggtac
     4381 ttcccttcca gtagggatcg cttgaggctc tccccctgtc gtttcaggta aggcaaaagt
     4441 tcatccacct tcatcccgtc cactccatgg ctgccgccgt tcttctttac ccgcttgtaa
     4501 gcaagattca ggttttccct gtccagaact ttttcaagca tctgactggc gtttacttta
     4561 ttcccgtctt ctccttctgc cgacgctggg gcaaagctcc gctctccctc cttacctcgg
     4621 agttccactc ccgcttccgg tgggtagtgc cgattattgt cggcattggc tgctttcttt
     4681 accttgctca caagtcattc aaaatttaaa gacatcttta cgttcagccc ttccctgtgc
     4741 atacatgtct gcccaggtaa tatggcctct gctgactcct gaccattcaa ctatacatcg
     4801 ctgtacaggt tgccggttcc gtatttcatc cccagcctat gggtcaggtc tccccgggta
     4861 agaacgatag ctttcatccc atatacctgc tgcatttact ccaaggggtt cgggcagtat
     4921 tggaatttgc tttgttatgc aagctcgtcc gtccctcata agccttatat gcagttcttg
     4981 tccatcaggc cgggattttg ccgccggctt ccttcagatt ccacctcacg atggacaccc
     5041 ttgcctttgg ctaacacttc ctactgccaa gtgtgttcgg gtctttcacc ctatagttat
     5101 cgcccatgcc gggcgcacat aaaaaaaagc cgcctcagat ggttgggcgg ctttcgggtg
     5161 ttaaatatgg ctaagttttt agtattccca gagactttgc tcgaagttca ggagttccat
     5221 tttaatcctt tccgcttcga gcagggcatc gaggccctgc gcatactcct ggattctgcg
     5281 gtcgtacaca ttttgttctt tataaatata actgttaaac agccgtttaa taaatatttc
     5341 gtcgtaggtt ctgcgcgagg catcgttaaa acggttgaag acctgggcat gagccagtac
     5401 cggtcttgct tccgggaaat aaatccagaa aagggcttca ttgtattcct cgttattcct
     5461 ttctttggtc ataaccgggc aaataccgag tatcctgacc agcatctggg aatgtttctt
     5521 atcaaaatac cagtcttcct taattctgat tcgggtaact ttactgggat taaactcact
     5581 atagataacc gtatcgtatt cttcataagg tggataaggc cgctggaagg ttttgtgcat
     5641 ggtatcgatc tgcctggaaa tgatttcctg gtaattcagc gggatagtaa attcatccgt
     5701 attggaaata tcataggctg taatcgtccc ctctttgagt gcatccataa tgacactgat
     5761 gaagttgcgc cagttatcgt gtggttcgat cgggtaatag aagggctgat tgatcttctg
     5821 ccgcatatcg atttcccgcc aaattcgttt tgaccacatc atgtcggcct gacggatggc
     5881 gggcagaggt actggtttga catccaggag ctcattgtcg aaattgatga catctttcgg
     5941 cgggtcgctg agcacctggg cctttgcagg cacagagatt cctgccagga tgatcaatga
     6001 gatgaaaagc aatgcgatat ttttcatagt gcctccggtg gtattattta atttccaaag
     6061 ttatagggtt caggcttcgg ggtgttttat ccgggccttc agccgtaata ttttcaaaga
     6121 agaatttctg accgctccgg gctgatctga tcatactgat catttcatcg gataaccggt
     6181 tattattggc gcgcttggat atcccgtcgc ccccgatgat agtggtaagc ctgaaagatt
     6241 tgacttcaaa atagagatcg aattcgaaat ctttcataac agggataata gccccggcag
     6301 ccaacagtgt gtttttatcg atcggacctt ccacctggcc ggcaatttca gccctgggat
     6361 ccgggactct cctgacccta aactctttag agcccattgg taccgtttct ccctggtaaa
     6421 gggcttcgac agatataatg gctttaggtt gaccttgcgg aactttgaca atccagtctt
     6481 taccctcagg gtctcgtgta agggttccgg ttgagatcga aggcttcagg ttggcatctg
     6541 caatcccggg aacggagatt gaaacggggt tatccacacc gatgtaaaat acgttcattt
     6601 tagtggctgc aaccgtcaat gaaggaggag caaccacata ttcaccggaa aagttgtagg
     6661 ggatccgttc tccttccggg gataccacct caacgacacc cgcatatttc tgggggccga
     6721 catttcctga ggggaatgac agcttcacca caccttgttc accggcgact ttctgtgcac
     6781 ggtccttatt gcttacagtg atctcctcgg caccctgaag cacgaaaact tccggggttt
     6841 gccgggtatc aaaagcagca acgagaactt ccgcttcgta cttgttaccc tggaggacat
     6901 agttcgtcgt ggggatgacc tttgcttcaa ttttatcgaa cttaaagtcg gaaatatcaa
     6961 tttcgctgag caactcggct gtgatatcga attcggcggt ctgtacttct gctttgattt
     7021 tattcaggat cgttaccgat gctgccagga tggtatggta gaaatgatgc aattcccatg
     7081 tttgtttttc gccgtctgca ttgtaataat ccccatccgt ttcaaggccc agttggatat
     7141 tttgcctgaa ctgttccggt acgagagcga tcatatcggc tttatatttc tcgatccttt
     7201 cttttaattc ctccgccttg cctttgttta tgaaataatt ggtcgattcg tcgtatttat
     7261 ccttggtagc gacattattc atattcaaca gggggacctg gatatcctta ctcggattgt
     7321 aagggtcgcg catcgttctc atttcatatg cgtcctgcat caatttgaca gaatctttcc
     7381 cttccgattg tcggaccaca tcaaagatca catgatccac ataattttta agttcattcg
     7441 ataattttcg tgctttctgt gctttttccc agtaatcgcc aactttctgc ggtttgagtg
     7501 aatactgctt ctcaaaatcc atataaaggt ttccaagctt attttcaaa
//
LOCUS       DAOQXS010000065         7422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10361_length_7422_cov_3.596308, whole genome shotgun sequence.
ACCESSION   DAOQXS010000065 DAOQXS010000000
VERSION     DAOQXS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10361_length_7422_cov_3.596308"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PKM34_03065"
     CDS             <1..1110
                     /locus_tag="PKM34_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ82595.1"
                     /translation="YHHHDFVPRITEPSDPAYLLFTSGTTGEPKGVPVSCANVISYLD
                     FMLETYDFERDDRFTQIFDLTFDLSVHDMLLAWSIGACLVVPDDSSSFAMSRFIRDKK
                     PTVWFSVPSVPALMNRMRLLKPDTFPSLRLSFFCGEALMAGDARAWKFAAPNSSLINL
                     YGPTEATIAISGYSLPGDETKWKTALGIVSVGKVFDGNKYLILRENPSDARGELCLCG
                     NQVVEKYLDNEEADKQSFFTDADDMERYYKTGDMVSVDEQGDLFYLGRKDSEVKISGY
                     RVNLKEVENVLLESETVTQAVVVYHDSSMSLLAFLVLSEIPPGDALQKLTEYCQSRLP
                     WYMVPGKFIFVKEIPLNDNGKIDRKQLLKKYDEGN"
     gene            1094..1348
                     /locus_tag="PKM34_03070"
     CDS             1094..1348
                     /locus_tag="PKM34_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82596.1"
                     /translation="MMKEIEIRNQVMALIHDKLARLSVKESELGYPFDLVKSGLLNSL
                     EFVDLVASLEKENDREIDFEKELATGAFTTLGGLIKAFSS"
     gene            1353..1838
                     /locus_tag="PKM34_03075"
     CDS             1353..1838
                     /locus_tag="PKM34_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ82597.1"
                     /translation="MEKIEFIRLDNAELPDWDEIPGLFNEMYARMDEMGLMVPLAADG
                     AQKWLRSTRNTAGKFGIVVLARIGEVPVGFAHGMIKFLPDYLGNHSVGLITHVFVKDI
                     ARKKGLGRELVKMLEGWFHDKNVHSIELQVINDNLPAQKFWENLGYRVELTQYRKIRS
                     K"
     gene            1835..2695
                     /locus_tag="PKM34_03080"
     CDS             1835..2695
                     /locus_tag="PKM34_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNQ82598.1"
                     /translation="MINPYYYHDMIRQLDIRPGSLVILAGDVTRLALAGKRMEIGFDI
                     HTFTGVVKQCLGKGGTLVIPAYNFNLRNHDYFHPKKSLPITGSLAVAALKRPEFIRTS
                     HPLHSFLVWGEQAGALAALSNSSSFDERSPFAFFRDHKALMLLVDTTIAEAFTFVHHV
                     EEMEKVKYRRYKKLIINVEGEDGQIAGREVLIYAKKPGWTMDMTGLEEILFRENMAKK
                     QTINKISFIQVDLDAAYPLILDDIRNNRGRNISRFSYNLFFREMSKSVMQSFGFRTLG
                     DKISHDHGLL"
     gene            2676..3989
                     /locus_tag="PKM34_03085"
     CDS             2676..3989
                     /locus_tag="PKM34_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNQ82599.1"
                     /translation="MITVYCDHITPRHHYIFRFIFEDVMGTEFRFISGIDDFLQDKGP
                     KISYSKENPGGGIHFHPYPILSQNGITTQDTETFLWKGLPVFFKVIKPSEWPFDPFSL
                     AFYLVSRYEEYLPFEPDRHGRFRPEESLAFKGGFLGIPLVDMIVKELKSLISSRYPGY
                     AFPVMPFRFVPTIDVDIAFAHRGKGWLRAAGAWIKLLLRADFRQLKERIFVVSGKIPD
                     PYDNFDLHLETAGKSGLSLRYFILIGDFGRYDRNTSYSSSSFKELLKRLSLTADVGIH
                     PSYRSHLRKEIFIKEKKRLEEITGNQVMASRFHFLRVRFSGSFRILIDQGITDDYSLG
                     YSTVNGFRAGTCTPFLFYDIDKEEITKLRIHPFIFMDSAFIDQMKMSPAEATGQIREL
                     VKRVKELGGEAIGIWHNYSLSEKGQYKGWQEVFRTTFKEFQNDNR"
     gene            3986..4921
                     /locus_tag="PKM34_03090"
     CDS             3986..4921
                     /locus_tag="PKM34_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82600.1"
                     /translation="MIRYIDRKDIDDEKWNACLERAFNGNLYGRSWFLDIVADNWAGL
                     VENDYERIFPLVFRRKFGLSYIYQPFFTQQLGLFSTTRLDAGKLEEFIGAIPSRFRRA
                     EMNLNTLNNPSGTHYKIIPQVNHELDLINSYEKIRQGYSENLVRNLKKAEKAGLSVSR
                     NIKPDDIIALFRQNRGKDIGHLGDTDYMRLKRIAYTCMYKGMAGIQGVYDDRNQLVAG
                     AFFIRSHNKAVFLFSGLSDEGRKSGAMPFLIDAYLRHNAGKHLTFDFDGSNDADLARF
                     YKSFGSREVHYQRVIIDRMPALLKIGVYLYKKIKA"
     gene            4918..6159
                     /locus_tag="PKM34_03095"
     CDS             4918..6159
                     /locus_tag="PKM34_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82601.1"
                     /translation="MNLLIRENIRISVESIRSHMLRTILTITIIAFGIMALIGILTAI
                     DAIKYFLNDNFMMMGANTFSIRNREMMIQIGGRSNDPKRYKRITFDQAMQFKEDFDFP
                     AHVSISAFASHTGTLKYKSEKTNPNVAVLGTDENYIITAGQEIVKGRNFNPNEVYFGA
                     HVVIIGSEVEKDIFKNKEDPVGKIISVGPGKYQVIGVLKEKGSSMGFSGDRQVLLPLT
                     NVDQYFPRPNRSYGIDVSTIDPLQMDQCIGEATGLFRIIRGDRVGEEQTFDIERSDSL
                     AAMLTENIKYVTMAATFIGLITLLGAAIGLMNIMLVSVTERTLEIGIRKAMGATQRTI
                     RNQFLAEAIVIAQMGGILGIIFGIAIGNTLSLIMKTSFIIPWLWILLGVTLCFIVALA
                     SGIIPANKAAKLDPIESLRYE"
     gene            6402..6686
                     /locus_tag="PKM34_03100"
     CDS             6402..6686
                     /locus_tag="PKM34_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNQ82602.1"
                     /translation="MDCNNLVFSEHIISQMFKRNISVDDVKGILEVGEIIMTYPDDKP
                     YPSYLMLGFLNKRPLHLVVAKNNQAGLCIMVTVYEPDKNSWSLDFKIKIR"
     gene            6688..6915
                     /locus_tag="PKM34_03105"
     CDS             6688..6915
                     /locus_tag="PKM34_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HNQ82603.1"
                     /translation="MNCVICKNGQTQKGKVIVTLENNGAIILVKDVPAHICDVCEHYF
                     LDEDIAREVLKRGQESLSKGTELEVIRLRVA"
BASE COUNT         1949 a         1734 c         1838 g         1901 t
ORIGIN      
        1 tatcaccatc acgactttgt ccccaggatc accgagcctt cggatccggc ttacctttta
       61 ttcacctcag gtactaccgg cgaaccgaaa ggggtaccgg tctcctgtgc caatgtcatt
      121 tcgtacctcg atttcatgct cgaaacgtat gattttgagc gtgatgaccg ctttacccag
      181 atcttcgacc tgacattcga cctgagcgtc cacgatatgc tcctggcatg gtcgattggc
      241 gcctgcctgg tcgtccccga cgatagttcc tcctttgcca tgtcgcggtt tatccgggat
      301 aaaaaaccga cggtatggtt ctccgtcccc tccgtgccgg cgttgatgaa ccggatgagg
      361 ttgcttaaac cggatacatt tccttcgctg cgcctgagct ttttctgcgg cgaagcgctg
      421 atggccggtg atgcccgggc ctggaaattc gcagcgccca attcctccct catcaacctc
      481 tatggcccta cagaggctac aattgctatc tccggctata gccttcctgg tgatgaaacg
      541 aaatggaaaa cagccctggg aatcgtctct gtcggcaaag tgttcgatgg caacaaatat
      601 ctgatcctgc gcgaaaaccc gtccgatgcc cgtggtgaac tttgcctgtg cggaaaccag
      661 gtcgttgaaa agtacctgga taatgaagaa gccgacaaac agtcattttt caccgacgcg
      721 gatgacatgg aaagatatta taaaacgggc gacatggtga gcgtcgatga acagggcgac
      781 ctcttctacc tggggagaaa agacagcgag gtgaagattt ccggttaccg cgttaacctg
      841 aaagaggtcg agaatgtgct cctggaaagc gaaactgtta cacaggccgt tgtggtctat
      901 catgattcat ccatgtcact gctggctttc ctggtattat cagaaatccc gcctggtgat
      961 gccttacaga aactgacgga atattgccag tcacgccttc cctggtatat ggtgccggga
     1021 aagtttatct ttgtgaaaga aatcccgctg aacgataacg gtaaaattga ccggaaacaa
     1081 ctgctgaaaa agtatgatga aggaaattga gatccggaac caggtgatgg cgctcatcca
     1141 tgacaagctt gcccgcctgt cggtaaaaga aagtgaactg gggtatccgt tcgacctggt
     1201 gaaaagcggc ctgcttaatt cgctggaatt cgtcgacctg gtcgcttccc tggaaaagga
     1261 aaatgaccgg gagatcgatt ttgagaaaga gcttgcgacc ggagctttta ccacgctggg
     1321 cggactgatc aaagcatttt catcctgaag ccatggaaaa gatcgaattc atccggctcg
     1381 acaatgcgga acttcccgat tgggatgaaa tcccagggct tttcaacgag atgtacgccc
     1441 ggatggatga gatggggctg atggtgccgc tggccgctga cggagcgcag aagtggctgc
     1501 ggtcgacccg gaatacggcg gggaagttcg gcatagtcgt cctggccagg ataggagagg
     1561 ttccggttgg ctttgcccac ggcatgatca aattcctgcc cgattacctt ggaaaccata
     1621 gcgtgggatt gatcacgcac gtttttgtca aagatatagc caggaaaaag gggctgggcc
     1681 gagaattggt gaaaatgctg gaaggctggt ttcatgataa aaatgttcat tccattgaac
     1741 tacaggtaat taatgataat ttacctgcac agaagttctg ggaaaacctg gggtacaggg
     1801 tggaattgac gcaataccgc aaaatccgtt cgaagtgata aatccttact actaccacga
     1861 tatgatccgg caactggaca tacgtccggg tagtttggtc atcctggcgg gggatgtgac
     1921 ccggctggcg ctggccggca aacggatgga gatcggtttc gatatccata cgtttaccgg
     1981 tgttgtgaag caatgcctcg gaaagggagg cacactggtt atcccggctt ataatttcaa
     2041 ccttcgcaac cacgactact tccatccgaa aaaatcgttg cccataacgg gttcactggc
     2101 ggtggcggcg ctgaaacgcc ctgaattcat ccggacgagc catccgctcc attcattcct
     2161 ggtttggggc gagcaggccg gtgccctggc cgcgctttcg aacagcagct ccttcgatga
     2221 gcgctccccg tttgcatttt tccgtgatca caaggcgctg atgctgctcg tcgatacaac
     2281 catcgccgaa gcctttacgt ttgttcacca tgtggaagag atggaaaaag tgaaatacag
     2341 gcgttataag aagctgataa taaatgtgga aggtgaggat ggccagatcg ccggcaggga
     2401 agtattgatc tatgcaaaga aaccgggctg gaccatggat atgacaggcc tggaggagat
     2461 tttattccgg gaaaacatgg ctaagaagca aacgatcaat aaaatcagtt ttattcaggt
     2521 agacctggat gcagcttatc ccctcatcct cgacgatatc cggaacaacc ggggccggaa
     2581 tatttcgcgg ttcagttata acctcttttt cagggagatg tctaaaagcg tcatgcaatc
     2641 cttcggtttt cgtactttag gcgataaaat cagccatgat cacggtttat tgtgaccata
     2701 tcacccccag gcaccactac atcttccgtt ttatttttga agatgttatg ggtacagaat
     2761 tccggtttat ttccggtatt gatgattttc tccaggataa aggcccgaaa ataagttatt
     2821 caaaagaaaa cccgggcggc ggtatccatt tccatcctta tcccatcctg tcgcagaacg
     2881 ggatcactac gcaggatacg gagacttttt tatggaaggg tctccccgtt tttttcaaag
     2941 taatcaaacc ttcggaatgg cctttcgacc cgttttccct tgcgttttac ctggtcagcc
     3001 ggtacgaaga gtatctgccg tttgagcccg accggcacgg caggttccgg cccgaagaga
     3061 gcctcgcttt caaaggcggt ttcctcggta ttccgctggt cgatatgatc gtgaaagagc
     3121 ttaaatcgct gatcagcagc cgttatcccg gttatgcttt cccggtcatg cctttccgtt
     3181 tcgtcccgac gatcgatgtg gatatcgcct ttgcccatcg ggggaaaggc tggctcaggg
     3241 ccgcgggcgc ctggatcaaa cttttgctcc gggccgattt ccggcaattg aaagagagga
     3301 tattcgtcgt ttcagggaaa ataccggatc cgtatgataa ttttgatttg cacctggaaa
     3361 cagccggaaa atcgggcctt agtcttcgtt atttcatcct catcggggat tttggaagat
     3421 atgaccgcaa tacatcgtat tcatcatcat cttttaaaga attattaaaa agattaagcc
     3481 ttacggcgga tgtcgggatt cacccttctt accgctccca tctccggaag gaaatattta
     3541 tcaaggaaaa aaagagactg gaggaaatta ccggaaatca ggtcatggct tcccgctttc
     3601 attttctcag ggtcaggttc tccggttctt tcaggatttt aattgatcag gggattacgg
     3661 atgattattc gctgggctat tccacagtga acggtttcag ggcgggcacc tgtaccccct
     3721 tcttgtttta tgatattgat aaagaagaga ttacaaagct taggatccat cccttcattt
     3781 ttatggattc ggcatttatt gaccaaatga aaatgtcgcc ggcggaagca accgggcaaa
     3841 taagggaact tgtaaaacgg gtgaaagaac tcggtggcga ggccatcggt atctggcaca
     3901 actattccct ttccgaaaaa gggcaataca aaggctggca ggaagtattc aggactactt
     3961 ttaaggaatt tcagaacgat aatcggtgat caggtatatc gacagaaaag atattgacga
     4021 tgaaaagtgg aacgcctgcc ttgaaagggc gttcaacgga aacctctatg gcagatcgtg
     4081 gttcctggat atcgtggcgg ataactgggc cgggctggtt gaaaatgact atgaacggat
     4141 ctttcccctc gttttccgcc gaaaattcgg tttatcctat atttatcagc ctttctttac
     4201 ccagcagctc gggctatttt cgacaacaag gctggatgcc ggaaagctcg aagagttcat
     4261 cggggctatc ccttcccgct tcagaagggc cgaaatgaac ctgaatacgc tgaataaccc
     4321 ttccggaact cattataaga tcattcccca ggttaaccac gaactcgacc tgatcaacag
     4381 ttatgaaaag ataaggcagg gttattccga aaacctggtc cggaacctta aaaaagcgga
     4441 gaaagcgggt ctttcggtct ccaggaatat caagcccgac gacatcatcg cgttgttcag
     4501 gcaaaaccgg gggaaggata tcggccacct gggcgatacg gactatatga ggctgaaacg
     4561 gatcgcctat acctgcatgt acaagggaat ggccgggata cagggcgttt acgacgaccg
     4621 caaccagctc gtagccggcg ctttctttat ccggagccat aacaaagctg ttttcctctt
     4681 ctcaggcctt tctgacgagg gccgcaaatc gggagcgatg cctttcctga tcgatgccta
     4741 cctcaggcac aacgccggaa agcacctgac cttcgatttc gacggttcca acgacgctga
     4801 cctcgcccga ttctataaaa gcttcggttc cagggaagta cattatcagc gggtgattat
     4861 cgaccggatg ccggcattat taaaaattgg tgtttatctt tacaaaaaaa taaaggcatg
     4921 aacctcctca tccgggaaaa catcaggatt tcggtcgaat cgatccgcag ccatatgctc
     4981 cggaccatcc tcaccattac catcatcgct ttcgggatca tggcgctgat cgggatcctg
     5041 acggctatcg atgccatcaa atacttcctg aacgacaact tcatgatgat gggggccaac
     5101 accttttcga tccgcaaccg ggagatgatg atccagatcg ggggtcgaag caatgacccc
     5161 aagcggtata aaaggatcac tttcgaccag gccatgcagt ttaaagagga ttttgatttc
     5221 cctgcccacg tctccatatc cgcttttgcc tctcataccg gcacactcaa atacaagtcg
     5281 gagaagacca atccgaacgt agccgttttg ggaaccgatg aaaactatat cattaccgca
     5341 gggcaggaga tcgtcaaggg gcggaatttc aatccgaatg aggtctattt tggcgcccac
     5401 gtggtgatca tcgggagcga ggtggagaag gatattttta agaacaagga agatcctgtg
     5461 ggaaagatca tatcggtagg gcccggcaag taccaggtga tcggcgtgct gaaggagaaa
     5521 ggctccagca tgggcttttc gggcgaccgg caggtgcttc ttcctctgac caatgtcgac
     5581 cagtactttc cccgtccgaa ccggtcatac ggcatcgatg tttcgaccat tgacccgctt
     5641 cagatggatc agtgcatcgg tgaggccacc ggcctgttcc ggatcatccg gggcgaccgg
     5701 gtgggagagg agcagacctt cgatatcgag cggagcgact cgctggccgc catgctgacg
     5761 gaaaacatca aatacgtgac gatggccgcc accttcatcg ggctgatcac gctgctgggc
     5821 gccgctatcg gcttaatgaa catcatgctg gtatcggtca cggaacggac cctcgaaata
     5881 ggcatccgca aggcgatggg cgctacgcag cggaccatcc gcaaccagtt cctggcagaa
     5941 gccattgtaa tcgctcaaat gggaggtatc ctcgggatca ttttcggcat cgccatcggc
     6001 aacaccctgt cgctcatcat gaagaccagc ttcatcattc cctggctctg gatattgctc
     6061 ggcgtcacgc tttgtttcat cgtcgccctg gcctcgggaa tcatccccgc caacaaagcg
     6121 gcaaaactcg acccgatcga atcgctgcgc tacgagtgag cggtgaaggg tgaagagtga
     6181 acggtgagca gtgaagtgtg aacggtgaac ggtgaatggt gctgttcaat gtgaaataat
     6241 gaatttagct gaacccagta tgccagtttc attcctgaca acaaccaggt atatcccagc
     6301 cggcagcccg gagacataga tttgtcaaat caattgtgtg tttttctaat tgctttcatt
     6361 ttccacagaa ttagcttact tttgtattga attgagtctc gatggattgt aataacctgg
     6421 tattcagtga acatattatc tcccaaatgt tcaaacgcaa tatttcggta gatgatgtaa
     6481 aaggcattct tgaagttggt gaaattatca tgacatatcc tgatgataag ccttacccaa
     6541 gttatctgat gcttggtttt ctgaataaaa ggccgttaca tttagtggtt gctaaaaata
     6601 accaggctgg tctttgtata atggttactg tttatgaacc agataagaat tcttggtcac
     6661 tcgattttaa aattaaaata agataaaatg aactgcgtaa tttgtaaaaa cgggcagact
     6721 caaaaaggta aagtcatcgt taccctcgaa aataatggtg ctattatcct ggtcaaagat
     6781 gttcctgcac atatctgtga tgtttgcgag cattattttt tggatgaaga tattgcccgg
     6841 gaagttttaa aaagagggca ggaatcgctt tcaaagggca cagaacttga agttatcagg
     6901 ctaagagtag cctgatacgt tgctttaaaa cttgagtttc ccctgattct tcaatcgaca
     6961 tttaatcata taggtttatc atatatattt tctacacagt ttgacaatat tctttcgcgt
     7021 cgtcaaacaa tccgaagtat tgaagggtgt ggcgttaagg gtaaatcatg aaaaatgcaa
     7081 aatgccatgc aaaatgaaaa tcgcaaaacg taaaatgcga acatcttagg ggtccctttt
     7141 aagacaattt attttctttt cattattcat ataagattga acaaaatatg attaacttta
     7201 accattcata gacttttacg atgaaaagaa gattcctaat atttttaatc atatcccaca
     7261 tcattttgaa ttttagcccg tgttattccc aggaatttga gatgcccggc tatatttatg
     7321 atattaatac aggtgatatt gatgcaaatg gcgcaaatta tattcttgtt tcctgcccat
     7381 atactgatac cataacaatt ttatataatg atggagtggg aa
//
LOCUS       DAOQXS010000066         7390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10429_length_7390_cov_3.774642, whole genome shotgun sequence.
ACCESSION   DAOQXS010000066 DAOQXS010000000
VERSION     DAOQXS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10429_length_7390_cov_3.774642"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKM34_03110"
     CDS             complement(<1..1034)
                     /locus_tag="PKM34_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNQ82604.1"
                     /translation="MSLSDKVRQKPWIGWAIFIATAVVVFFLGLLANSVMERRTEAQF
                     AFSTAVKYDNSEPRNAVWGKNFPREYQSYLQTSDTSFRSKYNGGAMIDMLEVDPRLVV
                     LWAGYGFAKDYNQGRGHYYAIEDIRNTLRTGGPAEGKPSPQPNTCWACKSTDVPRMMK
                     VTGPAEFYNGTWDTKGHEIVNAIGCADCHDAKTMNLQISRPALKEAFERKGIDLAKVS
                     HQEMRSLVCAQCHVEYFFDKREPKHNYLTFPWDNGYTAEDIERYYDSFMFTDWTHALS
                     KAPMLKAQHPDWEVYMTGIHAERGVSCADCHMPYKSEGGQKFTDHHLQSPLNNVEASC
                     AVCHREKTTTL"
     gene            complement(1168..1794)
                     /gene="nrfH"
                     /locus_tag="PKM34_03115"
     CDS             complement(1168..1794)
                     /gene="nrfH"
                     /locus_tag="PKM34_03115"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNQ82605.1"
                     /translation="MQGKIVNYFIPPPQWRLPVIIMLGIFVGLALFTFRTSRAWSYLS
                     DDPETCMNCHIMAPQFATWSHSSHRGKANCNDCHVPHNNFFNKYYFKAKDGLRHATIF
                     TLRAEPQVIFIKEAGVKVVKDNCVRCHEQQMACKHCHNKIIPEGDGIFCEREDEISML
                     NGEFTEYHADRLCWECHRETPHGRVNSISSVPNARVPVPSSNVPEWMK"
     gene            complement(1775..3421)
                     /locus_tag="PKM34_03120"
     CDS             complement(1775..3421)
                     /locus_tag="PKM34_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNQ82606.1"
                     /translation="MRSGPKDCHNGNRWLKFFKKWHRWPGVIMGFFFILWAISGVVMN
                     HRQLFSNFDITRNLVPKENRYVNWNNAAVRSGVMIGNDSLLLFGNTGIWLTDTRFAAF
                     KDFNAGFPEGIDNRKISSMLVSSKGNVYAGTLFGLFYLDFDKEKWVKIPVKFKDERIQ
                     WIIEKEGRIVFLTRSEMFESVDNPASFNPVGLKLPPPAGYDNKTGLFRTIWVIHSGEI
                     YGKIGRLVVDALGLVVILLAVTGSLHFAMPYALRTLKKRKKSLKLASATKKASATWHK
                     KAGIWIAVFILINTITGMFLRPPLLIAIANARVGKIPFSVMDTPNPWEDKLRAVIWDE
                     EIDGYILGTTEGLFFADNSLEREMVPLPGQPPLSVMGINVFQKTGSGQYLVGTFNGIY
                     DWYLKRDGVPPDGVPGTRDPISFYPDGVSTFRDGVSFNHLSGRPPQPSNSNSKPFGDE
                     MVSGYLKLENGNSVYFDYNKGAVSLGNEPVMPAMPLEIIEKSPMSWWNFALEVHTGRI
                     FKPLVGDFYILIVPLLGIFGTVLIISGIIVWIKLYARKNR"
     gene            complement(3425..3943)
                     /locus_tag="PKM34_03125"
     CDS             complement(3425..3943)
                     /locus_tag="PKM34_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNQ82607.1"
                     /translation="MEQLIITPRTKVYELLEAYPQLEDTLIEIAPVFKKLKNPVLRRT
                     IARVTTLQQAAQVGQVPVHEMVNTLRQKVGLDRLEGLEATASGNASKPSWLIEDKIVK
                     RLDARPVIEQGGHPLGEVLTGVGDLKPGEIYELVTPFLPAPLIERVVAQGFDHWSDKK
                     AEDHFINYFLKK"
     gene            complement(4017..5252)
                     /locus_tag="PKM34_03130"
     CDS             complement(4017..5252)
                     /locus_tag="PKM34_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNQ82608.1"
                     /translation="MSELINNSEKRKELLKHMILQLHAGEAPDEARKQLVSLLTKIPY
                     GEVVEVEQELISEGTLSEAEVLKFCDIHSEALDGAIDLSGMKIIPPGHPVDTFKRENK
                     ELLKVVDQLESLYTRVSSVKSHEETAAFLIQLKTLFNSLMDVEKHYLRKENLVFPFME
                     SYGITGPPKVMWGKHNEIRELLKASIEVLNAPGQLSPEEMETVVQMVLKPASDGVADM
                     TLKEDQILLPMVMDKLTDLEWYEVYKQTLEIGFCLYDPDIEWRPENIAAEEVADAGND
                     LVHLPSGSFTKAELQAILNTLPVDMTFVDKNEKVKYFSQGKERIFHRSRAILNRDVKL
                     CHPPSSVHVVEQILEDFRTGRQDHAPFWIQMKGKFIYIEYFALRDENGEYLGTLEVSQ
                     DLTEKRALEGEQRILAYGK"
     gene            complement(5323..5652)
                     /locus_tag="PKM34_03135"
     CDS             complement(5323..5652)
                     /locus_tag="PKM34_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ82609.1"
                     /translation="METPLFSHSQVMNFNDKVEYSAEGIVSKRVIQKEKGNVTLFAFD
                     KGQKLSEHSAPFDAMVQVLEGQAEILINRVPYLLGAGQSIIMPANIPHAVNAVEKFKM
                     LLTMIKE"
     gene            complement(5777..6643)
                     /locus_tag="PKM34_03140"
     CDS             complement(5777..6643)
                     /locus_tag="PKM34_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ82610.1"
                     /translation="MKREIIKVDEEKCNGCGLCIPSCPEGALQIIDGKARLVSDLMCD
                     GLGACIGECPEGAIEIEEREAEPYNEVAVIKEIVKFGENTVVAHLKHLLDHNEMMYFE
                     TAVGYLEENSGSIGFDLEDVLDKVYAKSVGGDCAHGGCPGAKSFERDGVPRDEVPGDA
                     VPSQLRQWPVQMHLINPSAPHFRNAEVLLAADCVAFSVGNFHQKYLKGRSLAIACPKL
                     DQGKEVYVEKLKTMIDEANIKELNVMVMEVPCCSGLVHIANLAREAASRKIQIKKTII
                     GIKGEVLMESYI"
     gene            6958..>7390
                     /locus_tag="PKM34_03145"
     CDS             6958..>7390
                     /locus_tag="PKM34_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ82611.1"
                     /translation="MYQALINSPIFTGLSEEELEKLFQRCHCFTKNYEVGDIAAFSGE
                     PVNFLIIVLEGSVKGEMVDFSGKIIKIDDIRAPYPLASAFIFGKQNKFPVNVMANERT
                     KLLYIPKYDFMKMLQADIRLMQNFLNVISGHTQILASKLKFL"
BASE COUNT         1738 a         1793 c         1805 g         2054 t
ORIGIN      
        1 agcgtagtcg ttttttcgcg gtgacagacg gcgcaggagg cttccacatt gttcagcggg
       61 ctctggaggt ggtgatcggt aaatttctgc cccccttcgc tcttgtacgg catgtggcaa
      121 tcggcacagg agacgccccg ttcggcgtgt atcccggtca tatagacttc ccagtcgggg
      181 tgctgggctt tcagcatcgg cgctttgctc agtgcatggg tccagtcggt aaacataaac
      241 gaatcataat agcgttcgat atcttccgca gtatatccgt tatcccacgg gaaggtcagg
      301 tagttgtgtt tcggttcgcg tttatcgaaa aagtattcga cgtggcactg ggcacagacc
      361 agtgagcgca tctcctggtg ggagacttta gcaaggtcga tgccttttct ttcaaaggct
      421 tctttcaatg cgggccggct gatctgcagg ttcatggttt tcgcgtcgtg gcagtcggca
      481 caaccgatcg cattgacaat ctcatggcct tttgtatccc aggtcccgtt gtaaaattca
      541 gccgggccgg taactttcat catcctgggg acatcggtgc ttttgcaggc ccagcatgta
      601 ttgggctgcg ggctgggttt cccttcggca gggccgccgg tccgaagtgt attccggata
      661 tcctcgatgg catagtaatg cccccgtccc tggttataat ctttggcaaa gccatagcca
      721 gcccaaagga cgaccagtcg cggatcgacc tccagcatgt cgatcatggc accgccgttg
      781 tatttgcttc ggaacgacgt gtcggaagtt tggaggtatg actggtactc ccgggggaaa
      841 tttttacccc agacggcatt tcgcggttcg gagttatcgt actttacggc agtactaaaa
      901 gcaaactgcg cttcggtccg ccgttccata acggaattgg cgagaagtcc caggaagaaa
      961 acaaccactg cggtggcaat gaagatcgcc cacccgatcc agggcttttg acggactttg
     1021 tctgataatg acataatgct taatgtttaa tgagtaatgc ttaatgcttt aatgcttaat
     1081 tcttaatgct taattcttaa tgcttaattc ttaattatca attactaatg accaatgact
     1141 aacgactttt gaccaacaac taatgactca cttcatccac tccggcacat tgcttgaggg
     1201 gacggggacg cgggcgtttg ggacgctgga gatgctgttt acgcggccgt ggggtgtttc
     1261 gcggtggcat tcccagcaaa gcctgtcggc gtgatactcg gtgaattccc cgttcagcat
     1321 gctgatttca tcttcacgtt cgcaaaagat cccatcacct tcaggtatta ttttattatg
     1381 acaatgttta caggccattt gctgctcatg gcatcttaca caattatctt taacaacctt
     1441 aaccccggct tctttgataa agatgacctg gggttcggcc cggagggtaa agatggtggc
     1501 atgtcgcaat ccatctttgg ctttaaaata gtatttatta aagaagttat tatgcgggac
     1561 atggcagtcg ttgcagtttg ctttgccgcg gtgtgagctg tggctccagg tggcgaactg
     1621 gggcgccatg atatggcagt tcatgcaggt ttcgggatca tcggagaggt acgaccaggc
     1681 cctggatgtt ctgaaagtaa agagggctaa tccgacaaaa atacccagca tgatgataac
     1741 cggcaaccgc cattgaggcg gcgggatgaa atagttaacg atttttcctt gcatagagtt
     1801 ttatccagac aattattccg ctaattatta aaacagtacc aaaaataccc aataaaggta
     1861 cgattaatat ataaaagtcg cccacgagcg gtttaaagat ccttccggta tggacctcca
     1921 gggcaaaatt ccaccacgac atcgggcttt tttctataat ctcaagaggc atagccggca
     1981 taacaggttc attgccaagg ctgaccgctc ctttattata atcaaagtaa accgaatttc
     2041 cattttccag tttcaaatac ccgctcacca tttcgtcacc gaagggtttg ctatttgagt
     2101 tggaaggttg tggggggcga ccggagaggt ggttaaatga gactccatct cgaaatgttg
     2161 agactccatc tgggtagaat gagatggggt ctcgagtccc cgggacgcca tccggtggga
     2221 cgccatccct tttcaggtac caatcataaa ttccattgaa tgtgccgaca agatactgac
     2281 cggaacctgt tttctgaaaa acgtttatcc ccatcaccga aagcggcggc tggcccggaa
     2341 ggggaaccat ttccctttcg agactattat cagcaaaaaa caatccttcg gtagtaccta
     2401 aaatatatcc gtctatttct tcatcccaaa tcaccgcccg aagtttatct tcccagggat
     2461 tgggcgtatc catgaccgaa aaaggaatct tcccgacacg ggcattggca atggcaatca
     2521 ataacggcgg acgaagaaac atccctgtta tcgtattaat caaaataaac acagcaatcc
     2581 agatcccggc ttttttgtgc caggttgccg aagccttttt agtggcagag gccagcttca
     2641 gggatttttt tctttttttg agagtcctta gggcataggg cattgcaaaa tgcaaagaac
     2701 ctgtaactgc cagcagaata acgaccaggc cgagggcatc caccaccaac cgtccgatct
     2761 taccataaat ttccccgcta tggatcaccc agatcgtcct gaaaaggccg gtcttattgt
     2821 catagccggc agggggcggc agtttcagtc cgactggatt aaaagacgcg ggattatcaa
     2881 ccgattcaaa catttcagac cgcgtcagga agacgatcct tccctctttt tcaataatcc
     2941 attgtatccg ttcatcctta aatttaaccg gaatcttaac ccatttttcc ttgtcaaaat
     3001 ctaaataaaa taaaccgaat agcgtacctg cataaacatt cccttttgag gagaccagca
     3061 tcgacgatat cttccggttg tcgataccct caggaaatcc ggcattaaaa tctttaaaag
     3121 cggcaaaccg ggtatcagtc agccagatgc cggtattccc aaaaagaagc aggctgtcgt
     3181 ttccgatcat gacacccgat cgtaccgcgg cattgttcca gttgacatac cggttctcct
     3241 tcgggacgag gttcctggtg atatcaaaat tggagaataa ctgcctgtgg ttcatgacga
     3301 caccggagat ggcccagagg atgaaaaaga accccatgat cactccgggc cagcggtgcc
     3361 actttttaaa gaatttcagc catcggttac cgttatgaca gtccttcgga ccggatctca
     3421 tggcttattt cttaaggaaa tagtttataa aatgatcttc agctttctta tccgaccagt
     3481 gatcgaagcc ctgggccaca acccgttcga tcaacggggc cggcaggaaa ggggtaacga
     3541 gttcatagat ctcgccgggt tttaaatcac ccaccccggt aagcacttcg cccagcgggt
     3601 ggccgccctg ttctatgacc ggcctggcat ccaggcgctt cactatttta tcttctatta
     3661 accaggatgg tttcgaggca tttccggagg ctgtcgcttc cagtccttcc aggcggtcga
     3721 ggccaacttt ctgcctgagg gtgttcacca tttcatgcac aggcacctgc ccaacctggg
     3781 ctgcctgctg caaagtggtt accctggcta tcgtccgccg caaaacgggg tttttcagct
     3841 ttttaaagac cggggctatt tcgatcagcg tatcttcgag ttgaggatag gcctccagga
     3901 gttcgtaaac ttttgtgcgt ggggttatga ttagttgttc cattgttcga ttgttcgatt
     3961 gttatttggt tagggatcca aatttccaat ttcaaatttc caattacgac ataaggctac
     4021 tttccatacg ccagtatcct ttgttctcct tcaagtgccc gcttttcggt caggtcctgc
     4081 gatacttcca gggtgccgag gtattcgccg ttttcgtcgc gcaaggcaaa gtactcgatg
     4141 tagatgaact ttcctttcat ctggatccag aagggggcgt ggtcctgccg gccggtcctg
     4201 aaatcttcca ggatttgctc taccacatgt acgctggatg gcgggtggca gagcttgaca
     4261 tcacggttta ggatggcgcg gctgcggtgg aatatgcgct cttttccctg gctgaaatat
     4321 ttcacttttt cgtttttatc gacgaaagtc atatcgaccg gcaaggtatt gagaatagcc
     4381 tgcagttctg ctttggtaaa actgccggaa ggaaggtgga ccaggtcgtt accggcatct
     4441 gcaacctctt cagcggcaat attttcaggt cgccattcta tgtccggatc ataaagacag
     4501 aagccgattt ccagggtttg tttgtagact tcataccatt ccaggtcagt cagtttatcc
     4561 atgaccatcg gtaggagaat ctggtcttct ttcagggtca tatctgcaac tccgtcggaa
     4621 gcgggtttga gaaccatctg cactactgtt tccatctctt ccggagaaag ttgtcccggg
     4681 gcgttcaata cttcgattga agccttcagc aattccctga tttcgttatg tttaccccac
     4741 attaccttag gcggaccggt gattccataa ctttccataa aggggaatac caggttttcc
     4801 ttacggagat aatgcttttc gacgtccatc aggctgttga agagcgtttt aagctggatg
     4861 agaaaagcgg cggtctcttc gtgactctta acggaggaga cccgggtgta aagcgattcg
     4921 agctgatcga ccaccttcag gagctccttg ttttcccttt taaaggtatc gaccggatgg
     4981 ccgggaggga ttattttcat acccgaaagg tcgatggcgc catcgagggc ttcggagtgg
     5041 atatcgcaga atttgagtac ttcggcttcg ctgagggtgc cttcggagat cagttcctgc
     5101 tcgacctcca cgacttcgcc gtaagggatt ttcgtcagga ggctgaccag ttgtttgcgc
     5161 gcttcgtcgg gtgcttcgcc ggcgtggagc tgcaggatca tgtgtttgag cagctctttg
     5221 cgcttttcgg agttgttgat aagttcgctc atatctaatt gcccctaccc ctgtatcccc
     5281 tcccccaatt gggggagggg accaagggga gggggcttgg tttcactctt tgatcattgt
     5341 caacagcatc ttgaactttt ccacagcatt tacagcatga ggaatattgg cgggcatgat
     5401 gatagattgt ccggcgccaa gcaggtaagg aacgcggttg atcaggatct cggcctggcc
     5461 ttccagtacc tgcaccatag cgtcgaaggg tgcggaatgt tcgctgagtt tttgtccttt
     5521 gtcaaaggca aatagggtca cattaccctt ttctttctgg atgacgcgtt tggagacaat
     5581 tccttcggcg gaatattcga ctttatcatt gaagttcatt acctgggagt gtgagaataa
     5641 gggagtttcc atgatattaa ttttaaattt taagttttaa attttgaatt caggtatttt
     5701 tttagtccta agtgtttagt tctaagttcg ggaatatttt tactctgtgt taactctgtg
     5761 tactctgtgg tgaatatcaa atatagcttt ccataagcac ctctcctttg attccgatga
     5821 tggtcttttt tatctgaatt ttccggctgg cggcttcgcg ggccaggttg gcgatatgca
     5881 ccaggccgct gcagcagggc acctccatga ccatcacgtt gagttcctta atattcgctt
     5941 catcgatcat tgttttcagt ttttcgacat agacctcttt tccctggtcg agcttggggc
     6001 aagcgatagc gaggctgcgt cctttcaggt atttctggtg gaaattgccg acggaaaaag
     6061 cgacacaatc ggctgccagc agcacctccg catttcggaa atgaggtgcg gaagggttga
     6121 ttaggtgcat ctggacgggc cattggcgga gctgtgaagg gacggcgtcc ccagggacct
     6181 cgtcccgcgg gacgccatcc ctttcaaaac tctttgctcc cgggcatccg ccatgcgcgc
     6241 aatcaccacc gacactcttc gcatagacct tatccagcac gtcttccagg tcaaatccga
     6301 tgcttccgga attctcctcc agatacccga ctgccgtttc aaaatacatc atttcattgt
     6361 gatcgagcaa atgcttcagg tgcgctacaa cagtgttttc cccgaacttc acgatctctt
     6421 tgatcacagc cacctcgttg taaggctcgg cttcacgttc ctctatttcg atagcgcctt
     6481 ccgggcattc gccgatgcag gcaccgagtc cgtcgcacat caggtcactg accagccgcg
     6541 ctttgccgtc gatgatctgt aaggcccctt cagggcagga ggggatgcaa aggccgcatc
     6601 cattgcattt ttcttcgtcg actttaatga tttcccgttt catattatgg tcattagctc
     6661 gctgcgctcg cgtcattaag tggtcattta ctcacttcgt tcgtgtcatt ggctcgctgc
     6721 gctcgcgtta gtgtttttta ctctgtgtta ctatgtgcaa ctctgtgtaa tttgtggtga
     6781 gtcttaaaac ttccggcaac aaaaatagcc ttcaacttta gaacaggatg taacaaatgt
     6841 tacaacaatt cgagatggca tctcaaattt cactattttt gttttctatt gttagaatga
     6901 taaggttttc cggaaggagc tccatataaa agagatgcca tctcaataag catagaaatg
     6961 tatcaggcac taataaatag cccgatattt accggacttt ctgaagagga actggagaag
     7021 ctctttcaaa ggtgccactg ctttactaaa aactatgaag tgggcgacat agccgctttc
     7081 agcggcgaac cggtgaactt cctgatcatc gttctcgaag ggagtgtgaa aggcgaaatg
     7141 gtcgatttct cgggaaagat cattaagatc gacgatatcc gggcgccata cccgctcgct
     7201 tcggccttta ttttcgggaa acagaacaaa ttcccggtga atgtgatggc gaacgaaagg
     7261 acaaaattgc tttacatacc gaaatacgat ttcatgaaga tgctgcaggc cgatatccgc
     7321 ctgatgcaga actttttgaa tgtgatttcg ggacatacgc agatcctggc aagtaagctt
     7381 aaattcttat
//
LOCUS       DAOQXS010000067         7361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10478_length_7361_cov_3.755817, whole genome shotgun sequence.
ACCESSION   DAOQXS010000067 DAOQXS010000000
VERSION     DAOQXS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_7361_cov_3.755817"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKM34_03150"
     CDS             <1..695
                     /locus_tag="PKM34_03150"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNQ82612.1"
                     /translation="KDYITQISLRDPANTEKYIGSDENWEKAQNAIIEVAKEAGLNYE
                     IVEGEAAFYGPKMDFMVRDALGRKWQLGTIQVDYNLPERFEMEYTGSDNEKHRPIMIH
                     RAPFGSMERFVAVLLEHCAGNFPLWLTPEQVIVLPISEKFTEYGKKVITLLQNSDIRA
                     LIDERSEKAGRKIRDAEIRKIPYMLIVGEKEEEGGTVSVRRHGQGDMGALPVEEFINL
                     VKNEVEESLKHL"
     gene            743..1303
                     /gene="infC"
                     /locus_tag="PKM34_03155"
     CDS             743..1303
                     /gene="infC"
                     /locus_tag="PKM34_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ82613.1"
                     /translation="MATSLPFKGRPPFRDNRAKEEPYKVNELIKAPVVRVVGENIQTG
                     IYNIREALRIAEELELDLVEISPTANPPVCKVVDYKKFLYEKKKKQKEIKANSAKIVI
                     KEIRLSPNIDDHDFNFKLKHAIKFLEEGAKVKVDVFFKGRSIIYKEQGEYVLLKFAAD
                     LEEFGKVEQLPKLEGKRMMMVIAPKK"
     gene            1395..1586
                     /gene="rpmI"
                     /locus_tag="PKM34_03160"
     CDS             1395..1586
                     /gene="rpmI"
                     /locus_tag="PKM34_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ82614.1"
                     /translation="MPKMKSKSGAKKRFAETGTGKIRRKHAYKSHILTKKSNKRKRNL
                     GHFTEVSGADLKNVKDMLQ"
     gene            1662..2006
                     /gene="rplT"
                     /locus_tag="PKM34_03165"
     CDS             1662..2006
                     /gene="rplT"
                     /locus_tag="PKM34_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ82615.1"
                     /translation="MPRSVNAVASKARKKKVLERVKGQWGSRKNVWTVAKNAYEKSLA
                     YAYVGRKQKKRDYRALWIQRINAAVREHDMSYSVFMGKLKEHNIEINRKVLADLAMHN
                     PEAFKAIVDKVK"
     gene            complement(2096..5386)
                     /locus_tag="PKM34_03170"
     CDS             complement(2096..5386)
                     /locus_tag="PKM34_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNQ82616.1"
                     /translation="MSKVLFTLYFSLLIAIAGSGQETGVLTGVLTDQYTGDPLFGVKV
                     RVWEQGNAEAQKSSVFVALSDEDGIFSLELPAGQYQASFSRYGLDKLEIPGIMVLAND
                     TAILNTTLLPVEVAVAGVLVNTEPVYLEIQWTPSWPSIIEKSIDYGPVNDYFVFMNAG
                     SQVAVKFDNNFDHVIGGRIFVGDGSLPGPFLGSDFLLRIYDDDGMGGLPGNTLFEDTV
                     TVDQYGWVGLDSLDAFTSSDHYYLSMYQLNEAPECAPVVSTQYPANSSSLMKPGSYNW
                     MPFPPGNAMIRAWVSAPHDSIHVSSYRVGRFSNFDPNGDPQYGTLTELASTNNCFYND
                     NAFPGLPQGWYAYGIKALYNNGQYSPYRISGIVYNPDPCNPSFTIEQSDGSVSGTCHI
                     SMTGISLPFKNYSFLVQTPGTINLSPIKMGSYSISLYKPGYEKWMIDSVGLFSDTSIN
                     ITLQETIYPIEDLKVNEYTGMLDWENDFVSQYEWQCPADSICHELITPELDLLGLNQW
                     IMTVTYMTPLDNDPPILEYSTDHGRTWALLHEFPLQENWTTVDIDLVVFPNESEGSGI
                     MFRLHDVYGAPYYTDISRIKVWLPSVRCHPEKYYITLDEMPEGQSDSTFYQSDNLENG
                     QSYRAGVFAEYSTGTTDTAFVEFTYHELFPPEDLAWEEENDTLEFSWSVPSGSWNNDK
                     APGDYPGALSGYALTYQTPDNLYRFEIDGPATTSFRFEKPDCDSATAILTAVYDLSDY
                     GYPGSLFESKPAGPLQIKFDGPVENEFFEDWSAISLIHNCWKMTGNGLSIEAGQGFPG
                     AALIYQNPPSHYQVFLTSVPINIPESEDAQRFLECDLTLFAGGYSGYEIMEIHLQQAG
                     SSYWDVVQEFSNSSGSYDWSHYSLDLSGYISSDEFRVRFKFEGIGAEPVQWKVDNIHL
                     HRVCSGPTTLQAELTGENEVSLNWLPNTATKSATSFEHYNIFRKTGQDGFSLLATTTD
                     TFYLDYLKEGGRYSYRVNAAYNDNGILCTSSPSDSAWIVSTYDIPENDIHGRISVYPN
                     PVNERLFIVSDEMIYGINLYNSLGICVLDMDKSGLNFEIDMKSLANGIYFLRIELENK
                     DYNAKIIH"
     gene            5560..6552
                     /locus_tag="PKM34_03175"
     CDS             5560..6552
                     /locus_tag="PKM34_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNQ82617.1"
                     /translation="MKALKIIGIILLILIALFLVVALFLPSKSYMEESIVINKPASLV
                     YKQVNDFRNWEAWSPFQAADPQMVNTYEGAEKGVGATMRWTSKKSGNGFMTITESVPY
                     EKVISTLDFGMPGTTNIFLLKEENGATTVNWAVDIQKMPYPMGRYMAVMMPGMMKPVF
                     KQGLDKMKEVVEAMPDPPALQIVEFPSKAVISVVDSCYWSDIEAKMGQLFGELMAVQQ
                     SSKAQQTGAPLSIYHKWDEESQFTVFENCIPVDREVKSKGRVQYKVLPATRAVMGTHF
                     GPYENTMYLYIALDEYVKDFKLTETGGPIEEYITDPMMEPDTSKWQTNVYFQVQ"
     gene            6563..>7361
                     /locus_tag="PKM34_03180"
     CDS             6563..>7361
                     /locus_tag="PKM34_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ82618.1"
                     /translation="MDRRRFIYKGSGLAAGSLMLNMVPFRLFSGSLKPDLCKVKSGDY
                     YKATRKAIEELGGIGRFIPAGGARVGLLINSAFDEPGAYVHPDIPMAVLYLCWEAGAS
                     EMTLLQPVAEEYWKRSPQFSKHRFIINELKQVESNVFPAVYNEEDFNLMPIIPGAKQL
                     KDVEIIRKIADIDVFINIPILKHHASTILTGALKNMMGLTTRKTNVTFHLGSGKRNDP
                     EYLAQCIADLNLVRKPDLTVADATEFITGNGPAGPGPMKKLDLVVAST"
BASE COUNT         2108 a         1656 c         1816 g         1781 t
ORIGIN      
        1 tcaaggacta tatcacccag atttcgctgc gtgaccccgc caatacggag aaatacatcg
       61 gatcggatga gaactgggaa aaagcgcaga atgccatcat cgaagtggca aaggaggcag
      121 ggctgaatta cgagatcgtc gaaggtgaag ccgctttcta cggccctaag atggacttta
      181 tggttcgcga tgcgttaggg agaaaatggc agttggggac gatccaggtc gactacaacc
      241 tgcccgaacg gttcgagatg gaatacaccg gcagcgacaa cgaaaagcac cgcccgatca
      301 tgatccaccg ggccccattc ggctcgatgg agcggtttgt cgcggtattg ctggagcatt
      361 gtgccgggaa tttcccgctg tggctgacac ccgaacaggt tatcgtactg cctatcagcg
      421 agaaatttac cgaatatgga aaaaaagtta taactttgct gcaaaattcc gatattcgcg
      481 cccttattga tgagcggagt gagaaagcgg gcagaaagat aagggatgcc gagatcagga
      541 aaattccata tatgcttatc gtaggggaaa aagaggaaga aggcggcact gtctctgtgc
      601 gcaggcacgg acagggtgat atgggagctt tgccggttga ggaatttata aatctggtaa
      661 aaaacgaagt ggaagaatca ttgaagcacc tttgatgagg aatcggcttt ataatgttta
      721 actaatttca ggaggatttt ttatagcaac gtcattacca ttcaaaggca ggccaccctt
      781 ccgggacaac agggcgaagg aagaacctta caaggtgaat gaattaatca aagctcccgt
      841 cgtcagggtg gttggagaaa atatccagac cggaatctat aacatccgag aagcattgag
      901 aatagcagaa gagttggagc tcgacctggt ggaaatatcc ccgacggcaa atccaccggt
      961 ctgcaaagtg gttgattata agaagtttct ttatgaaaag aagaagaaac aaaaggagat
     1021 aaaggcaaat tcagccaaga ttgtgatcaa ggagatccgg ctcagcccga acatcgacga
     1081 ccacgatttc aatttcaagc tgaaacatgc tataaaattc ctggaagaag gggctaaagt
     1141 gaaggtggat gttttcttca aaggcagaag cattatctac aaagagcagg gggaatatgt
     1201 gctgctgaaa tttgccgccg acctggaaga atttggaaag gtggaacaac tgccgaagct
     1261 ggaagggaag cgaatgatga tggtgatcgc accgaaaaag tagggatcag agcaattgga
     1321 caatttaaca atagaacaat ttaacaatcg gacaatcgaa caatcgaaca atcgaacaat
     1381 caatatacac aaagatgcca aaaatgaagt caaaatccgg ggcaaagaag aggtttgctg
     1441 agaccggcac cggtaaaatc agaaggaagc atgcgtacaa gagccatatc ctgaccaaga
     1501 agtcgaacaa gcggaaacgc aacctgggac acttcacgga agtatcgggc gctgacttga
     1561 agaacgtaaa agacatgctt cagtaattca atgagtgtta acttactttc agcacggaga
     1621 gtcatgaaac atgggccgct gaagtaaaaa aatgaaaggt tatgcctaga tcagtcaatg
     1681 cagtagcatc caaagcaaga aagaagaaag ttctggaacg ggtaaaaggc cagtggggca
     1741 gccggaagaa tgtctggacc gttgccaaga acgcctatga gaaaagcctg gcttacgctt
     1801 acgtgggccg caagcagaaa aaacgcgact atcgcgccct gtggatccag cggatcaacg
     1861 ccgccgtcag ggagcacgat atgtcgtact cggttttcat gggcaaattg aaggaacaca
     1921 atatcgagat caaccgcaag gtgctggccg acctggccat gcacaacccc gaagccttca
     1981 aggctatcgt ggataaggtg aagtagaggt tggtttgata cgaataaaaa aggctgtccc
     2041 gatctaccgg gcagcctttt tttttccgcc gaccggcgga tgaatctgtc aaaagttaat
     2101 gaatgatctt tgcgttgtaa tctttgtttt ccagttcaat ccggagaaag taaattccat
     2161 tggccagaga tttcatgtcg atttcaaagt tcaaaccgga tttatccata tccaggacgc
     2221 agatgcccaa tgaattgtaa agatttattc catagatcat ttcatctgat acaataaata
     2281 acctctcgtt aaccggatta gggtaaacag agatgcggcc atgtatgtca ttctccggga
     2341 tatcataagt tgagacgatc caggctgagt cggaaggtga ggaggtgcaa aggataccat
     2401 tatcattata cgccgcattg acccggtagc tgtaccttcc gccttccttg agataatcaa
     2461 ggtagaaagt atcggtagtg gttgccagga gcgaaaagcc atcctggccc gtttttcgga
     2521 atatgttgta atgctcaaat gatgtggctg atttggtcgc tgtattcggg agccagttca
     2581 ggctgacttc attctcaccg gtcaattcag cctgaagagt tgttggtccg gaacaaactc
     2641 tgtgtaaatg aatattgtcc actttccact gtaccggttc ggctccaatc ccttcaaatt
     2701 taaaccggac cctgaattca tccgagctga tatatcctga taagtccaat gaataatggc
     2761 tccagtcgta acttcccgat gaattggaga attcctgcac cacatcccaa taggaggacc
     2821 cggcctgttg cagatgaatt tccatgatct catagccgct gtagcctccg gcaaagaggg
     2881 tgagatcgca ttcgaggaaa cgttgcgcat cttcgctttc aggaatattt atcggcacgc
     2941 tggtcagaaa aacctggtag tgggaaggtg ggttctgata gataagtgca gctcccggaa
     3001 agccctgccc ggcttcaatc gacaggccgt ttccggtcat tttccagcag ttatggataa
     3061 ggctgatggc cgaccagtcc tcaaagaatt cattttcaac cgggccgtcg aattttattt
     3121 gcagcgggcc ggcaggtttc gattcaaaca ggcttccggg ataaccatag tctgataaat
     3181 catatacagc cgtaagaatt gctgtggcag aatcgcagtc cggtttttca aagcggaagg
     3241 aagtcgtcgc agggccgtcg atctcaaacc ggtacaagtt atccggtgtc tggtaagtca
     3301 gagcatagcc gctaagcgca cctggataat cgcccggtgc cttgtcgtta ttccaggacc
     3361 cggatggtac gctccaggag aattccaggg tatcattttc ttcttcccag gccaggtctt
     3421 ccggtggaaa aagttcatgg taggtgaatt ccacaaacgc cgtatcggtg gtaccggtgg
     3481 agtattcagc aaatacccct gcacggtaac tttgcccgtt ttccaggttg tcagactgat
     3541 aaaatgttga atcggattgc ccttccggca tttcatccag ggtgatataa tacttttcag
     3601 gatgacacct gacacttggc agccaaactt ttatccggct gatatccgtg taatatggag
     3661 cgccataaac atcatgcaac cggaacatga ttcctgatcc ttcggattca ttcgggaaaa
     3721 caacgaggtc aatgtcaaca gtagtccagt tttcctgcag aggaaactca tgcaggagag
     3781 cccaggtccg gccatggtcg gtcgaatatt ccagaattgg gggatcgttg tcaagaggag
     3841 tcatgtatgt taccgtcatg atccattgat taagaccgag taggtcaagc tccggggtga
     3901 tcaactcgtg gcaaattgaa tcggctggac attgccattc atattgactg acaaaatcgt
     3961 tctcccaatc gagcatgcct gtgtattcat tgactttcag gtcttcaatt gggtagatcg
     4021 tttcctgtaa tgttatattg attgaggtgt cggaaaataa ccccacggaa tctatcatcc
     4081 atttctcata acccggttta tataatgaaa tactataact tcccattttt atcggtgata
     4141 aattgattgt accgggagtc tgcactaaaa aagaataatt cttaaaagga agggatatgc
     4201 cggtcattga gatatgacaa gtgccactta ctgaaccatc agattgttct atagtaaaag
     4261 aagggttgca tggatctgga ttataaacga tacccgaaat tctatatggc gaatattgcc
     4321 cattgttata caatgcttta attccatagg cataccagcc ttgcggaaga cccggaaagg
     4381 cattatcatt ataaaaacaa ttattggttg aggccagttc agtcagagtt ccatattgcg
     4441 gatcaccatt tggatcgaaa ttagaaaagc gtcctactct gtaacttgat acgtgaatgc
     4501 tgtcatgcgg agctgaaacc catgcccgga tcatggcatt tcccggggga aaaggcatcc
     4561 agttgtagct tcccggtttc atcaagctgc tgctgttagc aggatactga gtgctgacaa
     4621 ccggcgcgca ttccggcgct tcgttcaact ggtacatcga caggtaataa tgatccgacg
     4681 aagtaaaggc atccaggcta tccaatccaa cccagccata ctggtcaacc gtcacagtgt
     4741 cttcgaacaa ggtgttgccc ggaagtcccc ccattccatc atcatcatat attctcagca
     4801 gaaaatctga ccccaggaat gggccgggga gtgagccatc acccacaaaa attcgccctc
     4861 cgattacatg atcgaaatta ttgtcgaatt taactgccac ctgactacct gcattcatga
     4921 aaacgaagta gtcatttaca gggccataat ctattgattt ttctattatt gacggccagg
     4981 agggcgtcca ttggatttct aaataaacag gttcagtatt aacaaggacg ccagctactg
     5041 ccacctcaac cgggagcaag gttgtgttaa gtatggccgt atcattcgct aacaccatga
     5101 taccaggaat ctcaagttta tcaagcccat accggctgaa cgatgcctgg tactgaccgg
     5161 ccggcaattc caggctgaaa atgccgtcct catccgacaa agccacaaaa accgaactct
     5221 tttgcgcttc tgcatttccc tgctcccaga cccgcacttt cactccaaac aaaggatcac
     5281 cggtgtattg atccgttaaa acgcctgtca ggaccccggt ttcctgacct gagcctgcaa
     5341 tggcaatcag caagctaaag taaagtgtaa ataagacctt tgacatggca tttgaacttt
     5401 tggtttatag taataattat aagacatata tatcattgaa acagttgcat agaccaaatt
     5461 ttaattcctt gacacattga cagtatatca ggataccagt tattttttaa ttttgacaag
     5521 gataactcga tattgtaatc atctataatt aaaaccatta tgaaagcatt aaaaatcatc
     5581 ggaattattt tgctcatact catcgcgctg tttctcgtgg tagccctgtt ccttccttcc
     5641 aaatcttaca tggaagaatc gattgtgatc aacaaaccgg ccagcctggt ttacaagcag
     5701 gtgaacgatt tccggaactg ggaggcctgg tcgccattcc aggcagccga tccccaaatg
     5761 gtcaatactt atgaaggtgc ggagaaaggc gttggcgcta cgatgcggtg gaccagcaaa
     5821 aagagcggta atggatttat gaccatcact gagagtgttc cgtatgagaa agtgatatcg
     5881 acgctcgact tcggtatgcc gggaacaacc aacattttcc tgcttaagga agagaacggc
     5941 gcgaccactg taaactgggc cgtggacatt cagaaaatgc cttacccgat ggggcgttat
     6001 atggccgtta tgatgcccgg tatgatgaaa ccggtattta aacaaggcct cgataaaatg
     6061 aaagaggtag tcgaagccat gcccgacccg cccgctttgc aaatagttga atttccttcc
     6121 aaagccgtta tttctgtggt cgattcctgc tactggtcgg atattgaagc aaaaatgggc
     6181 cagttgttcg gcgagctgat ggctgttcaa caaagttcaa aagctcaaca gacgggtgca
     6241 ccgctctcga tataccataa atgggacgaa gaaagccagt tcaccgtttt tgagaactgc
     6301 atcccggtcg accgcgaggt gaaatccaaa ggccgtgtcc aatacaaggt gctgccggct
     6361 acccgggcag ttatgggtac ccatttcggc ccatatgaga atacgatgta cctgtatatt
     6421 gctttggatg aatatgttaa ggatttcaag ctgactgaga cgggaggtcc gattgaggaa
     6481 tacattaccg atcccatgat ggaacccgat accagcaaat ggcagacaaa cgtttatttc
     6541 caggtacagt aaaatcacat ctatggaccg gaggcggttt atttacaagg gatcggggct
     6601 ggctgccgga agcctgatgc tcaacatggt tcctttcagg ttattttccg gctcattgaa
     6661 gcccgacctc tgcaaagtga agtctggcga ctattacaaa gccacccgca aagcaattga
     6721 agagctcggt ggaatcggcc ggttcatccc ggctggtggg gccagggtcg gactgctgat
     6781 caattctgcg ttcgatgagc ccggtgctta tgttcacccg gatatcccga tggccgtact
     6841 ttatctctgc tgggaggccg gggcatcgga aatgacgctg ctccagccgg ttgcagaaga
     6901 gtactggaag aggagcccgc agttcagcaa gcacagattt atcatcaatg aattaaaaca
     6961 ggttgaaagc aatgtttttc cggctgttta taatgaggaa gattttaact tgatgccgat
     7021 aattccaggt gcgaaacaac tgaaagatgt cgagatcatc cggaagatcg ctgatattga
     7081 tgtgtttatc aatattccaa tccttaagca tcatgcttcg acgatcctga ccggagcgct
     7141 gaaaaacatg atgggcctga cgacgcgcaa gaccaatgtg acattccacc tgggatcggg
     7201 caagaggaat gacccggagt acctggccca gtgtattgcc gacctcaacc tggtccgcaa
     7261 gccggatctg actgtggccg acgccaccga attcatcacg ggcaacggtc cggccggccc
     7321 ggggccgatg aaaaagctgg acctggtagt cgcttcaacc g
//
LOCUS       DAOQXS010000068         7350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10501_length_7350_cov_3.559836, whole genome shotgun sequence.
ACCESSION   DAOQXS010000068 DAOQXS010000000
VERSION     DAOQXS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10501_length_7350_cov_3.559836"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1316)
                     /locus_tag="PKM34_03185"
     CDS             complement(264..1316)
                     /locus_tag="PKM34_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82619.1"
                     /translation="MLELRNISKSYPDFKLHDISFRVEDGDYFIILGPSGAGKTQILE
                     IIAGLVHPDEGGMFIDERDITEERIQSRGIGIVFQDYAVFPHMKVRENLAYSLRRTGY
                     EKGQIARLVAEQAEAMEISHLLDRRPSTLSGGELQRVALARTLIRKPRYLLLDEPLAS
                     LDVSLRQGLRSLLKQLNQAGQTIVQVTHDYEEALVLADKVAVIHQGKIIQSGKADDVF
                     HHPGSEFVARFTGIRNYFPARLFREEDRQYGELENGAILRLATDETEGRGFILIRGED
                     IFISLEDLPTSATNRFPGRIAGISPSVYGFEVLVDTGTLYYVRITRESLQNLGLHEGK
                     EVWISFKASNVRFVRV"
     gene            complement(1309..2106)
                     /locus_tag="PKM34_03190"
     CDS             complement(1309..2106)
                     /locus_tag="PKM34_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82620.1"
                     /translation="MYFRFIMRFQPFQLFLILLSGVVLLFIIGPLLGMFISTTPYQLF
                     ETSKDPEVQRSIGLTLWVSMAATLVFALGGIPLAYLLARKDFALKKVILGIIDLPIVI
                     PHSAAGIAVLGFISRETVVGKMGSAIGLDFVGAPAGIAIAMGFVSIPFLVNAARDGFL
                     AVPVRYEQAALTLGASPIRVFFTISLPLAWRNIVSGLILMFARGMSEFGAVIIIAYHP
                     MVAPVLIWERFSSFGLSYARPVAVLFILVSLVFFIGLRFLSRTKKDA"
     gene            2247..2963
                     /locus_tag="PKM34_03195"
     CDS             2247..2963
                     /locus_tag="PKM34_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ82621.1"
                     /translation="MGWLYDLMSKDVRFVEGLKACINCGTCTAICPAAEFYNYDPRIL
                     VTTLQTRDDEKIEALLKSDFIWYCGECMSCKTRCPRGNVPGGLISVCRSFSQDLGYFI
                     ESEKGRQQLAVKRTIGEWILDHGYCLFPQQIEPESHPEQGPVWEWITNSLKEVYGRFD
                     ATYQEEGPGILRKIPQDSLDELKRIFDVTGGTERFEKLEEYSKKKAEEMGLEFSPGQK
                     SEYYLHVYNFNSNKHGKDEH"
     gene            2953..4026
                     /locus_tag="PKM34_03200"
     CDS             2953..4026
                     /locus_tag="PKM34_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNQ82622.1"
                     /translation="MNIEGKKIIWSQYQKEIADNDYFYARSCVRQSFFPGSETTFMKI
                     VRDDLGKNISEHPFHTTCTGIAYHSDVVPFETTMTVIARQFALMTESGFQNYVPSCIT
                     SFGMYNEVLETWHHFPEVEMKVRKMLKEATGKEFEKPKNLVHSSDIIYKFRKEIAEKG
                     KYRLINRFTGEPIRVVEHIGCHYSKMFPSKGVGGAEYPHVLVGMIEEWGGEVVDYPER
                     RHCCGFGFRQYVVKANRGYSIACSKKKFESMQPYRPEAILTNCPGCPFFFDRWQYAIS
                     EMEGKTYGENGQGIPVLTYEELAGIILGYDPWDLGFQTHQVPVEPLLDKIGIPYDPEK
                     KFLGKNGEFIGRPDMPDNLKFCH"
     gene            4030..5052
                     /locus_tag="PKM34_03205"
     CDS             4030..5052
                     /locus_tag="PKM34_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ82623.1"
                     /translation="MTTKVVAVIGGGAAGLEAAANLAGFGYTVHLIEKEHQPGGHVKE
                     WDRLFPSRRRASEIIGHLGGQLNENVRIHLGKEIINIRRDNGSWRIGLNDESRLDAQA
                     VILATGFDLFEAAKKEEYGYGIYDNVITSADLERMFREKGEIRNHQGMIPQRVGFVHC
                     VGSRDEKVGNLYCSRVCCVTAVKQAIEVCEMIPGCEVFCFYMDLRMFGLDFEALYKEA
                     QEKWGIHFIRGRLSEAFENPDGSVLVKVEDTLAARPLKMKTDLLVLMSGMVPNAGTKR
                     LVEMLGLQTDENRFLLPADRQLRANETGIPGIFVAGTCTSPKSIDETLADARAAAIKV
                     MEYFRE"
     gene            5109..5444
                     /locus_tag="PKM34_03210"
     CDS             5109..5444
                     /locus_tag="PKM34_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ82624.1"
                     /translation="MKNFGYAINSDRQIDYDSNDHRVARYLAEHEPSFMVCFACGTCS
                     ATCSAGLFTDLSLREMIGNIKRGEISKIKSEAEKCMLCGKCQLACPRGVNTRNVILTI
                     QRALVKYNL"
     gene            5441..7255
                     /locus_tag="PKM34_03215"
     CDS             5441..7255
                     /locus_tag="PKM34_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNQ82625.1"
                     /translation="MTFDFFVLPFFLGLITLLVILAVKYSRWIREFDPADKALIKKNI
                     FTTKSLRAAGEVFMESLLHRKMFRKNAMLGYMHMSFAMGWFLLIVCGNLESRIYSKVH
                     LNPPYYPIFLKFFVHDHRVLAHELLSIPGVFRVLMDAILLMILSGLALALFKRASSRW
                     FGMRKASKHSLYDRIAITTLWLIFPLRLLAESFTAATYEGGSFLTNSFGSFLGSFLPA
                     ETLAYPAWWAYSLALGGFFVSLPYSRFMHIPTEIMLIFFRHYGVKPSLHFNHYSNFEI
                     QSCPKCGVCIDACPVHTDANRDGIPPAYFLRSLRDRQPMPSSTFDCLLCGRCESYCPV
                     GININAMRLSQRVEFSAHLNGSFRYLNGATARPASVAYFAGCMTHLTPSIKKSMIMIF
                     KEGGVEFNFIDEEGSVCCGRPLWMAGKEKQARELMEHNKKLIESTGCTTLVLSCPICL
                     KVFREEYGMKMEILHHSQFILRMIDQGKLRLSKHGLRVAFHDPCELGRGSGIYEEPRK
                     VIRNFAELVAAGEERNMSVCCGGSLGSTGIDAERRDGVSHQALETLMQNRPDAIVTAC
                     PLCKKTLQKDSPVVVSDISELTVLALETKKIEAAATMN"
BASE COUNT         1876 a         1818 c         1982 g         1674 t
ORIGIN      
        1 tggagtctca aagaattacc cgattcggca ttgtaacagg gtttccaacc tttttgctcc
       61 acacaatcga atgaattata gaaagttata gagatgctta aatattgtta taaattatcg
      121 gatatttaaa aatcacataa tcagtatgtt acaatttata taacgatttc caattaatag
      181 ggttggaaac cctgtttcta caactgtcca actgcgatcc ttcgtccgcc aaaggcggat
      241 tcaggacatg ctgtccaact gacctatacc cgcacaaacc tcacgttgct ggctttaaag
      301 ctgatccaca cctctttgcc ttcgtgcagg ccgagattct gcaacgattc gcgggtgatt
      361 cgcacgtagt aaagcgttcc ggtatcgaca agcacctcga agccgtagac cgacggactg
      421 atccccgcaa tacggcccgg aaaccggttg gtggcgctgg tcggcagatc ttccagcgaa
      481 ataaaaatat cttctccccg gataaggata aacccgcgcc cttccgtctc atcggtcgcc
      541 agccgcaaaa tggcgccgtt ctccagctcg ccgtactgcc ggtcttcttc cctgaacagc
      601 cgtgccggga aatagttgcg gatgccggtg aaacgggcga caaactccga accgggatgg
      661 tgaaacacat catcggcctt tcccgactgt atgatcttac cctggtggat cacggcaact
      721 ttatcggcca ggacaagcgc ctcttcataa tcatgggtta cctgcacgat cgtctgcccg
      781 gcctggttca gttgcttcag caggcttctc aaaccctggc gcagcgaaac atcgagcgat
      841 gccagcggct catcaagaag caaataacgc ggcttccgga tcagggtgcg ggccagcgcc
      901 acgcgttgca gttcgccacc cgagagggtc gacggccggc ggtcgagcaa atgactgatc
      961 tccatggcct ccgcctgctc cgccaccaac cgggcgatct gacctttttc gtaaccggtg
     1021 cggcgcaacg aataggccag gttctccctg actttcatgt gggggaaaac ggcatagtcc
     1081 tggaatacaa tccctatccc gcgcgactgg atcctttctt ccgtaatatc ccgctcatcg
     1141 ataaacatcc ctccttcgtc gggatgaaca aggccggcga tgatctcgag gatctgcgtc
     1201 ttgccggcac ccgacgggcc caggatgatg aaataatcac catcttccac ccggaaggag
     1261 atatcatgca gcttgaaatc aggatatgat tttgatatgt tccttagctc aagcatcttt
     1321 ttttgtcctc gataaaaatc ttaatccgat aaaaaacaca aggctgacaa ggataaacaa
     1381 gactgctacc ggccgggcat aactcagccc gaacgagctg aacctttccc agatcaatac
     1441 cggcgctacc atcgggtggt aagcaataat aatcacggcg ccgaattcgc tcatcccgcg
     1501 ggcgaacatc aggatcagcc cggagacgat attgcgccag gccagcggta gcgagatggt
     1561 gaagaacacc cggataggag atgcccccag ggtcagcgcc gcctgctcgt aacgtaccgg
     1621 cacggccagg aatccgtcgc gtgcggcgtt gaccaggaac ggtatgctga caaatcccat
     1681 cgctatggca atgcctgccg gcgcaccgac aaagtcgagg ccgatagctg aacccatttt
     1741 cccgacgacc gtttcgcgcg agatgaagcc cagcaccgca atacccgcgg cggaatgggg
     1801 gataacgatc ggcaggtcga tgatccccag gatgactttt ttgagggcaa aatcctttct
     1861 tgccagaagg taagccagcg gaatcccgcc cagggcgaat accagcgtcg ccgccattga
     1921 tacccataat gtcagcccga tgctcctttg cacctcggga tctttcgatg tctcaaaaag
     1981 ctgatagggt gtcgtcgaaa taaacatccc cagcagcggc ccgatgatga ataaaagaac
     2041 cacgccgctg agcaggatca gaaatagctg aaaaggctga aaccgcatga tgaaacgaaa
     2101 gtacaaattt ttccgaaccg aagggacgct cgccggacta ccttatattt tggcaaaaaa
     2161 tgtatggcta aatgaatatg cattaatttc atcattcaat tttactacat ttgttaaaga
     2221 attaacaata gtttaagtct gtctttatgg ggtggttata cgatctgatg agcaaagatg
     2281 tcaggtttgt cgaaggcctg aaagcctgca tcaattgcgg aacctgcaca gccatctgcc
     2341 cggcggcgga attttataac tacgatccgc gcatcctggt caccaccctt caaacccgcg
     2401 acgacgaaaa gatagaagcc ctgctgaaaa gcgatttcat ctggtattgc ggcgaatgca
     2461 tgtcgtgcaa aacccgctgt ccacgcggga atgtcccggg aggactgatc tcggtttgcc
     2521 ggtcgttttc gcaagacctg ggctacttta ttgagtccga gaaaggaagg cagcaactgg
     2581 ccgtaaaacg taccatcggc gagtggatac tggatcatgg ctattgcctg ttcccgcagc
     2641 agatcgaacc cgaatctcat cccgaacagg ggccggtatg ggaatggatc accaacagcc
     2701 tgaaagaggt ctatggccgg ttcgatgcca cttaccagga ggaaggaccc gggattttac
     2761 gcaagatccc gcaggattcg ctggatgaac tgaaacggat cttcgatgtg accggtggga
     2821 ctgaacgatt tgaaaagctg gaggaatatt cgaagaagaa agccgaagag atgggacttg
     2881 agttcagccc gggacagaaa agcgaatact atcttcacgt atataatttt aacagtaata
     2941 agcacggcaa ggatgaacat tgaggggaaa aaaataatct ggagccagta ccagaaggag
     3001 attgccgata acgattattt ctatgcccga agctgcgtga ggcagagctt tttcccgggt
     3061 tcggaaacga cttttatgaa gattgtaagg gatgacctgg gtaaaaatat ctcagagcat
     3121 ccattccata cgacttgcac agggatcgcc taccattctg atgtcgttcc atttgaaact
     3181 acaatgacgg tcatcgcccg ccagttcgct cttatgaccg aatcgggctt tcagaattat
     3241 gtcccttcct gtataacgtc attcggaatg tataacgagg ttctggaaac atggcaccat
     3301 ttccccgagg tggagatgaa agtgcggaag atgctgaaag aggccacggg gaaagagttt
     3361 gagaaaccga agaacctggt ccattccagc gatatcatat acaaattccg gaaagagatc
     3421 gcagaaaaag gaaaataccg gctcattaac cgctttaccg gtgaaccgat ccgcgtggtg
     3481 gagcacatcg gatgccacta ttcgaagatg ttcccgagca aaggggttgg cggggcggaa
     3541 tatccgcatg tccttgtcgg gatgatcgaa gagtggggag gagaagtggt cgactacccg
     3601 gaacgccgcc attgttgcgg ctttggattt cgccagtacg tggtcaaagc caaccgtggc
     3661 tattccattg cctgctctaa gaagaaattt gaatcgatgc aaccctaccg gcccgaagcg
     3721 atcctgacca actgcccggg ctgcccgttc ttcttcgacc gctggcagta tgccatcagc
     3781 gaaatggaag ggaaaaccta tggcgaaaac gggcagggga tacctgtcct tacctacgaa
     3841 gaactggccg ggatcatcct gggttatgat ccctgggacc tgggctttca gacgcaccag
     3901 gtgcccgtgg aaccgttact ggataaaatt ggaattcctt atgatcccga aaagaaattt
     3961 cttggaaaaa acggtgaatt tattggtaga ccggacatgc cggataatct caaattttgc
     4021 cattgaccta tgaccacgaa agtagttgct gtaatcgggg gtggcgccgc aggactggag
     4081 gcggcggcaa atcttgcagg gttcggctat accgttcatt taatagaaaa agaacatcag
     4141 ccaggcggtc acgtcaaaga atgggaccgg ctgtttcctt ctcggcgccg ggcatctgag
     4201 atcatcgggc atctcggagg gcaactcaac gaaaatgtca ggatccacct ggggaaagag
     4261 atcataaata taagaaggga taacggatct tggaggatcg gactgaacga tgaatcgcga
     4321 ttggatgctc aagctgtgat cctggccact gggttcgacc tgttcgaagc cgcgaaaaag
     4381 gaagaatacg gttatgggat ttacgacaat gtcatcactt cggccgacct ggagagaatg
     4441 ttcagggaga aaggggagat caggaatcat caggggatga ttccgcaacg ggtgggattt
     4501 gtccattgcg tgggatccag ggatgaaaag gttggcaatc tttattgctc ccgggtttgc
     4561 tgtgtgacgg ccgtcaaaca ggccatcgag gtctgtgaaa tgatccccgg ttgcgaagtg
     4621 ttttgttttt atatggacct gaggatgttc ggactcgatt tcgaagcgct ttacaaagaa
     4681 gcccaggaga aatggggcat ccactttatc cgggggaggc tttcagaggc tttcgagaat
     4741 cccgacggat cggtgctcgt aaaggtagaa gacaccctgg ccgcgcgccc gttgaaaatg
     4801 aaaacggacc tgctggtgct gatgtcgggc atggtgccca atgccggcac aaaacggctg
     4861 gtggagatgc tgggcctgca gacggatgaa aaccgtttcc tgctgcccgc cgatcgccag
     4921 ctccgggcca acgagaccgg tatccccggt attttcgtcg ccggcacctg cacctctccg
     4981 aagagtatcg acgaaacgct cgccgatgcc cgggcggcag cgatcaaagt gatggagtat
     5041 ttcagggaat agtgaatagc gctctgatac atggacaatg gacaatggac aatggacaat
     5101 agtgaatagt gaaaaatttc ggttatgcga taaattctga ccggcagatc gattacgaca
     5161 gtaacgatca ccgggttgcc aggtacctgg cggagcatga gccgtcgttc atggtctgtt
     5221 ttgcctgcgg cacctgctcg gcgacctgca gcgccggatt gtttaccgat ctcagcctac
     5281 gggagatgat cgggaatatt aaaagaggtg agataagtaa aattaaatcc gaagcggaga
     5341 agtgcatgct gtgcggaaaa tgccagcttg cctgcccgcg gggagtcaat accagaaatg
     5401 tgatcctgac tattcagaga gctttggtga aatataattt atgacgttcg acttctttgt
     5461 cctgccgttt ttcctgggcc tgatcaccct gctggtgata ctggcggtga aatattcccg
     5521 ctggatcagg gaatttgacc cggcagacaa agcgctcatt aagaaaaaca ttttcacgac
     5581 aaaaagcctc cgggcggcag gagaggtatt catggagagc ctgctgcacc ggaagatgtt
     5641 ccggaagaat gccatgctgg gctatatgca catgagcttt gccatgggct ggttcctgct
     5701 gatcgtttgc gggaacctgg aatcgagaat atacagcaaa gtccacctga atccccctta
     5761 ttatccgata tttctgaaat ttttcgtgca cgaccaccgg gtgctggccc atgagctgtt
     5821 atccataccg ggcgttttca gagttttgat ggatgccatt ttgcttatga tcctgtcggg
     5881 gctggcgctg gcgctgttca aaagggcgtc atcacgctgg ttcggaatgc gaaaagccag
     5941 taaacacagc ctgtatgacc ggatcgccat aaccacgctc tggctgatct ttccgcttcg
     6001 cctgctggca gagagcttca cagctgcaac ttatgaagga gggagcttcc tgaccaacag
     6061 tttcggcagt ttcctgggaa gttttctgcc ggccgaaaca ctggcctacc cggcctggtg
     6121 ggcctattca ctggctctgg gcggattctt tgtcagcctg ccttattccc ggtttatgca
     6181 cattcctacg gagatcatgc tgatcttttt caggcactac ggtgtgaaac catcgctgca
     6241 tttcaaccat tattcgaatt tcgagatcca gtcgtgcccg aagtgtggtg tgtgcatcga
     6301 tgcatgtccg gtgcataccg atgcgaaccg ggatggcatc cccccggcct atttcctccg
     6361 ttcgctccgt gaccggcaac cgatgccgtc gtccactttc gattgccttc tctgtggccg
     6421 ttgcgaatcg tactgcccgg taggcataaa tatcaacgcc atgcgccttt cgcaacgggt
     6481 ggagttctcg gctcatctca acggttcgtt ccgctacctg aacggcgcaa cggccagacc
     6541 ggcctctgta gcctattttg cgggttgtat gactcatctg acaccgtcca taaaaaagtc
     6601 gatgataatg atatttaagg aaggcggtgt cgaatttaat tttattgatg aagagggaag
     6661 cgtatgttgc ggaaggccac tgtggatggc cggcaaagaa aaacaggcgc gtgagctgat
     6721 ggagcacaat aaaaaactga tcgaaagcac aggttgcacc acgcttgtcc tttcgtgccc
     6781 gatttgcctg aaagttttcc gggaggagta tgggatgaag atggagattc tgcatcattc
     6841 gcagttcata ctcaggatga tagatcaagg aaagctgaga ttgtcgaagc acggcttgcg
     6901 ggtggcattc catgacccct gtgaattggg gagaggttcg gggatatatg aggaacccag
     6961 gaaagtgatc cgaaattttg ccgaactggt tgcagccggc gaagagcgga acatgtcggt
     7021 ctgctgtgga ggcagcctgg gaagcactgg aatcgacgct gaacggcggg atggggtatc
     7081 gcaccaggcc ctggaaacat tgatgcagaa caggcctgat gccattgtaa ccgcctgtcc
     7141 gctatgcaaa aagaccctgc aaaaagattc accggtggtg gtttccgaca tcagtgaatt
     7201 aacggtgctg gctttggaaa caaaaaaaat tgaagctgct gcaacaatga attgaaaaat
     7261 atttacattt gcactccttt tttgacgtat tgcggaagta gctcagctgg tagagcacga
     7321 ccttgccaag gtcggggtcg cgggttcgaa
//
LOCUS       DAOQXS010000069         7341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10518_length_7341_cov_3.131622, whole genome shotgun sequence.
ACCESSION   DAOQXS010000069 DAOQXS010000000
VERSION     DAOQXS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10518_length_7341_cov_3.131622"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /locus_tag="PKM34_03220"
     CDS             complement(<1..2050)
                     /locus_tag="PKM34_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ82626.1"
                     /translation="MNKRYVFLIVTLLLSFRALAQMFILNEDFNGASGTVPPQGWSNL
                     VIAGSADDKWHFDNPGDRLLNYPVTEPFAVFDGDSVSNDGSPEEIALESPLFDASTSN
                     YILLNFVHVFDPGSGATIRIDAYNGSSWVGVTELQPTESNPTSAIVDLSDATGGITNA
                     RIRFIWSGNGSGFWAIDNIRIYASLPLDGGVVTVDSPESPVTPGIQDVRISLGNFGYN
                     TLTSTTINWTADDVAQPAHTWNGSIGFGQTGNNITIGSYNFTKPVMIKVWHTQPNGQN
                     DLNPYNDTVSEYLVPPLCGTYTIGGNEADFESFSQIADVLNIAGITCPVTIIVRNGTY
                     FDQFEIRQIQGSSAVNTVTFESESGDSTQAVIRIIPNALKYEPMILLNGAENISFRHL
                     GLSTGSNSSQNDALVIRNCSEIEVANCALTSRNEADYGIVIDGGSRNIGVRNNYFECI
                     FGRSGAVNISGSGTRYATITGNYIKGATEKGYSTIRIEEAASRITVSDNRIERCFRAV
                     HIIQSDSILISGNHINNTNDGIYTEQSSSRIFITSNRLTHIKSNQNVPEGNSGIFMKN
                     SSYAEISNNYIHTTGGGTVMGVWLQSVNSSRVCFNSVNITNSETQGKSNGILIEGNNS
                     LMARNNIFRVKNSGTPVKVTGSVSQLDFDHNDYYSSGPVIGKLGENEYESITSWNLAT
                     G"
     gene            complement(2188..2811)
                     /locus_tag="PKM34_03225"
     CDS             complement(2188..2811)
                     /locus_tag="PKM34_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNQ82627.1"
                     /translation="MTSGYYYRILLIILLNALLFKGFGQEPPWDFIKEKEGIRIYTRS
                     EETSSVKSFKGEADFLTGMDAISRIIGRVDKFDWWDDNISEIKVLEYVEEKLIRYYLV
                     YDVPWPLSDRDLCVEAIITNDPVTGKRIVHAKPISGLVPEYSDRVRITNYWQRWTMED
                     AGNGLVHLVLEGSVDPGGNIPSWLINMVITDTPLNIMRKVREQVEKN"
     gene            2908..3762
                     /gene="deoC"
                     /locus_tag="PKM34_03230"
     CDS             2908..3762
                     /gene="deoC"
                     /locus_tag="PKM34_03230"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ82628.1"
                     /translation="MMNQIFKEYRFTEKELAARADGITEKILPDETDIVLISRLLGCI
                     DLTTLEGSDTQRKVTDLCRKAQFSAISERFPEAAAVCVYPSLVKTAVDELKGTAIRVA
                     SVAGGFPSGQTGLRIKLEEVKFAVNEGADEIDTVISRGKLIGGDETEVFDELCAIREM
                     CGPVHMKVILETGELPSVGYVRRASELAIAAGADFIKTSTGKISEGATAVPFLVMLDT
                     IREYHDLTGKTIGIKPAGGIRTFGNATVFTALLIEILGDTWFCKESFRIGASSLADDL
                     VDKGLRLV"
     gene            3817..4227
                     /locus_tag="PKM34_03235"
     CDS             3817..4227
                     /locus_tag="PKM34_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82629.1"
                     /translation="MKVQLSLLALLALMLFFPACLPEDEDIDTGDPVEKFLGTWSVNE
                     SCRRGNYSLEITRDPGNSAQVLVYNFGNPGPGYDPAVGLVVSNNLYIQSQTIGEGWTV
                     DGKGTYTSNGSISWDYSLVIPPNELNCTADSHRQ"
     gene            complement(4228..4632)
                     /locus_tag="PKM34_03240"
     CDS             complement(4228..4632)
                     /locus_tag="PKM34_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNQ82630.1"
                     /translation="MHRIFSAIIAISFLVSCYHTAPGPEFNRSLLIPADSMAIIITDL
                     HLADGAINARKVHKDSLKTVSSAYLNEILTRHNVTKEQFDESMRFYTYDPREISKIYE
                     QVIIELGKKESQVMPRKEERKEEVKSEKKTVE"
     gene            complement(4632..6830)
                     /gene="recQ"
                     /locus_tag="PKM34_03245"
     CDS             complement(4632..6830)
                     /gene="recQ"
                     /locus_tag="PKM34_03245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNQ82631.1"
                     /translation="MIVKEALSMKEVLKDVFGFDNFKGNQEIIIKHLLSRKDTFIIMP
                     TGGGKSLCYQLPAMMCEGTAIIVSPLIALMKNQVDNLRNYGTDHNLAHFMNSSLSKSE
                     MKEVRQDLVEGYTKLLYVAPESLTKEDNVEFLRNINISFYAIDEAHCISEWGHDFRPE
                     YRRIRPIIETIGQDVPVIALTATATPKVQQDIIKNLEIPQSTIFKSSFDRPNLYYEIR
                     PKTENVAKDVIKYIKANLGKSGIVYCMSRKKVEEMAETLVVNGIKALPYHAGLESNTR
                     KSNQDRFLMEDVDVIVATIAFGMGIDKPDIRYVIHYDMPKSLESYYQETGRCGRDDGE
                     GNCIAFYSYDDILKLEKFMKDKPVAEQEIAKELLQETVAYAESAVCRHKLLLHYFGEE
                     FPKENCGSCDNCLHPKAKFEGKDYIKLVIETVLAVKQQFKAKHIVNIIIGKNSTTMKS
                     YKHNVLEEFGKGADEDERFWNAAVRQALISQLLVKDIENYGILKISKEGHKFLKKPYS
                     IMLTHDRDYDNLEEEEDIMEGAHKTSTADKNLFAMLKDLRKEISRKENLPPFVIFQDP
                     SLEDMAIQYPITSEELLQITGVGTGKAHKYGDPFLKLIQQYVEENDIIRPNDMVVKSV
                     VNKSGLKVYIIHNIDRKLALEDIATAKNLTISELLTEIERIVASGTKLDIKYYIDEYV
                     DEYHQEEIYEYFREADSDSVEEALVELGEDEFTEEEIRLMRIKFMSEVGN"
     gene            7007..>7341
                     /locus_tag="PKM34_03250"
     CDS             7007..>7341
                     /locus_tag="PKM34_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ82632.1"
                     /translation="MKTYEEIRKIAQQTVRAERDAINGLQDSIGDDFVSLVRLILENQ
                     GRVIITGIGKSAIIAEKIVSTLNSTGTPAVFMHAADAIHGDLGIIQKEDLVICLSKSG
                     ETPEIKVLIP"
BASE COUNT         1790 a         1756 c         1754 g         2041 t
ORIGIN      
        1 gtccggtcgc aaggttccag ctggttatgc tttcatattc attttcacct aattttccga
       61 tgaccggacc ggaggaatag taatcgttgt ggtcgaaatc caattgggat acagaacctg
      121 tgactttaac aggagttcct gaatttttta ccctgaaaat attgttcctg gccatcaggc
      181 tgttgtttcc ctcgatcagg atgccgttgc ttttaccctg tgtttcggaa ttcgtgatat
      241 tgacagaatt aaagcaaacc ctcgaagaat ttaccgattg caaccagacc cccatcactg
      301 ttccgcctcc ggtggtatgg atataattat ttgaaatttc agcatacgat gaattcttca
      361 taaagatgcc gctattccct tccgggacat tctggttgct ctttatatgg gtcaacctgt
      421 tcgatgtgat gaaaatgcgg ctgcttgact gctctgtata aattccgtcg ttcgtattgt
      481 taatgtggtt gccgctaatt aaaatgctat ccgattggat aatatggacg gccctgaaac
      541 atcgttctat tctattatca gataccgtga tccggctggc agcctcctct atccggatcg
      601 tggaatagcc tttttcagtt gctcctttga tgtaattacc ggtgatggta gcgtacctgg
      661 tccccgagcc tgaaatgttg accgcgccgg atcgtccgaa tatgcattca aaataattat
      721 ttcggacccc tatgttacgg cttcctccgt cgatgactat tccgtaatcg gcttcatttc
      781 ttgacgtcag ggcgcaattt gccacctcga tctccgaaca gttccggatg accaaagcat
      841 cgttctggct gctgttggaa ccggtcgaca accccaggtg cctgaacgaa atattttcgg
      901 cgccattgag caaaatcatc ggctcgtatt ttaacgcgtt ggggatgatc cggatcacgg
      961 cctgggtgct gtcaccgctt tccgactcga aagtcacggt attgacagca gaagaaccct
     1021 ggatctgccg gatctcaaac tggtcgaaat aggttccgtt cctgacgata atagtgaccg
     1081 ggcaggtaat gccggctatg tttaaaacat ctgcaatctg agaaaatgac tcgaaatcag
     1141 cttcatttcc acctattgta tatgtaccgc atagaggcgg aacaaggtat tccgatacgg
     1201 tgtcgttata ggggttgaga tcgttctgcc cgttcggttg ggtatgccaa accttgatca
     1261 tgaccggttt ggtaaaattg tagctgccga tcgtaatgtt gttcccggtc tggccaaatc
     1321 ctatggaacc gttccaggta tgggcaggtt gtgcgacatc gtctgccgtc cagtttatgg
     1381 tggtggaagt cagggtatta tatccaaaat tacccaggct tatcctgaca tcctggatgc
     1441 caggggtaac gggtgattcg gggctgtcga ccgtgaccac cccaccgtcc aggggaagtg
     1501 aagcataaat ccggatattg tcgatagccc agaagccact gccattgccc gaccagatga
     1561 accggatccg ggcattggtg atgccgcccg tggcatcgga aaggtccacg atagcgctgg
     1621 ttggattgga ttcggtgggc tgcagttctg tgaccccgac ccaggaggaa ccgttgtaag
     1681 catcaatccg tatcgtagcg ccgcttcccg gatcaaatac atgaacaaaa ttcaatagga
     1741 tataattgct tgtactggca tcaaaaagcg gagattcaag cgcaatctcc tcgggtgagc
     1801 catcgttcga aacgctgtcg ccatcaaaaa ccgcgaaagg ctcggttacg ggataattca
     1861 gcagacggtc accgggattg tcgaaatgcc atttgtcgtc cgcactaccg gcaattacca
     1921 ggttggacca tccttgagga gggacggttc cgctggcacc attgaaatct tcgttcagaa
     1981 taaacatctg agccagcgcc ctgaaggaga gtagcagcgt aactatcaga aatacatacc
     2041 gtttgttcat atagcccggt aagtgtaata tagcgtaaaa tacagcagga tcatcctaac
     2101 aaactttagt ataatctcga ttccatgaat aggtaatatc cattagcgaa tgatctctac
     2161 tttcatacga agaaaatgaa aataggttca gtttttttca acctgttccc ttactttccg
     2221 catgatgttc aggggggtat cggtgatcac catattgatc agccacgacg ggatgttacc
     2281 gcccggatcg accgatccct ccaggaccag gtggaccagg ccgttgccgg catcttccat
     2341 tgtccagcgc tgccagtaat tggtgatcct cacccggtcg gaatattcgg ggaccagccc
     2401 gctgatgggc ttggcgtgaa ctattctttt cccggttacc ggatcgttgg taatgatggc
     2461 ttccacgcat aaatcacggt cgctcaaagg ccagggaaca tcgtaaacga gatagtaccg
     2521 gatcagtttc tcttcaacgt attccaggac ctttatctcc gatatgttat cgtcccacca
     2581 gtcaaattta tcgacccgcc cgataatccg gctgatagcg tccatcccgg tgagaaaatc
     2641 ggcttcccct ttaaaggatt taacagaact ggtctcctcg ctgcgggtat agatccggat
     2701 accttccttt tctttgatga aatcccatgg cggttcctga ccgaatcctt tgaataataa
     2761 ggcattcagc aatataataa ggagtatccg gtaataataa ccgctggtca aacagatttt
     2821 ttttacaaat aaacaccatt tattcttctg aaattagcga ataatccgtg catggaatta
     2881 attttgattt ttttcagtta aaactacatg atgaatcaaa tattcaaaga atacaggttt
     2941 acagaaaaag aactggccgc cagagccgac gggattacag aaaagatact gcctgatgaa
     3001 acggatatag tattgatatc caggcttctg ggatgtattg acctaacgac ccttgaagga
     3061 tccgatactc aaaggaaagt gacggattta tgccggaaag cacagttctc tgccataagc
     3121 gagcggtttc cggaagctgc tgcggtatgc gtttatcctt ctctggttaa aacagccgtc
     3181 gatgagttga aaggtaccgc aatccgggtg gcttccgtgg ccggagggtt tccgtcgggc
     3241 cagacaggat tgagaatcaa gctggaagag gtgaaatttg ctgtaaatga aggggctgac
     3301 gaaatcgata cggtcatcag ccggggaaaa ctgatcgggg gagatgagac ggaagtattt
     3361 gacgagctgt gtgcgataag ggagatgtgt ggcccggtgc acatgaaggt tatcctggaa
     3421 acaggcgaac tgccttcggt gggatatgtg cgacgggcat ccgaacttgc tatcgctgca
     3481 ggagccgatt ttatcaaaac ctccacggga aaaatatcgg agggagccac tgccgtccct
     3541 ttcctggtga tgctggatac gatccgggag taccacgacc tgaccggtaa gaccattggc
     3601 atcaaaccgg ccggcggcat cagaactttc gggaatgcaa ctgtttttac cgcattgctg
     3661 atcgaaattc ttggagacac atggttctgc aaggaaagtt tccggatcgg cgccagcagc
     3721 cttgccgacg atctggtcga caaaggattg cgcctggttt gacgggtgca tatggcgcgg
     3781 catgaaattt gaaggtattt agataaacgt attgatatga aggttcaatt atccctgctg
     3841 gctttgctgg ctttgatgtt attcttcccg gcctgcctcc ctgaagatga agacattgat
     3901 acgggcgacc ccgttgaaaa attccttggc acctggagtg tgaacgaatc atgccgaaga
     3961 ggcaattact cccttgagat tacgcgagat cccggcaaca gcgcgcaggt cctggtgtat
     4021 aatttcggaa acccggggcc cggttatgat cctgccgtag gactggttgt ctcaaacaac
     4081 ctttacattc aatcccagac catcggggag gggtggacgg tcgacggaaa aggaacttac
     4141 acttcaaatg gcagcatttc atgggattat tcgctggtca ttccgccgaa cgaactgaat
     4201 tgtacggccg actcacaccg gcagtgatca ttcaaccgtt ttcttctccg attttacctc
     4261 ttctttccgt tcttcttttc tgggcatgac ctggctctcc tttttaccaa gctcgatgat
     4321 tacctgctca tagattttgg agatttcacg cggatcgtat gtgtaaaacc gcatgctctc
     4381 gtcgaattgc tctttcgtca cattatgcct ggtgaggatc tcattcaggt aggccgacga
     4441 cacggttttg agagaatctt tatgcacttt ccgggcattg attgccccgt cggccagatg
     4501 aagatcggtg atgatgatag ccattgagtc ggcgggtatc agtaacgacc tgttgaattc
     4561 cggcccgggt gcggtatggt aacaggaaac caggaaacta atggcgatga tcgcgctgaa
     4621 tatcctgtgc atcagttccc tacctccgac ataaatttga tgcgcatcag cctgatctct
     4681 tcttcggtga attcatcttc acccagctct accagcgcct cctcaaccga atcggaatcg
     4741 gcttccctga aatactcgta gatctcttcc tggtgatatt catcaacgta ttcatcaata
     4801 tagtatttga tatcgagctt agtgcccgaa gccacaatcc gttcgatctc tgtcaataat
     4861 tcgcttatgg tcaggttttt agctgtagca atatcttcca gggccagttt ccggtcaata
     4921 ttatgaataa tgtacacttt caatcccgat ttgttgacaa ccgatttgac taccatatca
     4981 ttcggccgga tgatgtcgtt ttcttccaca tattgctgga tcagcttcag gaagggatcg
     5041 ccgtatttgt gtgctttccc tgtgcccacg ccggtgattt gtaacagctc ttcggatgtt
     5101 atggggtatt ggatggccat atcctccagc gacggatcct ggaagatcac aaacggaggc
     5161 aggttctcct tccgggagat ctctttgcgg agatccttca gcatcgcgaa caggttctta
     5221 tcggccgtgc tggttttgtg ggccccttcc atgatatcct cttcttcctc caggttgtcg
     5281 tagtcccgat catgcgtgag catgatgctg tatggtttct tcaggaattt atgtccttcc
     5341 ttgctgattt tcaggatgcc gtaattttcg atatccttta ccagcaactg gctgataagc
     5401 gcctggcgga ccgcagcgtt ccagaaccgt tcatcctcat cggccccttt tccgaactct
     5461 tccagcacat tgtgcttgta agatttcatc gtggtagagt tcttgccgat gatgatattg
     5521 acgatatgtt ttgccttgaa ttgttgtttg acggccagca ccgtttcgat cacaagtttg
     5581 atgtagtctt taccctcgaa ttttgctttg ggatgaaggc agttgtcgca cgatccgcag
     5641 ttctccttgg ggaattcctc cccgaaataa tgcaggagaa gtttatgcct gcatacggcc
     5701 gattcggcgt aagcaaccgt ttcctgcagc agctccttgg cgatctcctg ctcggctaca
     5761 ggcttgtctt tcatgaattt ctccagcttc aggatatcgt cgtaactgta aaacgctatg
     5821 caattgcctt cgccgtcatc cctgccgcac cgtccagtct cctggtaata actttccagg
     5881 cttttcggca tatcgtaatg gataacatac cggatatcgg gcttgtcgat tcccatcccg
     5941 aaggcaatag tggctacaat cacatcgaca tcctccatca ggaaccggtc ctggtttgat
     6001 ttgcgggtat tcgattccaa acccgcatga tagggtaaag ccttgatacc attgaccacc
     6061 agtgtctcgg ccatctcttc cacctttttc cggctcatgc agtatacgat gcctgatttc
     6121 cccaggttgg ctttgatata tttaatgaca tcctttgcga cgttttccgt cttgggcctg
     6181 atttcataat aaagattagg cctgtcgaat gatgatttga agatcgtcga ctggggtatc
     6241 tcaaggttct tgatgatatc ctgctgaact ttgggtgtag ccgtagcggt cagggcaatg
     6301 acgggcacat cctgcccgat ggtttcgatt ataggcctga tcctgcggta ttcaggcctg
     6361 aaatcgtgac cccattccga tatgcaatgg gcttcatcga tggcataaaa ggatatgttt
     6421 atattgcgca ggaactcgac gttgtcttct ttggtaagtg attcaggggc aacatataat
     6481 aatttcgtgt atccttccac caggtcttgc ctgacctctt tcatctccga cttactaagt
     6541 gaagaattca tgaaatgggc caggttatgg tcggtgccat agttccggag attgtcaacc
     6601 tggtttttca tcagggcgat gagtggcgat acgatgatgg ctgttccttc gcacatcatg
     6661 gcaggcagct ggtaacagag cgatttgccc cccccagtgg gcataattat aaaagtatct
     6721 ttcctcgaaa gaagatgttt gatgatgatt tcctggttcc ctttaaaatt atcaaaacca
     6781 aagacatcct tcagtacctc cttcatcgat aaggcttcct tgactatcat taatgcaacc
     6841 taaaattgag tatctttgtc agtaaatcag agtatctata actatcgata gcagattata
     6901 tttttataaa tatacgattt tttgaaaaaa gccagacaaa catgaatttt ttaacttttt
     6961 ttaaaatatt ttcccgaaaa attaacagac tgaaatactg attgttttga aaacttacga
     7021 agagatcagg aaaattgcgc aacaaactgt ccgcgccgaa agagatgcca tcaatggttt
     7081 gcaggattcc atcggcgacg atttcgtgag cctggtccgg ctgatcctcg aaaaccaggg
     7141 gcgtgtcatc attaccggaa tcggcaaaag cgccatcatt gcggaaaaga tcgtatcgac
     7201 actcaactcc acgggcaccc cggccgtttt tatgcacgct gccgatgcca ttcatggtga
     7261 cctgggcatc atccagaagg aagacctggt gatctgcctg tcgaaaagcg gtgaaacacc
     7321 ggaaatcaaa gtcctgatcc c
//
LOCUS       DAOQXS010000070         7340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10521_length_7340_cov_4.026081, whole genome shotgun sequence.
ACCESSION   DAOQXS010000070 DAOQXS010000000
VERSION     DAOQXS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10521_length_7340_cov_4.026081"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PKM34_03255"
     CDS             complement(<1..1187)
                     /locus_tag="PKM34_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNQ82633.1"
                     /translation="MVVKIIVLAIYALVIIIVGIYGLRKTRSFNDFFLGGGKVGAIMS
                     AFSYGATYFSAVVFIGFAGKIGWGFGYSGIWIGVLNGLIGVLAVWALLGWRVKKMSVE
                     MGVHTLSEYLEKRYGSPALKLIASIAIFIFLIPYSAAVFIGLSYLFASSFEGLQYWHA
                     VLFMGVFTAIYIVLGGYKSMAMMDTVFGIIMTTGVLVLFGYTLNAGGGLAAITEKLQS
                     IDPGLTATVGPPGWWPLLSLILLTSVAPFAMPQLMQKFYAIKDRKAIRTGMIASTIFA
                     LLIGGIAYFLGSTTRIFLAPETSPAAFTAEGKPVFDALMPELLARVIPESLSIIILLI
                     ILAASMSTLASLVLISSSSFCKDFYAGFINRNVTDKQLTNLMRGANLFFVLLSVILAL
                     IKPS"
     gene            complement(1247..4864)
                     /locus_tag="PKM34_03260"
     CDS             complement(1247..4864)
                     /locus_tag="PKM34_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ82634.1"
                     /translation="MIFTPKLLRNTTILIFILLIFKITYSQSRQDNPVVWTGITSDTP
                     MESRIKLVSSDITTNRIEIITEGFLKEIIRDNEGGKEVVVSAENTTPLLIEGAPDLGK
                     LSIPLAIPDLDRMTVTVVEADFIDFQNILVIPSKGNLTRDINPDDVPYTYGREYRTDA
                     FFPGVAAELRTPHIIRDVRAQVVVAYPFRYNPVSKVLRVYTRLVLDIASAGEDGENPY
                     VLRREDGKTVRSFEEIYQRKLLNYKEYRTASRYTPLEEDGNMLIICYGSFMADMQPFV
                     DWKNMSGIPTEMVNVSSVGTTAAAIKSYVANYYNTNGLTYLLLVGDAAQVPVSSTTAG
                     PSDNDYGYIVGSDHYPDIFVGRFSAETNAHVQTQVQRTLNYEKQPDLSPGFFERAMGI
                     ASAEGPGDDGEYDWQHQRNIRTDYMGFTYTYGAELYDGSQGGQDAAGNPTATMVASEI
                     NTGTGIISYTGHGSNTSWSTTGFSNTNITSLTNTNKLPFILSVACVNGNFAGITCFAE
                     AWLRATYNNQPSGAVATIMSTINQSWNPPMQGQDEMVDILVENFPSNIKRTFGGICMN
                     GCMGMNDVYGSAGADMTDTWTIFGDPSLMVRTAAPVTIAVDHPADLPVGSDEITVLCD
                     QEGARVAVSRDFELLGSGYVSGGTALVEFGQINTEDTLIVTVTAFNCMPYIDTVFVTN
                     AEMVYVSSQAFHGNLTEVAPGQQDMEALGVKILMSGSVNPFNLLSLTFNMNGTTDLED
                     VESLAVYYTGNNGSFGTATLFGIGVVSAGSITVTGNQPLNGGFNYFWLAYDLAEDALM
                     GHFLDAECTGMALGDNPALTRVPAITDPDGQREINRMSLVSLTCSQPVTSPVPPGTVN
                     CEIIRLEITTDGSPLPLTVTGIKVATQGCTDLDDISAIKVYYTDLPAFSTTNLFGSVA
                     PASFVTVTGNRALTEGSDYFWIVFDISSAAGPGNELDAECTEVTFSGATGTQVPLVTD
                     PPGYRSIMLNYCTPTYTYGSGDGDYISLVSIGSLSKATGASSSPYYNYYQDETTHLVP
                     GSTYQLTLSPGTYGSGNNMAAWIDYNRNGTFESSEKLGELTNMSPTPATGTLTFTVPV
                     SAIHGILRLRVREVWNTTGLDPCAGYDYGETEDYDVHIMPPGCWLGYTNEWTNVSNWS
                     DGVVPGPGTEVIIPEILMGSYYPAVFGNSNLEIEQLNMQEGSNLSIPVGVTITVGGNR
                     K"
     gene            complement(4928..6256)
                     /locus_tag="PKM34_03265"
     CDS             complement(4928..6256)
                     /locus_tag="PKM34_03265"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ82635.1"
                     /translation="MIKLKDIRPIVQGDKKVIRGWVMYDWANSVFQLTITTAIFPLYY
                     DAITRNGGSYTVSFFGAEVINTVLYSWTIGAAFLMVALLSPLLSSIADYTGRRKAFMQ
                     VYTWVGALGCSMLFFFTGDNIELGIIAFFFGTWGYAGSIVFYNSFLPVIAKPEDHDKI
                     SARGYSMGYLGGVVLLLMQLVVIQKPSWFNIPAGSDLQFRLAFLTVGIWWIGFSQITF
                     SRLPKYVFKKRQGKENVILNGYKELRTVYYQVRKSKKLTLYLVSYFFFMMGLLSVMYM
                     AASFGKKEVNLGDEIMIPTLLLIQLIGMGGAWFFARLSGKIGNLKALIIALITWILIC
                     SGAYFITDAIGFITAAFFIGIVMGGTQALARSTYSKMLPETTDHTSFFSFFDVMEKMA
                     SAGGTFSFGLIEAITGNMRASVVAIAIFFIIGLFFIIVMPKRGTQNSAEI"
     gene            complement(6350..>7340)
                     /locus_tag="PKM34_03270"
     CDS             complement(6350..>7340)
                     /locus_tag="PKM34_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ82636.1"
                     /translation="IGRAERKIISREFLYDIAVEGPRVILRLNGIQVYDYNTGANLWS
                     AAFDFTPNGVRSRPPNARQWGVYGAVADPVFDKENNVIYVLDMSNRNNQFVKKYDANT
                     GKLIWTSPEIKGAKAIPGMYVVDDKVILQIGGIVEFQSYEVIQTQYSTITIWRVFYDN
                     VKPNGLQAFNTKDGSLAWESEKFKKGITNAYVLENKILCSSGKELYKIDYKTGNEEYS
                     VPVTDGGVGLAVKILPYKDKMIVVGEKGISAYNPVDGKFIYSGKYKASSLEDVFDNIL
                     IMKTEKADIASFNLDTGVFKEFKAKTGASTTLTTDGKFVYIYEDKVFTKVKTL"
BASE COUNT         1869 a         1869 c         1827 g         1775 t
ORIGIN      
        1 gacggtttga tcagggccag gatgaccgac agaagtacaa agaagagatt tgctccgcgc
       61 atcaggttgg taagctgttt atcagtcaca ttccggttga tgaacccggc gtaaaaatct
      121 ttgcagaatg aggaactgga gatcaggacc agcgaagcca gggtcgacat ggaagcggcc
      181 aggatgatga gcaggatgat gatcgataac gattccggta ttacacgggc cagtaattcc
      241 ggcatcagtg catcgaaaac cggtttccct tcggcagtaa aagcggccgg cgatgtttcg
      301 ggggccagga aaatcctcgt ggtagaaccg aggaaatagg caataccccc gatcagcagg
      361 gcgaaaatgg tcgaggcgat catgccggtc cggatcgctt tccggtcttt gatcgcatag
      421 aacttctgca tgagctgggg catggcaaaa ggagccacgc ttgtcagcag gatgagcgaa
      481 agcagcggcc accagcctgg cgggcctacc gtagctgtca gcccgggatc gatcgattgc
      541 agcttttcgg tgatcgcagc caggcctcct cccgcattta acgtatatcc gaacaaaacc
      601 agcactcccg tcgtcatgat gatcccgaat acagtatcca tcatcgccat cgacttatat
      661 cctcccagaa caatgtaaat agctgtaaaa acgcccatga acagcacggc atgccagtat
      721 tgcaagcctt cgaacgagga ggcgaaaaga taggagaggc cgatgaaaac ggccgcagag
      781 taggggatca ggaaaatgaa gatggctatt gaggcaataa gtttcaacgc cggacttcca
      841 tatctttttt caagatattc actgagggta tgtaccccca tttcaaccga cattttttta
      901 actctccagc ccagcagcgc ccaaacggcc agaactccta tcaatccgtt caaaacacct
      961 atccagattc ccgaatagcc gaatccccac ccgatttttc ccgcaaaacc aataaaaaca
     1021 acagctgaaa aataggtagc accgtatgaa aaggcgctca tgatcgcccc cacctttcct
     1081 cccccgagga agaaatcgtt gaaggaccgc gtttttctca atccgtaaat ccctacaatt
     1141 ataataacga gggcataaat agccagaaca ataattttta ccaccataca cgaagatatt
     1201 tcaaataccg gggcaagtta gcaaaaaccc ggcagatgag gcaggattat ttcctgtttc
     1261 cgccaacggt gatcgtaacg ccgacgggga tgctcaggtt cgagccctcc tgcatgttca
     1321 actgttcgat ctccaggttg gaattgccaa aaacagccgg ataatagctg cccatgagga
     1381 tttcgggaat gatgacctct gttccgggcc cggggaccac gccatcgctc cagttcgata
     1441 cgttagtcca ttcattggta taacccagcc agcagcccgg gggcatgatg tgcacatcat
     1501 aatcctcggt ttcgccataa tcatatccgg cgcaaggatc cagtccggtg gtgttccata
     1561 cctctctgac cctcagcctc agaatcccat ggattgcaga tacaggaaca gtaaaagtca
     1621 aagttccagt cgcaggcgtt ggcgacatat tcgtcagctc gcccagtttc tcggaagatt
     1681 caaaagttcc gttccggtta taatcgatcc aggcggccat gttgttcccc gaaccgtagg
     1741 ttcccgggct aagggttagc tggtaagtac ttccggggac taaatgagtc gtttcgtcct
     1801 ggtaataatt ataataaggg gatgaagagg ccccggtggc tttcgaaagc gatccgattg
     1861 aaaccaggct gatataatcg ccgtcccccg atccatacgt ataggtgggg gtgcaataat
     1921 tcagcatgat gctccggtag ccgggaggat cggtgacaag cggcacctgt gtgcctgtcg
     1981 ccccgctgaa agtaacttct gtgcactctg cgtcgagttc attgcccggc ccggcagcag
     2041 aagaaatatc aaatacgatc cagaaataat cggaaccttc ggtcagtgcc cggtttccgg
     2101 ttacagtaac gaagctggcc ggtgctacac tgccgaaaag gtttgttgtt gaaaacgccg
     2161 gcagatcggt gtaataaacc tttatcgcgg aaatatcatc caaatcggta catccctggg
     2221 tggcgacctt gatgcccgtc acggtcaggg gtaaaggcga tccatcggtc gtgatctcaa
     2281 gccggatgat ttcgcaattg actgtaccgg gcggaacagg gctggttacg ggctgtgaac
     2341 aggtgagcga gaccagcgac atccggttta tttccctctg accgtcagga tcggtgattg
     2401 ctgggacccg ggtcagtgca ggattatcgc ccagggccat accggtgcac tccgcgtcga
     2461 gaaaatgacc cataagcgca tcttcagcca ggtcgtacgc cagccagaaa taattgaaac
     2521 cgccgttgag aggctgattc ccggtcaccg tgatcgatcc tgctgaaacc acgcctatac
     2581 cgaaaagtgt tgccgtcccg aatgatccgt tattaccggt ataataaact gccagacttt
     2641 caacatcttc caggtcggtc gtcccattca tgttgaatgt cagactaagg aggttgaacg
     2701 gatttacgga gcccgacatc aggattttta cgccgagcgc ctccatatcc tgctgaccgg
     2761 gggccacttc cgtgaggtta ccatggaaag cctggctgga aacataaacc atctcggcat
     2821 tggttacgaa cactgtgtcg atatacggca tacaattgaa tgccgtaacg gtcacaatca
     2881 gcgtatcttc cgtattgatt tgtccaaatt ccaccaatgc agtgccccct gaaacataac
     2941 cggaccccag gagctcaaag tcgcgggaaa cggccaccct ggctccttcc tggtcgcata
     3001 aaaccgtaat ctcgtcagaa ccaacgggca aatctgccgg atgatcaacc gctattgtta
     3061 ccggggctgc tgtgcgcacc ataagcgacg gatcgccgaa aatggtccag gtgtcggtca
     3121 tatctgcacc tgccgaaccg taaacgtcgt tcatgcccat gcaaccgttc atgcagatac
     3181 ccccgaaagt ccgcttgata ttcgagggga aattctcaac caggatatcc accatttcat
     3241 cctgcccctg cataggcggg ttccagctct ggttgatggt cgacatgatg gtggccacag
     3301 cgccggaggg ctggttgttg taggttgccc tgagccaggc ttcggcaaaa caggttattc
     3361 ctgcaaagtt tccattcacg cacgccacag aaaggataaa gggcagtttg ttggtatttg
     3421 tcaggctggt gatattggta ttactgaaac ctgtcgtgct ccaggaagtg ttgctgccat
     3481 gcccggtgta ggaaatgatc ccggtgcccg tattgatctc actggcaacc attgttgctg
     3541 tcggattccc tgcggcatcc tgtcccccct ggctgccatc ataaagttcg gcaccatagg
     3601 tataggtgaa tcccatgtaa tccgtccgga tattccgttg gtgctgccag tcgtattccc
     3661 cgtcatcccc ggggccctcc gccgacgcaa tgcccatggc ccgctcaaaa aagcccggcg
     3721 aaaggtccgg ctgtttctca taattcaatg tgcgctgaac ctgagtctga acgtgggcgt
     3781 tggtttcagc agaaaatcgt ccgacaaaaa tgtcgggata atgatcactg ccgacgatgt
     3841 agccgtagtc gttgtctgat ggtcctgccg tggtggatga aaccgggacc tgggccgcgt
     3901 cgccgaccag cagcaggtat gtcagcccat tggtgttgta atagttggcc acatatgatt
     3961 ttattgccgc tgccgttgtc ccgacactac tgacattgac catctcggtc gggatcccgg
     4021 acatattttt ccagtcgaca aaaggctgca tatcagccat gaatgatccg tagcaaatga
     4081 tcagcatgtt cccgtcttcc tcaagcgggg tatatctcga agccgtccgg tattccttgt
     4141 aattcagcaa cttccgctgg taaatttcct caaagcttct taccgtcttg ccgtcctccc
     4201 gtcttaaaac ataaggattc tccccgtctt cacctgccga ggcaatatcc agaaccagtc
     4261 gtgtatagac ccgcagcact ttcgacaccg gattatagcg gaaaggatag gccacgacca
     4321 cctgggccct gacatccctg attatatgcg gagtgcgcaa ttcggcggca acacccggga
     4381 aaaaggcgtc ggtgcggtac tcccttccgt atgtataagg gacgtcatcg ggattgatat
     4441 cgcgggtgag atttcctttt gaagggatta ccaggatatt ttgaaagtca atgaaatccg
     4501 cctccacgac ggtgacagtc atccggtcga ggtcggggat ggccaggggg atgctcaatt
     4561 tccccaggtc aggggcgcct tcgatgagaa gcggagtggt gttttcagca gagacgacaa
     4621 cttccttgcc accttcatta tccctgatta tttctttcag gaatccttcg gtgattattt
     4681 cgatacggtt cgtcgtaata tccgaagaca ccagtttgat ccgggattcc attggtgtgt
     4741 cggaggtaat gccggtccag acaactggat tatcctggcg actttgtgaa taagttatct
     4801 tgaaaattag aaggatgaaa ataaggatgg tggtgtttct gagcaattta ggcgtaaaga
     4861 tcattttaat ggatgttaaa atcatccgct aaagataagc acaaatcccg caattaaaac
     4921 tcaaaactca aatctcagca ctgttttgcg ttcctctttt aggcataaca atgatgaaga
     4981 aaaggccgat gataaagaaa atagcgatgg ctaccacact ggccctcata ttaccggtta
     5041 tggcctcaat aagtccgaaa ctgaaagttc cgcctgccga agccatcttt tccattacat
     5101 cgaagaagct gaaaaaagag gtgtggtcag tcgtttccgg gagcatcttg gaatatgttg
     5161 accgggccag cgcttgtgtt ccacccataa cgatcccgat aaagaatgct gctgttatga
     5221 atccgatagc atctgtaata aagtaagcac cggagcagat caggatccag gttatcaggg
     5281 cgataatgag tgctttcagg ttgccgatct tcccggatag ccgggcaaag aaccaggccc
     5341 cgcccatacc gatcagctgg atcagcagca gtgtggggat catgatctcg tcgccgaggt
     5401 tcacctcttt tttcccgaaa gaggcggcca tatacatcac cgacagcaag cccatcatga
     5461 aaaagaaata actgaccagg taaagggtca gtttctttga ttttcgtacc tggtaataaa
     5521 cagtccgaag ttctttgtac ccattcagaa tgacattttc tttcccctgt cgttttttga
     5581 agacatactt gggaagcctt gaaaaagtaa tttgcgaaaa gcctatccac cagataccta
     5641 cagtcaggaa agccaggcgg aactgcaggt cgctgccggc cgggatattg aaccaggaag
     5701 gtttctgaat gacgacgagt tgcatcagta aaagcacgac gcctcccagg tagcccatgg
     5761 aatagccccg ggcgctgatc ttgtcatgat cttccggctt tgctataacc gggagaaaag
     5821 agttgtagaa aacgatgctg ccggcatatc cccatgtgcc aaagaaaaag gcgatgatcc
     5881 cgagttcaat gttgtcgccg gtaaagaaaa acagcatgga acagcccagc gccccgaccc
     5941 aggtgtacac ctgcatgaaa gcttttcgac gtccggtata atccgctatc gacgagagta
     6001 atggcgacag cagcgccacc atcaggaatg ccgcgccgat cgtccatgaa taaaggaccg
     6061 tattgatgac ttcagctccg aaaaagctga ccgtatagct cccgccgttc ctggtgatgg
     6121 catcgtagta caaggggaaa atggcggtag tgatggtcag ctggaatacc gaattggccc
     6181 agtcgtacat cacccagccg cggatgactt tcttatcacc ctggacgatt ggcctgatat
     6241 cttttagctt gatcatggac ggggattttg aggtgaaggt aaaaaaaatc gcaggtctga
     6301 caattcagcc tgcgattttt ttaatatgat aatcaataag atatgattct tacaatgttt
     6361 ttactttggt aaagacctta tcttcataaa tataaacaaa cttaccatcg gtcgtcaggg
     6421 tggtcgatgc gccggtcttc gccttaaact ccttgaaaac gcccgtatcc aggttgaacg
     6481 aagcgatatc cgctttttcg gtcttcatga tcaggatatt gtcgaataca tcctccaggc
     6541 tggaagcttt atattttccg ctgtagatga atttaccgtc aacaggatta taggcggaga
     6601 ttcctttttc acctacaacg atcatcttat ccttgtaagg caggatcttc acggcaagtc
     6661 cgacgccgcc atcggtaacc ggaacggaat attcttcatt tccggtctta taatcgatct
     6721 tgtaaagttc tttcccggac gagcaaagga tcttgttctc taacacatag gcattggtga
     6781 tccctttctt gaatttttcc gattcccagg ccagcgaacc gtcttttgtg ttgaatgctt
     6841 gtaatccgtt gggtttcacg ttatcataaa acactctcca aatggtaatg gtagaatact
     6901 gggtttgtat gacctcataa gactggaatt cgacgatacc cccgatttgc aggataacct
     6961 tatcatccac aacatacata ccgggaattg cctttgcccc tttgatttcg ggtgatgtcc
     7021 agataagttt gccggtattg gcgtcgtatt tttttacaaa ttggttgttg cggttcgaca
     7081 tatcgagcac atagatcacg ttgttctcct tgtcgaatac cggatcggca acagctccgt
     7141 aaacgcccca ctgtcttgca tttggcggtc tcgacctgac gccgttgggg gtaaagtcga
     7201 atgcggccga ccacaggttg gcgccggtgt tgtaatcgta aacctggata ccgttcagcc
     7261 tgaggatgac ccgggggccc tccacggcaa tatcatacag gaattcacgg gagatgattt
     7321 tccgctcggc gcgtccgatg
//
LOCUS       DAOQXS010000071         7239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10705_length_7239_cov_3.456988, whole genome shotgun sequence.
ACCESSION   DAOQXS010000071 DAOQXS010000000
VERSION     DAOQXS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10705_length_7239_cov_3.456988"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKM34_03275"
     CDS             complement(<1..566)
                     /locus_tag="PKM34_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82637.1"
                     /translation="MFKNFLISAFRNLLRNKFYAFLNILGLSVGLAAFIFILIYVLDE
                     INYDKHNEKHARIYRIESDFNISNRHDRFAIVPVPMGPAFKLEFPEVEAVTRLNPVGN
                     ALFRYGEKEYYEDDFYFADSNIAEVFTLNYLSGVPEKALNEPFTMVLTKKIAKKYFGD
                     ANPMGEIITTGSGRSYKVTGVIEDLPANS"
     gene            complement(615..1292)
                     /locus_tag="PKM34_03280"
     CDS             complement(615..1292)
                     /locus_tag="PKM34_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82638.1"
                     /translation="MIKTVNLTKVFRTDEVETTALNNVSFNINPGEFVAIMGPSGCGK
                     STLLNILGLLDNPSGGEYYFNDAEVSRFNERQRARMRKENIGFVFQNFNLIDELNVFE
                     NVELPLIYLGMTATERKRRVNEALEQMQITHRRKHFPLQLSGGQQQRVAVARAVVANP
                     KLILADEPTGNLDSANGEEVMNLLERLNQQGTTLIIVTHSQRDAEYAHRIIRLFDGQI
                     VTENIRA"
     gene            complement(1351..3981)
                     /locus_tag="PKM34_03285"
     CDS             complement(1351..3981)
                     /locus_tag="PKM34_03285"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNQ82639.1"
                     /translation="MNKFCLTALMIFLLVNIPGINNTYSQERREYRAVRISGSAPAID
                     GRADEPAWEEAEWQGGFIQQRPFDGKPASQLTEFRIIYDDEAIYALIRAFDTEPEKVE
                     KRLTRRDETEGDVVALQFDSYFDRRTAYSFMVNAAGVKKDYIISNDGDDDKTWDPVWF
                     VKTSTDSLGWLAEMKIPLTQLRFAKAEDYLWGMQVGRILYRLDELSLWQPIPADAPGW
                     VSHYGNMTGMTNIVPKREVELLPYIMGNMENYPEETGNPYAEGRDFGFNAGVDGKIAI
                     TNDLTMNFTINPDFGQVEADPSVVNLTAFETFYPEQRPFFIEGNNIYSFKLTGGNGSM
                     GRDNLFYSRRIGRTPHYCPEITDDEYLKMPRNSTILGAFKLSGKTRDGWSVGIMESVT
                     QQESALKYSGGKETRITAEPLTSYFNTRVQKDFSQGETILGGMVTATNRFINDSALDF
                     INYSAYTGGLDFTKYWKDRTYYVAVNGAFSHLTGKSEAITEIQSSPVHYFQRSDNDHR
                     DLDSGRTSLTGTGGAFEIGKQGNGHWQYMARFVWRSPGLELNDMGYLRQGDIIQEVLW
                     ANYRIWEPFGIFRSMNISGDHYTGWDYGGNYVYQGIECNSNFEFKNYWSFSPGFEIGF
                     NSVDRYNLRGGPAIKNPGGASLWGFLETDPRKKLTTGAGIVYHKANYDNARILRLELA
                     LTYKPVNSLQISLSPFWETGHDRLQYVETLESNSENRYIMARIDNQILSANLRINLSI
                     TPDLTIQYWGQPFVFAGNYSEFKRDASTRADYYYDRFHIYPEDEIFFNEADNEYTVTE
                     SGSTLSFSNPDFKVFEFRSNLVVRWEYIPGSAAYLVWSQGRIGDDISGESEFGPDIDR
                     LFSIEPHNVFLLKLTYRISI"
     gene            complement(3974..6388)
                     /locus_tag="PKM34_03290"
     CDS             complement(3974..6388)
                     /locus_tag="PKM34_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82640.1"
                     /translation="MFNNFIRILLRGFVKRRVYTVINLLGLAIGLTSFILIMLYVRDE
                     LSFDRHYRNSDRIARVCMIYDFGGVGENSASMPFPVAYTLKSEYPDLVENVVRIFNFQ
                     SDRSLVEYEGKKFSESRFYFADSSFFEIFSQEFIKGDPATALLEPHSVVITEKMAEKY
                     FPGEDPYGKILKYEVNLPLKVTGVVKEPVSQTHFRFDFIASLSTMKKVFNGRLPQTWV
                     WNPCWTYILMPEGAQPARLEEKFPAFIQKYYYDAQRESIVMYLQKLTDIHLHSRIDYE
                     IRPNSNYMYVVVLSAIAVFLLLIAAINFMNLATATAGSRAREIGIRKVTGADRMRLIG
                     QFLGESLAMTLLAFILALILIQAILPSFNDLTGKDIQTGYLADPINLAWLAGLWLLLG
                     LLSGAYPALFLSSFKPIAILKPGLFINSRSGLPRKILVVFQFTISIGLILSTMMIFRQ
                     VNFLRNAEMGFDPENIIMVRINQTKVSKDFETFRNELIRNPNIESVTALDDIIGESHN
                     THEFWFEGMKEKEWRFFPALVVRYDFLQTFGIELVAGRDYNRENKTDPEQALLVNEAL
                     VKHMGWSSNDEALGKRFHSLNGDEKIVGVFRDFQPTSFREPAGPFILNMKEKAWEINF
                     FMKYAAIRTTGKNNAEAIRYIESKWSEFEKEKPFDYFMLKDELKRLYNDERNLGNLAV
                     AFTFLILFVAAMGLFGLASFMAEKRTKEIGIRKVMGASVFDILMLLQREFTWLIVIAM
                     IIAWPVSYFLVDSLFLQQFALRVPFNIWVFLLSGFFALAISLLIISYRALKASLINPV
                     ETLKYE"
     gene            complement(6381..6644)
                     /locus_tag="PKM34_03295"
     CDS             complement(6381..6644)
                     /locus_tag="PKM34_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82641.1"
                     /translation="MREIVIIVPDFEPEQNVEIEVTINGRKKVIHYRVELVNFEAEGV
                     PPKDKVTVLKHKIAEHEKDWELMEIGAPQGENVPLMFRRKVNV"
     gene            complement(6781..>7239)
                     /locus_tag="PKM34_03300"
     CDS             complement(6781..>7239)
                     /locus_tag="PKM34_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HNQ82642.1"
                     /translation="MDGYKMRANIDERYVSRVVPGQEAELDFGGKTYQLYVYKIYTGV
                     TGGAFQVDMMFRDEVPANIKRGQTIQMRLQFSTPTEALILKRGGFFQQTGGNWVYVVD
                     PSGKYAVKRNIKLGRQNTRFYEVLEGLEAGEEVIVSSYDSFGEKDKLVLR"
BASE COUNT         1665 a         1909 c         1808 g         1857 t
ORIGIN      
        1 gaattggccg gcagatcttc gataacgcct gtcaccttgt atgaccggcc tgatccggtg
       61 gtgatgatct cgcccatggg attcgcatcg ccgaaatatt tcttcgcgat cttttttgtc
      121 agcaccatgg tgaaaggctc gttcagggcc ttctccggca cgcccgacag gtaattaagc
      181 gtaaacactt cggcgatatt ggaatcggcg aaatagaaat catcctcata gtattccttt
      241 tctccgtatc ggaacagggc attaccgacc gggttcaggc gggtaacggc ctccacctcc
      301 gggaactcca gcttgaatgc aggccccatg ggaacgggga cgatagcgaa ccggtcgtga
      361 cggttggata tattaaaatc cgattcgatg cggtagatgc gggcatgttt ttcgttgtgt
      421 ttgtcgtaat tgatctcatc caggacatag atcaggatga agatgaaagc ggccaggcca
      481 accgatagcc cgagtatgtt gagaaaagca tagaacttgt ttctcagcaa attacggaat
      541 gcggatatga ggaaattttt gaacatggta ttctctttca actttgcact ttgaactttg
      601 cactttgaac tacttcaggc tctgatgttt tccgtcacga tctgcccgtc gaacaggcgg
      661 atgatgcggt gtgcgtattc ggcgtcgcgc tgggaatggg tgacgatgat gagtgttgtc
      721 ccttgctggt tgagcctttc cagcaggttc atcacctctt cgccattggc tgagtcgagg
      781 ttaccggtag gttcatcggc caggatcagc ttcgggttag ctacgacagc ccgggctacg
      841 gcgacacgct gttgctgacc tcccgaaagt tgcagtggaa aatgcttccg tcggtgggtg
      901 atctgcatct gctccagagc ttcgttgacc cgtctctttc tttccgtggc cgtcatgccg
      961 aggtagatca gcggcaactc cacattctcg aatacattca gctcatcgat caggttaaaa
     1021 ttctggaaaa cgaaaccgat gttttccttg cgcatcctgg ctcgctgcct ctcattgaac
     1081 cgggatacct ccgcatcatt gaagtaatac tccccgccgg aaggattgtc aagcaggcca
     1141 aggatgttca gcaaagtcga ttttccgcat ccggagggcc ccataatggc gacaaattcc
     1201 ccgggattga tattgaaact gacattgttc aaggcggtgg tctccacctc atcggtgcgg
     1261 aatacttttg ttaaattgac ggtcttgatc attttgtttg atttttatta tttgactgtt
     1321 ttgagttgca tttagttaca atactatctc tcatatgctg atcctgtatg ttaatttcaa
     1381 aagaaaaaca ttgtgaggtt cgatgctgaa caaccggtcg atatcgggtc cgaattcgga
     1441 ctcgccgctg atatcatccc cgatccgccc ctgcgaccag accaggtaag cagccgaacc
     1501 ggggatatat tcccatctta caacgagatt ggaacggaat tcgaaaactt tgaaatcggg
     1561 attggaaaaa cttagcgtcg atccgctttc agtcaccgta tattcattat cagcttcatt
     1621 gaagaagatc tcatcttccg gatagatatg gaaccggtca taataatagt ctgcccgggt
     1681 ggaggcatcc cgtttgaatt ccgaatagtt gccggcaaat acgaacggct gcccccagta
     1741 ctgaatggtc aggtcggggg tgatactgag attgatccgg agattggccg acaggatctg
     1801 gttatcgatc cgggccatga tataacggtt ttcggagtta gattcaaggg tttcgacgta
     1861 ctggagccgg tcgtggccgg tttcccagaa cggcgagaga gagatctgaa gtgaattgac
     1921 cggtttgtag gttagcgcca gttccaggcg cagtatccgg gcattgtcgt agtttgcctt
     1981 gtgataaact atcccggcgc ctgtcgtcag tttcttacgc ggatcggttt ccaggaatcc
     2041 ccacagggaa gcaccccccg gattctttat ggccggcccg ccacgcaggt tgtaccggtc
     2101 gaccgagttg aaaccaatct caaatcccgg cgaaaacgac cagtaatttt taaattcgaa
     2161 gttgctgttg cattcaatac cctggtaaac ataattgccg ccataatccc agcctgtgta
     2221 atgatcgccg ctgatattca tagacctgaa tattccgaac ggttcccaga tcctgtagtt
     2281 ggcccagagc acctcctgga taatgtcgcc ctgcctgagg taccccatat cgttcaattc
     2341 cagcccgggc gaacgccaga caaacctggc catgtactgc cagtgcccgt tgccctgctt
     2401 cccgatctcg aatgcgcccc cggtgccggt taaggaagtc ctgcccgaat ccaggtcccg
     2461 gtgatcattg tctgacctct gaaagtaatg aaccggcgag gactggattt cggtgatggc
     2521 ttcggatttg cccgtaagat ggctgaaagc gccgttcacc gcaacgtaat aggtcctgtc
     2581 tttccagtac tttgtgaaat ccaggcctcc cgtgtaggcc gaataattaa taaaatcaag
     2641 cgctgaatca ttgatgaaac ggttggtagc tgtgaccatc ccgccaagga tcgtctcccc
     2701 ctggctgaaa tccttctgaa cacgggtatt gaaatagctg gtcagcggtt cggccgtgat
     2761 ccgggtttct ttaccaccac tgtattttaa tgcagattcc tgctgggtga cactttccat
     2821 tattccgacc gaccacccgt cgcgcgtttt gcccgacaat ttgaaagcgc ccaggatcgt
     2881 agaattccgc ggcattttca ggtattcgtc atcggtaatc tcaggacaat aatggggcgt
     2941 cctgccgatc ctcctggaat agaacaggtt gtctctcccc atcgaaccat tgccgccggt
     3001 cagtttgaag ctgtagatgt tgtttccttc tatgaaaaag ggcctttgct cgggatagaa
     3061 tgtctcaaaa gcagtcaggt tcaccaccga cggatcggct tccacctgtc cgaaatcggg
     3121 attgatcgtg aagttcatcg tcaggtcgtt cgtgatggcg atcttcccgt cgacaccggc
     3181 attgaacccg aaatccctgc cttcggcata cggatttccg gtctcttccg gataattttc
     3241 catgttgccc atgatgtagg gtaacagctc cacttcccgc ttcgggacga tgttggtcat
     3301 gccggtcatg ttgccgtaat ggctcaccca gccgggcgca tcggccggga tgggttgcca
     3361 taacgacaat tcgtcgagac ggtaaagtat gcgtccaacc tgcattcccc agagataatc
     3421 ctcggctttg gcaaaccgga gctgcgttaa ggggatcttc atttcggcca gccatcccag
     3481 tgaatccgta gaagttttta caaaccacac cgggtcccag gtcttgtcat catccccatc
     3541 attggaaata atataatcct tcttgacgcc tgcggcattg accatgaaag aatatgccgt
     3601 gcgccggtcg aagtagctgt cgaactggag ggccactaca tccccttcgg tttcgtcgcg
     3661 ccgggtgagc cgtttttcaa ctttttcggg ctcggtgtca aaggccctga tcaatgcata
     3721 tatcgcctca tcgtcataaa tgatccggaa ttcggtaagt tgcgaggccg gttttccgtc
     3781 aaatggcctt tgctggataa atccaccctg ccattcggct tcttcccagg ccggctcatc
     3841 ggcccgcccg tcgattgccg gtgcgctgcc cgaaatcctg actgcgcggt attcccgcct
     3901 ttcctgagag taagtattat ttatccctgg aatattcacc agaagaaaga tcatcaaggc
     3961 agtcaggcag aacttattca tatttcagtg tttcgaccgg gtttatcaat gaggctttca
     4021 atgcgcggta cgagatgatc agcagggaga tggccagggc gaaaaatccg ctgagcaaga
     4081 atacccagat gttgaaaggc actcttaagg cgaactgctg cagaaagagc gaatcgacca
     4141 ggaagtagct caccggccac gcgatgatca tggcgatgac tatgagccat gtaaattcgc
     4201 gctgcaacag catcaggata tcgaaaaccg atgcgcccat cacttttcgt atcccgatct
     4261 ctttggtcct cttctcggcc atgaacgaag cgaggccgaa aagccccatc gccgccacga
     4321 aaaggatcag gaaagtgaag gccacagcca ggttgccgag gttgcgctca tcgttgtaca
     4381 atctcttcag ctcatccttc agcataaaat aatcaaaagg cttctctttt tcaaattcgc
     4441 tccatttcga ttcaatatag cggatcgctt cggcgttgtt cttacctgtt gtccggatgg
     4501 cggcgtactt catgaagaaa ttgatttccc atgctttttc tttcatattc aggatgaacg
     4561 gaccggccgg ctcacggaaa gaggtcggct ggaaatcgcg gaacacgccg acgatcttct
     4621 catccccgtt aagggaatga aaccttttac ccagcgcctc atcattggaa gaccatccca
     4681 tgtgctttac cagcgcttca ttgaccagca gcgcctgctc cggatcggtc ttgttttccc
     4741 ggttgtaatc ccttcccgct accagttcga tgccgaaggt ctgcaggaaa tcataccgga
     4801 ccaccagggc ggggaagaag cgccattcct tctctttcat cccttcaaac cagaattcat
     4861 gcgtattgtg cgattccccg atgatatcgt cgagggccgt tacgctttcg atattcgggt
     4921 ttcggatcag ttcgttacgg aaggtctcaa aatccttcga caccttggtc tggttgatcc
     4981 tgaccatgat gatgttttcc ggatcgaagc ccatctcggc attacgcagg aagtttacct
     5041 gccggaatat catcatcgtg ctcagtatca atccgatgga aatggtgaac tggaagacca
     5101 cgaggatttt ccggggcaga ccgctccggg agttgatgaa caagcccggt ttcagaatgg
     5161 cgatgggctt gaaagatgac aggaacaggg cggggtatgc gcctgatagc agtcccagca
     5221 ggagccagag accggcaagc caggccaggt ttatggggtc ggccaggtaa ccggtctgga
     5281 tatcttttcc cgtcaggtcg ttgaaggagg gaaggatggc ctggatcagg atcagggcga
     5341 gaataaacgc gagcagggtc atggccagtg attcgccgag gaactgcccg atcagccgca
     5401 tccggtcggc gccggtcact ttgcggatgc cgatctccct ggccctcgag ccggctgtgg
     5461 ccgttgccag gttcatgaag ttgatcgcgg cgatcagcag cagaaatacc gcaatggccg
     5521 acaagacgac cacatacatg taattgctgt tgggcctgat ctcataatcg atccgtgaat
     5581 ggagatggat atcggtcagt ttttgcaggt acatcacgat gctctcccgc tgggcgtcgt
     5641 agtagtattt ctggatgaat gccgggaatt tctcctccag ccgggccggc tgtgcacctt
     5701 caggcatcag gatgtatgtc cagcagggat tccacaccca ggtctggggc aatcggccgt
     5761 tgaacacctt tttcatggtg ctgagcgaag cgatgaaatc aaaccggaaa tgggtctggg
     5821 ataccggctc tttcaccacg ccggtcactt tcaacggcag gttgacttca tacttcagta
     5881 tcttcccgta aggatcttca ccggggaaat atttctccgc catcttttcc gtaataacaa
     5941 ccgaatgagg ctccaggagc gccgtagcag gatccccttt gataaactcc tgtgagaaga
     6001 tttcaaagaa actcgaatcg gcaaaataga agcggctttc actgaatttc tttccttcat
     6061 attccaccag gctgcggtcc gactgaaaat tgaagatcct gacgacattt tccaccaggt
     6121 cgggatactc agatttcagt gtataggcta ccggaaaagg catgctggcc gaattctcgc
     6181 caacgccgcc gaagtcatag atcatgcaga cgcgggcaat ccggtcggag ttccggtaat
     6241 ggcggtcgaa gctcagttca tcccttacat aaagcatgat gaggatgaaa ctcgttaggc
     6301 caatcgcaag cccgagaagg tttattacgg tgtaaaccct tcgcttgacg aagccccgaa
     6361 gcaaaatcct tatgaaattg ttaaacattg actttccttc tgaacatcag cggtacgttc
     6421 tctccctgcg gagcgccgat ctccatcagt tcccagtctt tttcatgttc ggcgatctta
     6481 tgtttcagaa cggtgacttt atctttcggc ggaacgcctt ccgcttcgaa attgaccagt
     6541 tctaccctgt aatggatgac ctttttccgg ccattgatcg taacttcgat ctctacgttc
     6601 tgttccggct caaagtcggg aacaataatg acaatttcgc gcataaagaa gagtttttca
     6661 ggtttcagga ttccgggttc aattgttcaa ttgttcaatt gttctattgt taaactgtta
     6721 aactgttaaa ttgatgacca acgcctcacg accgccaact gccaactgcc aactgactgc
     6781 tcatctgagc accagcttat ccttctcccc gaacgaatca tacgacgaca cgatcacctc
     6841 ctcgccggct tccagtcctt cgagcacttc gtagaaccgg gtattttgcc ttcccagttt
     6901 aatatttctt ttcacggcat attttcccga aggatccacg acatagaccc agttgccgcc
     6961 ggtttgctgg aagaagccac cccgtttcag gatgagcgcc tcggtcgggg tgctgaactg
     7021 caaccgcatc tggatcgtct gtccccgctt gatgtttgcc ggtacctcgt cccggaacat
     7081 catatcgacc tggaaagccc cgcctgtcac cccggtgtaa atcttatata cgtagagctg
     7141 atacgttttt ccgccaaaat caagctctgc ttcctgcccg ggcacaacgc gcgacacata
     7201 ccgctcatcg atattcgccc gcatcttgta gccgtccat
//
LOCUS       DAOQXS010000072         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10732_length_7226_cov_3.478176, whole genome shotgun sequence.
ACCESSION   DAOQXS010000072 DAOQXS010000000
VERSION     DAOQXS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_7226_cov_3.478176"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKM34_03305"
     CDS             complement(<1..295)
                     /locus_tag="PKM34_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF479 domain-containing protein"
                     /protein_id="HNQ82643.1"
                     /translation="MNFLAHLYLSGKEEDVIIGNFVADAIKGNSLGHYPQGMERGIRL
                     HREIDSYTDSHELFRASRNRLAPRYNHFSGIIVDLYYDHFLAKFWNEYSNEDLR"
     gene            446..2071
                     /locus_tag="PKM34_03310"
     CDS             446..2071
                     /locus_tag="PKM34_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ82644.1"
                     /translation="MEKLLLLGDEAIAQSAIDAGLSGIYAYPGTPSTEILEYVHRSKE
                     AKENKIRASWSVNEKTAMEAALGMSYAGKRAMVCMKHVGLNVAADAFINSAITGANGG
                     LIVVAADDPSMHSSQNEQDSRFYGKFAMIPVLEPTNQQEAYDMVRAGFLLSEKYGIPV
                     LLRITTRLAHSRSGIIRREPLPQNEVHLPGNPKQFILLPANARRQYKSLLRNQVLFEE
                     ESESSDFNQYREGKDKSLGVITCGIAYNYLFENFQDREFPYPLLKIGQYPVPRKMIEK
                     LYSECNTILILEEGYPILEELLRGLLDDGKKIKGRMDGTVPRDGELNPNIVARAFGLD
                     EDYGFDVPGLVKSRPPSLCKGCPHIYSYNALNEALNLYSKGRVFSDIGCYTLGALEPY
                     GAINTCVDMGASITMAKGAADAGYVPAVAVIGDSTFTHSGMTGLLDAINDKSPVTVMI
                     LDNATTAMTGGQKSSATGRLEDICLGLGVEKEHIHILNPSPKNHEENLKIIEQEINYP
                     GVSVIIPRRECIQTVARRLRDEKKEKQKQEVIE"
     gene            2075..2659
                     /locus_tag="PKM34_03315"
     CDS             2075..2659
                     /locus_tag="PKM34_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNQ82645.1"
                     /translation="MKKDIILAGVGGQGILSIAAVIGMAALDSGLQLKQAEVHGMSQR
                     GGDVHSNLRISDREIFSDLIPFGKADLIISVEPMESLRYLPWLSKSGWLISNNVPHVN
                     IPGYPELASIESAIHAVGQHVLINADEMAKDLGMPRSANMVILGAASPWLGIDFSTLE
                     SAVRKIFGSKGADVVNQNLMALTAGKEFAMAHHS"
     gene            2741..4015
                     /locus_tag="PKM34_03320"
     CDS             2741..4015
                     /locus_tag="PKM34_03320"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNQ82646.1"
                     /translation="MKKRYLYGLLSLIVLGLSILLPYLMPDQKSHVTTEAQPESLSVE
                     QHIEYGINCDSLRVEKGIVRQNQSLSEILSFYDIGSEIIHQIASLSRPVFDVRKMRVG
                     NPYTVLMTKDTVPEVVYFIYEEDAVSYVTYQLKDELKVNKGAKELTREISTASGVIES
                     SLWNSLAENGKDPTLANEMSEVYAWTIDFFGIQKGDYYKVIYEELKVEGKPIGIGNVI
                     AACFHHMGSDLYAFRYTSNGETGYYDQKGENLQRAFLKAPLRFKRISSKFSHGRMHPI
                     LKIRRPHLGIDYAADYGTPVQTIGDGTVIEKGWNKGGGGNYIKIKHNGAYTTLYMHLA
                     GFAKGISTGTRVRQGDVIGYVGSTGLSTGPHLDFRVFLNGSAIDPLKMESPPSEPVAK
                     EYLPQYLPLVDSLKTGLDTLKVIRAQQLIVNR"
     gene            complement(4324..5754)
                     /locus_tag="PKM34_03325"
     CDS             complement(4324..5754)
                     /locus_tag="PKM34_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNQ82647.1"
                     /translation="MKTIRIFLYIAVASFLFACSEDKMDEINKERNDALEMAAKNILP
                     DIMLKTAFEVAGTDIPWYATVYIEHSAGTWAQSATADKRVAQNDASLFNNNWMSIYDV
                     LNACKAVIDKTDPVNGDESTNNWARGIAYVMTAYNLATATDMWGEVPWTEALQGAEEL
                     TPKYDKQSFLYTEIQRLLDSAIVNLGKTSIKYGAFDYIYGGDETKWIKAAWSLKARYY
                     LRLSQRDANAYDKALACIANGFTSNDDNFLFAAYESSASGENPWYQFLADRTHLSASK
                     TLYDLMNERVDPRIAAYFTQIDGAYNPAPNGTATESQGGVYSTSLLTENGQTAPTPLM
                     TYHELKFIQAECLLHKGDLGWQMAMGEAVAANFQWHGITDVQVVTDYLLNNIAPLAAV
                     DPLKELLTQKYIAFYEFEATEAYNDYRRTGVPSMNNPNNLTATGGFVNRFPYAISEVS
                     SNSENVPSIDVFKNKVWWAGGNELVE"
     gene            complement(5774..>7226)
                     /locus_tag="PKM34_03330"
     CDS             complement(5774..>7226)
                     /locus_tag="PKM34_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ82648.1"
                     /translation="NMANTSTQTASESKYWDRTVGFYGNLMVDWKSLLFLNLTGRMDN
                     VSSMPRGNRQFFYPSASLGFVFTELEGLKDNKILSFGKVRVSWAQVGQPGDFRQNVYV
                     LGGAGSGFLDDGILYPLGGISGYKPSNTIYDPDLTAQNTNTLEGGIELKFFQNRLGID
                     YSYADQTAEGQIFGVPLAGSTGYATYYTNAGKMTSKAHEIILNGTPVKTDNFTWDLLV
                     NFTKVKNVCEELAEGVESIFLGGYETPNIRASAGDTYPAIYGNLFARDEQGRILVDED
                     PNSYYYGMPYQGEFGKIGEVTPDFIMGLTSNMYIYKMFTLTFRADWKQGGDMYSGSNR
                     LMNLYGTSAVTEDRETPWVFNTDNGFRWDGYKADGTPTDIQRGGVEDFYAYPDLYNDV
                     FGNIEEAAVYETSFIKIREIVLSFDFPEKVIKSLRLQNLSLNLFTRNILLWTTLPNFD
                     PEASQGMGNMQGGMDYMSLPQTTTYGAGLNITF"
BASE COUNT         1807 a         1726 c         1846 g         1847 t
ORIGIN      
        1 ctctcaagtc ttcattcgag tattcgttcc agaattttgc caggaaatga tcgtaataca
       61 aatcgacaat gatcccggaa aaatgattgt agcggggtgc aagcctgttt ctgcttgccc
      121 tgaaaagctc atggctgtcg gtatagctgt ctatttcgcg atgcagcctg atcccccttt
      181 ccataccttg aggataatga ccgagggaat tgcctttgat tgcatcggcc acaaaattac
      241 cgatgatgac atcttcttct tttcctgaaa gataaaggtg agcgagaaaa ttcatataaa
      301 atcaacgtga ttattttgaa aatattttat gcttttatct gctaatttat aatgattgta
      361 gataagcccg acggcattcc cctttctgaa aattattcta aatttgtcag tgaggctacg
      421 aatatctttt atgtagagaa ttattatgga gaaattgcta ctgctgggcg atgaagcgat
      481 tgcacagtct gcgatagatg caggtttatc aggaatttat gcctatccgg gaacaccttc
      541 cacggagatc ctggaatatg tccaccggtc gaaagaagca aaagagaata aaatacgggc
      601 cagctggtcg gtaaatgaga aaacggccat ggaagcggca ctgggaatgt catatgcagg
      661 caaaagagct atggtatgta tgaagcatgt tggactcaat gtggctgcgg atgcctttat
      721 taactcggct attaccgggg caaacggtgg ccttatcgtt gttgcagcag atgatccttc
      781 catgcattcc tcacaaaatg aacaggatag ccgtttttat ggaaaatttg ccatgatccc
      841 ggtgctggaa cccaccaacc agcaggaggc atacgatatg gtgcgggccg gattcctgtt
      901 atcggagaaa tacgggatcc cggtccttct caggatcacc acacgcctgg cccattcgcg
      961 ttcaggtatt atccgccggg aacccctgcc acaaaacgag gttcatcttc cgggcaaccc
     1021 caaacaattt atccttctcc cggccaacgc caggaggcaa tataaatcct tgctgcgtaa
     1081 ccaggttttg ttcgaggaag agtcggaaag ttcggatttc aatcagtacc gcgaaggaaa
     1141 agataaaagc ctgggcgtaa ttacctgcgg gattgcttac aattatcttt tcgagaattt
     1201 ccaggacagg gagtttcctt atccgttgct caagatcggt cagtaccctg tcccccggaa
     1261 aatgattgag aagctttaca gtgaatgtaa taccattctt attctggaag aaggatatcc
     1321 gatcctggag gagcttttga gaggtcttct ggatgatggt aaaaagatca aaggaaggat
     1381 ggacgggacg gttccgcgcg acggggaact gaatccaaat atcgtagccc gtgcattcgg
     1441 gctggatgag gattatggct tcgatgtacc cgggctggtg aagtcgcggc caccttcact
     1501 ctgcaaagga tgcccgcata tctattccta caatgccctg aatgaggcgc tgaatttgta
     1561 ttccaaagga agggtattca gcgatatcgg ttgctatacg ctcggggcgc tggaacctta
     1621 cggcgccatc aatacctgcg tggatatggg cgcttcgatc accatggcca aaggggccgc
     1681 cgatgccggc tatgttcccg ctgttgccgt gatcggcgat tcgacattca cccattccgg
     1741 catgaccggc ctgctcgacg ctatcaacga caaatccccg gtcacagtca tgatcctcga
     1801 taatgccacc acggcgatga ccggcgggca gaaatcgtcg gctacaggca ggctggagga
     1861 tatttgcctc ggcctgggag tggaaaaaga gcatattcac atcctgaacc catcccctaa
     1921 gaatcatgaa gagaacctga aaatcataga acaggagata aattacccgg gcgtctcagt
     1981 cattattcca cggcgcgaat gcattcagac cgttgcacgg cggctccggg atgaaaagaa
     2041 agaaaagcag aaacaggaag tcatcgaata gaatatgaag aaagatatta tccttgcagg
     2101 ggtcggtggc cagggtatcc tgtcgatcgc agcagtgatc ggaatggcag ccctggattc
     2161 cgggcttcaa ttgaaacagg ccgaagtgca cggcatgagc caacggggtg gggatgtcca
     2221 ctccaacctc cggatctccg accgtgagat tttctccgat ctgatcccat tcgggaaagc
     2281 agacctgatc atctccgtcg aaccgatgga atcactgcgc tacctgccct ggctgtcgaa
     2341 aagcggctgg ctcatttcca ataatgtacc tcatgtcaat atccccggtt atcctgagct
     2401 tgccagtatc gaatcggcta ttcatgccgt cggtcagcat gtgctgatca atgccgatga
     2461 aatggcgaaa gatctcggaa tgccccggtc ggcgaatatg gtgatcctgg gggctgcttc
     2521 tccgtggctc ggtattgatt tttctaccct cgagtcggca gtcagaaaaa tattcggctc
     2581 caaaggagcg gatgtggtga accagaacct gatggcctta acggctggaa aagagttcgc
     2641 tatggcacac cattcctgag aatcatggaa aggccgttaa attatcggac catcctttca
     2701 agaaaaattt tatttttggc cttccttgaa gaataaattc atgaagaaaa gatatctcta
     2761 tggcttgctg tcattaatcg ttctggggct ttccatcctc cttccttatc tcatgccgga
     2821 ccagaaaagc catgttacga ctgaagcaca gcctgaatca ctttccgttg aacaacatat
     2881 tgaatacggt atcaactgcg attccctgcg ggttgaaaag gggattgtca ggcaaaacca
     2941 atcgctctcg gagatcctct ctttttatga tatcggcagc gagatcatac accagatcgc
     3001 cagcctttcg cggcctgtgt ttgatgttag gaagatgagg gttggaaacc cttatacggt
     3061 tttgatgaca aaagacaccg ttccggaggt ggtttatttt atttatgaag aagatgcggt
     3121 ttcctacgta acgtatcaat taaaggatga gctgaaggta aacaaaggcg ccaaggagct
     3181 gacccgcgag atcagcaccg ccagcggcgt tatcgaatct tccctctgga attccctggc
     3241 cgaaaatgga aaagatccga ccctggccaa tgaaatgtcg gaagtttatg catggacgat
     3301 cgatttcttt ggtattcaaa aaggagatta ctataaggtg atctatgaag aactgaaagt
     3361 agaaggaaaa ccgataggga tcggaaatgt cattgccgcc tgtttccacc atatggggtc
     3421 ggacctctac gcttttcgtt acacaagtaa tggagaaaca ggttattatg accagaaggg
     3481 ggagaacctt cagcgtgcat ttttgaaagc ccctcttcgt tttaagcgaa tcagttccaa
     3541 gttttcgcat ggccggatgc atcctatcct gaagatcagg cgtccgcacc tgggaattga
     3601 ctacgctgcc gattacggca ccccggtgca aaccattggc gacgggacag tgatagaaaa
     3661 aggatggaat aaaggaggcg gaggtaatta catcaagatc aagcacaatg gcgcctatac
     3721 cacactctat atgcacctgg ccggatttgc caaaggaatc tcgaccggaa cccgggtacg
     3781 ccagggcgat gtgatcgggt atgtcggcag caccggcctc agcaccgggc cgcatctcga
     3841 tttccgcgtt tttctcaacg gcagcgccat cgacccattg aaaatggaat cacccccttc
     3901 agagcccgtt gcaaaagagt acctgcccca atacctgcca ttggtggatt cactgaaaac
     3961 cggactcgat accttgaaag tgatccgtgc gcaacaactc atcgtgaacc gttaatccct
     4021 tttatcgtct ctgttattat tgctgaggct gtctcaaaag gctttttatt aaccactaag
     4081 gcgcacaaag tgcatcacga aggaacacta agtagttgaa tatcaattaa tatacctttg
     4141 tacgcctttg tgaattcctt tgtgttcctt tgtggttaaa tccttttgag acagcctcct
     4201 ccagaattaa tcgaaccgac ggattcacct gaccggactg ccatgtttga tcgtcgtcag
     4261 acttccaaaa aaaagagggt gcgctccgtg gaggcaccct ctaattatta aaataaatat
     4321 ctactattct accagttcat tgccaccggc ccaccagact ttattcttga atacatcgat
     4381 ggaaggcaca ttttctgaat tggaagatac ctcgctgatc gcatagggga aacggttgac
     4441 gaatccaccg gttgcagtca ggttgttcgg gttgttcatc gatggaacgc cggtgcggcg
     4501 gtagtcgttg taagcttccg tagcttcaaa ctcatagaag gcaatatact tctgggtaag
     4561 aagctctttc agcggatcaa cggctgccag cggagcgata ttgttcagca ggtaatcggt
     4621 cacaacctgt acatcggtga tgccgtgcca ctggaagtta gcagcgacag cttcgcccat
     4681 tgccatttgc cagccgaggt cgcctttatg caacaagcac tctgcctgga tgaacttcag
     4741 ttcgtgatag gtcatcagcg gagtaggggc ggtttggccg ttttccgtca agagtgaagt
     4801 ggagtatact ccgccctggc tttcggttgc agtaccgttc ggagccgggt tataggcgcc
     4861 gtcgatctgc gtgaagtatg cagcaattct cgggtcgacg cgttcgttca tcaggtcata
     4921 cagcgttttg ctggcagata gatgggtacg gtcggccagg aactgatacc acggattttc
     4981 acccgatgcg gatgattcgt atgcggcaaa gaggaaattg tcatcattgc tggtaaatcc
     5041 gttggcaata catgccagcg ctttgtcgta agcattagcg tccctttggc tgagtctcag
     5101 gtaataccgg gctttcagcg accaggcagc cttgatccac ttggtttcat cgccaccata
     5161 gatatagtcg aatgcaccat atttaatgga ggttttgccc aggttaacaa tggcagagtc
     5221 gagtaaccgc tgaatttcgg tgtagaggaa tgattgtttg tcgtacttgg gcgtgagttc
     5281 ttctgctccc tgcaaagctt ccgtccaggg tacttcaccc cacatatcgg ttgcggttgc
     5341 aaggttgtat gccgtcatca cataagcgat acctctggcc cagttgttcg tcgactcatc
     5401 gccgttaacc ggatcggttt tatcgataac ggctttacag gcgttgagga catcgtagat
     5461 gctcatccag ttattgttga acagggaggc gtcgttctgg gccacccttt tgtcagcggt
     5521 tgccgactgt gcccaggttc cggcgctatg ttcgatgtat acagtggcat accagggaat
     5581 atcggtaccg gccacttcaa aggcggtttt cagcatgatg tccgggagga tgttcttagc
     5641 ggccatctcc agggcatcgt tgcgttcctt gttgatttca tccatcttgt cttcactgca
     5701 ggcaaagagg aaggatgcaa cggcgatgta aaggaatatt cttattgttt tcataatcat
     5761 tactatttac aggttagaaa gttatgttaa gaccggcgcc gtatgtcgta gtttggggca
     5821 gggacatata gtccatcccg ccctgcatgt tgcccatacc ttgtgaagct tcgggatcga
     5881 aattcggcag ggtggtccaa aggaggatgt tcctggtgaa caggttcagc gacaggttct
     5941 gcaaacgcaa tgatttgatc accttttcgg ggaagtcgaa tgagagaacg atctccctga
     6001 tcttgatgaa ggaggtctcg tacacagcgg cttcttctat atttccgaag acgtcgttgt
     6061 acaggtcagg ataagcatag aaatcttcca cgccacccct ctggatgtcg gtcggggttc
     6121 cgtcagcttt gtaaccgtcc cagcggaatc cgttgtcggt attgaatacc cacggggttt
     6181 cgcggtcttc ggtaacggcc gaggtgccat acaggttcat caatcggttg gagccggagt
     6241 acatgtcgcc accctgcttc cagtctgcgc ggaaagtgag ggtgaacatc ttatagatat
     6301 acatgttgct ggtgaggccc atgataaagt cgggggtcac ttcgccgatc tttccgaatt
     6361 cgccctggta cggcatgccg taatagtagc tgttcgggtc ttcgtcgacc aggatacggc
     6421 cctgttcgtc acgtgcaaac aaattgccat agattgcggg ataggtatca ccggcagaag
     6481 cacggatatt cggggtttca taaccgccaa ggaaaatgct ctccacgcct tcagccagtt
     6541 cctcgcacac attcttcacc ttggtaaagt tcaccaacag atcccaggta aagttgtctg
     6601 ttttaaccgg tgttccgttc agaataattt cgtgtgcctt ggatgtcatt ttaccagcat
     6661 tggtatagta tgtagcataa ccggttgatc cggccagcgg tacgccgaaa atctgtcctt
     6721 cagctgtctg gtcggcatat gaatagtcaa ttcccagtct gttctggaag aacttcagtt
     6781 cgataccgcc ttccagagta ttggtgttct gagccgtcag atccggatcg tagatggtgt
     6841 tgctgggttt gtaaccagaa ataccaccga gcgggtaaag gatgccgtcg tccaggaatc
     6901 cgctgccagc cccgccgagg acatagacat tctgcctgaa atcgccgggt tgtccgacct
     6961 gcgcccagga gacgcggact ttaccgaagc tgaggatctt attgtctttc aggccttcca
     7021 gttcggtaaa cacgaatccg agtgatgcgg aaggatagaa aaactggcgg ttgcccctgg
     7081 gcatcgatga cacattgtcc atacgaccgg tcaaattgag gaacaacaag ctcttccagt
     7141 caaccatgag gttgccgtag aaaccgaccg tcctgtccca atacttcgat tcggaagcgg
     7201 tctgtgttga tgtgttggcc atattg
//
LOCUS       DAOQXS010000073         7213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10756_length_7213_cov_3.342973, whole genome shotgun sequence.
ACCESSION   DAOQXS010000073 DAOQXS010000000
VERSION     DAOQXS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10756_length_7213_cov_3.342973"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..4104
                     /locus_tag="PKM34_03335"
     CDS             10..4104
                     /locus_tag="PKM34_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ82649.1"
                     /translation="MKANLNIIRIAAWFILAGLFLINPVFSQKITYSDPLNKAGFSLG
                     QETRGGVTVNYTIETFTFSPVDINGKGMKNVELPGNFLPGDAGAPNLPGSGRYIAFPK
                     GAVPVLTIVSQRTESFKNVDIAPSPEIPLDTDREPLKYPVRTDIYTTNAYYPATPVQL
                     SNPTQIRGVDVVMLGVTPFQYNPVTKELIVYRDLEIRVDFQGGSGQIGEERLRSRFWD
                     PILEDAVLNHKSIPAVDYSRRVLNPNRSNNGGDYLIIVANSDDYMNWAQEIRSFRIKQ
                     GIVTEIRTLEDVGGNSPAIIENFIDDVYENWDPVPAAILLMGDYGSNAEVNITSPIWD
                     SYCVSDNIYGDVDEDDMPDIVMARMVGNNNTEMETFATKFINYETNPPTNPSFYDHPI
                     TALGWQTERWFQICSETVGGYFKNVQGKNPVRINEIYQGNPGSDPWSTATNTQVILDE
                     FGPNGLGYIPATPQELGNWSGGNATAINNAINSGAFLLQHRDHGEETGWGEPQYGNGN
                     IGGLTNTDLTFIFSINCLTGKYNWSGECFSEKFHRYRYNNVNSGALGVICASEVSYSF
                     VNDTYVWGLFDNMWPDFMPEYGSTPAERGLLPAFGNAAGKYFLKASSWPYNSENKEVT
                     YNLFHHFGDAFQTLYSEVPQEMTILHDGIILAGLNEFTITADAGSTICLTVDDDIIGL
                     ATGTGNPQVITITPLSVGTTVTLTVTKTNYFRHEEDIEVIPAEGAYCLYGNHTVADGI
                     GGNGNGLVDYNETVGLSLGIRNVGMSDGDGVTVTISTLDPFIAIEDNTEFYGVIPSGQ
                     TVIVDQGFSFYAAENTPDQHQVLFSIVSTDGGNVWNSQFMLKVNAPVLNINAVTINDS
                     ENGNGNGILDPGEQAEMTVNYTNTGHATAYDVDVFMEGQSGYIEISNPEQNFASIGFL
                     GVFNKTFDVSVDEITPIGININFVNDLSMGNFYSSKTFVQKVHPLIEDFETGNFNEYN
                     WQFTGNLPWTTSNQYPYEGNFSALSGAISHGQTSEMKISYEVMSADSISFIRKVSSEA
                     TDKLQFYIGNTLAGEWSGTNEGWRREAFAVTPGYKTFRWVYTKNASGSGGVDKAWLDN
                     IHLPSPVALTIWAGPDEDICTGEEFQISGSYGTDYNTIEWTTSGTGTFTDNTQMQPVY
                     SPSSEDFNNGSVVLTLTLTGDGDLIVSDEMGLTFNAGPEAPQAIEGPDYVDLFITTTS
                     EYLTAGIEGITVYTWYLEPTDAGTIEGSGLQSAVTWNPVFAGTAYISVSGTDECGEGD
                     VSEAFEVTVGNSVGYSEDENSFGLSVYPNPGNGSFQVMIATPEHNAVTLKVINILGEV
                     VYKSDQKVNGAAIIPVNIENLANGVYLLSLEGDGISLNRKIVKR"
     gene            complement(4478..>7213)
                     /locus_tag="PKM34_03340"
     CDS             complement(4478..>7213)
                     /locus_tag="PKM34_03340"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNQ82650.1"
                     /translation="YFGWITCDPATGTLSMIFYDDRNVSSSQVEVFCANSIDAGDTWE
                     DFKVSDVAFSPSPLPGYASQYFGDYLGISARNGKVYPVWTDNRSGTALAYTSPYVTST
                     MMPPTALNAQLNDETGQVNLTWTHEAGPTFDHYNIYRGFSLIGTTVLPIYSDMLPGYG
                     SYRYQVTAVYIIEGESAPAIADVQWGDAQAETDPASIEVFVLPESTSSFIMDLANTGE
                     LPLEYTSTFSLPGNAREAGLAYCTGIGGCGGEGIAGVVYGDVSNFSECNSYSDYSDLS
                     HMVSLGDSFEITVQNITNMYSEDVCGIWVDWNGNESFLDDGAITVSGSPGPGPYTATI
                     TVPDNAANGEVRMRIRIRRGGTLSPCGSAPNGEVEDYSLNVLGWVTANPREGEIDAGE
                     TQPVTFDFNSAGLALGTYLAHFTIISNDPDNGELVVPVTMHVDNAAVTITSDKDSLCL
                     GSSTTLHANITGGSGNFTYSWTSDPAGFVSDDPDPVIEPTVTTTYFVEATDGNIILSD
                     QITITVLAIPDLNLGNDLFACVNDEVVLDAGAGFQQYFWSTGETTQSITVTGPGSYWV
                     TVLNENECAATDTVNVIFNPLPEISLGPDQQFCQGTTVMLSAGTGFQSYLWNTGASSY
                     YINAGEPGEYWVEVTDNNGCRNRDTITLAVLPRPEVDLGEDRIFCEGTTVLLTPGSGY
                     EGYLWSNGATSASIDAGLPGDYWVEITDETGCKNTDTVTLIMDPLPLAPQITSGPQSV
                     DLYVILVSDYTLAESAYADSYEWNISPAAAGSVDGNGTTAQVTWNTGFTGTADITAKG
                     VNDCGNGNDSQVYSVSVYSSQGIGEPGAISSVKLFPNPNEGNFVLEMNSKEEQELIFS
                     VASAGGNKVLETGEKLGKGVIKKSFNLSAQPAGTYYITISDKENKILGRLQVVIK"
BASE COUNT         1853 a         1752 c         1843 g         1765 t
ORIGIN      
        1 ttacactaaa tgaaagctaa tctgaacatt atcagaatag ccgcatggtt tattttagcc
       61 gggctattcc tgatcaatcc tgttttttcg caaaaaatca cctattccga tcccctaaac
      121 aaagccggat tttctcttgg gcaggaaacc cgcgggggtg ttacggtaaa ttacactatt
      181 gaaactttca cgttctcacc ggttgatatc aatggtaagg ggatgaagaa cgtggagctg
      241 ccgggaaact tcctgcccgg tgatgccggt gcgccaaacc tgccgggcag cgggcgttat
      301 attgcttttc caaaaggtgc ggtaccggtt ctgaccattg tatcgcagcg taccgaatcg
      361 tttaagaatg ttgatattgc accatcgccg gagataccgc tcgatacgga ccgggaaccg
      421 ctcaaatacc ctgtaagaac tgacatttat actaccaatg catattatcc ggccacaccg
      481 gtgcagctct ccaacccgac tcaaatcaga ggggtggatg tggtgatgct gggtgttacc
      541 cctttccagt ataatcctgt aacaaaagaa ctgatcgttt accgtgacct tgagatccgg
      601 gtcgatttcc aggggggtag cggacagatc ggtgaagaga ggctgaggag ccgtttctgg
      661 gaccccatcc tggaggatgc cgttctgaat cacaaaagca ttcccgccgt tgattacagc
      721 aggcgcgttc tgaatccgaa caggagcaac aacgggggtg attacctcat tatcgttgcc
      781 aacagcgacg attatatgaa ttgggcccag gagatcaggt cgttcaggat aaagcagggt
      841 attgtcaccg aaatcaggac gctggaagat gtcgggggaa acagtcccgc tattatcgag
      901 aattttatcg atgatgttta tgaaaactgg gatccggtcc cggccgcaat attgctgatg
      961 ggtgactacg gcagcaatgc cgaagtgaat atcacttccc cgatctggga cagctattgt
     1021 gtttcggata atatctacgg tgatgtggat gaggatgata tgcccgatat tgtaatggcg
     1081 agaatggtcg ggaacaacaa caccgaaatg gagactttcg ccaccaaatt tatcaattat
     1141 gaaaccaatc cgccgacaaa tccctcgttc tatgatcatc cgatcacagc actgggatgg
     1201 cagacggaac gctggttcca gatctgttct gaaacggtgg gaggctattt taagaatgtg
     1261 cagggaaaga atcccgtccg gatcaatgag atttaccagg gcaacccggg ttccgatccc
     1321 tggtcgactg caaccaatac gcaggttatt ctggatgaat tcggtccgaa cgggcttggt
     1381 tatattccgg caacaccgca ggaactggga aactggtcgg gtggcaatgc tacagcaatt
     1441 aataacgcga tcaacagcgg cgccttcctg cttcagcacc gcgatcatgg cgaggagacc
     1501 ggctggggag aaccgcaata cggtaatggc aatattgggg ggctgaccaa caccgacctc
     1561 acctttattt tttccattaa ctgcctgacg gggaaataca actggtcggg agaatgtttc
     1621 tcagagaaat tccaccggta caggtataac aatgtgaaca gcggcgccct gggagtcatt
     1681 tgcgcttccg aagtaagcta ctcatttgtg aatgatacct atgtttgggg attgttcgac
     1741 aacatgtggc ccgattttat gcccgaatac ggttcaacgc ctgcagagcg ggggcttctt
     1801 cccgcttttg gcaatgcggc cggcaaatac tttttaaaag cttcgagctg gccctacaat
     1861 tctgagaaca aagaggtgac ttacaacctg ttccaccact tcggcgacgc tttccagacc
     1921 ctctattcgg aggtgcccca ggagatgacc atcctgcacg acggcataat cctggccggt
     1981 ctgaatgaat ttaccattac agccgatgcc gggtcaacca tctgcctgac ggtcgatgac
     2041 gatattatag gcctggcaac cggaacgggt aatccgcagg taataaccat taccccgtta
     2101 tcggtaggaa ccacggtgac gctgacggta accaagacca actacttccg gcatgaagag
     2161 gacatcgagg tgatccctgc cgaaggagct tactgcctct acggtaacca tactgtggct
     2221 gatggcattg gcggtaacgg caacggcctg gtggattaca atgaaaccgt cggcctgagc
     2281 ctgggaatcc gcaatgtcgg tatgtcagac ggcgatggtg ttacggtaac catctctacc
     2341 ctggatccct ttatcgctat tgaagataat acagaatttt acggagttat tccttccgga
     2401 cagaccgtga ttgtagatca aggattttct ttctatgccg cagagaacac cccggaccag
     2461 caccaggtcc ttttcagcat agtttcaacg gatggcggaa atgtctggaa tagccagttt
     2521 atgctgaaag ttaacgctcc tgtactgaat atcaatgcgg tgacgataaa cgattcggaa
     2581 aacggcaatg gcaacggaat tctcgatccg ggcgaacagg cagagatgac ggtgaattac
     2641 accaacacag gacatgccac ggcctatgat gtggatgtgt tcatggaagg tcaatcggga
     2701 tatattgaga tttcaaaccc ggagcaaaat ttcgcctcga taggttttct gggggttttt
     2761 aataagactt ttgatgtatc ggtcgatgag ataaccccga tagggatcaa tatcaatttt
     2821 gtcaacgacc tgtcgatggg taatttttat tcttcgaaga ctttcgttca gaaagttcat
     2881 cccctgatcg aagattttga gaccgggaat ttcaatgaat ataactggca gtttacgggt
     2941 aatctgccct ggacaaccag caatcagtac ccgtatgagg ggaatttcag cgcattgtcg
     3001 ggtgcgatct cgcatgggca aacctctgag atgaagattt cttacgaggt aatgtcggcc
     3061 gatagcattt ccttcatccg caaggtttct tccgaagcaa ccgacaaatt acagttctat
     3121 atcgggaaca cgctggcggg agaatggtcg ggaacaaacg aaggctggag gcgggaagcc
     3181 tttgccgtaa cgccgggata caagacattt cggtgggttt ataccaagaa tgcctccggg
     3241 agcggcggcg ttgacaaagc ctggctggat aatatccatc tgccatctcc ggtagcgctg
     3301 accatctggg ccggccccga tgaagatatc tgcaccggcg aagagttcca gatctcaggt
     3361 tcttatggca cggattataa caccattgaa tggacaacca gcggtacagg cacatttacg
     3421 gataataccc agatgcagcc ggtatactcg cccagctcag aagatttcaa caacggaagt
     3481 gttgttctga cgctcaccct gacaggtgac ggggatttaa tcgtatcgga tgaaatgggg
     3541 ctgaccttca acgccggccc ggaagccccg caggccattg aaggccccga ttacgtagac
     3601 ctgtttataa ctacgacatc tgaatacctg acagcgggta ttgaaggcat tacggtctat
     3661 acctggtacc ttgagcctac tgacgccggt acaattgaag gatcgggcct ccagtcagcg
     3721 gtgacctgga atccggtatt tgccgggact gcttatatca gtgtttccgg taccgatgaa
     3781 tgcggcgaag gtgatgtttc ggaagctttt gaagtgactg ttggaaattc tgtgggttac
     3841 tctgaagatg agaacagctt cgggctgtcg gtttatccga atcctggcaa cggcagtttc
     3901 caggtgatga ttgccactcc ggaacataac gctgtgaccc tgaaagtgat caatatcctg
     3961 ggagaagtgg tatataagtc ggatcagaaa gtgaacggtg ccgccattat cccggtgaat
     4021 attgagaacc tggccaacgg tgtctatctc ttatccctgg aaggagatgg catctcattg
     4081 aacagaaaga tcgtcaagag gtaaaaatat caaatctgaa actgaagatt gggtgtctca
     4141 aaagttcttc gtgcaacttc gtgaaacctt cgtaaaattt ataaataatt gttacacaga
     4201 gttccacgaa gaaggcacta agatatacga agttttttga aagaaatctt actttttcag
     4261 acaccctctt ttttgggggg gtagtccgac gactgaagcc gtcggttccc accttcttaa
     4321 aaccattgga accaacggct ttagtcgttg gagttcgatc acaggcaata gacgccttta
     4381 gttatcgaat tgcccggaaa agcgtcggac tacaagtaaa tatgatgcca tgcagacaaa
     4441 taaaacatta ttctgctgac agttatttta tgacgattca ttttattaca acctgcaaac
     4501 ggccgagtat tttattttcc ttatcgctga tggtgatgta gtatgtgccg gctggctgtg
     4561 ctgaaagatt gaaactcttt ttaatgacgc ctttaccgag cttttctccg gtttccaata
     4621 ctttgttccc tccggctgat gctacgctga agatcagttc ctgttcctct tttgaattca
     4681 tttcaagaac aaaattacct tcgtttggat tcgggaatag cttgaccgaa gagatggccc
     4741 caggctcgcc aataccctgt gatgaataaa cagaaacgct ataaacctgc gaatcatttc
     4801 cattcccgca atcgttgaca ccttttgccg ttatatcagc cgtaccggta aaacctgtat
     4861 tccaggttac ctgggcggtt gtcccgttgc catcgacaga acctgcggca gccggggaga
     4921 tattccattc atatgaatca gcataagcgc tttcggcaag ggtataatcc gaaacaagta
     4981 tgacgtaaag atcgacagac tgaggcccgg aggttatttg cggggccaat ggaagcgggt
     5041 ccattatcag ggtgacggta tcggtattct tacagcccgt ctcatcagta atttccaccc
     5101 agtaatcgcc cggcaggccg gcatcaatgg atgcggaagt agcgccattg ctccagaggt
     5161 agccttcgta gccgcttccg ggagtcagta acaccgtggt gccttcgcag aaaatccggt
     5221 cttcgcccag atcaacttcc ggccggggaa gaacggccag cgtgatcgtg tccctgttac
     5281 ggcatccgtt attatccgtt acttccaccc agtattcccc gggttcgccg gcattgatgt
     5341 agtagcttga agcgccagta ttccaaaggt acgactggaa cccggttcct gcggatagca
     5401 tcaccgtcgt cccctggcag aactgctggt cgggaccgag actgatttca gggagaggat
     5461 tgaaaataac attcaccgta tcggtggcag cacactcatt ttcattgagc accgtgaccc
     5521 agtaggaacc cggaccggta accgtaatcg attgagtcgt ttcacccgta ctccagaagt
     5581 actgctgaaa accggcaccg gcatccagta cgacttcatc atttacgcag gcaaacagat
     5641 cattgcccag gttcaggtcg ggaatggcca gtacggtaat ggtaatctgg tcggatagaa
     5701 tgatatttcc atcggtagct tcaacaaaat aggttgtagt tacagtgggt tcaataacag
     5761 gatccggatc atccgatacg aaccctgccg ggtcggaggt ccaggaatag gtaaaattac
     5821 ctgatccacc ggtgatattg gcatgcagag tggtggaact gcccaggcag aggctgtctt
     5881 tatcactggt aatggtcacc gctgcattat ccacgtgcat cgtgaccgga accaccagtt
     5941 cgccgttgtc cggatcattg ctgatgatgg taaagtgggc caggtaagtt cccagggcga
     6001 gccctgccga attaaaatca aaagtaaccg gctgggtttc acctgcgtca atttcccctt
     6061 ccctgggatt tgcggtcacc catcccagga cattgaggga gtaatcctcg acttcaccat
     6121 ttggcgcaga gccacagggg cttaatgtgc ctcctcttct gatcctgatc ctcattcgga
     6181 cctccccgtt ggcggcatta tccggaacgg taatcgttgc cgtgtaaggc ccggggcccg
     6241 gagatccgga taccgtgatg gcgccatcgt caagaaagct ctcatttccg ttccagtcga
     6301 cccagatacc gcatacatcc tcagaataca tgttcgttat attttgtacc gtaatctcaa
     6361 aagaatcccc gagtgaaacc atgtggctga ggtcggaata atccgaatag ctgttacatt
     6421 ccgagaaatt gctgacatca ccgtaaacaa ctccggcaat tccttctcct ccgcagcctc
     6481 cgattccggt acaatatgcc aggccggctt ccctggcatt gccgggaagg ctgaaagtcg
     6541 aggtatactc caacggaagt tccccggtat tggccaggtc catgataaaa gaagaggttg
     6601 attcaggaag gacaaatact tcgatggaag ccggatcggt ctctgcctgg gcgtcgcccc
     6661 actgaacatc ggcaatagcc ggagccgatt ccccttcaat aatatagaca gccgtaacct
     6721 ggtacctgta cgacccatag ccgggcaaca tatccgaata gatgggcaat accgtggttc
     6781 cgatcaatga aaagccgcgg taaatattgt aatgatcaaa tgtagggcct gcttcatgcg
     6841 tccaggtgag gttaacctgc ccggtttcat cgtttagctg tgcattcagg gcggttggcg
     6901 gcatcattgt gctggtcaca tatggcgaag tatatgccag cgctgtgcct gaacggttat
     6961 cggtccagac agggtaaacc tttccattcc tggctgatat acccaggtaa tctccaaaat
     7021 actggctggc ataaccggga aggggagaag gggagaaagc cacatcactg actttaaaat
     7081 cttcccaggt atctcctgcg tcgatggagt tagcacagaa aacctcgacc tgtgagctgc
     7141 tcacgttgcg gtcgtcgtaa aagatcatgc tgagcgtccc tgtagcagga tcacaggtga
     7201 tccatccgaa ata
//
LOCUS       DAOQXS010000074         7174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10831_length_7174_cov_3.871752, whole genome shotgun sequence.
ACCESSION   DAOQXS010000074 DAOQXS010000000
VERSION     DAOQXS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10831_length_7174_cov_3.871752"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKM34_03345"
     CDS             complement(<1..661)
                     /locus_tag="PKM34_03345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ82651.1"
                     /translation="MRKIFILSVFATFFTLAGFSQDTVMMQPADSLALADSTLLINHL
                     SDSAAKKIITVYDDYWVTNSIKCGWKTTDTALIGTGLELCDTAHPFVRPTIGKLWRGC
                     TYYHAGWDIGLDYGSPVLAGLNGKVRYARYCSGYGNLVIVRHYSGMEVYYAHLSKIRV
                     KTDQWVEPGDTIGLGGATGRARGNHLHMEFRICDRALDIADMYVHNDTVVNLYKIKEL
                     ST"
     gene            complement(939..1808)
                     /locus_tag="PKM34_03350"
     CDS             complement(939..1808)
                     /locus_tag="PKM34_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ82652.1"
                     /translation="MLQITTKTIYFERINKVLVYIDRNLGEKMDLEMLASMAHFSPFH
                     FHKIMRAYLRESLGSYIIRVRLDTAASLLIYSREPVSDIAYRIGYDTPAAFTKAFQKR
                     FGTSPSDYRQNKGSLRLEKVELSTFNFKAMNLKPKIKEIKPIPVVYVHAIGDYNKVGP
                     AWEKLAKFMKEKKLFSFGMDFIGVSYDDPSVTETEKLRYDACVTVKKEIRPEGEVGYK
                     VLEGGLYAIFRHTGPYENFSKTYDQIYLNWLPESGYELRDEPPLEFYLNDPDRTRPEN
                     LKTDIYVPVKREA"
     gene            2277..2948
                     /locus_tag="PKM34_03355"
     CDS             2277..2948
                     /locus_tag="PKM34_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82653.1"
                     /translation="MKTLSFNDKAKHLLILITLLVICLKMMAVTPEEEFRLMKQKNGI
                     DLFYRWMVMPEGNRVRQMKAVFEGGWTKEDIMYLLKNEQQAMAWIPSAREYRNLKEAT
                     AGEWFSYIRFSAPWPLADQDCILQYSLSTEPSGESTIRFTCRPDYIPEVDGITRMKDI
                     NGSFVICPGSGEKGRVECYFISSKASVIPRWITDPIITGSIISLMEGMRGQLSGVEYS
                     QTLQK"
     gene            2945..3406
                     /locus_tag="PKM34_03360"
     CDS             2945..3406
                     /locus_tag="PKM34_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNQ82654.1"
                     /translation="MKKLTFNPVTLAVNKLKSIKSDPEKVCYGFAFGTFISATPFIGF
                     KWLVALPIVWLAKWNKTACMIGILQVNYLTGPFFYALAYFVGKSVCGYENTFELPDRM
                     TFGAVKDIFFGNADVFVSLLAGGLLLSIPMTLGAFYLARSIFYRKLNTVMS"
     gene            3403..4200
                     /locus_tag="PKM34_03365"
     CDS             3403..4200
                     /locus_tag="PKM34_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ82655.1"
                     /translation="MKTQETYTLITGASMGLGRELATECARRGQNLLLVALPGSGLSE
                     LRDELKEDFRHHVECYECDLSHEAELEKLAAWVTENYSVDRLINNAGIGGTLRFEEAD
                     AGYLDTIIHLNIRATTILSRLLLPELKRHPSALIMNISSLAAFGPLPYKTIYPASKAF
                     IYSFSRGLSRELIDTGVKVVVVAPGPFISNPDVARRIMKQSFIARLGVLSAREITLKA
                     LKGAEKGQNVIIPGIWNRLNRLLMQLVPEDIRLSVLSNVLKRELPLK"
     gene            4210..4920
                     /locus_tag="PKM34_03370"
     CDS             4210..4920
                     /locus_tag="PKM34_03370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNQ82656.1"
                     /translation="MEPDTIKAVYAILATAAVSVAYYFVLYRIGKMKKPGTCSVAIGC
                     YLLRKITGFLVLGVVPVAVAWFVFGQSLAESGFRAGTTVTWLPYTALLALFLTALNYF
                     NSSNIRLQKAYPELRLENWGPGSALVLAFGWILYLVGYEMLFRGLLLHTCLEAFGLWP
                     AVIINLSLYFILHLPKGLKESVATIPFGAVVIFLTVKSGSVLPAILLHSIQAVSFELF
                     CIYRNPEMKFNRLISNKL"
     gene            4917..5909
                     /locus_tag="PKM34_03375"
     CDS             4917..5909
                     /locus_tag="PKM34_03375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ82657.1"
                     /translation="MKYFITGISGFIGSCLARELTSNGHSVNALVRNPEKVSPADHPG
                     VRFFKGDLFDTPVLLQAMEECDAVFHLAAFAKPWSKDPAYPHRVNVGGAVNVFEAALK
                     AGVKKVVFTSSAATMSPSDGDLPVDEDTQRTIPYFNAYETTKAEAESKAREYCGRGLP
                     VVMVNPSRVYGPGPLNPSNSTTKMMIGYKSGKWRIIPGDGSKIGNYVFIGDVVRGHIL
                     AAEKGREGERYILGGENVTFEDFFGILAEVSGRRRKMIHLPVPVMACIARAMEWQARF
                     TGIAPLITEDFVLKYLNDWCLSSAKAERELDYHITPLAEGVRQTLEWDFMKKAD"
     gene            complement(5906..>7174)
                     /locus_tag="PKM34_03380"
     CDS             complement(5906..>7174)
                     /locus_tag="PKM34_03380"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNQ82658.1"
                     /translation="TQSIALTRRLIEEGTEVIYEAGFAVDDLHCFIDILVRDGDKWKA
                     YEVKSSTQVKPVNLLDAAFQYYVITKSGLHVSDISLVVLDTGYEREGDLDIRSLFKTI
                     SVLEPVLKWQDKIEQSVEDFFFMLKCPVVPETGIGPHCSDPYDCDFKGHCWEHIPDYS
                     IFNISRLSADKKWELYRQGIVKFEDIPSDFRLNVAQWQQVIAELKGETHIDKKAIGRF
                     VSGLSYPLHFLDFESFQPAVPLYDHSHPYQQIVFQYSLHILDSPDALLQHRYFLAPAD
                     KADPRIPFISQLVEDINPTGDILVFNRSFEASRLNEISATFPNFQFQISNLTSRIKDL
                     MDIFQKRYYYVPEMKGSYSIKQVLPALAPQFSYEGLAIADGGSASHAFMALHSEPNPE
                     KIAETRNNLLEYCKLDTLAMVEILNVLQRI"
BASE COUNT         1823 a         1764 c         1736 g         1851 t
ORIGIN      
        1 tcgtggagag ttccttgatc ttgtaaagat tgacgactgt atcgttgtgg acatacatgt
       61 cggcgatgtc gagggcccgg tcacaaatcc tgaactccat gtgcaggtgg ttccccctgg
      121 cgcgtcccgt agcgcctccg agcccaatgg tatctcccgg ttccacccat tgatcggtct
      181 ttacccgtat tttcgaaaga tgggcataat agacctccat cccgctgtaa tgccgcacga
      241 tgaccaggtt gccgtatccg ctgcaatacc ttgcataacg caccttcccg ttaagcccgg
      301 ctaataccgg tgaaccgtaa tcaagcccga tatcccaccc ggcatgatag tatgtgcatc
      361 cccgccaaag cttcccgata gtcggcctga cgaatggatg tgccgtatcg cacagctcca
      421 ggcctgttcc gatcagggcg gtatcggtcg tcttccagcc gcatttgatg gaattggtca
      481 cccagtaatc gtcgtaaaca gtgatgatct tttttgcagc actgtcggat aagtggttga
      541 taagaagtgt gctgtcggct aatgccaagg aatccgccgg ttgcatcata accgtatcct
      601 gcgaaaaacc tgcaagggta aaaaaagtgg cgaaaacaga caaaatgaaa atcttccgca
      661 tcgctcgcct aattggaaat tcactcatcg ctttgtaccc cgggataact tggataattt
      721 aacattttcg ggccaaaagt aacagttttt gttgaaaatt tctatcaacg tgtctgtggt
      781 ttgatctgaa agatccgtgt tcattcgtat aaattcgcgt cattcgtgtg ctattaatag
      841 aacacgaata gacgcgaata caaagaattt acacgaatta atgttaaaat tacagagggc
      901 cccagagttt tttctgttca ctgctcaccc ttcactgctc acgcctcacg tttcaccggc
      961 acgtagatat ccgtcttcag gttctccggc cgggtgcgat cgggatcgtt caggtagaac
     1021 tccagcggcg gttcgtcacg aagttcatat ccgctttccg gcaaccagtt caaatagatc
     1081 tggtcgtaag ttttcgaaaa attctcatac ggaccggtat ggcggaaaat cgcatacaat
     1141 cctccttcga ggactttata cccgacctcc ccttccggcc tgatctcttt cttaactgta
     1201 acacaggcat catacctgag cttctccgtc tctgtgactg aaggatcatc gtacgaaaca
     1261 ccgatgaaat ccatcccgaa cgaaaacaac ttcttttcct tcatgaactt cgccagcttt
     1321 tcccatgccg ggccgacctt gttgtaatcc ccgatggcat ggacatatac caccggaata
     1381 ggcttgatct ctttgatctt aggtttcaaa ttcatcgctt taaaattaaa tgtggataac
     1441 tctacttttt ctaatcgcag ggatccctta ttttgacgat agtcgctggg cgacgtaccg
     1501 aaacgtttct gaaaagcttt ggtaaaggcg gccggtgtat cataaccgat ccggtaggcg
     1561 atatccgaaa ccggctccct ggaatatatc aacagggaag cggctgtgtc gagcctgacc
     1621 cttatgatat acgagccgag ggattcgcgc agatacgccc gcatgatctt gtgaaaatga
     1681 aacggcgaga agtgagccat cgacgccagc atctccagat ccatcttttc cccaaggttc
     1741 cggtcgatgt aaacaagcac cttgttaatt cgctcaaaat aaatggtctt ggtagttatc
     1801 tgtagcatcg cgctattgtt aagggttacc ggttaaaaaa tcacaagttc acaatatcaa
     1861 aaaatcaaaa gttcaaaagc tcacaagctc acaatatcaa aaaatcaaaa gctcacaagc
     1921 tcacaatacc aaaaaatcac aagttcaaaa tatcacaata tcacaaattc taaatatcca
     1981 caaatctact tcccgtatta taactccgct tttccattct tgcgatatta acataagatt
     2041 tgctttaaca gcaaaaatgc ccattatttc cgggaaattt tgcaactcat caaaaattga
     2101 tttgcaaaaa atagaccttt taagatttcc cctgtcttta gaattagaat tccataaaaa
     2161 caccttcccg gggaagattg atttttacca actgcaaatt ttcaggctta ccatggcata
     2221 aagccttttc gttaaggcat ttgcaggata taattttgga tcaaaaaaca agagctatga
     2281 aaacactatc atttaatgac aaagcaaagc accttctgat cctgatcacc ttattagtga
     2341 tttgcctgaa gatgatggcc gtaacaccgg aagaagaatt ccggttaatg aagcagaaaa
     2401 acgggataga cctgttttac cgctggatgg tcatgcccga aggcaaccgg gttaggcaga
     2461 tgaaggcggt ctttgaagga ggctggacaa aggaggatat catgtacctt cttaaaaacg
     2521 aacagcaggc catggcctgg atcccatctg cccgggagta ccgcaacctg aaagaggcga
     2581 cggctggtga atggttcagc tatatccgct tcagcgcgcc ctggcccctg gccgaccagg
     2641 attgcattct tcagtattct ttatcaacag aaccgtcggg tgaatcgacg atccggttta
     2701 cctgccggcc cgattacatt cccgaggtgg atgggattac ccggatgaag gacatcaacg
     2761 gttcgttcgt tatctgcccc ggaagcggcg aaaaagggag ggttgaatgt tacttcatct
     2821 cctccaaggc ttctgtcatc ccgcgctgga tcacagatcc tattataacc ggaagcatca
     2881 taagcctgat ggaaggcatg cgcggtcaat tatccggagt tgaatattct caaaccctgc
     2941 aaaaatgaaa aagctcacct tcaatcccgt tactctggcc gtcaacaagc tcaaaagcat
     3001 caaatcggat ccggaaaagg tctgttacgg gtttgctttc ggcaccttca tcagcgccac
     3061 gccgttcatc ggtttcaaat ggctggtggc actgcccatc gtgtggctgg ccaaatggaa
     3121 caagaccgcc tgcatgatcg gtatccttca ggtcaactat cttaccgggc cgttcttcta
     3181 tgccctggca tactttgtcg gtaaatcggt ctgtggttat gagaatacgt ttgaattacc
     3241 cgaccggatg acattcggag cggtaaagga tatctttttc ggtaatgcgg atgtgtttgt
     3301 atccctgctt gccggcggat tgcttctcag catccccatg actctcgggg ctttttacct
     3361 ggcccggtcg atattttaca ggaaattaaa tactgttatg tcatgaaaac acaggagacc
     3421 tataccctga ttaccggcgc cagtatgggg ctggggagag aactggcgac cgaatgtgcc
     3481 cgtaggggcc agaatctgct gctggtcgcg ctgcctggca gcggcttatc ggaattgcgc
     3541 gacgagctga aagaggactt tcggcaccat gtcgaatgtt acgaatgtga tctgagccat
     3601 gaagcggagc tggaaaagct ggcggcctgg gtgacagaga actattcggt cgaccggctg
     3661 atcaataacg cggggatcgg gggcactctc cggtttgaag aggccgatgc cggttatctc
     3721 gatacgatca tccatctgaa catccgggca accaccatcc tgagccggct tttattgcct
     3781 gaactgaaac gccatcccag cgcactgatc atgaatatct ccagcctggc cgcctttggc
     3841 ccgttgccat acaaaaccat ttatcctgcc tccaaggcat tcatctattc cttctccagg
     3901 ggcttgagca gggaattgat agataccggt gtaaaagtgg ttgtggtggc gcccgggcca
     3961 tttatctcca accccgatgt ggccaggcgg atcatgaagc agagctttat cgcccggctg
     4021 ggcgtcctct ccgcccggga aattacgctg aaggcgctga aaggcgccga gaaaggtcag
     4081 aacgtgatca tccccggtat ctggaacagg ttaaaccggc tgctgatgca actggtgcct
     4141 gaagacatcc gcctgagcgt tttgagcaat gtactgaaaa gagaactgcc gcttaaataa
     4201 acatttgcca tggaaccgga tactatcaaa gccgtttatg caattctggc caccgcagcc
     4261 gtttcagtgg cttattactt cgtgctttac cggatcggta aaatgaagaa accgggaacc
     4321 tgcagcgtgg ccattggttg ttacctgcta aggaaaatca ccggtttcct ggtcctcggc
     4381 gttgtgcctg tagccgttgc ctggttcgtt ttcggtcaga gtttggctga atcaggtttc
     4441 agggccggga caacggttac ctggctgcct tatacagctc tacttgcact ttttctgact
     4501 gccctgaatt acttcaactc atccaacatc cggttgcaaa aggcctatcc tgagctccgc
     4561 ctcgaaaact ggggtccggg ctccgccctg gtcctcgcct tcgggtggat cctttacctg
     4621 gtgggatatg agatgctgtt caggggtttg cttctgcata cctgcctgga agcattcggt
     4681 ttgtggccgg ccgtgatcat caacctgtcg ttgtacttta tccttcatct gcctaaaggg
     4741 ctgaaagagt cggtggcaac gatccccttc ggggctgtcg tcattttcct gaccgttaaa
     4801 tccgggtctg ttttaccggc tattctcctt cattccatac aggccgtttc atttgaatta
     4861 ttctgtattt accgtaatcc cgaaatgaaa ttcaaccgat taatatctaa taagttatga
     4921 aatattttat taccggtata tccggtttca taggatcctg ccttgccagg gaattgactt
     4981 cgaacgggca ttccgtcaat gcacttgtcc ggaatcctga aaaagtatcc cctgctgatc
     5041 atccgggcgt caggtttttt aagggcgacc tgtttgatac ccctgttctg ttgcaggcca
     5101 tggaggaatg cgatgcagtc tttcacctgg ctgccttcgc caaaccctgg tcgaaagatc
     5161 cggcttatcc gcatcgcgtg aatgtcggag gagccgttaa tgtttttgaa gctgcgctaa
     5221 aagccggtgt caaaaaggtt gtgttcacct cttcagccgc aacaatgtct ccatccgacg
     5281 gcgaccttcc cgttgatgaa gatactcaac ggacaatccc gtacttcaat gcgtatgaaa
     5341 cgacaaaagc agaagcagaa tcaaaagcaa gggaatactg tggcaggggt ttgcccgtcg
     5401 tcatggtgaa tccatcaagg gtatacggcc cgggaccctt aaatccgagc aattcaacca
     5461 ctaaaatgat gattggatat aagtcgggga aatggaggat aattccgggt gatggttcga
     5521 aaatcgggaa ttatgttttt atcggtgacg tggtccgcgg ccatattctt gccgctgaaa
     5581 aagggcgtga aggtgaacgg tatatcctgg gcggtgagaa tgttaccttc gaagactttt
     5641 tcggaatcct ggcagaagtt tccggaagaa ggcgtaaaat gatccatcta cccgtacccg
     5701 ttatggcatg tatagccagg gccatggaat ggcaggcccg gtttaccggc atcgctccgc
     5761 tgatcaccga agattttgtt cttaaatatc tcaacgattg gtgcttgagc agcgcaaaag
     5821 cagaacggga actggattac catataacac ctctggctga gggagtcagg cagaccctgg
     5881 aatgggattt tatgaaaaaa gctgattaga tcctctgtaa aacattcagg atctccacca
     5941 tggccagggt atccagttta cagtattcaa gcaggttatt ccgggtttct gcgattttct
     6001 cagggttggg ttcggaatgc agggccataa aggcgtggct ggccgagcct ccgtcggcaa
     6061 tggccaagcc ttcataactg aattgcggcg ccagtgcggg aagcacctgc ttaatggaat
     6121 aagaaccttt catttcgggg acgtaataat accgtttctg gaagatgtcc atcaggtctt
     6181 taattcggga ggtcagattc gaaatttgaa actggaaatt cgggaacgtg gcggagattt
     6241 cgttcagcct ggaggcttcg aaggagcggt tgaaaacaag gatatcgccg gtagggttta
     6301 tatcttctac caattgggag ataaatggga ttcgcgggtc cgctttatcg gccggggcca
     6361 ggaaatagcg gtgttgcagc agggcatccg gtgaatccaa aatatgcagc gaatactgaa
     6421 agacgatttg ctggtaaggg tggctgtgat cgtacaacgg caccgctggc tggaacgatt
     6481 cgaaatcgag gaaatggagc ggataactga ggccggagac aaagcggccg atcgcttttt
     6541 tgtcgatatg cgtctcccct ttcaactccg cgatgacctg ttgccactgg gctacattca
     6601 gcctgaaatc cgaaggaata tcttcaaact tcacgatccc ctggcggtaa agctcccatt
     6661 tcttatcggc gctaagccgg gaaatattga agatggaata gtcgggaata tgctcccagc
     6721 aatgcccttt gaaatcacag tcgtacggat cgctgcagtg aggaccgatg ccggtttccg
     6781 gtaccacggg acatttcagc atgaagaaga agtcttcaac ggattgttca atcttatcct
     6841 gccatttcag caccggttcc agtacactta tggttttgaa gagagaacgg atatcaagat
     6901 caccctctct ttcgtagcct gtatccagca caaccagtga aatatcggaa acatgcaacc
     6961 cgctttttgt gatgacataa tactggaaag cggcgtccag caggttcacc ggctttacct
     7021 gtgtggagct cttgacttcg tatgctttcc atttatctcc atccctgaca aggatatcga
     7081 taaagcaatg caggtcatca acagcaaaac cggcttcata gataacctcc gtgccctctt
     7141 cgatcagtct gcgcgtcaat gcaatgcttt gggt
//
LOCUS       DAOQXS010000075         7123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10909_length_7123_cov_3.318195, whole genome shotgun sequence.
ACCESSION   DAOQXS010000075 DAOQXS010000000
VERSION     DAOQXS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10909_length_7123_cov_3.318195"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2252
                     /locus_tag="PKM34_03385"
     CDS             171..2252
                     /locus_tag="PKM34_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ82659.1"
                     /translation="MRRSEITIEQASDEGTGLLTLADLLNKRAACTPDRCAFIFLRDG
                     ENDEERITYGQLYNSALAISRELTEATRPGDRALMFYPAGLEFIKALFGCFYAGVIAV
                     PAYPPRKNRSLERLRALASDSGASIVLSTDDIRQTSQRSFSDTIELQKLTWLSTHPIS
                     KSQIQSPNLQISKSSNLQISKSPNPQIALLQYTSGSTGKPKGVMVTHHNLMRNLEFLR
                     QAVELTPDSISVHWLPVFHDMGLVFGVLESIYTGYTGILMPPVSFIQKPSRWLKAISR
                     YKATLAGAPNFAYDLCVADISEDECKDLDLSSLKSLYNGAEPVRASTMKAFTGKFGPF
                     GFREEMFYPTYGMAEATLILSGGKVCDKPVVVYVDKTELEKNRIRIVAEENENAYSLV
                     SVGNPWIDTSITIVNPDTLEPCPGDVVGELWVSGSIITKGYWENDEETRNIFNAYTAS
                     NGRGPFLRTGDLGFFHEGKLFISGRLKDLIIIRGSNYYPQDIEYLTESSHKAIRANSS
                     AAFSVIIDDTERLIIIAEVERTAMKDLNVEEVVNAIRTKIAAEMELAVYGIQLIRTAT
                     IMKTSSGKIQRKACQKAFLSHSLEVVGESFLTDYKEDNEESLSPPDLVSIRAWLMTWI
                     HLKLKIRLEKIDLSKPITVYGLNSMKAVQLQQDFLENFQVNFPPYLFFERISLNELSE
                     RAFRLIKESEK"
     gene            complement(2255..2491)
                     /locus_tag="PKM34_03390"
     CDS             complement(2255..2491)
                     /locus_tag="PKM34_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ82660.1"
                     /translation="MTIEDFIARIEEEFDDLEPGKLRPESNFREVFEWNSINALILIA
                     LVKTNYDVAINAEDITKSKTVNDLYKIVKERIAS"
     gene            complement(3035..4741)
                     /locus_tag="PKM34_03395"
     CDS             complement(3035..4741)
                     /locus_tag="PKM34_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ82661.1"
                     /translation="MIASIRNRKTLADLLISAAGESPENGVGFIRADGSIQFLTYPGL
                     LAEAETIASGIRSEGIKEGESVMIIITRNEVLVPTLWGCILAGVVPTILQPPVSFTEV
                     NPPASKIENVYSILDKPGIILSDDLAGSFNSTVISPESIIPASDLAAGKNPVRQELSN
                     ETENLAFIQFSSGSTGDPKGIMLTHRNILVNIAAICVGLDIHKSDVMCNWMPLYHDMG
                     LFGFHICPVFAQSNHYLIDPVEFIKKPTLWLDVIEHSGSTITGCPNFGQALLLRYLKN
                     RQESGRNLSSLKAIVNGAEPISNRIMSEFTGKLSVSGLRKEVMMPAYGMAENTLAITF
                     ADLWSEPLVMNFNRTLLQAQGIAVREEKKSGETVELVSVGKALNDVEIKIADDDGNGL
                     AEGMEGHIRIRGKSVTSGYINNPEATAEAFEDGWLKTGDKGFFYDGQLYITGRIKDII
                     FVHGQNLYAHDLENLASKHADIPYGKVIIGGSFDPKKGKDQVILFLVGSANQALCDLF
                     LELKGFFRDTYGVHIDVFVPVRSNQVPKTSSGKIQRYKLLEDYRDGVFDEAIAEIRRL
                     MR"
     gene            4850..6325
                     /locus_tag="PKM34_03400"
     CDS             4850..6325
                     /locus_tag="PKM34_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ82662.1"
                     /translation="MRKKLLLINPANELRQGFTNDDSTRFMPIGLGIVAALTPKHWEV
                     ELIDESYERFSFRFADLVAFTGFTANAPRAYKIASVYREKGIHTVMGGIHASLATDEV
                     AEYVDTVFTGEAEGAWKDLITDFEAGKIKKLYDGGNVDIHSVPHVRRDIYTYPYVYDL
                     VSTSRGCPLGCEFCSVTQLCGKTYRERKVEDILDELEETTRPLIFFVDDNFIQNTKRS
                     HERAISLFRGMVERKLNKFWVCQATVNFAENEEVLHWARKSGCMMVFVGVEAENEEAL
                     KDVRKNLNLKRGVQSYEQTFRKAHKHGIGILAFMIFGMESDKKEDLFARRDYILRSSL
                     DAYQCAIMTPLPGTVLFERVKARNQIVLNNYPDDWQQYDCHAATIDTASMDHADIDRS
                     MYEIWLSLYNKKTIRKKMFRTLWNTRSFRTAYWAYAANHNYGRMFLERFIQTDKECLN
                     SRLEWVGKKRSMYLKLTDALLWVFYQTAWKKILKRVSGKKA"
     gene            6330..>7123
                     /locus_tag="PKM34_03405"
     CDS             6330..>7123
                     /locus_tag="PKM34_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNQ82663.1"
                     /translation="MDAERRRKLILVNPVNRVRIGFMNSEGTIAMPLGLGIVAALTPE
                     HWEVELVDEYFGEFNLKSADLVALTGFTPAAYRAYEIAAMCRQAGIHTVMGGIHASMC
                     PEEAARFVDTVFLGEAEGAWPALIADFEAGRIKSRYDGGIVAVNDIASPRRDIFDRYP
                     YVYDLLQTSRGCPMGCEFCSVTQMCGKTYREREINAVIADLEKTRRPLLFFVDDNLVN
                     NKKGSEERAINLFKAMVDRGLTREWYSQAAVNFAANDEVLYWARKSG"
BASE COUNT         1836 a         1738 c         1710 g         1839 t
ORIGIN      
        1 gcagtggccc tgcagcagga cttcctggaa agatatagcg tcagttttcc cccttatctg
       61 ttttttgaaa aatcttcatt aagagaactt tgtgagaaag ccataaaaat tattaaagaa
      121 aactagaaga actataaaag tttttatagt atatttgttt tctttacctc atgagacgct
      181 ctgaaattac tattgaacag gcttcagatg aaggcacagg cctgttaacg ctcgctgacc
      241 tattgaataa gagggctgcc tgcacacctg accggtgtgc gtttatattt ttacgcgacg
      301 gggagaacga tgaagaaagg atcacatacg gacagcttta taattcagcg ttagccattt
      361 cccgggaatt aactgaagcc acccggcccg gcgaccgcgc ccttatgttc tatcctgccg
      421 ggcttgaatt tatcaaagct ctctttggtt gtttttacgc aggagtcatt gcagtcccgg
      481 cataccctcc caggaaaaac cgttcgcttg agcggctcag ggccctggca agcgattccg
      541 gcgccagcat tgtgctttca acggacgaca tcaggcaaac cagccaacga tcattttcag
      601 acaccatcga acttcaaaaa ttgacctggc tctcaaccca tcccatctca aaatcacaaa
      661 tccaatcccc caatctccaa atctccaaat cctccaatct ccaaatctcc aaatctccaa
      721 atccccaaat cgccctgctc cagtacacct ccggctctac aggtaaaccc aaaggcgtta
      781 tggtgaccca tcataacctg atgaggaacc ttgaatttct gagacaagct gttgagctta
      841 caccggattc tatttccgta cactggcttc cggtttttca cgatatgggc ctggtttttg
      901 gtgtcctgga atccatttac acaggatata ctggtattct tatgccgccg gtttcattca
      961 tccagaaacc gtcgagatgg ctgaaagcca tatccaggta taaagcaaca ctggccggcg
     1021 cgcctaattt cgcatacgat ctttgtgttg cggatatttc ggaagacgaa tgcaaagacc
     1081 tggacctgag tagtctaaaa tcactgtata acggggctga accggtaaga gcctccacca
     1141 tgaaagcatt taccggaaaa ttcggtccgt ttggattccg tgaggagatg ttttatccga
     1201 cgtatggaat ggcagaggca acgctcatac tctcgggagg caaggtgtgc gataaacccg
     1261 ttgtagttta tgtagataag accgaacttg aaaaaaaccg gatccgtatt gttgccgaag
     1321 aaaatgaaaa tgcctatagc ctcgtttccg taggaaatcc ctggattgat acttccatca
     1381 ccattgttaa tcctgacaca ttggaaccct gccccggcga cgtagtgggt gagctttggg
     1441 tatcgggctc gattatcaca aaaggatatt gggaaaatga tgaggaaacc cggaacattt
     1501 ttaatgcata tacagccagc aacggcagag ggccatttct cagaaccggc gaccttggct
     1561 tttttcacga aggaaagctc tttatctccg gcagattaaa agatttaatc ataatccggg
     1621 ggagtaatta ttaccctcag gatattgaat atcttaccga aagcagccat aaagctatca
     1681 gagcaaattc cagcgcggca ttttcggtta taattgatga tacagaaaga ttgataatta
     1741 tcgctgaggt ggaacgcaca gcgatgaagg atctgaacgt ggaggaggtt gtcaatgcca
     1801 tccggacaaa aattgccgct gaaatggaac tggccgtcta tggtattcag cttatccgta
     1861 cagctaccat tatgaaaaca tcaagtggaa aaattcagcg aaaagcttgt cagaaggctt
     1921 tcttaagcca ctccctggaa gttgtcggag agtcctttct gactgattat aaagaagata
     1981 atgaggaatc tctatcacca cccgacctgg tgtctatccg tgcatggctg atgacatgga
     2041 tccatctcaa attaaagatc aggctggaga agattgattt atccaaacct ataaccgttt
     2101 acgggctgaa ttccatgaaa gccgtacaac tccagcagga tttcctggag aatttccagg
     2161 tgaactttcc tccttacctg ttttttgaaa gaatatcgct gaatgaactg tcagaaagag
     2221 ctttccggtt gataaaggaa tctgaaaaat aactttatga agctattcgt tcctttacga
     2281 tcttatacag atcattcacc gttttggatt tggtgatgtc ttcggcattg atggccacat
     2341 cgtaattggt ttttaccagg gcgataagga tcaatgcgtt gatcgagttc cactcaaaca
     2401 cctccctgaa attcgattcc ggcctgagct tgccgggttc caggtcgtca aactcctctt
     2461 caatccgggc aataaaatct tcgatggtca tttctaataa atttgaagcg gcaaaggtat
     2521 tttatttctc gcagatttca tgatcaaatt gatatgaatg acaatttcac tgaaacagtc
     2581 gactaacatt tggataccgg cttaagcctg aatgacggcg aatgtatgcc agggtaaaaa
     2641 gcgatgaggc aattgtcatc ccgagtttaa agactttaac aaactccaca atatgggatg
     2701 atattagctt ttcattactc cggaggagtt ccacatttgt agcaaaggcg atggcatccg
     2761 cacaattttc ccctcccgcg ataatccggt ttttcaagct aatatcttca tgggagggga
     2821 ttaaggggag gggaaagatg cattttcggt ttccctcccc aacccctccc tgtgaaggtt
     2881 tttcgctata atccaatatt tccaaaggga ggggcttttg ttctttcccg tcctttttgc
     2941 tacaaatagg gctctcctcc ggagagcggt tcagtagaaa tttactaact tgtcattggt
     3001 agcgaagcga aggaccaaaa tcgtgctgag gcccttacct catcaacctc ctgatttctg
     3061 cgatcgcctc atcaaaaacc ccgtcgcggt aatcttccag cagcttatac ctttgaattt
     3121 tcccgctgct tgttttcggc acctggttcg accgcaccgg cacaaataca tcaatatgta
     3181 ctccgtaggt gtccctgaaa aatcctttca gttccaggaa cagatcacaa agggcctggt
     3241 ttgccgatcc gaccaggaac aggatcacct ggtcttttcc ttttttcgga tcgaaagagc
     3301 cgccgatgat gactttaccg taggggatgt cggcatgttt cgaggccagg ttctccaggt
     3361 catgggcata caggttctgg ccgtgcacaa aaatgatatc cttgatcctg ccggtgatat
     3421 agagctgtcc gtcatagaaa aatcccttgt cgcctgtttt cagccatccg tcttcgaacg
     3481 cttcggccgt ggcctccgga ttattgatat agcccgatgt tacgcttttg ccccgaatcc
     3541 ggatgtgccc ttccatcccc tctgccagcc catttccatc atcatccgct atttttattt
     3601 ccacatcatt cagcgcttta ccgacactca ccagttcgac tgtttcccct gatttttttt
     3661 cctccctcac ggctatcccc tgagcctgga gcaaagtccg gttgaaattc atgacgagcg
     3721 gttcgctcca caggtcggca aaggtgatcg ccagcgtgtt ctcagccatg ccataagccg
     3781 gcatcatgac ctctttacgt aacccggata ccgaaagttt gcctgtaaat tccgacatga
     3841 tccggttgga gatgggttcc gcaccgttca ctattgcttt taatgaagaa aggttccgtc
     3901 cgctttcctg cctgtttttc aaatacctga gcaacaaggc ctggccgaaa ttcggacaac
     3961 cggtgatcgt gctccccgaa tgttcaataa cgtcaagcca taaggtaggt tttttgatga
     4021 attcgaccgg atcgatcagg tagtggttgc tctgggcaaa taccgggcat atgtgaaatc
     4081 cgaacaatcc catgtcgtgg tatagcggca tccagttgca catgacgtcg cttttgtgaa
     4141 tatcgagccc gacacaaatg gccgcaatat ttacaaggat attccggtga gtcagcatga
     4201 tccctttggg atcgccggtg cttcccgaag aaaactggat aaatgccagg ttctccgttt
     4261 cgtttgataa ttcctgtctg acggggtttt tgccggcagc cagatcgctt gccgggataa
     4321 tgctttcggg agagatgacg gttgaattga aactcccggc caggtcgtcc gacaggatga
     4381 tcccgggctt gtccaggatg ctgtaaacgt tttcgatttt cgaggcgggc ggattgactt
     4441 cggtgaatga aaccggcggc tggaggatcg tcggaacaac gccggccagg atgcaccccc
     4501 agagcgtcgg taccaaaacc tcgttccgtg tgatgatgat catgacagat tcaccttctt
     4561 ttatcccttc cgaccggatt ccggaggcga ttgtttccgc ttccgccagc aacccggggt
     4621 atgtcaggaa ttgaatgctg ccgtcggccc ggataaatcc gaccccgttt tccggcgatt
     4681 caccggctgc acttatcagc aggtcggcca gtgttttcct gtttcggatg ctcgcgatca
     4741 tatcctggag atcataaggc gcaaaagtaa caattaatgt aaaaagggag ggttggagtc
     4801 cctttttccc tatttttgga tttggttatt cacgatctta catctgaaag tgcgcaagaa
     4861 acttctcctg ataaacccgg caaacgagtt aagacagggt ttcacaaacg acgacagtac
     4921 ccgttttatg ccgatcggcc tgggcatcgt agccgccctt acccccaaac actgggaagt
     4981 ggaactgatc gatgagagtt atgaacgatt ttcattcaga ttcgctgacc tggttgcttt
     5041 taccggtttt accgccaatg cgccgcgcgc ctataaaata gcttctgtct accgggaaaa
     5101 gggaatccat accgttatgg gtggcatcca tgcctccctg gctacggatg aagtcgctga
     5161 atatgttgat accgttttta ccggcgaagc ggaaggcgca tggaaggatc tgatcaccga
     5221 ttttgaggca ggtaaaataa aaaaactgta tgatggcgga aatgtcgata tccattctgt
     5281 tcctcatgtc aggagggata tctacaccta tccttatgtt tacgacctgg tcagcacttc
     5341 ccgcggatgc ccgctgggat gcgaattctg ttccgtaacc cagttgtgcg ggaaaaccta
     5401 ccgcgagcgt aaagtggagg acatcctgga cgaactggag gaaacaaccc gtccccttat
     5461 tttttttgtg gatgataatt ttatccagaa tacaaaacga tcgcacgaac gcgccatcag
     5521 cctcttccgg ggaatggtcg aacggaaact gaataaattc tgggtgtgcc aggctactgt
     5581 aaatttcgct gaaaatgagg aagttctcca ttgggccagg aaaagcggct gtatgatggt
     5641 gtttgtcggt gtggaagcgg aaaatgaaga ggccctgaaa gatgtcagaa aaaacctgaa
     5701 tctgaaaagg ggggttcaat cctatgaaca gaccttccgg aaagcccaca aacacgggat
     5761 cgggatcctg gcttttatga tcttcggcat ggagagcgat aaaaaggaag atttattcgc
     5821 ccggagggat tacatcctga gaagctcact ggatgcttac caatgcgcga tcatgacccc
     5881 tttgcccgga acggtactgt tcgaacgcgt gaaagcccgc aaccagatcg tgctgaacaa
     5941 ttatcctgac gactggcaac agtacgattg ccatgctgct accatcgata cagcttctat
     6001 ggatcatgct gatattgaca ggagtatgta cgagatatgg ctttcgctct ataacaaaaa
     6061 aaccatccgt aagaaaatgt tcaggaccct ctggaatacg cgaagtttcc gaaccgctta
     6121 ctgggcgtat gccgcaaacc ataattatgg ccggatgttc ctggaaaggt ttatacaaac
     6181 cgataaggaa tgcttgaact cccggctgga atgggttggg aagaagcgtt caatgtatct
     6241 gaagttgacc gatgccctcc tttgggtctt ctatcaaacc gcctggaaaa aaatcctgaa
     6301 aagagtctcc ggtaaaaaag catgaattga tggatgcaga acgtcgcaga aagctgatat
     6361 tggtcaatcc ggtcaaccgg gtcaggatcg gctttatgaa ttcggaaggg actattgcca
     6421 tgcccctcgg cctggggatc gtggcggccc tgaccccgga acactgggag gtggagctgg
     6481 tcgatgagta tttcggtgag ttcaacctca aatctgccga tcttgtagcc ttgacgggat
     6541 ttacgccggc tgcgtatcgt gcctatgaga tcgccgctat gtgccggcag gccgggatcc
     6601 atactgttat gggagggatt catgcctcga tgtgccccga agaagcggcc cggtttgtcg
     6661 ataccgtctt cctcggcgaa gcggaaggcg cctggcccgc cctgatcgcc gattttgaag
     6721 ccggccgcat taaaagccgg tacgacggag ggattgttgc ggtgaacgac attgcctccc
     6781 ccaggaggga tatcttcgac cgttatccct atgtgtacga cctactccag acttcacgcg
     6841 gctgcccgat gggatgcgag ttctgctccg tgacacagat gtgcggcaaa acctaccggg
     6901 aacgcgaaat caacgcggtg atcgccgacc tggaaaaaac ccggcgaccg ctgctttttt
     6961 tcgtcgacga taacctggtc aataataaaa aagggtctga agaaagggca attaaccttt
     7021 tcaaagccat ggtcgaccgg ggactcaccc gggaatggta ttcacaggcg gcagtgaatt
     7081 tcgccgccaa cgacgaagtt ctttactggg cccgtaaaag cgg
//
LOCUS       DAOQXS010000076         7109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10937_length_7109_cov_3.370712, whole genome shotgun sequence.
ACCESSION   DAOQXS010000076 DAOQXS010000000
VERSION     DAOQXS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10937_length_7109_cov_3.370712"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKM34_03410"
     CDS             <1..791
                     /locus_tag="PKM34_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ82664.1"
                     /translation="SSSRPAIGCHFALDVSNSMLSQDIRPDRLTRAVQGISKLIDRLE
                     GDRIGIVVFAGKAYVQLPITTDYSAAKMFLSTISTQMVPSQGTAIGEAIDLSLKSFKT
                     DERSKAIIIITDGENHEGNAVEAAQAAAENGIKIFTIGMGSPEGSPIPLFDKFGRQTG
                     YKADRDGRTIISRLDETTLQQVASAGNGIYVRASTGQDGLSKILDEINALEKQEIETR
                     MFSEYEGRFQYFLAIALFFLILEFFIPDKKSLWTDKIKLFNRLS"
     gene            794..1561
                     /locus_tag="PKM34_03415"
     CDS             794..1561
                     /locus_tag="PKM34_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82665.1"
                     /translation="MKRHLHILLLLLLPAPLLVAQQENRFIRQGNKQYDDEKFKEAEV
                     SYIKSTQAKNASHKGFYNLGNAWYMQENYLQASAAFDTLRTFRMDDDTRAKSYYNLGN
                     AMLKSAQDSAQLAGKFLPASVEAYKQSLRLNPEDEEAKYNLAYAQKLMQQQQQQQQQQ
                     QQNQDQKDQQKEQQKDQQKQQDQKDQQQDQQQQDQQNAEQANQDQQQANAEPQQISKE
                     DAERILEALKNDEKETLEKLSEAKIQSVRVVKTDKDW"
     gene            1578..3431
                     /locus_tag="PKM34_03420"
     CDS             1578..3431
                     /locus_tag="PKM34_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNQ82666.1"
                     /translation="MNRILKIMFVLALMIPVFGILNGQDIEFSAATKNNVQVGEQFRA
                     VYTVNSKVDRFNGPDFEGFNVLSGPNSSQNQSYQFINGKVSQSFQITYTYYLQATREG
                     TFTIPAASISIGNQTFQSNPLTINVSQSASGQAPPAQGNVRQANRNTGTPGNNEIFIK
                     ASVDKAKPFQGEQVIITYKIYTAVPVSQINITKISSFPGFWSKNLLGDDEQLKQTTEY
                     INGKEYVVAELRKLALFAQRSGELKIEPMEMECLAQVIVESQRTRDPFFDSFFNDPFF
                     SRNYQNVEIKLESNPLTIDVTPLPASGKPLDFSGAVGSFTLSSEIDRNELKTNEALNL
                     KVTITGKGNLELIEALPLTFPPDFEVYDPRISNNLSKSTTGVSGSRVFEYLLIPRNPG
                     EFQIRPAGFSYFDPSRQSYATLTTPAYGIKVLKGDNEPSGVVYSGVSQKNIQFIGSDI
                     RHIKTGQIPLHPVNSFLISSTALLLWLVVPALLFTAGLIFWARYKKQQGDIALVRNRK
                     ATKVARKNLKKAGEYLEAQNTEAFFEEVSRAMWGYISNKFNIPLSDLSIESVSQRLAG
                     KKVSSENILMFTEVLENCEFARFAPGDKPGRMQEIYNSALNVISRIEQELK"
     gene            3439..4200
                     /locus_tag="PKM34_03425"
     CDS             3439..4200
                     /locus_tag="PKM34_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82667.1"
                     /translation="MKRSAITVLTILLALLAQAQPAAISSLVRNGNEAYTAGDYKGAV
                     EQYESALRSGFESAELYFNLGNAYFKADDIPSAVLFYEKALRLDPSDENIHFNLALAN
                     SRITDKIEPLPEFFLKSWWNGIRDTFSSNEWASAGIFTFLILLAAVAIFIVSGSVMVR
                     KVSFWTGVILLMFTLTSFLFSLNSYHEYSRQSSAIIFTPTVTVKSSPTDGSVDLFVIH
                     EGTKVFITDRVDDWSEVRLVNGNVGWIKTNTFRPI"
     gene            4466..>7109
                     /locus_tag="PKM34_03430"
     CDS             4466..>7109
                     /locus_tag="PKM34_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82668.1"
                     /translation="MNKRPLPFGYFILTMALSAFLISATLTGDEKIEARQETESDYQA
                     RIRNNQLTGQITPADYLKAQQQIRELAANRSGGAYDFDWQLLGPNNLGGRTRAILFDN
                     RDATGNTVYAGSVLGGLFKSDNQGSKWYKIDEANGGLYVSCITQAGNGDIYVGTGEGF
                     TVQQYTVFGEWRYSGGLMGQGIFKSTDGVNFTRLASTNPTINGNNELEWGFINELAAH
                     PSNGALYASTNTGLKYSSDGGNNWQAAKMADGTILNLSSGDVKMAANGLVVAEVSNKC
                     YVSENGNFDNFQLRSGKASFNLPDTSAIGRIEFAIAPANNDIIYALVVKTSGALYNVY
                     RSDDKGATWSVVGPGGSANFNVFNTGTASATGNGLFAATIEVYPDDPYHVLIGGYDLW
                     EGKKIQDDGFYQWVLRSTSSANWLSVAFLWQGHHVYRFAPGSSEKVLIGTNGGISLGL
                     LDPEFFQFQFMNKDYIASQFYTVSPTMEKKDVVGGAQDIGTIYIRGSVNPADSRRGED
                     IWTTQSNVPDGRTGGYCAWSTIYPTAVIYSRFPQPEGSPETFIRRNEFSGGPDWALNN
                     TFTTAYLSTSFLPPFVLYENFEDYNSKDSTEFKANRDLAAGTTVWVESNNGKRPFKHV
                     LEAAIDSSETVMVKDIISTRFFIGGDNRVMMTKQMIQFDVSPEWFTISDSLRGGVRGK
                     PQAMAYSSDANHLFVGTQKGKLYRISNIKYAYNNETADVSSPYCVISTKQIPVYLPGT
                     TTEITQVITSVAVDPNNDEKVIITLGNYGNETYVYYTENALDENPVFHSAQGNLPQMP
                     AYSSLIEMDAANHLVFVGTEHGIYVTNDIGSSNPTWTAENANIGAVPVFMLKQQTIRK
                     TNDTLVFVNIDTTYLVHYGTNNYGV"
BASE COUNT         1961 a         1793 c         1762 g         1593 t
ORIGIN      
        1 caagctcgtc caggcccgcg atcggatgcc acttcgccct cgacgtgtcg aacagcatgc
       61 tctcgcagga tatccgccct gatcgattaa cccgggccgt gcagggaatc tccaagctga
      121 tcgaccggct ggaaggcgac cggatcggga tcgtcgtttt cgccgggaaa gcctatgtcc
      181 agcttcccat caccaccgat tattcggctg caaaaatgtt cctgtcgacc atatccacgc
      241 aaatggtacc ctcacagggc actgccatcg gcgaagccat cgacctatcc ctgaaatcct
      301 tcaaaacgga tgaaaggagc aaagcgatca tcatcatcac cgacggggag aatcacgagg
      361 gaaatgcagt ggaggccgcg caggcagcgg ctgagaacgg gatcaagatc ttcaccatcg
      421 ggatgggatc acccgaaggg tcgcctatac ccttgttcga caaattcggc aggcaaacgg
      481 gctacaaagc cgaccgggat ggccgcacga tcatctcccg gcttgatgaa acaaccctgc
      541 agcaggtagc cagcgccggg aacgggatct atgtcagggc ctccaccggg caggacgggc
      601 tttccaaaat cctggacgaa attaatgccc ttgaaaaaca ggaaattgaa acccggatgt
      661 tctcggaata tgaagggaga ttccagtatt ttctggccat tgccttattc tttttgatcc
      721 tggagttttt tattccggat aaaaaaagct tatggacaga caaaatcaaa ctgtttaacc
      781 gtttatcata actatgaaac gccatctcca tatccttctc ctgttgctgc tgccggcgcc
      841 gttactggtg gcccagcagg aaaaccgctt tatccggcag gggaacaagc aatatgatga
      901 tgaaaagttt aaagaagccg aggtcagtta tataaaatcc acccaggcaa agaacgcttc
      961 gcacaaagga ttttacaatc tcggaaatgc ctggtatatg caggaaaatt acctccaggc
     1021 atccgctgca ttcgataccc tgcggacctt cagaatggat gatgataccc gggcaaaatc
     1081 atattataac ctgggaaatg ctatgctcaa gagcgcccag gacagtgcac agctggccgg
     1141 aaaattcctg ccagccagcg ttgaagctta caaacagtcc cttcgtctca acccggaaga
     1201 cgaagaagcc aaatataacc tggcttatgc ccagaaactg atgcagcaac agcagcaaca
     1261 gcagcagcaa caacagcaaa accaggatca gaaagatcag caaaaggagc agcagaaaga
     1321 tcagcagaaa cagcaggatc agaaagatca gcaacaagat cagcagcagc aggatcagca
     1381 gaatgcggaa caggccaacc aggatcagca gcaagccaac gcagagcccc agcagatttc
     1441 aaaggaggat gccgaaagga tcctggaagc gctgaaaaat gatgaaaaag agacgcttga
     1501 gaagttgtcg gaagctaaaa ttcaatcggt cagggtggta aagaccgata aagactggta
     1561 ggcaatacgg aattattatg aatagaatcc tgaaaataat gtttgttctc gccctgatga
     1621 tcccggtctt cggcattctc aacggccagg acattgagtt ctcggcggcc acgaagaaca
     1681 atgtccaggt cggggaacaa ttcagggctg tatatacggt gaacagcaag gttgaccggt
     1741 ttaacggccc cgattttgaa ggatttaatg tactgagcgg gccgaattcc tcgcaaaacc
     1801 agagctacca gttcatcaac ggcaaggtgt cgcagtcgtt ccagatcact tacacttatt
     1861 acctgcaggc tacccgggag gggacattca ccatcccggc ggcctcaatt tcgatcggca
     1921 atcagacctt ccagtccaat ccgctgacca tcaatgtcag ccagtcggct tcaggacaag
     1981 cacctccggc acagggcaat gtaaggcagg caaaccggaa cacaggcaca cccggaaaca
     2041 atgagatttt catcaaagcc agtgtcgata aagcaaaacc gttccaggga gaacaggtta
     2101 tcatcaccta caaaatttac acggctgtgc ccgtttcaca gatcaatatc acgaaaatct
     2161 cctctttccc gggtttctgg tcaaaaaatc tgttgggtga tgacgaacag ttaaaacaga
     2221 cgactgagta tatcaacggg aaggagtatg tcgtggccga attgcggaaa ctggcgcttt
     2281 ttgcccagcg cagcggcgag ttgaagatcg agccaatgga gatggagtgc ctggcccagg
     2341 tcatagtgga gagccaacgg acacgggatc ccttcttcga cagttttttc aatgatcctt
     2401 ttttcagcag gaattaccag aacgtggaaa taaagcttga atccaatccg ctgacgattg
     2461 atgtaacccc gctgccggct tccggtaagc ccctggattt cagcggagcg gtgggatcgt
     2521 tcaccctttc ctcggagatt gaccgtaacg aactgaaaac caatgaagcg ctgaatctga
     2581 aagtgaccat tacgggcaaa ggcaacctcg aactgatcga agcgcttcca ttgacttttc
     2641 ctcctgattt cgaggtctat gatcccagga tctccaataa tctcagcaaa agtaccacag
     2701 gggtttcggg cagcagggta ttcgaatacc tgcttattcc gaggaatccg ggtgaattcc
     2761 agatcaggcc ggccggattt tcctatttcg atccttccag gcaaagctat gcaacattaa
     2821 ccacaccggc ttacggcatc aaggtactga agggcgacaa tgaaccgtcg ggtgtggtat
     2881 acagcggagt cagccagaag aacatccagt tcatcgggtc cgacatacgc cacatcaaga
     2941 ccggacagat tccactgcac ccggtcaatt cctttttgat cagttcaacc gccttgttgc
     3001 tctggctggt ggtccccgcc cttcttttca cagccgggct cattttttgg gccaggtata
     3061 aaaagcaaca gggcgacatt gccctggttc gcaaccggaa agccacgaaa gttgccagga
     3121 aaaaccttaa aaaagcgggt gaatacctgg aggcacaaaa taccgaagct ttttttgaag
     3181 aagtgagcag ggcgatgtgg ggctatatca gcaacaaatt caacataccc ctgtcggacc
     3241 tctcaataga aagcgtgagc cagcgacttg ccggcaaaaa ggtcagcagc gaaaatatcc
     3301 tgatgttcac cgaagttctt gaaaactgtg aatttgcccg ttttgcgccg ggcgacaaac
     3361 cgggacggat gcaggagatt tacaactcag ccctgaacgt aatcagccgt attgaacagg
     3421 aactgaaata acaaagagat gaagagatca gcgataaccg ttttgacaat cctgctggct
     3481 ttgctggcac aggcgcaacc tgcggcgatc agctcactgg tacggaacgg taatgaagcc
     3541 tacacagccg gtgactacaa gggagccgtc gaacaatacg aaagcgcatt gcggtcaggg
     3601 tttgaatcgg ctgaattgta tttcaacctg gggaatgctt atttcaaggc ggacgatatc
     3661 ccgtctgctg ttctgtttta cgagaaagcg ctcaggctgg atccttcgga tgagaatatc
     3721 cattttaacc ttgcgctggc caacagccgt attacagata aaatagaacc gctacccgaa
     3781 tttttcctga aatcgtggtg gaacggcata cgcgatacct tttcatcgaa cgagtgggcc
     3841 agtgcgggca tattcacttt cctgatcctt ctggcggccg tcgccatttt catcgtttcg
     3901 ggttcggtca tggtccgaaa agtcagtttc tggactggcg tgattctcct gatgttcacg
     3961 ctgacctcct tccttttcag cctgaacagt tatcacgaat acagccggca atcgtcggcc
     4021 atcatattca caccgaccgt cactgtaaag agcagcccca cagatgggag tgtggattta
     4081 ttcgtgatcc atgaagggac gaaagtcttc attaccgacc gggtggatga ctggagcgaa
     4141 gtcaggctgg tcaatggcaa cgtaggctgg atcaagacga atacgttccg cccgatttag
     4201 cttgatctgc agcggaaaca ggcagtttgc aattttgcgc aggcctgacc tcacaggccg
     4261 tttcctgatt ttcacttgcc ggatactgat caaaccacca atcttatcct tttaatttta
     4321 aatttgtatc acttaacaag ggcaggatat tataaatatc tttgttaaaa ttttaactaa
     4381 gccggatttg cacgtatata aaaatagtgt aaattgggcg actgttttta taaggacgga
     4441 ttacaataaa tcaaactcta tttatatgaa taaaagacct ttaccctttg gctactttat
     4501 tttaacaatg gccttgtcgg cttttttgat ctctgcgacg cttaccgggg atgaaaaaat
     4561 agaagccagg caggagaccg aatcggatta tcaggccagg atcaggaaca accagttgac
     4621 cggtcagatc actccggccg attacctgaa ggcgcagcaa caaatcagag aactggcagc
     4681 caacagatct ggcggtgcgt atgattttga ctggcagttg ctcggtccga acaacctggg
     4741 cggccgcacc cgggccattt tgtttgacaa ccgcgatgct acgggtaaca ccgtttacgc
     4801 cggaagtgtt ctgggtggtt tgttcaaatc cgataaccag ggcagcaaat ggtataaaat
     4861 agacgaagcc aatggaggcc tttacgtcag ctgtatcaca caggcaggca acggcgacat
     4921 ttacgttggt actggtgaag gattcaccgt tcagcaatac acagtattcg gcgaatggag
     4981 atattccgga gggctgatgg gccaggggat tttcaaatca accgacgggg tgaattttac
     5041 ccgtctggcg tcaaccaatc ctacgattaa cggcaataac gaactggaat ggggcttcat
     5101 caacgaactg gctgctcacc cttcgaatgg cgcattgtat gcttccacca acaccggtct
     5161 taaatactcc tccgatggtg gaaacaactg gcaggctgca aagatggccg acggaacgat
     5221 cctgaacctc tcgtcaggcg atgtaaaaat ggcagcaaac gggttagtgg ttgccgaagt
     5281 gagcaacaag tgctatgtat cggaaaatgg caacttcgac aatttccagc ttcgttccgg
     5341 caaagccagt tttaatcttc ccgacaccag cgccatcggc aggatcgaat ttgccatcgc
     5401 cccggccaac aatgatatta tttatgcact ggttgtaaaa accagtggcg cgctttacaa
     5461 tgtctaccgt tcggacgaca agggggcgac gtggtcggtt gtgggcccgg gcggaagcgc
     5521 caatttcaat gttttcaata caggaacggc ctccgctacc ggtaacgggc tttttgccgc
     5581 tacgatcgaa gtatacccgg atgatccgta ccatgtgctt atcggaggtt atgacctctg
     5641 ggaaggaaaa aagatccagg acgacggttt ttaccagtgg gtcctgcggt ccacctcttc
     5701 agccaactgg ctgtcggtgg ctttcctgtg gcaggggcat catgtttacc ggtttgctcc
     5761 gggttcatcg gaaaaagtgt taatcggtac caacgggggc atctccctgg gattgcttga
     5821 tccggaattt ttccagttcc agtttatgaa caaggattat atcgccagcc agttttacac
     5881 ggtcagcccg accatggaga aaaaagatgt cgtcggcgga gcccaggata tcggaacaat
     5941 ttatatccgc ggatctgtca acccggccga cagcaggagg ggcgaagata tctggactac
     6001 ccagagcaat gtcccggatg gccgaaccgg aggttattgc gcatggtcga ccatctatcc
     6061 gacagcggtc atttattccc ggttccctca gcccgaaggc tcaccggaaa cttttatacg
     6121 ccgcaatgaa ttcagcggag gacccgactg ggcacttaac aacacattca caacggctta
     6181 cctgagcact tcattcctgc cacccttcgt gctgtatgaa aattttgagg attataacag
     6241 taaagattca acagaattca aagctaatcg cgatctcgcc gccggcacta ccgtttgggt
     6301 agaaagcaac aacggcaaac ggcctttcaa acatgtgctc gaagcagcca tcgattcaag
     6361 tgagaccgtg atggtcaaag acattatctc cacccggttc tttatcggtg gcgataaccg
     6421 cgtgatgatg accaaacaga tgatccagtt cgacgtaagc cccgaatggt ttacgatcag
     6481 cgactcgctg cgtggcggcg tcaggggcaa accgcaggca atggcctatt cttcggatgc
     6541 caaccacctg ttcgtcggca ctcagaaagg caaactttac aggatctcca atatcaagta
     6601 tgcctataat aatgaaaccg ctgatgtttc cagcccttac tgcgtcattt ccaccaaaca
     6661 aatccccgtg tacctgcccg gaactacaac agaaatcacc caggtgatca cctctgttgc
     6721 agtcgatccg aacaatgatg aaaaggtaat tatcactctg ggaaattatg gaaatgaaac
     6781 ctatgtgtat tacaccgaaa acgccctgga tgaaaacccg gtattccact ctgcacaggg
     6841 taatctgccg caaatgccgg cctattcttc cctgatcgag atggatgccg ccaaccactt
     6901 ggtgtttgtc gggacggagc atggtatcta cgttacgaat gatattggat catccaatcc
     6961 aacctggacg gctgaaaacg caaacatcgg tgcagtgcct gttttcatgc tgaaacagca
     7021 aacgatccgg aaaacaaacg atacactggt ctttgtcaat atcgacacga cctacctggt
     7081 gcattacggg acaaacaact atggagtta
//
LOCUS       DAOQXS010000077         7087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_10983_length_7087_cov_3.476251, whole genome shotgun sequence.
ACCESSION   DAOQXS010000077 DAOQXS010000000
VERSION     DAOQXS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_7087_cov_3.476251"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKM34_03435"
     CDS             <1..855
                     /locus_tag="PKM34_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNQ82669.1"
                     /translation="YTLRENGYSTVYFTTHDGQFDNIEGFLKGNDFEYIFTQDDYPAA
                     KVKTSLGVPDDYMFEFSMPVIDGLYEMNKPFFITFLTASNHTPYYLPGYYQPRHKDIK
                     DQMVEYADWSLRKFVEMAASKPWFEKTVFVFIADHGLPINARYDIPLDYHHSPLIFYA
                     PGIIRPETFSVTGGQIDVFPTIMGILNQNYINNTLGIDLIKENRPYIFINHEDKLGVL
                     DDEFLLIMKPEGERLLYKYKSGDKKNCSNDYPDQVRSMEEYTNANLQVFQDLMGSDKR
                     FCDRKSDD"
     gene            1054..1887
                     /locus_tag="PKM34_03440"
     CDS             1054..1887
                     /locus_tag="PKM34_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNQ82670.1"
                     /translation="MKALLKFLLVIILIPVILLLVLFFVEGGTGRKEPVTLPVKDPDA
                     PLYFAHRGVATDLPENSLPAIEKARKVGFRALEVDIRKTSDNELILFHDANAGRLLGI
                     DTTVASLSLAEIRQRNLLWNGDTTKFKVLTLKELLDKYQDNFIYYFDMKLGGIADIDE
                     LVHLVWSYDISKNIIIGSTSVVAVLYIEYQYPAINTALEGFHPGTQNVWYLIPKNLKP
                     DFYSGFARHVDEEHVNWMQKNDLLQQRIVYEADSTNYQKMLDFGINKMIIDHWPELQV
                     Q"
     gene            2079..3146
                     /gene="thiL"
                     /locus_tag="PKM34_03445"
     CDS             2079..3146
                     /gene="thiL"
                     /locus_tag="PKM34_03445"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNQ82671.1"
                     /translation="MTTNDHIPPEHRTELSELGEFGLIDLLTKEITLMNQSTVYGVGD
                     DAAVIDNKDKLTLVSTDLLVEGVHFDLSYTPLKHLGYKAAVANISDIAAMNGIARQLL
                     VGLAVSSRFPVEALEEIYAGIRQACKVYHVDLSGGDTTSSSKGLFISITVIGEAAKDE
                     VVYRHTARENDLICVTGDLGGAYMGLLLLEREKAAFQGDPNLQPDLEGFDYILGRQLK
                     PEPRTDIIRLLKEIHVKPTAMIDISDGLGSEILHICKGSKLGCRIYEEKIPIDPTTVN
                     MAGEFGIQPVTAALNGGEDYELLFTIRQEDFEKIRTIPNVSVIGHMTESAAGAHIVAP
                     DGVLVEITAQGWNALKKNVEF"
     gene            3230..5014
                     /gene="uvrC"
                     /locus_tag="PKM34_03450"
     CDS             3230..5014
                     /gene="uvrC"
                     /locus_tag="PKM34_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNQ82672.1"
                     /translation="MDTSLVLRTLPDRPGVYQFYDQEGKLLYVGKAKNLKKRVSSYFN
                     REHFGGKLNMLVRKTAEIRHIVVGSEFDALLLENNMIKEHQPRYNVMLKDDKTYPWIC
                     IKNEPFPRVFPTRTIVKDGSLYFGPYASVKMMNTLLELIRQIYPLRTCSLKLTKQNID
                     AGKFSVCLEFHLGNCLGPCAGLQAEENYMAAFAGIREMIKGNFSGVLKELRARMESHA
                     QAYEFEKAQAVKEKISLLERYQAKSTIVNPKLGEMDVFSIADGEEEAYVNYMKVANGA
                     VIQAHTVELKKKLDESLEELLAYVVFNLRTRLASEAREILVPFELDLELPGVILTVPK
                     IGDKKHLLLLSEKNAKYYKLEKEKQLDLVDPERKVNRIMATMMKDLRMKEPPVHIECF
                     DNSNFQGDQAVSAMVCFRNGKPDKKEYRHFNVRTVAGPDDFATMEEVIMRRYKRLVEE
                     AKPLPQLIVVDGGKGQLSAALKSLEELGLRGKITIIGIAKKLEEIYYPDDPLPMYIDK
                     KSETLKILQRLRDEAHRFGITHHRKKREKATLKTELTEIRGIGEETAKKLLSDFKSVK
                     NIKNAPVEELEKSVGKAKAGVVFMYYHS"
     gene            5202..6035
                     /locus_tag="PKM34_03455"
     CDS             5202..6035
                     /locus_tag="PKM34_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82673.1"
                     /translation="MKTSKILLMALLLVSNGLGAQKNSIDRYLSLKPSEIKARKAEIQ
                     EYAVTLKWQNLDAIHGNKFNCDAVSATYIVGLENDSVSWKDVSSAQISDFLQTDYSGA
                     SLPSFNGFTYKINETNFLNEDFYESIPVEQRDLAKWLVSDAVQMQGLAWYIFDSLEFN
                     KPFQMKFLNNYEIKFEDWVTFTSRYQKFIWSGITKYNNEVCAIIRFESLYNPLKIDNS
                     QMSVLGRALYYGEMWISLEDKQVEYATMVEDDVLKLKSPLFPEEQLIDLQREVIFKKK
                     E"
     gene            6054..7085
                     /locus_tag="PKM34_03460"
     CDS             6054..7085
                     /locus_tag="PKM34_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ82674.1"
                     /translation="MKRKIIFGAIIFSIAFAVLFSLMYKWGQTGNPFRAETVVYGAVI
                     LLNLLITGSVGYVIFRKFSKKASGQLEKFIIPAGISFVLFALIVSLSLVSLGVYLLYL
                     IRGNDMTNFAGQLFTVELVSALKQFSAWILIGSALFFYMIWRKAIEREQLLREENLKY
                     KYRNLKSQVNPHFLFNSLNTLSELVYEDAKRADDYIQKLSGIYRYVLENEETDLISLK
                     EELDFIRQYFILQKARDQEKIFLEIDIDREDEYRIIPVSLQLLVENALKHNSMSRENP
                     LRIKIFIDDDDIIVSNNIQRRNILNNSTRMGLSNLKERVRLIMGRELIVIEEPDQFVV
                     KLPVNSMLR"
BASE COUNT         1954 a         1605 c         1768 g         1760 t
ORIGIN      
        1 tacaccctcc gagagaatgg ttattcgacg gtttatttta ccactcacga cggccagttc
       61 gacaatatcg aagggttttt gaaagggaat gattttgaat atatttttac ccaggatgat
      121 tatccggccg cgaaagtcaa gacctccctg ggtgttcccg acgattatat gtttgagttc
      181 tcgatgccgg ttatcgacgg gctgtatgag atgaacaaac cctttttcat taccttcctg
      241 accgcaagca accacacccc gtattatctg cccggttatt atcagccgag gcacaaggat
      301 attaaggatc agatggtgga gtatgccgac tggtcgctca ggaaattcgt ggaaatggca
      361 gcgtcaaaac cctggtttga gaaaactgtc tttgttttca ttgccgacca cggcctgccg
      421 atcaatgccc ggtatgatat cccgctggat tatcaccatt ctccgctcat cttttatgcg
      481 ccggggatta tccggccgga gacgttttcc gttaccggcg ggcagatcga tgttttccca
      541 actatcatgg gaatccttaa tcaaaactat atcaacaata ccctgggtat cgatcttatt
      601 aaagaaaaca ggccttatat ctttatcaat catgaagata agctcggtgt tttagatgat
      661 gagttcctgc tgatcatgaa accggaaggg gagcggttgc tttacaaata taaatccggg
      721 gataaaaaga attgcagtaa cgattacccg gaccaggtgc ggtcaatgga agagtacaca
      781 aacgccaatc tgcaggtatt ccaggattta atgggaagtg ataagcggtt ctgcgacagg
      841 aagtccgacg actaaagccg tcggttctaa cgacccctga accaacggct ttagtcgttg
      901 gagcacgatc acaaacaacc aacggcttta gtcgttggag cacgatcaca aacaaccaac
      961 ggctttagtc gttggagcac agattattaa taactcgtgt ttaacgccaa aattttcccg
     1021 gatcaaggat tgaattttgt aattttactc ccgatgaaag cactccttaa atttttactg
     1081 gtaatcatcc tcatccctgt tatcctgctt ttggtattgt tttttgttga ggggggtacg
     1141 gggagaaaag agcctgttac ccttcctgta aaagatccgg atgctcccct gtattttgcc
     1201 catcggggcg tggcaacgga tttgccggag aacagcctgc cggcaataga gaaggcccgt
     1261 aaagtcgggt tcagagcgct agaggtcgat atccgcaaaa cttccgacaa tgaactgatc
     1321 cttttccatg atgcaaacgc cggaaggctc ctgggaatcg atactacggt tgcctccctg
     1381 tcactggcag agatccggca aagaaacttg ctgtggaacg gggataccac aaaattcaaa
     1441 gtattgaccc tgaaagaact gttggataaa taccaggata atttcatcta ttattttgat
     1501 atgaagctcg gaggcatcgc cgatattgat gaactggtcc acctggtatg gagctatgat
     1561 atttctaaga atatcattat tggcagcacg agcgtggtgg ccgttttgta tatcgaatac
     1621 cagtatcctg caataaatac tgcgttggaa ggattccacc cgggaaccca aaatgtctgg
     1681 taccttatcc cgaaaaacct caaacccgat ttttactccg ggttcgcccg gcatgtcgat
     1741 gaagagcatg tcaattggat gcaaaagaat gatttgttgc aacagcgtat cgtgtacgag
     1801 gccgacagca caaactacca aaagatgctt gatttcggca tcaacaagat gatcatcgat
     1861 cactggccgg agctgcaagt gcaatgattt aaaatagcgc gacaatatat ctcaaccact
     1921 aaggcgacga agggtcttca cgaagcgcac caaggtaagc tattaataat caattccttt
     1981 gtgttccttt gtgaatatcc tttgtgtcac tttgtggtta aacccagatt tatatacttt
     2041 tacaaccaat gaccatcaat gactatcaat gaccatcaat gacaacaaat gaccatatcc
     2101 cccctgagca tcgcaccgag ctgtccgaac tcggtgaatt cggccttatc gatcttctga
     2161 ccaaagaaat tacactcatg aaccaaagca cggtctatgg agtgggtgac gatgccgccg
     2221 ttattgacaa caaggacaag ctgacgttgg tttccaccga tttgctggtt gagggcgtcc
     2281 atttcgacct gtcgtatacg ccgcttaagc atctcggcta caaggcagca gtggctaaca
     2341 tttccgacat tgcagctatg aacggcattg cccggcaatt gctggtcggt ctcgcggttt
     2401 ccagccggtt tccggtggag gcgctggagg agatatacgc cggcatccgc caggcctgca
     2461 aagtctacca tgttgacctc tccggcggcg ataccacgtc cagctcaaaa gggctgttca
     2521 tctccatcac agtcatcggc gaagccgcga aagatgaggt ggtttaccgc cacacggccc
     2581 gcgaaaatga cctgatctgt gtgaccggcg acctgggcgg tgcctatatg ggactgttgc
     2641 tgctcgaaag ggaaaaggcg gcattccagg gcgatcccaa tttacagccc gacctcgaag
     2701 gctttgatta tatcctgggg cgccagctta aacccgaacc ccggaccgat atcatacgtc
     2761 tgctgaagga aattcatgtg aaaccgaccg ctatgatcga tatatccgac ggactgggat
     2821 cggagatcct gcacatttgc aaaggctcga agctgggctg ccggatctat gaggaaaaaa
     2881 ttcctatcga cccgacaacg gtcaatatgg ccggtgaatt cggtatccag ccggttaccg
     2941 ccgccctcaa cggcggagaa gattacgagc tgctgttcac catccgccag gaggattttg
     3001 agaagatcag gacgattccg aatgtttcgg tgatcgggca catgacggaa tccgcggcag
     3061 gcgcccatat cgtagcccct gacggggtgc tggtggagat cacagcgcag ggatggaatg
     3121 cacttaaaaa gaatgttgaa ttttgaatgt tgaattttga attcgggtaa ttttataata
     3181 attaatcaat cattcaacat tcaacactca acattcaaca ttaatctgta tggatacttc
     3241 tctcgttctg aggactttgc ctgaccgccc cggggtttac cagttctacg accaggaggg
     3301 aaagctgctc tatgtcggga aagcgaagaa cctgaaaaag cgtgtttcgt cgtatttcaa
     3361 ccgggagcat ttcggcggga aactgaatat gctggtgcgg aaaaccgccg agataaggca
     3421 tatcgtggtg ggatcggagt tcgatgccct gttgctggag aacaacatga tcaaggagca
     3481 tcagccgcgc tacaacgtaa tgctgaaaga cgacaagacc tatccctgga tctgcattaa
     3541 aaatgagcct ttccccaggg tgttcccgac gcggacgatc gtgaaggacg gttccctcta
     3601 tttcgggccg tatgcttcgg taaaaatgat gaatacgctg ctggagctca tccgccagat
     3661 ctacccgttg cgcacctgca gcctgaaact gacgaagcag aatattgatg cgggaaaatt
     3721 cagcgtctgt ttagaatttc acctgggaaa ctgcctcggg ccctgtgccg ggttgcaggc
     3781 ggaagagaac tatatggccg cttttgccgg catccgggag atgatcaaag gcaacttcag
     3841 cggggtactg aaggaattgc gggcgagaat ggagagccat gcacaggcct atgaattcga
     3901 gaaagcgcag gccgtcaaag agaagatctc cctgctggag cgctaccagg ccaaatcaac
     3961 gatcgtgaac ccgaagcttg gggagatgga tgttttttcc atcgccgacg gggaggaaga
     4021 ggcgtatgtc aactacatga aagtggccaa tggcgcggtt atccaggctc atacggtaga
     4081 gctgaagaag aagctcgacg aatcactgga agagctcctg gcgtatgtgg tttttaacct
     4141 gcgcacccgg ctggccagcg aagcccggga gatcctggtc ccgtttgaac tcgacctgga
     4201 attgccgggc gtgatcctca ccgtgccgaa gatcggggat aaaaagcacc ttttacttct
     4261 ctccgagaag aacgcaaaat attataagct cgaaaaagaa aagcagctgg atctggtcga
     4321 tccggaacga aaagtaaacc ggatcatggc cacgatgatg aaagacctgc gaatgaagga
     4381 gccgccggtc catatcgaat gtttcgataa ctctaatttc cagggcgacc aggcggtatc
     4441 ggccatggtg tgtttccgca acgggaaacc cgataaaaaa gaataccggc atttcaacgt
     4501 gaggacggtt gcagggcccg atgattttgc cacgatggaa gaggtgatca tgcgtcgcta
     4561 taaacggctg gtggaagaag caaaaccctt gccccagctc atcgtggtcg acggcgggaa
     4621 agggcagctc agcgccgcgc tgaaaagcct ggaagagctt gggttgcgcg gaaagataac
     4681 catcatcggc atcgctaaaa aactggaaga gatctattat cccgacgatc ccctgccgat
     4741 gtatatcgat aaaaagtcgg agaccttaaa gattttgcag cgtttacgcg atgaagccca
     4801 ccgcttcggg atcacccatc accggaaaaa aagggagaaa gccactttga agaccgaact
     4861 gaccgaaatc cggggaatag gagaggagac ggcgaaaaag ctgctttccg acttcaagtc
     4921 ggttaagaat atcaaaaatg ccccggtgga ggaacttgag aagtcggttg ggaaggctaa
     4981 ggcaggggtg gtgttcatgt attaccattc ttagggcagt cggcagtcat tcctttttcc
     5041 ctttttcttt tttctttttt ctttttccct tttccctcac aaccggcccg attccggtaa
     5101 ttgacgactc agccatgaaa atcagcaact ctgcaacttt gattttttaa tcggaatccc
     5161 atccgcttct tttgctgatt attaacttaa attttctaaa aatgaaaact tcaaaaattt
     5221 tattaatggc tttattgctg gtatcaaatg gcctgggggc tcaaaaaaac agtatcgacc
     5281 ggtatctctc tctcaaaccg tctgagatca aagcgcgaaa agcggaaatc caggagtatg
     5341 ctgttacact gaaatggcag aacctggatg ccatccacgg taataaattc aattgtgatg
     5401 cggtcagcgc aacctatatc gtgggactgg aaaatgattc ggttagctgg aaagatgtca
     5461 gttcagcgca aataagcgat tttctgcaga cggattacag tggggccagc ctgccgtcgt
     5521 tcaacggttt cacttataaa atcaacgaaa cgaatttcct caatgaggat ttttatgaat
     5581 ctattccggt tgaacaaagg gacctggcaa agtggcttgt gtcggatgca gttcaaatgc
     5641 agggattggc ctggtatata ttcgacagcc tggaattcaa caaacccttt caaatgaaat
     5701 tcttaaataa ttatgagatt aaattcgaag attgggtaac ttttacgagc cgttatcaga
     5761 aattcatctg gtcgggcatc acaaaataca ataacgaggt gtgtgctata atcaggtttg
     5821 aatctttata caaccccctc aagatcgaca acagccagat gtcggttctg ggccgggcgt
     5881 tgtattacgg ggagatgtgg atatcgctgg aggataaaca ggttgaatat gccaccatgg
     5941 tcgaggacga tgtcttaaaa ctgaagagcc cgttgttccc ggaagagcag ttgatcgacc
     6001 tgcaaaggga agttattttt aagaaaaagg aataactgct atatttgacg gaaatgaaac
     6061 ggaaaataat attcggcgcc ataatctttt ccatcgcctt cgcggtactg ttctcgctga
     6121 tgtataaatg gggacagacc ggcaatccgt tcagggcaga aacggttgtg tacggtgcgg
     6181 tcatcctgct gaacctgctg ataaccggga gtgttggcta tgtcatattc cggaagttct
     6241 cgaaaaaggc atccggacaa ctggaaaaat ttatcattcc ggcgggcatc agttttgtgc
     6301 tgttcgcgct gatcgtttca ctttccctgg tcagcctggg agtctatttg ctttacctga
     6361 tccggggaaa tgatatgacg aattttgccg ggcagttgtt tacggtcgag ctggtcagcg
     6421 cgctgaaaca gttttcggcc tggattctga tcggctctgc tctgtttttt tacatgatct
     6481 ggcgtaaagc cattgaacgg gaacagttgc tgcgggaaga aaacctgaaa tataaataca
     6541 ggaacctgaa atcacaggtc aatcctcatt tcctttttaa cagcctgaat accctttccg
     6601 aactggttta tgaagatgcg aaaagggcgg atgattacat acagaagcta tccggtatct
     6661 accggtatgt tctggaaaac gaagaaaccg atctgatctc cctgaaggag gaactggatt
     6721 tcatccggca atattttatc ttgcaaaaag caagagatca ggaaaaaata ttcctcgaaa
     6781 ttgatatcga cagggaggac gaataccgga tcatacccgt atccttgcaa ttgttggtcg
     6841 agaatgcctt aaagcataat tccatgtcac gtgaaaatcc gctgaggatt aaaatcttta
     6901 tcgatgacga tgatattatc gtttcgaata atatacaacg aaggaatatc ctgaataact
     6961 caacccggat gggactgtcg aacctgaagg agagagtacg tttaattatg ggcagggaat
     7021 tgatcgttat tgaagaacca gatcagtttg ttgttaaact accggtaaac agtatgttaa
     7081 gatgaaa
//
LOCUS       DAOQXS010000078         7062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11036_length_7062_cov_3.868845, whole genome shotgun sequence.
ACCESSION   DAOQXS010000078 DAOQXS010000000
VERSION     DAOQXS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11036_length_7062_cov_3.868845"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1436)
                     /locus_tag="PKM34_03465"
     CDS             complement(66..1436)
                     /locus_tag="PKM34_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82675.1"
                     /translation="MAISKSKIIAISVFIISFVVFYAGFRYNTWQVLKNGKFLDNQAE
                     SLVVGRLVKSAADGITSSGGLCGRCYNQKNDTALFENQYRAFLNNESCKKYVPYLSQI
                     GFHAMVYFAIDKASPFKKSTDLQIMYAFKSGMLAMVMSFIVLWFLLELGLLPAIFVSA
                     GILLTPWFTFLGRDLWFCIWTNFLPFLAALFILRQENRTGKPNGRSILLIVSLVVLFS
                     FIYNGFEWVTTTLIMTAVPFFYYWRMGQWPFARLAKRIGWLVAGSLASVMITFSILIY
                     QIAQVKGTISKGIEWLIFSFQKRTYGGGEDLPEVFEKQIDHSIWEVFLINLGGVAFPF
                     PAFISQHVPRYFGGILFAEILFILIIITCLILYRRNYLKIKEENRKWLQNLSIVTWIS
                     LLAPFSWFVIFKGHAWSHHHINYITWWMPFGLFVMALLGSFFKVWVANRFIGSEAVSK
                     ALRATS"
     gene            1459..2865
                     /locus_tag="PKM34_03470"
     CDS             1459..2865
                     /locus_tag="PKM34_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ82676.1"
                     /translation="MYTKDDIFLYFEKLAPKRLKWKKRNRFYHSLLEKYYRFLVPEGS
                     KVLEIGCGTGDLLHAVKPTQGVGVDISPAMVGIAGDRYPGLAFYTQDAESLSLDQRFD
                     YVIMSDLLGSLHDIQQAFACLKNVTDRNSKIIITQYNFLWEPALRFLEFSGLKARQPR
                     QSWLSMHEIRKLLELEGFECFKSFRKILLPLRIPLVATFFNKFLANLPLLNHLCLVNF
                     IVARRDEPVEEELSVSIVVPARNEKGNIENAILRTPGFGRWQEYIFIEGHSSDGTYEE
                     MLRVKEKYPELRIKTAIQPGRGKADAVRLGFVMAEGDILMILDADLTTPPEDMPKFYL
                     ALSRNRGEFINGSRLVYPMEDEAMRLLNLLGNKFFSLFFSYLLDQQLKDTLCGTKVLR
                     RTGYEKIKANRKYFGDFDPFGDFDLLFGASKLNMKIVEIPVRYRNREYGSTQISRFRH
                     GWLLIRMSLFAAMKIKFL"
     gene            complement(3270..3521)
                     /locus_tag="PKM34_03475"
     CDS             complement(3270..3521)
                     /locus_tag="PKM34_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82677.1"
                     /translation="MELGAIFKTTDDPQMNIPRILITGIGKSGSTALFYSILKALPDC
                     TIQLFEPDNIKKTLPSDTPPPALIKSFIPYSENFSFFEK"
     gene            complement(3534..5189)
                     /locus_tag="PKM34_03480"
     CDS             complement(3534..5189)
                     /locus_tag="PKM34_03480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ82678.1"
                     /translation="MEEVIAASPVPAIVPTEKKYINDYFDKIFVINLDRRPDRKANLL
                     QKLYELNIEAAFIEAVDGYTDENINEFNRYNSIPYTHPDAHPLEIKHQRKMIMSPGAW
                     GVLKSYHKVIALAKEKGYRRILVFEDDIIFIEDFHARFANFIRSIGSDWKIICLGASQ
                     HVWNYGTTFTYYEEGANTYDPAKPYYHPIKTDGAFAVGYDHSIYDLLQEEIAKMNCAY
                     DSGPMRKVFQTYTDKCYVAQPNLVIADVSQSDIGENRQQEQLAERLKWNMQLYDTKFK
                     PELISVIMPAFNASATIRKSIQSLQAQSYSNIEIIVVDDGSTDDTVSIVEEIASKDNR
                     VKLIRNEQNRGCYFVRNEGLRAARGKYIAINDADDIALNHRLARQVIPLLSGDAEFTI
                     GKILRSRCSIEELEPEKENEMLKLVYSKRVKNPEGKYAYADKEILALGTSMFTRNFFE
                     KHGLFWEERHSADAEIFERYFYNKYGILFKEGELNVHTFLFYCDPIPRSYKRMDDLLL
                     ICPALRETNLTKKYPIRSEERNLFEKNWRARLIGNYTYDYPRF"
     gene            complement(5389..>7062)
                     /locus_tag="PKM34_03485"
     CDS             complement(5389..>7062)
                     /locus_tag="PKM34_03485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ82679.1"
                     /translation="TGFEKENNWLIYHRNSSNLKFARSCNEGAKVARGQFIVFLNNDT
                     LVTINWDKILLDTIENDRDTWMVGAKLLYPNSMIQHAGVYLPELTGVSFGHVYNGFPS
                     DFPPANIGKEVQCVTAACMIMRREDFLAIGGFDTGFINGSEDIDLCLRIVDKGKKIVY
                     QPQCEIIHLESKSEGRFDQSSQNSARLFERWKGKVRSDLVDLMEQDLKTCENNGQLIL
                     ISREKINLQAPVMKFSFAIPSRDLLVGNGLVIGLGCIATEKSIIRLSYSTRSGYSDDK
                     ILNQTQPVYKGNNLALFLLKVDLLKDLSSFIVEGGEPGIQITSLSYYVFPYSIKKQTT
                     LTILHQQDTDQENLPAIADEFENNFKGNEIRMTTLESGLSASSLNTAISLSDTEYILV
                     IGNGMKVNPVFPVRAIEILELQPKVGFVIGDITVHDSNTAKLVKPDFNPTTLLSKGKT
                     GIYGLFRKKCWELAGGYHDAHPLLLDTDLFLGILETAIWRGYKINDQAFEIFLGRESF
                     RINYQGERDRIRARHIRYLFREYNKKIAILQKKEEKARATETGEGTESV"
BASE COUNT         1822 a         1621 c         1634 g         1985 t
ORIGIN      
        1 cgtgccttct tcgtgatcct ctgtgtaaca atcatttata aattacacga agttccacga
       61 aggtttcacg aagttgcacg aagggctttt gagacagcct cactaccaat aaatctatta
      121 gcaacccaga ctttgaagaa agatccaaga agcgccatca caaacagccc gaaaggcatc
      181 caccaggtga tgtaattgat atgatgatgg ctccaggcgt ggcctttgaa gatcacaaac
      241 catgaaaacg gcgccaggag cgatatccag gtgacaatgg aaagattctg caaccacttc
      301 ctgttttctt cttttatctt taaataatta cgcctgtaca agatcagaca agtaataata
      361 atgagaatga agaggatctc cgcaaacaag atccctccga aatacctcgg aacgtgctgg
      421 ctgatgaatg ccgggaaggg gaatgccaca cctcccaggt tgatgaggaa aacctcccag
      481 atggaatgat caatctgttt ctcaaacact tccgggagat cctcaccccc tccgtaagtg
      541 cgcttctgaa aagaaaagat gagccattcg atccctttcg aaatcgttcc tttgacttgt
      601 gcaatctggt agatcaaaat agagaaggtg atcatcaccg aagccaggct gccggcaacg
      661 agccagccga tccgctttgc cagcctggca aagggccatt gtcccatccg ccagtaatag
      721 aaaaacggta ccgcagtcat gatcagcgtg gtggttaccc attcgaaacc gttgtaaata
      781 aagctgaaca gaacaaccag gctgacaatc agcaaaatag atcgtccgtt aggttttccc
      841 gtcctgttct cttgtcgcag gatgaaaagt gcggcaagga aagggaggaa attggtccag
      901 atgcagaacc acaggtcgcg gcccaggaaa gtgaaccagg gcgtcaggag gatgcctgct
      961 gatacgaaaa tagccggcag cagccccaac tccagcagga accacaggac aataaagctc
     1021 atcaccatgg ccagcattcc cgatttgaaa gcatacatga tctgcagatc ggtgcttttc
     1081 ttaaacggag aggctttatc gatggcaaag taaaccatcg catgaaaacc gatttgcgac
     1141 agatagggaa cgtatttttt gcaggattcg ttattcagaa atgcccggta ctggttttcg
     1201 aaaagcgcgg tgtcattttt ctggttataa caacggccgc aaaggcctcc ggaagaggta
     1261 atgccatcgg cagccgattt gacaagccgt cccacgacaa ggctttcggc ctggttatcg
     1321 aggaattttc cgttcttcag tacctgccag gtgttgtacc tgaacccggc ataaaaaacg
     1381 acaaagctga tgatgaaaac cgatattgcg attatctttg acttgctaat tgccatgggt
     1441 aagacgaatt aatccgttat gtatacaaag gacgacattt ttctttattt tgaaaagctg
     1501 gcgccgaaaa ggctgaagtg gaaaaaacga aaccgttttt atcattcgct gctggagaaa
     1561 tattaccgtt tcctggtacc cgaaggcagc aaagtgctgg agataggttg cgggacgggc
     1621 gatctgctgc atgccgtaaa acctacgcag ggagtcggcg tcgatatttc gccggctatg
     1681 gtcgggatag ccggggatcg ttatcccggc ctggcttttt acacccagga tgcggaatcg
     1741 ctttcgcttg accagcggtt cgattacgtg atcatgtcgg atctgctggg cagccttcat
     1801 gatatccagc aggcttttgc ctgtttgaaa aacgtcaccg accggaacag caagatcata
     1861 atcacgcaat ataatttcct gtgggaaccg gcgctgcggt tcctggagtt cagcgggttg
     1921 aaagcacggc aacccaggca gagctggcta tcgatgcatg agatcaggaa attgctggag
     1981 ctcgaagggt tcgaatgttt taaaagtttc cggaaaatac tgctgccgct tcggataccc
     2041 ctggttgcca cgtttttcaa caaattcctg gccaatctcc ctttgctgaa ccatctctgc
     2101 cttgtcaact tcattgtggc caggagggat gagccggtcg aagaggagct ttccgtttcg
     2161 atcgtcgtgc cggcccgcaa cgagaaaggg aacatcgaaa atgccattct ccggacgccg
     2221 ggatttggcc ggtggcagga gtatattttc atcgaagggc attcttcgga tggcacctac
     2281 gaggagatgc tgcgggtgaa ggagaaatac ccggagctcc gcatcaaaac ggccatccag
     2341 ccgggccggg ggaaagccga tgcggtacgg cttggttttg tgatggcgga gggcgacatc
     2401 ctgatgatcc tcgacgccga cctgaccacc ccgccggagg atatgccgaa gttttatttg
     2461 gctctcagcc ggaaccgggg tgagttcatc aatggcagcc ggctggtcta cccgatggag
     2521 gacgaggcga tgcgcctgct gaacctgctc gggaacaagt tcttcagcct gtttttctcc
     2581 tacctgctcg accagcagct taaagacacg ctatgcggca ccaaagtgct ccggagaacg
     2641 ggctacgaga agattaaagc caaccggaaa tatttcggtg atttcgatcc gtttggcgat
     2701 ttcgacctgt tgttcggggc cagcaagctg aatatgaaga tcgtggagat acccgtaagg
     2761 taccgcaacc gggagtacgg ctccacccag atcagccgct tcaggcacgg atggttgctg
     2821 atccggatga gcctttttgc ggcaatgaag atcaagtttc tttaagaaga aagtaaacgg
     2881 aaggatccag ttttggttgt tcttccaatt gccattcatc cgcatagccg aatttgtcca
     2941 tgtaactttt catcattgga cgttaaaagt caatgtcttc cggggtgagc cagtttctcc
     3001 aatcgcagta ttttttatgc cgggccaccc tttcaaaatt cgacgggatt tcggtcccgt
     3061 tgtcaagctc tagtcccagg tagtttttag ggccaggcta cagacggaga tctcatgctg
     3121 accggtttcc ttttgcttaa tactatcaag aaatgagcgg atcagttgaa cagctatttc
     3181 cttttctccg tggaaaactt cctgatgata ttgtagggtt tgtaaagcag caggatgagg
     3241 ttgtcgcgcg gatcgcggac cagcaggatc tatttctcaa agaagctaaa gttttccgaa
     3301 taggggatga aactttttat gagtgccggg ggaggcgtgt cggaaggtaa agtctttttg
     3361 atgttatccg gttcaaaaag ctgtattgta caatccggca gcgctttcag gatggaatag
     3421 aacagggcgg ttgaacccga tttgccgatg ccggtgatga ggatgcgggg aatgttcatt
     3481 tgaggatcat ctgttgtttt aaagattgca cccaattcca taatttatat aggttaaaac
     3541 cgcggataat cataggtata gtttccgatc agcctggccc gccagttttt ctcaaaaagg
     3601 tttctttcct cggatctgat cgggtatttt tttgtcaggt ttgtttcccg gagtgccgga
     3661 cagatcaata ataaatcatc cattcgctta taagaacgcg gaatggggtc gcaataaaat
     3721 aagaaggtat gaacattaag ttctccttct ttaaataata tgccatactt gttataaaaa
     3781 taccgttcaa atatttccgc atcggctgaa tggcgttctt cccagaacaa tccatgcttt
     3841 tcgaaaaagt tccttgtaaa catagaagtc cccaaagcca gaatttcctt gtcggcatat
     3901 gcatatttcc cttccggatt tttcaccctt ttagaataga ccagtttcag catttcattc
     3961 tctttttccg gttcaagttc ttcgatagag catcggcttc gcagaatttt tccgatagta
     4021 aactcggcat cacctgaaag caaggggatt acctgccggg ccaggcggtg attcagtgcg
     4081 atatcatccg catcgttgat ggcgatatat tttcctctgg ctgcccttaa tccttcgttt
     4141 ctcacgaaat aacagcccct gttttgctcg ttcctgatca gtttaacgcg gttatctttg
     4201 ctggctattt cttccacgat gcttaccgta tcgtctgtac taccatcatc aaccactatg
     4261 atttcaatat ttgaataaga ctgggcctgc aaagattgta tgctttttcg gatagttgcg
     4321 gatgcattaa atgcgggcat gatgacactg atcagttcgg gtttgaattt tgtgtcgtag
     4381 agttgcatat tccatttcag tcgttccgcc agttgctcct gctggcggtt ttccccgata
     4441 tcgctttgag aaacatcagc aataaccagg ttaggctgag ccacatagca tttatccgta
     4501 tacgtctgaa agaccttgcg catgggaccg gaatcgtagg cgcagttcat ctttgctatc
     4561 tcttcctgga gcaggtcata aatcgaatgg tcatagccaa ccgcgaatgc cccatcggtt
     4621 ttgatgggat gatagtacgg tttggcagga tcgtaggtat tcgctccttc ctcataatag
     4681 gtaaatgttg taccataatt ccagacatgc tgactcgctc ccaggcaaat gattttccag
     4741 tcggaaccga ttgaccgtat aaagtttgca aagcgtgcat gaaaatcttc aatgaagata
     4801 atatcatctt caaaaaccag gattcttcta taacccttct ctttcgccag ggcgattacc
     4861 ttgtgatacg atttcaatac accccaggcc ccggggctca tgatcatttt acgttgatgc
     4921 ttaatttcca atggatgggc atcgggatgg gtgtacggaa ttgaattata ccggttaaat
     4981 tcattaatat tttcatctgt ataaccatcc actgcctcaa taaatgctgc ttcaatgttt
     5041 agttcataaa gtttttgcag gaggttcgct tttctgtcgg gccttctatc cagattgatc
     5101 acaaaaatct tatcaaagta gtcattgata tacttttttt ctgttggaac tattgccggt
     5161 acaggggatg cggctatgac ttcttccaat ggtttaatct caacaggctt ttttattttt
     5221 gacgatgagg ttttccatgg ttcaaatgtt acggcgtaag taacgggttc atttggatta
     5281 atagcatgat ccggattaat tgatgagcca gtccggaata aatgttttag ccgatggatt
     5341 gccttattta tagaaatcac ttttctgagt ttaatttatt ttccggtctt agacgctttc
     5401 tgttccttca cctgtctcgg ttgccctggc cttttcttct tttttctgaa gtattgcaat
     5461 cttcttattg tattccctga acaggtatct gatatgcctt gcccttatcc tgtccctttc
     5521 cccctgataa ttgatcctga aagattctct gccaaggaaa atctcgaatg cctggtcatt
     5581 tattttataa cctctccaaa tagctgtttc aagaataccc aggaagagat cggtatccag
     5641 caagagcgga tgagcgtcat gatatccacc tgccaattcc cagcatttct tcctgaacag
     5701 gccatatatt ccggtcttgc cttttgaaag cagtgtcgtt ggattaaaat cgggcttcac
     5761 aagtttcgct gtattgctgt catgcacagt tatgtctcca ataacgaatc cgacttttgg
     5821 ctggagctcc agtatttcaa tggcccggac cggaaaaaca ggattaactt tcattccgtt
     5881 gccgatgacc agaatgtatt ctgtatcgga cagtgaaatg gctgtattca ggctggatgc
     5941 cgacagaccg gattccaggg ttgtcattct gatctcattt cctttaaaat tgttctcaaa
     6001 ttcatcggca atcgcgggca gattctcctg gtcagtatcc tgctggtgta aaatggttaa
     6061 tgttgtttgt ttctttattg aataagggaa tacataataa gacaaagatg ttatttggat
     6121 tcctggttca ccgccttcaa cgataaacga tgataagtct ttgagaagat ccaccttgag
     6181 gaggaataaa gccaggttat ttcctttata aaccggctgg gtctgattga gaattttatc
     6241 gtctgaatac cctgaccggg tgctgtagct gagcctgata atggattttt cagtggcaat
     6301 gcaacccagt ccgatcacaa gcccatttcc aactaaaagg tcacgggaag gaatcgcaaa
     6361 agaaaatttc atgacagggg cctgaaggtt tattttttcc cggctgatca gaataagttg
     6421 cccattgttc tcacaggttt tcaaatcctg ttccataaga tcaaccagat cagaccgtac
     6481 cttgcctttc catctttcaa agagcctggc cgagttttgt gaggactggt cgaaccgtcc
     6541 ttcacttttg gattccaggt gaattatttc acattgcggc tggtaaacaa tcttctttcc
     6601 tttatccact atcctcaggc acaggtcaat atcttcggag ccattaatga atcctgtgtc
     6661 aaatccacct attgcaagaa agtcttcccg ccgcatgatc atacatgctg ccgtaacaca
     6721 ttgcacttct ttgccaatat tggcaggtgg gaaatcagac ggaaatccgt tgtaaacgtg
     6781 accgaagctc actcccgtca attcaggcaa ataaacaccg gcatgctgaa tcatactatt
     6841 cggatagagc agttttgccc caaccatcca ggtatccctg tcattttcaa tcgtatcaag
     6901 gagaatttta tcccagttta ttgtcacaag ggtatcatta ttcaaaaaga caataaattg
     6961 acccctggcc acctttgcgc cttcattgca tgatcgggcg aatttcaggt tggaagagtt
     7021 cctgtggtaa atcaaccagt tattttcttt ttcaaatccg gt
//
LOCUS       DAOQXS010000079         7025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11108_length_7025_cov_3.345624, whole genome shotgun sequence.
ACCESSION   DAOQXS010000079 DAOQXS010000000
VERSION     DAOQXS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_7025_cov_3.345624"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2338)
                     /gene="bglX"
                     /locus_tag="PKM34_03490"
     CDS             complement(83..2338)
                     /gene="bglX"
                     /locus_tag="PKM34_03490"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNQ82680.1"
                     /translation="MVFLAGCMENVDKAPGDGKMNAFVDELMSKMTLQEKIGQLNLPG
                     SGDIVTGQTSNSDIGLKIKEGKVGGLLNIKSVAKIRDVQKVAVEESRLGIPLIFGMDV
                     IHGYETTFPIPLALAAGFDMALIEQSARVAAVEASADGICWTYSPMVDITRDPRWGRI
                     AEGAGEDPYLGSLAAKAYVKGYQGDDLAKNNTIMACVKHYALYGGSEAGRDYNTVDMS
                     RIRMYNEFFPPYKAAVEAGAGSVMTSFNEVDGIPASGNRWLMTEVLRDQWGFDGFVVT
                     DYTAINEMEAHGMGDLQDVSALALKAGVDFDMVGEGFLTTLEKSLEEGKVTKAEIDLA
                     CRRMLEAKYKLGLFEDPYRYCDFERAKNEISTPEHKRIAREAATQTFVLLKNDGQALP
                     LEKKGKIALIGPLADNRFNMAGMWAVACDHEQSVTVLEGFRNVLGDKAEVLYAKGSNI
                     VDDPEYDKRFNWGQSGVDPRTPEQMRQEAVALAARCDVVVAVMGEGAEMSGESSCRTE
                     IGLPGSQQELLKALLKTGKPVVMVLFTGRPLGLNWEQENIPAILNVWFGGTESGNAIA
                     DVVFGDVNPSGKLPVTFPQNVGQVPLYYNHKNTGRPLGESVWYQKYRSNYMDVTNDPL
                     YPFGYGLSYTTFTYGDLKIDRTELSAKKTVTASIDVTNTGNIDGAEVVQLYIRDKVGS
                     ITRPVKELKGFQKIFLKAGETKTVTFTITPEDLKFYNSDLVYDWEPGEFEIMAGGNSR
                     DVASQLVSWMK"
     assembly_gap    2719..2818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2819..4488)
                     /locus_tag="PKM34_03495"
     CDS             complement(<2819..4488)
                     /locus_tag="PKM34_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNQ82681.1"
                     /translation="MKKLLVILAIFMILPTGISAQQKTYCNPVDIDYGYTPIPNFSQW
                     GKHRATADPVIVNYKGDYFLFSTNQWGYWWSSDMVNWNFVPRLFLRPWNEGYDELCAP
                     AVGIIGDTMLVFGSTYTSEFTIWMSTNPKEDDWKPLVERFTIGGWDPAFFTDDDGRFY
                     MYNGSSNEYPVYGVELDRKTMEPKSMRREMYILEDWRYGWQRFGEHMDNTFLDPFIEG
                     AWMTKHNGKYYFQYGAPGTEFTGYADGVVVGKDPLFRDENIPQSMPFSYKAGGFANGA
                     GHGATFQDNWGSYWHVSTIGVCVKNNFERRIGIWPAGFDKDDVMYCNTAFGDYPHYLP
                     DGPADHLQSRFTGWMVLNYQKPVTVSSTLGSYYPNFAVDENIRTYWSAETADEGEWIQ
                     TDLGEMSTVYAIQVNYADQDAEFLGKSSGVFHQYKILESIDGKKWNVLVDKSKNKTDV
                     PHDYTELPSPVKTRYLKLVNIHMPTGKFAISGFRIFGLGAGEKPGAVKDFMVLRTKKD
                     KRSAWLRWSPVDNAYAYNIYMGVEPDKLYNCLMVMSANEYWLKTMDAQA"
     gene            complement(4657..>7025)
                     /locus_tag="PKM34_03500"
     CDS             complement(4657..>7025)
                     /locus_tag="PKM34_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNQ82682.1"
                     /translation="SAFFRIMVENENIIRVRVSPDIIVNDFSYVLVDQNDAPMDFILT
                     EENNLIRLSTKDIEMEIEKSPSFRVIFRNKEGEVINEDVAGQGFGTSFIGNRSTLYKK
                     LFPDERFVGLGEVPGNLDHRGSAYTLNNTDTYRYGDPRLPMYVSIPFYIGIHDSLAYG
                     LFYHNTHKTFFNFGISTDFASITAEDGDVDYFFIHDESIAKIIGHYTMLTGRMPLPTR
                     WSLGYHQSRCSYYPQSNVKILAETFRAKQIPIDCIVLDADYLHEYEPFRINTERFPDL
                     PGLTRYLKSLNIEVTASVNPGIKRDTTYFAHHDGLKKDVFVKFSDGSLYTADIAPSNN
                     NFVDFTNPKARQWWADNMKFLPDNSIHGYWNDMNEPAVGGSYLPDNLLFDFDGRKANA
                     LEAKNLYGMLMARSSFESALKYGENRRPFVLTRSGFAGVQRYAAVWTGDNTAKDEYLL
                     GGMLLNTQLGLSGLPFVGDDIGGYIGPTSKELFIRWIEVGAFEPFMRNHKEAFALANE
                     PWSYGEEAEAIAREYIGFRYRLMPYLYSKFFQATQTGIPVARSLCIDNPFDPLVYDHL
                     YQYQFLFGDAILVVPVTSSEKNKKFYLPQGKWYDLYSDETFEGNRELARECGIYEIPL
                     FVRGSSVIPMQKPVQSTKDHPGDTLIIHIYNGDEKNIFEYYEDDGSTMDYQKGLFYKR
                     KIIFDPANRELAISTPEGNYKSRFTYIQCVFHGFSEMNELKVNGEAILLNCQTVKLSD
                     GLRYLEDIYDPGYYRSLREREIIQEQKSITIPNFENEITIHFYSGSGK"
BASE COUNT         1470 a         1962 c         1722 g         1771 t
ORIGIN      
        1 cccaaatccc caaatcgtca aatcgcccaa tcgcccaatc gcccaatcgc ccaatcgccc
       61 aatcgtccaa tcgttaaatc ctctacttca tccagctcac caattgcgaa gccacatccc
      121 ttgagttccc gccggccatg atctcgaact cgcccggctc ccagtcgtag accaggtccg
      181 aattatagaa cttcaggtct tccggcgtaa ttgtaaaggt cacggtcttt gtttcacccg
      241 ctttcaggaa tatcttctga aatcctttca gctccttcac cggccgggtg atgctgccga
      301 ccttatcgcg gatataaagc tgtactactt cggcgccgtc tatgttgccg gtattggtca
      361 cgtcgatgct ggccgttacc gtcttcttgg cagaaagctc tgtccggtca atcttcagat
      421 caccgtaggt aaatgtggtg tagctgagcc cgtagccgaa cggatacagc ggatcgttgg
      481 tcacgtccat gtaattcgag cggtatttct ggtaccacac gctctcgccc agcgggcggc
      541 cggtattctt gtgattgtaa taaagcggca cctggccgac attctgcgga aaggtcaccg
      601 gcagcttccc cgaagggttc acatcgccga aaaccacgtc ggcaatggcg ttgccacttt
      661 ccgtacctcc aaaccagaca ttcaggatgg ccgggatgtt ctcctgttcc cagttgagcc
      721 ccagcggacg gccggtgaac aggaccatca cgaccggctt tcccgttttc agcaatgctt
      781 tcagcagttc ctgctggctt cccggcaggc cgatctctgt ccggcacgag ctctctccgc
      841 tcatttccgc accctcgccc ataacggcca ccaccacatc gcagcgggca gccagggcaa
      901 cggcctcctg ccgcatctgt tccggggtgc ggggatcaac gcccgactgg ccccagttga
      961 accgcttgtc atactccgga tcgtcgacga tattgctccc tttggcataa aggacctcgg
     1021 ccttgtcgcc cagcacattc cggaaaccct ccagcaccgt caccgattgc tcatggtcgc
     1081 aggctacggc ccacatcccc gccatattga agcggttgtc ggccagcgga ccgatcagtg
     1141 cgatctttcc ctttttctcc aggggcaggg cctggccgtc atttttaagc agaacgaacg
     1201 tttgggttgc ggcttcccgg gcaatccgtt tgtgttccgg ggtggagatc tcattcttag
     1261 cccgttcgaa atcgcagtac cggtagggat cttcgaataa tcccagcttg tatttggctt
     1321 ccagcatgcg tcggcacgcc aggtcgatct ccgcctttgt cactttccct tcctccagcg
     1381 atttctccaa tgtcgtcagg aacccttcgc cgaccatgtc gaaatcgaca ccggctttca
     1441 gtgcaagcgc cgaaacatcc tgcaaatctc ccattccgtg tgcttccatc tcattaattg
     1501 ctgtgtagtc ggtcacaaca aatccgtcaa agccccactg gtcgcgcaat acttcagtca
     1561 tgagccagcg gttgcccgat gccgggatgc cgtcgacttc gttgaaagag gtcatcacgc
     1621 tgcctgcgcc cgcctccaca gcggctttat aaggcgggaa aaactcgttg tacatcctga
     1681 tccggctcat atcgacggtg ttgtaatccc ttccggcttc agagccgccg taaagcgcat
     1741 aatgcttcac gcacgccatg atcgtgttgt ttttcgccag gtcgtcgccc tggtaacctt
     1801 taacgtaagc ttttgccgcc agcgagccca ggtaaggatc ttcgcccgct ccttcggcga
     1861 tgcgccccca gcggggatcg cgcgtgatgt caaccatcgg cgagtaagtc cagcagatac
     1921 cgtcggcact ggcctcaacg gcagccaccc gcgcactctg ctcgataagt gccatatcga
     1981 aacccgccgc cagcgctaac gggataggga aggtcgtctc atagccatgg atcacatcca
     2041 tcccgaaaat gagcggaatg cccagccggc tctcttccac cgccactttc tgcacatcgc
     2101 ggatcttggc caccgacttg atattcagca atccgccgac ttttccttct tttatcttga
     2161 gaccaatatc ggaattcgat gtctgcccag tcacaatatc cccgcttccc ggcaggttca
     2221 gctgcccgat cttctcctgc agcgtcatct tcgacatcag ctcatcgaca aaggcattca
     2281 tcttcccgtc tcccggcgct ttatcaacat tctccataca ccccgccagg aaaaccatcc
     2341 ccagcagcaa agcaaaaaat ataagtcttt gtttcatggt tctattgttc tattgttcta
     2401 ttgttctatt gttccattgt tccattgttc tattgttcta ttgttccatt gttataagat
     2461 tcgaaaactc acaagctcac aagttcacaa actcacaaac tcacaagctc acaagttcac
     2521 aaactcacaa gctcacaagt tcacaaaatc acaagctcac aagttcataa actcacaata
     2581 tcataaatca ctccaccctc atcacctcgc ttcgccgccc cactccattc tcattcaccg
     2641 cttctatcgc gaaatagtat gtcgcttgcg catccattgt cttcagccag tattcgttgg
     2701 cgctcatcac catcaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     2821 ttgcgcatcc attgtcttca gccagtattc gttggcgctc atcaccatca ggcagttata
     2881 aagcttgtca ggctccacgc ccatgtagat gttgtacgca taggcattat ccaccgggct
     2941 ccatcgcagc cacgcgctgc gcttgtcctt cttggtccgg agcaccatga agtcctttac
     3001 cgctcccggc ttctctcccg cgccgaggcc aaaaatacga aatccgctga ttgcaaactt
     3061 gcctgtcggc atatgtatgt tgacgagttt caggtatcgg gttttgacag gtgaggggag
     3121 ctcggtgtaa tcgtgcggca catccgtttt gtttttgctt ttatccacca gcacgttcca
     3181 tttcttccca tcaatggatt caaggatctt atactgatga aaaacgcccg acgacttgcc
     3241 gaggaattcc gcatcctggt cggcgtaatt gacctggatg gcatacacgg tcgacatttc
     3301 gccaaggtcg gtttggatcc attccccttc atcggccgtt tcagccgacc agtaggtcct
     3361 gatgttctca tcgacagcaa agttgggata gtaagagcct aaagtggagg atacggtgac
     3421 cggtttctgg taattcagca ccatccagcc ggtgaagcgc gactgcaggt ggtcggctgg
     3481 accgtcgggc aggtagtgcg ggtaatcgcc gaaggctgtg ttgcagtaca tcacatcatc
     3541 cttgtcgaag ccggcgggcc agatgccgat cctccgttca aaattgttct tcacgcaaac
     3601 gccgatcgtg gagacgtgcc agtagctgcc ccagttgtcc tggaaagtgg cgccatggcc
     3661 ggcgccgttg gcaaaaccac ccgccttgta gctgaatggc atcgactgcg gaatattctc
     3721 atccctgaaa aggggatctt tccctaccac cacgccgtcg gcatagcccg taaattcggt
     3781 ccccggcgcc ccgtactgga agtagtattt gccgttgtgc tttgtcatcc aggccccttc
     3841 gatgaacgga tcgaggaagg tgttgtccat atgctcgccg aatcgctgcc agccgtagcg
     3901 ccagtcttcc aggatataca tctcccggcg catcgacttc ggctccatcg ttttccggtc
     3961 gagctccaca ccgtagaccg ggtattcgtt gctgctgccg ttgtacatgt agaaccggcc
     4021 gtcgtcgtcg gtgaaaaatg ccgggtccca gccgcctatc gtgaaccgtt ccaccagcgg
     4081 cttccagtcg tcctccttcg ggttggtgct catccagatg gtgaactcgc tggtatacgt
     4141 cgatccgaag accagcatcg tgtcgccgat gatgccgacg gcaggtgcgc acagctcgtc
     4201 gtagccctcg ttccaggggc ggaggaacag gcgcggcacg aaattccagt tcaccatgtc
     4261 gctgctccac cagtagcccc actggttggt ggagaacagg aaatagtcac ctttgtaatt
     4321 cacgatcacc gggtcggccg tggcgcggtg ctttccccat tggctgaagt tcgggatagg
     4381 ggtgtagccg taatcgatat cgaccgggtt gcagtaggtt ttctgctggg cggaaatgcc
     4441 agttggcaat atcatgaaaa ttgccagtat tactagtaat tttttcatac tttcattaat
     4501 tgatctgctg agtcccttcc gacgactgaa gccgtcggtt gctatctttt tcttccgatg
     4561 actaaagccg tcggttgcta tctttttctt ccgacgacta aagccgtcgg ttgctatctt
     4621 tttcttccga cgactaaagc cgtcggttcc aagggattat ttacctgagc ctgaatagaa
     4681 atgaatcgta atctcgtttt caaaattcgg gattgtaata ctcttctgtt cctgaattat
     4741 ttccctctct ctcagactcc tgtaataccc cggatcataa atatcttcca aatacctcaa
     4801 cccatctgac aatttaacag tttgacaatt taacaatatc gcttccccgt tgactttcaa
     4861 ttcattcatc tccgaaaaac catgaaagac acattgaatg tatgtaaaac gcgatttata
     4921 atttccttcc ggtgtggaga ttgccagttc acggtttgca ggatcaaaga tgatttttct
     4981 tttataaaac aggcctttct gataatccat ggtgcttccg tcatcttcat aatattcaaa
     5041 gatgtttttt tcatcgccgt tgtaaatatg gatgataagc gtatctcccg gatgatcttt
     5101 ggtggattgc accggttttt gcatggggat gacagaggaa cccctgacga acagagggat
     5161 ctcatagatt ccgcattccc ttgccagttc ccggtttcct tcaaatgttt catccgaata
     5221 aaggtcatac catttccctt gcgggagata gaacttttta tttttttccg atgatgtcac
     5281 cggaacgacc aggatggcgt cgccgaacag gaactggtac tggtaaagat gatcataaac
     5341 cagcgggtcg aaagggttat cgatacagag gctgcgggca acggggatcc cggtttgtgt
     5401 tgcctggaag aatttagaat agagataagg catcagccgg taacggaagc cgatgtattc
     5461 cctggcgatc gcctcggctt cttcgccgta gctccagggc tcgttggcaa gggcaaaggc
     5521 ctccttgtgg ttgcgcataa agggttcgaa agcaccgacc tcgatccacc ggatgaacag
     5581 ctcttttgac gtcggcccga tgtatccgcc gatgtcgtcg cccacaaatg gcaatcccga
     5641 caggccgagc tgggtattta gcagcattcc gccaaggagg tattcgtctt tcgccgtgtt
     5701 atcgcctgtc cacaccgcag catagcgctg cacacccgca aagcccgagc gggtcaggac
     5761 gaacgggcgc cggttctccc cgtatttcag ggccgattcg aagctgctgc gggccatcag
     5821 catcccgtat aggttcttgg cttccagggc attggctttg cgcccgtcga aatcgaagag
     5881 caggttatcg ggcaggtagc tgccgccgac ggccggttcg ttcatatcgt tccagtagcc
     5941 gtggatgctg ttgtcgggca ggaatttcat gttgtcggcc caccattgcc gggctttggg
     6001 gttggtgaag tcgacgaagt tgttgttgct cggggcgata tcggccgtgt agaggctgcc
     6061 gtcggagaac ttcacgaaga catccttttt cagcccgtcg tggtgggcga agtaagtcgt
     6121 gtcgcgtttg atccccgggt tgaccgaggc ggtgacctcg atattcagcg atttcaggta
     6181 acgtgttaat cccggcagat cgggaaagcg ctctgtattg atccggaacg gctcgtattc
     6241 gtgcaggtag tcggcgtcga gcacgatgca gtcgatgggt atctgctttg cgcggaaggt
     6301 ttctgccagg atcttcacgt tgctttgcgg gtagtagctg caccggctct ggtggtagcc
     6361 gagactccat cttgtgggca gcggcatccg gccggtaagc atggtataat ggccaatgat
     6421 ctttgcaatg ctctcgtcat ggatgaagaa ataatcgaca tcgccgtctt cggcagtgat
     6481 ggaggcgaaa tccgtagaga tcccgaagtt aaaaaacgtt ttgtgggtgt tatggtagaa
     6541 cagcccgtat gccaggctgt cgtggatgcc gatatagaac gggatgctga cgtacatcgg
     6601 caggcgcggg tcgccgtagc ggtaggtgtc ggtgttgtta agcgtatagg cgcttccgcg
     6661 atggtcgagg ttgccgggga cttcgcccag cccgacgaag cgctcatccg ggaatagttt
     6721 cttataaagc gtggagcggt tgccaatgaa gctcgtcccg aatccctggc ctgccacgtc
     6781 ttcgttgatg acttctcctt ctttattcct gaaaatgacc cggaatgagg gtgatttctc
     6841 aatctccatt tcgatgtcct tggtagaaag tctgatcagg ttgttttctt ccgttaaaat
     6901 gaaatccata ggagcgtcgt tctgatcgac aagcacgtaa gagaaatcat tcacaattat
     6961 atcaggagag acccttaccc ggatgatatt ttcgttttca accatgatcc tgaagaaggc
     7021 ggatg
//
LOCUS       DAOQXS010000080         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11201_length_6978_cov_3.663007, whole genome shotgun sequence.
ACCESSION   DAOQXS010000080 DAOQXS010000000
VERSION     DAOQXS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11201_length_6978_cov_3.663007"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="lpxD"
                     /locus_tag="PKM34_03505"
     CDS             <1..681
                     /gene="lpxD"
                     /locus_tag="PKM34_03505"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNQ82683.1"
                     /translation="DVYIGEFTFIGENVVIENNTKIFPHVFLGDNVKVGSHTTLFAGV
                     IVYSDNSIGSNCTIHGGVVIGADGFGFAPQQDKNYKKVAQIGNVVIEDNVEIGSNTTI
                     DRATLGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQTGISGSTKIGKNCMIAGQVGF
                     VGHLIIGDDVKIGAQSGVSGNLKEGSISMGSPAIDIAVHRRALVHFKNLPGIVKRIDE
                     LEKRLNKE"
     gene            726..2147
                     /locus_tag="PKM34_03510"
     CDS             726..2147
                     /locus_tag="PKM34_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNQ82684.1"
                     /translation="MTENQKTIKRPVSLAGVGLHTGQEVNLTFRPAPINYGRRFVRVD
                     LDGRPEIEAVVENVVDTARGTSIASNGAKVQTVEHVLAAVAGSGIDNIIIEVDQAETP
                     IMDGSSRFFVEALDQCGIQEQPALKEYLELRTPVFYEEPENKIEMVAMPYPRFKVTTM
                     INFETKVLGAQYAIMENMEDFRREISMCRTFVFLHELEFLLKNELIRGGDLNNAIVFV
                     NRAVSQDELNRLAKLFNKPSVEVLNEGILNNLELHFENEPARHKLLDVVGDLSLLGKP
                     IKAHIIARRPGHTANAKFAQLIRQQLLKDKKMTQAPLYDPNKPPILDINQIKNILPHR
                     PPFLLIDKIIEMTETRVVGVKNVTMNEQFFEGHFPDEPVMPGVLQVEAMAQAGGVLVL
                     STVPNPQDYTTLFLKIDQVKFRQKVVPGDTLIFDLKLAGPIRRGIAHMEGVAYVGNKV
                     VMEAVMMASIVEKGTNNIANPKK"
     gene            2144..2944
                     /gene="lpxA"
                     /locus_tag="PKM34_03515"
     CDS             2144..2944
                     /gene="lpxA"
                     /locus_tag="PKM34_03515"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNQ82685.1"
                     /translation="MIQPLAYVHPQAKVANNVIIDPFVTIEKNVEIGEGSWIGHHATI
                     MEGARIGKNCRIFPGAVISAIPQDLKFDGEDTTVHIGDNTTIREFVTVNRGTKASYKT
                     VIGNNCLLMAYVHVAHDCIIGNHVILANAATLAGHIEIDDFAIVGGLAAVHQFVKIGA
                     HSMISGGGMARKDIPPYTKAAREPLSYVGVNSIGLRRRGFTNEQISQVQEIYRIIYRK
                     NYNVSQAVSIIEADVPATPERDEILTFISRSTRGIMKGYTSLLGNNRE"
     gene            complement(2996..3991)
                     /locus_tag="PKM34_03520"
     CDS             complement(2996..3991)
                     /locus_tag="PKM34_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNQ82686.1"
                     /translation="MLSVVIIAIVIALLFDFLNGMNDAANSIATVVSTKVLPPLVAVV
                     WAAFFNFAAVFFFGVHVATTIGKGIVEPAIINEFVILAALVGSVVWVYVCTNLGLPIS
                     VSQALIGGLIGPALVIGGKSVLVSSGIIKVALFIVLSPLIGLILGYFLMILTMLVFHW
                     TSPRKVDHLFRGLQLVSSAIFSIGHGTNDAQKTMGIISVLLFSTGRLGPEFHVPTWVI
                     IASYSAISLGTLAGGWKVIKTLGLNLTALKPVHGFCAETAGAITIIGSSLAGIPVSTT
                     HTITGSIMGVGMTRRLSAVRWGVVGKIVMAWVLTIPGTIILSAALYYIISLFLNN"
     gene            complement(4002..4649)
                     /locus_tag="PKM34_03525"
     CDS             complement(4002..4649)
                     /locus_tag="PKM34_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNQ82687.1"
                     /translation="MSINTFFQKLVPKDKKFFPMFESQADLIVVAAGKLNEIFITNDP
                     ERHAVLIREIKDLENQGDEVAHQIFDELDKTFITPFDREDIHQLTSTLDDVLDFINGS
                     SQRIRLYRLKSFPAEFVRFSSVLLSGANEIRSAVRELYNLKRPEMIRQACIRVNEVEN
                     QADDLYHFVISDLFENEKDAIELIKKKEVLQTMERASDRMEDVADVLKTIIIKLA"
     gene            complement(4694..6145)
                     /locus_tag="PKM34_03530"
     CDS             complement(4694..6145)
                     /locus_tag="PKM34_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ82688.1"
                     /translation="MKRLNFRLLVYLAGLILLIESGFMMACIPVSLIFSGGDFRALAL
                     SAGITGLFGAILFFYYRKRVRKTPGIREGFLAVTMTWLLISVFGALPYMIHGSIPNPV
                     DAWFETVSGFTTTGASILTDIEAVPKGLLLWRSLTHWMGGMGIILMVIVVLPSLKVGG
                     VYLFSAEASKISLDDIKPRVVDTAKRFGIIYIALTLIEIVLLTLGGMPVFDSVCHSFA
                     TIATGGFGTKNTSITNYSVYIQYVIIAFMFLSGINFTLHFLVSKGEIKKALRNEELRF
                     YTQVTLAITLIIFLILYFNGTSLHKAFRDSLFQVVSIITCTGFATADYLLWPAAGWFM
                     IFLTMFVGASAGSTGGGIKVIRHLLMVRNLRMGLKTFLHPRVIYGVKYQGNLVSEELL
                     RSVIAFYIWYLIIFFFSSLLMMIIGVDFLTSIGGVATSMGGIGPGLGTVGPASNFHHL
                     PSLAKILLTFDMIIGRLEIYAFLVLFSPAFWKR"
     gene            complement(6210..>6978)
                     /locus_tag="PKM34_03535"
     CDS             complement(6210..>6978)
                     /locus_tag="PKM34_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82689.1"
                     /translation="SVVGDTTTTLMWIDGVEPVWVLHAYVASFAALIIFGIFGSIKQH
                     KFQPIVKDNNPGIKIDWTKIFIVALILVGAIATNVLFDFPALGVWIAILLGALISKTP
                     WFELKHAWQGTIFLMSLVMIASMMPVEELPPASWQTAFILGFVSAVFDNIPLTKLCLE
                     QGGYDWGVLAYTVGFGGSMIWFGSSAGVALSNMYPQAKNTVNYIKHSWFVIVAYVVGF
                     FVLLWVIGWEPHAPHKKGADSGNPDLKPVPEQSIHDH"
BASE COUNT         2022 a         1665 c         1646 g         1645 t
ORIGIN      
        1 gatgtttata tcggtgaatt tacctttata ggcgaaaatg ttgtgatcga aaacaatacg
       61 aagattttcc cccatgtttt cctgggtgat aatgtgaaag tcggaagcca tacgactttg
      121 tttgccggcg ttattgtata ttccgacaac tccatcggaa gcaactgcac catccatggc
      181 ggtgtggtta tcggggcaga cgggttcggg tttgcaccgc agcaggataa gaattacaag
      241 aaagtagctc agatcggcaa tgtcgtcatt gaagacaatg ttgaaattgg ctccaacacc
      301 acgatagacc gggctacatt gggatcgacc attatccgga aaggggccaa actcgacaat
      361 cttatccaga ttgcgcacaa tgtagagatt ggcgagaata cggttattgc tgcacaaaca
      421 ggcatatccg gatcaacaaa gatcggtaag aactgtatga ttgcggggca ggtcggattt
      481 gtggggcacc tcattatcgg cgacgatgta aagatcggag ctcaatcagg cgtttcgggc
      541 aacctgaaag aaggctctat cagcatgggt tccccggcca tagatattgc cgttcatcgc
      601 cgcgccctcg tccatttcaa aaatcttccc gggatcgtta aaagaatcga cgaactcgaa
      661 aagcggctta acaaggaatg atccggatcg cgaacgggaa gaaatgttaa ttttaccggc
      721 tagttatgac agaaaatcaa aaaacaataa aaagaccggt cagccttgcc ggagtcggat
      781 tgcatacggg ccaggaggtg aatctgactt tcaggccagc cccaattaat tacggaagga
      841 gatttgtccg ggtagatttg gatggccggc ctgagattga agccgttgtt gaaaatgtgg
      901 tggatacggc ccggggcacc agcattgcga gcaacggggc caaggtgcag accgtcgaac
      961 atgtactggc tgccgtagcc ggatcgggta tcgacaatat catcatcgaa gtagaccagg
     1021 ctgagacccc cattatggac ggcagttcca ggttttttgt ggaagcgctc gaccagtgcg
     1081 gcatccagga acagcccgct ttaaaggaat atcttgagct caggacgcct gttttttatg
     1141 aagagcctga gaataagatc gagatggtgg ctatgcccta ccccaggttc aaagtcacta
     1201 cgatgatcaa ttttgagacc aaggtcctgg gcgcacaata cgcgatcatg gaaaatatgg
     1261 aggatttccg cagggaaatc tccatgtgca ggacttttgt ttttttacat gagcttgaat
     1321 ttttgctgaa aaacgagctg atccgcgggg gtgatctcaa taacgccatt gtatttgtaa
     1381 acagggcggt gagccaggat gagctcaaca ggctggccaa attgttcaat aaaccttcgg
     1441 tagaggtatt gaatgaaggt attttaaaca accttgaact ccattttgag aatgaaccgg
     1501 cccggcataa attactggat gtggtcggcg atctgtcgtt gctcggcaaa ccgatcaaag
     1561 cccatatcat agcccgcagg ccgggacata cggccaatgc gaaatttgcg caactcatca
     1621 ggcaacaact cttaaaagat aaaaagatga cacaggcacc gttgtacgat ccaaacaagc
     1681 cgccgattct tgacatcaac cagatcaaga atatattacc gcaccggccg ccgtttctgc
     1741 tgattgataa aatcattgaa atgactgaga cacgtgttgt cggggtgaaa aatgttacta
     1801 tgaatgaaca attttttgaa ggtcattttc cggacgagcc ggtcatgccg ggtgttctgc
     1861 aggtcgaagc catggcccag gccggcggtg tgctggtttt aagcacggta cccaatcccc
     1921 aggactatac gacccttttc ctgaaaatcg accaggttaa attccgtcag aaggtcgttc
     1981 cgggcgatac ccttattttc gacctgaaac tggccggccc gatcagaaga ggcattgccc
     2041 atatggaagg tgttgcttat gtcggcaata aagtggtgat ggaagcagtt atgatggctt
     2101 ccatcgtaga aaaaggaact aataatatcg caaacccaaa gaaatgatcc agccgttagc
     2161 ttacgttcat ccgcaggcaa aagtcgccaa taatgtcatt atcgaccctt tcgtcactat
     2221 tgaaaaaaat gtggagatcg gggaaggttc ctggatcggg caccatgcaa ccattatgga
     2281 aggggcccgg atcggtaaaa attgccggat ttttcccgga gccgttattt cagccattcc
     2341 acaggatctg aaattcgacg gggaagatac cacggtccat attggtgaca atacgaccat
     2401 ccgcgaattt gttaccgtta accgggggac aaaagccagt tataaaacgg tgatcggcaa
     2461 caactgcctg ctgatggcct atgtgcatgt cgctcacgac tgcattatcg gaaaccatgt
     2521 gatcctggcc aacgctgcca cgctcgccgg tcacattgag atagacgact ttgcgattgt
     2581 cggcggcctg gcggctgttc accagtttgt caagatcggc gcccactcca tgatctcggg
     2641 cggcggtatg gcccgtaaag acatacctcc atatacgaaa gcagctcgtg aacccctctc
     2701 ctatgtcggg gttaactcca tcggactgcg ccggagaggt ttcaccaatg aacagatcag
     2761 ccaggtacag gaaatctacc ggatcatcta ccggaaaaat tacaatgtat cacaggccgt
     2821 tagcatcatt gaagccgatg tcccggcaac accggaaaga gatgagatcc tgacgtttat
     2881 ctcacgttca acccggggta tcatgaaagg atataccagc ctgctgggca acaacaggga
     2941 gtgatttgaa tcagtcgcca ggcgttggtc gttattcgtt gtttgatggt cctttttaat
     3001 tattcaagaa taatgaaata atatagtaca gtgcggcgct cagaatgatt gttccaggaa
     3061 tggtcagtac ccacgccata acgatcttac cgacaacgcc ccacctgact gcggaaagcc
     3121 tgcgggtcat tccgactccc ataattgaac cggtaatcgt atgagtggta cttacaggta
     3181 tcccggcaag agaactgcca ataatggtta tggcaccagc cgtctcggcg cagaatccat
     3241 gtacaggttt cagagcggtc aggtttaatc cgagcgtttt gattactttc catccaccgg
     3301 ccagggtgcc aagcgaaatc gccgaatatg acgctattat aacccatgtc ggcacatgaa
     3361 actccgggcc cagccggccc gtgctgaaca gcagcactga aatgataccc attgttttct
     3421 gtgcatcatt ggtaccgtgt ccgatactga aaatcgccga agaaaccagc tgcaaacccc
     3481 tgaacaggtg atccactttc ctcggggagg tccagtgaaa aaccagcatt gtcaggatca
     3541 tcaggaaata gcccaaaatc aggccgataa ggggtgataa aacgatgaac aaggcaactt
     3601 taatgatacc ggaagagacc aggacagatt tcccgccgat gacaagagcc gggcctatta
     3661 atccgccgat cagggcttga gatacactga tcggcaggcc caggttagtg catacataca
     3721 cccagacaac ggatccgacc agtgcggcaa ggataacaaa ctcattgatg atggccggtt
     3781 cgacgattcc tttgccaatc gtggtggcca catgcacacc aaagaaaaac actgcggcaa
     3841 agttgaaaaa agccgcccat acaactgcca ccagcggggg tagcactttt gtcgaaacaa
     3901 ctgtcgcaat agaattggct gcatcattca tcccgtttag gaaatcgaag agcagggcga
     3961 tgacaatggc aataatgaca actgaaagca tgtgatccgg tttaagccag tttaattatg
     4021 atggttttta aaacgtccgc aacgtcttcc atccggtcgc tggcccgctc catcgtttgc
     4081 agaacctcct tcttcttgat cagttcgata gcatccttct cattctcaaa aagatctgaa
     4141 atcacaaagt gataaaggtc atccgcctgg ttttccactt catttacccg gatacaggcc
     4201 tgcctgatca tctcgggccg tttaagatta tacaattcac ggaccgcgct tcggatctcg
     4261 ttggctccac ttaataaaac cgaagaaaac cgcacaaatt cggcgggaaa tgattttaac
     4321 cggtataacc tgattctctg gctggagcca ttgataaagt cgaggacatc atcgagggtc
     4381 gaggtaagct ggtgtatgtc ttccctgtcg aaaggcgtga taaaagtttt atcaagctca
     4441 tcaaagatct ggtgagccac ttcatccccc tgattttcca gatcttttat ttcacggatc
     4501 aatacagcat gtctttcagg atcatttgtg ataaatattt cgttgagttt tcctgcagcc
     4561 acaacaatta aatcggcctg ggattcaaac atgggaaaga acttcttatc ctttggtacc
     4621 agtttctgaa aaaatgtatt gatactcata ttcttggttt ttagtttctt tgagggtttt
     4681 ctttaaataa atatcaccgt ttccagaagg ccggtgaaaa tagaacaaga aaagcatata
     4741 tctccaaccg gccaataatc atgtcaaatg taagtaaaat cttagctaaa gaaggaaggt
     4801 ggtgaaaatt actcgccggg cccacagtac ccaaacccgg gcctatgccg cccatgctgg
     4861 ttgccactcc accgatcgat gtaagaaaat caaccccgat gatcatcatc agtaatgaac
     4921 tgaaaaagaa aatgatcagg taccaaatgt aaaaagcgat gaccgatctc aacagctcct
     4981 ctgatacaag attcccctgg tattttacgc cataaataac ccttgggtgc agaaatgtct
     5041 taagacccat tctcagattc ctaaccatca gaaggtgccg gatgaccttg atccctcccc
     5101 ctgtagatcc ggcagaggcc ccgacaaaca tggtcaggaa gatcataaac cagccggctg
     5161 ccggccagag cagatagtcg gccgtagcaa atcctgtaca ggtgatgatc gacaccacct
     5221 ggaacaagga atcccggaag gctttatgaa gagaggttcc attgaaataa agaatcaaaa
     5281 aaattatcag tgtgatggcc agagtgacct gagtataaaa cctcaattct tcatttctca
     5341 atgctttctt tatctcccct ttcgacacta aaaagtgtag agtgaaatta attcccgaaa
     5401 ggaacatgaa agcgataata acatattgaa tataaacaga atagtttgta atactggtgt
     5461 tctttgtccc gaatcctccc gtggcgatcg tcgcaaatga atggcagaca ctgtcgaaaa
     5521 ccggcatacc tcccaaggtc agcaaaacaa tttcgattaa tgtaagagca atataaataa
     5581 taccgaatct tttggcagta tctactaccc tgggtttgat atcgtccagg ctgatcttgg
     5641 aagcttcggc gctgaaaaga taaactcccc cgactttcag ggaaggcagt accacgataa
     5701 ccatcaggat gatccccatc cctcccatcc agtgggtcag gcttctccag agaagcaaac
     5761 ctttgggcac cgcctcgata tcggtgagaa tagaggcgcc ggtagtggta aaaccggaaa
     5821 cagtctcaaa ccaggcatcc accggatttg gaatcgaacc atgaatcata taaggaagtg
     5881 caccgaatac ggagatcaga agccaggtca tggtgacagc aaggaatcct tccctgatcc
     5941 cgggagtctt tcttaccctc ttccggtaat aaaagaataa aatcgcacca aataatcctg
     6001 taatcccggc cgataaagcc agtgctctga agtccccacc ggaaaaaatc agggaaaccg
     6061 gaatgcaagc catcataaag ccgctttcaa tcagcaggat caggccggcc aaatagacca
     6121 gcaaccggaa attcagcctt ttcatcggtc agacgacccg ttattctgag aataggcgtt
     6181 aaaaagcttt tataaaaaca acgttctttt tagtgatcat gaatgctttg ttccggaaca
     6241 ggcttaagat ccggatttcc cgagtctgcg ccttttttat gcggagcatg gggttcccaa
     6301 ccgatgaccc atagtaagac aaagaatcca acgacatagg caacgatcac aaaccagcta
     6361 tgtttgatat aatttactgt attcttggct tgagggtaca tattcgacag ggcaacgccg
     6421 gcggaagaac caaaccagat catcgaaccg ccgaatccaa cggtataagc cagaacaccc
     6481 cagtcgtacc cgccttgctc caggcagagt tttgtcagcg ggatgttatc aaacacggcg
     6541 gagacaaagc ccaggatgaa ggcggtttgc caggaagcgg gaggcagttc ctcgacaggc
     6601 atcatggaag cgatcattac cagcgacatc agaaagatgg tgccctgcca ggcatgttta
     6661 agctcaaacc agggtgtttt gctaatcaat gcccctaaca gaatagcaat ccagacgccc
     6721 agggcaggaa aatcaaataa cacgttcgta gcaatggctc cgaccagaat aagtgcaaca
     6781 ataaaaattt tcgtccagtc gatcttgatt ccgggattat tatcttttac aataggttga
     6841 aatttgtgct gttttattga gccaaaaatg ccaaatatta taagtgctgc aaacgatgcc
     6901 acatatgcat gtaataccca tactggttcc acaccgtcga tccacatcaa ggtcgtcgtc
     6961 gtgtcgccga caacactt
//
LOCUS       DAOQXS010000081         6897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11371_length_6897_cov_3.604063, whole genome shotgun sequence.
ACCESSION   DAOQXS010000081 DAOQXS010000000
VERSION     DAOQXS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_6897_cov_3.604063"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKM34_03540"
     CDS             <1..744
                     /locus_tag="PKM34_03540"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ82690.1"
                     /translation="VRIKATKAEPAGIRLNKYIANAGICSRREADDLIRAGVIKVNGE
                     VVTTLGAKVMPGDKVQYADQTLSNERKQYLLLNKQKGYVTTTKDPHAKNTVMELIAGA
                     CKERIYPVGRLDRNTTGLLLFTNDGELAKKLMHPASRILKMYHVVLDQPLSKQDLMKV
                     AGGIEIDGDRIQVDGIEYVEDAKDRREVGMEIHSGQNRIVRRIFESLGYKVTKLDRVM
                     YAGLTKKNLPRGKWRFLSEQEVINLKKMR"
     gene            757..1248
                     /locus_tag="PKM34_03545"
     CDS             757..1248
                     /locus_tag="PKM34_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNQ82691.1"
                     /translation="MRGDPKLYRILEELGITFDYYEHPAAPTIEEALKNWKNIDSTHC
                     KNIFFRNHKGNRHYLVIMEHSRPLAIHDLEKKLKQGKLTFASPERLMKYLGVTPGSVS
                     LFGLINDEADHVYVFLDEVLREAKNLSFHPNDNTASLVITGEGFHKFLEWSGNGFEFL
                     ALA"
     gene            complement(1346..3460)
                     /locus_tag="PKM34_03550"
     CDS             complement(1346..3460)
                     /locus_tag="PKM34_03550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNQ82692.1"
                     /translation="MRTLFGISLLLMGIITSCTTGEKKMKTAENPFFAPYGTPFETAA
                     FDRIKNEHFLPAFHEGIRVHDEEIAAIVANPAAPDFENTIVALDRSGDLLAGVSGVFY
                     NLTSANTNDELQSIAKEISPVLTGHYDNILLNAGLFKRIKAVYEQRDNLNLDTEQAML
                     LEKTYKQFARGGSNLPDDKQATLREINKELSMLSLQFGDNLLAETNAFRLVIDNESDL
                     EGLPAFVRDAAASAAREDSLDGKWLFTLHSPSRIPFLQFSAKRELREKVLKAYANRGN
                     NNNQYDNKEIAAKMASLRVKKANLLGYATHADYILEENMAKNPAAVYDFLDKVWSAAL
                     PVAKKEAAELQAMIDAEGGNFKLEAWDWWYYAEKLRKQKYDLDEEALKPYFSLENVRL
                     GMFDVATRLFGITFTERNDIQKYHPEVKVFEVKDTDGSHIGILYMDFFPRASKEGGAW
                     MNNYRDQYMAGDSNVSPVITTVCNFTKPAGDVPSLLTFEEVETLFHEFGHALHGLLSE
                     CRYRKLSGTSTPRDFVELPSQIMENWAGDPEVMKLYARHYQTGEVIPQELIDKITASG
                     HFNQGFATVEYMAACYLDMDIHTLKDSSLMDALGFESASMSKIGMMPEIIVRYRVPYF
                     AHIFAGGYSSGYYSYLWAEVLDADAFQAFKETGIFDPATAKAFRDNILSRGGTDDPMK
                     LYKQFRGKEPDVAPMLKRKGLI"
     gene            complement(3529..3783)
                     /locus_tag="PKM34_03555"
     CDS             complement(3529..3783)
                     /locus_tag="PKM34_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82693.1"
                     /translation="MKLYDVISYLGYLSGIFGGLMMLAGVIGFLTGTEFLGVKYYYNY
                     FFYANSFLLFGVFIILGTRQFCHCHCHTHDDACQHEDHQK"
     gene            3897..4475
                     /locus_tag="PKM34_03560"
     CDS             3897..4475
                     /locus_tag="PKM34_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ82694.1"
                     /translation="MTKDQTRDKILAVAAKMFGKYGFQKTTVDEIARTAHKAKGSVYY
                     YYKSKEDLFLAVVMQEIQFLKTGLTRVIVDSQDATGMICNYLMNRMMLMKDAVNYHES
                     LKADFVADFSFLNDCREDFTRFEIDLMKAIIDRGIRENRFQIKDSYATAQVIILAMKA
                     IEIPFYHHHKISEYEQTIVELVDILVRGLEKP"
     gene            4727..>6897
                     /locus_tag="PKM34_03565"
     CDS             4727..>6897
                     /locus_tag="PKM34_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ82695.1"
                     /translation="MKTIAYSIIRYRKAIIILTLIITGIMAYFFRYLKVDPDVFNYLP
                     DDDPKAMLFREIGNKYGGNYTGIIGLETKNIFNTETLENIRRITDSVKIIPGVATVTS
                     LTNIIDIKGSDWGIEIGNLVDKYDIPDTQEELAALRTYTLSQEMYRGAIVSEDATVTA
                     VMVKIAEGVDKIKVASAIREKVESLNLSEKVYFGGMPFAFKSLADIIMGDIVILAPIT
                     ALVIILVLLLSFRSWRGVVMPLVTVVISVIWTIGLMGLFRVEISIISDVTPVILLAVG
                     SAYTIHVINRIRQEKAENPERPMQEALAYIIIPVFLAAATTMAGFISFIFGSYLTMIS
                     TFGIFTALGVFFALILSLTFAPALLYAFPEKKPALPGKTSDRSDLIGMILAKIQRRVI
                     RHPKRVILTWGIILAGAIAGIFMIERNVNILDYFRKSDPTHQSEILFKNKFGGSMPVY
                     ITVKGDVQDPEMLRIMADIGDYLKNDGYVSHAQSVADLIEEMNEVMGEGKMIPDEKAK
                     VEQLWFLLEGQDIMEQLVTYEKDEGLVNTTTGKGDLGEMTGLTENLQAYIERHPDWKD
                     KVEFTGMPSLYLQIDRSIVNSQMQSLLYAIVFVFLLVTLILRSLRLGLHALIPIIATL
                     LVLFGFMGLIGLPLDIATVLVGSVSIGIGVDYAIHMITHYNHEKKHGRDSKRALGHAI
                     RISGRAIVINVLSVSLGFLVLTFSNLVPLQHFGLLVGVTMI"
BASE COUNT         1840 a         1667 c         1699 g         1691 t
ORIGIN      
        1 gtaaggatta aagccaccaa ggctgaaccg gccgggatca ggctgaacaa gtatatcgcc
       61 aatgccggta tctgttccag gcgcgaggcg gatgacctga tcagggcagg cgttataaaa
      121 gtaaacggag aagtagtcac cacattgggg gccaaagtga tgcccggcga caaagtgcag
      181 tatgccgacc agacgctgtc gaatgaaaga aagcagtacc tgttgctgaa caaacagaag
      241 ggctatgtga ccacgacgaa agacccgcac gcaaagaaca cggtcatgga gctgattgcc
      301 ggggcctgca aagagcgcat ctaccctgtc ggccggctcg accgcaacac gaccggcctg
      361 ttgctgttca ccaacgacgg cgaactggcg aaaaagctga tgcacccggc ctcacggatc
      421 ctcaagatgt accatgtggt gctggaccag ccactatcga agcaggacct gatgaaggtg
      481 gccggtggca tcgagatcga cggggaccgg atacaggtcg acgggatcga atatgtggaa
      541 gacgccaaag accggcgtga agtcggcatg gagatccatt cggggcaaaa ccggatcgtc
      601 cggcggatct tcgaatcgct gggttataag gtgacgaagc tcgaccgggt gatgtacgca
      661 gggctcacca agaaaaacct tccccgcggc aaatggaggt tcctctcgga acaggaggtg
      721 atcaacctga aaaagatgcg ttaaaatctt aaagctatgc gcggagatcc caaattatac
      781 cgaatcctgg aagaactggg cattacgttc gattactatg agcatcccgc ggctccgacc
      841 atcgaagagg cgctgaaaaa ctggaagaat atcgactcga cgcactgcaa gaatatcttt
      901 ttccgcaacc acaaaggaaa ccggcattac ctggtgatca tggagcactc gcggccgctg
      961 gccatccacg atctcgaaaa gaaactgaag cagggaaaac tcacctttgc ttcacccgaa
     1021 cggctgatga aataccttgg cgttacgccg ggttctgttt ccttattcgg tctgataaac
     1081 gatgaggccg atcatgttta tgttttcctt gatgaggttt tgagagaggc gaaaaatctt
     1141 agctttcacc cgaacgacaa caccgccagc ctggtgatca ccggcgaagg attccacaaa
     1201 ttcctggagt ggtccgggaa tgggtttgag tttcttgcat tagcatgaga tggcatctca
     1261 aaaccatctc tatctactcc taactaacag tataacagtg cactatgcaa tagttctgcc
     1321 gtaaatgaga tgccatctca tttcgtcaaa tcagtccttt ccttttcagc atcggcgcaa
     1381 catccggctc tttgccacgg aactgcttgt acagcttcat cgggtcgtcg gtgccgccgc
     1441 gggaaaggat gttatcgcgg aacgctttcg ctgtcgccgg atcgaagatg cccgtctctt
     1501 tgaatgcctg gaacgcgtcg gcatccaaaa cttcggccca caggtagctg taatagccgg
     1561 aagaataacc gccggcaaag atgtgggcga aatagggcac acggtagcgg acgatgatct
     1621 cgggcatcat gccgatcttc gacatggaag cgctctcaaa gccaagggca tccataagag
     1681 aggaatcttt cagggtatgg atatccatat cgagataaca ggcggccata tattccactg
     1741 ttgcaaagcc ctggttaaag tgaccgctgg cggtgatctt gtcgatcaac tcctggggga
     1801 taacttcgcc ggtttggtaa tgacgggcgt agagcttcat cacctccgga tcgccggccc
     1861 agttctccat gatctgtgag gggagctcaa cgaaatcgcg tggcgttgaa gtgcccgaaa
     1921 gcttgcggta cctgcattcc gaaagcaggc catgcagcgc gtgcccgaac tcatggaaaa
     1981 gggtttccac ctcttcaaat gtcagcagcg acggaacatc gccggcaggc ttggtaaaat
     2041 tgcaaacagt ggttatgacc ggggagacat tagaatcgcc tgccatgtac tgatcgcggt
     2101 agttgttcat ccatgcgcct ccttccttgc tggcgcgcgg gaagaagtcc atataaagga
     2161 taccgatatg tgacccgtcg gtgtctttga cttcaaagac ctttacttca ggatgatact
     2221 tctggatgtc gtttctttcg gtaaatgtga tgccgaaaag gcgtgtggcc acgtcgaaca
     2281 tgccaagcct gacattttca agcgaaaaat aaggcttcag cgcttcttca tccaggtcat
     2341 acttctgttt gcgcagtttt tcggcataat accaccaatc ccaggcctcg agtttgaaat
     2401 tcccgccttc ggcgtcgatc atcgcctgca gttcggccgc ttccttttta gctacaggga
     2461 gggcagccga ccatacttta tccaggaaat cgtaaacggc agccggattt ttggccatat
     2521 tctcttccag gatataatcg gcatgggtgg catagcccag caggttagcc tttttcaccc
     2581 ggagtgaagc cattttggct gctatttcct tattatcgta ctgattattg ttgtttcccc
     2641 ggttggcata agctttcagc actttttccc gcaattcccg tttagcggaa aactgcagga
     2701 aagggatacg cgaaggggaa tgcagggtaa acagccattt tccgtcgaga ctgtcttctc
     2761 tggcggcaga ggcggcggca tcccttacaa atgcaggaag gccctccaga tcggattcat
     2821 tatcgatcac cagccggaag gcattggttt cggccagcag gttatcgcca aactgcagcg
     2881 acagcatgga aagctctttg ttgatctctc tcagggtcgc ctgcttgtcg tcaggcaggt
     2941 tggaaccacc gcgggcaaac tgtttgtagg tcttttcgag cagcatcgcc tgttcggtat
     3001 ccaggttcag gttatctctc tgttcataaa ccgctttgat ccttttaaac aacccggcat
     3061 tcaacaggat attatcgtaa tgcccggtca ggacgggaga tatttctttg gcaatcgact
     3121 gcagttcatc attggtatta gccgatgtaa ggttatagaa aaccccgctg acgccggcca
     3181 gcaggtcgcc gctgcggtcg agcgctacga tcgtattctc aaagtcgggt gcagccggat
     3241 tggccacaat agcggcaatt tcttcatcat gaacacggat accttcatgg aaagccggca
     3301 ggaagtgttc attcttgatc cggtcgaatg ccgcagtttc gaaaggggtg ccataagggg
     3361 caaagaaggg gttttctgct gttttcatct ttttttcacc tgtggtgcat gatgtaatga
     3421 tccccatcaa caacagggat ataccaaata atgttctcat ttcaacctgg tttttaattt
     3481 acccggcgaa attaggaaga aaacagaagt ataaagcctt attccggttt atttttgatg
     3541 atcttcatgc tggcacgcat catcatgggt gtgacaatga cagtggcaaa actggcgggt
     3601 tcctaaaatg atgaatactc cgaataagag gaagctgttg gcatagaaga agtaattgta
     3661 ataatacttc acacccagga actcggttcc ggtcaaaaaa ccaataacgc ctgccagcat
     3721 catcaatccg ccgaaaatac ctgacaggta acccaggtag ctgataacat cataaagttt
     3781 cataaatatc tgttttggtg aaaatcaaag ttaaatattt tattcacccg gttgttttga
     3841 tattgatttt tttatatttt taaaccttta aacctatata gtcaaatagt ttaaaaatga
     3901 ctaaagacca gaccagggac aagattctgg cagtagctgc caaaatgttc ggaaaatacg
     3961 gattccagaa aacgactgtc gacgaaatag cccgaacggc tcataaagca aaaggatcag
     4021 tttactacta ttacaagagc aaggaagatc tgttcctggc cgttgtcatg caggaaatcc
     4081 agtttcttaa aacaggcctg acccgggtga tagtcgacag ccaggatgcg acaggaatga
     4141 tatgcaatta cctgatgaac agaatgatgc ttatgaagga tgcggtgaac taccacgaat
     4201 cgctgaaagc cgattttgtg gccgatttca gcttcctgaa cgattgccgc gaagatttca
     4261 cccggtttga gatcgacctg atgaaagcca tcattgacag gggaatcagg gagaacaggt
     4321 tccagatcaa ggattcatat gccacagcgc aggtcatcat cctggccatg aaagccatcg
     4381 aaatcccgtt ctaccatcat cataaaatct ccgaatacga gcaaaccata gtcgaactgg
     4441 ttgatattct ggtcagagga ctggaaaaac cctgatattt atattccttt aacaattttt
     4501 aatgaaattc aggattttta tgcttatatt tatactatat ttgactataa aacgtttttg
     4561 gtcatataga ttaattttct tcgccgggaa aaatcatgat gaaatttgaa attggaaact
     4621 cgtaaagtca atgttcatta taataagctt gataattaaa agcaacagat ctattttttt
     4681 tattcataat ctgactataa aacgatatta gtcatttagt atattaatga agacaattgc
     4741 atatagtatt atccggtaca gaaaggccat cataatcctg actctcatca tcacaggtat
     4801 aatggcttac tttttccgat acctcaaagt ggatcccgat gttttcaact acctccccga
     4861 tgatgaccct aaagcaatgc ttttcaggga gataggaaac aaatacgggg gtaattacac
     4921 cgggatcatc ggcctggaga ccaaaaacat cttcaatacg gagacccttg aaaacatccg
     4981 taggataacc gattcggtga agatcatccc gggagtggcg acggttacca gcctcaccaa
     5041 catcatcgat atcaaaggga gcgactgggg gattgagatc ggcaacctgg tcgacaaata
     5101 cgacattccc gatacgcaag aagagctggc agccctgaga acctacactt tatcccagga
     5161 gatgtaccgg ggggccattg tttcggagga tgccaccgtg acagccgtca tggtgaaaat
     5221 tgccgaagga gtggataaaa ttaaagtcgc atcggctatc cgggagaagg tcgaatcgtt
     5281 aaacctttcc gaaaaagtct atttcggcgg tatgcctttt gcgtttaaaa gcctggcgga
     5341 tatcatcatg ggcgatatag ttatcctggc gccaatcacc gcactggtca tcatcctggt
     5401 cctgcttcta tcattccgct catggcgcgg cgtagtcatg ccactggtta cggtcgtcat
     5461 cagcgtcata tggaccattg gcctgatggg cctgttccgt gttgaaatct ccattatctc
     5521 agatgttacc ccggtgattc tgctggccgt tggcagcgct tataccatcc atgtcatcaa
     5581 ccggatacgg caggaaaagg cagagaatcc cgaacggcct atgcaggagg cgctggctta
     5641 catcattata cccgttttcc tggccgccgc caccaccatg gccgggttta tctccttcat
     5701 tttcggctct tatctgacga tgatcagtac tttcggtatt tttaccgcac tgggtgtttt
     5761 ctttgccctg atactttcgc tgactttcgc ccctgccctg ctgtacgctt tcccggaaaa
     5821 aaaacctgct ttaccaggaa aaacaagcga ccggtcggac ctgatcggaa tgatcctggc
     5881 taaaatccag cgacgggtca ttcgacatcc gaaaagggtg atcctgacct gggggatcat
     5941 cctggccggc gccattgccg gcatctttat gatagagcga aatgtgaaca tcctcgatta
     6001 tttccggaaa tcggacccca cccatcaatc cgaaatcctg ttcaagaaca aattcggcgg
     6061 gagcatgcca gtatacatca cggttaaagg cgacgtacag gacccggaaa tgctccggat
     6121 catggccgat atcggggatt accttaaaaa tgacggatat gtgtcgcacg cccagtcggt
     6181 ggctgacctg atcgaagaga tgaacgaagt gatgggagaa ggaaagatga tccccgatga
     6241 aaaagccaag gtagaacagc tctggttcct gctggaagga caggatatca tggaacagct
     6301 ggtgacttat gaaaaagacg aagggcttgt caacactacc accggcaaag gcgacctcgg
     6361 cgaaatgacc ggcctcactg aaaacctgca agcttatatt gaacgccatc cggattggaa
     6421 agacaaggta gaattcacag ggatgccatc gctttacctt cagattgacc ggagcattgt
     6481 caatagccag atgcaatcat tactatatgc catcgtattt gtatttctcc ttgtaacact
     6541 gatcctgaga tcattacggc taggattaca tgccctgatc cccattatcg ccactttgct
     6601 ggtcctgttc ggattcatgg gcctgatcgg gctgccactc gacatcgcca cggtcctcgt
     6661 tggcagtgtt tccatcggga tcggggtaga ttacgccatc cacatgatca cccattacaa
     6721 tcatgaaaag aaacacggga gggattcaaa aagagcgctc ggacatgcca tccgcatcag
     6781 cggaagggcc attgtcatta acgttctttc ggtttcgctg gggttcctgg tcctgacttt
     6841 ctcaaacctg gtcccattgc agcacttcgg cctgctggta ggggtaacga tgatcat
//
LOCUS       DAOQXS010000082         6887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11404_length_6887_cov_4.202137, whole genome shotgun sequence.
ACCESSION   DAOQXS010000082 DAOQXS010000000
VERSION     DAOQXS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11404_length_6887_cov_4.202137"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PKM34_03570"
     CDS             complement(<1..1475)
                     /locus_tag="PKM34_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNQ82696.1"
                     /translation="MENDNTRIYQQTQPIQQQEEGFDYKALFFKLYRHWYFFILTIVV
                     ALIIAFLFNKYTKPVYEVSTTVIIKSDRGNKIDPQQLIGLGFRNDLQNIENEIGVLTS
                     YSLTERTIRNLDFFVAYFEEENFISKELYGNSPYEVVFDTSHLQPVNVEFKVTILSSS
                     KYTIQAEYENVKLYSYSQFKESDQEYPFFKINDTLYFGEQLENEACSFHIVPNPRVKE
                     SNLAGKNLAFVFKDLKSLVGRYQGLNIEPINKDASILKISLRGFNEAKAVAFLNMLTR
                     EYLAQGLEKKNLIASNTIRFIDEQLMDISDSLNYAEMELQDYRSQNIVMDIDFQSQQA
                     VDYMKTLETQKADLSIKNMYYKSLKKYVETNKDVQQGLIVPSAMGIEDPLTTSLIQEL
                     STLFNERAEALLTSTEKNPTIIAYDLRIENTKNALLENITNIVKNSDLTLKDINSRIN
                     ALTDQMTNIPKTQRELLGITRKFTLSDNIYNFLQQRRAEAQ"
     gene            complement(1484..2269)
                     /locus_tag="PKM34_03575"
     CDS             complement(1484..2269)
                     /locus_tag="PKM34_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNQ82697.1"
                     /translation="MNWSTFLTGKAVFLPLIVLLALASCVPMKRVRYLQDEHQKTEAK
                     SDFKKPEPNEYRIHMGDNLYIKINSLDEKSNYFDYTGGINNYYSESAIYLNSYTVNDS
                     GYIEFPLIGKVMVDNLTIEEIQKVLQIEVNEYVKNTVVIVKLANFRISLFGEFNNPGK
                     YVIYQDKINIFEALAMAGDMTGFAKKNTILLVRQTEKGLKKHRLNLNDNSILESEFYY
                     LMPNDMIYAEPVKGKPFAFTEFPYAIIFSVITTSLLLYEVITK"
     gene            complement(2357..3532)
                     /locus_tag="PKM34_03580"
     CDS             complement(2357..3532)
                     /locus_tag="PKM34_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNQ82698.1"
                     /translation="MDLSIYFEPVQLKGYHFSEKTRRKLMGDVVKAYIEPNDFPSLDH
                     VDLAFIGVGEERNCFFNNGCGLGPDAIRKEFYKLYQGNFKLRMVDLGNVRQGHSIEDT
                     YFAVTAIMAELLEHNIVPVILGGSQDITYAQYKAYEKIGRIINIASVDSQFDIGTGQE
                     KLDSRSYMSHIILHQPNYLFNYTNIGFQTYFVDQQAITLMKNLYFDTYRLGIVRNNLE
                     EVEPMVRNADMISVDMGAVRASDAPGNANATPNGFYGEEICQVMRYAGLSDKLTSIGI
                     YELNPRLDRQSLTAQLAGQMIWYFIDGFYSRKGDFPPSKKKELVKFTVNMKDFKDEVV
                     FYRSKKSERWWMEVPIRSKHRERYERHHLVPCSYQDYETACQNALPDRWWQTYQKLM"
     gene            complement(3588..5921)
                     /gene="topA"
                     /locus_tag="PKM34_03585"
     CDS             complement(3588..5921)
                     /gene="topA"
                     /locus_tag="PKM34_03585"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNQ82699.1"
                     /translation="MIKNLVIVESPAKAKTIERFLGKDFTVRSSFGHVMDLSKKGLGV
                     DIEKDFIPIYEISPEKKKVVGELKKAAKEAEVVWLATDEDREGEAISWHLVSALGLDE
                     KATRRIVFHEITKSAILDAIDNPRQIDRDLVNAQQARRVLDRLVGFELSPLLWKKVKP
                     ALSAGRVQSVAVRLIVEREDEIKAFKETSSFKVTAKFTVTIKGAEYKFEAEFPTRFKT
                     REEARKFLEKCIGASYKVTGIETRPSKKSPAPPFTTSTLQQEASRKLGFSVANTMRVA
                     QQLYEEGLITYMRTDSVTLSKMALAMAKEEIIKLYGPEYSKTRQFATKTKGAQEAHEA
                     IRPTYIANAAISGSATQKKLYELIWKRTIASQMSDALTEKTIVNISASTLGGEEFIAQ
                     GEVIKFDGFLKVYRESFDDDSDEEGRSILPPLSVGDQPGMLEMNAEQRFTQPQRRFTE
                     ASLVKRLEELGIGRPSTYAPTISTIQKREYVEKGDVEGFIRTYDLLTLKNNEIKLHEK
                     KDKAGNEKGKLLPTDLGTLVNRFLMQYFQDIIDYQFTATVEKDFDEIAEGKKEWNEMI
                     RKFYKPFHSQIRDTVESTGKFSGERLLGIDPGSGKNVYVKLGRFGPVVQIGETESDEK
                     PRFAGLGKGQSLDTLTLEQALELFKFPRNLGLYENAEMIVAVGRFGPYIKHNNLYYSL
                     TKQDDPALIDQERAISLIELKRKQDREKIIKSFPENENVVVMNGRFGPYIAISKVNYK
                     IPKGTDPASLSLDECLQIAEKQEKSGKKKTIPTRKKS"
     gene            complement(6123..6794)
                     /locus_tag="PKM34_03590"
     CDS             complement(6123..6794)
                     /locus_tag="PKM34_03590"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNQ82700.1"
                     /translation="MSNILMDPIGRLMGLRYKSHPWHGVDIGREAPVIVTAFIEMVPT
                     DTVKYEIDKESGYLRIDRPQKYSNVVPALYGFVPQTFSSTAVAEFCMQKSGRTGIKGD
                     SDPIDICVLTEKEISHGNILVKARPVGGFRMIDRDESDDKIIAVLNNDTVYGHINDIA
                     ELPVLVVDRLKHYFLTYKDLPGKESNVVITHVFGIDEAHEIIRRSMEDYHHKFDTLER
                     ALSNV"
BASE COUNT         1562 a         1669 c         1627 g         2029 t
ORIGIN      
        1 atctgcgcct cggcacggcg ttgctgcaaa aagttgtaaa tattgtcgct cagtgtgaat
       61 tttctcgtaa ttcccagcaa ttccctttgt gttttcggaa tattggtcat ttggtctgtc
      121 agggcgttga tccggctgtt gatatctttc agggtcagat cggaattttt gacaatgttg
      181 gtgatattct ccagcagggc atttttcgta ttttcaatcc tcagatcgta tgcaatgatg
      241 gtcggatttt tttctgtcga tgtcaaaagg gcttctgcac gctcattgaa caaggtggac
      301 agttcctgga tgaggctggt agtgagagga tcttcgatac ccattgccga aggcacaata
      361 aggccctgct gtacgtcctt attggtttcc acgtatttct tcagcgattt gtagtacata
      421 ttcttgatgc tcagatcggc tttctgggtt tccagcgttt tcatataatc aaccgcctgc
      481 tgcgactgga aatcgatatc catgacgata ttctggctcc ggtagtcctg caattccatt
      541 tcggcataat tcagcgaatc gctgatgtcc ataagctgtt cgtcgataaa acggatcgtg
      601 ttgctggcga taaggttttt cttttcgagg ccctgggcaa gatattcccg ggttaacatg
      661 ttaaggaaag ccacggcctt tgcttcattg aatcccctga gtgatatttt gagaatggaa
      721 gcgtctttgt tgatcggctc gatattcagc ccctggtacc gtccaaccag ggatttcaga
      781 tctttaaaga caaatgccag gttttttccc gccaggttgg attctttaac acggggattg
      841 ggtacaatat gaaaactgca tgcttcattc tccagctgtt cgccgaaata gagtgtatca
      901 ttgatcttga agaaaggata ttcctgatcc gattctttaa attgcgaata actatagagc
      961 tttacatttt cgtattcggc ctgtatggta tattttgagg aggaaagaat ggtcactttg
     1021 aattccacgt taaccggctg cagatggctg gtatcaaata caacctcgta agggctgttt
     1081 ccgtaaagtt ctttagagat gaaattttct tcttcaaaat aggccacaaa aaaatcgagg
     1141 ttcctgatcg tcctttcggt cagggaatac gatgtgagga cgccgatttc gttttcaata
     1201 ttctggaggt cattgcggaa acccagtccg atcagttgct ggggatcgat tttattcccc
     1261 ctgtcggatt taatgatgac tgttgtactg acctcatata caggtttggt gtatttgttg
     1321 aataagaagg caatgatcag cgccacgaca atggtcagga tgaaaaaata ccagtggcgg
     1381 tataatttga aaaacagggc tttataatca aaaccctctt cctgttgctg gatgggctga
     1441 gtttgttgat aaatacgggt attgtcgttt tccacggtac tgattatttg gtaatgacct
     1501 cgtagagcag gagcgatgtt gtgataacac tgaatatgat ggcataagga aattccgtaa
     1561 aggcgaacgg tttgcctttg accggctcgg catagatcat gtcgtttggc atcaggtaat
     1621 agaactccga ctcaaggata ctgttgtcgt tcaggttaag gcgatgtttt ttcaatccct
     1681 tttcggtttg cctgaccaga aggatggtat tctttttggc aaaccccgtc atatctcccg
     1741 ccatcgccag tgcttcaaat atatttatct tatcctggta aatcacatat ttccccggat
     1801 tattaaattc cccgaaaaga gagattctga aattggccag tttgacaatc accactgtat
     1861 ttttaacata ttcattcact tcgatttgca aaactttctg gatctcttca atggtaaggt
     1921 tatcgaccat tactttgccg ataaggggga attcgatata acccgaatcg ttgacggtat
     1981 agctgtttaa atagattgcg gattccgaat agtaattatt tatcccgcct gtatagtcaa
     2041 aataattact tttttcatcc agcgaattga ttttgatata cagattatcg cccatatgga
     2101 tccggtattc attcggttcg ggtttcttaa agtcgctttt agcttcagtt ttctgatgct
     2161 catcctggag gtacctgact cttttcatgg gaacacagga tgccagggcc agcaggacaa
     2221 tgagtggcag gaacactgct ttcccggtta aaaaagtaga ccaattcata aaagatagtt
     2281 tcacagacct gtacattatg gtgaaggtgc ttttttagca cggattgcaa aagtaatcat
     2341 aattcgttca tgagaactac atcagtttct ggtaagtttg ccaccagcgg tcgggcaatg
     2401 cgttctggca tgccgtttcg taatcctggt aggagcaggg gaccaggtgg tgcctttcgt
     2461 accgctcgcg gtgcttgctg cggattggca cttccatcca ccagcgttca ctctttttgc
     2521 tccggtagaa caccacctca tccttgaaat ccttcatatt tacggtaaac ttcacaagct
     2581 cttttttctt gctggggggg aaatcgcctt tccggctata aaatccgtcg ataaaatacc
     2641 agatcatctg gcctgccagc tgggcggtca gcgattgccg gtcgagccgc ggattcagtt
     2701 catagatgcc gatggaagtc agcttgtcgc tcaggccggc atagcgcatc acctggcaga
     2761 tctcctcgcc ataaaagccg tttggtgtgg catttgcgtt tcccggtgcg tctgatgccc
     2821 gcactgcgcc catatccaca ctgatcatgt cggcatttct taccatcggc tctacctctt
     2881 ccaggttatt acggacaata cccaggcggt aggtgtcgaa ataaagattt ttcatcaggg
     2941 tgatcgcctg ctggtcgaca aaataggttt gaaaaccgat attggtataa ttgaaaagat
     3001 aattgggctg gtgaaggatg atgtggctca tataactgcg ggagtcgagc ttctcctgcc
     3061 ccgtcccgat gtcgaactgt gagtccacgg aagcgatgtt gatgatcctg ccgatctttt
     3121 catacgcttt gtactgggca taggtaatgt cctggctccc gcccaggatg accggaacga
     3181 tgttgtgctc cagcagctcg gccatgatgg ccgttacggc aaaatacgta tcttcaatcg
     3241 aatgaccctg cctgacgttt cccaggtcga ccatccgcag cttgaaattc ccctggtaca
     3301 atttgtagaa ctccttccgg atggcatcgg gacccagtcc gcagccatta ttgaaaaagc
     3361 agttcctctc ttcacctacg ccgataaagg ccaggtccac gtgatcgaga gaggggaaat
     3421 cgttgggttc gatataggcc tttaccacgt cgcccatgag tttgcggcgt gtcttttcac
     3481 tgaaatgata acctttcagt tgaacgggct cgaaataaat agagaggtcc atgtagcgtt
     3541 tttatccgtg gaaagtatac ggatttgaca cggaaatgtt gccggtttta tgattttttc
     3601 cttgtcggga tggttttctt tttcccgctt ttttcctgtt tttcggcaat ctggaggcac
     3661 tcatccaggc tcagcgaggc aggatcagtc cccttgggga ttttataatt aaccttggag
     3721 atcgctatgt atggcccgaa acgaccattc atcaccacca cattctcgtt ttccgggaat
     3781 gatttaatga tcttttccct gtcctgcttc cgtttcagct cgatgagcga tatggccctt
     3841 tcctggtcga tcagcgccgg atcgtcctgc tttgtcagtg aataatacag gttattatgt
     3901 ttgatataag ggccgaatct gccaacggcg acgatcattt ccgcgttttc ataaagcccc
     3961 aggttgcggg ggaatttgaa aagctccaat gcctgttcca gcgtcagcgt gtcgagggat
     4021 tgccccttgc caagacctgc aaaacgcggt ttttcgtcac tttccgtttc accgatctgg
     4081 acgacagggc cgaaccggcc cagtttcacg taaacattct ttcccgaccc gggatcgatg
     4141 cccagcaacc gttcgccgct gaactttccg gtcgattcga ccgtatcgcg gatctggctg
     4201 tggaagggct tgtaaaactt ccggatcatt tcgttccatt cctttttccc ttccgcgatc
     4261 tcgtcgaagt ctttttcaac cgttgccgtg aactggtaat cgatgatatc ctggaaatac
     4321 tgcatgagga accggtttac cagtgtgccg aggtcggtcg gtaacaactt gcctttttca
     4381 ttgccggcct tatctttctt ttcatgcagc tttatctcat tattctttaa ggtaagaagg
     4441 tcgtaggtac gaataaatcc ttccacatcg cctttttcaa cgtactctct cttctggatg
     4501 gtggaaatgg tcggcgcata tgtcgacggg cggccgattc ccagttcttc cagccgtttt
     4561 accaggctgg cctcggtaaa cctccgctgg ggctgcgtaa accgctgttc ggcattcatt
     4621 tccagcattc cgggctgatc gccgacagaa aggggtggca ggatactgcg cccttcttca
     4681 tccgaatcat catcaaacga ctcccggtaa actttcagaa aaccgtcgaa tttgatcact
     4741 tcaccctggg caataaattc ctccccgccc agtgttgaag cactgatgtt aacaatggtc
     4801 ttttcggtca gtgcatcgct catctggctg gcaatggtcc ttttccagat aagttcataa
     4861 agctttttct gtgttgcgct ccctgaaatg gctgcgttgg caatgtatgt gggccggata
     4921 gcctcgtgtg cctcctgtgc ccctttggtt ttcgtggcaa attgccgtgt tttcgaatat
     4981 tcggggccat acagcttgat gatctcctcc ttggccattg ccagcgccat cttcgacagc
     5041 gttaccgaat cggtacgcat ataggtgatc aaaccttctt cgtagagttg ctgggcaact
     5101 cgcatggtgt tggccaccga aaaacccagt ttgcggctgg cttcctgctg taacgtcgat
     5161 gtggtaaatg gcggagcggg cgattttttc gaggggcgtg tttcgatccc ggtgacttta
     5221 taggaggccc cgatgcattt ttccaggaat ttccgtgcct cttcccgtgt cttgaaacgg
     5281 gttgggaatt cggcttcgaa tttgtattcg gcccctttga tcgttacggt aaattttgcc
     5341 gttaccttaa aagaggaggt ctctttgaaa gccttgatct catcttcccg ttccacgatc
     5401 agacggacgg ccaccgattg cacacgccct gccgacagtg ccggcttaac ttttttccaa
     5461 agcaagggcg aaagctcgaa accgaccagc ctgtcgagca cacggcgagc ctgttgcgcg
     5521 ttcaccaggt cgcggtcgat ctgccgcggg ttgtcaatgg catcgagaat ggccgacttg
     5581 gtgatctcgt gaaagacgat ccggcgcgtg gctttttcat ccaaacccag cgccgatacc
     5641 aggtgccagg atatggcttc cccctcgcgg tcttcatcag tggccagcca gaccacttcc
     5701 gcttctttcg ccgctttttt caactcaccc actacctttt tcttctccgg ggagatctcg
     5761 tagatcggga tgaaatcttt ctcaatatcc acacccaggc ccttttttga aaggtccatc
     5821 acatggccga aactcgagcg gacggtaaaa tccttcccga gaaagcgctc gattgtcttt
     5881 gcttttgccg gcgactcaac aataaccagg tttttgatca tgctctctct ttaattatga
     5941 tgcaagctac aagctgcaat ctacgggcgt tttcacgcga ttcctccttc aaaaaatgcc
     6001 agtgcagact ttgcggccgc aaaagtacaa cttttcttat taatcggatt ttcaggtaaa
     6061 atatacccgg tcgaccgaaa ataattttta attattaatt tataaaacag aaaatcagga
     6121 agttagacgt tggataatgc gcgctccaac gtgtcgaatt tgtggtgata gtcttccatc
     6181 gagcggcgga tgatctcgtg cgcctcatca atcccgaata catgcgtgat gacaacattg
     6241 ctctccttgc cgggaagatc cttgtaggtc aggaaatagt gtttgagccg gtcgacgacc
     6301 aggacgggca gttcggcgat atcgttgatg tgtccgtaaa ccgtatcgtt gttgagaacc
     6361 gcgatgatct tgtcgtccga ctcatcccgg tcaatcatcc ggaaccctcc gaccggacgg
     6421 gctttgacga gaatgtttcc atgtgagatc tccttttccg tcaacacgca gatatcgatc
     6481 gggtcgctat cgcctttgat accggtacgt cccgatttct gcatacagaa ctcggccacc
     6541 gcggtggagc tgaatgtctg cggaacaaat ccgtataatg ccggcacaac gttggaatac
     6601 ttctgcggcc ggtcgatgcg gaggtacccc gactccttgt cgatttcgta cttgaccgta
     6661 tcggtcggga ccatttcaat aaaagccgtg acgatgaccg gtgcctcgcg gccaatatcc
     6721 acgccatgcc agggatgtga tttgtatctc agccccatca gccggcctat cggatccatc
     6781 agaatgtttg acatacctat tttttaattt tcaaattttc aaattttcaa atttccacat
     6841 tgttttcatc caactgttat gccttccttc cagggttttc cgccaga
//
LOCUS       DAOQXS010000083         6841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11495_length_6841_cov_3.907014, whole genome shotgun sequence.
ACCESSION   DAOQXS010000083 DAOQXS010000000
VERSION     DAOQXS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11495_length_6841_cov_3.907014"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PKM34_03595"
     CDS             complement(<1..774)
                     /locus_tag="PKM34_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82701.1"
                     /translation="MKNKVHISGCCVIVILALLTLTGHSQDVRINSGAYVKTMGNASI
                     IIKDADLINNGSFTAGEASSLTFKGSATQMVEGSAPCAFNHLYLDNISGLIFGNSETV
                     NGTLTFINGKITLGSNDLIMGPYAAFSGSNGSNYVITNGVGSLRQWVMNNATDVTYPV
                     GLATEFLPLKLQLTVGSTADEIGARVMDGLYTAYDESDVPAGSLIGDHVVSKTWVLDE
                     TTAGGSNATVNLQWNQTGETTGFDRNLSDLATYSGGAWHY"
     gene            complement(801..2600)
                     /locus_tag="PKM34_03600"
     CDS             complement(801..2600)
                     /locus_tag="PKM34_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82702.1"
                     /translation="MKRMLLFSACLFCCIIISNGQNIGFNDNNSDPHASAIVDIYSTS
                     KGMLIPRVSLDSTLLATPVTNPATSLLVYNTAAVHDVIPGYYYWDGAKWNRLETSSDQ
                     EIYFDPVTKSADATLLKTETLVLASGNISLTLPTISESDNGLKIIVKHVGLHTDLIKI
                     KPSAGQKIDANDSIMLTRWNGKAFLAYNGNWFLETKDSRADNLLDVSATGSFTTIEEV
                     VEFLDAHMCRPVVVRLGAGEYDLDETITIDLPFPVTFQGLSFGATIINCPSGDTAFNL
                     KSECYFKMLTMYGGETPGVGIDLSGEDVYYEIKDGYLYGFSKGIVISNAADIWLFEVD
                     FDSCTVAGVEIAAPDTAKVVFRTSECDYYNCDIANNLVSAGEETEISILSSTFYNFTI
                     DQIGINYLPPVDNNNFRFASMIIQNNSFNNIGNFCSGFDFSRTDGRDANVFMENNAGY
                     ESKAPHCKINVANNTTWTTITSANTWYKYVGFSNYNSTTTKWTIGNNRITYQTENIRD
                     AVIFLSGNVKTNSTSARVINIAIVKNGNSTTRYGETTIRIPSSNANQSFQWSTNVYVE
                     NMDKNDYLELWITSASSGDVLTIDDLNWYTSTH"
     gene            complement(3013..4998)
                     /locus_tag="PKM34_03605"
     CDS             complement(3013..4998)
                     /locus_tag="PKM34_03605"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82703.1"
                     /translation="MLTLIFRFRYFILLSFLGGMMAVQGQDAPVTTAATIGGANPGNI
                     SVPVTVTGFTGISAISLSIEYDYAVMHFTGATPNPALPSFPTGEIDLGTGIRRISMGW
                     FGPSTSLADGSAIITLHFTYIGGITGLDWYDNGPSCEYADANYTVLNDIPKENFYIDG
                     HVCGIIGTPGPVSGPDSLCAGQTGIVYSISPVPNATEYTWTVPENVIIISGQNTTSVF
                     VEFLINAASGNIGVSTGNPCGTGPSAVKPVTVNPVPVANAGNDTTINYGTSITLHAAP
                     GGNGSYAYHWAPEELLVDPDVQNPQTVILTETSMFTLHVTNLATLCEESDMLFVTITG
                     GPLSANPTATPPLICRYSSSWLFSNAGGGSGNYNYLWTCVPPCDPPWSSTEANPFVAP
                     DVSQRYHLTVNDGFTTIEDSVFVAVAELPTSTLTGGDTLCGPGTFTTLQVDLTGTPPW
                     FFTYSFGNTTVFVNDVLETPYYITTGDPGDYMISLVEDANCIGITYGTAMVRAFPIPA
                     KPEITVYGTELISSSCCGNQWYKNGVLIPGAGGQTYQVTQNGEYFVIVTLNSCSSEPS
                     DTVDMIVGIPEYLSPKLEIFPNPSKGLITINTGQQVDETAALELYSSSGYLIKKNLLA
                     VTHKGTVTIDISSFPEGLYFIILKTSTRISSGKLIKD"
     gene            complement(5062..>6841)
                     /locus_tag="PKM34_03610"
     CDS             complement(5062..>6841)
                     /locus_tag="PKM34_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82704.1"
                     /translation="LTGYSGDIVYWERSINDGAWDILPGTAGQAQFEDVPTIEGTWSY
                     RVKVEKPGCQPVYSVPGDITVELLAIWTGAVNNSWDDPGNWNIAGVPDENLDGLVPQT
                     ETGMYPVINGYENGKNLLIGPGASLILSKEGALTLSGYLKNDGLFSMESDTGASASLL
                     DNGISGTGVFVSSVIYTPGLHHQISSPVIHSAKNAFGNTDLFEWNEPQNEYIPVPDDD
                     DTLEVLKGYLAINNDQVMNPAVFEGTGLFSGIYSADFTNNGAGNGHAGGFNLAGNPYP
                     SAMNWNDAENVTLSQVDPALYVLNGEAGNFGTFIRGDHASSTLGIDSIIGTHQGFFVH
                     VNHASDSGSLTVNNGARFHNPQPLRAATAAHSDDPVLKLKVSGPEGSFRDEIIVRFHS
                     EASEYFDIQYDAYDLEGFQNAPQVFTRCADTLRLAVNTYPSLDANRSVVMGFQSGEAG
                     EFTLAVQELLNFGTDTLVFVEDLILDTTFRVTESFQYVFEGTPSDPVDRFRLHFIVDP
                     VFEDEGSGLPEISITLAGQIMRVNFGGESQCGYFELFDLTGKRLMSQRVTGPVAMIHF
                     PLESGMYIIRINIQKAVLVKKIIACF"
BASE COUNT         1726 a         1630 c         1738 g         1747 t
ORIGIN      
        1 ataatgccag gcgccaccgg agtaagtggc aagatcggag agattgcggt caaatcccgt
       61 cgtctctccg gtctggttcc attgcagatt aacggtggcg ttgctgccgc cggcagtggt
      121 ttcatcaaga acccatgtct ttgaaacgac atggtcgccg atcagcgaac cggccgggac
      181 atcactttca tcatacgccg tgtagaggcc gtccatcacc cttgcgccga tttcatctgc
      241 cgttgaacct actgtcagct ggagcttcag gggaagaaat tccgttgcca gcccgaccgg
      301 gtaggtcaca tcagtggcgt tgttcatcac ccattggcgc aatgacccta caccattagt
      361 tatcacataa tttgaaccgt tgctgccgga aaatgcagca taaggaccca taatcaggtc
      421 attgctgcca agggtgattt taccgtttat gaaagttagt gtcccgttga ccgtttcact
      481 gttaccaaaa atcaatccgc taatattatc cagataaagg tgattgaatg cacagggggc
      541 agagccctct accatttgag tggctgaacc tttaaaagtg aggctgctgg cttcaccggc
      601 cgtaaagctg ccgttgttga tcaggtccgc atcttttatg ataatgctgg cattgcccat
      661 ggttttcaca taagcgccgg agttgatgcg tacatcctga gaatgtccgg ttaaagtgag
      721 cagggccaga ataacgatta cgcaacatcc tgatatgtgt accttatttt tcatggctaa
      781 tggtttcatg ggtttaataa tcaatgggtg ctggtatacc agttcaggtc gtcgatggtc
      841 aatacatccc cgctgctggc agaggtgatc cagagttcca gatagtcgtt cttgtccatg
      901 ttttcaacgt acacattggt cgaccattga aatgattggt tggcattgct ggaggggata
      961 cggatcgtgg tttcaccata tcgggtagtg gaatttccgt ttttgacaat ggctatgttg
     1021 atcaccctgg ctgaagtact gttggtttta acatttcctg aaagaaaaat gacagcgtcc
     1081 cttatatttt cagtttggta ggtaatccgg ttgttgccga tggtccattt tgtagttgtg
     1141 ctgttataat tgctgaaacc aacgtattta taccaggtat tggctgatgt gattgttgtc
     1201 caggtagtat tattggccac attgattttg cagtgcggcg ctttgctttc atatccggca
     1261 ttgttctcca tgaatacatt ggcatcgcgg ccatcggtac gcgaaaaatc aaatcccgaa
     1321 cagaaattcc cgatattgtt aaaggagtta ttctggatga tcatcgaggc aaaccggaaa
     1381 ttgttgttat cgacgggagg gagataatta atgccgatct gatcaatagt aaaattgtaa
     1441 aatgttgaac tcaggatgga tatctcagtc tcttcacctg ccgacaccag gttgttggcg
     1501 atatcacagt tgtaatagtc gcattcggaa gttctgaata ccactttagc tgtgtcgggt
     1561 gcagcaatct ccacaccggc tacggtgcat gagtcaaagt ccacttcaaa aagccagata
     1621 tccgcggcat ttgatataac tattcctttt gaaaaaccat agagataacc gtccttaatt
     1681 tcatagtaaa catcttcacc gcttaaatca ataccaactc ccggggtttc tccgccatac
     1741 atggtaagca ttttaaaata acactccgat ttcaggttaa aagccgtatc tcccgaagga
     1801 caattaataa tggttgcacc gaacgacagg ccctggaatg tcaccggaaa aggcaggtca
     1861 atagtaatgg tctcatccag gtcatattca ccggcaccca gacgaactac taccggacgg
     1921 cacatatggg catccagaaa ttccactacc tcttcaatcg ttgtgaagct gccggtcgcg
     1981 cttacatcca ggaggttatc cgcacgcgaa tccttggttt ccaggaacca gttgccgtta
     2041 taagcaagga aagctttgcc attccaccgg gtgagcatga tgctgtcgtt ggcatcgatc
     2101 ttttgcccgg cggaaggttt tattttaatc agatccgtat gcagacctac atgttttaca
     2161 atgatcttca gtccgttatc tgattcggat atggtaggca gggtcagaga gatgttccct
     2221 gaagccagga ccagggtttc ggtcttcagc agtgtagcat cagcagattt ggtcaccgga
     2281 tcaaaataga tttcttggtc agaagaagtt tccagccggt tccatttggc gccatcccag
     2341 taataataac cgggaatgac atcatgaaca gcagcggtgt tgtaaaccaa aagactggtc
     2401 gcggggttgg ttaccggggt tgccagcagc gtactgtcga ggctgaccct ggggataagc
     2461 attcctttgc tggtcgagta aatatccact atcgccgaag cgtgcggatc ggagttgttg
     2521 tcgttgaatc caatgttctg tccattgctg atgatgatac agcaaaacaa acatgctgag
     2581 aagagaagca ttcgtttcat ggtgtagttt tttatgttta gttatgtgat aacttctctt
     2641 tacaatgcca gccataaggc cggacgataa tagccggggg gatctatcag gtggtggtta
     2701 aaaaaaatcc ttcaaagcac aatcgtgtcg ttagtgttga aggagtaatg tcagctaaat
     2761 cagtcgaatg tatttctttc aatcgtcagc gcgaaacaca tcattagtcg acgccgcatt
     2821 ggcaaaggga gtgccaaaat gaagagaggg ataggggaag ttttgtattc aacagtatat
     2881 cagtagatta tatgagtgaa gaatgaatcg tgaatagtgg aaaatatttg ggagaattcc
     2941 tgcccccaaa aaaaccgcac acgtgtgcgg aaataatggt ggtccgttaa ttccaagaat
     3001 atgtggttag aatcagtctt ttatcagttt gcctgaactg attcgggtag aggttttcag
     3061 aatgatgaaa tataagcctt ccgggaagct tgaaatatca atggttacag tgcctttgtg
     3121 ggtgacggca aggagattct ttttgataag atatccggag gatgaataca gctccagggc
     3181 tgcggtttca tcaacctgtt gaccggtatt gatagtgata aggccttttg aaggattcgg
     3241 gaagatttca agttttgggc tgaggtattc cggtatcccg acgatcatgt cgactgtgtc
     3301 cgatggttcg gagctgcagc tgttcagcgt cacgatcaca aaatattccc cgttctgggt
     3361 cacctggtag gtctgaccgc cggcaccggg gatgaggacg ccgtttttat accactggtt
     3421 gccgcagcag gaactggaga tcagttctgt tccgtaaacg gttatttccg gcttcgccgg
     3481 gatgggaaat gccctgacca tggccgtccc gtaagttatc ccaatgcagt tggcatcctc
     3541 aaccaatgat atcatatagt cgccgggatc acctgttgtg atgtaatacg gtgtttccaa
     3601 tacatcattc acaaaaacgg tcgtgttccc gaacgaatag gtgaaaaacc agggtggggt
     3661 tcccgtcaga tcaacctgca gggtggtaaa agtcccgggc ccgcataagg tatccccgcc
     3721 ggtgagtgtg cttgtcggaa gctctgctac ggctacgaac accgaatcct caatggttgt
     3781 aaagccgtcg ttcacggtca ggtgatagcg ttgtgataca tcgggcgcca caaacggatt
     3841 ggcttcggtg gagctccagg gcggatcgca cggcgggacg caggtccaca ggtagttgta
     3901 gttgcccgaa ccgccaccgg cattggaaaa aagccacgaa gaagagtaac ggcagatcag
     3961 gggcggcgta gctgtcggat tggcgctcaa cggcccgccg gtgatcgtca caaaaagcat
     4021 gtccgactcc tcgcaaaggg tggcgagatt cgtcacatga agcgtaaaca tactggtttc
     4081 ggtaagaata accgtctgtg ggttttggac atccggatcg accagcagct cttccggcgc
     4141 ccagtggtaa gcataactgc cgttgcctcc cggcgcggca tgcaatgtga tcgaagtgcc
     4201 gtaattgatg gtcgtatcgt tgccggcatt ggcgacaggc actgggttca ccgtgacggg
     4261 ctttacggcc gaagggcctg taccgcaggg attgcctgtg ctgacaccga tattgcccga
     4321 cgcggcattg atcaggaatt cgacgaaaac agaggtcgta ttctgcccgc tgatgatgat
     4381 cacattttca gggacggtcc aggtatattc cgtggcattc ggcacaggcg atatgctgta
     4441 tacaatacct gtctggcccg cgcaaaggga atccggcccc gataccggcc ccggcgttcc
     4501 gatgatcccg catacatgtc catcgatgta gaaattttct ttggggatat cattgagaac
     4561 ggtgtaattg gcatcggcat attcgcagga ggggccgttg tcgtaccagt ccaggccggt
     4621 gatgcccccg atataggtga aatgaagcgt gatgatcgca ctaccgtcgg ccaggctggt
     4681 cgatggaccg aaccacccca tgctgatcct ccggataccc gttcccaggt cgatttcacc
     4741 ggtcgggaat gaaggcaggg ccggattcgg cgtcgctccc gtaaaatgca tcaccgcgta
     4801 gtcgtattct atggagaggg aaattgcgct gatgccggtg aaaccggtca cggtaaccgg
     4861 aacactgata ttcccgggat tcgccccgcc aatcgtggcg gcagtggtga cgggggcgtc
     4921 ctgcccttgc accgccatca tcccgccaag gaaagacagg aggataaaat aacggaacct
     4981 gaatatgagg gtaagcatgt ttttttctgt aaagataaat acattttacc cagaatgaaa
     5041 tcatccgtac cggtcaggat ttcaaaagca agctatgatt tttttgacaa gaacagcttt
     5101 ttgaatatta atgcggatta tatacatgcc actttccagc gggaaatgga tcatagcaac
     5161 cggccctgtt accctctggc tcatcagtcg ttttccggtc aggtcaaaca attcaaaata
     5221 accacactgc gactctccac cgaaattaac ccgcattatc tgccctgcta atgttatgga
     5281 aatctcagga agaccacttc cttcatcttc aaaaacagga tccacaataa agtgaagcct
     5341 gaaccggtcg accgggtcgg acggtgttcc ctcaaaaaca tattggaatg attcggtaac
     5401 ccggaatgtc gtgtcaagga tcagatcttc cacaaatacc agcgtatccg tgccaaaatt
     5461 aagcagttcc tgtaccgcga gggtaaactc tccggcttca ccggattgaa aacccattac
     5521 aacagaccgg ttggcatcca gcgaaggata agtattgaca gccagtctca gggtatcggc
     5581 acaccgggtg aatacctgcg gggcattttg gaacccttcc agatcatagg catcatattg
     5641 aatgtcaaag tattccgatg cttccgagtg aaatcttaca atgatttcat ctctgaatga
     5701 accttcaggc ccggatactt ttaatttcag gacaggatca tcagagtgtg ctgccgttgc
     5761 cgcccgcaaa ggctgagggt tgtgaaatct tgccccattg ttcaccgtca gagatccgga
     5821 atcggaagca tgatttacat gcacaaaaaa accctggtgg gtgccgataa tgctgtcgat
     5881 acccaatgtg gaactggcat ggtcgccgcg aataaacgta ccgaaattgc cggcctcgcc
     5941 attcaaaacg tacagggccg gatcaacctg cgacagagtc acattctccg catcattcca
     6001 gttcatggcc gaaggatacg gatttccggc gaggttaaaa ccgccggcgt gtccgtttcc
     6061 ggcgccgtta ttggtgaaat ctgcagagta aatgccggaa aacaggccgg tcccttcaaa
     6121 aaccgccgga ttcattacct gatcattatt tatggcaagg taccctttta atacttcaag
     6181 tgtatcgtca tcatccggca ccgggatata ttcattctgc ggctcgttcc attcaaacag
     6241 gtccgtattc ccgaatgcat tttttgcaga atggattacc ggggatgaaa tttgatgatg
     6301 taaaccaggg gtataaatta cggaactgac aaacacccct gtaccggaga tgccgttatc
     6361 cagtaatgaa gcgcttgccc cggtgtcgga ctccattgag aacaggccgt catttttaag
     6421 atatcccgac aaggtcaaag ctccttcttt tgagaggatc agggatgcgc cgggaccgat
     6481 cagcaggttt ttgccatttt cataaccatt gatcacagga tacatcccgg tctctgtctg
     6541 cgggaccagg ccatcgaggt tttcatccgg cactcccgcg atgttccagt tccccggatc
     6601 atcccaggag ttgttgacag cgccggtcca aatagcaagc aattccacgg tgatatcgcc
     6661 cggtactgag taaaccggct ggcagcccgg cttctccact ttaacccggt acgaccaggt
     6721 tccttcgatc gtgggtacat cttcgaactg agcctgtcct gcagtgccgg gaaggatatc
     6781 ccaggcgccg tcgttaatac ttctttccca gtagacgata tccccgctgt aaccggtcag
     6841 c
//
LOCUS       DAOQXS010000084         6819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11536_length_6819_cov_3.781195, whole genome shotgun sequence.
ACCESSION   DAOQXS010000084 DAOQXS010000000
VERSION     DAOQXS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11536_length_6819_cov_3.781195"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..674)
                     /locus_tag="PKM34_03615"
     CDS             complement(168..674)
                     /locus_tag="PKM34_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82705.1"
                     /translation="MKKILFITIIIATSIFSAHSQDRPGISIFLHQTGMYYSSDNITS
                     EGLGLGIGAHFLHKSQIAGQVDANILWGNGNAITTRIAAGYEKKGKWAPAIYATCNLL
                     WGQRTEILAENGDRPPLPAWAAGVRVTPLKFRTGRGYVSALEFGYGVGPAKAMSLEFS
                     VLSIGIRF"
     gene            complement(671..1678)
                     /locus_tag="PKM34_03620"
     CDS             complement(671..1678)
                     /locus_tag="PKM34_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNQ82706.1"
                     /translation="MKMNRLLNLTIVTFLALLVACGEKEGDRSGFEEATVVAQQVNGE
                     NLMNSVAELVDIHLNDIPIDNSECPPGDLFPTDHLTRNAAVGYISEEFRKMGYIPDTA
                     VLGEGAYITYNVFAEWPGTSDAEEVILVGSHLDAFYGGADDNTSAVAAMLEAARIARN
                     FSFSHTIRFIAFDLEELGSIGSTRYVEGGLAGDVKTAIVMDLIGYRSFEPGSQKSVMG
                     IRLPDVGDYLMVIGNRDSEEITRQITDMANSQSLSKALGVLAPGDGAYFLSQALMRSD
                     HGLMWYKGIPAVFFTDGANFRNPNYHLASDLPETIDADFLTGNTKVLMAAIALLAEVQ
                     L"
     gene            complement(1696..2238)
                     /locus_tag="PKM34_03625"
     CDS             complement(1696..2238)
                     /locus_tag="PKM34_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82707.1"
                     /translation="MKKIISSLVALLFFLPLFAQEKQDKKSFEVTAGYSMLFYQSAYN
                     LQNASGIEASIGREISDPLRWQAGLRVGLDPLLPEVFGRILLQEEVGSWRPQIGLELG
                     VTGRAKFEDGAFLLRETREAMQTGIDAGYLAIHTAPLSFRISKNWNLSIAEIDFGTHF
                     TRIGRTLRAQVTLISAGMKF"
     gene            complement(2310..2942)
                     /locus_tag="PKM34_03630"
     CDS             complement(2310..2942)
                     /locus_tag="PKM34_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ82708.1"
                     /translation="MDKNVLERKTLFVVLLTAITMIVEIFFGLTTKSMALLADGIHMG
                     SHVLAIGLSWIAYVFIRRSKASGRFKGNPDKVLSLSGYSSGLMLLIFAFFILSEAIKR
                     SVNPLQIDYKEAIIVACIGLIVNIISAFILHHDKSQSDHNIRAAYLHVLADALTSVSA
                     ILGLTAALIWNIPIIDAMAAILSSLVIIRWSVGLILDSGKSLLDIHNQKK"
     gene            complement(3014..4426)
                     /locus_tag="PKM34_03635"
     CDS             complement(3014..4426)
                     /locus_tag="PKM34_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ82709.1"
                     /translation="MKILLIAPCQVDQQRPNSMDIPQLTLSLIAALTPPDHEVEIVEE
                     VYGETVNFDGDYDLIGISIMTQTAIRGYQVANEFRKRGKTVVFGGIHATSMPDEAIRY
                     GNAVVIGEAEGLWDIVLEDAKHNRLKSFYKLDKLPDLQKHIRPRRDLIKCSSGRFAIA
                     PIETTRGCPYNCDFCSVSRFFGTRQRHKPIPDIIADIESCREKNLFFLDDNITGDKKY
                     ARELFREMIPLNKIWVGQASVQVANDPELMNLAYRSGCRALLIGFESMSDSGISQYRK
                     TLKTIDENVSAVKKLQDNGIMTMASLIFGLDTDTPDVFDVAHDFLTRSKAAFFQACVM
                     TPYPGTPVFNKLRNEGRILTDDWTRFDASKVIIRPENLSPEELLEGYNKMKYHFYSNK
                     SILQRSYPNIKIGFYEALLYFTLNKGYQKKNNPSVFSQVYENPSGHPVDFNVAKYVRP
                     LINGHAPVFSEKLSEVNRIR"
     gene            4751..6610
                     /locus_tag="PKM34_03640"
     CDS             4751..6610
                     /locus_tag="PKM34_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNQ82710.1"
                     /translation="MKKKIKFCLVYRDMWQSSGKYQPHVGQLKAVAPLIIEMECFSRV
                     ETNGGAFEQVQLMYGENPNVAVREWTKPFNEAGIQTQMLERALNGIRMYPVPKDVRRL
                     MFTVKKAQGVDIARSFCGLNDHRNLELSIKYAKEAGMISQAALSITFSPVHTVNYYMD
                     VVKKMVKYGAEEICLKDMAGIGRPVMLGELVKKIKKKHSHIKVQYHGHSGPGLSMASI
                     LEVARAGADYIDVAMEPLSWGMVHPDILSVHALLRDAGFKVPDINMNAYMKVRSMTQE
                     FIDDSLGLFIDPRNKQLSSLLIGSGLPGGMMGSLMADLKGAQRSINTSLKGRGIPELS
                     EDELMVRMFNEVEYIWPKLGYPPLVTPFSQYVKNMAIMNILQMAGGKERYTMIDNNTW
                     DMILGKAGKLPGPLAPEIVTLAGEQGKEFYQGVPQDAFPDELEKYRKEMTANNWDTGP
                     DEEELFEFAMHDKQYRDFKSGIAKTRFEAELAKAKELAEGPKNMPAAPEPAPAPAPVK
                     EKPVEEKPKEVDPDIREITAPAKGQIIYNLFGEDTEFSTIGEHISPGDRLCYIQTSAY
                     IGEITCPYNGIISEIVVQQGVQVKKGEVIFRIKEVVGEIKPKTPKKKSQTAGK"
     gene            complement(6639..>6819)
                     /locus_tag="PKM34_03645"
     CDS             complement(6639..>6819)
                     /locus_tag="PKM34_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842569.1"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNQ82711.1"
                     /translation="QRLIDYKSQGKEFEMAIPTPEHYLPLLYVLALKEGNEEVGLFND
                     KAIAGSLTMTSAIVR"
BASE COUNT         1825 a         1612 c         1672 g         1710 t
ORIGIN      
        1 acggcgggca gtacgcattc atcataccgg agaaaaaatt agtagtcgtc ataacgtctc
       61 tttcccaggt tgacgatgac gtcaatatcc cgattgagcg gatcgttgat attgtcgaca
      121 ggatagtggc agcatcgcat tgaaaaaggg ttgctgcccc ggtaagatca aaatcttatc
      181 ccgatggaaa gcacagagaa ttccaggctc atcgcctttg caggtccaac gccataacca
      241 aattccaaag ctgagacata tcctcttccg gttctgaatt ttaacggtgt taccctgacg
      301 cctgccgccc aggctggtaa aggaggtctg tctccatttt ctgcaagtat ctctgttcgc
      361 tgaccccata acaggttgca ggtcgcatag atcgccggtg cccacttccc ctttttctca
      421 taacctgcag caattctggt tgttatcgca ttcccgttgc cccacagaat attggcgtca
      481 acctggccgg caatctggga tttatggaga aaatgggccc cgattcccag cccgagacct
      541 tcgctggtga tattatctga agagtaatac atcccggtct ggtgaaggaa aatgctgatc
      601 ccgggtctgt cctgagaatg ggcagaaaat atgcttgttg cgataataat ggtaatgaat
      661 aaaatctttt tcatagctgt acctccgcta gtaaggcaat ggctgccatc agaacttttg
      721 tgttaccggt cagaaaatct gcatcaatgg tttcaggaag gtcggaagcc agatggtagt
      781 taggattcct gaaattggct ccatctgtaa agaagacggc agggattcct ttgtaccaca
      841 tcaatccgtg gtcactccgc atcagggcct gcgacaggaa ataagctcca tcgcccgggg
      901 ccaggactcc caatgccttt gacaaacttt ggctattcgc catatccgtg atttgcctgg
      961 taatttcttc agaatccctg ttgccgataa ccatcagata atcacccaca tcaggcagac
     1021 ggattcccat aacactcttc tggcttcccg gttcaaaaga acgataaccg atcaaatcca
     1081 tgacaatggc tgttttcaca tcgccggcca gtccaccttc aacataccga gtactgccaa
     1141 tcgaacccag ttcttcgagg tcaaatgcta tgaaccggat cgtgtggctg aaactgaaat
     1201 tccgggcaat tctcgcagct tcgagcatcg cagcgaccgc tgaagtgttg tcatcagccc
     1261 ctccgtagaa ggcatcaaga tggctcccca ccaggataac ttcctccgca tctgatgtcc
     1321 ccggccattc ggcaaaaaca ttgtaggtga tgtatgcacc ttcgcccagc acggcggtat
     1381 cgggaatata acccatcttc cggaattctt cgctgatata acccacagcg gcattccgcg
     1441 tcaggtgatc ggtcgggaac aagtcgccgg gcgggcattc gctgttgtcg atcgggatgt
     1501 cgttcagatg tatatcgacg agttctgcaa cgctgttcat caggttttct ccgttcacct
     1561 gttgtgccac aaccgtagcc tcttcaaatc ctgatctgtc accctctttt tcaccgcagg
     1621 ccactaacag tgccaggaaa gtgacgatgg ttaagttcaa taatctattc attttcatca
     1681 caataaaatt aatccctaga atttcattcc ggctgagatt aaggtcacct gagcgcgcag
     1741 ggttcggcca atccgtgtaa aatgggtgcc gaaatcaatt tccgcaatgc tgagattcca
     1801 gtttttactg atcctgaacg acaggggagc ggtgtggatg gccaggtagc ctgcatcgat
     1861 accggtctgc atagcttccc gggtttcccg aagaagaaag gccccatctt caaactttgc
     1921 cctgcctgta actcccagtt ccaaaccgat ttgcggacgc caggaaccga cctcctcctg
     1981 aagcaagatc cggccaaaaa cttcgggcag taaaggatct aatccaactc tgagcccggc
     2041 ctgccatctt aaaggatcgc tgatttctct cccgatggaa gcttcgatac cggaagcatt
     2101 ctgcagattg taagccgact ggtagaagag catgctatag ccggccgtaa cctcaaagct
     2161 ttttttatcc tgtttttcct gtgcgaacaa aggcaggaaa aaaagcaatg ctacgagtga
     2221 agaaattatc tttttcatat taacttttaa tgatccggga atgataacca atgccaatga
     2281 aattctcaac gggtgagggc attatgttgt tatttttttt ggttatggat atcgagtaaa
     2341 gattttcccg aatcaaggat cagccctacc gaccagcgga tgatcacaag tgagctgaga
     2401 atggccgcca tcgcatctat aatcgggatg ttccagataa gcgccgctgt cagacccagt
     2461 atagctgaaa cactcgtaag ggcatcagcc agcacatgca ggtaagcagc ccggatgttg
     2521 tgatccgact ggcttttgtc gtgatgcagg atgaatgcgc ttataatatt gacgatcagc
     2581 ccgatacatg ctacaatgat agcttccttg taatctatct gaaggggatt gacgcttctt
     2641 tttatggctt ctgaaaggat aaaaaaggcg aagatgagca acatcaggcc actgctataa
     2701 cctgagagcg acaggacctt atccggattg cctttgaatc tgccgctggc cttcgatcgc
     2761 ctgataaaaa cataggctat ccagctcagt ccgatggcca gcacatgcga tcccatgtgg
     2821 atgccgtcgg ccagtaaggc catcgatttt gtcgttaatc cgaaaaaaat ctcgacaatc
     2881 atcgtgatgg ccgttaaaag cacgacaaac agggttttcc tctccagtac attcttgtcc
     2941 atggcaccag caaatcagga ttgcgacgga aattagtgcc ggtaaaatta caaaaggatt
     3001 cgggatatta tccttaacgg attctattaa cctccgacaa tttttcactg aaaaccggag
     3061 catgcccgtt tatcaaaggc ctgacatact tcgccacatt gaaatccacc ggatgaccgg
     3121 atgggttttc atatacctgt gaaaacaccg acggattgtt ttttttctgg tatcctttat
     3181 ttaaagtgaa atacaggaga gcttcataga agccgatctt aatgttggga taagaccgtt
     3241 gaaggatgga tttgttcgaa tagaaatgat atttcatttt gttgtagccc tccaacaatt
     3301 cttccggtga aaggttttcc ggacggatga tgactttcga agcatcgaac cgggtccagt
     3361 cgtcggtgag aatccgtcct tcgtttcgta atttgttgaa taccggtgtc ccgggataag
     3421 gggtcattac gcaggcctgg aaaaatgcgg cttttgagcg ggtgaggaaa tcatgggcaa
     3481 catcaaacac atccggtgtg tcagtatcga ggccgaagat cagcgatgcc atggtcatga
     3541 tcccgttgtc ctgaagcttt ttcaccgcgc tgacgttttc atcaatagtt ttcagcgttt
     3601 tccggtactg gctgatcccc gaatccgaca tcgattcgaa cccgatgagc aaggccctgc
     3661 atcccgatct gtaagcgaga ttcataagct ccgggtcatt ggcgacctgt accgatgcct
     3721 ggccaaccca gatcttattc aacgggatca tctccctgaa cagttcccgg gcatattttt
     3781 tatccccggt aatgttgtcg tcgaggaaga acaggttctt ctcacggcat gattcaatgt
     3841 cagcgataat atcgggtatg ggtttatgcc gctggcgggt gccgaaaaac cgcgaaaccg
     3901 aacaaaagtc gcagttataa gggcaaccgc gggtggtctc aataggggcg atggcgaatc
     3961 ttcccgatga acatttgata agatccctgc ggggccttat atgtttttgt agatcaggaa
     4021 gtttgtcgag tttgtagaac gatttcagcc ggttgtgttt agcatcttcc aggacgatat
     4081 cccacagccc ttctgcctcg ccgataacga ctgcgttacc atacctaata gcctcatcag
     4141 gcatcgatgt agcatggatc cccccgaaaa ccactgtttt acctcgtttc cggaattcgt
     4201 tggccacctg gtacccgcgg atggccgtct gcgtcatgat cgaaatcccg ataaggtcgt
     4261 aatccccgtc gaaattgacc gtttctccat acacttcttc cacaatttcc acttcatggt
     4321 cagggggtgt cagggctgct atcaaagaaa gtgtcaactg gggaatgtcc atcgaattcg
     4381 gacgttgctg atcaacctga cagggtgcaa ttagtaatat tttcatcgtg ataattatgc
     4441 tttgcttaaa agctgtcgcc tcatttatcg cagagcatag gaaatattac cggatcaaat
     4501 gcagtatgac tgtaatgtcg atatcctctg taactttaag gtaatgtaac gaatataata
     4561 acgggctaaa agtaaataaa ttataaaagc ggtgatttag aataatggat atacgaattt
     4621 ttttaaaaga cgaaaataaa tacgcctgcc ggcgttgata tgatttaatc ctgataattt
     4681 tgcggaacct gtgaaaatgc gattttccgc aaatgactat taaattacgg accaatagat
     4741 ttataaccga atgaagaaaa agataaagtt ttgcctggtt taccgcgaca tgtggcaaag
     4801 ctcgggaaag taccagcccc atgtcgggca gcttaaggcg gtagcccctt taatcattga
     4861 aatggaatgt ttctcgcggg tggaaaccaa cggaggcgcc ttcgagcagg tacagttgat
     4921 gtatggagaa aatccaaacg tcgccgtcag ggaatggacc aaacctttca atgaagcggg
     4981 tattcagacg cagatgctgg aaagggcttt gaatggtatc aggatgtatc ccgttccgaa
     5041 ggatgtaagg cgattgatgt tcacagtgaa gaaagcacag ggagtcgata ttgcccggtc
     5101 gttttgcgga ttgaacgatc accggaacct tgaactatcc atcaaatacg cgaaggaggc
     5161 ggggatgatc tcgcaggccg ccctctccat cactttttcc ccggttcata cggttaatta
     5221 ctacatggat gtggtgaaaa agatggttaa atacggcgct gaagagatct gcctgaagga
     5281 tatggccggg atcggacggc cagtgatgct gggcgagctg gtcaaaaaga tcaaaaagaa
     5341 gcactcccat atcaaggttc aataccacgg acattcaggc ccggggcttt cgatggcttc
     5401 gatcctggag gtggccaggg ccggtgccga ttatatcgat gtggcgatgg aaccgctgag
     5461 ctgggggatg gtccatcccg atatcctctc cgttcatgcc ctcttgcgcg acgcaggctt
     5521 caaagtgcct gatatcaaca tgaatgctta tatgaaagtc aggtcgatga cgcaggaatt
     5581 tatcgatgat tccctcgggc tgtttatcga tccacggaat aagcagctct cctcactgct
     5641 catcggcagc ggtttgccgg gcgggatgat gggaagcctg atggccgacc tgaaaggagc
     5701 ccaaaggagc atcaatacct cgttgaaggg gagaggaata ccggagctga gtgaagatga
     5761 actgatggtc aggatgttca acgaggtgga atacatctgg ccgaagctgg gttatccgcc
     5821 attggttacc cctttcagcc agtatgtcaa gaacatggct atcatgaata ttttgcagat
     5881 ggcaggtgga aaagagaggt acacgatgat cgacaacaat acctgggata tgatcctggg
     5941 taaggccgga aaactgcccg gaccgctggc gccggaaatc gttacgctgg ccggagaaca
     6001 aggaaaggag ttctaccagg gcgtacccca ggacgcattt ccggatgaac tggaaaaata
     6061 caggaaagag atgacggcta ataactggga tacgggtccg gatgaagaag agttgttcga
     6121 gtttgccatg catgacaaac agtacaggga ttttaagtca ggcatagcca aaacccggtt
     6181 tgaggctgaa ctggcaaaag cgaaggagct ggcggagggc ccgaagaaca tgccggctgc
     6241 tccggaacct gcacctgctc ctgctcctgt gaaggaaaag ccggtggaag aaaaaccaaa
     6301 agaggtcgat cccgatatcc gcgagattac tgctcctgcc aaaggccaga tcatttacaa
     6361 tcttttcggg gaggataccg agttttctac cataggcgaa catatatcac cgggcgaccg
     6421 actgtgctat atccagacct cggcctatat tggcgagatc acctgccctt acaacggcat
     6481 tatttcggaa atagtggtcc agcagggagt tcaggttaaa aagggagagg ttatattcag
     6541 gataaaagag gttgttggcg aaataaaacc caaaacgccg aagaaaaagt ctcaaacggc
     6601 aggcaaataa aagactttag acatcttgtt ctggatgatt atcttacaat agccgaagtc
     6661 attgtcagtg agccggcaat ggctttatcg ttgaacaaac ccacctcttc atttccttct
     6721 tttaaagcca ggacatacaa caacggaagg taatgctcag gtgtcgggat ggccatttca
     6781 aattctttac cctgggattt ataatcaatt aatctctga
//
LOCUS       DAOQXS010000085         6797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11579_length_6797_cov_3.655295, whole genome shotgun sequence.
ACCESSION   DAOQXS010000085 DAOQXS010000000
VERSION     DAOQXS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6797_cov_3.655295"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1290
                     /locus_tag="PKM34_03650"
     CDS             <1..>1290
                     /locus_tag="PKM34_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNQ82712.1"
                     /translation="LNETGSESAMDYYQLAYDIFNKEKEYNRRDLATVQSLIGDLYSK
                     KKQYDKGLQYYQSSLITIANQFTDSNFLHNPKLEDLEADIRIFLLLGQKAMGLMKYYQ
                     ENGDLSYLKGAIQTGLLAIDLIDEIRLNVRTENSRMQLSNDINNVYSFIAEGCLLAYD
                     KTGDENWIKLAFTTLERNKSNILLSELSDDNAADIGAVPETTRSLEKEIKSYIYLFTN
                     KIWEEENADSPDVKKLEYYRSTLLTKQKKYDSLMQALQNQYPTYYKYRYDPSVITVDD
                     LQDLLEHDETLIEYAITDLHLMIFLITREDLIAKKVPCPRDLSEKIMAMRNNLDFDKV
                     PEYTIRDYNLFQTTSYELYHLLIEPIRNNIGKDQKLIIIPDGELSYVSFEALIESIFP
                     SDTINFRALPYLIKKHPITYASSATILGLTRKGKAPRL"
     assembly_gap    1291..1300
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1301..2130
                     /locus_tag="PKM34_03655"
     CDS             <1301..2130
                     /locus_tag="PKM34_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNQ82713.1"
                     /translation="ELLLGALPGVRGVLAMAPTASLMTRTLLASNKALADALQFKENL
                     PGAIKESQNILKIMKGRLLLGEEATESAFKKIASQYDILHFATHTDINNINPLSSMLT
                     FNQFGEKQEDGFLQTFEIYNLDLDGELAVLSACSSGNGKLLKGEGVLSLARAFTSAGM
                     PSVVMTLWDVDDISTSLIIQNFYHLLNQGTDKDKALQISKLNYIKLTRSEIQVHPAFW
                     SGSVLYGNSRGFKTGTLNIYICSLCGLTVLLLFTAFIVARKFYRYRKFRNPTSIDLP"
     gene            2117..4513
                     /locus_tag="PKM34_03660"
     CDS             2117..4513
                     /locus_tag="PKM34_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNQ82714.1"
                     /translation="MIYPENYSYKTGFEQIRQLTGHFCLSEMGRERLSRMSFSSDFAL
                     ITRQITETREFLTILETGMPFPTQDYFDLSPALNRIKRPGSYFLPEEMHDLRQSLATI
                     AACLNFFRLPSSQSFTFLIAKAGDLSVPKQVLAETDRIIDEKGRIRDSASPELNRIRR
                     EIDRKTVESEKIIGRMLIAAREAGWTSKDAEVTLSEGRLVIPMLATHKRKIKGIIHDE
                     SASGQTVYLEPETCLEINNEIRRLEGAEKREIISILTQFADFVRPETDELLAAYALLG
                     ELDFIRAKARFAQETGSDKPLLSPEPAFNWMNARHPLLYLSFKQKHKTVVPLNFRLDR
                     EQRILIITGPNAGGKSVCLKTIGLIQYMIQCGMLAPLSPDSQAGIFERIFIDIGDEQS
                     LENDLSTYSSHLLNLNFFMENCNHQSLFLVDELGTGTDPSLGGAIAEASLEKLSQTGA
                     FGIVTTHYSNLKLLAGRIPGIVNGAMLFDTTKLQPLFILATGKPGSSFTFEIARRIGF
                     PEDVIDHAIIKAGKTHLDFERQLQDVEAEKLRLEKKLEEFQVADRFLGEMIEKYEMMK
                     SDLERTRKEILEKAKNDARDILSQSNRMIENTIREIREAQAEKEATKNARIGLEKFRQ
                     KLEKDQEPEKVVETTAKAATGLKKELRAGDMVTIGDKPGTGVIVRIKEGKALLDFSGI
                     RMTVNIHQLKPARKSEQQLSGKVSSFIRDINEKAVNFKLVLDLRGKMADEALQLLQRY
                     IDDAYLLRMKEVSILHGKGEGVLRQVVRNYLSGCDEIISFGDEDADRGGAGITKVSLK
                     "
     gene            4583..4831
                     /locus_tag="PKM34_03665"
     CDS             4583..4831
                     /locus_tag="PKM34_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNQ82715.1"
                     /translation="MKNTLKDKLFRWNSILALAGILTGAIGGYLYYYFVGCRTGTCPL
                     TSNPWLTVVWGATVGYLIFDMFRKKKPAVSGNDKQVDN"
     gene            4971..6518
                     /locus_tag="PKM34_03670"
     CDS             4971..6518
                     /locus_tag="PKM34_03670"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNQ82716.1"
                     /translation="MGLAKKSSDLKKRMRDALRGGGDEAIQKQKATGKLTARERILAL
                     LDPKSFHEYDLFVEHAGKDFDMDKKYLPGDGVITGTGVLSGHPVCISAQDFTIAGGSL
                     GYMHAKKITKIMDHAMKLKVPLIGINDSGGARIQEGVNSLAGYGEIFYRNTQASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKATIGEEISMEDLGGARV
                     HAEKTGNAHFFAESEQECFDQIKQLITFIPWNNQKRADSFPKRPPKSRSFKIDQIIPS
                     DPKQPYDVRDVIKALADDSEFLEIQELWARNIVIGFARIDGETVGCVANQPLYLAGVL
                     DVDSSDKAARFIRYCDAFNIPLLTLVDLPGYLPGIDQEHAGVIRHGAKLLYAYSEATV
                     PKITIILRKAYGGGYIAMCSHHLKADFVFAWPTAEIAVMGPEGAANIIFRNEIKNSDN
                     PEATRKLKIKEYKEKFANPYVAAAYGYIDAVIEPSETRNMLVHALQISMQKQETPPKK
                     KHGIPPF"
     gene            6529..>6797
                     /locus_tag="PKM34_03675"
     CDS             6529..>6797
                     /locus_tag="PKM34_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNQ82717.1"
                     /translation="MTEHKHKQAEETPEQSPYGSMLLDDFAYQTILTDKFRNRKPYEP
                     EDPKKIFAFIPGKIRKVHIKKGSKVKEGEILLVLEAMKMNNNIFSP"
BASE COUNT         2039 a         1516 c         1658 g         1574 t
ORIGIN      
        1 ctgaatgaaa ccgggtcgga aagcgcgatg gattattatc agcttgccta tgatatattt
       61 aacaaagaaa aagaatataa caggcgggat ttggctaccg tacaatcgct cattggtgac
      121 ctttacagta agaagaaaca gtatgacaag ggattgcagt attatcaaag cagcctgatc
      181 actattgcaa accaattcac cgattcgaac tttctccata atccgaaact ggaagatctg
      241 gaagcggata taaggatatt ccttctactg ggccagaaag caatggggct gatgaagtat
      301 taccaggaga acggggacct tagttatctc aaaggcgcta ttcaaacagg gttgcttgcc
      361 atcgacctga tagatgagat aaggttaaac gtgaggactg agaacagcag gatgcaactt
      421 tcaaatgata taaataatgt atattctttt attgctgaag gttgcctgct ggcatatgac
      481 aaaacaggag atgaaaactg gattaaattg gcatttacga ctctggagcg gaataagtcc
      541 aatatccttt tgagcgaatt atcagatgac aatgctgcag atattggggc cgtcccggaa
      601 actacccgat cactggaaaa agagataaag agctatatct atttgtttac taataagata
      661 tgggaggagg aaaatgccga ttcaccggat gtgaaaaaac tggagtatta ccgtagcaca
      721 cttttgacta aacaaaaaaa atacgacagc ctgatgcagg cattacaaaa ccagtatccg
      781 acctattata aatacagata cgatccatcc gtcattacgg tagatgactt gcaggatctg
      841 cttgaacacg atgaaaccct tatagaatat gcgataacag atctccatct aatgatcttc
      901 ctgatcacac gagaagatct tattgctaaa aaagtgccct gtccccgtga tttaagcgag
      961 aagatcatgg ctatgaggaa caacctggat ttcgataagg tcccggagta caccatacgg
     1021 gattataacc ttttccaaac aacatcctat gaattatacc atttacttat tgaaccgatc
     1081 aggaataata tagggaaaga ccaaaaactg attatcattc cagacggtga actgagttat
     1141 gtttcgttcg aagcccttat cgaatccata ttcccgtcgg ataccataaa tttcagggct
     1201 ttgccgtatt taatcaagaa acatcccatt acttatgctt cgtcggcgac cattctcggg
     1261 ctaactcgaa aggggaaggc tccgcgcctt nnnnnnnnnn tcgagttgct gctcggcgcg
     1321 ctgcccgggg tgaggggcgt tctggccatg gcaccgacag caagcctgat gacaaggacg
     1381 cttctggcga gcaacaaagc gctggctgat gccttgcagt tcaaagagaa tcttccgggt
     1441 gcaatcaaag aatcgcaaaa catccttaag ataatgaaag gcagattatt acttggggaa
     1501 gaagcgactg aaagtgcctt taaaaagatc gcatcacaat atgatatctt acatttcgca
     1561 acccatacgg acatcaataa tattaatccc ctttcatcga tgctaacttt taatcaattc
     1621 ggagaaaaac aggaagatgg tttcttacaa acatttgaaa tctataacct ggatctggat
     1681 ggagagcttg ctgtgcttag cgcctgcagc agtggcaacg gaaaactgct gaagggggaa
     1741 ggagtgctca gcctggcaag ggcattcaca tccgccggaa tgcccagcgt ggtgatgacc
     1801 ctgtgggatg tcgatgatat cagtacatcc ctgatcatac agaactttta tcatctattg
     1861 aaccagggca ccgataagga caaagccttg caaatatcaa aactgaacta tatcaaactg
     1921 acacggtcgg aaatccaggt acatcccgcc ttctggtcag gatccgtgtt atatggcaac
     1981 agcaggggat ttaaaaccgg aacactgaat atttacatct gttcgttgtg tggactgact
     2041 gtattacttt tgttcacggc attcattgtg gccaggaaat tctatcggta cagaaaattc
     2101 agaaacccta cgagcattga tttaccctga aaattacagc tataagacag gttttgagca
     2161 gatcaggcag ttaaccggcc atttttgctt atcggaaatg ggcagggaac gattatcccg
     2221 gatgtcgttc agcagcgatt ttgcgctcat aaccaggcaa attacggaaa cccgggaatt
     2281 tctcacgatc ctggaaacag gaatgccctt tcccacacag gactattttg atctttcacc
     2341 ggctctgaac cggatcaaac ggccgggatc gtattttctt ccagaagaaa tgcacgattt
     2401 gcgccagtcg ctggctacga ttgccgcatg ccttaacttt ttccgtctgc cttcatccca
     2461 gtctttcacc ttcctgatag caaaagccgg tgacctgtca gtaccgaaac aagtcctggc
     2521 agaaaccgac aggatcattg atgagaaagg gcggatcagg gatagcgcat ccccggaact
     2581 gaacaggatc aggcgggaga ttgaccggaa aacggtggaa agtgaaaaaa taatcggccg
     2641 catgctaata gcggcgaggg aagccggatg gacaagcaaa gacgcggagg ttacgctgag
     2701 cgaaggccgt ctggtcatcc cgatgctggc aacacacaaa cggaagatca aagggataat
     2761 ccatgatgaa tctgccagcg gtcagactgt ttacctggag ccggagacct gcctggagat
     2821 caacaatgag atccgccggc tggagggggc tgagaaacgt gaaataattt caattctcac
     2881 ccaattcgca gacttcgtcc ggccggaaac cgatgaattg ctagcggcat acgcgttgtt
     2941 aggcgagctt gatttcatcc gggccaaagc ccggtttgcc caggaaaccg gatccgacaa
     3001 gccgctcctg tcgccggagc cggctttcaa ctggatgaat gccaggcacc cgctacttta
     3061 tttatcattt aaacaaaaac ataaaaccgt tgtacccctc aacttccggc tcgacaggga
     3121 acaaaggatt ctcataatta caggccccaa tgccggggga aaatccgtat gtttaaaaac
     3181 cataggactg atccagtata tgatccagtg cgggatgctg gcacctttgt cgcctgattc
     3241 ccaagcagga attttcgaac ggatcttcat tgacataggc gacgaacaat cccttgagaa
     3301 tgacctaagt acatacagct cgcatctgct gaacctgaat ttctttatgg agaactgcaa
     3361 ccatcaaagc ctgttcctgg tcgacgaact gggaacaggc acggatccgt cgctgggagg
     3421 cgctattgcg gaagcttcac tggaaaaact gagccagact ggggcatttg ggattgtcac
     3481 gacacattat tccaatttga aactgctggc cggcaggatc ccgggtattg tcaacggtgc
     3541 tatgctgttc gataccacga aactgcaacc gctgttcata ctggccacag gtaaacccgg
     3601 aagttcattc acttttgaga ttgcccgccg tatcggattt ccggaagatg taatcgatca
     3661 cgcaattata aaagcaggta agacacacct cgattttgag cgccagttgc aggatgttga
     3721 agctgaaaaa cttcgcctgg agaaaaagct ggaagaattc caggtggctg atcgttttct
     3781 tggagaaatg atcgaaaaat atgaaatgat gaaatccgac ctggaacgga ccagaaaaga
     3841 gatcctggag aaagcaaaga acgatgcccg cgacatcctg tcgcaatcca accggatgat
     3901 cgaaaatact atccgtgaga tcagggaagc ccaggctgaa aaagaggcga caaaaaacgc
     3961 caggatcggg cttgaaaaat tcaggcaaaa actggagaag gatcaagagc ctgagaaggt
     4021 cgtggagacc acagcgaaag cagcgaccgg cttaaaaaaa gagttacggg caggcgatat
     4081 ggttaccatc ggggataagc cgggtaccgg ggtgatagtc aggattaaag aggggaaagc
     4141 tttgttggat ttcagtggca tcagaatgac ggtcaatatc catcagctca aacctgcccg
     4201 gaaatcagaa cagcagttat ccggtaaggt cagttccttc atcagggata tcaatgaaaa
     4261 agcggtcaat ttcaagctcg tccttgacct gagagggaaa atggcagacg aggcgcttca
     4321 gctattgcaa cgttacattg atgacgctta tctgctaagg atgaaggaag taagtatttt
     4381 gcacggaaaa ggcgaaggcg ttctccggca ggtggttaga aattaccttt ccggctgtga
     4441 tgagatcatt tcattcgggg atgaggatgc cgaccggggc ggagcgggaa tcacaaaagt
     4501 cagccttaaa taatttgaag attgtggata aaaagtaggt aattttgcat gcaatttttt
     4561 agatataaat aattgcaacc acatgaaaaa tacccttaaa gataaattgt tcaggtggaa
     4621 ctccattttg gcactggctg gaattctcac aggggctatc gggggatacc tttattatta
     4681 ttttgtcggg tgccgcaccg gtacctgccc gcttacctca aatccgtggt taacagtcgt
     4741 atggggtgcc actgtcgggt atcttatctt cgatatgttt cggaagaaaa aacccgctgt
     4801 atcaggaaat gacaaacagg tggataatta accaaagtta cacatctcct taaaaaagat
     4861 tacctttgta tgaccaaaaa acagggtgat gatataatgc attgactatc aagacatacc
     4921 gacaccaaat ttaaataacc aaagactatt gttcactaaa atcagtactt atgggattag
     4981 caaaaaaatc ttcagactta aaaaagagga tgagggatgc tctacgcggc ggcggagatg
     5041 aagctatcca gaaacagaag gctaccggaa agctgacggc aagggaaagg atccttgcct
     5101 tactcgatcc taaatcattt catgaatatg atctatttgt cgagcatgcc ggcaaagatt
     5161 tcgatatgga caagaaatac cttcccggtg atggagtgat tacaggaacg ggagtattat
     5221 ccgggcatcc ggtgtgcatt tctgcgcagg actttaccat agcaggcggt tcgctgggtt
     5281 acatgcatgc aaaaaagatc accaagatca tggaccatgc catgaagctg aaagtgccgc
     5341 tgatcggcat caacgattca ggcggagccc gaatccagga aggggtgaat tcgctggccg
     5401 gatatggaga aatattttac cggaacacac aagcttcagg cgtgatcccg cagatttcag
     5461 tcattctcgg gccatgtgcc ggaggggcgg tatattcgcc ggccctgacc gattttgtct
     5521 ttgtcgtgga taagatatcc aagatgttca tcaccggccc ggaagtcatc aaagccacga
     5581 ttggagaaga aatatcgatg gaggatctcg gcggtgccag ggtccatgcg gaaaagacag
     5641 gtaatgccca cttttttgcc gagagcgaac aggagtgttt cgaccagatc aagcaactca
     5701 tcacctttat tccatggaat aaccagaaac gggccgattc cttccccaaa aggccgccca
     5761 agagccggag cttcaaaatc gaccagatca tcccttccga tccaaagcaa ccgtatgatg
     5821 tccgcgatgt gatcaaagcc ctggcggatg attcggagtt ccttgagatc caggagctat
     5881 gggccaggaa tatcgttatc gggtttgcac gcatcgatgg cgaaacggtc ggatgcgtgg
     5941 ccaaccagcc gttatacctc gccggggtgc ttgatgttga ctcttcggat aaagcggccc
     6001 ggtttatccg gtactgtgac gcctttaata ttcctttgct tactctggtt gacctcccgg
     6061 gatatttgcc cggaatagac caggagcatg cgggcgtcat ccggcatggt gcaaagttgc
     6121 tgtatgccta ttcggaagcc accgtaccca aaatcacgat aatactcaga aaggcatacg
     6181 gaggaggata tatcgccatg tgctcccacc atcttaaagc cgactttgtg tttgcgtggc
     6241 caacggccga aattgccgtc atgggcccgg aaggggcggc aaatatcatt ttcaggaacg
     6301 agatcaaaaa ctccgataat ccggaagcga cccgtaaact gaagatcaag gagtacaagg
     6361 agaagtttgc caatccatat gttgctgcgg cgtatggtta cattgatgcc gtcattgaac
     6421 catccgaaac acggaatatg ctggtgcacg ccttgcaaat ctccatgcag aaacaagaga
     6481 ctccgcccaa gaaaaagcac ggtattccac cattctaaac cgacaggcat gacagaacat
     6541 aagcacaagc aggctgagga gacacctgaa cagtctccat acggttctat gcttctggat
     6601 gactttgcat accagactat attaacggat aagttccgga accgaaaacc ctatgaaccc
     6661 gaagacccga aaaagatctt cgcctttatc ccgggaaaaa tcaggaaagt acatatcaaa
     6721 aaagggagca aggtaaagga aggggaaatt ctcctggtac tggaagcgat gaaaatgaac
     6781 aataatattt tttcacc
//
LOCUS       DAOQXS010000086         6752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11676_length_6752_cov_4.118411, whole genome shotgun sequence.
ACCESSION   DAOQXS010000086 DAOQXS010000000
VERSION     DAOQXS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11676_length_6752_cov_4.118411"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKM34_03680"
     CDS             complement(<1..188)
                     /locus_tag="PKM34_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82718.1"
                     /translation="MYKELLSSLIFGQLQSYGSIAAFPLSIPLKPGPFDYLTLSEALS
                     MLLIRIEEVSESGSVPEL"
     gene            391..1584
                     /locus_tag="PKM34_03685"
     CDS             391..1584
                     /locus_tag="PKM34_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ82719.1"
                     /translation="MERLIEKSNKAILSTDYPYRRHVIGEINWNWKMNGIIGARGTGK
                     TTLLLQKLKELKQEGHDVLYARLDDFYFSDHRLNDLADEFRKQGGVFLYLDEIHKYPG
                     WPEELKNIYDTMPELKVVFSGSSIIDLMATDADLSRRALNYEMTGLSFREYLLMSGIS
                     NSTPFSLDEILQKHVEIAAGMIRQFPVLKYFRTYLRSGYYPYFLEPDRDYLLTLEQVI
                     RTVMEVDMRFIENYDVTQSRKMLLLLKIIASSAPFKPNVLKISERTGLHRHTVLQYFH
                     YLEKARMIKLLNHPEKHISRLQKPDKIFLENPNLFYALNPEMVDIGNLRETFVLNQLS
                     VNHEVNLHARVDFMVDFKYLLEIGGKNKKSSQIKDLDHAFLFMDDIEIGNKRTIPIWL
                     LGFLY"
     gene            complement(1731..2018)
                     /locus_tag="PKM34_03690"
     CDS             complement(1731..2018)
                     /locus_tag="PKM34_03690"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ82720.1"
                     /translation="MISKITSLLAKNQAVNRLVLFGSRAKGNFKPGSDIDIAVFGHNL
                     TLDDFLVFSINIDQLELANKVDLVHYEKIKEKELIEHIARVGIVLYEKAEE"
     gene            complement(2077..2490)
                     /locus_tag="PKM34_03695"
     CDS             complement(2077..2490)
                     /locus_tag="PKM34_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNQ82721.1"
                     /translation="MGDKDIRWLQRFSNLKKAFMKLKEAVEQEPLSELENEGLIQRFE
                     YTYELAWKTLEDLLEEKGYLDIKGPAPVIRQAFQDGYIADGDGWMRLKKSRENTSHTY
                     NKEIAKEIVEQIISFYFFLFEDLILKLEKEEQSKV"
     gene            complement(2670..2858)
                     /locus_tag="PKM34_03700"
     CDS             complement(2670..2858)
                     /locus_tag="PKM34_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82722.1"
                     /translation="MINISLPGLYGVKEVSLADINGRIVEKMSFSHQGKAADMIQIST
                     ASLPAGNISSGSPPTIRH"
     gene            complement(2855..3073)
                     /locus_tag="PKM34_03705"
     CDS             complement(2855..3073)
                     /locus_tag="PKM34_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82723.1"
                     /translation="MKSFFIFWMTAILIVSSLSVNSQWSCDPANPVVVCNQAGLQNNV
                     MTIADNNGGVYVYWLDARLNPGSPAREI"
     gene            3824..6250
                     /locus_tag="PKM34_03710"
     CDS             3824..6250
                     /locus_tag="PKM34_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNQ82724.1"
                     /translation="MKKNRIYTGIIAMMLIALASQVFSQARVQVIHNSADAAAEVVDV
                     WLNDQLLIDNFKFRTATPFIDAPAGEKFDVTIQPANSTDTTNGLARFTYQLENNKRYI
                     LVANGIVIPTGYNPATPFNIYVWDLGREAAKLPSNTDVLVFHGSTDAPIVDVVEVAVP
                     AGTVVNDLEYAEFAGYLELPTADYSLQIRDITGTTTVAEFAAPLAALGLQGSALAVVA
                     SGFLNPGNNNDGPAFGLWVALPTGGNLIPLPNVPISTARVQLIHNSADIAAQTVDIWL
                     ENELMIDNFSFRTATPFVDVPANKDLNVTIAPANSNGFSDGLASFTLNLEGGNKYLLV
                     ANGIVSANGYSPATPFSIFSYETAREMAMMEGNTDVLVFHGSTDAPTVDIVETNFGIG
                     TIVDNLSYGEFAGYLEVPSSDYTFQLRDANGKVTVATFEAPLFSYGLQNEAVTVLASG
                     FLNPQENNDGAAFGLWVALPQGGDLVPLPQVIPVTQTARVQVIHNSADAAAEFVDVWL
                     NDQLLIDNFKFRTASPFIDAPAGTDFDVTIAPSNSVSVSEGLATFTYNLAEGNTYILV
                     ANGIVVPQGYNPPTPFDIYVYDMGREMASNPANTDVLVFHGSTDAPVVDVVEVGVGAG
                     TIIDNLAYADFAGYLELPTADYQIDIRDESGTVTVVSYSAPLASFGLQGRSIAVVASG
                     FLNPGANNDGAAFGLYVALPSGGELIALDPAIPTDVTENRTDEIKASIFPNPARDNMN
                     VQFELPQASDVQVRIYDLTGTMVMSRQFSASQAASQQRIDIGNLPGGLYFVKTQAGNS
                     SFVTRLTKVN"
     gene            complement(6323..>6752)
                     /locus_tag="PKM34_03715"
     CDS             complement(6323..>6752)
                     /locus_tag="PKM34_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82725.1"
                     /translation="IVSKLYGSQYIHRRLVHLGFESELITLRHAGHEPQYEPGKYRAV
                     MDTIISRSTDFYFRSLLHFPDIAGPRQVAIGMSPSSYSVPVQEGVSYYWQATGGKVLT
                     GSFKNEAKVVWLAKEKGMVSVVLYHRNGANAEVRLPVVLP"
BASE COUNT         1788 a         1593 c         1588 g         1783 t
ORIGIN      
        1 ttcagttcgg ggacggagcc gctctcgctg acctcttcga tccggatcag cagcatggat
       61 aacgcttcgg acaaggtgag ataatcgaac ggtccgggtt tcagggggat cgatagcggg
      121 aatgcagcga tggaaccata ggattgaagc tggccgaaga tgagtgatga taataattct
      181 ttgtacatgg tcggttaatt tgccgacaaa ggaaaaaagg ggaggtgcag tgtatttgca
      241 tttgctgaaa ggattttttt ggtggagaaa gaaggcttag tcccgtaggg acgccagtat
      301 ggaagaccgg gaacaggaat taaatatttc aatattggtt attataatca ccaaaataga
      361 attatatttg ttgaatcgaa tcatcagaat atggaacggc ttattgaaaa atcaaataaa
      421 gcgatccttt ccaccgatta tccttatcgt cgtcatgtga ttggtgagat caactggaac
      481 tggaaaatga atggaatcat cggtgcaagg gggacgggaa agacgacgct attgctccag
      541 aaactgaaag agctcaagca agaaggtcat gatgttctct acgccagatt ggatgatttt
      601 tatttctccg atcaccggct gaatgatctg gctgatgagt tcaggaaaca aggcggcgtg
      661 tttctttacc tggatgaaat tcataagtat cccgggtggc cggaagaact gaaaaatatt
      721 tacgatacca tgccagaact taaggtcgta ttttccggtt cttccattat cgatctgatg
      781 gcaacagatg ccgacctgag ccggagagcg ctgaattacg aaatgaccgg cctttctttc
      841 cgtgaatatc tcctcatgtc aggcatctcg aattccactc ctttttccct ggatgaaata
      901 ctgcagaagc atgtcgaaat cgctgccgga atgatcaggc agtttcctgt tctgaaatat
      961 ttccgtacat acctgagaag cggatactat ccgtattttc tcgaaccgga ccgcgattac
     1021 ctgcttaccc tcgaacaggt gatccggacg gtcatggaag ttgatatgcg gtttatagag
     1081 aattacgatg ttacccagag ccggaaaatg cttttgctat tgaagataat cgcttcttct
     1141 gcgcctttca aacccaatgt attgaaaata agtgaaagga ccggcctgca ccgacatact
     1201 gtgctgcaat atttccatta cctcgaaaaa gccaggatga tcaaattgct gaatcatccg
     1261 gaaaagcata taagccggct gcagaaaccg gataaaatct ttctcgaaaa ccccaacctg
     1321 ttctatgccc tgaatccgga aatggtcgat attggaaatc tcagggagac gtttgtcctg
     1381 aaccagttat ctgtaaatca tgaagtgaat ctgcacgccc gggttgattt tatggtggat
     1441 tttaaatatc tgttagagat cggagggaaa aataagaaaa gtagccagat caaggatctt
     1501 gatcatgctt tcctgttcat ggatgatatc gaaatcggca acaaaaggac cattccaatt
     1561 tggttactgg gattcttgta ttgatatacc aatgtccctg ggaaaagaca ggagacagaa
     1621 ttcaccgcag attacacaga tttacaccga gttaacacag agacccatca ctcacccccc
     1681 cccctttgtc ctgtagggac gatagtatgg tagctaaaat cacaggtcat tcattcttca
     1741 gctttttcat aaagcacaat cccgacacgc gcgatatgtt cgataagttc cttttccttg
     1801 attttctcgt aatgaacaag gtctacctta ttggccagtt caagttgatc gatatttatt
     1861 gaaaaaacca ggaaatcgtc gagtgttaaa ttatgaccaa aaaccgctat gtcaatatct
     1921 gaaccgggct tgaaatttcc tttcgccctg gagccgaata atacgagcct gtttacagcc
     1981 tgattttttg ccaacaggga agtgattttg gaaatcatat catttttaag cccgtatggc
     2041 ataaagcaga cttttagtat taattgcgat caatgtctat accttacttt gttcttcctt
     2101 ttccagtttg aggatcagat cttcgaacag aaagaaatag aatgaaataa tttgctcaac
     2161 aatctctttg gcgatttctt tattataggt atgcgatgtg ttttcacggc ttttctttaa
     2221 ccgcatccag ccatcaccat cagcaatata accatcctgg aacgcctgcc tgatcaccgg
     2281 tgcgggtcct ttaatatcca gatatccttt ttcttccaaa agatcctcca acgtcttcca
     2341 ggcaagctcg taggtatatt caaaccgttg tattagccct tcgttctcaa gttctgataa
     2401 cggttcctgc tcaaccgctt ctttaagctt cataaaggct ttcttcagat tgctgaaccg
     2461 ctgcagccac cgtatgtctt tatcacccat aatcaataat tattgaggat caacaaatat
     2521 agtgaatgtc ggggagaata gggcgggtaa aagatgtgaa atgctttaag gctggaaaat
     2581 cattcaccac agatctacac aacttgtccc gcaaggcggg agagttaaca cagagtgaac
     2641 tcttccttac ctgacgatga ccaggtgttt cagtgtctga tcgtgggtgg cgacccggag
     2701 gaaatattgc cagcggggag cgatgcggtt gatatctgta tcatatccgc cgctttgccc
     2761 tggtggctga acgacatctt ctccactatc cttccattta tatctgccag tgaaacctct
     2821 ttcacgccat acaggcccgg cagggaaatg ttgatcatat ctcacgggca ggtgagccgg
     2881 gattcagccg ggcatcgagc cagtaaacat aaacaccgcc gttgttgtcg gctatggtca
     2941 tgacattgtt ttgcagaccg gcctggttgc acacaactac cgggtttgcc ggatcacacg
     3001 accattgtga attgactgaa agggatgata caattagaat agcagtcatc caaaatataa
     3061 agaatgattt catggtattg cgatttagtg taagcaggaa aaattggatt gcggcaaaat
     3121 tacatgcagg gaaaggatac cggtggattt taatattaga ttaacaaatt tgggccatga
     3181 atctttattc agcagagagt ttacgtagga ataggaggtt gagtaaaata ttagatgctt
     3241 aatgttgagt cccatccgaa aagtgtattt acccacccct aacccgaggc tgtctcaaaa
     3301 gccttattta tcgaattgct ctcaattatt tgttcgtgct gaggctgtat caaaagtaat
     3361 aaaccacaaa gggacaccaa ggatttcacg aaggaacaca aagtttatta tttgataacc
     3421 agatacttag tgcgactttg tgatgtactt tgtgcgactt tgtggttgaa tattagactt
     3481 ttgagacatc cccggaaata tccaattaat ccagaatgct gatattaaat taattacata
     3541 gattgaagct atacccctcc ctttataagg gaggagcagg ggtgggtact tttggataga
     3601 gatttctatt cggagtagac tcaatgctta ttgagttatg tttaatgatt atgacaaaat
     3661 atattaaagg tattatgttt ttaaaattca taaaaagtta taaaacaatg atataagaaa
     3721 atatattgaa tataatgttt aagaaaaaca ttaaataaat gaacaaaaat actaaacctg
     3781 ctgtttagct ttcatgaaat ttaaccctat aaaattaaac attatgaaaa agaacagaat
     3841 ttacaccgga attatagcca tgatgcttat tgcactggca tcacaggtct tctctcaggc
     3901 cagggtacag gttatccata attcagccga tgccgccgcg gaggtggtcg atgtctggct
     3961 gaacgaccag ttactgatcg acaatttcaa gttccgcacg gcaaccccgt ttatcgacgc
     4021 cccggcggga gagaaattcg atgtcaccat ccagccggcc aactctaccg ataccaccaa
     4081 cggactggcc cgttttactt accagcttga gaacaacaag cgttatatcc tcgtcgctaa
     4141 cggcattgtg atccctaccg gttacaatcc ggccacacca ttcaatattt atgtctggga
     4201 cctgggacgc gaggcggcta aacttccctc caataccgat gtcctggtct tccatggctc
     4261 gaccgatgca ccgattgtgg atgtggtaga agtggccgtt cccgcaggta ccgtggtgaa
     4321 cgacctggaa tatgccgaat ttgccggtta cctggaattg ccgacagccg actattcgct
     4381 gcagatcagg gatattacgg gcaccactac cgttgctgaa ttcgccgctc cgctggctgc
     4441 cctcggatta cagggcagtg cactggccgt cgtggcatcg ggcttcctga acccgggcaa
     4501 taacaatgat ggccccgctt tcgggctctg ggtcgctttg ccgaccggcg gcaacctgat
     4561 cccgcttcca aacgttccga tctccactgc aagggtgcag ttgatccaca actccgccga
     4621 tatcgcggct caaacggtcg atatatggct tgaaaacgaa ctgatgatcg acaatttcag
     4681 tttccgcacc gccactccgt tcgtggatgt gccggccaat aaagatctga atgtgaccat
     4741 agcaccggct aactccaatg gtttctccga tggcctggcc agctttactt taaacctgga
     4801 aggcgggaac aaatatttgc tcgtcgccaa cggtatcgtt tcagccaatg gttacagccc
     4861 tgcaacgcca ttcagcattt tctcctatga aacggcccgt gaaatggcta tgatggaagg
     4921 caacaccgat gttctcgtgt tccacggatc cactgatgcc ccgacggtcg acatcgtcga
     4981 gaccaacttc ggaatcggca ccatcgtcga caacctttct tacggtgaat ttgccggcta
     5041 tctcgaagtg ccttcttccg actatacttt ccagcttcgt gatgcgaacg gaaaagtgac
     5101 cgttgccact ttcgaagctc cgctattctc atacggtctt cagaatgaag ctgtaaccgt
     5161 tcttgcttcc ggattcttaa atccgcagga aaacaacgat ggagcggcat tcggactatg
     5221 ggttgcgctg ccgcagggcg gtgaccttgt accgctcccg caggtaattc ctgtcacaca
     5281 gaccgcgcgg gtccaggtga tccataactc ggccgatgcc gctgctgaat tcgttgatgt
     5341 atggctgaac gaccagttgc tgatcgacaa cttcaagttc cgcaccgcca gtccgttcat
     5401 cgacgccccg gccggaaccg attttgatgt cacgattgcc ccttccaact cggtgagcgt
     5461 ttccgaaggg ctggccacat tcacctacaa cctggctgaa ggcaatactt atattctggt
     5521 cgctaacgga attgtggtcc cccagggata taatcctcct acgccattcg atatttatgt
     5581 ttacgatatg ggccgcgaaa tggcctccaa tccggccaat accgatgtgc tggtattcca
     5641 cggctcaacc gatgcaccgg ttgtagatgt ggtggaggtt ggcgttggcg ccggcactat
     5701 cattgataac ctggcctatg ccgatttcgc cggttacctc gaactaccta cggccgacta
     5761 ccagattgac atcagggacg aaagcggcac agtgacggtc gtttcctaca gcgctccgct
     5821 cgcttcattc ggactccagg ggcggtcgat cgcagtcgtt gcttccggtt tcctcaatcc
     5881 tggcgcgaac aacgacggag ccgctttcgg tctctatgtt gcgttgcctt caggcggtga
     5941 gctgatagcc cttgaccctg cgattccgac cgatgttacg gagaacagga cagatgagat
     6001 taaagcttcc atcttcccga atccggcacg tgataatatg aatgttcagt ttgagctgcc
     6061 gcaggcttcc gatgtgcagg ttcggatcta cgacctgacc ggtaccatgg tgatgagccg
     6121 ccagttcagc gcttcgcagg cagccagcca gcagcggatc gacattggca acctgcccgg
     6181 cggattgtac tttgtcaaga cccaggccgg taattcaagc tttgtgacca ggctgacgaa
     6241 ggtaaattaa gatagtggtt tgttgagaac catatattta gttaataatg cgaaggctgc
     6301 ctcattcaag ggcagccttt tttcatggta agaccaccgg cagccggacc tctgcgttgg
     6361 cgccgttgcg gtgatataaa acgaccgata ccattccctt ctctttagcc agccagacca
     6421 cttttgcctc gtttttaaag ctacctgtca agacctttcc accggtggcc tgccagtaat
     6481 acgacacgcc ctcctgcacc ggaacggaat aggatgatgg cgacatgccg atggctacct
     6541 gccgcgggcc ggcgatatcg gggaaatgga gaagtgaacg gaagtaaaag tcggtggagc
     6601 gggaaatgat cgtatccatg accgcccggt actttccagg ctcgtattgc ggttcgtggc
     6661 cggcatggcg gagggttatg agttcgctct caaatcccag atgaacaagt cgccggtgaa
     6721 tgtattgcga accgtacagc ttgctcacaa tg
//
LOCUS       DAOQXS010000087         6732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11719_length_6732_cov_3.636064, whole genome shotgun sequence.
ACCESSION   DAOQXS010000087 DAOQXS010000000
VERSION     DAOQXS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11719_length_6732_cov_3.636064"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKM34_03720"
     CDS             <1..193
                     /locus_tag="PKM34_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ82726.1"
                     /translation="LENPVELAEFLENKSIEIKVPFSQKTVNYNPARYIGVGVSRLGT
                     SRAVSVGAYAYALAELDKI"
     gene            359..1189
                     /locus_tag="PKM34_03725"
     CDS             359..1189
                     /locus_tag="PKM34_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ82727.1"
                     /translation="MKLYSIETGNLKLDGGAMFGVVPKSLWSKVYPADENNLCNWAMR
                     CLLVVDEDRRILIDNGIGDKQDWNFLRHYHLNGDDTLEKSLAKAGFGKEDITDVILTH
                     LHFDHCGGSVEWNHDRTGYKLAFPNANYWVSRQQYNWAVEPNHRESASYLKENILPIE
                     ESGKLKLIEGEGEILPNITIKLYHGHTDGQAIPHIRYNGKTVVFMADLLPSTAHVPMP
                     WIMGYDTRPLITLEDKTRFYREALENDYILFLEHDLYHECCTLHQTDKGVKVKETFEL
                     "
     gene            complement(1197..>1554)
                     /locus_tag="PKM34_03730"
     CDS             complement(1197..>1554)
                     /locus_tag="PKM34_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ82728.1"
                     /translation="NEISFKLAGRTSFSMAFKEASPKILEPIYDVEVFVPADKMGGVM
                     TDLQGRRAIIMGMDGGTIRARVPLAELNRYSTALSSLTSGRASYTMKFNEYAPVPSDI
                     QEKLLKEYEESAKDED"
     assembly_gap    1555..1564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1565..3406)
                     /locus_tag="PKM34_03735"
     CDS             complement(<1565..3406)
                     /locus_tag="PKM34_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ82729.1"
                     /translation="MKVFQTNEIRNIALIGGAKTGKTTLAEDMLFEGGVINRRGSIED
                     KNTVSDYRDIEMERQNSVYSSVLYSIYNNHKINVIDTPGFDDFVGEVISAMKVVDAAV
                     SVVNAQNGVEPGTETTWRLCTKELMPVIFAINHLEHDNSNFDDTVSQLKSSFSSAVTV
                     VQYPLHTGHGFNSVIDLLKMKMLKYPDGGGKPEILDIPANEKGKAEELQAKLIEDLAA
                     NDESLMESFFENGTLSEEEISRGLRLGLRNRGIFPVLCVNAKQNMGVGRLLEFIINNV
                     SAPNEMPPVKTVSGKEVKCLATEPAMAFVFKTTIEQHLGEVSFFKVFSGEIHEAEDMI
                     NGTNATKERLSQVFVFAGKNRDKVEKMVAGDIGATIKLKNTLTNHTLNAPKFADEKIA
                     PIEYPDSKFRVACKAVNQADDEKLGMALNELHKMDQTIIAGYKKELRQLILEGQGELH
                     LNIAKWHLENNIKIQVNYLPPRIPYRETITRVAQATYQHKKQSGGAGQFGEVHMVIEP
                     HEEGKPDPSSVKLKQMMRQSSVTPLTISKGDTEISLSIRGKDEIQLEWGGKLVFYNCI
                     VGGSIDTRFLPAILKGLMEKMEQGPLTGSYARDIRVYVYDVKMHPVDS"
     gene            3605..>6732
                     /locus_tag="PKM34_03740"
     CDS             3605..>6732
                     /locus_tag="PKM34_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82730.1"
                     /translation="MIRKALPFLVILFFSITLAGQEIPEAYRNYNAKYPSGPVMTNRD
                     AALLRSLPEKKVPETAVRDQLPAIVDNSTLPYLRPVFLQEGPSCGQAAMVGYNFTYEM
                     ACKRDQPALFPQTQYPTHFTWNFQNGGDGWYGVSYFHSVEILRMCGCMNSYDYGGFFD
                     DGRRWINGYNLYNNGMYNRVRQMYRINTSTEEGILALKHWLYNHMGEQEHGGVASYYA
                     NVPWNAVFLNDTTPEGGKYVVTWWYPLASHAMTIVGYNDSIRYDYNGDGQYTNTIDLN
                     NDQVIDPRDWEFGGVKFVNSHGTGSQDSGFCYMMYKVLAEKFETGGIWNQEVHILDVD
                     EDYLPLMAFKVKIKHTNRGKIKVMAGVSQDTSDISPAWMMDFPMINYQGGNHFMQGND
                     TLESLKTLEFGLDVTPLLGHLDSGLPAKFFLVVDEDDPEYQGDGQILSFSVMDYTGSA
                     QEFISDDIPVDIENNSISMASVMFSPDFEIITITTDSLPPFVVDEPYSFQLGAAGGRI
                     PYSWEIAGQYTVEQSTATMPEVDEYRVLESAEDDTIMAVALGFDFPFYGVMYDTVFMH
                     INGHLQFTGDQIPWPYMMEPELMFRSIPMIAPLSYAGLTIMPAEGDGGWVETSDTAAI
                     FRWKLSAEGNLGTTDINFTVKLEDDGTIGFIYGPSTLGGFGWLAGLSAGNKTDFLVSP
                     YSGQPQITPGTSIKFSYQSLPGTFQLSENGLLSGTPHSDDRIHDVAVRVTDRSGISVA
                     RDFQLTSGPYIYLSVMPGNDDQVFYGDTVSLQLTLVNGGTEILENPFISLSTSDPFII
                     FLDSQCQTGTIPAGESVSVTDAFSFVVSTFIPDHSDIQFETVLDASGRNWVKDLVLQS
                     NAPGLFLERNEIGDEDNRLSPGETAPLLATLRNSGHAAIDQVMSELVSLSPEVVVSGN
                     TVQDFGSIGKGASVTRSFTLTADDSTPSGTPVTLVLTTTSASGIGQQDTIEMKVGKTP
                     VMVIDMAVDKNSGPVIFSLLNELNVLSEYDYSITPKIFDYESLFICLGYYYQNHLLTL
                     GEGTLIANYLDAG"
BASE COUNT         1649 a         1727 c         1645 g         1701 t
ORIGIN      
        1 cctggaaaac ccggtggaac tggccgagtt cctggaaaac aagtcgatcg aaatcaaagt
       61 gccgttcagc caaaaaaccg tcaattataa cccggcacgg tatatcggcg tcggcgtttc
      121 ccggctgggc acatccaggg ccgtatcggt cggcgcctac gcctatgcac tggctgaact
      181 ggataagatt tagttagagg ctattcgtgt caattcgcct cattcgtgtt gattcgtgtt
      241 ccctttggaa taacggatga gacgaatttt acgaataacc acggataaat aatagcagaa
      301 atattaacta ttccggattt tatttgttat tagcttcaga aataaaaatc cattgattat
      361 gaaactctac agcatcgaaa ccggaaacct gaaactcgac ggcggcgcca tgttcggcgt
      421 cgtacccaaa tcactctggt cgaaagttta tcctgccgat gagaacaatc tgtgcaattg
      481 ggccatgcgc tgcctgctgg tggtcgatga agaccgcagg atcctgatcg acaacggaat
      541 cggcgacaag caggactgga attttctccg ccattatcat ctcaacggag atgacacgct
      601 ggagaagtcg ctggcaaaag ccggtttcgg taaggaggat attacggatg tgatcctgac
      661 gcacctgcat ttcgaccatt gcggcggaag tgtcgaatgg aaccacgacc gcaccgggta
      721 caagctggct tttcccaatg ccaattactg ggtcagccgt cagcagtaca actgggccgt
      781 tgagcccaat catcgcgaaa gcgcttctta tctgaaagaa aacattttac ccattgaaga
      841 gagcggaaag ctgaaactaa ttgaaggcga aggtgaaatt cttcccaaca tcaccataaa
      901 actttaccat ggccatacag acggacaggc gatacctcac attcgataca acggtaaaac
      961 tgtggtattt atggccgatc tgcttccctc aacagcccat gtgccgatgc cgtggatcat
     1021 gggttacgat acccgcccgt tgattacttt agaagacaaa acccgctttt acagggaagc
     1081 gctggaaaac gactacatcc tgttcctgga acatgatctt taccatgaat gctgtaccct
     1141 tcaccagacc gacaaaggcg ttaaagtaaa ggaaaccttt gaactttgaa ctaattctag
     1201 tcttcgtctt tcgcgctttc ttcgtattcc ttcagcagtt tctcctggat atcggacggt
     1261 accggggcat attcgttgaa tttcatcgtg tagcttgcac ggccgctggt gagcgagctt
     1321 aaagccgtcg aatagcggtt cagttcggca agcggcacgc gggcacggat cgttccgcca
     1381 tccataccca taatgatggc acgccttcct tgcaggtcgg tcatgacgcc acccatttta
     1441 tcggccggga cgaagacttc gacatcatag atcggctcga ggatcttcgg gctggcttcc
     1501 ttgaaagcca tgctgaagga ggtacggccg gcgagtttga aggagatttc attgnnnnnn
     1561 nnnntgaatc gaccgggtgc atcttgacat cataaacata gacgcggata tcgcgggcat
     1621 aggaaccggt aagcgggccc tgctccattt tctccatcaa tcccttgagg atagcgggaa
     1681 ggaaacgggt atcgatcgaa ccgccgacga tgcagttata gaaaacgagt tttccgcccc
     1741 attcgagctg gatctcgtct ttaccgcgga tcgacagtga aatttcggta tcgcctttgg
     1801 aaatagtcag cggggtgacc gacgactggc gcatcatttg tttcagcttg acgctcgacg
     1861 ggtcgggttt gccctcttcg tgcggctcga tgaccatgtg cacttcgcca aactggccgg
     1921 cgccgcccga ctgcttctta tgctggtagg tagcctgggc aactcttgtg atcgtttccc
     1981 tgtaaggaat acgcggagga aggtagttca cctgtatctt gatattgttt tccaggtgcc
     2041 acttggcaat gttcaggtga agttcgccct ggccctccag gatcagctgg cgcaattcct
     2101 tcttgtaacc ggcaatgatc gtctggtcca tcttgtgcaa ctcattcagc gccatgccca
     2161 gtttttcgtc atcggcctga ttgacggctt tacaagccac gcggaacttc gaatcgggat
     2221 actcgatcgg ggcgatcttc tcatccgcaa acttgggagc attcagcgta tggttggtca
     2281 gcgtattctt cagcttgatg gtagcgccaa tatcaccggc gaccatcttt tccaccttgt
     2341 cgcggttctt gccggcaaag acgaacacct gcgagagacg ctccttggtg gcattggtgc
     2401 cgttgatcat atcttctgct tcatggatct caccgctgaa aaccttgaaa aagctcacct
     2461 cacccaggtg ctgctcgatg gtcgttttga agacaaaagc catagccggc tcggtagcca
     2521 gacatttgac ctcttttccc gagacagtct tgacaggcgg catttcattc ggggcggaaa
     2581 cattgttgat gatgaattcc agcaaccggc ccacgcccat attctgtttg gcattgacac
     2641 aaagcaccgg gaaaatgccc ctgttgcgca aacccaggcg cagcccgcgg gagatttcct
     2701 cttccgacaa agtcccgttt tcgaagaacg attccatcag cgattcatcg ttggctgcca
     2761 gatcttcgat cagctttgcc tgcaattctt ccgctttgcc tttttcattg gcaggaatat
     2821 ccagaatctc cggttttccc cctccatccg ggtatttcag cattttcatc ttaagaaggt
     2881 cgatgaccga attaaaacca tgtccggtgt gaagcggata ctggaccacc gtaaccgcgc
     2941 tgctgaagga gctcttgagc tggctgacgg tatcgtcgaa attcgagttg tcgtgttcca
     3001 ggtggttgat ggcaaagatc accggcatga gttctttggt acaaagcctc caggttgtct
     3061 ccgttcccgg ttcaacgcca ttctgtgcat tgaccacgga tacggctgca tccaccactt
     3121 tcatcgcgga aattacctct cccacaaagt catcaaaacc cggcgtatcg ataacattga
     3181 tcttgtgatt gttataaatg gaatacaaaa cggatgaata gactgaattc tgtctttcca
     3241 tttcaatatc acggtaatcg gaaaccgtat ttttatcttc gatcgatccc ctcctgttaa
     3301 ttacgccgcc ttcgaacaac atatcttctg ccaatgttgt tttacccgtc ttggcgccgc
     3361 ctatcaacgc gatgttcctg atctcgttcg tttgaaatac cttcattttc tgtaatttat
     3421 aattgttaac ctgttaattc cgaataaggg acgcaaaagt agaaaaaagc tgccaacttt
     3481 caaagaagcc gctgaaaaaa gtcttccata accttaactt gctgaaagtt cccgataact
     3541 ttttaactat ttacacattt aaaaaataat tttactttta tttcaagtct taaaacccat
     3601 tttaatgata aggaaagcgc taccattcct ggtaatattg tttttctcca taaccctggc
     3661 cggacaagaa attcccgagg cataccggaa ttataatgcg aaatatccgt cggggccggt
     3721 tatgaccaac cgggatgcgg ctcttcttcg ctccctgcca gaaaaaaagg ttccggaaac
     3781 cgcagtgagg gaccagcttc cggcaattgt cgataattca acccttcctt acctgaggcc
     3841 ggtatttctg caggagggcc ccagttgcgg acaggcagcc atggtcggtt ataatttcac
     3901 atacgaaatg gcttgcaaga gagaccagcc cgccttgttc ccgcaaaccc agtatccgac
     3961 ccatttcacc tggaattttc agaacggtgg cgatggctgg tatggagtca gctatttcca
     4021 cagtgtcgaa atcctccgca tgtgcgggtg tatgaattct tacgactatg gcggtttttt
     4081 cgatgacggg cgccgctgga ttaacggtta caacctgtat aacaatggta tgtacaacag
     4141 ggtcaggcag atgtaccgga tcaacacctc gacggaagaa ggaatcctgg cactgaagca
     4201 ctggctctac aatcatatgg gagaacagga acatggcggc gttgcgagct actacgccaa
     4261 tgtcccctgg aatgcagttt tcctcaatga tacgactccc gaaggaggca aatacgtagt
     4321 gacctggtgg tacccgctgg cttcccacgc catgaccatt gtgggttaca atgattctat
     4381 ccgctacgat tacaacggcg acgggcaata caccaatact atcgatctta acaacgacca
     4441 ggtcatcgac cccagggact gggagttcgg cggagtaaag tttgtcaaca gccacggaac
     4501 cgggtcgcag gacagtggtt tttgttacat gatgtacaag gttctggccg aaaagtttga
     4561 aacaggcggg atctggaacc aggaggttca tatcctggat gttgatgaag attatttgcc
     4621 cctgatggcg tttaaagtaa aaataaaaca tactaaccgg gggaagatta aagtgatggc
     4681 aggtgtttca caggatacat cggatatttc accggcatgg atgatggatt ttccgatgat
     4741 caactaccag ggcggcaacc acttcatgca ggggaacgat acgctggaat cgctcaaaac
     4801 ccttgagttc ggcctggatg ttaccccgct gctcggtcac ctggattcgg gcctgccggc
     4861 aaaattcttc ctggtcgtgg atgaagatga tccggaatac cagggcgatg gccagatcct
     4921 gtcattttca gttatggatt acaccggatc agcacaggaa tttatttcgg atgatatccc
     4981 tgtcgatatt gagaataatt caatttctat ggcttcagtg atgttctcgc ctgattttga
     5041 gataatcacg atcacaaccg attcgctgcc tccttttgtt gttgacgaac cctatagttt
     5101 ccagcttggt gctgcaggtg gtagaatccc ctattcatgg gaaatcgccg gacaatacac
     5161 ggtcgaacag tcgaccgcca cgatgcccga agtggatgag taccgggtgc tggaatcggc
     5221 ggaagacgac acgatcatgg ccgttgccct cggctttgat tttccgtttt acggtgttat
     5281 gtatgatacc gtgttcatgc acatcaacgg gcatttgcaa tttaccggcg atcagatccc
     5341 ctggccctac atgatggaac cggagctgat gttccgcagt atcccgatga tcgccccttt
     5401 gagctatgca ggacttacca tcatgcccgc ggaaggcgat ggcggatggg tcgaaaccag
     5461 cgacactgcc gccattttcc gctggaagct gtctgccgaa ggtaacctgg gcactaccga
     5521 catcaatttc accgtaaaac tggaggatga tggtactatc gggtttattt acggcccttc
     5581 aacgcttggc gggttcggat ggctggcagg gttatcggcc ggaaacaaaa cagacttcct
     5641 ggtttcgcca tacagtggcc agccgcaaat aacacccgga accagtataa aattcagtta
     5701 tcagtcactc cccggaactt ttcagctttc cgaaaatggg cttttgtcag ggacacccca
     5761 ttccgacgac cgcatccatg atgtggccgt cagggtgacc gaccgatcgg gaatatccgt
     5821 cgcccgcgac ttccagctaa cttctggacc ttacatttat ctttcggtaa tgccgggaaa
     5881 cgatgaccag gtcttctacg gcgatacggt aagcttgcaa ctgaccctgg tcaacggcgg
     5941 cacggagatc ctcgaaaatc ctttcatcag cctttcgacc agcgatccgt tcatcatttt
     6001 cctggattcg cagtgccaga cggggacgat tcctgccggt gaatcggtgt cggtgacaga
     6061 tgctttcagt ttcgtagtat ccacttttat tccagaccat agcgatatcc agttcgaaac
     6121 agtgctcgat gcctccggcc gaaactgggt caaggatctc gtccttcaat ccaacgcacc
     6181 cggactcttt ctcgaaagga atgagatcgg cgacgaagac aaccgtctca gccccggaga
     6241 aacagcgcca ctgctggcca ctttgcgcaa ttcgggccat gccgcaatcg atcaggttat
     6301 gtcggagctg gtctccctca gtcccgaagt ggtggtatcc ggaaatactg tgcaggattt
     6361 cggcagcatc gggaaaggtg cgtctgttac gcggtccttc acgctcactg ccgatgattc
     6421 cactcccagc ggcacaccgg tcacattagt tctgaccaca acatcagcat caggaatcgg
     6481 acaacaggat accatcgaaa tgaaagtcgg gaaaacgccg gtaatggtga tcgatatggc
     6541 cgtcgacaaa aattcgggcc cggtgatctt ttcgcttttg aatgaactca acgttctttc
     6601 tgaatacgat tattcgatca cacccaaaat atttgattac gaatccttgt ttatctgcct
     6661 gggttactat taccagaacc atctgctgac cctgggggaa ggcacgctga tcgccaatta
     6721 cctggatgcg gg
//
LOCUS       DAOQXS010000088         6732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11720_length_6732_cov_3.621237, whole genome shotgun sequence.
ACCESSION   DAOQXS010000088 DAOQXS010000000
VERSION     DAOQXS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11720_length_6732_cov_3.621237"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PKM34_03745"
     CDS             <1..1174
                     /locus_tag="PKM34_03745"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82731.1"
                     /translation="ELNDHGTIINQWCFGGKYYDLGLDIVEVSSGYVIVASTNSNDRD
                     VSGFHGDAGSDYNDIWVFMIDILGNMDWQRCLGGLAVDWPVYITQTSDSGYIIIGNTW
                     SKDGDVTGNHSINDSHSDIWVIKLSENGELEWEHCFGGLGTERFCGTNSVLKLDDYNY
                     VIGANSNYLSDDVECDLFPGNTIDYNAWLFEIKDCDYFKPHVPVIASGPDTVCSTVTA
                     TSVYTIDTATWATGYEWLLIPETAGTISIDSLSAQITWNLTYEGTAELKARGTNDCGY
                     SEWSEAKCVRVHTCLGIEEEETGRPGDKETVRGLEVWPNPASQVLSVKCLGLNSGSDI
                     NLSVYDVFGREVAEIKVPERQDQVQINVESYPPGVYIALLKSGCDFLGSSKFVVAR"
     gene            complement(1214..2722)
                     /locus_tag="PKM34_03750"
     CDS             complement(1214..2722)
                     /locus_tag="PKM34_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82732.1"
                     /translation="MGLNNFTFSRNLSIVTYLLSVAFLLISFQAFARYDYNSNCRQAM
                     QSILDLKFRDARVFIANEKTVHPDNGYAIFLEHYADAIELIVTEEEELYERLIDNFED
                     RMDRMNKLDDGSPDNRWLQAEILFHTGLAQIKYGSRFGGATKLMSSYRKTREHRQKYP
                     DFWQNRKLTGIFNIILDYIPPFMRWAADMFGYSGNAELGLFQLRQYCDDAAATPGFAE
                     EGVMMAALGYKLTWGEPEGFAFLAGQPESMQSKTLVKYLYASAATYTYRNDLALSLLS
                     DIRREDLQVNFYHLDYITGRCKLNHLEPDAAFYLQKYLDEFPGLDYKKDVCNRLSFHF
                     LVHGDMERYREYREMTGRVGQELRDRDQEAVLESRTGCDPHPGLLRARLLCDGGYFND
                     AMQVLLAIRPDEMNIPPFRLEFLYRKGRILQLSGRQDEAIRELTACYDLGAKLPHTFA
                     CRSALYLGKIYEESKDSAKAAEWYEKCIGVYSTSHTTEGIREMASRALKRLK"
     gene            complement(2771..4768)
                     /locus_tag="PKM34_03755"
     CDS             complement(2771..4768)
                     /locus_tag="PKM34_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82733.1"
                     /translation="MGINKQRLPQELMVKKGTSESFRFLLLMLILTVVTLGLNINTVR
                     NYYSLDDFHIAKNNPDFEQGIKSIPKIFVTQYSSRDGKSYGYRPLIRASFAIEYEFFG
                     KNPHISHFINVVFYLAVILLLYLVLRRLLRNYHYLFPFLITLLFAAHPIHTEVVASLK
                     NRDELFMFLFGLLSLQQAIRYADTLKNRHIYWALLFFFLAGLSKPTAAAFFFVIPLSL
                     HFFTDLETKKVIRLTVITGIIALIAAVGPFFYLPAQDRIMAKVENPLAVEGNFLDHIA
                     YAGYSLYYYLKLLIFPHPLRYYYGFNMFPAISLGNIVVILSILVHLGLFAFAIWKLKQ
                     KNIFSYIILVYLTSIVLFTNLFKPVPGIIAERFLLIPSLAFCMLLVLGIFHLFLHHPE
                     KKQISGLKIFGIIGIVALILIPYGSKTMIRNKQWRTEYTLYKADMPYLYDSFKGNDLY
                     ANEIMKSVNRELAKPVNVLKFVDPQVKLAISHWERAIEILPEAASPYRNLGIIYSRVY
                     KEYDTAVVYFNKTLEREPDDPLTYFNLGLAYEGMKDYGKALENLKKSIQIDSAAINAR
                     SRLANIYYGLGEFKEAIRLNQEIMSIDPDESLPYVNIGNYYIFQKDTLNGIKYYERAV
                     ELGAPADASVFLAKYYRLKGNTEKMNYYSRKADEISRKNAQ"
     gene            complement(4882..>5129)
                     /locus_tag="PKM34_03760"
     CDS             complement(4882..>5129)
                     /locus_tag="PKM34_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ82734.1"
                     /translation="IRGMKNRPDRQHVLMNSRVPVLFIIGKNDARIPLSIIIPQTLLP
                     AHSETLLLDHVGHMGFIEASAKTFDALQGFAQRVLSC"
     assembly_gap    5130..5139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5140..5672)
                     /locus_tag="PKM34_03765"
     CDS             complement(<5140..5672)
                     /locus_tag="PKM34_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ82735.1"
                     /translation="MTRIPADKTGMLHFRVTGNGPAIVLLHGFLESSEIWKNFARRLS
                     KSFRVILIDLPGHGASGCIGGEHTMDAMAEAVHFVLINLGIKKCVMAGHSMGGYVTLA
                     FAEKYPRMLKGLALFHSHAAADSPETAANRERTIALVEKDRLGFIKNFVPDLFDPANL
                     KEFSRDIIHLKSITENTT"
     gene            complement(5688..5966)
                     /locus_tag="PKM34_03770"
     CDS             complement(5688..5966)
                     /locus_tag="PKM34_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82736.1"
                     /translation="MANAINDKLRNECQNALEALQKLNIPETTELQAKLAWCIGSYDF
                     DKNPSGLSEFGGMALKELNRFKEKNPRKVAKKMIDGLDSAIRNYETSL"
     gene            complement(6240..>6732)
                     /locus_tag="PKM34_03775"
     CDS             complement(6240..>6732)
                     /locus_tag="PKM34_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ82737.1"
                     /translation="KEMHLLAAKTIATGLKELGLMKGDVDEAVSQGAHALFFPHGLGH
                     MLGLDVHDMEGLGENFVGYDSTIARSSQFGLAFLRFGRKLEPGFVLTNEPGCYFIPAL
                     IDQWRAEGRFTEFINYDKVETYKDFGGIRIEDNVLVTADGNRVLGKPIPKTVDEVEKL
                     CGF"
BASE COUNT         1719 a         1542 c         1748 g         1713 t
ORIGIN      
        1 agaacttaac gatcacggaa caattataaa tcagtggtgc ttcggaggaa aatattatga
       61 cttgggactt gatattgttg aagtttccag tggttatgtt atcgtagcca gcacaaactc
      121 aaatgaccgt gatgtcagcg gatttcacgg tgatgctgga agtgattaca atgacatttg
      181 ggtctttatg atagatatct taggtaatat ggattggcaa agatgcttag gaggattggc
      241 tgtggattgg cctgtttata taacacagac ctcagattcc ggatatatca ttattggtaa
      301 tacctggtca aaggatggcg acgtcaccgg aaatcattcc ataaacgatt cacactcgga
      361 tatatgggtt ataaaactct cagaaaacgg agaactcgaa tgggaacatt gtttcggtgg
      421 tctgggcaca gaacggtttt gcggaacgaa ctctgtcctt aaactggatg actacaacta
      481 tgtgatcgga gcgaattcga actacctgag cgacgatgtc gaatgtgatc tatttccggg
      541 taataccatc gattataacg cctggttgtt cgaaatcaag gactgcgatt atttcaaacc
      601 gcatgtgccg gtcatcgctt ccgggccgga tacggtctgc tccaccgtta cggccacatc
      661 cgtctatacc atcgatacgg caacatgggc aacgggatac gagtggctgc tgataccgga
      721 aacggcagga acaatatcaa ttgattcatt atcagcacaa atcacctgga acctcaccta
      781 cgagggcact gccgagctga aagcccgcgg aacaaacgac tgcgggtatt ctgaatggtc
      841 ggaagcgaag tgtgtgaggg tgcatacgtg tttgggaatt gaggaagagg agacggggag
      901 accgggagac aaggagacgg tgagaggatt ggaggtgtgg ccgaaccctg cttcgcaagt
      961 cttaagtgtt aagtgtttag ggttaaattc gggcagcgat attaatttat ctgtatacga
     1021 tgtgttcgga cgggaagtcg ctgaaatcaa agttcctgag aggcaggatc aggtacagat
     1081 caatgtggaa agttatccgc cgggagtgta tatcgcgctt ctgaaaagtg gatgcgattt
     1141 tttgggaagc agtaagtttg tggtggcgag gtgatgcagg cggagcacga attttaaaaa
     1201 gcaatttcaa aagttatttc aatctcttca gcgccctgga agccatctcc ctgatccctt
     1261 cggtggtatg tgaagttgaa taaactccga tacatttctc ataccattct gcagcctttg
     1321 cagaatcctt gctctcttca tagattttcc ccaggtacag cgccgaacgg caggcaaagg
     1381 tatgcggaag ctttgcaccc aggtcatagc aggcagtaag ttcccggatc gcttcatcct
     1441 ggcggcccga caactgcagg atacgtcctt tccggtagag aaactccagc cggaaaggag
     1501 gtatgttcat ttcatccggc cggatggcca gcagcacctg catagcgtca ttaaaataac
     1561 cgccgtcgca cagcagccgg gccctgagca aaccggggtg cgggtcgcaa ccggtgcggc
     1621 tctccagcac ggcttcctgg tcgcggtcac gcagctcctg ccccaccctg ccggtcattt
     1681 ccctgtattc tctgtacctc tccatatctc cgtgaaccag gaaatgaaac gataaccggt
     1741 tacaaacatc tttcttgtaa tcaagccccg ggaattcatc caggtatttc tgcaggtaaa
     1801 atgctgcgtc gggctccagg tgattgagtt tgcaccggcc ggtgatgtag tcaaggtgat
     1861 aaaaattaac ctgcaggtcc tcccggcgaa tatcggataa aagactcagc gccaggtcat
     1921 tccggtaggt gtaggtcgcg gcgctggcat aaaggtattt caccagcgtt ttcgattgca
     1981 tggattccgg ctggcccgcc aggaaagcaa agccttccgg ctccccccag gtcagcttgt
     2041 aacccagcgc ggccatcatt accccttcct cggcaaaacc gggagtggcc gcggcatcgt
     2101 cgcaatactg cctgagctgg aaaagcccga gctctgcatt gcccgaataa ccgaacatgt
     2161 cggcagccca acgcataaat ggcggaatat aatcgagaat gatattgaaa atacccgtca
     2221 gcttccggtt ctgccaaaaa tcgggatatt tctggcggtg ctccctggtc ttccggtagg
     2281 aactcatcag cttggtagcg ccgccgaaac gggagccgta tttgatctgg gccagcccgg
     2341 tgtgaaaaag gatctcggcc tgcagccacc tgttatccgg ggaaccgtcg tccagtttat
     2401 tcatccggtc catccggtct tcaaaattat ctatcagccg ctcatacagt tcttcttctt
     2461 ccgtgacgat cagttcgatc gcatcggcat aatgttcaag gaaaatagca tagccattat
     2521 caggatgaac cgtcttttca ttggcaataa aaacccttgc atccctgaac ttcagatcca
     2581 ggatagactg catagcctgc cggcaattgg aattataatc atagcgggca aaagcctgaa
     2641 aagatataag caggaatgcg accgaaagaa ggtatgtaac aattgacagg ttccgggaaa
     2701 aagtgaaatt attcagaccc atagtcattg tatatagttc atttcccgaa acttaaaatc
     2761 aaagccagaa ctattgagcg ttcttcctgc ttatttcatc cgctttccgg ctgtaatagt
     2821 tcatcttttc ggtattacct ttcagccggt aatatttggc caggaaaacc gatgcgtcgg
     2881 ccggggcacc cagttcaacg gccctttcgt agtattttat cccgttcagg gtatctttct
     2941 gaaaaatgta ataattgccg atattcacgt aagggagcga ttcatccggg tcgatactca
     3001 tgatctcctg gttcaggcgg atggcttctt tgaactctcc cagcccgtag taaatattgg
     3061 ccaggcggga gcgggcatta atagccgccg aatcgatctg aatgcttttc tttaggttct
     3121 ccagcgcttt accgtaatct ttcatccctt cataggccaa tccgaggttg aaataagtca
     3181 acggatcatc cggctcccgt tcgagggtct tgttgaaata gacgacagcc gtatcatatt
     3241 ctttatagac ccgggaatag atgatcccca ggttccggta gggcgacgct gcttccggca
     3301 ggatctcgat ggctctttcc cagtggctga tagccagttt tacctgcgga tcgacaaatt
     3361 tgagcacatt caccggtttg gcgagctcac ggtttacgct tttcatgatc tcattggcat
     3421 agagatcgtt acctttgaag gagtcgtaca ggtaaggcat atcggcttta tagagcgtat
     3481 attcggtacg ccattgctta ttgcggatca tggtcttgga tccgtaggga atgagtatca
     3541 gcgctacaat accgataatc ccgaagattt taaggccgga gatctgtttt ttttcaggat
     3601 gatgcagaaa aagatgaaat attcccagta caagcagcat gcagaaagcc agtgaaggga
     3661 tcagcaggaa gcgttcggcg atgatgcccg gcacaggttt gaaaaggttg gtgaacagta
     3721 caatggatgt caggtacact aaaataatgt aggaaaagat atttttctgc ttcagtttcc
     3781 agatggcgaa ggcaaacaat ccaagatgga caaggatgga gagaatgacg acaatatttc
     3841 ccaggctgat agccggaaac atgttgaagc cgtagtaata gcgcagcgga tgggggaaaa
     3901 tgagcagttt caggtaataa taaaggctat atcccgcata agcgatgtga tccaggaagt
     3961 ttccttcgac ggcaagcggg ttttcgactt tcgccatgat gcggtcctgc gctggcaggt
     4021 agaaaaaggg cccgacagcg gcgatcaaag cgattatccc ggtgatcacc gtcaggcgga
     4081 tgactttttt cgtctccaga tcggtgaaga aatggagcga caggggtatg acaaagaaga
     4141 acgcggcggc ggtcggtttt gataatccgg ccaggaagaa aaacaggaga gcccaataga
     4201 tatgtctgtt tttcagtgta tcggcatacc ggatagcctg ctggagcgac aggaggccga
     4261 acaagaacat gaaaagttcg tcgcggtttt tcaggctggc gaccacttcc gtatggatcg
     4321 gatgggccgc aaacagaagg gtgatcagga atgggaagag gtagtggtaa tttcgcagca
     4381 aacgcctcag caccaggtat agcagaagaa taacggccag gtaaaagacg acattgataa
     4441 aatggctgat atgggggttt ttaccgaaga attcgtattc gatagcgaaa gatgcccgga
     4501 tcagcgggcg gtaaccgtag cttttgccat ccctggagga gtattgtgtg acaaatatct
     4561 tcgggatcga ttttatcccc tgctcgaaat ccggattgtt ctttgcaatg tgaaaatcgt
     4621 cgaggctgta gtaattcctg accgtattga tattcaggcc gagcgttacg acggtcagga
     4681 tcagcattaa taaaagaaac cggaaggatt cagaagttcc tttcttcacc attaattcct
     4741 gcggtaatct ctgcttattg atacccatac ttaaatgatt ttactgcaaa gtaaagcaaa
     4801 attcgtttga cccgtgttat ccgtgttcct cttataagaa cacaaataga cacggatgtg
     4861 acgaatattc acgaataaga attagcagga aagaactctt tgtgcgaatc cttgcaaggc
     4921 atcgaaggtt tttgcagagg cttcgataaa tcccatatgg ccgacatgat ccaggagcag
     4981 cgtttcgctg tgagccggca gcagcgtttg cggaatgatt attgacaacg gtatccgggc
     5041 gtcgttttta ccgataatga ataacaccgg tacccggctg ttcatgagaa catgctggcg
     5101 gtcgggacgg tttttcatcc cgcggatggn nnnnnnnnng tcgtgttctc ggtaattgat
     5161 tttaaatgta tgatgtccct ggaaaattcc ttcagattgg cgggatcgaa gagatcggga
     5221 acgaaatttt tgataaatcc caggcggtct ttttccacca atgcgatggt cctttcgcgg
     5281 tttgcggcgg tttccgggct atcggcggcg gcatgggagt gaaagagggc caaacccttc
     5341 agcattctcg gatatttctc cgcaaacgca agggtgacgt aaccgcccat ggagtggccc
     5401 gccatcacgc atttttttat tccgaggttt atcaagacaa aatgcacggc ctctgccata
     5461 gcatccatgg tatgttcacc accgatgcaa ccggaagcgc cgtggccggg caaatcgatc
     5521 aggatgaccc tgaacgattt gcttagcctt cgggcaaaat ttttccagat ctccgatgat
     5581 tccaggaatc cgtgaagaag gacaatagcg gggccgtttc cggtgaccct gaaatgaagc
     5641 atgccggttt tatccgcggg aatccgtgtc atccgcgtac cgtatgctta caggctggtt
     5701 tcgtaattgc ggatggcgct gtcgagcccg tcgatcatct ttttagcaac ctttctgggg
     5761 ttcttttcct tgaagcggtt aagctctttc agtgccattc cgccgaattc gctcaggccg
     5821 gaagggtttt tatcaaagtc atagctaccg atgcaccatg ccagtttggc ctgtaattca
     5881 gtagtttcgg ggatattcag tttctgaagg gcttccagtg cattctggca ctcgtttctt
     5941 aacttatcgt tgattgcatt tgccatttta atgaggatta aaggattatt aaaaaagagt
     6001 gcaaaagtaa ccattttatt tagaatagtt ctttgatcga gagagaaaat atattgtgag
     6061 ggtgatggta agaagaggtt gccttaaaag tgtttaacca caaaggcgca cgaaggatta
     6121 cactaaggag cacaaaggtt tcccactgtt aatcaattac ttggtgtgcc tttgtgaagg
     6181 cccttcgtgc gcctttgtgg tttaacaaaa gacttttgat acaacctgtt ctgaccccac
     6241 taaaacccgc aaagcttttc cacctcatca accgtcttcg gaatcggttt gcccagcacc
     6301 cggttcccgt cggcagttac cagcacattg tcttcgatcc tgatcccgcc aaagtctttg
     6361 taagtttcga ctttgtcgta gttgatgaat tcggtaaacc tgccttcggc tctccactgg
     6421 tcgatcaggg cgggtatgaa gtagcatccg ggctcgttgg tcaggacaaa tcccggctcg
     6481 agcttccggc cgaaccggag gaaagccagg ccgaactggc tgctgcgggc gatggtgctg
     6541 tcgtagccga cgaaattctc gccaaggcct tccatatcgt ggacatcgag gccgagcata
     6601 tggccgaggc cgtgggggaa gaaaagggcg tgggcaccct gtgacacggc ttcatccacg
     6661 tcgcctttca tcagcccgag ctctttcagg ccggtggcga ttgttttggc tgccaggagg
     6721 tgcatctctt tg
//
LOCUS       DAOQXS010000089         6712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11773_length_6712_cov_3.238696, whole genome shotgun sequence.
ACCESSION   DAOQXS010000089 DAOQXS010000000
VERSION     DAOQXS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11773_length_6712_cov_3.238696"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1585
                     /locus_tag="PKM34_03780"
     CDS             155..1585
                     /locus_tag="PKM34_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ82738.1"
                     /translation="MEYRFKSYLDLPVFKPLLAAVKQYGHPAYVVGGYVRDRILSRTT
                     KDIDVVVIGSGIELAEIYAQKIGKGASVNVFRNFGTAMVRFHEQDVEWVVEFVGARKE
                     SYRANSRKPIVEDGSLEDDQNRRDFTINTLAISLNSKDLGTLLDPFGGFHDMEECLIK
                     TPLSPDKTYSDDPLRMMRAIRFATQLDFVIEKGSFESIRRNADRINIISRERINDELE
                     KIILSKKPSVGFNLLMDSGLLKLIFPEFVALKGAEYIDGIGHKDNFHHTLQVLDNVAA
                     KSDNLWLRWGAILHDIAKPVTKKFEQGAGWTFHAHEFVGAKMIPKLFQRMRMPVDERM
                     KYVQKMVLLHLRPIVLSQEEVTDSAVRRLLFDAGDDIDDLMLLCEADITSKNREKVIR
                     YMKNFELVRKKLVEVEAKDRIRNWQPPVTGELIMETFGIPPGKEVGIIKNAIREAILD
                     GIIHNDFDEAYRLMIAEGERLGLRKC"
     gene            1603..2514
                     /gene="miaA"
                     /locus_tag="PKM34_03785"
     CDS             1603..2514
                     /gene="miaA"
                     /locus_tag="PKM34_03785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNQ82739.1"
                     /translation="MSSPVAKTLLIILGPTASGKTELAIRAAQWLDTEIISADSRQFY
                     RELSIGTAKPSPEQLSAVRHHFIGHLSVTEDYNIARFENDAIELLEVLFEKHDVVVMC
                     GGSGLYIDAVCHGIDEQPEHNPQIRKQLTEKYREKGIGYLQEQLQKLDPGYFNTVDIS
                     NPQRLLRAVEVCLITGKPYSGYRKGELKDRSFRIVKIGLEISREKLTERINRRTDEMI
                     GAGLVDEVKGLFSYRHLNALNTVGYKEIFCYLDGEWSLEEATEKIKINTRRYAKRQMT
                     WFRKDKTVTWIDPELFSPVVLHEALQR"
     gene            complement(2473..3315)
                     /locus_tag="PKM34_03790"
     CDS             complement(2473..3315)
                     /locus_tag="PKM34_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ82740.1"
                     /translation="MILIADAGATKIQWVVIHDGKAGNPFETNGFNPYFMAPKILEDA
                     VEKDLVPFLSPPHIKQIYYYGAGCSTVQKCGIVDDVLRDIFENADIDIHHDLLGAARS
                     LFGKVEGIACILGTGSNSCLYDGKTILENVTSLGYLFGDEGSGAYMGKLFLTEYLRKR
                     MPQHLTEEFDRKFGFTVENILDSVYNKPHPNQFLSSFSKFIGEHKEDEFIKTIARQNF
                     RDFFREQVTRYTDYKQKPLGVIGSVGFHYQEIFREVASEHGLAVSKLVQSPLQGLVEY
                     HGGK"
     gene            complement(3552..4937)
                     /locus_tag="PKM34_03795"
     CDS             complement(3552..4937)
                     /locus_tag="PKM34_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNQ82741.1"
                     /translation="MRYIGFNYQAFVAFLIILTGIHFISVAQDDNDQKNSPVQVYKFD
                     IKKEIAPPVWHSTKKALEEAVAQNADVILIEMSTYGGMLESADSIRTKILNSPIPVIC
                     FIHHNAASAGALIAIACDSIYMSTGSSIGAATVVDQAGQVVPDKYQSYMRAMMRATAE
                     ATGRDPNIAQAMVDPRIEIEGVIDSGSVLTFTASEAMKFGYCNGIAETVDEVIGKAGI
                     KDYEIIEQKLTAIDKIIGFLISPVISGILIMVIVGGIYFELQTPGIGFPSIAAVTAAL
                     LYFAPLYLEGLAAHWEILIFIVGIILVAIEIFAIPGFGIAGISGIVLIITGLTLSMIG
                     NIGLDFRPVNFNNFIVSFFIVIISIFLSIIGSYFLTKSMFSRNRIFGSLALETVESAE
                     EGYTTSDSSYREMVGKTGTAHSILRPVGKVNIGGEIYDATALTGYIDKGEKIEVIRYE
                     TTQLFVRKIEN"
     gene            complement(4954..5757)
                     /locus_tag="PKM34_03800"
     CDS             complement(4954..5757)
                     /locus_tag="PKM34_03800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNQ82742.1"
                     /translation="MNFERLVREVVELCRNDGQYIRQQLELLRAKDIELKDDHNFVTY
                     VDRNSEAMLVENLSKLTPGCGFIAEENDYGSSGAGLKWVIDPLDGTTNFVHGIPLFAI
                     SVGLMEGDRVVAGVVHEVNRGESFYSWGDGRAYLNGKEIRVTEALTLKNSLLVTGFPY
                     IRDHILDEYMNLFKEYIRRSQDLRRLGSAATDLAYVAAGRFEGFYEISLNSWDVAAGS
                     FLVEQAGGRVTDFQGGDDFIFGRSLIASNGFVHDEMLAVAGEFFNIKAK"
     gene            complement(5814..>6712)
                     /locus_tag="PKM34_03805"
     CDS             complement(5814..>6712)
                     /locus_tag="PKM34_03805"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ82743.1"
                     /translation="AGLMNAVKVVGKEKEKIIFAQPRKKKRGKPPVLETVMVRPRELT
                     TNIILVAAVSGVIGAKIFDVIEHLDRLFEDPAGTLFSFSGLAFYGGLITAAFTVSWYV
                     ERHKIPWAVNADAVAPGLLLAYGVGRIGCQLSGDGCWGIVNPEPKPEWLSFLPDWMWA
                     FNYPHNVINEGVLIPGCSRANCHVLPEAVFPTPFYETIMALILFGILWSIRKKFSIPG
                     YMFSTYLILNGIERLIIEQFRVNRRYDLLGMEVTQATVIAVGLILAGIAGFFLFRRFH
                     NRKVSILNTKNTRDSTKGHKRS"
BASE COUNT         1665 a         1682 c         1740 g         1625 t
ORIGIN      
        1 tgggaccaaa cccacccgga tcaagctgaa agcaaaaccg accattctca tcggttccac
       61 cccgctggaa taccccggat acatcaccat ccggcaggaa tttggggcac agtaagccgg
      121 gatttgcccg ttttgcttac ttttgcatcc gctgatggaa taccgcttca aatcatatct
      181 tgatcttccc gtattcaagc cgttgctggc ggccgtgaag caatacggtc accccgctta
      241 tgttgtgggg gggtatgtcc gcgaccggat cctgagccgc acgaccaaag atatcgatgt
      301 tgtggtgatc ggcagtggca tcgagctggc ggagatctat gcgcagaaaa tcggaaaggg
      361 agcatcagta aacgttttcc gcaatttcgg caccgccatg gtcaggtttc acgagcagga
      421 tgtagagtgg gtcgttgagt ttgtcggggc caggaaagaa tcctaccggg ccaattcacg
      481 caaaccaatc gtggaagacg ggagcctgga agacgaccag aaccggcgcg attttacgat
      541 caatacgctg gctatcagcc tgaacagcaa agacctggga acgctcctcg atcctttcgg
      601 cggatttcac gatatggaag aatgccttat caaaacaccc ctttcacccg ataaaaccta
      661 ttccgacgac ccgctgagga tgatgcgggc catccgtttt gccacccagc ttgattttgt
      721 cattgaaaaa ggctcatttg aatcgatccg caggaatgcg gatagaataa acatcatttc
      781 ccgggaaagg atcaacgatg aactggagaa gataattctc tcgaaaaagc cctccgtcgg
      841 gtttaacctg ctgatggact ccggcctgtt aaagctgatc tttccggagt ttgtggcgtt
      901 gaaaggggct gaatacatcg acgggattgg gcataaggat aattttcacc atacgttgca
      961 agtacttgac aatgttgcgg ctaaatctga caatctctgg ctgcgctggg gggccatttt
     1021 acacgatatt gccaaaccgg tgacaaagaa gtttgaacag ggcgccggct ggacatttca
     1081 cgcccacgag tttgtcggcg cgaagatgat cccgaagctg tttcagagaa tgcgcatgcc
     1141 ggtggatgaa cggatgaaat atgtccagaa gatggtgctg ctgcacctcc gcccgatcgt
     1201 tctttcacag gaagaggtga ctgattcggc cgtcaggcgc ctgctgttcg acgccggaga
     1261 cgatatcgac gacctgatgc tgctttgcga agccgatatt acctccaaga accgtgaaaa
     1321 ggtgatccgg tatatgaaaa acttcgagct ggtgaggaaa aaactggtgg aggtagaggc
     1381 gaaagaccgg atcaggaact ggcagccacc ggtcacgggc gagctgatca tggagacatt
     1441 cggcatcccg ccgggaaaag aggtggggat cattaaaaat gccatccgcg aagcgatcct
     1501 cgacggaata atccataacg acttcgatga agcgtaccgg ctgatgattg cagaaggaga
     1561 gagactggga ctaaggaaat gttgaatgtt taatgttgag ttttgagttc acctgtcgcg
     1621 aaaacattgc ttataatttt agggccgacg gcatcgggaa agaccgaatt ggcgatccgg
     1681 gcagcgcaat ggcttgatac ggagatcatt tcggccgatt cgcggcagtt ttacagggag
     1741 ctctccatcg gaacggcaaa accatcgccg gagcaattat ctgccgtaag gcatcatttt
     1801 atcggccatc tttccgtaac cgaagattat aacattgcca ggtttgagaa cgatgcaatt
     1861 gaattgcttg aggtgttgtt tgaaaagcac gacgttgtcg tgatgtgcgg tggatcagga
     1921 ctttatatcg atgccgtttg ccatgggatc gatgaacaac ccgagcacaa tccgcagatc
     1981 cggaaacaat tgacggaaaa atacagggag aaaggcatag gatacctgca ggagcaatta
     2041 cagaaactcg atcccggtta tttcaatacc gtcgatattt ccaatccgca gcgcctgctg
     2101 cgggctgtag aggtttgcct gatcaccggt aagccctatt ccggttaccg taaaggcgag
     2161 ctgaaagacc gttcgttcag gattgtcaaa atagggttgg agatttcaag ggagaagctg
     2221 acagagcgga tcaacaggcg aaccgatgag atgatcgggg cgggactggt cgacgaagtg
     2281 aaaggactgt tttcataccg gcacctgaat gcactgaata ccgtcggcta caaggaaata
     2341 ttctgttacc tcgatggaga atggagcctg gaagaggcga ctgaaaagat aaagatcaat
     2401 acgcggcggt atgccaaacg gcagatgacc tggttcagga aggataaaac cgttacctgg
     2461 attgacccgg aattattttc ccccgtggta ctccacgagg ccctgcagcg gtgactgcac
     2521 cagttttgaa actgccaggc cgtgctccga agctacttcc ctgaatatct cctggtaatg
     2581 aaaacccacg gaaccgatca cacccagcgg cttttgtttg taatcagtgt accgggtcac
     2641 ctgttccctg aaaaaatcac gaaaattctg ccgggcaatc gttttgataa attcatcctc
     2701 tttatgttcc ccgataaatt ttgagaaaga ggagaggaac tggttgggat gaggtttgtt
     2761 ataaacggaa tccaggatat tttccaccgt gaagccgaat ttgcggtcaa actcttctgt
     2821 aagatgctgc ggcattcttt tcctgaggta ttcggtcagg aacagcttgc ccatgtaggc
     2881 gccgctgcct tcgtcgccga aaaggtagcc cagcgaagtg acattctcca ggatggtttt
     2941 cccgtcgtac aggcacgaat tggatcccgt tccaaggatg caggcaatgc cttccacttt
     3001 tccgaacaat gaccgggcgg ccccgagcag gtcgtgatgg atatcgatat cggcattctc
     3061 aaagatatca cgcagcacat cgtcgacgat accgcatttc tgaacggttg aacatccggc
     3121 gccgtagtaa tagatctgtt tgatatgcgg gggactcaga aagggtacca ggtctttttc
     3181 cacggcgtct tcgaggatct tcggcgccat gaagtaggga ttgaaaccat tcgtttcaaa
     3241 aggattgccg gcttttccgt cgtggatgac cacccattgg atcttggtcg ccccggcatc
     3301 tgcaattaat atcattcaaa ataattttgc caaagataaa ggaataatcg gggataaagg
     3361 atacgggagc agaatctgga atttacaatt tacaatttac agtttacagt ttgcgatctt
     3421 agattttttc agatcgacca tcgaaaatcg aatatgaaag ttgggcgttc cgggtcccgg
     3481 agttccttag gccccggaaa ggtgcaagta gtgaggcatt ttatcccgga aacgcccaat
     3541 ggtttcagct atcagttttc aatctttcta acgaaaagtt gtgtggtttc gtacctgatg
     3601 acctcgattt tctccccttt gtcgatatag ccggtcaggg cggtagcgtc gtagatttcg
     3661 ccgccgatat tcacttttcc caccggtcgc aggatcgaat gggccgtgcc cgtttttccg
     3721 accatctccc tataactgct gtcggaagtc gtgtatcctt cctcggcgct ttccacggtt
     3781 tccagcgcca gcgacccgaa gatccggttt cgtgagaaca tcgatttggt caggaaatag
     3841 gaaccgatga tcgaaaggaa gatcgagatg atcacgatga agaaggagac gataaagttg
     3901 ttgaagttga caggcctgaa gtcgaggccg atattcccga tcatgctgag ggtcaggccg
     3961 gtgattatga gtacgatacc ggaaatcccg gcaataccga agccgggtat ggcaaagatc
     4021 tcgatggcca ccaggatgat cccgacgata aagatgagga tttcccagtg tgcggccagc
     4081 ccttcgaggt agaggggcgc gaaatagagg agtgcagcgg tgacggctgc gatggaaggg
     4141 aaaccgatcc cgggcgtctg gagttcgaaa tagattccac cgacgatgac catgatcagg
     4201 attccgctga tgaccgggct gatcaggaac ccgatgattt tatcgatggc ggtgagcttc
     4261 tgctcgataa tttcgtagtc ttttatgccg gctttcccta tgacttcatc gacagtttcg
     4321 gctattccgt tgcagtagcc gaatttcatt gcttccgatg cggtgaaagt gaggacgctg
     4381 cccgaatcga tcaccccttc gatttctatt ctcgggtcga ccatcgcctg ggcaatattc
     4441 gggtctctgc cggtggcttc ggcggtggca cgcatcatgg ccctcatgta cgactggtat
     4501 ttgtcgggca ccacctggcc ggcctggtcg accaccgtag ctgccccgat ggaagagccg
     4561 gtcgacatgt agatgctgtc gcaggcgata gcgatcaaag ccccggccga tgcggcgttg
     4621 tgatggataa agcagatcac cgggataggg gagttgagga ttttggtacg gatggagtcg
     4681 gccgactcca gcataccgcc gtaggtgctc atttcgatca ggataacatc ggcattttgc
     4741 gccaccgctt cttccagcgc ttttttcgtc gaatgccaga ccggcggtgc gatttctttt
     4801 ttgatgtcga atttatacac ctgtacaggg ctgttcttct gatcattatc atcctgggca
     4861 acagaaataa agtgaattcc ggtcaggata attaaaaatg caacgaatgc ctggtaattg
     4921 aatcctatat atctcatcta ctattattta gtgttatttt gcttttatgt taaaaaattc
     4981 ccctgccacc gccagcattt cgtcatgcac gaaaccgtta ctggcaatca gtgaccggcc
     5041 gaagataaag tcatcacccc cctggaaatc ggttactctt ccaccggctt gttccaccag
     5101 gaatgatccg gcggccacat cccaggaatt caggctgatc tcatagaacc cttcgaatcg
     5161 tcccgctgcc acataagcca ggtcggttgc cgccgagccc agccggcgca aatcctggct
     5221 ccggcggata tactccttga acaggttcat gtactcatcc aggatatgat cccggatata
     5281 tggaaatccg gtgaccagaa gactgttctt caaggtcagg gcttctgtta ccctgatctc
     5341 ctttccgttc agataggccc gcccgtcccc ccaggaataa aaactttctc cccggttgac
     5401 ttcgtgcacc acgcctgcaa ccacccggtc tccttccatc aggccgacac tgatggcaaa
     5461 taacggaatc ccatgcacaa aattggtggt gccgtcgagc ggatcgatta cccatttcaa
     5521 cccagcgccg gaactcccgt aatcattctc ttcggcaata aaaccgcagc ccggcgtcaa
     5581 tttcgataga ttctccacca gcatggcttc cgaattccgg tcaacatagg tcacaaaatt
     5641 gtgatcgtct ttcagctcga tatctttagc ccggagcagt tcaagctgct gccggatata
     5701 ttgtccgtcg ttccggcaca gttcaacaac ctctcggacc agtctttcaa aattcatggt
     5761 tgatatattt tctgaacagt caagctgtgt aattcccgaa ggcgcaagaa gtttcaactt
     5821 cttttgtgcc cctttgtgct atcccttgtg ttctttgtgt ttaaaatact tacttttcta
     5881 ttatgaaatc gtctgaataa aaagaatccg gctatgcccg ccaggattaa acccacggca
     5941 atgaccgttg cttgtgtcac ctccattccc agcagatcat aacgcctgtt gacccggaac
     6001 tgctcgatga taagccgctc tattccattc aggatcaaat aagtggagaa catgtacccc
     6061 ggtatggaaa actttttccg gatgctccag aggatgccga aaaggatcag tgccatgatg
     6121 gtttcataga atggcgtcgg aaaaacggcc tcgggcaaaa catggcagtt tgcccgcgag
     6181 cagccgggga tcagcacccc ttcgttgatc acattgtgcg ggtagttgaa tgcccacatc
     6241 cagtcgggca ggaaggagag ccactccggc ttgggttcgg gatttacgat tccccagcac
     6301 ccgtcgcccg aaagctggca tccgatccgt ccgacaccgt aagccagcaa cagcccgggt
     6361 gcaacagcgt cggcatttac agcccatggg atcttgtgcc tttcgacata ccaggagacc
     6421 gtgaatgccg cggtgatcag gccgccgtaa aaggcgagcc cgctgaagga aaacaaggtg
     6481 ccggccggat cttcaaataa acggtcgaga tgctcgatca catcgaatat tttggcgccg
     6541 ataactcccg agacagccgc taccaggatt atatttgtcg tcagttcccg gggccttacc
     6601 atcactgttt ccagtaccgg cggttttcct ctttttttct ttcggggctg tgcgaaaatg
     6661 atcttctctt tctctttgcc aacaaccttg acggcattca tcaatccggc ca
//
LOCUS       DAOQXS010000090         6710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_11776_length_6710_cov_3.841172, whole genome shotgun sequence.
ACCESSION   DAOQXS010000090 DAOQXS010000000
VERSION     DAOQXS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11776_length_6710_cov_3.841172"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKM34_03810"
     CDS             <1..365
                     /locus_tag="PKM34_03810"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNQ82744.1"
                     /translation="KAYKTQISKGYDTDRGRVYLKYGPPNIISENYNEPSSYPYEIWH
                     YYELGGNQRNKKFVFYTQNIITNNFQLLHSDAIGEIQNYKWQVFLNNRWYDPYNVDAN
                     QAPGIYGGKADDYYRNPR"
     gene            427..1308
                     /locus_tag="PKM34_03815"
     CDS             427..1308
                     /locus_tag="PKM34_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ82745.1"
                     /translation="MAFLYHTFKLLANLVSRLPFPVLFFVAGIFNFFLHYIIRYRRDI
                     ILKNLRNAFPEKKPGEIRKILGQYYKNLSQIMFEVIKLHSIRPEKLLERFTFTGLEPL
                     AETLRKGQSVIITIGHCGNWEWMGTALGQVLPARGFAIVKPLSDKHFNRYMESLRHRI
                     NPDSTIPFHRTYRTLIRHKQEMVTFNVFAADQTPVKEEANYWTRFLNQDTPFYIGVEK
                     LAKALHLAVFFIDIQRTGNGKYNGEIRQITREPEKTAEKEITEAYIRLLEEAIRRRPD
                     NWLWSHRRWKLTRTPGN"
     gene            1365..2393
                     /locus_tag="PKM34_03820"
     CDS             1365..2393
                     /locus_tag="PKM34_03820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ82746.1"
                     /translation="MKTAVVILNWNGREFLEKFLPGVIAHSAGEAEIIVADNDSADDS
                     LAFLEAAHPGIRIIRLDKNYGFAGGYNRALKQVDAEYFILLNSDIEVTENWITPVIRL
                     MESDASIAACQPKIRSYKEPEQFEYAGAAGGFIDKYGYPFCQGRLFQHIENDEGQYDQ
                     AREVFWATGACLFVRSVVFTQLGGFDDDFFAHMEEIDLCWRAKNQGYKIMYCPESTIF
                     HIGGGTLPKKNSRKTYLNIRNNIIMLYKNLEPGRLLSVMLARFILDGVAAMKFLVDGG
                     FKDMFAVFKAHIYFWSHLYLLRKKREKIPHHRVSEIYWGNIVVEHYLRRKKSFGQLDP
                     ERFTKNPR"
     gene            complement(2374..>2769)
                     /locus_tag="PKM34_03825"
     CDS             complement(2374..>2769)
                     /locus_tag="PKM34_03825"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNQ82747.1"
                     /translation="LNLLGKREPEIYGNQSFDEFLDILRAEFPGHSIGYYQSNTEGEI
                     INAIQDAGFTTDGIIINPGGYSHTSVAIADAMKAVPARVIEVHISNVFQREDFRKVMV
                     TATGADALICGFGLESYRIAIRNLTGDSL"
     assembly_gap    2770..2869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2870..2954)
                     /locus_tag="PKM34_03830"
     CDS             complement(<2870..2954)
                     /locus_tag="PKM34_03830"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNQ82748.1"
                     /translation="MNLFIINGPNLNLLGKREPEIYGNQSFD"
     gene            3417..3845
                     /locus_tag="PKM34_03835"
     CDS             3417..3845
                     /locus_tag="PKM34_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82749.1"
                     /translation="MKRLIHIIAVISVIFFIASQSLNAQITIKVTLDGQCPDTCTSHE
                     ICTYKVEYQLYNICVEPPELCCTAPPQWVNCSTEEIYFDCDFSCDDPSNEKCYALVGI
                     ATKYCNGPGGNVAKCTGNGVHYYTCNELMTLPNVPVTVLW"
     gene            4914..>6710
                     /locus_tag="PKM34_03840"
     CDS             4914..>6710
                     /locus_tag="PKM34_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82750.1"
                     /translation="MIENPWIRGDGCSLVTNVLNLFPNQVGWGFPNILVDYLLRFEWT
                     GETCQGYPIHFKPNFIPTPQTIQWNFDDGPGSNSWQLSPTYTFKNPGVHEVKVDVWYP
                     SGRYEHTSREIEILPSPQPDLGPDTLICKDATLVLSANCEADFFSWSTGQFGVSSITV
                     SDSGTYWVRGRFNNSGCMGYDTIHVGFHAPTIIDETNLIITQTTCNGASGSITGLIAL
                     GYSPFAYRWKDLSEVQVGTDIDVSGLPAGQYYLTITDANGCETVSNVYTIEDAGNLQV
                     LNVEQTRPHCGRPDGQIIIHALSPAGSALQYSIDDGDSYQTDSVFSGLIGAGYVVRVT
                     DGVGCFGFYNDNPVLLDDIPGPQVVDVIVTDETDFLGNGAIEIVATGSTPVIFYSIDS
                     GATYQLNNGTFNNIGSGIYNLQIWDENGCDTTFTVEIQNIILTWLHAVTGEGGHCLGN
                     TAMVPVNVDNFNAVADFHLKLGYNAGNLECEGFANVNVQLLDSLTGWVDAIAGDIHLT
                     WNGELPLTFIQPEKVADLVFTTKNPGQGELSWYTGETESYFTNSSGNPIPAEFQTGEV
                     KIYEPPEIILDQSKTVSTGQFVSLMSIAVGNQN"
BASE COUNT         1814 a         1519 c         1498 g         1779 t
ORIGIN      
        1 ataaggccta caaaacacag atcagcaaag gatacgatac cgaccgcggc agggtttacc
       61 tgaaatacgg cccgccgaat attatcagcg aaaactataa cgagccgagc agctaccctt
      121 atgaaatctg gcattattat gaacttggcg gcaaccagcg gaataaaaag tttgtcttct
      181 atacccagaa catcattacc aacaacttcc agcttttaca ctccgatgcc atcggcgaga
      241 tccagaatta caagtggcag gttttcctga acaaccgctg gtatgacccc tataacgtgg
      301 atgccaacca ggcccccgga atttatggcg gaaaagcgga tgattattac cgtaacccgc
      361 gttaaacggt cattacaatt ttttgaacgt ttgatagtat ctttgccatc ctgaatctta
      421 gggcaaatgg cattcctgta ccatactttc aaactcctgg ccaacctggt ctcacggctc
      481 ccattcccgg tactattctt cgtcgcgggt atctttaatt ttttcctgca ttatatcatc
      541 cgctaccgga gagatatcat ccttaaaaac ctccgtaacg cgtttcctga aaaaaaaccc
      601 ggagaaatac gaaaaatcct tggccagtat tataaaaacc tgtcgcagat catgtttgaa
      661 gtgatcaagc tgcattctat ccggcctgaa aaattgctgg aacggtttac ttttacggga
      721 ctggagccac tggctgagac gctgcgtaag ggccagagcg tcatcataac tatcgggcac
      781 tgcggtaact gggaatggat ggggacggcg ctggggcagg tgctgcccgc cagggggttc
      841 gcaattgtca aacccctctc cgacaaacat tttaaccggt atatggaatc tctccggcac
      901 cggatcaacc ccgacagcac gatccccttt caccgtacct accgcaccct gatccggcat
      961 aaacaggaaa tggtgacgtt taatgtgttc gccgccgatc agacgccggt gaaagaagag
     1021 gccaactact ggactcgatt tttaaaccag gatacccctt tttacatcgg ggtggagaaa
     1081 ctggcaaaag cccttcacct tgccgtcttt ttcatcgaca tacaacggac cggcaatgga
     1141 aaatacaacg gagagatcag gcagatcacc cgggagccgg aaaaaacggc agagaaggaa
     1201 attacggaag cttatatccg gttgctcgaa gaagctatcc gccggcgtcc cgacaactgg
     1261 ctgtggtcgc accgccgctg gaagctgacc cgcacacccg gtaattgacc cggtattact
     1321 atttctgata attttgattt acatttgaag cagaaatcac ctgtttgaaa acggctgtcg
     1381 tcatacttaa ctggaatgga agggaatttc tggagaaatt ccttcccgga gtcattgccc
     1441 acagcgccgg tgaagccgaa atcattgtcg ccgacaacga ttctgccgac gattctctgg
     1501 ccttccttga ggctgcccat cccggcatac gcatcatccg gctggataaa aattacggtt
     1561 tcgccggagg gtacaaccgg gcgctaaaac aggttgacgc tgagtatttt attctgctga
     1621 acagcgatat tgaagtgacg gaaaactgga tcacaccggt catccggctg atggagtccg
     1681 atgcgtccat tgccgcctgc cagcctaaga tcaggtcgta caaagagccg gagcagttcg
     1741 aatacgccgg cgccgcggga ggtttcatcg ataaatacgg gtacccgttc tgccaggggc
     1801 gcttgttcca gcatattgaa aatgatgaag ggcagtatga ccaggcgcgg gaggtgttct
     1861 gggccacagg ggcctgcctg ttcgtcaggt cggttgtatt cacgcaactg ggtggctttg
     1921 atgacgattt ttttgcccac atggaggaga tcgatctttg ctggagagcg aaaaaccagg
     1981 ggtataaaat catgtattgc ccggaatcaa ctatatttca catcggagga ggaacgcttc
     2041 cgaagaaaaa ctcgcgcaag acctatctca acattcgcaa caacatcatt atgctttaca
     2101 aaaacctgga gccgggcaga ctgttaagtg taatgctggc ccgttttatc ctcgatggag
     2161 tagctgcaat gaaattcctg gtcgacggcg gttttaaaga tatgttcgcc gtgttcaaag
     2221 cccatattta cttctggagc catctatatt tgctcagaaa gaaaagggaa aagatcccgc
     2281 accatcgcgt ttcagaaatt tactggggaa atatcgtggt cgagcattac ctgagaagga
     2341 agaaatcatt cggacagcta gatcctgagc ggtttacaaa gaatccccgg taagattccg
     2401 gatagctatc cggtaacttt ccaggccaaa accgcaaata agcgcatccg cgccggtagc
     2461 ggttaccatg accttacgaa aatcttcccg ctgaaaaaca ttcgagatgt gaacctcgat
     2521 caccctggca ggaaccgctt tcattgcatc ggcaatcgct accgaggtat ggctgtaacc
     2581 tcccgggttg atgataatgc catccgtagt aaatcccgca tcctgtatcg cgttaatgat
     2641 ctccccctcc gtattcgact ggtaatagcc gatggaatga cccggaaatt cggcccttaa
     2701 gatatccaga aactcgtcaa atgactggtt gccatagatt tccggctcgc gcttgccaag
     2761 caggttgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtcaaatgac
     2881 tggttgccat agatttccgg ctcgcgcttg ccaagcaggt tgagattagg accgttgata
     2941 ataaacaagt tcaaagttca aagttcaaag ttcaaagtta gacatttatt cggaatgtca
     3001 gctttatcag ggatctgcca gccggctgtc caactgcccg actgcccaac tgtccgactg
     3061 cccaactgtc caactgtcca actgcccgac tgcccaactg tccaactgcc caactgtcca
     3121 actgtccaac tgaaatccct tgcatttctt cctaaaacat cttactttta tgccaatcat
     3181 ttcaaaatga tcttaatcat gaaattccaa atttcattcg ccaactggcg gaccaaattc
     3241 caatcaattc caaaatccaa tactcaaact ctaaaattaa atcgacaatc gacaatcgaa
     3301 tatcattttc tctcattccc atcctccata aggggtttgt ccccctgaat gaaaattatt
     3361 tgcccgtctt ttaaacagca tccattagaa tttattaact ttaaaaacca tttaaaatga
     3421 aaagattaat ccatattatt gctgtcattt cggttatttt ttttatcgca tcgcaaagcc
     3481 taaatgccca aattaccatt aaagtaacat tggatggtca atgccccgat acgtgtactt
     3541 ctcatgaaat atgcacatat aaagttgaat atcaactgta taatatatgt gtcgaaccac
     3601 ctgaactttg ctgcactgct cctccgcaat gggtgaattg ttcaacggag gaaatatact
     3661 ttgattgtga tttctcatgt gatgacccat caaatgagaa atgttatgcc ttggttggca
     3721 tagcaacaaa atactgcaat ggacctggcg gaaatgtagc aaaatgcaca ggtaatggag
     3781 ttcattatta tacttgcaac gaattgatga ctcttccaaa tgtgccggtt actgttttat
     3841 ggtgataaat tataatgaaa gtctgcccgg cgttacgtaa cgccggcaga ttttttaaat
     3901 tttcaaaatg catgagaaag ctatttactg tactattttt atttgtgatt tataaattat
     3961 ctatcgccca gaatgaagct aatatattat acttcacaca tggttgcggc ttggatttca
     4021 atatgggaat tcctgaagtt ctgtatgatg gacaggcagt acatggttca gcaagttcaa
     4081 ctatcagtga tacatccggg agtttcttgt tttgtgttga tacaaaatat atatattcaa
     4141 tttatggcat catgcaaaat agtgacggtc tggttaatac aggaatatca ggtttggcca
     4201 cggtcaaatg gcctggacag gattctctat attatgtatt tacagctgcc gatcctaatt
     4261 taccctatgc cggattctat tattccatcg tagatattag cctggggctt ggcggtgtgg
     4321 taacagaact aaatgttcca gttccagaat gttgggatgt gagtgatcgc attgcaatca
     4381 caaagaagga taaatcggat gatgtttggg taatagtcag aaagaatgaa gaggaggcca
     4441 ttgcagcatt ccttatcgat gaaaatggat ttcattccga cccggttatt tcagcaatgc
     4501 cacatatcga aatgacctgg caaagtatgt gggggtatat aaaaatctcc tctgataaac
     4561 atttcctgac tttaacatat ggtggtcatg gatttgaagt atgcagcttt aatgctacaa
     4621 atggaaatgc tgttcacttg tttttaatta caagtccaag tattgtcgct cctggttgtt
     4681 ctggtgttga attttctcct gattcaaaat atctttatgt aaattttaat ggagttgaca
     4741 caagtgaatt gtaccaatat gatatgcaat atgttacgga ttcagctatg tttaaaagtt
     4801 ccgcattgaa aattaatacc taccatacgt ttgatttaca cctcgcaaga gatggaagga
     4861 tttattgcac acctctccag gtcttggatc ctaataagag atattttatc agcgtgattg
     4921 aaaatccttg gatcagagga gacggatgtt ccctcgttac caatgtactt aacttatttc
     4981 caaatcaagt agggtggggt ttccccaaca tcctcgtcga ctacctcctc cgcttcgaat
     5041 ggaccggcga aacgtgccag ggctacccga tccacttcaa gcccaacttc attcctacac
     5101 ctcaaaccat ccaatggaat tttgatgatg ggccgggaag caattcgtgg cagctttcac
     5161 cgacctatac ttttaaaaat cccggtgttc atgaggtgaa ggttgatgtg tggtaccctt
     5221 ccgggagata tgagcacact tccagggaga tagagatctt accgtcaccg caacccgatc
     5281 tcggcccgga tacgttgatc tgtaaagacg ctacgctggt gctcagcgca aactgcgaag
     5341 ccgatttctt ctcctggtcc accggacagt tcggcgtttc atcgataact gtttctgatt
     5401 caggaaccta ctgggtacgt ggccgtttca acaattccgg atgcatggga tatgatacga
     5461 tccatgtcgg ctttcacgct ccaacaatta tcgatgaaac caacctgata atcacacaaa
     5521 ccacctgcaa cggagcaagc ggatcgatta cagggctcat tgctcttggc tattcgcctt
     5581 tcgcgtacag atggaaggac ctctccgagg tccaggttgg aacggatatt gacgtgtccg
     5641 gactcccggc agggcaatat tatctcacaa ttactgacgc taacggttgc gagacagttt
     5701 ccaatgttta caccatcgaa gatgccggga acctgcaggt tctgaacgtt gaacaaaccc
     5761 gcccgcactg cggaaggcct gatggtcaga tcattattca tgcgctttct cccgctggtt
     5821 ctgcattgca gtattcaatc gatgacggcg attcatacca aacggatagt gtgttctccg
     5881 ggctgatcgg tgcgggttac gtggtgcggg ttacggatgg agtaggttgt tttggatttt
     5941 ataatgataa tccggtttta ttggatgata tcccaggtcc tcaggtcgtt gatgtgattg
     6001 ttacggatga aaccgatttt ctggggaatg gcgcgattga gattgtggca acaggatcta
     6061 ctccggttat tttctattca attgacagcg gagcgactta tcaattgaac aatggaacct
     6121 ttaacaatat aggatctggg atttacaatt tacaaatttg ggatgagaat gggtgtgata
     6181 ccacgtttac ggttgagatt caaaatatta tactgacatg gttgcatgcg gttacaggcg
     6241 aaggaggaca ttgtttaggc aatacggcaa tggtgccggt gaatgtcgat aatttcaatg
     6301 ctgttgccga ttttcacctg aagctgggat ataatgccgg taatctggaa tgcgaaggct
     6361 ttgctaatgt taatgttcaa ttgttggata gccttaccgg ttgggttgat gcgattgcag
     6421 gagatatcca tctgacatgg aatggtgaat taccgctgac ttttattcag ccggaaaaag
     6481 tagccgacct ggttttcact accaaaaatc ccgggcaggg agagctttcg tggtacaccg
     6541 gggaaacaga aagctatttc acaaattctt caggcaatcc tatccctgcc gagtttcaga
     6601 ccggcgaggt gaagatctat gaacccccgg agatcatcct ggatcaaagc aaaaccgttt
     6661 ccaccggaca attcgttagc ctgatgagca tcgcggttgg caaccagaac
//
LOCUS       DAOQXS010000091         6569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12090_length_6569_cov_3.400215, whole genome shotgun sequence.
ACCESSION   DAOQXS010000091 DAOQXS010000000
VERSION     DAOQXS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12090_length_6569_cov_3.400215"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKM34_03845"
     CDS             <1..1124
                     /locus_tag="PKM34_03845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ82751.1"
                     /translation="GYTVPTPIQEQAIPHIMVGRDLQGCAQTGTGKTAAFAIPIIQNL
                     YLQKQPVNGIKVLILTPTRELAIQIDQSFAVYGKFTRLTHTVIYGGVSQHHQVIALKK
                     GVDVLIATPGRLLDLIGQGFIDLRRLGTFVLDEADRMLDMGFINDVKKVVVHLPKKRQ
                     TLLFSATMPPEIEKLAQSMLVNPAKAEVTPPATTVDLIEQQLYHTNKINKPDLLLHLL
                     EDKDIRSALVFTRTKHGADKVVRVLLREKVKSAAIHGNKSQNARQAALNSFKEGKLRV
                     LVATDIAARGIDIDDLTHVFNFDLPEVPETYVHRIGRTGRAGNSGTAIAFCTTEEKDD
                     LRGIEKLIGRKVPVVDNHPFPAIAGDTVNKPAPARRRRY"
     gene            1205..2578
                     /locus_tag="PKM34_03850"
     CDS             1205..2578
                     /locus_tag="PKM34_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNQ82752.1"
                     /translation="MDVELLSRIQFAFTVSFHYIYPPLSIGLGLIMVLMEGAYLRTKD
                     KQFEQLARFWTRIFAITFGIGVATGIVMEFEFGTNWATYSRFVGDVFGSALAAEGIFA
                     FALESGFLGVLLFGWNRVKPVVHFIATLGVWIGSMFSAVWIVVANSWQQTPAGFHIVG
                     EGTAARAEITDFWAMVFNPSSVERVLHVWIGALIAGAFLVLSVHAYYLLKGRYVAISK
                     KAFSLALPVAALFSVLQLVTGHSSAEVVAKHQPAKLAAFEGHYNSNEQADLYLFGWVD
                     QSREKAYGPKINGGLSFLLHGNFDTPVPALNDFPEEDVPGAVNAVFQFYHLMVAFGLY
                     FIGLTLFGLFLWWRGKLFKYRWLIWVFVLSVILPQLANQFGWFAAEMGRQPWVVYGLL
                     RTSDALSEVVNAQQVLFSLIMFTLLYILLFGLFIYLLNKKIQAGPYDESMTDNRPSQE
                     AIAGTFN"
     gene            2603..3637
                     /gene="cydB"
                     /locus_tag="PKM34_03855"
     CDS             2603..3637
                     /gene="cydB"
                     /locus_tag="PKM34_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNQ82753.1"
                     /translation="MATILGIDYPTLWFLVVGALFSGYAILDGFDLGAGAWHLFFRKE
                     ESRRIALNAVGPVWDGNEVWLVIGGGALFAGFPVLYASMFSAMYIPFMLFLLVLILRA
                     VSIEFRSKEPMKWWRSLWDITYSISSILMALLLGVVVGNILRGVELGPDHEFRGHWLD
                     FLNPYSLLVGITTVALFMMHGALYLAMKTEGRLFARVNNLLKKSIIFFIITYLLVTVI
                     TFFFLPHLTDNLLENAILLVVPVLAFLSIANIPRLVSKGRYVGAFLFSALTMSFLLIL
                     VAVELYPVMVLSTVDPAYSITVYNAASSEKSLGIMLLLTAIGFPLVITYTVFVYKTFW
                     GKVKLDETSY"
     gene            3789..4169
                     /locus_tag="PKM34_03860"
     CDS             3789..4169
                     /locus_tag="PKM34_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82754.1"
                     /translation="MFSLIPFQAKANVYQKDALDDSPGTGNFALFLRHYQPGFKWVAR
                     PVQPFNPQEHEKKLPEPSAEQFLDKSKRKGISSQGPEPGAPVFTGIFSGYPDHVNNFD
                     PHADLGIDFQYCGIHYRVRPPPAD"
     gene            complement(4374..5816)
                     /locus_tag="PKM34_03865"
     CDS             complement(4374..5816)
                     /locus_tag="PKM34_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HNQ82755.1"
                     /translation="MKWRSLFIVLAFAAFGVTAQSFNKKEPAKLKTSIIITAQNTNYR
                     LSHQMQEQFKPTPQPWESIPCVFVDTAKLFQTFVGIGGALTDASAETFAKLPAKKQEE
                     FLKAYFDREEGIGYSIIRTNMNSCDFSSGSYTYVDEGDISLKSFNIAHDEQYKLPLIK
                     KAMETAGGKLPLFISPWSPPAWMKGNKNMLQGGTLLREYHPAWAEYYVKFIKAYEERG
                     VPVWGLSVQNEPMAKQTWESCIYTAEEERDFIKNFLGPALWNSGLKDKKLIAWDHNRD
                     LVYHRASTILSDPEAAKYVWGIGFHWYESWTGAPTPENVKRVAEAFPNTNLLLTEACN
                     YPFSWDTFDQWNWGENYGAAMISDFNNGAVAWTDWNVLLDETGGPNHVKNFCFAPVHG
                     DTRDGSLHYMNSYYYIGHFSKFIEPGAKRMAVSSNRAQLQTTGFRNPDGKHVVIVMNQ
                     ADTEVKFRLYVGEQAAEMLSLPHSIMTFVF"
     gene            complement(5837..>6569)
                     /locus_tag="PKM34_03870"
     CDS             complement(5837..>6569)
                     /locus_tag="PKM34_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNQ82756.1"
                     /translation="FTAGYPCMEMQPEEQGDFIKNHLGPAFQQAGISTKIIIYDHNWD
                     DTQYPISILNDAAAAQYIAGSAFHAYAGDVSAMSTVHNVHPGKGLYFTEISGGEWAVN
                     FSDNLMWYMDNILIGTTRNWSKTALFWNLALNQANGPQNNGCADCRGVITIYSNNGSV
                     VKNEEYYALAHFSKFVRPGAVRVSLTIPQLLANMGIVSFQNPDGSKVLVACNYSAADR
                     TFSAQQETRHFTATVPAKSVVTIIW"
BASE COUNT         1487 a         1661 c         1671 g         1750 t
ORIGIN      
        1 aaggatatac tgtacctaca cctatccagg aacaagctat tccacacatt atggtaggac
       61 gcgacctgca gggttgcgcc cagaccggga cgggcaaaac agccgccttc gccatcccca
      121 tcatccaaaa tctttattta caaaaacaac ctgtaaacgg tattaaagtc ctgatcctaa
      181 cccctacccg tgagctggcc atccagatcg accagagttt tgcggtgtat ggcaaattca
      241 cccggcttac ccataccgtt atctatggcg gtgtgtcgca acatcaccag gttattgcgc
      301 ttaaaaaagg agtcgacgtg ctgattgcca ccccgggccg gctgctcgac ctgatcggac
      361 agggatttat cgacctccgg cggctcggca cttttgtact cgatgaagcc gaccggatgc
      421 tggatatggg ctttatcaac gatgtaaaaa aagtggttgt ccatctgccg aaaaagaggc
      481 aaacactgct tttctcggct accatgccgc cggaaataga aaaactggcc caatcgatgc
      541 tggtcaaccc ggcaaaagca gaagtgacgc caccggccac cacagtcgac ctgatcgaac
      601 aacaacttta tcataccaac aagatcaaca aacccgacct gttgctccac ctcctggagg
      661 ataaggatat ccggtcggcg ctggtcttta cccgcaccaa acacggcgcc gacaaggtcg
      721 tcagggttct gctgcgggag aaggtcaaat ccgctgccat tcacgggaat aaaagccaga
      781 atgccaggca ggccgccctg aatagcttca aggagggcaa actgagagtg ctcgttgcaa
      841 cggatatcgc ggcccgcggt atcgatatcg acgacctgac ccatgttttc aatttcgatc
      901 tgccggaggt cccggagact tatgttcacc ggatcggccg taccggacgg gccggcaaca
      961 gcggcacagc catcgctttc tgcacaacgg aggagaaaga cgatttgcgc gggattgaaa
     1021 aactgatcgg aagaaaagtc cccgtggttg acaaccatcc atttcccgca attgcgggtg
     1081 atacggtcaa taagccggct ccggcacgtc gcagaaggta ttaatccctt tttaagttta
     1141 cctgccgaaa gataaaaatt gcctattttt atcctttata atatttaaag gataaatttc
     1201 ggttatggat gtagaattgt tatcccggat acagttcgct tttaccgttt cgttccatta
     1261 catttatccg ccgctgagca ttggcctggg gctgatcatg gtgttgatgg aaggggcata
     1321 cctgcggacg aaagacaaac aatttgaaca gctggcccgt ttctggacgc ggatcttcgc
     1381 catcaccttc ggtattggcg ttgcgacggg catcgtcatg gaattcgagt tcggcaccaa
     1441 ctgggccacc tattcgcgct tcgtcggcga tgtattcgga agcgcgctgg cagccgaggg
     1501 gatctttgcc ttcgcgctcg aaagcggttt cctgggcgta ttgctgtttg gctggaaccg
     1561 ggtgaagccg gtggtgcatt tcattgccac gctgggcgtc tggatcgggt cgatgttctc
     1621 ggctgtttgg atcgttgtag ccaacagctg gcagcagacc ccggcaggat tccacatcgt
     1681 cggcgaaggg acagccgccc gtgccgagat caccgatttc tgggcgatgg tcttcaaccc
     1741 gtcgagtgtg gagcgggtac tgcatgtctg gatcggcgcc ctgattgcag gggctttcct
     1801 ggtgttgagc gtacatgcct actacctgct taaaggaagg tacgtggcta tatcgaaaaa
     1861 ggccttctca ctggcactgc cggtggccgc ccttttttcc gttttgcagc ttgtcacagg
     1921 ccacagctcg gcagaggtcg tcgcaaagca ccagccggca aagctggccg cattcgaagg
     1981 gcattacaac agcaacgaac aagccgatct ttacctgttc ggctgggtgg accagtcaag
     2041 ggaaaaggcc tacggaccta agatcaacgg cggactgagc tttcttttgc acggtaattt
     2101 cgacactccg gttcccgctt tgaacgattt cccggaagaa gatgtccccg gggccgtcaa
     2161 tgccgtattc cagttttacc acctgatggt agccttcggg ctatacttta tcggcctgac
     2221 gctgttcggg cttttccttt ggtggcgcgg caagctgttc aaataccgct ggctgatctg
     2281 ggttttcgta ttgtcggtga tcctgcccca gctggcaaac caattcggct ggtttgccgc
     2341 agagatgggt cgccagccct gggtggtcta tgggctgttg cgtacctccg atgcgctttc
     2401 ggaagtagtg aacgcacagc aagtcctttt ttcgctgatc atgttcaccc tgctctatat
     2461 cctgttgttc ggccttttta tttacctgct gaacaaaaag atccaggcgg ggccgtatga
     2521 tgagagcatg acagataacc gcccgtcgca ggaggccatt gccggaacat tcaattaaat
     2581 tacttattaa gctgaacctg atatggcaac aatacttggc atcgattatc ctacgctctg
     2641 gttcctggtc gtcggggcgc tgttttccgg ctatgccatc ctggatggat ttgatctggg
     2701 ggccggggcc tggcatttgt ttttcaggaa agaggagagc cggcgcattg cacttaatgc
     2761 agtcgggccg gtttgggacg gcaacgaagt atggctggtg atcggcggcg gggccctgtt
     2821 tgccggattc cccgtactct acgcctccat gttttcggcc atgtacatcc ctttcatgct
     2881 ctttttgctg gtgctgatcc tccgcgctgt atccatcgaa ttccgcagca aggaaccgat
     2941 gaaatggtgg cgcagcctct gggatatcac ctacagcatc tccagtatcc tgatggcgtt
     3001 gctgctggga gtggttgtcg gaaacatcct ccggggtgtg gagctgggcc cggatcacga
     3061 gttccggggg cattggctgg attttctgaa cccttattcg ttactggttg gaataacgac
     3121 tgtggcacta ttcatgatgc acggagcgct ttacctggcg atgaaaacag aaggccggct
     3181 atttgccagg gtgaacaacc tgctgaaaaa gagcatcatc ttcttcatta tcacctattt
     3241 gctggtcacc gtcattacct ttttctttct cccccatctg accgataacc tcctggagaa
     3301 tgccattttg ctggtggtgc cggttctggc cttcctgagc atagccaaca ttcccaggct
     3361 ggtcagtaaa ggccggtatg tcggcgcctt cctgttttcc gcactgacca tgagtttcct
     3421 gctcatcctg gttgccgttg agctttaccc ggttatggtt ctttcaaccg ttgatccagc
     3481 atacagcatt accgtttaca atgccgcctc gtcggaaaaa tcgctgggga tcatgttgct
     3541 gttgactgca attggatttc cattggtcat aacctacact gtatttgttt ataagacatt
     3601 ctgggggaaa gttaagctgg atgagaccag ttattaatac gaaccgggag gcaatgattg
     3661 gcatttaatg cctattttta cgcctgattt tccggataag agaaacgatg tttgaaatga
     3721 tcagggataa caaaatatta cacatcatcg gcaggtacac acgtatcccg attttgtgtt
     3781 gtttattcat gttttccctg attccttttc aggccaaagc gaatgtttac caaaaagatg
     3841 cccttgatga ttcgcccggt acagggaatt tcgccttatt tctgaggcat tatcagcctg
     3901 gtttcaaatg ggttgcccgg ccggttcagc cttttaaccc gcaagagcat gaaaaaaagc
     3961 ttcctgaacc atctgccgaa cagttcctcg acaagtcaaa aagaaaaggg atcagttcgc
     4021 aggggcccga acccggagcg cctgtgttta ccggaatctt ttcaggttat cctgatcatg
     4081 tcaataattt tgatcctcat gctgatttag gtattgattt ccaatattgc ggaattcatt
     4141 accgggtgcg gcctccgccc gctgattagg aaaaccgggc cgactttcgg ttttccattt
     4201 tccttcacat tctatttatc atacagcatc acctctgcgg agctgtatta ttatttcaac
     4261 aatttaattt tatcaagatg gaagaaaaga aaaaatccag caattgggtc gtattcgcct
     4321 ttttaatcgg cgtggtagta ttgatgatcc tggtaaaggt tttgtttttc tgatcagaaa
     4381 acaaaagtca tgatagaatg aggcaggctg agcatttccg cagcctgctc tcctacatag
     4441 agcctgaatt tcacttcggt atcggcctgg ttcataacga tcaccacatg ttttccgtcc
     4501 ggattcctga accctgtggt ttgcagttgg gcccggttgg atgagacggc catccgtttc
     4561 gccccgggtt cgatgaattt ggagaagtgg ccgatgtaat aataggaatt catatagtgg
     4621 aggctgccgt cgcgtgtatc gccatggacc ggtgcaaagc agaagttttt tacatgattg
     4681 gggcctcccg tttcatcgag gagcacattc cagtcggtcc aggccacagc gccgttgtta
     4741 aaatcggaga tcatagccgc gccgtagttt tcaccccaat tccactggtc gaaagtgtcc
     4801 cagctaaaag ggtaattaca ggcttctgtc agcaggagat tggtattcgg gaaagcctcg
     4861 gccacccgtt tcacgttttc aggcgtcggt gctccggtcc agctttcata ccagtgaaaa
     4921 ccgatgcccc acacatattt ggcggcttcc ggatcgctca ggatggtact ggcccggtga
     4981 tagaccaggt cgcggttatg gtcccaggcg atgagttttt tatccttcag tccgctgttc
     5041 cacaatgcag gtccgaggaa attcttgatg aaatcacgtt cttcttcagc cgtatatatg
     5101 catgattccc aggtttgctt cgccatcggt tcgttctgta cgctaagccc ccacacgggt
     5161 actcccctct cttcgtatgc tttgatgaat ttcacataat attcggccca tgccgggtgg
     5221 tattccctca aaagtgttcc tccctgcagc atatttttat ttcctttcat ccatgccggc
     5281 gggctccagg ggctgatgaa caggggaagt tttcctcctg ccgtttccat agcttttttg
     5341 atcaacggga gcttatactg ttcatcatga gctatattaa acgatttcag gctgatatcg
     5401 ccttcatcca cgtaagtgta actgccgctc gaaaaatcac agctgttcat attggttctt
     5461 atgatggaat aaccaatccc ctcttccctg tcgaaataag ccttcaggaa ctcctcctgc
     5521 tttttggccg gcagtttggc gaacgtctca gcggatgcat cggtcaacgc cccgccgatt
     5581 cccacgaatg tctggaacag tttcgcggta tcgacaaaaa cacaggggat cgattcccat
     5641 ggctggggcg taggtttgaa ctgttcctgc atctggtgtg ataaacggta atttgtgttc
     5701 tgcgcggtga taatgatgga tgtcttcaat tttgcgggtt cttttttatt gaaactctgt
     5761 gctgtcactc caaaagcagc gaaagcgagg actatgaata acgatctcca tttcatcttt
     5821 ttaaaatttt gaatgattac caaatgattg ttacaacaga tttcgcgggg acggttgccg
     5881 taaaatgtct tgtttcctgc tgtgcgctga aggtacggtc ggcagctgaa taattgcaag
     5941 ccacgagaac ttttgaaccg tcgggatttt gaaatgaaac gatgcccata ttggcgagca
     6001 actgcggaat tgttaaagaa acccgcaccg cgccgggccg gacaaatttt gaaaagtggg
     6061 ccagggcata gtactcttca ttcttcacca ccgatccgtt attggaatag atagtgatca
     6121 caccgcggca gtcggcacag ccgttatttt gcgggccgtt ggcctggttc agggcaaggt
     6181 tccagaacag ggccgttttc gaccaattcc gggtcgtccc gattaaaata ttatccatat
     6241 accacatcag gttatcactg aagttaaccg cccattctcc tccggaaatt tcggtaaagt
     6301 agaggccttt tcccgggtgg acattatgga ccgtcgacat ggccgaaaca tcgccggcat
     6361 atgcatggaa agcggagccg gcaatatatt gcgcggctgc cgcatcgttc agaatcgata
     6421 tgggatactg tgtatcatcc cagttatgat catagataat gatctttgtg ctgataccgg
     6481 cttgctgaaa tgcaggtccc aggtggtttt tgatgaaatc gccctgttcc tcaggctgca
     6541 tctccatgca agggtacccg gcggtgaag
//
LOCUS       DAOQXS010000092         6530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12185_length_6530_cov_4.111969, whole genome shotgun sequence.
ACCESSION   DAOQXS010000092 DAOQXS010000000
VERSION     DAOQXS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12185_length_6530_cov_4.111969"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKM34_03875"
     CDS             <1..476
                     /locus_tag="PKM34_03875"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNQ82757.1"
                     /translation="KLNIYLTNAIIAVLITGMIWLTGWKVYLMIQLPVMYFASVAGVY
                     LFYLQHQYEEVIWERDAEWNYKEMALHGSSFFKLPGLLRWFTGNIGYHHIHHLGPTIP
                     NYNLVKCHNENPVFQEVRPINFFRSLRSLSVRLWDEERKKIITFRQLRRYYPAGR"
     gene            complement(526..981)
                     /locus_tag="PKM34_03880"
     CDS             complement(526..981)
                     /locus_tag="PKM34_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNQ82758.1"
                     /translation="MGRGNESFGKKDVRSKKEKKRKEKEQKRLERKENKGATHPDDMI
                     AYVDEFGNITSTPPDRAQRTTVNAEDVAIGVPKAAELNPEDLLRKGIVSFFDESKGFG
                     FIKDLETKQDIFVHINGLLQPVREGNAVTFEVVKGKKGPNAVNVKLKTD"
     gene            1323..1396
                     /locus_tag="PKM34_03885"
     tRNA            1323..1396
                     /locus_tag="PKM34_03885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Ile,seq:cat)
     gene            complement(1492..2694)
                     /locus_tag="PKM34_03890"
     CDS             complement(1492..2694)
                     /locus_tag="PKM34_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82759.1"
                     /translation="MKDYIERDGYIDRIKPFIGKDLIKVLTGQRRVGKSYLLFQIIDE
                     IKSMDNESNIVFINMELFEFAYIKDWNDLITYVNSKSIKDKRNYVFIDEIQDITNFEK
                     ALRHFQASETAEIFCTGSNAGLLSGEIATLLSGRYIEIHVSGLSYNEFLRFQEIEDNN
                     ESLYRYIRYGSLPYLRNLPNDEAVIYDYTRSVYITILYKDIVSRFNIRNTALLENLLR
                     FLMDNIGNLFSAKKISDYLKNQKINLSPKVIIDYIKHFENAFLLYDVKRFGISGKKIF
                     ETGGKFYFEDLGIRHAIGGYRIADIGKIMENLVFKHLQRNYYQVFVGTLGDNEIDFVG
                     RKGNDVLYVQVAYRIDDQKTHEREFGNLLKINDNFRKILVTMDDYIEGPYKGIEHFHI
                     RKFLLMKI"
     gene            2890..3798
                     /locus_tag="PKM34_03895"
     CDS             2890..3798
                     /locus_tag="PKM34_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ82760.1"
                     /translation="MITDSRLKGYSLALLATVAGSTVYIFSKSAFQQVTLPQFGVYWF
                     AFALVWNYLYALVPSAAPKLHRIASGTYNKLIAIGMLELIATTAFYAAIQLAANPAIP
                     SFLRNMEYIFVTLLGVFLLHEKLSKKEAAGAALTFLGAMVISYHRGAAPGSYFTGISG
                     LMLVSTIFYGFRTIMVKSNIHHFSPTVLAINRAYFLLALSIIMLVFLKQSPVIPGRAV
                     INIAIGSFLGPFLTSIGQYSALKYIEATRLAIIQSTTALFVLIGAFLVFGRFPLGYQL
                     VGGALTIAGPMWLILGKRRPFKDGER"
     gene            complement(3836..4168)
                     /locus_tag="PKM34_03900"
     CDS             complement(3836..4168)
                     /locus_tag="PKM34_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HNQ82761.1"
                     /translation="MEWFEKLDGNVIVCDADGIVIYMNETAIRNYEKDGGAALTGKNL
                     LDCHNENSRRKILEIMTTHQKNVYTIEKRGRKKIIYQTPWMDGGVFKGIVELSLEIPS
                     EMPHYKRD"
     gene            complement(4226..4918)
                     /locus_tag="PKM34_03905"
     CDS             complement(4226..4918)
                     /locus_tag="PKM34_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82762.1"
                     /translation="MKKTSSLFLFRFFVLPAILIWTANAAGQDKNLAFGAGIGYCITP
                     VKQHEGYYDWDYKALTGNITPMIAIRYGNKISGVFKASYIQNSFQKHEFSQYGGTSTR
                     DYETTIHQIYADLLFEHTPAGSRGFFYYIGPGFGIPVKVHITDDYFRGAWGPNPAEHT
                     YEEYSVPAEFHLYIQGVLGVGGYIPVKEKNLISIECNFRLGIYKVPVNDFDHVEAKMV
                     AVQFILGYLRRL"
     gene            5020..6147
                     /locus_tag="PKM34_03910"
     CDS             5020..6147
                     /locus_tag="PKM34_03910"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNQ82763.1"
                     /translation="MIIGLLKEMTGENRVALLPEGVAALVKMNATVIVEKGAGASSFA
                     SDADYEKAGAGIESREKVIASSDLVVQINPPDKAELNLLKDSQVLVAVLNPYFNIELV
                     KELAAKNITSFSLDVIPRTTRAQAMDILSSQATAAGYKAVLTAANSLPKFFPMFMTAA
                     GTIKPANVLILGAGVAGLQAVATARKLGAVVYVFDVRAAVKEEVVGLGARFVDVEGAT
                     DDKAAGGYAVEQSEEYKQRQAKAVHDQAVKSDVIICTAQIPGRRAPLLIRKETVEAMM
                     PGSVIIDLAASTGGNCEVTKNGETIVHNGVKVIGNSLFPSDIPTDASRMFGKNVINFL
                     KLIITPEGGLNLNWEDDLVKGTAVTFNKEIVHERVKSVINQ"
     gene            6159..6464
                     /locus_tag="PKM34_03915"
     CDS             6159..6464
                     /locus_tag="PKM34_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNQ82764.1"
                     /translation="MEEVLKFFLEYKDLIFIIILSIFLGIEVISHVPAVLHTPLMSGS
                     NAIHGVVIIGAIIVMGHAENTLSLILGFIAVILGTLNVVGGFVVTDRMLEMFRKKKK"
BASE COUNT         1714 a         1554 c         1410 g         1852 t
ORIGIN      
        1 aaaagctgaa tatttatcta acaaatgcaa ttattgccgt actcataacc ggcatgatct
       61 ggctgacagg atggaaggtt tacctgatga tccagttgcc cgtgatgtat tttgcctcgg
      121 tagccggcgt ttacctgttt tatctgcagc accagtacga agaggtgatc tgggagagag
      181 atgcggagtg gaactataag gaaatggccc tgcacggcag ttcttttttt aagctccccg
      241 ggttattgcg ctggtttaca gggaatatcg gttatcacca tattcatcac ctggggccga
      301 cgatacctaa ctacaatctg gtgaaatgcc ataacgagaa tccggttttc caggaggtca
      361 ggccgattaa ttttttccgg agcctgcggt ctttatcggt cagattgtgg gatgaggagc
      421 gtaaaaagat aattaccttc aggcagttgc gccggtatta ccctgctggc cggtaagcga
      481 ccgaccggtt ttcagtcatt gatcgaaccc tggtgacttt cggattcagt ctgttttcag
      541 cttgacattc accgcattcg gccctttttt ccctttgacc acttcgaatg tcaccgcgtt
      601 gccttcgcgg accggttgga ggagcccgtt gatatggacg aaaatatcct gtttggtctc
      661 cagatctttg ataaacccaa agcctttgct ctcatcaaag aacgatacaa tgcccttgcg
      721 cagcagatct tcagggttta attccgcagc ctttggcaca ccgattgcca catcttcggc
      781 attgacggtg gtccgttgtg ccctgtcggg cggagtggaa gtgatattgc cgaattcgtc
      841 gacgtaagcg atcatatcgt cgggatgggt agccccttta ttctctttcc gctccaggcg
      901 tttttgttct ttttcttttc ttttcttctc tttttttgaa cggacatctt ttttcccaaa
      961 cgattcattt cctcttccca taggtttatt atattgaatt acagatatct aatatgtata
     1021 acggatgctt attgcttata gggtttattt tgtttgaccg caggcataaa ggtaaccttc
     1081 tttattcaaa tggcacgaaa taatccggaa gaatttgaca gagtggcaga aatggaatat
     1141 tttttcaggc ccttacccgc gagtatggat tttcatctga tttttcccta cttttgcact
     1201 cccgaaaatt gacaattcgg aaatttgaag attcgacaat ttaataaaat cgcaacatcc
     1261 ccaaatcgta aatcgtaaat cgtcaaatct ccaaatcgtc aaatcgtcaa atgatgatgt
     1321 tcgggcctat agctcagtcg gttagagcaa ctgactcata atcagtaggt ccctggttcg
     1381 agcccaggtg ggcccacaaa aaagccgctc ctttgtccgt ttaagttttt aataaggcaa
     1441 agagcggctt tttcatttga cgattcgaca atttgcatgg cagatatcat ttcatatttt
     1501 catcaaaaga aatttcctga tatgaaaatg ctcgatcccc ttatacggtc cttcaatata
     1561 atcatccata gtaaccaata tcttcctgaa attatcattg atctttaata aattaccaaa
     1621 ttccctctca tgggtctttt gatcatcaat cctgtaggcc acctggacgt ataaaacatc
     1681 attgcccttt ctgccaacaa aatctatttc attatcgccg agtgttccta caaaaacctg
     1741 ataataatta cgctgcaaat gtttaaacac aagattctcc atgatcttgc ctatatctgc
     1801 aatcctgtat cctccaatag catgtcttat tcctaaatcc tcaaaataaa atttcccgcc
     1861 tgtttcaaat atctttttac cgctgatgcc aaatcttttc acgtcataga gcagaaaagc
     1921 attttcaaaa tgtttgatat aatcgataat gaccttcggg gaaaggttga tcttttgatt
     1981 tttcaggtaa tcgctgatct tttttgccga aaacaaattt ccgatattat ccatcaaaaa
     2041 tctgagcaaa ttctcaagca aagccgtatt cctgatatta aacctgctga caatatcctt
     2101 atacaggatt gtaatatata cactcctggt ataatcatag attacagctt catcatttgg
     2161 aagatttctc aggtaaggta aactgccata cctgatatac ctgtataatg attcgttatt
     2221 atcctcaatt tcctgaaatc ttaaaaactc attataggat aatccggaaa catgaatttc
     2281 aatataccgg ccactaagca atgtggctat ttcacctgag agcaggccag cgttactgcc
     2341 tgtgcagaat atttcagcag tttcggatgc ctgaaaatgc ctcagcgctt tttcaaaatt
     2401 tgtaatatcc tgaatttcat caataaatac atagtttctt ttatccttta ttgatttgga
     2461 gttaacatat gttatcaggt cattccaatc tttaatatag gcgaattcaa acagctccat
     2521 gttaatgaat acaatatttg attcattatc catcgatttg atctcatcaa tgatctggaa
     2581 cagcaagtaa ctcttaccaa ctcttcgctg gccggtaagt actttaatca ggtctttacc
     2641 aataaaaggc ttgatcctat caatatatcc atcacgttca atataatctt tcatttatca
     2701 catgagtaaa ataatcacaa atataatgaa taatttactt atacgtaaat tctttcagag
     2761 ggtttaattt cattatttaa gaacaggtgg cccatagaca tggacgatgg atgattttcc
     2821 atgaacgata gacgaattaa atgtaagatc gtctattgtt tgtgtaattt cgtccatgga
     2881 tccaatgaca tgattaccga cagccgactg aaaggttatt cccttgctct gctggccaca
     2941 gtggccggtt ccacggttta tatcttcagc aagtctgctt ttcaacaggt taccttgcct
     3001 caatttggcg tgtactggtt tgccttcgcg ctggtctgga attatttata cgctctcgtg
     3061 ccgtctgctg ccccaaagct gcaccgcatc gcttccggga catataataa gctcattgcg
     3121 atcggaatgc tggagctaat tgccaccacc gcattttacg cggcaataca actggcggcc
     3181 aacccggcga tcccttcatt ccttcgaaac atggagtata tttttgtcac cttgctgggc
     3241 gtctttttat tgcatgaaaa attatcaaag aaagaggcgg ccggggcggc gctcacgttc
     3301 ctgggtgcga tggtgatcag ctaccacaga ggagccgctc cagggtctta ttttaccggt
     3361 atctcgggtc tgatgctggt ttcgaccatc ttctacggct tcaggaccat catggtcaaa
     3421 agcaatatcc atcatttttc tcccaccgtc ctggctataa acagggctta cttcctgctg
     3481 gccctgtcaa ttataatgct tgtgtttctg aaacaaagcc cggtcattcc ggggcgtgcc
     3541 gtaataaata tagcgatcgg ttcattcctg ggaccgtttc tcacctccat agggcaatac
     3601 agtgccttga aatacattga agcaacgcgg ctggccatca ttcagagcac aacagccctc
     3661 ttcgtcctca ttggcgcttt cctggtcttt ggccggttcc ccctgggata ccagcttgtc
     3721 ggaggggcat tgaccatcgc cgggccgatg tggctgatcc tggggaagag aagacctttc
     3781 aaggatggtg aaagataata ttcatctgcg atcgaagatc ggcgatcggc gatgtttagt
     3841 ctcgcttgta atgcggcatc tcagacggga tctccagcga tagttccacg attcctttga
     3901 acacaccgcc atccatccat ggggtttgat agatgatctt tttgcggccc cgtttttcaa
     3961 tcgtgtaaac gttcttttga tgggtggtca tgatctcgag gatcttccta cgcgaattct
     4021 cgttgtggca atccagcagg tttttgcctg tcagcgcggc cccgccgtct ttttcataat
     4081 tccggattgc cgtttcattc atataaatga caattccatc tgcatcacag acgatgacgt
     4141 tgccgtcgag tttttcaaac cattccatgc taaaaggaaa ttaaagtgct aaagtaataa
     4201 tttggcatat cttacggtca ccgctctata gccgccgcag atacccgaga atgaactgga
     4261 cagccaccat cttagcttca acatgatcaa aatcattaac cgggacctta tatatgccaa
     4321 gcctgaaatt gcactcgatg ctgatcaggt tcttctcttt aaccgggata taaccgccaa
     4381 cacctaaaac accctgtatg taaaggtgaa attctgcagg caccgaatac tcttcataag
     4441 tgtgttccgc gggattgggg ccccacgctc ccctgaaata gtcatccgta atatgcactt
     4501 ttaccgggat gccaaaaccg ggcccgatat aataaaagaa gccccgggat ccggctggcg
     4561 tatgttcaaa caacaggtcg gcataaattt gatggatcgt tgtctcgtaa tcccgtgtac
     4621 tcgtgccgcc atactggcta aactcatgtt tttgaaatga attctgaata tatgaagctt
     4681 taaatacgcc ggaaatttta tttccgtacc gaatggctat catcggggta atattgcctg
     4741 tcaatgcttt ataatcccag tcataataac cctcgtgctg cttgaccggg gttatgcaat
     4801 agcctattcc ggcaccaaag gcaagatttt tatcctgccc ggcggcgttt gcagtccaaa
     4861 tcaagattgc cggcaataca aagaatctga ataaaaacaa ggatgaagtt tttttcatat
     4921 aactcttatt gtttctgtaa atataaagaa taaaacaatt tatggatttc ttttaaatgt
     4981 tctaaatttg cccgattatt aaccctctaa cctcttgcca tgattatagg tttactgaaa
     5041 gaaatgaccg gcgagaaccg tgtggccctc ctgccggaag gcgttgcagc actggtgaag
     5101 atgaatgcca cggtaatcgt cgaaaaaggc gccggggcat catcgtttgc atccgatgcc
     5161 gattatgaaa aggccggtgc cgggatcgaa tccagggaaa aggtcattgc ttcctccgac
     5221 ctggtcgttc agatcaaccc gcccgacaaa gcagagctta atctgctgaa agacagccag
     5281 gtgctggtag ccgtgctgaa cccttatttc aacattgagc tggtaaaaga actggccgca
     5341 aagaacatca cctctttcag cctcgacgtc atcccgcgca ccacccgggc ccaggccatg
     5401 gatattctgt cgtcgcaggc tacggcagcg ggctataaag cggtgcttac cgctgccaac
     5461 agccttccta aattcttccc gatgttcatg acggcagccg gtaccatcaa gccggccaac
     5521 gtgctcatcc tgggagccgg agtggctggg ctgcaggcag ttgccaccgc acgcaagctc
     5581 ggggccgtgg tctatgtctt cgatgtgcgg gcagccgtga aggaagaagt ggtcgggctg
     5641 ggcgcaagat tcgtggatgt ggaaggcgcc accgacgaca aggcagccgg cggctacgcg
     5701 gtggaacagt cggaggagta caagcagcgc caggccaaag cggtccatga ccaggccgta
     5761 aaaagcgacg tgatcatttg cacggcgcag atccccggcc ggcgcgcccc gctgctcatc
     5821 cggaaagaaa ctgtcgaagc catgatgccc ggctcggtga tcatcgacct ggccgcctcc
     5881 acagggggca actgcgaggt caccaaaaac ggcgaaacca tcgttcataa cggtgtcaag
     5941 gtcatcggca actcattgtt cccctcggat atcccgaccg atgccagccg catgttcggc
     6001 aaaaatgtca tcaatttcct caagctcatc attacgccgg aaggaggcct gaacctcaac
     6061 tgggaagatg acctggtgaa aggtacggcc gttactttca acaaggagat tgtccatgaa
     6121 agagtcaaat cagttatcaa ccaataacct aaaatcttat ggaagaagtg ctgaaatttt
     6181 tcctggaata caaggatctg atcttcatca tcatcctgtc gatattcctg ggcattgaag
     6241 tgatctcaca cgtacctgcc gtactgcaca ccccgctgat gtcgggaagc aatgcgattc
     6301 acggcgtggt tattatcggg gccatcatcg tgatggggca tgccgaaaat accctgtcgc
     6361 tcattcttgg ttttattgcc gtgatcctcg gaacattgaa cgtagtaggg ggatttgtgg
     6421 ttaccgacag gatgcttgaa atgttcagga aaaagaagaa ataaggagga cagatcatgg
     6481 aaacaatcac gaaaatcatc gaattctcga accaggtatc cattcttgaa
//
LOCUS       DAOQXS010000093         6488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12289_length_6488_cov_3.723457, whole genome shotgun sequence.
ACCESSION   DAOQXS010000093 DAOQXS010000000
VERSION     DAOQXS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12289_length_6488_cov_3.723457"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1432)
                     /locus_tag="PKM34_03920"
     CDS             complement(89..1432)
                     /locus_tag="PKM34_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ82765.1"
                     /translation="MLIIRYFTLITISILLIFNGVLPAQPLSFNLKEAGEYAVKNSYS
                     VQNARYDLEIARKKVKENLSFGFPQIDGTVDYSYFIALPTSLIPGDFFGQPGEMIEVQ
                     FGTKNNLTAGVTLNQLIFDGRYFIGLQYAKIFEQISAENLEKSEEDIRETVSRTYFSI
                     LVGEESLKILDSTLVILMETLYQTEELYKEGFAERTDFDQLKITVTDLQNMINLQRRQ
                     NELGYKLLKYQMGLDLSQEILLTETLDGLINDAGISALAEQQFNPANHIDYRLISTQE
                     KMQVLSLKNERAAYYPKLNGFVFLQENAQRDSFDFFDIDQPWFMTSSTGVSLKIPVFS
                     SGYRKSRVGQAKLELEKIRLTKNQVQAGLELSALQSRSAFITALENYFRDKQNIGLAL
                     DIYSRTLIKYKEGVATSVELTQQHQQFFNAESKYFQTVFNLLDAKNMLDKALGNY"
     gene            complement(1472..2095)
                     /locus_tag="PKM34_03925"
     CDS             complement(1472..2095)
                     /locus_tag="PKM34_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82766.1"
                     /translation="MDSTFRGIIEKFRDYLYSMNGKAVVTIEQVAVHTGIPEKVIRSC
                     FSNEAELVEKVLDFERHRFEEIFKIHDFEGVNAIDILLTVSKEVAEKFMDISPSVTFV
                     LKRHFPEIYQQHFILRRDFIYEKIKINLSKGISQGMYRDDLSIELVARLYLSRLIDLH
                     NPDFFPAEEFSFDTLFNVMFENFIRGIAKPEGLSYYEKKKKCFKFNA"
     gene            complement(2183..3745)
                     /locus_tag="PKM34_03930"
     CDS             complement(2183..3745)
                     /locus_tag="PKM34_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82767.1"
                     /translation="MKRFALTAILLSGIFLLHAQHQNILITTNASPNEPSIIINPKNT
                     NQLYGGANIASYFYSDDGGYNWQTGTLTSQQYGVWGDPVLLCDTAGAFYFFHLSNPPQ
                     GNWIDRIVCQKVEALGGEWNDGTYMGLDGTKAQDKHWAAVDWKTNNIYVTWTQFDEYG
                     TSDPSKFSNIMFSRSFDGGLSWSPAVQINQVSGDCVDEDNTTEGAVPCVGPEGQIYVS
                     WAGPAGLVFDRSYDQGDTWLDEDIFVSDIGGGWAYDIPGIYRANGLPVTSCDTSSGPY
                     RGTIYINWSDQRNGPGDTDVLLVKSTDGGDTWSDLIRVNDDPPGKHQFFTWMTIDQTN
                     GYLYFVFYDRRNHDNNYTDVYMARSTDGGETFTNFQVSEEYFNPNSNIFFGDYTNITA
                     HDNVIRPVWARCHNNQMSIWTAIVDPDAVGIEEEIAGKPPFSVDRSYPNPFENSTVFS
                     FKLHETAPVRLAVYDSYGRLVEVLADRVILRPGKYTFQFDVRGKNLAAGVYYFSLVSN
                     DKVSRQKVVLAN"
     gene            3809..4327
                     /locus_tag="PKM34_03935"
     CDS             3809..4327
                     /locus_tag="PKM34_03935"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNQ82768.1"
                     /translation="MINLVHGDVKLREFRMEDCHRLVHLANNENISRNLRDGFPHPYT
                     INHAEEFIRNAMMPDPATLFAIEYKEEYVGNIGMVKCKDVYRKSAEVGYFLGEQYWGK
                     GIMTRALGLICEYGFQNLDIVRIHTGVYEHNLASQRVLEKCGFVKEGVFRMAVYKYDR
                     YWNEVRYALIKE"
     gene            4345..5193
                     /locus_tag="PKM34_03940"
     CDS             4345..5193
                     /locus_tag="PKM34_03940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ82769.1"
                     /translation="MAKKRKNYKVDSKEVGLELGLRVYKFFLKSEYLHYGYFKDGLEA
                     DVANLRQAQENYAELIFSHIPEGVKSILDVGCGSGKTAQQLVARGYSVDVVSPGQILT
                     AYARKMLGDSVGFFQCRFENLVTDKKYDLVLFSESFQYIPMDKSIPGALKLLNPGGYI
                     IASDFFGDDPERKSKLGGGHNWAEWLKIKETFPVKTLVEKEITAETSYTIDLVNQLNQ
                     EVLAPVWSSAWALGEDRFPLVIRLVKRLYRKKLAKLENKHFTGQRNGEMFRKYKKYMF
                     YLFQSS"
     gene            5381..>6488
                     /locus_tag="PKM34_03945"
     CDS             5381..>6488
                     /locus_tag="PKM34_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ82770.1"
                     /translation="MNKHISLFNAGRILVILVLCTSLRLAAQDPAAGVSDDALIRQTA
                     LNYIEGFYSGDAARMEKAIHPDLNKATPRDLPQTGRTALNYTTWSGLIEFTRAGIGIL
                     ADSARHIGVNILDMGNDVAVVKAISANFTDYLQVVRINDEWKIINVLFASGLKTPARI
                     KDFDPEKERQEIEKAALAYMNALSGADAGRLSQVIDPEYNRVSLVPVGQTGKVTIRRQ
                     RYENVMENALAGVGKQDEMYRYNRVTVLDAFDGIAAVKCESAGATEQIQMYKGDGQWK
                     VFNSIVRPNTAFTLQQAMTATAGEPMPDFSLPVYGGGHFTLSDYKGKNVMLIFPRGWT
                     GQQWCAYCYYQYMEMEQLERTARIMARNDLQIAFV"
BASE COUNT         1632 a         1500 c         1680 g         1676 t
ORIGIN      
        1 gttcggtttc gggagcgctg cacgaagcga gaaaaacaat taccgctgct aaaaaactgt
       61 atttcatgtt tgaaaatctt ttcaagtttt aataattacc cagggcttta tccagcatgt
      121 ttttcgcgtc aagcaggtta aaaacggtct ggaaatattt actttcggcg ttgaagaact
      181 gctggtgctg ctgggtcagt tccacgctgg tagcaacccc ttctttgtat ttaatgagag
      241 tccggctgta aatatctaga gccaggccaa tgttttgctt atccctgaaa taattctcca
      301 gtgcggtgat gaatgccgat ctggattgta aggctgacag ttccagcccg gcctgaacct
      361 ggttttttgt taaccggatc ttttccagct caagttttgc ctgtccgacc cggcttttcc
      421 ggtaaccgct tgaaaagaca ggtattttca aagaaacgcc ggtggacgag gtcataaacc
      481 agggctggtc gatgtcaaaa aaatcgaaag aatcgcgctg ggcgttttcc tgcaaaaaga
      541 cgaagccatt gagcttggga taataagcgg ctctttcgtt cttcaggctt agcacctgca
      601 ttttttcctg tgttgagatc aaccggtaat cgatgtggtt tgccgggttg aactgctgtt
      661 cggccagcgc gctgatgccc gcatcgttaa tgagtccatc aagtgtttcc gtcagcagga
      721 tttcctggct caggtccagg cccatctggt atttaagcag tttatatccg agttcgttct
      781 ggcgcctctg caaattgatc atattctgca gatcggttac cgtgatcttt agctggtcga
      841 aatcggtcct ctcggcaaat ccctccttgt acaattcttc ggtctgataa agtgtttcca
      901 tcagaatgac cagcgtggaa tcaagaattt tgagcgattc ttcgccgacc aggatgctga
      961 aataggtccg ggaaacagtt tcgcggatat cctcctccga tttctccagg ttttcggcgc
     1021 tgatctgctc aaaaatcttt gcatactgca aaccgataaa ataccgtccg tcgaagatga
     1081 gctggttgag cgtaacacct gccgtcaggt tatttttagt gccgaactgt acctcgatca
     1141 tctcaccggg ctgcccgaag aagtcgcccg gaataagtga tgtaggcagt gcgatgaaat
     1201 aagaatagtc gaccgtaccg tcgatctggg gaaatccaaa ggaaaggttt tctttcacct
     1261 ttttccgggc tatttccagg tcataacggg cattctgaac actgtaactg ttttttacgg
     1321 catattcacc ggcttccttc aggttgaaag aaaggggctg ggctggaaga accccattga
     1381 atatcaatag tatagatatt gttattaatg taaaatatct tataatcaac attaaccgtt
     1441 ttttttgatc gattggcatg ggattggcaa tctatgcatt gaacttaaaa cacttttttt
     1501 tcttctcgta atagctgagc ccttccggtt tggcgatccc gcggataaaa ttttcaaaca
     1561 tcacattgaa aagagtatca aatgaaaatt cttccgccgg gaaaaaatcg gggttatgca
     1621 ggtcgatcag gcggctgagg taaaggcggg caaccagttc gatactgaga tcgtcacggt
     1681 acattccctg gctgatccct ttcgacaggt tgatcttgat cttttcataa ataaaatccc
     1741 gccgtaggat aaaatgctgc tggtatattt ccggaaaatg acgtttcagc acgaatgtca
     1801 ccgaagggga gatatccatg aatttctctg ctacttcctt gctgacagtc aataggatat
     1861 cgatagcatt aactccctcg aaatcatgta ttttaaatat ctcttcaaac cggtgtcttt
     1921 caaaatcaag taccttttcg accaattccg cctcattcga aaaacaggaa cggatcacct
     1981 tttcagggat gccggtatga acggcgactt gttcgatggt aacgacagct ttgccgttca
     2041 tcgaatacag ataatccctg aatttctcaa tgatcccacg aaaggtcgaa tccataagga
     2101 aggttgtttt tgaaagcggc gcaaaattac taattctgct gaagtaacaa aagaaaataa
     2161 atccggttca ctgacgaaaa aattaatttg ccagtacaac tttttgccgg ctgaccttat
     2221 cattgctgac caggctgaag taataaaccc cggcagcgag gtttttgcct ctcacatcaa
     2281 actggaaggt atattttccg ggccggagaa tgacacggtc ggccaggacc tccaccagcc
     2341 ggccatagga atcgtaaacg gccagtctga caggcgctgt ttcgtgcagc ttgaaagaaa
     2401 aaacggtcga attctcgaag gggttgggat agctccggtc gacgctgaat ggtggctttc
     2461 cagcaatctc ttcctcgatg ccgacggcat cgggatcgac gatggcggtc cagatgctca
     2521 tctggttgtt gtggcaccgg gcccaaaccg ggcggatcac gttatcatgt gccgtaatat
     2581 tggtataatc gccaaagaaa atattgctgt tgggattgaa gtactcttcg ctgacctgga
     2641 aattggtgaa cgtttctcct ccatcggtcg agcgggccat gtaaacgtcg gtatagttgt
     2701 tatcatggtt tcggcggtca tagaagacga aatacaggta accgttggtc tggtcgatgg
     2761 tcatccaggt aaagaactgg tgtttgccgg gggggtcgtc gttgacgcgg atgaggtcgc
     2821 tccaggtatc tccgccgtcg gtcgatttga caagcaatac gtcggtatcg ccagggccgt
     2881 tgcgctgatc gctccagttg atgtagatgg ttccgcggta cgggccgctg ctggtgtcgc
     2941 agctcgttac gggaaggccg ttggccctgt agatgccggg aatatcgtag gcccatcccc
     3001 cgccgatgtc ggaaacgaaa atatcctcat ccagccaggt gtcgccctgg tcgtaggaac
     3061 ggtcgaatac cagtccagcc ggtccggccc atgagacata gatctggcct tccgggccaa
     3121 cgcagggcac cgctccttct gtcgtattat cttcatcgac acaatcgccc gaaacctggt
     3181 tgatctgaac cgccgggctc cagctcaggc caccgtcaaa tgaacgggag aacatgatgt
     3241 tactgaattt tgaaggatcg gaagtgccgt attcgtcaaa ctgcgtccag gtaacgtaaa
     3301 tattgttggt tttccagtcg actgcggccc agtgtttatc ctgcgctttg gtgccgtcga
     3361 gccccatgta agtcccgtcg ttccattcac ctcccagggc ttcaactttc tggcaaacga
     3421 tccggtcgat ccagtttccc tgtggcggat tggagaggtg aaaaaagtag aaagcgccgg
     3481 cggtgtcgca cagcaggacc gggtcgcccc agacgccgta ctgctgggat gtcagcgtgc
     3541 cggtttgcca gttgtatcct ccgtcgtccg aataaaaata gctcgcgata ttggcgccac
     3601 cgtaaagttg gttggtgttc ttcgggttga tgatgatcga aggctcgttg ggtgaagcat
     3661 tcgtggtgat caggatattc tggtgctggg cgtgcaacaa aaaaatcccc gataacagga
     3721 tggcagttaa agcaaatctt ttcatgatgg gttcaatgac atcacaaaga tattattttc
     3781 ctacttttga ttgattttaa tgtcgattat gataaacctt gttcatggtg atgtaaaact
     3841 ccgcgaattc cgtatggaag attgccacag gcttgtgcac ctggctaaca atgaaaacat
     3901 cagccgtaac ctgcgcgacg gttttcccca tccatatacg ataaaccatg cggaagagtt
     3961 tatccgcaat gccatgatgc ctgatccggc gactctcttt gccattgagt acaaagagga
     4021 atatgtcggg aatataggca tggtaaaatg caaggatgtt tacaggaaat cggccgaagt
     4081 gggttatttt ctgggggagc aatactgggg aaaaggaatt atgacccggg cgctggggct
     4141 gatctgcgag tacggttttc aaaacctcga tattgtcagg atccatacgg gtgtttatga
     4201 gcacaatctc gcatcgcaaa gggtactgga aaaatgcgga ttcgttaaag aaggtgtttt
     4261 ccggatggct gtttacaaat atgaccggta ctggaatgaa gtcaggtacg ccctcatcaa
     4321 agaataaact atatcattga cttaatggca aaaaaacgaa aaaattacaa ggtcgactcg
     4381 aaagaggtcg gactggaact gggattaagg gtttacaaat tctttctgaa atcggaatat
     4441 ctgcattacg gttattttaa agacggcctg gaagccgatg tggcaaatct ccgccaggcc
     4501 caggagaact atgcggaact gatcttttca catatcccgg aaggggtaaa atcgatcctt
     4561 gacgtcggtt gcggatcagg caaaaccgct caacaactgg tagcccgggg ctattcggtc
     4621 gatgtggtat cacccgggca gatcctgact gcttatgccc gtaaaatgct gggtgattcg
     4681 gtgggattct ttcaatgccg gtttgaaaac ctggtaaccg ataaaaaata cgacctggta
     4741 ctcttcagtg agagcttcca gtatataccg atggacaaat ccatccctgg ggcgcttaag
     4801 ctgctgaatc ccggcgggta tataattgcc agtgattttt tcggcgacga tcccgaaagg
     4861 aaatcaaagt tgggtggcgg gcacaactgg gccgaatggc ttaaaatcaa ggaaactttt
     4921 cccgtgaaaa cgctggttga aaaagagatc acggcagaga catcctacac gatagatttg
     4981 gtcaatcagc taaaccagga ggtgctggcg ccggtctggt cgtcggcatg ggcgctcggc
     5041 gaagaccgtt ttccgctggt aatcaggctg gttaaacgat tgtaccggaa aaaactcgcc
     5101 aaattggaaa acaagcactt caccggccag cgtaacggcg agatgttcag gaaatataag
     5161 aagtacatgt tttatttgtt ccaaagttcc tgagttccgg gttccgggtt ccaggttccg
     5221 ggttccgggt tccaggttcc gggttccagg ttccgggttc cgggttcttt tcttttgaaa
     5281 ttggctgagg tttacctttt gtattgtcat tcgtgccaat tatagaattc cccttctgat
     5341 aatatgatga ttggtttaga tttatgacaa aaatattgtc atgaataaac atatatcgct
     5401 tttcaatgcc ggccggatct tagtgatcct ggtcctttgc acatcgctcc gtttagccgc
     5461 gcaggatccc gctgcagggg tgtcagatga tgccttgatc aggcaaacgg ctttgaatta
     5521 catcgaggga ttttactccg gcgacgctgc ccggatggaa aaagccatcc atcccgacct
     5581 gaacaaggca actccgcggg atttgcccca gaccgggcgc acagcgctca attacaccac
     5641 atggtcgggc ctgattgagt ttacccgcgc cggcatcggc atcctggccg acagtgcccg
     5701 ccatatcggg gtcaatatcc tcgatatggg aaatgatgtg gcggtggtca aggcaatatc
     5761 cgccaatttc accgattact tgcaggtggt caggattaac gatgaatgga aaattatcaa
     5821 cgttttattt gcttcggggc tgaaaacgcc tgcccgcatc aaagatttcg atccggaaaa
     5881 agagagacag gaaattgaga aagcggccct cgcctacatg aatgctttga gtggagccga
     5941 tgccggccgc ctgtcgcagg taatcgaccc ggagtacaac cgggtgagcc tggttccggt
     6001 aggccagacc ggcaaggtga ccatccgcag gcagcgatat gagaatgtga tggagaatgc
     6061 cctggcaggg gtcggcaagc aggacgaaat gtaccgttac aaccgggtta cggtgctgga
     6121 cgcgttcgat gggattgccg ccgtaaaatg tgaatcggcc ggtgccaccg aacagatcca
     6181 gatgtataaa ggcgacggtc aatggaaggt attcaacagc attgtcaggc ccaatacggc
     6241 ttttacgctg cagcaggcca tgacggcaac cgccggggaa ccgatgcccg atttttcgtt
     6301 gccggtttat ggtggcggac atttcacact gtcggactat aaagggaaaa atgtgatgct
     6361 gatctttccg cgcggatgga cgggccagca gtggtgcgct tactgctatt accagtacat
     6421 ggaaatggaa cagcttgaac gaacggcccg catcatggcc cgcaacgacc tgcagatcgc
     6481 tttcgtaa
//
LOCUS       DAOQXS010000094         6488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12290_length_6488_cov_2.948702, whole genome shotgun sequence.
ACCESSION   DAOQXS010000094 DAOQXS010000000
VERSION     DAOQXS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_6488_cov_2.948702"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="murG"
                     /locus_tag="PKM34_03950"
     CDS             <1..1054
                     /gene="murG"
                     /locus_tag="PKM34_03950"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNQ82771.1"
                     /translation="FPAIAIANAIRKKRPETRILFIGAKGRMEMEKVPKAGYEIKALW
                     ISGLKRELSIQNLLFPVKVMFSLVKAIGIIRRFKADLAVGVGGYASGPALRAAGWLGI
                     PTVIQEQNSFPGITNKMLAKKAAKICVAYEGMEKYFPAEKLVVTGNPVRREVILTEGK
                     REEAAEFFGLDHALPTVLVIGGSQGSLSINNTIESLLPGLKENGLQLLWQTGLNFRNR
                     AEECCRAAVYDNVKVADFIYRMDLAYALADAVVSRAGAIAIAELSLVGKAVIFIPLPT
                     AAEDHQMKNARRLELQEAAIVIEDKKSKEELPGVLFNMMNDNILRNKLEKNIAAFGIP
                     DADERIANQILGLISS"
     gene            1051..2409
                     /gene="murC"
                     /locus_tag="PKM34_03955"
     CDS             1051..2409
                     /gene="murC"
                     /locus_tag="PKM34_03955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNQ82772.1"
                     /translation="MNPSFGHIRKIYFLGIGGIGMSALARYFRRKGLSIQGYDKTPTT
                     LTRQLEAEGMVIHYSDDISLIPPDIDLIVYTPAVPESLNEYKFLASSGIRMIKRALAV
                     GELTEGRTTVAVAGTHGKTSISSLIAHILIEAGRPVTALIGGIARNYDSNMVTSGKEE
                     IMVVEADEYDRSFLYLHPDIAVISAIDADHLDIYGSTGSLQQSFSEFAAHIKPGGMLA
                     LREGIEVDLKSTFETVTYSVEGNADISALNPVIREGRQRFDMSLNGRMVRDVALRIPG
                     RHNVENAMAAAAVCVKLGVSPEQIAAAVSTFTGVTRRYDVRLDEPGPVYIDDYAHHPR
                     ELEAFISAVRMMYPGKKLTGMFQPHLYSRTRDLADGFAQSLDLLDEAWLLDIYPAREL
                     PIPGVSSEMILEKMKLPHKKIVSREQALQSLTENRPEIFLTIGAGDIDLLVGKIEKIL
                     RA"
     gene            2406..3197
                     /locus_tag="PKM34_03960"
     CDS             2406..3197
                     /locus_tag="PKM34_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82773.1"
                     /translation="MKKLLNILFLIFLMAAVIILPAFTDLEHHRNVYREFTVEVLNQT
                     DKSLITTSEIHELVRKNFGEIKGSPVSIIDIDKMEKLVDGNPYVSACEVYKTMDCELI
                     MKARVREPLVRIANRQGEQFYLDLNGCAMPLNPAHPSHVTLAGGNISERLMSIDKSEK
                     PLDKFDRSSVLHQIYPVAYAISRDEFLESFIDQIYIHENGEIELVPRVGSQIILLGNG
                     EDAAEKLENLKVFYRKVMRNIDWNTYKTINLKYKNQVVCIKNPTL"
     gene            3194..4603
                     /gene="ftsA"
                     /locus_tag="PKM34_03965"
     CDS             3194..4603
                     /gene="ftsA"
                     /locus_tag="PKM34_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNQ82774.1"
                     /translation="MNKSDIIVGLDIGTTKIAAIVGRRNEHGKIEILGYGNTQSIGVK
                     RGVVSNIENTVQSIRTAVEMAEKSSGVEIRYVNVGIAGHHIKSLQHKGTIIRRDIENE
                     ISQQDIDTLHQNMFNLNMNPGEEIIDVIAQEYIVDGEEDVKQPIGMLGNTLEANFHII
                     IGQTAAAKNIYKCVRKAGLEVVDLILEPIASADAVLGEEEKEAGVVLVDIGGGTTDIA
                     IFQDGIIRHTSVIPFGGEILTEDIKEGCSIIKKHAEELKIRFGSALASENREDEVVAI
                     PGLRGRQPKEITLKNLASIIQARMEEIIEHVYYEIKNSGYEKKLIAGIVLTGGGSLLK
                     HLAQLTEFMTGMDTRIGYPNEHLAPETLDEMASPMYATGIGLVIQGIERQIREQVRSG
                     NKMQDKAPQSREQELPAPAPAPAPSQEQKPEPLPETVDDKPELQEEEEIEEEKPVEKK
                     PRTNGFLKRIQDWFEGDEV"
     gene            4695..>5580
                     /gene="ftsZ"
                     /locus_tag="PKM34_03970"
     CDS             4695..>5580
                     /gene="ftsZ"
                     /locus_tag="PKM34_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ82775.1"
                     /translation="MKEMIQFDMPKNQSSIIKVIGVGGGGSNAVNHMFRQGIKGVDFI
                     ICNTDAQAMESSPVPSKIQLGEKGLGAGSVPAVGREAALNRIEQIREILEKNTQMLFI
                     TAGMGGGTGTGAAPVIAQVARELGILTVGIVTIPFAFEGKKRRTQADEGLLELKKYVD
                     TLVIICNDKLRELCGDLKLSAAFDKADDVLTTAAKGIAEIITVTGYINVDFEDVKTVM
                     KESGKAIMGYAQAEGENRALDAVKGALSSPLLNDNDIEGASNVLLYITSGEEEISMDE
                     VTEITDYIQQEAKSQAEII"
     assembly_gap    5581..5590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5591..6420
                     /locus_tag="PKM34_03975"
     CDS             <5591..6420
                     /locus_tag="PKM34_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82776.1"
                     /translation="DDKLGKSISITLIATGFEPQKKGDAQDNRVVHTLGNQTVAATPM
                     PEMQAIDDSLMNARTFELLRKPAMEETAENEAVEEESFSEPETAEATGEESEMETVSV
                     EAPVMEEKQEMNIPEPEDRTFVFEFQQPEEISREEQEVVKPEYQPIVNRIGRDDQPER
                     PVSGKQDEIRPFEVYVKKPGGETFRDTEKVFTRPSVDLRSQERIERLRNLSLKMHQES
                     VIEELENEPAYVRRNVELKNFTSSGEAHVSKYSLYEDPETRNTEIRSGNSYLHDNVD"
BASE COUNT         1777 a         1664 c         1781 g         1256 t
ORIGIN      
        1 tttcccggca atagccatag ccaatgccat ccgcaaaaag cggccggaaa cccggatcct
       61 ttttatcggt gcaaaagggc gtatggaaat ggaaaaggtg cccaaagcgg gttacgagat
      121 caaagccttg tggatcagcg gccttaagag ggaattgtca atacagaacc tgctttttcc
      181 ggtcaaagta atgttcagcc tggtgaaagc tatcggaatc atccgaagat tcaaggccga
      241 tctggccgtc ggagtgggtg gatacgccag tggcccggcg ctgagagctg cagggtggct
      301 ggggattcct accgtaatcc aggaacagaa ttcatttccc ggcatcacca ataaaatgct
      361 cgctaaaaaa gcggcgaaga tctgtgttgc ttacgaaggg atggaaaagt attttccggc
      421 agaaaaactg gttgtgaccg gcaacccggt ccggagagaa gtcatcctga cggaagggaa
      481 aagagaggag gcggcggagt tcttcggact tgatcatgcg ttgcccaccg tgctcgtcat
      541 cggcggaagc cagggatcct tatccatcaa taatacgatc gaatcccttc tgccgggatt
      601 aaaagaaaac ggcctgcagc tcctctggca gacaggcctg aacttcagga accgggccga
      661 agaatgctgc cgggctgccg tttacgataa tgttaaagtt gccgatttca tctaccggat
      721 ggaccttgcg tatgccctgg ccgacgctgt ggtttcccgc gccggcgcca tcgccattgc
      781 cgagctttca ctggttggaa aagcggtcat ctttattcct cttcccacag cggcagagga
      841 tcaccagatg aaaaatgccc gccggcttga attgcaagaa gccgctatcg tcatcgaaga
      901 caagaaatcg aaggaggagc ttccgggagt tttatttaac atgatgaatg ataatatctt
      961 aagaaataaa cttgaaaaaa atatcgcggc attcggcata ccggatgctg acgaaaggat
     1021 tgctaaccaa atactgggat taatatcgtc atgaacccat cattcggaca tatcaggaaa
     1081 atatattttc tcggcatcgg cggcatcggg atgagcgccc ttgcccgtta tttccgcagg
     1141 aaagggctct ctatccaagg gtacgacaaa accccgacca cccttacccg gcagctcgaa
     1201 gccgaaggga tggtgatcca ttacagcgac gatatctccc tgataccgcc ggatattgac
     1261 cttattgtat acacgccggc tgtgccggaa tcacttaacg aatataaatt cctcgcttcc
     1321 agtgggatcc gcatgataaa acgggccttg gctgttggag aactgacgga aggacggacc
     1381 accgtcgctg tcgcggggac acatggaaag acctccatca gctcgctgat tgcccatatc
     1441 ctgatcgaag caggccggcc cgtcaccgcg ctgatcggcg gaatagcccg gaattacgat
     1501 tccaacatgg tcacctccgg gaaagaggag atcatggtcg tcgaagccga tgagtacgac
     1561 cgctcattcc tgtatctcca tcccgatatc gccgtcatct ccgccattga tgccgaccac
     1621 ctcgacattt acggatcgac gggcagcctg cagcaatcgt tcagcgaatt tgccgctcat
     1681 atcaaaccgg gcggtatgct ggcgctgcgc gaagggattg aggttgatct caaaagcact
     1741 ttcgaaaccg tcacctactc ggtggagggg aatgccgata tttcggctct gaacccggtt
     1801 atccgcgagg gcagacaacg gttcgacatg agcctgaacg gcaggatggt acgcgatgtg
     1861 gccctccgca tcccgggccg tcacaacgtg gaaaacgcga tggccgctgc ggccgtatgc
     1921 gtcaaacttg gtgtgtcgcc ggaacagatt gcggcagcgg tcagcacctt caccggcgtt
     1981 acacgtcgct acgacgtgag gctggatgag cccggcccgg tgtatatcga cgactatgcc
     2041 caccatccca gggaacttga ggcgttcata tccgctgtca ggatgatgta cccgggtaaa
     2101 aagctgaccg gcatgttcca gccccatctg tacagccgga cgcgagacct ggccgacgga
     2161 tttgcgcaaa gccttgacct gcttgatgaa gcatggctgc ttgacattta tcccgccagg
     2221 gaattgccca ttccgggagt atcatccgaa atgatcctcg aaaaaatgaa gctgccgcat
     2281 aagaaaatcg tgagccgcga acaggcactg caatccctta cggaaaacag gcccgaaatc
     2341 ttcctgacca ttggcgcagg cgatatcgac cttctggtcg gaaaaatcga aaaaatactc
     2401 cgggcatgaa gaaactcctg aacatactgt tcctgatctt cctgatggct gccgtcatca
     2461 tcctgccggc tttcaccgac ctggagcacc accggaatgt gtaccgtgag ttcaccgtcg
     2521 aagtcctgaa ccagaccgat aaatcgctca taacaacatc ggagatccat gaactcgtca
     2581 ggaaaaactt tggtgagatc aaaggatcgc ccgtatcgat catcgatatc gataaaatgg
     2641 aaaagctggt tgatggcaat ccctatgtct ctgcctgcga ggtttacaaa acgatggact
     2701 gcgaactaat catgaaagcc agggtcaggg agccgctggt gcggattgca aaccggcagg
     2761 gagagcaatt ctacctcgac ctgaacgggt gtgccatgcc gctgaacccg gcccatccgt
     2821 cgcatgtcac cctggccggc ggaaatattt ccgagcggct gatgtcgatc gataaatcgg
     2881 aaaaaccgct ggacaagttc gacaggagct ccgttctgca ccagatctac cccgttgcat
     2941 atgccatttc ccgggatgaa ttcctggaat catttatcga ccagatctat atccatgaaa
     3001 acggggaaat agagctggta ccccgggtcg gttcacagat catcctgtta ggtaacgggg
     3061 aagatgccgc cgaaaagctg gaaaacctca aggtattcta tcggaaagtg atgcggaata
     3121 tcgattggaa cacctataaa acgatcaatt taaaatataa gaaccaggta gtttgtataa
     3181 aaaacccaac actatgaata agtcggatat tatcgtcgga ttagacatcg gcaccaccaa
     3241 aatcgccgcc attgtagggc gaagaaacga gcacggaaaa attgaaattc tcggatacgg
     3301 caacacccag tcgatcgggg tgaagagagg cgtcgtatcc aacatcgaaa acacggtcca
     3361 gtccatccgt actgctgtcg agatggccga aaaaagttcc ggggtggaga tccggtatgt
     3421 gaatgtcgga attgccggac accacatcaa aagcctgcag cacaaaggga ccatcatcag
     3481 gcgtgatatc gagaacgaga tcagccagca ggacatcgac accctccacc agaacatgtt
     3541 caacctgaac atgaatcccg gggaggagat catcgatgtg atcgcgcagg agtatattgt
     3601 cgacggggaa gaagacgtca agcagccgat cggtatgctg ggtaacacgc tcgaagccaa
     3661 cttccacatc atcatcggcc agacggcggc agccaagaac atttacaagt gtgtcaggaa
     3721 agcggggctg gaggtggtcg acctgatcct ggagccgatc gcatcggccg acgccgtgct
     3781 gggcgaagaa gaaaaagagg ccggcgtcgt gctggtggat atcggcggcg gcacgaccga
     3841 catcgccatc ttccaggacg ggatcatccg gcatacgtct gttatccctt tcggcggtga
     3901 aatcctgacg gaagatatta aagagggttg ttcgatcatt aaaaaacatg ccgaagaact
     3961 gaagatcagg ttcggatcgg cgctggccag cgaaaaccgc gaagacgaag tggttgccat
     4021 ccccgggttg cgcggtcgcc agcccaagga gatcacgctg aagaacctgg ccagcattat
     4081 ccaggcccgc atggaagaga tcatcgagca tgtttattat gagatcaaaa attcgggata
     4141 tgagaagaaa cttattgccg gcattgtgct caccggcggc ggttcgctgc tgaaacacct
     4201 ggctcagctc accgaattca tgaccgggat ggacacccgg atcggctatc cgaacgagca
     4261 tctggcgccg gagacgctgg acgaaatggc cagcccgatg tatgccaccg gtatcggcct
     4321 cgttatccag ggaatcgaac ggcagataag ggaacaggtc aggtcgggga acaagatgca
     4381 ggataaggcg ccacagagca gggaacagga gttgcccgcc ccagcccctg ccccggcacc
     4441 ttcgcaggaa caaaaaccgg aacccctccc cgaaactgtg gatgataagc ctgaactgca
     4501 agaggaagaa gagatcgaag aagaaaaacc ggttgaaaag aaaccgagga ccaacgggtt
     4561 cctgaaacgg atccaggact ggtttgaagg agatgaggta taacatcagt tatcaacaat
     4621 caacctgttt ataattaatc atataaaaaa caaatccaat tcagaaaaga aatattttta
     4681 aattcctgta gaccatgaaa gaaatgatcc aatttgatat gccgaagaac cagtcttcca
     4741 tcataaaagt cataggcgta gggggcggcg ggtccaatgc ggtgaaccac atgttccgcc
     4801 agggaatcaa aggtgtagac tttatcatct gcaacaccga tgcccaggcc atggaatcgt
     4861 cacccgtccc gtcaaagatc cagctaggcg aaaaagggct gggcgccggc tccgtacctg
     4921 cggtcggcag ggaagctgca ctgaacagaa tagaacagat ccgggagatc ctggagaaaa
     4981 atacccagat gctttttatc accgccggca tgggcggcgg aaccggcacg ggcgccgcac
     5041 cggtgattgc ccaggtggcc cgcgaactcg gtatcctgac cgtcggcatc gtgaccatcc
     5101 cctttgcttt cgaaggaaag aaaaggagga cacaggccga tgaaggtctc ctggaattga
     5161 agaaatatgt cgataccctg gtgatcatct gcaacgacaa gctccgggag ctttgcggcg
     5221 acctcaagct ctctgcggct ttcgacaaag ccgacgatgt gctcaccacg gcagccaaag
     5281 gcattgccga gatcatcacc gttaccggct acatcaacgt cgattttgaa gatgtcaaaa
     5341 cggtaatgaa agagagtggc aaagccatca tgggctatgc ccaggccgaa ggcgagaacc
     5401 gcgccctgga tgcggtgaaa ggcgccctct cttctcccct gctcaacgac aatgatatcg
     5461 aaggcgccag caatgtgctg ctctacatca cctcgggcga agaagaaatt tcgatggatg
     5521 aggtgacgga aattaccgac tacatccagc aggaggccaa atcgcaggcc gagatcatct
     5581 nnnnnnnnnn acgacgacaa actgggcaaa agtatcagca tcacgctgat cgccacagga
     5641 tttgaaccgc aaaaaaaagg ggatgcccag gataaccgcg ttgtccacac cctgggtaac
     5701 cagacagtag cggcaacgcc catgcctgaa atgcaggcca tcgacgacag cctgatgaat
     5761 gcccggacct ttgagcttct ccggaaaccg gccatggaag agacggcaga gaatgaagcc
     5821 gttgaagaag aatcgtttag cgagcccgaa accgctgaag cgactggaga agaatccgaa
     5881 atggagaccg tcagcgtcga agcgccggtg atggaggaaa aacaggagat gaatatcccc
     5941 gagccggaag accggacatt cgtttttgaa tttcagcagc ccgaagagat cagccgggaa
     6001 gagcaggaag ttgtgaagcc cgaataccag ccgatcgtca accggattgg ccgggacgat
     6061 cagccggagc gccctgtatc cggcaagcag gatgagatca ggcctttcga ggtctatgtc
     6121 aaaaagccgg gtggcgagac gttccgggat acggaaaagg tctttacgcg tccttcggtc
     6181 gatctgcgtt cacaggagcg gattgagcgg ctgaggaacc tgagcctgaa gatgcaccag
     6241 gagagcgtga tcgaagagct ggagaatgag cccgcttatg tgaggcggaa tgtggaactg
     6301 aagaacttca cctcatcggg cgaagcccat gtttcaaaat attcgctgta cgaagatccc
     6361 gagacccgga atacggagat ccggtcgggg aattcgtact tgcatgataa tgttgactga
     6421 acccggataa taacagagcc atggaccttg agaataggat caatacggat atcaagcagg
     6481 cgatgatg
//
LOCUS       DAOQXS010000095         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12316_length_6478_cov_3.941927, whole genome shotgun sequence.
ACCESSION   DAOQXS010000095 DAOQXS010000000
VERSION     DAOQXS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12316_length_6478_cov_3.941927"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKM34_03980"
     CDS             complement(<1..512)
                     /locus_tag="PKM34_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ82777.1"
                     /translation="MKGKLYLLPSTLGDGSPLSMIPPVVPETIARLRYFIVEDIRSAR
                     RYLKKTNPAIVIDELTFLVLNEHTEPGEIPALLEPMIHGNDAGLLSEAGLPCIADPGS
                     LLVGMAHESGITVVPLPGPSSIFLALMASGFNGQNFAFHGYLPIDKKERIQKIREIES
                     AVYTKDQTQV"
     gene            complement(509..1468)
                     /locus_tag="PKM34_03985"
     CDS             complement(509..1468)
                     /locus_tag="PKM34_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNQ82778.1"
                     /translation="MIPDLQTIQAAAIRIKPYIHRTPVLTSRSINEITGAKIWFKCEN
                     LQKVGAFKSRGACNAVFSLSDEEVTHGVGTHSSGNHAQALARAASIRGCKAYIVMPET
                     SPKVKSDAVRGYGGEITFCKPTLEARESTLAEIIHRTGATEIHPYNDYRIIAGQATAA
                     MELIEATGPPDMIMCPVGGGGLLSGTALATSFLSPSTKVIAAEPAGADDAFCSFTSRT
                     FIPSVNPKTICDGLLTSLGSKTFPIILEKVHRIITVQEETIVRAMMLTWERMKVIIEP
                     SSAVPLAAILENPSVFAGKKIGIIISGGNVSLDKLPWIKNSGS"
     gene            complement(1472..1951)
                     /locus_tag="PKM34_03990"
     CDS             complement(1472..1951)
                     /locus_tag="PKM34_03990"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNQ82779.1"
                     /translation="MKILFVCLGNICRSPLAEAIFTHKAKQLGLNVEANSAGFESFHR
                     GDPADPRSVAVARAHGIDLSDHRARMFTVRDFDHYDRIYVMDRNNYNDVMGVARDSKD
                     EKKVDFMLNLVHPGENRPVPDPWYGGKEGFEKVYHMLDEACENLIHELSHPLPPLHT"
     gene            complement(2171..3610)
                     /gene="dnaA"
                     /locus_tag="PKM34_03995"
     CDS             complement(2171..3610)
                     /gene="dnaA"
                     /locus_tag="PKM34_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ82780.1"
                     /translation="MKRTCTEVWENCLKIIKDNIHYQSYKTWFIPINPVKLENNVLTI
                     QVPSQFFYEWLEEHYVGLLKKTIKKELGSDGRLEYSILMETSYDNPDPLSIKIPATNK
                     KALANPAVSMPIDVNRGASKEIPNPFVIPGLKKIKVNSQLVENYSFENFIEGDCNRLA
                     RSAGFAVAENPGKTAFNPLYLYSNVGLGKTHLAHAIGIQVKNNYPDKTVLYVSTEQFV
                     GQYIESVRNNNQNDFIHFYQMIDVLIIDDIQFLSGKEKTQDVFFHIFNQLHQNSKQLI
                     LTSDKAPVEMKGIEPRLLSRFKWGLAADLQPPDLETRIAILQKKLYRDGIEMPDEVIE
                     YLAYSINTNIRELEGALISIMAQSSLNKKPITLDLAKQMIDKFVKSTSREISIDFIQK
                     IVCDYFNLPVDSISSKTRKREIVQARQLAMYFAKKHTKSSLATIGLHCGNKDHATVLH
                     ACRTVNNLIDTDKQFRIYVEELDKKIKMV"
     gene            complement(3926..4528)
                     /locus_tag="PKM34_04000"
     CDS             complement(3926..4528)
                     /locus_tag="PKM34_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNQ82781.1"
                     /translation="MNTDEYRTLAFEARGLYKEKGSKFIAVALPVSTEEEIRLARERI
                     RKEFHDARHHCFAWRIGEPPYESRYNDDGEPSGTAGKPILGQIQSFELTQILIVVVRY
                     FGGIKLGTGGLIQAYKKASMDALENGQIITKHWKVKVKISFDYLKMNEIMKIIKEEDL
                     MVTGQESGERCHISLEINKYRSLTIYNKFNSVESLEWAVF"
     gene            complement(4531..5385)
                     /locus_tag="PKM34_04005"
     CDS             complement(4531..5385)
                     /locus_tag="PKM34_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ82782.1"
                     /translation="MLFIALSVIQSVLILITFKLFDRFRIDNLQAIVTNYIIAAFFGF
                     LTASTTWSFKLLPHNDWFLTAIFLGFLFIGTFFLFALSSQKIGVAITSVTSKMSLIIP
                     VTVSILLLGESVNLFRIAGIVLAFAAFYFSLKQNEPIRWNSRYTYLPFLVLAGNGLVD
                     TTMKLADHYYISDDVTLFLSVVFSTSFLIGAAILVYRILRRKTILKLKNLAGGVILGS
                     LNFGSTYYMLKAISSFESSVVFPVTNAAIVGISGLAGFFIFREKLTWINWLGITLAIL
                     AILIISKA"
     gene            complement(5385..6422)
                     /locus_tag="PKM34_04010"
     CDS             complement(5385..6422)
                     /locus_tag="PKM34_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ82783.1"
                     /translation="MIRIPAGPWEFVIKEKIYSLLPELTENLAAFLRENRLDYDLFHG
                     HYVDAGIVTVDLARIFGKPAFFTAHSLGAWKRERMDGDPEQMELEFNFNHRIEEEKRI
                     YRTLPGHTVTSRLQLDKLRELYGYEEDNVEIIPPGVNIHKFKPLNPGEPPKKTNLPEK
                     YIFCLSRVDSNKGYDLLLNAFGKVCQKMDDVYLVTGGGSSNPQPREKEVIDMMLRIMK
                     EKGIENKVIFVGHVEEHLMVPYYQNAQFFVMPSIFEPFGMTTQEAMASGIPVIASKFG
                     GINTVLTHEKDGLLIDPKNEDEFAGAMIRLLEETAFRKQLGQKASALIRQNYSWEAMA
                     EKHLHFYKKYV"
BASE COUNT         1549 a         1496 c         1661 g         1772 t
ORIGIN      
        1 aagacctggg tctggtcttt agtgtaaacg gctgactcta tttccctgat cttctgaatc
       61 ctttcttttt tatcgatggg gagataaccg tgaaaagcga aattctgccc gttgaaaccc
      121 gaggccatca gggccaggaa gatcgacgac ggaccgggca gcgggactac cgtaatccct
      181 gattcatggg ccattccgac gagcagagag ccgggatcgg ctatacaggg caggcccgct
      241 tccgataaca ggccggcgtc atttccatgg atcatcggct ccagcagggc aggaatttct
      301 ccgggttcgg tatgttcgtt caagaccagg aaagtcagtt cgtcgatgac gattgccgga
      361 ttggtctttt tcaggtagcg gcgggcgctc cggatatctt ccacaatgaa gtaccggagc
      421 ctggcaatag tttccggcac aaccgggggg atcatcgaaa ggggcgaacc gtcgccaagg
      481 gtagagggaa gcaggtaaag ttttcctttc atgagcctga gtttttgatc cagggcagtt
      541 tatcgaggct gacattaccg cctgaaataa tgatcccaat cttttttccg gcaaaaacgg
      601 acgggttttc caagatggcg gccagtggca cggccgagga gggctcgatg atgaccttca
      661 tcctttccca cgtgagcatc attgccctga caattgtttc ttcctgcacg gtgataatgc
      721 ggtggacttt ttccaggatg atcgggaagg tcttagatcc cagagaggtc agcagcccgt
      781 cgcagatggt tttcgggttc accgaaggga tgaatgtccg gctggtaaag gaacagaagg
      841 catcgtcggc tccggcgggt tctgctgcaa tgactttagt ggaagggctg aggaaagatg
      901 tggccagcgc ggtgccacta agcaggcctc ctcctccgac cgggcacatg atcatatccg
      961 gcgggccggt cgcttcgata agttccatag cagccgtggc ctgtccggct atgatccggt
     1021 aatcgttgta aggatggatc tctgtggctc ccgtccggtg aatgatctca gccagtgtgg
     1081 attcgcgcgc ttccagggta ggtttgcaga aggtaatttc gccgccgtag cctcttaccg
     1141 cgtcactttt tactttcggg gaggtttcgg gcatgacaat ataagctttg catcccctga
     1201 tcgaggctgc acgggccagc gcctgggcgt ggttgcccga cgaatgggtg cctactccgt
     1261 gagtaacctc ttcgtcgcta agcgaaaaaa cggcattaca agcgcctcgt gatttgaatg
     1321 cgccgacttt ctggagattt tcgcatttga accagatctt tgcacccgtt atttcattga
     1381 tactccggga tgtgagcacc ggtgtgcgat ggatgtaggg tttgatcctt atggctgccg
     1441 cttgtatggt ttgaaggtcg ggaatcatgg ttcaggtgtg taagggtggt aaggggtgtg
     1501 agagttcatg aattaaattc tcgcaggctt cgtcgagcat gtggtagact ttttcaaagc
     1561 cttccttgcc gccgtaccat ggatcgggga ccgggcggtt ttctccggga tggaccaggt
     1621 tcagcataaa gtcgactttt ttttcgtcct tgctgtcacg ggccacaccc atcacatcat
     1681 tataattatt acggtccatg acatagatgc ggtcataatg atcgaaatcg cggacggtga
     1741 acatccgggc acggtggtcc gacaggtcga tgccgtgtgc gcgggcgaca gctacggagc
     1801 gcggatcggc cggatcgccc cggtgaaacg attcaaatcc cgcggagtta gcttcaacat
     1861 taagtcccag ttgcttagcc ttatgagtga aaattgcctc agccagagga gaacggcaaa
     1921 tattaccaag acagacaaaa agtatcttca tggatcaaaa ataaagaaat ttggctttaa
     1981 accacatagg catataggac acatagttac acatagagcc gctgtgcgaa atttaattgc
     2041 cttttggaaa cctgttttat ttatttatat cccctgcggg gaatagggta gtgcaaatta
     2101 aattttggaa gaatgcgact gtccaactgt ccaatccctc gcttcgctcg ggacaggctg
     2161 tccaactgaa ctacaccatc ttgatcttct tatccagttc ctccacgtaa atgcggaact
     2221 gcttatcggt gtcgatgagg ttgttgacgg tacggcatgc gtggagaacc gtcgcatggt
     2281 ccttgttgcc gcagtgcagg ccgatggtgg ccaggctcga tttggtgtgc tttttggcaa
     2341 aatacatggc caactggcgt gcctggacga tctcgcgttt ccgggtcttg gagctgatgg
     2401 aatcgaccgg gaggttaaaa tagtcgcata cgatcttctg gatgaagtcg atggaaattt
     2461 cgcgcgaagt gctcttgacg aatttgtcga tcatctgctt ggccaggtca agggtgatcg
     2521 gctttttatt caacgacgac tgggccatga tggagatcag cgctccttcc agttcgcgga
     2581 tgttggtgtt gatgctgtag gccaggtatt cgatcacttc gtcgggcatt tcgatgccgt
     2641 cgcggtagag tttcttctga aggatcgcga tacgggtttc aaggtcaggc ggttgcagat
     2701 cggcggccaa accccactta aagcgggaga ggaggcgtgg ctcgatgcct ttcatctcca
     2761 ccggcgcttt gtcggaggtc aggatcagtt gcttactgtt ctgatgaagc tggttgaaga
     2821 tatgaaagaa cacatcctgc gttttttcct ttcccgaaag gaactggata tcgtcgatga
     2881 taagcacatc gatcatctgg tagaaatgga tgaagtcgtt ctggttatta tttctgaccg
     2941 attcgatgta ttgtccgaca aattgctcgg ttgaaacata gaggaccgtc ttatcggggt
     3001 aattattctt gacctggata ccgatggcat gggccaggtg ggttttaccc aggccgacgt
     3061 ttgaataaag gtaaagcgga ttgaaagccg ttttgcccgg gttttcagca acggcgaatc
     3121 cggcagaacg ggccaggcgg ttacagtctc cttcgatgaa attctcgaat gagtaatttt
     3181 caaccagttg tgaattgacc ttaatttttt tcaggccggg gatgacgaac gggttgggga
     3241 tctcttttga ggcgccccgg tttacatcga tcggcatcga aaccgccggg ttggccagcg
     3301 cttttttatt ggttgccggg attttgatcg aaagagggtc gggattgtca taactggtct
     3361 ccatcaggat gctgtattcc agcctcccgt ctgaacccag ctcctttttg atcgtctttt
     3421 tcagcagccc gacatagtgc tcttcaagcc actcatagaa gaactggctg ggaacctgga
     3481 tagttagcac attattttcc agttttacag gattaatggg aatgaaccat gttttgtagc
     3541 tctggtaatg gatattatcc tttataatct tcaggcaatt ttcccatacc tccgtacaag
     3601 ttcttttcat gctaaaaccc tcgttccgct tgttaaataa tgattttctt ttctctgttt
     3661 attatttccg cttgaaaatc aggtcactca ttcctggctg gtgccctgtg aagttattca
     3721 acaaaagtgt cattaaaaaa gttaaaaaaa aaagtaattt catgcttgac attaaagaat
     3781 ttttagctta tataggcaaa tttgaccccc tcttcttacc taaagttaat ctattgattc
     3841 tctcagtttt ggataaaatc aatttgagtt tgaaaaagta caaaataaaa tcattagatt
     3901 caaagaaaat ctgttaaata ttttgttaaa aaacggccca ttccagtgat tcaaccgaat
     3961 tgaatttatt gtatatggtc agagaacgat atttgttgat ttccaaagag atatggcacc
     4021 tttcacccga ctcctggccg gtcaccatga gatcctcttc tttgattatc ttcattattt
     4081 cattcatttt taaatagtcg aagctgatct tcacttttac tttccagtgc tttgttatga
     4141 tctgtccgtt ttcaagggca tccatactgg cttttttata agcctggatc aatcctcccg
     4201 ttcccagttt aataccgccg aagtatcgta cgactacgat caggatctgg gtaagttcaa
     4261 aggactggat ttgtccgaga atgggttttc ctgcggtacc cgacggctct ccgtcgtcat
     4321 tatatcgcga ttcataaggt ggttcaccaa ttctccatgc aaagcagtga tgacgggcat
     4381 catggaattc tttccggatt ctttccctgg ccagcctgat ctcttcctcc gtggataccg
     4441 gcaaagctac ggctataaat ttgctgccct tctccttgta gaggccgcgg gcttcaaacg
     4501 caagtgtcct gtattcatcc gtattcatac tcaggccttt gatataatta atatggccag
     4561 gatggccagg gtaatgccca gccagttgat ccatgtcagt ttctcacgaa aaataaaaaa
     4621 gccggcaagg cctgatatgc caacgatagc agcattagtc acgggaaaca cgaccgaact
     4681 ttcaaaagag gagatggctt ttaacatata ataggtagag ccgaaattca gcgatcccag
     4741 gatcactccc ccggccaggt tctttaactt caatattgtt tttcgcctta atatcctgta
     4801 aaccagtatt gctgcgccta taagaaagct tgtcgagaat accacggata aaaacaaagt
     4861 aacatcatcg ctgatatagt aatgatcggc cagtttcatg gtcgtgtcta ccaggccgtt
     4921 accggctaaa acaagaaaag gcaggtacgt gtaccttgaa ttccaccgga tgggttcatt
     4981 ttgtttcaga gaaaaataaa aagcggcaaa agccagtacg atgccggcga tccggaaaag
     5041 attgacgctt tcgcccagta acaggatgct gaccgttacg ggtatgataa gcgacatctt
     5101 gctggtcacg gaagtaatgg cgacaccgat tttctgtgag gagagggcaa acagaaagaa
     5161 ggtgccgatg aaaaggaatc ccaggaaaat ggcggtcagg aaccagtcgt tatgcggtaa
     5221 aagtttgaat gaccaggttg tactggcagt gagaaatccg aaaaacgctg caatgatgta
     5281 attggtgaca atagcctgca ggttgtcaat cctgaaccgg tcgaacagtt tgaacgtgat
     5341 caggatcagt accgattgta tgaccgacag ggctataaag agcattaaac atattttttg
     5401 tagaaatgaa ggtgcttttc tgccatggct tcccaactgt aattttgccg gatcagtgcg
     5461 gaggcttttt gcccgagttg tttccggaaa gcggtttctt ccagtagccg gatcatggcg
     5521 ccggcaaatt catcttcgtt tttcggatcg atgagtaagc cgtctttctc gtgcgtcaat
     5581 accgtattga taccgccgaa tttggaggca atgacgggaa ttccgctggc catggcctcc
     5641 tgcgtggtca ttccgaatgg ttcgaaaatg gagggcatga caaagaactg tgcattctga
     5701 taatatggta ccatcaggtg ctcttccaca tggccaacga agatgacttt gttttcaatg
     5761 cctttctcct tcatgatgcg caacatcata tcgatcacct ctttttccct gggctgtggg
     5821 tttgatgagc cgccaccggt cacaaggtaa acatcatcca tcttttggca cacctttccg
     5881 aacgcattta gcaggaggtc atacccttta ttgctgtcga cacggctcag gcaaaaaatg
     5941 tatttttcgg gcaggttggt tttctttggg ggctcgccgg gatttaacgg cttgaattta
     6001 tggatgttca ccccgggcgg gatgatctca acgttatctt cctcataacc atagagttcc
     6061 ctgagtttat cgagctgcaa ccgggaagta acggtatgac ccggcaatgt ccggtagatc
     6121 cttttttcct cttcgattcg gtgattgaaa ttgaactcga gttccatctg ctccggatcg
     6181 ccgtccatcc gttcccgttt ccaggccccc agggagtggg cggtgaagaa tgccggtttg
     6241 ccgaagatgc gggccaggtc gaccgtaaca atccctgcat ccacataatg gccgtggaac
     6301 aggtcgtaat ccagccggtt ttcccggagg aacgcggcca ggttttcggt cagttcgggc
     6361 aggagcgaat agatcttttc cttgatcacg aattcccatg gtcctgccgg tatccggatc
     6421 accctcacat ccgggtaacc gggcaggagc tcgatctgtc tccatggtca caggaacc
//
LOCUS       DAOQXS010000096         6469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12337_length_6469_cov_3.777050, whole genome shotgun sequence.
ACCESSION   DAOQXS010000096 DAOQXS010000000
VERSION     DAOQXS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12337_length_6469_cov_3.777050"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKM34_04015"
     CDS             complement(<1..959)
                     /locus_tag="PKM34_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNQ82784.1"
                     /translation="MIHNEQIIKLLSTLGMEFGDAGQVLKENRIGEGNDGSSLVNAAF
                     RAYYENPWFIPEFVGISFISWSKAMTEEKISGWISRYKTGKEKPAKIKRVGIIMAGNI
                     PMVGLHDLICVLASGNQAVIKASSTDQVLIRAVADYLISQEKGIADQIFFTDEPLRDI
                     DAIIATGSNNSFRYFDYYFSKYQHIIRKNRNSIAVITDQDSPEDFEALGDDIFTYFGL
                     GCRNVSKVYHPEKFDINLLINSFNKFSFIKDHHKYRNNYDYQKSVFLVNGISHLDNGF
                     VLARESTSLISPVSVIHTEPYRSVDSLDLILREQQDSLQCVVAG"
     gene            1099..2172
                     /gene="serC"
                     /locus_tag="PKM34_04020"
     CDS             1099..2172
                     /gene="serC"
                     /locus_tag="PKM34_04020"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNQ82785.1"
                     /translation="MKKHNFFAGPSILPAFTVEKTSEAIKDLNGIGLSLMEISHRSKD
                     FQAIIDEAVALFKELLNIPEGYSVIFLGGGASLQFTMVPYNMLETKAAYLNTGEWATK
                     AIKEAKLFGEVVTVASSEDKLFNYIPRGFNVPNDVDYLHVTSNNTIYGTELFEDFNFG
                     VPLVADMSSDILSRPIDVSKYAMIYGGAQKNLGPAGVTFVIVKNDAVGKVTRKIPTML
                     NYKTHIDKGSLYNTPPVVPIFACLQTLKWLKEIGGISAVEKMNISKSDKLYAEIDRNT
                     LFRPTIPNPADRSRMNVCWVMNDNYKHLEEGFQAFATSQGMVGIKGHRSVGGFRASLY
                     NAMPSSSVDALIACMQEFEKQNM"
     gene            2201..3121
                     /locus_tag="PKM34_04025"
     CDS             2201..3121
                     /locus_tag="PKM34_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HNQ82786.1"
                     /translation="MKKVLVATDKPFAAIAVKGIRQIVDNAGYELLLLEKYTDPADLL
                     KAVEAADAMIVRSDLVTPEVIIAGKNLKIVVRAGAGFDNIDLPASTAAGVVVMNTPGQ
                     NSNAVAELVFGMLVYQIRNHFNGSSGTELMGKKLGLHAYGNVSKNVARIAKGFGMEVF
                     AFDPFVTPDEILKDGVTPVADLKELYSTCQIISLHIPANEKTKKSINFDLLSLMPKNA
                     ILANTARKEVICEDSLQRIFAEREDFRYVSDVAPDCLDSLKEKYSGRFFVTPKKMGAQ
                     TSEANVNAGLAAARQIVEFFEKGDIRFKVN"
     gene            3226..3690
                     /locus_tag="PKM34_04030"
     CDS             3226..3690
                     /locus_tag="PKM34_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ82787.1"
                     /translation="MDAASNDRNLTFFRFEDLRIYHKALDYVAWIHKASLQFPEAQKD
                     NIGIRYNESARAIALNIAEGSARNKSQFIYYLKMAKSSIRETLVLTSALRKCGYLTDK
                     QETESRGYLMELTKMIGALISSLQRGPAGNHHADDEDDMEFPEHPSFKPESY"
     gene            3715..4962
                     /locus_tag="PKM34_04035"
     CDS             3715..4962
                     /locus_tag="PKM34_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNQ82788.1"
                     /translation="MAVIKAFRGLRPPVEIVKELASRPYDVLDSEEARIEARGNDYSL
                     LHVIKPEIDLPPEIDHYAREVYDKALENFKKFQDKKWLVSDEKECLYIYAQTMNNKIQ
                     YGLVACASVEDYMNGIIKKHELTRPDKEEDRKKHVRITNANMEPVFFTYPAHPEIDRI
                     IATHVASFPPVYDFISADGIGHHFWIIEGPETIDQLIRLFDLIPFTYVADGHHRTAAA
                     ALVGNEKKLANPNHTGKEEYNYFLAVHFPDNQLTIIDYNRVVKDLNGLNKEEFLGKIS
                     ANFDLVLKGEDIYKPEHLHEFSMYLDGQWYSLEAKPGTYNDHDPIGVLDVTILSNLVL
                     DEILGIKDLRRDKRIDFVGGIRGLGELKRRVDSGEMAVAFALYPVSMKQLIDIADSGN
                     IMPPKTTWFEPKLRSGLVIHKLD"
     gene            4973..5149
                     /locus_tag="PKM34_04040"
     CDS             4973..5149
                     /locus_tag="PKM34_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ82789.1"
                     /translation="MEIKEIVFKTLKDAREPLKSGEIAEKCGQDKAAVDKAIKALVKE
                     ERIDSPKRCFYQAK"
     gene            5181..5849
                     /locus_tag="PKM34_04045"
     CDS             5181..5849
                     /locus_tag="PKM34_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNQ82790.1"
                     /translation="MTVRHNLEYLLKEIPAGVKLVAVSKTMPAEVILEAYNAGQLRFA
                     ENKSQELTSKQPALPQDIKWHFIGHLQTNKVKYIASFVDMIQSVDSLRLLKEIEKEAS
                     KNKRVIDCLLQFHIATEETKYGLDPDEAMAILNSPEYNECHHIAIRGVMGMASFSDDQ
                     TLVRREFRSLKNIFDRLKNDFFGTDPGFCEISMGMTGDYKIAIEEGSTIVRIGTAIFG
                     SRIL"
     gene            complement(5846..>6469)
                     /locus_tag="PKM34_04050"
     CDS             complement(5846..>6469)
                     /locus_tag="PKM34_04050"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNQ82791.1"
                     /translation="KSGHAIFLDCGTLNFELVPANITGHRLVIINTNKKRGLGDSKYN
                     ERVGECRKAVNYLSKSLNVNSLGEISLIDFRQNEHLITDAIIQKRARHIITENQRVLD
                     AVKALKNNDLSTFGQLMNDSHFSLRDDYEVTGFELDSLVEIMLQQDGVVGARMTGAGF
                     GGCALAFVDEHHLEEFLVNVPVAYQNRTGYRAEVYMPEIGDGVKRIA"
BASE COUNT         1858 a         1438 c         1491 g         1682 t
ORIGIN      
        1 cctgccacca cgcattgcag actgtcctgc tgttctctga gaatgagatc aaggctgtca
       61 accgaccggt aaggttcggt atggatcacc gataccggag aaatcaaaga agtggattcc
      121 cttgccagca cgaaaccatt gtccagatga gaaataccat tgacaaggaa tacagacttc
      181 tggtagtcat aattgttccg gtacttatga tgatccttaa tgaatgaaaa tttgttgaag
      241 ctgttgatta ataagttgat atcaaatttt tcgggatgat atactttcga aacatttcga
      301 caacccagtc caaagtaggt aaagatatca tctcccagcg cttcaaaatc ttcgggagaa
      361 tcctgatctg tgataaccgc aatactattc ctgttttttc ggatgatatg ctgatatttt
      421 gaaaagtaat agtcaaaata cctgaaagag ttattgctgc cggttgcaat gatggcatca
      481 atatccctga gcggttcgtc ggtgaagaag atttggtcag ctattccttt ttcttgtgaa
      541 atcaggtaat ccgcgactgc cctgatcaga acctggtcag ttgacgaagc tttgattacg
      601 gcctggttcc cggaggccag cacgcaaatc agatcatgaa ggcccaccat cggaatattc
      661 ccggccatga ttatccctac tctcttaatt ttggctggtt tctcttttcc tgttttatat
      721 cggctgatcc acccggatat tttctcctca gtcattgctt tcgaccacga aataaaagaa
      781 atacccacaa attcgggaat gaaccaaggg ttctcgtaat aggccctaaa ggccgcatta
      841 accaggctac tcccgtcgtt accctccccg atccggtttt ctttcaggac ctgaccggca
      901 tcgccgaatt ccatcccgag agtggagagt agttttataa tctgttcatt atgaatcata
      961 aatgaaaata tagcggtgca aatttagccc ttatttattt aagaacacga tggtgaagct
     1021 gacgacaaaa attatatctt tgctttttgt taaataatga acaatgacca aatcaaggtt
     1081 ctaaaaacga taactattat gaaaaaacac aacttttttg ccggtccgtc tatccttccg
     1141 gcatttactg tagaaaaaac atcggaagcc attaaagatc tcaatggaat tggattgtcg
     1201 ttaatggaaa tttcacatcg cagcaaagat ttccaggcta ttatcgatga agctgttgcc
     1261 cttttcaaag aattgctcaa cataccggaa ggttactcgg tgattttcct gggaggtggt
     1321 gccagccttc agtttacaat ggttccgtac aatatgctgg agactaaggc cgcttacctg
     1381 aatacgggcg aatgggcgac caaggcaatc aaagaggcca aattattcgg tgaagtggtg
     1441 actgtggctt catctgagga caaactcttc aattatattc cccgcggatt caacgtgccg
     1501 aacgatgtgg attacctcca tgttacatcc aacaatacta tttacggtac tgagctgttt
     1561 gaagatttta atttcggtgt acccctggtc gccgatatgt cgtcggatat cctcagcagg
     1621 ccgatcgatg tatcgaaata tgcgatgatc tatggtggtg cccagaagaa cctgggtcct
     1681 gccggcgtca cgttcgtgat cgtcaaaaac gatgcagtgg gaaaagtgac acgcaaaatc
     1741 ccaacaatgc tgaattataa aacacacatc gataaagggt cgctgtacaa taccccccct
     1801 gttgttccta tcttcgcttg cctccagacg ctgaaatggc tcaaggagat cggtggaatc
     1861 agtgccgtgg aaaagatgaa tatcagcaaa tcagacaaac tttacgctga gatagatcgc
     1921 aacaccctct tccgtccgac cataccgaac ccggccgatc gttcccggat gaacgtttgc
     1981 tgggtgatga acgacaatta caaacacctt gaagaaggat tccaggcttt tgccacatca
     2041 cagggaatgg ttggaatcaa aggacacaga agcgttggcg gattcagggc ttccctctat
     2101 aatgccatgc cttcatcgag tgtcgatgcg ttgatcgcct gtatgcagga gtttgaaaag
     2161 cagaatatgt aagacggaag attaaataaa agatgaaact atgaaaaaag tactggttgc
     2221 taccgataag ccttttgctg ccattgccgt gaagggcatt cgccagatag ttgacaatgc
     2281 tggttatgag ctgcttttac tggaaaaata taccgatcct gccgatttgc tgaaagccgt
     2341 tgaagcggct gatgcgatga ttgttcgcag cgacctggtt acaccggaag tgattatagc
     2401 cggaaaaaat ctgaaaattg tcgtcagggc aggcgccgga ttcgacaata tagatttacc
     2461 tgcttccacc gccgctggcg ttgtagtgat gaatacgccc gggcagaatt ccaatgcagt
     2521 ggccgaactt gttttcggta tgcttgtgta ccagatccgg aatcatttta acgggtcctc
     2581 aggtactgag ttgatgggta aaaagcttgg ccttcacgcc tatggcaatg tcagcaaaaa
     2641 tgtcgcccga attgcgaaag ggtttggaat ggaggttttt gcctttgatc cttttgttac
     2701 accggatgaa atattaaaag atggagttac tcctgttgcc gacctgaaag agctttattc
     2761 gacctgtcag atcatttcgc tgcacattcc ggctaacgag aaaacaaaaa aatcgatcaa
     2821 ttttgatttg ttatccctta tgcccaagaa tgccattctc gccaatacag cgagaaaaga
     2881 ggtaatctgt gaggattccc tgcaaaggat cttcgcggaa cgcgaagatt tcaggtatgt
     2941 ttcagacgtt gcgccggatt gcctggattc attgaaagag aaatattccg gaagattttt
     3001 cgtcaccccg aagaaaatgg gtgcgcaaac ctcggaagcc aatgtgaatg ccgggttggc
     3061 agcagcccgc cagatcgttg aattctttga aaaaggtgac atccgtttca aagtaaatta
     3121 gtgtataaac atatcagggg ctgtttcgat gcagcattta atttaaaatg tggaacagcc
     3181 cctgcctgct tattattctg aaagtaaacc aataaattga ccattatgga tgctgcatca
     3241 aatgatagaa atctcacgtt tttcaggttt gaggacctga ggatttacca caaggccctc
     3301 gactacgtgg cctggatcca caaagccagt ctgcagtttc ccgaggcaca gaaagacaat
     3361 atcgggatca ggtacaatga atcggcccgt gccatagctt tgaatattgc cgaagggtcg
     3421 gccagaaata aaagccagtt tatctactac ctgaagatgg ctaaaagttc tatcagggaa
     3481 acattggtac tgacctctgc attgaggaaa tgcggttatc ttacagataa gcaggagacg
     3541 gaatccagag gttacctgat ggaactgacc aagatgatcg gcgccctgat ttcatccctt
     3601 cagcggggcc cggctggcaa tcaccacgct gatgatgaag atgatatgga atttccggaa
     3661 catccatctt ttaaaccgga atcttattga tcactaacct aactactaaa aactatggct
     3721 gtaattaaag ctttccgggg actcaggccc ccggttgaaa tcgttaaaga actggcatcc
     3781 cggccttatg atgtactgga ttcggaagaa gcgcgcatcg aagcccgggg gaatgattat
     3841 tccctgttgc atgtgataaa gcccgaaata gatctgcccc ctgaaataga ccattatgcc
     3901 cgggaagtct acgacaaagc cctcgaaaat tttaaaaaat tccaggataa aaaatggctt
     3961 gtttcagatg aaaaggaatg cctttatatc tatgcccaaa ccatgaataa caagatccag
     4021 tatggccttg tggcctgcgc ttcggtggaa gattacatga acgggatcat caagaagcat
     4081 gaactgacgc ggcccgataa ggaagaagac agaaaaaagc atgtcaggat cactaatgcc
     4141 aatatggagc ccgtcttctt tacctacccg gctcatccgg agatcgacag gatcatagct
     4201 acacatgttg cttctttccc gcccgtgtat gatttcatat cggcagacgg catcggacat
     4261 catttctgga ttattgaggg tccggaaacg atcgaccagc tgatcaggtt gttcgacttg
     4321 atcccattca cctatgtggc tgacggccac caccggacgg ctgccgcagc cctggtaggc
     4381 aatgagaaaa aactggccaa tccgaatcac acgggaaaag aagaatataa ttatttcctg
     4441 gccgttcatt ttcctgataa ccaattgaca atcattgatt ataacagggt tgtcaaagat
     4501 ctgaacggat tgaataaaga agaatttctc ggtaagatct ccgctaactt tgacctggtg
     4561 ctgaaagggg aagatatcta taaacctgaa catttgcatg aattcagcat gtacctcgat
     4621 gggcaatggt attccttgga agcgaaacca ggaacctaca acgatcacga cccgattggt
     4681 gtcctggatg tgacgattct gtcgaatctg gtcctggatg aaatcctggg aattaaagac
     4741 ctgcggaggg acaagcggat agactttgtc gggggtatcc gtggcctggg cgagctgaaa
     4801 cgccgcgtcg acagtggaga gatggctgtg gcttttgccc tttatcctgt ttctatgaaa
     4861 caactgattg atattgccga cagcgggaat ataatgcctc ccaaaactac ctggttcgaa
     4921 cccaaactgc ggagcggcct ggtgattcat aagcttgatt aattctaatg aaatggaaat
     4981 taaagaaata gttttcaaaa cgctgaaaga tgccagggaa cccctgaaaa gtggtgaaat
     5041 cgctgaaaag tgcggccagg ataaagcagc cgttgataaa gccatcaaag cgctggtaaa
     5101 agaagaacgt atcgattccc cgaaacgctg tttttaccag gcaaagtgaa agcccgtagg
     5161 atcctcatta cctggtgatg atgacagtca ggcataacct ggaatactta ctgaaagaaa
     5221 tccctgccgg cgttaaattg gttgccgttt cgaaaacgat gccggcagag gttatcctgg
     5281 aagcatacaa cgccggacaa cttcgttttg cggaaaacaa atcgcaggaa ctgacttcga
     5341 aacaaccggc ccttccacag gatatcaaat ggcactttat cggtcatctt caaaccaaca
     5401 aggtgaaata tattgcatcc tttgttgata tgatacaatc agttgattca ctgaggcttc
     5461 tgaaggagat cgaaaaggaa gcgagtaaga acaaaagggt tatcgactgc ctgctccagt
     5521 ttcatattgc aacggaagaa accaaatatg gactggatcc ggatgaagcg atggctattt
     5581 taaatagtcc tgaatacaac gaatgtcacc atattgcaat caggggtgtg atgggaatgg
     5641 cctctttctc agatgatcaa acactggtca ggcgggagtt tcgcagcctg aaaaatatct
     5701 ttgaccggtt aaagaacgat ttttttggaa cagatccagg cttttgtgag atcagcatgg
     5761 gaatgaccgg cgattataag atcgctatcg aggaaggcag caccatcgtg aggataggca
     5821 cggcaatttt tggttccaga atactttaag ctattctttt cacgccatcc ccaatttccg
     5881 gcatataaac ttccgcccgg taacccgttc tgttttgata tgcaaccgga acgttcacaa
     5941 ggaattcttc aaggtgatgc tcatcgacga aagccagcgc acaaccgcca aaaccggcgc
     6001 cggtcatacg ggcacccaca accccatcct gctgtaacat aatttctacc aggctatcca
     6061 gctcaaatcc ggtaacctca tagtcgtcac ggagcgaaaa atgagaatcg ttcatcaatt
     6121 gcccgaaagt gctgaggtca ttgttcttta atgctttgac agcatccaga acccgttggt
     6181 tttcggttat gatatgcctg gctctttttt ggatgatggc gtcggtgatc aaatgttcat
     6241 tttgacgaaa atcaatgaga ctaatttctc ccaggctatt gacattcagt gactttgaaa
     6301 gataattcac cgcctttcgg cactccccta ccctttcatt gtattttgaa tcacccagcc
     6361 ctcttttttt attggtattg atgatgacaa gcctgtggcc ggtaatattc gccggaacca
     6421 gctcgaaatt taatgtgccg cagtcaagga aaatggcatg cccggactt
//
LOCUS       DAOQXS010000097         6466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12341_length_6466_cov_3.978163, whole genome shotgun sequence.
ACCESSION   DAOQXS010000097 DAOQXS010000000
VERSION     DAOQXS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12341_length_6466_cov_3.978163"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..872
                     /locus_tag="PKM34_04055"
     CDS             186..872
                     /locus_tag="PKM34_04055"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNQ82792.1"
                     /translation="MLEEFTNIELFPLNVYTVLTNMGVSLACGIVIALVYRFIYKGPS
                     YSATYVNSLVLLSLITTIVIMVIGNNLARAFGLVGAMSIIRFRTAVRDVQDIVYIFFA
                     LTVGMASGVGLYAIAISGTILICLVSVVMVTFNFGAPGRAEYVLQIIYKPSKDTEDRI
                     AGILQKYCRGIRLSNLKSQGENDGIEAFYQFSLRRKYKSTELVEEFSHLSEKVKFNLY
                     FDQDEANIAF"
     gene            979..1338
                     /locus_tag="PKM34_04060"
     CDS             979..1338
                     /locus_tag="PKM34_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82793.1"
                     /translation="MKAKFFFFFILFLGFSAITFAQATTADPVEKKDAPAVVTSPDHA
                     KTGECTGHTTTAKADCKWVDANGDGKCDSCGKTEKECKEACAPKAAATTPKSGCASTC
                     PHAKECGKSTGTLPKKD"
     gene            complement(1497..>6466)
                     /locus_tag="PKM34_04065"
     CDS             complement(1497..>6466)
                     /locus_tag="PKM34_04065"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNQ82794.1"
                     /translation="SSTAVYSFIHNFQSSIVSSSNITINNNTFAASSIQTTGSTHLIY
                     NHNQRLNPSVTSVQNNTVSGTINRTGTSGAFYLYYNANGNGTGTENISGNSFSNITLA
                     GTSSFSGNYSTINANGTQNIFNNTISGITGGTGTFHGMYLGTANTRSVYGNQIYNNTT
                     GGVMNGIFVNSGNPSNIYKNEIYNLTSNSTATDYTMNGILVQAGTNVFIYNNFISDLK
                     TPSSASFEAIRGISITPGQSNSTIGIYYNTIYLNATSTGANFGTAGLYHVSSTDANFT
                     RLDLRNNLIINNSTPNGSGTTVAFRRSAQSTANYAATSNNNILYAGTPASNRLIYWGN
                     TTKQTIEEYREFMGPNRDSISFSELPPFINIATTPYNLRLQDGTTNYCESGAKPITTP
                     LAITDDFDGAVRPGTPDIGADEFAGTSAYVEFPAFLTATSVNSQQIRLEFDSNPDEDD
                     VVIVFNTSGAFSLPAGTPVVGQPLAGGTVVSVGTTSPVIHSGRTPNTAVYYKAYCYNG
                     ANYSLGKIANATPVVTPVTNLTAVCAGQSEIDLGWTKNIYDHDVMVAAHSVYMNGIPV
                     NGTNYNVGDPIPVGGTVIYKGPASGFDHTGLSSWTQFYYKAWSVDIFNYYSSAVTANA
                     ITDADPVTEYGYEQDFDGTWAHDVLAPPNWLVVDVGGSGGFTWIRNSLAERSTPYCVQ
                     GYGSGACNDYLISPPLQLPDTLTQLTWWDKVSVGTNINNYKVLLSTTNNLVSSFTVEL
                     GDYTCVNTAWQQHTLNLTPYRGQTVYIAFYHYYTTQQYEYFVIDDIVIEKNVPGPASI
                     GFPKDEMLTLVDQTLRWNSPVASIPVLGYKLYLDSAPNPTTLVYDGAATSFTPANLAY
                     NTTYYWKVVPYNANGEAYNVPVWLFTTVTSSQLAQSFEAPYFPPVSWEIPSTGWFLNY
                     SNAYHGLQSAYRFTYPALRPKLITPLLEVKAGDELEFYEGTSDKINNFMVICYSTDKS
                     DWDTLGGILDVTRGGWGHRVFDLSALAGGQYYFSFEVWCATNYNAYVYIDHVIGPDVV
                     PILPWAATNPDPPDTASFLPVNLTLGWAPQIWGGIPSGYKVYLDTDPNPTTLIYNGRA
                     ESYAVGPLQLNTTYYWKVVPYNTLGDAQNSVVWSFTTVPVGGVQIGRDTEGFWDLPVY
                     TSYGYNYSQTIYLQSEIDISGKQISKIYYQWNGEEEGTQYKDWVIYMGHTEKTEFTSV
                     TDWVPLSQMTEVFNGEVIIPYNDYDPVWIEIILETPFDYNNIDNLVIAIDENTEGYSW
                     AYGAEFYCTEDPNDRSIQYYSDDDNPDPASPPDADNLVTAYPDIRILCETAATTPVYF
                     CSPASKDFGTVGTFMSSEPKVFKIRNRGVGTLTLTSTALGGTDAGQFQLTDTNSYPVA
                     LLAGEYISMEISFIPTEEGAQTANLLVSHDAAGSPGIVPLGGTGFESRIKTFPFIETF
                     EDNSLTQNQWSQLQETGTSSWTYATGAGDGTITTAHEGLLNARFTDLGLGAVTKLVSP
                     VFDLTGVSNPQLIFWYGQENWSGDQNELKIYYRTAYNQPWVEIFYDNTNRAEWTTDTL
                     SLPNPSLSYQLAFEGIDNYGYANVLDDIYVGPPVTRWTGTISTDWTDPANWTNGVPGV
                     NNAVIINTELYDPVIDVAVTVYAVIVEPGSFITITADGDLTVTGD"
BASE COUNT         1580 a         1562 c         1694 g         1630 t
ORIGIN      
        1 ttttgaggtt aagttcaaag atcagttccc ggtatggctg cggccgatca cgggtcacct
       61 ggggctggtc agaaggtcgg catcgaagta tgttttctgc atcgattccc atcgcctgac
      121 ggagccgtca aaaaaatcgg atatgttcag gtttatgcac tggaacaggt tataattaca
      181 ggaagatgtt agaagaattc accaacatag agctattccc gctgaacgtt tacacggtgc
      241 tcaccaacat gggcgtttca ctggcttgcg ggatcgtcat tgccctggtt taccgcttta
      301 tttacaaagg gccgagctat tcggccacct atgtgaactc gctggttctc ctgagcctga
      361 tcaccacgat cgtgatcatg gtgatcggca acaacctggc gcgtgctttc gggctggtcg
      421 gcgccatgtc gatcatccgg ttccggacgg cggtgcggga tgtgcaggac atcgtctata
      481 tcttctttgc gctaacggta gggatggcct cgggtgtggg gctttatgcc atcgctatct
      541 cgggaacgat cctgatctgc ctggtttccg tggttatggt tacgtttaac ttcggcgcac
      601 ccggccgggc ggagtatgtc ctccagatca tctacaaacc atcgaaagat accgaggacc
      661 ggatcgcagg catcctgcag aaatactgcc gggggatccg gctttctaac ctgaagagcc
      721 agggtgagaa cgacggcatc gaagcgtttt accagttcag cctgcgacgg aagtacaaaa
      781 gcacggagct cgtggaggag ttctcgcacc tttcggaaaa ggtaaaattc aatctctact
      841 tcgaccagga cgaggcgaat atcgcttttt agttccaggt tccaggttcc gggttccggg
      901 ttctaaatct ccaaatctcc aaatctccaa atctccaaat ttctattatc tttgccgttc
      961 atatctaaaa actaacctat gaaagcaaaa ttctttttct ttttcatcct ttttttagga
     1021 ttcagtgcga tcacttttgc ccaggcaaca acggccgatc cggtggaaaa aaaggatgca
     1081 cctgcggtgg taaccagccc tgatcatgca aagaccggag aatgcaccgg gcatacgaca
     1141 acggcgaagg ccgactgcaa atgggttgac gccaatggcg acggcaaatg cgacagttgc
     1201 ggcaagaccg aaaaggagtg caaggaagcg tgtgccccca aggcggctgc caccaccccg
     1261 aaatcgggtt gcgcatcaac ctgcccccat gccaaagagt gcggtaaatc gaccggaacg
     1321 cttcccaaga aggactaagg ctgatttatt tctgccggag ccagactgtc atctacagat
     1381 aatccgttca aatccgttta attcgtgtcg ttcgcgttct ataatggaac acgtataaac
     1441 acggatgtta cggattcaca cggatttgat ttatacatga tgatcctttt tgcgtttcag
     1501 tcacccgtaa cggtaagatc accatctgcc gttatcgtta tgaatgagcc gggttctacg
     1561 attacagcat aaacagtcac cgcaacatcg ataaccggat catataattc cgtattaatg
     1621 atcacggcat tgttcacccc cggaacgccg ttggtccagt tcgccggatc ggtccagtcg
     1681 gtcgaaattg taccggtcca ccgggtaacc ggcggcccga catagatatc atcgagcaca
     1741 ttggcatagc cgtaattatc gatcccttcg aaagccagct gataggataa tgaaggattc
     1801 ggcagcgaaa gtgtatcggt tgtccattcg gccctgttcg tgttgtcata aaaaatttcc
     1861 acccagggtt ggttataggc tgtccggtaa tagatcttca gctcgttctg atcgccgctc
     1921 cagttttcct gaccatacca gaaaataagc tgagggttgg aaacccctgt caggtcaaac
     1981 accgggctga ccagcttggt gacggcgcca aggcccagat cggtaaaccg ggcattcaac
     2041 aacccttcgt gcgcagtggt aatggtgcca tccccggcgc ctgtagcgta agtccacgaa
     2101 ctggttcccg tttcctgaag ttgcgaccat tggttctgtg tcagcgaatt gtcttcaaat
     2161 gtttcaataa aggggaatgt cttgatccgg ctttcgaaac cggtgccgcc gagtggcaca
     2221 attcccggag agccggccgc atcgtgcgaa actaaaaggt ttgcggtctg tgcgccctct
     2281 tcagtgggga taaaactgat ttccatcgaa atatattcgc cggcaagcaa ggcaaccgga
     2341 tacgaattag tgtctgtcag ctggaactga ccggcatctg ttccacccag agcagtactg
     2401 gtaagagtta atgttcctac accccggttc ctgatcttaa atactttcgg ctcagaagac
     2461 ataaacgttc cgaccgtgcc aaaatccttc gatgccgggc tgcagaaata gaccggtgta
     2521 gtcgctgccg tctcacacag gatgcgtata tcgggatacg ccgtcaccag gttatcggca
     2581 tcgggcggac tggcgggatc gggattgtcg tcatcgctgt aatactggat gctcctgtca
     2641 tttgggtctt ccgtacaata aaattcagcg ccgtaagccc atgagtaccc ttccgtattt
     2701 tcatcgatcg cgatgaccag gttatcgata ttattataat caaagggggt ttccaggatg
     2761 atttcgatcc aaaccgggtc ataatcgtta taaggaataa tgacttctcc attaaaaacc
     2821 tctgtcatct gtgacaaagg aacccagtcg gtaacagaag taaattccgt tttttccgtg
     2881 tggcccatat agatcaccca gtccttatat tgggttccct cttcttcacc gttccactgg
     2941 taataaattt ttgagatctg cttgccggag atatcgatct ccgattgcag gtaaatggtc
     3001 tgggaataat tatagccata ggaagtataa acgggcaggt cccagaatcc ctcggtatcc
     3061 cttccgatct gaacgccccc gacaggcacc gttgtaaacg accataccac actgttctga
     3121 gcatctccca gtgtgttata cggcaccacc ttccagtaat aggttgtatt tagctgaagt
     3181 ggaccgacag catatgactc cgccctccca ttgtagatca gtgttgtcgg attgggatca
     3241 gtatcgaggt agactttata gcccgacggg atgccgcccc agatttgcgg ggcccagccc
     3301 agtgtcaggt taacaggcag gaaggacgca gtatccggcg gatccgggtt ggttgccgcc
     3361 cagggtaaaa tgggaaccac atccggcccg atcacatggt cgatataaac ataggcatta
     3421 tagttggttg cgcaccacac ttcaaaggag aagtagtact gaccgccggc cagggcgctg
     3481 agatcgaata cccggtggcc ccaaccaccc cgggtaacat ccaggatgcc tccaagagta
     3541 tcccagtcgg atttgtcagt ggaatagcaa atcaccataa aattgttgat cttgtcggaa
     3601 gttccctcgt aaaattcaag ctcgtcgccg gccttgacct ccagcagcgg ggtgatgagt
     3661 ttcgggcgga gtgcaggata ggtaaacctg tatgccgatt gcaatccatg ataggcattt
     3721 gaataattga ggaaccatcc cgtgctcggg atttcccagc ttaccggagg aaaatagggc
     3781 gcctcaaagc tctgtgcaag ttgagaggag gtcaccgtcg taaataacca gaccggcaca
     3841 ttgtatgctt ctccattggc attgtaaggt acgaccttcc agtaataggt cgtattgtaa
     3901 gccagattgg ctggtgtaaa gctggttgcc gcgccgtcgt aaacgagtgt cgtcgggttt
     3961 ggggcactat ccaaatagag cttataaccc aaaaccggga tggatgccac cgggctgttc
     4021 catctcaggg tctgatcgac cagggtcagc atttcatctt tcggaaaacc aattgaagcc
     4081 ggacctggga cattcttttc gataactata tcgtcgatca caaaatattc atattgctgt
     4141 gtggtataat aatgataaaa ggcaatatac acggtctgtc cccggtaagg cgtgaggtta
     4201 agcgtatgct gctgccatgc tgtgttcaca caggtataat cacccaattc taccgtaaag
     4261 gatgaaacaa ggttattggt cgtggaaagc aacactttgt aattattaat atttgtccca
     4321 accgaaactt tatcccacca ggtaagttga gtgagggtat cgggcaattg caatggaggt
     4381 gagataagat aatcgttgca ggccccgctt ccataaccct gcacacaata cggtgtagat
     4441 ctttccgcca ggctgttcct gatccaggta aagccccctg aaccgccgac atcaaccaca
     4501 agccagttgg gaggcgccag cacgtcgtgg gcccaggtgc cgtcaaagtc ctgctcatag
     4561 ccatattcgg tcaccggatc ggcatcggtg atcgcattgg cggtcaccgc cgacgaataa
     4621 tagttgaata tatcgaccga ccaggccttg taataaaact gggtccatga gctcaagccg
     4681 gtatggtcaa aaccgctggc cgggcctttg tagattacgg ttcctccgac cgggatcgga
     4741 tctcccacat tgtaattcgt tccgttgacc ggaattccgt tcatgtaaac gctgtgcgct
     4801 gccaccatta catcgtgatc atagatgttt ttcgtccagc cgaggtcaat ctccgactgg
     4861 ccggcacaga ctgcggtgag attggtaacc ggcgtaacga ccggagtcgc gttggcaatt
     4921 tttccaagag aataattggc gccattgtaa caataggctt tataatatac tgccgtattc
     4981 ggagtccggc cgctgtggat gacgggagag gtggtgccga ccgaaacgac agttccacca
     5041 gccagcggct gcccgacaac aggagtgccg gccggaagtg agaatgcgcc cgaagtgtta
     5101 aaaacgataa cgacatcatc ctcatccggg ttggaatcaa actcaagcct gatctgctgc
     5161 gaattaacgg aggtagccgt taagaatgcg ggaaattcaa cataggccga tgtaccggcg
     5221 aattcatcgg cgccaatatc aggcgttccg ggcctcaccg ctccgtcgaa atcatcggtg
     5281 atagccaggg gcgtggttat aggcttagcg ccgctttcac aatagttggt cgtgccgtcc
     5341 tgcaggcgaa gattgtaggg ggttgtggcg atattgatga acggaggaag ctcgctgaac
     5401 gagatggagt ctcgattggg gcccatgaat tcgcggtact cctcgatggt ctgtttggtc
     5461 gtattccccc agtatatcag ccggttcgac gccggggtgc cggcgtatag tatattgttg
     5521 ttcgatgttg ccgcataatt ggccgtggat tgggcgcttc ttcggaatgc cacggtagta
     5581 ccgctgccat ttggcgtaga gttattgatg atgaggttgt tgcggagatc cagcctggta
     5641 aaatttgcat cggtactgga gacatggtaa agaccggccg tgccgaaatt ggcccctgtc
     5701 gatgtagcat tcagataaat agtattgtag taaatgccaa tggtagagtt gctctgtccg
     5761 ggggtgatgg aaattcctct gatcgcttcg aaactggcgg atgaaggggt ctttaaatcg
     5821 gatataaaat tattgtaaat gaacacattg gtgccggcct ggaccaggat gccgttcata
     5881 gtatagtcgg ttgcggtcga gtttgatgtt agattataaa tctcattttt ataaatattt
     5941 gaaggattcc ccgaattcac aaagatcccg ttcatcaccc ctccggtggt gttgttataa
     6001 atctggttac cgtaaaccga ccgggtattg gccgttccca ggtacatgcc atggaaagtg
     6061 cccgtcccgc ctgtgatgcc ggatatcgtg ttgttgaata tgttctgggt cccattggcg
     6121 ttgatggtag agtagttccc gctgaaagat gaggtgccgg caagggtaat atttgaaaaa
     6181 ctatttccgg agatattttc agtgcctgtt ccatttccgt ttgcattgta ataaagataa
     6241 aatgcacctg aggtaccggt ccggttgatc gttccggaaa cggtattgtt ctggacgctg
     6301 gtaaccgaag ggttgagccg ctggttatgg ttatagatca gatgagtgct tccggtggtt
     6361 tgaatgctgc ttgccgcgaa agtgttgtta tttatggtga tgttgcttga agatactatt
     6421 gacgactgga aattatggat aaaggaataa acggcggtag atgaag
//
LOCUS       DAOQXS010000098         6406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12491_length_6406_cov_3.992285, whole genome shotgun sequence.
ACCESSION   DAOQXS010000098 DAOQXS010000000
VERSION     DAOQXS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12491_length_6406_cov_3.992285"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..609
                     /locus_tag="PKM34_04070"
     CDS             229..609
                     /locus_tag="PKM34_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82795.1"
                     /translation="MSEKSFGRSFLLLFLSFLIILVYQVLYDHYPVFGEKYRNFAFFE
                     GWVNCSPADSSSAAKDSAALTVAIDTSLYHAAKDSASMPFTGPDSGSVAADLPARLDH
                     FATALHELKSGKRKKVRIAGSAIR"
     gene            606..1715
                     /locus_tag="PKM34_04075"
     CDS             606..1715
                     /locus_tag="PKM34_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82796.1"
                     /translation="MIEGDLIVMTLRNAIQKEFGGEGVGMVPMTSAVAGFRITVKQTF
                     SDNWQEWNVVEQKPPRFPQGLFCTVHTGDVSTSGDPFWVKFKSSNTFKTTSVFHNASL
                     FYGTPSMLRSGREKSMLATEPKAEVYNGDRLIGSFTLDTVGGLQKIDLFTGPLSEFSV
                     YLNSDSAVPYYGISIESDSGVFVDNFASRGNSGIGLWQIDDNMLWRFHDLLRYDLVIL
                     QFGTNAMSPGMTDYNWYGHSMNKVLAKLKKGFSGSSFLLISVADRAMKGDNGMQTDPG
                     VPEMVGVQRRIAAKNGMEFLDLYSAMGGEGTIVKWSELEEPLANKDYTHVNFKGARKI
                     SDIILNFLLQAYREYEPETAPAINDSLQPETDTLK"
     gene            1712..3175
                     /locus_tag="PKM34_04080"
     CDS             1712..3175
                     /locus_tag="PKM34_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ82797.1"
                     /translation="MRIGLVAISFLFAVRIFSQVTLVPAGEYAKIHPFLRPDSNVIIH
                     PEALEQFFIKLACLEHDTLTRLRILHIGDSHIQADFFSGPVRKILQQQFGNAGRGLIF
                     PYKIANTNGPSDIDVFSNTSWTPKRNVFPKIDMPVGISGISLKTGNPFAVMRVSLKAV
                     DSVAQPYDQMTVYAHCSRRSFDLAVSLNASSGVLEKSAVYSENTYYTVKSGDNLGLIA
                     RKFGTTVSQLKAWNGLHSDFIRAGQKLIIKKERQAVQAMSLESFTMLDTLFFSDTLQG
                     QRGRVIRFDQPEKTCYLHMVKTDSIQEEFVLQGILLENADQPGILYSMSGVNGAMYEH
                     YNLSSDFLQQVRDYKPDLVILSLGTNETLGSYFDEEKTQIEMRRMIDSLRNLEPAPDI
                     LLTAPSDNMLRRRYPNKSVIRINELMAKEADSCHVALWNLFSVMGGHGSIDKWYKAGL
                     ARADRIHFTVAGYELQAALLMDALMEAYHKFKDNGLE"
     gene            3162..4622
                     /locus_tag="PKM34_04085"
     CDS             3162..4622
                     /locus_tag="PKM34_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNQ82798.1"
                     /translation="MDWNKIIGVFRYDPASPMLFNSGIFLVMFLGFYGIYVLIRDRRT
                     FLFIYTVLFSLYFYYKASGFYVAIFIASTIINFWLGNRIFLTGNKTGKKAWLVSGIIL
                     NLALLFYFKYTNLFLRSWGTISGSEVTHFDIFLPIGISFFTFQSISYLVDIYKKLLRP
                     PENLLNFSFYATFFPHLVAGPIVRAYVFLPQIYQKLKLTARQIGQAWWLIIAGLIKKA
                     VISDYISINFVDRVFDNPALYSGFENLMAVYGYAIQIYCDFSGYSDIAIGLALLMGFR
                     LPINFNSPYKATSVTDFWRRWHITLSTWLRDYLYIPMGGNRKGKLRTYGNLLMTMLIG
                     GLWHGASWKFVVWGGLHGTALAVEKLFQPLIGDRKSAWLKVAGWILTFHFVCLGWIFF
                     RAADYSIALAVIKAIAGFSGWDIALEIITAEWPVFLVLALGYAGHFFSRRYKLKLEEI
                     FSKSPLPVQALAVSLVIWIILQVASAQVQPFIYFQF"
     gene            complement(4673..5467)
                     /locus_tag="PKM34_04090"
     CDS             complement(4673..5467)
                     /locus_tag="PKM34_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82799.1"
                     /translation="MAKTSKKPVKNRKPAKKKSRSSGRKKSRISKISLGFAAFIILLM
                     AIWIWNPMLYVNLSDSVKRYYRYMTAEESVTYDETAPEAVLRTQKNYGAEIDKLAAQF
                     DLSPEFLKALIILECSGLKNVKPRFERHIYKRLQNVKHGKLARLENIVPADLNNASDD
                     ALKNMAKSWGPFQLMGYKCIWLDIPLSELRGDKALYWGVKWIDMTYGDYIRRGQYKDA
                     FHIHNTGRPYPESGPPKTYDPKYVPNGLMYMKYFKDYAIPVGGKGL"
     gene            5812..>6406
                     /locus_tag="PKM34_04095"
     CDS             5812..>6406
                     /locus_tag="PKM34_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82800.1"
                     /translation="MKRFVSYILLFLLFSGYIATAQGEDIIIDETNLSGTIGPGSYIT
                     DHNLFVDYTDCLTVLPGTTIKFISNTSLTVYGCLISIGTESSGIIYKSSATSVKWNGI
                     SFPGSKALASDPDDILQSKFKYSHFENVTGPGAIYFSNGNAERTLDSISYCSFTGVVN
                     AIHILFINQAGFVINNCDFSDVSNTGITFGNSPAVSGL"
BASE COUNT         1486 a         1618 c         1563 g         1739 t
ORIGIN      
        1 ggcgccgttt atggattggg tttcataggc gcggctattt attacatttc cacagctacc
       61 ggtttctggg tgggcgttct gggtttttta aaagccctgg tatggccggc attcctggtt
      121 tatgaagccc tgaagcacct ggcgatgtaa gagattattc tctcttttca attctttgct
      181 taaatcctgt atttttacag cctcaaattt gcaggcaggt agatttttat gtcagaaaaa
      241 tcattcggca ggtcattctt gttattattc ctttcatttt tgatcatcct ggtttaccag
      301 gtgctatatg accattaccc ggttttcggg gaaaaatacc gcaactttgc cttttttgaa
      361 ggttgggtga attgtagtcc tgccgatagc agctcagcgg ccaaagatag tgcggcccta
      421 accgtggcca tcgatacaag tctgtatcat gcagcaaagg attctgccag catgccattc
      481 acagggcctg attccggttc ggttgctgcg gatttgccgg ccaggctcga ccattttgcc
      541 accgcactgc acgaactgaa atcgggcaaa cgaaaaaaag tgcggattgc tggttcggcg
      601 attcgatgat tgaaggcgac cttatcgtaa tgaccctgcg gaatgccatc cagaaagagt
      661 ttgggggaga gggagtggga atggtgccga tgacctcggc tgtcgccggc ttcaggatca
      721 cggttaaaca gactttcagc gacaactggc aggaatggaa cgtggtcgaa cagaaacctc
      781 cgcgatttcc tcaagggctc ttctgcaccg ttcatacggg tgatgtcagc acaagcggcg
      841 atcccttctg ggtgaaattc aaatccagta acacctttaa aacgacctcc gtttttcaca
      901 atgcctccct gttctacggc acaccatcca tgctccggtc gggcagggag aagtcgatgc
      961 ttgcaacaga gccgaaagcg gaagtataca acggcgaccg gttgatcggt tcatttacac
     1021 tggatacggt gggcggattg cagaaaatcg atctgttcac cggtccgctc agcgagtttt
     1081 ccgtctatct caattccgac agtgctgtac cctattacgg tatcagcatt gaaagcgatt
     1141 cgggggtgtt tgtcgacaat ttcgcctcgc gcggtaattc gggcataggg ctctggcaga
     1201 tcgatgacaa tatgctctgg cgcttccacg acctgctccg gtacgacctg gtgatcctgc
     1261 agttcggcac caatgcgatg tcgcccggca tgaccgatta caactggtac ggccattcca
     1321 tgaacaaagt gctggctaaa ctgaagaaag gcttttcagg cagttctttc ctgttgatat
     1381 cggtggccga ccgggcgatg aaaggagata acggcatgca aaccgacccc ggcgttcccg
     1441 aaatggttgg cgtgcagcgc aggattgctg caaagaacgg gatggagttc ctcgatctgt
     1501 attcggccat gggaggggaa gggacgatcg taaaatggtc ggagctggaa gagcccctgg
     1561 ccaataagga ttatacccat gtgaatttca aaggcgccag gaagatttcc gatattatcc
     1621 tcaatttcct gcttcaggca taccgggagt acgaaccgga aacggcgcct gcaataaatg
     1681 atagtctgca acctgagaca gataccctga aatgaggatc ggactggttg ccatatcgtt
     1741 tttgttcgcc gtcaggatat tttctcaggt gacattggtc cctgccggtg aatatgcaaa
     1801 aattcatccg ttcctgcggc ccgattccaa tgtcatcatc cacccggaag ccctggaaca
     1861 gttttttatc aaactggcct gccttgaaca cgacaccctc acccggctga ggatccttca
     1921 tatcggggac tcgcatatcc aggccgattt tttcagcgga cccgtccgga aaatcctgca
     1981 gcagcagttc ggcaatgccg gccgcggcct catttttcct tataaaatcg ccaataccaa
     2041 cgggccttcc gatatcgatg tcttcagcaa tacttcgtgg acccccaaac gaaacgtttt
     2101 ccctaaaatc gatatgccgg tcgggataag cggcatcagc ctgaaaaccg gaaatccttt
     2161 cgccgtgatg cgcgtaagcc ttaaagcggt cgactccgtc gcacaaccct acgaccagat
     2221 gaccgtatat gcccattgca gccgccgctc cttcgacctg gccgtttcat tgaatgccag
     2281 ctcgggcgtc ctcgagaaat cagcggtcta ttcagagaat acctattaca cggtaaaaag
     2341 cggtgataac ctgggcctga tcgcccggaa atttggcaca accgtctctc agctcaaagc
     2401 ctggaacggt ttgcattccg actttatccg ggctggtcag aaactgatca tcaaaaaaga
     2461 gcgtcaagcg gtacaggcga tgagccttga atcgtttaca atgcttgata cattgttctt
     2521 ctccgacaca ttgcagggcc agcggggcag ggtcatccgg tttgatcagc cggagaaaac
     2581 ctgttacctc catatggtaa aaactgattc catacaggaa gagtttgttc tgcagggtat
     2641 ccttctcgag aacgcggatc agccggggat cctctacagc atgtcgggcg taaacggcgc
     2701 catgtatgag cattataacc tctcttccga ctttttgcag caagtgaggg attacaaacc
     2761 cgacctggtc atcctgtcgc ttgggacaaa tgaaaccctg ggcagctatt tcgacgagga
     2821 aaagactcaa atcgaaatgc gcaggatgat cgactcgctc cggaacctgg aaccggcgcc
     2881 tgatatcctg ctgacggccc cgtccgataa tatgctccgc cgtcgctatc cgaacaaatc
     2941 ggtcatccgg atcaatgaat taatggcaaa ggaagccgac agttgccacg tggctctgtg
     3001 gaacctgttt tcggtcatgg gcggacacgg cagtatcgac aaatggtaca aggccgggct
     3061 ggcccgcgcc gaccgcatcc atttcaccgt tgccgggtat gaactgcagg ccgctctgct
     3121 tatggatgcc ctgatggaag cgtatcacaa attcaaggac aatggactgg aataagatca
     3181 tcggggtttt tcgctacgat ccggcctcgc cgatgttgtt caacagcggg atattcctgg
     3241 tcatgttcct gggattttat ggtatttatg ttctcattcg cgaccgccgg acgttcctct
     3301 ttatttatac cgtactattc tctctctatt tctattacaa agccagcgga ttctatgtcg
     3361 ccatctttat cgcctccacc ataatcaact tctggctggg caaccgtatc tttctgaccg
     3421 gaaataaaac cggcaaaaaa gcctggctgg tatccgggat catcctgaac ctggcccttt
     3481 tgttctattt caaatacacc aacctgtttc tccgatcgtg gggcacgatt agcggaagcg
     3541 aagtcactca cttcgatatt ttccttccca tcgggatctc ctttttcacc tttcagtcga
     3601 tcagctacct ggtcgatatc tacaaaaaat tgcttcggcc cccggagaac ctgctgaact
     3661 tctcctttta tgccacgttt ttcccgcacc ttgtcgccgg ccccatcgtc agggcctatg
     3721 tgttccttcc ccagatctac cagaaactga aactgaccgc ccgacagatc ggacaggcct
     3781 ggtggctcat tattgccgga ctcatcaaga aagcggtgat ttccgattat atcagcatca
     3841 attttgtcga ccgggttttc gacaacccgg ccctgtattc cggcttcgag aacctgatgg
     3901 ctgtttacgg ctatgccatc cagatttatt gcgacttttc cggctattcc gatatcgcca
     3961 tcgggttagc cctgctgatg ggattccgcc ttcccatcaa cttcaacagc ccctacaagg
     4021 ccacatcggt gaccgatttc tggcgccggt ggcacattac gctctcaaca tggctccggg
     4081 attacctgta tattccgatg ggagggaaca ggaaagggaa gctcaggacc tatggcaacc
     4141 tgctgatgac catgctgatt ggcggtttgt ggcacggcgc ttcctggaag ttcgtagtat
     4201 ggggcgggtt gcatggcacg gcgctggcag tggaaaagct ctttcagcca ctgattggcg
     4261 acaggaaatc ggcctggcta aaggttgccg gctggatcct gacctttcac ttcgtttgcc
     4321 tgggatggat cttcttcagg gcggccgact actcgattgc cctggccgtc attaaagcca
     4381 ttgccggatt ttcaggctgg gacattgccc tggagatcat cacggccgaa tggccggtgt
     4441 tcctggtcct ggctttaggc tatgccgggc attttttctc ccggcggtac aaattaaaac
     4501 tggaggagat cttctcaaag tcaccgttgc cggtgcaggc cctggctgtt tcgctcgtga
     4561 tctggatcat cctgcaggtc gccagcgccc aggtgcagcc cttcatctat tttcaattct
     4621 gaaaaaccgg ctaagatcag tgtttattcg tctaattcgt gtcatccgcg ttctaaaggc
     4681 cctttccccc aaccgggatc gcataatcct tgaaatattt catgtacatc aggccattgg
     4741 gaacgtattt cggatcgtag gttttgggtg ggcctgattc agggtaagga cgtcccgtgt
     4801 tatggatgtg gaaggcgtct ttgtattgtc ctcgccggat ataatcgccg taggtcatgt
     4861 cgatccattt cactccccag tagagggcct tgtcgcctct gagctcactg agcggaatat
     4921 ccagccagat gcacttgtaa cccatgagct ggaagggccc ccacgatttg gccatgtttt
     4981 tcagcgcgtc atcggaggcg ttgttcaggt cggccggtac gatattttcc agccgggcaa
     5041 gcttgccgtg ttttacattt tgaagccttt tatagatatg ccgttcgaac cggggtttca
     5101 cgttcttcag gccagagcat tcgaggatga taagcgcttt gagaaattcg ggtgaaagat
     5161 cgaactgggc cgccagttta tcgatctccg cgccgtagtt tttctgggta cgaagaactg
     5221 cctccggggc agtttcatcg taagtgacgc tctcctcggc cgtcatatac cggtaatacc
     5281 gtttcacaga gtcggaaagg ttcacataca gcatcggatt ccagatccat attgccatca
     5341 gcaggatgat gaaagcagca aagcccaggc tgatcttgct gatcctgctt ttcttcctgc
     5401 cggatgagcg gctttttttc tttgctggtt tccggttttt aaccggcttc ttactggttt
     5461 tcgccatgcg ggctcgttgg ggttatcggt tcaccaataa cgcgtttatt ggttgaaatt
     5521 acatattaat gaggtgatag caggctttta tcagaactac ttatcgacaa atataggata
     5581 atagtagatt gtgaccaact tcaggcatac ttagaatctt tctatttttt acccgaaatg
     5641 catggatatt aatcagtttt caatattttt acggggactt ttggaaaaaa atgactatta
     5701 atcggataaa gcattcttta tcaggaagat aatttatatt ttaattgagg tttgctgaaa
     5761 gctgataatg aattaatttt acctgtttca aaaaacccag aaccaaccgt tatgaaaaga
     5821 tttgtcagtt atattctatt gttcttacta ttttctggtt atatagcaac cgctcagggc
     5881 gaggatatta ttattgatga aactaacctt tcgggaacga tcgggcccgg cagttatatt
     5941 accgatcata acctcttcgt cgattatacg gactgtctga ccgttttacc cggaaccacc
     6001 attaaattta tttccaatac ttctttaacg gtttacggat gtctgatttc cattggaact
     6061 gaatcttccg gaattattta caaatcttcg gcaacttccg taaaatggaa tgggatcagt
     6121 ttccccggca gtaaagcttt ggcatctgat cctgatgata tcctgcaatc caaatttaaa
     6181 tattcacatt ttgagaatgt aactggcccg ggagccatct atttttcgaa cggaaatgct
     6241 gaaagaacgc tcgattccat cagctactgc tcttttaccg gcgtggttaa cgctattcac
     6301 atcctgttta tcaatcaggc cggctttgtt atcaacaatt gcgatttctc tgatgtttcc
     6361 aataccggta taacttttgg caacagccct gcagtttcag gcctgc
//
LOCUS       DAOQXS010000099         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12608_length_6355_cov_3.819206, whole genome shotgun sequence.
ACCESSION   DAOQXS010000099 DAOQXS010000000
VERSION     DAOQXS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12608_length_6355_cov_3.819206"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKM34_04100"
     CDS             complement(<1..1129)
                     /locus_tag="PKM34_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82801.1"
                     /translation="MKKSILLCIFLLTFAINGFSDLTTISGTLKNGSGYTVRLMTYND
                     QLSYIRKTLASTVAGEDERFSLSVDISAVTYCWIDIEFQQAELFIQPGQSYEVEIDLA
                     GDPLSMSYYNRRTLDNRIIRDDSMQLNNSIQDFNEIYNDFLLNISDNIRQQNSRVAYE
                     TFMTAVELRFQGISSEYFDNYIRYKSASMQLFMRIKSREKIGTEFLADRPVLFNHLEY
                     MDFFHLYFDKYFVTNGKYFNYSKTYDMINGKAPLAEILDSLAVDPALKQRPAMELLLL
                     CGLKGLYSMPEFSKDRITALVKELTIAGSQPGIRELAANLLVRFKRLKPGSPAPGFAL
                     ETIAGGQKYSLPEFTGKKLYLAFFESDNPASQSELGQISEIY"
     gene            complement(1164..1625)
                     /gene="coaD"
                     /locus_tag="PKM34_04105"
     CDS             complement(1164..1625)
                     /gene="coaD"
                     /locus_tag="PKM34_04105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNQ82802.1"
                     /translation="MERIAVFPGSFDPITKGHESIIRRAMPLFDKIIVAIGENAEKKS
                     FFSLEQRMDWIKQVFVNEPAIEVKSYKGLTVDFCIASKAKFLLRGLRTSADFEFERSI
                     GQINKNLNADVETVFLLTMPEHTALNSSIVRDILRNGGDPSQFVPEAVKFK"
     gene            1739..2380
                     /gene="pyrE"
                     /locus_tag="PKM34_04110"
     CDS             1739..2380
                     /gene="pyrE"
                     /locus_tag="PKM34_04110"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNQ82803.1"
                     /translation="MIYNEEIARTTAKSLLQIKAVKLNPTEPFTWASGIKSPIYCDNR
                     ITLSFHAIRTYIRQQFVHIIREEFGVVEYIGAVATGGIAQGALVAQEFGIPLVYVRSE
                     KKSHGMENKIEGKIEPGKSIVVIEDLVSTGSSSLTAVQALTEAGFIVKGMVAIFSYNL
                     EPARKNFADAKCRLVTLSDYNTLINEALDSNYITDDDMQSLVKWRENPEKWGK"
     gene            2368..2781
                     /locus_tag="PKM34_04115"
     CDS             2368..2781
                     /locus_tag="PKM34_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82804.1"
                     /translation="MGKVTFQSQQATLPVTAGEVYLFFYDLNNLRKLMPEQIINWQSD
                     TDKCSFDIKGMAHLDLQRGETEPDRLVKIISAGDSPIGLEIRGISEDAGENRAVARIE
                     IAAELSPMLQMMVSSPLQNLVKMMAERLEAEMRGS"
     gene            complement(2858..3613)
                     /locus_tag="PKM34_04120"
     CDS             complement(2858..3613)
                     /locus_tag="PKM34_04120"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNQ82805.1"
                     /translation="MMGTIGNEIIRLHEVDSTNRYLMDWINRENVSEGTVVITDFQTA
                     GRGTDGARWESEQSKNLTFSLLLKPVFLAPEAQFYLNKVISLGLADAVNELLPGRTDI
                     RVKWPNDLYIGDRKVAGTLIQNGVKGSRFEFSILGIGLNVNQSRFTDDAANPVSLNQV
                     SGKIFDLPGVLELVLKKIEARYECLKKGDKTCVDADYLKVLYRFNEMARFIVKEKPVE
                     ARITGVNRYGQLILEIPGSKILECDLKEVKFEI"
     gene            3742..4113
                     /gene="rsfS"
                     /locus_tag="PKM34_04125"
     CDS             3742..4113
                     /gene="rsfS"
                     /locus_tag="PKM34_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNQ82806.1"
                     /translation="MTPELYESEQLTELIIQGMEDTKAKKIVILDLQDIDSAVTNYFV
                     ICHGNSKPQLEAIADSVIAKTVKGLKAKPWHKEGFENAEWILLDYVDVVVHIFREDQR
                     EFYQLEKLWADAPITRHDYKE"
     gene            4132..6183
                     /gene="ftsH"
                     /locus_tag="PKM34_04130"
     CDS             4132..6183
                     /gene="ftsH"
                     /locus_tag="PKM34_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNQ82807.1"
                     /translation="MAEENLNKQDKGGAQQPKGNNKSKYFGYWWIGLVFILLLTYLVN
                     WDTGTAKKATWGEVKEMLATGDIERIVLVNKEAAEFYLKNEKLDDPKYKEIKETVAAK
                     GPHYTYNITSPESFERMVAEAQEGIADPIYIENDVRRNWTGEVLSWIVPIAILIIIWL
                     VIMRMMSRNAGGPGGQIFNIGKSKAQLFDKTTSVSINFNDVAGLEEAKLEVKEVVDFL
                     KNPSKYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVG
                     ASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGKNQITGANDERESTLNQLLTEMDGFS
                     TNSGVIIMAATNRADILDRALLRAGRFDRQINVELPDLDERRAIFAVHTKNLKFDKTS
                     VDLEWLARQTPGFSGADIANVANEAALIAARNNKKIIEKQDFIDAVDRIIGGLERRSK
                     IISQEEKRVIAFHESGHAAVSWLLPHAHPLVKVTIVPRGKALGAAWYLPEERQITTFD
                     QMFDEMTATLGGRAAEEIIFGKVSTGALNDLERVTKQAYNMVVFFGLNEKLGNISYYD
                     STGQQEYMFNKPYSEKTAEIIDQEIGKLIDQAYKRAVEILRTHKDKLEALASILLEKE
                     VIFTEDLETIFGKKIKHVGHEVVLADPNGNGHVEAPAVVEEAKPKKRGRKPGSVGQLD
                     S"
BASE COUNT         1695 a         1546 c         1651 g         1463 t
ORIGIN      
        1 catatatctc tgaaatctgg cccagttccg actggctggc cgggttatcc gactcgaaga
       61 atgccaggta aagttttttc ccggtaaatt cgggcaagga atatttctga ccgccggcga
      121 tggtttccag ggcaaaaccg ggcgcaggtg aaccgggctt gagtctttta aaacggacga
      181 gcaaattagc cgcaagttcc ctgatcccgg gctgggagcc ggcaattgtc agctctttta
      241 ccagtgcggt tatccggtct ttactgaatt cgggcatcga atagagcccc ttcaatccgc
      301 acaacagcaa caactccatg gcaggccgct gttttaaagc cggatcaacc gcaagcgaat
      361 ccagtatctc cgccagcggc gctttcccgt tgatcatatc gtaggtcttg ctataattga
      421 aatatttgcc attggtcaca aaatatttat caaaatagag atgaaagaaa tccatatatt
      481 cgagatggtt aaaaagaacc ggacgatcgg ccaggaattc ggtcccgatc ttttccctgc
      541 tttttatccg cataaataat tgcatggagg ccgatttgta gcggatataa ttatcgaaat
      601 actcgctgct tataccctgg aatctcagtt ccaccgcagt catgaaggtt tcataagcca
      661 ccctggaatt ctgctgtcga atgttatccg atatattcag taggaaatcg ttgtatatct
      721 cgttgaaatc ctgaatggaa ttatttaact gcatcgaatc gtcgcggatg atacgattat
      781 cgagggtacg acgattgtaa taagacatcg acagcggatc accggccaga tcgatctcca
      841 cttcgtacga ttgaccgggc tgaataaaca attcggcttg ttgaaattcg atatcgatcc
      901 agcaataggt cacagcagat atatcgacac ttaaggaaaa cctctcatcc tcaccggcca
      961 cagtgcttgc aagcgttttc ctgatatacg acaactggtc gttatatgtc atcaacctta
     1021 cggtataacc actcccgttt ttaagggtcc cggaaatggt ggtcaagtca ctaaagccat
     1081 taatggcaaa ggttaataga aatatacaga gcaagattga ttttttcata cccaatttct
     1141 atactaacga aagacaaggc ctgttatttg aattttaccg cctccggaac gaactgtgaa
     1201 ggatcgccgc cgttcctgag aatgtctctg actatggaag agttcagggc cgtatgttcg
     1261 ggcatggtca gcaggaaaac agtttcaacg tcggcattca ggtttttatt gatctggccg
     1321 atgctgcgtt cgaattcaaa atcggcggag gtacgcaatc cgcgcaggag aaactttgct
     1381 ttactggcga tgcaaaaatc gacagtaagg cctttataag acttaacctc gatggccggt
     1441 tcattcacaa agacctgttt tatccaatcc attcgttgtt caagggagaa gaagcttttc
     1501 ttctccgcat tctctcctat ggccacgatg attttatcga acagtggcat agcccgcctg
     1561 ataatggatt catgaccttt ggtgatcggg tcgaaagaac cggggaatac ggcaattctt
     1621 tccatacctt gatttttaaa caaagatata ttaatttagg gtgatgaata ctaaaccgca
     1681 agccggtgaa gttatgaaca aattaacccg ttaatgattt actttttact tttgccttat
     1741 gatttataac gaagaaattg ccagaacgac cgccaagtca cttctgcaga tcaaggctgt
     1801 caaacttaac cctacagagc cctttacatg ggcttccggg atcaaatcac cgatttactg
     1861 cgataaccgt ataaccctgt cgtttcatgc tatccgtact tacatccgcc agcagttcgt
     1921 tcatataatc cgtgaagaat tcggcgtggt ggagtatatc ggtgcagtgg ctacaggcgg
     1981 gatagcccag ggcgccctgg tggcccagga attcggcatt ccgctggtgt atgtcaggtc
     2041 ggagaagaaa tcgcacggaa tggagaacaa gatcgagggg aagatcgagc cggggaaatc
     2101 gatcgtggtc atcgaggacc ttgtatcgac cggcagcagc agcctgactg ccgtgcaggc
     2161 gctcaccgag gccggattca tcgtcaaagg gatggtcgcc attttcagct ataacctcga
     2221 accggccagg aagaactttg ccgacgccaa atgcaggctt gtgaccctgt cggattataa
     2281 cacccttatc aacgaagccc tcgacagcaa ttacatcacc gacgacgaca tgcagtcgct
     2341 ggtgaaatgg cgggaaaatc ctgaaaaatg gggaaagtaa cctttcaaag ccagcaggcc
     2401 accctcccgg taacggccgg cgaggtttac ctcttttttt atgacctcaa caacctgcgc
     2461 aagcttatgc ccgaacagat catcaactgg cagtcggata cggataaatg ttcgttcgat
     2521 atcaaaggga tggcgcatct tgacttgcaa cgcggggaga cagagcctga ccggctggtg
     2581 aagatcattt ccgccggcga cagtcctatc ggcctggaga tcaggggcat ctccgaagat
     2641 gccggcgaaa acagggctgt agcccggata gagatcgcgg ccgaactcag cccgatgctg
     2701 caaatgatgg tcagttcacc ccttcagaac ctggtgaaga tgatggcgga aaggctggag
     2761 gcggagatgc ggggttccta gttccaggtt ccaggttcca ggttccaggt tccaggttcc
     2821 aggttccaaa tttccaaatt ttcaaatttc caaattttca aatttcgaat ttcacctctt
     2881 tcaggtcgca ttcgaggatt ttactgcctg gaatttccag gatgagctgc ccgtaacgat
     2941 ttacgcctgt tatccgggct tcgacgggct tttctttaac aatgaacctt gccatctcgt
     3001 tgaatctgta caaaaccttc aggtaatcgg catccacaca tgttttatcg ccctttttaa
     3061 ggcattcgta ccgtgcttcg atcttcttca ggaccaattc caggaccccg ggcaggtcga
     3121 agattttacc cgacacctgg ttgagcgaga caggattggc tgcatcgtcg gtaaaccggg
     3181 actggttgac attcaacccg atcccaagga tggaaaactc aaaccgagag ccctttaccc
     3241 cgttctggat cagcgttccg gccactttcc ggtcgccgat atagagatcg ttcggccatt
     3301 tgacccggat atcggtgcgg cccggcaaaa gttcattcac ggcatctgcc agtccaagtg
     3361 aaatgacctt attcaggtag aattgtgctt ccggggccag gaaaaccggc ttcaggagca
     3421 ggctgaaggt gagattcttt gattgttcgc tttcccagcg ggcgccatcg gtacccctgc
     3481 cggctgtctg gaaatcggtg atcaccaccg tgccttccga tacattctcc ctgttgatcc
     3541 aatccatcag gtagcgattc gtcgaatcga cctcgtgaag cctgatgatc tcatttccga
     3601 ttgtacccat cattaaacaa ttttcacaaa attacgttaa taagccggaa gatatgattc
     3661 atttttcaaa taacctaaac gataagtgga tcaaaccgat agaatgcaaa aaagcttaat
     3721 tttgccgaca aataccagac catgactccg gaactatacg aatcagaaca gttgacagaa
     3781 ctcattatac aggggatgga ggacacgaaa gcgaaaaaga tcgtgatcct cgatttacag
     3841 gacatcgaca gtgccgtgac caattatttt gtgatctgtc acggcaattc caaaccgcaa
     3901 ctggaagcga ttgccgattc ggttatcgcc aaaaccgtca aagggctgaa ggcgaaaccg
     3961 tggcacaagg aaggttttga aaatgcggaa tggattttgc tggattacgt cgacgttgtc
     4021 gtacatatct tccgggaaga ccagcgtgag ttctatcaac tggagaaact gtgggcggac
     4081 gcaccgatta caaggcacga ttataaagaa taattcaaat attaagacag aatggcagag
     4141 gagaatttaa acaagcagga taaaggggga gcacaacaac ctaaaggaaa caacaaatcg
     4201 aaatatttcg gttactggtg gatcgggctg gtatttatac tgttgctgac ctacctggtg
     4261 aactgggata cgggcacagc caaaaaggcc acctggggtg aagtcaagga gatgctggcc
     4321 accggcgaca ttgagcgtat tgtgctggtc aacaaggagg cggccgaatt ttatctcaaa
     4381 aacgaaaagc tggatgatcc aaaatacaag gagatcaaag agaccgttgc tgccaaaggg
     4441 ccgcactata cctataatat cacctcccct gaaagttttg aaaggatggt tgccgaggcg
     4501 caggaaggca ttgccgatcc gatctacatc gagaacgacg tcaggcgcaa ctggacaggc
     4561 gaagtgctga gctggatcgt gccaattgct attcttatca tcatctggct ggtgatcatg
     4621 cggatgatga gccgcaatgc cggcgggccg ggaggacaga tcttcaacat cggcaaatcg
     4681 aaagcgcagt tgttcgacaa gaccaccagc gtctcgatca atttcaacga tgtggcgggt
     4741 ctggaggagg ccaagctgga ggtgaaagag gtggtggact tcctcaagaa cccctcaaaa
     4801 tacaccgaac tgggcggcaa aatccccaaa ggcgtattgc tggtgggccc tccggggacg
     4861 ggcaagacat tgctggccaa agccgtagcc ggcgaagcac aggtcccctt cttctccctt
     4921 tcgggttccg atttcgtgga gatgtttgtc ggggttggcg catcgcgtgt ccgcgacctg
     4981 ttcaagcagg ccaaggagaa agcgccgtgt atcgttttta tcgatgaaat tgacgccatc
     5041 gggcgcgccc ggggcaagaa ccagatcacc ggcgccaacg acgagcggga gagcacgctg
     5101 aaccagttgc tcaccgagat ggacggcttc agcaccaaca gcggtgtgat catcatggcg
     5161 gcgaccaacc gggccgacat ccttgaccgg gcgttgctgc gtgccggccg tttcgaccgc
     5221 cagatcaacg tggagctgcc tgacctggac gagcgccgcg ctatttttgc ggttcatacc
     5281 aagaacctga aattcgacaa gaccagtgtt gacctggaat ggctggcaag gcaaacgccc
     5341 gggttttcgg gagccgacat agccaacgtg gccaacgaag cagcgctgat cgctgcccgt
     5401 aacaacaaaa agatcatcga gaagcaggat tttatcgacg ccgtcgaccg gatcatcggc
     5461 gggctggagc ggagaagcaa gatcatttcg caggaagaga agcgggtgat tgcgttccac
     5521 gagtcaggcc atgctgccgt aagctggctc ctcccccatg cccatccgct ggtgaaggtg
     5581 accattgtgc cgcgcgggaa agcgctcgga gccgcctggt acctgccgga agagcggcag
     5641 atcaccacct tcgaccagat gttcgacgag atgaccgcca cgctgggcgg acgggccgct
     5701 gaagagatca ttttcggcaa agtgtcgacc ggcgccctga acgacctgga gcgggtgacc
     5761 aagcaggcgt acaacatggt tgttttcttc ggcttgaacg agaagctcgg caacatcagt
     5821 tactacgatt caacggggca gcaggaatac atgttcaaca agccctacag cgagaaaacg
     5881 gccgagatca tcgaccagga gatcggcaag cttattgacc aggcgtacaa acgtgcggtt
     5941 gaaattctca gaacccataa agacaaactg gaggccctgg ccagtatttt actggaaaaa
     6001 gaggtcattt tcaccgagga tctcgagacc attttcggca agaagatcaa gcatgtcggc
     6061 cacgaagtgg tgctggccga tcccaatggc aacgggcatg ttgaggcgcc tgctgtggtt
     6121 gaagaggcga aaccgaagaa aagggggcgg aagccaggat cagttgggca gttggacagt
     6181 tgacagtatg cagtaggcag taggcagtaa ggtgtaatga tataggtaac ctctcccgcc
     6241 agggagaaca gtatggcagg cagggacgtc acacagaaac agggtttcca accctgaaat
     6301 ttgggatctt tgaataaatt gaaaaacgtt gatcttcgtg gatatagttt ttgga
//
LOCUS       DAOQXS010000100         6330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12667_length_6330_cov_5.475219, whole genome shotgun sequence.
ACCESSION   DAOQXS010000100 DAOQXS010000000
VERSION     DAOQXS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12667_length_6330_cov_5.475219"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2422
                     /locus_tag="PKM34_04135"
     CDS             <1..2422
                     /locus_tag="PKM34_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82808.1"
                     /translation="DLIVPEGTVLNWRFFTRDTRNILLRWGKETSELSNPSSNAFSVS
                     KRAHASVTYSVASVNQYLRNSDSLTYTVSVIPDAYPSVSVEQVRDSIYDKRIYFRGLI
                     KDDYGFSKLTFNYRLDPEGDADANDYPEIIAELPVSKLHNQQQFFHFFELDSINIQPG
                     EQIVYHFEVWDNDAVNGSKVTRSQPMIFRAPTLDEIEEKTEESNQEIMEELENSIRNA
                     KDLQKKVDELNRKLVDKEEIGWQEKQQIQELLDQQKELQETIEKIQKENELKSIQEQE
                     YKEINEELVRKQQRLEEFFEQIMTDEMKKMFEELQKLLEEVDKDKVNEMLEEMKKDNK
                     DLEKQLDRNLELFKQLEFEKKLQESIDKLKELAGKQDEQAKKSDDRKADAEKLKEEQD
                     KLNEEFRQLRDDLDRLEQKNKELEEPHQLENTEQMEQEIQDNMEEGSDQLQQGKPSKA
                     SPKQKSAAEGMKSMASKMEQMQSEMYEEELGENIETLREILENLVQLSFDQEELIGRT
                     LEISVIDPQYPSVIEAQNRIRDDLVMVEDSLWALSKRQQMIEPFITREIQDINHHVEA
                     ALEDLTERRKSSAAEDQQFVMTSVNDLALLLSETLQQMMNAMQMQCSGQCKNGSPKPG
                     KGSMSMKSMREMQQKLNQQIQQMKQEGSTPAKTKESRNSAQSEGFARMAAQQEAIRRM
                     MGEYQDQMKEQGMGNSKELQEIMEQMERTETELVNKIITQQTIERLKEIETRLLKHEK
                     AELKREQEEKRESREGKDFNKGNPEQFLEYNKIQDKEIELLRTVPPDLRPYYKEKVNQ
                     YFYYFEMR"
     gene            2426..2908
                     /locus_tag="PKM34_04140"
     CDS             2426..2908
                     /locus_tag="PKM34_04140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ82809.1"
                     /translation="MNRPKEFTIGSTLEEMYRIEQFVDEISDEYLLYGSYFGNILMAL
                     TEAVRNAVVHGNGNDSRKRVYIRQEKVKAGLMLTVSDEGQGFDFKTALESAANLKAPE
                     TSGNGLFLMQKLSDEINFRNEGRTVEMLFRINGIDESIFNRRAECMNDFFKVYQKVNS
                     "
     gene            2920..3351
                     /gene="ybeY"
                     /locus_tag="PKM34_04145"
     CDS             2920..3351
                     /gene="ybeY"
                     /locus_tag="PKM34_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNQ82810.1"
                     /translation="MAKESNIFFFLEEISYQLKNKRKLRHWITQAVENEGHKINNLNY
                     ILTSDNILFQLNKEYLQHNTLTDIITFDMSESEGELAGDIYISVDRARENAKEYHVSL
                     TNEISRLMIHGVLHLMGYKDKTNEERVVMRAREEFYLSLLI"
     gene            3407..5278
                     /gene="mnmG"
                     /locus_tag="PKM34_04150"
     CDS             3407..5278
                     /gene="mnmG"
                     /locus_tag="PKM34_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNQ82811.1"
                     /translation="MFKEYDIIVVGAGHAGCEAAAAAANLGSSVLLITMNMTNMAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVTDKTMIQFRMLNMSKGPAMWSPRAQSDRMLF
                     SIEWRKMLENTPNLDFWQDMVTGVLVKNGVATGVSTAMGHEIYSRSVILTNGTFLNGI
                     IHIGLKQFGGGRIGEKAAKGLTECLVELGFESDRMKTGTPVRVDGRSIDFSKLEEQKG
                     DNDPRRFSFTKTPPLTRQRSCYIAYTSREVHETLKTGFHESPMFTGRIEGIGPRYCPS
                     IEDKINRFSGKESHQLFVEPEGWETEEYYINGFSSSLPDHVQFNALRKITGFENARIF
                     RPGYAIEYDYFPPVQLNYTLETKKVPGLYFAGQINGTTGYEEAAAQGIVAGINAHLKV
                     HRSEEFILKRSDAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQSNADQRLTPLAH
                     SLGLASAERYQTVMSKSEKIHEVVRFLQKTSTDPDQVNPYLVNAQTAPIDQKVKLATL
                     LLRPQISLDGLAGQIPFLQEYLGRVAPDRVEILEEAEIMIKYQGYIDKEGELAQKLER
                     LDSVTLKEDFDYHRLTALSFEAREKLSRIRPRSLGQASRIAGVSPSDISILIVHLGR"
     gene            5818..>6330
                     /locus_tag="PKM34_04155"
     CDS             5818..>6330
                     /locus_tag="PKM34_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82812.1"
                     /translation="MARPIIHHGNGWYRVMDKTAGSLKAQVLILIRTDYPYQNQYLIN
                     QFHEDFQLDPDAGLDTLKRDLNIFSEDGDFLFSLIPPDTWQTNEGIGLAVFGLAIIAF
                     ICMVLVLFYLYHLFDFFRRYPVIMFIAFCADAVVLRLAMIWFEIPGFLYQTALFSPLY
                     YATSFFSPSLG"
BASE COUNT         1923 a         1362 c         1599 g         1446 t
ORIGIN      
        1 cgatctgatt gtccccgagg ggaccgtact caactggcgg tttttcacac gggatacgcg
       61 gaatatactg ctcagatggg gtaaagaaac ttcggaactg agcaaccctt cttccaatgc
      121 attttccgtt tcgaaaaggg ctcatgcatc ggttacctat tcagtagcct ctgtgaacca
      181 gtacctccgg aattccgact cgctgacata tacggttagc gttatccctg atgcctaccc
      241 ttcagtttct gttgaacagg ttagggattc gatttatgat aaacgaatat atttcagggg
      301 attaattaaa gacgattacg gtttctcaaa actcacgttc aattaccggc ttgaccccga
      361 aggagatgcc gatgccaacg actacccgga gatcatcgcc gagcttccgg tcagtaaact
      421 gcacaaccag cagcagttct tccatttctt tgaactcgat tccattaata ttcagccggg
      481 tgagcaaatc gtttaccatt tcgaggtctg ggataatgat gctgttaacg gcagcaaggt
      541 aacccgctct cagccaatga ttttcagggc ccctactctg gatgagatag aagaaaaaac
      601 agaagagagc aaccaggaga ttatggaaga actggagaac agtatccgga atgcgaaaga
      661 ccttcaaaag aaagtggatg aacttaaccg gaaacttgtc gataaagagg agatcggatg
      721 gcaggaaaaa cagcagatcc aggaattgct cgaccagcag aaagagctcc aggaaacaat
      781 tgaaaagatt cagaaggaaa atgagctgaa atccatccag gagcaggaat acaaagagat
      841 caacgaggag ttggtaagaa agcagcaacg ccttgaagaa tttttcgaac agatcatgac
      901 ggatgagatg aagaagatgt tcgaagaact tcagaagctt ctggaggaag ttgataagga
      961 taaggttaat gagatgcttg aagagatgaa gaaagacaat aaggatctgg aaaagcaact
     1021 cgaccggaac ctggaattgt tcaagcaact ggagtttgag aagaagctgc aggaatcaat
     1081 agataaatta aaggaattag ccggaaaaca ggatgagcag gcaaagaaga gcgacgaccg
     1141 taaagccgat gcggaaaagc ttaaagaaga gcaggacaaa ttaaacgaag agttccggca
     1201 gcttcgcgat gatctagaca gactcgaaca gaagaataaa gaacttgagg aaccgcacca
     1261 gcttgaaaac accgaacaga tggagcagga gatccaggat aatatggaag agggatccga
     1321 tcagctgcaa cagggaaaac cgtcaaaagc atctcccaag cagaaaagcg cagcggaagg
     1381 gatgaaatca atggcatcga aaatggaaca gatgcagagc gaaatgtatg aagaggagct
     1441 tggtgaaaat atagaaacgc tgcgggagat cctggagaac ctggtccagc tttcgtttga
     1501 ccaggaggag ctgatcggca gaacactcga aatttcggtg attgacccgc agtatccttc
     1561 ggtcatcgaa gcccagaacc ggatcaggga cgacctggtt atggtggagg attcgctgtg
     1621 ggctttgagc aaaaggcagc aaatgatcga gcctttcatc acacgggaaa ttcaggacat
     1681 aaaccatcat gtcgaagcgg cgctggaaga ccttaccgag aggagaaaat cttctgcggc
     1741 agaagaccag cagtttgtga tgacatcggt aaatgattta gctttgttgt tgtcggaaac
     1801 acttcagcaa atgatgaatg ctatgcagat gcagtgttca ggtcagtgca agaacggcag
     1861 cccgaagccc ggaaaaggca gcatgtcgat gaaaagcatg cgtgaaatgc agcaaaagct
     1921 gaatcagcag atacagcaaa tgaagcagga aggttccacg ccggcgaaga cgaaagagag
     1981 ccggaattca gcccagagcg aaggttttgc caggatggcg gcccagcagg aagcgatccg
     2041 gcggatgatg ggggaatacc aggaccagat gaaagagcag ggaatgggca acagtaaaga
     2101 gcttcaggag atcatggagc aaatggaacg aaccgagacg gaacttgtta acaaaatcat
     2161 aacccagcaa acgattgagc gtttaaagga gatagaaacc aggcttctca aacacgaaaa
     2221 agcggagctt aaaagggagc aggaagaaaa aagagaatca cgcgaaggaa aagatttcaa
     2281 caaaggaaac ccggagcaat ttttagagta taataagata caggataaag aaattgaatt
     2341 gttgaggacg gtaccacccg acctgcgtcc ttattataaa gagaaagtga atcagtattt
     2401 ctattatttt gaaatgcggt aggagatgaa cagaccgaaa gaatttacaa taggatccac
     2461 tctggaggag atgtaccgga tcgaacaatt tgtggacgaa atcagtgatg agtacctgct
     2521 ttacggaagt tacttcggaa atattctgat ggcgctgaca gaggccgtca gaaatgctgt
     2581 cgttcacgga aacggaaatg attcccgaaa aagggtatat atccggcagg aaaaggttaa
     2641 agccggattg atgctgaccg tttcagacga aggtcagggc tttgatttta agacggccct
     2701 ggaatcagcc gcgaacctga aggctcctga aacgtcagga aatggtttgt tcctgatgca
     2761 aaagctttcc gatgagataa acttccggaa tgagggcagg actgtggaaa tgcttttcag
     2821 gatcaatggg atcgatgaaa gcatattcaa ccggcgggca gaatgcatga atgatttctt
     2881 caaagtttac cagaaagtga acagttgacc taataataaa tggcgaaaga atcaaacata
     2941 ttcttttttc tcgaggagat cagttatcaa ctgaagaaca agagaaagct tcgccactgg
     3001 attacgcaag cggttgaaaa tgaaggacat aagattaaca atctgaatta tatcctgaca
     3061 agtgataata tacttttcca gttaaataag gaatatctcc agcacaatac ccttaccgat
     3121 atcattactt ttgatatgtc ggaatcggaa ggtgaacttg ccggagatat ctatatcagt
     3181 gttgacaggg ccagggaaaa cgcaaaagag tatcatgttt cgcttaccaa tgagataagc
     3241 cgtcttatga tccacggagt gcttcacctg atgggctata aggataagac gaacgaggag
     3301 cgggtggtta tgcgggcccg ggaggaattt tacctatctt tgctcatata aatgatttgt
     3361 tccacgtgga acatcaatga tctaaaattc ccattcattt acagggatgt ttaaagaata
     3421 cgatataatc gtcgtgggcg caggccatgc aggttgcgaa gccgccgccg ctgccgcaaa
     3481 cctgggttca tccgtattgt tgattaccat gaatatgacc aatatggccc agatgtcgtg
     3541 caacccggcg atgggaggca tagcaaaagg ccagattgtc cgggaaatcg atgcccttgg
     3601 cggatattcg ggtattgtca ccgacaagac gatgatccag ttcaggatgc tgaatatgtc
     3661 gaaagggccg gccatgtgga gcccccgggc ccaaagcgac cggatgttgt tttcgataga
     3721 gtggagaaaa atgctggaaa acacccccaa tctggatttc tggcaggata tggttaccgg
     3781 tgttctggta aagaatggcg tggccaccgg cgtttctaca gcaatggggc atgaaattta
     3841 ttccagatca gtgatcctga caaacggcac ctttttaaac gggatcatcc acatcggatt
     3901 aaaacagttc ggaggaggga ggattgggga aaaagcagcg aagggcttga ccgaatgcct
     3961 ggtggaactg ggttttgaat cggataggat gaaaacaggt accccggtga gagttgatgg
     4021 aagatctatt gatttttcga aactggaaga acagaaaggc gataacgatc cacgaaggtt
     4081 ctctttcaca aaaactccac ccctgacccg gcagagaagt tgctatatcg cttataccag
     4141 ccgtgaggtg catgaaaccc tgaaaacggg tttccatgaa tcaccgatgt ttaccggacg
     4201 gatcgaagga attggcccaa gatactgtcc gtcgattgaa gataagatta acaggttctc
     4261 cggaaaagag agccaccagc tcttcgtgga accggaagga tgggaaacag aagagtatta
     4321 tatcaacggc ttttcatcct ctttgcccga tcatgtccag ttcaacgccc tcaggaaaat
     4381 aacgggcttc gaaaatgcac gtattttccg ccccggctat gcgattgagt acgactattt
     4441 tcccccggtg caattgaatt ataccctgga gactaaaaag gtgcccgggc tgtattttgc
     4501 cggacagatc aacggcacga cagggtatga ggaagctgcg gcccagggga tcgtggccgg
     4561 gatcaatgca cacctgaagg ttcacagaag cgaagaattt atcctgaaaa ggtcggatgc
     4621 ctacatcggc gtgctgatcg acgacctgat caccaaaggg gtcgacgagc cctaccggat
     4681 gttcaccagc cgggccgaat acaggatcct tttgcgccag agcaatgccg atcaacggct
     4741 gacaccgctg gctcattccc tggggctggc ttcagccgag cgttatcaaa ccgtgatgtc
     4801 gaaaagtgaa aagatccacg aggtggtcag gtttttgcag aaaaccagca ccgatcccga
     4861 tcaggtcaac ccttacctgg taaatgccca gaccgctccg atcgatcaga aggtgaaact
     4921 ggccaccctt ctgctacggc cgcaaatatc ccttgacgga ctggccggcc agatcccgtt
     4981 cctgcaggag tacctcggtc gtgtcgcccc tgatcgagtt gagattctcg aagaagcaga
     5041 gatcatgata aaataccagg gttatattga caaggagggt gaactggctc agaaactgga
     5101 acgactcgat tccgttacac ttaaagaaga cttcgattac catcgcctca ctgccctctc
     5161 ttttgaagca cgggaaaaat tgtcgcggat ccgtccacgg tcacttggac aggcttcgag
     5221 aattgcaggt gtctcccctt ccgatatctc catcctgatc gttcatcttg gccgctgaac
     5281 gttccacgtg gaacaatatt tgaagtattc tatccaatcg ggatatttct taattttaag
     5341 ccctgtgatc cattttagtg cccggataag gattagcccg gctttttgaa aataattcgt
     5401 tatagggcct gaaatcaagc ggcatttaaa taaatgaaat tcagctaata agatcattta
     5461 atggcaagaa tgtcaaaata cggtcaaaat gctctccttt tttttagcgt gaccatcctg
     5521 atggttattg ccatgttgat cattcactat tccgcaggta aagcaaaaaa ccagttcatt
     5581 tctaaacaat ctaatgttga gcatgtcctc cggcataaag aatctcaggc atcggaaatt
     5641 ctggatcaga tccttctacc tgaatttgca gaaaacccgt ttctaaagtt ttggaaaaac
     5701 gaaaaaagtt ttaattggca ggaaaacgga ctgacagcac tgctgtatag aaacgattcc
     5761 cttatatttt ggtccgacaa ttcaattccg gccccgggac gcattgaaag aaatggattg
     5821 gccagaccga tcatacatca cggtaacgga tggtacaggg tcatggataa aactgccggt
     5881 tcgttgaaag cacaggtgct gatccttatc cggaccgatt acccttacca aaaccagtac
     5941 ctgatcaacc agtttcatga agattttcaa cttgatcccg atgccggact ggacaccctg
     6001 aaaagagacc tgaacatctt ttcggaggat ggagattttc tcttttccct cattcccccg
     6061 gatacgtggc aaaccaatga agggataggc ctggccgttt tcggcctggc tattatcgcc
     6121 tttatttgca tggtactggt tttattttac ctgtaccacc tctttgattt cttccgacgc
     6181 tatccggtga taatgtttat cgccttttgt gcggatgctg tcgttctccg gctggcgatg
     6241 atctggttcg aaatcccggg attcctttat cagacagccc tcttcagccc actatactac
     6301 gccacctcct tcttctctcc ttcactgggc
//
LOCUS       DAOQXS010000101         6328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12679_length_6328_cov_4.093735, whole genome shotgun sequence.
ACCESSION   DAOQXS010000101 DAOQXS010000000
VERSION     DAOQXS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_6328_cov_4.093735"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..2106
                     /locus_tag="PKM34_04160"
     CDS             415..2106
                     /locus_tag="PKM34_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82813.1"
                     /translation="MKSVLRLLIFLTVLQSNSPLIHAQGQFSKNRETYYEATKQKILG
                     NLDKAIELFKKCIELDSKDAAAMYELASIYTDLGKPAEALPFAEKAVAADPSNSWYKI
                     LLVRILQAQGKYSDAAKTIDQLLVSDPENIDYYQDQALNYLYDGDYKNAVKSYDMLEQ
                     KIGINEDLSVQKEKIYLMIGKTDKAIEEIRKLGQAFPDEPRYMEMLAELYMNTGMYDQ
                     ALELYNRILEADPDNPYINISLSDYYRKTGNRDKAYEYLKAGFANPALDIDSKVSILL
                     AYYSVTDIYSDYKEEAFELAEILIRAHPNDPKAHSIYADFLVQDKRYAEARDTFRKVI
                     SLDSTKYLIWEQLLFVESELNDNQAMAAESKRALDLFPDQPMLYMFAGAAHFMLKDYE
                     AAVKYFRAGVNFVVGNDRMLEQFYSYLGDSYFQLKDHEKSDEAYEKVLKINPANTLVL
                     NNYAYYLSLRGKDLEKAEQMAERAVEMEPDNSSYLDTYGWVLFKLGRYEEAKPWIERA
                     MEKGENSAVVLEHYGDVLWKLNDKKEAVKYWEKALDAGTGSEFLEMKVRDKEYHD"
     gene            2099..2917
                     /locus_tag="PKM34_04165"
     CDS             2099..2917
                     /locus_tag="PKM34_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNQ82814.1"
                     /translation="MIKIFKRIGMMGLVILALSSCRSTRPLIKEPLKEQGPEYLYSRL
                     KQSEFRFDRLSMKFTASYSDKKSSADLNGQIRIRKDSLIWITASPAMGLEMFRMVITT
                     DSVKYLNRMNKDYFAGDYALVSRFLQIDVDFDMLQSILLGNDFQFFETNNFRASIDNL
                     NYKLSTTGRRKIRKEAESAKNDPVILLQNIWLDPSSYKIKKIDLKEYMKDNRKLTATY
                     GDFQALNDQIYPSDIRFEIQAEEKSKVRVKYDKVATGEQMTFPFSIPPNYQRIR"
     gene            2964..4184
                     /locus_tag="PKM34_04170"
     CDS             2964..4184
                     /locus_tag="PKM34_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNQ82815.1"
                     /translation="MNRFTFFFILLIMPFTVAFAQKDRTKLEQDKKKIEEEIEYTNKL
                     LDQTRQTRQNSLNEVVILNKKISQREQLINTISSEIGLVDGQMALTLDTIKLLENDLK
                     SLKDEYAQMIYYAYKNQNLYDRMLFIFSAKDFNQAYQRLKYFEFYNQYRRQQALLIQV
                     KQKRLEIKMEELAAIRTQKNNLLTGEERQRQQLTVEREEKNKSVKALSKKEKELQKTL
                     KEKEAAAKKLQQAIQEIIAEEIRLANERASKYGAPLPKTGVYALTPEEKIISDNFQSN
                     KGKLPWPVEQGIVSGTFGEHAHPVLKNVTTRNNGIDLLTQAGTEARAIFSGVVTRVMS
                     VPNNNNVVIIRHGEFLSVYSNLDQVYVKIGEKVTTKQKIGTVFTNKEDSKTELHFEVW
                     QSKTLQNPVEWLAK"
     gene            complement(4198..6189)
                     /locus_tag="PKM34_04175"
     CDS             complement(4198..6189)
                     /locus_tag="PKM34_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ82816.1"
                     /translation="MKKITSFFIFLIIIPFLISNTPANKHEKPYAEGEIMIKLYSDLP
                     QNQQQMLQDVLGDFQGAGLGVIRRLSDRLNIFLLSYDPSVISDDRMLQEIKSHPFVEL
                     AQFNHFIELREFIPNDPSFSQQWNMNNTGQTGGQPDADIDGPEAWGISTSGVTATGDT
                     IIIAVVDDGFDLEHEDIRFWKNYQEIPGNGIDDDSNGYIDDYHGWNSWNNSGNLVEKD
                     HGTHVTGIAAAKGNNGLGVTGVNMNVKVMPVVGSATTESIVVGGYSYVYEMRWLYNKT
                     DGAKGAFIVSSNSSFGTDGDIEDFPIWSAMYDSMGMQGILNAAATVNANLNIDVVGDI
                     PTAFPNEALITVTNTTSDDELSPYAGYGLTTIDIGAPGTQVYSTRYNNSYGLKTGTSM
                     SSPHVAGAIAYLFSVADESFMESYHADPAGMALVLKQYILNGADPIPTLANKSVSGGR
                     LNIYNAAQLLLNPAISFDPMSLLQMMQPNEQESVILSFTNNSASSVSYEIALAGTFPW
                     IALTGATSGTLSGFGTGSIQVDFNTTGMPYDTLQTYIAFNYGNDGLFNVPVHLLIGPF
                     IGLDDLSGNPDRLSKSLAIWPNPARDVIRFKVAGVSEGSVYSIDMVSADGRIHHTLNS
                     GSNQNELILDARSFKPGMYLAILKENDQVIDSQKILISR"
BASE COUNT         1749 a         1491 c         1683 g         1405 t
ORIGIN      
        1 aataaaatat aacggaatgt ctgttgtaat attgggcatg ctgcgaaaag tcaattttcc
       61 ggaagatgcc acaaaagcac caatacacca aatggcacag aaaccgggtt tccaaccctc
      121 atatcaatta caggaacacg aatgaaacga attgaacgga tgatcacgga ttgtaaaatg
      181 gaagccgaat ggatacgtaa agttgccgat ccccgattca agagaaataa aacctaattt
      241 tgcaccctca agacaatcct gaaacaattg aacaattgaa caattgaaca attgaacagt
      301 tgaacaattg aacaattgaa cagttgaaca gttgaacaat tgaacaattg aacaattgaa
      361 caattgaaca gttgaacaat tgaacagttg aacaatgaat aatcactccg aactatgaaa
      421 agtgtattgc gtcttctgat ctttcttact gtcctacagt ctaacagtcc tttaatccat
      481 gcccaggggc agttctcgaa gaaccgggag acctattacg aagcgaccaa acagaaaata
      541 ctgggtaatt tggacaaggc cattgaactt ttcaaaaagt gcatcgagct ggattcgaaa
      601 gatgcagcgg ccatgtacga actggcatcg atctataccg acctgggaaa accggcagag
      661 gcattgccat ttgcggaaaa agccgtagca gccgacccgt caaattcctg gtataaaata
      721 ctgctggtac ggatcttaca ggcacaggga aaatattccg atgcagctaa aactatcgat
      781 cagttactgg tcagcgatcc ggagaatatc gactattacc aggaccaggc gctgaactac
      841 ctgtatgacg gcgattacaa gaatgcggtc aaatcgtacg acatgctgga acagaagatc
      901 ggtatcaatg aagatctttc ggtgcagaaa gagaagatct acctgatgat aggtaaaaca
      961 gacaaggcga tcgaagagat ccggaaactc ggccaggctt ttcccgatga gccccggtac
     1021 atggagatgc tggccgagct atacatgaac accgggatgt atgaccaggc gctcgagctc
     1081 tacaaccgga tccttgaagc cgacccggat aatccttaca tcaacatttc gctttccgac
     1141 tattaccgga aaacaggcaa ccgcgacaaa gcttacgaat acctgaaagc agggtttgct
     1201 aacccggccc tcgatatcga ttccaaagtg tcgatcctgc tggcttatta cagtgtcacg
     1261 gatatctaca gcgattacaa ggaggaggcg tttgagctgg ctgagatcct catccgggcc
     1321 catccgaacg atccgaaggc ccactcgatc tatgccgatt tcctggtgca ggacaagcgg
     1381 tatgcagaag cccgcgatac attccgcaaa gtgatcagcc tcgacagcac aaagtacctg
     1441 atctgggaac aattgctctt tgtggagtcg gagctgaacg ataaccaggc gatggctgcc
     1501 gagagcaagc gggcgctgga ccttttcccc gatcagccca tgttgtacat gtttgccggg
     1561 gcggcccatt ttatgcttaa agattatgag gcggcggtaa agtacttcag ggccggcgtt
     1621 aatttcgtag tgggcaacga ccggatgctg gagcaattct attcctacct cggcgattcc
     1681 tatttccagc tgaaagatca cgagaaatcg gatgaggctt atgaaaaggt gctgaagatc
     1741 aacccggcca atacactggt gctgaacaac tatgcctact acctgtcgtt acggggaaaa
     1801 gacctggaga aggcggaaca gatggctgaa agggccgtgg agatggagcc cgacaacagt
     1861 tcctacctcg acacctacgg atgggtgctg tttaagctgg gccggtatga agaagccaaa
     1921 ccatggattg aaagagccat ggaaaaaggg gaaaacagtg cagtcgtcct ggaacattac
     1981 ggcgatgtac tgtggaaact gaatgataaa aaagaagcgg tgaaatactg ggagaaggcc
     2041 ctggatgccg ggaccgggtc ggagttcctt gaaatgaagg tccgggataa agaataccat
     2101 gattaagatt tttaagagaa tagggatgat gggcctggtg atcctggcgc tgtcatcgtg
     2161 ccgttcgaca cggccactga tcaaagagcc gctgaaggaa caggggccgg agtaccttta
     2221 cagccggctg aaacagagcg aattccggtt cgaccggctg tcgatgaagt ttacagcctc
     2281 gtattccgac aaaaagagct ctgccgacct caacgggcag atcaggatca ggaaagacag
     2341 cctgatctgg atcacggcat caccggccat ggggctggag atgttccgta tggtgataac
     2401 caccgattcg gtgaagtacc tcaaccggat gaacaaagac tattttgccg gcgattacgc
     2461 actggtcagc cggttcctgc aaatcgacgt tgatttcgac atgctgcagt cgatcctgct
     2521 gggaaacgat ttccagtttt ttgagacgaa taatttccgg gcttccatcg acaacctgaa
     2581 ttataaactt tccactaccg gaaggcgtaa gatcaggaaa gaagcggaat ccgccaaaaa
     2641 tgacccggtc attcttttgc agaacatctg gctcgatccg tcatcttaca aaataaaaaa
     2701 aatagacctg aaggaatata tgaaggataa ccggaagctt acagccacgt acggtgattt
     2761 tcaggccctt aacgatcaaa tctaccccag tgatatccgt ttcgagatcc aggccgaaga
     2821 gaaatcaaag gtcagggtga aatatgataa agtagccacg ggagagcaaa tgaccttccc
     2881 cttctcaatt cccccgaatt atcaaaggat caggtgattt attcctattt ttgtttcatc
     2941 gataaaattc tttacggaac cggatgaaca gattcacctt tttctttatc cttttgataa
     3001 tgccttttac ggttgctttc gcccagaagg accgtacaaa gctggagcag gacaagaaaa
     3061 agatcgaaga ggagatcgaa tacaccaaca agctgctgga tcagacacgg caaacccggc
     3121 agaactccct caatgaggtg gttatcctta ataaaaagat cagtcagcgc gaacagctta
     3181 tcaacacgat cagcagcgag atcgggctgg tcgacgggca gatggccctg accctcgata
     3241 caattaaatt gctggaaaac gacctgaaga gcctcaaaga cgaatatgcc cagatgatct
     3301 actatgcata taagaaccag aatctttatg accggatgct cttcatcttt tctgcaaaag
     3361 acttcaacca ggcctatcag cggctgaaat atttcgaatt ttacaaccag taccggcggc
     3421 agcaggcgtt gcttatccag gtaaaacaaa agcggctgga gataaaaatg gaggagctgg
     3481 ccgctatcag aacgcagaag aacaatctgc tgaccgggga agagcgccag cggcagcagc
     3541 ttaccgtgga acgggaggag aagaacaaat cggtgaaagc cctttcgaag aaggagaagg
     3601 aactgcagaa gacgctgaaa gagaaagagg cggcggcaaa gaagctccag caggccatcc
     3661 aggagatcat cgccgaggag atcaggctgg ccaatgaaag ggcaagtaaa tacggcgccc
     3721 cgctcccgaa aacgggcgtt tatgcgctga cgccggaaga aaagatcatt tccgataatt
     3781 tccagagcaa taaaggcaag ctgccctggc cggtggaaca ggggatcgta tcgggcactt
     3841 tcggagagca tgcccacccg gtacttaaga atgtgacgac gcgcaacaac ggcatcgacc
     3901 tgctcaccca ggccggcacc gaagcccggg ccattttcag cggcgtcgtt acccgcgtga
     3961 tgagcgtccc taacaacaac aacgtggtca tcatccgcca cggcgaattc ctttcggtct
     4021 attccaacct cgaccaggtt tatgtgaaga tcggggagaa ggtgacgacg aagcagaaga
     4081 tcggcacggt tttcaccaac aaggaagatt caaaaacgga acttcatttc gaagtgtggc
     4141 agtcgaaaac actccagaac ccggtggaat ggctggcaaa atgagttaac cccacattta
     4201 ccttgatatc aagatcttct gtgaatcaat cacctggtcg ttttctttaa gaatggccag
     4261 gtacataccc ggtttaaatg aacgggcatc caggatcagt tcattctgat tcgacccgga
     4321 attcaaagta tgatggatcc ttccgtcggc gctgaccata tcaatggaat aaacactccc
     4381 ctcacttaca cccgcgacct tgaacctgat aacgtccctg gccggattgg gccagatggc
     4441 caggcttttc gataacctgt ccggatttcc cgacagatca tcaagaccga taaaggggcc
     4501 gatcaggaga tggacgggga cgttaaacag gccgtcgttg ccgtaattaa aggcgatata
     4561 ggtctggagt gtatcgtaag gcattccggt ggtgttgaag tcgacctgga tgctgccggt
     4621 accgaatcca ctgagggtgc cggaggtggc gccggtgaga gcgatccacg ggaaggtgcc
     4681 tgccagggcg atttcataac taacactgga cgccgagtta ttggtgaaag agaggatcac
     4741 gctctcctgc tcgttgggct gcatcatctg gaggaggctc atcgggtcga aggagatggc
     4801 gggattgagc aggagctgtg cggcgttgta aatgttgagg cgtccgccgg agaccgattt
     4861 attggccagg gtaggaatcg ggtctgcgcc attgagaata tattgtttca gaacgagggc
     4921 catgccggcc ggatcggcgt gatacgattc cataaaggat tcatcagcaa ctgaaaatag
     4981 gtaagcgatg gcgccggcca cgtgcgggct cgacatcgag gtgccggtct tgaggccgta
     5041 tgaattgtta taccgggtgc tgtaaacctg ggtgccgggg gcgccgatgt cgatggtggt
     5101 caggccatag ccggcatagg ggctcagctc gtcgtcactg gtggtattgg tcaccgtaat
     5161 gagcgcttcg ttggggaagg ccgtaggaat atcgcccacc acatcgatat tcaggttggc
     5221 attgactgta gcggccgcat tgagaatgcc ctgcatgccc atggagtcgt acatggcgct
     5281 ccagatcggg aaatcttcga tgtcgccatc ggtgccgaaa gaggaattgg atgaaacgat
     5341 gaaggcgccc tttgcgccgt cggttttatt gtagagccac cgcatctcgt aaacatagga
     5401 gtagcctccc accacgatcg attcggttgt cgccgagccc accaccggca tcacttttac
     5461 gttcatgttc acgccggtca cgccgaggcc gttattgccc tttgctgcag caatgccggt
     5521 cacatgcgtt ccgtggtctt tctccaccag gttgccggag ttgttccacg aattccagcc
     5581 gtggtaatca tcgatatagc cgttggaatc atcgtcgatc ccgttgcctg ggatttcctg
     5641 gtagttcttc cagaagcgga tatcttcatg ttccaggtca aatccgtcgt cgaccacggc
     5701 aatgatgatc gtatcgccgg tggcggtaac acctgaagtg cttatgcccc aggcttccgg
     5761 cccgtcgata tcggcatcgg gctggccgcc ggtctgccct gtgttgttca tattccattg
     5821 ctgtgaaaaa ctggggtcat tcgggatgaa ttccctcagt tcgatgaaat ggttgaactg
     5881 ggccagttcg acaaacggat gggatttgat ctcctggagc atccggtcgt cactgatcac
     5941 cgacggatcg tagctgagga ggaagatatt caggcggtcg gagagccggc ggataacgcc
     6001 aagccctgcg ccctggaaat cccccagcac atcctgcagc atttgctgct ggttctgggg
     6061 aaggtcgctg tatagcttga tcatgatttc accttcggca tagggttttt cgtgcttgtt
     6121 ggcgggggta ttggatataa ggaatggtat gataatcagg aagataaaga aagatgtgat
     6181 ttttttcata agacagggaa tttgaggggg taaagataag attatttctg atgatgggtg
     6241 ttgggtttat tcaccacaga gtaggaagtt gtctcaaaag tccatatatt cacttttatt
     6301 taggtagtcc gacgagcgtc ccgtcgat
//
LOCUS       DAOQXS010000102         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12727_length_6305_cov_3.076320, whole genome shotgun sequence.
ACCESSION   DAOQXS010000102 DAOQXS010000000
VERSION     DAOQXS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12727_length_6305_cov_3.076320"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PKM34_04180"
     CDS             <1..1292
                     /locus_tag="PKM34_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82817.1"
                     /translation="FPIIILTWITLPFGGYCQDFWEPIPYPNSTESMSCFAMDGYGRL
                     FACNVDDVFYTDDMGVNWYYTTNWPDQYPRCMVTNASDDVFIVTYSGNIFRSTDRGVS
                     FHAVSNGLTADFLRCLIVLDNDVLLLGTSEGIFRSVDNGDFWEPYGTGLPDGWIDELS
                     AGADGKIFAGTQLSGVYRSYDYGATWTAANNGLPAEAQVTSILTVPGETSAYAGLYPQ
                     GMFFSSDDGDSWAEHNEGLPFDKDLLKGNRDYSAHGLTYLQQMVFIYIYSQVIYYALQ
                     YSVPPSPWYSLISGLPPDSQINALRAVLATILVCATLSSNDVPLAKDGPGIYVNAVPV
                     NINAYAAPSSVYQLSIVPNPVSGSSNIRINMPEKGRAILSLCNQLGQEIMRIADQELS
                     EGIHDFNLNALTLDPGLYFLKLISKNEMISTRVLSLN"
     gene            1361..1888
                     /locus_tag="PKM34_04185"
     CDS             1361..1888
                     /locus_tag="PKM34_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNQ82818.1"
                     /translation="MMMKKIALIYWPKKGSTETAAHKIHAKFDPGMIDIFTITEVNTA
                     EFELYDAFIIGGSTTGADNWESAHKTRWSDFFAKLEKAEIKGKPFALYGLGNQVLYPN
                     HFVDGMAFLKEKFEKRGAVLKGRWPVDGYDFEESESVENGLFYGLALDFDTQDELTDE
                     RIAKWTGQIRKEFGI"
     gene            complement(1892..3367)
                     /locus_tag="PKM34_04190"
     CDS             complement(1892..3367)
                     /locus_tag="PKM34_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ82819.1"
                     /translation="MILSKKIITFLKLFSSFFPLFLITIFFTSCLTDHQGKIKPIRAG
                     EDSSRKLRIHEIRERGKLIATTNYNSTDYFIYRGEPMGYQYEKLKMFADYLDVELEIK
                     VALNLDEAFTSLEEGKADVIAMGLAQTRDRVEKYDFSSPIFQTRQMLVQRKPDNWRKM
                     RTWDDIEKSLIRNPLDLAGKTVIVQKSSAYAGRLENLSEEIGAPINVQVDPLMEAEQL
                     VEAVAKGEIDFTVCDEHMAAFFERQHSELDIRTPISFPQNIGWVVRHDSDSLLAAIND
                     WLGESESTLASRHLMDKYFNNPRASFMARSEKINMGGKQISEYDEILRNISRQHDFDW
                     RLVASLVYQESQFQPEVKSWAGAFGLMQLMPATAKLFGIDSTASEFQQIEAGVMFMKK
                     IDDELPRDIKDRSERIKFILAAYNVGIAHVMDARRLAEKHGKDPNVWTGNVDYYILNK
                     SNPKYYRDSVVRYGYARGEETYQFVNEVLDRFEHYKNYVGD"
     gene            complement(3543..6029)
                     /locus_tag="PKM34_04195"
     CDS             complement(3543..6029)
                     /locus_tag="PKM34_04195"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNQ82820.1"
                     /translation="MTKFYKQKLLLLLLMFGPVGLVLAQDFLPTMSDNYMGINQVVLQ
                     PASISDSRFMVDINVFGANSDIYNDALRLRSKWILNPSGYRNNENWWSENTYLDNPDG
                     LDKHMFSSQSILGPGFMISLPYSQAIGFTSRVRSITNVDGISEPLIRTVQSDYTNENY
                     WNQWVFDDHMRAVQHVFADYGLTYAATILNTGPHVLKGGVTVKLLQGIAASYIQSDSL
                     YYYFDGENNDGDYPMSYNASHIYSGVSDNWGDDNEAGIFDYSMNFQYTSKPSAGLDVG
                     FVYEFRPKYLDFRYDMDGEKDLERNDKNKYLLKVGASIVDIGRIKYKKEYNSFDFSAN
                     FTPDYADRYQNGDNSVPDNTYWLNAEVGSFSFQKYVNFVDTMYHRQLSGRGVGKVDGN
                     DEKFMVTLPTALSLQIDVRVVEGFYVNLTSFSGLHQGFSRAPNSHQMSNYSITPRYER
                     KWFTISMPFQYNQFQKMNVGLGFRAGFIYAGVTNLFSGLIKDPYGTNIYLGAKIPLFK
                     KGSPPDKDNDGVSDEKDICPDIPGPWQFYGCPDRNGDGVADSEDVCPDIAGKKEFLGC
                     PDTDGDGVSDKDDKCPKVPGPKITNGCPDFDNDGVVDDQDECPEIAGPAKFAGCPDSD
                     GDGIPDIKDNCPDLAGLAEYAGCPYLDTDGDGVKDADDMCPDKKGPPENNGCPYSDSD
                     GDGVVDKEDRCPLTPGDPANFGCPILAVEDAEILNTAFQNLEFETAKAVIRSSSFPSM
                     DELARLLISKPSYKLKISGHTDNVGSDAYNMTLSKNRAQSTARYLIGKGVPQNRLLTD
                     WFGESQPIADNNTPEGRQINRRVEMQVVFD"
BASE COUNT         1619 a         1511 c         1466 g         1709 t
ORIGIN      
        1 tgtttcctat tatcatttta acctggatta ctttgccctt cgggggatat tgccaggatt
       61 tctgggaacc gatcccttat cctaactcca ccgaatcgat gagttgcttt gctatggacg
      121 gatatggacg gctgtttgcc tgtaatgtgg atgatgtctt ttataccgat gatatgggag
      181 tcaattggta ttacacaacc aactggcccg atcagtatcc cagatgcatg gtaaccaatg
      241 catcggatga tgttttcatc gttacatact cgggtaacat ctttcgctca accgacaggg
      301 gtgtgagctt ccatgccgtt tccaacggtc tgaccgccga tttcctcaga tgtctgatcg
      361 tactcgataa tgatgtcctt ttgctgggta cttctgaagg tattttcagg tcggtcgata
      421 atggggactt ttgggaaccc tatggtacag gcctccccga tggctggata gatgaactct
      481 cggcaggcgc tgatggaaaa atatttgccg gaacacaatt atcaggcgtc tatcgttcct
      541 atgactacgg tgcaacctgg accgccgcca acaatggcct tcccgctgaa gctcaggtaa
      601 cctccatact aaccgtaccg ggcgaaacct cggcttatgc aggcctttac cctcagggca
      661 tgttcttctc ttccgatgat ggcgactcct gggcagaaca taatgagggc ttaccctttg
      721 ataaggacct gttgaaagga aatcgcgact attctgccca cggtcttaca tatctgcagc
      781 aaatggtgtt tatctatata tattctcagg ttatttatta tgccttacaa tattctgttc
      841 cgccaagtcc gtggtatagc ctgatcagcg ggcttccgcc cgacagccag atcaatgcct
      901 tacgtgcggt ccttgccaca atattggttt gtgccacgct tagcagcaat gatgtgccat
      961 tggctaagga cgggccgggg atatacgtca atgcggtccc ggtaaatatt aatgcatatg
     1021 ctgctccatc ttcagtatat cagctttcca tagtgccaaa tccggtctcg ggttcttcca
     1081 acatcaggat aaacatgcct gaaaaaggtc gtgcaatcct ctccctctgt aatcaactgg
     1141 ggcaagagat aatgcgaatc gctgatcagg aactgtcgga aggcattcat gatttcaacc
     1201 ttaatgctct tactctcgat ccgggattgt atttcctgaa actcatatca aaaaatgaaa
     1261 tgatttcaac ccgggtttta agcttgaatt gaagaaaacc gtaaaacgca aaacagaaca
     1321 agcgacattt tccgtatttt tgccatcatt cttaatttat atgatgatga aaaagatcgc
     1381 tttgatttac tggccgaaaa agggaagcac ggagacggcc gcacacaaaa tacacgctaa
     1441 attcgatccg gggatgatcg atatcttcac gatcaccgag gtcaatacgg ccgaatttga
     1501 gctgtacgac gcttttatca tcgggggctc aaccaccggc gccgacaact gggaaagcgc
     1561 ccacaaaacg cgctggagcg acttcttcgc caaactggaa aaagcggaaa tcaaagggaa
     1621 acctttcgcc ctctatggcc tgggtaacca ggtcctctac ccgaaccact ttgtcgacgg
     1681 gatggcgttc ctgaaagaga aattcgagaa aagaggcgcc gtgctgaaag gccgctggcc
     1741 tgtcgatggt tatgatttcg aagaatcgga atcggtcgag aacggattgt tttacggcct
     1801 ggcactcgat ttcgacacgc aggatgaact tacggacgaa cggatcgcca aatggaccgg
     1861 ccagatccgt aaagaatttg gcatctagcg gttaatcgcc gacgtagttc ttgtaatgct
     1921 cgaagcggtc gagcacctcg ttcacaaact gatacgtttc ttcaccgcgg gcatagccgt
     1981 atcgtaccac cgaatcgcgg taatatttcg ggttcgactt attgaggata taataatcga
     2041 cgttacccgt ccatacattc gggtctttcc cgtgtttttc cgccagcctg cgcgcatcca
     2101 tcacatgggc gatacccaca ttgtaggccg ccaggatgaa tttgattcgc tcggagcgat
     2161 ctttaatgtc gcgcgggagc tcgtcgtcga tctttttcat gaacatcacc ccggcttcga
     2221 tctgctgaaa ctcggaggcg gtgctgtcga ttccgaatag ctttgccgta gccggcatga
     2281 gctgcatcag gccgaaagcg ccggcccagg atttgacctc gggctggaac tgcgactcct
     2341 ggtaaaccag cgaggccacc aggcgccagt cgaaatcgtg ctggcgggag atattgcgca
     2401 aaatctcgtc gtattcggag atctgtttcc cgcccatgtt gattttctcg ctcctggcca
     2461 taaaggaagc acgcggattg ttgaaatact tatccatcaa atgccgtgat gccagtgtgc
     2521 tttccgattc gcccagccag tcgttgatcg cggccaggag gctgtcggag tcatgcctga
     2581 ccacccatcc gatattctgc ggaaagctga tcggggtccg gatatcgagt tccgaatgct
     2641 gccgctcaaa aaaggcggcc atatgctcgt cgcaaacggt gaagtcgatc tctccctttg
     2701 cgacggcctc gacaagctgt tcggcctcca tcagcgggtc gacctgaacg ttgatcggcg
     2761 caccgatctc ttccgagagg ttttcgagac ggccggcata ggcggaagat ttttgcacga
     2821 tgacggtttt accggcgaga tccagcgggt tgcggatcag cgatttctcg atatcgtccc
     2881 aggtgcgcat tttacgccag ttgtcgggtt tgcgctgcac cagcatctgc cgggtctgga
     2941 aaatcggtga tgaaaaatcg tatttctcga cgcgatcgcg ggtttgggcc aggcccatgg
     3001 caatgacgtc ggctttgcct tcttccaggc tggtaaatgc ttcgtcgagg ttaagcgcga
     3061 cttttatctc cagttccaca tcgaggtaat cggcaaacat cttcagcttt tcgtactggt
     3121 atcccatcgg ttcaccgcgg tagataaagt agtcggtaga gttatagttg gtcgtggcaa
     3181 tcagctttcc acgctccctg atctcgtgaa tacgaagctt acgcgacgag tcttctccgg
     3241 ctcgtatcgg ttttattttt ccctggtgat cagtcaggca ggaagtaaag aagattgtga
     3301 ttaaaaatag agggaaaaaa gaggaaaaaa gctttaagaa ggtgattatt tttttagata
     3361 agatcatcta ttctgttttc gttcaacact ggccttccgt taaatggcac tgcaagcgcc
     3421 tctggatcag gcttttattt aaagatatta agttattgga ttagcgtcgg caaaagtaag
     3481 taaaaattcc atccggttta catccggatg gaatcttatg aatatcagtt tgttaaaaaa
     3541 tgttaatcaa acaccacctg catctcaacc cttcggttga tctgacggcc ttcaggtgta
     3601 ttattatcgg ctattggctg gctttcacca aaccaatcgg tgagaagtct gttttgcggc
     3661 actcctttgc cgataagata acgggcggtc gactgcgccc ggtttttcga cagggtcatg
     3721 ttatacgcat cgcttccgac gttatcagta tgtcctgaaa tcttcagttt ataggatggt
     3781 ttgctgataa gcagtctggc aagctcatcc attgagggga atgaagaaga gcggatcact
     3841 gcttttgccg tttcaaattc caggttttga aaagcggtat tcaggatttc agcatcttca
     3901 acggcaagga tcgggcagcc gaaattagcc ggatcaccgg gtgtcagcgg gcagcggtcc
     3961 tctttatcga ccactccgtc gccatcagaa tcagaatagg ggcaaccatt attttcaggt
     4021 ggtcctttct tatccgggca catgtcatcg gcatccttta cgccatcgcc gtcggtatcg
     4081 aggtatgggc aaccggcata ttcagccaga ccggccaggt caggacaatt gtctttaata
     4141 tccggaattc catcaccatc actatcagga caacctgcaa atttcgcggg cccggcgatt
     4201 tccggacatt catcctggtc atcgaccaca ccgtcgttat caaagtcagg acatccattg
     4261 gttattttgg ggccggggac tttcggacat ttgtcgtcct tatcagagac accgtcgcca
     4321 tcggtatccg ggcaacccaa aaattccttc ttgccggcta tatcaggaca aacgtcttca
     4381 ctatcagcca cgccgtcgcc attacgatcg gggcagccat agaattgcca tggaccggga
     4441 atatccgggc aaatatcttt ctcatcagat accccatcat tgtctttatc tggtggtgaa
     4501 cccttcttga acaggggaat tttggcgccc aggtaaatat tcgttccata aggatctttg
     4561 atcaaacctg aaaacaggtt ggtaacaccc gcatagatga aacccgccct gaaacccagc
     4621 ccgacattca ttttctggaa ctggttgtac tggaaaggca tagatatggt gaaccattta
     4681 cgctcatagc gcggggtaat gctgtaattt gacatttgat gcgaattcgg tgctcttgaa
     4741 aacccctggt gcaatcctga gaatgatgtc aggttgacgt aaaatccctc taccacgcgt
     4801 acatcgatct gcaggctaag tgcggttgga agcgtgacca tgaatttttc atcattacca
     4861 tccacttttc ctactccacg tcctgagagt tgtctgtgat acattgtatc gacgaaattc
     4921 acatacttct gaaaactaaa actaccgact tctgcattta accagtaggt gttatctgga
     4981 acgctattat ctccgttctg gtaacgatca gcataatccg gcgtaaaatt ggctgaaaag
     5041 tcaaaactgt tatattcttt tttatactta attctgccaa tatccacaat agaagccccg
     5101 actttcagca aatatttgtt cttgtcattt ctttccagat ctttttctcc atccatatca
     5161 tagcgaaagt ccagatattt tggacggaat tcatatacaa aaccaacatc cagaccggcg
     5221 gacggctttg atgtatattg aaaattcata gaatagtcaa atattcctgc ttcgttatca
     5281 tctccccagt tatccgacac tccactgtaa atatgcgaag cattgtacga catcggataa
     5341 tccccgtcat tattttcgcc atcaaagtaa tagtagaggc tgtcactttg aatataagaa
     5401 gcggcaatac cctgcagcag cttgacggtg actcctcctt taagaacatg agggcccgta
     5461 ttcagaatcg tggctgcata ggttaagccg taatcggcaa atacatgctg aacggcacgc
     5521 atatgatcgt cgaaaaccca ttgattccag tagttctcat tggtatagtc gctttggacc
     5581 gttcgaatca ggggttcact gatgccatcg acattggtga tgctacggac acgtgatgtg
     5641 aagccaatag cctgactata tggcagggag atcataaagc cgggccccag aattgattgt
     5701 gatgaaaaca tatgtttatc caatccgtcg ggattatcaa ggtaggtgtt ttcactccac
     5761 cagttttcgt tgtttcggta accggatgga ttcaggatcc atttagatcg taaacgcaaa
     5821 gcatcgttat aaatatcact atttgcacca aaaacattga tatctaccat gaagcgactg
     5881 tcggaaattg aagccggctg taaaacaacc tgattgatcc ccatgtagtt atcgctcatt
     5941 gttggaagga agtcttgtgc taaaaccagg ccaaccgggc caaacattaa taatagcaac
     6001 agcaactttt gtttgtagaa ttttgtcata atacctaagt ttaaagggtt ataagattgt
     6061 attagaaatt gctcagattc aacctgcaaa ggtcaatctt atatatacgg aaagcgttac
     6121 acaattaaaa ataagagtta tataattcac acattgtcag gcaaaaagaa attttcacct
     6181 gaaactccga taagaataat ttgaaattgg acccgaaatc tatcagaagg ccaggatatc
     6241 attatcgaca tcggcgcggg gtttaccttt gaaaacgggg aattttgcgc cgatatatac
     6301 attca
//
LOCUS       DAOQXS010000103         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12808_length_6268_cov_3.766618, whole genome shotgun sequence.
ACCESSION   DAOQXS010000103 DAOQXS010000000
VERSION     DAOQXS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12808_length_6268_cov_3.766618"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKM34_04200"
     CDS             <1..831
                     /locus_tag="PKM34_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNQ82821.1"
                     /translation="GSGSGSVLDSEKFLLSIYKNNENLLPPTPFIQSTHNAVGAQVAM
                     LLGNCSYNMTYAHGIFAFEHAMLDAMLCFMEGGKNNILLGGFDEITDNQFILTDRLGR
                     WRKEPVQSLEMIDRKSKGTVAGEGYSFFFLQDHPSSTTYARLTGVRTIYRTDNKDDVS
                     VAIIKFLNDHGLKPGDISCIFAGYNGDEQGDLYYDDVLARVFRKGVPEARWKHLTGDY
                     LTAGAFATWAACQSIKAQTIPASLLSSGHPQAPIRYILIYNHFNCAHHSLILISHPDE
                     "
     gene            840..1940
                     /locus_tag="PKM34_04205"
     CDS             840..1940
                     /locus_tag="PKM34_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ82822.1"
                     /translation="MNKRLYLPKFPHQFTFNLGLRIRLMLTRLTDRLVPAPMAVYEKA
                     QGIWISGAIGTACELNIADHLAPGPKSIMELARKTNADEDFLYRLMRALAGEGIFKEM
                     PGKVFANNRLSLPLADGEKSMKYMIRHQFGETNMTLFLHLTESIREGQGNSQKFLGKR
                     VFQYLQDHPLNNDIYNQAMNSSSNLVAVALLSAYRFKGIKTLVDVGGGQGVLLKLIIE
                     KHKHIRGILYDQKHVTQKADIVLKESSDNGKIEIVSGNFFTDVPAGADGYFMKNILHA
                     FHDTDCIRLLKKINEVMPDHGKVIILENVTFPDNKPSFGKRLDLMMMTGTDGGKERTR
                     EEFESLLDRSGFRLKRFIRTISPFFVLEAIKK"
     gene            2053..2439
                     /locus_tag="PKM34_04210"
     CDS             2053..2439
                     /locus_tag="PKM34_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82823.1"
                     /translation="MKEKDLYIESKEQQMILYAEKEDESYGEIATGSYATKHHLPEFI
                     KYKETLDKELRKELMAGKISPLYYFMLMQDMGPGDLAKRVGVNRWKFKKQMKPENFDK
                     LGDKILEKYALVFGVSIERIKELKTH"
     gene            2440..3450
                     /locus_tag="PKM34_04215"
     CDS             2440..3450
                     /locus_tag="PKM34_04215"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNQ82824.1"
                     /translation="MDTREIPFEHRMAAHCETGTVTALLNHGGLTLTEPLVFGIAGGI
                     FFGYLKSPDLPFPMFILRSKPGNIRRKISKRLGIKFAESRFKNPSEGEAALDKAIRDG
                     HPTAVQVDFFYMDYMPEWQRVHINAHYVIVCGVNGSHYTVSDSYYKQKSLLERQKMLT
                     ARFAGGMMAPKGMMFYTDHIPEKIDLTKPIITGIKRASFNMTRLPIPFLGIKGIHRFA
                     EKVKEWPALTRDADELADRIMRINILLEDQGTGGGGFRYMYATFLQQAAPIVNDDRLK
                     SFSKEMMEIGDKWRNISYFAAKISKNRDLGPERFEELSRLIHAQGDEEQKFFSELYKL
                     VK"
     gene            3463..3741
                     /locus_tag="PKM34_04220"
     CDS             3463..3741
                     /locus_tag="PKM34_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNQ82825.1"
                     /translation="MTAEETTLLKTEIKELIIQTLNIKNIKAEQVPDAVPLFSPENIL
                     ALDSIDGIELVMAIQEKYQIRIADQNVARNVLESVDSIAAFVIREKGL"
     gene            3751..4830
                     /locus_tag="PKM34_04225"
     CDS             3751..4830
                     /locus_tag="PKM34_04225"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNQ82826.1"
                     /translation="MHSYILTGIRIGPLMRLLRDNGFTPSPKNIGRLIFVFQNGLWSS
                     ILARREKKLHGRNISQWPVPDDPLIIVGHWRTGSTFLHQLLSCDDRFISPTLYQCSFP
                     ESFINGERFYRPVMGAMVRKRPIDNMDMGFDDAQEDEFAWVKLAPGSPFLKFVFPDGQ
                     GYFVNAADDFIPPPPVRRIWKEQMQQFGAKICRDTGKRLLLKNPFHSLRIPFIREVFP
                     NAKFIHLHRHPYEVVASSLSLWKVVARDNQLKGKPGIPGTEEVAEGLQKFYSVIKRDL
                     AGLPENNYFEISYESLVKDPILELNRLYVRLGMDFSDAARENMAARLQKVSNFRKNTY
                     TFDDSQKELVYRVLRKEFEQYHYPV"
     gene            4832..6097
                     /locus_tag="PKM34_04230"
     CDS             4832..6097
                     /locus_tag="PKM34_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNQ82827.1"
                     /translation="MILRLIYPRWTRLEGQNTFILPPLGPTAFAAALPGDITIDFIDE
                     NLEPVNFDDPCDLVGISMMLTAQVKRGWEIADEYRGRGKKVIFGGISTMLHAQETIPH
                     ADSVFLGEVEGRMEEVIGDFLRGELKQVYNYLPHPPDTALIGPARRHILHEDLYYHKG
                     IRMVDLFHASRGCRFACYPCAASFLGGRRFRPRPFDKVAEELDAIKNNRLFIVDNSLA
                     QDKEWEKELFRMMIPFKKNWCSHTIDDDPEILDLAAQAGAWYVYQAVIDDSDYIRKRI
                     QRYHDFGIAVEATVLLGMDDQTEDDILRLIDFLQEVNLDLAEFTVLTPFRHTKVWDDM
                     ERDGRIIDRDWSNYNAGKVVFKPRHMSPDRLQELYHYAWEKFYEHENQEQKMFKMIMK
                     VIERERKQGTYQPPRKDLARMSFGQIIDR"
BASE COUNT         1678 a         1567 c         1603 g         1420 t
ORIGIN      
        1 ggctcgggct cgggcagcgt cctcgactcc gagaaatttc ttctgtccat ttataaaaac
       61 aacgaaaacc tcctcccgcc gacgcctttc atccaatcaa cccacaatgc cgtaggggcg
      121 caggttgcca tgctccttgg caactgttcc tacaacatga cctacgcgca cggcatcttt
      181 gctttcgaac acgccatgct cgatgcgatg ctttgcttta tggaaggcgg gaagaataat
      241 atcctcctcg gcggcttcga tgagatcacc gataaccagt tcatcctgac cgacaggctg
      301 gggcgctggc ggaaagaacc cgtgcaaagc ctggaaatga tcgaccggaa gagtaaagga
      361 acggttgctg gcgagggtta ttccttcttc ttcctgcagg atcatccatc gtcaacgacc
      421 tatgcccggc tcaccggggt aaggacgata taccgaacgg ataataaaga tgatgtgtcg
      481 gtcgccatta taaagttcct taatgaccac ggtctgaaac ccggagatat cagttgtatt
      541 tttgccggct acaacgggga tgaacaggga gatctatatt acgatgacgt cctggccaga
      601 gtcttccgaa agggagtgcc ggaagcccgg tggaagcacc tgacgggtga ttatctgacg
      661 gccggcgctt ttgccacctg ggcggcctgc cagtcgatca aagcgcaaac gattccggca
      721 tcattgttaa gttccggcca tccccaggct cccatcagat atattttgat ctataatcat
      781 ttcaattgcg cacatcacag cctgatattg atttcgcatc ctgatgaata aaggaaatta
      841 tgaataagag actatatctg cccaaatttc cccaccagtt tacttttaac ctgggattac
      901 gcatcaggct gatgctgaca aggttaaccg acaggctggt gcccgctccc atggctgttt
      961 atgaaaaggc gcaggggata tggatttcgg gcgccattgg aacggcttgc gaactgaata
     1021 tcgccgacca cctggccccg gggcctaaaa gcatcatgga gctggcccga aaaaccaatg
     1081 cagacgaaga ttttctttac cggttgatgc gggcgctggc cggtgaaggc atatttaaag
     1141 aaatgccggg aaaagtattt gcgaataacc gcctctcttt accacttgcc gatggtgaga
     1201 aatccatgaa atatatgatc aggcaccagt ttggcgaaac caacatgacc ttgttccttc
     1261 atctgacaga atctatccgg gagggacagg gaaactcaca gaagttcctt ggaaagaggg
     1321 tattccagta tcttcaggat catcctttaa acaatgacat ttacaaccag gctatgaaca
     1381 gttcttctaa cctggtagca gttgcccttc tgtcggccta ccggttcaag gggatcaaaa
     1441 cactggtgga tgtaggtggc ggccagggag tccttctgaa actgatcatc gaaaaacaca
     1501 aacatatccg gggaatcctt tacgaccaga agcacgttac ccagaaagcg gacatagtgc
     1561 tgaaggaatc ttctgacaac gggaagatag aaattgtcag cggaaatttt ttcactgacg
     1621 tgccggccgg ggccgacggt tatttcatga aaaacatcct gcatgctttc catgataccg
     1681 attgcatcag gctgctgaaa aagatcaacg aagtaatgcc tgaccacgga aaagtcatta
     1741 tcctggaaaa tgtgacattt cctgacaaca aaccctcctt cgggaagcgg cttgacctca
     1801 tgatgatgac cggaacagat gggggaaaag agcggacccg ggaagaattc gaatccctcc
     1861 tggaccggtc cggattccgc ctgaaacggt tcatccggac gatttcgcct tttttcgtcc
     1921 tggaagccat caaaaaataa tttcattttc atcttcttat cataaaaaaa aactcgctta
     1981 tttttgtcgt caggataaat gatccggata ttccacccgg taaaaatctc cctcaaatga
     2041 aaaaacgcta agatgaaaga gaaggatctc tatatcgaat ccaaagagca gcagatgatc
     2101 ctttatgccg aaaaggagga tgaatcctac ggtgaaatag ccaccggatc ctatgctaca
     2161 aagcaccatc ttcctgaatt tataaagtat aaagaaacac tcgacaagga attgcgtaaa
     2221 gaattaatgg ccgggaagat cagccctttg tattatttta tgctgatgca ggatatgggg
     2281 cccggggatt tggccaagcg agtcggggtt aaccgatgga aatttaagaa acagatgaaa
     2341 ccggagaatt tcgacaaact cggcgataag attctggaaa aatacgctct cgttttcggc
     2401 gtgagcattg agcggatcaa ggaactcaaa acacactgaa tggacacacg ggagatacct
     2461 tttgagcaca ggatggcggc tcattgtgag acgggaactg ttacggcatt attaaatcac
     2521 ggcgggctta cgcttacaga gcccctggta ttcggaatcg ccggcggcat ttttttcggc
     2581 tacctcaaat cgcccgacct gccctttccc atgtttattt tgcgaagcaa gccgggaaat
     2641 atccgaagaa aaatttcaaa acgattaggg ataaaatttg ccgagagcag gttcaagaat
     2701 ccgtctgaag gagaagcggc gctggataag gccatccgcg acgggcaccc gaccgcggtc
     2761 caggtcgatt tcttctatat ggactacatg ccggaatggc aaagggttca tatcaacgcg
     2821 cactatgtca ttgtttgcgg ggtgaacggc agtcattaca ccgtcagcga cagttattac
     2881 aagcaaaaat cgctgcttga aaggcagaaa atgctgacag cccgttttgc cggcggaatg
     2941 atggcgccta aaggcatgat gttttatacc gaccacattc ctgaaaaaat cgatctgacg
     3001 aaacccatca tcaccgggat aaaaagggcc tcttttaaca tgacgcggct gcccatcccc
     3061 ttcctgggga taaaaggcat tcacaggttc gccgaaaagg tgaaagagtg gccggcattg
     3121 acccgtgacg cggatgagct ggccgaccgg atcatgcgga tcaatatcct gctggaggac
     3181 caggggacgg gcggcggcgg cttccgctac atgtacgcca cgttcctgca gcaagccgcc
     3241 cccatcgtca atgacgaccg gttgaaatcg ttttccaaag aaatgatgga aattggcgat
     3301 aagtggagga atatttctta ctttgcagcg aaaatttcaa agaaccgcga cctgggaccg
     3361 gaacgtttcg aggaactgag ccggctgatc catgcccagg gcgatgaaga acagaaattt
     3421 ttcagcgagt tgtataaact tgtcaaataa acctaataaa ggatgacggc ggaagaaacc
     3481 acattgctga agaccgagat caaagagctc atcattcaga ctctcaatat aaaaaatatc
     3541 aaagcggaac aggtacccga cgctgttccc ctgttttctc cggaaaatat actggcactc
     3601 gattccatcg acggcatcga actggtgatg gctatccagg aaaagtacca gatccggatc
     3661 gccgatcaga atgtggccag gaatgtactt gagtcggtcg attccattgc tgcattcgtc
     3721 atcagggaaa aagggctctg atcctgcctc atgcattcct acatcctcac cggaatccgt
     3781 atcggacccc tgatgcgtct tttgcgtgac aacggtttta ccccctctcc caaaaatatc
     3841 ggacggctga tctttgtttt ccagaatggc ctctggtcgt cgatcctggc acgcagagaa
     3901 aagaaactgc atggccggaa cataagccag tggccggttc ccgacgaccc gctgatcatc
     3961 gtcggacact ggcggaccgg cagcactttc ctgcaccagt tgctgtcgtg cgatgatcgg
     4021 ttcatctccc ctacccttta ccagtgctcc ttcccggaat ctttcatcaa tggtgaacgt
     4081 ttttaccggc cggtgatggg agcaatggtc agaaaaagac cgatcgacaa tatggatatg
     4141 ggattcgatg atgcccagga agatgagttt gcctgggtca aattagcgcc gggctcacca
     4201 tttctgaaat tcgtgttccc ggatggccaa ggttattttg tcaatgcagc cgatgacttc
     4261 ataccgccgc ctcctgtgcg tcgtatctgg aaggaacaaa tgcaacagtt cggggcaaag
     4321 atatgccgcg acaccggcaa gaggctgctg ctgaaaaacc cgttccattc cctgcgaatc
     4381 ccttttatcc gggaggtttt cccgaatgca aagtttatcc atcttcaccg gcatccctac
     4441 gaggtggtgg cttcttcctt aagtctctgg aaggtggtgg cccgtgacaa ccagttgaaa
     4501 ggcaaacctg gtatccccgg cacggaagag gtagccgaag ggctgcagaa gttttacagt
     4561 gtgattaaaa gggacctggc cgggttaccg gaaaataatt attttgagat tagttatgaa
     4621 tcgcttgtga aagatcccat tttggagtta aatagacttt atgtaagatt aggaatggac
     4681 ttttcggatg cagccaggga aaatatggcg gcgcgtctgc aaaaagtcag caatttcaga
     4741 aaaaatacct atacatttga cgacagccag aaggagctgg tttaccgggt cctgagaaaa
     4801 gagtttgaac aatatcatta ccccgtttaa tatgatcctc cgtttgatat atccccgctg
     4861 gacccggcta gaagggcaga acacctttat actaccgccc ctggggccca cagcatttgc
     4921 cgccgcctta cccggcgata tcaccatcga tttcatcgat gaaaacctcg agccggtcaa
     4981 ctttgatgat ccctgcgacc tggtgggcat ttcgatgatg ctgactgccc aggtcaagcg
     5041 cgggtgggag attgccgacg agtaccgcgg ccggggaaag aaggtcattt tcgggggtat
     5101 ttcgacgatg ctgcacgcgc aggaaacgat cccccatgcc gacagcgtct tcctgggcga
     5161 ggtggagggc cggatggaag aggtgatcgg cgatttcctc cgcggagagc tgaagcaggt
     5221 ctataactat ctaccccatc cgcccgatac ggctttgatc gggccggccc ggcgccacat
     5281 cctccatgaa gacctctatt accacaaagg catccgcatg gtcgatctgt tccacgcatc
     5341 ccgcggttgc cgctttgcct gctatccctg cgccgcttca ttccttggag gacggcggtt
     5401 caggccccgg ccgttcgaca aggtggccga agaactcgac gcgatcaaaa acaaccggct
     5461 gtttatcgtc gataattcgc tggcacaaga taaagagtgg gaaaaggaac ttttccggat
     5521 gatgatccct ttcaaaaaga actggtgcag tcacaccatc gacgacgatc cggaaattct
     5581 cgacctggcg gcacaggccg gcgcctggta tgtgtaccag gctgtgatcg acgattccga
     5641 ctacatccgg aagcggatcc aacggtacca cgatttcggc attgccgtgg aagcgaccgt
     5701 gctcctgggg atggatgacc agaccgaaga cgatatcctc cgcctgatcg acttcctgca
     5761 ggaggtaaac ctcgacctgg ccgaattcac ggtgcttacg cctttcaggc ataccaaagt
     5821 gtgggacgat atggagcgcg acggccggat catcgaccgc gactggtcga actacaacgc
     5881 cgggaaggtc gttttcaagc cgcgccatat gtcgcccgac cggttgcagg aattgtatca
     5941 ttacgcctgg gaaaaatttt atgaacacga gaaccaggaa cagaaaatgt tcaagatgat
     6001 catgaaggtc attgaacggg agcggaaaca aggcacctac cagcctcccc ggaaggacct
     6061 ggccaggatg agtttcggac aaattatcga ccgctgaaat atggataatc aggataaata
     6121 cacccgggag gtcttctatt tttcaggaca gtgggaaatg ccatcccact gcggaatcct
     6181 gaaggaggaa agagatggga tgacttatgt aatccttacg gagatgtacg agacaaatcc
     6241 cggacgctcc gtcaccagca tgatcgaa
//
LOCUS       DAOQXS010000104         6253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12846_length_6253_cov_3.822846, whole genome shotgun sequence.
ACCESSION   DAOQXS010000104 DAOQXS010000000
VERSION     DAOQXS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12846_length_6253_cov_3.822846"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKM34_04235"
     CDS             <1..564
                     /locus_tag="PKM34_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNQ82828.1"
                     /translation="QTGELIADSATVLGVNGTPTFGFNIGIVGDKRLGRYFNVRFVPA
                     LVFGERYLNYSVRAWANGEEKLVDIKKNIGSVFIDFPFTIKYKSKRHNNYLAYLLAGG
                     KYTLDIASNAKKKDSNGQVVVKLNKHDIYAIVGVGFDFYNPWFKMGVELKMSYGLLDV
                     LKDDKTFFTQSIDKINSKVFELGVTFE"
     gene            582..1262
                     /locus_tag="PKM34_04240"
     CDS             582..1262
                     /locus_tag="PKM34_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82829.1"
                     /translation="MSPLQNAGLYQIVNNKGKLKKSVYHNTLESLNLIKKTIGEMLED
                     YRREFAKDHEAIPFVVKDMGEFEIHLQFAGDVLIFNMHTNVFEFSRYHDVMSTSYIRE
                     DKARSYCGIINIYNFLADSFKYDRANDIGYLIGRIFINKDKSYFIEGKREVGLLYNNF
                     GQEKLDETAVRKVVESAVLYTINFDLLTPPFDSVKEISLSEMKSIAGSQTLKTGKRLG
                     FKFQADRD"
     gene            1335..1411
                     /locus_tag="PKM34_04245"
     tRNA            1335..1411
                     /locus_tag="PKM34_04245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1369..1371,aa:Glu,seq:ttc)
     gene            1557..2315
                     /locus_tag="PKM34_04250"
     CDS             1557..2315
                     /locus_tag="PKM34_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNQ82830.1"
                     /translation="MEKIVDNQYYDAAIEANMAVVLLKTRAFDLITSVPDSDELMEFI
                     RDAEHNPKVKALMLLNEPDCLGEKVYDEFIRSILKSDQNSENSDVPAFLQKNTRFRQI
                     INLNKLIRYLAGYQKLVVVGVSSTIVTPFMGVALVADLRLASPKGCFALAHTRYGLHP
                     SGAIPFFLTHYLGHSKALEVQLSERVCAEEAFRLGIVNQILPSEEFYSNCIRFVQPYL
                     QHCRSTLAMTKRLNNFKNRSLQEYLDHEASLMNL"
     gene            2462..3025
                     /locus_tag="PKM34_04255"
     CDS             2462..3025
                     /locus_tag="PKM34_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ82831.1"
                     /translation="MVHYTKYGIIEGIRRKDKDVLRYVYKQYFPMVRYFIVRNNGTEQ
                     DAEDIFQEAMIVVFERARKRNLALGCAFKTYLYSVVRHIWLQLADRNRLQYELSDMDE
                     FLLLEEKEIYDDFHYKKVIFQRKFLELTELCRKLLLMVVEGKPYEEIATILGYKGKQY
                     AVKRKYECMKSLINRVLNDPEYKNLTK"
     gene            3022..3699
                     /locus_tag="PKM34_04260"
     CDS             3022..3699
                     /locus_tag="PKM34_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82832.1"
                     /translation="MKTKYLKLFERYYNGEMDEPEKEMFQSQLQNDHEMADAYREYLS
                     IYEALKDKETLEFRTKLNEIRDEFRHNRKKDRFMNDKTNWLLVAALFILIAGITVITS
                     FFVLTLKEQKKYVASVEAEFFKEYVALDAELMRFDQRNVDFKLLEPASPFSIDRNKPL
                     QLSWTANSDDPLIMELINWDGRIIYSSRGTVKSPYVIKGPLPAGIFVYRFRTSTQAYG
                     LGFLFLL"
     gene            3739..5868
                     /locus_tag="PKM34_04265"
     CDS             3739..5868
                     /locus_tag="PKM34_04265"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNQ82833.1"
                     /translation="MPLNRFSRYTPIRIAFPFMAGVALFFLTGEIFSVFTLLTLACLF
                     LAGLCIHYLFFRANFRWRWTAGVTTACCLAAFGNIVTCLHDPQHVAGSNNFGPRPDTS
                     ILLLRAGHTPEKKTRSYALKVKVVAMLTDTTAWERSGLKMLVYFTDSSCADIKYGDVI
                     ITRAVVQPISPPGNPNVFDFKKHMYNQGYTHQIFLKPHQWGKLNTSRSNPVRMYAEMC
                     RARFLDVLRKYRVEGEQFALVTALMIGERAYLEPETVREFSYAGAIHVLSVSGLHVGI
                     MYVLLDKLLFFLKRGKHGRKLQYILIIAGIWAYAVVTGLPSSVVRAALMFSIVAMANL
                     FRRDSDNFNSVATAAFLQLFINPFELTQIGFQLSYLAVLGIFAFYRPFNNMVVTGNKA
                     VAWIWSVIAVSLAAQLATGPLSVYYFNMFPVYFLITNLIVVPLASFITYFTVMLLFAG
                     AAGLYPEWLAWPLKWCLDLMLGSVEWIQSWPGAVLRPFLPDAATTIFSYAAILFLFIM
                     ISTRKKKWLLPLMSAIAAVSVLFAVNQYNRIERCALYVYQHKGSLAIDMMKNRRNLFV
                     TDGRMQKDSTRLQMLVEANRIKNRISLTDTLQISEKGQIGRQEYWYSRPFLNYMGKKI
                     VFIHDEWLQGNAGQRVQCDLAILSGQSGFNDPAVLNCVKFERAILAPDLSYSREERIV
                     KWLNDAGIPFHSVRKDGAFVMEWRSSE"
     gene            complement(5893..>6253)
                     /locus_tag="PKM34_04270"
     CDS             complement(5893..>6253)
                     /locus_tag="PKM34_04270"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82834.1"
                     /translation="RFNSDDFVQEQGMYIDDLEISINGIGIHEKTSDQPIDFRINPNP
                     ATDLLRIDYTLESAGSVEILIADLKGTTVSVLDNSYREKGDHFIHTSLEPLPAGSYMV
                     ILRHSGKQSSRKLIITE"
BASE COUNT         1673 a         1331 c         1490 g         1759 t
ORIGIN      
        1 caaacgggag agttgatagc cgattcggct accgtccttg gagtgaacgg tacacccact
       61 tttggattca acatcggcat cgtcggggat aaaagactcg gacgctattt taatgtccgt
      121 tttgtccctg cacttgtttt cggcgaacgt taccttaatt acagtgtgag agcctgggcg
      181 aacggcgagg aaaagctggt ggatataaag aagaatatcg gatcggtttt catcgatttt
      241 ccttttacca tcaaatacaa atccaaaagg cataataact atctcgccta cctcctggcc
      301 ggggggaaat acacgctcga cattgcttcc aatgccaaga aaaaggattc caacggacag
      361 gtggtggtca agctgaacaa gcatgatatc tatgctatcg tcggtgtagg ttttgatttt
      421 tacaatccct ggttcaagat gggagtggag ttaaagatgt cgtacgggtt actggatgtt
      481 ttaaaggatg ataaaacctt tttcacacag agcatcgata aaataaattc caaagtattc
      541 gaactgggag ttacctttga ataatcaaaa tcttatgtgc catgtcgcca ttacagaatg
      601 ccggacttta ccagattgtc aacaacaaag gcaagctcaa aaagagtgtt taccataata
      661 cgctcgaatc gcttaacctg atcaagaaga ctatcgggga gatgctggag gattaccggc
      721 gggaatttgc aaaagatcat gaggctatcc cattcgttgt caaagatatg ggtgaatttg
      781 agatccacct gcaatttgcc ggtgatgtcc tgatcttcaa catgcatacc aatgttttcg
      841 aattttcgag gtatcatgat gtgatgtcga cctcttacat ccgggaagac aaagcaaggt
      901 cgtactgtgg cattatcaat atttacaatt tcctggccga ttcatttaaa tacgaccggg
      961 ccaatgatat cggctatctt atcgggagga tttttatcaa taaggataag agctatttca
     1021 ttgagggcaa gcgggaagtc gggcttctgt acaataattt cggccaggag aagctggatg
     1081 agaccgctgt ccggaaggtc gtcgaatcgg ccgttttata caccattaat ttcgatttgc
     1141 tgacgcctcc gttcgattcc gtgaaagaaa tttccctttc ggagatgaaa tcgatcgccg
     1201 gcagccagac gcttaaaacc ggtaaacgat tgggtttcaa gtttcaggcc gaccgcgatt
     1261 aagaaaagta aagatttttt tcaaagtgtt ttgcaggata aaaaaatctt gctatttttg
     1321 caccctcaaa ttttggcccg ttcgtctagg tggttaggac gccaggtttt catcctggaa
     1381 atcaggggtt cgaatcccct acgggctacc agggccggtt tcatgccggc tttttttatt
     1441 tcgaccttat cctttccata aaattttacc ggtttcccct ttttttgttg atgattattt
     1501 atccgaattt tgtaaattgt cgttgaattt aaccccgacc aaaaatgccg gattgtatgg
     1561 aaaagattgt tgataatcaa tattatgacg cagccattga ggctaatatg gctgtcgttc
     1621 tgctaaaaac ccgcgccttt gacctgatca ccagcgtgcc cgacagcgat gaactgatgg
     1681 aatttatccg ggatgccgag cataatccaa aagtaaaagc gttgatgcta ctcaatgagc
     1741 ccgactgcct cggagagaaa gtgtatgatg aatttatccg gagcattttg aagagtgacc
     1801 aaaattccga aaatagcgac gtaccggcct tcctccagaa aaatacccgg ttccggcaga
     1861 ttatcaatct gaataagctg atccggtacc tggccggtta ccagaaactg gtcgtagtcg
     1921 gcgtgagcag caccattgtg actccattca tgggagtagc cctggtggcc gatttgcgcc
     1981 tggccagtcc aaaaggatgt tttgccctgg cccatacccg gtatggattg catcccagcg
     2041 gcgccatccc ctttttcctg acacattatc tcggccattc aaaagcactt gaagttcagc
     2101 tttccgaaag ggtttgcgct gaagaggctt ttcgcctggg tatcgttaat cagattcttc
     2161 cttccgaaga attttattcc aattgcatcc gttttgtcca gccgtatctg caacattgcc
     2221 gatccacgct ggcgatgaca aaaaggctta ataatttcaa aaaccgttcg ctgcaggaat
     2281 acctcgacca tgaagccagc ctgatgaacc tctgaattat aagttttttc ctgaaaaaag
     2341 gacaaaaact acctttatgc cgcctttgta atgtattgta aagcaatata ttaaaaaaaa
     2401 tttaaaaata ttttaaaacg accgggtatt tttttgtgaa aaaccggata aacagtatag
     2461 aatggtacac tataccaaat atgggattat agaaggaatc cggcgtaagg ataaagatgt
     2521 cctgcgatat gtctacaagc aatactttcc gatggtgagg tattttattg tcaggaataa
     2581 cggaacggag caggatgcgg aagacatatt ccaggaggca atgatcgtcg tctttgagcg
     2641 ggccagaaag agaaacctgg ccctgggttg cgcttttaag acctatcttt attcagtggt
     2701 caggcatatc tggctacaac tggcagaccg gaaccggttg cagtatgaac tgagcgatat
     2761 ggatgagttt cttttgctgg aagaaaaaga gatctatgac gatttccact ataagaaagt
     2821 catttttcag agaaaattcc ttgaattgac cgaattatgc aggaagctgc tgctgatggt
     2881 tgtcgaaggc aaaccctatg aagaaattgc cactattctg gggtataaag gcaaacaata
     2941 tgctgtgaaa cgcaaatatg aatgcatgaa atcattaatt aacagggtat tgaacgatcc
     3001 tgaatataaa aatctgacaa aatgaaaaca aaatatttaa aattgttcga aagatactac
     3061 aatggcgaaa tggatgagcc tgaaaaggaa atgtttcaaa gccaattgca gaacgaccat
     3121 gaaatggcgg atgcttacag ggaatacctg agtatttatg aagcattgaa ggacaaggaa
     3181 acactggaat tccggaccaa actgaatgaa atacgggatg aattcaggca taacaggaag
     3241 aaggaccggt ttatgaacga taaaaccaac tggcttttgg tggcggcact ttttatcctg
     3301 attgccggaa taaccgtcat tacctcattt ttcgtcctca ctctgaaaga acaaaagaaa
     3361 tatgtcgcct ctgtcgaagc ggaatttttt aaggaatatg tagccctcga tgcagagctg
     3421 atgagatttg atcagcgcaa tgtcgatttt aaactgctgg agccggcgag cccgttctcc
     3481 atcgaccgga ataaacccct gcaattgagc tggactgcta attctgatga tccgctcatc
     3541 atggagctga tcaactggga cggaaggatc atatattctt ccagggggac tgtgaaaagc
     3601 ccgtatgtga tcaaagggcc attgccggca ggtatttttg tttaccgatt caggacctcc
     3661 acacaggcat atggtttggg attcctgttt ttgctgtgag caggatgatg aggggcgctg
     3721 tgtcaaataa cggaatcatt gccattgaac aggttcagcc ggtacactcc gatcaggatt
     3781 gccttcccgt tcatggcagg cgttgccctg ttcttcctga ccggtgaaat cttttctgtt
     3841 tttactttat taaccctcgc gtgcctcttt ctggctggct tgtgtattca ttatcttttt
     3901 ttcagggcga attttcgctg gcgatggaca gcaggtgtta cgacggcctg ttgcttggct
     3961 gccttcggaa atattgtcac atgcctgcat gatccacagc atgttgcagg cagcaacaac
     4021 tttggaccca ggccggatac cagtatttta ttgctgagag ccgggcatac acctgaaaaa
     4081 aagacccggt cgtatgccct taaagttaag gtggtggcaa tgttgaccga tacaacggcc
     4141 tgggaaagat caggtttgaa aatgctggtc tatttcaccg acagcagctg tgccgacatt
     4201 aagtacggcg acgtaataat caccagagca gttgttcagc cgatttctcc accgggcaat
     4261 ccaaacgttt ttgattttaa aaaacatatg tataaccagg gatataccca tcagatcttt
     4321 ctcaagccgc atcaatgggg caagctgaat accagtcgct ctaatcctgt tagaatgtat
     4381 gcggagatgt gcagggccag atttctggat gttctgagaa aataccgggt ggagggtgag
     4441 caatttgcgc ttgttaccgc tttgatgatt ggggaaaggg cctatctaga acctgaaacg
     4501 gtcagggaat tcagttatgc cggcgctatt catgtgttaa gtgtttcagg gcttcatgtc
     4561 gggattatgt atgtcttgct cgacaaattg ctctttttcc tgaagcgtgg aaaacatggc
     4621 cgcaaactgc agtatatttt aattattgcc gggatatggg cctatgcagt agtgaccgga
     4681 cttccctcct ccgttgtcag ggctgcactg atgttcagta ttgtagctat ggcgaattta
     4741 ttccgcaggg attcggataa tttcaattcg gtggctactg ccgctttcct tcagctcttc
     4801 atcaatccct ttgaactaac ccagattgga ttccagttat cttacctggc tgtgctcggc
     4861 atttttgcat tttaccggcc tttcaataac atggtggtta ccggcaataa agccgttgcc
     4921 tggatatggt cggtcattgc cgtttcgttg gctgcccagc tggctacggg tccgctatcc
     4981 gtatattact ttaatatgtt ccccgtttat ttcctgatca ctaacctgat tgttgttccc
     5041 ctggcctctt ttattaccta ttttaccgtg atgcttttgt ttgccggagc agccggactt
     5101 taccccgaat ggttagcatg gccgttgaaa tggtgccttg acctcatgct gggttcagtg
     5161 gaatggatcc aaagctggcc cggcgccgtc ctccggccgt ttctgccgga tgcagccact
     5221 actatttttt cttatgctgc aatcctattc ctgtttatca tgatttcaac ccggaaaaaa
     5281 aagtggttat tgcctttgat gtcggcaatc gccgctgttt cggtcttgtt tgcagtcaac
     5341 caatacaacc ggattgaacg atgtgcactg tatgtgtatc agcacaaagg ctctctcgcc
     5401 attgatatga tgaaaaacag gcggaatttg tttgtcacgg atggaagaat gcaaaaggat
     5461 tcgacccgtt tacaaatgct ggttgaggcc aacaggataa aaaacagaat aagtctgaca
     5521 gatacactgc agatcagcga gaaagggcag ataggtcgtc aggagtactg gtattctcgt
     5581 ccttttctga attacatggg aaagaagatt gtttttatcc atgacgaatg gctgcaagga
     5641 aatgctggac aaagggtgca atgtgatctg gcgatcctat ccggtcaatc cggattcaat
     5701 gatccggctg ttttaaactg cgtgaagttc gaaagggcca tcctggctcc tgatctgtcc
     5761 tattcccggg aggagaggat agtcaaatgg ctcaacgatg ccggaattcc ttttcattct
     5821 gtcaggaaag acggcgcttt cgtgatggaa tggagaagca gtgaatagtg aataatagtt
     5881 gctaattgct gtttattcag ttattatcag ttttctggac gattgttttc cggagtggcg
     5941 caggatgacc atgtagctgc cggcgggaag aggctccagc gaggtgtgga tgaaatggtc
     6001 tcccttctcc ctgtagctgt tatctaaaac cgatactgtt gttcctttca gatcggcgat
     6061 caggatttcc acactgcctg ccgactcaag ggtgtagtcg atgcggagca agtctgtagc
     6121 aggattcgga ttaatccgga aatcaatagg ctgatcactg gttttctcat ggatcccgat
     6181 cccgttaatg ctgatctcaa ggtcatcgat atacattccc tgctcttgca cgaaatcgtc
     6241 gctattaaac cgg
//
LOCUS       DAOQXS010000105         6239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12877_length_6239_cov_3.571798, whole genome shotgun sequence.
ACCESSION   DAOQXS010000105 DAOQXS010000000
VERSION     DAOQXS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12877_length_6239_cov_3.571798"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PKM34_04275"
     CDS             complement(<1..1181)
                     /locus_tag="PKM34_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNQ82835.1"
                     /translation="MLDTGSTGFMLLACSLVMLMTPGLAFFYGGLATKRNILGIMIQS
                     FVSLGWTTVLWFIFGYSLCFSGGEGGIIGNLDKAFLNGVGIDSMYSNGKIPELVFVAY
                     QMMFAIITPALITGAFVNRVTFKAYLIFLTVWQILVYYPFVHMVWGGGLLAQMGVLDF
                     AGGIVVHATAGFAALASVFYIGARVDKQSVPNSIPLVAIGSGLLWFGWYGFNAGSEVA
                     VDHVTAIAFLNTDLAASFATIAWVVVEWIRERRPKFVGLLTGSIAGLATITPCAGFVP
                     VWAAPIIGTAAGLMCYVAVQWKNKMKWDDALDVWGVHGVGGFLGTILLGVFASKAVNS
                     AGTDGLIFGGGTFFWKQVLAVVAASAYAFIFTYIMLALINKISRVRVSEVEEKAGLDI
                     AL"
     gene            complement(1231..1989)
                     /locus_tag="PKM34_04280"
     CDS             complement(1231..1989)
                     /locus_tag="PKM34_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82836.1"
                     /translation="MKGSKKYWIILFAILAIPFRPLTAQEEKKLQPELGCDIVSSYIW
                     RGSAVYATLGDQNVLSPSLQPGFALGFQGLKLGAWGSVDFTGTYKELDYYLSYSLKGF
                     TLSVTDYYWASDWINDNFFEYGSDSTSHYLEASLAYKGEKIPVSILVATMLYGADKQF
                     DDPAKNNYSTYIEVGYGFKVSDYKLDLFLGMTPSDGMYGDGYGGRDGFAIVNAGVTGS
                     KTIKITENWSLPVKASLIANPQARKLFLAFILSI"
     gene            complement(2082..2732)
                     /locus_tag="PKM34_04285"
     CDS             complement(2082..2732)
                     /locus_tag="PKM34_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ82837.1"
                     /translation="MQKIKIILVDDHQIIRDGIRALFNGCTDIEVIAEAADAAECLQQ
                     LKLNQADVALIDISLPGISGIKLTEQLSVLYPSLKVLILSMHLNEAYISGAIKAGAMG
                     YLAKNTTREELISTIHLIAEGKKYLGKDVSEVITTGFVRRLQANENENTELLSKREME
                     ILKLTADGLGNREISQKLFISIRTVESHKNHIMQKLGLKSSVELVRYAIKSVLINI"
     gene            complement(2744..4894)
                     /locus_tag="PKM34_04290"
     CDS             complement(2744..4894)
                     /locus_tag="PKM34_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNQ82838.1"
                     /translation="MKKISIRERLILYFVLLGVISIAIVSLFSIFESKRGIRERAFSQ
                     LILLRDIRREQILNFYQVRKTELATLARTPVIRNLADRLEELQESGNFPVGLENDREF
                     LNLVLDNHICKVLYLVSVENNITKISGTDDIFLINKDEDLKVNESIMRLFEGSVTPDT
                     FHMTEWIGESNRPCLILTVPVFSSAGEVSALLLSRIEPEALNNIIFDVVPGTGMGKTG
                     EAYIAGPDGFMRTPSRFLPDAVMTVRINTEGFNKALTGIDSTGIYDDYRGIKVLGAYG
                     KIIFGGFTQLILAEIDVDEAMVPLAIIRNDILIVSSLILLGIFFITWFVAYGITRPII
                     RLKNAANVIASGSYNQKLKVGPEDEIGELTEAFNRMSEEVSIATRELREKEESLRHFY
                     DATLDGIVLHNDGKMVLFNAAILRLTGYTENELAALVIHDILKETKSLYCDKNQDNEI
                     YETLLFRKDNSSLPVEVQESCVEYKGNKIRASVIRDISTRKSMETELADERMKRLRAV
                     IDGKELEQQRLSRELHDGLGQQLAAGKLILESGLYTEGEKLKGKIVEAQAIFDQIIGD
                     IRRISHDLSPSVLKEFGLKEAVENLCASFRMTTGLRISFFCESDGYQPDEMKAIYLYR
                     IIQECLNNIQRHAGATLVSVFLKAGPQGIQLEIKDNGCGFNQKNVPASGGTGLYNIRE
                     RVNVMNGQLNIMTGKNKGTVIRIRIPLTDGGQNE"
     gene            5038..>6239
                     /locus_tag="PKM34_04295"
     CDS             5038..>6239
                     /locus_tag="PKM34_04295"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ82839.1"
                     /translation="MKRSLFLLIFAGLFFAGTNPEEKQVKIEYEKYKLANGLDVILHI
                     DRSDPIVALAVQYHVGSNRETEGRTGFAHLFEHMMFQRSENVPEDQFFKLIQDAGGML
                     NGGTSNDATTYFEVVPKNALEKILWMESDRMGFMINTVTKKSFAIQQNVVQNEKRQNY
                     DNRPYGFTQEVVAKNLYPEGHPYNWTVIGEMEDLFNASVEDVKAFHQRFYLPNNATVV
                     LAGDFDVDEAKKLIEKYFGEIPAGNEVKDPVPVPVKLDKTKKLYHEDNFAKAAQLRMI
                     WPTVEQFSDDSYALSYLGQLLSQGKKAPLYKVLEKEKKLTTSQNAYNSSQEIAGSFSI
                     NVTANDGVSLKDVEEAVFEAFRMFETEGFTDEDVERIKAGQETQFYNGISSVLGKSFQ
                     LAYYNEYAG"
BASE COUNT         1685 a         1547 c         1372 g         1635 t
ORIGIN      
        1 agggctatat ccaatccggc tttctcttca acttccgaga ccctcacacg agagatttta
       61 ttgatcaatg ccagcatgat ataggtgaag ataaaggcgt atgcagatgc tgcaacaaca
      121 gccaggacct gtttccagaa gaaagtgccg ccgccaaaga tcaacccgtc ggttccggcg
      181 ctgttcaccg ctttggaagc aaaaacgccg agcagtatcg ttcccaggaa cccacctaca
      241 ccgtgaacgc cccagacatc cagtgcatca tcccatttca tcttattctt ccattgaacg
      301 gcaacataac acatcagtcc ggctgcggtc ccaatgatcg gagcggccca aaccggcaca
      361 aaaccggcgc acggagtgat tgttgccagg ccggcgatgg aaccggtaag caacccgaca
      421 aacttcggac gacgctcccg gatccattca accacgaccc atgctatggt tgcaaaagat
      481 gcggccagat cggtatttaa gaacgctatt gctgtaacat gatcgactgc cacctcacta
      541 cccgcattga agccgtacca tccgaaccag agcaaaccgc tgccgatagc caccaggggt
      601 atactgttcg gaacagattg cttatcgacc ctggcgccga tgtagaatac cgaagcgagg
      661 gctgcaaaac cggctgtggc atgaacgaca atcccgcccg cgaaatcaag cacacccatc
      721 tgggccagca aacctccgcc ccaaaccata tggacaaaag gataatagac caatatttgc
      781 cagaccgtca gaaaaatcag gtaagctttg aaagtaaccc tgtttacgaa agctcctgta
      841 attaaagccg gtgtgatgat cgcaaacatc atctggtacg ccacgaaaac cagttcggga
      901 atcttcccgt tggaatacat ggagtctatg cccaccccgt ttaagaatgc tttatccagg
      961 ttcccgatta ttccgccttc tcctccgcta aagcaaaggg agtagccgaa aataaaccag
     1021 agaacagtcg tccatcctaa agaaacaaag ctctggatca tgatcccgag gatattcctt
     1081 tttgttgcaa ggcctccgta aaaaaatgca aggccggggg tcatcagcat gaccaggctg
     1141 caggccagca gcatgaaccc ggttgaacca gtatctaaca taattttaaa ttttgaatta
     1201 ttaattttga attataaatt aatgccttga ttatatagat aatatgaagg ccaagaataa
     1261 ttttcgagcc tgtggatttg caattaacga ggctttgacc ggcagtgacc agttttcggt
     1321 gatcttaatc gtttttgatc cggttacccc agcattaaca atggcaaagc catccctgcc
     1381 gccgtatccg tcgccgtaca tgccgtcgga cggggtcatc cccaaaaaca ggtccagttt
     1441 ataatcggat accttaaagc catatccgac ttcgatatac gttgaataat tatttttcgc
     1501 cgggtcgtcg aactgcttat cggccccata taacatggtt gccaccagaa tgctgacggg
     1561 aatcttttcg cccttgtaag ccagcgatgc ctccaggtag tgtgaagtgc tgtcgctgcc
     1621 atattcgaag aaattatcgt tgatccagtc tgaagcccag taataatcgg tcaccgacag
     1681 ggtaaatcct ttcaaagaat aactcaggta gtaatccagc tctttgtaag tacctgtaaa
     1741 atcgaccgat ccccaggccc cgagtttcaa tccctggaag cccaaggcaa atcccggctg
     1801 taaactcggg gaaagcacat tttgatcgcc tagtgttgca tatacggcag aaccccgcca
     1861 gatataactc gacacgatat cacaacccag ctcgggttgt agcttttttt cttcctgggc
     1921 tgtcaaaggc cggaagggta tcgccaggat ggcgaacaga ataatccaat acttttttga
     1981 acctttcata gttgtttttt ttaagaaagg tcaaattaaa gaattgaaga tcaaccggga
     2041 atcagaatta tcaccgaaat ctttaagtaa aacactgaat tttaaatatt gatgagcact
     2101 gatttaatcg catacctgac gagctcgacc gatgatttca gccccagttt ctgcatgata
     2161 tggttcttat gcgattcaac ggtccttata ctgatgaata atttttgaga gatttccctg
     2221 tttcccaatc catctgcggt caattttaag atttccattt ctctttttga aagcagttca
     2281 gtattttcat tttcattggc ctggagtcgc cggacaaaac cggtggtgat gacttccgaa
     2341 acatctttgc ccaggtattt tttgccttcg gcgatgagat gaatggtgga gatgagctct
     2401 tcacgcgtag tgtttttggc cagatacccc atggcgccgg cttttatggc gcctgaaatg
     2461 taagcctcat tcaggtgcat ggacaatata aggactttta atgatgggta caataccgat
     2521 agttgctcgg tgagtttaat gcctgaaatt cccggcaggc tgatgtcgat gagagccaca
     2581 tcagcctgat tgagcttgag ttgctgcaaa cactcagcgg catcagcggc ttccgctatc
     2641 acttcgatat cggtgcatcc gttgaataaa gcccggattc catcccggat gatctggtga
     2701 tcatcaacaa gaataatttt aattttctgc ataagaattt atgttattca ttttgtccac
     2761 cgtcggtcaa tggaatcctg atgcggatca ccgttccttt attttttccg gtcatgatat
     2821 ttaactgacc attcatgacg ttgaccctct cccggatatt gtacagcccg gttcctcccg
     2881 acgccggcac gttcttctga ttaaagccgc atccgttatc ctttatttca agctgaatac
     2941 cctgcggacc ggccttaagg aagactgata caagcgtagc ccctgcatgg cgctgtatgt
     3001 tgttcaggca ttcctggatg atcctgtaaa gatatatggc cttcatttca tccggttgat
     3061 aaccatcgga ttcacaaaaa aaactgatcc gcaaaccggt ggtcatccgg aaactggcac
     3121 agagattttc caccgcctct ttcagaccga attctttcag gacggaaggg ctgaggtcgt
     3181 gcgatatgcg ccttatatcg ccgatgatct ggtcaaagat ggcctgggct tccacgattt
     3241 tccctttcag cttttcccct tcggtgtaaa gtccgctttc cagtatcagc ttcccggccg
     3301 ccagttgctg gcccagcccg tcgtgcaatt ccctggaaag gcgctgctgc tccagctctt
     3361 tgccgtcaat taccgctctg agccttttca tccgttcatc ggccagttcg gtctccatgc
     3421 tttttctcgt gctgatgtcc ctgatcacgc tcgccctgat cttgtttccc ttgtattcga
     3481 cacaggattc ctgaacttca accggcaggc ttgaattgtc tttcctgaac aacagtgttt
     3541 cataaatttc attgtcctgg ttcttatcgc aatataatga tttggtctcc ttcagaatat
     3601 cgtgaatcac caatgccgcc agctcatttt cggtgtatcc ggtcaatcgc aggatcgctg
     3661 cattgaagag gaccatcttt ccatcattat gcaacacaat accatcgagg gtagcatcat
     3721 aaaagtggcg aaggctttct tctttttccc gtaattccct tgtcgcgata ctgacctctt
     3781 ccgacatcct attgaaagct tcggtcagtt ccccgatctc atcttccggg ccgaccttta
     3841 atttctgatt ataacttcct gatgctatta catttgcggc attttttaaa cgaatgatcg
     3901 gccgggttat accataagcg acaaaccagg taataaaaaa gatgcccaga aggatcaggg
     3961 aagagacgat caaaatatca ttccgaatga tcgccaatgg caccatggct tcatctacat
     4021 caatctcggc aaggatcaat tgggtgaatc ctccgaaaat aatctttcca taggctccta
     4081 aaacctttat tcccctgtaa tcatcataaa tacctgtact gtcaatcccg gttaaagcct
     4141 tgttgaatcc ttcggtatta atcctgacgg tcatgacggc atctggtaaa aagcgggaag
     4201 gggttcgcat aaaaccgtca gggcctgcaa tataagcctc cccggttttg cccatccccg
     4261 ttcccggaac cacatcgaaa atgatgttat ttaaggcttc aggttcaatc cgggataata
     4321 acaacgcgga tacctccccg gctgaactga ataccggaac cgtgaggatc aaacagggac
     4381 gattagattc tccaatccat tctgtcatgt gaaaagtatc cggtgtgacc gatccttcaa
     4441 ataaccgcat tatactctca ttgactttga gatcttcatc cttattaatc aggaatatat
     4501 catcagttcc ggatattttt gtgatgttgt tttcaactga aaccaaatac aaaactttgc
     4561 aaatatgatt atccagcacg aggttaagaa actcacggtc attctctaaa ccaacaggaa
     4621 agtttccgct ttcttgcaat tcttccaacc ggtctgcgag attcctgata acgggtgtcc
     4681 gggccagtgt agccagttcg gttttgcgga cctggtaaaa atttagaatt tgttctctgc
     4741 ggatatccct tagaaggatc aactggctga atgccctttc cctgatccct cttttggatt
     4801 caaaaatcga gaaaagactt acaatagcga tggatatgac acccagaagg acaaaataaa
     4861 gtatcagccg ttctctaatg gaaatcttct tcatggatca caaagaaaca aaatagtatg
     4921 gctggcatgt taaataaccg tttataatga tgtttttgca atttaacttt tgtcatttgc
     4981 aaattctcct atgcagttgt tatgcaattt ttttagtttt atcaaaaatt ttttaaaatg
     5041 aaaagatcat tatttctgct aatctttgcc ggactctttt ttgccggtac taatcccgaa
     5101 gaaaaacaag tgaaaatcga atacgaaaaa tacaagctgg ccaacgggct cgatgtgatc
     5161 cttcatatcg accggtccga tcctattgtc gcactggccg ttcagtacca tgtcggttcc
     5221 aaccgggaaa cggaaggccg caccggcttt gcccacctgt ttgaacatat gatgttccag
     5281 cgctcggaga atgtccccga agaccagttc ttcaaactga tccaggatgc aggcggaatg
     5341 ctgaacggcg gcacctcgaa cgatgccacg acctactttg aggtggtccc gaaaaatgcc
     5401 ctggagaaga tcctctggat ggaatccgac cggatgggat tcatgatcaa tacggtgacg
     5461 aaaaaatcgt ttgccatcca gcagaatgtc gtccagaatg aaaaacggca gaattacgac
     5521 aaccgcccat acggctttac ccaggaggtg gtggccaaaa acctttaccc ggaagggcat
     5581 ccgtacaact ggaccgtcat cggagaaatg gaagatcttt tcaacgcaag tgtagaagat
     5641 gtgaaagctt ttcatcagag attctatttg ccgaacaacg ctacggtggt cctggcggga
     5701 gattttgatg tggatgaagc gaaaaaactg attgaaaagt acttcgggga gatccctgcc
     5761 ggcaatgagg tgaaagaccc ggtcccggtt ccggtaaagc tggataagac gaaaaagctg
     5821 tatcatgaag acaactttgc caaagcggcg cagctccgga tgatctggcc aaccgttgag
     5881 cagttttccg acgactctta tgccctctct tacctgggcc agcttctttc gcaagggaaa
     5941 aaagccccgt tgtacaaagt ccttgagaaa gagaaaaagc ttactaccag ccagaatgct
     6001 tataacagca gccaggagat cgcggggagc ttcagcatca atgtgactgc caacgacggc
     6061 gtcagcctga aggatgtcga agaggccgtt tttgaggcgt tccggatgtt cgagaccgaa
     6121 gggtttaccg atgaggatgt ggagaggatc aaggcgggcc aggaaaccca gttctacaac
     6181 gggatcagca gcgtgctggg caaatcgttc cagctcgcat actacaacga atatgcagg
//
LOCUS       DAOQXS010000106         6193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_12983_length_6193_cov_3.774031, whole genome shotgun sequence.
ACCESSION   DAOQXS010000106 DAOQXS010000000
VERSION     DAOQXS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_6193_cov_3.774031"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKM34_04300"
     CDS             complement(<1..415)
                     /locus_tag="PKM34_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNQ82840.1"
                     /translation="MNLYEQLLDKQAKLSVIGLGYVGLPIALEFARKIKVIGFDLNAA
                     RVEMMKNRIDPSEELPSEAFDGCDIEYTADPARLRDAQFHIVAVPTPIDKHNLPDMTP
                     LLKATLTVGKALKKGDYVVYESTVYPGATEEDCIPI"
     gene            complement(506..1453)
                     /locus_tag="PKM34_04305"
     CDS             complement(506..1453)
                     /locus_tag="PKM34_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ82841.1"
                     /translation="MTQNFALIGAAGFIAPRHMKAIKDTGNNLVAAVDPFDCVGILDS
                     YFPNTNFFVETERFDRHLDKLRRKGENKVDYVSICSPNYLHDAHIRLALRNEAHAICE
                     KPLVLNPWNADALHEIEIETGRKINVILQLRYHPTIVELKKTIENGPKDKIYDVDLTY
                     ITSRGKWYFISWKGDMEKSGGVATNIGIHFFDMLSQIFGNTTENTVHYSDKFTAAGFL
                     QLERARVRWFLSVDYNTLPDDVRAKGQRTYRSIRIEGKEMEFSDGFTELHTESYRQIL
                     AGQGFGVGDARNSIETVYQIRNAAPAGKKGDYHPFLNRL"
     gene            complement(1672..2253)
                     /locus_tag="PKM34_04310"
     CDS             complement(1672..2253)
                     /locus_tag="PKM34_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNQ82842.1"
                     /translation="MNNRTDYFAHETAVIDDGCRIGAGSKIWHFSHIMAGCEIGEKCN
                     IGQNVVVSPGVKLGHNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIKNPRSAIIRKDQ
                     YATTLVRHGASIGANATIVCGITNGEFAFIGAGAVVVKDVPPYALVLGNPARHIGWMS
                     EFGHRLEFDENNIAVCPESGEKYRLEPGKVSKI"
     gene            complement(2416..3549)
                     /locus_tag="PKM34_04315"
     CDS             complement(2416..3549)
                     /locus_tag="PKM34_04315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ82843.1"
                     /translation="MRPIRMVDLKTQYDKIKPEVDAAIHEVIDTTSFINGPAVQRFQK
                     NLEQYLGIKHVIPCANGTDALQVSMMALDLKPGDEVITVSFTFVATVEVIALLKLTPI
                     LVDVEPGTFNMDIDALRKAITPKTRAIVPVHLFGQCANMEEIMDIAREHNLYVIEDAC
                     QAIGSDYTFSDGTIKKAGTIGHLGTTSFFPSKNLGCYGDGGAIFTDDDALATRLRFIV
                     NHGMHVRYYHEEIGVNSRLDSIQAAILDIKLKHLDEYAAARQKAAAAYNKAFGGNPHL
                     QVPVKSPQSTHVYHQYTLVTKGIARDMLLDFLLAKNIPCAIYYPVPMHMQKAYLDPRY
                     KEGDLPVTEHLSRTVISLPMHTELDEEQIEYITGTILEFIEKS"
     gene            3664..4425
                     /locus_tag="PKM34_04320"
     CDS             3664..4425
                     /locus_tag="PKM34_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82844.1"
                     /translation="MRFVSIAVLVIITQGLLAQVEVNEEDSALSIPMFYGFYGYQWPG
                     GDLDEQFGSNSVVGPGFLWKTKSNWLFGAEYDFHFGNNVKNTKEIFKNIYTTNGNVIS
                     GDGVPALVATFERCNSFSARFGKIIPVSENDRNSGIFFTVGAGYLSHKIKIEVENNSV
                     PQLRGDYKRGYDRLSGGLMLNQSVGFMYFGQNRLMNFTFSVEVVEAWVNPYRDYYFDL
                     MSPPPEGRQFDLLIGPKITWMLPLRQRNINKYYYY"
     gene            4438..5742
                     /locus_tag="PKM34_04325"
     CDS             4438..5742
                     /locus_tag="PKM34_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNQ82845.1"
                     /translation="MWFLYDLIIKFFGCAVCIAALFNKKARKWMQGRKDFSSKLTDFT
                     TVQLSNCPTVVWFHCASLGEFEQGRPVIEAFREEHPDWKIIVTFFSPSGYEIRKNYPG
                     ADHIFYLPIDTPRNARNFIDLINPSLVIFVKYEYWFRYLDELYRRNIPVYFISAIFRD
                     NQHFFRWYGGWALKQLKKITGFFVQDDASAQLLRRHGIDRVTVSGDTRFDRVAAIAEN
                     AKAYPEVGKFVSGSPVFLAGSTWPADEEMILHLIEEAGYRVKFIIAPHEVGRERVEAL
                     REKVIGRQSSVASRRIESGTMEQWNNGTILFSELNGENAAGAGVLIIDGIGYLSHLYR
                     YATIAFIGGGFGVGIHNILEAATFGKPVIFGPNYHKFREAEELVEEGGAFPVENAQQL
                     NNRTIELLTDPVLLQKTSAICSAYVMKKKGATGRILSEIKLS"
     gene            5836..>6193
                     /locus_tag="PKM34_04330"
     CDS             5836..>6193
                     /locus_tag="PKM34_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNQ82846.1"
                     /translation="MKKYPVPDDQEELFFSSLNNKTGYDKISALREGIPYYAFERYAV
                     SSPLSLADWSEFLEISERSLQRYKKDNKTFDRMRSERIMEIAQVLKKGEEVFGDKEKF
                     TIWMNSNIIALGGIKPR"
BASE COUNT         1485 a         1431 c         1600 g         1677 t
ORIGIN      
        1 ggataggaat gcagtcttct tcagtagctc ccgggtaaac ggtcgattca tagacgacgt
       61 agtccccttt tttcaatgcc ttgcccacgg taagcgtagc tttgagcagc ggcgtcatat
      121 cggggagatt gtgcttgtcg atcggggtag ggacagcaac aatgtgaaac tgagcgtcgc
      181 gcaaccgggc cggatcagcg gtatattcga tatcgcaacc atcaaaagct tctgatggaa
      241 gctcttcact gggatcgatg cggttcttca tcatttcaac acgcgcggcg ttcagatcaa
      301 agccgatgac cttgattttc ctggcaaatt cgagggcaat gggcagtccg acatagccca
      361 ggccgataac cgacagtttg gcttgtttgt caagcagttg ttcgtataga ttcataggta
      421 agtgtttgtt tcaattaatg atcttatttc cggattattt gtttcttaca gaggttcacc
      481 gagggtttca caaagggcac gaacgttaaa gcctgttcag aaatgggtga tagtcgcctt
      541 tcttacctgc aggtgcagca ttacggattt gatataccgt ttcgatgctg ttccgggcat
      601 ctccgacgcc aaagccctga cccgccagga tttgccggta gctctcggta tgcagttcgg
      661 taaaaccgtc gctgaactcc atctctttcc cttcgatccg gatggagcgg tatgtccgct
      721 gacctttggc ccttacatca tcaggaaggg tattgtaatc cacgctgagg aaccagcgga
      781 cacgggccct ttccagttgc aggaaaccag cggctgtgaa tttatccgag taatgaacgg
      841 tgttttctgt ggtgttgccg aatatctgcg aaagcatatc aaaaaagtgg atgccgatat
      901 tggtggccac tccgcccgat ttctccatat cccctttcca ggagataaaa taccatttac
      961 cacggctggt aatatatgtc agatcgacgt catagatctt atctttcggc ccgttctcaa
     1021 ttgttttttt tagttcaact atcgttggat gatatcgtaa ttgaaggatg acatttattt
     1081 tccggcctgt ttcaatttca atttcatgaa gggcatcggc attccaggga ttgagcacca
     1141 gcggcttttc gcagatagca tgcgcttcgt ttctcagtgc aagcctgatg tgggcgtcgt
     1201 gcaggtaatt cggcgagcag atcgatacat agtccacttt gttctcccct ttgcggcgca
     1261 gtttgtcgag atgccggtcg aatcgttccg tttccacgaa aaaattggta ttcggaaaat
     1321 aagaatccag aatcccgaca caatcgaacg gatctaccgc cgccaccagg ttgttcccgg
     1381 tatctttaat ggctttcata tgtcgtgggg cgatgaaacc cgctgctccg atgagtgcaa
     1441 aattctgggt catggatagt tggcgattat atttgtcatt aatcattagt cattagctcg
     1501 cttcgatcgc gtcaaaggct tactggcgtt cgcgtcatta gctcactgcg ctcgcgtcaa
     1561 aggcttactg acgtttgcgt cattggctca ctgcgctcgc gtcattaatt gtcattgtta
     1621 atctattatt agtgtttttt aaactttgaa ttttgaactt taaactttga actaaatctt
     1681 gctgactttt cctggctcaa gcctgtattt ctccccgctc tccgggcata cagcaatatt
     1741 attctcatca aattccagcc tgtggccgaa ttcgctcatc cagccgatat gacgggcagg
     1801 gtttcccagc accagcgcat agggcggaac atctttcacc acaaccgccc ctgccccgat
     1861 aaaggcaaac tcaccgttcg tgatgccgca tacgattgta gcattggccc cgatcgaggc
     1921 gccgtgcctg accagggtgg ttgcgtactg atctttccgt ataatcgcac tgcgcgggtt
     1981 tttgatgttg gtgaatacca tggagggacc gaggaaaaca tcgtcttcgc aaatcacgcc
     2041 ggtataaatc gagacgttat tctgcacctt cacattgtgt ccgagtttaa cacccggtga
     2101 gacgaccaca ttctggccga tattgcattt ttctccgatc tcgcaaccgg ccatgatatg
     2161 cgagaagtgc cagatcttcg atccggcgcc gatccggcag ccgtcatcaa taacggccgt
     2221 ttcatgtgcg aagtagtctg ttctattgtt caattgttca actgttaaat tgttaaactg
     2281 ttcaattgtt catccgttaa attgttaaac tgttaaattg ttgaactgtc agattgtttt
     2341 actgttttat tgctgttttg ttgtcctatt ctgctttttt cattcctaat gaccaacgac
     2401 gcgagcgcag cgagcttatg acttttcaat aaactcaagt attgtccctg taatatattc
     2461 aatctgttct tcatccaact ccgtatgcat cggcagcgag atcactgtgc ggctgagatg
     2521 ctcggtaaca ggcagatcgc cttctttata gcgtggatcc aggtaagcct tctgcatatg
     2581 catcggcacg ggataataga tcgcacaggg aatattcttc gccagcagga aatccagcag
     2641 catatcccgg gcgatgcctt ttgtgaccag cgtgtactgg tggtaaacgt gtgtggattg
     2701 cggcgacttc accggcacct gaaggtgcgg attgcccccg aaggctttat tgtaggcggc
     2761 ggcggctttc tgacgggcgg cggcatattc gtcgaggtgc ttcagcttaa tgtcgaggat
     2821 agcggcctgg atgctgtcga ggcgggagtt gacaccgatt tcttcgtggt agtaacggac
     2881 atgcatcccg tggttgacga taaaacgcag acgcgtagcc agcgcgtcgt catccgtgaa
     2941 gatcgcaccg ccgtcgccgt agcagcccag gtttttcgaa gggaagaacg acgtcgtacc
     3001 taaatgcccg atagtacccg cttttttgat ggtcccgtcg ctgaaggtgt aatccgaacc
     3061 gatcgcctgg caggcgtctt cgatcacata aagattgtgc tccctggcaa tgtccatgat
     3121 ctcttccata ttggcgcact ggccaaagag atgcacgggc acgatcgctc tggttttcgg
     3181 cgtgatggct ttgcgcagcg catcgatatc catattgaaa gtgccgggtt cgacatccac
     3241 caggataggc gtcagtttca gcagtgcaat cacctcaacg gtggcgacga aggtgaagga
     3301 gacagtgatg acctcgtcgc ccggtttcag gtcgagggcc atcatggaaa cctggagggc
     3361 gtcggttccg ttagcgcagg gtataacatg cttaatgcca aggtattgct ccaggttctt
     3421 ctggaaacgt tgcacggcag ggccgttaat aaaggaagtc gtgtcgatca cttcgtggat
     3481 ggctgcatcc acttcgggtt tgattttgtc gtactgggtt ttcaggtcca ccatccggat
     3541 aggtcgcata gagaggatat ttaatataag taaatgtttt aactggtgcg caaaggtagt
     3601 gaaaatggtg aacggtaagc ggtgaagggt gaagggaatt gttatctttg tgaaaaaata
     3661 cacatgcgct ttgtttcgat tgctgtactg gtcattatca ctcagggcct tctcgcccag
     3721 gtcgaggtaa atgaagaaga ttcagctttg agtatcccga tgttttatgg cttttacggc
     3781 taccagtggc ccggtggcga cctggatgaa caattcgggt caaactcggt ggtcgggccc
     3841 ggttttttat ggaaaacgaa atcgaactgg ctgttcgggg cagagtatga tttccatttc
     3901 gggaataatg tgaagaatac aaaagagata tttaagaata tttacaccac gaatggcaat
     3961 gtgataagcg gcgacggggt gccggctttg gtggcaacgt tcgagcggtg caacagtttc
     4021 agcgccaggt tcgggaagat catcccggta tcggaaaatg acaggaattc aggtattttc
     4081 ttcaccgtcg gcgcaggata tttatcacac aagatcaaaa tagaggtcga gaataacagt
     4141 gtcccgcaac tgcgcggtga ttataaaaga gggtacgacc ggctcagtgg cggtttgatg
     4201 ctgaaccagt cggtcgggtt catgtatttc ggccagaaca ggctgatgaa tttcactttt
     4261 tcagttgagg ttgtggaggc ctgggtgaat ccttatcgcg attattattt tgatctgatg
     4321 tcgcctcccc cggaaggaag gcagtttgac ctgctgatcg ggccgaagat cacctggatg
     4381 ctgccattgc gccagcgaaa catcaataaa tattattatt attaattctt tggcaggatg
     4441 tggttcctct acgacctgat cataaaattt ttcggttgtg cagtatgtat cgccgccttg
     4501 ttcaacaaaa aagcacggaa gtggatgcag ggcagaaaag atttttcttc taagctaacc
     4561 gattttacaa ccgtccaatt gtccaattgt ccaactgtcg tatggttcca ctgcgcatcg
     4621 ctgggcgagt tcgagcaggg gcggccggtg atcgaagcat tccgcgagga gcaccccgat
     4681 tggaaaatca tcgtgacatt cttttcgcct tcagggtacg agatcagaaa aaattatccg
     4741 ggagccgatc acatctttta tcttcctatc gatacgcccc gcaatgccag gaacttcatc
     4801 gacctgatca atccttcgct tgtcatcttt gttaaatacg agtactggtt ccgttacctg
     4861 gatgagcttt accgacgcaa catccctgtc tatttcattt cagccatttt ccgcgataat
     4921 cagcattttt tccggtggta tggaggatgg gcgctgaaac aactgaaaaa gatcaccggg
     4981 ttttttgtgc aggatgatgc ctctgcgcaa ttacttcgca ggcatggcat cgaccgggtt
     5041 acggtcagcg gcgacacccg gttcgaccgg gtagcagcga tcgcggagaa tgcgaaagcg
     5101 tatccggaag tcgggaaatt tgtgtcggga agcccggtgt tcctggcagg cagcacctgg
     5161 ccggccgatg aggagatgat ccttcacctt atcgaagagg cagggtacag ggtgaagttc
     5221 atcatcgcgc cgcacgaggt gggcagggag cgggtggagg cgctgagaga gaaagtcatc
     5281 ggtcgtcagt cgtcggtcgc cagtcggcga attgaatctg gaacaatgga acaatggaac
     5341 aatggaacaa tcctgttttc tgaactcaat ggtgaaaatg cagccggcgc cggtgtactc
     5401 atcatcgacg ggatcggtta tctttcccat ttgtaccggt acgccaccat agcatttatt
     5461 ggcgggggat ttggcgtggg tatccataat attctggagg ctgccacatt tggcaagccg
     5521 gtaatcttcg gcccgaatta ccataaattc cgcgaagcgg aagaacttgt tgaagaaggc
     5581 ggtgcatttc cggttgaaaa cgcgcaacaa ttgaacaaca gaacaattga actcttaacc
     5641 gatccggtac ttttacagaa aacttcggcg atttgttcag cctatgtcat gaaaaagaaa
     5701 ggggctaccg gcaggatcct ttctgagatc aaactttctt agcgcctctg cgcctccatg
     5761 tctccgcgat caagttaata cgacatatgt catcattatt cagacaaatg tcgtacattt
     5821 gtaaaaacta gttccatgaa aaagtacccg gttccggatg atcaggaaga attatttttt
     5881 tcttccttaa acaataaaac aggatatgat aaaatttctg ctttgcgtga aggcattccg
     5941 tactatgcat ttgaaagata tgccgtttca agccctttat cactggctga ctggtcggaa
     6001 ttcctggaaa tttccgaacg gagcctgcag cggtataaga aggataacaa gaccttcgat
     6061 cggatgcggt cggaaaggat catggaaatc gcccaggtat tgaagaaagg cgaggaggtg
     6121 ttcggtgata aggagaagtt cacgatatgg atgaacagca acattatcgc tctagggggt
     6181 atcaagcccc ggg
//
LOCUS       DAOQXS010000107         6151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13084_length_6151_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOQXS010000107 DAOQXS010000000
VERSION     DAOQXS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13084_length_6151_cov_3.666667"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PKM34_04335"
     CDS             complement(<1..1189)
                     /locus_tag="PKM34_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNQ82847.1"
                     /translation="MQKEERYSLDRFARNLNELAGKGKLDPVIGRDDEIRRVLNILSR
                     RTKNNPILIGEPGTGKTAIAEGLAHRIVKGDVPENLKSKQVYSLDMGALIAGAKYKGE
                     FEERLKGVVKEVVDSDGEIILFIDEIHTLVGAGGGEGAMDAANILKPALARGELRAVG
                     ATTLKEYQRFFEKDKALERRFQIVMVDEPDTQSAISILRGLKERYETHHQVRIKDEAI
                     ISAVELSQRYITDRYLPDKAIDLIDEAAARLRLEINSLPEELEVVDRKIRQLEIEREA
                     IKREKDQVKLGALTEEIKTLSSERERLMAQWRSEKEVVEKIQIRKKDIENFKFEAEKA
                     EREGNFGKVAELRYGRIREAEADLGRLKGQLDKIQAGSPMINEEVGSEEVADIVSKWT
                     GIPV"
     gene            1440..2480
                     /locus_tag="PKM34_04340"
     CDS             1440..2480
                     /locus_tag="PKM34_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNQ82848.1"
                     /translation="MKSYQSHAQFDCTIYPGDTTVCYGQSVDLYTEFADTMNYFWEPN
                     GETGVYLKVIVEDTMTYILHVYNDDSTFYCTDTVNLYIYPKVTIEYEQLVFGCPPADT
                     NSVDTICKAQIKANVTGGYPPYQYDWGDSVLVNYGDSSWALNLCVDQTYVLIVKDTVC
                     SYNLDLYVEPFDMPDLEVTVEPDSLFETNPQAILSFENKSADSIPLTSWTWIFPDGTS
                     TNQLTPSYVFTTKDSLVKFTYTTIDNCNDTIYIPILVKEFELSVPNVFTPNGDGINDK
                     YEIPYLDRYISNEILIFDRWGTLVYKEKNYNSGWDGGRLPDGVYFYILKCQGYWEEDV
                     FRGSVSIYGSRY"
     gene            2501..2956
                     /locus_tag="PKM34_04345"
     CDS             2501..2956
                     /locus_tag="PKM34_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ82849.1"
                     /translation="MRLINNLLILGIMAISACNGDPSRLPADVVNIPNSASGSVSENA
                     LPEMKFVKLEHDFGQVVMGEVVTYAFKFRNTGKGDLVIANISASCGCTASNFPKQPIK
                     PGEENFIEVSFNSEGRLGFQNKTLTVAANTQPGTIVLTIKAKVVSPELQ"
     gene            2976..3323
                     /gene="yajC"
                     /locus_tag="PKM34_04350"
     CDS             2976..3323
                     /gene="yajC"
                     /locus_tag="PKM34_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNQ82850.1"
                     /translation="MANLLYILLFAPPANPDGGSGSGSGSLLSFLPIILIILVFYLFF
                     IRPQMKKSKDQKKFREALKKGDKVITIGGLHGKILEVDETTVTLDVGNNMRLTFEKSA
                     IAMDTSSTIEQNK"
     gene            3369..4367
                     /locus_tag="PKM34_04355"
     CDS             3369..4367
                     /locus_tag="PKM34_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNQ82851.1"
                     /translation="MKISLLELFQSAFGKRDDKFRQRLSIFLVCVGISCIIWFTIKLS
                     EEYDTVVEYPVRYTHLPKNKSLTFTSDSILQVEIIEKGSNLFRMLYVDKIEPVTINLR
                     FLPIYPKGGSYHGIVTPSLLINEIEREKGLLGKIISISPDTIYLTFEPEKSRKVPVKA
                     DFDLTFDKEYMRYGNSVFKPDCVTVKGPERVIENLDSVSLGYIKLENLTDSFTGNKKF
                     INDSINRTLSISPSEVSFIVPVEKYTETQVMVPVKPVNANGKNVRLFPDKVKVYCTVA
                     LKDYNRIQEGMIKAQADMSKADQSGDDKIKVSLENFPPFIRVNRIEPEKVEYIIVR"
     gene            4367..4966
                     /gene="coaE"
                     /locus_tag="PKM34_04360"
     CDS             4367..4966
                     /gene="coaE"
                     /locus_tag="PKM34_04360"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNQ82852.1"
                     /translation="MLRIGLTGNMGSGKTFVADIFQSFGIPVFFADKEARKLFSHDKI
                     KIQIRGVFGNEVFDKEGNISRSQLAAIVFRNNHLLAKLNRIIHPAVIEEYKNWLEHYP
                     DVPYTLYEAAILYESGHYSDMDRIICVTAPEAVRISRVITRDGISPEEAKIRMENQWD
                     ESRKAEMADFLIVNDGIKEVKLQVEQIHQQIILLTKSAS"
     gene            4996..5529
                     /locus_tag="PKM34_04365"
     CDS             4996..5529
                     /locus_tag="PKM34_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82853.1"
                     /translation="MENLIFGIGSRVRHAKFGAGVVVQVWPDNYEIVFMDSGPKQIIH
                     GTEQLEVIDYRSPEPDQLSFDKVERILVKILRKFSDIQETVPLGDKWKGGKMIMQPAT
                     AGLQSKEVPIESFFHKIVMVRDRLRVLEQRVNASKLSDEEKIDIQQYITRIYGSLTTF
                     NVLFADKEDYFVGEKSV"
     gene            complement(5554..>6151)
                     /locus_tag="PKM34_04370"
     CDS             complement(5554..>6151)
                     /locus_tag="PKM34_04370"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNQ82854.1"
                     /translation="GPMEFFDKIPLKKDYRSQGVRVVPHAVARYGSFMEPGVILMPSY
                     VNIGAYIGEGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLEPVQAAPVIIEDDCFIG
                     SRCIVVEGVRVERESVLGAGVVLTSSTRIIDVSGNDAVEYKGMVPAGSVVIPGTYPKT
                     FPSGVFQVPCALIIGKRSASTDKKTSLNEALRNFQVAV"
BASE COUNT         1707 a         1503 c         1304 g         1637 t
ORIGIN      
        1 tgaccggtat tccggtccat tttgaaacga tatctgcaac ctcttctgaa cccacctctt
       61 cattgatcat gggactgccc gcctggatct tatccaactg gccctttagc cgtcccaggt
      121 cggcttcggc ttcccggatt cggccatatc ttaattccgc cactttgccg aaattacctt
      181 ccctttccgc tttctctgcc tcgaacttga aattctcaat gtcctttttc cggatctgta
      241 tcttctcaac aacctctttc tccgatctcc actgggccat caggcgttct ctttccgatg
      301 acagcgtttt tatctcttcc gtcaatgccc cgagtttcac ctggtctttc tcccgtttta
      361 tggcctcccg ctcaatctcc agttgcctga tcttccggtc gaccacttcc agctcttcgg
      421 gtaacgaatt aatttccagc cttagcctgg cggcagcttc atcgatcagg tcaatggcct
      481 tgtcaggcag gtaccggtca gtgatataac gctgcgatag ctcgaccgca gagatgattg
      541 cttcatcctt gatcctgacc tggtgatgag tttcataccg ctcctttaac cctctcagga
      601 tcgaaatggc cgattgggtg tcgggctcgt caaccatcac tatctggaac ctgcgttcca
      661 gggctttgtc tttttcaaag aaacgctggt attctttaag tgtcgtagca ccaacggctc
      721 tcaactcgcc cctggccaaa gccggcttga ggatattggc agcatccatg gctccttccc
      781 cacccccagc accgacaagt gtgtgaatct cgtcgataaa caggatgatc tcgccgtcgg
      841 aatcgacaac ctctttcacc acccccttca atctctcctc gaattcacct ttgtactttg
      901 ccccggctat caaagcaccc atgtcgagcg aataaacctg tttcgacttc aggttctccg
      961 gcacatcccc tttgacgatc cggtgagcca atccttccgc aatagccgtt ttacccgtcc
     1021 cgggctcacc gattaatatc ggattgtttt tggtccggcg cgacaaaata tttaaaaccc
     1081 gcctgatctc gtcatccctt ccgatgaccg gatccagttt acctttaccc gcaagttcat
     1141 tcaggttgcg ggcaaaccgg tcaagcgaat atctctcttc cttttgcata tcactccttt
     1201 ttgtaatttt cagccattga tcaacttcca taccacccca taaaagacta tccatgcgga
     1261 aattctgtca ggaaaccgtt ttatttactt ccatacaata aatatcctga ttgcacatta
     1321 ttgtttaagt aaggatgtgg tttcactata tagcaaaaat ccttactttt gcctcctgta
     1381 tgaaaaatac atcgatatac tcagggcttt ggtttagtat catgatgact atctttatca
     1441 tgaaaagcta tcaaagccat gcccagttcg attgtaccat ataccccggg gataccacgg
     1501 tttgctatgg tcagagcgtt gacctctata cggaatttgc cgatactatg aattacttct
     1561 gggagcccaa cggggaaaca ggtgtatacc tgaaggttat cgttgaagat accatgacct
     1621 atattctgca cgtctacaac gacgacagca cattctactg taccgatacg gtcaatcttt
     1681 atatttatcc taaggtcacg atcgaatacg aacaactggt cttcggatgc ccgccagccg
     1741 ataccaatag tgtcgatacg atatgtaagg cccagataaa agccaatgta acaggcggct
     1801 atccaccgta ccagtatgac tggggcgact ccgtactggt gaattatggc gattcttcat
     1861 gggccctgaa tctctgcgtt gatcagacct atgtcctgat cgtgaaagac acggtttgca
     1921 gctataacct ggacctctac gtggaacctt tcgacatgcc cgaccttgaa gtaacagtag
     1981 agcccgacag cctgttcgaa accaatccgc aggctatcct ctctttcgaa aataaatcgg
     2041 cggattcgat acccctgacc tcctggacct ggatatttcc agacggaaca tccacaaatc
     2101 agctgactcc cagctatgtt tttacgacga aggattcgct ggtcaaattt acctatacca
     2161 cgatcgacaa ttgcaacgat acaatttata tacccatcct ggtcaaggaa tttgaactga
     2221 gtgtgccgaa tgtctttaca ccgaacgggg atggaatcaa cgataaatat gaaatcccct
     2281 atcttgacag gtatatttca aatgaaatat taatctttga ccgatgggga acactggtat
     2341 acaaagaaaa gaactataac agcggatggg atggcggcag attgcccgac ggtgtttact
     2401 tttatatttt aaaatgtcag ggatactggg aggaagatgt cttcaggggt tcagtatcca
     2461 tttacgggag ccgatactga ctttttaagg ctgaaatatt atgcgactca ttaataattt
     2521 actcattctg ggaatcatgg ccatcagcgc ctgcaacgga gatccgtcac gtttgccggc
     2581 tgatgtggta aatattccta actcagcgtc tggttccgtc agcgaaaatg ccctgccgga
     2641 aatgaaattc gtcaaactgg aacatgattt tggtcaggtt gttatgggtg aagtagttac
     2701 gtatgctttt aaattcagga acacgggaaa aggcgacctt gtgatcgcca acatcagcgc
     2761 atcctgcggc tgtaccgcct ccaatttccc gaaacagccc atcaagcccg gggaagaaaa
     2821 ctttattgaa gtcagcttca acagcgaagg ccggctcgga tttcaaaaca aaactctgac
     2881 cgtcgctgcc aatacccagc ccggcactat tgtcctgaca atcaaagcaa aagtggtttc
     2941 acccgaatta caataacaat taatttttag aacatatggc aaatttactt tacatcttat
     3001 tattcgctcc tccagccaac ccggatggag gttccggctc cggctccggc agcctgcttt
     3061 cgtttttacc cattatcctg atcatcctgg tcttttatct tttctttatt cgtccgcaga
     3121 tgaaaaaaag caaggatcaa aagaaattcc gggaagcatt gaaaaaagga gataaggtga
     3181 tcactatcgg tggattgcac gggaaaatcc tggaagtgga cgagactacc gtcacactgg
     3241 acgtcggcaa caatatgcgc ctgacttttg aaaagtcagc tatagctatg gatacgtcat
     3301 caaccatcga acagaataag tagtccgtcg ttacgttttt acgataactc caggcaatct
     3361 ttaaagattt gaagataagt cttttagaac tttttcaatc ggcttttggg aaaagggatg
     3421 ataaattccg gcaacggtta tccatttttc tggtttgtgt cggcatctcc tgcattatct
     3481 ggtttacgat caaactgtcg gaggaatatg atacggtggt tgaatacccg gtccgttata
     3541 cccatcttcc taaaaataaa tcacttacgt tcacatccga ctcaatcctc caggttgaaa
     3601 taattgaaaa gggatccaac cttttcagaa tgctttatgt ggataaaata gagcctgtga
     3661 ctattaacct caggtttttg cctatatatc cgaaaggggg atcttaccac ggaattgtca
     3721 cgccatcgct gttaatcaat gaaatagaga gggaaaaagg gcttctgggt aagatcatct
     3781 ccatcagtcc cgacaccata tatctgactt ttgaacccga aaaatcaagg aaagtccctg
     3841 taaaagccga tttcgatctc accttcgaca aagagtacat gcgttacgga aactcggtct
     3901 ttaaacccga ttgcgtaaca gtaaaaggtc cggagcgtgt gattgaaaac ctcgattccg
     3961 tgtctttagg ttatatcaaa ctggaaaatc taaccgactc atttacagga aataagaaat
     4021 ttatcaatga tagtattaac cgcactctgt cgatcagccc atcggaagtc agctttatcg
     4081 taccggtaga aaaatatacc gagacgcagg taatggtgcc tgtgaaaccg gttaatgcga
     4141 atggaaagaa tgtcaggtta ttcccggata aagtaaaagt atattgcacc gttgcactta
     4201 aagattacaa cagaatccag gagggcatga tcaaggcaca ggcagatatg tcgaaagccg
     4261 atcagtcggg cgacgacaaa ataaaagtga gtctggaaaa tttcccgcct ttcatccggg
     4321 taaaccggat tgaacccgaa aaggtggaat atattattgt caggtaatgc tcaggatcgg
     4381 tcttaccgga aatatgggaa gtggaaagac gtttgtcgct gatatctttc aatcctttgg
     4441 cattccagtg ttctttgcgg ataaagaagc caggaaactt ttcagtcacg ataaaatcaa
     4501 aattcagatc aggggagtat ttggaaacga agtatttgat aaagaaggaa atatcagtag
     4561 atcgcagctg gctgcgatcg tttttaggaa caaccatctg ctggcaaaac ttaaccggat
     4621 tattcatccg gcggttatag aagaatataa aaactggctg gaacattatc ctgatgtacc
     4681 atatacctta tatgaagctg ccattcttta tgaaagcggt cactatagcg atatggacag
     4741 gatcatctgt gttaccgcac cggaagcagt aaggatcagc agggttatta cccgtgacgg
     4801 catcagcccg gaggaagcaa agatacggat ggaaaaccag tgggatgaat cccgtaaggc
     4861 agaaatggct gattttttaa tagttaatga cgggataaaa gaagttaaat tgcaagtcga
     4921 gcaaatacat caacaaatca tattgctcac aaaaagtgcc tcttagtgat caaccggcta
     4981 atttgaataa aaattatgga aaacttgata ttcggaatcg gatcgcgtgt gcggcatgcc
     5041 aaattcggcg ccggtgtggt cgttcaggtg tggcctgaca attatgagat cgttttcatg
     5101 gactcaggac caaaacaaat catccatgga acagaacaac tggaagtgat cgattaccga
     5161 agtcctgaac ccgaccagct aagtttcgac aaggtcgaac gaatattggt taaaattctt
     5221 cgcaaatttt ctgatataca ggaaactgtg cctctgggcg ataaatggaa aggcggcaaa
     5281 atgatcatgc agcctgcgac agctggtttg cagagcaaag aagtacctat cgaaagcttt
     5341 ttccataaaa ttgtgatggt tcgcgaccgg ctcagggttc tggaacaaag ggtgaacgcc
     5401 agtaaactat cggacgagga aaagattgat attcagcagt atattacccg aatctatggc
     5461 agtctgacca catttaatgt cttgtttgcg gataaagaag attatttcgt cggcgaaaaa
     5521 agtgtttgac tcagacaaag aaagaaaaac cgatcatact gcaacctgaa aattcctcag
     5581 cgcttcattc agcgatgttt tcttatcggt agaagcactc cgttttccga tgatcaatgc
     5641 acaaggcacc tggaatacgc cagaagggaa tgtcttggga taggttcccg gaatgacgac
     5701 gctccctgcc ggcaccatcc ctttatattc caccgcatca ttccctgaaa catcgatgat
     5761 ccgggtcgaa gaggtcagca caaccccggc tcccagcacc gactcccttt ccaccctcac
     5821 cccttcaacc acaatacacc tggaaccaat gaaacaatca tcttcgatta tcaccggcgc
     5881 tgcctggacc ggttcaagca ccccccctat gcctacacca ccgcttaaat ggacattccg
     5941 ccctatctgt gcgcatgacc caaccgttgc ccaggtgtct accattgtgc cttcgccgat
     6001 atatgcgccg atatttacat aagacggcat caggatcaca cccggttcca tgaaagaccc
     6061 gtaccgggct accgcgtgcg gtacaaccct tacaccctgg ctacggtaat ctttcttcaa
     6121 tggaatttta tcgaaaaact ccatcggccc c
//
LOCUS       DAOQXS010000108         6118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13176_length_6118_cov_4.013360, whole genome shotgun sequence.
ACCESSION   DAOQXS010000108 DAOQXS010000000
VERSION     DAOQXS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13176_length_6118_cov_4.013360"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKM34_04375"
     CDS             complement(<1..602)
                     /locus_tag="PKM34_04375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNQ82855.1"
                     /translation="MNIFLRIFTIPRAACHLRSLFLNLRLGFRDRFRIVVYRRTKVCI
                     SRKGKLIFDPGARLRLGLCWPMTNPNFSTLKIDEHAEMVVHGDWIFHSGIFISVNKGA
                     RLELGSGISNNDADITCFNSIRIGNHVFISKGVIIRDSDNHNVLKEGYLQSKPILIGD
                     NVWIGMRAIILKGVTIGDGAVVAAGAVVTRDVPPNTVVGGV"
     gene            complement(639..1556)
                     /locus_tag="PKM34_04380"
     CDS             complement(639..1556)
                     /locus_tag="PKM34_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82856.1"
                     /translation="MKLGITVVYFVKKDEGWLIDLHLNYIRKNTRVPYKIYAGAERLL
                     KEYRGKLEDSGDLIICDLSTTKDIAGKENSFYLEQLFRIAIDDGCTHIATFHVDSFPL
                     GSGWADSMLNKLDQGFQLAAIERLEDNDHKPHSSFMFFSSEYYLKYHPPLRLEEEEKN
                     TDTYKNYSKRFPHIQDSGYGFGYRLYRDNLTWYPLRRSNDKQNGRLMAGVYDDIVFHL
                     GGAGFTFHISRNDKIWYWRREAVLRLLFRFMRKSGIPTGKLETRYLPGFDHSKTKTRI
                     NNRNNTIFRKVMEKLKKDPDRFLDSLRMK"
     gene            complement(1553..2836)
                     /locus_tag="PKM34_04385"
     CDS             complement(1553..2836)
                     /locus_tag="PKM34_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82857.1"
                     /translation="MIEELKHLDHPIRLGIVGMGAMGRGLLYQAGVTPGFRYVAVNDI
                     VPRRAEECARWLGIEYINTGRSQKPEEVIASGKLAVAADPLDVARCNQVDVFIEASSG
                     IVESALYCKTAIEHGKHVVMMNAEADLAFGPCLMKLAMEHGVVYSSCDGDQPGIIKHL
                     YDRMVFWGFEPVMAGNIKGFLDRYSDPVKIIPEADKRRLDYKMATAYTDGTKLNIEMS
                     LVANALNMRTKIPGMYGPKAKDIREVFGLFDFEKLWADRIPFVDYILGAEPGGGVFTV
                     GYCEDDYQQFMMNYYKMGDGPFYLFYRPYHLCHVESMEWIARAYLYKEALLKPDFGFK
                     TNVIAYAKKDMKKGEMLDGLGGFACYGQIENTGDYAEEGLPVCLAEKVVLQRDMLRDE
                     RIMMNDVDPAGNEMGFELHRQSVKACQEVLFPAIL"
     gene            2933..4912
                     /gene="gatE"
                     /locus_tag="PKM34_04390"
     CDS             2933..4912
                     /gene="gatE"
                     /locus_tag="PKM34_04390"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNQ82858.1"
                     /translation="MAIKQKFDPKSNYALTLEMIGYVPRKQATQADYDRIGFMSGLEV
                     HQQLLTKYKLFCRCPAGVYNKHEVYDAELIRHMRPTLSELGEYDGTALMEFKTKKEIV
                     YRIKNETACTYDVDDTPPFKIDQEALNIAIEISLLSKLNIVGEVHITRKQYLDGSIPT
                     GFQRTAIIGVEGEIPLKFKKIRLIQLSLEEDSCREMWDVGHQRGYKTDRLGMPLIETV
                     TYPDCTNPEEVREACNYIRFLNRSTGKVRTGMGAGREDVNVSCRGGSRVEIKGVAHTR
                     WIPELTHNEAFRQWALLHIRETLKQRVKNPGNWKIASKILAYHNFNFKYKPLREARNS
                     KNRIVAVNLPDFKGLMSHFTQPGKIFADEISDRLKVIACIEKPHMAHSEDLTPLVTLK
                     DWETIRKMFKAKENDAQLIFWGPEADMTTALDVIEERCRMAFEGVPNETRKSFEDGTT
                     IFERVLPGADRMYPDTDSAPIPLEDELIEELRKNQPADVIDRYHQLVAWKVPEDTYTY
                     IFSKNLFPVIEKIIKDLKINPRLVGTFFGHHLKWVEGQHVKSAEFSYEMIFALFRYLK
                     ENKLDLILAGNMLPVIYLHPKMDFESVLTSIGFKKVKKEDILAKAEFLNEKFKQIGKK
                     QSPITRHNWVMGELRKVALGNIDLTELSKEIKHFV"
     gene            4955..>6118
                     /gene="gatD"
                     /locus_tag="PKM34_04395"
     CDS             4955..>6118
                     /gene="gatD"
                     /locus_tag="PKM34_04395"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNQ82859.1"
                     /translation="MTDDIFQGYKGDALATLKKFNTRVWGQTVVETTRGTFTGTVLPR
                     AENDDDKHIVVKIPTGYNIGVDVRTITSMKETGYKKAVYKIPEKEFPITPGLPKVKLL
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLETEKIFAVFSENMGPKQYV
                     ALSKAIGKEIENGIDGIVIGHGTDTLHHTGAVLTFMCQNLPVPVVLVGSQRSSDRPSS
                     DAALNLMHAMTAAGTGDIAEVMVCMFGPTSDEYGLLHKGTRVRKMHSSYRSTFRTIGD
                     VPVARVSRKGVIPIHKNYNKRRNDRNVNIIPYFSDEVTMLYYYPGIKPDVMDAMIDAG
                     YRGIIIVGTGLGHVNKELYPALVRAKEKGVHVFMTVQTIWGYVHMFVYDTGRDM"
BASE COUNT         1629 a         1619 c         1447 g         1423 t
ORIGIN      
        1 acgccaccta caacggtgtt cggaggcacg tcccttgtca caacggctcc ggcagcgaca
       61 acggcgccat caccgatcgt tactcctttt aagataatcg cccgcatccc gatccagaca
      121 ttgtcgccga taaggatcgg tttcgattgc aaataccctt ctttcagtac gttatgatta
      181 tccgaatccc ggataatcac ccctttggaa ataaaaacat gatttccgat cctgatgctg
      241 ttaaaacaag ttatatcggc atcattattc gaaattccgc taccaagttc cagccgggct
      301 cctttattca cagagatgaa aatcccggaa tggaatatcc agtcgccatg cacaaccatt
      361 tcagcatgct catcgatctt aagggtcgag aagttcggat tggtcatcgg ccagcacagg
      421 cctaacctga gcctggctcc cgggtcaaag atcagtttgc ccttgcgtga tatgcaaact
      481 ttagttctcc ggtagaccac gatccggaac cggtcccgga atccgagccg caggttcaga
      541 aagagggatc tgaggtggca cgccgcccgg ggaattgtga atatccgtaa gaatatattc
      601 atgttatgct tcttttgcga atattttatt tcctgttatc atttcattct cagggaatca
      661 agaaaacgat ccgggtcttt tttcagcttt tccatgactt tcctgaagat ggtgttgttt
      721 cggttgttta tccttgtttt tgttttggaa tgatcaaacc cgggcagata acgggtttcc
      781 agcttgcctg tcgggatacc tgattttcgc atgaaccgga acaataatct cagcacagcc
      841 tcccggcgcc aataccagat cttatcattt cttgaaatat ggaaagtaaa tcctgcgcca
      901 ccaagatgaa agacgatgtc gtcataaacg ccggccatta accggccatt ctgcttgtcg
      961 ttgctgcgcc gcaaaggata ccacgtcagg ttatctctat aaagccggta accgaaacca
     1021 tatcccgaat cctgaatatg cggaaatctc ttgctgtaat ttttatacgt atccgtattc
     1081 ttctcttctt cttccagtct caggggagga tgatatttca aataatattc cgaagaaaaa
     1141 aacatgaacg aagagtgggg tttgtgatca ttgtcttcca gccgttcaat agctgccagt
     1201 tgaaatccct ggtccagctt attcaacatg ctgtccgccc atccgcttcc caaagggaat
     1261 gaatcaacat gaaatgtggc aatatgcgtg cacccgtcgt cgatagcgat gcgaaataat
     1321 tgttccaggt aaaaactgtt ttcctttccg gcaatatctt ttgtcgtact gagatcacaa
     1381 atgatgagat caccggaatc ttcgagcttg cccctgtact ctttcagcag cctttcagcc
     1441 ccggcataga ttttatacgg aacccgggta tttttgcgga tgtaattaag atgaagatcg
     1501 ataagccatc cctcatcctt cttaacgaaa tatacgacgg taatcccaag tttcataata
     1561 tagctggaaa tagtacctcc tggcatgcct taaccgactg ccggtgcaat tcaaatccca
     1621 tttcatttcc tgccgggtca acatcattca tcataatccg ctcatctcta agcatatcac
     1681 gctgcaaaac aactttctcc gccaggcaaa ccggcaatcc ttcttccgca taatcgccgg
     1741 tattctcgat ctgcccgtag caggcaaatc cgcccaggcc atccagcatc tccccttttt
     1801 tcatatcttt cttggcatag gcaatcacgt tcgttttaaa tccaaaatcg ggtttgagca
     1861 acgcttcttt gtaaagataa gcccgggcga tccactccat cgactctaca tggcaaaggt
     1921 gataaggccg gtagaaaagg tagaacgggc catctcccat tttataataa ttcatcatga
     1981 actgctggta gtcgtcctca cagtaaccca ccgtaaaaac cccgccaccc ggctcggcgc
     2041 ccaggatata atcaacaaac ggaatccggt cggcccacaa tttttcaaaa tcaaacaggc
     2101 cgaagacctc ccggatatct tttgccttcg gtccgtacat tcccgggatc ttcgtcctca
     2161 tattcagcgc gttggccacc agcgacatct caatattcag cttcgttcca tctgtgtagg
     2221 cggtcgccat tttgtagtca agccgccgtt tatccgcttc gggaataatt ttaaccggat
     2281 cggagtagcg gtcgagaaaa cctttgatgt tgccggccat gaccggctca aaaccccaga
     2341 agaccatcct gtcgtaaaga tgcttgatga tcccgggctg atcgccgtcg caactggagt
     2401 aaacaacgcc atgttccatg gcaagcttca tcaggcaggg gccaaaagcc aggtcggcct
     2461 ccgcattcat catgacaaca tgcttaccgt gttcaatcgc cgttttgcag tataaagccg
     2521 attccacgat tcctgaagag gcttcgatga atacatcgac ctgattgcac cgggcaacat
     2581 ccagaggatc agcggccaca gccagtttac cagaagcaat gacctcttcc ggtttttgag
     2641 accggccggt attgatgtac tctataccaa gccaacgggc acattcttcc gcacgtcgcg
     2701 gaacgatgtc gttaacggcc acatatcgga atcccggtgt aacccctgcc tggtaaagca
     2761 ggcccctgcc catggcaccc atcccgacta ttccaagcct tatgggatga tcgaggtgtt
     2821 ttaattcttc aatcatacag caagactatt ttcagctaaa ttagaaaata gttgatacaa
     2881 ccatgagtgc aaatttcctt acttttgacc ccttgttatt tgattcacaa ttatggcgat
     2941 taaacagaaa ttcgatccta aatccaacta cgccctgacc ctggaaatga ttggctatgt
     3001 tccacggaaa caggctaccc aggccgatta cgaccgtatc ggcttcatgt cggggctgga
     3061 ggtgcaccag caactgctaa caaaatacaa actgttttgc cgctgccctg ccggtgttta
     3121 caacaagcac gaagtttacg acgccgaact gatccgccac atgaggccca cgctgagtga
     3181 attgggcgaa tacgacggaa cggccctgat ggagttcaag acgaaaaaag agatcgtcta
     3241 ccggatcaag aatgaaacgg cctgcaccta tgatgtcgac gacacgccac cattcaagat
     3301 cgaccaggag gcgctgaata tcgccatcga aatttcgctc ctctcaaagc tgaatattgt
     3361 cggtgaagtg catatcacac gcaagcaata cctcgacgga tcgatcccga caggattcca
     3421 gcgtacggcc atcatcggcg tggaagggga aattccgctg aagttcaaga aaatcaggct
     3481 gatccagctc agcctcgaag aagattcatg ccgtgagatg tgggacgtcg gccatcagcg
     3541 cggttataag accgaccggc tcggcatgcc gctcatcgaa accgtgacct accccgactg
     3601 cacaaacccg gaagaggtca gggaagcctg caactatatc cgtttcctga accggagcac
     3661 cggcaaagtg cgcaccggga tgggagccgg ccgcgaagac gtcaatgtga gctgccgcgg
     3721 cggttcccgg gtagagatca aaggcgtggc acatacccgc tggataccgg aactgacgca
     3781 caacgaagca ttccgccagt gggcgttgct ccacatccgc gaaacgttga aacagagggt
     3841 gaaaaatccg ggcaactgga agatcgcttc caaaatcctg gcctatcaca atttcaattt
     3901 caagtataag ccgctacgag aggcccgcaa cagcaaaaac aggatcgtgg ccgtcaacct
     3961 gcccgatttc aaaggcctga tgtcgcattt cacccagccc ggcaaaatat ttgccgacga
     4021 gatcagcgac cggctcaagg tgatcgcctg catcgaaaaa ccccatatgg ctcattccga
     4081 agatcttacg ccactggtca ctttgaaaga ctgggaaacg atccggaaaa tgttcaaggc
     4141 aaaggaaaac gatgcccagt tgatcttctg ggggcctgaa gccgatatga cgacagccct
     4201 cgatgtgatc gaagagcgct gcaggatggc ctttgaagga gtcccgaacg aaacgcgcaa
     4261 atcgttcgaa gatggcacaa ccatcttcga aagagtacta cccggcgccg accggatgta
     4321 ccccgacacc gactcggcac cgatcccgct ggaggatgaa ctgatcgagg aactgcggaa
     4381 aaaccagcct gccgatgtca tcgaccggta tcaccagctg gtggcctgga aagtgcctga
     4441 agatacatac acctacattt tttccaaaaa cctgtttccg gtcatcgaaa agatcatcaa
     4501 agacctgaag atcaatccga ggctcgtcgg gactttcttc gggcatcacc tgaaatgggt
     4561 cgaagggcag catgtgaaat cggccgaatt cagctatgaa atgatctttg cccttttccg
     4621 ttacctcaaa gaaaacaaac tggacctgat cctggcggga aacatgctgc cggtgatcta
     4681 ccttcatccg aagatggatt tcgagtcggt gctgaccagt atcgggttta aaaaggtcaa
     4741 aaaagaagat atcctggcca aagccgaatt cctgaacgag aaattcaaac agatcgggaa
     4801 gaaacaatcg cctattacac gccacaactg ggtgatgggt gaactaagga aagtggcatt
     4861 ggggaatatc gatctgacgg agttaagtaa agagattaaa cattttgttt aacgcatttt
     4921 gttaaatgat aatattttct tacagaataa atatatgact gacgatatct tccagggcta
     4981 taagggcgat gcgctcgcca cactgaaaaa attcaacacc cgggtctggg ggcagacggt
     5041 ggtggagacc acccgcggga cctttacagg cactgtattg ccgcgggctg agaatgacga
     5101 cgacaaacat atcgtggtaa agattccgac cggttacaac atcggcgtcg atgtgcggac
     5161 gatcacctcc atgaaggaga ccggctataa aaaagctgtt tacaagattc ctgagaaaga
     5221 atttccgata acgccaggat taccgaaggt aaaattgctc ggaaccggcg gaacgattgc
     5281 ttcgcgtctg gattatcgca ccggcgccgt aatccctgct ttttcgccgg gtgagctcta
     5341 cggagccgtg cccgaactgg ccgacatctg caacctggag acggagaaaa tatttgccgt
     5401 tttcagcgaa aacatgggcc cgaagcagta tgtagccctt tcaaaggcta tcgggaaaga
     5461 gattgagaat gggatcgacg ggatcgtgat cgggcatggg accgatacgc tgcatcatac
     5521 cggcgcagtg ctcaccttca tgtgccagaa cctgcctgtc cctgtcgtcc tggtcggctc
     5581 acaacgttcg agcgaccggc ccagcagtga cgccgcgctc aacctgatgc atgccatgac
     5641 ggccgccggc acaggcgata tcgccgaagt gatggtctgc atgttcggcc cgacatcgga
     5701 tgagtacggg ttgttgcaca aaggcacccg ggtgagaaaa atgcattctt cctaccgttc
     5761 taccttccgg acgatcggcg atgtacccgt agcccgtgtc agccgcaaag gcgttatccc
     5821 catccacaaa aattacaaca aacggcgcaa cgaccgcaat gtgaatatta ttccctactt
     5881 cagcgatgag gtaactatgc tctattacta tccgggtatt aaacccgatg tgatggatgc
     5941 catgatcgac gcggggtaca gggggatcat catcgtaggt accggcctcg ggcatgttaa
     6001 caaagagctc tacccggcac tggtcagagc gaaagaaaaa ggggtccatg tcttcatgac
     6061 tgtccagacc atctggggct acgtgcacat gtttgtttat gacaccgggc gcgacatg
//
LOCUS       DAOQXS010000109         6028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13419_length_6028_cov_3.322618, whole genome shotgun sequence.
ACCESSION   DAOQXS010000109 DAOQXS010000000
VERSION     DAOQXS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13419_length_6028_cov_3.322618"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="PKM34_04400"
     CDS             complement(<1..1835)
                     /locus_tag="PKM34_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ82860.1"
                     /translation="MMKYLHTFVFSLILVMILSSCTESVNKEPLIGKKELKLATDRMT
                     PEVLWSFGRVGEFEVSPDLQKILFSITYYDIDRNKSNRELYLIGTDGKNLKQITKTPE
                     NEFNYTWRPDGQKILFAGTASGSVQVWEMNPDGSGRKQLTDIPGGISGFKFSADMKKI
                     AYSAEVNLDDQYKKDLFEGLPLTSGKVYHSMMFRHWDEWRDTYSHLFIADFQDGKLSN
                     VTDILEGEPFDFPNKPFGGMEQMSWHPDGKKLAYSCHKNFGKAYAVSTNTDIFIYDLE
                     TRQQTLLTRDMKGYDNDPVFSPDGRHIAWTSMERDGYEADKSRLMVFDLESNSRVDYT
                     GIFDNDAEGPVWADDSQSIYFVAYWYGSKEIFNLHLNGTITQLSSGVHDFTAAQPAGD
                     KIIASMTTMSMPAELFAVPAAGGKETQITFTNKDILDQLSLGKVEKRFVNTTDGKKML
                     TWVIYPPHFDERNKYPALLYCQGGPQNALSQFWSYRWNFQMMAANDYIVVAPNRRGVP
                     GFGQAWKEQISGDYGGQNMQDLLAAIDDVSNEGYVDKDRLGAVGASYGGFSVYWLAGH
                     HERRFKAFIAHDGMFNIESAYLETEEQWFPNFDLGGAPWEKDNPVV"
     gene            complement(1872..4016)
                     /locus_tag="PKM34_04405"
     CDS             complement(1872..4016)
                     /locus_tag="PKM34_04405"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNQ82861.1"
                     /translation="MIRKLTTTGLGACISVFLSAQNLLPADGYLIINAGPGDPVYTTY
                     TAAPARSSLYDDKGYTMIYDEFSSPLTYGSEHGGRFFTVWEINSVVVSRVGDFHVKPF
                     IKATFPDMALMEYEPWPGISAEEIFFVYNSNCAVVDCHLKNNTPNTHEINIYPVLEDA
                     DSLYVIGFDSTSNSYCFNHYETKKRLISNLSDKYPYPDHFYNLFSAGFPIYSYGAYQG
                     NREDLYNAIKTDFYIENRSDSLNMADSSDAGFIALHGKYILQPGATIHYRYCRLRQSR
                     DKEPERLGKILEDAKSLSIQPFIDENVRLFSNIPLIDFSDPADKMVYLSAFNLARGCF
                     LPAEGQTTHNYYVFSREPLWGWGHGHQVLHESLSMLAYAYLDPVSAQNSQRVYMEQQS
                     EDGLIAYRHGPRGPQTYPHKEMPTTSAPFYSWINLEIYNVSHDREFLADAYESGTRYA
                     RWLINNRDTDHDGTFEWGPYGIIENVRDWYNAVFQVSKERYLDIDKEDISDELECLDL
                     SLMMVKEMRSLAAMATALDLREESEEWESKANKLARLINERMWDDASGFYYSVDKTDH
                     SFYFMTRDLKRQEIIGFLPLWTQVADSTRAVRLVQTLTDPGKFWRKYGVPTLAADDEW
                     YSPYVDYCCKWNGPVWLLWDYMVYEGLRNYGYDSLAEELAVNLLGAVKVQLSRNHRFP
                     ESYSPDNEVLNCPPNYIWDSIIARLMIRMNSD"
     gene            complement(4024..4260)
                     /locus_tag="PKM34_04410"
     CDS             complement(4024..4260)
                     /locus_tag="PKM34_04410"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNQ82862.1"
                     /translation="MSEQESLKNVFTGSVLEANYIKSLLEESGIGAMVRNTMGESLVA
                     GWVSGGPEDAGLVYVIESHEEEARKIVEEYLAGK"
     gene            complement(4316..5242)
                     /locus_tag="PKM34_04415"
     CDS             complement(4316..5242)
                     /locus_tag="PKM34_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ82863.1"
                     /translation="MPGRKAFIIGATAISFAAIMWGLDGVVLTPRLYNLDVGLVVFIL
                     HLIPFLLMQLFLFREYRHLKTFRRNDYILLLLIALFGGAIGTLSIVKALFLINFKELS
                     IVVLLQKLQPVFAILLATLLLKERINRYFILWASVALLGGYFMTFGFKIPDVHTGSNT
                     ALSALFALLAAFSFGSSTVFSKMILRRYSFSTATFYRYGFTTVIMLLYVIITGQLASY
                     HEITRSNWLIFIIIAVTTGSGAIFLYYYGLNRVKATVAVICELFFPISTLVFDYFING
                     TRLSAVQWASAALMVFAIINLNRTQQAENTRS"
     gene            5323..5748
                     /locus_tag="PKM34_04420"
     CDS             5323..5748
                     /locus_tag="PKM34_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ82864.1"
                     /translation="MVEDKWITLRNIARIEVRVGDVNYGGHMGNDRALLVFHDARIAF
                     LESLGFSEKNIGGPAIIMRDAHVNFRKEVFLHDVLTVDVGIDEVTTSSFNMTYTVKRE
                     SDGAVVFLGSTGLVAFDYETRKVVKLPEVFIQRIVKSEE"
BASE COUNT         1626 a         1447 c         1414 g         1541 t
ORIGIN      
        1 acaaccggat tgtctttctc ccagggggct ccacccaggt cgaaattggg gaaccattgt
       61 tcttccgtct caaggtaagc gctctcaata ttgaacatgc catcgtgcgc aataaaagct
      121 ttgaaccgtc tctcatgatg tccggccagc cagtaaacgg aaaatccgcc ataactggct
      181 ccaacagcgc ccaggcgatc tttatcgaca taaccctcgt ttgaaacatc atcgatcgcc
      241 gccagcaaat cctgcatgtt ctgtccgcca tagtcaccgc tgatctgttc tttccaggcc
      301 tggccgaatc ccgggacgcc ccggcggttc ggggctacca cgatatagtc gtttgcagcc
      361 atcatctgaa agttccagcg gtacgaccag aactggctca gggcattctg cggaccaccc
      421 tggcaataaa gcaatgccgg gtatttgttc cgctcgtcaa aatggggcgg ataaattacc
      481 caggtcagca tcttcttacc atccgtagtg ttgacaaacc gcttctcaac cttccccagg
      541 gagagctggt cgaggatgtc tttattggtg aaggtgatct gtgtctcttt tccgccggca
      601 gcaggtacgg cgaaaagctc ggcaggcatc gacatggtcg tcatcgaagc aatgatcttg
      661 tcgcctgcag gctgggcagc agtgaaatcg tggacgccac tgctgagctg tgttatcgta
      721 ccattgaggt gaagattaaa aatttccttg gatccgtacc aataagcaac aaaatagatc
      781 gactggctgt cgtcggccca taccgggccc tcggcgtcat tatcgaaaat tcccgtataa
      841 tcgacgcgtg aattgctctc caggtcgaaa accattaacc ggctcttatc ggcttcataa
      901 ccgtcacgtt ccatactggt ccaggcaatg tgtcttccgt caggcgagaa tacaggatca
      961 ttgtcataac ctttcatatc acgtgtcagg agggtctgct gcctggtttc caggtcataa
     1021 atgaagatgt cggtattggt ggaaacagcg taggcttttc cgaaattctt atggcaggaa
     1081 taagccagct ttttcccatc ggggtgccag ctcatctgct ccatgccgcc aaaaggcttg
     1141 ttgggaaagt caaacggttc cccttccaga atatccgtta cgttggataa ttttccgtcc
     1201 tggaaatccg cgataaaaag atgactgtat gtatcacgcc attcgtccca atgcctgaac
     1261 atcatcgaat gataaacctt cccggaggtc aacggtaatc cttcaaacaa atctttcttg
     1321 tattgatcat ccaggtttac ctcagccgag taagctattt tcttcatatc ggccgaaaat
     1381 ttaaagccgc tgatgccgcc ggggatatcc gtaagctgct ttctgcccga tccatcggga
     1441 ttcatttccc aaacctgaac agaaccactg gctgtgccgg caaacaggat cttctgccca
     1501 tccggccgcc acgtgtagtt gaattcgttt tcgggtgttt ttgttatctg cttcagattc
     1561 ttgccgtcag ttccgatcag gtatagttcc cggttacttt tattcctgtc aatatcataa
     1621 taagtgatgc tgaacagtat tttctgaagg tccggtgaaa cttcaaactc gcccacacgc
     1681 ccaaacgacc ataatacctc cggcgtcata cggtctgtgg ccagttttaa ctcttttttc
     1741 ccgatcagag gttctttgtt aacggattcc gtgcacgatg aaaggatcat cacaaggatc
     1801 aggctgaaaa caaaggtgtg caagtatttc atcatcggct cttcttttat agtgagtggc
     1861 aaaattaaga attaatcgct attcattctg atcatcaacc gggcaatgat cgaatcccag
     1921 atgtaattgg gggggcaatt caggacttca ttgtccgggc tgtatgattc cgggaaccgg
     1981 tggtttctcg aaagctgcac tttaacagca cccagtaaat tcacagccag ttcttccgcc
     2041 aggctgtcat agccgtaatt cctcaaccct tcataaacca tgtaatccca taacagccag
     2101 acgggcccgt tccacttgca gcaataatcg acataagggc tgtaccattc atcatcggca
     2161 gccagcgtag gcacgccgta tttcctccag aatttgccgg gatcggtcaa tgtttgcacc
     2221 agccggacgg ctcttgtgct gtcggcaact tgtgtccaga gcggcagaaa accgatgatc
     2281 tcctgccgtt tgaggtccct cgtcataaaa tagaagctgt ggtcagtctt gtcgaccgaa
     2341 taatagaatc ccgacgcatc atcccacatt ctttcattga tcaatcgggc cagtttgttt
     2401 gctttacttt cccactcctc actctcttct cgtaaatcaa gcgctgtagc catcgcggcc
     2461 aggctccgca tttccttaac catcatcagg ctgagatcaa ggcattcgag ctcatccgaa
     2521 atatcctcct tgtcgatatc caggtaacgc tccttcgaaa cctggaatac ggcattatac
     2581 caatccctca cattttcaat gattccatat gggccccatt cgaaagtccc gtcatgatcc
     2641 gtgtcccggt tattgatcag ccaccgcgca taccgggtgc cgctctcgta agcgtcagca
     2701 agaaactccc tgtcatggct cacattataa atctccaggt ttatccacga atagaacgga
     2761 gccgatgtgg tgggcatttc tttatgcgga tatgtctgcg gcccccgagg tccgtggcgg
     2821 taagcaatca aaccgtcttc gctttgctgt tccatatata cccgttgtga attctgtgcc
     2881 gaaacaggat caaggtaggc ataggccagc atgctgagcg attcatgcag gacctggtgg
     2941 ccgtgtcccc agccccagag cggttcccgt gagaaaacat aataattatg ggtagtctgt
     3001 ccttcggccg gcaaaaagca gcccctggcc aggttgaaag ccgaaaggta aaccatctta
     3061 tccgcaggat cactgaagtc gatcagtgga atattggaaa acaatctgac attttcatca
     3121 ataaatggct gaatggaaag gctctttgca tcttccagga ttttgccaag acgttcgggc
     3181 tccttgtccc ggctctgtcg caaccggcaa taacggtaat ggattgtagc gcccggttga
     3241 aggatgtatt ttccatgcag ggctatgaaa ccggcatcac tgctgtctgc catattgagg
     3301 gaatccgacc tgttttcaat gtaaaagtcg gtcttgatgg cattgtacag atcctccctg
     3361 ttaccttgat acgcaccgta agagtatatt gggaacccgg ctgaaaaaag gttataaaaa
     3421 tggtcgggat aaggatattt atcggataga ttgctgatca gccttttttt tgtttcgtaa
     3481 tgattaaaac aataactatt tgaagtagag tcaaatccaa tgacataaag ggaatcggca
     3541 tcttccagta cggggtaaat attgatctcg tgggtgtttg gtgtattgtt tttcagatga
     3601 caatccacaa cagcacaatt cgaattatat acgaaaaaga tctcttcagc gctgattccg
     3661 ggccatggct cgtattccat gagcgccata tccggaaaag tagctttgat gaacggtttt
     3721 acatgaaaat cgccgacccg gctgactacc acgctgttga tttcccaaac agtgaaaaac
     3781 cttccgccgt gttcactgcc gtacgttaaa ggggatgaga attcatcata gatcatcgta
     3841 taacccttgt cgtcgtaaag agatgagcgt gccggcgccg ctgtatatgt agtataaaca
     3901 ggatcgccgg gaccggcatt gatgatcagg tacccgtcgg caggaagaag attctgggcc
     3961 gacaggaaaa cagatataca ggccccgaga cctgtcgtgg ttagcttccg tatcaacgat
     4021 aaattatttt ccggccaggt attcttcgac gatcttcctg gcttcttcct catggctttc
     4081 aataacataa accaggccgg catcttccgg gcctcccgaa acccagccgg caacaaggct
     4141 ttctcccatg gtattgcgga ccatcgcgcc gataccgctc tcttccagca aactcttaat
     4201 ataattagct tccaatacgg aaccggtaaa tacattcttt aaactttcct gttcgctcat
     4261 agctcatagt tttaagtctt agacaaatat aaggaaaaag caaagaaaag caaaatcatg
     4321 aacgtgtatt ttcagcctgt tgtgtacggt tcaggttgat gatggcaaat accatcaggg
     4381 cggcgctggc ccattgaacc gctgaaagcc tggttccatt gataaaataa tcgaagacca
     4441 gggtcgagat agggaagaat aattcgcaaa tcacggctac agtggctttg acccggttca
     4501 gcccgtaata ataaaggaag atggcaccgc tgccggtcgt cactgcaatg atgatgaata
     4561 tgagccagtt ggagcgggtt atttcatgat atgaagccag ttgcccggta atgatcacat
     4621 acaacagcat gatgacagtg gtaaacccgt accggtaaaa agtggcggta ctgaatgaat
     4681 acctcctcag gatcattttg ctgaagacgg tggaactgcc gaacgagaag gctgccagaa
     4741 gggcaaacaa cgctgataat gcagtatttg acccggtgtg cacatccgga attttgaagc
     4801 cgaatgtcat gaaatagcca cccagcaagg caaccgatgc ccagaggata aaatatcggt
     4861 tgatcctctc tttcaataac aacgtggcca gcagtatggc aaacaccggc tggagtttct
     4921 gtagcaggac cacgatgctt aactccttga aattgatcag aaagagggct tttacaatgg
     4981 ataaagtacc gattgcacca ccgaataagg ctatcagcag taataaaata taatcatttc
     5041 gccggaatgt tttcaggtga cggtattccc ggaaaagaaa aagctgcatt aacaggaacg
     5101 ggatcaggtg caggataaaa acgacaagcc cgacatccag gttgtacagc ctgggcgtta
     5161 gcacaacacc gtccaggccc cacatgatgg ctgcaaaact aatggccgtt gccccgataa
     5221 tgaatgcttt tcttcccggc atgattccct gaatagggct gcaaagatag ttttaagtct
     5281 taaatattgt atttttgatg ataatgatat gatgaaaaga atatggttga ggataaatgg
     5341 attaccctga ggaatattgc caggattgaa gtcagggtcg gagatgtcaa ttatggcgga
     5401 catatgggaa acgacagggc attgctggtt ttccacgatg cccggattgc ctttctggaa
     5461 tcgctgggat tcagtgagaa aaatatcggc gggccggcca tcatcatgcg cgacgcccat
     5521 gtgaacttcc gtaaagaggt gttcctgcac gatgttttaa cggtggatgt cggtattgat
     5581 gaggtgacca catcttcatt caatatgacc tataccgtaa aacgggaaag tgacggcgcg
     5641 gtcgtattcc tgggcagcac cggcctggtg gcatttgatt atgaaacacg aaaagtggtt
     5701 aaactgccgg aagtctttat tcaacgaata gtgaaaagtg aagagtgaaa agtgaagagt
     5761 gaagagtgaa gagtgaagag tgaaaagtga attggttaac aatagaacaa tagaacaata
     5821 gaacaataga acaatagagc aatagaacaa tagagcaata gaacaataga acaatagaac
     5881 aatagaacaa tagaacaata gaacatatac catgaaaaac ctgcttgcga tactgttttt
     5941 gtttgctgtc ggtggcctgg cggcacaggt gacttttgta atcgatgcat tgccggaata
     6001 tactcctcct gacgactaca tttatatc
//
LOCUS       DAOQXS010000110         5986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13538_length_5986_cov_3.903220, whole genome shotgun sequence.
ACCESSION   DAOQXS010000110 DAOQXS010000000
VERSION     DAOQXS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13538_length_5986_cov_3.903220"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKM34_04425"
     CDS             complement(<1..477)
                     /locus_tag="PKM34_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82865.1"
                     /translation="MKYKAFRIFLLAFLVSSLCLAQGPLSVNPVADPDQICTGEYSQL
                     FAFASGGSGIYTYQWSSNPPGFSSDLPDPIATPYQTTTYYVSVNDGYNYVSGSVTISV
                     FSHPIPDAGPDQAIPHGTSTMLYGSASGGSGNYIYLWEPANKLVNPNVAIPSTVNLT"
     gene            complement(556..1446)
                     /locus_tag="PKM34_04430"
     CDS             complement(556..1446)
                     /locus_tag="PKM34_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ82866.1"
                     /translation="MNALITVIVIVVVFLFLAGIRIVRPTHRGLIERLGKYNRFANAG
                     FNWIIPIIERMYQVNVTEQMVDAEPQEIITNDNLNARVDAQVYFRVKPDEENVKNSQY
                     NVNNYKWQIVNLARTTLRNIIGTLTLKSANSERGKINSELHKTLMEETHSWGIDIVRT
                     ELKEIDPPKDVQETMNKVVKAENEKIAAIDYATARETAADGEKRAKIKEAEGIKQSRI
                     LQAEGEAEAIKLVNEAADKYFTGNAQLLRKLEALESALSKNAKIVVPAGSELVNVTGD
                     MAGVVPLPVGKKEGDGGVHK"
     gene            complement(1481..2047)
                     /locus_tag="PKM34_04435"
     CDS             complement(1481..2047)
                     /locus_tag="PKM34_04435"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNQ82867.1"
                     /translation="MTDFKDLQIKRYDSVGGWLLLLCLALTIFSPIRTVYNLVTTYEE
                     INQLFTTYPGLQTVYYIDMVVSIVLMLFSIRAGYMLWNIKPGAVKIAKNYLLLFLAYS
                     VFAAFLPFTAGLPSEANDLMIPEVIKGILQGVFFFVVWYWYLSVSKRVKATYVTDIIY
                     TENEVVEEAGSSTEIADYQDKEEEAENK"
     gene            complement(2321..2800)
                     /gene="tnpA"
                     /locus_tag="PKM34_04440"
     CDS             complement(2321..2800)
                     /gene="tnpA"
                     /locus_tag="PKM34_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNQ82868.1"
                     /translation="MANTYTQCHIHLVFAVKYRQALIKLEWKDMLEMYITGIIRNHGH
                     KLLAIGSMPDHIHIFIGYNVNHLIPDLVEEIKTSTNAWINRKKLSRNKFAWQRGYGAF
                     THSHSNISIVANYIENQPAHHRKKTFRTEYLEILAKNEIKYNSEYLFDFFDDLESWE"
     assembly_gap    2912..3011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3416..5176
                     /locus_tag="PKM34_04445"
     CDS             3416..5176
                     /locus_tag="PKM34_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82869.1"
                     /translation="MKSLKILFSISILTAFLCLNDANGQWLTNGTHIYNSNTGNVGIG
                     HSTPSKLLSVGKSMTEPTVMVRNLGGTGGATFEMIDDASGADWKFKATLSGGFKIRDN
                     LNSADKIIIEPNAYPNSIYIGAFGRVAIRHTAPERELDVSGDAMIRGYFPFLSFDNEG
                     SEHAGIVFYNGDDYRGYIYYDEIDQLIRISANQSSIANDALVVKYPNLVGIGTASPDA
                     LLHIHKDIYNSSYFGYLDTRANYFTHREIEADGNGQSCVYASRNRDVRNDGTGYLAIQ
                     SNNAIVGSCTWGDLYSFAVFGTNYNDYGRCGGVLGIESDNEGNWGSLGYTSSGLTAYG
                     GYFTTHTSGAGKSDSDLYTTVGMGAWGDLFGADIHGKVYGAYVEGSHYAGYFHGDVIK
                     DKLDVHLQKNATGTNDVLYTSVSADVTVVTSGTAMLSGGKASIAFDPVFSSAVSASES
                     VVVTVTPIGESAGIHLTKVSASGFSMAENNAGKSDVMVNYIAIGKRAGHENPDPAKEV
                     IAPDYTGKLSRGLHADGNTETDGEGLYFERGELVVGIHPTLLPDPNRSTVEETIIPEQ
                     KSPVKNGVADDGRLVGDDLK"
     gene            5419..5778
                     /locus_tag="PKM34_04450"
     CDS             5419..5778
                     /locus_tag="PKM34_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82870.1"
                     /translation="MDEAWLLMHKKAEVWGLWVFLNDLAGGYKSYVLGGMGDDFSNPK
                     VLARCSIFECMIYSFGDKTRLCLYDDNLNVIEQKDSEYDLLGFGVFEVGNIHLFYYNR
                     QNDVFEIWLDCNADPED"
     gene            5807..>5986
                     /locus_tag="PKM34_04455"
     CDS             5807..>5986
                     /locus_tag="PKM34_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82871.1"
                     /translation="MKRKATAIWQGNGMEGKGSLNSVSGVFKGTPYSFVTRFKNEVGV
                     EGTNPEELIASAHGGC"
BASE COUNT         1538 a         1324 c         1375 g         1649 t
ORIGIN      
        1 tgtcaggttc acagtcgatg gaattgcaac attcggattg accagcttat tcgccggttc
       61 ccacaggtaa atgtaatttc cgctgccacc cgaagcgctt ccatataaca ttgtgcttgt
      121 cccatgtgga atggcttgat cggggccagc atccggaata ggatgcgaaa agacgctgat
      181 agtcaccgat ccggaaacat aattataacc gtcattgaca ctgacataat aagtagttgt
      241 ctgataagga gtagcaatcg gatcaggaag gtcactcgaa aaacctggag gattggatga
      301 ccattgatag gtatatatcc ctgatccgcc ggatgcaaag gcaaatagct gtgaatattc
      361 tccggtgcaa atctggtcag gatcagccac cggatttacc gacagcggcc cctgggctaa
      421 acacagggat gaaaccagaa aagccagtag aaaaatgcga aacgctttat atttcatttt
      481 aattatgatt gtcatattgt ttcaatgttc caatgttcaa cgactgccta ctgcctacta
      541 cctactgtca actgcctact tatgcacccc tccgtcgccc tcttttttgc ccaccggcag
      601 cggcaccacg cccgccatgt cgccggtcac attgaccagc tcggagccgg ccggcaccac
      661 gatcttggca tttttcgaca gggccgattc gagcgcttcg agcttacgca gcagctgggc
      721 attgccggtg aagtacttgt cggccgcttc gttcaccagc ttgatggctt cggcttctcc
      781 ttccgcttgc aggatgcggc tttgcttgat cccctcagcc tccttgatct tggcccgctt
      841 ttcgccgtcg gcggccgttt cacgcgccgt cgcatagtcg atcgcagcga tcttctcgtt
      901 ttccgccttc accaccttgt tcatcgtctc ctggacatcc ttgggcggat cgatctcttt
      961 cagctcggtg cggacaatat cgatccccca gctgtgcgtc tcttccatca gcgttttatg
     1021 cagctccgag ttgatcttgc cgcgctcgct gttggccgat ttaagcgtca gcgtgccgat
     1081 aatgttacgg agcgtcgtcc gggccaggtt cacgatctgc cacttgtaat tgttcacgtt
     1141 gtactgcgag ttcttcacat tctcttcatc cggcttcacc ctgaaataaa cctgggcatc
     1201 gacacgcgcg ttgagattgt cattggtgat gatctcctgc ggttcggcgt cgaccatttg
     1261 ctcggtcaca ttgacctggt acatccgctc gatgatagga atgatccagt tgaatcccgc
     1321 gttggcaaag cggttgtact tccctagcct ctcgataagg ccccggtgcg ttggcctgac
     1381 gatcctgatc cccgccagga acaggaacac aacgactatg acgataacgg taatcagtgc
     1441 attcatcttt ttaaatattt aaatgttttt ctaagcatcc ctatttattt tccgcttctt
     1501 cttctttatc ctgataatct gctatttccg ttgaactacc ggcttcctcc accacttcgt
     1561 tttctgtgta aataatatca gtcacataag tggctttaac ccttttggat acgctcagat
     1621 accaatacca tactacaaaa aagaagacac cctgaaggat tcctttaatc acttcgggta
     1681 tcatcaggtc attggcttcc gaaggcagtc cggcagtaaa tggtaaaaaa gccgcgaaaa
     1741 ccgaatatgc caggaaaaga agaaggtaat tcttggcaat tttgacagct ccgggtttaa
     1801 tattccatag catatatccg gccctgatac tgaacagcat cagcacaatg ctcacaacca
     1861 tgtcgatgta gtacacggtt tgcaatccgg gataggtggt aaaaagctgg ttgatttcct
     1921 cataagtcgt taccagatta taaactgtac gaatcgggct gaagatagta agggcaaggc
     1981 ataataacaa cagccatcca ccgacgctgt cgtatctttt aatctgtaaa tctttaaaat
     2041 ctgtcatgtt ttggctcctt tcaattaagt ttggttaaat atgcgatgat ttgattgcct
     2101 ggctataaca tcctcaaatt taatgaaata ttggtattgc gacataaaaa atcacgattt
     2161 aatttttccg cggtgcagcg caccgtccta actgtaacaa cgaacctgcc aaaaccttca
     2221 ggtgcagcgc accgatagac atgtaaccaa cgaaaacgca gccataccca aaggtgcagc
     2281 gcaccgtaac cgtagcacac gtgaactaca aatgaagaat tcattcccat gattccaggt
     2341 catcaaaaaa atcaaataaa tattcggaat tatacttgat ctcattttta gcaagaattt
     2401 caagatattc cgttctgaat gtctttttcc ggtgatgtgc aggttggttc tcgatataat
     2461 ttgccacaat ggagatattt gagtgagaat gggtaaacgc gccataacct ctttgccagg
     2521 caaatttatt ccttgaaagt tttttccggt taatccaggc atttgtcgag gttttgattt
     2581 cttccaccag atctgggata aggtgattga cattgtaccc gataaaaatg tgaatatgat
     2641 ccggcattga gccgattgcc aggagtttat gcccgtggtt ccggatgatg ccggtgatat
     2701 acatttccag catatctttc cattccagtt taattaatgc ttgcctgtat ttaactgcaa
     2761 acaccaggtg aatgtgacat tgtgtatatg tatttgccat aattgattgt atttcggtgc
     2821 gctgcacctt tgattattat tgcgtttatt tgcctacaga tgttcgggtg ctacgcaccg
     2881 gggtcgtgtg gttatcagat gttcgggtgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn ngttcgggtg ctacgcaccg gggttgtgtg gttaacagat gttcgggtgc
     3061 tacgcaaccg gggttgtgtg gttaacagat gttcgggtgc tacgcaccct aatcgaaggt
     3121 ggaaaggtcc aactactata gccgcggtgc agcgcaccgt cacacctgta accagcgagc
     3181 gcaccacctt gatctaaggt gcagcgcacc gtcacaacca taggattcaa tatattaatc
     3241 cgttttttcg gagaaaaata cccggtcagc agaaaaaata atttgaatat atctccaaac
     3301 caatcgaaat caaagatttc ctgcccgctc tattgacatt ttgtgagaag ttacttattt
     3361 ttacctgtcg tttagtttaa tataattatc ctccaaacca tttaatcaaa tccttatgaa
     3421 aagtttaaag attttgttca gtatttccat tctgacagca tttctttgtc tgaatgatgc
     3481 aaacggccaa tggcttacca atggcaccca catttataat tccaataccg ggaatgtggg
     3541 tatcgggcac agcactcctt ccaaactcct ttctgttggg aaaagcatga cggaaccgac
     3601 cgttatggtg cgtaatcttg gcggtaccgg cggcgctacc ttcgagatga tcgacgatgc
     3661 cagcggcgcc gactggaaat tcaaggccac cctcagcggt ggatttaaga tcagggataa
     3721 cctgaacagc gcagataaga ttattattga acccaatgcc tatcccaatt caatttatat
     3781 tggagcattc ggacgtgttg ctatacgtca tacagcgcca gagcgcgaac tggatgtcag
     3841 cggcgatgct atgatcagag gctatttccc ttttttgtct tttgacaatg agggatcaga
     3901 acatgccggt atcgtttttt ataatggcga tgattatagg ggatatattt attatgatga
     3961 gatcgatcaa cttataagga tctcggctaa tcaatcttct atcgcaaatg atgccctggt
     4021 ggtcaaatat cctaatctgg tcggaatagg gacagcatca ccggatgcat tgctgcatat
     4081 tcataaagac atttacaatt caagctattt cgggtatttg gatacacgtg caaattattt
     4141 tacccaccgt gagatcgaag ccgatggcaa cggtcagtca tgtgtgtatg caagccggaa
     4201 cagggatgtt cgaaatgatg ggaccggtta cctggctatt cagtcgaata acgctattgt
     4261 cggttcctgt acctggggcg atttgtattc attcgctgtt ttcggaacaa actacaacga
     4321 ttatggaagg tgcggagggg tcctgggtat tgaatccgat aatgagggaa actggggctc
     4381 attgggttat acgagcagcg gattaacggc atatgggggc tattttacaa cgcatacctc
     4441 aggcgccggc aaaagcgatt ctgatcttta taccaccgtt ggaatgggcg cctggggcga
     4501 cctcttcggc gccgatatcc acggaaaagt ttatggagcc tacgtggaag gcagccatta
     4561 tgcggggtat ttccatggcg atgtcattaa agataaactg gatgtccatc tccagaaaaa
     4621 tgccactgga acgaatgatg tcctctatac cagcgtcagt gccgatgtaa ccgtcgttac
     4681 cagcggaacc gcgatgcttt cgggcggaaa agccagcatt gctttcgacc cggtgttctc
     4741 atccgctgtc tccgccagcg aatctgtggt tgttaccgtt actcccatag gcgaatctgc
     4801 aggtattcac ctcacaaaag tatcagcttc gggtttctcc atggctgaaa ataatgccgg
     4861 gaagagcgat gtgatggtca attatatagc cattgggaaa agagccggcc atgaaaatcc
     4921 tgatcctgcc aaagaagtca ttgcacctga ctatacgggt aaattatcac gaggccttca
     4981 tgcggatggc aataccgaaa ccgatggcga agggctttac tttgaaagag gtgaactggt
     5041 tgtcggtatt catccaaccc ttctgcccga tccgaaccga tcaacggtgg aggaaacgat
     5101 cattccggaa caaaaatccc cggtgaaaaa tggtgttgct gatgacggaa ggcttgttgg
     5161 cgacgatctg aaatgatata atcgttatct ttgaaggtta ataatcaagc catgagaaat
     5221 ttattgttgt cacttttaac cttcctgtta ccttttattg catcaagcca gcccatgagc
     5281 tccgttgcat ttttcccaga aaactgggcc attccttcga tggataatgt tccggaaaac
     5341 agcggatggg attttctgat ccgggacgga aacgataaac cgtattatgt ctttggtgat
     5401 tttaacgggg ataattttat ggatgaagcc tggctgttaa tgcataaaaa ggcagaagtt
     5461 tggggtttat gggtttttct gaatgatctg gccggaggct acaaaagtta cgttctgggg
     5521 ggaatgggtg atgatttttc caatccaaag gttttggcga gatgttcaat ctttgaatgc
     5581 atgatatatt ctttcggaga taaaacccgg ttatgtttgt atgatgataa tttgaacgta
     5641 attgaacaaa aagactctga atacgattta ttgggttttg gcgtttttga ggtaggaaat
     5701 atccatctgt tttattacaa cagacaaaat gatgtttttg aaatatggct ggattgtaat
     5761 gccgatccgg aagattaaaa attatttaac aaacttataa ttcagtatga aaagaaaagc
     5821 aacagccata tggcagggaa acgggatgga aggcaaaggg agcctgaact ccgtcagcgg
     5881 ggtatttaaa ggcactccgt attcatttgt aacgaggttt aagaacgaag tgggagtgga
     5941 agggaccaac cccgaggagc tgatcgcgtc ggcgcacggc ggatgt
//
LOCUS       DAOQXS010000111         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13547_length_5983_cov_3.679150, whole genome shotgun sequence.
ACCESSION   DAOQXS010000111 DAOQXS010000000
VERSION     DAOQXS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13547_length_5983_cov_3.679150"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKM34_04460"
     CDS             complement(<1..210)
                     /locus_tag="PKM34_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ82872.1"
                     /translation="MLLRRVYDDGLAQASYFVACPQTRQAILFDPARDIDRYLLDAEQ
                     AGLTIAAVAETHLHADFLSGMNAFAA"
     gene            261..773
                     /locus_tag="PKM34_04465"
     CDS             261..773
                     /locus_tag="PKM34_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82873.1"
                     /translation="MLLLVRGAHLAAKPCVALGHHRVAEADDEHAQLEQPPAHGDGLG
                     PGDNGYKFLKKVFSCRFNQYLFRWLHPDIGARMGLYFSGKSRLANMIKEDKAENLNRI
                     EDEMLYQYATGILQNGTKPDYFIFGHRHRPENIELPGGSRLIILGDWLTHFTYAEFDG
                     KQIHLKKFKS"
     gene            777..2546
                     /gene="sppA"
                     /locus_tag="PKM34_04470"
     CDS             777..2546
                     /gene="sppA"
                     /locus_tag="PKM34_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNQ82874.1"
                     /translation="MKDFLKFMLASMLGFVIILTLSFFILIGIFASIASMADKKSVSI
                     ESSTVLQIKLENEVFDRAPKDPMTFFSPAGFTMEQEPGLDEILKNIKKAQTDDKIKGI
                     FLDLSVVPSGLATVREIRNALIEFKSGGKFIIAYGEVYSQMAYYLGSVADKMYLHPAG
                     AIDFRGFGAQVFFLKGTLEKLDIEMQVIRHGKYKSAVEIFTEDKMSEASREQMNAMVT
                     DFWATALADISASRNISLARLNEIADNLDAYEAGKALNIGMIDGLYYRDQLLDELRNK
                     LGLGEKEKVRTVSLAKYTLVTPATPQKVSANKIAVVYALGNIIDGKGDDRTVGSVNIS
                     EAIRKARENDKVKAIVLRVNSPGGSASASEVIRREVVLAAKEKPVVVSMGDVAASGGY
                     WISASASYVMADPATLTGSIGVFGMIPNMQGLFNNKLGITFDEVNTNKYSDFPNVSRP
                     MSDYEILVIERQIEKIYDDFLSLVAEGRKMEKSQVDEIGQGRVWSGSDAKEIGLIDEF
                     GGLTAAIDKAAGLAGITEYNLMSLPAQKEPLQQIIDELTGNVAAGRIEKELGEYYTYY
                     DYIKQIKEASGIQARLPFEVKIK"
     gene            complement(2558..5982)
                     /gene="nifJ"
                     /locus_tag="PKM34_04475"
                     /pseudo
     CDS             complement(2558..5982)
                     /gene="nifJ"
                     /locus_tag="PKM34_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
BASE COUNT         1455 a         1560 c         1514 g         1454 t
ORIGIN      
        1 cgcagcgaag gcgttcatgc cgctcaggaa gtcggcgtgc aggtgcgtct cggccacggc
       61 ggcgatggtc aacccggcct gctcggcgtc gagcaggtag cggtcaatgt cgcgcgcggg
      121 gtcgaacagg atggcttgcc gggtctgcgg acaggccacg aagtagctgg cctgcgccag
      181 gccgtcgtcg tagacgcgtc gaagcagcat caggcgaatt ccttcttgag cgcgcgcagc
      241 gcggtgccaa gcgcgtccag gtgctgcttc tggtgcgcgg cgctcacctg gcagcgaagc
      301 cgtgcgtggc cctcgggcac caccgggtag ccgaagccga tgacgaacac gcccagctcg
      361 agcagccgcc ggctcatggc gatggccttg gcccgggcga taacgggtac aaattcctca
      421 aaaaggtatt cagttgccgg tttaatcagt atctcttccg ctggctgcat cccgatatag
      481 gcgcccggat gggactctat ttctcgggaa agagcagact ggccaacatg atcaaagaag
      541 ataaagccga aaatctgaac aggattgaag atgaaatgtt atatcaatac gcaaccggta
      601 ttttgcaaaa tggaaccaaa cccgactatt tcatcttcgg gcacaggcac aggccggaaa
      661 atattgagct tccgggcgga tcccggctga tcatcctggg cgactggctg acccatttta
      721 cttatgccga atttgacgga aaacaaatcc atttaaaaaa attcaaatcc taggttatga
      781 aagatttcct gaaatttatg ctggcctcga tgttaggctt tgtcatcatc ctgacattat
      841 cattctttat cctcatcggg atcttcgctt ccatagcgtc tatggccgat aagaaatcgg
      901 tttccatcga gtcctcaacc gttctgcaga taaaactgga aaacgaagtt tttgaccggg
      961 ctcccaaaga cccgatgacc ttcttttccc cggccggatt caccatggaa caggaacccg
     1021 ggctcgatga aatcctcaaa aacattaaaa aggcccagac cgatgataaa atcaaaggca
     1081 tctttcttga cctgagcgtt gtgccgtcag gattggccac cgttcgggaa atccggaacg
     1141 ccctgatcga atttaaatcc ggcggtaaat tcataatcgc ttacggagag gtctattctc
     1201 aaatggccta ttacctgggg agcgtggccg ataaaatgta cctgcacccg gccggcgcga
     1261 ttgatttcag gggatttgga gcccaggttt tcttcctgaa gggcaccctg gaaaaactgg
     1321 atatcgagat gcaggtcatc cggcacggaa aatataaaag cgccgtggag atcttcacgg
     1381 aagataagat gagcgaagcc agccgcgagc aaatgaatgc catggtaacc gacttctggg
     1441 caacagctct tgccgatatc tctgcttcca ggaatatcag tctggcccgc ctgaatgaaa
     1501 ttgccgacaa cctggatgcc tatgaagccg gtaaagccct gaacatcggc atgatcgacg
     1561 gtttatacta ccgcgaccag ctgctggatg aactgaggaa taaactggga ttgggtgaaa
     1621 aggaaaaggt cagaaccgtt tccctggcaa aatacaccct ggtcacgccg gcgaccccgc
     1681 aaaaggtctc cgcaaacaaa atcgcagtgg tgtacgcatt aggcaatatt atcgatggca
     1741 aaggcgacga ccggacagtc ggatcggtca atatttctga agcgatccgg aaagcccgcg
     1801 agaacgacaa agtcaaagcg atcgtgctca gggtcaactc ccccggaggc agcgccagcg
     1861 catcggaagt gattcgccgt gaggtcgtgc tggctgcaaa agaaaaaccc gtggtcgttt
     1921 ccatgggtga tgtggccgcc tcgggaggct actggatttc ggcttccgcc agttatgtca
     1981 tggccgatcc tgccaccctc accggctcta tcggtgtctt tggcatgatc cccaatatgc
     2041 agggattgtt caacaataaa ctcgggatca ccttcgacga ggtcaataca aacaaatact
     2101 cagactttcc gaatgtgagc cggccgatgt cagattatga aatactggtg atcgaaaggc
     2161 aaattgaaaa aatttacgat gatttcctgt cgctggtagc cgaagggaga aagatggaaa
     2221 agtcgcaggt ggatgagatc ggccagggcc gtgtatggag cggatccgat gcaaaagaga
     2281 tcgggctgat cgacgaattc ggaggtttga ccgccgccat cgacaaagcc gccgggctgg
     2341 ccgggattac cgaatacaac ctgatgtcgc tcccggccca gaaagaaccg ttacagcaga
     2401 tcatcgacga gctgacgggt aacgtcgcag ccggaagaat tgaaaaggag ctgggtgaat
     2461 actacaccta ttatgactac atcaaacaaa taaaggaagc cagtggcatc caggccaggc
     2521 ttccttttga ggtgaagatt aaataatttt cagaaggcta cttctccgaa aaatccattc
     2581 cggccagcct cttgtaggtt gtgtatctcc atctggcatt ctgttcggcc agggcatcca
     2641 aacgtgcagc ttcgctcggg aaggctttct tgagcgaagc ataacgcacc tcgccgctga
     2701 ggaatccctg gaactggctc cagtcaggct cttttgaatc gagctggaac gggttctttc
     2761 cctctgcttc caggagcggg ttgtaccggt ataggtgcca gtatccggca tttaccgcca
     2821 gttcctgttc gtattgcgtc ttgcccatac cgttgcggat accgtggttg atacagggcg
     2881 aataagcgat gatcagcgac gggcccggat atgattccgc ttccttcagg gctttgaaga
     2941 actggttctg gttagcaccc atagctacct gcgcaacata aacataaccg tagctgatag
     3001 ccatgatacc caggtctttc ttgcgggtct tctttccgga tgcggcaaac ttggctaccg
     3061 cgccaaccgg tgtggatttc gatgcctgtc ctcccgtatt tgaatacact tctgtatcaa
     3121 gtaccagcac attcaggtct tccccgctgg ccagcacatg atccaggcca ccataaccga
     3181 tatcgtaagc ccagccgtcg ccaccgacaa tccagatcga ttttttcacc aggtactgtt
     3241 tcaggctgag gatctcggct gccaccggat cagcagattt ctccaggagg ggaatgagct
     3301 ttttgctcgc tgcttccgat ccttcagcaa tattcatatt ttcaatccat tcgcggtaag
     3361 catcgcccag ctcgccatca atgcccgctt tgatcgaatc ctgcatcctt aatgcgatcc
     3421 gctcacgaag cttgctgata ccgatattta cgccgtaacc gtactcggca ttatcttcga
     3481 acagggaatt ggcccaggcc ggtccatgcc cgctgacatt gtgcggacgg tatggagtcg
     3541 agggggctga accaccgtaa atcgaggagc atcccgtagc gttggttacg atcatcctct
     3601 cgccatataa ttgtgtaata accttgatat aaggcgtttc accacatccg gcacaggctc
     3661 ctgaaaactc gaacaggggc tgcgcgaact ggctgttctt caccgactgg tctttcggaa
     3721 caatagtgtc tttataactg acattcttcg aaatgtaatc ccatctttca acttctccca
     3781 gttgcgattc gagcggttcc atcaccaggg ccttttcctt tgaggggcat acatcggcac
     3841 agttgccgca tccggtgcaa tccagcacac tcacctggat acggaaatgg tatccggcaa
     3901 atttgccctt ggtatcctgc gtggtcgtcc cttcaggggc gttcttcagt tcgtcgtccg
     3961 acagcaggaa tggcctgatg caggcgtggg gacaaacata tgcacactgg ttgcactgaa
     4021 tgcagttgtc caccttccat tccggcacat tgacggcaat acccctcttc tcaaattctg
     4081 tcgtgcccga catgaatgtt ccgtcttccc tgtcaaggaa agcgctaacc ggaaggtcat
     4141 cacctttaag attgttcacc ggaatggcaa tattcctgac aaaatcaggc acatctgcat
     4201 gtcctttatc atcaatgccg gccagttttt tccattctgc cggaatggtg accttaataa
     4261 cggcagaacc ggcatcaacg gcggcattgt tcatcttgac aatctcctct cccttattcc
     4321 cgaatgattt ccggatcatc cgcttcattt cgtcaactgc cgtttcataa ggaataaccc
     4381 cggcgatttt gaagaatgca gcctgcatga tggtattggt cctgtttcct aagccaatct
     4441 cttcagcaat ttttgtagcg ttgatgatat agaaattgat atcgttttct gcaagatatt
     4501 ttttgaacga ttcgggcagt ttgtgtttgg tctcttcctc atcccagatg ctgttcagca
     4561 ggaaggttcc cccctttttt agccccttca gcatatcgta tttattgaga tacgatggaa
     4621 catggcaggc tacaaaatcg ggggtggtaa ccaggtaagt agagtggatc ggcttatcac
     4681 cgaacctcag gtgagatgtg gtaataccgc ccgatttctt cgaatcatag gcaaaatagg
     4741 cctggcaata tttatcggtg ttgtcgccga tgatcttgat cgaattcttg ttggctccca
     4801 ccgttccatc actgcccaga ccgtaaaact tagcttcaaa cgtacctttc ggcacaacgc
     4861 tgaattccgg cagcaaaggc aatgaagtgt aggtcacatc atcgacgatg ccgaccgtaa
     4921 acctgttctt tggctcattc atcttcaggt tgtcaaacac ggagatgatc atcgacgggg
     4981 tggtatcctt cgagctgagt ccgtagcgcc ctccgacgat catcggggca tccttctttc
     5041 cgtagaaaat atccttgata tcgaggtaca aaggctcccc gtttgcgccg ggttctttgg
     5101 tccggtcgag aacagagatg cgctttacac tcttcgggag cacattcagg aaatattttt
     5161 cagagaaagg cctgtaaaga tgtacggtta ttaatccaac cttctctcct tttccgttaa
     5221 gataatcgat ggtttccttg atggtttcgg ttaccgagcc catggcaaca acgatatttt
     5281 cagcatccgg ggcgccgtaa taaacaaaag gatggtattc acggccggtc attttgctga
     5341 taatctgcat gtagtgttct accatatccg caataggcag gtagaaacgg ttccctgctt
     5401 cccgggcctg gaaatagata tccgggttct gcgccgtacc ccgggtcacc gggtgctccg
     5461 gattgagcgc acggttccgg aaagcctgga tggcttcgaa atccacaaga ggtatcatat
     5521 cctcattctc aaaatattca actttctgga tttcgtgaga agtccggaaa ccatcgaaga
     5581 aatggagaaa tgggatccgt gatttgatag cgaccaggtg agcaatggag gcaatgtcca
     5641 tgatctgttg cacgctgccg gttgccagca tggcaaaacc ggtatggcgg gtgctcatta
     5701 catcgctatg gtcaccgaag atggaaagcg catgggctgc caggctcctg gcactgacgt
     5761 gaaaaacacc cggtagcagc tcaccggaaa tcttgtacat attcgggatc atcagtaata
     5821 atccctgtga agccgtgaat gtcgttgtta aaacacccga ttgcagagaa ccgtgtacag
     5881 ctccggctgc cccggcttcc gactgcattt ctgaaacttt cagggtatcg ccaaatatat
     5941 tttttcggcc gatgccgacc attcatctac atattctgcc atg
//
LOCUS       DAOQXS010000112         5978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13557_length_5978_cov_3.455175, whole genome shotgun sequence.
ACCESSION   DAOQXS010000112 DAOQXS010000000
VERSION     DAOQXS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13557_length_5978_cov_3.455175"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1505
                     /locus_tag="PKM34_04480"
     CDS             162..1505
                     /locus_tag="PKM34_04480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNQ82875.1"
                     /translation="MKLKNVFSLITLAVILAVFATSCNIGTHPGYKKSKSGVYYKIHN
                     DNNDTTSVREGTIVTMDLKYGLKDSLMFDSKQSPNPIILQVAKSEYEGDFNECISLLN
                     QGDSATFILKAGPLFKTTFRQPELPEPLTDDSEIIFDVTIQKVQTQEQMEMETKLKNM
                     ELEKQEVIKLQEYIQKNNITAEPSSTGIYYIETDKGKGKSPAEETYLTAHYTVYLLDG
                     EQLFSTRERGEPVDFKYKSQYENEGFHEVLGMMREGGKAKAIIPSAMAFGEQGAGNIV
                     PPFSTLYYEIELIKVISKDEFNAKQQQKESKKKAEAEMKVKEEEANIQKYLKDNGLAP
                     TTTLPSGLVYIETKAGNGPKPETGKKVKVHYTGKLLNGNKFDSSLDRGEPFEFALGQR
                     QVIEGWDQGIALMSEGAKGLLVIPYRLGYKERGAGEDIPPYSTLVFEVELVEIEK"
     gene            complement(1567..2340)
                     /gene="cysQ"
                     /locus_tag="PKM34_04485"
     CDS             complement(1567..2340)
                     /gene="cysQ"
                     /locus_tag="PKM34_04485"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNQ82876.1"
                     /translation="MAVSAAIEGGNEILQVYGQDFAVEYKNDNSPLTLADRRAHEAIM
                     RILEPSGLPVLSEEGKAIGFETRRNWGQFWLVDPLDGTKEFVKRNGEFTVNIALIKGN
                     EPLAGVIYIPVTGVLYFACQTTGARKTVLEPSGKQSFDQIIKDSTILPESFSPDKLTV
                     VCSRSHMSAETEKYIDQLRQTHHDVDFVSIGSSLKLCLVAEGKAHIYPRFGPTMEWDT
                     AAGHAIALFAGCTVRQADSGSPVVYNKPDLKNPWFIVSR"
     gene            complement(2388..4427)
                     /gene="uvrB"
                     /locus_tag="PKM34_04490"
     CDS             complement(2388..4427)
                     /gene="uvrB"
                     /locus_tag="PKM34_04490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNQ82877.1"
                     /translation="MQFNLTSDFFPTGDQPEAIRQLKEGLDRGDPAQVLLGVTGSGKT
                     FTIANVIAQINRPTLVLSHNKTLAAQLYGEFRQFFPENAVEYFVSYYDYYQPEAYLPV
                     TNTYIEKDLSINEEIEKLRLSTSSSLLSGRRDVIVVSSVSCIFGIGNPDDFTRNVVKV
                     QIGDRISRNHLLYSLVDALYSRTETDFGRGRFRVRGDTVDVFPAYADIAYRIVFFGDE
                     IESIESFNPENGFKIEKQDNLTIFPANIFVTSQEKMKEALKEIEEDSWGQAAFFRDNG
                     KMFEAKRLEERVSYDVEMMRELGYCSGIENYSRYFDGRAPGTRPFCLIDFFPDDFLLV
                     IDESHVTIPQIRAMYGGDRSRKQTLVEYGFRLPSALDNRPLKFDEFESLVNQVIFVSA
                     TPADYELQRSGGVVVEQLIRPTGLLDPVIEVRPSQNQIDDLLGEIDITVKKGNRVLVT
                     TLTKRMAEELTKYLTRLGIKSRYIHSEVETLDRVEIMRELRLGKFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNIDSRVIMYADQITDSMRQTIEET
                     NRRREKQLAYNEAHNITPKAIIKSVERIMGQTAVADAKHKEPQPYFEKEEVEVAADPV
                     LKYLSDSQIQKSISDLRKEIEKAVKELDFIQAAKLRDEMYAMEEFLEDRKNDSKK"
     gene            complement(4443..5369)
                     /locus_tag="PKM34_04495"
     CDS             complement(4443..5369)
                     /locus_tag="PKM34_04495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNQ82878.1"
                     /translation="MQYYRNPLEEAGRFFRSGNLLSRLIIINTAIWLLVGVLRVISFL
                     FKFPDKELMEFLIDVMALPADTGILITRIWTLFTYMFFHLDFFHLLFNMLWLFWFGKI
                     FLEFLKPVQLLFLYLTGGLSGGILYILFYNLFPVFQESLPVSVALGASASVMAIVTAI
                     SVYVPGYTVHLLFLGRIRIFYIALFLFVLDFFMIRSENAGGHIAHIGGAVFGLIYILS
                     LRKGMNFNGVFKMRWFKQFTSIFGRRKMKVVSGNYRQTTERPLTDDEFNRRRAERQRK
                     IDDILDKVARSGYDSLSKEEKDMLFNASNSGD"
     gene            complement(5377..>5978)
                     /locus_tag="PKM34_04500"
     CDS             complement(5377..>5978)
                     /locus_tag="PKM34_04500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNQ82879.1"
                     /translation="NALENIWGPKRFLIYYFITGIGAALIHYLVFYFFDLSPVLSDIN
                     AIMAAPTYQAVSDFISGDPELFQIYLKEIYSDPQKFFGAMEILRTSPDNKQAIYIVKN
                     LLVDYREFYMNLPNVIGASGAVFGILLAFGMLFPNTLIYIYFFFPLKAKYFVIAYGLI
                     ELFYGFTGSGSNVAHFAHLGGMIFGFFLILYWKKKGRLY"
BASE COUNT         1748 a         1352 c         1300 g         1578 t
ORIGIN      
        1 ggaagctatt ttattgatga gggtaggcga cagggcaaaa tttattatac cttcgcacct
       61 cgcttttggc cttatcggcg accagaaaaa gatacgccat aaggccaccc tggtttatga
      121 tttggaattt gtcagcatga aatgaattta aaagtataat tatgaaatta aaaaatgttt
      181 tctcattgat caccctggcc gttattctgg cagttttcgc aacatcctgt aatataggca
      241 ctcatcccgg ctataagaag tccaaatccg gggtttacta taaaatacac aatgacaata
      301 acgacacgac atctgtaaga gaaggaacga tcgttacaat ggatctgaaa tacggattaa
      361 aggattcgct aatgttcgac agtaagcaat cccccaatcc cattatcctt caagtggcaa
      421 aatcagaata tgagggtgat tttaacgagt gtatttcact attaaatcaa ggcgatagcg
      481 ctacgttcat cctgaaagcc ggcccacttt tcaaaacaac attccgccag ccggaattac
      541 ccgaacctct taccgatgat agcgagatca ttttcgatgt tacgattcag aaggtccaga
      601 cccaggaaca gatggagatg gagacaaaat tgaaaaacat ggaacttgaa aaacaagaag
      661 taataaaact ccaggagtat atccagaaaa ataacataac agcggaacca tcatctacag
      721 gtatctatta tattgaaact gataaaggca aaggaaagtc accggcagaa gagacatacc
      781 tcacagctca ctacacggtt tatcttttag acggcgaaca attgttctcc acgcgtgaac
      841 gtggcgaacc ggtagatttt aaatacaaaa gccagtacga aaatgaaggc tttcatgaag
      901 tattaggtat gatgagagaa ggtggaaaag caaaagccat catcccttca gccatggcat
      961 tcggagaaca gggagccgga aatattgtcc ctccgttcag cactttatat tatgaaattg
     1021 aattgattaa ggtcatttct aaagatgaat tcaacgcgaa acagcagcaa aaggaatcaa
     1081 aaaagaaggc ggaggccgaa atgaaagtaa aagaagagga agcaaacatt cagaaatatt
     1141 taaaggataa tggacttgct ccgacgacaa cgctccccag cggcctggtt tatattgaga
     1201 caaaagcagg gaatggacca aaaccggaaa ccggcaaaaa agtcaaagtt cattacaccg
     1261 gtaagttatt gaacggcaat aaatttgatt cttcactcga caggggagag ccatttgaat
     1321 ttgcactggg ccagaggcag gtcattgaag gctgggatca gggtatcgcc ctgatgagcg
     1381 aaggagcaaa aggtttgctt gtaattccct accgtcttgg atataaggag cggggcgcag
     1441 gcgaagatat accgccatat tccacactgg tctttgaagt tgaactggtt gaaatcgaaa
     1501 aataaaccga aacgaatttg atatcgaaag aggctgcttc aattgtccgg acagcctctt
     1561 tttatttcat ctcgaaacaa taaaccatgg atttttcaaa tccggtttat tgtaaacaac
     1621 cggagagccg gaatctgctt gcctgaccgt acagcctgca aacaacgcaa tggcatgtcc
     1681 tgccgcagta tcccattcca tagtaggacc aaaccgggga tagatgtgtg ctttaccttc
     1741 tgctaccagg cataatttca atgagctgcc gatcgatacg aaatctacat cgtgatgggt
     1801 ttgccttagc tggtcgatat atttttccgt ttcagccgac atatgggagc ggctgcagac
     1861 gaccgttaat ttatccgggc tgaaactttc cggcaggatt gttgaatctt taattatttg
     1921 atcaaaagat tgtttcccgg atggttccag aactgttttc cgtgcgcctg tcgtttggca
     1981 ggcaaaatac aaaactcctg taaccggaat atatatcaca ccggctaacg gttcattgcc
     2041 tttgatcaga gcaatattga cggtaaattc gccattgcgc ttaacgaatt cttttgtgcc
     2101 atccagcggg tcaaccagcc agaactggcc ccagtttctt cgggtttcga agccaattgc
     2161 tttgccttct tcgctaagaa caggaaggcc tgaaggctca agtatccgca tgatagcttc
     2221 gtgtgccctg cgatcggcaa gcgttaaggg gctgttgtca tttttatatt cgacagcaaa
     2281 atcctgtcca tagacttgca atatctcgtt accaccttca atagcagctg atacagccat
     2341 ctctactaat cctaatatct cagggctggt catatgagga gggtctgtta tttttttgaa
     2401 tcattcttac ggtcttcaag gaattcctcc attgcgtaca tttcatcgcg aagcttggct
     2461 gcctggatga aatcgagttc tttcaccgct ttttcgattt ccttccttaa atcgctgata
     2521 gatttctgta tctggctgtc cgacaggtac ttcaataccg ggtcggcagc aacctccacc
     2581 tcttcttttt caaaatatgg ctgaggttct ttgtgttttg cgtcagccac agctgtttga
     2641 cccatgattc tctcaacgga tttaatgatc gcttttggtg taatattatg cgcctcatta
     2701 taagccagtt gtttttccct cctgcggttg gtttcttcta tggtctgcct catggaatct
     2761 gtgatctggt cagcatacat gataacacgg ctgtcgatat tccgggcagc ccttcctgca
     2821 gtctgggtaa gtgagcgggc cgaccggaga aacccttctt tatctgcatc caggatggca
     2881 accagtgata cttcgggtag atccagcccc tctcttaata agttaatccc aacaagcaca
     2941 tcgaacttgc cgagccggag ttcccgcata atctcaaccc tgtcgagcgt ttcaacttcg
     3001 gaatggatgt accggctttt aattccaagc cgggtcaggt attttgtaag ctcttccgcc
     3061 atcctcttgg tcaaagtggt gacaagtaca cggtttcctt tttttaccgt gatatcgatc
     3121 tctcccaaca ggtcatcaat ctggttctga ctcgggcgta cttcgatcac cggatcgagc
     3181 aggcctgtag gtcggataag ttgttccacc acaacaccgc cggatcgctg gagttcataa
     3241 tctgccggtg ttgcgctgac aaaaataacc tggttgacaa gggattcaaa ctcgtcgaat
     3301 ttcagcggcc ggttgtcaag ggcagaaggg agcctgaagc catattcaac cagggtttgt
     3361 ttacgtgacc tgtcacctcc gtacatcgcc ctgatctgcg gtatggtcac atggctctca
     3421 tcgatgacca ggaggaagtc atcggggaaa aagtcgatca gacagaatgg gcgggtacct
     3481 ggtgccctgc cgtcaaaata gcgggaatag ttctcaatcc cggagcaata ccccagttcg
     3541 cgcatcatct caacatcata ggatactctt tcttccagtc ttttagcttc gaacattttc
     3601 ccgttgtccc tgaaaaaagc agcctggccc catgaatctt cctcaatctc tttcagggct
     3661 tctttcatct tttcctgcga tgtcacgaaa atattggcag gaaaaatggt caggttatcc
     3721 tgtttctcaa ttttaaaccc attttcgggg ttaaaggatt caatactttc aatttcatcg
     3781 ccaaaaaaca cgattcgata ggcaatatcg gcataggccg ggaaaacatc aaccgtatcg
     3841 ccacgcacgc ggaaccggcc ccggccaaaa tcggtttccg ttcttgaata cagggcatcc
     3901 accagggaat ataaaagatg gttcctgctg atccggtcac caatctgtac cttgactaca
     3961 ttccgggtaa agtcatccgg atttccgata ccgaagatgc aggaaacgga tgaaacaacg
     4021 attacatctc ttctgccgga aagaagcgat gaggatgtgc ttaaacgtag tttctcgatc
     4081 tcttcattga tggagagatc tttttcaatg taagtattgg taacaggcaa gtaagcttcg
     4141 ggctggtaat aatcataata agagacaaag tattcaacgg cattttccgg gaaaaactgg
     4201 cgaaattctc cgtaaagctg cgctgcgagt gtcttattat ggctcagtac aagggtcggc
     4261 ctgtttattt gagcaataac attggcaatg gtgaaggttt tacccgaacc ggtcacccca
     4321 agcagtacct gagcaggatc gcctctatcc agaccttcct tcagttgcct gatcgcttcc
     4381 ggctggtctc cggtcggaaa aaaatccgag gtcaaattga actgcatatt gtatgatttg
     4441 gtttaatctc ctgagttact ggcattaaac aacatatctt tttcctcctt gctcaggcta
     4501 tcatagcccg aacgggccac tttatcaagg atatcatcga tctttctctg cctttcggct
     4561 ctcctgcggt tgaattcatc atctgtcagt ggtctttcgg tggtttgccg gtaattccct
     4621 gaaacaactt tcatctttct tctcccaaaa atggaagtaa attgtttgaa ccacctcatt
     4681 ttaaacaccc cattgaaatt cattcctttt cttaacgaaa gtatataaat taacccaaaa
     4741 accgctccac caatgtgcgc aatatggcct ccggcattct ccgaccggat catgaaaaaa
     4801 tccaggacaa acagaaacaa ggcaatataa aatatccgta tcctgcctaa aaacaaaaga
     4861 tgaaccgtat aaccgggaac atagacggaa attgcggtga caatcgccat gacggatgcc
     4921 gaagcgccca aagcgactga aactggtaat gattcctgaa agaccggaaa caggttataa
     4981 aaaaggatgt aaagtattcc accggataat cctcccgtta gatagaggaa tagcaactgc
     5041 acgggcttca ggaattcaag gaatattttt ccgaaccaga atagccataa catattaaat
     5101 aacaggtgaa agaaatcgag gtgaaaaaac atataggtga aaagcgtcca gattcgggtg
     5161 atcagaatgc cggtatctgc aggcaaagcc atgacatcta tcagaaattc catcaattcc
     5221 ttgtcgggaa atttgaagag aaaggaaatg acacgaagta cgcccaccaa taaccatatg
     5281 gccgtattga tgatgatcag tcgggataat aaatttcctg atctgaaaaa ccggcctgct
     5341 tcctcaagcg gattcctgta atactgcata gtgggattaa tataaacgac ctttcttctt
     5401 ccagtaaagg atcaggaaga atccaaaaat cataccgccg aggtgggcaa aatgggcaac
     5461 atttgatcct gagccggtaa atccataaaa caattctatc aatccgtaag caatcacgaa
     5521 atatttggct ttgagaggga aaaagaaata gatataaatc agggtattgg gaaataacat
     5581 accaaacgcc aataagattc cgaataccgc gcctgatgcc ccgatgacat ttggtaggtt
     5641 catgtagaac tctctgtagt ccaccaataa attcttcact atataaattg cctgcttatt
     5701 atccgggctg gttcgcagga tttccatcgc accgaagaac ttctgaggat ccgagtagat
     5761 ttccttgaga tatatctgga atagttcggg atcaccggat ataaaatcgc tgactgcctg
     5821 ataagtaggt gccgccatga ttgcattgat atcggataat accgggctca ggtcaaagaa
     5881 ataaaagaca agataatgga tcagggcggc cccgattcct gtaataaaat agtagatcag
     5941 gaaccttttt ggcccccata tattttcaag ggcgttgc
//
LOCUS       DAOQXS010000113         5962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13607_length_5962_cov_4.306247, whole genome shotgun sequence.
ACCESSION   DAOQXS010000113 DAOQXS010000000
VERSION     DAOQXS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13607_length_5962_cov_4.306247"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..491)
                     /locus_tag="PKM34_04505"
     CDS             complement(6..491)
                     /locus_tag="PKM34_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNQ82880.1"
                     /translation="MEEQRIKMIAKTFEGFEEILARELRDLGAEDIDLIKRGIHFSGD
                     KKLLYMANYNCRTALRILVPLSSFRASTDAMLYDGVRAIPWEKFLDFRGKFAIDSVIS
                     YSTFTHSGYVSLKAKDAIADRFRDKFGKRPDVDNNDPDLRVNVHIFREECTLTRRSGS
                     L"
     gene            800..2278
                     /locus_tag="PKM34_04510"
     CDS             800..2278
                     /locus_tag="PKM34_04510"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HNQ82881.1"
                     /translation="MLFRIFLDHQWKEYRRSPVWQRNMVANIFVGFFSFLMIVYLLMI
                     GFFLGPLLSEIMPDSDPVAIINGIILYFLGFDLLIRYLMQALPTFAIESYLHLPVRKS
                     SMVHFVISKSLFHLLNFLPLLVFIPFAARTILPSHGGAASVAWIVSILFLVISNNFLA
                     TYLKRILVSRPVITLLAGIVMIGLAAMDIYGLIRLSDISASAMGAVLKNPFLAILPVI
                     LAFLMYALNYFYLKSKVYPDEVIRHKTIQVQDIPRIKYLNSMGLTGDLIMLDIKLWWR
                     HKRTKTMLYMFPIFVLYGLFFYPNPIYRNDFSWLIFVGTFMTGGMTMNYLNYAFGYES
                     NHFDGLLTSRVNMDLYIRAKLTIGMLITTFCFIITIPYFLFSMDILLINFVAFLFNLG
                     FLSYLLLYMATFNKKRMDLAKGSSFNYQGVGLMNWLVLIPAFVLPLIIFAPFNLTGNR
                     YTGLAAIGLLGILGLISRRFWVKMIEKGFYARKYTMAEGFRE"
     gene            2491..3186
                     /locus_tag="PKM34_04515"
     CDS             2491..3186
                     /locus_tag="PKM34_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82882.1"
                     /translation="MIGVSNLSKVYSGVTVLNIDSLEIKAGESFGLVGNNGAGKTTLF
                     RLILDLIRPQTGFVTSKEQDVSRSENWKLYTGSYLDRNFLIDFLTPEEYFDFNARIHG
                     MSPEDLQGFLESMTEFFNDEILGKKKFIRDLSKGNQQKIGVAAAFMADPEVIILDEPF
                     NGLDPSSQIRLINMLNRLKENKRTTLLISSHDLNHVTEVCSRIAILEKGIIVKDLQTE
                     ENTLKELRNYFSV"
     gene            3208..5751
                     /locus_tag="PKM34_04520"
     CDS             3208..5751
                     /locus_tag="PKM34_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNQ82883.1"
                     /translation="MRNILLKSLLLISFLLAFQPDAMPQHPYSVSGRVIDAETREPLA
                     FVNIIQTGTKTGISTDIDGKFTLKSDRPIDAIWLTYVGYFPLQYSIPYGEVDKILIKL
                     QRKTFDLNEVVILPTANPAHRIIDSVLANRYRNDPEKLASFSYTSYEKTILTAKLDTM
                     MRVGKDSTGNWYSDTLIMEPDTAVMMTDTTLSDTALAEMKEFFDKQDIAIMESVVERK
                     FLAPDRNYENVKANRVSGFQDPIFVFLVSQIQSTSFYKEFFNILNKSYINPISEGSKN
                     KYIFIIEDTLYTERSDTVFIISYRPKPNTNFDGMEGVLYINSYKWAIQNAIARPARQE
                     GFSIKIQQKYDLIDDTQWFPVQLNTDVIFARMQVAAGEARALLVGIGKSYIRDIVINP
                     ELVRRQFATLGVDVDPDSHEKPDDFWNAYRIDTLTDRDRRTYEFLDSIGKEANFDRIA
                     QSFETLMSGRIPWKIIDIDLDRIIKYNDYQGIYLGLGAHTNDKVSRYFKIGGYWGYGF
                     KDKSAKYGGDVSVVLNRRHEVTWGAQYTYDLAESGGVRFFDDTDNFLTGENWRNFLVN
                     RENPVRNLGTTLGFRLLRDFKFGLGFRMITKEASDGYRYRIAEGEGTTVSLDEFRFTQ
                     VTAGFRFAFREQFIVTKRARMSLGTKYPVLWLEYTRGINGLLDGEFAYNRLDAKIEKS
                     FYTKYIGKTSLLLRAGYIDRGIPATDLFNGNGSYRFLTIFADNSFGTMRMNEFLSDRY
                     AALYFTHNFGKLLKRWEKFQPEFVITTNAAFGWLKHNDNHLGINYKTMERGYYESGFL
                     VNNLLNLQVYSLGIGASYRWGPYSYPLFKDNIALKISIMFPMGSLNVGN"
BASE COUNT         1461 a         1471 c         1492 g         1538 t
ORIGIN      
        1 gtcgattaca acgatcccga tctgcgggtc aatgtgcatt cttcccggaa aatatgcaca
       61 ttgacccgca gatcgggatc gttgttatcg acatcgggcc gtttgccgaa tttgtcgcgg
      121 aaccggtcgg caatcgcatc tttcgctttc agcgacacat aacccgaatg tgtgaatgtg
      181 gagtagctga tcacgctgtc gatggcgaat ttcccccgga aatcgaggaa cttttcccac
      241 gggatggccc tgacgccgtc gtaaagcatg gcatcagtgg atgccctgaa gcttgaaaga
      301 ggtaccagga tgcggagcgc cgtccggcag ttgtagttgg ccatgtacag caacttctta
      361 tcaccgctga aatgaattcc ccgtttgatc aggtcaatat cttccgcgcc aaggtccctc
      421 agctccctcg ccagtatctc ctcaaaacct tcaaaggtct tggcaatcat ttttatgcgt
      481 tgttcttcca tgccggatcg ggtttggtag tccgacgggt aaatccgtcg gtgagttcat
      541 ttagtgaggt tgttttaaaa gtccatattt tcactttttt tttggtagtc cgacgactga
      601 agccgtcggt tccaaccctc ataaaacatt cggaaccaac ggctttagtc gttggagcac
      661 gaactgtttt caggcaatca aatcgaaata tcagactttt agacagcccc tgaattttgt
      721 agtccgacgg gtaaatccgc gggttccaaa gttagtgtaa aaatattttt ttactttgca
      781 ggaagaaaac tttcctgtta tgctgttcag aatattcctg gatcaccagt ggaaagaata
      841 ccggcggtcg cctgtctggc aacggaacat ggtggcgaac atcttcgtcg ggttcttctc
      901 cttcctcatg atcgtctacc tgctgatgat cggttttttc ctcgggcctt tgctttctga
      961 aataatgccc gacagcgatc ctgtcgccat aataaacgga atcatcctgt atttcctggg
     1021 tttcgacctg ctgatccgct acctgatgca ggcgcttcca acgtttgcca tcgaatcgta
     1081 cctccatctc ccggtcagga aaagctcgat ggtgcatttc gttatttcca aatcgctgtt
     1141 ccacctgctt aattttctgc cgctgctggt atttatcccg tttgcagcca ggaccatcct
     1201 cccgtcccac ggcggagctg catctgtggc atggatcgtt tccatccttt tcctggtcat
     1261 cagcaataac tttctggcaa cttacctgaa gcggatcctc gtctcccgcc cggtgatcac
     1321 tttgctcgcc gggatagtta tgattggcct tgcggcgatg gatatatacg gtctgatccg
     1381 gttatcggat atttcagctt ccgccatggg cgccgtcctg aagaatccct tcctggccat
     1441 tcttccggta attctggcct ttctaatgta tgcgctgaat tatttctacc tgaagtcgaa
     1501 agtctatccc gatgaggtga tccggcataa aaccatccag gtacaggata ttccgcgtat
     1561 caagtatctg aattcgatgg gattgacagg cgacctgatc atgctcgaca tcaaattgtg
     1621 gtggaggcat aagcggacta agacgatgct gtatatgttt cccattttcg tcctgtacgg
     1681 gttgtttttt tatccgaacc cgatttacag gaatgatttc tcctggctga tctttgtcgg
     1741 gacctttatg acgggaggga tgacgatgaa ttacctgaat tacgctttcg gctacgagag
     1801 caatcatttc gacgggcttc tgaccagccg cgttaacatg gatctttaca tccgggccaa
     1861 gctcacgatc gggatgctga tcactacctt ttgtttcatc atcaccattc cgtactttct
     1921 gttttcgatg gatatcctgc ttatcaactt cgtggcattc ctgtttaacc tgggtttctt
     1981 atcatacctt ttattatata tggcaacttt caataagaag aggatggacc tggcgaaggg
     2041 atcgagcttc aactaccagg gcgtcggcct gatgaactgg ctggttctga ttccggcatt
     2101 tgtccttccg ttgatcatct tcgccccgtt caaccttacc gggaacaggt acacgggcct
     2161 tgccgccatc ggtttgctgg gcattctggg tttgatatcg aggaggttct gggtaaagat
     2221 gattgaaaag ggattttatg caagaaaata tacaatggct gaggggttta gggagtaagg
     2281 atgaatatca accaataaat gctccgacga ctaaagccgt cggttgatga taatgtgctc
     2341 cgacgactaa agccgtcggt tgctgataat gtgctccgac gactaaagcc gtcggttctg
     2401 gggaggttac cttgaaccga tgggtttacc cgtcggactg caatcttaaa aaataccttc
     2461 ggactgcaat cttaaaaaaa tagtaatcat atgatcggag tatccaatct gtcaaaagtc
     2521 tattcaggcg tcaccgtcct gaatatcgac tcgctggaga tcaaagccgg tgaaagtttc
     2581 ggacttgtcg ggaataacgg tgcgggtaaa acaacccttt tccgcctgat cctcgacctt
     2641 atccggcctc agaccggttt tgtcacttcc aaagaacagg atgtgtcgcg gtcggagaac
     2701 tggaaacttt acaccggttc ttacctcgac cggaatttcc tgatcgattt ccttacgccg
     2761 gaagagtatt ttgatttcaa tgcccgtatc cacgggatgt cgccggaaga tctgcaaggt
     2821 ttcctggaat cgatgacgga gttttttaat gacgagatcc tcgggaaaaa gaaattcatc
     2881 cgcgaccttt ccaaaggaaa ccagcaaaag atcggcgtgg ccgcagcgtt tatggccgat
     2941 ccggaagtca tcatcctcga tgaacctttc aacggccttg atccgtcatc acagataagg
     3001 ctgatcaata tgctgaacag gttgaaggaa aacaaaagga caacactgct catctccagc
     3061 catgatctca accacgtgac cgaggtctgc agccggatcg ccatcctgga aaaggggatc
     3121 atcgtgaaag acctgcagac cgaagagaac acccttaaag agcttcggaa ttatttctcg
     3181 gtctgaggaa ttgcctattt ttgcagcatg cgcaatattt tactgaaatc actgctgctt
     3241 atctcttttt tgctggcatt ccagccggat gccatgccac agcaccctta ctccgtttcc
     3301 ggccgggtga ttgacgccga aacccgcgaa ccgctggctt ttgtcaatat catccagacc
     3361 ggaaccaaaa ccggtatttc caccgatatc gacggcaaat tcaccctgaa atccgaccgc
     3421 ccgatcgatg cgatatggct gacgtatgtc ggttatttcc cgctacagta ttccatcccc
     3481 tacggggaag tggataagat tttgattaaa ttgcagcgta agactttcga tctcaatgaa
     3541 gtcgttattc tgcccacggc taatccggcg caccggatca tcgacagcgt gctggccaac
     3601 cgttaccgga acgatcccga aaaactggca tccttttctt acacatctta tgaaaaaacc
     3661 atcctgacag caaaactgga tacgatgatg cgggtgggca aagacagcac cggcaactgg
     3721 tactccgata cgctgatcat ggagcccgac acggcagtga tgatgaccga cacgactctt
     3781 tctgacacgg cgctggccga gatgaaggag tttttcgaca aacaggatat tgccatcatg
     3841 gaatcggtgg tggaacgcaa attcttggca cccgaccgca actatgagaa tgttaaggcg
     3901 aaccgggttt ccggtttcca ggacccgatc ttcgttttcc tggtatccca gatacagtcg
     3961 acatcattct acaaagaatt ctttaatata ctgaataaga gctatataaa tccgatcagt
     4021 gagggcagta agaataaata catcttcatt attgaagata cactctatac cgaaagatcg
     4081 gatacggtct tcatcatttc ttatcgtcct aaacccaata cgaatttcga cgggatggaa
     4141 ggggtgctgt acatcaattc atacaagtgg gccatacaga acgctattgc acgaccggcg
     4201 aggcaggagg gcttcagtat caagatccag cagaagtacg acctgatcga cgatacgcaa
     4261 tggttcccgg tgcagttgaa caccgatgtg atctttgccc ggatgcaggt tgcggccggc
     4321 gaagcacggg cactgttggt gggtatcggc aagagctaca tccgcgacat tgtgatcaat
     4381 cccgagctgg tcaggcggca gtttgccacg ctgggcgtcg atgtcgaccc cgattcccac
     4441 gaaaaaccgg atgatttctg gaatgcatac cggatcgata cgctgaccga ccgtgaccgg
     4501 cggacctatg aattcctcga cagtattggc aaagaggcca atttcgaccg gattgcgcag
     4561 agtttcgaaa cgctgatgag cggacgtata ccctggaaga tcatcgacat cgacctcgac
     4621 cggatcatca aatacaacga ttaccagggg atttacctgg ggctgggtgc gcacaccaac
     4681 gataaagtga gccgctattt taagatcgga gggtattggg ggtacggttt caaagataaa
     4741 tcggccaaat acggaggcga tgtgagcgtg gtgctgaacc gccggcatga ggtcacctgg
     4801 ggggcgcaat atacctatga ccttgcagag agcgggggag tgaggttttt tgatgatacc
     4861 gacaatttcc tgactggtga gaactggcgg aacttccttg tcaaccgcga gaacccggtc
     4921 agaaacctgg gaacgacgct gggattcaga ttattgagag acttcaaatt cggcctgggt
     4981 ttcaggatga tcacgaagga ggcatccgac gggtaccgct accggattgc cgagggagag
     5041 gggacaaccg tttcgctcga tgaattccgg tttacgcagg tgacggcagg gttccggttc
     5101 gccttccggg agcagttcat cgtgaccaag cgggcgcgga tgtcgctcgg aacaaagtac
     5161 ccggtgctgt ggctggagta cacccgcggg atcaacgggc tactcgacgg cgagtttgca
     5221 tacaaccggc tcgacgcgaa gatcgagaaa tcgttctata caaaatatat aggaaagacc
     5281 tcgctgctgc taagggcggg gtacatcgac cgtggcattc cggctaccga tcttttcaac
     5341 ggtaacggca gctaccggtt cctgaccatt ttcgccgaca acagtttcgg gacgatgcgg
     5401 atgaacgagt tcctgtcgga tcgttatgcc gcattgtatt ttacccataa tttcggaaaa
     5461 ctgctgaagc gctgggaaaa gttccagccc gaatttgtca tcaccaccaa tgcggcgttc
     5521 ggctggctga agcataatga taaccatctg gggatcaatt ataagacgat ggaaagaggt
     5581 tattacgaga gcggattcct ggtgaacaac ctgctgaacc tgcaggtcta ttcactcggc
     5641 attggcgcca gctaccgctg gggaccctac agttatcctt tgtttaagga taatatcgca
     5701 ctgaagatat cgatcatgtt cccgatgggg agcttgaatg tcgggaatta gagttgtagg
     5761 ttataagggt ttaaggtttt atgggtttta agggtttaag gattggtgaa gtgttgacta
     5821 cgaatcgtat aatcacctgc ttaccatgaa ctttcctcct tttacccgcc gcccgtcctg
     5881 caaaattgag ataaagtaaa tgccagtcgg caggcccgaa acatcaaaag accaacgcac
     5941 ctctcccttc tccgccaact gg
//
LOCUS       DAOQXS010000114         5948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13661_length_5948_cov_3.609028, whole genome shotgun sequence.
ACCESSION   DAOQXS010000114 DAOQXS010000000
VERSION     DAOQXS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_5948_cov_3.609028"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKM34_04525"
     CDS             complement(<1..1042)
                     /locus_tag="PKM34_04525"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNQ82884.1"
                     /translation="MKIKIIAAFALVLLYGINSFAQSYVILAQPAGSKDWGYADLSGN
                     LIIDAKYRKCTSFSEDGYAAIYDGKSKQFYFIDLKGETLQTEITGYGLIDIFLFGIKG
                     FSDGFAAVEVNGKWGFMNTQGKLAIPAEYDKVTVFRSGYASAKKGIHFIVVDQNGNEK
                     SVNIPGITDLNDFSENLAVFKTEGGVGFIDGSGSVAIQPKFDAAGDFSGGLAWAKTSA
                     GKVGYINPTGEWVIQPNFDAGKNLDPSCGLARIKKDDKWGYVNLSGEILYMTDTELWE
                     DFMGGLARGKKNGKFGYFNDKMEWAVEPQFDGARDFKNGYASVRKGSLWGVIDANGNW
                     VIEPRFEDIKDVE"
     gene            complement(1117..3531)
                     /locus_tag="PKM34_04530"
     CDS             complement(1117..3531)
                     /locus_tag="PKM34_04530"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNQ82885.1"
                     /translation="MKPLFKALILFAFICLNSLAQEKIVVYQADAGCTPENKTIDISH
                     MKADLRINPYDTLVEGEVEFTFRCINTSADSVVFWAPDIEISDVSISGQKINYSKSGD
                     NLIVRYLDSFNYVKDDDHIIKMKYTSRPKYDLFFIGWNEPAVRDNKQIWAHRPFHWLP
                     YYGDRLTTDVRITFDGKYKVMTNGVRESVRENSDGTKTWHYRMYKEHPFFSTCLVIGD
                     YKFLEDKTKDGLPLELWYYSNQEDHAEPTYRYTPVMFDFFNEEFGLAYPYELYREAPV
                     EDYLYGAMETTTSTVFGDYLAVDERGWDGRNYVNVNAHELAHQWFGNCLSHLRPCDVW
                     LTESFATYYAKLFEKQVFGDDYYQWVRIQEFDETLEASKKDRYAVGHSSGGRARWYPK
                     GSLVLDMMRDVLGDEAFQEAVSHYATTNAFSEVETAEFRAAVYEATGKSLDWFFDEWI
                     MRGGEPHYQVSWAAEKTMDDNIATVVYVKQAHPRDNLTGLFKMPVTFEVYYEDSSFDR
                     KLQWIQEEYSEVRIPNPKNKKVSFVLFDPGRRILKTVSFPKSPHELCDQALHAPLMTD
                     RYDALVSMRNLGLDLKEKTLLEAWNAETFHLTRTEILKQLFAGQSARAEEIYNQAIMD
                     QDPLVRRAALENIGKVPDKAKDETEKLLSDISYIVVEKTLEVLCRSFPQDITRYLEAT
                     KNETGWRGRNIRMKWLEIAMQAEQREYTREITDYAGPQYEFETRINAFNLLRELNYLD
                     KRGAAFLVDGCLYWNYKVSNAAKDVLGYFYQQNRYKTLVNGILYDGSLPDARVKKIKE
                     ILGAIK"
     gene            3628..4974
                     /locus_tag="PKM34_04535"
     CDS             3628..4974
                     /locus_tag="PKM34_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82886.1"
                     /translation="MKKSIYLFIFAAFLMSITLNAQVKTMHLPEVLPSVTSDQVKAFG
                     EASFDSKSKVVVLDFEGIGNLDEITQFYNGGASSAGFSGKNYGISFGKSALALVDAQH
                     GGTGNFNTKPMPNTVMFFLKGNTTVINVHQGFKGPFAFDYTSSVLGTVAFYTEPDGRG
                     TLISTEQFQPLATGSKGIDNGYFDNWKSYTASFSGTAKSVVITCAADQCAFDNILIGD
                     ETYKNSLPGGGPVVTGSKTGDTGGAFQAASTGSYYFPGTKGKLFVAGYSGLDISAGTR
                     KSKYNGEIDDGSKYSLFNIYFDPKAGYFIIDNLVAGLFIDLNYYSSKSKDEFGYSNKG
                     TTLEIGPFARYYFPICDKLVPYGEAQIGVGVDNSSSRSSSSSEWYKSKSSVFAYQLGG
                     GATYFFNSNVGADAFIGFSHYSNKYKDSESGERSPGSKYIYNELVLQLGIVIVLGY"
     gene            complement(4980..>5948)
                     /locus_tag="PKM34_04540"
     CDS             complement(4980..>5948)
                     /locus_tag="PKM34_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ82887.1"
                     /translation="GIPVNKMLQSERVKLLHMEEELHKRVIGQDEAIGALADAIRRSR
                     AGLQDAKRPVGSFIFLGTTGVGKTELAKALAEYLFNDENAMVRIDMSEYQERHTVSRL
                     VGAPPGYVGYEESGQLTEAVRRKPYSVILLDEIEKAHQDVFNILLQVLDDGRLTDNKG
                     RIVNFKNTIIIMTSNLGSQVIQERFAGLTVSNKELIVSQTREEVFNLLRLTIRPEFLN
                     RIDDIIMFKPLNENEIRKVVLLQFNQLQAKLAKNNLDIRMTDGAADWVARQGFDPQYG
                     ARPVKRIIQRTILNEFSKMILSAEITSEKPVLIDCSDDRIIFKNVP"
BASE COUNT         1525 a         1494 c         1249 g         1680 t
ORIGIN      
        1 tttcaacatc ttttatatct tcaaacctcg gttcgatcac ccagtttccg ttggcatcga
       61 taacgcccca taaggaacct ttgcgcacgg aagcgtagcc gttcttaaaa tccctggcgc
      121 cgtcgaactg aggttcaaca gcccattcca ttttatcgtt aaagtagccg aatttgccat
      181 ttttctttcc cctggccaat cctcccatga aatcttccca aagctcggta tcggtcatat
      241 agaggatttc gcccgacagg ttgacatatc cccatttgtc atcctttttg atcctggcca
      301 gtccgcacga cggatccagg ttctttccgg catcaaagtt gggctggatc acccattccc
      361 ccgtcgggtt gatatacccg accttgcctg ccgaagtttt cgcccaggct aaaccgccgg
      421 aaaaatcgcc tgccgcatca aacttgggct gtatcgccac gctcccgctt ccatcgataa
      481 acccgacacc gccttctgtt ttgaatacgg ccaggttctc tgaaaaatca ttcaaatcgg
      541 ttattcccgg gatattgacc gatttctcat ttccattctg atcaacaaca ataaaatgaa
      601 tgcctttttt tgccgaagca tagccgctcc ggaaaacagt gactttatca tactccgccg
      661 gaatggcaag ctttccctgg gtgttcatga atccccattt accgttgacc tcaaccgctg
      721 caaatccatc gctgaatcct tttattccga acaagaatat atcgatcagg ccataaccgg
      781 ttatctctgt ctgaagtgtc tcgcctttaa gatcgataaa ataaaattgc ttcgatttac
      841 cgtcgtaaat ggccgcgtaa ccatcctctg aaaagctggt gcatttcctg tactttgcgt
      901 cgatgatcag gttacccgaa agatcggcat aaccccagtc ttttgaaccg gctggctgtg
      961 ccaggattac ataactctgt gcaaatgaat taatcccata aagcaatacg agagcaaaag
     1021 cagcgatgat tttaattttc ataatgataa aagattaata attaactatt tgtgttaaaa
     1081 agcgattggt atttccaaag caaaattaca aacaacttat ttgatcgcac ccaggatctc
     1141 ctttattttt ttcacccggg catccggcag tgatccatcg tataagatac cgttaaccag
     1201 tgtcttatat ctgttctgct gataaaagta acccaaaaca tctttcgcgg cattgcttac
     1261 tttgtaattc caatagaggc acccgtcgac caggaaggcg gctcctcttt tatccaggta
     1321 attcagttct cttagaagat taaacgcatt gatccgggtt tcaaattcgt attgcggccc
     1381 ggcataatct gtaatttcac gggtatattc ccgttgctcc gcctgcatcg ctatttccag
     1441 ccacttcatc cggatgttcc ttcccctcca cccggtttca tttttcgtag cttccaggta
     1501 gcgtgtaata tcctggggaa aagaacggca aagtacttcc agtgtttttt caaccacaat
     1561 ataagaaatg tcggaaagta atttctcggt ctcatcttta gctttatccg gaactttgcc
     1621 gatgttctcc agcgccgcac gcctgaccag cggatcctgg tccataatag cctggttata
     1681 tatttcttcc gccctggcgc tttggccggc aaaaagttgc ttcaggattt ccgtcctggt
     1741 caggtggaaa gtctctgcgt tccaggcttc cagaagcgtc ttttctttaa ggtctaatcc
     1801 caggtttctc atggatacaa gggcatcgta gcggtctgtc atcagcgggg catgcaatgc
     1861 ctgatcacac aactcatgtg gagactttgg aaatgaaact gtctttagta tccttcttcc
     1921 cggatcaaaa agcacaaaag agaccttttt attctttgga ttcggtatcc tgacttcgct
     1981 gtactcctcc tggatccact ggagcttcct gtcgaaactg ctatcctcat aataaacttc
     2041 aaaggtgacc ggcattttga acagcccggt cagattatcc ctgggatggg cctgctttac
     2101 atatacgacc gtcgcgatgt tatcgtccat cgtcttctct gcagcccagc tcacctggta
     2161 gtgtggttcc ccgcccctca tgatccattc atcgaaaaac cagtcgagcg atttcccggt
     2221 ggcttcataa acagccgccc tgaattccgc tgtctctacc tcagaaaaag cattcgttgt
     2281 tgcataatgg cttaccgctt cttgaaaggc ctcatcgcca agaacatccc gcatcatatc
     2341 cagcaccagc gatcctttcg gataccatcg ggccctccct ccgctgctgt ggccaaccgc
     2401 atacctgtcc ttttttgaag cttctagcgt ttcatcaaat tcctggatcc tcacccactg
     2461 gtaataatca tctccaaaaa cctgtttttc aaatagcttt gcatagtaag tcgcaaaact
     2521 ttccgtcagc cagacatcac atggccttaa atgcgacagg cagttcccga accactggtg
     2581 ggccagttca tgggcattga cattcacata gttacgaccg tcccagcctc tttcatccac
     2641 ggccaggtaa tctccaaaaa ccgttgaagt ggtcgtttcc attgcaccgt aaaggtaatc
     2701 ttcaacaggc gcttcccggt aaagctcata ggggtatgcc agcccgaatt cttcgttaaa
     2761 gaaatcaaac atcacaggtg tgtacctgta agtcggctct gcatggtctt cctggttact
     2821 ataataccag agttccagcg gcaggccgtc tttggtttta tcttccagga atttgtaatc
     2881 gccgataacc aggcatgtcg aaaagaaagg gtgttccttg tacatcctgt aatgccatgt
     2941 ttttgtaccg tcagaatttt ctcttaccga ttctctcacc ccattggtca taaccttgta
     3001 ttttccatca aaagtgattc tcacatcggt tgttagcctg tcgccataat acggtaacca
     3061 gtgaaaagga cggtgagccc agatctgctt gttatccctg accgccggct cattccagcc
     3121 gataaaaaaa agatcgtatt ttggacggga agtatatttc atcttaataa tatggtcatc
     3181 atccttaaca taattaaatg aatctaaata acgcacgatt aaattatcgc ctgattttga
     3241 atagttgatt ttttgaccgg aaatggacac atcgctgatt tcaatatcag gggcccagaa
     3301 aacaacggaa tcggcagaag tgttaataca gcggaatgtg aactccacct ccccttcaac
     3361 cagcgtatca taaggattga tcctcaggtc ggctttcata tggctgatgt caatggtttt
     3421 attttccggt gtgcagccgg catcggcctg gtatacaacg attttctctt gagcaagact
     3481 gttaaggcaa ataaaagcaa ataagatcag ggccttaaat aatggtttca ttgggtgtta
     3541 atttttaccg aaaataaata aaattgcatt ggtcttttat ggattgtagt tatttttgac
     3601 ttggcaatta atctaataca taacaatatg aaaaaatcca tctacttatt catttttgca
     3661 gcattcctaa tgtcgataac attgaatgca caggtaaaga ctatgcatct tccggaggtt
     3721 ctgccctccg tcaccagcga ccaggtgaaa gcattcggcg aagccagctt cgacagcaaa
     3781 tcgaaggtcg tggtcctcga ttttgagggg atcggaaacc tggatgagat cacacagttt
     3841 tataacggcg gggccagttc agcgggattc tcagggaaga attacggcat cagtttcgga
     3901 aaatcggctc tggcactggt cgatgcgcag cacggcggca caggcaactt taataccaaa
     3961 ccgatgccga atacggtcat gttcttcctg aaaggcaata caaccgtgat caatgtacac
     4021 cagggattta aaggaccgtt cgcatttgat tacacatctt ctgttctggg gactgttgct
     4081 ttctacacag aacctgacgg acgcggtacg ctgatctcaa ccgagcagtt tcaacccctg
     4141 gccaccgggt ctaaagggat agacaacggc tatttcgaca attggaagag ctatacggct
     4201 tcattttcag gaacagccaa atcggtcgtg atcacatgtg ctgccgatca atgcgcattc
     4261 gacaatatcc tgatcgggga tgaaacatat aaaaattctt tacccggagg agggccggta
     4321 gttacaggat ccaaaaccgg cgataccggc ggcgcttttc aggcagcatc gacgggaagc
     4381 tattatttcc ccgggacaaa aggcaagctc ttcgtggccg gttacagcgg tctggatatt
     4441 agcgcaggca ccagaaaatc aaaatataac ggggaaatag atgatggctc gaaatattcc
     4501 ctcttcaata tttatttcga tccaaaagcc ggatatttta ttatcgataa cctggtggcc
     4561 gggcttttta tcgatctgaa ctattacagt tcgaagtcaa aagacgaatt tggttatagt
     4621 aataagggca caacactgga aataggacct tttgccaggt attattttcc gatttgtgac
     4681 aagctggtcc cttacggaga agcacagatc ggtgtgggtg tagataatag cagttcgcgc
     4741 agttcctcct cttccgaatg gtacaaatcg aaatcctccg ttttcgctta ccagcttggc
     4801 ggcggggcca cctatttctt taactcaaac gtgggggcag atgcatttat agggttcagc
     4861 cattattcta ataaatacaa agattcggaa tccggggaaa gaagcccggg cagcaaatat
     4921 atttataatg aactggtact gcaactgggt attgtcatcg tgcttggata ttaatccggt
     4981 cagggcacat ttttgaaaat tatccggtca tcggaacaat cgatcaatac cggtttttcg
     5041 gatgttattt cggcactgag gatcattttt gagaattcat tcaggattgt tcgctgaatg
     5101 atccgtttca ccggccttgc accgtattgc ggatcaaatc cctgcctggc cacccaatcc
     5161 gctgcaccat cggtcatcct tatatccaaa ttgtttttcg ccagcttagc ctgcaactgg
     5221 ttgaactgca acaggacaac cttacgtatt tcattctcat tcaatggttt aaacataatg
     5281 atatcgtcga tccggttaag gaactcaggt cggatcgtca agcgaagaag gttgaatacc
     5341 tcttcacggg tctggcttac aattagttct ttatttgaca cggtcaaacc ggcaaaacgt
     5401 tcctggatca cttgtgagcc tagatttgaa gtcatgatta tgatagtgtt tttaaaattc
     5461 actatccggc ctttgttatc agtcagcctt ccgtcgtcaa gaacctgcag caggatatta
     5521 aacacgtcct ggtgcgcttt ttcaatttca tccagaagaa tgacagaata aggcttgcgg
     5581 cgaaccgctt cggttagttg cccgctctct tcatagccca cataccccgg gggtgccccg
     5641 acaagcctgg acacagtatg ccgttcctgg tattccgaca tgtctatcct taccatcgca
     5701 ttttcatcgt tgaaaaggta ttcggccagc gccttggcaa gttctgtttt acccactccc
     5761 gtagtaccca ggaagataaa ggaaccgaca ggccttttgg catcctgcaa accggccctt
     5821 gaacggcgga tcgcatcggc cagagctccg atcgcttcat cctgccctat cacgcgcttg
     5881 tgtaactctt cttccatgtg cagcagtttc acgcgctcac tctgaagcat cttattgacc
     5941 ggtattcc
//
LOCUS       DAOQXS010000115         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13666_length_5947_cov_3.552953, whole genome shotgun sequence.
ACCESSION   DAOQXS010000115 DAOQXS010000000
VERSION     DAOQXS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13666_length_5947_cov_3.552953"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKM34_04545"
     CDS             complement(<1..298)
                     /locus_tag="PKM34_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82888.1"
                     /translation="MITIVETPRDAFQGIKQFIPTERKIRIIDLMMKAGFDTVEAGSY
                     VSEKVIPQMADTTQVLRGLDKKNSRSRVMVLVVNKRGAMRAVRQGTQNCHPPAEN"
     gene            404..757
                     /locus_tag="PKM34_04550"
     CDS             404..757
                     /locus_tag="PKM34_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ82889.1"
                     /translation="MKENIIVIKSFTFSLNVLQLYKELMKQKEFIISRQLLRCATSIG
                     ANIEEAGAASSRKDFALKMSISSKEAREARYWLKLLKEDNFIEGYDFSALMNEVDQII
                     NILTKIVKTAQENSR"
     gene            complement(796..1797)
                     /locus_tag="PKM34_04555"
     CDS             complement(796..1797)
                     /locus_tag="PKM34_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82890.1"
                     /translation="MNFPKKVTIGDITVRDGFQHEEKFIPTEAKLWLAEELLLCGFKH
                     IEVTNYGNPKGMPQFKDADELMKALRTSKKVAHLLGEVKLTTITIREKAIERAIQARK
                     EGYGPDRILLMVSTSESHHRTNSGISIEEYFRMAEKYIPMALDAGLKVNGTVSTIWGC
                     PIEGPTEMKKAIEFARRWFDIGATDVEHADHDGSASPDRVYRYYSMLLDAFGSPDKHI
                     VHFHTTRGWGLANVLAALQAGMTNYESTLGGIGGQPANFVDGVPVPGTGEYYYKDPMI
                     VGLVSTEDMVVMMDEMGIDTGLDIDRLLEAGKMAERIVGRRLRSETIRNGRIPKHMK"
     gene            1995..2897
                     /locus_tag="PKM34_04560"
     CDS             1995..2897
                     /locus_tag="PKM34_04560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ82891.1"
                     /translation="MMDSENKSCPLCHSGTGKDYIRVRDFFLSGESFLLLKCSNCGFV
                     YTAGVPAPNEIGKYYKSDEYVSHSDTRKGVMNRLYHTARSIMLYLKFRMVRNESEGDR
                     LLDIGCGTGYFPAYMQRRGFGVNGVEVDPAAAQFARKTFGLNVATPAEFLNREIQGYF
                     DIITLWHVLEHLDDFDLYIDRMLEYLLPGGLLVIALPNCSSPDARHYKEYWAGYDVPR
                     HLWHFTPSTFKVLADKHRLKIIKMKRLPLDPFYNSMLSEKYRKSRFFMIKGTIIGKLA
                     YLESLFSLKKSSSVVYFLKRKSDS"
     gene            2989..3522
                     /locus_tag="PKM34_04565"
     CDS             2989..3522
                     /locus_tag="PKM34_04565"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNQ82892.1"
                     /translation="MSTTQWIILISGTILLILFSWFVSLREKRYHGIPRFFVFEGLLI
                     LGLLQADTWFHDPLVPRQIISWLLFISSIFYAFYGIMIFIKKGKPGENFENTTRLVTT
                     GLYRFIRHPMYASLLFMGWGMFLKEINMVTALITAGITLAVYLTCRVEEKEMIARFGE
                     EYREYMKRTRLWIPYLI"
     gene            3605..3922
                     /locus_tag="PKM34_04570"
     CDS             3605..3922
                     /locus_tag="PKM34_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNQ82893.1"
                     /translation="MNWIFLIIAGAFEIGWPLGFKLSQTTPYKFGWILFSIAAMGLSG
                     LFLWMAQKTIPMGTAYAVWTGIGAVGAFVTGIILFKDPVTALRIVSVVFIITGIVGLK
                     IAG"
     gene            3936..5288
                     /locus_tag="PKM34_04575"
     CDS             3936..5288
                     /locus_tag="PKM34_04575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HNQ82894.1"
                     /translation="MRTLRIIIFILTLAFTGCQSTVVKTDDQKIADQVKSEFLRSWKA
                     YKTYAWGHDVLLPLSKSHMDWYENSLGISPIDAYSTMKVMGLDEEAAKVQKYVADTIN
                     FDKDQFVKTFEVNIRVLGGLVAMYQFTKDPKVLAKAEDFGKRLLAAYNTGTGIPAYWV
                     NLRTGEIKGDTVNLAEGGSSLIEMGMLSRFTGNPVFFDAAKKASLAIHARRSSLGLVA
                     ENINVQTGQWVDSVSHIGCCIDSYYEYLLKGWILFKDEDLKRAWEESLVAIRKFVAEE
                     PDSSLWYGQVNKETGAIVNRVVTLYDAYFPAVLALSGDLENAARYQDSWHSLWLKYGL
                     EPMVYDYGKKEILHPAYNLNPEIIESAYYLWYFTGEKKYFEMASQYWYDIMAYCRTDV
                     AFTNIKDVRTKEQADHMETFFLAETLKYLYLIFANPPGINPKECVFSTEAHPFRKPAA
                     "
     gene            5397..>5947
                     /locus_tag="PKM34_04580"
     CDS             5397..>5947
                     /locus_tag="PKM34_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNQ82895.1"
                     /translation="MRLIMGFMALLMAGLTGCGPSPQKPSPDAFPGLLDKAKASFSPI
                     TEPVDNPDNPITKEKVILGQTLYFDKRLSKDETLSCNSCHDLQTYGVDNEATSGGVGG
                     QKGERNSPTVLNAAYHFSQFWDGRAKDVEEQAGGPILNPVEMAMPDQEAVLKRLQAVE
                     GYRKLFAAAFPGEEQPVNYINLQ"
BASE COUNT         1532 a         1334 c         1451 g         1630 t
ORIGIN      
        1 tattctccgc aggcggatgg cagttttgtg tcccctgcct gacagccctc atggcgccgc
       61 gtttgttcac gaccagcacc atgacccgag atctgctgtt ttttttatcc agtccgcgca
      121 gaacctgagt ggtatcggcc atctgcggga tcactttttc ggatacgtag ctgccggcct
      181 cgaccgtatc aaaaccggct ttcatcatca ggtcgatgat cctgatcttc ctttccgtgg
      241 gaatgaactg ttttatcccc tggaaagcgt cgcggggtgt ttcgacaatt gttatcaatg
      301 ttgaatgttg aaggttgaat gttgaattcg ggattttttt attaacaacc gggtattttt
      361 agcctaaaaa ccggattaat ggataaaatt aaatcattat cctatgaaag aaaacattat
      421 tgtcattaaa tcgttcactt tttcgctaaa tgtacttcag ctttataaag aattaatgaa
      481 acaaaaggag ttcattattt ccaggcaact tctacgttgc gccacaagca ttggggcaaa
      541 tatcgaagag gcgggtgctg cttccagtcg caaggatttt gcattaaaga tgagtatttc
      601 ttctaaagaa gcaagagaag cgaggtattg gcttaaactt ttaaaggaag ataatttcat
      661 cgagggttat gatttttccg cgctgatgaa tgaagttgat cagattataa atattttaac
      721 gaagatcgtc aaaacagctc aggaaaattc aagataatat tcccgcattc aacattcaac
      781 ctttaacatt caaacttatt tcatgtgctt ggggattctg ccgttgcgta ttgtctccga
      841 ccgcagcctg cggccaacga tcctttccgc cattttaccc gcttcgagca accggtcgat
      901 atccagcccc gtatcgatcc ccatctcatc catcatcacg accatatctt cggtgctgac
      961 gagtccgacg atcattgggt ctttgtagta atattcgccg gttccgggca ccggaacgcc
     1021 atcgacgaaa ttagccggct gcccgccgat ccctcccagg gtcgattcat agttggtcat
     1081 cccggcctgc agcgctgcca gcacattggc cagcccccag ccgcgggtgg tatgaaagtg
     1141 tacgatgtgc ttgtcgggcg agccaaaagc atccagaagc atcgaataat agcggtaaac
     1201 gcgatcgggg gaggcagacc cgtcgtggtc ggcgtgttct acgtcggtgg cgccgatgtc
     1261 gaaccagcgt ctggcaaatt caatagcctt tttcatctca gtcggccctt cgatcgggca
     1321 tccccagatg gtgctgacgg tgccgttcac cttcaaccct gcgtcgaggg ccatcgggat
     1381 atatttttct gccatccgga aatattcctc gattgaaatg cccgagttcg tgcggtgatg
     1441 cgattcgctg gtggaaacca tcaggaggat ccggtcgggg ccgtaaccct cttttcgggc
     1501 ctggatcgct ctttcgatcg ccttctcgcg gatggtgatg gtggtcagtt tgacctcccc
     1561 gagcagatgg gccactttct tgcttgttcg gagtgctttc atcagttcat cggcatcttt
     1621 gaactggggc atccctttcg gatttccgta gttggtcact tcaatatgtt taaagccgca
     1681 gaggagcagc tcttcagcaa gccaaagctt cgcttccgta ggaatgaact tctcttcatg
     1741 ctggaaaccg tcacgtacgg ttatgtcgcc gatagttact tttttaggga aattcatagc
     1801 tgttaaatgt ttgaatttta gtgcttagtg cttaattata aaagggattt atgattaagg
     1861 atttggggtt attgggttcc taatccgggt tctaagatag gaaaaataat gacagataca
     1921 agaaattgca taaaaggttg ctccagggat attcattgtt aactttggtg cgggttgtgt
     1981 tttttattta ttgaatgatg gattcagaga ataaaagctg tccgctttgc cattcaggaa
     2041 caggaaagga ttatatccgt gttagggatt ttttcctttc gggtgagagc tttttgctcc
     2101 tgaagtgcag caattgcggg tttgtttata cggcaggtgt tccggcgcca aatgagatcg
     2161 gaaagtatta caaaagcgat gaatatgttt cccattccga tacccggaag ggagtgatga
     2221 accggctgta ccatacagcc cggtcaatca tgctgtacct gaagttcagg atggtcagaa
     2281 acgagtcaga aggcgaccgg ctgctcgata tcggctgcgg cacgggctat ttcccggctt
     2341 acatgcaacg cagaggcttc ggcgtgaacg gggttgaggt ggatccggcc gctgcacagt
     2401 ttgcccgtaa gacctttggc cttaatgtgg caacaccggc tgagttcctc aaccgtgaaa
     2461 tccagggata tttcgatatt atcaccctct ggcacgtcct ggagcatctc gatgatttcg
     2521 atctgtatat cgaccggatg ctggagtatc tgctgcccgg cgggttactt gtgattgctc
     2581 ttcccaattg cagttcgccg gatgccaggc actacaaaga atactgggcc ggctacgatg
     2641 taccgcgcca tctctggcat tttacgccat caacattcaa agtcctggcc gataagcatc
     2701 gtcttaaaat tattaagatg aaaaggctcc cgctcgatcc gttttataac tcgatgctga
     2761 gcgagaaata ccggaagagc cggtttttta tgatcaaagg gacgatcatc ggcaaactgg
     2821 cttacctgga aagcctgttc agtttgaaaa aatcaagctc agtagtgtat tttttaaaaa
     2881 gaaaatcaga ttcgtgaata ttcgtaatat tcgtgtcatt tgcgttctga aaaagcacac
     2941 ggataacacg aaatcgacga atatcaatag taccaaaaac aagttcctat gtcaacaacc
     3001 caatggatca ttctgatttc aggaaccatc cttctcatcc tgttctcctg gttcgtttcc
     3061 ttacgggaaa agcgttatca tggcattccc cgatttttcg tttttgaagg attgctgatc
     3121 cttggtttgt tgcaggccga tacctggttt catgatccat tagtacccag gcagatcatt
     3181 tcgtggcttt tattcatttc atctatattt tatgcatttt atggcattat gatatttatt
     3241 aaaaagggaa agccgggtga aaatttcgag aacaccaccc ggctggtcac aacgggattg
     3301 taccgcttca tccggcaccc gatgtacgct tcgcttcttt ttatgggctg gggcatgttc
     3361 ctgaaagaga tcaatatggt gactgcttta atcactgccg ggatcacgct ggccgtttac
     3421 ctcacatgca gggttgaaga aaaagagatg attgccaggt tcggggagga atatagggag
     3481 tatatgaaga gaacacgact gtggattccc tatttaatct agaatttaca gtctacagtt
     3541 tacaatcttt aatcctcttt aagaattaag aattaagaat taagaattaa aaattaataa
     3601 ttacatgaac tggatatttc tgatcattgc cggtgctttc gagatcggct ggccacttgg
     3661 cttcaagtta tcgcagacga cgccctataa atttggctgg atattatttt ccatagccgc
     3721 tatgggcttg agcggtctat tcctctggat ggcccagaaa acaatcccga tgggtactgc
     3781 ttatgccgta tggacaggga tcggggccgt cggagctttt gtaaccggta tcatcctgtt
     3841 caaagatcct gtaactgcat tgaggatcgt ttcggtggtt tttatcataa cgggaattgt
     3901 cggattgaaa atagcaggtt aagaatatat taattatgag gactttaaga ataattatat
     3961 tcattctgac tttggctttt accggctgtc agtcaactgt cgtcaaaacc gacgatcaaa
     4021 aaatagcaga ccaggtcaaa tcggagttcc tccggtcgtg gaaagcctat aaaacatatg
     4081 cctggggcca cgacgtgctt ctcccgcttt caaaaagcca catggactgg tatgagaact
     4141 ccctcggtat ttccccgatc gatgcataca gcacgatgaa agtcatgggg cttgatgagg
     4201 aggctgccaa ggttcagaaa tatgtggcag ataccatcaa tttcgacaaa gaccagtttg
     4261 tcaagacttt cgaagtcaat atccgcgttc ttggcggact tgttgccatg taccagttta
     4321 ccaaagatcc gaaggtgctg gcaaaagcgg aagatttcgg gaagaggctg ttagcagcat
     4381 acaataccgg aaccggcatc ccggcatact gggttaacct ccggaccgga gagatcaaag
     4441 gcgatacggt caaccttgcc gaaggcggct cgagcctgat cgagatgggg atgctgagcc
     4501 gttttaccgg aaaccccgtt tttttcgatg cagcaaaaaa agccagcctc gcgattcatg
     4561 cccgcaggtc atcccttgga ctggtcgcag agaatatcaa tgtccaaacc gggcagtggg
     4621 tagatagcgt cagtcatatc gggtgttgca tcgacagcta ctatgagtat cttcttaaag
     4681 gttggatatt gtttaaagat gaggatttga agagagcctg ggaagagagc ctggttgcga
     4741 tccgcaaatt cgttgcggaa gaaccggact cttcactctg gtatgggcag gtaaacaaag
     4801 agaccggagc gatcgtcaac agggtcgtga ccctctacga tgcttacttc cccgctgttt
     4861 tagctctttc cggtgacctg gaaaacgctg cccgctacca ggattcgtgg cacagcctgt
     4921 ggcttaagta tggcctggag cctatggttt atgattacgg gaaaaaagag atccttcatc
     4981 cggcctataa tttgaaccct gaaatcatcg aatcggctta ttatctctgg tattttacgg
     5041 gtgaaaagaa gtattttgag atggcatctc aatattggta tgatataatg gcttattgca
     5101 ggactgacgt ggctttcacg aatataaaag atgtgcgcac caaagaacag gcggatcata
     5161 tggaaacttt ctttttagct gagaccctga agtatttata cctgatcttt gccaatccac
     5221 cggggattaa tccaaaagag tgcgtttttt ccacggaggc gcatccgttc cggaaacctg
     5281 ctgcctgatc cgcatatttt caatacggca ttcggatatt tctacgaaaa aggtatattt
     5341 acttcaaata atatggatca atgtagagtc cttttaaaat attaaaaacc aaatcgatga
     5401 gacttataat gggattcatg gcattgttga tggccggcct gaccggttgc ggcccatcgc
     5461 cacagaaacc ttcacccgat gcctttcccg ggttactgga taaagcaaaa gcttccttca
     5521 gcccgatcac ggaaccggtg gataacccgg ataacccgat cacaaaagaa aaggttattc
     5581 tgggtcagac gttgtatttt gacaaacgct tatcgaaaga tgaaaccctg agctgcaatt
     5641 cctgccacga tctgcagacg tatggagttg ataatgaagc gacatccggt ggcgtcggag
     5701 gccagaaggg tgaacggaac tcaccgacgg tgctgaacgc cgcctaccat ttcagtcaat
     5761 tctgggacgg aagggcgaaa gatgtggaag aacaggccgg tggcccgatc ctgaacccgg
     5821 tagagatggc tatgcccgac caggaagcag tattgaaacg cctccaggct gtggaaggtt
     5881 accggaaatt gtttgctgcc gcgtttcccg gtgaagaaca accggttaac tatatcaacc
     5941 tgcaaaa
//
LOCUS       DAOQXS010000116         5899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13799_length_5899_cov_4.189596, whole genome shotgun sequence.
ACCESSION   DAOQXS010000116 DAOQXS010000000
VERSION     DAOQXS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_5899_cov_4.189596"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKM34_04585"
     CDS             <1..284
                     /locus_tag="PKM34_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ82896.1"
                     /translation="LPAHYHLLMGGFAFGAVFMATDPVSAAQTNTGKWIYGLLIGVFV
                     VLIRVFNPAYPEGMMLAILLMNVFAPLIDYYVVEANIRRRLKRMKPAKA"
     gene            316..1059
                     /gene="nqrC"
                     /locus_tag="PKM34_04590"
     CDS             316..1059
                     /gene="nqrC"
                     /locus_tag="PKM34_04590"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNQ82897.1"
                     /translation="MYSNSYVFRYAAIMVIVVAAALASVAMLLKPAQDRNVTIEKMRN
                     ILAAANIEATPENAIELFDKYVVEEEALAPDGNVVAVFRGQKLEKGNIRPFEINLKEE
                     LYKHSKGQDFIIPLYIAEVNGKRLYIFPMLGRGLWGPVYGNLALEEDFNTVAGSMFGH
                     DKETPGLGAEIELPQFESQFIGKKIFDDNGNFTSIQVLKGASEVLSPEKLIHGVDAIS
                     GGTITSNGVSDMIKTNLANYIEYIKKQKI"
     gene            1063..1713
                     /locus_tag="PKM34_04595"
     CDS             1063..1713
                     /locus_tag="PKM34_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNQ82898.1"
                     /translation="MKTKERESLFSAKNRKLIINPLNADNPITVQVLGICSALAVTAK
                     LKPAFVMALSVVVVTAFANLVISLIRNGIPPRIRIIVQLTVVATLVILVDQLLKAYVY
                     DVSRQLSVFVGLIITNCILMGRLEAFAMANKPWPSFLDGIGNGLGYSWILIALGFVRE
                     LFGSGTLWGYPVLPQALYDLGYKNNGLIILPPMALILVGLIIWIQRSRTPSLIDKS"
     gene            1740..2357
                     /gene="nqrE"
                     /locus_tag="PKM34_04600"
     CDS             1740..2357
                     /gene="nqrE"
                     /locus_tag="PKM34_04600"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNQ82899.1"
                     /translation="MQEYINLFVKSIFIDNMVFAFFLGMCSYLAVSKTVKTSLGLGVA
                     VIFVLTITVPVNFLIQNYLLAPGALSWISPSLAGVDLNFLSFMMFIATIASMVQLVEM
                     VVEKFSPALYNSLGIFLPLIAVNCAILGGSLFMQEREYPTVVHATVFGLGSGIGWAIA
                     IIGIAAIREKMRYSNVPAPLRGLGISFILTGLMALAFMSFLGIKL"
     gene            2390..3631
                     /gene="nqrF"
                     /locus_tag="PKM34_04605"
     CDS             2390..3631
                     /gene="nqrF"
                     /locus_tag="PKM34_04605"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNQ82900.1"
                     /translation="MGLVIGISIVVFLVIILLLVGVLLYARAKLIPQGKVKISINDDK
                     ELEVSPGSSVLSTLANSGIFLPSACGGGGTCAMCKCQVLEGGGSILPTETGYFTRKEQ
                     QNHWRLGCQVKVREDMKLHIDPHIFGIKKWECEVVSNKNVATFIKEFVVKLPEGENLD
                     FKSGGYIQIDVPVCEVDYGKDIFVEEEYRDEWDKFKMWELKMKNPEPVFRAYSMANHP
                     AESNIIMLNIRIATPPFDRKSGGFMNVNPGICSSYVFSRKPGDKVTISGPYGEFFIKD
                     TRREMMFIGGGAGMAPMRSHIFDQFNTKHSDRKATFWYGGRSLRELFYIDEFREIEKN
                     NPNFKFHVALSEPKAEDNWTGYTGFIHQVIIDNYLKQHPEPEEIEYYICGPPMMNAAI
                     FKMLDDYGVPKEMIAFDDFGG"
     gene            3680..4459
                     /locus_tag="PKM34_04610"
     CDS             3680..4459
                     /locus_tag="PKM34_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82901.1"
                     /translation="MKKVLLFTLLSVFGFMTISFAQDVIYKKDGSKEEVKVTLVGEKE
                     IQYKKFSNPDGPVYSLPKHEIVLITYENGEFETIVPKESAGMPVKTDFTVNFAKNIIS
                     YHMFDLVFGDFTFSYERLLNNGMVGFKIPVAMGYYYYSDFGNFNSIIYSGLGVNFYPT
                     GQGRWRYFVGPQVRAGIGEENEWIEYYDDYGNYLYSDDIENRGFYTQFFIDNGVTFLP
                     VRNFGISVIASIGVRYFPEASYDSDALRPDGQFAVNISYRF"
     gene            4467..5468
                     /locus_tag="PKM34_04615"
     CDS             4467..5468
                     /locus_tag="PKM34_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNQ82902.1"
                     /translation="MTRLSLFGLILMLLSVSCFTPDKKVMLRGEAQGTYYAITYFDRT
                     GRNFQPEIDSILRRFDLSVSLWEPGSILSRINNGDSLALPDEWFTDIFNRSVDIAEKT
                     GGAFDFTVGPLVNAWGFGFRNKIRLDSAKVDSLLELVDFRTVGLYEGKIVKRDPRVQF
                     DFNAIAQGYAVDVLGNFLASEGISNFLIDIGGEVLGKGQKPDGSDWLVGIENPSTDSL
                     SERTLNARVKLSGKALATSGSYRKYYEENGVRYSHTIDPKTGYPVRHSLLSVSVLAGD
                     CATADGYATAFMVMGFEKAKDFVENEPELEAYFINASEDGLFETYATKGFRSLLISD"
     gene            5550..5753
                     /locus_tag="PKM34_04620"
     CDS             5550..5753
                     /locus_tag="PKM34_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82903.1"
                     /translation="MLNVILITIILVGLAFAGFAIKILLTKKGEFKKSCSSVDPVTGE
                     RYGCSCGSGDGGETCSNRQGSGE"
BASE COUNT         1503 a         1353 c         1486 g         1557 t
ORIGIN      
        1 ccctcccggc ccattatcac ctccttatgg gcggatttgc ctttggtgcg gtgttcatgg
       61 ctacagatcc ggtaagtgca gcccagacca ataccgggaa atggatttat ggcctgctta
      121 tcggggtctt tgtcgtgctg atccgggtat tcaacccggc ctaccccgaa gggatgatgc
      181 tggccatcct cctgatgaat gtctttgccc cgctgatcga ctactatgtg gtggaagcca
      241 atatccggag gaggctgaaa cgtatgaaac cggctaaagc atgatttaat ttgaaacgga
      301 ataaacaact acgatatgta cagtaattca tatgtgttcc ggtatgccgc catcatggtg
      361 atcgtggtgg ctgctgcgct cgcttcggtg gccatgctgc tgaagccggc acaggaccgc
      421 aacgtgacca tcgaaaagat gcgaaatatc ctggctgccg ccaatatcga agccactccc
      481 gaaaatgcga tcgaactgtt cgataagtat gttgtcgaag aggaagcgct cgctcccgat
      541 ggcaatgtgg tggccgtctt cagaggacag aaactcgaaa aaggaaatat ccgcccgttt
      601 gagatcaatc tgaaagaaga gctctacaaa cattccaaag ggcaggattt tattattcca
      661 ctctatattg ccgaagtgaa cgggaaacgg ttgtatattt tcccgatgct tggccgcgga
      721 ctgtgggggc cggtatatgg aaacctggcg ctggaagagg atttcaacac ggttgccggc
      781 tcgatgttcg gacatgataa agaaaccccc ggacttggcg cggagatcga attgccacaa
      841 ttcgaatcgc agtttatcgg caagaaaatt ttcgacgaca atggcaactt cacctctatc
      901 caggtactga aaggcgcttc cgaagtcctt tcgccggaaa agttaattca cggcgtggat
      961 gcgatctccg gtggcacgat caccagcaac ggggtttcgg atatgatcaa aaccaacctg
     1021 gctaattata ttgaatatat taaaaagcaa aaaatataac cgatgaagac aaaggaacga
     1081 gaatcccttt tctctgcaaa gaaccggaag ctgatcatta atccgctgaa tgcggataac
     1141 cctatcacgg tacaggttct gggtatctgt tcagcgctgg ctgttacggc aaaactgaaa
     1201 ccggcattcg tcatggcgct gtcggtagtg gttgtcacgg cctttgccaa cctggtcata
     1261 tccctgattc ggaacgggat accgccacgg atcaggatca tcgtccagct gacggttgtc
     1321 gccaccctcg tcatcctggt cgaccagctc ctgaaagcct atgtttacga cgtcagccgg
     1381 caattgtcgg tttttgtcgg gctgattatc accaactgta tcctgatggg ccgcctcgaa
     1441 gcctttgcca tggccaataa accctggcct tctttcctcg acggtatcgg taacgggttg
     1501 ggctatagct ggatcctgat tgcgctggga tttgtcaggg agttgttcgg ctcaggcacg
     1561 ctgtggggat acccggtgct gccgcaggct ttatatgacc tgggatacaa aaacaacgga
     1621 ctgatcatcc tgccgccgat ggccctcatc ctggtaggcc ttatcatctg gattcaaaga
     1681 tctcgaaccc cttcactgat agataaaagc tgatttcatc atcctaaaac cgaactgtca
     1741 tgcaagaata tataaacctg ttcgtcaagt cgatctttat cgacaacatg gtcttcgcct
     1801 tcttcctggg catgtgctcc tacctggctg tgtcgaaaac ggtgaaaact tcactgggcc
     1861 tgggggtagc agtcattttt gtgctgacca ttaccgttcc ggtcaacttt ctcattcaga
     1921 actatctact ggcaccggga gcacttagct ggatttcccc gtcgctggcg ggtgtagatt
     1981 tgaattttct aagttttatg atgtttatcg cgacgattgc ttccatggtt caactggtcg
     2041 aaatggtggt ggagaagttt tcgcccgccc tttacaattc tctggggatt tttcttccgc
     2101 ttattgcggt gaactgtgcg atcctgggcg gttcattgtt tatgcaggag agggaatacc
     2161 cgacagttgt tcatgccaca gttttcggcc tgggatcagg catcggctgg gccattgcca
     2221 ttattggaat tgcagcgatc cgtgaaaaaa tgcggtattc caatgtcccg gcaccactcc
     2281 ggggtctcgg gatatcattc atccttaccg gccttatggc ccttgcattt atgtcattct
     2341 taggtatcaa actttaagtt aaatcatctt caagatattg attatgaata ttggattggt
     2401 cataggcata agtatcgtag tcttcctggt cattattctt ctattggtcg gggtattgct
     2461 ttatgcgcgc gcaaaactga ttccccaggg gaaagtgaag atttcaataa atgatgataa
     2521 ggaactggag gtctcaccgg gatcttccgt gctgtcgacc ctggctaaca gcgggatttt
     2581 tcttccttca gcttgtggcg gaggcggcac ctgtgccatg tgcaaatgcc aggtgctcga
     2641 aggcggtggc agtatcctgc ctaccgaaac aggatatttc acccggaaag aacaacagaa
     2701 ccactggcgc ctgggctgcc aggtgaaggt cagggaagat atgaaactcc atatcgatcc
     2761 gcatatcttc ggcattaaaa agtgggaatg tgaggtcgtc tccaataaga atgtggcaac
     2821 tttcatcaaa gaattcgtgg tgaaacttcc cgaaggcgag aacctggatt tcaaatcggg
     2881 tggctatatc cagatcgatg ttccggtttg tgaagtggac tatggaaagg atatttttgt
     2941 cgaggaagaa taccgggatg aatgggataa atttaagatg tgggaactga agatgaaaaa
     3001 cccggaaccg gtgttcaggg cctattccat ggccaaccac ccggcagaaa gcaacatcat
     3061 catgctcaac atccgcatcg ctactcctcc tttcgaccgg aaatcgggag gtttcatgaa
     3121 tgttaatccg ggaatttgct cctcctatgt attctcaagg aaaccgggcg ataaagtgac
     3181 tatatccggt ccttacggtg aatttttcat caaagatacc aggcgcgaaa tgatgttcat
     3241 cggggggggc gccggaatgg ccccgatgcg ttcccatatt tttgaccagt tcaacacaaa
     3301 acacagcgac cggaaagcta cattctggta cggcgggcgg tcgttgcgcg aacttttcta
     3361 tattgatgaa ttccgcgaga ttgaaaagaa caacccgaat tttaaatttc atgtcgccct
     3421 ctcggaaccg aaagccgaag ataattggac cggctatacc ggctttattc accaggtgat
     3481 catcgataat tacctgaagc aacaccccga accggaggag attgaatatt acatctgcgg
     3541 accgcccatg atgaacgccg ccatctttaa aatgctcgac gattatggtg ttcccaaaga
     3601 aatgatcgct ttcgacgatt tcggaggata aatgtcatat tgcggcatta tctttgcgta
     3661 ctgaaaccaa actaaaatta tgaaaaaggt acttttattt accctgttgt ctgtttttgg
     3721 ctttatgacc atttcttttg cccaggacgt catttataaa aaagatggtt ccaaagaaga
     3781 ggtgaaagtt acccttgtcg gggagaagga gatccaatac aagaaattca gcaatccgga
     3841 cggacctgtt tacagcctgc ccaaacatga gattgtcctg ataacttatg aaaatggtga
     3901 atttgaaacc attgtcccaa aggagtccgc cggaatgcct gtaaaaacag attttacggt
     3961 gaattttgca aagaacatca tctcctacca tatgttcgac ctggtcttcg gtgattttac
     4021 gttttcttat gagcggttgc tgaacaacgg aatggttggt ttcaagattc cggtggcgat
     4081 gggatattat tattacagtg atttcggcaa ctttaacagt atcatttact ccggcctggg
     4141 ggtcaatttc tatccgacgg ggcaggggag atggcgctat tttgtagggc cgcaggtcag
     4201 ggccgggatc ggtgaagaaa atgagtggat cgaatattat gacgattatg gcaattacct
     4261 ttatagtgat gacatcgaga acaggggttt ctatacccag ttttttatcg ataatggggt
     4321 aaccttcctg ccggtcagaa atttcggtat ctcggtgatt gccagcatcg gcgtcagata
     4381 tttccctgaa gcgtcatacg acagcgatgc gctgagaccc gacggccagt ttgcagtgaa
     4441 tatcagttac cgcttttaaa aatcccatga caagattgtc cctttttggg ttgattctca
     4501 tgctattatc cgtttcctgc tttaccccgg ataagaaagt catgctgcgg ggagaagcac
     4561 agggaacata ttacgcaatt acgtactttg accggaccgg gagaaatttc cagccggaga
     4621 tcgattccat ccttcgcaga tttgatcttt ctgtctcttt gtgggagccc ggttcaatcc
     4681 tttccaggat caataacggc gattctttgg cattaccgga tgaatggttc accgatatct
     4741 tcaaccgctc agtcgatata gccgaaaaaa ccggcggagc attcgatttc accgtcgggc
     4801 cgctggtcaa tgcctgggga ttcgggttca ggaacaagat caggctcgat tcggcaaaag
     4861 tcgatagcct cctggagttg gtcgacttta ggactgtagg actatatgaa ggtaagatag
     4921 taaaacggga tccgcgggtg cagtttgatt ttaacgctat cgcgcaagga tatgccgttg
     4981 atgtcctggg gaattttctc gcttcggagg gaatttcgaa tttcctgatc gacattggag
     5041 gagaggtgct gggaaaaggg cagaagcccg atggcagcga ctggctggtg ggcatcgaaa
     5101 atccatccac cgacagcctg tcggaacgta ctcttaacgc acgggtgaaa ctgtcgggca
     5161 aagctttggc gacttcgggc agctacagga aatactacga ggagaacggc gtccgctatt
     5221 cgcacaccat tgatccgaaa accggttatc ccgtgagaca ttcgttactt agcgtatcgg
     5281 tactggccgg cgattgcgct accgccgacg gctatgcgac agccttcatg gtgatgggtt
     5341 ttgaaaaggc gaaagatttc gtagaaaacg agccggaact ggaagcttat tttattaatg
     5401 cctcagaaga tggacttttt gagacttatg caacaaaagg gttcaggagt ttgttaataa
     5461 gtgactgaag caatggttaa aaaggttcca gagttccaga gttccagagt tccaaatttc
     5521 aaatttcaaa tttctaattt ccattttgaa tgctcaacgt aatcctaatc acgataatcc
     5581 ttgtcggtct tgcctttgcc ggttttgcca ttaaaattct gctgacgaaa aagggagagt
     5641 tcaaaaaaag ttgttccagt gtcgatcctg tcactggtga aagatacggt tgttcctgcg
     5701 ggagtggcga tgggggagag acgtgcagca acaggcaagg ctcgggtgaa tgagttatgt
     5761 ttatgcgatc acctcgccac aacaaactta ccactcccca aaaaatcgcc cccacttttc
     5821 agaaccgcga tatacacccc cggaggataa ctttctacat tgatctgtac ctggtcctgc
     5881 ttgccaggaa cctttatct
//
LOCUS       DAOQXS010000117         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13849_length_5883_cov_3.864276, whole genome shotgun sequence.
ACCESSION   DAOQXS010000117 DAOQXS010000000
VERSION     DAOQXS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13849_length_5883_cov_3.864276"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKM34_04625"
     CDS             complement(<1..254)
                     /locus_tag="PKM34_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ82904.1"
                     /translation="MKVFKFGGGVLNSAGAVGRIPEIIRRYPGIPLVIVVSAFGKTTN
                     ALEELIASTISADGRSGRLFRQIKEYHDEITRQLFPNDSK"
     gene            324..3686
                     /gene="mfd"
                     /locus_tag="PKM34_04630"
     CDS             324..3686
                     /gene="mfd"
                     /locus_tag="PKM34_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNQ82905.1"
                     /translation="MKTEELIRLYAAAPQVQAMAGILKNQTSPRIFLQGLAGSSPALI
                     KAAVFHHLQRNHLVILNDPESAAYLYNDLETLFGEKGQPYEHKNTLFFPTVYKRHFEF
                     DKPDRTNMLMRSEVLSRLVSTHKKTLIVSYAEALSEKVVRKKVLTKNTLKLRAGEKVS
                     LDFVADMLIEYGFDREDFVYEPGQFSIRGGILDVFSFAHDNPFRIEFFGEEVESIRTF
                     DPASQLSIDKMDRITILPDLSERLEVESRESFLSFLPPGAMIWVDDLAGLQDLLDVYT
                     DRLQKLLDNDRDGLYPKNLLGHFTGKDDFLKELLGHAIVESGSRPVLKPDLAPEFDIR
                     PQPTFNKNFDLLTADLAVNTKAGITNFILSENPRQIERIYTIFEDLKQSRKQPEDFEF
                     STLNISLHEGFVDRKLNLALYTDHQIFERYHKFHLKDSYSTREAITLKELYNLSPGDY
                     VTHIDHGVGRYDGLERVDVNGRQQEAIRIIYKNNDLLYISIHSLHRISKYIGKEGTEP
                     KIDRLGSGAWSKLKAKTKSRVKDIAKELIQLYAQRKKTEGFRFMPDTYLQTELEASFI
                     YEDTPDQVKATSDVKADMEKEYPMDRLICGDVGFGKTEIAVRAAFKAVADSKQVAVLV
                     PTTILALQHYKTFRDRLKDFPCRVDYLNRFKSQKKQKETLRELEEGKLDIIIGTHRLI
                     SQDVKFKDLGLLIIDEEQKFGVGAKEKLKQIKVNVDTLTLTATPIPRTLQFSMMGARD
                     LSIISTPPPNRYPIQTEVRPFSEEVIREAILYEVSRGGQVFFVHNRVLNIHDVERMLK
                     KFLPNITIGIAHGQMDGTHLENVMLDFIDGVYDVLLATTIIESGLDIPNVNTIIINDA
                     HHFGLSDLHQLRGRVGRSNRKAFCYLLAPPVSMLTDEARKRLRAIEEFSDLGSGFNIA
                     MRDLDIRGAGNILGAEQSGFISEIGFEMYHKILDEAMEELKESEFSELFRDEKPKPFV
                     KDCQIETDLEILIPADYITNSTERLIIYKELDGMENEEELASCKNRISDRFGPVPRQT
                     EELFNAIRLRWLAKEAGFEKIILKNERFIGYFISNQESPYYRSEKFTAMLRFVQENPS
                     ICRMKENESRLSLTFRNVRSIRDALALMYRIN"
     gene            4055..4513
                     /locus_tag="PKM34_04635"
     CDS             4055..4513
                     /locus_tag="PKM34_04635"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNQ82906.1"
                     /translation="MKRRVNWGMVLSHHPPCQYNRTFTLCRVRFCVRCTGILAGILLW
                     AIVFSVKDLPFYLTFITGFLFPLPAILNFTLNELGKRKNSNSKRTITGILMGLAIGMA
                     IGFFIANRFWPGIAIIAWIILLEFAVALVLRQAGVLDKFIREYEDAVYKD"
     gene            complement(4510..>5883)
                     /locus_tag="PKM34_04640"
     CDS             complement(4510..>5883)
                     /locus_tag="PKM34_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNQ82907.1"
                     /translation="TVADTTWYRWISRDPVATYLVVVSASNNWDLDIVYWDRPSTPGE
                     PMPIRFYYETGDNPVPMENIITDMATYFSDHYGEHPFEKDGFASVGQEFSWAGMENQT
                     LTSICPGCWYESLICHEFAHQWFGDMISPGTWADIWLNEAFATWSEAFWYENDGGYQA
                     YLNEIQGNASYYLATNPGWAVYEPEWAVETPDNTILFNYSITYMKSSCVLHLLRYALG
                     DSLFFPAIMDYATDTAEFKYKNAITDDFQAKMEESTGEDLDWFFDSWVKQPNHPVYEN
                     IYSIHDNNNGTWNVNFQVNQVQDNAGFFPLPVEISVFFAKYTDTTLRVMNDVNNQSFT
                     FTFDKEPGVVFFDLDNKIVLKQASLMVGTPEPEKAGKSLFLEQNFPNPVKNSTTLTYT
                     IPESGAVELVIYSTTGNSIKVLVNEVQEKGTHQVTADLSALTDGLYFCRLRASGKESV
                     IKLAVNH"
BASE COUNT         1472 a         1493 c         1497 g         1421 t
ORIGIN      
        1 tccttggagt catttgggaa aagctgccgt gtgatttcat catggtattc tttgatttgc
       61 ctgaacaacc ggcccgacct gccgtcggct gaaatcgtcg aagcgatcag ctcctccagt
      121 gcattggtcg ttttcccgaa agcggaaacc acaataacca gtgggatacc gggatatctc
      181 cggataatct ccggtatccg gcccactgcc ccggcggaat tcagtacgcc gccaccaaat
      241 ttgaaaactt tcatcgggat taattcgtga tcgctaccgg caaaaataat acttttgcag
      301 aactgtaagg gtaatttgaa cggttgaaaa cagaagaact tatccgcctt tacgctgccg
      361 cgccccaggt acaggccatg gccggcatcc tgaaaaacca gacctccccc aggattttcc
      421 tgcaggggct ggccgggtca tccccggccc tgatcaaagc agccgtcttt catcatctcc
      481 agcgcaacca tctcgtcatc ctcaatgatc ccgagtcggc agcctatctc tacaatgacc
      541 tggaaaccct tttcggagag aaagggcagc cctatgagca caaaaacacc cttttctttc
      601 ccacggtata taaacgccat ttcgaattcg acaaacccga ccggaccaat atgctgatgc
      661 ggtcggaggt gctgagccgg ctggtatcaa cccataaaaa aaccctgatc gtcagttatg
      721 ccgaagcctt atcggagaag gtggtccgca agaaagtgct gacaaagaac acgttgaaac
      781 tgagagcagg ggagaaggtc tcgcttgatt ttgtcgccga tatgctgatc gaatacggat
      841 tcgaccggga ggattttgtc tatgagccgg gccagttctc cattcgcggc ggcatcctcg
      901 acgttttttc gtttgcccac gataatcctt tccggatcga attcttcggg gaggaggtgg
      961 agtcgatccg taccttcgat ccggcatcac agctttccat agataaaatg gacaggatca
     1021 cgatcttgcc cgatctttcg gagcgccttg aggtggaatc ccgcgaatcg ttcctgtcgt
     1081 tcctgccgcc cggagccatg atctgggtgg atgacctggc cggattgcag gatttactgg
     1141 atgtatatac cgacaggctg caaaaactgc ttgacaacga cagggatggc ctgtatccca
     1201 agaacctgct cgggcatttt accggcaaag acgatttcct gaaagagttg ctcggtcatg
     1261 ccatcgtcga atcggggagc cggccggtac tgaaacccga cctcgccccc gaattcgata
     1321 tccggccaca acccaccttc aacaagaatt tcgacctgct caccgccgac ctggccgtca
     1381 atacaaaagc cggcatcacc aatttcatcc tgtcggaaaa tccccgccag atcgaacgga
     1441 tctatacgat ctttgaagat ttgaagcagt cgcggaaaca acccgaagat ttcgaatttt
     1501 cgacacttaa catctcgctg cacgaaggct ttgtcgacag gaagctgaac ctggctctct
     1561 acaccgacca ccagatattc gaacgctacc acaaattcca tctcaaagac agttacagca
     1621 cccgagaggc gattaccctg aaagagcttt acaatctcag ccccggtgat tatgtaaccc
     1681 atatcgatca tggcgtcgga cggtacgacg ggctggagcg cgtcgatgtc aacggaaggc
     1741 agcaggaagc catccgcatc atctacaaga acaacgacct gctttatatc agcatccact
     1801 cgctgcaccg gatctcaaaa tatatcggga aggaggggac ggagccgaag atcgaccggc
     1861 tgggatcggg ggcctggagt aaactgaaag ccaaaaccaa gagccgcgtc aaggatattg
     1921 ccaaggagct gatccagctt tatgcccagc ggaagaaaac ggaaggcttc cggttcatgc
     1981 ccgataccta cctgcagacc gagctggaag cttcattcat ttatgaagat acccctgacc
     2041 aggtcaaagc tacttctgac gttaaggcgg atatggagaa agaatacccg atggaccggc
     2101 tgatctgcgg ggatgtcgga ttcggcaaga cagagattgc ggtgagggcc gcctttaaag
     2161 ccgtagccga cagcaaacag gtggcggtcc tggtgccgac aaccatcctg gcccttcagc
     2221 attacaaaac tttccgcgac aggcttaaag attttccctg ccgcgtcgat tatcttaacc
     2281 ggttcaaaag ccagaagaag caaaaggaga cgttgcgcga actggaagag ggcaagcttg
     2341 acattatcat cgggacacac cggctgatca gccaggatgt gaaattcaaa gacctgggcc
     2401 tgctgatcat cgacgaggag cagaaattcg gcgtgggggc taaagagaaa ttaaagcaga
     2461 tcaaggtaaa tgtcgatacg ctgacgctga cggccacgcc gatcccaaga acgctccagt
     2521 tttccatgat gggcgcgcgc gacctgtcga tcatcagcac cccgccgccg aaccggtacc
     2581 cgattcaaac ggaggtcagg ccgttcagcg aagaggtcat ccgcgaagcg atcctgtacg
     2641 aggtgtcgcg cggcggacag gtgtttttcg tccataaccg ggtgttgaac atccacgatg
     2701 tggagcggat gctgaagaaa ttcctcccca acatcaccat cggcatagcc catggacaga
     2761 tggacgggac gcacctggag aacgtcatgc tcgattttat cgatggcgtg tacgatgttt
     2821 tgctggcaac tacgatcatc gaatcgggcc ttgatatccc gaacgtgaat accatcatta
     2881 tcaacgatgc gcatcatttc gggctcagcg acctgcacca gctgcgcggc agggtagggc
     2941 gctccaaccg gaaagccttc tgctacctgc tggcgccgcc cgtctccatg cttaccgacg
     3001 aagcccgcaa acggctccgc gccatcgaag aattctccga tctgggcagc ggcttcaaca
     3061 tcgccatgcg cgacctcgat atccgcgggg cagggaatat actgggggcc gaacagagcg
     3121 ggtttatctc ggaaatcggc tttgagatgt accacaagat cctcgacgaa gccatggaag
     3181 agctgaagga gtcggaattt agcgaactct tcagggatga aaagcccaaa cccttcgtaa
     3241 aggattgcca gatcgagacc gacctcgaaa tcctcattcc cgccgattac atcaccaact
     3301 ccaccgaaag gctgatcatt tacaaggagc tcgacggaat ggagaacgaa gaggaactgg
     3361 cttcctgcaa gaaccggatt tcggaccgtt tcggcccggt gccgcgccag accgaggagc
     3421 ttttcaatgc catccgcctt cgatggctcg ccaaagaagc cggttttgaa aaaatcatcc
     3481 tcaaaaatga acggtttatc ggatatttta tcagcaacca ggagtcgccc tactaccggt
     3541 cggagaaatt tacggccatg ctgcgctttg ttcaggagaa tccctctatc tgtcgtatga
     3601 aggagaacga aagccggctt tcgcttacct ttcgcaatgt caggtcaata cgtgatgctc
     3661 ttgcattgat gtacaggatt aattagcgcc ttcctggcag atttatggcc tttcatgatt
     3721 ttttttcaag ttttgttcca tctgttaaat atttatcaat aaatttacct cacctaaaaa
     3781 tggattaaga ttttttataa aacgggtaaa taataaccag ataaataact ccattattaa
     3841 ctaaaaacta aaagctatgg cctctccaaa gtggtgtaat ctgtgtaaca ggaatgttgt
     3901 tccgaagaaa aagtttaact ggctggtctt tatattcctg tgtggaatat tttatgtgcc
     3961 ttactatttg ctgaagaaag caaaatgccc catttgcgac ggagataact tcggtcctgc
     4021 aagggcaaat gatatgggca aatagcattt tttcttgaag agaagagtga actgggggat
     4081 ggtgctttcg catcatcccc cttgtcaata taaccggacc ttcaccctgt gccgggtccg
     4141 gttttgcgtc aggtgtacgg gtatcctggc gggaatttta ctatgggcga ttgtattttc
     4201 cgtaaaggac cttccttttt atctcacttt tattacagga tttctgtttc ccctgcctgc
     4261 catcctcaat tttaccctta atgaactggg caaacggaaa aattcgaaca gtaaacgaac
     4321 tattacgggg attttgatgg gattggctat aggtatggcc atcggattct ttattgcaaa
     4381 tagattttgg cccggaatag cgatcatcgc ctggatcatt ctcctggaat ttgcagttgc
     4441 attggtcctt cgccaggccg gtgtattgga taaatttatc cgggaatatg aagatgccgt
     4501 ttataaagat taatgattta cagccagttt gataacagac tcctttcccg atgctcggag
     4561 ccggcagaag taaagcccgt ctgtcaatgc tgaaaggtcg gctgtgacct ggtgagtccc
     4621 tttttcttgt acttcattca ccagcacttt tatgctattt ccggttgtcg aatagataac
     4681 aagttctact gccccacttt ccggaatagt ataggtcaat gttgtggaat ttttcaccgg
     4741 gttcgggaag ttttgttcca ggaaaaggct tttgccggcc ttttccggtt cgggagtgcc
     4801 caccatcagc gatgcctgct tgaggacaat cttgttgtcg aggtcgaaga aaactacgcc
     4861 cggctctttg tcaaacgtga aggtaaagga ctggttatta acgtcgttca tgacgcggag
     4921 cgtagtatcg gtgtatttgg caaagaaaac cgatatttcg actggcagcg ggaagaagcc
     4981 ggcattatcc tgcacctggt tcacctggaa gtttacattc catgttccgt tgttattgtc
     5041 gtggatggag taaatatttt cgtaaaccgg atggttgggt tgttttaccc aggagtcgaa
     5101 aaaccagtcg agatcttccc cggtcgactc ttccatcttt gcctggaagt cgtcggtgat
     5161 tgcgttttta tatttgaatt ccgccgtatc ggtggcataa tccatgatgg cggggaagaa
     5221 aaggctgtcg ccgagggcat accggagcag gtggagcacg caggagcttt tcatgtaggt
     5281 tatcgaatag ttgaacagga tagtgttatc gggggtttcc accgcccatt cgggttcgta
     5341 aacagcccat cccgggttcg tggccaggta atagctggca tttccctgga tttcattgag
     5401 atacgcctgg tatccaccgt cattttcata ccagaaagct tccgaccagg tggcgaaggc
     5461 ttcattcagc cagatgtcgg cccaggttcc gggactgatc atatcgccga accactggtg
     5521 ggcaaattca tggcagatca ggctttcgta ccagcagccc gggcagatgc tggtaagtgt
     5581 ctggttttcc atccccgccc agctaaattc ctggcctacc gaagcaaatc cgtctttttc
     5641 gaaaggatgt tcaccgtaat gatccgaaaa ataggtggcc atatcggtga tgatgttttc
     5701 catcgggacc gggttgtcgc cagtttcgta gtaaaagcgg atcggcatcg gttcgccggg
     5761 tgtggagggg cggtcccagt aaacgatatc caggtcccag ttgttgctgg ccgaaacgac
     5821 gaccaggtaa gtggctaccg gatcgcggct gatccaccgg taccaggtcg tgtcggctac
     5881 tgt
//
LOCUS       DAOQXS010000118         5873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13877_length_5873_cov_3.963389, whole genome shotgun sequence.
ACCESSION   DAOQXS010000118 DAOQXS010000000
VERSION     DAOQXS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13877_length_5873_cov_3.963389"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="ahcY"
                     /locus_tag="PKM34_04645"
     CDS             complement(<1..1188)
                     /gene="ahcY"
                     /locus_tag="PKM34_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNQ82908.1"
                     /translation="MENLVIDTNLKYKVKDISLAEWGRKEIGMAEKEMPGLMSLREKY
                     GPQKPLAGARISGSLHMTIQTAVLIETLTELGADVRWASCNIFSTQDHAAAAIAQRGI
                     PVFAWKGETLDEYWWCTYQALSFPGGKGPHLVVDDGGDATLLLHWGVQAEKEPSLLNR
                     KVESQDETAIMDLLRKVYVEDNRKWQRTTSEWRGVSEETTTGVHRLYQMQERGELLVP
                     AINVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVLGYGDVGKGCAKSMKS
                     YGARVVVTEIDPICALQAAMEGFEVNTLENVLSKGNIFVTATGNRDVITLEDMKRMND
                     EAIVCNIGHFDNEIQVGRLNEMPGITRVNIKPQVDKYIFPDGHSMYLLAEGRLVNLGC
                     ATGH"
     gene            1273..>1461
                     /locus_tag="PKM34_04650"
     CDS             1273..>1461
                     /locus_tag="PKM34_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82909.1"
                     /translation="MAEYLRRQIGNHSLLALWLITESTEELEAAISLRAEEKKLYESF
                     VAESRRKQWLAYRLLIRDL"
     assembly_gap    1462..1471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1472..1922
                     /locus_tag="PKM34_04655"
     CDS             <1472..1922
                     /locus_tag="PKM34_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNQ82910.1"
                     /translation="RPDDFPVEYDETNKPYLAGSNYHISVSHSGDMAAVIISRKSRVG
                     IDIEKIRPRVGKVKERFLSLPELDRIGNDSGFSRLTLAWSAKEAIYKLFGQRDLDFRE
                     NICLHLPDQPGEPFDGDIILDSGITRFRIENELIGDYILVYVEEPAP"
     gene            1900..2718
                     /locus_tag="PKM34_04660"
     CDS             1900..2718
                     /locus_tag="PKM34_04660"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNQ82911.1"
                     /translation="MSKSRRRDRFPAGSINFYTFASEQQEMTIYQKITGKIPGKAFAV
                     LIDPDQYHTGGIDNIVKIASDSRVDFILVGGSLLTNDHLDETLERIRSLAEIPVILFP
                     GSIMQVSGKADAVLLLSLISGRNADLLIGKHVIAAPYLKESKLEILPTGYMLIESGPL
                     TTAQYISNTLPIPHAKDDIAACTAMAGEMLGLRLIYMDAGSGAENPVSPSMIRKVKAV
                     ISVPLIVGGGIRTPEQALVSCHAGADIIVVGNAIEKDPSCLKEIAHAIHSCSEL"
     gene            2774..4906
                     /locus_tag="PKM34_04665"
     CDS             2774..4906
                     /locus_tag="PKM34_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNQ82912.1"
                     /translation="MKKNISLILVFLFATSLAFAQKKLTIEDVSGMNRSVYPSSMRNL
                     QWVPESNRFSFLENNALLTRKADKTVPDTLVRLSEMNPALKKASFDTLARFPFISWED
                     ESSFLFTNNQKVYRYFVNSRSVSFVNSYPEEAENIDATGNMKKIAYTVDNNLYLALEG
                     KQVQLTQDTLSGIVNGQSVHRNEFGIEKGTFWSPEGNYLAYYRKDETMVTDYPLVNTT
                     TRIAELENIKYPMAGMTSEQVTLVVFNLADGTRLTVKTGLPADQYLTSVTWSPDEQDL
                     YIGILNRGQNHLKLNRYSATTGEYEATLFEEKSDKYVEPLHPLYFIDSQPDRFIWMSQ
                     RDGYRHMYLYSTDGTLLRQLTSGPWIVTSFLGTDPKGNRAFFLATRNSPLNEDIFAVD
                     FKTASIQPVSANTGVHNPALSPDARYILDVYSDTSVCREYNIIDSKGKMLQVISRSDD
                     PLDDYVKCQSKIFQLPADDNTPLFCQMILPHDFNPAKKYPVIVYVYGGPHAQLVNNTW
                     LGGTSLFNYYLAQQGYIIFTLDNRGSANRGRDFEQAVFRNLGTLEVIDQMTGIRYLKS
                     LPYVDPERIGVTGWSYGGFMTISLMLRQPGVFKVGVCGGPVTDWKYYEVMYGERYMDT
                     PEENPDGYREASLLERAGELKGDLLIIHGTSDPTVVWQHSLALIQRFIEEGKLVDYFV
                     YPGHGHGVGGKDRLHLNRKIEKYFIDHL"
     gene            4903..>5873
                     /locus_tag="PKM34_04670"
     CDS             4903..>5873
                     /locus_tag="PKM34_04670"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNQ82913.1"
                     /translation="MIRWIIPPVILFFSLPGYFSATAQILLADSGLSDYRIVIPSKPS
                     AGESKAALQFQSVFREISSVTLPIVPDSRPDSEKEIVIGNSTRLEKIKSKSRFKDDED
                     DGFLIHTDQAKLFICGAGDNGLFYGVCSFFENYLGYRYYSPSVKHHPILNKVIINKID
                     DRQVPVFTFREMLFPARSDSAYRQWHKLDTHSDGSWGMWVHTFDDLVPAAKYFDEHPE
                     YFSEINGNRVRDGQLCLSNPQVFDLVIENLRKKIIARPEALYWSVSQNDNFLACQCAS
                     CDSVAKKFGGQSGLMIWFVNRVAELFPDKIISTLAYQYTRHAPENIRP"
BASE COUNT         1448 a         1596 c         1440 g         1379 t
ORIGIN      
        1 atggccggtg gcgcatccca ggttgaccag gcgcccttct gccagcaaat acatggagtg
       61 cccgtcagga aagatatatt tatccacctg cggtttaata ttcacgcggg ttatgcccgg
      121 catctcattc agtctgccaa cctgtatctc attatcaaag tgaccgatgt tgcagacgat
      181 cgcttcatcg ttcatccgtt tcatatcctc aagggtaatg acatcccggt tgccggtggc
      241 ggtgacgaag atatttccct tcgacagcac gttttcaagt gtattgacct caaatccttc
      301 catagccgcc tgcagggcac agatcggatc gatctcggtt acgaccaccc tggcgccata
      361 ggatttcatc gatttggcgc atccttttcc gacgtcgccg tagccgagca cgaccacaac
      421 tttgccggcc agcatgatat cggtagcccg tttgatgccg tcggccagtg attcgcggca
      481 tccatagagg ttatcgaatt tcgatttggt gaccgaatcg ttgacattga tagccgggac
      541 gagcaattca ccccgctcct gcatctggta gagccggtgc acccctgtcg ttgtttcttc
      601 ggaaacacct ctccattcgc tggtggttct ttgccacttc cggttatcct cgacatagac
      661 tttacgcaaa agatccataa tggccgtctc atcctggctt tcgactttcc tgttcagcaa
      721 cgaaggctct ttctcggcct gtacccccca atgaagcagc aaagtggcat caccgccatc
      781 gtcgacaacc agatgcgggc ctttgccgcc ggggaacgaa agggcctggt aggtgcacca
      841 ccaatattcg tcgagcgttt cgcctttcca ggcaaagacc ggtataccgc gctgtgcaat
      901 ggctgcagcc gcatgatcct gggtcgaaaa tatattgcag ctggcccatc ttacatctgc
      961 tcccaattca gtaagggtct cgatgagcac ggctgtctgg atcgtcatgt gaagagaacc
     1021 ggagatcctg gcccctgcca gaggtttctg cggtccgtat ttctcgcgga gcgacatcag
     1081 tcccggcatc tccttttcgg ccatcccgat ctcttttcta ccccattcag ccaggctgat
     1141 atcttttact ttatatttca aatttgtatc tattaccagg ttttccatat gtaaaaattt
     1201 gaggctgcaa aggtacgaaa attgtcaatg tggacgaatc ctagtacttt tggatttgat
     1261 aatcgtaacg gcatggctga atatcttcgc aggcaaattg gaaatcattc gcttctggcg
     1321 ttatggttga ttaccgaaag cacagaagaa cttgaagctg ccatttcact ccgggccgaa
     1381 gagaagaaac tctatgaatc ctttgtggcc gaaagccgca ggaagcaatg gctggcttac
     1441 cgtctcctca tccgggacct tnnnnnnnnn ncaggcccga cgattttccg gtggagtatg
     1501 acgagaccaa taagccttac cttgccggaa gcaattatca tatttcggtc agccattccg
     1561 gcgatatggc ggccgtcatc atcagccgca aatcaagggt cggaattgat atcgaaaaga
     1621 tcaggcccag ggtcggcaag gtgaaggaac ggtttctcag cctgcccgag cttgaccgga
     1681 ttggtaacga tagcggtttt agccggctga cgctggcctg gtcagccaaa gaagcgatct
     1741 ataagttgtt cgggcaaaga gacctggatt tcagggaaaa catctgcctg catctgcccg
     1801 atcagccggg agaaccgttt gatggcgata tcatcctgga ttccggtatt acccggttcc
     1861 ggatcgaaaa tgaactcatc ggggattata tcctggttta tgtcgaagag ccggcgccgt
     1921 gacagattcc cggcaggcag tataaatttt tatacttttg catcagagca gcaggaaatg
     1981 accatctacc agaaaatcac cggtaaaata cccggaaagg catttgcggt tttgatcgat
     2041 cccgatcagt accacaccgg tggcattgac aacattgtca agatcgcctc cgacagccgg
     2101 gtcgatttta tcctggtcgg gggcagcctg ctgaccaacg accacctgga tgaaacactg
     2161 gaaagaatca gatcactggc tgaaataccg gtcatccttt tcccgggcag catcatgcaa
     2221 gtcagcggca aagccgatgc ggtcctgctg ttatcgctga tttcagggcg taatgccgat
     2281 ctcctgatcg gtaaacacgt gatcgccgca ccttatttaa aggaaagcaa actggaaatc
     2341 cttccaaccg gatatatgct tattgaaagc ggcccgctga caacagcaca gtacatcagc
     2401 aacaccctgc ccataccgca tgccaaagac gacattgccg catgcacagc catggccggc
     2461 gaaatgctcg gcttacggct gatctacatg gatgccggtt caggcgccga aaatcccgta
     2521 tcgccctcca tgatccgcaa agtcaaggct gtaatctccg tcccgctgat cgtcggaggc
     2581 ggaatccgga caccggaaca agcccttgtc tcctgccatg ccggcgctga tattatcgtg
     2641 gttggcaatg ctattgaaaa agacccctcc tgcctcaaag aaatcgccca tgccatccat
     2701 tcctgttcag aactttaatc tcaccggcgg atttacccgt cggagcatca tatcgcaata
     2761 aataataccg aatatgaaaa aaaacatctc cctgattctc gttttccttt tcgccacatc
     2821 cctggcattt gcgcaaaaaa aactgaccat cgaagacgtc agtggcatga accgctcggt
     2881 ttatccgtca tcgatgagaa acctgcaatg ggtgcccgaa agcaaccgct tttcattcct
     2941 ggagaacaat gccctcctca cccgtaaagc cgacaaaacg gttcccgata cactggtcag
     3001 gttatccgaa atgaacccgg cactgaaaaa agcatcattc gacaccctcg caaggtttcc
     3061 tttcatcagc tgggaagacg aatcttcctt cctgtttacg aacaaccaga aagtgtaccg
     3121 gtattttgtc aacagccggt ctgtttcatt tgtcaattcc tacccggaag aagctgaaaa
     3181 tattgatgca actggcaata tgaaaaagat tgcctatacc gtcgataata acctctattt
     3241 agctttggag ggaaaacagg ttcaactgac acaggatacc ctttcgggta ttgttaacgg
     3301 gcagagtgtt caccgcaatg agttcgggat agaaaaaggg accttctggt cgccggaagg
     3361 caattacctg gcttactaca ggaaggatga aacgatggtg accgattacc cgctggtcaa
     3421 caccaccaca cggattgcag agctggaaaa catcaaatac ccgatggccg ggatgaccag
     3481 cgaacaagtg acgcttgttg ttttcaatct ggctgatggc acccggctga cggtgaaaac
     3541 cggtttgcct gccgatcagt accttacctc ggtgacctgg agccccgatg aacaagacct
     3601 ctacatcggg attctcaaca ggggacagaa tcacctgaaa ttgaaccgtt atagcgccac
     3661 taccggggag tacgaggcca ccctcttcga agaaaaaagc gataaatatg tcgagccgct
     3721 gcatccgctc tatttcattg attcgcagcc cgaccggttc atctggatga gccagcgcga
     3781 tggataccgc catatgtacc tttattcgac cgacggaacc ctgctccgcc agctgaccag
     3841 cggcccatgg atcgtcacct ccttcctggg aaccgacccg aaagggaaca gggcgttttt
     3901 cctggccacc cggaacagcc cgcttaacga agatattttc gccgttgatt tcaagacggc
     3961 ttctatccag cccgtctcgg ccaacaccgg tgttcacaac ccggcgctca gccccgatgc
     4021 caggtacatc ctggatgttt acagcgatac cagcgtctgc cgggaataca acatcatcga
     4081 ctcgaaaggg aaaatgctcc aggtcatttc gcgcagcgac gacccactgg atgactatgt
     4141 aaaatgccag agcaagatat tccagctccc ggccgacgac aacaccccgt tgttctgcca
     4201 gatgatcctg ccgcatgatt tcaacccggc aaaaaagtac ccggtcattg tgtatgttta
     4261 cgggggtccc catgcacagc ttgtcaacaa cacctggctc gggggaacca gcctgttcaa
     4321 ttactacctg gcgcagcagg gctatatcat tttcacgctc gataacaggg gttcggccaa
     4381 ccgcggcagg gacttcgaac aggctgtttt ccgcaacctg gggacgcttg aagttattga
     4441 ccagatgact ggtatcagat acctgaaaag cctgccatat gtcgacccgg agaggatcgg
     4501 cgtaaccgga tggagctacg gcggattcat gacgatttcg ctgatgcttc gccagcccgg
     4561 tgtattcaaa gtgggcgttt gcggagggcc ggtgaccgac tggaaatatt acgaagtgat
     4621 gtacggcgaa cggtacatgg ataccccgga ggaaaatccg gacggctatc gtgaagccag
     4681 cctgctggag cgcgccggag aactgaaggg agatctgctg atcatccacg gcacctccga
     4741 tccgacagtg gtctggcagc attcgcttgc cctgatccag cgctttatcg aggaagggaa
     4801 actggtcgat tattttgttt acccgggaca tggccatggc gtgggcggaa aagaccggct
     4861 gcacctgaac cggaagatcg aaaaatattt tatcgatcac ctatgatccg gtggataatc
     4921 ccgccggtca tcctgttttt ctcattacca ggatatttct ccgccaccgc ccagatcctg
     4981 ctggccgaca gcggcctctc ggattaccgg atcgtgatcc ccagcaaacc ttctgccgga
     5041 gagtcgaaag ccgccttgca gtttcaatcg gttttcaggg aaatatcatc agtgacgctg
     5101 ccgatagtcc ccgactcccg gccagactcg gaaaaggaaa tagtcatcgg gaacagcaca
     5161 cgcctggaaa agattaagtc caaaagccgt tttaaagatg atgaagatga tggattcctg
     5221 atccatactg accaggctaa gcttttcatc tgcggagcag gcgacaatgg attattttac
     5281 ggggtttgct cattctttga gaattacctt ggttaccggt actacagtcc ttcggtaaaa
     5341 catcatccta tacttaacaa ggtcataatt aataagatag acgacaggca agtccccgtc
     5401 ttcactttca gggagatgct tttccctgcc cggagcgatt cggcttacag gcaatggcat
     5461 aagctggata cccactccga cggctcctgg ggtatgtggg tccatacttt cgatgacctg
     5521 gtcccggctg ctaaatattt tgacgaacac ccggagtact tttcggaaat aaacgggaac
     5581 agggtccgcg acgggcagtt gtgcctttct aatccccaag tatttgacct ggtgattgaa
     5641 aatctccgaa aaaagatcat cgcacggccg gaagcccttt actggtccgt ttctcaaaac
     5701 gacaacttcc tggcctgcca gtgcgcttcg tgtgattcgg ttgcaaaaaa attcggcggc
     5761 cagtcaggat tgatgatctg gttcgtgaac cgggtggcgg aactcttccc ggataagatc
     5821 atctccacac ttgcctacca gtacactcgt catgctccgg aaaatatccg gcc
//
LOCUS       DAOQXS010000119         5857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_13920_length_5857_cov_3.675801, whole genome shotgun sequence.
ACCESSION   DAOQXS010000119 DAOQXS010000000
VERSION     DAOQXS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_5857_cov_3.675801"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PKM34_04675"
     CDS             complement(<1..1821)
                     /locus_tag="PKM34_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HNQ82914.1"
                     /translation="MDMQSFDAVIFDLDGVITKTALVHSAAWKKMFDGYLREREIKYG
                     EPFREFSQKEDYLPYVDGKPRYKGVESFLESRGIKLPFGTPEDDPEKETVCGIGNRKN
                     IAFNEALKSDGVQVYESTVALIRRLKEEGIRVGVASSSKNCRQVLETAGLLDMFETRV
                     DGEVSAELGLKGKPEPDIFTVAADNLGVTYDRAVVVEDAVSGVQAGRKGNFGLVLGLA
                     REENENELYSNGADIVVKDIADIGLEGIVDWFRQGLFKDNWSLTYHDYIPEREKTREA
                     LLTVGNGYFGTRGAMPETIAGPYNYPGTYMAGIYNRLVSKVGDKEIENEDFVNVINWL
                     PVTFKINDEEWFDVNNARIAEVRRTLHFNNGMLYRRLVVRDDAGRETLIGTRRIASMD
                     NPNLAAQEYTVTPLNYSAKITIRSGLYGDHINAGVERYSQLNQKHIEPVLLGSEENIQ
                     YMVVKTTRSQNVIAAAAQVTVFEGNDRVYVHFHKNHTAGRSRIEFTKKLAQGEALRIQ
                     KIVALCHNRDQDETDPLDSAIENCKKFRTFDEVLEKSARKWEEIWDRIDIRVAGDRLA
                     QKLLRLHLYHTLITTSPLNRNIDAGIPARGLHGEAYRGHIF"
     gene            complement(1850..3562)
                     /locus_tag="PKM34_04680"
     CDS             complement(1850..3562)
                     /locus_tag="PKM34_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ82915.1"
                     /translation="MKDRFSDLYRIWNILYPEHPTDKLDALIGEILSFSGKTAIPPLE
                     EEWYKNAVVYSLYVDLFSSDFKGLTGRLEYLQDLGVNCLWLLPILDSPMRDAGFDISH
                     YRKIRKDLSGLGEKATDDDLDRIFRDFLDEAHRRGMKVIFDIATNHVSDQHPWFREAI
                     KSKDNPYRDYFIWSPTGEEYAGARIIFYGIEESNWKKEGDEYYFHRFFDFQPDLNYRN
                     PEVLAEMSRNLLFWLERGVDGFRADAIPYIWKEEGTPCENHPNTHNIVKFFRAVIDHA
                     RPNTLLLAEACQKPWEVVKYFGDGDECNAGYHFPLMPQIFKAVAMGSSQPVMHTLSTA
                     VTPEIPGSAQWFTFLRCHDELSLELVYVTEEDRKYIHDHYCRKPQWDFRRGQGISARL
                     SELMERNPKKIGMIFSIMLTLPGTPVIYYGDEFGKFNDEEYYNEMKISVGKDDTRFLV
                     RGKIDWKRVENALADDSTFESAVFSQIRQMLHARNSLAVFGRGSMEWVHLRSGDGNPA
                     ASVMAYIRSLDGKRVLVIHNLSGNTVPVKWEFPGGKTILGPDPVRNGDLMEISGFGYH
                     WLTL"
     gene            3921..5771
                     /locus_tag="PKM34_04685"
     CDS             3921..5771
                     /locus_tag="PKM34_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNQ82916.1"
                     /translation="MKATLIRILSAIILAIPLVGFGQAEIKRIDPPFWWTGMAENRLQ
                     LMVYGEGLGQVQPALSYPGVKMTGYSALENPYYLVIDLEISENARPGKVPISFQYQRE
                     LLFVLDYELRARTSGSAERKGFGKEDVIYLIMPDRFSNGDPANDTISGMFEPASHANP
                     DGRHGGDLKGITDLLPYLADLGVTALWLNPVLENNQPAYSYHGYAITDFYRVDPRFGS
                     NEGYLEFVGKAHQEGLKVIMDMVFNHCGSNHLWIQDLPSHDWIHQFPEYTRSNFRAET
                     VMDPYASEFDRTKMMTGWFDKPMPDLNQHNEYLANYLIQNSIWWIEYAGLDGIRMDTY
                     PYSYKDFMTRWMERINKEYPGFSVVGETWLQKEAHTAYWQSGSAAGAGAVTHLDHVTD
                     FPLQYAIKDAFTGEEGWTSGLARIYYVLSQDYLYADPFGNVIFVDNHDLTRFYSSIGE
                     NFAKFRMAMAILLTTRGIPMIYYGTEYLMTGEEPKGHGYIRENFPGGKWQSAVGSWQS
                     VAGGEELGVEFLKGLLSWRKTSEVVHSGKLMQFVPDDGIYVYFRYNATESVMVIINKN
                     TTDKALKLNRFQERLEKYSRGKDIITGQEYLLGEDLSIPPETVLILELSH"
BASE COUNT         1411 a         1453 c         1459 g         1534 t
ORIGIN      
        1 gaagatatgt ccgcggtagg cttctccatg gagcccgcgc gcgggaatcc ctgcatcgat
       61 attccggtta aggggtgaag tggtgatcag ggtatggtac agatggagtc tcaatagttt
      121 ttgggcgaga cggtcgccgg cgacacggat atcgatccgg tcccagatct cttcccattt
      181 ccgggccgat ttctcgagga cttcatcgaa ggtcctgaat tttttacaat tttcgatggc
      241 cgaatcgagg gggtcggttt cgtcctggtc cctgttgtgg caaagggcta cgatcttctg
      301 tatccgcaga gcttctccct gggccagttt tttcgtgaat tcgatccggg agcggccggc
      361 ggtatggttt ttatgaaaat gtacatagac ccggtcgttt ccttcaaaaa ccgtcacctg
      421 tgccgcggct gcgatgacat tttgcgaacg ggtggtttta accaccatat actggatatt
      481 ctcttccgag cccagcaaca ccggttcgat atgtttctgg ttcaactggc tgtagcgctc
      541 cacgccggca ttgatatgat cgccgtacaa accggaccgt atagtgattt tagcggaata
      601 gttcaggggt gtgaccgtat attcctgcgc ggcaaggttc ggattgtcca tgctggctat
      661 ccgtcgggtc ccgatcagcg tttcgcggcc tgcatcatct ctcacgacca gcctgcggta
      721 aagcatgccg ttgttgaaat ggagcgtgcg gcgcacttca gcgatcctgg cattatttac
      781 atcgaaccac tcctcatcgt tgattttgaa agtaacaggg agccagttga tgacattaac
      841 aaagtcttca ttttcaattt ccttatcgcc gactttggag accagccggt tatagatacc
      901 ggccatatag gttccgggat agttatatgg accggcgatg gtttcgggca tggcgcctcg
      961 ggtcccgaaa tacccgtttc caactgtaag cagcgcttca cgggtttttt ccctttcggg
     1021 gatgtaatcg tggtaagtta gcgaccagtt gtctttgaaa agcccctggc ggaaccagtc
     1081 gacaatccct tcgaggccga tatcagcgat atctttgacc acgatatcgg ctccattaga
     1141 ataaagttcg ttttcatttt cttcccgggc caggccaagg acgagaccga aattgccttt
     1201 acggcctgcc tggacaccgc tgacggcatc ttcgacgacc acagcacggt cgtaagtcac
     1261 gccgagatta tcggctgcca cggtgaagat atcgggctcc ggtttgcctt tcagtcccag
     1321 ttcggccgac acttccccgt ctaccctggt ttcgaacata tcgagtaatc ctgcagtttc
     1381 caggacctgg cggcagttct tgctgctgga agccacaccc acgcggatgc cttcttcttt
     1441 cagccggcgg atcagggcta ctgtcgattc gtaaacctgt acgccatccg atttcaacgc
     1501 ttcattgaag gcgatattct tccggttccc tatcccgcaa acggtctcct tttccggatc
     1561 atcttccggg gttccgaaag ggagctttat ccctctcgat tcgaggaaag actcgactcc
     1621 tttgtacctc ggcttgccgt cgacataagg caggtaatct tctttctgag aaaattccct
     1681 gaagggttcg ccatatttta tttccctctc cctgagatac ccgtcgaaca ttttcttcca
     1741 ggcggcacta tgcaccagcg ctgttttggt gatcacccca tcgaggtcaa aaataaccgc
     1801 atcaaaagat tgcatatcca tttctaaata ttcctcttct taaaataatt tacaaagtca
     1861 accaatgata tccgaaaccg cttatttcca tcaagtctcc gttccttacc ggatcaggcc
     1921 cgaggattgt ctttccaccg ggaaactccc atttaaccgg aacagtattc cctgaaaggt
     1981 tatggatgac cagtaccctt ttaccatcca gggaacggat gtaagccatc acacttgcgg
     2041 cagggttacc gtcacctgac cgcagatgaa cccactccat cgatcctctt ccgaaaaccg
     2101 ccagtgaatt ccgggcatgg agcatctgcc ggatttgtga gaaaactgcg gattcaaagg
     2161 tgctgtcgtc agccagtgca ttttccaccc ttttccagtc gatcttaccc ctgaccagga
     2221 accgtgtgtc gtctttcccg acacttattt tcatctcgtt gtagtattct tcgtcattga
     2281 actttccgaa ctcatcgcca tagtagatca cgggtgttcc gggcaatgtc agcataatgg
     2341 aaaagatcat cccgattttt ttgggatttc gttccatcaa ttccgaaagc cgggctgata
     2401 tgccctgtcc gcgacgaaaa tcccattgtg gttttcggca atagtgatca tggatatatt
     2461 tccggtcttc ttcggtcaca tagaccagtt ccaggctcag ttcatcgtgg cagcgcagga
     2521 aagtaaacca ttgggcgctt ccgggaattt cgggtgtgac ggcggtactc agcgtgtgca
     2581 tgaccggctg gctgctgccc atagccactg ccttaaagat ctgcggcata agcgggaaat
     2641 gataaccggc attgcattca tccccatcgc cgaagtattt cacgacctcc cagggtttct
     2701 ggcatgcttc ggctaaaaga agcgtattgg ggcgggcatg atcgatcacg gcccggaaga
     2761 atttcacaat gttgtgcgta ttaggatggt tctcgcaggg ggttccctct tctttccaga
     2821 tgtacgggat cgcatcggcc cggaaaccgt cgacgccacg ttccagccag aacagcaggt
     2881 ttcttgacat ctccgccagc acctccggat tacggtagtt caggtcgggc tggaagtcga
     2941 aaaaccggtg aaaataatat tcatctccct cttttttcca gttactctcc tcgataccgt
     3001 aaaaaatgat acgggcgccg gcatattctt caccggtcgg gctccagata aagtaatcac
     3061 ggtaaggatt atctttggat ttaatcgctt ccctgaacca ggggtgctgg tcggagacat
     3121 ggtttgtcgc gatgtcgaag ataactttca ttcctcttcg gtgcgcctca tccaggaaat
     3181 ccctgaaaat ccggtcaagg tcgtcatcgg tcgctttttc acccaggccc gacagatctt
     3241 tcctgatttt ccggtagtgg ctgatatcaa atcctgcgtc gcgcatgggc gaatcgagaa
     3301 ttggcagtag ccacaggcag ttaaccccca aatcctgcag gtattccagc cgacctgtca
     3361 aacccttgaa atcgctgctg aacagatcga cgtaaagcga atatacgaca gcgtttttat
     3421 accattcttc ttccagtgga gggatggctg tttttcctga aaaggacaag atctcgccaa
     3481 tcaaggcatc cagtttgtcc gtcggatgtt ccgggtataa tatattccag atccggtaaa
     3541 gatcagaaaa tctatctttc atgcacaatc ggtaaaattg ctgataacca aataaatcca
     3601 gaaaaatata aaccggatta attgtccggc aaaagtataa atttatccat tgtgatttta
     3661 aaatgcaagc ctcaaaaaca aaaaagcccc ggactaacgt tcagggcttt tttcagacag
     3721 ggaagtttct cggctattat tggctgactc taacagcaac ccgtcttgga acgatccggc
     3781 cggttcaaat tccgaatgta agtttactat aataatccgg gatattttca ggatttaacg
     3841 attacttatg aataggtcac tgttgaaaaa tgatccggtt aattttccgt aacttcgcgg
     3901 ccgctaagat aaaaatcctt atgaaggcaa cccttatcag aatattatcg gcaattatat
     3961 tggcaattcc tttggtggga tttggccagg cggagataaa aaggatcgat ccgccgttct
     4021 ggtggaccgg catggcagag aacaggcttc agcttatggt ttatggcgaa gggttgggcc
     4081 aggtgcaacc ggctctcagc tatccgggtg tgaaaatgac aggttattcc gcgcttgaga
     4141 acccctatta cctggttatc gatctggaga tctcggaaaa tgcacgaccc ggtaaagtgc
     4201 caatcagttt tcagtaccag cgtgagctgt tgtttgtgct ggattatgaa ctgagggccc
     4261 ggacgtcagg atcggctgag cgcaagggtt ttggaaagga agacgtgatc tacctgatca
     4321 tgcccgatcg cttttccaat ggcgacccgg ccaacgacac gataagcggg atgttcgaac
     4381 cggccagtca tgccaatccc gacgggcggc acgggggcga tctcaaaggg attaccgatc
     4441 tgcttcccta cctggccgac ctgggtgtaa ctgccctctg gctgaacccc gtgcttgaaa
     4501 ataaccagcc tgcctattct taccacggat atgccattac cgatttttac cgggtcgacc
     4561 caaggttcgg gtcgaatgaa ggttatcttg aatttgtcgg aaaagctcat caggaggggc
     4621 tgaaagtcat catggacatg gtgttcaatc attgcggaag caaccatctc tggattcagg
     4681 atttgccttc gcatgactgg atccatcaat ttccggaata cacccgatcc aatttcaggg
     4741 cggagaccgt tatggacccg tatgcttccg aattcgaccg gacgaaaatg atgacaggtt
     4801 ggttcgacaa gccgatgcct gacctcaacc agcacaatga gtatctggcc aattatctga
     4861 tacagaacag catatggtgg attgaatatg ccggtcttga tgggatcagg atggatactt
     4921 atccctattc ttacaaagat ttcatgacac gctggatgga gaggattaac aaggaatatc
     4981 ccggattcag cgtagttggc gagacctggc ttcagaaaga ggcccatacg gcttactggc
     5041 aatcgggttc agctgctggc gccggagccg ttacccacct ggatcatgtc accgattttc
     5101 cgctgcagta tgctataaaa gacgcattca ccggggagga agggtggacc agcgggcttg
     5161 cccggattta ctatgtgctt tcgcaggatt acctttacgc ggatccgttc ggtaatgtta
     5221 ttttcgttga taaccatgac cttacgcggt tttattcgag catcggcgaa aatttcgcca
     5281 aattcaggat ggctatggct attttactga caacgcgtgg catcccgatg atttattacg
     5341 gaacggaata cctgatgacg ggtgaagagc cgaagggtca cgggtatatc cgggagaatt
     5401 tcccgggagg gaagtggcag tcggcggtcg gcagttggca gtcggtagcc ggaggtgagg
     5461 aattaggggt tgagttttta aaaggattgc tgagttggag gaagacctcg gaggtggtgc
     5521 acagcggtaa gctgatgcag tttgtgccgg acgacggcat ctatgtatat ttccgttata
     5581 atgcaacaga atccgttatg gtcatcatca ataaaaacac aacggataaa gcactgaaat
     5641 taaacaggtt tcaggagagg ctggagaaat attcccgggg taaggatatc attaccggtc
     5701 aggaatatct attgggcgaa gatctttcca ttccgccgga aacggtttta atcctggaac
     5761 tatcccatta aatgaaaaat gaaaaacgta caatgcaaaa cggaacatgt caatgtccga
     5821 tgctcagtgc gtgcattccc catccctgca aaatgat
//
LOCUS       DAOQXS010000120         5833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14010_length_5833_cov_3.832987, whole genome shotgun sequence.
ACCESSION   DAOQXS010000120 DAOQXS010000000
VERSION     DAOQXS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_5833_cov_3.832987"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKM34_04690"
     CDS             <1..521
                     /locus_tag="PKM34_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82917.1"
                     /translation="IVMVLLVLGFTGCDKVSDLNTVTFDADFAASLDCSTAALKSTAE
                     NHAFSGTAVIDPASDTNVSDYLDNIKKYEVEAIHATITSISQENVTVYTATLDVFNAG
                     RETGWTMTNELLTTGKTITLGNENGQWDTVSEILKDGQVFTVQINGEADTGGVTFTLR
                     VRIETKVEAKVI"
     gene            746..1927
                     /locus_tag="PKM34_04695"
     CDS             746..1927
                     /locus_tag="PKM34_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ82918.1"
                     /translation="MLDSETYSPLINANISLTGSRTGCTSGLDGAFTIQVDTLPVYMI
                     ISYLGYETQHIWIDSKAEGLNVLMKPTASLLKEIEISSGKPEVFFKDDKYSILDYTID
                     HSFIYMIIFRYRTARAELICKKDNGEIIARSCTLPFKPTRLFADCLGYIHLLSPDSSY
                     QVFLNKDTLLFPHRATLSKFYATLSDCITSTADWLYFRKESNDKLSVSFYRINRHSGM
                     RSEINTYRDEEAFRMLRDHPYDYYLLTMDTLPASFNEMITYNWVKKIQYKSNLSVMEK
                     TGDSLVIINTTDGSVDILNISGYLINHLQMPLSKHTGEKWMKNILNDPVSHCLYTTFL
                     KNGRLHLYKLDLTKGHLQKTLTLAHSFPQHVMVHNNYLYYLYDVPGLMDNKQLLRESI
                     R"
     gene            2045..2452
                     /locus_tag="PKM34_04700"
     CDS             2045..2452
                     /locus_tag="PKM34_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNQ82919.1"
                     /translation="MMKQLKSTGRELTKAEEQIMHIFWDKGSALVHDILKEFPEPKPA
                     YNTVSTIIRILEQKGFIGHKAYGRTYEYYPLISKNDYTRTYFRSFVANYFGNSYKSLA
                     SFFASEEKISIEELEEIRRLMDMEIQKQKAQKK"
     gene            2449..3735
                     /locus_tag="PKM34_04705"
     CDS             2449..3735
                     /locus_tag="PKM34_04705"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNQ82920.1"
                     /translation="MTDLLTYLLHSSAILAALVTVYWLFLRKETFFHVNRFYLVFSIL
                     LSLTMPFIRIGFFETETFRPAAILLEPVLITGEKVDKIAGSNISAFQLVAIVYFTGVV
                     IFTAKFIFQLIQLAVITRRFKIIRRDGSRFVLVDNGYSPFSFFNFIYIREEYLSDDKF
                     APVVAHEKVHIRQIHTIDIILAEFAVILQWFNPFAWLAGHAIKRTHEFLADEGVLKDG
                     FQKQKYQEFILQESIGIQLNTLTNNFNVSLLKNRIIMMTKKRSASWAKLKAGFALPSL
                     AVVLFLFTAGNPLWLTAQESQQKSEQAKAAQTVSQSAQEEPAFQVVKNMPSFKGGMDA
                     MTKYLVENIKYPPEAKTKGTAGTVFVTYVIEKDGSVTNVKILQGIGDGCDEEAARVVK
                     GMPKWNPGTNDNNEPVRVIFNLPIKFSLEGKKEEVK"
     gene            complement(3736..>5833)
                     /locus_tag="PKM34_04710"
     CDS             complement(3736..>5833)
                     /locus_tag="PKM34_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82921.1"
                     /translation="SDTVFIYSRLEIEASGDFQLCQNGESDIEIVSTNVGDDYSTIWY
                     FENPPGVLTQVGNESVYTVTWEDYGSFAPNTLTFICTATDECGNSANDTVHATFFPVV
                     TISGVPLICLYDEIQLTCSFALQYWWHYGSLAGPLLGQNQVLNYTPPNSGLHTIWVRI
                     ENECHEYADTSFTFEVSELVMDMTMNNSTNFFTCPNVPFTLQELHAFDGWYWTWEDDG
                     TNYEAYGQNISLEMTEAGPHPVTVIAYNNHGCYDTITRTVTVYPYAYPVASTSIESVC
                     IDYPTDLSIVPTGPVTISSYFWTANPAYAWQPGQQTQASPTVTPQETTTFKCRITDNH
                     GCLDSATVVVNVRPRLAGNILGDPGFQCTDKPVTITFLPTVTPLPDATYSWTFDDGVP
                     ATSNLAVPPQIVWSTDGLKNISLHIEELGCEETFYFQYQVYPDPLAGFTATGAAGCQP
                     IQVSFTNISSNLENPSYLWDFGDGTTGTDVNPSHEYTIPGTYDITLTVTNSTGCVNTL
                     TINDMVEVFEVPVADFTADPQAATIDNPTINFSEQVNIPYQIINWDFGDGNEDLSSPN
                     PRHTYTLPGTYLVVMYTETEHGCWDRDTLEIGIVEDIKIFVPNAFTPNGDGLNDCFAI
                     GGTTGDIVDVFRIIIYNRWGVQLVDEPVHNPECLWNGRDVNGVEMPGGQYIFRIFGKN
                     LRGSKKVYEGMVTLLR"
BASE COUNT         1658 a         1353 c         1302 g         1520 t
ORIGIN      
        1 tgatcgtcat ggtgttactt gtgctgggtt tcaccggatg tgataaagtc agtgatctca
       61 acacggtcac ttttgatgcc gatttcgccg catcattaga ttgctcaacg gctgcattaa
      121 aatccactgc cgaaaatcat gcgttcagcg gaactgcggt aatcgatccg gcatcggata
      181 ccaatgtcag tgattatctg gacaatatta agaaatatga ggtagaagct atccatgcaa
      241 ccattaccag tatatcgcag gagaatgtga ccgtgtatac agctacgctg gatgttttca
      301 acgccgggcg tgaaaccgga tggacgatga caaacgaatt gctgacaacg gggaaaacca
      361 tcacgctggg caatgaaaac ggccagtggg atacggttag tgagatcctg aaagatggcc
      421 aggttttcac cgttcagata aacggagaag cagatacagg gggtgtaaca tttactttgc
      481 gggtgagaat tgaaacaaag gtggaagcca aagtgatata gttctctgtg agactccatc
      541 tcgaaaaatc gagatggagt ctcacttttc catccgttaa cccaaacgct caaagggtta
      601 aaacagtgaa gtatacggtg tttaaattgt ttagatttaa acacaaaact ccaatgtaat
      661 gacccgatgc aggtttttgt ccgtcattct actctcatta attttctttt ctctccatgc
      721 tcagaagcat cccatctcag gtgttgtgct tgattctgag acctattcgc cgcttattaa
      781 tgcaaatatc agcctgacgg gcagccggac aggttgcaca tcaggcctcg acggggcatt
      841 caccatacaa gtcgatacct tgccggtcta tatgattatc agctacctgg gatatgaaac
      901 acagcatatc tggatcgaca gcaaagcgga aggtttgaat gtgctcatga aacccacagc
      961 cagtcttttg aaagagattg agatatcttc cggaaaaccc gaagtattct ttaaagatga
     1021 taaatactct atcctcgatt acactatcga ccactcattc atctatatga tcatcttcag
     1081 gtaccggaca gccagggcag aactgatctg taaaaaggac aacggggaga tcattgcgcg
     1141 gtcctgtacc ttgccattca aaccgacacg attatttgcc gattgccttg ggtatataca
     1201 cctgcttagc cccgattcat cctaccaggt attcctgaat aaagacacac tgcttttccc
     1261 tcaccgggcc actctgtcga aattctatgc caccctttcc gattgtatca cgtcaaccgc
     1321 agattggctc tatttccgta aagagagcaa cgacaaactt tcggttagct tttacaggat
     1381 caaccggcat tcggggatgc gatccgaaat caacacctat cgggatgaag aggcattcag
     1441 gatgcttcgc gatcatccgt acgattatta ccttttaact atggatactt tgccagccag
     1501 tttcaatgag atgatcacat ataactgggt aaaaaagatc cagtacaaat ctaatctgtc
     1561 ggtgatggaa aaaacaggcg attcgctggt tatcattaat acaacggatg gctctgtcga
     1621 tattctgaat atcagcggat acctgatcaa tcatttgcaa atgccccttt cgaagcatac
     1681 gggtgagaaa tggatgaaaa acattttgaa tgatcctgtc tcgcattgct tatatactac
     1741 ttttcttaaa aacgggaggt tgcatcttta caaactggac ctcaccaaag gccatcttca
     1801 aaaaacctta acgctggccc acagcttccc gcaacatgtc atggtccaca ataactacct
     1861 gtattacctg tatgatgtgc cggggttgat ggataacaag caattgctca gggaaagtat
     1921 tcggtaagat taacgtgaga ctccatctcg atttctcgag atggagtctc ataaaaaaaa
     1981 taattacttt tttagttgac aaactatttt tttagttata tatttgcagc ataaaaatta
     2041 actaatgatg aaacaactga aaagcacagg gcgcgaactc accaaagccg aggaacaaat
     2101 tatgcatata ttctgggata aaggatcggc gctggttcat gatatcctga aagaatttcc
     2161 ggaacccaaa ccggcataca ataccgtttc cacgatcatc cggatcctgg aacaaaaagg
     2221 gtttataggc cacaaagcgt atggccgaac ttatgaatat taccccctga tctcaaaaaa
     2281 tgattacacc cggacctatt tccgcagttt cgtagccaac tatttcggta actcctataa
     2341 atcactggct agtttcttcg cgtctgaaga aaagatcagc atcgaggaac tggaagaaat
     2401 ccgaaggctg atggacatgg agatccaaaa acaaaaagca caaaaaaaat gacagacctg
     2461 cttacatacc tgttgcattc atcggctatc cttgccgcac tggtgaccgt ttactggctc
     2521 tttctcagga aagagacctt cttccatgtc aaccggttct acctggtctt ttctatactt
     2581 ctttcactga ccatgccatt catacggatt ggtttttttg aaacggagac cttcaggcct
     2641 gccgccatcc tgcttgaacc ggtcctgatt acgggagaaa aagtcgacaa aattgccggc
     2701 agcaatattt cagcctttca gcttgttgca attgtttact tcacaggagt agtaatattc
     2761 actgcgaagt tcatttttca gcttatccag ctggctgtca tcaccaggcg atttaaaatt
     2821 atccggcgcg atggatcccg tttcgtcctg gtggataatg gctactcccc cttttcattc
     2881 ttcaacttta tttatatccg ggaagagtac ttatctgatg acaaattcgc tccggttgtg
     2941 gcgcatgaaa aagtacatat ccggcaaata catacaatcg acatcattct ggcggagttt
     3001 gcggtcattt tacaatggtt taatccattt gcctggctgg ccggccatgc tatcaaaaga
     3061 acccatgaat ttctggcgga tgaaggcgtt ctgaaggatg gttttcagaa acaaaaatac
     3121 caggaattta ttctgcagga atctattggc attcaattaa ataccttaac aaataacttt
     3181 aatgtttcat tacttaaaaa cagaattatt atgatgacga aaaaacgctc cgcatcatgg
     3241 gctaaactaa aggccggatt tgcactgccc tcactggctg tggttctttt tcttttcaca
     3301 gccggaaatc ctttgtggtt aactgctcag gaaagccagc aaaagtcaga acaggcaaaa
     3361 gctgcccaga ctgtttccca gtcggcccag gaagaacctg cattccaggt tgtaaaaaac
     3421 atgccttctt ttaagggtgg aatggatgcc atgacaaaat acctcgtgga aaacattaaa
     3481 tatccacctg aagcaaaaac aaaaggaaca gcaggaaccg ttttcgttac ctacgttatt
     3541 gaaaaagatg ggtcggtgac caatgtcaag attctccagg gaattggcga cggatgtgat
     3601 gaagaagctg cacgggttgt caaaggtatg cctaaatgga acccgggcac aaatgacaat
     3661 aatgagccag tcagggtaat atttaacctc ccgataaagt tttccctgga aggtaaaaaa
     3721 gaagaagtaa aataattacc ttagcaaggt aaccatccct tcatagactt ttttcgaacc
     3781 gcgcaggttc ttcccgaaga tcctgaaaat atactgccct ccgggcattt caaccccgtt
     3841 cacatccctg ccgttccata aacattccgg gttgtgaacg ggttcatcca caagctgaac
     3901 accccaacgg ttataaatga tgatccggaa tacatcaacg atgtcgcccg ttgtaccgcc
     3961 aatggcaaag cagtcgttca atccatcgcc attcggtgta aaggcattgg gaacaaaaat
     4021 cttaatatcc tccacaatac caatctccag cgtatccctg tcccagcatc cgtgttcggt
     4081 ctcggtatac attacgacca ggtaagtgcc tggtaaagtg tatgtatgcc ttggatttgg
     4141 actggaaaga tcctcattgc cgtcgccaaa atcccagttg ataatctggt aggggatatt
     4201 gacctgttcc gagaaattga ttgtaggatt atcaatagta gccgcctgag gatcagctgt
     4261 aaaatcggct accggcacct caaacacctc aaccatatca ttaatggtca aagtattgac
     4321 acaacctgtc gaattggtca ccgttaaggt aatatcatac gtccccggta tggtgtattc
     4381 atgggaagga ttcacatcag tgcctgttgt gccatcgccg aagtcccaca ggtatgaagg
     4441 attttcaagg tttgatgaga tattggtgaa tgacacctga ataggctggc aaccggctgc
     4501 acctgttgcc gtaaaaccgg ccagaggatc gggatacacc tggtactgaa aatagaaagt
     4561 ctcttcgcaa cccagttctt caatgtgcag gctgatattt ttcaatccgt ctgtcgacca
     4621 gacgatctgc ggtggaacgg ccaggttact ggtagccggc actccatcat caaatgtcca
     4681 ggaataagta gcgtcgggta gtggtgtcac agtcggcagg aaagtgatcg tcaccggctt
     4741 gtcggtacac tggaacccgg gatcaccgag gatattgccc gccagccttg gcctgacatt
     4801 gaccaccacc gtggctgaat cgagacagcc atggttatcg gtgatcctgc acttaaatgt
     4861 tgtggtttcc tggggcgtaa ccgttggcga ggcctgggtt tgctgtcccg gctgccaggc
     4921 atacgcaggg tttgctgtcc agaaatagct ggaaatggtg accgggccgg tcggtacaat
     4981 gcttagatcg gtcggataat caatacagac cgattcgatc gaagtcgaag caacaggata
     5041 agcgtaggga tagactgtta ccgttcgggt aatagtgtca taacaaccgt gattattata
     5101 agctataact gttaccgggt gaggcccggc ttcggtcatt tccaggctga tattctgccc
     5161 gtaagcctca tagttcgtcc cgtcgtcctc ccaggtccag taccacccat caaaagcatg
     5221 aagttcctgc agcgtaaatg ggacgttcgg gcaggtgaag aaattggtcg aattgttcat
     5281 ggtcatatcc atcaccagtt ccgagacttc aaaagtgaaa ctggtgtcgg catactcatg
     5341 gcactcattc tcaattctta cccagatagt atggagtcct gagttcggag gggtataatt
     5401 caatacctgg ttctgtccga gcaagggacc agccaggctt ccgtaatgcc accaatactg
     5461 aagggcgaag ctgcacgtca gctgtatctc atcgtacagg cagatcagcg gcacaccact
     5521 gattgtgaca accggaaaga aggtagcatg cactgtatcg ttagccgagt ttccgcattc
     5581 atccgtcgct gtgcaaataa aagtaagggt attcggtgca aagctgccat agtcctccca
     5641 ggtaacggtg taaacagatt cattacccac ctgggtcaat acccctggag gattctcgaa
     5701 ataccagatg gtggaataat cgtcgccgac attggtactg acaatctcga tgtcggactc
     5761 tccgttctgg caaagctgaa aatctccgct ggcttcaatt tcgagcctgc tgtatataaa
     5821 aaccgtatca gaa
//
LOCUS       DAOQXS010000121         5833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14011_length_5833_cov_3.506231, whole genome shotgun sequence.
ACCESSION   DAOQXS010000121 DAOQXS010000000
VERSION     DAOQXS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14011_length_5833_cov_3.506231"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKM34_04715"
     CDS             complement(<1..729)
                     /locus_tag="PKM34_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNQ82922.1"
                     /translation="MVFDLNMIKGVYAGLAAKVQKARQLTGKPLTLTEKILYSHLSAE
                     NDPAPYYRGKSYVNFDPDRVAMQDATAQMALLQFMQAGKKRVAVPSTVHCDHLIQAKA
                     GAREDLMTALDINREVFDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGMPWELKFPKLIGVMLTGKLNGWASAKDVILK
                     VAGILTVKGGTGAILEYFGPGALDI"
     gene            complement(896..4078)
                     /locus_tag="PKM34_04720"
     CDS             complement(896..4078)
                     /locus_tag="PKM34_04720"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNQ82923.1"
                     /translation="MKRSVLVFLFFSLLGLYGYNQVFDERKNPKEMTPEEQQYLLERD
                     RPAKEFPVSPEGGTRAVGDDCTNPIIISIPAALPYNVTGQTNCGRGNTYSTSCLGNYD
                     GGEDIMYRLDVTATTLVTITMNPGTTTYTGMYLSATCGNSGTCIATSTGSGATPRVIS
                     NQTLTAGNSYYVMVDTWPTPNCIPSFSLNIVAVVANPVSFSASAFSSSQINLTFTPNA
                     SNHNVVIVWNNTGTFTTPAGTPPAAGQSFAGGTLLYNGTTSPQSHTGLNGGTIYYYKA
                     FSYDGSGYSSGLTANATTPCAAYSIPYTQDFNGSYLPTCWYEANGNAPNPTLSSSSWT
                     NSTYFANTTGNSAAVKMNLYGTDNDWLISPPIDLGSGSRRITLKVAGTTYNGTGSVTM
                     SADDTVFVLYSTNLTAPYNFVKANAITYYTNTHSPSVAGWDEVYELNGLSGNVLFAFY
                     AKGINNTPDMDFHIDDFVVEEVPACPKPTGLFTDNIGFTQADLDWVQSGSPAGWEIIW
                     GLPGFDPETEGTLITGVSSHPYTLNPPLDHSTIYQWYVKSDCGDGMLSGWSGPASFQT
                     LCQDITVYPYSQSFDGIAFPPICWDTSLIVTGTGSGTSGNWDRVTSGTFPTCSPFSGV
                     GMTKFASFNFKSGTKSIMVSPSLELPKDYYSVSFWMFRDNGYPTNNDMINIYYNNSPD
                     LNGAELLGSVSRYYLSSPVEATANLWYNYEFTLPEGSAGSGRYIIVEGVSAYGNNIFF
                     DEFIIREPSSLSGYVLDFDGNPVEGVLVEKVGGINTYSAADGSYELYPLPSGNQQFKC
                     SMTGNNDLFEYVDIPLYTTVNHNFTMLAPEINLSPGSFLFILGESETGSENLILTNTG
                     NGTLDWVLEELDGAPVDVPWLVADDYAGQLNPFGDSQTLLVTVDASLTPGSKAPGTTY
                     QAGLKFSSPSGIEPDTVPITLIVTDGGVKGPENLQVYVVSSNDGKFMLKWLYFTIKGL
                     NFEHFLILQNGQVIGTSEGSSFNVELDTPGMYCYKVFAVYSNGVYSNASNVACINYPL
                     PPNVPVSNWALLLAAGLMIIITIVYFRRRF"
     gene            4347..5756
                     /locus_tag="PKM34_04725"
     CDS             4347..5756
                     /locus_tag="PKM34_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ82924.1"
                     /translation="MVRPVKYKFREFLLDIKEAITGTERDFTSEKLGRAIFLLSVPMV
                     LEMAMESVFAIVDIYFVSRLGAETVAIVGITESIMTIVYALGVGLGTAATAIISRRIG
                     EKDPEHASQAAGQAIFVGVFISLMLAVPSVIFYRNILHMMGMDEQAINEYGSYTLIIL
                     GTNMMIMLLFINNAIFRSSGDPAISMKVLAVANGINIILDPILIFGFGPIPALGIKGA
                     AIATTIGRGLGILYQFYILFHGKHRIGLKVQHLLPDLKVILNLLKLASGAAGQHLIAT
                     SSWIFLVRVIALFGSPVVAGYTIAIRIMFFTLLPSLGVSNATATLVGQNLGAEKPERA
                     ERAVWITGYVNIVLLGLIGLFLALYPAPLIRLFIDDPQVIAYGTSCLRILSMGYIAYG
                     LGMVMVNALNGAGDTQTPTWINLFCYWLFEIPLAYFLAVNAGMGQNGVFIAILAAEIA
                     MTASALYFFRKGKWKLRKV"
BASE COUNT         1544 a         1332 c         1388 g         1569 t
ORIGIN      
        1 gatatccaag gcgcccgggc cgaagtattc caggatagcc ccggttccgc cttttacggt
       61 gaggatccct gcgactttca ggatgacatc ttttgcagaa gcccagccat tcagtttgcc
      121 ggtgagcatt accccgataa gtttagggaa tttcagttcc cagggcatgc cggccatgac
      181 gtcgaccgcg tcggcgccac caacacctat ggctaccata cccagaccgc ctgcattgac
      241 cgtgtgactg tcggttccga tcatcatccc tccgggaaag gcatagtttt ccagaaccac
      301 ctggtgtatg atgcccgcac cgggtttcca gaaaccgata ccgtatttat tgctaacgga
      361 tgacagaaag tcaaacactt cccgattaat atccagggca gtcatcagat cttcccttgc
      421 gccggcttta gcctgaatga gatggtcgca atggacggta ctgggaacag caaccctctt
      481 ttttccggct tgcataaact ggagcagggc catctgggcg gttgcatcct gcatagccac
      541 cctgtcagga tcaaaattga catacgactt gcccctgtaa taaggtgccg gatcattttc
      601 tgccgacaga tgggaataca ggatcttttc cgttaatgtt agcggtttgc cggtcaattg
      661 gcgggctttt tgaacttttg cagccaggcc ggcgtaaacg ccttttatca tatttaagtc
      721 gaataccata gaaatgctaa atattgaata ttgaaaaatt gaattttgaa tatttaattg
      781 attgtaaaag gagtgcaaaa atagtcaatt tagagaaaag aaaagccctg cagaatttga
      841 atcgctgcag ggctgaaaat cacttaatcc cggcaattaa cctaagaatt gttttttaga
      901 atcttcttct gaaatagact attgttatga tgatcataag tcctgctgcc agcagaagcg
      961 cccagttcga caccggcaca ttcggtggca aaggataatt gatgcatgcc acatttgagg
     1021 catttgaata aacaccattg gaataaacgg caaatacttt atagcaatac ataccgggag
     1081 tatccagttc cacattgaat gaactccctt cagatgtacc gataacctgg ccattctgca
     1141 aaataaggaa gtgttcgaaa tttagtcctt tgatggtgaa atacaaccac ttcaacataa
     1201 attttccatc attggatgaa acgacataga cctgaaggtt ttcaggacct ttaacgccgc
     1261 catcggtaac gatcaaagtg atgggtaccg tatccggttc aatgcctgag ggtgatgaga
     1321 atttcaatcc ggcctggtaa gttgttccag gggcttttga tcccggcgtg agcgatgcgt
     1381 caacagttac cagcagagtc tgactgtcac caaaaggatt taactggccg gcataatcat
     1441 cggcgacaag ccagggaaca tccaccgggg caccgtcgag ttcctcgagt acccaatcca
     1501 gggttccgtt gcctgtattg gtcaggatca gattttcgct tcctgtttca ctttctccaa
     1561 ggatgaacag gaaggagccc ggtgacagat taatttccgg agccagcatc gtaaaattgt
     1621 gatttactgt cgtataaagc ggtatgtcga catattcgaa gaggtcattg ttgccggtca
     1681 tggaacactt gaactgctgg ttaccggatg gcagcggata taattcgtag gatccgtcgg
     1741 cagcagaata ggtatttatt ccaccgactt tttccactaa aactccttca accggatttc
     1801 cgtcgaaatc aagcacatac ccgcttaaag atgaaggttc cctgataata aattcatcga
     1861 agaatatatt attcccataa gcgctgaccc cttctacaat tatatacctc cctgatccag
     1921 ccgatccttc cggaagggta aattcatagt tataccaaag gttggctgtg gcctcaacag
     1981 gtgatgacag gtaatatcga ctgaccgatc ctaataattc tgctccattc agatcaggtg
     2041 aattattata ataaatattg atcatgtcat tatttgtagg atacccattg tccctgaaca
     2101 tccagaaaga gacactgtaa taatctttgg gaagttcgag cgacggagaa accatgatag
     2161 atttagtacc gcttttaaag ttaaaactgg cgaatttagt cattccgact ccgctgaagg
     2221 gggagcaagt cgggaaagtg ccggaggtca cccggtccca gttcccggaa gttccactgc
     2281 cggtgcctgt cacaatgagg gaagtatccc agcagattgg ggggaatgca ataccatcaa
     2341 aactttggga ataaggatat acggtaatat cctggcagag cgtctggaaa gatgcagggc
     2401 ccgaccaacc gctcagcatc ccgtctccgc aatccgactt cacataccat tggtaaatag
     2461 tcgaatgatc gagcggagga tttaatgtat agggatgtga tgagacgccg gttatcaggg
     2521 ttccttcggt ttcagggtca aatcccggaa ggccccaaat aatctcccat cccgccggag
     2581 agccggattg aacccaatcc aggtcagcct gagtgaatcc aatattgtct gtaaacaatc
     2641 ctgttggttt gggacaagcc ggcacctctt ccaccacgaa atcatcgata tggaaatcca
     2701 tatcgggagt attgttgatt ccttttgcat aaaaggcgaa taaaacattg ccgctcagtc
     2761 cgttcagttc ataaacttca tcccatcctg ctactgatgg tgaatgggta ttggtataat
     2821 atgttatggc atttgcttta acaaaattat atggagctgt aagattcgtg gaatagagta
     2881 caaaaactgt atcatcggcc gacattgtta cggatcctgt gccattatat gttgtaccgg
     2941 caacctttaa agtgattcgt ctgctaccgg aacccaggtc aatcggcggc gaaataagcc
     3001 agtcgttgtc cgttccatag aggttcattt tcacagctgc tgaatttcct gttgtattgg
     3061 caaagtaagt ggagttagtc caagatgaac tgctaagggt tggatttggc gcatttccgt
     3121 ttgcttcgta ccagcaggta ggtaaataac tgccattaaa gtcctgggta taaggaattg
     3181 aatatgctgc gcagggcgtt gttgcattag ctgtcaaacc gcttgaatag ccagagccat
     3241 catatgaaaa agccttgtag taatagatgg ttccaccgtt taatccggta tggctttgag
     3301 gggatgtggt cccgttatat agcagtgtcc cgcctgcaaa gctctggcca gccgccggag
     3361 gagttccggc aggcgttgta aaagtgcccg tattattcca cacgatcacg acattgtgat
     3421 tggaagcatt gggtgtaaaa gtgagattga tctgggaact gctgaatgcg ctcgcgctga
     3481 atgaaaccgg attggcaacc actgccacaa tattcaatga gaatgaaggg atacagttgg
     3541 gggtcggcca ggtatccacc ataacataat aggagtttcc ggcagtgagc gtttgattgg
     3601 aaataacccg tggggtggcg ccgctaccgg ttgaggtggc gatacaggta cctgaattac
     3661 cgcatgttgc cgacaaatac atccctgtat aggtcgttgt acccgggttc attgtgatgg
     3721 taactaaagt agtggccgta acatccaggc ggtacatgat atcttcacct ccgtcataat
     3781 tgcccaggca ggatgtgctg tatgtattcc cgcggccgca attagtttgc ccggtaacat
     3841 tataaggtaa ggctgccgga atactgataa taatcgggtt ggtgcaatca tcgccgaccg
     3901 ctctggtgcc gccttccggc gatacgggaa actcctttgc gggccggtct ctttcaagga
     3961 gatactgctg ttcttccggg gtcatctcct ttggattctt tctttcatca aacacctggt
     4021 tgtaaccgta taaaccaagc aaactgaaga ataaaaaaac taaaactgat cttttcataa
     4081 catttaggtt taatctaata gtaagaggaa tgattgattg acgaatatcc gaaaatatga
     4141 atttacttgt tttaatgatt tggctataat ggtaaaaatc atgaataata tgatctgtac
     4201 cacttgtcaa atataagata ttctattgta taatacatct gcttatcctg ttctatgaaa
     4261 tttaaactca atcttaataa atggctggaa taattaggtt caatttttct cagaatgtaa
     4321 gctaaggttt atctttggcc cataaaatgg tacgtccagt caaatataaa ttcagggaat
     4381 tcctgctgga tataaaagaa gccataaccg gtaccgaacg cgattttacc agcgagaaac
     4441 ttggccgggc gatttttctt ttatctgttc ctatggtttt ggagatggcc atggaatcgg
     4501 tatttgccat tgtggatatc tattttgttt ccaggctcgg agctgaaacg gtagcaatag
     4561 tcgggattac cgagtcgatc atgaccatcg tttatgcgtt gggcgttggt ctgggcacag
     4621 cggcaacggc aatcatttcc cgaaggatcg gggaaaaaga ccctgaacat gcgtcgcagg
     4681 ctgccggaca ggccatattt gtgggcgtat ttatttcgtt gatgcttgct gttccgtccg
     4741 tgatctttta ccggaacata ctccatatga tgggaatgga tgagcaggct atcaacgaat
     4801 acgggagcta cacattgatc atcctgggca caaatatgat gatcatgctc ctgtttatta
     4861 ataatgccat attcagaagc tcgggtgatc cggccatttc tatgaaggtg ctggccgtag
     4921 ccaacgggat caatataatc ctggatccca ttctgatatt cggattcgga cccatcccgg
     4981 ctttgggaat caaaggagcc gccattgcaa ccacaatcgg aaggggatta ggcatcctgt
     5041 accagtttta catccttttt cacggaaaac accggatcgg cctgaaagtc caacatcttc
     5101 tccccgacct caaagtgatc ctgaacctgt taaagctggc ttcaggggct gccggacagc
     5161 atctgatcgc tacctccagc tggatattcc ttgtcagggt cattgccctg tttggaagcc
     5221 cggtagtagc cggttacact attgccatcc ggattatgtt cttcacgctg ttgccctccc
     5281 tgggcgtcag caatgccacg gctacgcttg taggacagaa ccttggcgct gaaaaaccgg
     5341 aacgggcgga gcgggccgtc tggatcacag gttatgtgaa cattgtcctg ctgggtctca
     5401 tcgggctttt cctggcccta tacccagccc cgcttatccg tttgttcatc gacgatccgc
     5461 aggtgattgc ctacgggaca tcctgcctgc gaattctcag tatgggttat atcgcatacg
     5521 gactcggaat ggtcatggtc aacgcgctga atggcgccgg tgatacccag accccaacat
     5581 ggatcaatct gttctgctac tggctattcg aaatcccgct ggcctatttc ctcgccgtca
     5641 atgcagggat ggggcaaaac ggcgttttta tagccatcct cgctgccgag attgcaatga
     5701 cagccagtgc actgtatttt ttcaggaaag gcaaatggaa gctccggaaa gtttagcatt
     5761 ccgggataaa atgatactta ttgagatacg ctcataagca cttcttaata cgctgataag
     5821 tccgttcgat atc
//
LOCUS       DAOQXS010000122         5832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14015_length_5832_cov_3.656223, whole genome shotgun sequence.
ACCESSION   DAOQXS010000122 DAOQXS010000000
VERSION     DAOQXS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14015_length_5832_cov_3.656223"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1777)
                     /locus_tag="PKM34_04730"
     CDS             complement(89..1777)
                     /locus_tag="PKM34_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNQ82925.1"
                     /translation="MRKIILLLISINLLHCVSFGQSIYELEGLVVDENNQPLSGATII
                     LFPGEKATTTGFQGNFTISGLEQGTYRIEISFIGYKPLQDTVTMNGNTTYRAQLSLSP
                     LTLHEVVISDNYSEERKKEEPLNIEIVNDRYLRQNLGGSVMQSLERLPGLSTIDIGPG
                     QSKPVIRGLGFNRVVVLENDIRHEGQQWGADHGLEIDQYAVGRVEVVKGPASILYGSD
                     AIGGVIDLQTNHIPAENTVGGSVDISGKSNNDFGGGSVMLYARKHALHANLRVTLLGF
                     ADYRVPADSIDIYSYRAALDRRRMRNTAGNEQSVNLSVGYLGTKFRSRLFISDLNGKT
                     GFFANAHGLEPRNVDTGLHDASNRDINMPYHYVNHLKIINQSRLDLEKVTVEAGLGFQ
                     RNFIQEWSRYIAHGYMPAAFPGNTGYSADLEREFKKYIYSGNLKIRYDRNDRSEFDFG
                     LNSEFHDNRIGGWAFIIPAYKQFTIGGFALGKYSFSQKSILQAAVRYDYGHIRTAEYR
                     DWYPSPVINESDTAYEHLQRAAAIRPIRVRPDRAPTSPSSRPANATPRRGRWRR"
     gene            complement(2025..2384)
                     /locus_tag="PKM34_04735"
     CDS             complement(2025..2384)
                     /locus_tag="PKM34_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82926.1"
                     /translation="MKPFRLYIALILLGIFLFPVLFQSVHSTGHKKKCCSGYENFCES
                     VLPHKDLHTNSLAEQSKEKKCPVCKYQFPVNKLPELSLYSTIAPVITGTICEIILSAD
                     INDAFSIKSPRAPPAIS"
     gene            complement(2381..2797)
                     /locus_tag="PKM34_04740"
     CDS             complement(2381..2797)
                     /locus_tag="PKM34_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ82927.1"
                     /translation="MKASEVLYSRQLKRTSCREGIIDVMMAAGRALSENEIRERLPGN
                     YDRTTFYRSFKTLEEYKIIHKIIIDNQLSKYSLDNSVTHKSEHAHFYCNDCQTVTCLY
                     SIPVQEVRLPNGFTIAEKEILIKGTCPDCKNIQTRI"
     assembly_gap    2937..3036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3104..3604)
                     /locus_tag="PKM34_04745"
     CDS             complement(3104..3604)
                     /locus_tag="PKM34_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ82928.1"
                     /translation="MMEGVISHVVFTVKNSRYDLQRIKRKKSVGLISPTFPFFENEQE
                     DLIIKILCDVASELGLNIKAFNFCGDHVHTIVVSDNPDISKKIGLWKGKSAYLYNVQS
                     QSRNPVKITQNLWAKSYYQKTITSKNEQEKQISYINNNRKKHGLAPLSKMSANIVHAL
                     IQDKDE"
     gene            3856..4278
                     /locus_tag="PKM34_04750"
     CDS             3856..4278
                     /locus_tag="PKM34_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ82929.1"
                     /translation="MTIQDCIKFTNENPICYLATVENDQPRVRIIHFWFADESGFYFQ
                     TGSIKEMYRQLKANPKMEACFYRHEGMLGTMLRIAGEAEFLNDKQMKEKAMADRPFLK
                     EFGLSADSPHLIIFRIAHGRACFWTKERNLLPKEYIEF"
     gene            4306..5040
                     /locus_tag="PKM34_04755"
     CDS             4306..5040
                     /locus_tag="PKM34_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ82930.1"
                     /translation="MKTWKTKSGYTITKVLAGRSNAFLVSNGIVNVMVDTGPARNRKR
                     LQRNLNRLHIEKIDLLLLTHTHYDHAGNAGQIKRTYNATVAVHAREKSFLETGHNILP
                     KGTIPITKLLMNLLGKRLSGRFTYDPCQPDILIEAVRPLNDFGLSACVLPTPGHSPGS
                     VSLIVDDEIAIVGDAMFGIFKGSIFPPFADDTAMMIRSWKVLLDTGCRTFLPAHGFEN
                     SRGKVEKNLRDAIPRDGVPKDGVPAG"
     gene            5125..>5832
                     /locus_tag="PKM34_04760"
     CDS             5125..>5832
                     /locus_tag="PKM34_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNQ82931.1"
                     /translation="MKNLNKFIFGTVEKVGNALPNPAALFGLFALITLLLSALGYWLN
                     WHGINPSTGETIRVTNLLTHDGLHRILLEMVTNYTSFAPLGIVMVALLGIGIAESSGL
                     VKAAINALILRAPKNSITFMVVFTGVMSNAASDLGYILIIPLAGMIFHSLGRHPVAGM
                     SAAFAGVSGGFSANLVIGTIDPLLAGLSTEAARIIDPEYYVMPTANYYFMAASTFLVS
                     IFGTLVTRYIVEPRLGKY"
BASE COUNT         1398 a         1368 c         1425 g         1541 t
ORIGIN      
        1 atggaagacc gcacccgcga gttccgccgc cacgtgcgcc agagctacct cgaccaggcc
       61 caggccgacc cggcccattt ccgcgtgatc aacgccgcca acggccccga cgaggtgtgg
      121 cgttcgctgg tcgagacgat ggagaagtgg gcgctcggtc tggccgcacc cggatcggcc
      181 tgatcgccgc ggcccgctgc aggtgctcat acgccgtatc actttcgttt atcaccggcg
      241 acgggtacca gtcgcggtac tccgctgtac ggatatggcc gtagtcgtac cggaccgccg
      301 cctgcaggat gctcttctgc gaaaacgagt actttccgag cgcaaagcca ccaatcgtga
      361 attgcttata ggcggggatg atgaatgccc atcccccgat ccggttgtcg tggaattcgc
      421 tgttcagccc gaaatcgaat tcggatctgt cgttccggtc gtacctgatc ttcaggttcc
      481 cggaatagat gtacttctta aattcccgct cgaggtcggc gctgtagcct gtgttaccgg
      541 ggaaagccgc cggcatgtag ccatgggcaa tatatctgct ccattcctgt atgaaattgc
      601 gctgaaagcc caggccggcc tccaccgtta ccttttccag gtcaagccgg ctctgattga
      661 tgatcttgag atggtttacg taatgatagg gcatgttgat atcgcggttc gacgcgtcgt
      721 gcagtccggt atcgacattg cgcggttcca gcccgtgcgc gttggcaaag aacccggtct
      781 ttccgttcag gtcgctgata aagaggcggc tccggaattt agtccccagg taaccgacgg
      841 agaggttcac actctgctcg ttgccggcgg tgttgcgcat acggcgacgg tcgagggcgg
      901 cacggtagga gtagatatcg atactgtcgg ccggtacgcg gtagtcggca aagcccagga
      961 gcgtcacgcg gaggttggcg tggagcgcat gcttccgtgc gtacagcatg acggatccac
     1021 cgccgaagtc gttgttgctc ttgccggaga tatcaacgct gcctccgacg gtgttttcgg
     1081 cggggatgtg gtttgtttgc agatcgatca caccgccgat ggcatccgaa ccatacagga
     1141 tcgacgcggg gcctttcacc acctccaccc ggccgacggc atactggtcg atctccagcc
     1201 cgtggtcggc gccccactgc tgcccttcgt gccggatatc gttttccaat accaccacac
     1261 ggttaaagcc caggccccgg atcaccggtt tggattgccc cggtccgatg tcgatcgtgg
     1321 agaggcccgg caggcgttcg agcgactgca tgacgctgcc gcccaggttt tgcctgaggt
     1381 accggtcgtt cacgatttcg atgttcagcg gctcttcctt tttgcgttct tcggaatagt
     1441 tatcgctgat aaccacttca tgcagagtaa gagggcttaa ggaaagctgt gcccggtaag
     1501 tcgtattccc gttcatggtc acggtatcct gcaaaggctt gtacccgata aaagatattt
     1561 caatccggta ggtgccctgc tccaggccgg atatcgtgaa atttccttga aaaccggtcg
     1621 tggtggcttt ttctcccggg aacaggatta tagtggctcc tgacaagggc tggttgtttt
     1681 cgtcgacaac caagccttcc agttcataaa tactttgccc gaacgaaaca cagtgcaaaa
     1741 gatttatgct tatcagaagc agaattattt ttctcacatt aaatgaattg aaaatgaact
     1801 tcaactgtaa aaagaggatg tttcaaaaag ttagatttct ttcaaaataa cttcgtggat
     1861 cttcgtgcct tcttcgtgat cctctgtgta acaatcattt ataagttcca cgaaggtttc
     1921 acgaaggttt cacgaaggtt tcacgaagct acacgaaggg gttatgaaac aacctcttag
     1981 ataaaatacc cgttccgtaa ggcggggatt tgaccttgag gagattagct gatggcaggc
     2041 ggggctctgg gtgatttgat agaaaaagca tcatttatgt ctgctgataa aatgatttcg
     2101 cagattgttc cggtgataac cggggctatc gttgaataaa gtgataattc gggcagttta
     2161 ttcaccggga actggtattt acacaccggg catttctttt cctttgattg ttcagcaagg
     2221 gaattggtat gcagatcctt atgcggtaaa acagactcac agaagttttc atagcctgaa
     2281 cagcatttct ttttatggcc ggttgaatga accgattgga acaggaccgg gaaaagaaat
     2341 attcccagca agatcagggc tatatagagt cggaatggct tcaaatgcgt gtctggatat
     2401 ttttacagtc agggcaggta cctttgatca ggatctcttt ttcggcaatg gtaaagccgt
     2461 tgggaagccg gacctcctgg acggggatgg aatacaggca tgtaacggtc tgacaatcat
     2521 tacagtagaa atgagcatgt tcacttttat gggtcacaga gttatccagt gagtatttac
     2581 tcaactgatt atcaataatt atcttatgga tgattttata ttcttccagc gttttaaagg
     2641 agcggtagaa tgtagtgcgg tcgtagttac ccggaaggcg ttctctgatt tcattttccg
     2701 acagggcgcg gcctgcggcc atcatcacgt cgatgatccc ttcgcggcag cttgtccgct
     2761 tcagttggcg ggagtacaga acttcagaag ctttcatgga tgcaaatata tttcaaatgc
     2821 aacaatgttg caaagatatt aagttttttg tttgataggg tttcttcaaa cgactagata
     2881 ggttttcttc cgacgactga agccgtcggt tcctttaatg ttttcttccg acgactnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttt tcttccgacg actgaagccg
     3061 tcggttcctt taatgttttc ttccgacgac taaagccgtc ggttcattcg tctttatctt
     3121 gaatgagagc atggacaata ttggctgaca tttttgacaa aggggccaga ccatgttttt
     3181 ttctattatt atttatgtaa gagatttgtt tttcttgctc attttttgat gtaatggttt
     3241 tttgataata actcttagcc cagaggtttt gtgttatttt aacaggattc ctggattgag
     3301 attgaacatt atataaatag gcagatttgc ctttccaaag tccgattttt ttggatatat
     3361 ccggattatc cgatactacg attgtatgaa catgatctcc gcaaaagtta aatgctttta
     3421 tatttagacc taattctgat gcaacatcac acaatatttt aattattaga tcttcctgtt
     3481 cattctcaaa aaagggaaat gtcggtgaaa ttaatccaac agatttcttc cgtttgattc
     3541 gttgcaaatc ataccgggaa tttttgacag taaaaacaac atgagaaatc accccttcca
     3601 tcatccgaca caattattat taataatgaa aactcaaaaa tagcataaat cactgaaccg
     3661 acggctttag tcgtcggaag agatcttctc aggaaccgac ggctttagtc gtcggaagag
     3721 aacttaattt attaaatatc gcctctataa tgcggcatct tctcttttct ttatttttgc
     3781 ctggacctga aaaacataga caggagaaga catccagtca taaaacattt tatatcaata
     3841 tctagaaaaa ttatcatgac tattcaggat tgtattaaat tcaccaacga gaaccctatc
     3901 tgctacttag ccactgttga aaacgaccag ccacgcgtcc gcatcatcca tttctggttt
     3961 gccgatgaaa gcggttttta ttttcagacc ggttcgataa aagagatgta ccggcaactg
     4021 aaagccaatc ctaaaatgga agcatgcttt tacaggcacg agggcatgct cgggaccatg
     4081 cttcggattg ccggcgaggc ggagttcctt aacgataagc agatgaaaga gaaagcgatg
     4141 gccgaccggc cttttctgaa ggagttcgga ctgagtgccg acagcccgca cctgatcatt
     4201 ttccgcatcg cccatggccg ggcctgtttc tggacgaagg agcggaacct gttgccgaag
     4261 gaatatattg aattttagag aaagggacgc catccctttg accggatgaa aacctggaaa
     4321 acaaaaagcg gatacaccat taccaaagtt ttagccggac ggagcaatgc ttttcttgtt
     4381 tcaaacggta ttgtcaatgt aatggtcgat accggcccgg caagaaaccg gaaaaggctg
     4441 caaagaaatt taaaccggct tcacatagaa aagattgatt tacttcttct tacccatact
     4501 cattatgacc atgcggggaa tgcaggccag atcaaaagaa cttataatgc aactgtagcg
     4561 gtgcatgccc gggaaaagtc ttttcttgaa accggccata acatcctgcc aaaagggacc
     4621 attccgatta caaaattatt gatgaacctt ctgggtaaac ggttatccgg aagattcaca
     4681 tatgaccctt gtcagcctga tattttgatt gaggccgttc gtccgctgaa cgattttggt
     4741 ttgagtgcct gtgttctgcc cactccggga cattcccccg gatcggtgag ccttattgtg
     4801 gatgatgaga tcgccattgt gggcgacgcc atgttcggaa tattcaaagg ctcaatcttt
     4861 cctccctttg ccgatgatac ggctatgatg atcaggagct ggaaagtgtt gctggatacc
     4921 ggatgtcgca cctttctgcc ggcgcatggc tttgaaaaca gccgggggaa agtggagaag
     4981 aacttaaggg acgccatccc gagggatggc gtcccgaagg atggcgtccc tgcgggctaa
     5041 acaaaaaggc ggaaagagtg ttaattcttt ctataaacag attaacattc taaagatctt
     5101 accctcaatc agtcctgatg cacgatgaag aacctcaaca aattcatttt cggtacggtg
     5161 gagaaggttg gcaatgcatt gccgaaccct gctgcattgt tcggcctgtt tgctttaatc
     5221 accctgctgt tatcggcttt agggtactgg ttgaactggc acggcatcaa tccatccacc
     5281 ggtgaaacga tccgtgttac caacctcctt acccatgacg ggttgcaccg gatcctgctg
     5341 gagatggtga ccaattatac aagctttgcc ccgctgggca tcgtgatggt ggctttgctg
     5401 gggatcggta ttgccgagag cagcggacta gtgaaggcag ctattaatgc cttgatcctg
     5461 agggctccca aaaattccat cacctttatg gtcgtattta ccggtgttat gtcgaatgcc
     5521 gcttccgacc tgggctacat cctcatcatc ccactggccg ggatgatctt tcattccctg
     5581 ggccggcacc cggtagcagg catgtcggca gcttttgccg gggtcagcgg aggattcagc
     5641 gccaacctgg tgatcggcac catcgatccg ctcctggccg gcctctccac ggaagcagcc
     5701 cgtatcatcg acccggagta ttacgtgatg ccgacggcca actactattt catggcggcg
     5761 tcgacattcc tggtctccat tttcggcaca ctggtcaccc gctatattgt agaaccgcga
     5821 ctgggaaaat at
//
LOCUS       DAOQXS010000123         5826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14039_length_5826_cov_3.750477, whole genome shotgun sequence.
ACCESSION   DAOQXS010000123 DAOQXS010000000
VERSION     DAOQXS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14039_length_5826_cov_3.750477"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKM34_04765"
     CDS             complement(<1..408)
                     /locus_tag="PKM34_04765"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HNQ82932.1"
                     /translation="MEKILFVLIIAIVVFEFLLERVLDYLNATCWSEKLPEELQGIYE
                     QEKYSRSQQYLKANHWFSQLTESFNFILVMLMLLLGGFAFLDNQVRAITDNPVLMALL
                     FFGAIGVVADFLGTPFSIYSTFVIEQRFGFNKTT"
     gene            complement(422..2527)
                     /locus_tag="PKM34_04770"
     CDS             complement(422..2527)
                     /locus_tag="PKM34_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ82933.1"
                     /translation="MPELFTGQPSWLIIVCLALAFGYATSLYFRDRKNEFPGYLKTIL
                     GIVRFLAIFFISFLLLAPFVKTTSKEKEKPLLIFALDNSESVIIHKDSLAVKSELPAI
                     LDNLADKLTERGEAQKYYFDEKVYRLDGSADYNSSAAFNGKATDIANAVGEIVNIYTN
                     RNIGALILVSDGIFNTGTNPLYRAKDIPFPVYTVALGDTSIRKDLVVAGVNYNRMVYL
                     NNKFPLELVVRAGNAAGNQSRLRIVQQDNVLFSQDFQVNDDDFTQTFRIVLEAKRKGL
                     QKFTVIIDPIDGELSYSNNRKDIFVEVLDAKSRVLILSGAPHPDISALRQAILSNMNY
                     EVDVSTLNDFTGKLDSYSLVILHQLPSVNFPAENITREITEKAIPVLFILGKQSDLAR
                     VNQSKPGLSIVTARPSFEEAVPKYNPAFSAFSLSESTVSWLSDLPPLVAPLGDYQVAN
                     AARVLVSQRIGSVETTRPLILLNETLEGRKGIIAGEGLWKWRLFNFARFQNHQSFNEL
                     INKLVQYLSLKDQKKNFRVYQPGSFRETTPVIFDAEVYNDNYELTTLPEVQLSIINEE
                     GKTFPYVFNKTGNSYHLDAGAFPPGNYSFRAETSLDGRTLYETGEFSVLSLDLEALNT
                     VADHNLLYQISSETGGEMFYPADLDKLASAILEREDIRPLTYTGKKYEDLLNKSWILL
                     LILGLLTLEWFVRKRAGSY"
     gene            complement(2532..3275)
                     /gene="fabG"
                     /locus_tag="PKM34_04775"
     CDS             complement(2532..3275)
                     /gene="fabG"
                     /locus_tag="PKM34_04775"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNQ82934.1"
                     /translation="MLLKDKTALITGAARGIGRAIALQFAAQGADVAFTDLRADENME
                     SLEKEIAAMGRKGKGYTSDASDFNGAEAVVDQIVNDFGKIDVLVNNAGITRDNLLMRM
                     TEADWDLVIKVNLKSVFAMTRAVQKYMLKQRFGSIINMSSVVGVSGNAGQSNYSASKA
                     GLIGFTKSIAQELGSRNIRCNAIAPGFIETEMTAKLPQDVREAWIKDIPLKRGGKPED
                     VAHIALFLASDLSDYVTGQVINVCGGMNT"
     gene            complement(3325..5823)
                     /gene="glgP"
                     /locus_tag="PKM34_04780"
     CDS             complement(3325..5823)
                     /gene="glgP"
                     /locus_tag="PKM34_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNQ82935.1"
                     /translation="MPKGLEKLSRMFYNLWWTWNHEAEELFEQIDPVLWKQSGRNPRI
                     LAELLNINRLKSLEKDSAFIQKVDAVYAKFQDYMALAENKPKEKIAYFSMEFGLDSNI
                     KTYSGGLGILAGDYLKEASDSNKNMVGVGLLYRYGYFQQSISLFGDQIAEYHRQKFSH
                     LPLSRVFNGNGELMKIRIALPGRTLFAQVWRVKVGRIMLYLLDTDISDNSLADRNVTH
                     QLYGGDWENRFKQELLLGVGGIRALNELGIEADVYHINEGHAAFLNLERLRILVQEKG
                     LTFYQALEVVRASSLFTTHTPVPAGHDFFDENLLRTYISHYPERLNISWQDFMNLGKF
                     RENDPGEKFSMSVLAVKMSQEVNGVSKIHGRVSREMFHGLYEGYYADELHIGYVTNGV
                     HHPTWTGSHWIDLYRKHFGEEYLLHQSDASYWEKIRQVPDAEIWKLRNHYRRDLIDYL
                     RERLTDELTKRQENPMLKLKMVDALDENALTICFSRRFATYKRAHLLFSNPERLSALL
                     NNKQFPIQIIYAGKAHPADGAGQDLIKRIVEMSRKPEFLGKIFFIENYDMELAKKLVR
                     GVDIWLNTPTRPLEASGTSGEKAVMNGVLNLSVLDGWWAEGYRPHAGWALREARTYVN
                     QQFQDELDSETIYDLLEDEILPVFYERDNGIPKKWVSYIKNCISQIAPHYTMKRMLDD
                     YQHKYYDRLIRRSKDIRSDDYQLARDIEAWKSKMRAGWNHIEVRRIAVPDPARKSLDL
                     GDNFIAEIELKLNGIKGEDLGMDILFGQKEMDTVNKIMHKEEMKMVDSAPGVAKFTCK
                     IPMEKIGVYHYAFRLYPKKEWLAHKQDFNLVKWL"
BASE COUNT         1405 a         1433 c         1404 g         1584 t
ORIGIN      
        1 tgtggtttta ttgaatccga atctttgttc gattacaaag gttgaataga tggaaaaagg
       61 ggttcccagg aaatcagcca ccacccctat cgcaccaaaa aacaaaagcg ccatcaacac
      121 cgggttatct gttatcgccc tgacctggtt gtcgagaaaa gcaaatcctc ccaatagaag
      181 catcagcatg accaggataa aattgaagga ttcagtaagc tgtgagaacc aatggtttgc
      241 cttcaggtac tgttgggacc gcgaatattt ctcctgttca taaataccct gcagttcctc
      301 cggcagtttt tcgctccagc acgttgcgtt caggtaatcc agcactcttt cgaggaggaa
      361 ctcaaagaca acaatagcaa tgatgaggac aaacaggatc ttttccataa ataatatagg
      421 atcaataact tccggcccgt tttctgacga accactctaa tgtcagcaat cccaggatca
      481 ggaggagtat ccagctttta ttcagcaggt cttcatattt cttgccggtg tatgtcagcg
      541 ggcggatatc ctccctctca agtattgcgg atgccagttt atccagatca gccggataga
      601 acatttcccc tcctgtttca ctggaaatct ggtacagcag gttatggtcg gccacggtat
      661 tcagggcttc gaggtcaaga gagagcacag aaaattcacc ggtctcataa agtgttcgtc
      721 cgtcgaggct ggtttcggca cggaaggaat aattgcccgg cgggaacgcg ccggcatcca
      781 ggtggtagga attgccggtt ttattgaata catagggaaa tgttttacct tcttcattta
      841 ttatacttaa ctgaacctcc ggaagtgtag ttaattcata gttatcatta tagacttctg
      901 catcgaatat aacgggggtt gtctcgcgaa aactgcccgg ttggtagacc ctgaagtttt
      961 tcttctggtc tttcaggcta aggtattgta ccagcttatt tatcaattcg ttaaacgatt
     1021 gatggttttg aaaacgggca aaattaaaga gtctccattt ccagagtcct tcgccggcaa
     1081 tgatgccttt acgcccttcg agggtttcgt tgaggaggat gagcgggcgg gtggtttcga
     1141 cgcttccgat acgctggctg acaagcaccc gggcggcgtt ggccacctgg taatcgccca
     1201 ggggggccac caggggcggc aggtcggaaa gccaggaaac ggtgctctca ctcagcgaaa
     1261 aagcggaaaa ggccggatta tacttcggta cggcctcttc gaacgacggc cgggcggtta
     1321 cgatgcttaa ccccggcttc gactggttaa cccgggccag gtcagactgt tttccaagta
     1381 taaacaggac cgggatggcc ttttcggtta tttcccgggt gatattttcg gcagggaaat
     1441 tcactgaagg cagttgatgc aggattacga ggctgtaact atccagcttt cctgtaaaat
     1501 cattcagcgt ggaaacatca acctcataat tcatatttga taatatcgcc tggcgcagcg
     1561 cgcttatatc gggatgcggt gctcctgaaa ggatgagtac gcggctcttt gcatcgagca
     1621 cctccacaaa aatatctttt cggttattgg aatacgacaa ttccccgtcg atcggatcga
     1681 tgatgacggt aaatttctga agtccttttc ttttagcttc cagcacgatc ctgaatgtct
     1741 gagtaaaatc atcatcatta acctgaaagt cctgggagaa caggacatta tcctgctgga
     1801 cgatcctcag cctgctttgg ttaccggcgg cattgccggc tctgacaact agttccagcg
     1861 ggaacttatt attcaggtaa accatccggt tataattcac gcctgcaacc accaggtctt
     1921 tccggatgct ggtatcgccc agggcgactg tataaaccgg aaaaggtata tctttcgccc
     1981 ggtatagtgg attggtcccg gtattgaata tcccgtcgga tacaaggatc agcgctccta
     2041 tgtttctatt ggtgtatata ttaacgattt caccgactgc attggctata tccgtcgctt
     2101 tgccattaaa ggctgcggaa ctgttgtaat ctgcactgcc atccagccgg tacactttct
     2161 catcaaaata gtatttctgg gcttcgcccc tttcggtgag tttatcggcc aggttatcca
     2221 gaatagccgg taattcactt ttaaccgcaa gggaatcttt gtggatgatc accgattctg
     2281 agttatccag ggcgaaaatc agcaaaggtt tttccttctc ctttgaagtg gtcttgacaa
     2341 acggtgccaa taacaggaat gaaataaaaa agatggccaa aaagcggaca atgcccagga
     2401 tggttttaag ataaccgggg aactcatttt tccggtcgcg gaaatagagg ctcgtggcat
     2461 agccgaatgc cagcgccagg cagacaatga tcagccagga aggttgtccg gtaaagagtt
     2521 cgggcatcag atcaggtatt cattcctccg cagacgttga tcacctggcc ggtgacataa
     2581 tccgacaggt cggaggcaag gaacagggct atgtgggcta catcctcggg tttgcctccc
     2641 cttttcaacg gaatatcttt aatccatgcc tccctgacat cctgcggaag ctttgccgtc
     2701 atttccgttt cgataaatcc gggggcaatg gcattacacc ggatattacg ggagccgagt
     2761 tcctgtgcga tggatttggt aaatccgatc aggcccgctt tggaagcaga ataattggat
     2821 tgccctgcat taccgctgac accgacgacg gagctcatat tgataatgga tccgaagcgc
     2881 tgtttgagca tgtatttctg cacggcccgg gtcatggcga agaccgattt caggttgact
     2941 ttgatcacca ggtcccagtc ggcttcggtc attcgcatca gcaggttatc ccgggtaatt
     3001 cccgcattat tgacgagaac atcaatttta ccgaagtcgt tcacgatctg gtcgacaaca
     3061 gcttcagctc cattgaaatc actggcatcg gaagtatacc ctttgccttt cctgcccatt
     3121 gcggcaatct ctttttcaag gctttccata ttttcatcgg cccgcagatc ggtaaaagca
     3181 acatcagcgc cctgagcggc aaattgaagc gcgatggccc gtccaatccc acgggcagcg
     3241 cctgttatga gagcggtctt atctttcagc agcataggtc aatttttttc aaatttacga
     3301 ataaaaaagc taaaaatcat cgggctacag ccacttgacc aggttaaaat cctgtttatg
     3361 tgcgagccat tccttttttg gatataacct gaacgcataa tggtacacgc ctattttctc
     3421 cattgggatc ttacaggtaa attttgcgac gccgggggcc gaatccacca tcttcatctc
     3481 ttctttatgc ataatcttat taacggtatc catctccttt tgtccgaaaa ggatatccat
     3541 acccaggtct tcacctttga ttccgttgag tttcagttct atctccgcaa taaaattatc
     3601 gcccaggtcg agggatttgc gggccgggtc cggtacggct atccgcctga cttctatatg
     3661 gttccatccg gccctcattt ttgacttcca cgcttcgata tcacgggcaa gctgataatc
     3721 atcactccgg atatctttcg aacgcctgat cagtctgtca taatatttgt gctggtaatc
     3781 atcgagcatt cttttcatcg tataatgcgg ggcgatctgg gaaatacagt tctttatata
     3841 agacacccac tttttgggaa tcccgttatc tctttcgtaa aacaccggca gaatctcatc
     3901 ttccaggaga tcatagattg tttcggaatc gagctcatcc tggaattgct ggttgacgta
     3961 ggtcctggct tcccgcaagg cccaaccggc gtgaggcctg tagccttcag cccaccagcc
     4021 gtcgagcacg ctaaggttaa gcacaccgtt cattacggct ttttccccgc tggtcccgga
     4081 tgcttccagg ggacgtgtgg gggtattcag ccagatatcg acaccgcgga cgagcttctt
     4141 ggccagttcc atgtcgtagt tttcgataaa aaatatcttt ccgaggaatt cgggctttct
     4201 tgacatctcc acaatgcgct tgatcaggtc ctggccggcg ccgtcagccg ggtgagcttt
     4261 tccggcataa ataatctgga tggggaattg tttattgttc agcagcgccg acagcctttc
     4321 cgggttactg aaaagcaggt gagctctttt ataagtcgcg aaccggcggc taaaacaaat
     4381 cgtcagggca ttttcgtcga gcgcatcgac cattttgagc ttaagcatcg gattctcctg
     4441 ccttttagta agctcgtctg tgagccgctc tctcaggtaa tcgatgagat ctctgcgata
     4501 atgattgcgc agtttccaga tttcagcgtc cggcacctgc ctgatttttt cccagtaaga
     4561 tgcatccgac tggtgcagca ggtattcttc tccgaagtgc ttcctgtaca ggtcaatcca
     4621 gtggctaccg gtccaggtcg gatggtgtac gccattggtc acatacccga tatgcagttc
     4681 atcggcataa tatccttcat aaagcccgtg aaacatttcc ctggagaccc gaccatggat
     4741 cttgctgaca ccattgactt cctgtgacat tttaacggcc agaacgctca tggagaattt
     4801 ttccccgggg tcattttccc ggaatttgcc caggttcata aaatcctgcc aggagatgtt
     4861 caggcgttcg ggataatggg agatataggt acgaagaagg ttctcgtcga agaaatcatg
     4921 tccggcggga acaggggtat gagtggtaaa aagagatgaa gcgcgcacca cttcgagtgc
     4981 ctggtagaaa gtcagcccct tttcctggac caggatgcgg agccgttcga ggttcaggaa
     5041 ggcggcatgg ccttcgttaa tatggtaaac atcggcctcg attcccagtt cattcagcgc
     5101 cctgatccct ccgaccccca gcaggagctc ctgtttgaac cggttttccc agtcgccgcc
     5161 gtaaagctga tgcgttacat tcctgtcggc cagtgaatta tcggagatat ctgtatccag
     5221 caaataaagc ataatccgtc cgactttgac tctccatacc tgggcaaaaa gcgtacggcc
     5281 gggaagcgcg attcgtattt tcattagttc cccattgccg ttaaacacgc ggctgagggg
     5341 gaggtgggaa aatttctgac ggtggtattc ggcaatctgg tctccgaaaa gggaaatgct
     5401 ttgctgaaaa tacccataac ggtataacag cccgacaccg accatatttt tattggaatc
     5461 gctggcctct ttcagatagt caccggccag gattcccagg ccgcccgaat aggtcttgat
     5521 attgctgtcg aggccgaact ccatgctgaa ataagctatt ttttctttag gcttattttc
     5581 agccagggcc atataatcct ggaactttgc atagactgca tccacttttt gaatgaaagc
     5641 cgaatctttt tccagtgatt tcaacctgtt gatattgagt aattcggcta gtatcctggg
     5701 atttcgtccc gattgtttcc agagtaccgg atcgatctgt tcaaagagtt cctctgcttc
     5761 atggttccac gtccaccaga ggttgtagaa catcctggaa agtttttcca gtcctttcgg
     5821 gatgga
//
LOCUS       DAOQXS010000124         5819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14068_length_5819_cov_3.784525, whole genome shotgun sequence.
ACCESSION   DAOQXS010000124 DAOQXS010000000
VERSION     DAOQXS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_5819_cov_3.784525"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKM34_04785"
     CDS             complement(<1..1421)
                     /locus_tag="PKM34_04785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ82936.1"
                     /translation="MTHRKMAFLRKTYRPWTILWVIQRFIVVMKERRLPQVRMVDLMY
                     DFRLTRKIRGDVRNRTWNTGLADPDVAGGLFNFVPTLEYKPFIINDDVDENELSVINH
                     IFDRIYVINLGRRLDRRVEMIMKLRKYCIKAEIFEAGNGYSKENSEAFAAYYDLPLAA
                     EGCHPLEKELKKKMIASPGSWGYLLTWKYLLNDAKSKGFARILVFDDDVLFHKDFEKK
                     TGESLKSIPGNWKLLYLGATQHDWSVPYNVNYPDKVGEKDFGVHPFYYPVKTDGSFAV
                     GVDCSVFDEILSEIEPMNCALDSGPLRAIQQRYPGQCFVVKPNLVVADVGQSDIGVER
                     DQGQMARKLRWQMDLYDYPFRKELVSVIMPAYNAEKTIEKSIRSILMQTYRELEMIVA
                     DDGSTDSTVAIVDRLAKNDSRVKLVRLDSNQGCYPARNAALRVAKGRYIAIQDSDDIS
                     LATRIETQLIPLLTGRALFTLTR"
     gene            complement(1418..2188)
                     /locus_tag="PKM34_04790"
     CDS             complement(1418..2188)
                     /locus_tag="PKM34_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ82937.1"
                     /translation="MGKPIIIDIKNVSKRYKLYRNKKDQALQALFPFLPRKHTDFYAI
                     KDFSLTVNKGDILGIMGRNGSGKSTLMKLVAGVIQPTKGTITVKGNLVPLLELGSGFH
                     REFTGYENIYFYTAVLGYPKKKVIEKTAEIIEFADIGEFIHQPLKNYSSGMKTRLAFS
                     VSVNIDPDILILDEVLSVGDAAFKTKSYDKIKEFFNQKKTILFVSHSPESIKALCTRA
                     ILIDKGKLVIDGDPAEVVKKYSSIYGGKKVSEKIMDLE"
     gene            complement(2201..3028)
                     /locus_tag="PKM34_04795"
     CDS             complement(2201..3028)
                     /locus_tag="PKM34_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ82938.1"
                     /translation="MKLIYLIKRFFIEGADFLKQIYLNKFIIWELTKRDFKTSYVSNI
                     LGLSWAILEPLAMMFVLWFVFTFIRTGQKTSVPFALFLLAGLIAYDLFNKALNSATRG
                     ISNYGFLINKVHFRSAIIPLVKISSELIMHGIILVIVSTILILSGIPVTIYWLQVFYY
                     LFATVFLLVGLTWLTSSVSLFFPDIKYIITIVMRVLFFFTPIFWEMKNIPENMVLIFR
                     LNPLYYIVKGYRDSFLYGIPFWSNWTDTAYFWGLAGAFFIIGVLVFKRLRPYFAEMV"
     gene            complement(3048..4004)
                     /locus_tag="PKM34_04800"
     CDS             complement(3048..4004)
                     /locus_tag="PKM34_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNQ82939.1"
                     /translation="MNKDSKIYIAGHNGMVGSALHRNLEAKGYRNFIFRTFDELDLTR
                     QDDVEAFFEAEEPEYVFDAAAKVGGIVANDTFRAQFIYENLMIQNNLIHSSYKNGVKK
                     FLFLGSSCIYPKLAPQPLKEEYLLTGSLEPTNEPYAIAKIAGIKMCENYYRQYRSNFI
                     SVMLTNLYGPNDNYDLQNSHVIPALLRKFHDAMISNAPSVTLWGTGQPLREFMHVDDM
                     ARACILVMEKLDAPKLYDDLGLTHINIGVGEDITILELGRLIAKITGYTGKILFDTSK
                     PDGTPRKIMDNSRLRKLGFKPRISLEEGLAQTYKMLSLSVQH"
     gene            complement(3997..5157)
                     /gene="gmd"
                     /locus_tag="PKM34_04805"
     CDS             complement(3997..5157)
                     /gene="gmd"
                     /locus_tag="PKM34_04805"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ82940.1"
                     /translation="MPKTALITGITGQDGAYLAEYLLKKGYIVHGIKRRSSLINTGRI
                     DHLYEDPHVENQKFILHYGDLTDSTNLIRIIQDVQPDEIYNLGAQSHVRVSFETPEYT
                     ANSDALGALRLLEAIRLLGLANKTRFYQASTSELYGLAQEIPQTEKTPFYPRSPYGAA
                     KLYAYWITVNYREAYNIFACNGILFNHESPIRGETFVTRKITRAVARIAMGLQDKLYL
                     GNLDARRDWGHAKDFVKAMHLMLQQDTPGDYVIATGKTTSVREFVKKAFAYIGAEIEF
                     RGEGVEEKGYLNKMVDGPLSMVDGRWKKIENRPSKIENRKSVVEIDPRYFRPTEVDIL
                     QGDASKAKAELGWEPELSLDDLIGDMMEADLELMRKEQFLREGGYEIMNYYE"
     gene            5267..>5819
                     /locus_tag="PKM34_04810"
     CDS             5267..>5819
                     /locus_tag="PKM34_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ82941.1"
                     /translation="MERSIPIVVVGYNRPKALKRLLNSLLKAEYPCDVELIISIDGGG
                     DEENKKIASSFSWPFGKKQLIFHEQNIGLRKHVLSCGDLVKDYDGVIVLEDDLYVSPG
                     FYEYTLKAFDFYKDDDKLAGISLYTHAYNETAQFPFRPLADGSDVFFLQYAPSLGQFW
                     SREGWRRFRDWYDTLVPGEPIPVN"
BASE COUNT         1322 a         1452 c         1524 g         1521 t
ORIGIN      
        1 atacgggtca gcgtgaacaa agccctgcct gtcagcaacg ggataagctg ggtttcgatc
       61 cgggtggcca gcgaaatgtc gtcgctgtcc tggatggcga tgtacctgcc ctttgccacc
      121 ctcagcgcgg cattgcgggc ggggtagcac ccctggttgc tgtcaagcct caccagcttc
      181 acccggctgt cgtttttcgc cagcctgtcc acgatagcta cggtgctgtc ggtactgccg
      241 tcgtcagcga cgatcatctc cagctcccgg taggtttgca tcaggatcga ccggatgctc
      301 ttttcgatcg tcttttctgc gttgtaagcc ggcatgatca cactcaccag ctccttccgg
      361 aacgggtagt cgtacagatc catctgccag cgcagtttcc tggccatctg cccctggtcg
      421 cgctccacgc cgatatcgct ctggccgacg tcggccacaa ccaggttggg cttaaccacg
      481 aagcactgcc cggggtatct ctgctgaatc gctctcagcg ggcccgaatc cagcgcacag
      541 ttcatcggtt cgatctccga aaggatctca tcgaataccg aacaatcgac cccaacggca
      601 aaagagccgt cggtcttaac gggataatag aaaggatgaa cgccaaaatc tttttcaccc
      661 actttgtcag gataattgac attgtaaggc acgctccagt cgtgctgcgt tgccccgagg
      721 tacaaaagct tccagtttcc gggtatggat ttcagcgatt ccccggtttt cttttcaaaa
      781 tctttatgaa aaaggacatc gtcatcaaaa acaagaatcc ttgcaaaccc cttactttta
      841 gcatcattca gcagatactt ccaggtaaga agatatcccc atgaaccggg gctggcaatc
      901 atcttcttct tcagctcctt ttccagggga tggcatccct cggccgccag cggcaggtcg
      961 taataagcgg caaaggcttc ggaattctct ttggaatacc cgttcccggc ctcgaatatc
     1021 tccgctttga tgcaatattt cctgagcttc atgatcatct ccacccgccg gtcgagccgc
     1081 cggcccaggt tgatcacata tatcctgtca aaaatatgat tgatgaccga taattcattt
     1141 tcatcaacgt catcattaat aataaagggc ttgtattcca gcgtcgggac aaagttgaac
     1201 aagccgcctg cgacatccgg atctgccaaa ccggtgttcc aggtacggtt acggacatct
     1261 cccctgatct tgcgggtgag gcggaaatcg tacatcagat cgaccatcct gacctggggc
     1321 agtcttcgct ctttcatcac cacgatgaaa cgctgtatca cccagaggat cgtccagggg
     1381 cggtatgttt tccgtaaaaa agccatcttt ctgtgcgtca ttcaaggtcc atgatcttct
     1441 cacttacttt tttgccgccg taaatggaac tgtatttctt gacaacttca gccggatcac
     1501 catcgatgac cagcttccct ttgtcgatca ggatggcgcg tgtgcataaa gctttaatcg
     1561 attcggggga atgggaaaca aacaggatgg tcttcttttg attgaagaac tcctttatct
     1621 tatcgtagct tttggttttg aaggccgcat cgccgaccga aagcacctcg tcgaggatca
     1681 ggatatcggg atcgatattc acggagacgg agaaggccag gcgtgttttc attccgctgc
     1741 tgtagttttt cagcggctga tggataaact cgccaatatc ggcgaactcg atgatctcgg
     1801 ccgtcttttc aatcactttt ttcttcgggt aacccaggac ggcggtataa aaatagatat
     1861 tctcgtagcc ggtgaattca cggtgaaatc ccgaacccag ctccagcagc gggaccaggt
     1921 tcccttttac cgttatcgtc cctttggttg gctggatcac cccggccacc agtttcatca
     1981 gggtcgattt gcccgatccg ttccggccca tgatccccag gatatcgcct ttattcaccg
     2041 taaggctgaa atccttaata gcataaaaat cggtatgttt tctcggaagg aaaggaaaca
     2101 gagcctggag tgcctggtct tttttattcc ggtacagctt gtatctcttg ctgacgtttt
     2161 taatatcgat tatgatcggt tttcccattt tcgatccccc ttataccatt tcagcaaaat
     2221 atggcctgag ccgtttgaat accagtactc ctattataaa aaatgcgcct gccaatcccc
     2281 agaaataagc agtatcggtc cagttcgacc agaaggggat cccgtaaagg aagctgtcgc
     2341 ggtaaccttt tacaatgtaa tacagcggat ttaacctgaa aatgaggacc atgttttcgg
     2401 gtatgttttt catttcccag aaaatgggcg taaagaaaaa caataccctc atgacaatgg
     2461 tgatgatgta tttaatatcc gggaagaaca gtgaaactga tgaagtaagc caggtaaggc
     2521 ccaccaggag gaatacagtg gcaaagagat aataaaatac ctggagccag taaatggtta
     2581 ccggtatccc gcttaatatc aggatggtag agactattac caggattatc ccatgcataa
     2641 tgagttccga agagatttta acgagcggaa tgatggccga cctgaaatgg actttattga
     2701 taaggaatcc ataattgctg atcccccggg tggctgagtt cagcgctttg ttgaacaggt
     2761 catacgcaat taaacccgcc agtagaaaga gggcaaatgg tacagaggtc ttttgcccgg
     2821 tgcgtatgaa agtgaataca aaccagagaa cgaacatcat cgccagcggt tcaagtatag
     2881 cccatgataa acccaggata ttgctgacgt agcttgtctt gaagtcgcgt ttggtcagct
     2941 cccagataat gaatttattg aggtagatct gttttagaaa atcggcccct tcaataaaaa
     3001 aacgctttat caggtaaatc agtttcactg cttaaataca gaccggctta atgttgaacg
     3061 gacaaagata gcattttata tgtttgcgcc aggccttctt caagtgagat gcggggtttg
     3121 aacccgagct tgcgcaaacg gctgttatcc atgatcttcc ggggtgtgcc atcgggcttt
     3181 gaagtgtcga aaaggatttt gcccgtatag ccggtgattt tagcgatcag gcggcccagt
     3241 tccaggatcg taatgtcttc tcccacgcca atattgatgt gggttaatcc aaggtcgtcg
     3301 taaagcttcg gtgcgtcgag cttctccatc accaggatgc aggcccgggc catgtcgtcg
     3361 acatgcatga actcccgcaa cggttggccg gtgccccaga gggtgacgga aggggcattg
     3421 ctgatcatgg catcgtggaa cttgcgcagc aaagcgggga tcacgtgcga attctgcagg
     3481 tcgtaattgt cgttcgggcc gtagaggttg gtcagcatca ccgagataaa gttggagcgg
     3541 tactgccggt agtaattctc gcacattttg atgccggcaa ttttggcgat ggcatacggc
     3601 tcattggtcg gctcaaggct cccggtcagc aggtattctt ccttcagcgg ttgcggggcc
     3661 agcttcgggt agatgcagga gctgccgagg aaaaggaact ttttcacccc gtttttgtaa
     3721 gatgaatgga tgaggttgtt ctggatcatc aggttttcgt agatgaactg ggcgcggaag
     3781 gtgtcgttgg ccacaatgcc gcccactttg gccgccgcgt cgaacacata ttcgggctct
     3841 tccgcctcga agaaggcctc cacatcgtcc tgccgcgtta agtcaagttc atcgaaggtc
     3901 cggaagatga agttcctgta acctttggct tcgagattgc gatgcagcgc cgagcccacc
     3961 atcccgttat gcccggcgat gtatattttc gaatctttat tcataatagt tcatgatttc
     4021 atatccccct tctcgcagga attgctcttt ccgcatcagt tccaggtcgg cctccatcat
     4081 atcaccgatc aggtcgtcga gcgaaagctc cggttcccag cccagttccg ctttggcttt
     4141 cgaggcgtcg ccctgcagaa tatcgacctc cgttggccta aaatagcgcg gatcgatttc
     4201 gacgacggat tttcgatttt cgattttcga tggtcgattt tcgatttttt tccatcgacc
     4261 atcgaccatc gacaatggac catcaaccat cttgttgagg tagcctttct cctcaacgcc
     4321 ttcccccctg aactcgatct ctgcgcctat gtaagcaaag gctttcttaa cgaactccct
     4381 gaccgacgtg gtcttgccgg tggcgatcac gtagtcgccg ggcgtgtcct gctgcagcat
     4441 caggtgcatg gctttaacga agtctttggc atggccccag tcgcgccggg cgtcgaggtt
     4501 gcccaggtag agcttatcct gcaagcccat ggcaatgcgg gccactgcgc gggtgatttt
     4561 gcgggtcacg aaggtctcgc cgcggatagg cgattcgtgg ttgaacagga tgccgttgca
     4621 ggcgaagatg ttgtaagctt cgcggtagtt gacggtgatc cagtaggcgt agagtttcgc
     4681 ggcgccgtaa ggcgagcggg ggtagaacgg cgtcttttcc gtttgcggta tctcctgcgc
     4741 caggccatag agttcgctgg tgctggcctg gtaaaaacgg gtcttgttgg ccagtcccag
     4801 caggcggatg gcttcgagca ggcggagggc gccgagcgcg tcggagttgg cggtatattc
     4861 gggtgtttca aagcttaccc tgacatgcga ctgggcgccg aggttgtaaa tctcgtcagg
     4921 ttgcacatcc tggatgatgc ggatcaggtt ggtcgagtcg gtcaggtcgc cgtagtgcag
     4981 gatgaatttc tggttctcca catgcggatc ttcgtaaaga tggtcgatcc tgccggtgtt
     5041 gatcagcgag ctgcgccgct tgatgccgtg gacgatgtag ccttttttca gcaggtactc
     5101 ggcgaggtag gcgccgtcct gtcctgtgat cccggtgatg agtgcggtct tgggcatagc
     5161 tggctgagtt tgcgttttga gtttccccgg aattaatccc ggggaaactc aaaaactgtg
     5221 ctaagttaaa catattattc tggactaaaa cctgtatttt tgatttatgg aacgatctat
     5281 acctattgta gtcgttggtt ataaccgccc gaaagcgttg aaacggctgc taaattccct
     5341 gctgaaagcg gaatatccgt gtgatgtgga gctgattatc agcattgatg gcggtggcga
     5401 tgaagaaaat aaaaaaattg ccagttcatt ctcctggcca tttggcaaaa aacaactcat
     5461 ctttcatgaa cagaatatcg ggttgcggaa gcatgtgctt tcttgcggcg acctggtcaa
     5521 agattacgac ggggtgattg ttctggagga tgatctgtat gtgtcgcccg ggttttatga
     5581 atatacgctg aaggcttttg atttttataa ggatgatgat aaactagcgg gcatttcgct
     5641 ttatacgcat gcttacaacg aaacggccca gttcccgttc cggccgctgg cggatggttc
     5701 ggatgtattc ttcctgcagt acgcgccgtc gttgggacag ttctggagca gggaagggtg
     5761 gaggcgcttc cgggattggt acgatacgct ggtccccggc gagccgatcc cggtgaacc
//
LOCUS       DAOQXS010000125         5816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14080_length_5816_cov_3.609443, whole genome shotgun sequence.
ACCESSION   DAOQXS010000125 DAOQXS010000000
VERSION     DAOQXS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14080_length_5816_cov_3.609443"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2914)
                     /locus_tag="PKM34_04815"
     CDS             complement(<1..2914)
                     /locus_tag="PKM34_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNQ82942.1"
                     /translation="MNFKSLFFIVLVFSGTSMGLRAQQGIAINTTGASPDGSAILDVS
                     STSKGILIPRLDNGQMSGMSNPADGLLVYNTTANCFFYFESTGPGTGAWRQLIDNANN
                     NTLYDIDGDSYLTFKYDGGHDDTLYMLFDETEKYRITAKAIEMLNNSRSVMIGEGAGQ
                     YMDLTGDKHNIYIGYHAGNTNQYGGANTAVGHYALSENTVHNNTAIGSSALKFNTTGS
                     YNTAIGAGSQMNGVSGSSNTSVGYQSMIYSKGVENVAIGKQAMFYDTTGAYNVAVGNN
                     ALMYSRSSSSNTAIGHGALNMSSHGNGNVAVGYESMYLDSAGSNNTSIGQRSLYNNKN
                     GSYNLALGAYAAYNSRSSTGNMAIGAYSLYYSQNTTNNAAIGYGAMEQLTEGTNNIAI
                     GSNALRYSENVSENTVIGMNAMMFNKTGSRNTVIGYYAMIFDSIGSGNCILGHNAAYA
                     NKKGNDNVAIGQAALMNNTIRGDLVAIGDSALYSNGMGIIESQSYTAACNTAVGSNAM
                     MNNSSGYNNTALGFKCLYNNTQGDGNTAVGYKGMYTNSSGAWNTSLGYMAMHNNTGGI
                     NNTAAGYTAMMNNTMGIGNTALGQAALKTNTEGYYNVALGNAAMLSNTLGTSNIGIGN
                     EALKSNTTRGHLIAIGDSALYRNGIGATEIQHATKNIAVGSKSLYYNSTGRSNTSIGF
                     EAMTNNNGGSENVAVGTQALYNNTSGAGNTAAGVSALLSNSGGYENTAFGYHALEDNN
                     GNYNSGFGSFALMNNASTGFNNTAVGAYALGLVTTGDNNTAIGYYTASGANFANSTFI
                     GASIDITTARTNISVLGYGVTNGECTGNNQVLLGNTSISQIRAMVGSITTYSDARIKS
                     NVEENVPGLDFVCKLRPVTYNKDPEKLHQIWGTPAEQVAKIDFTDTRQQRYIGFLAQE
                     VEQAALDCGFEFPGIDVPANDHEAYSLRYGDFIMPLVKAVQEQQAQIEELKAANAML"
     gene            complement(2919..3071)
                     /locus_tag="PKM34_04820"
     CDS             complement(2919..3071)
                     /locus_tag="PKM34_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82943.1"
                     /translation="MAGSKKKPATENLNEMLKRIRSENKALEKLIRALEIRTAKKGEK
                     IKTNES"
     gene            3397..3744
                     /locus_tag="PKM34_04825"
     CDS             3397..3744
                     /locus_tag="PKM34_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ82944.1"
                     /translation="MYDLEERTEKLAIEVRNFIKILPKTISNVEDSKQLIRSSGSIGA
                     NYIEANESLSRKDFVMRIKISRKEAKESIYWLNIIQESNNLDEQTKIRCESLIKETGE
                     IRNMLSAILKKFV"
     gene            complement(3846..5693)
                     /locus_tag="PKM34_04830"
     CDS             complement(3846..5693)
                     /locus_tag="PKM34_04830"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ82945.1"
                     /translation="MNRIHRQSLFIKSFICCLLLIVSAGLRAVPEDGKTLTDLITRAE
                     SLVESDPGEAKSLLRIAAARSDPKRFPKQTSRINFLLGEASFYLDDPDSVIAYYLKAV
                     EIDVASGNDRTAEHINILGNLGYMYDLIDQKQIAIDFYQKALNIAREINQKDEIAANL
                     ANIAQLKTLQGFYEEAMLYMEEALAFDKESGDESIIAIDLNTIGRIYESMGMYAQAVS
                     YLEQALEIDLRLKNEDKVAIRYNSLGLVYKGWGKYNMALEYFGKALEIDQKLGREEKV
                     ALRQANIGSTLIESGEVDRAIAYLEESLDYFKTNDRPSYTATTLNDLGRGYFMKKDYA
                     KAEKLFLESANICRQYGFVRFRMNSLDYLSRLYRESGQYQKAYLSISEFNVLNDSIFN
                     AESQKKIAEFNAKYELDKEQQENELLRRDREISRKRHTVAILAFSLAGLVLALFLLAV
                     LVRLKTIQNRRLRAEKENERLRMDLEQKNKELTYNAMCIIKNNETVAKIAETLEDAIQ
                     AGQDERGINNLVRKLQNMEREKNWTEFEVRFTKVHEDFYRKLNERFPDLTPNEKKLCA
                     FLKLNMSTKDIAAITHQSVHSINVARTRMRKKLGIDGTDENLIGFLNSL"
BASE COUNT         1417 a         1375 c         1296 g         1728 t
ORIGIN      
        1 tgagcatggc attggccgct ttcagttctt cgatctgggc ctgctgctcc tgcacggctt
       61 tcaccagcgg catgatgaaa tcgccgtagc gcagggaata agcttcatgg tcatttgccg
      121 gaacatcgat gccgggaaat tcgaagccgc agtcaagggc agcctgttcc acttcctggg
      181 ccaggaatcc gatatagcgc tgctgccggg tatccgtaaa atcaatcttc gcgacttgtt
      241 cggccggcgt accccagatc tggtgaagtt tctccgggtc tttgttgtaa gtgaccggcc
      301 gcagcttaca gacaaaatcg aggcccggaa cattctcttc cacgtttgat tttatccgtg
      361 cgtcgctgta agtggtgata ctgccaacca tcgccctgat ctggcttatc gaggtgtttc
      421 caagcaaaac ctggttgttg ccggtgcatt caccgttagt gacgccgtat cccagcaccg
      481 aaatattggt ccgtgctgta gttatatcaa tagaggcacc gataaatgtg ctgtttgcaa
      541 agtttgcacc ggaagcggta taatatccga tggccgtatt gttatcgccg gtagtgacca
      601 gtcccagtgc gtaagctcca acggcagtgt tgttaaatcc ggtcgatgcg ttgttcatca
      661 gggcaaagga cccgaatccg ctgttataat tcccattgtt gtcttccagg gcatggtagc
      721 caaaggcggt attttcgtac ccgccagagt tgctgagcaa ggcgctaaca cctgctgccg
      781 tattccctgc ccccgacgta ttattataca atgcctgggt tcctaccgcc acattctccg
      841 atccgccatt gttatttgtc atggcttcaa agccaataga agtattgctc ctgcctgtcg
      901 aattgtaata taaacttttt gatccgaccg ctatattttt tgtggcatgc tggatttcgg
      961 ttgcgccgat cccgttccgg taaagcgccg agtctccgat ggcgatcagg tggccgcggg
     1021 tggtgttact tttcaaggct tcattcccga taccgatatt ggaggttccg agcgtgttgc
     1081 ttaacatcgc agcattgccg agggctacat tgtaataccc ttccgtgttg gttttaagcg
     1141 ctgcttgccc cagggccgta tttccgattc ccatggtgtt attcatcatg gccgtgtaac
     1201 ctgcggctgt attgttgatc cccccggtat tattgtgcat ggccatgtag cccaaagatg
     1261 tattccaggc cccgctgctg ttggtgtaca ttcccttgta gcccacggca gtattgccat
     1321 cgccctgtgt attgttatag aggcatttaa accctaaggc agtattgtta taaccggatg
     1381 agttgttcat catggcattg gatccgaccg ccgtgttaca ggctgctgta tacgattgtg
     1441 attcaataat gcccattccg ttgctataaa gcgctgaatc accgatagct acaagatcgc
     1501 ctcttatggt attattcatc aaggcggcct ggccgatggc cacattatca ttgccttttt
     1561 tattggcata agccgcgtta tgtcccagga tgcagttgcc ggacccgatg gagtcaaaga
     1621 tcatggcata atatccgatg accgtatttc ttgatccggt cttattaaac atcatggcat
     1681 tcatcccgat caccgtattt tcactgacat tttccgaata gcgcaatgca tttgaaccga
     1741 tcgctatatt atttgttcct tcggtcaatt gttccatagc cccgtatcca atagcagcat
     1801 tgttggtcgt attctggcta taatataatg aataggcgcc gatagccata ttccctgtgc
     1861 tactcctgct gttataagca gcataggctc ccagggcaag attatagctt ccgttcttat
     1921 tattataaag tgatcgctgt cctatcgatg tgttgttcga accggcggaa tccaggtaca
     1981 tagactcgta cccgaccgcc acattcccat tgccatgact gctcatattg agcgccccat
     2041 ggccgatagc cgtattggaa ctgctgcttc gcgaatacat caacgcatta tttcccacag
     2101 cgacattata ggctccggtg gtatcataga acatagcttg ctttccgatc gctacgttct
     2161 caactccttt cgaataaatc atcgattgat aacccactga ggtgttggaa gaacccgata
     2221 ctccattcat ttgtgaacca gcgccgatgg cggtattata actgcccgtc gtgttaaatt
     2281 tcagggccga gctgccaatt gcagtgttat tgtgcaccgt attctccgat aatgcataat
     2341 ggccgacagc cgtgttggct ccaccgtatt gattcgtatt tcctgcatga tacccaatat
     2401 aaatattgtg cttgtcgcct gtcaggtcca tgtattgccc ggccccttcc ccgatcatca
     2461 ccgaccggct gttgttcagc atttcgattg cttttgcagt gatccggtat ttttccgttt
     2521 catcgaaaag catatacaag gtatcatcat gccctccgtc gtacttgaat gtcaggtagc
     2581 tgtcgccgtc aatgtcatat aaggtattgt tattggcatt gtcgatgagc tggcgccagg
     2641 ccccggtgcc aggacccgtt gattcaaagt agaaaaagca gttagcagta gtattataaa
     2701 ccagaaggcc gtcggcagga ttcgacatgc cggacatctg cccgttatca agccgtggga
     2761 tcaggatccc tttggaagtg ctgctcacat ccagtatggc tgagccatcg ggcgatgcgc
     2821 ctgtagtgtt gatagcgata ccctgctgag ccctgaggcc catgctggtt cctgaaaaga
     2881 ccaggacaat gaagaataac gatttgaaat tcatattttt atgattcatt ggtttttatt
     2941 ttctctccct ttttagccgt cctgatctct aaagcccgga tcagtttttc caatgcttta
     3001 ttctctgacc ggatgcgttt cagcatttca ttcaggtttt cggtcgccgg ttttttcttg
     3061 gatcctgcca taataattca gagcgtaaat gtaaaaggca ggacatggaa aatgcaaatt
     3121 ttaacgtgat actttattga tacagacggg gaaattccaa attccaagca ccaagctcca
     3181 aacaaattcc aaatcccagg caccaaaatc caaacaaaac tcaaaactca aatttaaaac
     3241 aaaccaaaaa actgatccct cttccaggct cctaacgtat tgttatgtaa atatctacgg
     3301 gatccaattc gtttcttata aaattttttt tctgattacc gggtgtattt tcaaaaaatc
     3361 ctgataaata gatatgaact tataaccggc attcccatgt atgatctgga agaaagaaca
     3421 gagaaactgg cgatagaagt tagaaatttt atcaagatct tgccaaagac aatttcaaat
     3481 gtcgaggata gtaaacaatt gattcggtcc tccggatcaa taggggcaaa ctatatcgaa
     3541 gccaatgaat cccttagtcg caaagacttt gtaatgagaa ttaaaatcag cagaaaagaa
     3601 gccaaggaat cgatttactg gttaaacatt attcaagagt caaataatct cgatgagcag
     3661 actaagatca ggtgtgaatc gctaataaaa gaaaccggtg aaatcaggaa tatgctttca
     3721 gcaatcttga aaaaatttgt ttgaagcaat atttgaattt gatatttgtt ttttgaattt
     3781 gtttgggttt tggaatttgg tgcttggaat ttgtttggag cttggtgctt ggaattttgg
     3841 aattttcaaa ggctgttcaa aaacccgata agattctcgt ctgtgccatc aatccccagc
     3901 tttttccgca tccgggtgcg ggctacgttg atactgtgaa cactctgatg ggtaatggcg
     3961 gcaatgtctt tggtcgacat atttagttta aggaaggcac aaagcttctt ttcattgggg
     4021 gtaaggtcgg ggaagcgctc attcagtttc cggtagaaat cttcgtggac ctttgtgaaa
     4081 cgtacctcaa actcggtcca gtttttctcc ctttccatgt tttgaagttt acggaccaga
     4141 ttgttaatac ctctttcatc ctgtcctgcc tggattgcat cttccagcgt ttcggcaatt
     4201 tttgccacag tctcattatt cttgatgatg cacatagcgt tgtaggtcag ctctttgttc
     4261 ttctgctcca gatccattct cagccgttca ttttctttct cggccctgag cctgcggttt
     4321 tgaatagtct tcagccttac caggaccgcg aggagaaaaa gcgccaggac aagcccggcc
     4381 agcgaaaaag caaggatagc gacggtatgc cttttccggc tgatctcccg gtcacggcgc
     4441 aggagttcat tttcctgctg ttccttgtcg agctcgtatt tggcgttgaa ttcagcaatc
     4501 tttttctggc tttcggcatt gaaaatcgag tcgttcagca cgttgaattc cgaaatggag
     4561 aggtaagctt tctggtattg acccgattcg cggtaaagcc tggagagata atcgaggctg
     4621 ttcatccgga accggacgaa gccgtattgc cggcagatat tggcactttc cagaaagagt
     4681 ttctctgctt tagcgtagtc ctttttcatg aaataccctc tgcccaggtc attcagggta
     4741 gtagcggtat aagaaggacg atcatttgtt ttaaaataat caagactttc ttccaggtag
     4801 gcgatagccc ggtcaacctc tccgctctcg atcaaagtac tgccgatatt agcctgccgc
     4861 agggccactt tttcctcccg ccccagtttc tgatcaattt ccagcgcttt tccgaaatac
     4921 tccagcgcca tgttatattt cccccatcct ttataaacca gtcccaggct gttataacgt
     4981 atggccactt tatcttcgtt tttaagtcgc agatcgattt ccagcgcttg ttcgagatag
     5041 ctgacagcct gtgcatacat gcccatcgat tcatagattc tcccgatcgt gttgagatca
     5101 atggcaatga tggattcgtc gcccgactct ttatcgaatg ccaatgcttc ttccatatag
     5161 agcatggctt cttcataaaa tccctggagg gtttttaact gggcaatatt ggccaggttg
     5221 gccgcgattt catctttctg gttgatttcc cgtgctatat tcagcgcttt ttgatagaaa
     5281 tcgatggcta tttgcttctg atcgatgagg tcgtacatgt agcccaggtt acccaggata
     5341 ttgatatgtt ccgcagtccg gtcattaccg gatgccacat cgatttcaac tgctttcagg
     5401 tagtacgcga tgactgaatc ggggtcatcc aggtaaaaac tggcttcacc caggaggaag
     5461 tttattctgg atgtttgttt cggaaaacgt ttgggatccg atctagcagc ggcaatccga
     5521 agcagggatt tcgcttcacc ggggtcgctt tccaccaggc tttctgcccg ggtgatcagg
     5581 tcagtcagtg tttttccatc ttcaggaaca gctcgtaagc ctgcggaaac aattaataat
     5641 aaacaacaga taaatgactt aataaacaaa gattgacggt gtatcctatt catagccaga
     5701 atcgatatcg acttctaaaa taatcaaatt tgatcatctg ttcatcaata aaataattcg
     5761 cactaagtac atgacaaaaa tttacaaata tctttcaatt tatctatatc attgga
//
LOCUS       DAOQXS010000126         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14106_length_5808_cov_3.641404, whole genome shotgun sequence.
ACCESSION   DAOQXS010000126 DAOQXS010000000
VERSION     DAOQXS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14106_length_5808_cov_3.641404"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PKM34_04835"
     CDS             complement(<1..1758)
                     /locus_tag="PKM34_04835"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82946.1"
                     /translation="MKNILLLFSSIIFLSGFFNSEKVNSQICCPDFILKDAVEICAPH
                     MSCSSDQPGIGFPVAACKNTVHTYTVYPNDLSSFTYTWTVTGGTPSSTNGNPVNILWG
                     NGASGYIKVVITSNNPNFICADSIMQQICLIDGPQAAFTVNYDTVCTGVAISLTNTSL
                     GGSSFTWNMGDGTIYTSPTPPNHTYQSPGTYTIILTVKDMGQGQYVGGPNGENRIPCG
                     CADTALAVVVVLPGIGPEIETDCCYGTVCPGDTSEFCTPMVCGSYNWTVTGGTIISGA
                     GTSCIQVEWDNTYSVPTTVSLQSCPSSSCPGSTTIDVPVLYPNLPISGPNNLCLGASG
                     TFSLPHLPGTFYKWTVSGGPYIFNEKNRNVSLVNISFNSFGTYWVKCEYNNPLAGCDG
                     VDSLLVTVTPKFDIVGPDKICEGDTVFYFTNSLVGGPPANWTFSPAGPVVYTGQGTPN
                     PSIIASPGNYTIIATSLTPPVNCNLVATKNIQVVAKPLLGNINGPTMACPNTNYTYGI
                     TSNTSGSSFIWSVGPGSGAIFSQMGDDNDSVIVQFSGSGPWTLNVYQEIDLGNNDFCK
                     SLTKTLVVNPFPAPNITGAH"
     gene            complement(1971..2384)
                     /locus_tag="PKM34_04840"
     CDS             complement(1971..2384)
                     /locus_tag="PKM34_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNQ82947.1"
                     /translation="MTDDLNQRISKAKQLFDGPYNCSQSVAVAFADILQKDEQEILKL
                     MSGFGFGMGGERSVCGAVSGGIFIISSTADDPAIREEIYDKVFYLVSQFKKLNQGNLH
                     CLDLVGKEPTDEQFRTTCPVLLSQVITLVRKIIKD"
     gene            complement(2444..3778)
                     /locus_tag="PKM34_04845"
     CDS             complement(2444..3778)
                     /locus_tag="PKM34_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ82948.1"
                     /translation="MNKKCLKRWTILLLTSGWVLQGFGQEILTLKDAIRIGLENNYSI
                     IIQKNEAEIAANNNTLGNAGFLPTVNLTGTQNNTVTNTHQETFSSGAKDITGATNRTL
                     NAGIQLTWTLFDGFSMFAGKNMLKIMEEMGETQTRITVENTLSSIIQTYYGIIQQQKL
                     IAVLEDAAALSLERKKIMEARISLGSGSELQFLQATVDLNADSINLIRERSVLQKSMA
                     DLNRLLARSPEITFIPADSIKLQTRLNYDSLLAKTRGQNRELQMARNNLSLSNFALKD
                     IRSQRYPRLNFNAGYSYSQLRSETGFLQYNRSLGPSFGVSLSYSLFNGFNVNREIQNA
                     RLDLNTMENYLLQTDLSLHTSLYQQYNDYLTNLRIVDMELINREVARENVEVAFAKYR
                     LGTINDIELRETQKKYIDAQYQLLLSQFQAKNAEIELLRISGELFKVLSVGF"
     gene            complement(3771..>5808)
                     /locus_tag="PKM34_04850"
     CDS             complement(3771..>5808)
                     /locus_tag="PKM34_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ82949.1"
                     /translation="IFIFLRTWRTTLIPVIAIPISLVSGFFIMYIAGFSINILTLLGI
                     VLATGLVVDDAIVVLENIYHKVESGMNPVEAGHKGSKEIYFAIISTTITLAAVFLPVI
                     FLQGLTGRLFREFGMVVAGTILVSALVSLTLTPMMSSRMLRKTEKHSRVFNRTERWID
                     GLISGYNKRLTSFLRYRWLAILIMVLSIGMILGIGSLVPSELAPMEDKSRLSIVSTAP
                     EGTSFEVMDEYMLSLLEVVDTIPEKTNFLAITAPGFGSSASTNTGFFRVILTDPSERE
                     RSQQEIADELSQITRRYNFARSFVTQEQTIGGGRGGGLPVQFVILAPNFDKLRAVIPR
                     FMEEAQKSPLFQVTDLNLKFNKPEVSIDIDRNRARALGVNVRDVAENLQLYFSGQRYG
                     YFILNNKQYQVIGQADRLNRDEPLDLSSVYVRNNRNELIQLDNMVTLSENSNPPQLYR
                     YNRYVSATVSAQPAKGITLGQGIDEMRRIAGETLDDSFSTELAGVSKEYEDSSSSLLF
                     AFLLALVLIYLILAAQFESYIDPLIIMFTVPLALAGAVLSLWLFGQTINIFSQIGVIV
                     LIGIVTKNGILIVEFANQRKAAGLSIREAVIDAATRRLRPILMTSLATALGALPIAMA
                     LGASSQSRIPMGITIIGGLIFSLILTLFVIPALYTFLSRKIKKTEYEPYAGENE"
BASE COUNT         1395 a         1401 c         1557 g         1455 t
ORIGIN      
        1 atgtgccccc gtgatatttg gcgccgggaa aggattcacc accagcgttt tggtcagcga
       61 tttgcagaag tcgttgttgc cgaggtcaat ttcctggtaa acattcagtg tccaggggcc
      121 gcttccgcta aactggacga tgaccgaatc gttgtcgtcg cccatctggg agaatatggc
      181 gcctgaaccg ggacccacgg accagataaa ggaactgccg cttgtattgg aggttatgcc
      241 gtatgtgtag ttggtattcg ggcaggccat tgttggtcca ttgatatttc cgagaagagg
      301 tttggctaca acctggatat ttttagtggc gaccaggttg cagttaaccg gtggagtgag
      361 ggatgtcgca atgatcgtat agtttcccgg ggaagcgatg atggaaggat taggcgttcc
      421 ctggcctgta tagacaacgg ggccggcagg ggagaaggtc cagttggccg gtggtccgcc
      481 gacaagggaa ttcgtaaagt agaagacagt gtcgccttcg cagatcttgt ctgggccgac
      541 aatgtcgaac ttcggtgtaa cggtgacaag caaggagtcc accccgtcgc aaccggcaag
      601 cggattgttg tattcgcact tcacccaata ggttccaaaa gaattgaagg agatgttgac
      661 gagtgagaca ttcctgtttt tttcgttgaa aatataaggc ccaccggaaa cggtccattt
      721 gtaaaatgtt ccgggaagat gaggaaggga aaatgtgcct gaggccccga ggcacagatt
      781 gttcggaccg gagatcggca gattggggta cagcaccggc acatcgatcg ttgtcgatcc
      841 cgggcaggag gaggagggac aactctgcaa agaaactgta gtcggtacgg aataggtgtt
      901 gtcccattca acctggatgc aggaggtccc ggcgccggaa atgattgtgc caccggtaac
      961 ggtccagtta taacttccgc agaccatcgg cgtgcagaat tccgatgtat cccccgggca
     1021 gacggtgccg taacagcaat ccgtctcgat ttccgggccg ataccgggca gcacgactac
     1081 caccgccaaa gctgtgtcgg cacagccgca ggggatccgg ttttctccgt tcgggccgcc
     1141 gacgtactgg ccctggccca tgtctttcac cgtcaggatg atcgtataag ttccgggtga
     1201 ctggtaggtg tgatttggcg gtgtcgggga ggtatagatt gtgccatcgc ccatgttcca
     1261 ggtgaaggaa ctgccgccga gcgacgtatt ggtcagtgag atggccactc cggtgcagac
     1321 agtatcataa ttcacggtga aagcggcctg cggaccatcg atgaggcaga tctgctgcat
     1381 gatcgaatcg gcgcagatga aattgggatt gttactggtg atgaccacct taatgtatcc
     1441 cgaggcgccg tttccccaga gaatgttaac gggattgcca ttggtgctgg aaggtgtgcc
     1501 gcctgttact gtccaggtat aggtgaatga agagagatcg ttgggataaa ccgtatatgt
     1561 gtgtactgtg tttttacagg ctgccaccgg gaagccgatt ccgggctggt cgctgctaca
     1621 gctcatatga ggcgcgcaga tctcgacggc atccttaagg ataaaatcgg gacaacaaat
     1681 ctgtgaattt accttttcgg aattaaaaaa tcctgaaagg aaaatgatgc tgctaaaaag
     1741 gagtagaatg tttttcataa ccggagggaa tttaataagt gtgcaaaagt acctcatcat
     1801 catccgtata atacagatta tgaaaacatt aacaaaacat taacagaata ttaggagaaa
     1861 gttaacaaaa gggtggtaag aggtgtaagg ttggtaagga caatggacaa tggacaatga
     1921 acaatggaca atggacaatg aacaatggac aatgtaatgg cattacgtcg ctaatctttg
     1981 ataattttcc ggacgagggt gatgacctgg gagagcagga ccgggcaggt ggtcctgaac
     2041 tgctcatcgg ttggttcttt gcccaccagg tcaaggcaat ggagatttcc ctggttgagt
     2101 ttcttaaact ggctgaccag gtaaaagact ttatcatata tctcctcccg aattgccgga
     2161 tcatcggcgg tggatgaaat gatgaagatc ccaccggata ctgccccgca gaccgatcgt
     2221 tcgccgccca tcccaaatcc gaaaccgctc atcagtttta atatctcttg ttcgtctttt
     2281 tgtaaaatat cggcaaaggc aacagctacc gattgagaac agttataggg accgtcaaac
     2341 agctgttttg cttttgaaat cctttgattt aaatcatctg tcatagtatc gttataaggt
     2401 taaaaagtaa tttccagctt actcctgact ccaaactcag cactcaaaac ccaacactca
     2461 acactttaaa tagttctccg cttatccgga gcagttcgat ttccgcgttc ttagcctgga
     2521 attgggataa caggagctga tattgcgcat cgatatattt cttctgtgtt tcccgcagtt
     2581 cgatatcgtt gatggtcccc agccggtact ttgcgaaagc cacttcaaca ttttccctgg
     2641 ccacttccct gttgatcagt tccatatcga caatcctcag atttgtcaaa tagtcgttat
     2701 attgctggta aagtgaagta tgaagactga gatccgtctg tagcaggtaa ttttccatcg
     2761 tattcagatc caggcgggca ttctgaattt cccggttaac attaaaaccg ttgaacaatg
     2821 aatacgacaa cgacaccccg aaagagggcc cgaggctgcg gttgtactgg agaaatcccg
     2881 tttccgaccg gagctggctg taactgtatc cggcattgaa attcagcctg gggtatcgct
     2941 gcgaacggat atctttcagg gcaaaattgc ttagtgacag attattccgg gccatctgca
     3001 gttccctgtt ctgtccgcgg gttttggcaa gcaggctgtc gtaattcagc cgggtttgca
     3061 gtttaatgct gtctgccgga atgaaagtga tctccgggct tcttgccagc aaccggttca
     3121 ggtcggccat ggatttttgc aggaccgatc tttccctgat caggttgata ctgtcggcgt
     3181 tcaaatccac ggtggcctgc aggaattgca gttcggaacc tgatcccagg ctgatccggg
     3241 cctccatgat ctttttcctt tccagcgata aagccgcggc atcttcaagt acggcgatca
     3301 gcttctgctg ctggataatg ccgtaatagg tctgaatgat ggatgatagt gtgttttcaa
     3361 ccgtgatcct ggtttgtgtc tctcccattt cctccatgat cttcagcata tttttacctg
     3421 caaacatgct gaagccgtcg aataaggtcc aggtaagctg gatcccggca ttgagggtgc
     3481 gattcgtagc gccggtaata tcctttgcac cgctggaaaa ggtctcctgg tgcgtgttgg
     3541 tgaccgtgtt gttctgtgtc cctgtcaggt tcacagttgg cagaaacccg gcatttccca
     3601 gtgtattatt gttggctgca atttccgcct catttttttg gataatgatg gaataattgt
     3661 tttccagccc gatccgtata gcatccttca gggtcaggat ttcctggcca aagccctgga
     3721 ggacccatcc cgatgtcaat aacaggatag tccatctttt caaacatttt ttattcattt
     3781 tcaccggcat aaggctcata ttcggttttt ttgatttttc ttgaaagaaa agtatacaaa
     3841 gccggtataa caaataaagt caggatcagc gaaaagataa ggccaccgat gatggttatt
     3901 cccatcggga tccggctttg ggatgaggca cccagggcca tggcgatggg aagggcgccg
     3961 agcgctgtag ccaggctcgt catcaggatc gggcggagcc gtcgtgtcgc cgcatcgatc
     4021 actgcttccc ggatactcag cccggccgct ttccgctggt tggcgaattc tacgatcagg
     4081 ataccgttct tggtgacgat cccgatcagt acgatcaccc cgatctggct gaagatgttg
     4141 atggtctggc cgaacagcca caatgaaagc actgctcctg cgagcgccag cggaaccgtg
     4201 aacatgatga tcagcgggtc gatatagctt tcaaactggg ctgccaggat caggtagatg
     4261 agcacaaggg ccagcaggaa agcaaataaa aggctgctcg aactgtcttc gtattctttc
     4321 gatacacctg caagctctgt cgagaaactg tcatcaaggg tttcgccggc aatcctgcgc
     4381 atctcatcaa ttccctgccc gagggtgatc ccttttgcgg gttgcgccga aacggtcgcc
     4441 gagacatacc ggttatagcg gtaaagctgc ggcggattgc tgttttcgct caatgtgacc
     4501 atattatcga gctggataag ctcgttgcgg ttatttctta catagaccga actcaggtcg
     4561 agcggttcat cccggtttag acggtcggcc tgcccgatca cctggtattg tttgttgttg
     4621 aggatgaaat agccgtatcg ctgtccgctg aaataaagct gcaggttctc cgccacatcc
     4681 ctcacattca cccccagtgc ccgggcccgg ttccggtcga tatcgatact cacctccggc
     4741 ttgttgaatt tcaggttgag gtcagttacc tggaataacg ggcttttctg ggcctcttcc
     4801 atgaatctcg ggataacggc cctgagcttg tcaaagtttg gcgccaggat gacaaactga
     4861 acaggcagcc cgccgcccct tccaccgccg atcgtctgct cctgggtaac aaagctccgg
     4921 gcaaagttgt acctgcgggt gatctggctg agctcgtcgg ctatctcctg ctggctcctc
     4981 tccctttcgg aaggatcggt caggataacc ctgaagaacc cggtattggt cgaggcactg
     5041 gaaccaaatc cgggcgcggt aatagccagg aaattggtct tttcggggat ggtgtccacc
     5101 acttccagca aactcagcat atattcgtcc atgacttcga aggatgtccc ctccggtgct
     5161 gtggatacaa tactaagtcg acttttgtct tccatgggtg ccagttccga cggaacgagg
     5221 gaaccgatcc ccaggatcat tccgatggaa aggaccatga tcaggatggc cagccatcgg
     5281 tagcgaagga atgaagtcag acgtttgtta tagccgctga tcaggccgtc gatccagcgt
     5341 tccgtccggt tgaaaacccg gctgtgtttt tccgttttcc ggagcatccg ggaactcatc
     5401 atcggggtca gggtcaatga aactaaggct gaaacgagga tagtccctgc caccaccata
     5461 ccgaactcac ggaacaatcg tccggtaagc ccctggagga agatcacggg aagaaaaacc
     5521 gccgccagtg tgatcgttgt ggaaatgatg gcgaaataga tctctttgga gcccttgtgc
     5581 ccagcttcta ccgggttcat cccgctttcc accttatggt atatgttttc cagcaccacg
     5641 atggcatcat ccaccaccag gcctgtagcc aggacaatcc cgagaagggt cagaatgttg
     5701 atggagaaac ccgcgatata catgataaaa aatccgctga ccagggagat cggtatggcg
     5761 atgaccggta tgagggtcgt acgccaggtg cggagaaaga taaagatg
//
LOCUS       DAOQXS010000127         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14111_length_5805_cov_3.848696, whole genome shotgun sequence.
ACCESSION   DAOQXS010000127 DAOQXS010000000
VERSION     DAOQXS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14111_length_5805_cov_3.848696"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PKM34_04855"
     CDS             complement(<1..1547)
                     /locus_tag="PKM34_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82950.1"
                     /translation="MKTLKLNFHFPAFLRSYCLTVLPSYRHFFLPSYRLIVLSLLLSN
                     GIYAQNEANIWYFPQLCGLDFNSGEPVVLDDSQVLNGHIGSTICDSSGNYLFSADNEN
                     IFTSYGIMPNGTEMLRPGLSGAAIVEWPGRAGIYYYITAMESGWPQYGLYYSIIDINQ
                     GLGGVVLDKNIEIDAGWDVSDRVAIVRKENSDAVWVITRKYIEDAYAAFLVDHNGLNP
                     NPVISVMPDRENHWGYIKISSDKRYLVSSYHQNIEICGFNALNGEVSCLYTMKGPVEE
                     LNEAAEGLEFSPDSKYLYISYFTNFDTTVLYQYDMQYVTDSAEFINSALKIYHNANLH
                     SMQLARDGKIYCGPKEEPGPNADQTYLSVIHKPWIRGTGCMYEENAVSIYPNECVYSF
                     INILVDYLFRFEWIGETCQGYPIQFKPNFIPTPQTIQWNFDDGPGSSSWQLSPTYTFK
                     NPGVHEVKVDVWYPSGRYEHTSREIEISPSPLPLLGNDTLICNGSTITLNANCDADFF
                     SWSTGQFG"
     gene            complement(1992..4502)
                     /locus_tag="PKM34_04860"
     CDS             complement(1992..4502)
                     /locus_tag="PKM34_04860"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82951.1"
                     /translation="MKYLALLFLLVATLVSAVIAIHLIINKPAPETGKIPNDWFFLQR
                     SFPYPEINYDARAMAWKQSQTLRKKSDERGEGWLLKGPLNIGGRIAAMAMITYGTQSV
                     YAGAASGGIFKSSDLGQSWRPIFDEAMSLSIGDIAIAPSDTSVIWVGTGEANAGGGSM
                     AYDGNGIYLSEDAGLSWNPVGLNNSGSIGRLVVHPDDPQTCYVAAMGRLFSDNPERGV
                     FKTSDGGGTWDQVLYINDSVGAIDIVMHPAKPDTLYAAMWERVRRPHRRDYGGPGSGI
                     YRSYDGGDTWTLLTNGLPAASAQVGRIGIDISASDPNILYAIYADNIGYFDGLYKTVN
                     GGNSWFRTSDEELADCYASYGWWFGRIAVDPQNPDVAYVIGFDLYKTIDGGNNWSYIS
                     SDVHVDQHDIVIHPDNTDLLVLGNDGGVYFSYNGGYNWDFCDNLPITQFYTCEADEQY
                     PFRLYGGTQDNGTNRTYTGALDNWMMIYGGDGFHVLVDPVNNSYVYAEYQYGNLARST
                     NGGSSFSSAMSGISGSDRKNWNTPVVFDPSDPQILYYGANRLYRSTNRAVSWQAVSPD
                     LTGGSGSGSVVYGPITTISVSPLNSQVIYAGTDDGFVWRTTDGGQEWADISSGLPLRW
                     ITRVAADPYDEEVVYVTLSGYRYDSSLPHVFRSADNGVTWDDISGNLPEAPVNDIIID
                     TALDSTIYLATDFGVFVSRNLGQGWQMLGANLPNVPIVDLDFHQPSRTLIAATYGRSM
                     YTFDVDQTVGVSNLNDLPEQILLYPNPAKDYINLNHRFNENIHYSINDLSGKEVKKGR
                     LAAKYERHSLIINDLKPGSYIFKLAGHNRIITGRFEKL"
     gene            complement(4639..>5805)
                     /locus_tag="PKM34_04865"
     CDS             complement(4639..>5805)
                     /locus_tag="PKM34_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82952.1"
                     /translation="GLPTIKVPDIVIQERECDLVIATFGRGIYILDNYSPLRQLKKET
                     REKEAFIFPVKDALMFSRDGGYYGQGSNFFYAPNPEYGATITYYLKDVPKTLKDIRKE
                     KESKLAEEKKPIPIPTLEEMRAEENELPPYLLFTIKDMSGNTIRKMAASPSKGTSRMT
                     WDLRYANGYNVDLDGDKFNPYSAGNGGFMAMPGNYTVAMAMVFNGEVKELAGPVEFTA
                     RVLNNTTLPNADRAGLVAFQDKVSRMINAVNAADEVAKELMKRTQYARQSIQNTPGAP
                     FGMMEAAAALEKKLGDILWALHGEEPKASDEENRPAPPPVMMRLGAIMEASWGNTSSP
                     TQTQRDQLALLEEELPPLLQQLRQIAETELKDLESELDKLGAPWTPGRIPEWKK"
BASE COUNT         1457 a         1431 c         1427 g         1490 t
ORIGIN      
        1 ccgaactggc cggttgacca ggagaagaaa tcggcatcgc agtttgcatt cagagtaatt
       61 gttgagccat tgcagatcaa agtatcattt ccgaggaggg gaagagggga aggggagatt
      121 tcgatttccc gagatgtgtg ttcatatctc cccgagggat accagacatc gacctttacc
      181 tcatgaacac cgggattttt aaaagtatag gtcggtgaaa gttgccatga ggagcttcct
      241 ggtccgtcat caaagttcca ttgaatagtc tggggagtag gaatgaagtt aggcttgaac
      301 tggatcgggt aaccctggca tgtctcgcca atccattcga agcgaaagag gtagtcgacg
      361 aggatattta taaaactata gacgcactca ttagggtaaa tactcactgc attttcctca
      421 tacatgcaac ctgtaccccg aatccagggt ttatgtatga cgcttagata agtttgatca
      481 gcattcgggc ctggctcttc ctttgggccg caatagattt tcccatccct tgccaactgc
      541 atgctatgga gatttgcatt atgataaatt ttcaatgcgg aatttataaa ttcagccgaa
      601 tcagtaacat actgcatatc atattgatat agtacagtag tatcaaaatt tgtaaaataa
      661 gatatatata aatatttgga atctggtgaa aactctaatc cttccgccgc ttcatttagc
      721 tcttcaaccg ggcctttcat agtatacagg caggacacct ctccatttag agcattaaat
      781 ccgcatattt ctatattttg gtgatagctt gaaaccaaat atcttttatc ggatgaaatc
      841 ttaatatatc cccaatggtt ctcacggtca ggcatgacgg agattacagg atttggattt
      901 aaaccgttat ggtccaccag gaatgctgca tatgcatcct caatatattt cctggttatc
      961 acccatacag catccgaatt ttccttcctt actatggcga cccggtcgct gacatcccaa
     1021 ccggcatcaa tttcaatatt tttatccaga acgactcctc ctaaaccctg gtttatatcg
     1081 ataatagaat aatataatcc atattgaggc catccggact ccattgcagt tatgtaataa
     1141 taaataccag cacggcccgg ccattcaacg attgctgcac ctgaaaggcc cggccgaagc
     1201 atttcggtac cattgggcat aataccgtaa cttgtaaaaa tattttcgtt atctgcacta
     1261 aataaataat tgcctgatga atcgcatatt gtcgaaccta tgtgaccatt aagtacctgt
     1321 gaatcatcta gaacaacagg ctctccggaa ttaaaatcca gcccgcacag ctgaggaaaa
     1381 taccagatat tggcttcgtt ctgggcgtag atgccgtttg ataataggag cgacaggact
     1441 ataagacggt aggacggtag gaagaaatga cgataggacg gtaagacggt aagacaatag
     1501 gatctaagga atgcaggaaa gtgaaaattt aatttcaggg ttttcatctt acatcattat
     1561 gaagattgtt gtggatgaaa tgattggcat aaaagtagga tgtttagggg agaaatgcaa
     1621 gggaaaagtc ggctatcgtc tgccggcagt tccctcgcta cgcttgccaa tgccaagtta
     1681 gtaaatttct tctgaattgc tctccggttg agagccctat ttataacaaa aaggacggga
     1741 aagaaaaaaa gcccctccct ttggaaatat tggaatattg cgaaaaacct tcacagggag
     1801 gggttgggga gggaaaccag aaatgcattt tcccctcccc ttaatcccct cccatgctga
     1861 tattaccttg aaaaactgga ttatcgcggg aggggaaaat tgtgcgggtg ctatctcctt
     1921 tgctacaaat gtggaactcc tccggagtaa tgaaaagcta atgtcatcca atattgtgga
     1981 gtttgtaaaa tttacagttt ttcaaatctt ccggtgataa tacggttatg gcctgctaac
     2041 ttaaaaatat aactacccgg cttcagatcg ttgataatca gagaatgtct ttcgtatttt
     2101 gccgctaatc ttcccttttt gacctctttt ccgctcaaat cattgattga ataatggata
     2161 ttctcattaa aacgatggtt taaattaatg taatccttag cgggattagg atagaggagg
     2221 atttgttccg gaagatcatt taaattactt actccaaccg tctgatccac atcaaacgtg
     2281 tacatggaac ggccgtaggt agcggcaatc aaggttcggg agggctggtg gaaatcgaga
     2341 tcgacgatgg gaacgttggg gaggttagca cccagcattt gccagccctg gcctaggttg
     2401 cgcgaaacaa atacgccgaa atcggttgcc aggtaaatcg tcgaatcgag cgccgtgtca
     2461 atgatgatgt cgttgacagg ggcttcgggc agattgccgg agatgtcgtc ccaggtgacg
     2521 ccgttgtcag cgcttctgaa cacatgcggc aaactgctgt cgtaccggta tcccgaaagg
     2581 gtaacataaa caacctcttc atcataagga tcggcggcta cccgcgtaat ccagcgcaac
     2641 ggaaggccgg aggagatatc ggcccattcc tgtccgccat cggttgtacg ccatacaaaa
     2701 ccgtcgtcgg tcccggcata aatcacctgg ctgttcaatg gtgagaccga aatggtcgta
     2761 atcggcccgt aaacaaccga tccgctgccg ctgcccccgg tgagatcggg gctgaccgcc
     2821 tgccaggaga cggcccggtt cgtcgatcga taaagccggt tcgcgccata gtaaaggatt
     2881 tgaggatcag atggatcgaa aaccaccggc gtattccagt tcttccggtc tgagccgctg
     2941 ataccgctca tggccgatga aaaactgctg cccccgttgg tcgaacgggc caggttgccg
     3001 tactggtatt cggcatagac ataactgtta ttcaccggat cgacgagcac atgaaaaccg
     3061 tcgccgccat aaatcatcat ccagttatcc aaagccccgg tataggtccg gttggtgccg
     3121 ttatcctgcg ttccgccata gagacggaac gggtactgtt catccgcctc gcaggtataa
     3181 aactgggtga tgggcaggtt atcacagaaa tcccagttat atccgccatt ataggagaaa
     3241 tagaccccgc cgtcgtttcc cagcaccagc aaatctgtgt tatctggatg aatgacgata
     3301 tcgtgctgat cgacatgtac atcggaggaa atatatgacc agttgtttcc gccgtcgatg
     3361 gttttgtaaa gatcaaagcc gatcacatag gcaacatcag ggttctgcgg atcaacggcg
     3421 atccggccga accaccagcc gtaggaggca tagcaatcgg ccagttcttc atcgctggta
     3481 cggaaccagg aattgccgcc gttcaccgtt ttgtacaatc catcgaaata gccgatatta
     3541 tccgcataaa tggcatagag aatattcgga tcagatgctg aaatatcaat gccgatgcgg
     3601 cctacctgtg cggatgcagc gggaaggccg ttagtcagca gcgtccaggt atcgcctccg
     3661 tcgtaactcc ggtagatacc gctgccgggc ccgccatagt ccctccggtg cggtcggcgc
     3721 accctttccc acatggcggc gtaaagggta tcgggcttcg ccggatgcat gacaatatcg
     3781 atagcgccaa ccgaatcgtt gatgtaaagg acctggtccc aggtaccgcc tccgtcgctg
     3841 gttttaaata ctccccgctc cggattatcc gagaacaaac ggcccattgc cgccacataa
     3901 caggtttggg gatcatccgg gtgtacaacc aatcggccga tgctccccga attattgagg
     3961 ccaaccgggt tccaggaaag ccctgcatcc tccgaaagat agatcccgtt gccgtcgtat
     4021 gccatggaac cgccgccggc attggcttcg ccggttccga cccagatcac cgaggtatct
     4081 gacggggcaa tggcaatatc gccgatggaa aggctcatgg cctcatcgaa tatcggcctc
     4141 cagctttgac ccaggtcaga tgatttgaaa atgcccccgg atgctgcgcc ggcgtaaacc
     4201 gattgggttc cgtaagtgat catggccatc gccgcaatcc gcccgccgat gttcaacggg
     4261 ccttttaaaa gccacccctc acccctttcg tcgctctttt tccgtagtgt ttgcgactgc
     4321 ttccaggcca ttgccctggc atcgtaatta atttccgggt acggaaagga acgctgaagg
     4381 aagaaccagt cgttcgggat tttcccggtt tcgggtgcag gtttattgat gataagatga
     4441 atcgctataa cggcagaaac cagcgtagca actaagagga atagtaatgc taaatacttc
     4501 attttgtttt tcttcgtgga tcttcgtgta tccttcgtga acttcgtgaa atactttagt
     4561 ttaaaaactt gttacacgaa gatcacgaag aagtcacgaa gttacacgaa gttgctcaat
     4621 cagagcaatc tgatgcattt atttcttcca ctccgggatg cggccgggtg tccagggggc
     4681 gcccagtttg tcgagttccg attccaggtc tttcaactcc gtttcagcga tttgcctcag
     4741 ttgctgcagc agcggcggaa gctcttcttc aagcagagcg agctgatcgc gctgggtttg
     4801 cgtcggtgag gaggtgttgc cccaggaggc ttccatgatt gctcccagcc tcatcatgac
     4861 cggtggcggg gcggggcggt tctcttcgtc gctggctttc ggctcttcgc catgaagcgc
     4921 ccaaaggata tcgcccagct tcttttccag ggccgcagcc gcttccatca tgccaaacgg
     4981 ggcgcccggc gtgttctgga tcgattgcct ggcatactgt gtccttttca tcagctcttt
     5041 ggccacttcg tcggctgcat tcacggcatt gatcatccgg gaaaccttat cctggaaagc
     5101 gaccaggccg gccctgtcag cattcggcag ggtcgtattg ttcagcacac gggcggtaaa
     5161 ctctaccggc ccggccagtt ctttcacctc tccgttgaaa accatggcca tcgcaaccgt
     5221 ataatttccc ggcatggcca taaatccgcc attaccggcg ctgtatggat tgaatttgtc
     5281 cccgtcgaga tcgacgttat agccgttagc atagcgcaaa tcccacgtca ttctcgaggt
     5341 gcctttggag ggcgatgccg ccatcttcct gatggtattt ccgctcatat ctttaatcgt
     5401 gaaaagaaga tacggcggca attcattctc ctcggccctc atctcttcca gggtcgggat
     5461 cgggatgggc ttcttctctt ccgcaagttt cgattctttt tctttcctga tatccttcaa
     5521 tgtttttggc acatctttga gatagtaggt gatcgtggcg ccgtattccg ggttgggagc
     5581 ataaaagaaa ttggatccct ggccgtaata acccccgtcg cgggaaaaca tcagggcatc
     5641 cttgaccggg aagataaagg cctccttctc gcgggtctcc tttttcaact ggcgaagcgg
     5701 ggaatagtta tccagaatgt agataccgcg gccgaaggtg gcaatgacga gatcgcattc
     5761 cctttcctgg atgacgatat cgggcacttt gatcgtgggc aggcc
//
LOCUS       DAOQXS010000128         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14179_length_5784_cov_4.451213, whole genome shotgun sequence.
ACCESSION   DAOQXS010000128 DAOQXS010000000
VERSION     DAOQXS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14179_length_5784_cov_4.451213"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..756
                     /locus_tag="PKM34_04870"
     CDS             217..756
                     /locus_tag="PKM34_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNQ82953.1"
                     /translation="MKRTILAALTMLLLMSAQMIYAQNSSALALFDKYGGKEGYTTVS
                     ISKELFGMFADIESDDPDMKEVKTMMEQLEGIKILMFETENADDAELARFKSEVGKLQ
                     VTGYSELMVVKQTGEEVKFLAQKKGDKIGELLLVINGEKEAGFITISGLIDMSTIGKL
                     SKTMNFEGMDNLEKLNDNK"
     gene            779..1321
                     /locus_tag="PKM34_04875"
     CDS             779..1321
                     /locus_tag="PKM34_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNQ82954.1"
                     /translation="MKKLTLIILVAVLPVFAMAQKTPFTQLYDKYVSEPGIQTTQILP
                     GSTSFEWEKTADNTAIREMLNSIESIRIMKIQSDSTKVSPEKIWKNMQKAASEDAYRE
                     ILTISADKVQMRMLMLRGEEGRTREVALIAREGDGVFMMTMTGNMDFSSMFSPENMKN
                     FREIGEYYLENHSGCNKAEE"
     gene            1550..4192
                     /locus_tag="PKM34_04880"
     CDS             1550..4192
                     /locus_tag="PKM34_04880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ82955.1"
                     /translation="MDIPSKYNPKEIEEKWYRHWMDNKYFSSKPDEREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDILVRRARMQGKNAEWLPGTDHASIATEAKVVASLRAKGIDK
                     FDIGREKFLEYAWEWKEKHGGIILEQLKKLGASCDWDRTRFTMDPALYESVINVFIDL
                     YNKGLIYRGVRMVNWDPRALTALSDEEVIYKEVNSKLYYIRYFIENSNNEFITIATTR
                     PETILGDTAVCVNPNDERYKGLAGKKVLVPLLNRPVPVIADEYVDMEFGTGCLKITPA
                     HDINDYEIGRKFKLESIDIFNDNGTLGEKAILYVGEDRFVVREKITRELGDKGHIVKI
                     EDYANKVGYSERTDEVIEPKLSLQWFMKMDVLAKPALDAVMNEDVKFYPSKIKNSYRH
                     WMENVRDWCISRQLWWGQRIPAYYLPNGDFIVAKSAEVALAKARSEFNMPGLKPEDLK
                     QDEDVLDTWFSSWLWPISTFDGIRHPENEDIRYYYPTNDLVTAPEILFFWVARMIMAG
                     LEYRGEVPFRNVYFTGIVRDKLGRKMSKSLGNSPDPILLMDHYGADGVRIGMLFMSPA
                     GNDLPFDEALCEQGRNFSNKIWNALRLVKGWQVDPGLETPETNRASTAWFENKLNQTL
                     ALIDDHFLRFRISDALMAAYKLTWDEFCSWYLEMIKPEYQKPIDRETYEKTISFFETL
                     MKLLHPFMPFITEDVWHRLADRPTGDDIIIAPFPVAGEYHDELLKHFDREREVIMAIR
                     NERNAKGIPNKDKIHLRVKKNHGQEPDTYFDCVVKKMSGLADLEYVEEKVPGALTFIV
                     GSTEFYIPVEEEKIDIPAEIARLETDLEYARGFLATVMAKLSNEKFVNSAPEKVVEME
                     RKKQSDAESKIRVIEAQLLNLRTK"
     gene            complement(4237..4893)
                     /locus_tag="PKM34_04885"
     CDS             complement(4237..4893)
                     /locus_tag="PKM34_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNQ82956.1"
                     /translation="MKIKLGTILVIAFLTLSCTAQKGVQVALLKYSGGGDWYANPTSL
                     TNLVRFCNDNLKMGIEAGYATVEAGSAEIFNYPFVHMTGHGNVFFNESEASNLRNYLL
                     AGGFLHIDDNYGMDPFVRPQMKKVFPELDFVELPFDHPVYHQKFNFPNGLPKIHEHDN
                     KTPQGFGLIYEGRLVCFYSYETDLGDGWEDQDVHHDSDATRQKALQMGANLLSYVFQR
                     "
     gene            complement(4993..>5784)
                     /locus_tag="PKM34_04890"
     CDS             complement(4993..>5784)
                     /locus_tag="PKM34_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNQ82957.1"
                     /translation="MNQPVRIDTLVISTQHDEFDKNPDIMQAKIKEDIRNILIPMALK
                     EMPDRIVRLFDSEYKLYVNPTGNFVIGGPHGDSGLTGRKIIVDTYGGRCAHGGGAFSG
                     KDASKVDRSAAYAARHIAKNLVAAGVAGEVMVQVAYAIGVAEPVGFLVNTSGTAKVKD
                     KNGLIMSDKQISDIAGKLFDLRPYAIIKKFGLQNPVFSPTSTYGHFGRDHYEAEVEVF
                     YEDRDTFRKTVIENEKEVEKIFKKVTFFGWEKLDAVEAIRSAFGI"
BASE COUNT         1642 a         1397 c         1434 g         1311 t
ORIGIN      
        1 cgtatcctct aaattcaacc agggcacccg cagcctgaaa tcggtcaatg ctatcgaaac
       61 aggcctgacc cagcttaaac ctgtcggctc atatgataaa gccgtcagcg aagtgaaccg
      121 cctcagcgac gtcgaaaaag tagaaaaact aatcatcaac aattgaacaa tagaacatta
      181 gaacaattta ataatcaaac aattaattta acaactatga aacgtacaat tctcgcagct
      241 ttaacaatgc tgcttctgat gagcgcacag atgatttatg cccagaatag ctccgctctt
      301 gcgctgtttg ataaatatgg cggtaaagag gggtacacca cggtgagcat ttccaaagag
      361 ctctttggca tgtttgccga tatcgagtcc gatgatccgg atatgaaaga ggtgaaaacg
      421 atgatggaac aactcgaagg gatcaagatc ctgatgtttg aaaccgaaaa tgcggatgac
      481 gccgaactgg cacgcttcaa aagcgaggtc ggcaagctcc aggtaaccgg gtacagcgaa
      541 ctgatggtcg taaaacaaac cggcgaggaa gtgaaattcc tggcccagaa aaagggtgac
      601 aagatcggcg aactactcct ggttatcaac ggtgaaaaag aggccggatt cataaccatc
      661 agcggattga tcgatatgag caccatcggt aaactatcca aaacgatgaa tttcgaaggg
      721 atggataacc tggaaaagct gaatgataac aagtaacagc tcaaaaaaag atcattccat
      781 gaaaaagttg accttaatca tactggttgc cgttttaccg gtctttgcca tggcccagaa
      841 aacgccgttc acccagcttt atgacaagta tgtcagcgaa ccgggtattc agacgacgca
      901 gatcttaccg ggatcgacca gcttcgaatg ggaaaagacg gctgataata ccgcgatcag
      961 ggagatgctg aacagcattg aaagtatccg gatcatgaaa atccaatccg attccacgaa
     1021 ggtttcgccg gaaaagatat ggaaaaacat gcagaaagcg gcatccgaag atgcctaccg
     1081 ggaaatcctc acaatctcgg ccgacaaggt tcagatgcgg atgctcatgc tcaggggcga
     1141 ggagggaaga acccgggaag ttgccctgat tgcaagggaa ggcgacggtg tcttcatgat
     1201 gaccatgaca ggcaatatgg acttttcatc tatgttcagc ccggaaaata tgaagaactt
     1261 ccgggaaatc ggggagtact acctggaaaa ccacagcggc tgcaataagg cagaagaata
     1321 ggtgaaccaa taagagggtg tatcaaaagt ccggtatttc gaaatgattg cgtgacataa
     1381 ggtcgtgctc caacgactaa agccgttggt tccaagaaga ttgcaaagat ggaaccgacg
     1441 gcttcagtcg tcggactaac aataataaac gaagatctgg actttagaga cggcctcttt
     1501 tttttctact tttgccgttt attttcgata ccgaccgata cacaataaaa tggatattcc
     1561 cagcaaatac aacccaaagg agattgaaga gaagtggtac cgccactgga tggataataa
     1621 atatttcagt tcaaaaccgg atgaacggga gccttatacc atagtcatcc ctcctccgaa
     1681 tgtgacgggc gtgctgcaca tgggccatat cctgaacaac accatccagg atatcctggt
     1741 caggcgtgcc cggatgcagg ggaaaaacgc cgaatggctg cccggtaccg accatgcctc
     1801 tattgctacc gaggccaagg tggtggccag cctcagggct aaagggatcg ataaattcga
     1861 tatcggacgg gagaaattcc tcgagtatgc ctgggaatgg aaagaaaaac acggcgggat
     1921 catcctggag cagctcaaaa aactgggcgc ttcatgcgac tgggaccgga cccgctttac
     1981 gatggacccg gcactctatg aatctgtcat caacgtattc atcgatctgt acaataaggg
     2041 gttgatatac aggggtgtaa gaatggtgaa ctgggacccc agggcactga ccgctctatc
     2101 ggatgaagaa gttatttaca aagaggtcaa ttccaaactg tattatataa gatattttat
     2161 tgaaaactca aataatgagt ttattacgat cgcgacaacc cgtcccgaaa ctatccttgg
     2221 cgatacagcc gtttgtgtga acccgaatga tgagagatat aaaggcctgg ccgggaagaa
     2281 agtcctggtc cctttgctca accggccggt cccggtcatt gccgatgaat atgtggatat
     2341 ggaattcggt acgggctgcc tgaagatcac gccggctcac gatatcaacg attatgagat
     2401 cgggaggaag ttcaagctcg aatcgatcga tatattcaac gacaacggca ccctgggcga
     2461 aaaagccatc ctttacgtgg gcgaagaccg ctttgtggtg cgtgaaaaga tcacccgtga
     2521 acttggtgac aaaggccata tcgttaagat agaagactat gcgaacaagg tgggctattc
     2581 ggaaaggacc gacgaggtta tcgagcccaa attgtcgctc cagtggttca tgaagatgga
     2641 tgtactggca aaaccggcgc tggatgcggt gatgaatgaa gatgtgaaat tctacccgtc
     2701 gaagatcaaa aattcttacc gccactggat ggagaatgtg cgcgactggt gcatcagcag
     2761 gcaattgtgg tggggccagc gtatcccggc ctattacctt ccaaacggcg attttatcgt
     2821 ggctaagtcc gctgaagtag ccctggccaa agcccgttcg gaattcaata tgcccggatt
     2881 gaaaccggaa gacctgaaac aggatgagga tgtactggat acctggttct catcgtggct
     2941 ttggccaatc tccactttcg acgggatcag gcaccctgaa aatgaagata ttcgttatta
     3001 ttacccgacc aacgacctgg tgaccgcccc ggagatcctc tttttctggg tagcccgtat
     3061 gatcatggcg ggattggaat accgggggga ggtacccttc cggaatgttt acttcaccgg
     3121 gatcgtccgc gacaagctgg gacgcaaaat gtcgaaatcg cttggcaatt cacccgatcc
     3181 gatcctgctg atggaccact acggagcaga tggcgtcagg atcggcatgc tcttcatgtc
     3241 gcccgccggc aacgacctgc cttttgatga agcattgtgc gagcagggga ggaacttcag
     3301 caacaagatc tggaacgccc ttcgcctggt gaagggctgg caggttgatc ccggcctgga
     3361 gactccggaa acgaaccggg cttcgacggc atggtttgaa aacaagctca accagaccct
     3421 ggccctgatc gacgaccatt tcctcaggtt ccgtatttcc gatgccctga tggcggccta
     3481 caagctgacc tgggacgagt tctgttcgtg gtacctggag atgatcaaac cggaatacca
     3541 gaagccgatc gaccgggaga cctatgaaaa aacgatctct ttcttcgaaa ccctgatgaa
     3601 gctgctgcat ccattcatgc cttttattac tgaagatgtc tggcaccggc tggccgaccg
     3661 ccccaccggc gacgatatca tcatcgcacc tttcccggtg gcgggagaat accacgacga
     3721 gcttttgaag catttcgaca gggaacgcga agtcatcatg gccattcgga acgaacggaa
     3781 cgcaaaaggc atcccgaata aagacaagat ccatctccgg gtcaaaaaga accacggcca
     3841 ggaaccggat acctatttcg actgtgtcgt caaaaaaatg tcgggacttg ctgacctgga
     3901 atatgttgag gaaaaggtac ctggagcgct gacttttatc gtcggttcaa ccgagtttta
     3961 catcccggta gaagaggaga agatcgatat cccggcggaa attgccaggc tcgaaactga
     4021 tcttgaatat gcacgcggtt tcctggcaac ggtaatggct aaactcagca atgaaaagtt
     4081 tgtgaacagc gccccggaaa aagtggttga gatggaacgc aaaaaacagt ccgacgccga
     4141 atcaaagatc agggtgatcg aagcccaatt gttgaattta cgtacaaaat gaattacggt
     4201 aaattgcagg atcgttgcat gcaacgatcc tgcaatttac cgctgaaaca catagctaag
     4261 cagattcgcc cccatttgca gcgccttttg ccgcgtggca tccgagtcgt ggtgcacatc
     4321 ctggtcttcc cagccgtcgc ccaggtcggt ctcataagaa tagaaacaaa ccagccggcc
     4381 ttcgtaaatc aacccgaatc cctgcggtgt tttattatca tgctcatgga tcttgggcaa
     4441 accgttggga aaattgaatt tctgatggta gaccgggtgg tcgaacggga gttccacaaa
     4501 atcgagctcg ggaaagacct ttttcatctg cggcctgaca aacgggtcca ttccatagtt
     4561 atcgtcgata tgcagaaagc ccccggcaag caagtaattc cgcaggtttg aagcttcgct
     4621 ttcgttgaaa aaaacattcc cgtggcctgt catgtgtacg aacggataat tgaagatctc
     4681 tgcgctgccc gcttcaaccg tagcataacc ggcctcaatg cccatcttca ggttatcgtt
     4741 gcagaaccgg accaggttgg tcagcgacgt aggattggca taccagtcgc ctccgccact
     4801 gtacttcaaa agagcaacct ggactccttt ctgagcagtg caggataatg ttaagaatgc
     4861 aatgaccagg atggtaccta acttaatttt catcagtttt caatgaaaaa cataaaagga
     4921 aaaatgccat gaaaaatgaa aaaggcaaaa cgcaaaatgt aacatttatt gtcaaatgaa
     4981 caatgccaga tatcaaatgc caaacgcgga gcggatcgct tccactgcgt caagcttttc
     5041 ccaaccaaag aatgtcacct ttttgaagat cttttcaacc tctttttcgt tttcaatcac
     5101 tgtttttctg aaagtatccc tgtcttcata aaacacttcg acctctgcct cgtaatgatc
     5161 ccggccaaaa tgcccgtagg tactggtagg gctgaaaacg gggttctgca agccgaattt
     5221 tttaatgatg gcataagggc gaaggtcaaa taacttaccc gcaatatcgg agatttgttt
     5281 atccgacatg ataaggccat tcttatcttt aacttttgcc gttccgctgg tattcaccag
     5341 gaaacccacc ggttcggcga caccgatcgc ataagccacc tgcaccatga cctctccggc
     5401 aacacctgcc gcaacgaggt ttttagcgat gtgtcttgct gcatacgctg ccgaacggtc
     5461 gactttcgag gcgtctttcc ccgaaaatgc cccgcccccg tgggcgcaac ggccgccgta
     5521 agtatcgaca atgatctttc ttccggtcaa gcccgagtcg ccgtgcggtc cgccaatgac
     5581 gaaattgccc gtcggattga catagagctt atactccgaa tcgaacaacc tgacgatccg
     5641 gtcaggcatc tcttttaaag ccatcgggat aaggatattc cgaatatcct ctttaatctt
     5701 cgcctgcata atgtccggat ttttgtcaaa ttcgtcatgc tgcgttgaga taaccaaagt
     5761 atcgatcctg accggctggt tcat
//
LOCUS       DAOQXS010000129         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14181_length_5784_cov_3.284866, whole genome shotgun sequence.
ACCESSION   DAOQXS010000129 DAOQXS010000000
VERSION     DAOQXS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14181_length_5784_cov_3.284866"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..720
                     /gene="clpP"
                     /locus_tag="PKM34_04895"
     CDS             37..720
                     /gene="clpP"
                     /locus_tag="PKM34_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNQ82958.1"
                     /translation="MKADEFRRYAVKHRGMSGLAIDQYTSIVGNYISPTIIEERQLNI
                     ATMDVFSRLMMDRIIFLGVPIDDYVANIIQAQLLFLESVDPAKDIQIYLNTPGGMVYA
                     GLGIYDTMQYINPDVATICTGMAASMGAVILCAGAKGKRTALKHSRILIHQPMGGASG
                     QASDIEITAKEIQKIKKELYEIIAMHSGQDYKKIWKDADRDYWMMAEEAKEYGMIDEV
                     LDRNKNKSV"
     gene            721..1947
                     /gene="clpX"
                     /locus_tag="PKM34_04900"
     CDS             721..1947
                     /gene="clpX"
                     /locus_tag="PKM34_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNQ82959.1"
                     /translation="MQKGSQKCSFCGRDRSEVNMLIAGIDAHICDSCVTQAQAILSEE
                     LVHHKTKSSFDIELKPPVEIKKYLDQYVIGQEEAKRVLSVAVYNHYKRIMQPASDDDV
                     EIEKSNIMIVGETGTGKTLLARTIAKMLKVPFAIADATVLTEAGYVGEDVESILARLL
                     QAADYDVAAAERGIVFIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGAIINVPP
                     QGGRKHPEQKFIQVNTQNILFISGGAFNSIDRIIASRLRTNIIGYQSKDQEAFDKNNL
                     LQYIAPSDIKKFGMIPEIVGRFPVLTYLMPLDHDALKKILTEPRNALVKQFVKLFKMD
                     GITLKFDDSAFEFIVEKATEHKLGARGLRSILEAILTDAMFELPSKGTSKSLEIDRRY
                     AEEKFGKSGLSRLKVA"
     gene            1985..2563
                     /locus_tag="PKM34_04905"
     CDS             1985..2563
                     /locus_tag="PKM34_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNQ82960.1"
                     /translation="MLKFKFSLIFLFVFLKVSSQEQSLIPVGGAIINGDTIAVVQLRE
                     ISIFGWRNMSPAEERKMTRLIRNVKVAYPYARLAGLKFIEYEDILSQTPDENRRRKIM
                     KQVEDELMDEYGEELKKLTISQGKILIKLVDRETGESFYDIVTEFRGEFRAIFYQAFA
                     RFFGLNMKEKYDPEGNDREIENIVKMIETGQL"
     gene            complement(2571..3992)
                     /locus_tag="PKM34_04910"
     CDS             complement(2571..3992)
                     /locus_tag="PKM34_04910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ82961.1"
                     /translation="MNKKLQVIRYLLADLFSAALAWSFFFIYRKYSIEPVTFENLKTI
                     LQDTNYFLGVVIVPAFWIFLYFILGTYNENKIFRKSRLREMSQTLFISLVGVIIIFFA
                     LILDDAISTYKSYYQLFAVLYLLHFGFTYLFRLLLTSRIVRKIHKKQLGFNTIIVGSN
                     GNAVKIFEEIEHQELPSGNKFIGFVSVKDYDHFKVARYLPYLGKFQDLKRIIAENQVE
                     EVILAIERSERETIRTILTMLEETDVVIKIIPDMQDYLMGMVKMSSIWHTPLIQVSQD
                     LMPPWQQSIKRFIDIIAALIALIILFPVFVIIAIGIKIGSKGPIIYSQERVGLHGHAF
                     KMHKFRSMYSDAERHGPQLSKKDDSRITRIGKFLRKVRLDEVPQFYSVLKGDMSLVGY
                     RPERQYYIDKIVEKAPEYRLLFKIKPGITSWGQVKYGYAENVEQMVERLKYDLLYIEN
                     MSLAMDFKIMIYTVLIVVQGRGK"
     gene            complement(3992..4912)
                     /locus_tag="PKM34_04915"
     CDS             complement(3992..4912)
                     /locus_tag="PKM34_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82962.1"
                     /translation="MRSLNRKASLPNILIWFLFSIFIITLTGCDKFEGDQTVPSYLKI
                     DSILFISDNELEGTSNQKISDAWVFIDDDLIGGFELPALIPILKKGNHKLEVRPGIIL
                     NGISDTRAPYPCIQPVIIESLELFPDSIVTLSLTSNYYENSEFVWMEDFEDASLAIKA
                     SQNSDTAIYRTNGNLAYHDVYSEFSGICYVDTANSFVQLVSVESNNQGFVFDRGDFIF
                     LEINYRNNIPIVVGVYIKLMDNTVQQRSFLIINPTEEWNKIYVNFTPIVNETTDAVYY
                     NVYLEAQQQDDVENGYIMLDNIKLVTRPNL"
     gene            complement(4957..>5784)
                     /locus_tag="PKM34_04920"
     CDS             complement(4957..>5784)
                     /locus_tag="PKM34_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ82963.1"
                     /translation="IEELIDSVDVVDIVTPTVSHFDCASRALKKARHVFIEKPVVTSP
                     EEARQLMVYADEANVKVQVGHVERFNPAFLAAQPFINKPMFIESHRLAQFNPRGTDVP
                     VVLDLMIHDIDIVLSAVRSNIKKINASGVSVVSDSPDIANARIEFDNGCVANLTASRI
                     SMKNMRKSRFFQRDAYISVDFLKKDVEIIRISDPQDENPDPLAMIIDLGAKRGKKQIS
                     IEKPEVQNINAIKTELESFHDAIVNNTTPPVTINDGYEALSVAYQILDKMKELPVGL"
BASE COUNT         1626 a         1246 c         1350 g         1562 t
ORIGIN      
        1 aatttgtaaa aatcatagca caaaaaagtg aagttaatga aagctgatga atttcggaga
       61 tatgcggtca agcaccgtgg gatgagcgga ctggccatcg atcaatacac atccattgtt
      121 ggaaactaca tctcccccac cattattgag gagcgtcagc tgaacattgc caccatggat
      181 gttttttccc ggctgatgat ggacaggatc atctttctcg gcgtacctat agatgattat
      241 gttgccaata tcattcaggc gcagttactg tttctggaat cggtggatcc ggccaaggac
      301 attcagatat acctgaatac tccgggaggg atggtatatg ccggactggg catttatgat
      361 acgatgcagt atatcaatcc ggacgtggcg accatatgta ccggaatggc ggcatcgatg
      421 ggggctgtca ttttatgtgc cggcgccaaa gggaaacgga ctgctctcaa gcattcgcgt
      481 atcctgatcc accagccgat ggggggagct tctggccagg cttccgacat cgaaattaca
      541 gcgaaagaga tccagaagat aaaaaaagaa ctttacgaga ttattgccat gcattccggc
      601 caggattaca agaaaatctg gaaagatgcc gaccgggatt actggatgat ggcagaggaa
      661 gcaaaggaat acggtatgat cgacgaagtc ctggacagga ataaaaacaa atccgtctaa
      721 atgcaaaaag gaagtcagaa gtgctcattc tgtggcaggg accggtcgga ggtcaatatg
      781 ctgattgccg gaatcgacgc ccacatatgc gattcctgtg tcacccaggc ccaggcgatt
      841 ctcagcgaag agcttgtgca ccacaaaaca aaaagctctt ttgacattga actaaagcct
      901 cccgtcgaga tcaaaaaata cctcgaccag tacgttattg ggcaggaaga agccaaacgg
      961 gtcctttccg tcgcggttta caatcattac aaaaggatta tgcagccggc cagtgatgat
     1021 gatgtggaga tagaaaaatc caacattatg attgtcggtg agacaggtac gggaaaaacg
     1081 cttttggcaa gaaccattgc taaaatgctt aaagtacctt tcgctattgc tgatgcaacg
     1141 gtactgacgg aagccggata tgtgggggaa gacgtggaaa gtatcctggc ccgtttgctg
     1201 caggcggccg actatgatgt ggcagctgca gaacgcggga tcgtattcat tgacgaaatc
     1261 gataaaattg cccggaaagg tgataatcct tccattaccc gggatgtttc gggagaaggg
     1321 gttcagcagg ctttgctaaa actgctggaa ggcgccatta tcaatgtccc tcctcaaggc
     1381 ggaagaaaac atccggaaca aaaatttatc caggttaaca cccagaatat cctttttatt
     1441 tccggcggcg cttttaattc gattgaccgc atcatcgcat cccggttaag aacgaacatc
     1501 atcggttacc agtcaaagga ccaggaagct tttgacaaaa acaacctgct acagtatatt
     1561 gcaccctccg atatcaagaa attcggaatg attcccgaga tagtaggacg ttttccggta
     1621 ctgacttacc ttatgcccct ggatcatgat gcgttgaaaa agatccttac tgaaccccgc
     1681 aatgcccttg tcaaacaatt tgtcaagctt ttcaaaatgg acggcataac attgaaattt
     1741 gatgacagcg cctttgagtt catcgtcgaa aaagccacgg aacacaaatt gggagcccgg
     1801 ggattacgat ctattcttga agccatttta accgatgcca tgttcgaatt gccctccaaa
     1861 ggcacttcaa agtcattgga aattgaccgc cggtatgctg aagaaaaatt cgggaaatcg
     1921 ggattatcca ggttgaaagt ggcctgattt ttctgtggca taattgttgg tttttaaatc
     1981 gactatgctg aaatttaaat tctcattgat tttcctgttt gttttcctga aggtgtcgtc
     2041 tcaggaacag agcctgattc ctgtcggggg ggccatcatc aacggggata ccatcgctgt
     2101 tgttcagctc agggaaatca gcatttttgg ctggaggaac atgagcccgg cagaagaacg
     2161 taagatgacg cggctcatcc ggaatgttaa agtcgcatac ccatatgccc ggctggccgg
     2221 tttgaaattt atcgaatacg aggacattct ttcacaaacc ccggatgaaa atagaagacg
     2281 aaagatcatg aagcaggtgg aagacgaatt aatggatgag tatggagagg aattgaagaa
     2341 gctgaccatt tcacagggaa agatactgat taagctggta gaccgggaaa ccggcgaatc
     2401 cttctatgat attgtcactg aattcagggg agaattccgg gctatttttt accaggcctt
     2461 tgcacgtttc tttggcttga acatgaaaga gaaatatgat ccggaaggga atgaccgtga
     2521 gattgagaat attgttaaaa tgatcgaaac agggcagctt taaaaggaaa ctatttcccc
     2581 ctaccctgga ctacaattaa gacagtgtat atcatgatct taaagtccat ggccagcgac
     2641 atattttcga tatacagcag gtcatatttc aatcgttcca ccatctgctc cacattctcc
     2701 gcataaccgt atttaacctg gccccaggat gttatgcccg gtttgatttt aaacagcagc
     2761 ctgtattcgg gtgccttttc cacgatttta tcgatgtaat actgcctttc gggtctgtat
     2821 ccgaccaacg acatatctcc tttgagcacc gaatagaact ggggtacttc atcgagcctg
     2881 acctttctga ggaatttacc gattcgggta atcctgctgt cgtctttctt tgaaagctgc
     2941 ggcccatgtc tctccgcatc ggagtacatc gacctgaatt tatgcatttt aaatgcatgc
     3001 ccatgcaatc cgacccgctc ctgggaataa atgatcggcc ctttggagcc aattttgatt
     3061 ccaatggcaa taatgacaaa tacagggaat aaaatgatca gggctatcag ggccgcaata
     3121 atatcgatga agcgtttgat ggattgttgc cagggaggca taagatcctg agatacctgg
     3181 atcaggggcg tatgccatat ggatgacatc ttgaccatgc ccatcaggta atcctgcatg
     3241 tcgggaataa tcttaatgac cacgtctgtc tcctccagca tggtcaggat cgtccggatg
     3301 gtttctcttt ccgaccgctc aatcgcaagg attacttctt caacctggtt ttcggcaata
     3361 attctcttaa gatcctgaaa tttacccagg taaggcaggt acctggcaac cttaaaatgg
     3421 tcgtaatcct tgacgctgac aaagccaatg aatttgttac ctgaaggcag ttcctgatgt
     3481 tcaatttctt caaatatttt cacggcattc ccattgctcc ccacaatgat cgtattaaag
     3541 ccgagctgct ttttatgaat tttccttact atccggcttg taagtaataa acggaagagg
     3601 tatgtaaagc cgaaatgtaa tagatataag acggcaaata actgataata tgacttatag
     3661 gtgcttatgg catcgtccag gataagggca aaaaatatta ttatgactcc gaccagtgaa
     3721 ataaaaagtg tctggctcat ttcccgaagc ctggatttcc ggaagatctt attttcgttg
     3781 tacgtgccga ggatgaaata caggaaaatc cagaacgcag ggacaataac cacgccgagg
     3841 aaatagttcg tatcctgtaa gatcgtcttc aggttttcga atgtaaccgg ttcgattgaa
     3901 tacttacgat aaataaaaaa gaatgaccag gccagcgcag cggagaagag gtcggccaga
     3961 agatacctga ttacctgtaa ttttttattc atcaaagatt tggtctggtt accagtttaa
     4021 tattatccag catgatataa ccgttttcaa catcatcctg ttgttgagct tcaagatata
     4081 cattataata aactgcgtct gttgtttcat tgacaatcgg tgtaaagtta acatagattt
     4141 tattccattc ttcagtcggg tttattatca ggaatgacct ctgctgcacc gtattatcca
     4201 taagtttgat atataccccg acaacaattg ggatattatt ccggtagttt atttccagaa
     4261 agatgaaatc tccccggtcg aaaacaaatc cctggttatt gctttccact gagaccagtt
     4321 ggacaaaact attcgccgta tctacatagc aaattcctga aaattcggag tatacatcat
     4381 gataagccag atttccattt gtccggtata tggccgtatc actgttctgg ctggctttga
     4441 tggccaggct tgcatcttca aaatcttcca tccagacgaa ctccgaattt tcatagtaat
     4501 tggatgttaa tgacagggtt actatgctat cggggaaaag ttctaacgat tcgatgatta
     4561 ccggttggat acagggatag ggcgcccggg tgtcggagat accgttcaat atgataccgg
     4621 gacggacctc cagcttatga ttaccttttt tgaggattgg aatgagtgca ggtagctcga
     4681 acccgccgat aaggtcatcg tcgataaaaa cccatgcatc ggaaatcttc tggttgctgg
     4741 tcccctctag ctcattatcg gaaataaata aaatggaatc gatcttcagg tatgatggaa
     4801 ccgtctgatc cccttcaaat ttatcgcatc ctgtaagagt tatgataaat atagaaaaca
     4861 ggaaccagat cagaatattc ggcaggcttg cttttcggtt tagacttctc atgtttatcc
     4921 aacggtgaca ggcacgtgat tattgcttcg tttcggttac agacccacgg gtaactcctt
     4981 cattttatcg aggatctggt aagctacgct cagggcttcg tagccatcat tgatagtgac
     5041 cggaggggtt gtattgttga cgatggcatc gtgaaaactt tcgagttcgg ttttaatggc
     5101 attgatattc tgtacttccg gtttttcgat ggagatttgt tttttccccc ttttggcgcc
     5161 cagatcaata atcatagcca gaggatcggg gttttcatcc tgagggtcgc tgatccggat
     5221 aatttctacg tctttcttaa gaaaatcgac ggagatatat gcatcgcgct ggaagaaacg
     5281 ggatttccgc atattcttca tagagatccg gctggctgtc aggttggcta cgcaaccgtt
     5341 atcgaattct atcctggcat tggcaatgtc gggtgaatcg ctgacaacgg aaacgccact
     5401 tgcattgatc ttttttatat tcgacctcac ggcgctgagt acgatatcga tatcatgaat
     5461 catcaggtcg agcacaacgg gcacatccgt tccacgagga ttaaactggg ccaggcggtg
     5521 tgattcgatg aacatcggtt tgttgataaa aggctgtgcg gcaagaaatg ccgggttaaa
     5581 ccgttcgaca tggccaacct gcaccttcac gttggcttcg tcggcgtata ccataagttg
     5641 ccgggcctct tccggtgagg ttacgacggg cttctcgata aaaacatgcc gggctttttt
     5701 cagtgccctg gaagcgcaat caaaatgaga aacagtcgga gtgacaatat cgacaacatc
     5761 aacagaatca ataagttctt caat
//
LOCUS       DAOQXS010000130         5777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14200_length_5777_cov_3.476931, whole genome shotgun sequence.
ACCESSION   DAOQXS010000130 DAOQXS010000000
VERSION     DAOQXS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14200_length_5777_cov_3.476931"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..460
                     /locus_tag="PKM34_04925"
     CDS             203..460
                     /locus_tag="PKM34_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNQ82964.1"
                     /translation="MRAANFTEFRTRLKKFLDDVENNHETLIIKRKTGNGAVLISLDE
                     YNSIMETVHLLSSKINADRLYESIQQMKSGKVVKKDLIEEQ"
     gene            457..720
                     /locus_tag="PKM34_04930"
     CDS             457..720
                     /locus_tag="PKM34_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNQ82965.1"
                     /translation="MKITFSRNAWEDYTSWQSEDKRMLKKINELIKDIQRIPFEGMGK
                     PEPLKYDLSGLWSRRIDREHRLVYQANETELLIYSCRYHYDLY"
     gene            1201..2631
                     /locus_tag="PKM34_04935"
     CDS             1201..2631
                     /locus_tag="PKM34_04935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNQ82966.1"
                     /translation="MGQHLIDKIRRNRGMAIYAVLATYAVLAAFIITFFNGTGDSGDS
                     IHHYLFARYAPQHIELFFDHWAKPLFVLLASPFAQFGFTGMKIFNALAGLFTIWFTYR
                     TVQLLNLRNPLLVTLLLIFAPMYFVQTFSGLTEPLFALFISIGLYFVVREKFVLSFLI
                     ISFLPFIRSEGLILIGVFALYAIVKNQWRPLPWLLTGHILYSVAGFFVHGDLLWVFTR
                     IPYARLASTYGSGTIFHFAEKLNYVIGIPIFILFWAGVLSIILKLVRRQISKEIFILV
                     FLGFLSYFIAHSLFWYFGIFNSMGLQRVLIGVMPMIAIISLTGFNFLTEELARNHRVT
                     RKISQIALMSYILIFPFLSNPAAIKWDTDLSLAEDQVAATGVKEFIISHYSITTPRLI
                     YSHPYLSEALQVDHFDQEKRLDLNKENLSRLRPGDLIIWENWFAVVENGISKESLEQN
                     PDLINIFNTTNNKGKREIQFIIFKKN"
     gene            2770..>4215
                     /locus_tag="PKM34_04940"
     CDS             2770..>4215
                     /locus_tag="PKM34_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ82967.1"
                     /translation="MKEKVVIIGGGVAGLSAGIYSAMNGFDTEIYEMHSVAGGQCTAW
                     DRDRFRFDYCLHWLVGTSKGPFHEIWRETNVLNDQTEIIDHEIHSQIIDNKGERFTIY
                     ADINRWEEYLLKLSPEDEKPIRSMCREMRKSARLEPFTSAPELRSPLEYLRLLPSMIP
                     LMFVVQKFGRLTCAEYFDKLNFKSEKIRTAFHSLYGDRNFSALAFIFMLGWFNQKNAG
                     YIKGGSYPLAQRMRERYESLGGRIVFRKRVEKIIVEKNIAKGIVLTDGTVIKADHVIS
                     AADGFSTIYKMLDGKYTSKQVEHAYKNWELFPPLVQVSFGVGKEVKADAPILLNFSKG
                     VKIGMTELEYGFSLMNYSYDNTMAPEGKTTIVMRYESPWKLWENLEGEAYRAEKEQIK
                     KDAVACLESIHPGISGSIEVIDVATPKTDVKYTGVKDGAYEGFLPTRENMMKGLRMQL
                     PGLKNFYMAGQWLFPGGGLPPSAQTGKWAVQM"
     assembly_gap    4216..4225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4226..4585
                     /locus_tag="PKM34_04945"
     CDS             <4226..4585
                     /locus_tag="PKM34_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82968.1"
                     /translation="VNRPEPTKSMKQTEEIIFNALRTYSNVHRGSGYNSMVTTENYEQ
                     AKLKVLGFMGLDKKSHKVVFCSPRQSQVITSGMKPGSYQVLSSIDTGLAIGLRAITAN
                     EKPFRWYLKLPAEALRA"
     gene            4546..>5777
                     /locus_tag="PKM34_04950"
     CDS             4546..>5777
                     /locus_tag="PKM34_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ82969.1"
                     /translation="MVPKITGGGTTRLISGDWVVWAKAPDRHEAGTPSVINAIAFTSA
                     LDMKKKTNAGELIQIKPENTEPAQDHLVENEYNDLNGEELLAYLRDSVVGKDITVEAG
                     GENVTYLNLDHGASTRAFWPVWEAYRHALNAGPDRRSEITGNTRRIISGFLGAPAEEY
                     DIVFTSNTTEALNLAAESLSLEAGEETEPVVINTVLEHNSNELPWRFAKGIHHERLNV
                     DAQGFIDLDELDTKLSEYNEQGRHGKRRVAIVAVSGASNVLGTCNDLEAIGRITHKYG
                     ARLLVDAAQLMAHRPVDMAGTGIDYLAFSGHKIYAPFGSGALIVRKGLLKFNDQETSQ
                     IHDSGEENLAGIAALGKSLELLERVGMDIVMKEEINLTAQFLEGMNRVPGIRIYGIKS
                     SLDSNFTRKAGVISFDFK"
BASE COUNT         1616 a         1271 c         1387 g         1493 t
ORIGIN      
        1 ttgtccattg tccattgtcg atggccgaag ggaagattcg aaaatcgaac gtcgacaatc
       61 gataatcgac aatcatgtgc ccggcccagg gcgctgcgct cggagcgtta ggctgcatgt
      121 ttattatttt taattttgct tataagtaat taataaagta cgtataattg tactcgttta
      181 ataacttaaa taatttataa aaatgcgagc agctaatttc acagaattca gaacccgctt
      241 aaaaaaattc ctagatgatg tcgaaaataa tcatgaaaca ttgattatta aaagaaaaac
      301 gggcaatggt gcggtattga tctcacttga tgaatacaat tccataatgg aaactgtcca
      361 tcttctcagt tccaaaatca atgctgacag attatatgaa tctattcagc aaatgaaatc
      421 tggtaaagta gtcaaaaagg atttaattga agaacaatga aaatcacttt ttcgagaaac
      481 gcctgggaag actatacttc ctggcaatca gaagataaaa ggatgttaaa aaagatcaat
      541 gagttaatta aggatattca gagaatacct tttgagggaa tgggtaagcc agagcccttg
      601 aaatatgatt tatcaggttt atggtccaga cgaattgacc gagagcatag attagtttac
      661 caagcgaatg aaaccgaatt attaatctat agctgcaggt accattatga tttatattaa
      721 acacgcagcc taacggggcg cttcgctgag cattatatgt tcttaaaaac tttttgtcga
      781 ttcgaaaagt aaaattatta tgtaccttta accgtgaaaa aaatcagtga aatgaacaag
      841 gcaatgatca tctggctggt cctctttttt gggctatcct gtcaggcttt tgcaaaagct
      901 ggtgttgcaa atggcgggtt cgaacttttg ctggcaatca tcggattcct tcttttggtg
      961 acaggattat atacgggaat cgaatatctg ataaaaaacg gccgaaatct gtttaacagg
     1021 ttcagggcga ttttgaaaga ttgaatctac aattcattca gaccttccgg tgattttatg
     1081 gttttcgttt gttcctcccg gataatctga tctcaggata tttataccgg gactgtaaat
     1141 ccgttatcag ctgcaatccc ggttttgata ctcccttcat ttaaatatct ttgtaaaata
     1201 atgggtcaac atctaatcga taaaattcgc agaaaccggg gaatggccat ttatgcggta
     1261 ttggccacat acgccgtact ggcagctttc atcatcactt tttttaacgg taccggcgat
     1321 tcaggcgaca gcatccatca ttaccttttc gcacggtatg ccccgcaaca tatcgaactt
     1381 ttcttcgacc actgggccaa gccactgttc gtacttcttg ccagcccatt tgcccaattc
     1441 ggatttacgg ggatgaaaat attcaatgcc cttgccgggt tattcaccat ctggttcacc
     1501 tacaggacgg tacaactatt gaacctgagg aatccgctgt tggtaacgct attgctgatc
     1561 ttcgccccta tgtatttcgt ccagacattt tcaggcctta ccgagccatt gtttgccctg
     1621 tttatatcta taggcctgta ttttgtcgta agggaaaaat ttgtactttc ctttctgatc
     1681 atttcgtttc tcccctttat ccgttcggaa gggctgatcc tcataggcgt ttttgcattg
     1741 tatgccattg tgaagaatca atggcggcca ctaccctggc tgctcacagg ccatatcctc
     1801 tattcagttg ccggcttttt tgtgcacggc gacctgctct gggtctttac ccggatcccc
     1861 tacgcccggc tggccagcac ctacggcagc ggaaccattt tccattttgc agagaaactg
     1921 aattacgtta tcggcattcc cattttcatc ctcttctggg caggtgtcct cagcatcatc
     1981 ctgaaactgg tccgcaggca aatatcaaaa gagattttca tcctggtttt cctgggtttc
     2041 ctttcgtatt ttattgcaca cagcctgttc tggtatttcg gcatttttaa ttcgatgggc
     2101 ttgcagcgag ttctgatcgg cgttatgcca atgatagcga ttatttccct aacgggattt
     2161 aattttctga cggaagaact agcccggaat caccgggtaa cccggaaaat ttcacagatt
     2221 gcattgatga gttacatcct gatattcccc tttctttcca atcctgcggc gatcaaatgg
     2281 gatactgact taagcctggc ggaggaccag gtggctgcca ctggcgttaa agaatttatc
     2341 ataagtcact acagtatcac cacaccccgg ttgatctatt cgcatcccta tttaagcgaa
     2401 gcattgcagg tcgaccattt tgaccaggaa aagaggctgg atttgaataa ggaaaacctc
     2461 agccggttgc ggccgggcga cctcatcatc tgggaaaact ggtttgccgt agtggaaaat
     2521 gggatctcaa aagaatccct cgaacaaaat cctgatctga taaacatttt taatacaaca
     2581 aacaacaaag gaaaacgaga aattcaattt atcattttta agaaaaattg attgacagta
     2641 aaaagaatag tatcgaaatc ctgttttcat cccggataaa atgaaattca ccggtttatc
     2701 ccgttcattc accggaaaat cattcaggtt taaagcaggt gcagatactt ttgcgcaaaa
     2761 attaatcaca tgaaagaaaa agttgtcatt attggcggag gtgttgccgg gttgtcggcc
     2821 ggaatttatt ccgcaatgaa cggattcgac accgaaatct acgagatgca ttcggtagcc
     2881 ggagggcagt gtacggcgtg ggaccgtgac cggttccggt tcgattattg tctccactgg
     2941 ctggtgggaa cctccaaagg ccctttccac gaaatctggc gggagaccaa tgtgttgaac
     3001 gatcagaccg agattatcga tcatgagatc cattcccaga tcattgacaa caaaggagaa
     3061 aggtttacga tttacgccga catcaaccgg tgggaagaat atttgctgaa actctccccc
     3121 gaggatgaaa agccgatccg gagcatgtgc agggagatgc gtaaaagcgc caggctggag
     3181 cctttcacat cagccccgga actgcgcagc ccgctggaat acctgcgcct tctgccttcc
     3241 atgatccctt tgatgttcgt tgtgcaaaaa ttcggccgac tgacctgcgc cgaatatttc
     3301 gacaaactca atttcaaaag cgaaaagatc aggacagctt ttcattcgtt gtatggcgac
     3361 cggaattttt ccgccctggc gttcatattc atgctgggtt ggttcaacca gaagaatgcg
     3421 ggttacatca aagggggctc ctaccccctg gcccagcgga tgcgggaaag atacgaaagc
     3481 cttggcggca ggatcgtatt cagaaaaagg gtggagaaaa ttatcgtaga gaagaatata
     3541 gcgaaaggaa tagtactgac ggatggcact gtcattaaag ccgaccatgt gatcagcgca
     3601 gccgatggat tttcaacgat ctacaagatg cttgacggca aatacacttc gaaacaagtt
     3661 gagcatgcat ataaaaactg ggaattgttc ccgcccctgg tgcaggtttc tttcggtgtc
     3721 ggcaaagagg taaaagccga tgcccctatt cttctgaatt tctccaaagg ggttaagatc
     3781 gggatgaccg agctggaata tggattctcg ctgatgaatt attcttacga caacaccatg
     3841 gcacccgagg ggaaaacgac catcgtcatg cgatatgaaa gtccgtggaa attatgggaa
     3901 aacctggaag gggaggctta ccgtgcagaa aaggaacaaa taaagaagga tgccgttgcc
     3961 tgcctggagt cgatccaccc cggtatatcg ggctccatcg aagtgataga tgtagccaca
     4021 cccaaaacgg atgtcaaata cacaggtgtt aaagacgggg cctatgaggg atttctgccc
     4081 accagggaaa acatgatgaa aggattgaga atgcagttgc cgggattgaa gaacttctac
     4141 atggccggcc agtggctgtt cccgggaggc ggactgccac cgtcggcgca gaccggaaaa
     4201 tgggcagtac agatgnnnnn nnnnngttaa cagacctgaa ccaaccaaaa gtatgaagca
     4261 aacagaagag atcattttca atgccttgag gacttactcg aatgttcacc gggggagcgg
     4321 atacaattcg atggtgacca ctgagaatta tgaacaggct aagctcaaag ttctcggttt
     4381 catgggtttg gataagaaga gccataaggt ggttttttgt tcaccccgcc agtcccaggt
     4441 cattacctcc ggcatgaagc cgggcagtta ccaggttctt tccagtatcg atacaggcct
     4501 ggccatcggc ctccgggcta ttaccgccaa cgaaaagccc tttcgatggt acctaaaatt
     4561 accggcggag gcactacgcg cttgatttca ggcgactggg tcgtttgggc aaaggcgcct
     4621 gatcgccatg aagcgggaac accatcagta atcaatgcta tcgcttttac gtcggcattg
     4681 gatatgaaaa agaaaaccaa tgccggtgaa ctaatccaaa ttaaaccgga gaatacagag
     4741 ccggcacaag atcacttggt ggaaaacgag tataatgacc ttaatggaga ggaattgctg
     4801 gcatacctga gagattcggt tgtcggaaag gatattaccg tggaagcagg cggagaaaat
     4861 gtgacctacc tgaaccttga ccacggcgcc agcacccggg ccttttggcc ggtctgggaa
     4921 gcataccggc atgcgttaaa tgcaggcccg gaccggagat ctgaaattac cgggaatacg
     4981 cggcggataa tctccggttt tctcggcgcc ccagcagagg agtacgatat cgtgttcacg
     5041 tccaacacaa cggaagcatt gaatctggct gcggaaagcc tttcactgga agctggtgaa
     5101 gagactgagc ccgtggtgat caacaccgtc cttgagcata attccaatga gttgccatgg
     5161 cgttttgcaa agggtatcca tcacgaacgg ttaaatgttg atgctcaagg ttttatcgat
     5221 cttgacgaac tggatacgaa attaagcgaa tacaatgagc agggtcgtca tggaaaacga
     5281 agagtggcaa tagtagcggt cagcggcgcc tcgaatgtgc tggggacgtg caacgatctg
     5341 gaggcgatcg gtcggattac gcataagtac ggtgcacgtc tgcttgttga tgcggcgcaa
     5401 ctgatggcac accgcccggt cgatatggcc ggcaccggta tcgactacct ggctttctcc
     5461 ggccataaga tctatgctcc gtttggcagc ggcgctttaa tcgtgcgcaa agggctgcta
     5521 aaattcaatg atcaggaaac atctcaaatc catgattcgg gcgaggagaa ccttgcaggc
     5581 attgccgcac tgggtaaatc cctcgaattg ctggaacggg tgggtatgga tattgtgatg
     5641 aaggaagaaa taaatctgac agcgcaattc cttgaaggga tgaaccgggt tcccggcatc
     5701 cggatttatg gaataaaaag tagtctcgac agtaatttca ccaggaaagc cggtgtaata
     5761 tcatttgatt tcaagaa
//
LOCUS       DAOQXS010000131         5773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14209_length_5773_cov_3.965373, whole genome shotgun sequence.
ACCESSION   DAOQXS010000131 DAOQXS010000000
VERSION     DAOQXS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14209_length_5773_cov_3.965373"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKM34_04955"
     CDS             <1..1088
                     /locus_tag="PKM34_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ82970.1"
                     /translation="ETEQSIRDRIAANKKKITELNQEIAGLEKQLGDLNNDDNSSENI
                     AVEVMDLTLSSFSHYIQVSGTAEAVRQAYISPEVSGQIREVYVKEGDYVEKGKLLAKL
                     NSEVTESSIADLQTSLDLAGVVFEKQKRLWEKGIGSEIQFLNAKNNKESLEQKMQTLR
                     AQLDMALIKSPVSGIVDKIYLKKGELAMPGVQLMQVVNLDELYINADVSETYLSDVRE
                     GDDVKVEFPVFPDKTLETPIYRKGNVINPNNRTFQIQLKLPNPDKMLKPNILSVIHIN
                     DFFADSAIVVPSSIIKQDISGSYLYAIMETGNKWTAKKVYIKTGKSYLDKTMVTDGLK
                     EGMKVITMGYNLVSDGTEVTVKQENAS"
     gene            1078..2691
                     /locus_tag="PKM34_04960"
     CDS             1078..2691
                     /locus_tag="PKM34_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNQ82971.1"
                     /translation="MHLNAAHISRNLFTALIIITAALAPVFPLFAQKSTIVHIENADL
                     LRVAKINGMSVKTLKGNAILRQDSTYFYCDTASLDENNNLNAMGRVHINYGDSVHLYG
                     DYLTYSGNTRIAIMDSNVRMVDKRATLYTDHLEYDRNLAVAYYFTGGKIVDDDNVLTS
                     IRGKYLTRLNEFEFSDSVVATHPDYTMYSDTLRYNTETEIVYIEGPTNIYGKEDHIYS
                     EKGWYDTRSDLAELNQNNRIRHLEQILKGDWIYYDRENEYGKARGHVWVMDTVQDIIM
                     EGGIGEFHRSEKYSYITDRARAILIDGSDSLFMHADSFIMVMDSADEARYLFAYHRMK
                     FYKQDLQGLCDSLVYRVQDSVIALLKNPVLWSDDNQLTADSMWMHISDNRIDSMVLFN
                     MAFIVSRDSVETYNQIKGKQMRAYFRDNELYRIKVLGNAETIYYVRDEDGAMIGINKS
                     VSSNMAIMLNDSQVKTISYLSRPEAVLYPEKDLPKEEQFLRDFKWITGKRPYKKDDIF
                     IWERESSEDGNPDVPLRVQPDVLQEEVEF"
     gene            complement(2620..3570)
                     /locus_tag="PKM34_04965"
     CDS             complement(2620..3570)
                     /locus_tag="PKM34_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNQ82972.1"
                     /translation="MKLSREFRIGFLFILATAILVWGFSFLKNKNILYKETTMYAVYK
                     HVNGLNPSDPVYINGVKVGQVDKVHFDMEKKNGDIIVELNFTENFPIQSNSIARIFSE
                     DLMGSKAVEILLGDSPDFAQAGDTLVTEVETSLKDAVNAQIAPLKLKAEELLSSIDTM
                     VVAIQGIFNREAREDLSASIRSIRHTMNNLESTTQSLDTLLITQSSRLSSIIYNIDMI
                     TRNLNNNSQEINRVLDNLASLSDTIARSNISGIISNLDTTVASLSQVMLRIEKGEGTL
                     GMLINDDQLYKDLEKSASELNLLLEDIRLNPKRYVRVSVF"
     gene            complement(3601..4704)
                     /locus_tag="PKM34_04970"
     CDS             complement(3601..4704)
                     /locus_tag="PKM34_04970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ82973.1"
                     /translation="MERLKYIFTTLLFVMACAIPTAAQYQIHRVVIDAGHGGKDPGAL
                     GKNSREKDIALAIALKTGDYIKKNHPDVEVIYTRKTDVFVELYKRAKIANDSKANLFI
                     SIHCNASKSTAASGTETFVMGLHKSDAQLEVAKLENAAILNEENFADMYEGFNPNQDE
                     DYITLTMFQDAFLEQSTMLADEIQRQFRERVKRKDRGVFQAGFLVLYKTTMPGVLVET
                     GFITNPEDERFLMSADGQTYIASAIYRAFKYYKEEMERKDNKAQQVVKEYYKEPDAPS
                     PPPEVWFRVQFTSSKTKKVWDSKKYKDMPDIREYRSDGWYKYSTGNFRTYDDAQRHLK
                     FIRETKKFKDAFLICFLKEERISLEKALELIKK"
     gene            4871..>5773
                     /locus_tag="PKM34_04975"
     CDS             4871..>5773
                     /locus_tag="PKM34_04975"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNQ82974.1"
                     /translation="MPYLVPVLSGIAACILVPVSGFSLPQVEQAPDTVHAISADTLSA
                     DSLRIDSIAPGKSSDALEYKVDYKSADSTFFDLANKKVYMYRNAEINYDDINLKADYI
                     EIDFNTDEIFAKGLPDSTGKDAGLPVFKQGDQQFESRTMRYNYKSKKGLITSVTTEDG
                     SGFLHGETVKKMDDETTNISSGWYTTCSNKDHPHFAFKYGKSKVVSKKRIVTGPAYLV
                     VEEVPTPLAIPFGWFPNKQGQRSGIILPTYGESAQRGFYFEGMGYYLYINDYLDFKVV
                     GDIYTLGSYAIRPSFRYKKRYKYTG"
BASE COUNT         1524 a         1387 c         1420 g         1442 t
ORIGIN      
        1 ccgaaaccga acaatcgatc cgggaccgta ttgccgctaa caaaaagaag atcactgaac
       61 tcaaccagga gattgccggg ctggagaaac aacttggcga tttaaacaac gacgataaca
      121 gttcagagaa catagccgtc gaagttatgg acctcaccct gagctctttc agccattata
      181 tccaggtgag cggaacagcc gaagcggtca ggcaggctta tatttctcct gaagtcagtg
      241 gtcagatcag ggaagtttat gtcaaagaag gcgattatgt cgaaaaaggt aaattattgg
      301 ccaagctgaa ctcggaggtg accgaaagct ccatcgccga tcttcagact tcgctcgacc
      361 tggccggcgt cgtttttgaa aagcagaagc ggctctggga gaaaggaatc ggttccgaaa
      421 ttcagttcct gaacgctaaa aacaacaaag aatcgctcga acaaaagatg cagacgctca
      481 gggcccagct cgatatggcc ctgatcaagt cgccggtaag cggcatcgtc gataagatct
      541 acctgaaaaa gggtgagctg gccatgcccg gcgtgcagtt gatgcaggtg gtcaacctcg
      601 atgagcttta tatcaatgcc gatgtgtcgg aaacttatct cagcgatgtc agggaaggtg
      661 acgatgtgaa ggtggaattc cccgttttcc ccgataaaac ccttgaaact cccatttacc
      721 ggaaaggaaa cgtcatcaac ccaaacaaca ggacctttca aatccagttg aagcttccaa
      781 atccggataa aatgctgaaa ccgaatatcc tctccgtcat ccatatcaat gatttttttg
      841 ccgattcggc tatcgtcgtg ccatcttcca ttataaaaca ggatatttcg ggatcatatc
      901 tttacgcgat catggaaaca ggcaataaat ggacggcgaa gaaagtttat ataaaaaccg
      961 gaaagtctta cctcgacaaa acaatggtga ccgacgggct gaaggaaggg atgaaggtga
     1021 tcaccatggg atataacctg gtgtcggatg gaactgaagt taccgtaaaa caagagaatg
     1081 catcttaatg cggcccacat cagcagaaat ttatttaccg ctctgatcat aataacagcg
     1141 gctttagccc ctgttttccc tttgttcgcc cagaaatcga ccatcgttca catcgaaaat
     1201 gctgacctgc ttcgcgtagc caagatcaat ggaatgagcg taaaaacgtt gaaaggtaat
     1261 gcgatactca ggcaggacag cacctacttc tactgcgata ccgcctcgct ggatgaaaat
     1321 aacaacctga atgccatggg gcgcgtacat attaactatg gcgattcggt ccatctgtat
     1381 ggcgactacc ttacctattc gggcaatacc cggatcgcaa tcatggactc caatgtcagg
     1441 atggtcgaca aaagggcaac cctgtatacc gatcacctgg aatacgaccg aaacctggct
     1501 gtagcctact attttaccgg cggaaagatt gtcgacgacg acaatgtgct gaccagcatc
     1561 cgcggaaaat accttacacg tctcaatgaa tttgagttca gcgattcggt ggtggctacc
     1621 catcccgatt atacgatgta ttcggatacc ctccgttaca ataccgaaac ggagattgtt
     1681 tatatcgaag gcccgaccaa tatttatggt aaagaggatc atatttattc ggagaaagga
     1741 tggtatgata cccgcagtga cctggccgag ctaaaccaga acaaccgcat caggcacctg
     1801 gaacagatcc tgaaaggcga ctggatctac tatgaccggg agaatgaata cggcaaagcc
     1861 aggggccatg tctgggtcat ggatacagtg caggatataa tcatggaagg cggcatcggt
     1921 gaatttcacc gcagcgaaaa gtattcttat atcaccgacc gggcccgcgc catcctgatc
     1981 gatgggagtg attcgctgtt catgcatgcc gacagcttta tcatggtgat ggattcggcc
     2041 gatgaggcaa ggtacctgtt tgcttaccac cggatgaagt tttacaaaca ggacctgcag
     2101 ggcttgtgcg actcgctggt ctacagggtg caggattcgg tcattgccct tttgaagaac
     2161 ccggtactgt ggtctgacga taaccagctc accgccgatt cgatgtggat gcacatcagc
     2221 gataaccgca tcgattcgat ggtactgttc aacatggctt ttatcgtgtc gcgcgacagc
     2281 gtcgaaacct ataaccagat aaaaggcaag cagatgagag cctatttcag ggataatgag
     2341 ctataccgga tcaaagtcct gggcaatgcc gaaacgatct attacgtcag ggatgaagac
     2401 ggagccatga tcgggatcaa taaatcggtt tcaagtaata tggcaataat gctcaacgac
     2461 agccaggtga agaccatcag ttacctttcc cgtcctgaag cggtcctgta tccggaaaaa
     2521 gaccttccga aagaagaaca gtttctgcgt gatttcaaat ggattacggg caaaaggccc
     2581 tataaaaaag atgatatctt tatctgggaa agagagagct cagaagacgg aaaccctgac
     2641 gtaccgcttc gggttcagcc ggatgtcctc caggaggagg ttgagttctg aagccgattt
     2701 ctcaaggtcc ttgtaaagct ggtcgtcgtt gatcagcatc cccagcgtcc cttcgccttt
     2761 ttcaatccga agcatcacct gcgacaggct ggccactgta gtatccagat tggaaatgat
     2821 cccggaaatg ttggagcggg cgattgtgtc gctcagtgat gccaggttgt cgagcacgcg
     2881 gttgatctcc tggctgttgt tgttgagatt gcgcgtgatc atgtcgatgt tgtagatgat
     2941 gctcgataac cggctggatt gggtgatcag aagcgtatcc aggctttgtg tcgtgctttc
     3001 caggttattc atcgtatgcc ggatgctgcg gatgctggcc gacaggtctt cgcgcgcctc
     3061 gcggttgaaa atgccctgga tagccaccac catcgtatcg atcgacgaaa ggagctcctc
     3121 cgctttcagc ttgagcggtg caatctgtgc gttcacggca tctttcagcg atgtttcgac
     3181 ttcagtaacc agggtatcgc cggcctgcgc aaagtcagga ctgtcgccca gcaggatctc
     3241 gacggctttc gaacccatca ggtcttctga aaatatcctg gcaatcgaat tcgactggat
     3301 cgggaaattt tcggtgaaat taagttcgac aatgatatcg ccatttttct tttccatatc
     3361 aaaatggacc ttatccacct gcccgacctt aacgccattg atataaacgg gatcggacgg
     3421 gttcagcccg ttcacatgct tgtaaacggc atacatcgtg gtctccttgt aaaggatgtt
     3481 tttgtttttt aaaaaactaa aaccccacac cagtatcgct gtggcaagaa taaagagaaa
     3541 accgatcctg aattcccggc taagtttcaa aatggtcgtt tttgatgaaa acaaatataa
     3601 ttactttttg atcaattcaa gggctttttc caaagaaatc cgctcctctt tcaggaaaca
     3661 gatgagaaag gcgtctttaa attttttcgt ctcccggata aatttaagat gccgctgggc
     3721 atcgtcataa gtcctgaaat tgccggtaga atacttatac cagccgtcgg acctgtactc
     3781 ccggatatcc ggcatatctt tatatttttt gctgtcccag acttttttgg ttttggaaga
     3841 tgtgaactgc accctgaacc aaacttccgg cggcggggaa ggggcgtcgg gctctttgta
     3901 atactctttc actacctgtt gcgccttgtt gtctttccgc tccatctcct ccttgtaata
     3961 cttgaaagcc cggtaaatgg ccgaagcaat atatgtctgg ccgtcggctg acatcaggaa
     4021 ccgttcatcc tcgggatttg tgataaaacc tgtttcaacc agcacgccgg gcatcgtcgt
     4081 tttataaagg accaggaaac cggcctggaa aaccccgcgg tctttccgct ttaccctttc
     4141 gcggaattgc cgctggatct catcggccag catcgtgctc tgctccagga aagcgtcctg
     4201 gaacatggtc agggtgatgt aatcctcgtc ctggttggga ttaaacccct cgtacatgtc
     4261 ggcgaagttc tcttcattga ggatagcggc attctccagt ttggccacct cgagttgggc
     4321 atcgctcttg tgaagcccca tcacaaacgt ctccgtgccg ctggccgccg tgcttttcga
     4381 agcattgcag tggatcgaga tgaacaggtt cgccttgctg tcgttggcaa tcttcgcccg
     4441 tttgtaaagc tcgacaaata catcggtctt gcgggtgtag atcacctcca catcgggatg
     4501 gttctttttg atgtagtcac ctgtttttaa ggcaatggcc agggcaatgt ctttctccct
     4561 cgagtttttc cccagcgcac cggggtcttt gcccccatgc ccggcgtcga tcaccacccg
     4621 gtggatctgg tattgcgctg cagtcggaat ggcacatgcc atcacgaaaa gcagagtagt
     4681 aaaaatatat ttcaagcgtt ccaccttttt gggaagtttg aatatctttg agttgaaaaa
     4741 cggataaaac ctgcaataat tgcaggaaaa accgttataa tacaaattta gagggataat
     4801 ttccgggatt tttactttgc tgataaaatt attaacagcg tttgttttaa aacggcgacg
     4861 aagttcggcg atgccgtacc tggtgccggt cctgtcaggg atcgcggcct gcatcctggt
     4921 gccggtttcc ggattttcgc tgccccaggt cgaacaggca ccggatacag ttcatgctat
     4981 ttctgccgat accttatccg ctgattccct gcgtatcgat tccattgctc ccggaaaatc
     5041 gtccgatgcc ctggaataca aggtcgacta taaatcggcc gattctacct tcttcgacct
     5101 ggccaataaa aaggtttata tgtatcggaa tgcggagatc aattacgatg atatcaacct
     5161 gaaagccgat tacatcgaaa tagatttcaa taccgatgag atttttgcca aagggcttcc
     5221 cgattctacc ggaaaagatg ccgggctgcc ggtcttcaaa cagggcgatc agcaattcga
     5281 atccagaacg atgcggtata attacaaatc gaagaaaggc ctgatcacca gtgtgactac
     5341 ggaagacgga agcgggttcc tccatggcga aacggtgaaa aagatggatg acgagaccac
     5401 caacatctcc tcggggtggt acacgacatg cagcaacaaa gatcatcctc atttcgcctt
     5461 taaatacgga aaatcaaagg tcgtttcgaa gaagaggatc gtgacggggc cggcgtacct
     5521 ggtcgtggag gaggtcccta cgccgctggc tattcctttc ggctggtttc ccaacaagca
     5581 ggggcaacgg tcaggcatca tcctccctac ttacggtgaa tcggcacaac gggggtttta
     5641 tttcgaaggg atgggatatt atctgtatat taatgattat cttgatttta aagttgtcgg
     5701 ggatatctat acactgggta gttatgccat caggcccagc ttccggtata aaaaacggta
     5761 caaatacacc gga
//
LOCUS       DAOQXS010000132         5759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14252_length_5759_cov_3.430224, whole genome shotgun sequence.
ACCESSION   DAOQXS010000132 DAOQXS010000000
VERSION     DAOQXS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_5759_cov_3.430224"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKM34_04980"
     CDS             <1..244
                     /locus_tag="PKM34_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82975.1"
                     /translation="EGKVRNSFSDVNMTFDPKVNINVDAEMQFGELNYPKSASISHQT
                     ENYTTQVYKGKIGSGTTAGQLTIIGKNANARISFSE"
     gene            266..997
                     /locus_tag="PKM34_04985"
     CDS             266..997
                     /locus_tag="PKM34_04985"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNQ82976.1"
                     /translation="MKMQKFVTHFALLIILGLISTTSYGCRNFPEPRGQENPVKEERN
                     IANFTKLDIGGAFEVILSQGDQVKLVVEAEADELKDIKTEVNGSTLKIYTESTWNPRY
                     HEMRIYLTITKLESVDFSGAVEVRSAGTLSFTDLELNVSGATEIELNLTANKLDAEFS
                     GASEIDLTGKATSGRFELSGASDFDAENLEFQDLSVEVSGASDAKVWATGTLNIDASG
                     ASTIRYKGNPRISLDESGASSVKPL"
     gene            complement(984..3038)
                     /gene="ligA"
                     /locus_tag="PKM34_04990"
     CDS             complement(984..3038)
                     /gene="ligA"
                     /locus_tag="PKM34_04990"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNQ82977.1"
                     /translation="MTTAEARSRMEWLAGEINRHNYRYYVLSDPLISDYEFDMLLEEL
                     VHLENLYPELATADSPTKRVGTDLTKEFMQVDHRYPMLSLGNTYSEQEIIEFDGRVRK
                     LLDNEAPEYVCELKYDGVAIGLTYREGKLVQALTRGDGTRGDDVTNNVKTIKSIPLAL
                     WGDFPFEFEIRGEIFMPRSSFDRLNAEREEIGQPLFANPRNAASGSLKMQDPAEVAKR
                     NLDCYLYFMMGDNLPAGNHYQCLQKAKEWGLKISPYIARCFTPDEIFEFIKTWDSGRR
                     ELPFDIDGIVIKVNDFSQQVILGYTAKSPRWAIAYKFKAERVSTPLLSIDYQVGRTGT
                     VTPVANLKPVFLAGTTVKRASLHNADIIKKLDLHEGDHVFVEKGGEIIPKVVGADPAQ
                     RAFFAPAVKFITHCPECNTPLVRQEGEAAWFCPNDTGCPPQIKGRLEHFISRKAMNID
                     SLGEGKVELLYDKNLVSNIADLYDLTFDQLLGLEKTYENEDGLIERKISFREKTVNNI
                     LAGIEQSKSTPFERVVYALGIRFVGETVAKKLARHFGDIDAIMQAGFEELKQVEEIGE
                     KIAGSVVEFFSKSENRAIVERLRQKGIRMQNDQSVVEIAENKLGGKSFVISGVFARHS
                     RDQLKEMIEKYGGRNTASVTGKTDYLLAGEGMGPAKLKKAGDLGVRIISEEEFLEMIT
                     TV"
     gene            complement(3097..3957)
                     /locus_tag="PKM34_04995"
     CDS             complement(3097..3957)
                     /locus_tag="PKM34_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ82978.1"
                     /translation="MNITSDKENIILVPTDFTPVGQNAIDHGVKACEILGFKLVILHV
                     INRETRAVFGKTDTEKAVQQRLAEIAEAVKTKSTIEVEVAAREGSIFDVISEVAKELK
                     VNLMILGTHGKRGLQHLMGSYAMKVITQSPVPVIVVQKRQIGPGGYRKVVFPISLHTE
                     SRQQVFYAIVTQLLFKGEIHIFKQTGNNPEDNTKLRIITSQITEEFDNKHVRYSLTEA
                     DRASNFTGQLLDFAVSESADLIMMITDSDSIHPTFDNSSWSEKLIFNIAQIPVFCINP
                     VFLGQTYYSF"
     gene            complement(4004..4843)
                     /locus_tag="PKM34_05000"
     CDS             complement(4004..4843)
                     /locus_tag="PKM34_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ82979.1"
                     /translation="METAKNPIFLVPTDFSEVCANAAHRAASLAKDFNYKVVLLHVID
                     RNTISELKKEGKGPEWVDEKLKDLSNALNLEYGVKVETISQEGDIFTTIAEVANDIKA
                     SLIFLGTHGKVGMQKITGSFALKVVTSSELPTIVVQKRPFGKGISTIVMPITSDAGPW
                     AKTKWAASIAKEFNSTIHIFHLPGEEMEDVITMITNHFQVNGVKFEIKEAEKATSFTK
                     QVIDYSTARNADMILIMTNPDKSLKSFLLGSYDEDIIFNTSQIPVMCINPRDFNWKKI
                     IPR"
     gene            complement(4907..5497)
                     /locus_tag="PKM34_05005"
     CDS             complement(4907..5497)
                     /locus_tag="PKM34_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ82980.1"
                     /translation="MNYEAAKKYIITRLEKELDPRLTYHSLSHTLDVLESAVRLADME
                     QLPETEKLLLMTACLYHDSGMLITYRGHEDASIMICREKLPEFEYSAEEISAVCRMIN
                     TTKLPQCANKPADKILCDADLDYLGRPDFFMISHKLKYEWDILNIHPTSLFEWYQIQK
                     DFLSGHRYFTKSAKELRQEFKMQILSQIEFMCNHHK"
     gene            complement(5545..>5759)
                     /locus_tag="PKM34_05010"
     CDS             complement(5545..>5759)
                     /locus_tag="PKM34_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82981.1"
                     /translation="LKLMTKDDYNKALQSYKKKEFEKALNLLQNIEAVNPDDKAVKIY
                     IDRCKHYLKNGVPDMWDGVASFEMKY"
BASE COUNT         1419 a         1350 c         1324 g         1666 t
ORIGIN      
        1 agaaggcaaa gtcaggaact cattctctga cgtcaatatg acctttgatc caaaggtaaa
       61 tatcaatgta gatgccgaga tgcagttcgg tgagctgaat tatccaaaat cggcttccat
      121 cagccatcag accgagaatt atacaacaca ggtttataaa ggtaagatag gcagcggaac
      181 aacggccggc cagctcacca taatagggaa aaatgcgaac gcaagaataa gtttttcgga
      241 ataaatcatt ttaattcaat aaattatgaa aatgcaaaaa tttgtaaccc atttcgcgtt
      301 attgattatc ctcggattga tatcaactac atcatacgga tgccggaatt ttccggaacc
      361 acggggtcag gaaaatcctg ttaaagaaga acgcaacatc gcgaacttta cgaaactgga
      421 tatcggcgga gcttttgaag tcattctatc tcaaggtgac caggtaaagc ttgttgtaga
      481 agccgaggcg gatgaactga aagatatcaa gactgaggtg aatggcagca cgctgaagat
      541 ctataccgaa tcaacctgga atccacgtta tcatgaaatg aggatttacc tgacgattac
      601 gaagctcgaa tctgttgact tcagtggggc ggtagaagtg agaagcgcgg gcacactgag
      661 ttttacggac cttgagctga atgtgagcgg cgccacggag atcgaactaa acctgacagc
      721 caataagttg gatgccgaat tcagtggcgc ctccgagatc gatctgaccg gtaaagccac
      781 ttccggaagg tttgagctgt cgggagcttc cgattttgat gcagagaatc ttgagttcca
      841 ggatctttcg gtagaagtaa gcggtgcatc ggacgccaaa gtgtgggcta caggaacact
      901 aaatattgat gcttccggcg catctacgat acgttacaaa ggaaatccca ggatttctct
      961 cgacgaatcg ggggcttcct ctgttaaacc gttgtgatca tctccaggaa ttcctcttca
     1021 gagatgatcc ttacgcccag gtcgccggct tttttaagct tcgccggccc catgccttca
     1081 cctgccagca aataatctgt tttccccgtt acggaggctg tattgcggcc tccgtatttt
     1141 tcaatcattt ccttgagctg atcgcgcgaa tgacgggcaa aaacgccgct gatcacaaat
     1201 gactttccgc cgagcttgtt ttccgcgatt tctacaacgc tttggtcgtt ctgcatccgg
     1261 atccctttct gcctcaatct ttcaacaata gcgcggttct ccgatttgga aaaaaattca
     1321 acaaccgaac cggcaatctt ttctccgatt tcttccactt gtttcaattc ttcgaaaccg
     1381 gcctgcatga tggcatcgat atcgccgaaa tgccgggcaa gttttttagc caccgtttcg
     1441 ccgacgaacc ggatgcccaa agcatagacg actctttcga agggtgtgga tttcgattgt
     1501 tctatacccg cgaggatatt gttcaccgtc ttttcccgga aactgatctt cctttcaatc
     1561 agaccatctt cattttcata cgttttttcc agtcccagca gttggtcgaa ggtgagatcg
     1621 tacaaatcgg caatattgct gaccaggttt ttatcataga gcaattcaac ttttccttct
     1681 cccaggctgt cgatattcat tgccttgcgg ctgatgaaat gctccaggcg ccctttgatc
     1741 tgtggcgggc aacccgtatc gttggggcag aaccaggccg cttcaccttc ctgccttacc
     1801 aaaggtgtgt tgcattccgg gcagtgagtg ataaatttca cagccggtgc aaaaaaagcc
     1861 ctttgagccg gatcggcgcc cactactttc gggatgatct cgccgccctt ttccacaaaa
     1921 acatgatcac cctcatggag gtcaagtttc ttaatgatgt cggcattgtg gagcgatgcc
     1981 cgttttacgg tagtcccggc caggaacacc ggtttcaggt tagccaccgg tgtcacggtg
     2041 cccgtgcgtc ctacctggta atcgatcgac aacaggggcg tcgaaaccct ttcggctttg
     2101 aacttatagg caattgccca gcggggcgat ttggcagtgt agcccaggat cacttgttga
     2161 gagaaatcgt tgactttaat cacaattcca tcgatatcga aggggagttc cctgcggcct
     2221 gaatcccagg ttttgataaa ttcaaagatt tcgtcaggcg tgaaacaccg ggctatgtag
     2281 ggcgatattt tcagccccca ttccttcgct ttttgaaggc actggtaatg attgccggcc
     2341 gggagattat cccccatcat gaaataaagg tagcagtcga gatttctttt ggccacctcg
     2401 gccggatcct gcattttaag gcttccggat gccgcattcc ggggattggc aaacaacggc
     2461 tggccaattt cttcacgctc tgcattcaat ctgtcgaagc tgctccgggg catgaagatt
     2521 tcgcctctta tttcaaattc gaaggggaag tcgccccaga gagccagggg gatgctttta
     2581 atcgttttaa cattgttcgt cacatcatcg ccccgggtcc cgtcgccccg ggtcagcgcc
     2641 tgcaccagtt tcccttcccg gtatgtaagt cctatggcaa cgccgtcgta cttcagttcg
     2701 cagacatatt cgggcgcttc attatccagc aatttcctga cccttccgtc gaactcaatg
     2761 atttcctgct ctgaatatgt gttaccgagt gagagcatcg ggtacctgtg atccacctgc
     2821 ataaattcct tcgtcagatc ggtcccgacc cgttttgtcg gggagtcggc tgttgccagt
     2881 tcaggataca ggttttccag atgaaccagt tcctccagta acatatcgaa ttcgtaatca
     2941 gaaatcaacg gatcagagag aacgtaatac cggtagttat gccggttgat ctctccggcc
     3001 agccattcca tccggcttct ggcttctgct gttgtcataa catcaagacc ctttatacaa
     3061 aggtcggatt ttttcggatg tcaggatatt gtcttttcag aatgaataat aagtctgccc
     3121 cagaaatacc gggttgatgc aaaatacagg gatttgggcg atattaaaga tcagtttttc
     3181 actccacgaa ctattatcga aagtagggtg gatgctgtcg ctgtcggtga tcatcatgat
     3241 caggtcggca ctttccgata cggcaaaatc gagcaactga ccggtgaagt ttgatgccct
     3301 gtcggcttcg gtcaggctgt atctaacgtg cttattgtca aattcttcgg taatttgtga
     3361 tgtgatgatc cgcagtttgg tattatcctc ggggttattg ccggtctgtt taaagatgtg
     3421 gatctcgcct ttaaacagca attgagtgac aatggcgtaa aacacctgct gacgtgattc
     3481 tgtatggagg cttatcggga aaacgacttt cctgtaaccg ccagggccga tctgcctttt
     3541 ctggactaca atgaccggaa caggcgactg ggtaatgacc ttcatagcat agcttcccat
     3601 tagatgctga aggcctcttt tgccatgggt gcccaggatc atcaggttga cttttaactc
     3661 tttggcgact tcgctgatca catcgaatat acttccttcg cgtgcggcca cctcaacctc
     3721 aatggtcgat ttcgtcttaa ctgcttccgc aatctcagca agtctctgtt gaacagcttt
     3781 ttctgtatcg gtcttgccaa agaccgcccg ggtttctcta ttgataacgt gaaggataac
     3841 cagtttgaat ccgagtattt cacaagcctt taccccgtga tctatggcat tctggcccac
     3901 cggtgtgaag tcggtgggaa ccaggataat attctccttg tccgatgtta tattcatgat
     3961 aagccggagt tatttggtta aaagtgtaag aaagggcccg tatttaccgg gggataattt
     4021 ttttccagtt gaaatcacgc ggattgatgc acatgacggg aatctgtgat gtattgaaga
     4081 tgatatcttc gtcgtagcta cccaggagga acgacttcag gctcttatca ggatttgtca
     4141 tgatcaggat catatccgca tttcttgcag tggaataatc aatgacctgt ttggtgaaac
     4201 tggttgcttt ttccgcttct tttatttcaa atttcacccc gttaacctgg aaatgattgg
     4261 ttatcatggt aatgacgtct tccatctctt cccccggaag gtggaaaata tggatagtcg
     4321 agttgaattc tttggcaatg gatgccgccc attttgtttt ggcccaggga cctgcgtcag
     4381 aagtgatcgg cataacgata gtgctgatcc ctttaccaaa cggccttttc tgaacaacaa
     4441 tggtgggtaa ttcggagctg gtaacgactt tcagcgcaaa actgcccgta attttctgca
     4501 ttcccacttt tccgtgggtt cccaggaata tcaggctggc tttgatgtcg ttggccactt
     4561 ctgcaatcgt tgtaaatata tcgccttcct gtgaaatggt ctcaacctta acaccatatt
     4621 caagatttaa cgcgttcgac aggtctttca gcttttcatc cacccattca ggacctttac
     4681 cctctttctt cagttcgctg atggtgttcc tgtcaatcac atgcagcagg acgactttat
     4741 aattgaagtc tttggcaaga gaagctgcac ggtgggcagc attggcgcac acttccgaaa
     4801 aatcggttgg tacaagaaaa atgggatttt ttgcggtttc catgaacagt tgattttttt
     4861 ttgattaaag caaaaataga aaattatcca ctcagcacga ttatgtttat ttatgatgat
     4921 tgcacataaa ttcgatttga ctcaggatct gcattttgaa ttcctggcgc aactctttcg
     4981 ctgattttgt aaaatatctg tggcctgaca gaaaatcttt ctggatctgg taccattcaa
     5041 acaaactagt cggatgaatg ttcaaaatat cccattcata ttttaacttg tgggagatca
     5101 taaaaaaatc gggccggcct aaataatcca gatcagcatc gcacagaatt ttatcggcgg
     5161 gtttattggc gcattgcggc agtttggtcg tattgatcat cctgcatacg gctgagattt
     5221 cttccgctga atattcgaac tcaggcagtt tctcccggca gatcatgata ctggcgtctt
     5281 catgtccgcg atatgttatc aacatccccg aatcgtgata aaggcaggca gtcatcagca
     5341 agagcttttc cgtctccggc aattgttcca tatctgccag tctgacggct gattcgagaa
     5401 catctagcgt atgggaaagg gaatggtagg tgagccgggg gtccagttct ttttctaatc
     5461 tcgtgatgat atattttttg gcggcttcgt aattcatctt aaaacctttc tgtttaaccg
     5521 gataaccagc ccggaaaatg tttatcaata tttcatttca aaacttgcaa ctccatccca
     5581 catgtcagga acaccatttt ttaaataatg tttacaacgg tcgatatata ttttaacggc
     5641 tttatcgtca ggatttaccg cttctatgtt ttgaagcagg ttgagcgctt tctcaaattc
     5701 ctttttcttg taggattgca gcgccttatt gtaatcgtct ttggtcatga gcttcaggt
//
LOCUS       DAOQXS010000133         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14270_length_5754_cov_3.846815, whole genome shotgun sequence.
ACCESSION   DAOQXS010000133 DAOQXS010000000
VERSION     DAOQXS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14270_length_5754_cov_3.846815"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="PKM34_05015"
     CDS             <1..1477
                     /locus_tag="PKM34_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNQ82982.1"
                     /translation="METITKIIEFSNQVSILEISYILASVLFILGLKKLSHPATARAG
                     NLWAAAGMAAAIVFTILFHKTSVELPDGTFETRGIHNIGWIVAALAIGSFIGWYASVK
                     VKMTAMPQMVSIFNGMGGACAALISMMEFPNMQNTLAYEGSAHMLSGEGISIMLGLMI
                     GSVSFAGSMIAFGKLDGRIGDIKHPVLRFVNLTFLVVLLVALVLILFMKPEAGNNWPI
                     YLFAAAALAYGVTFVMPIGGADMPVVISLLNSFTGVAAAMGGFLYNNTAMLTGGILVG
                     SSGTILTILMCRAMNRSLMNVIIGAFGVAGSKGTESADKTVKEITLSDAAVLLSYSKL
                     VYIVPGYGLAVAQAQHLCNELDHLLSGKGVEVKYAIHPVAGRMPGHMNVLLAEADVPY
                     EKLMEMEDANKEMGNTDVVMVIGANDVVNPAAEDDPSSPIYGMPIIKVLDAKNIIVMK
                     RSMAAGYAAIPNNLFYHRKNSMLFGDAKATLQKLVSEIKSL"
     gene            complement(1511..2278)
                     /locus_tag="PKM34_05020"
     CDS             complement(1511..2278)
                     /locus_tag="PKM34_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ82983.1"
                     /translation="MRIRILLTFFIVSYSAVWAQPLLLLDRFNENKVVNDSTIYVFSS
                     DFNIHDLTQYFTMKNTTDETLAVYLRKSINFIADSTTDYYCFGIRCWPGDDSTNVADS
                     IPPGGEDYTFASHVTHKRRFDLPQPLLPPGYSSITYTVYDKTTLPEPVEASVTVVYHL
                     GGVGFDDTATKRWGDRENVAVYPNPAKDFVNIDLPRPDGSGNIKIYNNLGCEILDFPY
                     REGGLKRFDLSGYPQGIYFLSITGNGRTSTLKFSIVH"
     gene            complement(2393..3142)
                     /locus_tag="PKM34_05025"
     CDS             complement(2393..3142)
                     /locus_tag="PKM34_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82984.1"
                     /translation="MKLILTILCSAVLISTSAQFAPSRLSYKDASQGTSQTGNRDVWQ
                     ACPGNSIYSQIPTLPAQFWTFYSSDLFQGFTPSYLAADQFYGLTSPIGAVHFWGINAF
                     YNYGWYYCNGEDPMTFEITFYEYALDMTGDEVYSEIISISHDYTGNDYSGYQGYHYKA
                     VLSTPVSLTNGWISIQGISDASPENCLFVWQNSYGGNGHALQNSEVIYEDLAFCLEPA
                     VKTVIPLSPWAMALAIFLIIGMVFYRFIRLR"
     gene            complement(3274..5706)
                     /locus_tag="PKM34_05030"
     CDS             complement(3274..5706)
                     /locus_tag="PKM34_05030"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNQ82985.1"
                     /translation="MQLFWKYTFLIFFVFGITGELFSQSPEKKELKALRIAEKPKIDG
                     ILNDSCWQQAAVATGFVQHEPLNGAEPTYQSVFKVAYNNNSIFIGALLYDPEPEKIMR
                     ELGPRDSDDLNADAILVSFSPYNDGQNSFDFALYASGVQVDAKTFSGGDDISWDAVWK
                     SEARITEQGWVAEVEIPFSALRFPRTDAQTWGFNFVRQIRRIREISHWNFVDKEIDGV
                     LNQAGILRGIENVTPPLRLSLEPYLLTSLLKEPGADSWDYKFNGGMDLKYGINESFTL
                     DMTLIPDFSQVQSDDQVVNLGPFETYYSEKRQFFTEGVELFSRGDVFYSRRIGSQPRG
                     YEIIDTSFAAADIISNPEQTRLINATKVSGRTSKGTGIGIFNAMTAPSYAEVKSADGK
                     TPEIQTQPFTNYSMIVADQSLPNKSYISLYNTNVYMGKNGLTANVTGTEMNFRNRKNW
                     LSASAMANVSQKYFPDDSTALGFTYEWSLEKISGKANYGVWQEVISDTYDPNDLGYLE
                     NNNEMEHGGYFSYNIYDPFWKILDMHNSISLEFKHLYAPGVFTGMEMNIENRTQFKNR
                     LTVGLEAEISPAETRDYFEPRNAGWFVQYPASASISTFFSPDYNKTFLVDVMPGISWA
                     SDYGQLGYYLRLSPRYKVNPHLFLVLTALYSRSLNDIGYVTDSLENAGLKIIFGRRDI
                     ENITTTLTVNYSVNPKFSFSGRLRYYYFKADYKGYYDLGKDGSLSPNGYTGADDFLYS
                     AFNIDAYFTWLFAPGSELVLAWKNAIYTNKDLPAGNYFREFGKTLDSPASNLISLKIL
                     YYLDSQYFRKKH"
BASE COUNT         1452 a         1392 c         1466 g         1444 t
ORIGIN      
        1 catggaaaca atcacgaaaa tcatcgaatt ctcgaaccag gtatccattc ttgaaatttc
       61 ttacatcctg gcatcggtcc tttttattct cggattgaaa aaactcagtc accccgccac
      121 ggcacgcgcc gggaacctgt gggctgcagc gggtatggca gccgccatcg tctttaccat
      181 tcttttccat aaaaccagcg tggagcttcc tgacggcacc tttgaaaccc ggggaatcca
      241 caacatcggc tggatcgttg ccgcgctggc catcggctcg tttatcggct ggtacgcctc
      301 ggtgaaggtg aaaatgaccg ccatgccgca gatggtctcc atattcaacg ggatgggagg
      361 cgcctgtgcc gccctgatct ccatgatgga gttcccgaat atgcagaata cgctggccta
      421 cgaagggtca gcccatatgc tcagcgggga ggggatctcc atcatgctgg ggctgatgat
      481 cgggagcgtt tcctttgccg gcagcatgat tgccttcgga aagctcgacg ggcgcatcgg
      541 ggatatcaag cacccggtgc ttcgttttgt caacctgaca tttctcgtgg tcctgctggt
      601 agcgctggta cttattctct ttatgaaacc ggaagccgga aacaactggc ccatatacct
      661 gttcgcagcg gcagcgcttg catacggcgt gaccttcgtg atgccgatcg gcggtgccga
      721 tatgcccgtc gtcatctcgc ttttgaactc attcaccggc gtcgcggctg ccatgggcgg
      781 tttcctgtat aacaacacag ccatgctaac gggcggtata ctcgtcggat catcgggtac
      841 aatcctgacc atcttaatgt gcagggcgat gaaccgctcg ctgatgaatg tcatcattgg
      901 cgcattcggg gttgccggtt ccaagggcac cgagtcggcc gacaagactg taaaagagat
      961 cacgctcagc gatgccgccg ttctgctgag ctattccaag ctggtttaca tcgtccccgg
     1021 ttacgggttg gcggtagccc aggcacagca tctttgcaat gaactcgacc acctgctttc
     1081 cggcaaaggg gttgaggtga aatacgcgat ccatccggtg gcaggccgaa tgcccggcca
     1141 tatgaatgta ttgctggctg aagccgatgt cccttatgaa aagctcatgg agatggaaga
     1201 tgccaacaag gagatgggca acaccgacgt ggtgatggtg attggagcca acgatgtggt
     1261 aaaccctgcc gcagaggacg atccttcaag cccgatctac ggcatgccca tcattaaagt
     1321 gctggacgcc aagaatatca ttgttatgaa gcgcagtatg gcagccggtt atgccgccat
     1381 ccccaacaac ctgttttacc accggaagaa cagtatgctg tttggcgacg ctaaggccac
     1441 cctgcagaaa cttgtatcgg aaatcaagag cctgtaaaaa cttctgtaaa gcgtaaagat
     1501 ttaaacctca tcaatgcacg atgctgaatt tcagtgtcga cgttcttcca ttacctgtta
     1561 tggagagaaa atagataccc tggggatagc cactcagatc gaaacgtttt aaaccgcctt
     1621 cccggtaagg gaagtcgaga atctcacaac ccaggttgtt gtatattttt atgtttccgc
     1681 tgccgtctgg ccggggcaaa tcaatattga caaaatcttt agccggattc ggatatactg
     1741 ccacattctc cctgtctccc catctcttcg tcgccgtgtc atcaaaccct actccgccaa
     1801 ggtgatacac tactgtcacc gacgcttcaa ccggttccgg gagtgttgtt ttatcgtaaa
     1861 cggtataagt gatggaagaa taccccggcg ggagcaaagg ttgcggcagg tcgaaccggc
     1921 gtttatgggt gacatgtgaa gcaaacgtat aatcttcgcc gccgggcggg atgctgtcgg
     1981 ctacattggt ggaatcgtca cccggccagc agcggatacc gaaacagtag tagtcggtag
     2041 tactgtcggc gataaaattg atgctcttcc ggagatagac tgccagcgtt tcatccgtgg
     2101 tgttcttcat cgtgaagtac tgcgtcagat cgtggatatt gaaatcgctg ctgaagacat
     2161 atatcgtcga atcgttcacc actttgttct cgttgaaccg gtcgagcagc agcaaaggct
     2221 gggcccaaac ggcggaatat gaaacaataa aaaaagtaag cagtattctt atcctcatat
     2281 ataatatttt aaatacaaag gtatgaaaat caaatggagg gatcaataaa aaataatcat
     2341 cccgggaaga aaatcatcct tgaagaaaat tccgaagaat acttaaaagt acttaccgta
     2401 atcgtataaa ccggtaaaaa accatgccga tgatcaggaa aatagccaat gccatggccc
     2461 agggcgacaa tgggatcacc gtcttaacgg caggctcgag gcaaaaagcg aggtcctcgt
     2521 aaataacctc actgttttgc aaagcgtggc cattgccgcc atacgagttc tgccacacga
     2581 ataaacagtt ctccggggag gcatcactta taccctggat agagatccaa ccattagtca
     2641 gactgaccgg agtacttaaa acggctttgt aatgataacc ttgatatcct gaataatcat
     2701 ttccggtata atcatggctg atggatataa tttcggaata tacttcatca ccagtcatat
     2761 caagggcgta ttcatagaaa gttatttcaa atgtcatagg atcttcgcca ttacaataat
     2821 accagccata attataaaat gcatttattc cccaaaagtg aactgcacct attggcgatg
     2881 tcaatccgta gaattgatct gctgcaaggt acgaaggtgt aaatccttga aataagtcgc
     2941 tggaataaaa tgtccagaat tgtgccggca gggtagggat ctgggaatag attgaattgc
     3001 cgggacaggc ttgccagacg tcgcggttac cggtttggga cgtgccttgt gaagcatctt
     3061 tgtagctcag gcgggaaggc gcaaactggg cactggtgct gatcagaaca gcactacaca
     3121 gaatggttaa aataagtttc atggttttga gaagtttggt ttgttatttc ccgtaaagtt
     3181 atcagatgaa tctcataaag tcaagaatta catgtacgaa taggcgcctt tgaagcttag
     3241 atgtacatct ttttaatatt ttgaggcttt ttcttaatgt tttttcctga aatactgcga
     3301 atcaaggtaa tagaggatct tcagggagat gaggttggat gccggcgaat caagcgtttt
     3361 gccaaactcc cggaagtaat tgccggccgg caggtcttta ttcgtgtaaa tagcgttttt
     3421 ccaggccagg accagctcgc tgccgggggc aaacaaccag gtaaaatagg catcgatatt
     3481 gaaagcgctg taaaggaaat catccgcacc cgtgtaacca tttggcgaga ggctcccgtc
     3541 tttgcccagg tcatagtacc ccttgtaatc tgctttaaaa taataatacc gcagccgccc
     3601 gctaaaactg aatttcggat tgaccgaata attgaccgtt aatgtagtgg tgatattttc
     3661 gatatcccgc cggccgaaaa tgatcttcag tccggcattc tcaagactat cggtaacata
     3721 accgatatcg ttgaggctcc ggctgtaaag ggctgtaaga acgaggaaga gatgcggatt
     3781 gaccttgtaa cggggcgaaa ggcggaggta ataccccagc tggccgtaat ccgacgccca
     3841 ggatatgccc ggcatcacat ccaccaggaa cgttttgttg taatcggggg agaagaatgt
     3901 gctgatggag gctgaggcgg ggtactgtac aaaccatcct gcattgcgcg gttcgaaata
     3961 atcgcgcgtc tccgccgggc tgatctctgc ttccagtccg acagtgagcc ggtttttaaa
     4021 ctgggtcctg ttttcgatat tcatctccat ccccgtaaac acccccggag catataaatg
     4081 cttaaattcc aggctgatgc tgttatgcat gtcgagtatc ttccagaatg ggtcgtagat
     4141 gttgtaactg aaatacccgc catgttccat ctcgttgttg ttttccaggt agccgaggtc
     4201 attcggatca taggtatcgg agattacctc ctgccatacg ccgtaattgg ctttgccgct
     4261 gatcttttcc agcgaccatt cataggtgaa accgagcgcg gttgaatcat ccgggaagta
     4321 cttctgagaa acattggcca ttgccgatgc cgacagccag tttttcctgt tgcggaaatt
     4381 catctccgtg ccggtcacat tggcggttaa gccgtttttt cccatgtaaa cattggtatt
     4441 atacaggctg atgtacgatt tgttcggcag cgactggtcg gccacaatca tgctgtagtt
     4501 ggtgaagggc tgggtctgga tctccggcgt cttaccgtca gcgcttttca cttcagcata
     4561 ggaaggggct gtcattgcat tgaagatgcc gattccggtg cctttcgaag tgcgtcccga
     4621 tactttggtg gcatttatca gccgggtttg ttccggatta ctgatgatat cggcagcggc
     4681 aaaagaggtg tcgatgattt cgtatcccct gggctgtgat ccgatcctgc gggaatagaa
     4741 cacatcgccg cggctgaaca gttcgacccc ttcggtgaag aactgccgct tttcgctgta
     4801 ataggtctcg aaaggcccga ggttgaccac ctggtcgtcc gattgcacct ggctaaaatc
     4861 ggggataagg gtcatgtcga gcgtaaagct ttcgttgata ccgtacttca ggtccatccc
     4921 gccgttgaat ttataatccc aggaatccgc acctggttct ttaagcagtg atgtcagcag
     4981 gtatggttct aaagaaagcc ggagcggagg cgtgacattc tcgatgcccc ggagaatgcc
     5041 ggcctggttg agaaccccgt cgatctcttt atcgacgaag ttccagtgcg agatttcgcg
     5101 gatgcgcctg atctgcctga caaagttaaa tccccaggtc tgcgcgtcag tccggggaaa
     5161 gcgaagtgcc gagaagggta tctccacctc ggccacccat ccctgttcag tgatcctggc
     5221 ttcgcttttc cacaccgcgt cccacgatat atcgtcgcca ccagagaatg tttttgcgtc
     5281 tacctgcaca ccggaagcat ataatgcgaa atcaaaactg ttctgaccgt cgttgtaagg
     5341 cgagaagctg accagaatgg catcggcgtt caggtcgtcg gaatcgcgcg ggcccagctc
     5401 gcgcatgatc ttttccggct ccggatcata cagcaaagcg ccgatgaaaa tcgaattatt
     5461 gttataagct accttaaaaa cagactgata agtcggttcg gcgccattga gcggttcgtg
     5521 ctgaacgaag cccgtggcca cagcggcctg ctgccagcac gaatcgttga gaatgccgtc
     5581 gattttaggc ttttcagcga tgcggagggc tttcagctct ttcttttcag gtgattggga
     5641 aaataattcc ccggtaatgc cgaaaacaaa aaaaatcagg aaagtatatt tccagaataa
     5701 ttgcatgagc cgggatattt taaatcagtc agctggcgaa attaaaagaa attc
//
LOCUS       DAOQXS010000134         5732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14324_length_5732_cov_3.900828, whole genome shotgun sequence.
ACCESSION   DAOQXS010000134 DAOQXS010000000
VERSION     DAOQXS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14324_length_5732_cov_3.900828"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKM34_05035"
     CDS             <1..811
                     /locus_tag="PKM34_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82986.1"
                     /translation="GTSVLKADFSNINNFEQTEEFQPYLGVHIDFNYYFNDGPRRFTK
                     GLSMGAGWSRFNATGKVFDYNASFTEFSEPFDQNYTKYVYADNIHENLMLDYADVNLL
                     IKLRYRLFKNTELIGSIGPQASFLINSKIEPEAGSEGFVNYEGYFTDIEFPNGTSGEF
                     LLQNVPEYGFTKYTDLKFSENEIGINKFNFCAYADIGLNLKMSHKFHLSFGIGYMYGF
                     GNLVSESSGEEFVLSKGEGEVDNLFKNCTSLKISAPSAFVSAAYLINTKIK"
     gene            821..1951
                     /locus_tag="PKM34_05040"
     CDS             821..1951
                     /locus_tag="PKM34_05040"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ82987.1"
                     /translation="MKQFILSLFFLATSVMLFGQEEQCLFRTQPQDTETCLNGLAVFD
                     AGVDTLFFENSFFFEWQYKPAGAADWIPVQNNGTFQVTNTQLSSMLTVTINGDASLDG
                     ADFRCSVSDLYYSESASMVIHSLPVVSFTSQNYCFGDITGFTNTSPDAASMKSWAWEF
                     SDGSKYISRDINHRFLLPGDYKVKLTLTNPSDCEAADSAVVTILPLPEPVIKYTKDVF
                     CGFESNLTFYTEDIFESYLWEIRGANNVLASEETEITFDCDENFPTGQYEAILMVTDA
                     NGCSADVRKSFLVLSSQAPVDGTVLQKEDDSRLLVLLIDHQENSDFLWMKMDPVTKTV
                     LEQYKTKEPYHLFGEPIDKNSFEYGVEVIPPFSDCSAVFYLN"
     gene            2253..4481
                     /locus_tag="PKM34_05045"
     CDS             2253..4481
                     /locus_tag="PKM34_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82988.1"
                     /translation="MIFNDIAQDEDMSLYITPAEYIKKVSELYPIGLYVYISDAEIID
                     VREYSSYILYIVEIEKTIYIDNMLRTNRPVPKFNSPVRQIYYIIGYSNSPGIKIIGIE
                     AKGTAADNKTAYNGLVPDKLEFNAHFNAYNLNFKDIREEYAGLDGSGNQTRFGLLLGW
                     DLGGKKSFRYGLASGLLYDKVNFSTDLESFADRPIPAVDIDNYPYRKIITGHDISQSN
                     SLDQLTLPVLFSMNWEFPPKWGNKYVKRSFTGDLAYKRGIGINFRIGPHIQYNLSGKS
                     DPYEGTFSYGGSYRFFNPVVQDSTTIIIDNLPEYGFVNDTTFTDNSSVASYSKINIGF
                     STQLNVSIPIYKSLEFYFGPSLYYNFSGLAETTEDFILSGRIGEINPFITAAKTSSLS
                     YGVNAGLVLNLHAPRVPYSSIELPDIAKDRYKVKTAYTLENDNRMKMALNVNLIAGKS
                     MEMERQAINYQIQSDWAKKPTKGKFAGFKTHILKYEYPKIAGKVTFKSDLVIEKPFGY
                     DVVCNDTIAFNSVNTNPLVIPLDNLNRLAENRQSLDLTVNRLPDFTFVYVSLYNKFET
                     LEQRRKIVELVRDLGNNAIINQEELLVYLSSEAGKPIAYCNFASREDLPENVVTDWQE
                     FLYKLKEEYGSQNLIYEDDIRNIDNILNPILNLDERMNAQRRFVNFNFIVNDPEKYYG
                     YDSERLSDTRALIHFLSDKYCSKIPFRSSQFDFNVYLTERNLNMRKSPSDININIIHL
                     IK"
     gene            4492..>5732
                     /gene="tssR"
                     /locus_tag="PKM34_05050"
     CDS             4492..>5732
                     /gene="tssR"
                     /locus_tag="PKM34_05050"
                     /inference="COORDINATES: protein motif:HMM:NF036394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssR"
                     /protein_id="HNQ82989.1"
                     /translation="MKKYISQLLILSSFFLSLQMVSGQELIRKPNSELINSVNEIIGK
                     TSYERGTTKWFVYSNRKGNTVYSDAGSVISTNVKFLETFNVVAEDNNRLKVKKLDDGE
                     VVGWMDKDDLIILDHCERKANMVSKKAMIINTIDRASANANRDSSQFRFYFDPDLTKR
                     NPKEQSSVYEILYVYKESGNALLLGRREDCSVDVEAYLKESIIGWIAKESVTEWDSRG
                     CLLPNYKPEAKTERRTQGTQPAILDTSSRYTDYSLAKAYDSVLLTYANLKGYAETSFD
                     RSPIVWNEEIDNRWPPSKFRLPYLDKHNNDIDIMKVGVLGDITDAQGRQIQALIDNLR
                     EWERQNRTKNSLNLVFVIDGTYSMKNYFPAVANAISESHKIISKDPDFSDPDNPNRVT
                     VKCGAVVYRDLAEGEYLVETSP"
BASE COUNT         1696 a         1240 c         1263 g         1533 t
ORIGIN      
        1 aggaaccagt gtcctgaaag ccgatttttc aaacatcaat aattttgagc aaacagagga
       61 gtttcagccc tatctcgggg ttcatatcga tttcaactat tattttaacg atgggccaag
      121 aagatttaca aaaggactga gtatgggtgc gggctggtcg aggtttaacg cgaccggtaa
      181 ggtttttgat tacaatgcct catttacgga attttccgaa ccttttgacc agaactatac
      241 taaatatgta tatgccgata atatccatga aaacctgatg ctggattatg cggatgtcaa
      301 tctgctcatt aaactccggt accggttgtt caagaatacg gaactgatcg gctcaatagg
      361 gcctcaggct tctttcctca tcaatagtaa aatcgaacct gaagcaggca gcgaaggatt
      421 tgtcaattat gaaggatatt ttaccgatat cgaatttccc aatgggacca gcggagaatt
      481 cctccttcag aatgtccctg aatatggttt cacaaaatat actgacctga agttcagtga
      541 gaatgaaatt ggaataaaca aattcaattt ctgtgcctat gctgatatcg gccttaacct
      601 gaagatgagc cataagttcc acctatcttt cggtatcggg tacatgtatg gctttggcaa
      661 cctggtttcc gaatcatccg gggaggaatt cgtcctgtcg aaaggggagg gagaagtgga
      721 caacctcttc aagaattgca catctttgaa aatcagtgcc ccaagcgctt ttgtttccgc
      781 tgcttacctt attaacacta aaattaaata gccctgccat atgaaacaat tcatactatc
      841 cttatttttc ctggccactt cggtgatgct tttcggacag gaagagcaat gccttttcag
      901 gacccagccg caggataccg aaacctgtct gaatggcctg gccgtgttcg acgccggcgt
      961 ggataccctg ttctttgaaa acagcttttt cttcgaatgg cagtacaaac ccgccggagc
     1021 tgccgattgg ataccggtac aaaataacgg cacgttccag gttaccaata cccagcttag
     1081 ttctatgctg acggttacaa taaacggcga cgcctccctt gacggagctg atttccgatg
     1141 ctcagtcagc gatctttact atagtgaatc agcatccatg gtgatccatt cgcttccggt
     1201 cgtttcgttt acctcccaga attattgttt cggagatatt accggattta cgaataccag
     1261 cccggacgcc gcctccatga aaagctgggc ctgggaattc tcagacggct ccaaatacat
     1321 cagccgcgat atcaatcacc gcttcctgct gccgggcgac tataaagtga aactgacact
     1381 gaccaacccg tcggactgcg aagcggcaga cagtgcggta gtgacaatcc tgccacttcc
     1441 cgaacctgta attaagtata caaaggacgt cttttgcgga tttgaaagca acctgacctt
     1501 ctacaccgag gatatctttg aaagttatct gtgggagatc aggggagcaa acaacgtcct
     1561 ggcaagtgaa gagaccgaga taacgttcga ttgcgacgaa aatttcccta caggacagta
     1621 tgaagccata ctaatggtga ccgatgctaa cggttgttcg gcagatgtca ggaagagttt
     1681 cctggtactt agcagccagg cgccggtcga tggaaccgtt ttgcagaaag aggacgatag
     1741 ccgcctgctc gtcctgctga tcgatcacca ggagaattcc gactttctct ggatgaaaat
     1801 ggacccggtc accaaaacgg tcctcgaaca atacaagacg aaagagccat atcatctttt
     1861 cggtgaaccg attgataaaa acagttttga atacggtgtg gaggtcatcc ccccattttc
     1921 cgactgtagc gcagtattct atctaaattg attttgcgcc gttacaatga tatcttaagc
     1981 gtacattaaa gaaattaaat atgatattga tgaaaaaggc gaatatgaaa tcctggaata
     2041 aatggttctc aatttggtgc tttgcaatgt tcttcggatc atctggaata ttgaacgccc
     2101 aaaatgttcc tttcgaggta aggaatttcc tggctgatta tgagagagtt gttgacatca
     2161 gtgaatttga tgattccgac acaatgccta ccaataggtt ttcagctgca gcagcagaaa
     2221 aattcactaa tttttttcta tctaaggaaa cattgatttt taatgacatt gctcaggatg
     2281 aagacatgtc actttatatc actcctgctg aatacattaa gaaagtaagt gagttatatc
     2341 ccataggatt atatgtttat atttccgatg ctgaaattat tgatgtcagg gaatattcat
     2401 cttacatcct ctacatcgtg gaaattgaaa aaaccatata tatcgacaac atgctgcgta
     2461 caaaccggcc tgttccaaag ttcaattctc ccgtcaggca gatatattat attatcggtt
     2521 atagcaattc tccgggcata aagatcatag gaattgaagc aaaaggtacg gccgccgaca
     2581 ataaaacggc ttacaacgga ctggttccgg ataagctgga gtttaatgcc catttcaatg
     2641 cctacaacct gaattttaaa gacatccgcg aggaatatgc aggccttgac ggttcgggca
     2701 atcaaacccg ctttggactg ctgctgggct gggatttggg tggtaaaaag agtttccggt
     2761 atggattggc ctcgggactg ctttatgaca aggtcaattt ttctaccgat ctggaaagtt
     2821 ttgccgatcg gccgattcca gcagttgata tagataacta cccataccgg aaaatcatta
     2881 ccggacatga catcagccag tccaattcac tggaccagct gactctaccg gtattattca
     2941 gtatgaactg ggaatttcct cccaagtggg gaaataagta cgtaaaaaga tcattcacag
     3001 gagacctggc ctataagcgc gggatcggca tcaatttcag gatcgggccg catattcagt
     3061 acaacctgag tggaaagagt gatccttacg aaggcacatt cagttatgga ggaagttacc
     3121 gtttcttcaa tcctgttgta caagacagta caactattat tatcgataac ctgccggaat
     3181 atggttttgt taacgatacg acttttacgg ataatagttc tgtcgcgagt tacagtaaga
     3241 tcaatattgg attctccaca caactgaatg tttccatccc catctacaaa tcactcgagt
     3301 tttactttgg cccaagcctc tattacaatt tttcgggcct ggctgaaaca actgaagatt
     3361 ttatcctgtc aggccggatc ggggagatca atccatttat tactgctgct aaaacaagct
     3421 ctttgtcata tggagtcaat gccgggctgg ttctgaacct ccacgccccg cgtgtgccct
     3481 attcatccat tgagttaccg gatatagcca aggatcgcta caaagtcaaa acggcctata
     3541 ctcttgagaa tgataatagg atgaaaatgg ctttgaacgt taatctgatc gccgggaaaa
     3601 gcatggaaat ggaacgtcaa gcgatcaatt accagataca gtcggattgg gcgaaaaaac
     3661 cgacgaaagg aaaatttgcc ggttttaaga cccatattct aaaatacgag tacccgaaaa
     3721 tagccggcaa agtcaccttt aaaagcgacc tcgtcatcga aaagccattc ggttatgatg
     3781 tggtttgcaa cgatacgatc gcattcaata gcgtaaatac caatccgttg gttattccgc
     3841 tcgataatct gaacaggctt gctgaaaacc gtcaatcact cgatctgacc gtgaaccggc
     3901 tgcctgattt cacatttgtc tatgtttcac tttacaacaa atttgaaaca ttggaacaaa
     3961 gaaggaaaat cgtagaactg gtgcgggatc tgggtaataa tgccatcatc aaccaggaag
     4021 aactgctggt gtacctgagt tctgaggccg gtaagccgat agcttactgc aacttcgcct
     4081 caagagagga tctgcctgaa aatgttgtta ccgactggca ggagtttctt tataaattaa
     4141 aagaagaata tggctctcag aacctgattt atgaagatga tattcgtaac attgataata
     4201 tcctgaaccc tatcctgaat cttgatgaaa ggatgaatgc ccagcgacgt tttgtcaatt
     4261 tcaattttat tgttaatgac ccggagaagt attatggtta cgactcggaa cgtctttctg
     4321 atacaagggc tttaatccat ttcctttccg ataaatattg ttcgaagatt cctttcaggt
     4381 cctcacaatt cgatttcaat gtctatctta ctgaaagaaa tttgaatatg agaaaatctc
     4441 ccagtgatat caatattaat attattcatt taatcaaata atgtttctgc aatgaaaaaa
     4501 tacatatccc aactgctaat tttaagttcc ttcttcttaa gcctgcaaat ggtatccggg
     4561 caggaactga tcagaaaacc aaattccgag ctgatcaaca gtgtgaatga gatcattgga
     4621 aagaccagtt acgagcgcgg aacgacaaaa tggttcgttt attccaacag gaaaggcaat
     4681 actgtttaca gcgatgcggg ttcagtgata agcaccaacg tgaagttcct ggaaactttt
     4741 aatgtggttg cggaagataa caacaggctc aaagttaaaa agctggatga tggcgaggtc
     4801 gtgggctgga tggacaagga tgacctgatc atcctggatc attgtgaacg caaagccaac
     4861 atggtttcca agaaagcaat gatcatcaat acgatcgaca gagctagcgc taatgctaac
     4921 agggacagca gccagttccg gttttatttc gaccctgacc tgacaaagag aaacccaaaa
     4981 gaacaaagca gtgtctatga gatcttatat gtttacaagg aaagtggaaa tgcattactc
     5041 ctgggcagac gcgaagactg ttcggtcgac gttgaagctt atctcaaaga atcgatcatc
     5101 ggatggattg ccaaagaatc ggtaaccgag tgggattccc ggggatgcct tttgcctaat
     5161 tataaacctg aagcaaaaac tgaaagaagg acccagggca cacagccggc tattctcgat
     5221 accagttcaa gatatactga ttattcgctg gccaaggctt atgactccgt cttattgact
     5281 tacgcgaacc taaagggtta tgctgaaact tcctttgaca gatcacctat tgtatggaat
     5341 gaagaaattg ataatcgatg gccaccttcg aaattcaggc tgccttacct tgacaaacat
     5401 aataacgata tcgatatcat gaaggttggt gttctgggtg atattaccga tgcgcagggt
     5461 agacagatcc aggcattaat tgataacctg agggaatggg agcgccagaa caggacgaaa
     5521 aacagcctga acctggtctt cgtcatcgat ggtacctata gcatgaagaa ttattttcca
     5581 gccgttgcca acgccatcag cgaaagccat aagatcatca gcaaagatcc cgacttctcc
     5641 gaccctgata acccgaacag ggttaccgtt aagtgcggcg cagttgttta ccgcgatctt
     5701 gccgaaggcg agtaccttgt ggaaaccagt cc
//
LOCUS       DAOQXS010000135         5693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14443_length_5693_cov_3.951046, whole genome shotgun sequence.
ACCESSION   DAOQXS010000135 DAOQXS010000000
VERSION     DAOQXS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14443_length_5693_cov_3.951046"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..699
                     /locus_tag="PKM34_05055"
     CDS             67..699
                     /locus_tag="PKM34_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82990.1"
                     /translation="MKKITLTSLLTIMCISLALSQNQRKSATENEADHKFSFGTTYLI
                     LANFGQEKTNTHHYEFHGSYQLTPKDRIGIKVATWKLFAPMGIQLWDPGFRDESEFFP
                     GRLSECGIGVTYQRMLWKGLFAAVEVLPLFKIYLDEEKNKIDEGFKLYTTWHLGYHIA
                     LFKNRFYLEPQVHCNYWPVDTKAPQGFEEKESKWKNYFLFEPNLYFGINF"
     gene            740..2179
                     /locus_tag="PKM34_05060"
     CDS             740..2179
                     /locus_tag="PKM34_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNQ82991.1"
                     /translation="MKKLALSSVIILSTLSITFSQVIPTDSLYFGQTPPGCIPQLFAP
                     GIVSIPDRNEATITFSPDGASVFFHIEKYPEPGNPYILSASYLEDHWTTPDTIPFSNG
                     RATGEPFFAYNGNRVYLFATNAVNHEGIADLSFSQKEGDSWSDPVSLGNPPNSESYQY
                     HPCIVGDSSVYFSSQAGKICRSQYCDGIYENRIVLPKPVNFIGSQTWGDPYVSADESY
                     MIMKAIREEGYGQNDLYIAYRKPDGNWTNPKNLGNVINTPGDETSGDITPDGLYMTYG
                     SNDDLYWVSAGFTDSLRYTNFIPYMKYPIPDQTAYIGEQFIYSIPDSTFSDDDSSSVF
                     TFTAVLTNGNPLPEWLTFDTVSGVFTGVPEMEQILNIKLTANDNAGASAWTPFKLSVQ
                     LPTGYDPVNFHALTIFPNPNRGLITITLGPFTGKTATVVLNDLQGRSFVRQVFKNETI
                     IDLTDCSSGVYILKVYVENELISRKIYKM"
     gene            2209..2880
                     /locus_tag="PKM34_05065"
     CDS             2209..2880
                     /locus_tag="PKM34_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82992.1"
                     /translation="MKRFTIVFIAVVSILLANAQTKLDYSISVIYNRSSQTNYNPQLP
                     DDGTFQWNALNTFGIGVNIGKPLSDRVYWTNRLLCQQKGYSQEAQTADFIDGIPGPLS
                     YHKLQDRFGYLTIESNIGYHIIKFKSLKLTPLIGFNINYLLSKSIESEGVYPINSCYP
                     IDEYNGNWKKINVGYNVGISCAIQKKVNLGFEFQRSITPLLKTNDLTVKDWTWSVRLD
                     FLLSD"
     gene            2964..3119
                     /locus_tag="PKM34_05070"
     CDS             2964..3119
                     /locus_tag="PKM34_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82993.1"
                     /translation="MDHKEQIIVYLISKIHELKEKIREDKLSKEELIIYIEMLEDMLR
                     DYFNLSD"
     gene            3193..4560
                     /locus_tag="PKM34_05075"
     CDS             3193..4560
                     /locus_tag="PKM34_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82994.1"
                     /translation="MAKISIQPGFSIIVPYKADDNTGNVRKGRVEDNDIIIVNNFDKK
                     GIKTEQSYYNTSGELIEQVVCKPDDSGKLAEKICLYGIENPDIKPDPHCSNLDSGREG
                     TDSFENYTFFPASDQKVCLTSYKYDKKGRLIEEYRYSDNRKLINKVVITYNDRGEVQE
                     YIQSDHLGYPDFTNKYRYDDDHRLVEVEEFNSVDELACKTVNTYDDQGNLTEKRLYDE
                     RGELSKKIKQEYDDSGNLVLQEEYDRGTLLTKTTRYRYNVKKILFEKILDGFSMGGTY
                     RYLYTLGAGNASTETVVFKNDKAYKMYEIQYGLSAGEVKQKLAALKSLGPVNVLHDLR
                     DEWEENKMFIINRFGKEAYQNFLEPYNIVQIELNRLFSKKSIPTTYDDLKKFIETNQE
                     IRRISDYNAPHQLDHFSRVLKVPKWKLKLGMWAGSDEMDYKEAMRSGRLLIWLNALEF
                     YEARF"
     gene            4585..>5693
                     /locus_tag="PKM34_05080"
     CDS             4585..>5693
                     /locus_tag="PKM34_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82995.1"
                     /translation="MKYLKIPSRPGHLRITVPDQFFHSIILILFLMTVCSCASLDSTN
                     YKSRLRAVKKTDDQSILYKVALGDVNREVKLLALSRITDQELLGKYADEMNDPGLQQM
                     AIGMITDQEILFEIALDHASSDIKMAAFHKISDMKLVNRLAVEANDPKIQLPAIGKTD
                     SQEVLFKAAMDDSRTEVRQAAFDKLTDPALIGKLAIGSGDTKLQLKAIEKVSDQNTLF
                     EIALKEDYKEAKHAAMIRLTDEQLLGKLANESKDTFFRLMVISKITDQDILYKIALED
                     ENRDAQKTALSRLSPEMLTRLVSDYDELEMQLKVIEILDDQTGLMNISQNHTDWQVRR
                     AAFTKLDDVSLDLLSREATDPAIMLGAKIRRGIIS"
BASE COUNT         1745 a         1180 c         1271 g         1497 t
ORIGIN      
        1 agctctaatt tatttccatt cattttgata attgtaagtt ttctgaaaat cataaagatc
       61 tgaataatga aaaaaatcac tttaacatcg ctattgacga tcatgtgcat atcgcttgca
      121 ctgagtcaga atcaaaggaa atcagccaca gaaaatgaag ctgaccacaa attttccttc
      181 ggaacaacct acctgatcct ggccaatttc ggccaggaaa aaaccaacac gcatcattac
      241 gagtttcacg gcagctatca actcacgcca aaagatagga tcggcattaa agtagccaca
      301 tggaagcttt ttgccccgat gggcatacag ttatgggatc ccggattccg tgacgaaagc
      361 gagttctttc cgggcaggct cagcgaatgc ggcattgggg ttacctacca gcggatgctg
      421 tggaaaggat tattcgctgc agttgaggtt ctgccacttt tcaaaatata cctggacgaa
      481 gagaaaaata agattgatga aggatttaag ctttatacga cctggcacct gggttatcac
      541 atagcgctct ttaaaaacag gttttacctg gagccgcagg tccattgcaa ttactggccg
      601 gttgatacca aagcgccgca aggttttgaa gagaaggaaa gtaaatggaa gaattatttt
      661 ctgttcgaac ccaatctcta tttcggaatt aatttttaac aatcaggata tccgtaataa
      721 aaatcaaatt gtaaatacca tgaaaaaatt agcgctttcc tccgtcatca tactttcaac
      781 cctgagtatc acgttttcac aggttattcc tactgacagt ctctatttcg gccaaactcc
      841 tcccggttgc atcccccaat tatttgctcc gggcatcgtt tcgattcctg atcgaaatga
      901 agccaccatc actttctcac cggatggagc ctctgttttt tttcatattg agaaatatcc
      961 tgaacccgga aatccgtata tcctgtcggc tagctatctg gaagaccact ggacaacgcc
     1021 cgataccatc cctttctcca atggcagagc caccggcgaa ccattctttg cttacaatgg
     1081 caaccgggtt tacctgtttg ccaccaatgc tgttaaccat gaaggaattg ctgatctaag
     1141 cttttcacag aaagaagggg attcatggtc ggacccggtc agtcttggca atcctccaaa
     1201 ttctgaatct taccagtacc atccttgtat tgtcggggat tcgtcggtct atttttcgag
     1261 ccaggccggg aaaatatgcc ggagccagta ttgcgatggt atttatgaaa acaggatcgt
     1321 tttaccaaag cctgttaatt tcatcggttc acaaacctgg ggtgatccgt atgtatcagc
     1381 cgatgaaagc tatatgatca tgaaagcgat acgggaagaa ggatacgggc agaacgattt
     1441 gtatattgct tacagaaagc cggatggcaa ctggaccaat cccaaaaatc tcgggaatgt
     1501 catcaatact ccgggcgatg aaacttcggg ggatattacg cccgatggtt tgtacatgac
     1561 ctatggctca aatgacgatc tgtattgggt ttcggccggc tttaccgaca gccttcggta
     1621 taccaatttt attccgtata tgaaatatcc gatacctgat cagaccgctt atatcggaga
     1681 acaattcatt tactccattc ccgacagtac tttttctgat gatgattcca gcagtgtttt
     1741 cacctttacc gccgtactca cgaatggaaa tcccctgccg gagtggttga catttgacac
     1801 ggtttcggga gtttttacag gcgtgccgga gatggaacaa attctgaata taaaactgac
     1861 agccaacgac aacgccggtg catcggcatg gaccccattt aaacttagtg tgcaattgcc
     1921 aacaggatac gatccggtaa attttcatgc cctcacgatc tttccaaatc cgaaccgtgg
     1981 gcttatcaca attacattag gtccatttac cggcaagact gcgaccgtcg ttcttaatga
     2041 tcttcaaggc aggagttttg tccgacaagt gtttaaaaat gaaaccatta ttgatttgac
     2101 ggattgttcc agcggtgttt atatcctgaa agtgtatgtt gaaaatgaat taatttccag
     2161 gaagatttat aaaatgtagt gattctcaaa gaatttaaat ttaagcatat gaagcgattc
     2221 acaatagttt ttatagcagt agtcagtata ctacttgcaa atgcgcaaac aaagcttgat
     2281 tatagcatat ccgtaattta caacagatca agccaaacaa attataatcc ccaacttccg
     2341 gatgatggca cttttcaatg gaacgcctta aatacgttcg gaatcggtgt taatattggc
     2401 aaaccgctca gcgacagggt ctactggacg aatcgtcttc tctgccagca aaaagggtat
     2461 tcccaggaag ctcaaactgc agatttcatt gatgggattc ccggcccatt aagttatcat
     2521 aaacttcagg acagattcgg gtatctgact attgaatcga atatcggtta tcacatcatt
     2581 aaatttaaat ccctgaaatt aactcctctg atcggcttta atatcaatta tttgttgagt
     2641 aaatcgatag aatccgaagg ggtttacccc atcaattcct gttatcctat tgatgaatac
     2701 aatgggaact ggaaaaaaat aaatgtaggg tacaatgtcg gaatatcatg cgctattcag
     2761 aaaaaagtca atctggggtt tgaatttcag cgaagcataa ctcctttatt aaagactaat
     2821 gacttaacag taaaggactg gacgtggtcg gtcagactgg attttttact tagtgattaa
     2881 gaatgttttg gttaaaaatg tatctttaaa agcaaatttt taagcgtctg aattagccga
     2941 ctatatctaa ccacaaccgg taaatggacc acaaggagca gatcatagta tacttaatat
     3001 caaaaatcca tgaactgaag gagaagatca gggaggataa actgtcgaag gaggaactga
     3061 tcatctatat tgaaatgctg gaagatatgc tgagggatta tttcaacctc agcgactgat
     3121 tttgatgcaa cggagcagcc tgatcattca attttgtact ttcatagggt caatttatta
     3181 atcgaaacca agatggcgaa aatatcaatt caaccgggat tcagcataat tgtcccttat
     3241 aaggccgacg ataatacagg gaatgtcagg aagggacgag tggaagacaa cgacataatc
     3301 atcgtgaata acttcgataa gaagggcata aaaaccgagc aatcgtacta taatacatca
     3361 ggagaactta tagagcaggt agtctgtaaa ccggatgatt cgggaaagct tgccgaaaag
     3421 atctgtttat atggaataga aaatcctgac ataaagcccg atcctcattg ttcgaatctt
     3481 gactctggac gggaaggtac cgattctttt gagaactata cattttttcc agccagcgat
     3541 cagaaggtgt gcctgacttc atacaaatac gacaaaaagg gaagactcat tgaagaatac
     3601 cgctattccg acaacaggaa gctgatcaat aaagtggtca ttacctataa cgatcgtggt
     3661 gaagtacagg aatacatcca gtccgatcat ctgggatatc ccgatttcac gaacaaatat
     3721 cgttacgatg acgaccacag gctggttgaa gtcgaggaat ttaattcggt ggatgagctt
     3781 gcatgcaaaa cggtgaacac ttatgatgat cagggaaatc ttacagagaa aaggctttat
     3841 gatgagagag gcgaattatc aaaaaaaata aagcaggagt atgatgattc gggcaacctg
     3901 gttttgcagg aagagtacga tagaggtacc ttattgacga agacaaccag gtacagatac
     3961 aatgtaaaaa agatcctttt cgaaaaaata ctggatggat tctcaatggg cggcacttac
     4021 cggtatttgt atactctggg ggcgggaaat gcaagcacag aaaccgttgt cttcaaaaat
     4081 gacaaggcgt ataaaatgta tgagattcag tatggcctgt ctgccggtga ggtcaaacaa
     4141 aagctggctg ctttaaaatc cctgggccct gtcaatgtcc tgcacgactt gcgggatgag
     4201 tgggaagaga ataaaatgtt tatcattaac cggtttggaa aagaggctta tcagaatttc
     4261 ctggaacctt ataatattgt tcagattgag ttaaaccgcc tcttttcaaa gaaatccatt
     4321 cccaccacat acgatgatct taaaaagttc attgaaacta accaagagat ccgaaggatc
     4381 agcgattata atgcccctca tcagctggat catttttcac gggtgctgaa ggttcctaaa
     4441 tggaagctta agctgggaat gtgggccggt tcggatgaaa tggattacaa ggaagctatg
     4501 agaagcggtc gactgctgat ctggctcaat gcactggaat tttatgaagc tcgtttttaa
     4561 taacctttgc caaaccgata gattatgaaa tacttaaaga tcccttcccg cccgggccat
     4621 ctcagaatta ccgtcccgga tcaatttttt cacagcatta tcctgatcct gtttttgatg
     4681 actgtatgca gctgtgccag ccttgatagt acgaattaca agtcgcggct gagggcagtc
     4741 aaaaaaactg acgaccagag tattttgtac aaagtcgctc ttggagatgt gaaccgcgaa
     4801 gtcaaattgc ttgccttgag ccgaatcacc gaccaggaac ttcttggcaa atatgcggat
     4861 gagatgaatg atccgggcct gcagcaaatg gccatcggaa tgatcaccga ccaggagatc
     4921 cttttcgaaa tagcactgga tcatgctagt agtgatataa agatggcggc cttccataag
     4981 atcagcgata tgaaactggt taaccgcctg gctgtggaag ccaatgatcc gaaaattcag
     5041 cttcccgcca ttggcaagac agacagccag gaggtgctgt ttaaggcagc catggatgac
     5101 agccgcaccg aggtcaggca ggcggcattc gataaactga ccgatccggc gctgatcggt
     5161 aagctggcaa taggctcggg cgacacaaag ctccagctta aagcaataga gaaagtatcg
     5221 gaccagaata ccctctttga gattgctttg aaagaggatt ataaggaagc aaagcatgct
     5281 gccatgatcc ggttaacgga tgagcaactg ctgggcaagc tggccaatga aagcaaggat
     5341 actttcttca ggttaatggt aatcagtaaa ataactgacc aggatatact gtataagatt
     5401 gccttggaag atgaaaacag ggatgctcaa aaaacggccc tcagccggtt gtcgcctgaa
     5461 atgctcacca ggctggtgtc cgattacgat gaactggaaa tgcagttaaa agttatagag
     5521 atactggacg atcaaaccgg gctgatgaac atctcccaga accataccga ttggcaggtg
     5581 agaagggcgg ctttcacgaa gctggatgat gtttcacttg acttgctttc gcgtgaagcg
     5641 accgatccgg cgatcatgct aggggctaaa atccgccgcg gaattatcag ctg
//
LOCUS       DAOQXS010000136         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14518_length_5672_cov_3.404664, whole genome shotgun sequence.
ACCESSION   DAOQXS010000136 DAOQXS010000000
VERSION     DAOQXS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14518_length_5672_cov_3.404664"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="PKM34_05085"
     CDS             complement(<1..2010)
                     /locus_tag="PKM34_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ82996.1"
                     /translation="MKKLLLLSLSLLFTWAAFSQGALYTDDFEGYAVGDYIAESNPTW
                     WTTWSNDPGSGEDGYISDNFANSPTKSIFVDPDDGLTDLVLKLGNKTSGAYILSWYIY
                     VEAGYQGYYNVQHFESPGIEWAFDVWFYDDGTGELMVGNVYYPFTYSPGAWTLVEHEI
                     NIDADQAKLYVDGVQVHEWPFSYQTQETTGTNQLGGVDFWTDDATYRYYIDDLSFAPM
                     PITLYEDDFESYNVGDYVAESIPDWWTTWSNDPGSGEDGYFSGDFAHSPTKSIFVDPD
                     DGLTDLVLKLGNKTSGVYFLDWYMYVEAGYQGYYNFQHFESPGIEWAFDVWFYDDGTG
                     ELMVGNIYYAFTYNPSAWTKIEHYINIDEDIASLSVNGVQVHEWPFSYQTQETTGTNQ
                     LGGVDFWTDDATYRYYIDDLKFTQLGAESNPSIAVNPASISTSAAAGTTVTVPLTITN
                     NGTADLTYDLSVFYPMEGQNQPAPAIENPASHSRSLGYAMDNTTDPNYRPAAYNPVTD
                     DFLLHYDGDNADAFSWGPNPPYTIVNAAMFPPELTLPQAGTYITSVIMYFNMIGTDFR
                     VRIYGQGNGYQPGTMLVDQPFTPSEATWTEVELDNPVYITGEEIWVGYQYTQPSADMF
                     IPGVDAGPNNINGDWISFNGISWNHLTDYEDTRNWNIRAMLTGTPV"
     gene            complement(2120..3073)
                     /locus_tag="PKM34_05090"
     CDS             complement(2120..3073)
                     /locus_tag="PKM34_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNQ82997.1"
                     /translation="MQNKLYPWNHLRRFNSYAEHLKKQFGGRVQKLAIDAGFTCPNRD
                     GSKGIGGCSYCDNDAFNPSYCKPEKTVSQQIEEGIEFHRKRYRRAAKYLAYFQPYSNT
                     YAPLPRLREIYRQAIEIPEIAGIVIGTRPDCIDADKLEYLAELAKTHYVMLEFGIESC
                     YDETLRRINRGHTFPETVEALDMAKHYGINTGIHLIFGLPGETREMMLHQAEIISRLP
                     VKTIKFHQLQIIRGTRMEREYREKPDDFTLFTLEEYIDFVIDFTEKLNPSIVIERFAG
                     EVPPRFLAGPGWGLIRNDQILRMIESRMEERETWQGKRNRE"
     gene            complement(3063..4601)
                     /locus_tag="PKM34_05095"
     CDS             complement(3063..4601)
                     /locus_tag="PKM34_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNQ82998.1"
                     /translation="MMKQHPKGLLAAALANMGERFGFYTMMAILVLFLQAKFGLSGTH
                     AGIIYSVFYFSIYILAFVGGLIADKTRNYKGTIMIGQFVMAAGYLLIAIPTKTPVPNQ
                     VLFLIISCIGLFTIAFGNGLFKGNLQALVGQMYDNPTYGKMRDSGFSLFYMFINVGAL
                     FAPIAAIAMRNGWLMRHGYEYNPNLPELCHQYLKGTITPEAATKLQTLASEVSTAGPV
                     SDLTLFANDYLNVFATGFHYAFGISIIAMLISLTIYLVNKKIFPDPSLKVKTVVNGEH
                     HEITMDPKEVKQRLYALFAVFGIVIFFWFSFHQNGLTLTYFAKDYTDLSGIRINLGVM
                     EIKGAEIFQSINPIFIVLLTPIIMGIFGWLRARGKEPSTPKKIAIGMLIAATAYLVMT
                     IGSMGLPLKSEVEAAGGMADAERVTPMLLIGTYFILTVAELFISPLGISFVSKVAPPQ
                     YQGVMQGGWLGATAIGNQLLFIGAILYESVPIWLTWSVFVVACFLSFLTMLFMVRWLE
                     RVAK"
     gene            4771..5286
                     /locus_tag="PKM34_05100"
     CDS             4771..5286
                     /locus_tag="PKM34_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ82999.1"
                     /translation="MSPQEFKIEVIPLKNKLYRLSLRMLGATEEAEDAVQDAFVKLWT
                     KRNELSGYSSVEAFAMTVTKNLCLDRIKSKSFQTERISDKLNSSLGNDPGNVVENSDF
                     NGFIHRIIARLPDHQKVIMHMRDVEDYEYEQIAEVMGLNVNAVRVNLSRARKKVRDTI
                     LKMHDYELARN"
     gene            5267..>5672
                     /locus_tag="PKM34_05105"
     CDS             5267..>5672
                     /locus_tag="PKM34_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83000.1"
                     /translation="MNLQEIEKLLMKYFEGETSLPEEKILRDFFSGGDVPEKWMNLKV
                     YFSIIREEKKVLLENPELENRLETIPEAEKIAPLIDLRRPWIYWMTGVAASILILIAI
                     FVRFDPFPKKIEDTYKDPQVAYTEARKILQFVS"
BASE COUNT         1539 a         1306 c         1368 g         1459 t
ORIGIN      
        1 gacaggggta ccggtaagca tggcacggat gttccagtta cgggtatctt cataatcggt
       61 cagatggttc caggaaatcc cgttgaatga gatccagtcg ccgttgatat tattaggacc
      121 ggcatccaca ccggggataa acatgtcggc agacggctgt gtgtattggt aacctaccca
      181 gatctcttct ccggtgatat acacgggatt gtccagttca acttcagtcc atgtagcttc
      241 agacggggtg aacggctggt cgaccagcat ggttcccggc tggtaaccgt tgccctgacc
      301 gtaaatacgc accctgaaat cagttccgat catattgaag tacatgatca cagatgtgat
      361 ataagttccg gcttgcggaa gtgtcagttc gggtgggaac atagcggcat ttacaatggt
      421 gtacggcgga ttgggccccc acgagaatgc atcggcattg tcgccgtcat aatgcaggag
      481 aaaatcgtcg gttacaggat tatacgcagc ggggcgataa tttggatccg tagtattatc
      541 catggcatat cccagtgacc tggaatgaga agccggattt tcgatagctg gtgccggctg
      601 gttctggcct tccatcgggt agaagacgct cagatcataa gtgaggtcgg ctgtcccgtt
      661 gttggtgatg gtcaaaggta cggtcaccgt tgtgcctgct gcagcagatg ttgagatgct
      721 ggccggattg acggcaatag aaggattgga ttctgctcca agctgggtga atttgaggtc
      781 atcgatgtaa tacctgtagg tcgcatcatc tgtccagaaa tcgacaccac ctagttgatt
      841 ggtaccggta gtttcctgtg tctggtaaga gaaaggccat tcatgtacct ggaccccgtt
      901 gacggacagg ctggcgatat cctcatcaat gttaatataa tgctcaatct tagtccatgc
      961 tgatggattg taagtgaaag cataataaat atttccaacc atcagttcac cggttccatc
     1021 gtcatagaac cagacatcga aagcccattc gataccgggc gattcaaaat gctggaaatt
     1081 gtaatagccc tggtatccgg cttctacata catataccag tcgaggaaat atacaccgct
     1141 tgttttgttc ccgagtttca gcaccaggtc ggtgaggccg tcgtccggat ccacgaaaat
     1201 ggattttgtg gggctgtggg caaaatcacc ggaaaaatat ccgtcttctc ctgaaccggg
     1261 atcattgctc caggtcgtcc accagtcggg aatgctttcg gcaacataat caccgacatt
     1321 gtaagattcg aagtcatcct catagagcgt aatgggcatg ggtgcgaaac ttaaatcatc
     1381 gatgtaatac ctgtaggtgg catcatctgt ccagaaatcg actccgccaa gctggttggt
     1441 accggtagtt tcctgtgtct gataagagaa aggccattcg tggacctgga cgccatcaac
     1501 atatagtttt gcctgatcag catcgatatt gatttcatgc tctaccaaag tccacgctcc
     1561 cggagagtaa gtaaaaggat aataaacatt gcctaccatt aattcaccgg ttccatcatc
     1621 atagaaccac acatcaaagg cccactctat tccgggcgat tcaaaatgct gaacgttgta
     1681 atacccctgg tacccggctt ctacatatat ataccaactc aggatatatg ctccgctggt
     1741 cttatttccc aatttcagca ccaggtcggt aaggccgtcg tccggatcga caaatatgga
     1801 tttggtcggg ctgttggcga aattatcgga aatgtatccg tcttcgcccg aaccgggatc
     1861 attgctccag gtggtccacc aggtcggatt actttcagcg atgtaatctc ccaccgcata
     1921 gccttcaaaa tcatcggtgt acaaagcacc ttgtgagaag gcagcccagg tgaagagcaa
     1981 cgataacgat agtagtaata actttttcat aaagctttat tttttggtta acactataag
     2041 aatgcacaaa ggcaaatata aaaagataat tccggaaatg acagaaaaaa aaccaccaaa
     2101 caaattcaac cctattttac tattccctat tcctttttcc ctgccacgtc tccctttcct
     2161 ccatccttga ctcaatcatc ctcagtatct gatcattccg tataagcccc cagcccggac
     2221 cggccaggaa tcggggaggt acctcgccgg caaaccgttc gataacgatg gaagggttta
     2281 atttttcggt aaagtcgatc acaaaatcga tatactcttc cagggtgaaa agggtaaagt
     2341 cgtccggttt ttccctgtat tcccgttcca tcctggtacc ccggatgatc tgtaactgat
     2401 ggaattttat agtcttaacc ggaaggcgtg aaatgatttc tgcctgatgt agcatcattt
     2461 cgcgtgtttc gccgggaagt ccgaagatca ggtgaatacc cgtattgatg ccgtaatgtt
     2521 ttgccatatc cagggcttcc accgtttccg gaaacgtatg acccctgttg atccggcgaa
     2581 gtgtctcgtc atagcacgat tcgatcccaa actcaagcat cacataatgt gttttggcta
     2641 attccgcaag gtattccagc ttgtcagcgt cgatacaatc gggccgggtt ccgatcacaa
     2701 tccctgctat ttcaggaatt tcgatggcct gccggtagat ttcgcgaagc ctgggcaggg
     2761 gagcataggt attcgaataa ggttgaaaat aagccaggta ctttgcagcc cgccggtatc
     2821 tcttcctgtg aaattcaatg ccttcttcaa tctgctgact gaccgttttt tcgggcttgc
     2881 aataagaagg gttgaaggcg tcgttatcac aataggaaca accgccaatg cctttggacc
     2941 catcgcggtt gggacaggtg aagcccgcat cgatggccag tttctgcacc cttcctccga
     3001 actgcttttt cagatgttcg gcataggaat taaaacgacg gagatgattc cagggataca
     3061 acttattttg caacccgttc cagccatctt accatgaaca acatggtcag gaaggagagg
     3121 aaacaggcta cgacgaatac gctccaggtc aaccagatcg gcacgctttc gtaaagaatg
     3181 gcgccgatga acagcaactg gttcccgata gcagtcgccc cgagccatcc tccctgcata
     3241 acgccctggt actgcggcgg agctaccttt gacacaaatg aaatgcccag cgggctgatg
     3301 aaaagctctg cgaccgtcag gatgaaatag gtgccgatca gcagcattgg cgtgacgcgc
     3361 tcggcatccg ccattccgcc ggctgcctcg acctccgatt taagcggtaa tcccatggat
     3421 ccaatagtca tgaccagata agcagtggct gcgatcagca tcccgatagc gattttcttc
     3481 ggagttgatg gttctttgcc ccgggctcgg agccatccga aaattcccat gataataggc
     3541 gttaacagga cgataaatat tgggttgatc gactggaaaa tttccgctcc cttgatctcc
     3601 atgactccaa gattgatccg gataccactg agatcggtat aatctttggc gaaataggtg
     3661 agtgtcaatc cgttctgatg gaaagagaac cagaagaaga taacaatgcc gaacacagcg
     3721 aacaaagcat acaatctctg tttgacctct ttgggatcca tggttatttc atgatgttca
     3781 ccattcacca ctgttttgac cttgagcgac ggatcgggga aaatcttctt gttgaccagg
     3841 taaatcgtca gggagatcag catggcaatg atcgaaatac caaaagcata atggaatccg
     3901 gtagcgaaaa cgttaagata atcattggcg aataatgtca ggtcgcttac cggtcccgca
     3961 gtgctgacct cggaggccag ggtctgcagt ttggttgctg cctccggcgt gatcgtgccc
     4021 ttgagatact ggtggcaaag ttccggcagg ttgggattgt actcataccc gtggcgcata
     4081 agccagccat tccgcatggc gatggcggca ataggtgcaa acaaagcccc gacattgatg
     4141 aacatgtaaa agagtgaaaa tccggagtct ctcatttttc cgtatgtggg attgtcgtac
     4201 atttgaccga ccagcgcttg caggttgcct ttgaacaatc cattaccgaa agcaatagta
     4261 aacagtccga tacaactgat gataagaaac aagacctggt ttggcaccgg cgtctttgtc
     4321 gggatggcaa tcaacaggta accggctgcc atgacaaact ggccgatcat gatggtcccc
     4381 ttgtaatttc ttgtcttgtc agcaatcaat ccgcctacaa aagccagaat gtagattgaa
     4441 aaatagaata ctgaataaat tatccctgca tgagtgcctg aaaggccgaa ttttgcctgt
     4501 aagaacaata cgaggatcgc catcatggtg tagaaaccga acctttctcc catgttggcc
     4561 agggctgcag cgagcaaccc tttgggatgt tgtttcatca taactttaga ttttagtttg
     4621 tttatagatt taatttgaat ccggaaagtt taaggatgac aaaaataatt aaaaatcgat
     4681 cttttcaaag ttccgttcag ttttcggcaa aatttgcaga aatttataca ggtgatgtaa
     4741 tgaagtctgg taaaattcgt cttacccgtg atgagtccac aagagtttaa aatagaggtc
     4801 attccattga aaaacaagtt gtacaggctt tcgctgagaa tgctcggagc aacggaggaa
     4861 gcagaagacg ccgttcagga tgcctttgtt aaactctgga caaaacgaaa tgaacttagt
     4921 gggtattcca gtgttgaagc ctttgcaatg actgtgacaa aaaatttgtg ccttgaccgg
     4981 atcaaatcga agagttttca aaccgaaagg atttcggata aactgaattc atcccttgga
     5041 aatgacccgg gtaacgtggt tgagaattcg gatttcaatg gattcatcca ccggatcatt
     5101 gcccggttgc ctgatcatca aaaggttatc atgcacatga gagatgttga agattatgaa
     5161 tacgagcaga ttgccgaagt tatggggctg aatgtcaatg cggtaagggt gaacctgtca
     5221 agggccagaa agaaagtcag ggatacgatc ttaaaaatgc acgattatga acttgcaaga
     5281 aattgaaaaa ttactgatga agtatttcga aggagagact tcccttccgg aagagaaaat
     5341 tctgagagat tttttctctg gcggcgatgt tccggaaaag tggatgaatc ttaaggttta
     5401 cttttcaata atcagggaag aaaagaaggt gcttctggaa aatccggaac tggaaaaccg
     5461 gttggaaacc attcctgaag ctgaaaagat cgccccgctg atcgaccttc gccggccctg
     5521 gatctattgg atgactggtg tcgccgcctc catcctgatc ctcatcgcca tctttgtccg
     5581 gttcgatcct ttcccgaaaa aaattgaaga tacgtataaa gatccacagg tagcatatac
     5641 ggaagcgcgg aagatacttc agttcgtatc ct
//
LOCUS       DAOQXS010000137         5669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14527_length_5669_cov_3.654079, whole genome shotgun sequence.
ACCESSION   DAOQXS010000137 DAOQXS010000000
VERSION     DAOQXS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14527_length_5669_cov_3.654079"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="folK"
                     /locus_tag="PKM34_05110"
     CDS             <1..431
                     /gene="folK"
                     /locus_tag="PKM34_05110"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNQ83001.1"
                     /translation="KDEIYLSIGKIVAQSSLYESEPWGFQAEKCFLNQVVKTETDMEP
                     AEILNEILSIENKLGRIRNNTGTYQSRTIDIDILFYNDQIVTEKNLVIPHPKIEERMF
                     TLVPLAELDQSFVHPASRKTIGQLINECTDSISVYLYHPD"
     gene            436..1083
                     /locus_tag="PKM34_05115"
     CDS             436..1083
                     /locus_tag="PKM34_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNQ83002.1"
                     /translation="MNHPLLKYNFIAIEGNIGAGKTTLATRLSDDYNARLILERFENN
                     SFLPKFYEQPDAFAFPLEMSFLADRYQQLKDKLAMPELFSTQTISDYFIDKSLIFARN
                     NLGSDEFNLYARLFQIISAFLPRPDLLVYLYLDIDGLLKNIKKRGRDYEQNIQPGYLE
                     QIQTGYLNHFRVLSGMRILIIDTGRVDFMENESDYRSFLEIINMDYEPGIHRILL"
     gene            complement(1137..3557)
                     /locus_tag="PKM34_05120"
     CDS             complement(1137..3557)
                     /locus_tag="PKM34_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83003.1"
                     /translation="MRKAYNFLFKCLIAVMIGILPVLTYAQDQGDQTKETRKEKRQDF
                     KHYFYLNGNIGGMFNHTDVAENRFFGFDGIGLGYGGSFGWQFKPIWGVRIKGAAGKVS
                     GDWDGNTFSNNLFDYHLDVTINWSNLISGYRGDRLLDIYSAHGYGQVQAKDKDVETDY
                     RIYAEYNSGIGGAFHLAPKWDLNVEWVMNLTETDKFDLYNPGNSIVIGKVETWDLFSY
                     ASVGVTYKFGQGDPLKKMAKNFETVTFKAEPDPLEAHGGKVPVKITGNFPEGYFHPKA
                     ALWAEPVLVCDGTRIPMKPILLKGTSVGGEGIVISKDGGSFTYETVVDYNENCRAAEL
                     ILEPIIFVPKEDLPADLKKEDVLGYKKNIELPARKLADGVIVTPSRFAFNKTGSIVPH
                     GYVKEVIISKQANLYFEVNKHNLNMNLPLNKLDENKAKFTELYDFINLGYQIKDISID
                     GWASPEGEETFNQGLSENRTKTGHKFIVDKMKGLIKAKGSKLTIKDAEKEIRYNLLHH
                     GPDWNGFIAALQASDIKEKSSILNVINSAGTPAKKEEEIRNMIRIYPEIEAKLLAPLR
                     RAEIVVNCYEPRRTDEEIIALSTTKPGDLNEKELLYAASLQQDNKVKLDIYKSAINLF
                     PDSYKGYANAGAIEVEIGDTKAAKAHLEKAAALNANSGEVHNNLGIVYGLEGDIAKAE
                     EHFTKAGQLGTNADYNLGVIHIYKGEYGKALSKFGNKTCDYNVALAYIAQKNYPPAQK
                     QLDCASKDAQTHYLMAILGSRTSNTGMLFENLGKAIQMNEAYKAEAQIDREFVKYFTD
                     PNFTSLVK"
     gene            complement(3789..5327)
                     /locus_tag="PKM34_05125"
     CDS             complement(3789..5327)
                     /locus_tag="PKM34_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNQ83004.1"
                     /translation="MLVKTFGCAIYGINAVTVTVEVNIEQGINFFMVGLPDSAVKESH
                     QRIEAALRYIGYRVPGKKIVINLAPADIKKEGSSYDLAIAVGILGANGNLVTDQVSEY
                     IIMGELSLDGGVMPIKGALPIAIEARRKRFSGLILPAANAREAAIVEGIEVYGVENIV
                     DIIDFFNGKRQLIAVKVNIEEEFFKNLNNYEFDFADVKGQENIKRALEISAAGGHNAI
                     LIGPPGSGKTMLAKRLPSILPPLSLEEALETTKIHSVAGKLRRHDALVTVRPFRAPHH
                     TISDAGLVGGGSFPQPGEISLAQNGVLFLDELPEFKRSVLEVLRQPMEERVITISRAR
                     SAIEFPANFMLVAAMNPCPCGYYNHPTQDCTCSAGNVQKYLNKISGPLLDRIDLHVEV
                     VPVPFRELTNFTISESSQVIRDRVIAARKVQEQRFRGMHGIHCNAQIPSKKLHSLCTI
                     SEEGHVLLKNAMERINLSARAYNRILKVARTIADLDGCAPIRTEHLAEAIQYRSLDRE
                     SWGS"
BASE COUNT         1377 a         1385 c         1276 g         1631 t
ORIGIN      
        1 cgaaggatga aatttatctg tcgatcggaa aaattgtagc ccaatcgtca ctctatgagt
       61 ctgaaccctg gggttttcag gctgagaaat gttttctcaa ccaggtcgtt aaaacggaaa
      121 cggacatgga gccggctgag attcttaacg aaatcttatc tatcgagaat aagctgggta
      181 gaatccggaa taatacggga acgtatcaat cgaggactat tgatattgat atcctttttt
      241 ataatgacca gattgttacc gagaagaatc tggttattcc acacccaaag atagaggaga
      301 ggatgttcac ccttgtaccc ctggccgaac ttgatcaatc ctttgttcac cccgcaagcc
      361 ggaagacaat cgggcaactg atcaacgaat gtacggattc gatatctgtc tatctttatc
      421 atcccgattg acgctatgaa ccatccattg ctgaaataca acttcattgc catagaaggc
      481 aatatcggtg ccggcaagac aacgcttgcc acccggttgt cagatgatta taatgcccgc
      541 ctgatactgg aacgatttga aaataactcc ttcctgccca aattttatga acaaccggac
      601 gcttttgcct ttccgctgga gatgtccttc cttgccgacc gttatcagca actgaaggat
      661 aaactggcca tgccggaact attctccaca cagactattt cggattattt tatcgacaag
      721 tctttgattt tcgccagaaa taatttgggc tccgacgaat ttaacctgta tgcccgtctt
      781 ttccagatca tcagcgcatt tcttccaaga cccgatctgc ttgtttattt gtacctggat
      841 attgatggcc tcctgaaaaa tatcaagaag cgggggaggg attatgaaca aaacattcag
      901 cccgggtacc tcgagcagat ccagacgggc tatttgaacc attttcgtgt cctatccggg
      961 atgaggatcc ttatcatcga caccggaagg gtggacttca tggagaatga atccgattac
     1021 aggtccttcc tggaaatcat caatatggac tacgagccgg gaatacaccg gatcctgcta
     1081 taaaaaaaga ggctgccact ttcaaagggc agcctcttgt gttttacacc atctccttat
     1141 tttacaaggc tggtaaagtt aggatcggtg aaatatttaa caaattccct gtcgatctga
     1201 gcttcagctt tataggcctc attcatctgg atagctttcc ctagattttc aaataacata
     1261 ccggtattgc tggtccttga gccaaggata gccatcaggt agtgggtttg tgcatccttg
     1321 gaagcgcaat ccagttgctt ctgtgccggc gggtaattct tctgtgcgat gtaagccaga
     1381 gccacattgt aatcacaggt cttattcccg aatttgctca gtgctttgcc gtattcgcct
     1441 ttataaatat ggatcacacc caggttgtaa tcggcgttcg ttccgagctg gcctgctttg
     1501 gtaaagtgct cttccgcttt agcaatatct ccctctaagc catacacaat tccgaggttg
     1561 ttgtgtacct ctccgctgtt ggcatttaag gcggcagctt tttcaagatg ggctttggca
     1621 gccttggtat cacctatttc cacttcaatg gcaccggcgt ttgcatagcc cttgtagctg
     1681 tcggggaata agttgattgc cgatttataa atatcgagct tgactttatt gtcttgttgc
     1741 agcgaagcag catagagaag ttctttttcg ttcaggtcac ccggttttgt cgttgacagg
     1801 gcgatgatct cttcgtcggt tctcctgggt tcgtagcaat tgaccacgat ctcggccctt
     1861 ctaagcggag ccaggagttt agcttcgatt tcaggataga tgcggatcat attcctgatt
     1921 tcttcttctt tctttgccgg tgtacccgct gaattgatca cgttcaggat gctgctcttt
     1981 tccttgatat cggaagcctg aagagcggcg atgaatccgt tccagtcagg tccgtggtgc
     2041 aacaggttgt acctgatctc tttttcagca tctttaatgg tcagcttgct tccttttgcc
     2101 ttgatcaggc ctttcatctt gtcgacaata aatttgtgac cggtcttggt tctgttctcc
     2161 gacagtccct ggttgaaagt ctcttcccct tcgggagatg cccagccgtc gatgctgata
     2221 tctttgatct ggtaacccag gttgataaag tcatacagct ctgtaaattt cgctttgttt
     2281 tcgtccagct tgttcagtgg cagattcatg ttaaggttat gcttgttcac ctcgaaatag
     2341 aggttagctt gtttgctgat gataacctcc ttcacatatc cgtgggggac gatggaacct
     2401 gttttgttaa aggcaaaccg gctcggtgtt acgataacgc cgtcagccag tttacgggcc
     2461 gggagttcga tattcttttt gtaaccgagt acatcttcct tctttagatc agccggaagg
     2521 tcttccttcg ggacaaagat gatgggctcc aggataagtt ccgcagcgcg gcaattttca
     2581 ttgtaatcta caacagtctc ataggtgaat gaaccgccgt ctttggaaat tacgattcct
     2641 tctccgccga ccgaagtacc tttcagcagg atgggtttca tcgggatgcg ggtgccgtcg
     2701 caaaccagga ccggctcagc ccacaaagcg gctttcgggt ggaaatagcc ttccgggaaa
     2761 ttgccggtga tcttcacggg gacttttcct ccgtgggctt ccagcggatc gggttcggct
     2821 ttgaacgtga cggtctcgaa attcttcgcc atcttcttca gcggatcgcc ctggccgaat
     2881 ttgtaagtta ccccaacaga agcgtagctg aatagatccc atgtttccac cttgccaatt
     2941 acaatgctat taccgggatt atacaggtca aatttatctg tctcggtcag gttcataacc
     3001 cactcgacat tcaggtccca cttgggagcc agatgaaatg ccccaccgat tccgctgtta
     3061 tattcggcgt agatcctgta atccgtttca acatctttat ctttggcctg cacctgcccg
     3121 taaccatggg cgctgtatat atccagcaaa cggtcgccgc ggtatccgga aatcaggttg
     3181 ctccagttga tggtcacatc cagatggtaa tcgaacaggt tattgctgaa tgtgttgcca
     3241 tcccagtcgc ccgacacttt gccggcagcg cctttaatcc tgacgcccca gatcggtttg
     3301 aactgccagc cgaaactgcc gccgtaaccc aatccgatgc catcaaagcc gaagaatctg
     3361 ttctcagcta cgtcggtgtg attgaacatc ccaccaatgt taccgttcag ataaaagtaa
     3421 tgtttgaaat cttgtctctt ctctttgcgg gtctctttgg tctggtctcc ctggtcctgt
     3481 gcatacgtca gaaccggcaa aatgccaatc attacggcaa tgaggcattt aaataagaaa
     3541 ttgtaggctt ttctcatatg gattaatttt aagtttttcg ggtttctttt ctaatttatt
     3601 tgcaaatata aacagttata taaacaatcc aaaacagtta taggctaaaa ataatgaatt
     3661 aatcgctgat aaagttaaaa ttaaattgaa atttaccagc aataatatat ctgtaaatat
     3721 aaatgatttt tataaactct tccatcgaac atcgaaaatc gtcaatcgaa aatcgaaaat
     3781 cctcacaact agctccccca gctttccctg tccaggctcc ggtattgtat tgcttctgcc
     3841 agatgctccg tccggatggg ggcacaacca tcaaggtcgg caatagtgcg ggctactttt
     3901 aaaatccggt tgtaagcccg cgccgaaagg ttgatcctct ccattgcatt tttaagcaat
     3961 acgtgacctt cttcagaaat cgtgcatagt gaatgtaact tcttacttgg gatctgtgcg
     4021 ttgcaatgaa tcccgtgcat cccgcggaac cgttgttcct ggaccttcct ggccgcgatc
     4081 acccggtcgc ggatgacctg gctggattcg gaaatggtaa aattcgtcag ctcacgaaaa
     4141 ggtaccggca ccacttccac atgcagatcg atccggtcga gtaacgggcc ggaaatctta
     4201 ttcaggtact tctgaacgtt ccctgccgaa caggtacaat cctgcgtcgg atggttgtag
     4261 tatccgcacg ggcaggggtt catcgcggct accagcatga agttggcggg gaattcaata
     4321 gccgacctgg cccttgaaat ggtgatcacc cgctcctcca tcggttgcct gagcacttcc
     4381 agcacggatc gcttaaattc gggcagttca tccaggaaaa ggaccccgtt ctgggcaaga
     4441 gaaatctcgc caggctgggg aaatgaccct ccacctacca ggccggcatc gctgatggtg
     4501 tgatgcgggg cccggaacgg ccggacagtg accagcgcat catgacgcct taacttgccg
     4561 gcaaccgagt ggatcttggt ggtttcgagc gcttcctcca ggctcaacgg aggcaggatg
     4621 gaaggaagac gtttggccag catggtcttc ccagatcccg gagggccgat cagtattgca
     4681 ttatgacctc cggcagctga aatctccagc gcccgcttga tgttttcctg ccccttgaca
     4741 tcagcgaagt caaactcata attgtttaag tttttgaaaa actcctcctc aatgttaact
     4801 ttaacagcga tgagctgtct tttgccattg aagaaatcga tgatatccac gatgttctcc
     4861 acaccgtaaa cttcaattcc ttcaacaatg gctgcttcgc gcgcattggc ggccggcagg
     4921 ataagcccgc tgaatctttt cctccgggct tcaatagcaa taggcagtgc gcctttgata
     4981 ggcataaccc cgccatcgag cgacaattct cccataatga tgtattcgct cacctggtcg
     5041 gtgaccaggt ttccgttggc tcccagaatg cccacagcga tggccaggtc gtacgacgaa
     5101 ccttccttct tgatgtccgc cggggccagg ttgataacga tcttttttcc gggcacccgg
     5161 tatccgatat agcgcagtgc ggcctctatc cgctggtggc tctcttttac cgcgctgtcc
     5221 ggtaatccta ccataaaaaa attaattccc tgttcgatgt tcacttcaac agtgaccgta
     5281 acggcattga tgccatagat ggcacaacca aaagttttaa cgagcatatt tctccggatt
     5341 taaagtttca gactctgatt tctttatctt aatccggaaa tcgggtaaaa aatacccggt
     5401 cgaaggatga attttttttt attattgctt attgcttgat tgtgagaggt ttttacgaca
     5461 gatacatgac agaaataatg gaatgctgac gaacatttat aaagcacttc taattaaagg
     5521 gtcattaaaa cttgctacat ttgctctgta taatcgaaaa tctttattcc tctttcttaa
     5581 ccgataagaa taagctgaat gaaaattata atacgctcat tattggttat tcttattttc
     5641 tcattttccc gactgctgtt ttctcagga
//
LOCUS       DAOQXS010000138         5649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14592_length_5649_cov_3.521094, whole genome shotgun sequence.
ACCESSION   DAOQXS010000138 DAOQXS010000000
VERSION     DAOQXS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14592_length_5649_cov_3.521094"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PKM34_05130"
     CDS             <1..1337
                     /locus_tag="PKM34_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ83005.1"
                     /translation="TPEWAYGITTIKPHIIRETARAMANASPAVIVHPGRHVTWYGDD
                     TQRSRAIAILNALLGSWGRRGGFYFKESLSIPDFPHPEYPKPAWSWKDTLDGKYPLAR
                     LSVANAIVDASIPAEGKEHTIRGWIVSGTNLPMTMPQKDQLLKAMQALDLLVVVDTMP
                     MEITGYADVVLPECTYLERYDELRDSPAREPFLGFRMPAATPMYESKPAWWIAKQLGE
                     RLGLGAYFNYTDYKEVLAWQLNQLGTSIEEMERMGVKKFERSSGSLYMEEGQPMEFNT
                     NSGRIELYSTELASLGFDPMPVYTAHPEPPPGFYRLIYGRAPMHTFSRTANNPNLVDL
                     MKENSVWIHPDVADIWGLKKDQYIWLKNQDGVTSHFPIKVRITERIRWDSVYMVHGFG
                     HQDKKLSLAYGRGANDSDLITEVKVDPLMGGTGMRGNFVTILTDKPGKETES"
     gene            1334..1867
                     /locus_tag="PKM34_05135"
     CDS             1334..1867
                     /locus_tag="PKM34_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ83006.1"
                     /translation="MRYAMAIDTRKCVGCSDCVVACQLENNVPVGFCRDWVTESVHGS
                     YPNVELEIRSERCNHCENAPCVRCCPTGASHIVEGGIVLVTGNKCIGCGACIQSCPYD
                     ARYSHPEGYVDKCTFCHHMVSKGGDPACVAVCPTKCMYFGDLDDPNSKVSNALKNRKF
                     KVLAPEAGTNPQIYYLI"
     gene            1885..2826
                     /gene="nrfD"
                     /locus_tag="PKM34_05140"
     CDS             1885..2826
                     /gene="nrfD"
                     /locus_tag="PKM34_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNQ83007.1"
                     /translation="MKEELFVSGRDIPNIDPFLNIWHWQIPIYLYLGGLAAGILFFAG
                     IFTILNKEKEMPATVKWAPMLVPGALVIGLLMLFLDLKHQLFFWRLYTTVRFESPMSW
                     GAWVLMAVTPLSFIWAATYIKEIRPEWNWKFNLLEKFEAFAVKNRKPMAWIFIVLSVV
                     LGIYTGILLSAFNARPLWNNSILGPLFLVSGLSTGTAVIMWITKNPRERIIMSRIDLV
                     LIIVELFFITHMMMGMLAGSSVQIAAAGLFLGGQFTVSFWVFVIVLGLVFPAVLEILE
                     LAGFKIPVAIPALLILFGGLVFRFIMVEAGQITRYLY"
     gene            2845..3378
                     /locus_tag="PKM34_05145"
     CDS             2845..3378
                     /locus_tag="PKM34_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ83008.1"
                     /translation="MMENNRKDRSQNYLNPYFGGVLLGIVLILTFYITGRGLGASGAA
                     KSTVVTTIQSVAPEYAKKSHYVSQFISDDNHPMNNWLVFEVLGVFAGAFLSGTLYGRL
                     KPRVEHSPKITSKKRLVVALLGGLLFGFGSQVARGCTSGAALSGMAVLSFGGIITMMG
                     IFGSAYLFSWIFKRLWI"
     gene            3390..3980
                     /locus_tag="PKM34_05150"
     CDS             3390..3980
                     /locus_tag="PKM34_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ83009.1"
                     /translation="MGPLIPNDIIPADWNNAIALLLGMAFGFVLEASGFSSSRRIMGT
                     FFGYDFVVLRVFFTAAVTSMIGILYLDYFGWVDFGQLYIHPTYLMSAIIGGVIMGFGF
                     ALGGYCPGTSYCAVAIGKLDALAYSIGLFIGIYLFTFAYPLLEGLYNGSFMGYVTAPE
                     VMGINQYLFVFFVVIAALGMFFVATIVQRKVKKVEY"
     gene            3992..4606
                     /locus_tag="PKM34_05155"
     CDS             3992..4606
                     /locus_tag="PKM34_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ83010.1"
                     /translation="MSRKYIIPVALLLILSIVLVLLPRKQDHSQMSPEELLRQINSTS
                     RFVSTDEVADRLIRKDPSLALIDVRNDKLFSEYSLPGALNIPVDNILADESQEILSQE
                     GLEFVFYSTDDILADQTWIILKRKGLKNIYVLKGGLNEWFRTFFTNPPPAETADSEEF
                     ALYQFRMGVRQYFTGTPADSPEKSSAEKIIVTPKKKKSAAEGGC"
     gene            4626..5264
                     /locus_tag="PKM34_05160"
     CDS             4626..5264
                     /locus_tag="PKM34_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ83011.1"
                     /translation="MKELNRTDRLTIATTMLAIILIIGLVTMRKPAVEFAIPATEVTK
                     LATDPSYVIPVTILAEMMKNNDPALALIDVRNPVKFRQEHIPSALNIPSADLLSEQNL
                     NLIKNLAEEDKKIIIYGGNHLEANGSWLILKQLGINNLYILDAGMEGYNLYRQNLSVN
                     LPFEDVKSERPAYNYNEILNSFGTSSSAQETGKTEPVRITKKAKKSAAEGGC"
     gene            5267..>5649
                     /locus_tag="PKM34_05165"
     CDS             5267..>5649
                     /locus_tag="PKM34_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNQ83012.1"
                     /translation="MPEEISNKKDKGKAAEKEMSFWEHLEELRWHLLRSAVVLVVLAI
                     GAFLMRRFIFDVIILAPKEPGFISYRALCTIGKWLHTDSLCLDIEPLQIVNIQMSGQF
                     MVHLYISLMTAVVVALPYLLWEIWS"
BASE COUNT         1379 a         1440 c         1434 g         1396 t
ORIGIN      
        1 tgacgccgga atgggcttac ggaattacca cgatcaagcc tcacattatc cgcgaaacgg
       61 cacgggccat ggccaacgcc tctcctgccg tcatcgtgca tcccggaagg catgtcacct
      121 ggtatggcga cgacacgcag cgctccaggg ccattgctat cctcaacgct ttgctgggct
      181 cctggggtcg ccgcggaggg ttctatttca aagagagcct ttccattccc gactttccac
      241 acccggaata tccaaaaccg gcctggtcat ggaaagatac actggacgga aaatatcctc
      301 tggcgaggct ctccgtcgca aacgccatcg tcgatgcctc cattcctgcc gaaggaaaag
      361 aacatactat ccggggatgg atcgtcagcg gtaccaacct gccgatgact atgccgcaaa
      421 aggatcagct cctcaaagcc atgcaggcgc tcgatctgct ggtggttgtc gatacgatgc
      481 ccatggagat caccggttat gccgatgtgg tattgcccga atgcacctac ctggaacggt
      541 atgatgaact gcgcgattct cctgcacgcg agccattcct gggattccgc atgccggccg
      601 caacaccgat gtacgaaagc aaaccggcct ggtggattgc caaacaactt ggagaacggc
      661 tgggactggg cgcttatttc aattataccg attacaaaga agtgctggcc tggcagttga
      721 accagcttgg aacgtctatc gaagaaatgg agcgtatggg tgttaaaaaa tttgaacgct
      781 cttccggatc gctctacatg gaagagggcc agcccatgga attcaatacc aattcaggca
      841 ggatagagct ttacagcaca gaactggcat cgctcggctt cgatcccatg ccggtttaca
      901 cggcgcatcc cgagcccccg cccggatttt accggctgat atacggccgg gctccgatgc
      961 acactttcag ccggacggcc aataatccca acctggtcga cctgatgaaa gaaaactcgg
     1021 tgtggataca tcccgatgtg gcagatatct ggggattaaa gaaagatcag tacatctggc
     1081 ttaagaacca ggatggggtt acgtcgcact tcccgataaa agtcaggata accgaaagga
     1141 tccggtggga ttcggtttat atggtacatg gtttcggaca ccaggataaa aaactaagcc
     1201 ttgcttacgg acgtggggca aacgattccg acctgattac cgaggtgaag gtggatccgt
     1261 taatgggagg cacaggcatg agaggaaatt ttgtaacgat cttaacggat aaaccgggaa
     1321 aggagactga atcatgagat atgcaatggc tatcgatacc cggaaatgcg taggctgcag
     1381 cgactgtgtc gttgcatgcc agctggaaaa taatgtgccg gttggctttt gccgggattg
     1441 ggtgaccgaa tcggtccacg gaagttatcc gaatgtcgag ctggagatcc gttcagaaag
     1501 gtgcaaccat tgtgaaaatg ccccttgcgt gaggtgctgc ccgacaggcg ccagccatat
     1561 tgtggaaggg ggaatcgtgc tggtgaccgg gaacaaatgc atcggctgcg gggcctgcat
     1621 ccagtcgtgt ccgtacgatg cccgctattc gcacccggaa ggctatgtgg ataaatgcac
     1681 cttctgccac cacatggttt caaagggagg tgatccggcc tgtgtagcag tctgcccgac
     1741 caaatgtatg tacttcggcg acctggatga ccctaacagt aaggtttcca acgcattaaa
     1801 aaacaggaaa ttcaaagtac ttgccccgga agccgggact aatccgcaga tttattatct
     1861 aatttaatct ctttataact ggatatgaaa gaagaattat tcgtcagcgg tcgcgatatt
     1921 cccaatatcg acccttttct gaatatctgg cactggcaga tccccatata cctttacctg
     1981 ggtgggcttg cggccggcat tcttttcttt gcgggaattt ttaccatcct gaacaaggaa
     2041 aaagagatgc cggcaacggt aaagtgggca ccaatgctgg ttcccggcgc attagtcatc
     2101 ggcctgctga tgctgttcct tgacctgaag caccagctct ttttctggag actttacacg
     2161 accgtccgtt ttgaatcccc gatgtcgtgg ggtgcctggg tgctgatggc cgttaccccg
     2221 ctgtcgttca tctgggcggc aacatacatt aaagagatca ggcccgagtg gaactggaag
     2281 ttcaatctcc tggagaaatt tgaagcattc gctgttaaaa accggaaacc gatggcatgg
     2341 attttcatcg tcctgtcagt agttctgggt atttataccg ggatcctgct ctctgccttt
     2401 aatgcgcgcc cgctctggaa taattccatc ctggggccgc ttttcctggt ctccggcctt
     2461 tccaccggta ccgccgtcat catgtggatc acaaaaaatc cccgggaacg catcattatg
     2521 agccggatcg acctggtcct tatcatcgtg gaacttttct ttatcacaca catgatgatg
     2581 gggatgctgg ccgggtcatc ggttcagatt gccgcggccg gcctgttcct tggcgggcag
     2641 ttcaccgtat ccttctgggt gtttgtgatc gtcctcggat tggtcttccc ggcggtactg
     2701 gaaatccttg aactggcggg cttcaaaatc ccggtagcta ttccagcctt gctgattctt
     2761 tttggcggcc tggtcttccg gtttattatg gtggaggccg gacaaattac ccgttatctt
     2821 tattaatctt aagatattta ttatatgatg gaaaataaca gaaaggaccg gagccagaac
     2881 tacctcaatc cctatttcgg cggggtactt ctcggcattg tcctcatcct cactttttat
     2941 ataaccggaa gaggattagg cgccagcggc gccgctaaaa gcacggtagt gaccacaatt
     3001 cagtcggtcg ctcccgaata tgcaaaaaag tcacactatg tttcacagtt tataagcgat
     3061 gataaccatc ccatgaacaa ctggctggtg ttcgaggtac tgggggtgtt cgccggcgct
     3121 ttcctgtcgg gaacgcttta cggccgcctg aaacccaggg tggaacattc cccgaaaatc
     3181 acctcgaaaa aacgccttgt ggtggcgctt ctcggaggcc tcctgtttgg tttcggctca
     3241 caggtagcca ggggatgtac cagcggcgca gccctgtcgg ggatggccgt actctctttc
     3301 gggggaatta ttaccatgat gggcattttt ggctcagcct acctttttag ctggatattt
     3361 aaaagactct ggatttaatt tgtaaaacaa tgggacctct tataccaaac gatattatac
     3421 cggccgactg gaacaatgcc atcgccctgt tattaggtat ggccttcggc ttcgtgctcg
     3481 aagcctccgg cttctcctcc tcccgaagga tcatggggac atttttcgga tacgattttg
     3541 tagtcctcag ggttttcttt accgcagccg tcacctccat gatcggtatt ctctacctcg
     3601 attatttcgg gtgggtcgat ttcggccagc tttacatcca tcctacgtat cttatgtcgg
     3661 cgatcatcgg cggtgtgatc atgggattcg ggtttgccct gggaggatat tgccccggga
     3721 caagctactg cgccgtcgcc atcggaaaac tcgatgccct ggcttactcc atcgggctgt
     3781 ttatcgggat ctatctcttt acctttgctt atccgctgct tgaaggattg tacaacggaa
     3841 gttttatggg gtatgttact gcaccggagg tcatggggat caatcagtac ctgtttgtat
     3901 ttttcgtcgt tatcgcagcc ctcggaatgt ttttcgtagc caccatcgta caacggaaag
     3961 tcaaaaaagt ggaatattaa tccgatatcc tatgtccaga aaatatatca tacccgttgc
     4021 attgctcctg atcctgagca tcgttctcgt tcttcttccg cgcaaacaag atcatagcca
     4081 aatgagcccg gaagaattgc tgcgacaaat taacagcacc tcgcgcttcg taagcaccga
     4141 cgaagtggcc gaccggctga tccgcaaaga tccttcactg gccctcattg atgtcaggaa
     4201 cgataagctg ttcagcgaat actcgctgcc gggcgctctg aatataccgg ttgataacat
     4261 cctggcagat gagagccaag aaatcttatc ccaggaagga ctggagtttg ttttctattc
     4321 aaccgatgac atcctggccg accagacctg gatcatcctg aaacgcaagg gattaaaaaa
     4381 tatttatgta ctgaaaggcg ggctgaatga atggttccgc acctttttca ccaatcctcc
     4441 cccggctgaa actgccgact cggaagagtt cgccctgtac cagttcagga tgggcgtgcg
     4501 gcagtatttc acgggcaccc ctgcggatag ccccgaaaag agcagcgctg aaaagatcat
     4561 cgttaccccg aaaaagaaaa aatcggccgc cgagggcggt tgctgaaact ctttaataga
     4621 atgccatgaa agaattgaac agaaccgacc ggctgaccat cgccaccacc atgctggcca
     4681 tcatactcat catcgggctg gtgaccatga gaaaaccggc tgtcgaattt gccattcctg
     4741 ccacagaggt cacgaagctg gctaccgacc cgtcgtatgt catcccagtg acgatactgg
     4801 ccgaaatgat gaaaaacaat gatccggccc tggcactgat cgatgtcagg aatcccgtaa
     4861 aattcaggca ggaacacatc ccttcagctt tgaacatccc ttctgccgat ctgctctcgg
     4921 aacagaacct gaatttgata aagaacctgg ctgaagagga taaaaaaatc atcatctacg
     4981 gcggcaatca cctggaggca aatggttcct ggctgatatt gaagcaattg ggcatcaaca
     5041 atctttatat cctggatgcc ggaatggaag gttacaacct atacaggcaa aatctttcgg
     5101 ttaatctccc ttttgaagat gtgaaatctg aaagaccggc ttacaattac aacgaaattt
     5161 tgaatagttt tgggacttcg tcttctgcac aggaaaccgg caagaccgaa ccggtcagga
     5221 tcactaaaaa agccaaaaag tctgctgccg aaggcgggtg ctaaaaatgc cggaagagat
     5281 atccaataaa aaagacaaag gaaaagcagc tgaaaaagag atgagcttct gggaacacct
     5341 ggaggaactc cggtggcacc tgctccgttc ggctgtagtc ctggtggttc tggcaatcgg
     5401 cgctttcctg atgcgccggt ttattttcga tgtgatcatc ctggcgccaa aagaacccgg
     5461 tttcatttct tacagggcct tgtgcacgat cggaaaatgg ctgcataccg attccctctg
     5521 cctcgatatc gaaccccttc agattgtcaa tatccagatg tcgggccagt tcatggtcca
     5581 tctttatatc tcgctgatga cggctgttgt cgtggctttg ccctatctgc tctgggaaat
     5641 atggtcgtt
//
LOCUS       DAOQXS010000139         5637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14627_length_5637_cov_3.543354, whole genome shotgun sequence.
ACCESSION   DAOQXS010000139 DAOQXS010000000
VERSION     DAOQXS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14627_length_5637_cov_3.543354"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKM34_05170"
     CDS             complement(<1..807)
                     /locus_tag="PKM34_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83013.1"
                     /translation="MKKINFLTMMLSVAVIVSLIVVSACTKEGPMGPAGKDGEDGING
                     TNGTDGTAVCGVCHNFSEDLTAKVAQYNKSQHGMGTTSFEGNRTSCAPCHTSKGFREA
                     IANDTTETLAPISNPTQPNCYTCHNIHQTYTESDWALATTDPVTFRFNAGNPGMQFDG
                     GNGNLCVNCHQSRTINPYPDINNLTGTVTVGNFRYGPHYGPQSQILSGQAGFNVGITG
                     STSHKHYEWIENTCTDCHMAEPFGQLAGGHNMSVYIEEEGDYNWAGCTSCH"
     gene            complement(1072..1767)
                     /locus_tag="PKM34_05175"
     CDS             complement(1072..1767)
                     /locus_tag="PKM34_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ83014.1"
                     /translation="MAKIFRETSCNDCRKRIEIFKVLRDEDFKKIEEKRYEVLYHTGE
                     IIFKQGTSFTHTICILEGLVKVYLESGNRKNFILALIGPGEMIGSPGMYSDDKHYFSV
                     AALEDTIACYVEREIIEDIIQSNSRFAIEMLKRSNFRDIRHFQKFRTLTQKQMPGRVA
                     EAILYLSKAVYKSNPCRLSVSRQDIADMAAVTKESTIRVLKEFKDAGHIAVSGNTLEI
                     LNEKALVNISENG"
     gene            1879..3621
                     /locus_tag="PKM34_05180"
     CDS             1879..3621
                     /locus_tag="PKM34_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ83015.1"
                     /translation="MKQKPRLSFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGARV
                     EDIPILWIAAPVTGLIIQPIIGHMSDNTWNRLGRRRPYFLTGAILASLALLFMPNSPV
                     LWIAAGMLWIMDASINISMEPFRAFVGDMLPSRQRTMGFAMQSFFIGTGAVVASALPY
                     IMTNYLGIENTAPKGVIPPSVKLSFYIGAAVFFLAVLYTILRTKEYSPQQMKEFAGED
                     AAGGESASHSDDILPSKTFINKSYIWIFSGVVTTFLVYWFKLQQELYILTVGLLAFGI
                     IQLLTGLLVGKGKDKNGFVVVINDLFKMPVTMKQLAVVQFFSWFALFAMWIYTTAAVT
                     STKFDMKLDRDLYDQLQTTLSHVPSGSGTSDEAETKAVKAELDHLGEKLEKGRDVAIT
                     MKVVQFFNDETRAGQLNLGPAVTDRLNHINKEYNKGADWVGILMAVYNGFAAIMAFVI
                     IALAGKIGRKRTHAISLLVGGLSLASFFLIRNPNLLIISELGIGLAWASILAMPYAIL
                     TGSLPQHKLGTYMGIFNFFIVIPQILAASILGFFVKTIAGGEAIYALILGGISMVIAA
                     GLVLFVRDEDEKLR"
     gene            complement(3613..4245)
                     /locus_tag="PKM34_05185"
     CDS             complement(3613..4245)
                     /locus_tag="PKM34_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83016.1"
                     /translation="MLGDVLLIEDKHRIAGEAIIQKILEQKKEKFMIAISGESGSGKS
                     ELAHVIAKGLRKHGIMAKPVHIDNYYRIHPLERTEWRKKNGIENVVGYGEYDWDTIYR
                     NIEDFKNDRKSEMPCVDLVTEQVDRLTTDFKGIDMLVIDGLYAIKTEGVDLRIFIELT
                     YHETKKAQVVRGKEPQNEYRMAVLEQEHRMVQALKPTADILISKAYEVLS"
     gene            complement(4368..>5637)
                     /locus_tag="PKM34_05190"
     CDS             complement(4368..>5637)
                     /locus_tag="PKM34_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83017.1"
                     /translation="LYRHKRLDEARKYALEYGYQGAMFPWQSGSSGREETQILHLNPL
                     SGEWGPDHSSLQRHVSLAIAYNTWQYYHITGDLAFMEEYGAELIFEICRFWAGKSKLN
                     RVTGRYEIIKVMGPDEFHEKYADTDEGGLKDNAYTNMMVAWLFGRAAVLFDRMSPQAK
                     ETVMAKIHLATGEIERWNDIAARLNLVISPEGIIAQYDGYFDLLELDFEEYRKKYKNI
                     YRMDRLLKAEGKSPDEYKVAKQADTLMTFYNLSGEEVGEILEKLSYKLPDDYLMRNLN
                     YYLARTSHGSTLSRVVHAQLANMTGNKSLAWELYQDALTSDYADIQGGTTAEGIHAGV
                     MTGTVWVALVSFAGLNLHGDYPRFNPQLPAHWRKISFFFSFRDGEYECEVTAAGIRIR
                     VDKPEPGSARIGFQDKTFEINTGEWVDLEI"
BASE COUNT         1291 a         1374 c         1350 g         1622 t
ORIGIN      
        1 atggcaagag gtgcaaccgg cccagttgta gtcaccttct tcttcgatat aaacactcat
       61 attatgtcct ccggccagtt gtccgaacgg ttcggccata tggcaatcgg tacaggtgtt
      121 ctcgatccac tcataatgtt tatgagacgt tgacccggtg atgccgacat tgaaaccggc
      181 ctggcctgaa aggatctgcg actgcgggcc gtagtgaggg ccataacgga aattccccac
      241 ggtaaccgtt ccggtcaggt tgttaatgtc cggatacgga ttgatggtac gtgactggtg
      301 acagtttaca cagaggttgc cgtttccacc gtcgaattgc atccccggat taccggcgtt
      361 gaacctgaag gtgacagggt cagtggtagc cagtgcccag tcggattcgg tgtaggtttg
      421 gtggatgttg tgacaggtgt agcagtttgg ctgtgtcgga ttcgaaatcg gagccaatgt
      481 ttctgtggta tcgttggcaa tggcttcccg gaatcctttg cttgtgtggc aaggcgcaca
      541 actggtgcgg tttccttcaa aagaggtggt gcccataccg tgctgtgatt tgttgtactg
      601 agctaccttg gccgtcaggt cttcgctgaa attgtggcat acgccgcaca ctgcggttcc
      661 gtcggtaccg ttggtgccat tgataccatc ttctccgtct ttacctgcag gtcccatcgg
      721 gccttctttt gtacaggcac tgacaacaat cagcgagacg attaccgcca ctgataacat
      781 catggttaag aaattgattt ttttcatatt gcgcgttgtt taagttataa tttcctgtga
      841 gcaatattac tcacattcaa taatgtttat atataaaagg gtggtgatat ttacagttat
      901 taatgtatat cgaaatcaac aaatgattga tatatatcaa tgatacatta tatatttatt
      961 aatattaacg ttgtaaatat catgatgaga gatacataaa tagaataaca atatgatgta
     1021 tatgaatgat attttttatt tagaattgat ataaatttat agatccggtt ttcaaccgtt
     1081 ttcagagata ttcacaaggg ccttttcgtt gaggatttcc agggtgtttc cggaaacagc
     1141 aatgtgtcct gcgtctttga attcctttaa aacccggatg gtgctttctt tggtcacggc
     1201 agccatatcg gcgatatcct gccgtgaaac ggataaccgg caaggattgg atttataaac
     1261 ggcctttgaa agataaagaa tggcttctgc gacccttccg ggcatttgtt tttgcgtcag
     1321 tgtccggaat ttttgaaaat gccggatatc cctgaaattg gaacgcttca gcatttctat
     1381 ggcaaagcgg ctgtttgact ggatgatatc ttcgattatt tccctctcca cgtaacaggc
     1441 gatggtatct tccagggcgg ctacagaaaa atagtgttta tcgtcggaat acattcccgg
     1501 gctgccgatc atctctccgg ggccaatcag tgccaggatg aaatttttcc ggttcccgga
     1561 ttcaaggtac actttaacga gcccctccaa tatacatatg gtatgggtaa aggatgtgcc
     1621 ttgtttaaaa atgatctctc cggtatgata caatacttca tacctttttt cttctatctt
     1681 tttaaaatcc tcatcccgca gcaccttaaa aatttcgatg cgtttgcggc agtcgttgca
     1741 actggtttcc cggaagattt tagccataat attatttttc aatgatctga aggtcaaata
     1801 taatgatatt tttttgaaaa tgagatggag tctcatctta ttaaaattat ccttatcatt
     1861 gcttaataaa aaaaaattat gaaacagaaa ccccggttat ccttctggca gatatggaac
     1921 atgagttttg gttttctcgg tatccagttt ggatttgctt tgcagaatgc caatgtcagc
     1981 cgaatttttg aaaccctcgg ggccagggtg gaggacattc cgatcctgtg gatcgccgcc
     2041 ccggttaccg gccttatcat ccagccgatc atcgggcata tgagcgataa tacctggaac
     2101 cggctgggcc gccgccggcc ctattttctg accggtgcca tcctggcttc actggcgttg
     2161 ctgttcatgc ccaactcacc tgtcctctgg atagctgcag gaatgctctg gatcatggac
     2221 gcctccatca atatctccat ggagccgttc cgggcttttg tcggcgacat gctgccctcc
     2281 cggcagagaa ccatgggttt tgccatgcag agtttcttta tcggtacggg agccgtggtg
     2341 gcatcggcct tgccgtacat catgaccaat tacctgggca ttgaaaacac cgctcccaaa
     2401 ggggtcatcc cgccatcggt caaactatcg ttttacatcg gtgccgctgt atttttcctg
     2461 gcagttttat ataccatatt aagaactaag gagtattctc cgcaacagat gaaggaattt
     2521 gccggcgaag atgcggcagg cggcgaatct gcttctcatt ctgatgatat actgccgtcg
     2581 aaaacattta ttaataaaag ctatatctgg atcttttccg gcgtagtcac cactttcctg
     2641 gtctattggt ttaagctgca acaggaactg tatatcctga ctgtcgggct gctggctttc
     2701 ggtatcattc aattgcttac aggcttgctg gttggaaagg gaaaagataa aaacgggttt
     2761 gttgtcgtta taaacgattt gttcaaaatg cccgtgacga tgaaacagct tgcggttgtt
     2821 cagttctttt cctggttcgc cttgtttgcc atgtggatct atactaccgc cgccgtcacc
     2881 agcaccaagt ttgatatgaa gctcgaccgc gatctctatg atcagcttca gaccacgctc
     2941 agtcatgttc cctccgggtc aggtacttcc gatgaggcag aaaccaaagc ggtgaaagcg
     3001 gagcttgatc atctgggaga aaaactggaa aagggcaggg atgtcgcgat taccatgaag
     3061 gtggtgcaat ttttcaatga tgaaacgcgc gccgggcaat tgaacctggg gccggccgtg
     3121 accgaccgcc tgaaccatat taacaaggaa tacaacaaag gcgccgactg ggtaggtatc
     3181 ctgatggccg tttacaacgg ttttgcggcg atcatggcgt ttgtgatcat tgccctggcc
     3241 ggcaagatag ggcggaagcg gacccatgcc atcagccttc tggtcggagg gctcagcctt
     3301 gcatcgttct tcctcatccg caacccgaac ctgctgatca tctcagagct ggggatcggc
     3361 ctcgcctggg ccagcatcct ggccatgccc tatgccatac tgaccggctc gttaccccaa
     3421 cataagctgg ggacatatat gggcattttt aatttcttta ttgtgatccc gcaaatcctg
     3481 gcggccagca tccttggatt ttttgtcaaa accattgccg gcggggaagc tatttacgcc
     3541 ctgatcctcg gcggcatttc catggtcatc gctgcagggc ttgtattatt tgtcagggat
     3601 gaggatgaga aattacgata aaacctcgta ggctttcgag atcaggatat cggcagtcgg
     3661 tttcagtgcc tggaccatcc ggtgttcctg ttccaggacc gccatccggt attcgttctg
     3721 cggttctttg cccctgacca cctgtgcttt cttggtttca tggtaggtca gctcaataaa
     3781 aatcctcagg tcgacacctt cggttttaat ggcatacaat ccgtcgatca ccagcatatc
     3841 aatccctttg aaatcggttg taagccggtc gacttgctcc gtaaccaaat caacgcaggg
     3901 catttccgat ttccggtcgt ttttgaaatc ttcgatattc cggtaaatgg tatcccaatc
     3961 atactcaccg tatcctacca cattctcgat cccgttcttt tttctccatt cagtcctctc
     4021 caatgggtgt atccggtaat aattatcgat atggaccggt ttggccataa tgccgtgctt
     4081 tcggagccct ttggcaataa catgggccag ttccgattta cccgatcccg attcgcccga
     4141 gatggctatc atgaatttct cttttttttg ttccaggatt ttctggatga ttgcttcgcc
     4201 ggcaatccgg tgtttatctt caataagcaa aacgtctcct aacataccgt cattattagt
     4261 tcaaagttta aagttccaag ttccaggttc caggttccag gttccagggt tccaaagttc
     4321 cagggttcca aattttcaaa ttttcaaatt tccaaatttt caaattttca aatctccagg
     4381 tcgacccatt cgccggtgtt gatttcaaat gttttatcct gaaatccgat ccgtgcagac
     4441 ccgggttcgg gtttatcaac ccgtatgcga ataccggcag cggtcacctc acattcgtac
     4501 tcgccgtcgc ggaagctgaa gaaaaagctt atcttcctcc agtgggccgg cagttgcggg
     4561 ttgaaacgcg ggtaatctcc atgcaggttc aatccggcga aggagaccaa tgctacccaa
     4621 accgttccgg tcataacgcc ggcgtggata ccttcggcag tcgttccgcc ctggatatcg
     4681 gcataatcgc tggtaagtgc gtcctggtaa agttcccagg ccaggctttt gttgcccgtc
     4741 atgttggcca gttgggcatg gactacccgg ctgagggtgg aaccgtggga ggtccgggcc
     4801 aggtagtagt ttagattgcg catcaggtaa tcatccggta gtttatagct cagcttttcg
     4861 aggatttcac cgacctcttc accgcttagg ttgtagaaag tcatcagcgt atcggcctgt
     4921 ttggccacct tgtattcgtc gggcgatttg ccttcggctt tcagcagccg gtccatccgg
     4981 tagatgtttt tatatttctt gcgatactct tcaaaatcaa gttccagcag gtcgaaataa
     5041 ccgtcatact gtgcgatgat cccttcggga gagatcacca ggttcagcct ggcggcaata
     5101 tcgttccatc gctcaatctc cccggtagcg agatgaatct ttgccatgac cgtttccttt
     5161 gcctgcgggc tcatccggtc gaacaacacg gcagctctgc cgaacagcca tgcaaccatc
     5221 atattggtgt aagcgttatc tttcagccct ccttcatcgg tatcggcata cttttcatga
     5281 aactcatccg gtcccatgac cttaatgatc tcataacggc cggttacgcg gttcagcttg
     5341 cttttccccg cccagaaacg gcagatctca aatatcagtt cggcgccgta ttcttccata
     5401 aatgccaggt cgccggtgat atggtagtat tgccaggtat tataggcaat agccagcgaa
     5461 acatggcgct gaagcgagct gtggtcgggc ccccactccc cggaaagagg gttaaggtgg
     5521 aggatttgcg tttcctcgcg tccgctgctg ccgctctgcc aggggaacat agcgccctgg
     5581 tatccatact ccagggcata tttcctggct tcgtccagcc ttttatgccg gtacagg
//
LOCUS       DAOQXS010000140         5634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14633_length_5634_cov_3.584155, whole genome shotgun sequence.
ACCESSION   DAOQXS010000140 DAOQXS010000000
VERSION     DAOQXS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14633_length_5634_cov_3.584155"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1574
                     /locus_tag="PKM34_05195"
     CDS             117..1574
                     /locus_tag="PKM34_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNQ83018.1"
                     /translation="MKRRVFSSVFVFAIIYTIFFQITALAGNDPSSGEPRAKYVFLFI
                     GDGMGQAQVNLAQAYLAALDGRIGLDDLSFTRFPEAGFCNTYAHNRLITCSAAAGTAL
                     ATGNKTNINRISMDPEATMNLTSVAAKAKNKGYKVGILTSVSIDHATPAVFYAHQPDR
                     DLYFDIGRELTQSRFDLFAGGGFVQPDSTNGGQTVNLVSLAAENGFNVADTREEFEKL
                     APGTGKTLILSPRIAGEASMPYYIDMDPGDITLADYTAKAIDLLSGDEGFFMMVEGGK
                     IDWACHANDASTVIQEIIIFSRAIESAVRFYEKHPEETLIIVTADHETGGLALGNRKM
                     KYSSNLELLKYQLSSTDEMNKIVSQFRVNKSGDPDADFLRMMKVVENETGLHSRANGT
                     LLTDSELARLKERFLESVYAAGPEQGTNIPEDKFIESVIGLRDEKAGISWGSGSHTFV
                     SVPVYAIGQGAEKFSGYIDNTDIPKLIGEIMQLER"
     gene            complement(1586..2608)
                     /gene="pheS"
                     /locus_tag="PKM34_05200"
     CDS             complement(1586..2608)
                     /gene="pheS"
                     /locus_tag="PKM34_05200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ83019.1"
                     /translation="MQEKISSYLAEIDRFTTTNPEELEQFRIKFISKKGLIPALFEDF
                     KNVAPEMRREMGQALNELKNKAQEKINLLREQIEASATEVKRGTDLTLPVDFARNGTR
                     HPLSITRNEIVRIFSRIGFVVAEGPEVEDDWHNFTALNFQQDHPARDMQDTFFIERNP
                     DIVLRTHTSSVQIRAMENRKPPLREIYPGRVFRNEAISARAHCIFHQVEGLYVDHDVS
                     FADLKQTLYYFAHEMFGEGTEVRFRPSYFPFTEPSAEMDVTCFVCGGKGCPICKYSGW
                     VEILGCGMVDPNVLENCGIDSREYTGFAFGMGIERITILKYRIQDIRLFFENDVRFLR
                     QFAGVV"
     gene            complement(2658..3332)
                     /locus_tag="PKM34_05205"
     CDS             complement(2658..3332)
                     /locus_tag="PKM34_05205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ83020.1"
                     /translation="MKKTIIILTALFMALQQSGCSQSPKQEKGKEPVKGNGPETLVLI
                     KTSMGDITVKLYNDTPKHRDNFVKLIREGWYNDSPFHRIIKGFMIQGGGNKDGRQDPG
                     YTIPAEILPNHFHKKGVLAAARMGDNVNPTKASSGCQFYIVEGNIVNEETLNSFEMRY
                     KTKYNAEQRQAYSTVGGAPWLDGGYTVFGEVVSGIEVVDRIAAVPTGPGDKPVAPVTM
                     SIEILE"
     gene            complement(3389..3973)
                     /locus_tag="PKM34_05210"
     CDS             complement(3389..3973)
                     /locus_tag="PKM34_05210"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNQ83021.1"
                     /translation="MFLERSNGNMRSGWIEVICGSMFSGKTEELIRRLKRALIARQKV
                     AIYKPAVDIRFSTNDVVSHDENSIPSTPVEASTQILLYANDYDVVGIDEAQFFDDELV
                     NVCNKLAAMGIRVIVAGLDMDYTGKPFGPIPALLATAEYVDKVHAICISCGGLAHYSH
                     RTSNQEGLVVLGATDRYEPLCRKCYLEKIAAPKS"
     gene            complement(4096..4434)
                     /locus_tag="PKM34_05215"
     CDS             complement(4096..4434)
                     /locus_tag="PKM34_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83022.1"
                     /translation="MKTLKSILVATLVAVTMVGLSNTDGFKIKPKPLKVVNVSFEKAI
                     QCPGLVKAMYEQIDKEELLSNGTLNKYVAEVTYSGTLYRITGTRAQWIWFFRIKIDLP
                     YNDDPGIHSN"
     gene            4694..5593
                     /locus_tag="PKM34_05220"
     CDS             4694..5593
                     /locus_tag="PKM34_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83023.1"
                     /translation="MDKKIIKGLIDDKLEVIQEQFSIIRAYEGKIPVIELDLILGSIR
                     DLYELVLGLQQENMAGTKPEPVIIPEEEQPEEPIIEVTLPTPPEPKPEPSRGTETAGR
                     FASGILSMEPVREESPKPELIRQAPPAYIRPTQHYADPVAVETKVAPDPEPEPFINKE
                     QTTEAYPPEMPPVADSPSASEDKPAADNFQRTTFDLFSDVPPVTIADRLREGQDKRLA
                     DKLQEKITDLRSAIGINDKFLFINELFDGNMRVYDDIVQKLSSAATIAQIDLLLLDLK
                     IAYNWDSESPTVKKFVDLVRRRF"
BASE COUNT         1426 a         1373 c         1413 g         1422 t
ORIGIN      
        1 gaatcttctt gccacgaatg cacgaatgta tttgccacta aaacactaaa gcaccaaagg
       61 gcataaaagg gaagcggtta aggtttattt ttacaacgta aaatgaaatc agttttatga
      121 aaagaagagt ttttagcagc gtttttgtct tcgctatcat ttatacgata ttttttcaga
      181 ttactgcctt agccggcaat gatccctcat cgggcgaacc gagggcgaaa tatgtctttt
      241 tgttcatcgg cgacgggatg gggcaggccc aggtcaacct ggcacaggct tacctggctg
      301 cgctggatgg caggatcggc cttgatgatc tttcgtttac gcggtttccc gaggccggat
      361 tttgcaatac gtatgcccat aaccggctga tcacctgttc ggcggcagca ggaacagctt
      421 tggccaccgg caataaaacc aatattaacc ggatttccat ggatccggag gcgaccatga
      481 acctgacatc tgttgctgcc aaagcgaaga acaaaggcta caaagtgggg attctcacca
      541 gtgtgtcgat cgatcatgct acgccggccg ttttttatgc ccatcagccc gaccgtgatc
      601 tctatttcga tatcggccgc gaactgacgc agagccgttt cgatttgttt gccggcggcg
      661 gttttgttca acccgacagc acgaacgggg gtcaaaccgt caacctggtc agcctggcag
      721 cggaaaacgg gtttaatgta gctgatacgc gggaagaatt tgagaaactg gctccgggaa
      781 cggggaaaac cctgatcctg tcgccgcgga ttgccggcga agcttcgatg ccgtattata
      841 tcgatatgga tcccggagat atcacgctgg ccgattatac cgcaaaagcg atcgatcttc
      901 ttagcggtga tgaaggattt ttcatgatgg tggaaggagg aaagatcgac tgggcctgtc
      961 atgccaatga tgcctccacg gtaatccagg agatcatcat tttcagccgg gccattgaaa
     1021 gcgctgtccg tttttacgaa aagcaccccg aggaaacgct gatcattgtg acggccgacc
     1081 acgaaacggg tggtctggcc ctcggtaacc ggaaaatgaa atattcatcc aacctggaac
     1141 ttctcaaata ccagttgtcg tccactgacg aaatgaataa aatcgtttcg cagttcaggg
     1201 tcaacaaatc gggtgatccg gatgcagatt ttttacggat gatgaaggtg gtggaaaatg
     1261 agaccgggtt gcacagccgg gcgaatggta cgctgcttac cgacagtgaa ctggcccgtt
     1321 tgaaagagag gtttcttgaa agtgtgtatg ctgccggccc ggagcaggga accaatatcc
     1381 ccgaagataa attcatcgag tcggtcatcg gtttgcgcga cgagaaagca ggaatttcat
     1441 ggggaagcgg ttcacacact ttcgtcagcg tgccggtata tgccatcggg caaggcgctg
     1501 agaagttctc agggtatatc gataacaccg atattccgaa actgatcggt gagatcatgc
     1561 agctggaaag atagaggaat agtgatcaga ccactccggc aaactgccgc aggaaacgca
     1621 catcattctc gaagaacagg cgaatatcct ggattcggta tttcaatatc gtgatccgtt
     1681 ctatccccat tccgaaggcg aagccggtgt attcccggct gtcgatgcca caattctcaa
     1741 ggacattggg atcgaccatt ccgcaaccca ggatctccac ccagccggag tatttgcaga
     1801 tcgggcagcc cttgcctcca caaacaaagc aggtgacatc catttcggcc gaaggctcgg
     1861 tgaacgggaa gtagctgggc cggaaacgga cctctgtccc ttcaccgaac atctcatggg
     1921 caaagtagta aagggtttgt ttaagatcgg caaacgacac atcgtgatcg acatacagcc
     1981 cttctacctg gtgaaagatg cagtgggcgc gggccgagat ggcttcattg cggaaaacgc
     2041 gccccgggta tatttcccga agcggtggtt tacggttttc catagccctg atctgaacgg
     2101 aagaggtatg ggtccgcaga acgatatcag gatttcgttc gataaaaaag gtatcctgca
     2161 tatcgcgggc cggatgatcc tgctgaaaat tcagcgccgt aaaattatgc cagtcgtctt
     2221 ccacctcggg cccttccgcc accacgaacc cgatgcggga gaagatccgg acgatttcgt
     2281 ttcgggtgat agagagcggg tgcctggtac cgtttctggc aaaatcgacg ggcagggtaa
     2341 ggtcggtgcc ccgtttcact tccgttgcag aggcctcgat ctgttccctg agaagattga
     2401 tcttctcctg ggctttgttt ttcagttcat tcagcgcctg ccccatttcc cgcctcatct
     2461 ccggggcgac atttttaaaa tcttcgaaaa gcgccgggat caatcctttt ttgctgataa
     2521 acttgatacg gaattgctcc agctcctccg ggtttgtcgt cgtgaaacgg tctatctctg
     2581 cgagatagga agatattttt tcctgcatga aagtatgggc tgcggatggc gtcccgtggg
     2641 acgccatcct gattaaatta ctccaggatt tctatcgaca tggtcaccgg cgccacgggt
     2701 ttgtcgcccg ggccggtcgg aacggcagcg atcctgtcaa ccacttcaat tccggaaacg
     2761 acttcaccga aaactgtgta cccgccgtcg agccagggtg cgccgccaac ggtgctgtag
     2821 gcctgcctct gttcggcgtt gtatttggtc ttataccgca tttcgaagga gttaagggtt
     2881 tcttcattga caatatttcc ctcgacaata tagaactggc agcccgaaga agctttggtc
     2941 gggttcacgt tatcgcccat tcgtgcagca gccagaacac cttttttatg aaaatgattc
     3001 ggcagaatct cagccggaat ggtatatccc ggatcctggc ggccgtcttt attgcctccc
     3061 ccctggatca tgaaaccttt gatgatccga tggaacggag aatcattgta ccatccttca
     3121 cggatcagtt tcacgaagtt atccctgtgt ttcggggtat cattatatag tttgacggtg
     3181 atgtcgccca tgctggtttt aataagcaca agtgtttcag gaccattacc tttgaccggt
     3241 tctttccctt tttcctgttt agggctctgt gagcagcccg attgctgtaa tgccatgaat
     3301 aatgcagtta aaatgattat tgttttcttc attggtaaag ctttaaaatg attaagcgcc
     3361 gcaaaattaa aaaattagag acagattttc aggatttcgg cgcagctatt ttttccagat
     3421 agcattttct gcaaagaggt tcatagcggt cggtggcccc gagtactacc aggccttcct
     3481 ggttgctggt ccggtgcgaa tagtgggcca atccgccgca actgatgcag atggcgtgaa
     3541 ctttgtccac atattcggct gtggccagaa gtgcgggaat cgggccgaaa ggcttgccgg
     3601 tgtaatccat atccaggcct gccacgatca cacggatccc catcgcggcc agtttattac
     3661 agacattcac cagctcgtcg tcgaaaaact gggcttcatc gattcccacc acgtcataat
     3721 cgttggcata gagcaaaatc tgcgtggaag cttccaccgg tgtagatggt atggaatttt
     3781 catcgtgtga aacaacatca tttgtgctga aacggatatc cacggcaggt ttgtaaatcg
     3841 ccaccttttg cctggctatc agggcccgtt tgagccggcg gatcaactct tccgtcttgc
     3901 ccgaaaacat cgatccgcag atcacctcga tccagcctga ccgcatatta ccgttggaac
     3961 gttctaaaaa caaattgaat gaagaattat ataaagtccg taatgatttt gtatttttca
     4021 aaattataaa aaatagcggt taaaaagaaa gggcacccaa cagggcaccc ttcttcttcg
     4081 ctttccgatc tccggtcagt tggagtgtat tcccggatca tcgttgtagg gcaggtcgat
     4141 tttgatcctg aagaaccaga tccactgagc gcgtgtgccg gtgatcctgt aaagggtgcc
     4201 gctgtatgtc acttctgcga cgtacttgtt cagcgttccg ttgctcagca gctcttcctt
     4261 gtcgatttgc tcgtacatgg ctttcaccag acccgggcac tgaatcgctt tttcgaacga
     4321 cacattgacg actttgagcg gtttgggttt gatcttgaat ccatcggtgt tggaaagtcc
     4381 gaccatcgtc acagcaacca aagttgcaac cagaattgat tttaacgttt tcataataac
     4441 ctccgttttt taaagttagt tttaaggaat tttaaagagc ttaatttcaa tgttgatgaa
     4501 acaaaggtat gccggcctta tatcctgaac atggcagaaa aaggcgatct ttttttttag
     4561 tggaaaacaa tgagtatgca gttcaggcca tggtaatgaa ggatataatg atgaatttgt
     4621 catcatcttg aattaatctt cttaccttta ttgtcggaat tacaaagcat ttgcagataa
     4681 tacgaccggt aatatggata aaaaaatcat taaaggcctt atcgatgata aactggaggt
     4741 tatccaggag caattctcga ttatccgggc ctatgaaggc aagatccccg ttattgaact
     4801 ggacctgata ctgggcagta tccgcgacct gtatgagctc gtgctgggtt tgcaacagga
     4861 aaacatggca ggaacgaagc ctgaaccggt catcataccg gaggaagaac agcctgaaga
     4921 acccattatt gaagtgacgt taccaactcc accggaacca aaaccggaac cttcaagagg
     4981 aacagaaacg gccggacgat tcgcttcggg aatactcagc atggaacctg tcagggaaga
     5041 atctcccaaa cccgaactta tccggcaggc gccccccgct tacatccgtc cgacacagca
     5101 ttatgccgat cctgtcgctg tagaaacaaa agttgcgccc gatccggagc ctgagccttt
     5161 tatcaacaaa gagcagacaa ctgaggccta tccgccggag atgccgccgg tagctgattc
     5221 gccttctgct tccgaagata agcctgcagc agacaatttc cagcggacca cattcgatct
     5281 ttttagcgat gtcccgccgg tgaccattgc tgaccgtctt cgggaagggc aggacaaacg
     5341 gctggccgat aagttgcagg agaaaatcac cgatctgcgt tctgccattg gcatcaacga
     5401 caaattcctt tttataaatg agctgttcga tggcaacatg cgggtttacg acgatatcgt
     5461 ccagaaattg agcagcgcag cgacgatagc acagatagac ctgcttctcc tcgacttgaa
     5521 aatcgcttat aactgggaca gcgaatcacc caccgtcaag aaattcgtcg acctggtcag
     5581 gcggagattc tgatccttgt aacgaattta atgtctggca acatggcaaa gctt
//
LOCUS       DAOQXS010000141         5577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14836_length_5577_cov_4.424303, whole genome shotgun sequence.
ACCESSION   DAOQXS010000141 DAOQXS010000000
VERSION     DAOQXS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14836_length_5577_cov_4.424303"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKM34_05225"
     CDS             complement(<1..1439)
                     /locus_tag="PKM34_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ83024.1"
                     /translation="MNPLKKLAGQTLVYGMGTIVPRLLNYFLLTPFYTRIFITGEYGV
                     ITELYAYMAFLLVLLTYGMETAFFRFAGKEPDPKKVFSTSLVSLFVTSVLFVFLVVIF
                     SQPVATLIEYGNNPEYITMFSVIVALDAFTAIPFAYLRHRNKALRFSIIKIVNVLVNI
                     GLNFFFLWLAPKMLSANPGSWVRLVYDPAIGVGYAFIANLASSGLTLLLLLPDIFSVR
                     PAVDKDLLKRMLTYAFPLLIVGLAGMVNEVADKIIFKFLLIVPPGEADPKAYAMSELG
                     VYGAAYKLSVLMTLFIQMFRYAAEPFFFAQKKEENAKKIYADVMKYFVIFCLIIFLGV
                     TLFADIVKYFIGPDYREGLFILPIVLMANLLLGITFNLSIWYKLNDMTRFGAYIGLSG
                     AAMTILMNVLLVPRLSYLGAAIGHLSAYSLMVILSYYWGQKYYRIGYPMKRIGFYTIL
                     TLALFLPGYYLPVGNQALSLAINAWFPTG"
     gene            complement(1454..2494)
                     /locus_tag="PKM34_05230"
     CDS             complement(1454..2494)
                     /locus_tag="PKM34_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83025.1"
                     /translation="MKYLNPILFFVLFPFLATVAQPEIPAWCDKSFRESHYPADQYLT
                     GYMEGGKIVNETLDQARERMKKLAQSELIEGVMVQIQSYTESKVQDITRQSTSSVVQF
                     YEASVKTESNMELTGLTNEVFYNPETATVYAFSYANRYEIIGYYKANIGFYQQQIIQV
                     VELSDRLYTDGEKVKARSELEKAIPLFARIDYSYGVLLALKGNIDSLQYANSMQMKST
                     AIRKLADLEKGVIVFVQSSEDIFGSPSEILANALKRDLKGCSFTADESASDWKVTIDA
                     KAREYSYTNGFYFSYADVVLSLMKTRTGQLLYQEEFSQKGGGSDYRKAARKAMEEAAT
                     AVSGKIVAEINK"
     gene            complement(2564..3820)
                     /locus_tag="PKM34_05235"
     CDS             complement(2564..3820)
                     /locus_tag="PKM34_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNQ83026.1"
                     /translation="MKKSFIAVFILAGAIISGHSQSFTAYMLVAPEAYLEDVHKISIL
                     DFDGRMGTAMTDYLAAELMQEFRGTGGKSGGNYFSAGKSINNFQKGARTNVFSLVERS
                     ELDKVLREQNISNSGLIDDSQAAQIGKLLGIDAMITGSVAYSHQDEQKTLTYTDLNGN
                     KKYSYCTTRTVTAEGHLKIINVNTGQIIGTTVKTTSYSDKKCDDKRSTLFSVEELAKV
                     CYDGLATKLANYFTPVFLPMEYEFEKIKDKEYKEKGKLAMDYLESGDLNNAFSIYKSI
                     YDIDPYNSAASSNIARLYRIAGNYEKAREYYALAAEVDEVTYAPLVQAAIREKEMLSL
                     FEGYGINIEQTDFIQKSDALAERVTTRGNKSDKYDVRVNPDQTSDVVAKVPGSTEFTV
                     IEKQGEYYLIKLLGGKQGYIHDDYVK"
     gene            4123..4488
                     /locus_tag="PKM34_05240"
     CDS             4123..4488
                     /locus_tag="PKM34_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83027.1"
                     /translation="MPIKYVFSVEDELLKVTAHGKDDSFEEVMNYSRTIIEASRQHGC
                     TKILCDERHLQHAITATDTYLLAENASQHASYFSKVAIICHKRSLKEGKFYETVASNR
                     GLKILVTTDPTAALGWLDK"
     gene            4573..>5577
                     /locus_tag="PKM34_05245"
     CDS             4573..>5577
                     /locus_tag="PKM34_05245"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNQ83028.1"
                     /translation="MKMTKLFYLLPGLPALFLIIAPGFGCNKDDDNMPDATPVDYSLV
                     EHWLSVPAPEKEVDIFYLYPTSWQKLNPDDPNICDIDNPLMQAGSASAYHRQATAFAT
                     TGNVFAPYYRQADAQYTLSLPEAQRDSVIGGIPLLDATAAFDYYINHFNDGRPFILAG
                     HSQGSNILLFLLSGYLKQHPEVYSRMIAAYVIGYPVTQAYLTANPHLKFAERADDTGV
                     IISYNTQSPAVSPGQNPVVSDIVGLVINPISWTRDETPATTAEGLGSYMPDQEGVFAQ
                     VPQYADARIDTGQGVLICSTAVDSTLYLGFGPGVYHSYDYPFYYFNLRANAGLRAGKF
                     L"
BASE COUNT         1411 a         1402 c         1316 g         1448 t
ORIGIN      
        1 ctccctgtcg ggaaccaggc gttgatcgcc agcgacagcg cctggttccc gacagggagg
       61 tagtaaccgg gaaggaataa cgccagggtc agaattgtgt aaaacccgat ccgcttcatc
      121 gggtaaccga tccggtagta tttctgcccc cagtaatagg atagtatgac cataagactg
      181 taggcggaaa gatgacctat tgctgcaccg agataactca ggcggggtac cagcagcaca
      241 ttcatcagta tcgtcatggc ggcgcccgac aggccgatat aggcgccgaa tcgggtcatg
      301 tcgttgagct tgtaccagat cgacaggttg aaggtgatcc ccagcagaag gttagccatc
      361 agtacgatgg gcaggatgaa cagcccttcc cggtagtcgg gcccgatgaa gtatttgacg
      421 atatcggcaa acagggtcac acccaggaag atgatcaggc agaaaatgac gaaatacttc
      481 atcacatcgg cgtagatctt tttggcattc tcctccttct tctgggcgaa aaagaagggc
      541 tcggcggcat agcggaacat ctggataaac agcgtcatca gcaccgaaag cttgtaggcc
      601 gccccgtaaa cgcccagttc gctcatggca taggccttcg gatcagcctc gccgggcgga
      661 acgatgagga ggaacttgaa gatgatcttg tcggccactt cgttgaccat cccggccagc
      721 ccgacgatca gcagcgggaa agcgtaggtc agcatccgtt ttaaaaggtc tttgtcgaca
      781 gccggcctga cactgaaaat atcgggcagc aacaatagca gggtgagccc gcttgaggcc
      841 aggttggcga taaacgcgta ccctacgccg atggcagggt cgtacaccag ccgcacccag
      901 gagcccgggt tggcgctgag catcttcggc gccagccaga ggaaaaagaa gttcaacccg
      961 atattgacca gcacgttcac gatcttgatg atggaaaacc gcagcgcttt gttcctgtgc
     1021 cgcaggtaag cgaacgggat ggccgtaaat gcatcgaggg ccacgatcac cgagaacatc
     1081 gtgatgtatt ccgggttgtt gccgtattcg atcagggttg cgacaggctg gctgaatata
     1141 accactaaga acacaaagag cacggaggtt acaaagaggg agacgaggga ggtgctgaag
     1201 acttttttcg gatcgggctc ttttccggcg aaacggaaaa aggcggtctc catgccgtag
     1261 gtcagcaaca ccagcaggaa agccatgtag gcatacagct cggtgatgac accatattcg
     1321 ccggtgatga agattcgggt atagaacggg gtgaggagga agtaattcag cagcctggga
     1381 acgatagtcc ccatcccgta gacaagtgtc tgccctgcca gtttcttaag cgggttcatg
     1441 aattgactga tcgctattta ttgatttctg ccacgatctt tccggatacc gccgttgctg
     1501 cctcttccat cgccttgcgc gcagctttcc ggtaatcgga ccctccccct ttctgcgaaa
     1561 actcctcctg gtaaagcagc tggcccgtcc gggttttcat cagcgataag accacatccg
     1621 cataactgaa ataaaaacca ttcgtgtaag aatattccct tgcttttgca tctatcgtca
     1681 ctttccagtc gctggccgac tcgtcggcgg tgaagctgca ccctttcaga tcccttttca
     1741 acgcattggc taaaatctcc gaaggggagc caaatatatc ttcactgctc tgtacaaaaa
     1801 cgatgacccc tttttccagg tcggccagtt tcctgatggc cgtcgatttc atttgcatcg
     1861 aattggcgta ctgcagcgaa tcgatgttcc ctttaagggc cagcagcacg ccatacgagt
     1921 aatcgatccg ggcaaacagg gggatggctt tctcgagttc cgaccgggct ttcaccttct
     1981 cgccatctgt gtacaaccgg tcgctcaatt caacaacctg gatgatctgt tgctggtaaa
     2041 acccaatatt ggccttgtaa taaccgatga tctcataccg gttggcatag ctgaaagcat
     2101 atacggtagc tgtttcagga ttatagaaca cctcgttggt caggcctgtg agctccatat
     2161 tgctctctgt ttttacagag gcttcataaa actggaccac actggaagtt gattgccggg
     2221 tgatatcctg cactttgctc tccgtataag actgtatctg gaccatcacc ccttcgatca
     2281 gttcgctctg cgccagcttc ttcatcctct cccgggcctg atcgagggtt tcattgacaa
     2341 tctttcctcc ttccatataa cctgtaaggt actggtcggc gggataatga ctttccctga
     2401 aactcttatc gcaccaggca gggatttccg gctgggcaac ggtggcaaga aaaggaaata
     2461 aaacgaaaaa aaggatcggg ttcaggtact tcatcgcttt cagtgataat ggattccatt
     2521 aaaatatctc actgcacatg aaaaggcagt tctaacatca tcttcattta acataatcat
     2581 catggatata gccctgttta cctccgagca gtttgatcag ataatattct ccctgctttt
     2641 caattacggt aaattcagtg cttcccggca ctttggccac gacgtccgag gtttggtcag
     2701 gatttaccct aacgtcgtac ttatccgatt tgttgcctcg cgttgtgact ctttcggcca
     2761 gggcatccga tttctggata aaatcggtct gctcgatatt tatgccatac ccttcgaaca
     2821 ggctaagcat ctctttctct cttattgcag cctgcaccag gggagcatag gttacttcgt
     2881 cgacctcggc ggccagggca taatattccc gggctttttc ataattcccg gctatcctgt
     2941 acaacctggc gatattgctt gaagccgctg aattataagg atcgatgtca taaatggatt
     3001 tataaatgga gaaggcattg ttcaggtcgc cgctttcgag ataatccatc gccagtttgc
     3061 ctttttcttt atactcctta tctttaatct tctcaaactc atattccata ggcaaaaata
     3121 ccggggtgaa ataattggcc agtttggtgg caaggccatc atagcatact tttgccagct
     3181 cttcgacaga aaacaatgtg gatcttttat catcgcattt cttatcggag taggaggtgg
     3241 ttttcacggt agtcccgatg atctgaccgg tattgacatt gataatttta agatgccctt
     3301 ctgccgtcac ggttcgggtg gtacagtaag agtatttttt attcccgttc aggtcggtgt
     3361 aagtaagggt tttctgctca tcctggtgtg aataggccac tgaaccggtg atcatggcat
     3421 ctattcccag aagtttaccg atctgggcgg cctgggagtc gtcgatcaga cccgaattgc
     3481 tgatattttg ttcccgcagc actttatcca gttcactgcg ctccaccagt gaaaaaacat
     3541 tggtccgggc tcctttctga aaattattaa tggatttacc tgcagaaaag tagtttccgc
     3601 ccgattttcc gcctgtgccc ctgaactcct gcatcagttc ggcagcgaga taatcggtca
     3661 tggctgtccc catccttccg tcaaaatcga gaatggaaat cttatggaca tcttccaggt
     3721 atgcttcagg agctaccagc atatacgccg taaagctttg tgaatgccct gaaataatgg
     3781 cgcctgctaa aataaagact gcaataaatg attttttcat ttccttttgg tttaatcaat
     3841 ctttgaacat aaaattagca aataacactt ctgaaataca agtccaatcc ggatttttta
     3901 aaccggtcgg gtaaaatgtc ttgattgacg ggccattcga cgaacagata cgatagtaac
     3961 aaaagcctgt tcagatattt ttatcacgaa atcattgatt tatataattt atccattata
     4021 tttgttttgg aaaaaaaccc aaatgccccc cgaaaggtct gactttgcca gagtccggcc
     4081 ggttattttt tcagtcactt aaagttctta aatcaatcca ccatgcctat caagtatgtg
     4141 ttttccgttg aggatgaact gttaaaagtg acagctcatg gcaaagacga cagcttcgaa
     4201 gaagtgatga attattcccg gacgatcatt gaagcatcca ggcaacatgg ctgcacaaag
     4261 atcctctgtg atgaaaggca ccttcagcac gccattaccg cgaccgacac ttatttgctt
     4321 gctgaaaacg cctctcaaca tgcctcatat ttcagtaaag tagcgataat ctgccataaa
     4381 agatcattaa aggaaggcaa attctatgag acagtcgctt ccaacagggg attaaaaata
     4441 ctggtaacaa ccgatcctac cgcagcattg ggatggctgg acaagtaaca gcatctccct
     4501 catcaaaaaa ttatggaatt acttttacct gaattatgga ataatacttt aaaggcttca
     4561 ataattctaa agatgaaaat gaccaaacta ttttacttac tgccaggact tcccgctctg
     4621 ttcctgatca tagctcctgg tttcggctgc aataaagatg atgacaacat gcctgatgca
     4681 acacctgtcg attattccct ggtggaacac tggctttcgg ttccggctcc ggaaaaggag
     4741 gtggacatat tctacctgta tccgacttca tggcaaaaat taaatcctga cgatcccaat
     4801 atctgcgaca ttgacaatcc gctgatgcag gccggttcgg cgtcggccta tcaccggcag
     4861 gctacggcat ttgcaacaac gggaaacgtc tttgctccct attaccgcca ggcagatgcc
     4921 caatacacgc tcagcctgcc cgaagctcaa agggattcgg tgataggagg tattcctttg
     4981 ctagatgcca cggcagcatt cgactattac atcaatcact tcaatgatgg ccgtccgttc
     5041 atcctggcag gccactcgca gggatccaac atcctgttgt tcctcctttc aggttattta
     5101 aaacaacatc ctgaagttta ttcacggatg attgcggcgt atgttatcgg gtacccggtc
     5161 acccaggcct atctcacagc caatccacac ctgaagtttg ctgagcgcgc cgacgatacg
     5221 ggggtgatca tttcttacaa tacacaatcc ccggccgtca gtcccggtca aaatcccgtt
     5281 gtatctgata ttgtcgggct ggtgatcaac ccgatctcat ggacccggga tgaaaccccg
     5341 gcaactactg ccgaagggtt ggggtcgtat atgcccgacc aggaaggggt gtttgcgcag
     5401 gtaccgcaat atgccgatgc caggattgat accggccagg gggtgctcat ctgttccacg
     5461 gcggtcgata gtaccttata cctgggcttt ggccccgggg tctaccacag ttacgattat
     5521 cctttctatt attttaacct gcgggccaat gccggtctga gagccggcaa gttctta
//
LOCUS       DAOQXS010000142         5549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14926_length_5549_cov_4.082818, whole genome shotgun sequence.
ACCESSION   DAOQXS010000142 DAOQXS010000000
VERSION     DAOQXS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14926_length_5549_cov_4.082818"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PKM34_05250"
     CDS             <1..1424
                     /locus_tag="PKM34_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNQ83029.1"
                     /translation="QANGVRVHLCATIFYGHSVFFNNPAARQNLIDNLISLVQERNAD
                     GVNMDIEAVPSSVSDSMTVFMQDLSIQLKAAIPGAILSIDLPAVDWSNNFQIDELSDY
                     LDWFFVMGYDYYWNGSSEAGPVSPLYSLTPGYDYSLARTVSAYEKAGLAPEKFILGIP
                     YYGRQWKTASDIIPSPVLSNGIALTYSNVRNNSVIYNPEVYRWEPASASSCYVFFLNG
                     YWNQCFIGLDRDLREKYDIANYRNLAGIGIWALGYDNGYPDLWQAVSDKFTDCYIPPV
                     YDTIFDSGGQAWNYYPFEDYEMTIDHGYQDPRFLTFTTCSLEAGYDSLWIYSGSDSGM
                     VFIGSYSGNENPGTITSADGSFTLRFRSDGLTQAPGWQAIFHDGTIGIEPMVKTRDRL
                     LIYPIPAKDEIIIELPKDGRFTGVSINSLSGRTLISAKRLHQPAGSTMRISIGNWPAG
                     TYFVTLAGDRGRGITGMFVKVAE"
     gene            1617..3500
                     /locus_tag="PKM34_05255"
     CDS             1617..3500
                     /locus_tag="PKM34_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNQ83030.1"
                     /translation="MEDNKVKEKLFPEFPPVTVADWEKKINQDLKGADYEKKLVYRPA
                     EGIRIKPYYAADDLLSLEYLNQFPDSFPYIRGNKKSGNDWFIRQDIVVTDLKRANEKA
                     LDILMKGIGSLGFILDDEKEYSKDDLDLLLKNIFAEMVEINFTAGRHAVSLMKNHYEM
                     LVRYNRDFGKIYGSLEFDPFGRLATKGKFYLTEEEDLEICSELIRISGHLPHFTVIAV
                     GGDHFHNAGATIVEELAFSLASGAEYLTRLTEKGLSVNQVAPKIRFRFATGSNFFMEI
                     AKYRAARLLWAHIVKAYGPSREEVSRMTVHATTSKWNKAVYDPFVNMLRTTTESMSSV
                     VAGIDSLTVGPFNAIYQQYDDFAERIARNQQLLLKEESYLDQVADPSAGSYYIESMTD
                     SIAAEAWKLFLEVDSVGGYLQAVKSGFIRERIKATAEKRDAGLANRKEVILGVNQYPN
                     FTEKKEEPLAEQVFSPAKPGFEDLITEPLRLYRGAQPFEALRYKTDMYALKHKRPAAF
                     MFTYGNLAMRIARSQFSRNFFACAGFDTIDNLGFKTIDEGIRVYHESRTEILVICSAD
                     EEYPVIAPAILEALKDKAKVVIAGYPKDSVDELKAKGIEHFIHVRSNVLEELKKFQAL
                     LGL"
     gene            3652..>5549
                     /gene="scpA"
                     /locus_tag="PKM34_05260"
     CDS             3652..>5549
                     /gene="scpA"
                     /locus_tag="PKM34_05260"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNQ83031.1"
                     /translation="MKPNFQKINIISVAAQRISAAEWEQQNRKEEDWLTSEGIPVKHA
                     YTAEDLAGMEHLGYAAGLPPFLRGPYSTMYVQKPWTIRQYAGFSTAEESNAFYRRNLA
                     SGQMGLSVAFDLATHRGYDSDHERVVGDVGKAGVAIDSILDMKVLFDQIPLDRMSVSM
                     TMNGAVLPIMAFYIVAGLEQGARLEQLSGTIQNDILKEFMVRNTYIYPPLPSMRIIAD
                     IFKYTSAHMPKFNSISVSGYHMQEAGATAELELAYTLADGWEYLRTGTQTGLDIDDFA
                     PRISFFWGIGMNHFMEVAKMRAGRMLWAKIVNKFNPKNPKSMMLRTHCQTSGWSLTEQ
                     DPFNNVARTCVEALGAVLGGTQSLHTNALDEAIALPTDFSARIARNTQIFIQEETQVC
                     RTVDPWAGSYYIEYLTDQIARKAWALIEEVEALGGMAKAIETGIPKMRIEEASARKQA
                     RIDSHKDTIVGVNKYRLEKEAPIETLEVDNTAVRESQVKRLHLLKSERNQADVDRILE
                     AITRCCETGEGNLLELSVEAAKARATLGEISYACEKVFGRYKAVIRSISGVYSAESKS
                     DESFRRACELADKFASLDGRRPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLF
                     QTPVETAR"
BASE COUNT         1376 a         1487 c         1474 g         1212 t
ORIGIN      
        1 cacaggccaa tggcgtcagg gttcaccttt gtgccacgat cttctacggg cattcggtat
       61 tctttaacaa ccctgctgcc cgtcagaacc tgatcgataa cctcatctcg ctggtacagg
      121 agcgtaatgc tgacggcgta aacatggaca tcgaggcagt accctcgtcg gtgagcgaca
      181 gtatgacggt ttttatgcag gacctctcca tacaactcaa agcagcaatc cccggcgcca
      241 tcctgagcat cgaccttccg gccgtcgact ggagcaacaa ttttcaaatt gacgaattat
      301 cagattatct cgattggttc tttgtcatgg ggtacgatta ttactggaac gggagttcgg
      361 aggcaggacc cgtctccccg ctctattcgc tcacacccgg ctatgactac agcctggcac
      421 gaaccgtttc ggcttacgaa aaggcaggcc tggcgccgga aaagttcatc ctcggaattc
      481 cctattatgg caggcaatgg aaaaccgctt cggatattat tccttccccg gttctatcaa
      541 atggcatcgc cctgacctat tccaatgtca ggaataactc cgttatatat aacccggagg
      601 tctatcgctg ggaaccggcc agtgcttctt cctgttatgt tttcttcctg aacggatact
      661 ggaaccagtg ttttatcggg cttgaccgcg atttgaggga gaaatacgat atcgccaatt
      721 accgcaacct ggccggaatc ggcatctggg ccttggggta tgataacgga tatccggacc
      781 tgtggcaggc cgtttccgac aaattcaccg attgttatat tccgccggta tacgatacaa
      841 ttttcgacag tggaggccaa gcctggaatt actacccgtt cgaagattat gaaatgacca
      901 tcgatcacgg ataccaggac ccgcggtttc tgactttcac cacttgcagc ctggaagccg
      961 gatacgattc cctgtggatt tattcaggaa gtgattccgg catggttttc atcggaagtt
     1021 attccggaaa cgagaatccg ggaacgatta cctccgccga tggctccttt accctgagat
     1081 tccggtcgga tggactgacc caggcgcccg gctggcaagc catatttcac gacgggacga
     1141 ttggaataga acctatggtg aaaacccgcg atcggctgct aatctatccc attccggcaa
     1201 aagatgagat tatcatagaa cttccgaagg atggacggtt tactggagtt tcgataaata
     1261 gtctttcagg aagaacgctc atcagtgcga agagattaca tcaaccggcc ggtagcacca
     1321 tgagaatcag catcgggaat tggccggcgg gcacttactt tgttacgctg gccggcgaca
     1381 gggggagagg tattaccggg atgtttgtga aggtggcgga atgagtcaaa aaggggacgc
     1441 catcctttgg gacgccatcc ctcgggacgc catcccaaat ccttgttaat ttattcacaa
     1501 taaaccctcc cttgaattat tgttcgtaaa tttgcgcccg gccatagatg gagccatcgt
     1561 ttatcgctcg aataaaaaga taaatgattg ataaacaaga tttagacaga catagaatgg
     1621 aagataataa agtgaaagag aaactcttcc ccgagtttcc gcccgtgacg gtcgccgact
     1681 gggagaaaaa aatcaatcag gacctgaaag gggccgacta tgaaaaaaaa ctggtttacc
     1741 gacctgcgga aggcatccgg atcaaaccct attatgccgc cgatgacctg ctatccctcg
     1801 aataccttaa ccagttcccc gattcgttcc catacatccg cgggaataaa aaatcgggaa
     1861 acgactggtt tattcgccag gatatcgtgg tgaccgacct taaaagggcc aatgaaaaag
     1921 cgctcgatat ccttatgaaa gggatcggct ccctcggatt tatcctggat gatgaaaaag
     1981 aatattccaa agatgatctc gacctgctgc tgaagaatat tttcgccgaa atggtcgaga
     2041 tcaattttac cgccggccgg catgccgtat cgttaatgaa aaaccattac gaaatgctgg
     2101 tccggtacaa ccgtgatttc ggtaagatct acggttctct ggagtttgat ccgttcggcc
     2161 ggctggccac aaagggcaaa ttctacctga cagaggagga agacctggag atttgcagcg
     2221 aactgatccg gatttccgga cacctgcctc attttaccgt tattgcggtc ggtggcgacc
     2281 atttccacaa cgccggcgct accatcgtag aagagctcgc tttcagcctg gcgtcagggg
     2341 cagagtacct cacacgcctt acggaaaaag gattgtcggt gaaccaggtc gcccccaaga
     2401 tcaggttccg ttttgccacc ggatccaatt tctttatgga gatcgccaaa taccgcgctg
     2461 cccgcctgct ttgggcacat atcgtcaaag cctacggacc aagccgtgaa gaggtttccc
     2521 ggatgacggt tcacgctact acctccaaat ggaacaaggc ggtttacgat ccgtttgtga
     2581 atatgctgcg gacaacaacg gaatcgatgt cgtccgtcgt ggcggggatc gattcgctga
     2641 cggtcggtcc ttttaacgcg atctaccagc agtatgacga ttttgccgaa cggatcgccc
     2701 gcaaccagca actcctgctg aaagaagaat cgtatctcga ccaggtggcc gatccgtcag
     2761 ccggttccta ctacatcgaa agcatgaccg acagcatcgc cgccgaggcc tggaagctgt
     2821 tcctggaagt ggattcagtg ggcggatacc tgcaggcggt taaatccgga tttatccggg
     2881 aaaggataaa agccacggcg gaaaaaagag acgccggcct ggccaaccgg aaagaggtca
     2941 ttctcggagt caaccagtac ccgaacttta ccgagaaaaa agaagagccg ttagcggaac
     3001 aggttttctc cccggccaaa cccggctttg aggacctgat cacggaacca ctccgtcttt
     3061 accgcggcgc gcagcctttc gaagcgctac ggtacaaaac agacatgtat gcccttaaac
     3121 acaaacggcc ggccgccttt atgttcacct acggcaacct ggccatgcgg atcgcccgct
     3181 cgcagttcag ccgcaacttc ttcgcctgcg ccggttttga tacgatcgat aacctgggat
     3241 ttaaaaccat cgatgagggt atccgtgttt accacgaaag ccgcaccgag atcctcgtca
     3301 tttgcagcgc cgatgaagaa tacccggtga tcgccccggc gattcttgaa gccttgaaag
     3361 ataaagcaaa agttgtgatt gccggatacc cgaaagattc agttgacgaa ctgaaagcaa
     3421 aagggattga gcattttatc cacgtacggt cgaatgtgct cgaagagttg aagaagtttc
     3481 aggctttgct tgggttataa gatttacttt caaaaaatat tttattatga tttaccacaa
     3541 aggcgcgcaa aggattacac aaaggcgcac gaaggggttc agtatttctg ggatcctttg
     3601 tgttcttcgt gaaatccttt gtgttccttt gtggttataa aataaaaaat catgaaaccg
     3661 aattttcaga agatcaatat aatatcagtc gcggctcaga gaatctcagc tgccgaatgg
     3721 gaacagcaga accgcaaaga agaagactgg ctcacctccg aaggtatccc ggtgaagcat
     3781 gcttacacag ccgaagacct cgccgggatg gagcatctgg gctatgccgc cggcctgccg
     3841 ccgttcctgc gcgggcctta ttcgacgatg tacgtccaga aaccctggac catcaggcag
     3901 tatgccgggt tttccaccgc cgaggaatcc aatgcctttt accgccgcaa cctggcctcg
     3961 ggccagatgg gattgtcggt ggcatttgat ttagccaccc accgcggcta cgattccgac
     4021 cacgagaggg tcgtcggcga tgtgggcaaa gccggcgtgg ctatcgattc gatcctcgat
     4081 atgaaggtcc tgttcgacca gatcccgctc gaccgcatgt cggtgtcgat gaccatgaac
     4141 ggcgcggtgc tgcccatcat ggctttttac attgtggcag ggctggagca gggggcgagg
     4201 ctggagcagc tctccggaac catccagaac gacattctca aagagttcat ggtgcgcaac
     4261 acatacatct atccgccact gccatccatg cgcatcatcg ccgatatctt caaatatacc
     4321 tctgcccaca tgccgaaatt caacagcatc agcgtgagcg ggtaccacat gcaggaggcg
     4381 ggcgctactg ctgagctgga gctggcctac acgctggccg acggctggga atacctgcgg
     4441 acaggaaccc agaccgggct cgatatcgac gacttcgccc cgcgtatctc gttcttctgg
     4501 ggcatcggca tgaaccattt tatggaggtg gccaaaatgc gggccgggcg catgctctgg
     4561 gcgaaaatcg tcaacaaatt caatccgaag aatcccaagt cgatgatgtt gcggacacac
     4621 tgccagacct ccggctggag cctgacggag caggatccgt tcaacaacgt ggcccgcacc
     4681 tgtgtggagg ccctgggcgc cgtgctgggc ggaacgcagt cgctgcacac caacgccctc
     4741 gacgaggcca tcgcattgcc caccgatttt tcggcccgta ttgcccgcaa tacgcagatc
     4801 ttcatccagg aagagacaca ggtctgccgc accgtcgacc cctgggccgg atcgtattat
     4861 atcgaatacc tgaccgacca gatcgcccgg aaagcctggg cgctgatcga agaggtcgaa
     4921 gccctgggcg gaatggccaa agcgatcgag accggcattc ccaagatgcg gatcgaggag
     4981 gcctcggcgc gcaagcaggc aaggattgac tcgcacaaag acactatcgt gggggttaat
     5041 aaataccggc tcgaaaagga ggcgccgatt gagacgctgg aggtcgataa tacggccgtc
     5101 agggaatcac aggtgaagag actccatctc ctcaaatcag agcgcaacca ggccgatgtc
     5161 gaccgcattc tcgaagccat tacccgctgt tgtgagaccg gtgaaggcaa cttgctggag
     5221 ttgtcggttg aggcggccaa agcccgcgcc acgcttggcg agatctccta tgcctgtgag
     5281 aaggtgtttg gaagatataa agcggttatc cgttcgatat caggcgttta ctccgccgaa
     5341 tcgaagagcg acgagagctt ccgccgggcc tgcgagctgg ccgataaatt tgcctcgctc
     5401 gacggacgcc ggccccgtat catgatcgcg aagatgggcc aggacggcca cgaccgcggc
     5461 gcgaaagtgg tggccaccgg ctatgccgac attggcttcg acgtcgatat cggcccgttg
     5521 ttccagaccc cggtggagac cgctcggca
//
LOCUS       DAOQXS010000143         5545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14934_length_5545_cov_4.189253, whole genome shotgun sequence.
ACCESSION   DAOQXS010000143 DAOQXS010000000
VERSION     DAOQXS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14934_length_5545_cov_4.189253"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKM34_05265"
     CDS             <1..646
                     /locus_tag="PKM34_05265"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83032.1"
                     /translation="DNAMLYDEGQFQPLAVQHLAVDLLDDKTRNILYVKGISKKTKGD
                     TLHIFVNHWPSRSEGQETSEWKRCKAADVLKNVTDSIFSPNNQALIVIMGDMNDEPLN
                     ASVSVVLKAYSLTSEIDETGLYNLMSHLFMEGKGTLYYKDWDLFDQIIISGGFWLKQK
                     GLRYSGSSGNIFSPDYLLFTNKEGVSRPNRTAAKDYYGGYSDHLPVYIDLLIKK"
     gene            complement(662..1096)
                     /locus_tag="PKM34_05270"
     CDS             complement(662..1096)
                     /locus_tag="PKM34_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83033.1"
                     /translation="MKKLVFFLVIVVLASCGKKYEEKVIETYPGEKPKRVQYYTTDTD
                     NKYKAKEVFYYENGQKKMEGEYNRDGKKHGKWFYWREDGKMWSEGYFYEGKDDRMRKT
                     WHENGKKHYQGKYDKGVRVGKWKFWDESGKLVKEIDYDKEKE"
     gene            complement(1107..2483)
                     /locus_tag="PKM34_05275"
     CDS             complement(1107..2483)
                     /locus_tag="PKM34_05275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNQ83034.1"
                     /translation="MKDSWYIHNARIVNEGEIYDGGLIIRNGLIERIIHGELPNEELH
                     LVNGLPSLDAGGKYLIPGAIDDQVHFREPGLMHKGEIYTESRAAVAGGVTSFMEMPNT
                     NPQTLTQQLLEEKYRIGSERSLANYSFYMGASNDNLAEILKTDARSVCGLKVFMGAST
                     GNMLVDNHDTLENIFSRVNLLIATHCEDEPVIRANMEQFRELYGEDVPVMAHPLIRSE
                     EACYRSSSLAVQLAEKYNSRLHILHLSSAHEMVLFRNDVPLAEKRITAEVCVHHLWFS
                     DIDYTTLGNRIKWNPAIKSAHDRESLWKALLDDRIDVIATDHAPHTAEEKSGKYFKAP
                     SGGPLVQHSLPAMLDFRKQGRISLERVVEKMCHAPAECFKVEKRGYIREGYWADLALF
                     DDNDPWEVRPDNIYYKCGWSPFEGHTFGSRITHTFVNGNLVYEAEPRILNGNFNEEIR
                     GMRIMFNR"
     gene            complement(2562..3296)
                     /locus_tag="PKM34_05280"
     CDS             complement(2562..3296)
                     /locus_tag="PKM34_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNQ83035.1"
                     /translation="MSDSLVIIPTYNEKENIEKILRFVFGLPREFHVLIVEDNSPDGT
                     ADIVKRLIPEFPDQLFIEERKGKLGLGTAYIHGFKWALARGYDYIFEMDADFSHNPAD
                     LIKLYEASKIHNADVVVGSRYIKGVNVVNWPMGRILMSYYASAYVRFVTRIKVRDTTA
                     GFVCYTRKVLEMINLDRIRFIGYAFQIEMKYTAWKLGFKIYEEPIIFTDRTEGQSKMS
                     SGIFKEAIFGVISLRLRNIRKIHRKI"
     gene            complement(3309..>5545)
                     /locus_tag="PKM34_05285"
     CDS             complement(3309..>5545)
                     /locus_tag="PKM34_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNQ83036.1"
                     /translation="MSHYKSNYNKQKQLASMKKTFVSLTLMTVMSVMMSGLFAQPTGY
                     YNGTENKSGDALKSALNDVISGHTVYSYFYSKEIFKLSDADPNTPGNIIQIYTGKSWP
                     NNDYGSGNDQLNREHIWAKSHGNFGDWQPMDGDVHNLKPVDASVNNDKSNLDYDNGGT
                     PHPEAIGCFFSENNWEPRDQDKGDVARIIFYMDTRYEGENGEIDLTVVDELNTYPEPK
                     HGKLSTLLLWNQMDPPDEFERNRNNVIYSFQRNRNPFIDNPQFAGLIWGNITLSSVSI
                     GNVHQSPEIPVAGQPVTITSTITSSAGAITNATLAYGTSWSNLNQNVTMQGSGNAYSA
                     VIPAQSQGTTVYFKITAQNNTDNASTVIYNFYVPKTFTGTLTSIYDIQGQGPVSPYLD
                     QVVSVSGIVTGNYGANYFLQDGSGAWNGLFIYDQGRNPSIGDSIIVTGTIAEYYEKTE
                     LKSITDYYFISGNHPLPAPAVITCSQAGEAYEGVLIRVNNATCTDDNYMANYYMWTVN
                     DGTGDLLVHNTSVFEYEPSEGQAYNVSGPLDYDFDEWKIQLRLGTDVTTGGDVSAPSV
                     LSVVVLNDSTLKVTFSEPVSAASGENISNYSVNNGVTVIKARLHEFVKSELYLTVSKL
                     TGGSYNLTIQSVADLNGNIMPSPAVLPFSTSFGMDEALSFSCKIYPNPAHGSFRVEFP
                     DNIKMPVSVTIFSVDGREVYSDRWNCNGRSSSTIAVNDLPEGMYVVELTSDRMISHTK
                     LIIK"
BASE COUNT         1349 a         1339 c         1303 g         1554 t
ORIGIN      
        1 cgataatgcc atgctgtatg atgaagggca gtttcagccg ttagcggttc agcatctggc
       61 agtagacctc cttgacgata agacccgtaa tattctatat gttaaaggga taagtaaaaa
      121 gaccaaaggc gacacattgc atatctttgt caaccactgg ccttcacggt cggagggaca
      181 ggagaccagc gagtggaaac gttgcaaggc tgctgatgtg ctaaagaatg tcactgattc
      241 aatattttct cctaataacc aggcactgat cgtgatcatg ggcgacatga atgatgaacc
      301 gttaaatgca agtgtatccg tagtgctcaa ggcgtattca ttgacttctg aaatagatga
      361 aacagggctg tacaacttga tgtcgcacct gtttatggaa ggaaaaggga cgttatatta
      421 caaagattgg gatttatttg accaaatcat tatcagtggc gggttctggc tcaagcagaa
      481 gggactgcgc tactcaggca gttcaggaaa tatattttca cctgattacc tgcttttcac
      541 gaataaagag ggggtttcgc gtccaaaccg taccgcagct aaggattact atggcggcta
      601 cagcgatcat ttaccggttt atattgattt gttgattaaa aaatagcgat tatcaaacaa
      661 actattcttt ttctttatcg tagtcgatct ctttgaccag cttgccgctt tcgtcccaga
      721 acttccattt tcccacacgg acgcccttat cgtatttgcc ctggtagtgc ttcttaccgt
      781 tctcgtgcca ggttttcctc atccggtcat ctttgccttc ataaaaataa ccttcgctcc
      841 acatcttgcc gtcctcacgc cagtagaacc attttccatg tttcttgcca tccctgttgt
      901 actctccctc catctttttc tggccatttt cgtaatagaa tacttccttt gccttgtact
      961 tgttgtcggt atcggtcgta taatactgta ctcttttcgg cttctcaccg ggataggttt
     1021 caattacttt ctcttcgtat ttcttaccgc aggaagccag aacgacaatc accagaaaga
     1081 acactaactt tttcatatat tgagcattat ctgttaaaca ttattctcat tcccctgatc
     1141 tcttcattga aatttccgtt aaggatgcgg ggctctgctt cataaaccag gttgccatta
     1201 acaaacgtgt gggtgatccg cgaaccgaag gtatgtcctt caaatggcga ccatccgcat
     1261 ttgtaataga tattatccgg acggacttcc cacgggtcat tatcatcgaa aagagccagg
     1321 tcggcccagt agccttcgcg gatatacccg cgtttttcta ccttaaagca ctccgccggg
     1381 gcatggcaca ttttctccac aacgcgctcc aggctgatcc tcccctgctt ccggaagtcg
     1441 agcatcgccg gcagcgaatg ttgcaccagc ggcccgcccg aaggcgcttt gaagtatttg
     1501 cccgattttt cttctgccgt atgcggtgca tgatcggtgg caatcacatc gatcctgtcg
     1561 tcgagcaatg ccttccaaag ggactcccgg tcatgtgccg attttatcgc cgggttccat
     1621 ttgatccggt tgcccagggt tgtataatcg atatcggaga accagagatg atgaacgcaa
     1681 acctccgcgg tgatcctttt ttcggccaga ggcacatcat ttcggaacag gaccatctcg
     1741 tgggccgatg aaagatgcag gatatgtaac cgcgaattat acttttcagc gagttgcacg
     1801 gccagggatg aggagcggta gcaggcctct tcgctccgga tgaggggatg agccatcacc
     1861 ggtacatctt cgccgtaaag ctcccggaac tgctccatat tggcccggat gaccggttca
     1921 tcttcgcagt gtgttgcgat gaggaggtta acccggctga aaatattctc cagcgtgtca
     1981 tggttatcga cgagcatatt gcccgtcgag gcgcccataa agaccttcag gccgcaaacc
     2041 gatcgcgcat ccgttttcag gatttcagcc aggttatcgt tggaagcgcc catgtagaaa
     2101 gagtagttag ccagcgacct ctccgatccg atccggtatt tctcttcaag taactgttgg
     2161 gtcaatgtct gtggattggt gttgggcatc tccatgaaag aggtgacgcc gccggctacg
     2221 gcagcgcggc tctcagtgta aatttcaccc ttatgcatca gacctggctc gcggaaatgc
     2281 acctggtcgt cgatcgcccc gggaatcaga tacttacccc ctgcatccag ggagggaagc
     2341 ccgttaacca gatggagttc ttcgttaggc aattccccat ggattatcct ctcaatcaat
     2401 ccgttgcgaa tgatcaaacc accatcgtaa atctcgccct cattgacaat ccgggcattg
     2461 tgtatgtacc agctatcttt cactattagt cgttgatcgt tagtcgttag tcgtttgtaa
     2521 tgaattgatg attttggatc cggatttcaa gttttcaatt tctatatctt cctgtgaatc
     2581 ttcctgatat tccgtaaccg caggctgatc acaccgaaaa tagcttcttt gaaaatccct
     2641 gagctcatct tggattgtcc ttcggtgcgg tcagtaaaga tgatcggttc ttcatagatc
     2701 ttaaatccca gcttccaggc tgtgtatttc atctcgatct ggaatgcata cccgatgaac
     2761 cggatccggt caagattgat catttcaagg actttacgtg tataacagac aaatcctgca
     2821 gtggtatcgc ggacttttat ccgggtaacg aaccggacat aagcggaagc gtaatacgac
     2881 atcaggatac gtcccatagg ccagttcacc acatttactc ctttgatgta gcgtgaacca
     2941 acaactacgt cggcattatg tattttagaa gcttcgtata atttgatcag gtcagcaggg
     3001 ttgtgtgaaa agtcggcgtc catctcgaaa atataatcat aaccccttgc cagggcccat
     3061 ttaaatccgt gaatataagc tgttcccagg cccagcttcc ctttccgctc ctcgataaag
     3121 agttgatcgg gaaattcagg gatcagtctt ttaacaatat cggccgtccc gtcgggagaa
     3181 ttatcttcaa cgatcagaac atggaactcc ctgggaagtc caaaaacgaa ccggaggatc
     3241 ttttcaatat tctccttttc gttataagtt gggataataa caagactatc tgacacttat
     3301 atttggattt atttaataat cagtttggta tgcgaaatca tcctgtcact cgtcagttca
     3361 accacataca ttccttcggg caagtcattc actgcgatcg tggagctgct ccttccatta
     3421 cagttccatc tatccgaata tacttcccgg ccatctacgc taaaaatagt aacggacaca
     3481 ggcattttta tattatccgg aaactcaacg cggaacgatc catgcgccgg attcggatag
     3541 attttgcagc taaacgacag cgcttcatcc ataccgaatg aggtgctgaa aggcaggact
     3601 gctggtgaag gcatgatatt tccgttcaga tcggctaccg actggatggt caggttatag
     3661 cttccgcccg tcagtttgct gacggtgagg tagagttccg atttcacaaa ctcatggagc
     3721 ctggccttga taacggtaac tccgttattt accgagtaat tggaaatgtt ttcgccggaa
     3781 gcggccgaca ccggttcgga gaaggtaacc ttgagtgtgg aatcgttcag aacaacaaca
     3841 gaaagcaccg aaggggcaga gacatcgccg ccggtggtca catcggtccc aagcctgagc
     3901 tggatcttcc attcatcaaa atcatagtcg agtggccctg aaacattata ggcctgacct
     3961 tcggaaggct catattcgaa aacgcttgta ttatgtacca gcaagtcgcc cgtgccgtca
     4021 ttgaccgtcc acatataata gttagccata taattatcgt cggtacatgt agcattatta
     4081 acccggatca ggacgccttc ataggcttcg ccggcctgtg agcaggttat cacggcaggt
     4141 gccggcaagg gatgattccc tgaaataaaa taataatcgg tgatactttt cagttccgtc
     4201 ttctcataat actccgcaat ggttcctgtt acaataatgc tgtcgccgat actgggattt
     4261 cgtccctggt cgtagataaa tagtccgttc catgccccgg agccatcctg gaggaaatag
     4321 ttcgcaccat aatttccggt gacaatcccg gaaacagaga caacctgatc caggtaagga
     4381 ctaaccgggc cctgtccctg gatgtcgtaa atcgatgtga gggtaccggt aaatgttttg
     4441 ggtacataga aattataaat gaccgtgctg gcattgtctg tattattctg cgccgttatt
     4501 ttgaaataaa cggtcgtacc ctgcgattgt gccggaataa cggctgagta ggcatttccc
     4561 gagccttgca tggtcacatt ctggttcagg ttgctccagg aagttccata ggccagggtg
     4621 gcattggtaa tggctccggc tgaagaggtg atcgtcgagg tgatggtcac cggttgcccg
     4681 gcaacaggaa tctcaggtga ttggtggaca ttgccgatcg atacgctgga aagggttata
     4741 ttgccccaga tcaggccggc aaactgcggg ttgtcgatga acggattgcg gttacgctgg
     4801 aaggaataaa taacattgtt gcggttccgt tcgaattcat cgggcgggtc catctggttc
     4861 cacagaagaa gtgtcgaaag cttgccgtgc ttcggttcag ggtatgtatt cagttcatcg
     4921 accactgtca ggtcgatttc gccgttctcc ccttcataac gggtgtccat gtaaaaaatg
     4981 atgcgtgcaa catcaccttt gtcctggtcg cgtggttccc agttattctc gctaaaaaaa
     5041 catccgattg cttccggatg gggtgttccg ccgttgtcgt aatcgaggtt gcttttgtcg
     5101 ttattgaccg atgcatcaac tggctttagg ttatggacat cgccgtccat cggctgccag
     5161 tcaccgaagt ttccatgaga tttcgcccag atatgctcgc ggttcaactg gtcgtttccg
     5221 ctgccgtaat cgttattcgg ccatgactta ccggtgtaga tctggataat gttgccaggt
     5281 gtgtttggat cggcatcgct tagcttgaag atttctttcg agtagaaata agaatatact
     5341 gtatgtccgc tgatcacatc gttgagggcc gatttgagcg catcacccga tttattctcc
     5401 gtcccgttgt aatatccggt tggctgggca aataatccgg acatcatgac agacattaca
     5461 gtcatcaggg tcaggctaac aaaggttttt ttcatactgg caagttgttt ctgtttatta
     5521 taattcgatt tgtaatgaga cattc
//
LOCUS       DAOQXS010000144         5542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_14947_length_5542_cov_4.291781, whole genome shotgun sequence.
ACCESSION   DAOQXS010000144 DAOQXS010000000
VERSION     DAOQXS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_5542_cov_4.291781"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKM34_05290"
     CDS             <1..327
                     /locus_tag="PKM34_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ83037.1"
                     /translation="VMSQDFIRTARAKGLSETRVILRHALKNALNPVITTASGWFASL
                     MAGAVFVEYVFDWKGIGVVVVDALEKYDFPVIMGTVLFISVIMILINILTDIAYSRLD
                     PRVELG"
     gene            complement(477..833)
                     /locus_tag="PKM34_05295"
     CDS             complement(477..833)
                     /locus_tag="PKM34_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ83038.1"
                     /translation="MVKEFPDLERLKHISEATGKILHFCRNKSEDDFIKDEMLNSSVL
                     YQFIIIGETIRYINPELLAKYDYPWHLPKSFRNYIANEYFGINLKQVYKTVTDLLPEF
                     KELIDKMIDTITMLNF"
     gene            complement(826..1416)
                     /locus_tag="PKM34_05300"
     CDS             complement(826..1416)
                     /locus_tag="PKM34_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ83039.1"
                     /translation="MKSFGETIRDLRKKNKLPLRSVADSLGIDVAILSKIERGQRKAS
                     RQFICKISSFYQVDINHLLITWLSDKLAEELIEEDTAMEALQVAEDKIIYGRRKVVDK
                     RQIIDLLVDFFRHDGRISKAWIFGSFARGDNSSHRDIDLMVRYSDKASGTLFDYSDIK
                     FKLENLLKQEIDIVEEGYVQPFALESVNRDKILIYG"
     gene            1715..2485
                     /gene="tpiA"
                     /locus_tag="PKM34_05305"
     CDS             1715..2485
                     /gene="tpiA"
                     /locus_tag="PKM34_05305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ83040.1"
                     /translation="MRTKIVAGNWKMNKTFEEADTLIFEITDLLEEKGKPDDVDVILC
                     PPFIYLELAGDITEDSPVFVGAQNVSRYENGAYTGEISASMLRSMHIDFAIIGHSERR
                     KYFSESDDELLGKVKAALKEDIAPIFCCGELLEERETRQHFDIVRSQLENTVFQLEPD
                     DFARVIIAYEPVWAIGTGINATSDQAQEMHFFIRDLIWKKYGAEIANDKTILYGGSCN
                     SKNAKELFSMPDVDGGLIGGASLKADEFVAIIMAAGEK"
     gene            2549..3397
                     /gene="prmA"
                     /locus_tag="PKM34_05310"
     CDS             2549..3397
                     /gene="prmA"
                     /locus_tag="PKM34_05310"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNQ83041.1"
                     /translation="MNFELKVMGFLEIRFAPVPSDEEKSILIAELLPFGFDSFNEEED
                     AVLAYIDSVVFQDVPFKEIEFLQNHPEIEYRIEPLGDINWNKEWESNYQPVLIGGKCF
                     VRAPFHPENPDAQYNILIEPGMAFGTAHHETTRLMALCLMQLDVKGKKVLDMGCGTGI
                     LAVLANKMGAISVTGIDNDEWAWRNAIVNFGHNGISAENAVCGDASDIKEVAYDMILA
                     NINRNVLLQDMEMYFKGLLPGGRLMLSGFYRKDKETILEKAMATGLQFVGDDHENEWT
                     VILLKK"
     gene            3434..>5542
                     /locus_tag="PKM34_05315"
     CDS             3434..>5542
                     /locus_tag="PKM34_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83042.1"
                     /translation="MLPAFLLVMISSGQSVKSLPEDPARFPVELESVFSSVTSPSAGI
                     QIREMLIPFLEYWNAGLFSEKEQKNIIRQSNIVMGKRLVVFPNLYRYISAVYHLKRFG
                     NEGALTIWMEDFDKQIPESSQGRSEKYLEQYEALFARNRLYESGTYNWYTSDTSIRME
                     YDTAMKIIYRDVLLTCATRKDTSRIYNTSGIFYPGTLEWKGMKGRVTWERVGLDPDSV
                     YADFQDYRINMKKFEYRVDSAGFMNKKYFTRPILGVFKDETLTSPPGPNSTHPQFEAY
                     LNTLEIKDLFRNIDYLGGFTQQGLRITGSGAGDGNASLSLKRGPKEIARIRAKAFRLH
                     QNQVTANPASITIPLDEDSIYHAGLQMKYFVDSAQLLLYQEKDATNRSPFFNSYHDLD
                     MDCGSMEWKIDSNFIDFSTFQVMNDTNLNSFYSGNYYSPAEFYKQQGLDDTNPLYVVK
                     DFCETYGTREIKPDILAHFMKKPVEQVKAMLFNFSSQGFVAYDFVNDKAIVKDKLYHY
                     LDAKAGKIDYDVIGIHSKAVNESNARLNLETKDLDIRGVPMVQLSQAHRVYIFPDSNE
                     IKVKKGLDFVFSGRVQAGPFLFFAHESSFEYDTFKLNMPFIDLIKFRVDDFGKEGGQV
                     TVQSPIADASGKLYIDAPRNKSGKTTIPDFPLFKGEKESYVYYDQDRHYDKEKFAYNV
                     KPFNLAGLDKLQTDDIKFDGY"
BASE COUNT         1530 a         1271 c         1290 g         1451 t
ORIGIN      
        1 gtgatgtcgc aggattttat ccggacggcg cgggccaaag ggctcagcga aacgcgggtc
       61 atcctgcgcc acgctttgaa gaacgctctg aacccggtca tcaccacggc gtcgggctgg
      121 tttgcctcgc tcatggccgg cgcggtgttc gtcgaatatg tcttcgactg gaaagggatc
      181 ggcgtggtcg tcgtcgatgc cctcgaaaaa tacgatttcc ccgtaataat gggtacggtg
      241 ttgttcatct cggtcatcat gatcctcatc aacatcctga ccgatatcgc ctattcccgc
      301 cttgatccga gggtggagct ggggtaggct taagttcaaa gttcaaagtt caaagttcaa
      361 agttcaaagt tcaaagttgt gggtgggcag taggcagtag gcagtgtgta gtgggcagcc
      421 tgtactgagg gcggagccca aaggatcagc agtcattaag aattaagaat taaaaattaa
      481 aaattaagca ttgttattgt atcaatcatc ttatcaatca attctttaaa ctccggtaat
      541 aagtcggtga ctgttttata aacttgtttc aggttaatac cgaaatattc atttgcaata
      601 tagttcctga atgattttgg cagatgccag gggtaatcgt attttgccag caattcagga
      661 tttatatatc ggatggtttc gccaatgata atgaattggt ataagacact actgttcagc
      721 atttcatcct taataaagtc atcttcagat ttatttctac aaaaatgaag gatctttcct
      781 gtggcttctg agatatgctt taacctttcc aggtccggga attccttaac catagatcag
      841 tattttatcg cggttgaccg attcaagagc aaatggctga acataacctt cttcaacaat
      901 gtcaatttct tgtttcagta gattctccag cttgaatttg atatcggaat aatcgaaaag
      961 tgttcctgag gctttatccg aataccttac catcagatca atatcccggt gggaagaatt
     1021 atcgcccctt gcaaacgagc caaaaatcca tgctttagaa attcttccgt cgtgcctgaa
     1081 gaaatcgacc agtagatcaa tgatttgtcg cttgtccacc acctttctcc tgccgtaaat
     1141 gatcttgtct tcagcaacct gtaaggcttc catcgcggta tcttcttcta tcagttcttc
     1201 cgcgagttta tctgagagcc aggtgatcag caaatggtta atatccacct ggtaaaatga
     1261 ggaaatttta caaataaact ggcgtgatgc ttttctttgt cctctctcga ttttactgag
     1321 aatggccacg tcaataccca atgaatccgc aacagacctt agcggaagct tattcttttt
     1381 ccttaaatcc cggatagttt ctccgaaact tttcatggca aatcattttg acaaaattag
     1441 tcaaaaaaca ttgaacactg agaaatgaga aaagagacaa ggggctaatt ttctgccgta
     1501 aaactttaaa cacgaatgag actccatctc gatggcccag atggagtctc gattcttgtg
     1561 agactccatc tcatcggttt gagatggagt ctcgaattgt gcctatgtgg ccaataaaaa
     1621 tcccccagct taagactaaa ttgatttttt tgacttcctg ctgcctactg ccaactgcct
     1681 acttttttaa tatctttgcc aaaaacacca ccgtatgaga accaagattg tagccggcaa
     1741 ctggaagatg aacaaaacct tcgaggaagc cgataccctt atttttgaaa tcaccgacct
     1801 gctggaagaa aaaggcaaac ccgatgatgt ggatgtgatc ctctgccccc cgtttattta
     1861 cctggaactg gccggggata tcaccgaaga ttcacccgtg tttgtcgggg cacagaatgt
     1921 gagccggtac gaaaacggag cctataccgg cgaaatctcc gcctccatgc tccgttcgat
     1981 gcatatcgat ttcgccatta tcggacactc ggaacggcga aagtatttca gcgagtcgga
     2041 cgatgaactg ctggggaagg tcaaagccgc cctgaaagag gatatagcac cgattttctg
     2101 ctgcggcgaa ctgctggaag agcgtgaaac gcgccagcac ttcgatatcg tccgctcgca
     2161 gctcgaaaat accgtcttcc agcttgagcc cgacgacttt gcccgggtga tcatcgcgta
     2221 tgaaccggtc tgggctatcg gcacaggcat caacgccacc tccgaccagg cccaggagat
     2281 gcacttcttt atccgcgacc tgatctggaa aaagtatggc gctgaaatag ctaacgataa
     2341 aaccatcctt tacgggggaa gctgcaattc caaaaacgca aaggaactgt tctccatgcc
     2401 cgacgtcgac ggaggcctga tcggtggcgc ttcgctcaaa gcggatgagt ttgtcgcgat
     2461 cattatggca gccggagaaa aatagttcaa agttcaaaga tcaaagtgca aagtttttta
     2521 tgtttgcttt gactttgaac tttgaacttt gaactttgaa ctaaaagtga tgggttttct
     2581 cgaaatcaga tttgcaccgg ttccgtcgga cgaagaaaaa tcgatcctga tcgccgaact
     2641 gttgcctttt ggtttcgata gttttaatga agaagaagat gccgttctgg cttatatcga
     2701 ctctgttgta tttcaggatg ttcctttcaa agagatcgaa tttctgcaaa atcatccgga
     2761 aatagaatac cggatcgaac ccctcggaga tatcaactgg aacaaagaat gggagtcgaa
     2821 ttaccagccg gtgctgatcg gcggtaaatg ctttgtcagg gcgccttttc atcctgaaaa
     2881 tccggatgcg caatacaata tcctgatcga accgggaatg gccttcggta cagcccacca
     2941 cgagacgacc cgcctgatgg cgctttgcct gatgcaactc gatgtaaagg ggaaaaaagt
     3001 actggacatg ggttgcggca caggcatcct ggccgtcctg gccaataaaa tgggcgccat
     3061 aagcgttaca ggtattgata acgacgaatg ggcctggcgt aatgcgatcg tcaatttcgg
     3121 acataacgga atttcagcgg agaatgctgt atgcggcgat gcatcagata tcaaagaagt
     3181 ggcttatgat atgatcctgg ctaatatcaa ccgcaatgta ctgctccagg atatggagat
     3241 gtatttcaaa gggctgctgc ccggcggcag gctgatgctc agcggttttt acaggaaaga
     3301 taaggaaact atcctggaaa aagccatggc gacaggattg caattcgttg gcgacgatca
     3361 tgaaaatgag tggactgtca ttcttctgaa aaaataatac cggctgctta tgtcattcag
     3421 gaaaatttct ttgatgctcc cggctttcct tctggtgatg atctccagcg gccagtcagt
     3481 aaaatcattg ccggaagatc cggcccggtt tcccgtggaa ctggaatcag tcttttcatc
     3541 agtcaccagc ccgtcggcag gaatacagat cagggaaatg ctcatacctt tcctggaata
     3601 ctggaacgcc gggctcttca gtgaaaaaga gcaaaaaaac attatccggc aatccaatat
     3661 tgtcatgggc aaacgactgg tcgttttccc aaacctgtac cgttatattt cagcggttta
     3721 tcacctgaaa agatttggta atgaaggcgc actgaccatc tggatggaag acttcgacaa
     3781 acaaatcccc gaatcttccc agggccgatc ggaaaaatac ctggagcaat atgaagcgct
     3841 cttcgcccgg aaccggctct atgaatcggg tacttataac tggtacacat ccgatacctc
     3901 catccggatg gagtatgata cggccatgaa gatcatttac cgcgatgtgt tgctgacctg
     3961 cgccacccga aaagacacct cgcggattta caatacttcg ggtatctttt atcccggcac
     4021 cctggaatgg aagggcatga aaggcagggt aacctgggaa agagtaggat tggatcccga
     4081 ttcggtctat gccgatttcc aggattaccg gataaatatg aaaaagttcg aataccgggt
     4141 cgattcggcc gggttcatga ataaaaagta ttttaccaga ccgattctcg gcgttttcaa
     4201 agatgaaacc ctgacgagcc cgccgggtcc caattcgact catcctcaat tcgaagccta
     4261 cctgaatacc ctggagatca aagatttgtt tcgaaatatc gattacctgg gcggatttac
     4321 ccagcaaggg ctcaggatca ccggttccgg cgccggagat ggaaatgcca gcctgtcgct
     4381 gaaaagagga ccaaaggaaa tagcccggat acgagccaaa gccttccggc ttcaccaaaa
     4441 ccaggtcacg gctaatcctg catccatcac tattcccctg gatgaggatt cgatctacca
     4501 cgccggattg cagatgaaat attttgtcga tagcgctcaa ctgctccttt accaggaaaa
     4561 agatgccacg aaccgcagcc cgtttttcaa tagttaccat gacctggata tggattgcgg
     4621 atcgatggaa tggaagattg acagcaattt tatcgatttt tccaccttcc aggtgatgaa
     4681 tgataccaat ctgaattctt tctattccgg caattattat tctccggcag aattttacaa
     4741 acaacagggg ctcgatgata ccaacccgct ttacgtggtt aaagattttt gtgagactta
     4801 tggaacccgt gagattaagc ccgatatcct ggctcatttt atgaaaaaac ccgtggagca
     4861 ggtcaaggcc atgttgttca atttcagttc acagggattt gtggcttatg attttgtgaa
     4921 cgataaagct attgtcaaag ataagttgta tcactacctg gatgccaagg ccggaaaaat
     4981 tgattatgat gtcatcggta tccattccaa ggcggttaac gagagcaatg ccaggctgaa
     5041 cctggaaacc aaggacctgg atatccgcgg ggttccgatg gtccagctta gccaggcaca
     5101 cagggtttat atcttccccg acagcaacga aataaaggtg aagaaaggcc tcgattttgt
     5161 gttctcaggc agggtgcagg ccggaccttt cctttttttc gcgcatgaat cctcctttga
     5221 atacgatact tttaagctga atatgccttt tatcgacctg ataaaattca gggtggacga
     5281 ttttggtaag gaaggcggcc aggttacagt ccagagcccg attgccgatg ccagtggaaa
     5341 actatacatc gacgcaccca ggaataaatc gggaaaaact accatacccg attttcctct
     5401 ttttaaaggt gaaaaggaat cttatgttta ctacgaccag gatcgtcatt atgataagga
     5461 aaagttcgca tataatgtca aaccgttcaa tctcgccggg ctcgataagc ttcaaaccga
     5521 tgatataaaa tttgacggtt ac
//
LOCUS       DAOQXS010000145         5524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15010_length_5524_cov_3.276102, whole genome shotgun sequence.
ACCESSION   DAOQXS010000145 DAOQXS010000000
VERSION     DAOQXS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15010_length_5524_cov_3.276102"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1667)
                     /locus_tag="PKM34_05320"
     CDS             complement(75..1667)
                     /locus_tag="PKM34_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HNQ83043.1"
                     /translation="MYLCKLEIENFRGIRFARMNLNETTVVTGENDSGKTTLLDAVCQ
                     VLSPSGESHGIIFTAGDFHMRSEQPEIRPSGSIGIRLTFRERRPDEWSSLRLNDYGFG
                     LEDDQETLQELTLEVKSGVLTERGEARASWRIRTAGQTEMAFSEDMAILDWFRRLNPV
                     FRIRSDMFSKANLDFSREPGIFEDFRTSIKYVENHSNIFRSDGIPMEDIIYDILGKKS
                     DSNSGETRRIVLHHGSAAEKIGMLVFIEAFLESGGLKADASAEPIIIIEDPEAHLHPM
                     TLQSVKILIERMNWQKIITTHSGSLLSDFALEDIRRITRREGTIREYRVKPGSLSSEE
                     LRRLSYHVRKMIPNATFARCWLLVEGESEMWLMPHLARLCGYDFAMEGVVCVEFAQCG
                     IAPLVKAASQLGIEWFLMADGDAAGRAYIETARHFARQLNREPEEHCLRFREKDIENH
                     LFFNGYADIYSHYSGIPVHDSENMQPRRIIGRAIHRNSKPFMAIAIIEAMARTGSQGV
                     PSELRKVVEQCVRLAGGSENSR"
     gene            1760..2290
                     /locus_tag="PKM34_05325"
     CDS             1760..2290
                     /locus_tag="PKM34_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83044.1"
                     /translation="MKRLIIKLLALSFMLTISLGSFSQTDEDMKAMVAKMNDEMMELL
                     KAGRYEAMAKYYDINLISMPNYRVMEKGWQLILNNNLGRKKGGYQVADGKKTTTDLII
                     GEDMMVDLGQYTLTINFPGLEKPKVDNGKYMNVWRKGKDGSWRIVVETWNADKSPNAP
                     AQPKPTMQPAGQPVKK"
     gene            2358..2942
                     /locus_tag="PKM34_05330"
     CDS             2358..2942
                     /locus_tag="PKM34_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNQ83045.1"
                     /translation="MNNPARIREAIKDSIGVKEILAADPEMIRTIEMIAETCSRALCD
                     GQKIMFCGNGGSAADAQHLAAELSGRFYFDRKPMAAEALTVNTSYLTAVSNDYSFEDV
                     FSRLVEGSGRPGDILVGLSTSGNSENVVRAFAKAHESGIITVALTGEDGGKLAPLADY
                     LICVPSADTPRIQESHILIGHIICELIENHLFRV"
     gene            2939..3649
                     /locus_tag="PKM34_05335"
     CDS             2939..3649
                     /locus_tag="PKM34_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ83046.1"
                     /translation="MTGEAIILAGGYGTRLKGVIPDGPKAMAPINGRPFLEYVMDYLD
                     RWGLNHIVLSVGYQQEKIINHFGTGYKSLAIDYALEDEPLGTGGAILNALPLIKGFSS
                     FIFNGDTYFDVNLQRLDDFRRIKEADLCLAIRFEIDPGRFGVLEFDNNNRITGFNEKS
                     ADIEEGYINGGVYILRKDYLLRFGLPRKFSFENDFLQKYHSTEEFYGMRCFSYFRDIG
                     IPEDYNKAVDEFKRLIFY"
     gene            3621..4202
                     /locus_tag="PKM34_05340"
     CDS             3621..4202
                     /locus_tag="PKM34_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNQ83047.1"
                     /translation="MNSKGLFFTKDWTLFLDRDGVISERLPGDYVRKWEDFRFIGGVF
                     ESLRMFSGIFGRIIIISNQQGVGKGLMTAEELHEIDREMKLEIGNSGGRIDAAFYCTS
                     LASENNHDRKPGVGMGLKAKALYPEIDFSKSVMVGDSDSDMEFGKKLGMVTVYIGSDK
                     NCLENADYVFDSMFSFAQSINESHHREHRFTQS"
     gene            4208..>5524
                     /locus_tag="PKM34_05345"
     CDS             4208..>5524
                     /locus_tag="PKM34_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNQ83048.1"
                     /translation="MTPSLILIIFLLYTALLFFITWLTARKATNRSFYIGNKESPWIV
                     VAYGMIGASLSGVTFMSVPGWVGDTQFSYMMVVLGYLIGYFIIATVLMPLYYRLNLTS
                     IYSYLEKRFGFWSYKTGAFFFLMSRTIGASFRMFLVVNVLQVFVFDAWGIPFIATTLI
                     FIILILLYTFKGGIKTIIWTDTVQTTFMLTALVITIYLVTREMNMDFRQMFNTVWESD
                     YSRMIFGDWHDKRFYLKQILSGAFIAIVMTGLDQEMMQKNLSCRTLRDAQKNMFSFSM
                     VLVLVNFLFLFLGAALYLFAHDKGIDLPHRSDDLFPVIAINYLGPVAGIVFIIGLISA
                     AYPSADGALTSLTTSFSIDFLGLNKKDHLSEKQKVKARYLVHSGFALLLLVVIVIFRA
                     INDRAVIDKLFTIAGYTYGPLLGLYAFGLFTKWKETHLQHIIPKNVK"
BASE COUNT         1484 a         1276 c         1276 g         1488 t
ORIGIN      
        1 tttctttgtt aaattaatat tttaaattac agggtgcaaa gatacatctt ttttccccga
       61 ctaacgacca acgactaacg actattttcc gacccgcctg ccaacctgac gcactgttct
      121 accacctttc tgagttctga tggcaccccc tgtgatccgg tccgggccat cgcctcgata
      181 atcgcaatag ccataaacgg tttggaatta cgatggatcg cccggccgat aatccttctg
      241 ggctgcatat tttcgctgtc atgcaccggg atgcccgaat aatgggaata gatatcggca
      301 taaccattaa agaacaaatg attctcaata tctttctccc tgaacctcag acaatgctct
      361 tcaggctcac ggtttaactg ccgggcaaaa tgcctggcgg tttcaatgta agcccttccg
      421 gcggcatctc catcggccat caggaaccat tcaatgccta gctgtgaggc ggcttttacc
      481 agtggggcta tgccgcactg ggcaaattcc acacaaacca ccccctccat ggcaaaatca
      541 tagccgcata acctcgccag gtgaggcatc agccacatct ccgattcccc ttcaaccagc
      601 aaccagcagc gggcaaaagt ggcattcggg atcattttcc tgacatgata actcagccgg
      661 cgcaattctt cactgctcag gctcccgggt ttgacccggt attcccggat agttccctct
      721 cgccgggtga tgcgccggat atcctccagg gcaaaatccg ataacaggct gccggaatgc
      781 gtggtaatga tcttttgcca gttcatccgt tcaatcaata tcttgacaga ttgtaacgtc
      841 atcggatgta aatgggcctc ggggtcttcg atgataatga taggttcggc agatgcatcc
      901 gctttcaatc cgcctgattc aaggaatgct tcgataaata ccagcattcc gatcttttcc
      961 gcagcagaac catgatgcag aacgatccgg cgtgtttcac cactatttga atccgatttt
     1021 ttacccagga tatcataaat aatatcctcc atgggaatac catcgctccg gaatatattg
     1081 gaatggttct caacgtattt gatggaagtc cgaaaatctt caaaaatacc gggctcacgg
     1141 gaaaaatcca gattggcttt cgaaaacata tcgctcctga tcctgaatac cggatttagc
     1201 ctgcggaacc aatccaggat tgccatatcc tccgaaaagg ccatttcagt ttgcccggct
     1261 gttctgatgc gccatgaggc cctggcttca ccccgctccg tcagaacacc cgatttcacc
     1321 tcaagagtca gctcctgcag ggtctcctga tcatcctcca gcccgaaccc ataatcattc
     1381 aaacgtaaag atgaccactc atccggcctt ctttccctga aagtaagccg gataccgatc
     1441 gaaccggaag gcctgatctc cggttgttca gatcgcatgt gaaaatcacc ggctgtgaaa
     1501 atgatgccat ggctttcacc ggaaggcgaa agtacctggc aaacagcgtc cagcaaggtc
     1561 gttttacccg aatcgttttc cccggtgacc accgtcgtct catttaaatt catccgtgca
     1621 aaacgaattc cacgaaaatt ctcaatttca agtttgcata agtacatgca tcaaaattaa
     1681 aactattttt tcgcgtttta aattatccta tctttggaac cgttttaaat aattatgaag
     1741 actaaaataa tcctgatgta tgaaaagact cataattaag cttttagctt tatcattcat
     1801 gctaacgatc tcattaggtt cattcagtca gaccgatgaa gatatgaaag ccatggtggc
     1861 aaagatgaat gacgagatga tggaattact gaaagcggga aggtatgaag cgatggccaa
     1921 atattatgac ataaacctga tctcaatgcc gaattacagg gttatggaaa aaggttggca
     1981 attgatcctg aacaataatc ttggacgtaa gaaaggagga taccaggtag ctgacggtaa
     2041 aaaaaccaca actgacctga taatcggtga agatatgatg gtcgaccttg gccagtatac
     2101 gctgactatt aattttcccg ggttggagaa gccaaaggtg gataatggaa aatacatgaa
     2161 tgtttggagg aagggtaaag atggttcctg gcggattgtt gtggaaacct ggaatgccga
     2221 taagagcccg aatgctcccg ctcaaccgaa accgaccatg cagccggcag gtcaaccagt
     2281 gaaaaaatag ccgaccctct ttcaaccgct ttgcattata aaacaataaa ccagtctttc
     2341 agcctttgaa accagaaatg aataatccag cccggataag agaggcaatt aaagattcga
     2401 tcggggttaa agagatactc gctgctgacc ctgaaatgat ccggaccatt gaaatgattg
     2461 cagaaacgtg ttcacgagcc ctgtgtgacg gtcagaagat catgttttgc ggcaacggtg
     2521 gaagtgctgc agatgctcag cacctcgcgg ctgagctttc aggacggttc tatttcgaca
     2581 ggaaaccaat ggcggcggaa gcactaacgg tcaatacatc ttatttgacg gctgtttcaa
     2641 acgattatag ttttgaagat gttttttcgc gcctggttga aggttcagga cggcccggag
     2701 acatcctggt aggcctttcc acctccggga attcggaaaa cgtggttagg gcctttgcca
     2761 aagcccatga gagcggaata attacggtgg cactgaccgg tgaagatggc ggaaaactgg
     2821 ctccattagc cgattacctg atatgcgtcc cttctgctga tacgcccagg atacaggaat
     2881 cccatatcct gatcgggcat atcatctgcg aattgatcga aaaccacctt ttccgggtat
     2941 gacaggagaa gccattattc ttgccggtgg ttacggtaca aggttgaaag gggtcatacc
     3001 cgacggcccg aaagccatgg cgcccatcaa cggcagacct tttctggaat atgtcatgga
     3061 ctatctcgac cgttggggac tcaaccacat cgtcttgtcc gtcggatatc agcaggaaaa
     3121 gatcataaat catttcggaa caggatataa atcactggcg attgactatg ccctggaaga
     3181 tgaaccgctc ggaaccggcg gcgccatcct gaatgccctg ccacttataa aaggtttttc
     3241 aagtttcatt tttaacggcg acacctattt tgatgtcaac ctgcaacgcc tcgatgattt
     3301 caggcgtatc aaagaagccg acctgtgcct tgctatccgt tttgaaattg atccgggacg
     3361 gttcggagtg ctggaatttg acaataacaa ccggattacc ggtttcaatg agaaatccgc
     3421 tgatattgaa gaaggttata ttaacggcgg ggtctacatt ttgagaaaag actatctgct
     3481 caggtttgga ttgccccgca aattcagttt cgaaaacgac tttctccaga aataccattc
     3541 aacagaggaa ttctacggta tgagatgttt ctcttatttc agggatattg gcataccgga
     3601 ggattataac aaagcagtcg atgaattcaa aaggcttatt ttttactaag gattggacct
     3661 tgttccttga ccgcgatggt gttataagtg aacgccttcc cggtgattat gtcaggaagt
     3721 gggaagattt ccgttttatc ggcggcgttt ttgaatcttt gagaatgttt tcagggatat
     3781 tcggcaggat catcattata agtaatcagc agggagtggg gaaaggattg atgacggcgg
     3841 aggaattgca tgaaatcgac cgggaaatga aattggaaat cggaaattcg ggggggagga
     3901 tcgatgcggc attctattgt accagtttgg cttctgaaaa caatcatgac cggaaaccag
     3961 gggtggggat ggggctcaag gcaaaggccc tttacccgga gatcgatttc tcgaaatcag
     4021 tgatggtagg ggatagtgat agcgatatgg aatttgggaa aaaactcgga atggtaacgg
     4081 tatatattgg atccgacaaa aactgtcttg aaaatgcaga ctatgttttt gactccatgt
     4141 tttcttttgc gcaatcgata aatgaatctc accacagaga acacagattt acacagagtt
     4201 aacacagatg acgccatcac tgatattaat catttttctg ctctataccg cgctgctgtt
     4261 ttttatcacg tggctcactg ccagaaaggc tacaaacagg tcattctata ttggtaataa
     4321 agaatcgccc tggatagtgg tggcctatgg catgattggc gcatccctgt cgggggtgac
     4381 ttttatgtcg gtgccgggct gggtgggaga tacccagttt tcctatatga tggtcgtatt
     4441 gggatacctg atcggttatt tcatcattgc gaccgttttg atgccactgt attacaggct
     4501 gaacctcact tccatttatt cgtacctcga aaaaaggttt ggcttctggt cgtataagac
     4561 aggggcattt tttttcctga tgtcgcgcac tatcggggct tccttcagga tgttcctggt
     4621 agtcaatgta ctccaagtat ttgtgtttga tgcctggggc atacctttta tagccacaac
     4681 actcattttt atcattctta tcctgctcta tacttttaaa ggcggtataa aaacgattat
     4741 ctggaccgat actgtccaaa cgacattcat gcttacagcc ctggtcatta ccatatatct
     4801 tgttacacgt gagatgaata tggatttcag gcaaatgttc aatactgtct gggagagtga
     4861 ttatagccgg atgatattcg gtgactggca cgataaacgc ttttacctga aacagatcct
     4921 tagcggagct tttattgcta ttgtcatgac ggggcttgac caggaaatga tgcagaagaa
     4981 tctgagttgc aggactctga gggatgccca gaagaacatg ttctccttta gcatggtcct
     5041 ggtgctggtc aactttcttt tcctttttct cggagccgcc ctttatctct ttgctcacga
     5101 caaaggcatt gacctgccac atcggtccga cgatcttttc cccgttatcg ctatcaatta
     5161 tctcggacca gttgccggta tcgtattcat catcgggctc atttcggccg catatcccag
     5221 tgccgacggt gcactcacgt cgctcaccac atcgttcagc attgatttcc tgggcttgaa
     5281 taaaaaggat cacttatcag aaaaacaaaa agtaaaagcc aggtacctgg ttcattccgg
     5341 gttcgcgttg ctgctgctgg tggtcattgt gattttcagg gctatcaacg accgggcagt
     5401 catcgacaaa ctcttcacta ttgccggtta tacgtacggg cccttgctgg gtctttatgc
     5461 tttcgggctg tttacaaaat ggaaagaaac acatttacaa catattattc ccaaaaatgt
     5521 taaa
//
LOCUS       DAOQXS010000146         5523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15015_length_5523_cov_3.979151, whole genome shotgun sequence.
ACCESSION   DAOQXS010000146 DAOQXS010000000
VERSION     DAOQXS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15015_length_5523_cov_3.979151"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKM34_05350"
     CDS             complement(<1..295)
                     /locus_tag="PKM34_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83049.1"
                     /translation="MKTLVDSILTKGILTKDDLVYLLSTKEEKEKHSLFRKANQVKKE
                     HCGNVVYLRGLIELSNICRKDCNYCGIRKSNEKVVRYTLPEDEVRANMKMTQEL"
     gene            complement(418..1848)
                     /gene="hydG"
                     /locus_tag="PKM34_05355"
     CDS             complement(418..1848)
                     /gene="hydG"
                     /locus_tag="PKM34_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNQ83050.1"
                     /translation="MKFQPQQYNLPDERMKPFIDPDEISSLLARSFPTREQVREVLAK
                     ALAKNRLSMEETAILVNATDPQLVEEIKAAARKLKKDVYGERIVLFAPLYVGNYCVNN
                     CKYCGFAASNRKALRKTLTNEELVRNVEALEENGQKRLILVYGEHPSYTPEYIAETVR
                     TVYNVKKGRGEIRRVNINAAPLDIEGFRTVKEAGIGTYQIFQETYHPETYKKYHTGGF
                     KRDYENRLTSLDRAQEAGLDDVGIGVLFGLYEWRYEVLALVRHANHLEAVYNVGPHTI
                     SFPRLQFASEYEISGDWLVSDDDFTKLVAILRLAVPYTGLILTAREPEHIRREVLQFG
                     VSQIDAGTKLEIGAYAEEELPTAQNPDKEQFLINDNRSLREVIDELLEEGFIPSFCTA
                     CYRKGRTGEHFMEFSVPGFIKRYCTPNALLTLAEYLEDYAPAPTAEKGWELILKNLEE
                     EVDPKFTAEVKKRLERIKAGERDLYF"
     gene            complement(1932..3059)
                     /locus_tag="PKM34_05360"
     CDS             complement(1932..3059)
                     /locus_tag="PKM34_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNQ83051.1"
                     /translation="MATLKVLVVDDEPGIRSGIIRIMRNFRVGYPFMDEDFDFEVIEA
                     ETGERAIEIIEAQPMDIILLDNKLPGMDGIEVLDYIKKKEYDAQVMMITSYASLDLAI
                     KATNTGAYNFMPKPFTPDELKSSIEGITKHLYLKRMTRNMNREGKQVRFQFLSVLSHE
                     LKSPINAVEGYLKIMKERQVGDNLADYDTMIDRSLERIKGMRNLIMDLMDLTRLESGK
                     KVREMRKVDLNQVARIAMDTMEPMAIQKNVKMYLDAPENLTMVGDPDEIEIIVNNLVS
                     NAVKYNREGGSVNVMMRQSSSSLNITVEDTGIGMSEDDIKLLFQEFVRIKNEKTKLIT
                     GSGLGLSIVKKLVEETYKGTISVKSQPDVGSTFVVVLPLIL"
     gene            complement(3135..3530)
                     /locus_tag="PKM34_05365"
     CDS             complement(3135..3530)
                     /locus_tag="PKM34_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ83052.1"
                     /translation="MKESKKTILIVDDDIDYLSQMELQVKRLGYDVVTADTQKEGEEL
                     IGKGKFDLAIIDLMMENKDSGFILCYKMKQKNPAMPVIIASAVTAETGLSFGVETAQD
                     RSWIKADTFIEKGIRPDQLEREINKLLKI"
     gene            complement(3614..>5523)
                     /locus_tag="PKM34_05370"
     CDS             complement(3614..>5523)
                     /locus_tag="PKM34_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83053.1"
                     /translation="VRECPVKAIRIFNGQAEVIAERCIACGNCVRVCSQDAKVFIDSR
                     EQVKIFLKGDRPVYAVIAPSFPAEFREIKDYRVLVGMIKKLGFEKVVEVSFGADMVAM
                     EYNRLLGGNGGKHYISSDCPAIVNYVENYHPDLVPSLAPIVSPMVALCRVIRKKYGEE
                     PLIVFIGPCIAKKAESDEIDEAITFRELRKLFQEFNVTPEQTQPAEFDEPVSGKGAIF
                     PVSRGLLQTANKNDDLVEGNIMVADGRVNFREAIKEFENGLMNSQHLELLCCEGCIMG
                     PGMTYGCKKFEGMALVSEYVREKMQNLDEEKWENQVEEFSGIDFSQQFSRKERTPLQA
                     PKEKVDEVLFKMGKVSEKDQLNCGACGYDTCVDHAIAIVKGLAENEMCLPYAIEKLHT
                     SLKDLNKSNEQLATTQQALRQSEKLASMGQLSAGIAHELNNPLGVITMYTNILKDETP
                     PDSPVYKDLQLIVEQAERCRGIVGGLLNFARKNQVFLVETNTVDFVKRSVDSIIRPDN
                     IDIKIENKLSDPIARIDKDQMMQVLTNLEKNAVEAMPKGGTLKISLTGTDHEVELAIS
                     DTGIGIPEENMEKIFTPFFTTKEVGKGTGLGLPLIYGIVKMHRGQVQVTSNADPWKGP
                     TGTTFTIKIPR"
BASE COUNT         1184 a         1466 c         1259 g         1614 t
ORIGIN      
        1 caagttcttg ggtcatcttc atgttcgccc ggacctcatc ctcaggcagg gtgtacctga
       61 caaccttttc attgcttttc ctgatcccgc agtaattgca gtcttttctg cagatgttgg
      121 aaagctcgat caatcccctg aggtacacca cattgccgca atgttctttt ttaacctggt
      181 ttgccttgcg gaaaagggag tgcttctctt tttcttcctt cgtcgataac aggtagacca
      241 ggtcatcttt tgttaaaatg cctttggtta aaatactgtc taccaatgtt ttcatttctt
      301 ctttttgttt atcccctacg gggaaaattg atatctggga ttaatccggt ctaccataat
      361 atatccgcta cgcggaatta tatcgaccat cgaaaatcga caatcgacaa tcgacaatca
      421 aaagtacaaa tcccgctctc ccgccttgat ccgttcaagc ctctttttca cttccgctgt
      481 aaatttcgga tccacttctt cctcaaggtt tttcaatatc agttcccagc ctttctcagc
      541 cgtcggcgcc ggggcataat cttccaggta ttcggccagg gtgagcaagg cattgggtgt
      601 acaatatctt tttataaagc cggggaccga aaactccata aagtgctcgc ccgtcctgcc
      661 tttcctgtag caggctgtgc agaaagacgg gataaagcct tcttcaagga gttcgtcgat
      721 cacttcgcgg agagaacggt tatcgttgat cagaaactgc tctttatcag gattttgtgc
      781 tgtagggagt tcttcttccg cataagcgcc gatctcgagc ttagtaccgg catcgatctg
      841 cgaaacgccg aactgcagga cttcgcggcg gatatgttcc ggttcgcgtg cggtcaggat
      901 cagcccggta taaggaacgg ccaggcggag aatggctacc agcttcgtga aatcatcatc
      961 gctcacgagc cagtcgcccg atatttcata ttccgatgca aactgcaatc gcggaaagga
     1021 gatggtgtgc ggcccgacgt tgtaaacggc ttcgaggtgg ttggcgtggc gaaccaacgc
     1081 caacacctcg tatcgccatt catacaaacc aaacagaaca ccaatgccga catcatccag
     1141 accggcttcc tgtgcgcggt cgagcgatgt caggcgattt tcgtaatctc ttttaaatcc
     1201 tccggtatgg tattttttat aggtttccgg atgatatgtt tcctggaaga tctggtatgt
     1261 accgatcccg gcctctttga cggtcctgaa accttcaata tccagcgggg cagcattgat
     1321 attgaccctc ctgatctctc ccctgccctt ttttacattg taaaccgttc taactgtttc
     1381 ggcaatatat tcgggggtat aggaaggatg ctcgccgtag accaggataa gccgtttctg
     1441 gccgttttcc tcgagggctt cgacgtttct gaccagttct tcgttggtta aagtcttcct
     1501 gagcgctttc cggttggaag ccgcaaagcc gcagtatttg cagttattta cacaataatt
     1561 cccgacgtaa agcggagcga aaaggacaat tcgttcacca taaacgtctt ttttaagttt
     1621 cctggcagca gctttaatct cttcaacaag ttgaggatca gtggcattga ccaggattgc
     1681 ggtttcctcc atggagaggc ggttcttggc cagggctttt gcgagaactt ccctgacctg
     1741 ctcccgggtt ggaaaagagc gggcgagcag agaactgatt tcatcgggat cgatgaaagg
     1801 cttcatccgc tcgtccggga gattatattg ttgaggttgg aatttcattt tattctattt
     1861 atagcacgcg gatgacgcgg atgcgacgga tgaccacgga ttattattat tcactattca
     1921 ttattcttta ttcacaggat tagtggcagc acaaccacaa aagtgctgcc cacatccggc
     1981 tggcttttga cgctaattgt gcctttgtat gtttcttcca ccagtttctt cacgatcgat
     2041 aggccaagtc cgctgccggt gatcagtttc gttttttcat ttttaattct tacaaactcc
     2101 tggaaaagaa gcttgatgtc atcttcgctc attccaattc ctgtatcttc tacagtaata
     2161 tttaaagatg atgaggattg acgcatcatg acattgaccg aacctccttc ccgattatac
     2221 ttaacggcat tcgacaccag gttgttcacg atgatctcaa tttcatcggg atcgcccacc
     2281 atcgttaaat tctccggggc gtcgaggtac atctttacat ttttttgaat cgccatcggc
     2341 tccatggtgt ccatcgcgat cctggccacc tggttgaggt cgacttttct catctcacgc
     2401 actttctttc ccgattccag cctggtcagg tccatcaggt ccatgatcag gtttcgcatc
     2461 cctttgatcc gctccagcga acggtcgatc atggtgtcgt aatcggcaag gttatcgccc
     2521 acctggcgct ccttcatgat cttcagataa ccttccacgg cgttgatcgg tgatttgagc
     2581 tcatgcgaca gcaccgagag gaactggaac ctcacctgct tgccttcacg gttcatattc
     2641 cgggtcatcc gtttcaggta gagatgcttg gtgatccctt cgatcgacga tttgagctca
     2701 tccggcgtaa acggcttcgg catgaaattg tatgctcctg tgttcgtggc ctttatagcc
     2761 agatcaagtg aggcatagga ggtgatcatc atcacctggg catcatattc cttctttttg
     2821 atataatcca gcacttcgat gccgtccatg cccggcaact tattgtcgag caggataata
     2881 tccatcggtt gggcctcgat gatctcgata gccctttccc cggtttcggc ttcgatcact
     2941 tcaaaatcga agtcctcatc cataaacgga taccctaccc tgaaattccg catgatccgg
     3001 attatccccg aacggatgcc gggttcgtcg tcgactacaa gaactttaag agttgccatt
     3061 gagttagtca ttttaaccct ccccctttag ttccccctcc ctgatatcag ggagggggat
     3121 agggggtggg ttcatcatat cttcaacagt ttatttatct cccgttccaa ctggtccggg
     3181 cggattcctt tttcgataaa tgtatcggcc ttgatccagg aacggtcctg tgccgtttcg
     3241 acgccaaagc tcaggccggt ctcggctgtg acagccgaag caatgatgac cggcatggcc
     3301 ggatttttct gtttcatctt gtaacacagg ataaatccgc tgtctttgtt ctccatcatc
     3361 agatcgatga tagccaggtc aaattttcct ttaccgatca gttcttcgcc ttccttctga
     3421 gtatctgccg tcaccacatc gtatcccagc cttttaacct gcagttccat ctgtgataaa
     3481 taatcgatgt cgtcatccac gatcaatatt gtcttcttac tttctttcat ttctctttac
     3541 tattttgatt aaatctttta tttccctcca catgccccct ccccgttttg gggagggcat
     3601 tggggtgggg cttttatctt ggtatcttaa ttgtaaatgt cgttcctgtc ggtcctttcc
     3661 acgggtcggc attggatgtc acctggacct ggccacggtg catcttcacg attccgtata
     3721 tgagcggaag tccgagccct gttccttttc ctacttcctt ggtagtgaag aacggggtaa
     3781 atatcttttc catgttttcc tccgggattc cgataccggt atcactgata gcgagctcca
     3841 cctcatgatc tgtgccggtg agcgagatct tcagggtgcc gcctttcggc atggcctcca
     3901 ccgcattttt ttccaggttg gtcagcacct gcatcatctg gtccttgtca atccgggcaa
     3961 tcggatcgct gagcttattt tcgatcttaa tgtcgatgtt atccggccgg atgatcgaat
     4021 cgacactcct tttcacgaag tcgaccgtat ttgtttcgac caggaaaacc tggttcttac
     4081 gggcgaaatt cagcaacccg ccgacaattc cgcggcagcg ttccgcctgc tccacgatca
     4141 gttgcagatc cttgtataca gggctgtcgg gcggcgtttc atcctttaaa atattcgtat
     4201 acatcgtaat aacgcccaaa ggattgttga gttcgtgggc gataccggcc gaaagctgtc
     4261 ccatgctggc cagcttctcc gactggcgga gcgcttgttg ggttgtggcc agttgttcgt
     4321 tcgatttatt cagatctttt aatgaagtat ggagcttctc gatagcatag ggcaggcaca
     4381 tttcgttctc tgccaggccc ttcacgatag cgatggcatg atctacacag gtatcgtatc
     4441 cgcaggcgcc gcaattaagc tgatctttct ccgaaacttt acccatcttg aacaggactt
     4501 catcgacttt ctctttgggc gcttgtagcg gagtccgctc tttccggctg aattgctgag
     4561 aaaaatctat tccggagaat tcctcaacct ggttttccca tttctcttca tccaggtttt
     4621 gcattttctc cctgacatat tcactgacca gggccatccc ttcaaacttc ttgcatccat
     4681 aagtcatccc tgggcccata atgcatcctt cacagcacaa cagttccagg tgctgggagt
     4741 tcatcagccc gttttcaaat tctttgatgg cttcccggaa gttaaccctt ccatcggcca
     4801 ccataatatt gccttcaacg aggtcgtcgt ttttattcgc tgtttgcagc aggccacggc
     4861 ttaccgggaa gatagcacct ttgcccgaaa ccggctcatc gaattccgcc ggttgagttt
     4921 gctccggagt aacgttaaac tcctggaaga gcttcctcag ttccctgaag gtgatcgctt
     4981 cgtcgatctc atcagattct gctttcttgg cgatacaggg gccgatgaag acaatcagcg
     5041 gctcttcgcc gtactttttg cggatcacgc ggcacaaagc caccatgggt gatacgatcg
     5101 gcgccagcga cgggaccagg tcggggtgat aattttcgac ataattgacg atggccgggc
     5161 agtcggagga gatataatgc ttcccgccgt ttccacccag caacctgttg tattccatgg
     5221 ccaccatatc ggcgccgaag gatacttcca cgactttttc aaatcccagc tttttaatca
     5281 tgccgaccag tacccggtaa tctttgattt ccctgaattc ggcagggaaa ctgggggcga
     5341 tgaccgcgta aaccggccgg tcgcctttca ggaagatctt cacctgttcc ctggaatcga
     5401 tgaatacttt ggcatcctgg ctgcaaaccc tgacacaatt cccgcaggcg atgcaccttt
     5461 cggcgatgac ctccgcctgg ccgttaaaaa tacggatggc tttcaccggg cactccctga
     5521 cgc
//
LOCUS       DAOQXS010000147         5464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15216_length_5464_cov_3.860972, whole genome shotgun sequence.
ACCESSION   DAOQXS010000147 DAOQXS010000000
VERSION     DAOQXS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15216_length_5464_cov_3.860972"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..3120
                     /locus_tag="PKM34_05375"
     CDS             166..3120
                     /locus_tag="PKM34_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ83054.1"
                     /translation="MSPDSLNTSLEALRRSLEGDLFTDESYRILHATDASAYREMPIA
                     VARPRTKEDIRKLVTFACENGTSLIPRTAGTSLAGQVVGNGIVVDVSKYLNNILEINA
                     EERWVRVEPGVVLDELNMAVARFSLFFGPETSTSNRCMIGGMVGNNACGAHSIIYGST
                     RDHLISLKAILSDGSDAEFKPITPEEFERRCDGDSLEAAIYRNIRELLKEPGVQEEIR
                     REFPDPKIKRRNTGYAIDQLLEYYPFSANGQPFNFCKILAGSEGTLAFSTEIKLNLVP
                     LPPKQKILLCAHFKTLEEALQGNLVALKHNPGAVELMDKFILDCTRDNIEQRKNRFFI
                     EGDPGAILIIEFARETREELEAITSKITEDFRSAGLGYHYPAVTGADIPRVWALRKAG
                     LGVLTNIPGDAKPVSVIEDTAVNPENLPQYIAEFKEILAGHGLECVYHAHIATGELHL
                     RPVLNLKDPADVKKFRAIATDTARLVKKFRGSFSGEHGDGRVRGEFIPLMIGEKNYEI
                     LRKIKQAWDPGNIFNPGKITGTAPMDTSLRYEPGRATRQFKTVFDFSHDGGILRAAEK
                     CNGSGDCLKSHLIGGTMCPSYMASKDENATTRARANILREFLTRSDRKNPFSHKEIYD
                     VMDLCLSCKGCKSECPSNVDIARYKAEFLQHYYDEHGVPLRSLAVANITRLNRLGAIL
                     PGVYNFMVRNQTISGLMKKVLGMAPQRPLPALHPYTLNTWLNKYNQILPSGLPQRQVW
                     LFIDEFTEFNDVPVGIATVKLLNALGYKVLTCQHPESGRTYLSKGLVRAARKYAIDNV
                     MTFKDKVSEDVPLIGIEPSAILSFRDEYPDLVGPELKDFATKLSKNCLMFDEFFMREI
                     KSGNISKDHFTRSKANIKLHGHCHQKALSTTGPTKEMLSFPENYTVEEIPSGCCGMAG
                     SFGYEKEHYELSMKVGELVLFPAVRQSDQTFIIAAPGTSCRHQIKDGTGREALHPVEV
                     MWSAIDPSLRPG"
     gene            complement(3171..4082)
                     /locus_tag="PKM34_05380"
     CDS             complement(3171..4082)
                     /locus_tag="PKM34_05380"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNQ83055.1"
                     /translation="MSFTVTILGSGAAIPTGERNPTAQVVKVRNRTLLLDCGEGTQVQ
                     LRKAGLRIQKINYIFISHLHGDHYFGLIGLINTMHILGRKDTLHIFAIEPLKRLIDFQ
                     LELSNTTLVYPLVFHPIDPEIHRTIYEDEQISVSTIPMNHRVPTCGFLIREKSGLRRI
                     RKDFARSVKVPVEYYERIRRGEDFTDENGKHYPNHSITDESTAPKSYAYCTDTAYHEP
                     VIPIIRDCDLLYHEATFMEDKSADAKAKFHTTAREAATIALKSNAKKLVIGHFSARYK
                     DSDGLLREAQEVFPETMIAEEGKTFQV"
     gene            complement(4161..>5464)
                     /locus_tag="PKM34_05385"
     CDS             complement(4161..>5464)
                     /locus_tag="PKM34_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ83056.1"
                     /translation="YGVFIDLGGVDGLIHITDLSWGRINHPEEIVELDQKIKVVILDF
                     DDEKKRIALGLKQLMPHPWESLDENLKIGDKVKGKVVVIADYGAFIEIAAGVEGLIHV
                     SEMSWSQHLRTAHDFLKVGDEVEAIILTLDREERKMSLGMKQLVPDPWENIVQKYPIN
                     SKHTATVRNFTNFGIFVELEEGVDGLIHISDLSWSKKIKHPAEFTKIGEQIEVIVLDV
                     DTENRRLSLGHKQLEENPWDVFESVFTLGSVHQGTVVSANDKGFIVSLPYGVEGFVPT
                     RHAAKADNSMAKVDDNLDFKVIEFSKENKKIILSHARVHQDEARAERDKEDGKVKREQ
                     RTTNKAVKKVQDNVEKTTLGDLEALVSLKTDMEKQEKLENKEKLEKLEKKAKAEKAAK
                     AEKEATAEKPEKEEKKSKGKAKSEPKAEEAPEIKFDAEPEA"
BASE COUNT         1285 a         1389 c         1406 g         1384 t
ORIGIN      
        1 aggcgatttc gaaattcccg gttgctatta tgtatttgcc cgccgatatc cggatgagaa
       61 cgggaaactt tattcgggat ttatcgccaa atcggccgat aaaatctttg aaagcaccaa
      121 tttctataag aagtaaagca gaaaaaaggg ttaattttgg ctcccatgag cccggattca
      181 ttaaatacct cactcgaagc gctgcgcaga tcgctggaag gcgacctttt caccgacgaa
      241 tcctaccgta ttttgcatgc caccgatgcc tcggcatacc gtgaaatgcc cattgcagtt
      301 gcccgtccaa gaacaaagga ggatatccgc aaactggtaa cttttgcctg cgagaacggc
      361 acatcgctta ttccgcgtac ggccggcacc tccctggctg gccaggttgt gggtaacgga
      421 attgtggtgg atgtatcgaa atatctcaat aacatcctgg agatcaatgc ggaagagcgc
      481 tgggtgagag ttgagccggg tgtcgtgctg gatgaattga acatggctgt tgcccgtttt
      541 agcctcttct tcggtcccga aacttccact tcaaaccggt gcatgatcgg gggaatggtc
      601 ggcaacaacg cctgcggggc gcattccatt atctatggaa gcacacgtga ccatctgatc
      661 tcgcttaagg ccattctgag cgacgggtcc gacgcggaat tcaaacctat tacgccggag
      721 gaatttgaac ggagatgtga tggggattcc cttgaagctg caatctaccg gaatatccgt
      781 gaattgctta aggagcccgg cgttcaggaa gagatccgca gggaatttcc cgatccgaag
      841 ataaaaaggc gaaataccgg gtatgccatt gaccagttgc ttgaatatta cccatttagt
      901 gctaacggtc agccatttaa cttctgcaaa atactggccg gttcggaagg cacgctggca
      961 ttttcgacag aaatcaaact gaatctcgtt ccgctccccc cgaaacaaaa gattctgctc
     1021 tgtgctcatt tcaaaacgct cgaggaggcg ctccagggaa acctggttgc gctgaaacac
     1081 aatcccggcg cagtggaact gatggacaag ttcattctcg actgcacccg cgataatatt
     1141 gagcagcgca agaaccggtt ttttattgaa ggtgatccgg gcgccatcct gatcattgag
     1201 tttgcgcgag agacccggga ggaattggag gctatcacca gcaaaattac agaggatttc
     1261 cggtctgccg gcctgggcta tcactacccg gccgttacgg gtgccgatat tcccagggta
     1321 tgggcccttc gcaaagccgg gcttggagtt ctgacaaata tacccggcga tgccaaaccg
     1381 gtatcggtca tagaagacac agcggtgaac cccgaaaatc taccgcagta cattgccgaa
     1441 ttcaaagaga ttctggccgg gcacgggctg gaatgcgtgt accatgcgca tatcgccacg
     1501 ggcgaactgc acctgaggcc tgtgctgaac ctgaaagatc cggcagatgt gaagaaattc
     1561 cgggctattg ccaccgacac agcacggctg gttaagaaat tccggggttc attcagcggc
     1621 gagcacggcg acggaagggt caggggcgaa ttcatcccgc tgatgatcgg ggaaaagaat
     1681 tatgagatcc tccggaagat caagcaggcc tgggatcccg gcaatatctt caatcccggc
     1741 aagatcaccg ggactgcgcc gatggacacc agcctgcgct atgagccggg ccgggccacg
     1801 cggcaattta agacggtttt cgatttttcg cacgacggcg gtatcctgcg ggctgccgag
     1861 aaatgcaacg gctcgggcga ctgcctcaaa tcgcacctga tcggcggaac tatgtgcccg
     1921 agttatatgg caagcaaaga tgaaaatgcc accacccggg ccagggccaa tatcctgcgc
     1981 gaattcctga cacgctccga tcggaagaat cccttcagcc ataaagagat ttacgatgtt
     2041 atggatcttt gcctttcgtg caagggatgt aagtcagaat gcccgtcgaa tgtagatatt
     2101 gccaggtata aagccgaatt cctgcagcat tattacgatg agcacggcgt gccgctgcgg
     2161 tcgctggcag tagctaacat tacccggctg aaccggctgg gagctatctt gcccggcgtt
     2221 tacaatttca tggtccggaa ccaaacgatc tcaggcctga tgaagaaagt cctcgggatg
     2281 gctccccaac gacccctacc cgctttacac ccttacaccc ttaacacctg gctaaacaag
     2341 tataaccaga tcttgccttc cggtctgcct cagcggcagg tctggctctt catcgacgag
     2401 ttcaccgaat tcaacgatgt gccggtaggc atcgccacag tcaaattgct caatgcactg
     2461 ggctacaagg ttctgacctg ccagcatccg gaaagcgggc ggacctatct ttcaaaaggt
     2521 ttagttcggg ctgccaggaa atatgccatt gataatgtga tgacttttaa ggataaagtg
     2581 agtgaggatg ttccgctgat cgggattgag ccttctgcca ttttatcttt ccgcgacgaa
     2641 tatcccgacc tggtcgggcc ggaattgaaa gatttcgcca ccaaattatc gaagaactgc
     2701 ctgatgttcg acgagttctt catgagagaa ataaaatcgg ggaacattag caaggaccat
     2761 ttcacaagat caaaagccaa tatcaaattg cacgggcact gccatcagaa agccctgtcc
     2821 accaccggtc cgacaaagga gatgctttca ttccctgaga attacactgt ggaagagata
     2881 ccatcgggat gttgcggaat ggcgggctcg ttcggttacg aaaaggagca ttatgaatta
     2941 tcgatgaagg tcggtgaatt ggtattgttc ccggcagtaa ggcaatcaga tcaaactttt
     3001 attattgctg cgcccggcac cagttgccgc caccagatca aagacggaac gggaagggaa
     3061 gctttgcatc cggtggaagt gatgtggtcg gcaatcgatc cctcgctacg cccgggatga
     3121 cggtttggag atttgaagtg cagctctata tggaggcata tacaatatgc tcacacctgg
     3181 aatgtcttgc cttcttcagc gatcattgtc tccggaaaaa cttcctgtgc ttctcgcagc
     3241 agcccatccg aatctttata acgggctgag aaatgcccga taaccagctt tttagcattg
     3301 gatttcaatg cgatcgttgc ggcttcgcgc gctgtcgtat gaaatttggc cttcgcatcg
     3361 gccgacttgt cttccataaa tgttgcctca tgatataaca ggtcacaatc acggatgatg
     3421 ggaatgaccg gctcgtggta ggcagtatcg gtgcaataag cgtaagattt cggagctgtt
     3481 gattcgtctg taatcgaatg attagggtag tgttttccgt tttcatccgt aaaatcctct
     3541 ccccttctga tccgttcata gtattcgaca ggaaccttga cactcctggc gaaatctttc
     3601 ctgatccttc gcagaccact cttttcccgg atcagaaatc cgcaggtcgg cacccggtgg
     3661 ttcatcggga tcgtggagac gctgatctgc tcatcttcat aaatagtccg gtgaatttca
     3721 gggtcaatcg gatgaaaaac aaggggatag accagcgttg tattggaaag ctcaagctga
     3781 aagtcgatca ggcgtttcag aggttcgatg gcgaaaatat gcaaggtgtc tttccttccc
     3841 aggatatgca tggtgttgat caggccgatc aatccgaaat aatgatcgcc atgaagatga
     3901 gaaataaaga tataattgat tttctggatc cgcagaccgg cttttctcaa ctgcacctgt
     3961 gtcccctctc cgcaatccag cagcaaagtc cggttgcgga ctttcacaac ctgtgcggtc
     4021 ggattccttt cacccgtagg gattgcagct cctgatccca gtattgtcac ggtaaaactc
     4081 atgattcaaa agtagttatt ttataaaaaa aaagagcgcc ccggaaatcg aagcgctctg
     4141 ttatggggat aatttcggga ttaagcttcg ggttcggcgt caaatttgat ctccggcgcc
     4201 tcttcagcct tgggttcgga tttagcttta cctttggatt ttttctcctc tttttccggt
     4261 ttttcggctg tagcctcttt ttcagcttta gctgcttttt cggctttggc tttcttctcg
     4321 agtttttcca gcttctcctt attctccagc ttctcctgtt tttccatgtc ggtcttcagc
     4381 gaaacaaggg cttcaaggtc gccgagggtg gtcttttcga cattgtcctg gactttcttc
     4441 acagccttgt tggttgtacg ctgctcccgc ttcactttgc cgtcttcctt gtcgcgttct
     4501 gccctggctt catcctggtg gacgcgtgca tggctgagga tgatcttttt gttttccttg
     4561 gagaattcaa tgaccttgaa atcaaggtta tcatcgactt ttgccattga attatcggct
     4621 ttggcagcat gtcgggtcgg tacgaatcct tcaacaccgt aaggcaggga aacgatgaat
     4681 cctttatcgt tcgctgaaac gacggttccc tggtgcaccg aaccgagcgt aaacaccgat
     4741 tcgaacacat cccacggatt ttcttccagt tgtttgtggc ctaagctcag gcgcctgttt
     4801 tcggtatcga cgtcgagcac gatcacttcg atctgttcgc cgatcttggt aaattcggcc
     4861 gggtgcttga tcttcttcga ccagctcagg tcggagatgt ggatcaggcc gtcgacgccc
     4921 tcttccagtt cgacaaagat gccgaagttg gtgaagttgc ggacggtagc cgtatgcttg
     4981 gagttgatcg ggtatttctg tacgatgttt tcccacggat cgggcaccag ttgcttcatt
     5041 cccagcgaca tcttccgctc ttcgcggtcg agggtgagga tgatggcttc cacttcgtcg
     5101 ccgactttaa ggaaatcgtg agcggtgcgc aggtgctgcg accacgacat ttcagaaaca
     5161 tggataagtc cttccacgcc ggcagcgatc tcgatgaacg cgccgtaatc ggcgatgacg
     5221 accactttgc ctttcacttt gtcgccgatc ttcaggttct cgtccagcga ttcccacgga
     5281 tgcggcatca actgcttcag gccgagggca atacgcttct tttcatcgtc gaagtcgagg
     5341 ataacgacct tgatcttctg gtcgagttcc acgatctctt ccgggtggtt gatacgtccc
     5401 cacgacaggt cggtgatgtg aataagtccg tccacaccgc ccaggtcgat gaacacaccg
     5461 tacg
//
LOCUS       DAOQXS010000148         5438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15319_length_5438_cov_4.068178, whole genome shotgun sequence.
ACCESSION   DAOQXS010000148 DAOQXS010000000
VERSION     DAOQXS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_5438_cov_4.068178"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="mtaB"
                     /locus_tag="PKM34_05390"
     CDS             complement(<1..1275)
                     /gene="mtaB"
                     /locus_tag="PKM34_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNQ83057.1"
                     /translation="MDNGQWTMTNLPRHRIAFKTLGCRLNQFETDALAAQFRRHGYTI
                     VDFDGDADICIVNTCTVTNQGDAKSKKAISQAIRKQQQPVVVVTGCMVNSQKEMLQTM
                     NGVTYFVENARKTSIFQLVEAHFSGETASPEDFEGDLFAFEPADETFHTRSFIKIQDG
                     CDNFCTYCIVPKVRGRAISRPAADILENIRTVIGFGFKEIVLTGVNIGRYNDHGTDFE
                     NLVEKILELPGDFRLRISSIEPEGFGDKLLELFSHPKMTPHLHLCLQSGSDRILLQMR
                     RFYTLNDFRSLADRIRSRMPDFNLTTDIIVGFPGETDEEFNQTCTVVREIGFSHVHTF
                     KYSTRTGTRAARMPDPVPEKIKQERSLIIRKISDENKRKYRQSMLGKEQCVLVEKYNP
                     KTKLARGYGQHYIPVEFRSEENIHNRFALVMLE"
     gene            complement(1297..2649)
                     /gene="miaB"
                     /locus_tag="PKM34_05395"
     CDS             complement(1297..2649)
                     /gene="miaB"
                     /locus_tag="PKM34_05395"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNQ83058.1"
                     /translation="MNYHLLTLGCQMNLSDGERVRTVIDGMGYQWTESEEDAGLLGII
                     ACSVRQKSIDKVYSRINRWNRRKDRQNLVTFISGCILPADREKFLKLFDFVFPMSELN
                     QFPEMLSQYGVVTPASLKAMAEKMRLNESIHEFWHIPAHYNSAFEAFVPIQNGCDKFC
                     AFCAVPYTRGREISRPSAEILDEVKSLIGRNYKSITLLGQNVNSYGIDKKGEEILFPQ
                     LLREIGEIGKASGKEFWVYFTSPHPRDMSDEVIDVIAAYPNLGKQIHLPLQSGDDKVL
                     IRMNRKHSLDKFRSVMQKTRETLPQATVFTDIIVGFSGETEEQFENTVKAMEEFRFNM
                     AYIAQYSPRPGAASSRWADDISPDLKKERFRKLTGQLQKFSLELNREMIGKTFRTLVT
                     GPDRKEGFMSGLTEGKIVLRFPSGDTSLTGNFIDVVIRSATPFSLEGDRVNPSKYNLK
                     "
     gene            complement(2714..3385)
                     /locus_tag="PKM34_05400"
     CDS             complement(2714..3385)
                     /locus_tag="PKM34_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ83059.1"
                     /translation="MDPNITTEYNTLRVRSIRELTPSAYVLRFERNNIPFRAGQHILL
                     GKKGELQAREYSVYSAEQDDHFEVLIKEVEEGLVSKQLKRLAPGDPVNFESPLGYFIL
                     RDEDIRDRKFLFIASGTGISPFHSFVRSYPGLDYKLLHGVRTGSETYEKQDYDSNRLI
                     VCTSRDKNGDFHGRVTDYLRAHPVEDKNTLCYFCGNCEMIHEAYDIMLQQGIPSENLF
                     AEVYF"
     gene            3505..4737
                     /locus_tag="PKM34_05405"
     CDS             3505..4737
                     /locus_tag="PKM34_05405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ83060.1"
                     /translation="MMTFEEFGLDEKLIEAITYMGYEKPTPIQEAAIPEILKGRDLIA
                     GAQTGTGKTAAFLLPILNKITRRDVHDVNTLVIVPTRELALQIDQQIHGIGYFLPVTS
                     VSIYGGGGTVDWETEKRSLTGGADIIVATPGKLIAHLNMGYVKFETLEHLVLDEADRM
                     LDIGFYDDIMKIVAYLPKKRQTLMFSATMPPKIKELASKILNDPFIISRELAKPPSGV
                     LQGAYLVFENQKSALINSLIEGKPQCKSILIFTSTKSKVGQIVRALKGRGYKVEGISS
                     DLEQQEREEVLLRFRSRQTRVLVATDVMSRGIDIKDIDLVINYDAPHDAEDYVHRVGR
                     TARAESSGIALTLVNPEDMERFHKIENLIGQLVYKIPLPVELGEGPKWEIKPGGSGRG
                     FRRKGAGPGAKNPGKKRH"
     gene            4940..5311
                     /locus_tag="PKM34_05410"
     CDS             4940..5311
                     /locus_tag="PKM34_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83061.1"
                     /translation="MNTQIGNRDKRIALLLVAAFAWLFIGSLVVFHQEHVLGKHFRMN
                     THLFISPKLKDKENYSVALQKSLLKFQETGQMAGIVPGDHAQPGLISYSFSAYKDTYV
                     FRRLHDIVVTNAPLRAPPVTC"
BASE COUNT         1296 a         1268 c         1376 g         1498 t
ORIGIN      
        1 ttcgagcatg acaagagcaa aacggttgtg gatattttct tcagagcgga attcgacagg
       61 aatgtaatgc tggccatatc ccctggccag ctttgttttc gggttgtatt tttccaccag
      121 cacacattgc tcttttccca gcatcgattg ccggtattta cgcttatttt cgtctgaaat
      181 cttccgtatg atgaggctcc gttcctgctt tattttttcg gggaccgggt cgggcatacg
      241 ggcggcgcgg gtgccggtac gggtgctgta tttgaatgta tgcacatgcg agaagccaat
      301 ttctcttaca accgtgcagg tttggttgaa ttcttcatcg gtttcgccgg gaaagcctac
      361 aataatatcg gttgtcaggt tgaaatcggg catccgggac ctgatcctgt cggccaggga
      421 tcgaaaatca tttaacgtat aaaagcgccg catttgcaac aggatcctgt cggaaccgct
      481 ctgaaggcaa agatgaaggt gcggggtcat tttcggatgg ctgaataatt ccaggagttt
      541 atcgccgaat ccttccggtt cgatggaaga aattcgcagc cggaaatcac cgggcagttc
      601 cagaatcttt tccaccaggt tctcgaaatc ggtaccatgg tcattgtatc ggccgatgtt
      661 gacgccggtc agtacgatct ctttgaagcc gaaaccgatg accgtacgga tattttccag
      721 gatgtcggct gccggcctgc tgatagcccg gcccctgacc tttggaacaa tgcaatacgt
      781 acagaagtta tcacagccat cctggatttt gataaagcta cgggtatgaa aggtctcatc
      841 ggcaggttca aaggcgaaca aatctccttc aaagtcttcg gggcttgccg tttcgccgct
      901 gaaatgggct tcgaccagct ggaatattga ggtcttcctg gcattttcca caaagtaggt
      961 caccccgttc atagtctgca gcatttcttt ttggctgttg accatacatc cggtaacgac
     1021 gacgaccggt tgctgctgtt tcctgattgc ctggctgatg gctttctttg acttggcatc
     1081 accctggttg gtgacggtgc aggtgttgac gatacagata tcggcatccc cgtcaaaatc
     1141 cacgatggtg taaccatggc ggcgaaactg ggcggccagt gcgtcggtct cgaactggtt
     1201 cagtctgcaa cccagggttt taaaggcgat acggtggcgg gggaggttag tcattgtcca
     1261 ttgtccattg tccattttag tggattattg gttattttat ttgagattgt atttagaagg
     1321 attgacccga tctccttcta atgaaaaagg tgtggcggag cggatcacga cgtcgatgaa
     1381 gtttccggta agggaggtgt cgccggaagg aaatctgagg acgatttttc cttctgtcag
     1441 gccggacata aaaccttctt tccggtcggg gccggtaacc agggttcgga atgttttccc
     1501 gatcatttcc cggttcagtt ccagggagaa tttctgcaac tggccggtca gtttcctgaa
     1561 gcgttctttt ttcagatcag gcgaaatatc gtcggcccac cgggagctgg cggctcccgg
     1621 acgcggggag tactgggcga tatacgccat attgaaccgg aattcctcca tggccttcac
     1681 cgtattttcg aattgctctt ccgtttcacc ggagaaaccg acgataatat cggtgaatac
     1741 ggttgcctgc ggcagggttt cgcgtgtttt ttgcattacc gacctgaatt tatcgagtga
     1801 atgtttgcgg ttcatccgga tgagtacttt gtcgtcgccc gattgcaggg ggaggtgaat
     1861 ctgtttcccc aggttcggat aagcggcaat gacgtcgatc acttcatccg acatatccct
     1921 gggatgaggc gatgtaaagt acacccagaa ttcctttccg gaggcctttc cgatttcacc
     1981 gatttcacgc aataattgcg ggaataatat ttcttctcct ttcttgtcga tgccatagga
     2041 attgacattc tgccccagca gggtgatgga tttataattc ctgccgatca ggcttttgac
     2101 ctcatcgagt atttcggccg atggccttga aatttcgcgg cctctcgtgt atgggacagc
     2161 acagaaagcg cagaatttgt cgcatccgtt ctgaatggga acgaaagcct cgaaggcgga
     2221 gttgtaatga gcagggatat gccagaattc atgtatgctt tcattgagcc tcattttttc
     2281 ggccatggct ttcagcgagg cgggggtgac cactccgtac tggctgagca tttccgggaa
     2341 ctggttgagt tcgctcatag ggaatacgaa atcaaacagt ttcaggaact tctccctgtc
     2401 ggccggcagg atacatccgg agatgaaagt aaccaggttt tgacggtctt tcctccggtt
     2461 ccatcgattg atccgggaat agactttatc gatcgatttt tgccttactg aacaggctat
     2521 gatgcccagc agacccgcat cttcttccga ttcggtccac tggtatccca tcccgtcgat
     2581 gacggttcgc accctttccc cgtcggagag gttcatctgg cagccgagag ttagtaaatg
     2641 atagttcaaa gttcagcgtt caaagttatg cgtgagagtt acgggtatta ttttttaata
     2701 gctgatggga ttcctaaaaa tatacttctg cgaacaggtt ttcggaaggg atgccctgct
     2761 gcagcatgat gtcataggct tcgtgtatca tttcgcagtt tccgcaaaaa tagcacaacg
     2821 tatttttatc ttctacgggg tgagccctca ggtagtcggt cacccggcca tggaaatcgc
     2881 cgttcttatc ccgggaggtg caaacaatca gccggttgct atcataatcc tgtttttcat
     2941 aagtttcgct cccagtcctt accccgtgaa gcaatttgta atcaagtccc ggatacgatc
     3001 tgacgaaact gtggaaaggg gagatgccgg taccgctggc tataaataag aatttccggt
     3061 cgcggatgtc ttcatctctc aggataaaat aacccaaagg actttcaaag ttgaccggat
     3121 cgcccggggc caatcttttt aattgttttg aaacaaggcc ctcttccact tccttgatca
     3181 ggacttcgaa atgatcatcc tgctcggcgc tgtaaacact gtattcacgg gcctgaagtt
     3241 ctcctttttt tccgaggagg atatgctggc cggcccggaa agggatattg ttccgttcga
     3301 accggaggac ataggcagaa ggtgtaagct cacggatact cctgacccgg agcgtgttat
     3361 attctgtagt tatatttgga tccactttat ttatcgctta ttttaattta atcttaataa
     3421 tttgcaaaga aacaaatttt ccggtttatg ccgttttcgt cgagggtcgg attaatcctt
     3481 acctttgccg gaaaatgaat attgatgatg acttttgaag aattcggcct ggatgagaaa
     3541 ctgattgagg ctattacgta catgggttac gagaagccga cgcccatcca ggaggctgcc
     3601 attcccgaaa tcctgaaagg gcgtgacctt atagccggcg ctcagacggg gacgggaaag
     3661 acggccgcat ttcttcttcc catccttaac aaaattaccc gccgggatgt tcacgatgtg
     3721 aatacattgg tgattgttcc tacacgtgaa ctggcgttac agatcgatca gcagatccat
     3781 ggcatcgggt attttctgcc cgtcacttcc gtttcgatct acggaggggg cgggacggtc
     3841 gactgggaga cggagaaacg ctctttgacc ggcggcgccg acattatcgt ggccaccccg
     3901 ggcaagctga ttgctcacct gaatatgggc tacgtaaaat tcgagacgct ggaacatctt
     3961 gtgctcgacg aagccgacag gatgctggat atcgggtttt atgacgatat catgaaaatc
     4021 gttgcctacc ttccgaagaa aaggcagacg ctgatgttca gcgccactat gcctcccaag
     4081 ataaaagaac ttgcctccaa aatcctgaac gatccattca ttatttcgcg tgaactggcc
     4141 aaaccgccat caggcgtatt acagggggcc tacctggtat tcgaaaacca gaaatcggcg
     4201 ctcattaaca gcctgatcga aggcaaaccg caatgcaaaa gcatcctcat ctttacctct
     4261 accaagagca aggtagggca gatcgtcagg gccctgaagg gcagaggata caaagtggaa
     4321 gggatttctt cggatctgga gcagcaggag agggaagagg tgttgctccg cttccggagc
     4381 cgccagaccc gcgtgcttgt agccactgat gtgatgagcc gcgggatcga tatcaaggat
     4441 atcgacctgg tgattaatta tgatgcgccg catgatgccg aagattatgt gcaccgcgta
     4501 ggccggaccg cccgggccga gtcttcagga atcgcattga cactggttaa tccggaggac
     4561 atggaacggt ttcataagat tgagaatttg atcggccaac tggtttataa aattccattg
     4621 ccggttgagt tgggtgaagg ccctaaatgg gagataaagc ccggcgggag cggcaggggt
     4681 tttcgccgga aaggcgccgg gccgggagct aaaaacccgg gaaagaagag gcactaaacc
     4741 cgtgatgttg ccactcaatt ttgttgaaac tgctcttgag gattacgatg aaaatgctat
     4801 catcttatct gaattcttta taaattatac ttataaatat ttgaataaaa gataattata
     4861 aattctctat catttattca aacattcaca taatttcata tcgaattgct tattattttt
     4921 gcaccgaatt ttaattgata tgaatacaca gatcgggaac agggataaaa ggattgcgct
     4981 gctgctggtt gctgcatttg cgtggctttt tatcggttcc ctggtcgttt tccaccagga
     5041 gcatgtcctg ggaaaacatt tcaggatgaa cacccatttg ttcatctctc cgaagctgaa
     5101 agacaaggaa aattactccg tcgcgcttca gaaatcattg ttaaagttcc aggaaaccgg
     5161 ccagatggcc ggtatcgtac ccggagatca tgcccaaccg ggactcatat cctattcatt
     5221 ttcagcatac aaggatactt atgttttcag gcgtttgcat gatattgtcg tgacgaacgc
     5281 cccgcttagg gcgcccccgg ttacctgcta atcaggatgt ggtctgtcgg gccacctctt
     5341 ttccttcatt ttctttcatt catttttcct ctgtaattta ttagcattca atgtttcaca
     5401 atctaatcat atcacgcatg aaatattttt cactaatc
//
LOCUS       DAOQXS010000149         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15330_length_5436_cov_3.283962, whole genome shotgun sequence.
ACCESSION   DAOQXS010000149 DAOQXS010000000
VERSION     DAOQXS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_5436_cov_3.283962"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1050
                     /locus_tag="PKM34_05415"
     CDS             388..1050
                     /locus_tag="PKM34_05415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNQ83062.1"
                     /translation="MKEQYFSLQGLQKETEEMILEITSGRLKSNPRKWNADISRSALI
                     VTDMQNYFLNPDSHAFIPSATSIIPNILSLADFFHKSNRPVIFTKHTNDHENAACMSY
                     WWKDMIGNETPSAKIHENLRTSGDIILEKHQYDAFYNTRLEEILHNQNVRFAVICGVM
                     TNMCCETTTRSAFVKGFQPLLPLDATAAYNREFHISTFINLSFGFCPLMTTREVIEVL
                     MK"
     gene            1047..1934
                     /locus_tag="PKM34_05420"
     CDS             1047..1934
                     /locus_tag="PKM34_05420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ83063.1"
                     /translation="MTENKIIIIGYGPSGIACAIQMVRLGLHPVVIEKSRPGGMLTNA
                     RLIENYPGFPEGITGEKLARRMIKQAKRFNINVIKDEITFVDHRNDNYILKGIIENYT
                     CDVLVAATGTVPVTPENIPAKLFRNKRIHTDIAGLKNISGKSVGIVGAGDAAFDYSLS
                     LAEKENNVMIFNRGDQVKALKVLKENVFINNRINYIDNIEIKSLEILPDKRLHATCNS
                     ASAIRNYFLDYLIFATGRKPADGFFQKSLEGKLDGLISSHRLYLIGDLKNGSCRQVSA
                     AVGDGVRAAMEIFHHESNQ"
     gene            1918..2853
                     /locus_tag="PKM34_05425"
     CDS             1918..2853
                     /locus_tag="PKM34_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83064.1"
                     /translation="MKVISKTENSQLATVYIAQNSSGKLIEFVESVQPPLTLSEKWVL
                     IVSTLYGCPVDCSFCDAGGNYHGRLTADEILFQVDYPVRLRFPDGVIGTEKFKIQFSR
                     MGEPSFNPAVLTVLEKIPQLYTIKEFIPSLSTIGPNGTDKFFGRLLEIKKELYPKTFQ
                     FQFSIHTTSLKQRDQLIPVRKWDFRQMARYGEKFFDEEGKKITLNFAVSRESIIDPDV
                     LTRHFSPDIFLIKLTPVNPTFKARKNQIESLDLKDEGFYSDVIQNIREAGYETIVSIG
                     EMEENLIGSNCGQYVQAHLKSCEKMPEAYQYQLNQ"
     gene            3189..3641
                     /gene="tnpA"
                     /locus_tag="PKM34_05430"
     CDS             3189..3641
                     /gene="tnpA"
                     /locus_tag="PKM34_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNQ83065.1"
                     /translation="MASTYISVNIHYVFSTKGRKNLISKEIKPRLWQFMGGIARKNKM
                     VAYAVGGTENHVHVLLSIPATINIAEAIKLIKGGSSKWVNDEFIVPGGFKWQEGYGAF
                     SVSQRGLQRTIAYILNQEDHHRKKSFRDEYVDFLNQCGIQYDERFLLG"
     gene            3664..>3860
                     /locus_tag="PKM34_05435"
     CDS             3664..>3860
                     /locus_tag="PKM34_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83066.1"
                     /translation="MFLKCPFGTWASANRFTDTFTTDESVSYYQLSLWDICNPEGISD
                     NNPPIYRRGTEPGCTRHVRNG"
     assembly_gap    3861..3960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4172..>5436
                     /locus_tag="PKM34_05440"
     CDS             4172..>5436
                     /locus_tag="PKM34_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ83067.1"
                     /translation="MESLLKDLNEPQKNAVINYEGPSMVLAGAGSGKTRVLTYRVAYL
                     VHKGIDPFHILALTFTNKAAREMKERITSLVGADARNVWMGTFHSIFSRLLRIDGYRL
                     GYTPQFTIYDTEDSKRTIRNIVKEMDLDPKVYQPGYILHRISSAKTRMMSAREYFDDP
                     EIKAADQSSGKPHIGQIFLSYAERLKKSDAMDFDDLLVNMNILLEHYPEVLLKYQRKF
                     EYILVDEYQDTNYCQYLILKHLAANNENICVVGDDAQSIYAFRGASIQNILNFRHDYP
                     DHRIFKLEQNYRSTQNIVNAANSIIINNKDQIEKKIWTQNDTGPKIRLIRAKSDTEEG
                     TLVVNAIFELKMNKQLGNDAFAVLYRTNAQSRSFEESLRKLNIPYRIYGSVSFYQRKE
                     IKDLLAYFRLAINNRDEDALIRVINYPARG"
BASE COUNT         1592 a         1228 c         1168 g         1348 t
ORIGIN      
        1 caatcaattt agcacgtcct tttctgacaa gctgttgaat tgttggcata cgctctttat
       61 taaaatttta atttgctttt tgcttccttt ttttacagac acaatatgtt gtgtctttac
      121 cctctcattg ttcaggattt ttcaggaaag tatacaaggc gggctcttac ggagactgag
      181 cggctttctt tttcacgccc tgaacctata tcgtctcacc ttgtcaggtt cctaagtctt
      241 tctggataag ctcagaacga ctgtccttaa aattcgggct gcaaaggtat aaattgattt
      301 tgaattaaca aacgtttatg gaaatatttt ttttgaggag gaaagcttaa ctctgcgttg
      361 cgaaattaaa aaatatatct ttattccatg aaagaacagt atttctcatt acagggctta
      421 caaaaagaaa ctgaagaaat gatcctggaa ataaccagcg ggcgtttgaa gagcaatccc
      481 cgaaagtgga atgccgatat cagccggtct gcccttattg ttaccgatat gcagaattat
      541 ttcctcaatc ctgattccca cgcttttatc ccctcagcaa catccattat acctaatatc
      601 ctgagcttgg ccgacttttt tcataaaagt aacagaccgg tcatctttac aaaacatact
      661 aatgaccatg aaaatgcggc atgcatgtcg tactggtgga aagacatgat cggaaacgag
      721 actccatctg ctaaaatcca tgaaaacctg cgaacttccg gtgatatcat ccttgaaaaa
      781 catcaatacg atgctttcta caataccagg cttgaagaaa tactgcataa tcaaaacgtg
      841 cggtttgccg tgatttgcgg ggtaatgacc aacatgtgct gtgaaacaac tacccgaagt
      901 gcattcgtaa agggtttcca acccttattg cctttagacg ccacggccgc atacaaccgg
      961 gagtttcaca tttccacttt tataaacctg tccttcggtt tctgcccgct gatgaccacc
     1021 cgggaagtca tagaagttct catgaaatga ctgaaaataa aatcattatc attggttatg
     1081 gtccatctgg cattgcctgt gcaattcaga tggtccggtt gggactccat cccgtggtca
     1141 tcgaaaaaag ccggcccgga ggcatgctca ccaatgcccg cctcatcgaa aactatccgg
     1201 gtttcccgga aggaataacc ggtgaaaaat tggcccggcg tatgatcaag caagccaaac
     1261 ggtttaatat caatgtaata aaagatgaaa ttacctttgt cgatcaccgg aatgataact
     1321 acatattaaa aggtataata gaaaattata catgcgatgt acttgtcgcg gccacaggaa
     1381 cggtaccggt gacacctgag aatataccgg caaagctttt ccgcaacaaa cgaatacata
     1441 ccgatattgc cgggctgaaa aacatttcgg ggaagtcggt cggtatcgtc ggcgccggcg
     1501 acgctgcctt cgattacagc ctctcattag ccgaaaaaga aaataacgta atgatcttca
     1561 accggggaga tcaggtcaag gcattgaaag ttcttaagga aaatgtattt atcaacaata
     1621 ggataaatta cattgacaat attgagataa aaagcctgga aatccttcct gataaacgtt
     1681 tgcatgccac ctgcaacagt gcgtctgcga ttagaaatta tttcctggat tatcttattt
     1741 ttgcgaccgg aagaaaaccg gctgacggtt tctttcaaaa aagcctggaa ggcaagctgg
     1801 acgggctgat aagcagccac cggctctacc tgatcggcga cctgaagaac ggctcttgtc
     1861 gtcaagtctc agcagccgtt ggcgatggag taagagcggc aatggaaata ttccaccatg
     1921 aaagtaatca gtaaaacaga aaacagccaa ctggcaacag tttatattgc gcagaattct
     1981 tcaggaaaac tgatagaatt cgtcgaatct gttcaacccc cccttacact ttcggaaaaa
     2041 tgggtactga ttgtatcaac cctttacgga tgcccggttg attgctcctt ttgcgatgcg
     2101 ggcgggaatt atcacggcag gcttacagcc gacgaaatac tcttccaggt cgattatccg
     2161 gttcggctca gattcccaga tggtgtgatc ggaacggaga aattcaagat ccagttctcc
     2221 cggatgggcg aaccgtcatt taatcccgct gttctgacgg tactggaaaa aatccctcaa
     2281 ctttatacca ttaaagaatt catcccttcc ctttcaacga taggcccgaa tggtaccgat
     2341 aaatttttcg gacggctcct tgagatcaag aaagaattgt accccaaaac ctttcaattc
     2401 cagttttcaa tccacacaac ttcgctgaag caaagggacc agctgattcc ggtaagaaaa
     2461 tgggacttca gacaaatggc caggtacggc gagaagtttt ttgatgaaga aggaaaaaag
     2521 atcaccctca atttcgccgt gtccagggag agtattattg acccagatgt actgacccgt
     2581 catttttccc cggatatctt tctaattaag ctcaccccgg taaacccgac tttcaaggcc
     2641 agaaaaaacc aaatagaatc tttggatctt aaggatgaag gtttttattc tgatgtaatt
     2701 cagaatatca gggaagccgg atacgaaacc attgtgagta tcggcgaaat ggaagagaac
     2761 cttatcggca gcaattgcgg gcaatatgtt caggcgcatt tgaaatcatg cgaaaaaatg
     2821 ccagaagcct accagtatca attaaatcag taaaaaaatt ccaaacgccg gggaatcgat
     2881 accttttttt aagatatccc tccgggacac ctgaaaatcc cgaagggatt tccgatagta
     2941 gtccgccgat ttatcagaga acggacatcg gataatcccg aagggatttc cgataataac
     3001 ccgccgattt atcggagagg cacagaaccc gggatgtacc cgtcatgtcc ggaacggaca
     3061 cttgataatc cgtccggatt tcaatccggt caacaaatcc aaaatatttt tttgaaccat
     3121 cgggtatttt tcaataaaaa accggattaa gcagtataga tcatttctta atctcaaaat
     3181 catacattat ggcatcaacg tatatctcag tgaacattca ctacgtgttc tccacaaaag
     3241 gaagaaagaa tctgatctcg aaggagatca agccccggtt atggcagttt atgggaggaa
     3301 ttgcaaggaa aaataaaatg gttgcatatg cagtaggcgg aacagagaat cacgttcatg
     3361 tgctgctctc aatcccggca acgattaata ttgccgaggc aattaaactg atcaaaggcg
     3421 gatcatcaaa atgggttaac gatgaattta ttgtacccgg aggatttaaa tggcaggagg
     3481 gatacggcgc atttagtgtc agccagaggg ggctgcaaag aaccattgct tatatcctga
     3541 accaggaaga tcatcacagg aagaaatctt tcagggatga atatgtcgat tttttaaacc
     3601 aatgtgggat tcaatatgat gaaaggtttc ttttagggta atgttttctg gattaaaatt
     3661 cttatgtttc tcaaatgtcc cttcgggaca tgggcctcgg cgaatcggtt tacggatacg
     3721 ttcacaaccg atgaatcggt atcctattat caattgtccc tctgggacat ctgcaatccc
     3781 gaagggattt ccgataataa cccgccgatt tatcggagag gcacagaacc cggatgtacc
     3841 cgccatgtcc ggaacggaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 cgatttatcg gagaggcaca gaacccggat gtacccgcca tgtccggaac ggacaccgga
     4021 taatcccgaa gggatttccg atagtaaccg ccgatttatc ggctgtgacg gaccggggat
     4081 actccctttg gaaggagccg gatttactgt ttaaaaatag tcaatttaat ttgaagaaga
     4141 aagcctattt ttgccgcttt aactccgact gatggaatcg ctgctgaaag atctgaacga
     4201 accccaaaaa aatgctgtca tcaattacga agggccatcc atggtcctgg ctggtgccgg
     4261 ctcggggaaa acccgcgtac tgacataccg tgtggcttac ctggttcaca aagggattga
     4321 tccgtttcat atcctggctc ttacctttac caacaaagcc gcccgggaga tgaaagaacg
     4381 tatcacaagc ctggtcggcg ccgatgcccg gaacgtctgg atggggacct tccattccat
     4441 tttctcacgg ctcctccgga tcgacggtta ccggctgggc tatactccac agtttaccat
     4501 ttacgatacg gaagactcca agcgtactat ccgcaacata gtcaaggaga tggacctcga
     4561 tccgaaagtt taccaacccg gctacatcct tcacaggatc tcttcggcaa aaacccggat
     4621 gatgtcggcc agggaatatt tcgacgaccc ggaaattaaa gcagctgacc aatcctccgg
     4681 caaaccgcac atcgggcaga tcttcttaag ctatgccgaa aggctcaaaa aatctgacgc
     4741 catggatttc gatgacctgc tggtcaatat gaatattctg ctggagcatt accctgaggt
     4801 ccttctcaag tatcagcgta aattcgaata cattctcgtg gatgaatacc aggacaccaa
     4861 ctactgccaa tacctgattc ttaaacatct agcagctaac aacgaaaata tttgcgtggt
     4921 tggcgacgac gcgcagagca tatacgcttt ccggggcgcc agtatacaaa atatcctgaa
     4981 tttcaggcat gactatcccg atcacaggat tttcaaactg gagcagaatt accggtcgac
     5041 ccagaatatc gtcaatgcag ctaacagcat catcataaat aataaagacc agatagaaaa
     5101 aaagatctgg acacagaatg atactgggcc gaagatcagg ttaataaggg ccaaaagcga
     5161 cacagaagaa gggacgctcg tagtcaatgc catcttcgag ctaaagatga acaaacagct
     5221 cggaaacgac gcttttgccg ttctttaccg cacgaatgcc cagtcgaggt cgttcgagga
     5281 atcgctgcgg aaactcaata ttccctaccg gatctatggc agtgtctctt tctatcagcg
     5341 caaggaaatc aaagatctcc tggcttattt ccgtctggca atcaacaacc gggatgaaga
     5401 cgcgctgatc cgggtcatta actacccggc ccgggg
//
LOCUS       DAOQXS010000150         5396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15470_length_5396_cov_4.152780, whole genome shotgun sequence.
ACCESSION   DAOQXS010000150 DAOQXS010000000
VERSION     DAOQXS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15470_length_5396_cov_4.152780"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKM34_05445"
     CDS             <1..247
                     /locus_tag="PKM34_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNQ83068.1"
                     /translation="KSMTGHLLGAAGAIEAIATILAIRNDIIPPTINHFTDDPEIDNN
                     LDFTYHTAKKRVVNYALSNTFGFGGHNASLIFKKYRG"
     gene            264..980
                     /gene="rnc"
                     /locus_tag="PKM34_05450"
     CDS             264..980
                     /gene="rnc"
                     /locus_tag="PKM34_05450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNQ83069.1"
                     /translation="MVKFVIVQFKKDKILYTAIKNLLGFYPCNIHLYKVALSHKSAPQ
                     VWLKGQQVNNERLEYLGDAVLSSVVADYLYKKFPYQHEGFLTEMRSRIVSRSRFNKLS
                     LKMGLNKLIFQGNGAYTSSKSIYGDTFEALVGAIYLDKGYNFTRKIIIRRIIDVHLDV
                     DEIEKTDTNFKSRILEYAQREKKPLEFKVLQEIGDGHRKQYMVELYIDGLPVSRGLDY
                     SIKAAEQSAAEKACEELLSE"
     gene            1184..2824
                     /locus_tag="PKM34_05455"
     CDS             1184..2824
                     /locus_tag="PKM34_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ83070.1"
                     /translation="MKNNTPFHLMILLCVFCAFLLPSLLNAYPGQVVKSFDLPGKYCT
                     GITWDGKNIWVSDRKADLLYCIDPDNGKVIRTLPSPGYWPLALAWDGKNLWNADLKGG
                     TDISENYDGIIYRLDPATGHILQTLDAPSASPIGLAYDGRYLWCADDLSDELIRFSPD
                     DGTTITSFKSPASFSTGLAFDGTYLWVCDRSKDEIYMVSPETGRVVLITPSPGPYPAG
                     LCYDGEYLWHIDYQTDKLYKIVARDDEKFLRSKERKGKLIFTHQIKNFGPGKVTDADL
                     HIAIPVDRDNQQLTAAPSYNMKPAEIVTDRWGQKTARFHFEDLGAGETRTVEMTTLAT
                     TWEVRYFIFPDKVGSRGDIPAEIRGKYLEDNEKFQITHPVIRDAVAAVVGDQTNPYWI
                     VRDIFDHINANMYYEMTGGWNTAPTVLARGSGSCSEYTFVFIAMCRAAGIPARYAGSV
                     VVREEDASMDDVFHRWAEVYLPNYGWIPVDPSRGDQSWPRDQANAIGSVSGTLLVTTE
                     SGGGSETLGWTYNSNQFWTTEPKTYVNFENFGDWEIVE"
     gene            2898..4922
                     /locus_tag="PKM34_05460"
     CDS             2898..4922
                     /locus_tag="PKM34_05460"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNQ83071.1"
                     /translation="MSFRVNDMRIGILGFAPLILCFALLFSSCSSDEAVRLDSPDGRN
                     NIVFILREGKPFYRVIRDTRVVIDTSALGFEIQDGLPIGDNLRMEKATMSGEQNRWEP
                     AWGQRNRIREHYGQLQVILKEKTGPGRTVQLEFRAYNDGVAFRYVVPEQEGLAKLEIM
                     KELTEFRFRDDLSAWWIPGDYDNHEFLYRNTPISGIDSANTPLTMESLTDSLCLSLHE
                     AALVNYPEMRLKRSADDPLTLLCDLVPWPDGVKARVEVPFQTPWRTVQLSKTAGELIE
                     SSLILNLNEPTKLEDISWIRPMKYIGIWWGMHIGKYTWTAGPKHGATTENAKRHIDFA
                     SENNIPGVLVEGWNKGWENWADGVEFPMMEAYDDYNLAEVAGYAKNKGVYLIVHNETG
                     ANAEFYETQVDSAFALYERLGVHAIKTGYVGKMRPEGQYHHGQWMVNHQAMIIEKAAK
                     HKIMVDAHETVKATGLERTWPNFMSREWGRGNEYEAWSEGNPPDHTCILPFTRVLAGP
                     FDFTPGITGIYFKDYKPDNRIHTTLAKQLALMVVIYSPLQMAADLIENYEGHPAFRFI
                     REVPVDWDQTKALNGKIGDYVTIARKKNNAWYIGSITDENPRELLVKLDFLDEKVMYE
                     AVVYADGPDADWVSNPLDYVIEKRMVKKDDEYVIRLAPGGGQAITVTPVN"
     gene            4922..5365
                     /locus_tag="PKM34_05465"
     CDS             4922..5365
                     /locus_tag="PKM34_05465"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNQ83072.1"
                     /translation="MGRKIKLTGGSSYDYTTIGIACHMKDYRFAFFMNESLGFHFKRL
                     DDLISGEEAESRGYSFYLYSHPEERRDYYLVSNYHEDGRLIPSEKGADFFLIVNDILP
                     AALKKEITGKIQKVPQALAAYEIPKGKIKNLEIIFLEIEEHLLPG"
BASE COUNT         1346 a         1298 c         1500 g         1252 t
ORIGIN      
        1 caagtcgatg accggtcacc tgctgggcgc tgccggcgcc atcgaagcta ttgccaccat
       61 cctggctatc cggaatgata tcattcctcc gacgatcaac catttcaccg atgatccgga
      121 gattgacaat aaccttgact ttacttatca tacagccaaa aagcgggttg ttaactacgc
      181 cctgagcaat accttcgggt ttggcgggca taacgcctcc cttatcttca aaaagtaccg
      241 gggataacct tcccggcagg gtgatggtaa aatttgttat tgtccagttt aaaaaagaca
      301 aaatccttta tacagcgatt aaaaacctgc tgggattcta tccctgcaac atacatcttt
      361 ataaagtagc cctttcccat aaatcggctc cgcaggtgtg gctgaaaggc cagcaggtaa
      421 acaacgaaag gcttgaatac ctgggcgatg ccgtgctcag ttcggtcgtg gccgattatc
      481 tttacaagaa atttccttac cagcatgaag gttttctgac cgagatgcgt tcgcgcattg
      541 tgagccgcag ccggttcaac aaactctccc tgaagatggg gctgaacaag ctgatattcc
      601 agggcaacgg ggcgtatact tcgagcaagt cgatttacgg cgatacgttt gaagcgctgg
      661 tcggtgccat ttatctcgac aaaggctata atttcacccg caagatcatt atccggagga
      721 tcattgacgt gcacctggat gtggatgaaa ttgagaagac cgacaccaat ttcaagagcc
      781 ggatactgga atatgcccag cgggagaaga agccgctcga attcaaggta ttgcaggaga
      841 ttggggatgg tcaccggaag caatacatgg tagagcttta tatcgacggg ttgccggttt
      901 cgcggggcct ggattattcg atcaaggcgg cggagcaatc ggctgcggag aaagcctgcg
      961 aggaattact ttccgagtga ggaggatgtc tcaaaaggat ttaaccacaa aggacaccaa
     1021 ggaattcaca aaggcgcaca aaggtatgta aattgatatt caactactta gtgttccttt
     1081 gtgatgtact ttgtgcgcct tcgcggttaa taaaaagcct ttggagacag cctcctcgtt
     1141 ttattatttt ccataatttc atcctcgaaa taaattctta gtcatgaaaa ataatactcc
     1201 ctttcatcta atgatcttat tatgtgtttt ctgtgctttt ttactgcctt ctcttcttaa
     1261 tgcatatccc ggccaggtgg tcaaatcatt cgacctgccg gggaaatatt gtaccggtat
     1321 cacctgggac ggaaagaaca tctgggtctc cgaccggaag gccgacctgc tgtattgcat
     1381 cgacccggac aatggcaaag tgatccgcac cctgccatcg ccgggctact ggccactggc
     1441 actggcctgg gacggcaaga acctctggaa cgccgatctg aaggggggaa cggatatctc
     1501 ggagaattac gatggcatca tctaccgcct tgatccggct accggacata ttcttcaaac
     1561 cctcgacgct ccttcggcca gccccatcgg gcttgcttac gacggccggt acctatggtg
     1621 tgccgacgac ctttccgacg aactgatccg gttcagtccc gacgacggaa ccacgatcac
     1681 ctccttcaaa tccccggctt cattctctac gggcctggcc ttcgatggca cttacctctg
     1741 ggtttgcgac cggtcgaaag acgagattta catggtcagc ccggagacag ggagggtggt
     1801 tctgatcacc ccatcgccgg ggccttatcc ggcaggcctc tgctacgacg gggaatatct
     1861 ctggcatatt gattaccaga ccgataaact gtataagatc gtcgccaggg acgatgaaaa
     1921 attccttcgt tcgaaggagc gaaaaggcaa attgatcttt acgcaccaga tcaagaactt
     1981 tggcccggga aaggtgaccg atgcggacct gcacattgcc atcccggtcg accgtgataa
     2041 ccagcagctt accgcggcac catcttacaa tatgaaacca gcggagatcg tcaccgaccg
     2101 ctggggacaa aaaacggccc ggtttcattt cgaagacctg ggcgccggcg agacccggac
     2161 ggttgaaatg acgacgctgg ccaccacctg ggaggtgcgt tattttattt ttcccgacaa
     2221 ggttggttcc aggggagaca tcccggctga aattagaggt aagtacctgg aagacaacga
     2281 gaagtttcag atcacccacc cggtgatccg ggatgccgtt gcggcagttg ttggagacca
     2341 gaccaatcca tactggatcg tacgggatat cttcgatcat atcaatgcca acatgtatta
     2401 tgaaatgacg ggagggtgga atacggcacc gacagttctc gccaggggca gcggctcgtg
     2461 ttcggagtac acctttgttt tcatcgccat gtgccgggca gccggcattc cggccaggta
     2521 tgccggttcg gtggttgtca gggaggagga tgccagcatg gacgatgtct ttcatcgctg
     2581 ggcggaggtt tacctgccga actacggctg gatcccggta gatccgagcc gcggcgacca
     2641 aagctggccc cgcgaccagg ccaatgccat cggcagtgtc agcggtacgc tcctggtgac
     2701 aaccgagagt ggaggcggat cggaaaccct gggctggacc tacaactcca accagttctg
     2761 gaccactgaa ccgaagacct atgtgaattt tgagaatttc ggggattggg agatagttga
     2821 gtgatagtcc aagttccagg ttccaggttt caggtttcag gtttcaggtt tcgggtttcg
     2881 ggttacgaat tttagttatg agttttagag ttaatgatat gagaattggg attttaggat
     2941 ttgcgccatt gattttgtgt tttgctttac ttttctcatc ctgttcttcg gatgaggcgg
     3001 tgaggcttga ttcaccggat ggaagaaata acattgtttt catcctcagg gagggaaagc
     3061 ccttttaccg ggtgatccgt gatacccggg ttgtgatcga cacatcggcg ctgggttttg
     3121 aaatacagga cggactgccg atcggggaca acctcaggat ggagaaagcg accatgtcgg
     3181 gtgaacagaa ccgctgggaa ccggcctggg ggcagcgcaa ccggatcagg gagcattacg
     3241 ggcagcttca ggtgatcctg aaagagaaga ccggacccgg ccggacagtg cagcttgaat
     3301 tcagggcgta caacgacggc gtcgctttcc ggtatgtcgt tcccgagcag gaagggctgg
     3361 ccaaactgga gatcatgaag gagctgaccg aattccgctt cagggatgat ctcagcgcct
     3421 ggtggatccc gggtgattat gacaatcatg aatttcttta ccgcaatacc cccatcagcg
     3481 gaattgattc ggccaatacc ccgctgacga tggaatcgct gaccgacagc ctgtgcctga
     3541 gcctgcacga agcggcgctg gtgaattacc ctgaaatgag gctgaaaagg tctgccgatg
     3601 atcctttaac gctgttgtgc gacctggtgc catggcccga cggggtaaaa gccagggttg
     3661 aagtcccttt tcagacgccc tggagaacgg tgcaactttc aaaaactgcc ggcgaactga
     3721 tcgaatcatc gttgatcctg aacctgaatg aacccacgaa gctggaagac ataagctgga
     3781 tcaggccgat gaagtacatc ggcatctggt gggggatgca tatcggaaag tatacctgga
     3841 cagccggccc gaagcatggg gctaccacgg agaatgcgaa gcggcacatc gattttgcgt
     3901 cagagaacaa catacccggg gtgctggtag agggttggaa caaaggatgg gagaactggg
     3961 ccgacggcgt tgagtttccg atgatggaag cctatgatga ttacaatctt gcggaagtgg
     4021 cgggatatgc aaaaaataag ggcgtttacc tgattgtcca taatgaaacc ggcgccaacg
     4081 ctgaatttta tgagacacag gtggattccg ctttcgctct ttatgagcgg cttggggtcc
     4141 atgccataaa aaccggctat gtaggtaaga tgaggcctga ggggcaatac catcacgggc
     4201 agtggatggt caatcaccag gcgatgatca ttgaaaaagc ggcgaagcac aagatcatgg
     4261 tggatgcgca tgaaacggtg aaggcgaccg ggctggaacg cacctggccg aatttcatga
     4321 gccgcgaatg gggacggggt aacgaatacg aagcgtggag cgaaggcaat cccccggacc
     4381 acacctgtat cctgccgttc acacgggtcc tggccgggcc gttcgatttt acacccggca
     4441 taaccggcat ttattttaaa gattataagc ccgataaccg gattcatacc accctggcga
     4501 agcaactggc attgatggtt gttatttaca gcccgttgca gatggcggcc gatctgatcg
     4561 aaaattacga aggtcatccg gcgttccggt tcatccggga agtgccggtt gactgggacc
     4621 agacgaaggc gcttaacggg aagatcggcg attatgtgac cattgcccgg aaaaagaaca
     4681 atgcctggta catcgggtcg atcaccgatg aaaatccgcg tgagttgctt gtcaaactgg
     4741 acttcctgga tgaaaaggtg atgtatgaag ccgtggtcta tgctgacggt ccggacgccg
     4801 attgggtttc caaccctctg gattacgtca ttgaaaagag gatggtgaaa aaggacgatg
     4861 aatatgtgat ccggctggcc ccgggaggcg ggcaggccat cacggtaaca cctgtaaact
     4921 gatgggcagg aagatcaaac tcaccggtgg cagctcctac gattacacaa cgatagggat
     4981 cgcctgccat atgaaggact accggttcgc cttctttatg aatgaaagcc tgggttttca
     5041 ttttaaaagg ctggatgacc tgatatcggg cgaggaagcc gaatcgcgcg gttattcctt
     5101 ttatctgtac agtcatccgg aagagaggcg ggattattac ctggtttcca attatcatga
     5161 ggatgggcgt ttgatccctt ctgagaaggg ggccgatttt ttcctgatcg tgaacgatat
     5221 tttgcctgcg gctttaaaaa aggagatcac cgggaaaatc cagaaggtac cgcaggcact
     5281 ggctgcctat gaaattccaa agggaaagat caaaaacctg gagattattt tcctggagat
     5341 cgaggaacat ttgttgccgg gataagctgc cggttcattc cgaatttatt tctttt
//
LOCUS       DAOQXS010000151         5382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15527_length_5382_cov_3.755960, whole genome shotgun sequence.
ACCESSION   DAOQXS010000151 DAOQXS010000000
VERSION     DAOQXS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15527_length_5382_cov_3.755960"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKM34_05470"
     CDS             complement(<1..542)
                     /locus_tag="PKM34_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83073.1"
                     /translation="MKTKISALSFTILALLVSLFCSQGLNAQTKAKIENVDFFAESNR
                     IIITYDINGSGSGETFNIWILVKTASGKILSARTTSGDIGKGIQGGPGKRIEWSYSAD
                     NIVIEEDIGIEVLASLEAAAKKTEEVVQPVKEPEKEPAKEPQKKEEPVQQQYTGKKVS
                     MGAALAMSAVLPGLGKTYAK"
     gene            complement(539..1288)
                     /locus_tag="PKM34_05475"
     CDS             complement(539..1288)
                     /locus_tag="PKM34_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83074.1"
                     /translation="MKDDYKIRRLITAAGLFVLAVACLLTSASCKKLDFTRTEPIGQD
                     KWLHYDDGVNYDGIFANSGGNFDIAIRFSASELAPYDGFRISKLKFFPKVGYPAVYYI
                     TIWSGTEPPTLLKVQEISSVIAETWNEVSYDESYYIDASSDMWFGVWITDYPASTYPA
                     GCDNGPAVVGKGDLFSPDDGVTWYSLYTANSELNYNWNLQVYVTNLYAKTVPLAPSDE
                     SGLTKYKTSLRDLAPVEGVMNGVSSQKTDRP"
     gene            complement(1306..3972)
                     /locus_tag="PKM34_05480"
     CDS             complement(1306..3972)
                     /locus_tag="PKM34_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83075.1"
                     /translation="MKPRTNKFLISLILSCIIILPFITKGQTFEEFKKQRSQEYKQYV
                     ENETKKMQELRDRYDAYVKAREQEFADYISKEWESYEAFKGRPVPVEPKPQVIPTYTP
                     PPQRTEEPAAKIPVKVKEIPRPVEQPAARINPVIEKIPPVDYLKSDLEFSFYGSKIML
                     DYDKALVIPPPSPVSEDGIGLYWTKACKANFNGLINQMLQYKSMLGLNDWGYYQLVRE
                     TSDKISMPDPNARNLLTWFLLTRSGYRAKISFAENKTSVIIPSNQTIYGRDYLTISQV
                     NYYFVDPIASNVIQTYARDYPDATRVFDFTIDRALNLGESPLVKNLSFTYNDQPHQVK
                     VTINQNVINFYKDYPLVDMNVYFDAALERLAAESIGEALLPVLTPMTEVDKVNCLLRF
                     VQTAFPYKVDQEQFGREKYFFAEELFFYPYSDCEDRSVLFSYLVSELLGMKVIGLGFD
                     GHVATAVHFSDKAGVNGAYFTFRNEKFIIADPTFINAPAGLAMPQYSASQAEIIEIAK
                     VNYAENTGVDFRELVRKAGGYRGGNQQDMIADENGNVYITGYYNETFQVGAKTLTGNV
                     DTRNAFAAKFNKYGELQWVAAPTGKAVTTGFGITQDEQQNVIVAGSFLGQAHFAGYTV
                     TSKQGKPDVFVAKYDPQGHCVWAGQASLDTIDQQSFLKYVAKFDRTGKHLATDLFLEN
                     VEFSQNGLFTDNDGTCYLAGNLSGTTGFRVPERTFMNGGEVDFPSMIKKESDKLISQK
                     VERNIAAIFAVTSLIRSNGMMIPGQAAQQALDKYQPLFRKQFPELYSMFGKITFMKNG
                     DGIVTLATKDHETIYYDKLKISDGTQIKIVQLQDGNERLDILSGIKVGKMVIWFSLNY
                     IQMDRLTGNLLFDYDSSHMQLNMNLKEDIIGD"
     gene            complement(3969..5009)
                     /locus_tag="PKM34_05485"
     CDS             complement(3969..5009)
                     /locus_tag="PKM34_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83076.1"
                     /translation="MKIILATWITLFLIPVIVHSQVNVTNSVEVDYNYQQYKSVFDEE
                     QGAFGEPAPDPGTPPPILFNTVPGWLQEPMPASAASFQVIGISDPGLDSVTARRQAIF
                     RALYLGSLMNGCRVRNLVDYYSQEKSAHNSELTGKYADYFEFTSFSPHGVDESTVVHQ
                     HLNDNGECLVIIDFPAYSGGNPALAFQTNGMITEFQRNGKYESNSRIDFSLKAGRHDD
                     ADGFRYLFRSINGLVDMESYSGNEMLPQADARYKYTQTVTTEAPRDKQGIKTDRGLWN
                     ALLLAIIKDITVDIRTRSALVKSTADNYTGNSLALDREAVTGKLSFRLNRIILYDINL
                     WAILEQINFTQP"
     gene            complement(5091..>5382)
                     /locus_tag="PKM34_05490"
     CDS             complement(5091..>5382)
                     /locus_tag="PKM34_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83077.1"
                     /translation="NYVKDSEMNNVSETLEKYEGLSREVVNQKLNGIKVICEKQTRTT
                     TNTYKTYVAIELAGDEIASAMNQRLSTDDKLFIDYNYEKFKETFDKEMENMK"
BASE COUNT         1142 a         1356 c         1440 g         1444 t
ORIGIN      
        1 ttcttggcat acgttttccc caggccgggc aaaaccgccg acatggccag cgcggcgccc
       61 atcgagactt tctttcccgt atattgctgt tgaacaggct cttctttctt ctgcggctct
      121 ttcgcaggct ccttttccgg ttccttaacg ggctgtacca cttcttccgt ctttttggca
      181 gcggcttcga gagaagcgag cacctcgatt ccgatatctt cctcaattac aatattatcg
      241 gcagaatagc tccattcgat ccgttttccc ggaccgccct gaataccttt gccgatatcg
      301 ccggaggtgg ttcgtgcgct caagatcttt cccgatgctg ttttcaccag tatccagatg
      361 ttgaaggttt cgccggatcc gctcccgttg atgtcgtagg taatgatgat cctgttgctt
      421 tcggcgaaga aatcgacatt ttcgatcttc gcttttgtct gtgcattcag gccctggctg
      481 cagaacaaac tgaccagcag ggcaagaatc gtaaagctga gggcgcttat ttttgttttc
      541 atgggcggtc ggttttttgt gatgatacac cgttcatgac accttcaacc ggcgccagat
      601 cgcgcaagga tgttttatac ttcgtcaggc cggattcatc cgacggggcc agcggcactg
      661 ttttcgcata caggtttgta acataaacct gcaggttcca gttgtaattc agttccgaat
      721 tggccgtata gagcgaatac catgtcacgc cgtcatccgg tgaaaagagg tcgcctttac
      781 cgacaacagc cgggccattg tcgcatccgg cagggtaggt agaagccggg taatcggtga
      841 tccagacgcc aaaccacata tccgaagaag catctatata ataagattca tcatagctga
      901 cttcgttcca ggtttcggca attacactgc tgatttcctg caccttcaac agtgtaggag
      961 gttcggttcc agaccagatc gtgatgtaat acactgccgg atagccaact ttcggaaaga
     1021 acttcagttt tgaaatcctg aaaccatcgt aaggagccag ttcggatgcg ctgaagcgga
     1081 tagcgatatc gaaattcccg cccgaattgg caaaaatgcc atcgtagttc actccgtcgt
     1141 cataatgaag ccatttatcc tgccctatcg gctcggttcg ggtaaaatcg agctttttgc
     1201 aggaggccga tgtcagaagg catgcaaccg ccagaacgaa caggcctgca gccgtgatga
     1261 gtcgtcgtat tttatagtcg tctttcataa tccggtttaa agtgattaat ctccgataat
     1321 gtcctctttc aggttcatat tgagttgcat atggctgctg tcgtaatcga acagcaggtt
     1381 tcccgtcagc cggtccattt ggatgtaatt caacgagaac cagatgacca ttttcccgac
     1441 tttgatcccg ctgaggatat cgaggcgttc gttgccatcc tgcaactgga cgattttgat
     1501 ctgggtgccg tcggagatct tgagtttgtc gtagtatatc gtttcgtggt ctttggtcgc
     1561 aagggtaaca atcccgtcac cgtttttcat aaaggtgatc tttccgaaca tggaataaag
     1621 ctccggaaac tgtttcctga acaaaggctg gtatttatcg agcgcctgct gggcggcctg
     1681 tcccgggatc atcatcccgt tgctgcggat caggcttgtt acggcaaaga tggcggcaat
     1741 atttctttcc actttctggg agatgagctt gtcactctct ttttttatca ttgatggaaa
     1801 atcgacctct cccccgttca tgaatgtcct ttcgggaacc cggaatcccg tggttccgct
     1861 gaggttcccg gcgagataac aggtgccgtc gttatcggtg aacagcccgt tctgggaaaa
     1921 ctcgacattt tccaggaaga gatcggtggc caggtgtttg ccggtcctgt cgaacttagc
     1981 cacatatttc aggaacgatt gctggtcgat ggtgtcgagg ctggcctggc ctgcccagac
     2041 gcaatgcccc tgcggatcgt atttggcgac gaacacatcg ggttttccct gtttgctggt
     2101 aacggtatag ccggcgaagt gcgcctggcc caggaacgat ccggcgacga tcacgttctg
     2161 ctgctcatcc tgcgtgatgc caaaaccggt cgtaacggct ttccctgtcg gggcggccac
     2221 ccattgcagc tcaccgtatt tgttgaactt ggctgcaaaa gcgttccggg tatcaacatt
     2281 gcccgtgagc gtcttcgccc cgacctggaa tgtttcgttg taatatcccg tgatataaac
     2341 attcccgttt tcatcggcga tcatatcctg ctggttgcca ccgcggtacc cgccggcttt
     2401 gcggaccagt tcccggaaat cgaccccggt attctcggca taattgactt ttgctatttc
     2461 gatgatctca gcctgggaag cggaatactg cggcatggcc aggccggccg gcgcattgat
     2521 gaaggtagga tcggcgatga tgaatttttc gttgcggaac gtaaagtagg cgccattcac
     2581 cccggccttg tcgctgaaat gcacggcggt agccacatgt ccgtcaaatc ccagcccgat
     2641 cactttcatc cccagcagtt cgctaacgag gtaggagaaa agtaccgacc ggtcttcgca
     2701 atcggaatag ggatagaaaa agagttcctc ggcgaagaag tatttttcgc ggccaaactg
     2761 ttcctggtcg actttatagg gaaacgccgt ttgcacgaac cgcagcaggc agttgacctt
     2821 atcgacctcc gtcatcggcg tcaggaccgg caggagcgct tcgccgatcg attcggcggc
     2881 cagccgttcg agcgccgcgt cgaaatagac gttcatatcg accagcgggt aatccttgta
     2941 gaaattgatc acattctggt tgatggtcac tttcacctgg tgaggctggt cgttgtaggt
     3001 aaagctcagg tttttaacca gcggcgattc gcccaggttc agcgcccggt cgatcgtaaa
     3061 gtcgaaaacg cgcgtagcat ccggataatc gcgggcatag gtctggatca cgttgctggc
     3121 gatcggatcg acgaaataat aattcacctg gctgatggtg aggtaatccc ttccgtagat
     3181 ggtctggtta gaagggatga tcacgctggt cttgttttcg gcgaaggata tcttggcccg
     3241 gtaacccgaa cgggtcagca ggaaccaggt gagcaggttg cgggcattcg gatcgggcat
     3301 gctgatcttg tccgaggtct ccctgaccag ctggtagtag ccccagtcgt tcagccccag
     3361 catgcttttg tactgcagca tctggttgat caggccgttg aaattggctt tgcaggcctt
     3421 ggtccagtat agtccgatcc cgtcttcgct caccggggaa ggaggcggaa tgaccagcgc
     3481 tttgtcgtaa tccagcataa ttttcgagcc gtagaaggag aattcgaggt cggatttaag
     3541 ataatcgacc ggcgggatct tctcaatcac cgggttgatg cgggcggcag gttgttcgac
     3601 cggacgcggg atctccttta cctttaccgg gatcttggcg gccggctctt ccgtcctttg
     3661 gggcggaggg gtataggtcg ggattacctg gggtttgggc tcaaccggaa ccggccttcc
     3721 tttgaacgct tcgtaggatt cccactcctt gctgatgtaa tcggcaaatt cctgctccct
     3781 tgcctttaca tacgcatcgt agcggtcgcg cagttcctgc atctttttcg tttcattctc
     3841 gacatactgc ttgtattcct ggctgcgctg ctttttaaac tcttcgaatg tctggccttt
     3901 cgtgatgaac ggcaggatta tgatacacga aaggatcagg gagattaaaa atttatttgt
     3961 tcttggtttc atggctgggt gaaatttatt tgttccaaaa tagcccaaag gttaatatcg
     4021 tataagatga tccggttcag ccggaacgaa agtttgcctg taaccgcttc ccggtcgagg
     4081 gcgagtgaat ttcccgtata gttgtcggcg gtggatttaa ccagggccga gcgggtcctg
     4141 atatccaccg taatgtcttt aatgatggct aaaagaagcg cattccacag gccgcggtcg
     4201 gttttgatcc cctgtttgtc gcggggagct tcggttgtga cggtctgggt gtatttgtac
     4261 cgggcgtcgg cctgcggcag catttcattt ccggaatagg attccatatc caccagaccg
     4321 ttaattgagc ggaagagata ccggaaaccg tctgcatcat catgccggcc tgctttcagc
     4381 gaaaaatcga tcctggaatt gctctcgtat ttaccgtttc tctggaattc ggtgatcatg
     4441 ccgttggttt gaaaggccag tgcgggattt ccgccggaat aagccgggaa gtcgatgatc
     4501 acgaggcact ccccgttgtc attgagatgc tgatgaacga cagtggattc atcgacgcca
     4561 tgcggcgaaa aactggtaaa ctcgaaataa tcggcatact tacccgtaag ttcgctgtta
     4621 tgcgccgatt tttcctgtga ataataatcg accaggtttc ttaccctgca tccgttcatg
     4681 agcgatccga gatagagggc gcggaagatg gcctgccggc gggctgtaac cgaatcgagg
     4741 cccggatcgg agatcccgat cacctggaac gaggctgctg aagccggcat cggttcctgg
     4801 agccacccgg ggacggtgtt gaacaaaatg ggaggaggcg tgccggggtc gggtgccggt
     4861 tcgccgaacg ccccctgttc ttcgtcaaaa acagatttgt attgctggta attatagtcg
     4921 acttcgaccg aattcgtcac attcacctgc gaatgaacga tcacagggat taagaataaa
     4981 gtaatccacg ttgccaggat aattttcatg accggaaaga taaacagggg ccctgcctct
     5041 tccgacgcag tgcccctgcg tgaataataa gaattcctga ggatctccta ctatttcata
     5101 ttttccattt ccttgtcgaa agtttccttg aacttttcat agttgtagtc gatgaaaagc
     5161 ttgtcatccg tggagaggcg ctggttcatc gcggatgcga tctcgtcgcc ggccagctcg
     5221 atggctacgt aggttttgta cgtattggtg gtggtgcggg tttgcttttc gcagatcacc
     5281 ttgatgccgt tgagcttctg gttaacgact tcgcggctga gtccttcgta tttctcgagg
     5341 gtttcgctga cgttgttcat ctcgctgtcc ttgacatagt tg
//
LOCUS       DAOQXS010000152         5360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15618_length_5360_cov_3.599811, whole genome shotgun sequence.
ACCESSION   DAOQXS010000152 DAOQXS010000000
VERSION     DAOQXS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15618_length_5360_cov_3.599811"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PKM34_05495"
     CDS             <1..1331
                     /locus_tag="PKM34_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83078.1"
                     /translation="HCANKDFANICVLPLYNNLKAQVILKGNATSLPQLVTSGFSVNY
                     SVPGNTVSTNKTNFWDYEDQLFGVNLPPNIGLTGVGLSGTMAVATPENDHFFVDGIPV
                     TPYQDNDLVNEDPYQLALVELHDNQNNLIVQAQPVIPVSNEINCVSSGCHSSEAQILN
                     MHEDEGGFDPNDTPILCAECHSSNALGTPGMPGLESLSQVIHEQHGEETNDCYKCHPG
                     PNTQCHRDIMHTMGMVCQDCHGSVDEVGQSIDDGREPWLEEPQCGNTDCHGSNYAEEQ
                     GKLFRQSRGHGGLFCSACHGSPHALYTSENDRDNQQIIALQGYAGILRECSVCHGVTP
                     AFPGPHGYIPTSVDQLEGEVKESLLLSVPQPNPFKERTVISFNIDKTGKTYLDIFDQN
                     GKQMVRLVSENLGKGQYQITFNAPGFAPGVYILNLRNNGRESSQKLVLTR"
     gene            1454..2530
                     /locus_tag="PKM34_05500"
     CDS             1454..2530
                     /locus_tag="PKM34_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83079.1"
                     /translation="MICLLFSCSPGQNPGQDNAGKVNLADSLRYPDEKHLANLRQLTF
                     GGDNAEAYFSFDDKRIAFQSNNAAWNLECDQIFTFELASGDMKEGAPQMISTGMGRTT
                     CSYFMPGDTTILFASTHAGDHACPHVPPPRPDHKYVWPIYDDYDIYVSDLKGNIIDTL
                     THEPGYDAEATVSPKGDKIVFTSLRSGDLELYTMNLDGSDVRQITDELGYDGGAFFSQ
                     DGSMLVFRASRPKTEEDIRVYKELLSVGQVMPTNMEIFVCNADGSNLRQVTNLGKANW
                     APYFHPSMKKVIFSSNHASDRGYQFNLYMINLDGTELEKITYDGVFDAFPMFSFNGKK
                     LIFSSNRNNGGTRATNLFIADWID"
     gene            complement(2549..2797)
                     /locus_tag="PKM34_05505"
     CDS             complement(2549..2797)
                     /locus_tag="PKM34_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNQ83080.1"
                     /translation="MCLAVPGKLISIETDTPLKMGKVDFDGVVTDICLEFLPEANVGD
                     YVLAHVGTAISLLDEEEAMETLRALRELGEIKPDYELQ"
     gene            2915..3421
                     /locus_tag="PKM34_05510"
     CDS             2915..3421
                     /locus_tag="PKM34_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83081.1"
                     /translation="MKKLSFSLAGLVIFLLMINGCDKESSSAGISGKITGHSDCKFSR
                     SSGISYELADTLSCVYYLYNEISGELNIIHVNSGFNCCPGELSCEITISQDTILIREY
                     EKEQACDCNCLYDLYITVKGVYMGNYVIRFNEPYAEGMEELVFKIDLAENISGSYCVA
                     RKNYPWGI"
     gene            3425..3766
                     /gene="hypA"
                     /locus_tag="PKM34_05515"
     CDS             3425..3766
                     /gene="hypA"
                     /locus_tag="PKM34_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNQ83082.1"
                     /translation="MHELSIAMSIVELAGEEAAKAGASEISKIEVEIGSLAGIETDAL
                     LFCWDAAIRDTMACRAALVIHPIDAEAHCLDCGHVFPINDYYSGCPSCQGFQYRITKG
                     RELRIRSLTVE"
     gene            3931..>5360
                     /locus_tag="PKM34_05520"
     CDS             3931..>5360
                     /locus_tag="PKM34_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83083.1"
                     /translation="MKTKRWKKGMVVMIILLAGWHTPARPGIIVTGILDGTLTGGSPK
                     AVEIFVTGTENLNNYEVWRSLNGAPFGSGSGSVASLNGVFTNTFVYLVKTDHVNAFHD
                     VFGNEGIFANVVPMGIINGNGNDGFQVRKKVGSVVIDQVWLEDASYSYEDSYWYRKNG
                     TGPDGGWIPSAWETPGNGALDGLDQDGLRAAVPFGTYASVWLGINSAWNDSVNWSLGY
                     VPTSHTNTVIPDSLTEYPLIFDPPLEPATCLNLSIAENARLTVESSACFTISGNMEIE
                     SPDTTGALQGLILSSDPNPEMSGSLIVKGTAVGKVTVRRYIPKNNNWHLIASPADTQY
                     FQPEFVPVPVDQSFDLYYWNETAEFNSGWINCRDENGGWYPGFETGFHEGKGYLVAYS
                     PATDGNEVRRFYGRIRTGDYSFPLSCSGNAWNLLGNPYTCALDWASAGISKESIAGSA
                     VYLWDPYLNSGQGGYRAHNGTTGVPSG"
BASE COUNT         1411 a         1283 c         1349 g         1317 t
ORIGIN      
        1 tgcattgtgc aaataaggat tttgcaaata tctgtgttct gccgctatat aacaatttaa
       61 aggcccaggt aatccttaaa ggcaatgcta ccagccttcc ccagctggtt acatcaggtt
      121 tcagtgttaa ttattctgtg cctggaaata ctgtttcaac aaataagacg aatttctggg
      181 actacgaaga tcagttgttc ggcgtcaatc tccctcccaa tatcgggctg acaggtgtgg
      241 ggctgagcgg tacaatggcc gtggctacgc ctgagaacga tcattttttt gtggatggaa
      301 ttcccgtaac gccttaccag gataacgacc tggtgaatga agatccttac cagcttgccc
      361 tggtggagtt acatgataat cagaacaatc ttattgtgca agcgcaaccg gtcatcccgg
      421 tctcaaatga aataaattgt gtcagttcgg gctgccacag cagtgaggct caaatcctga
      481 acatgcacga agatgaaggg ggatttgatc cgaatgatac accgattctt tgcgcggagt
      541 gccattcttc caatgcactt ggaacacctg gaatgccggg acttgaatcg ctttcgcaag
      601 tgatccatga gcaacatggg gaagagacca acgactgcta caaatgccac ccggggccaa
      661 acacacaatg ccatcgcgac atcatgcaca caatgggaat ggtttgccag gactgccacg
      721 gcagcgtgga tgaagtcggg cagtcgattg atgacggcag ggaaccctgg ctggaggaac
      781 cacaatgcgg caacactgac tgtcatggct caaattatgc tgaggaacag gggaagctat
      841 tcaggcaatc gagaggacat ggtggcctgt tctgcagtgc ctgccatgga tcaccgcacg
      901 ccctttatac cagtgaaaat gacagggaca accagcagat tatcgccctt cagggctatg
      961 ctggtatctt gagagagtgc agcgtttgcc atggtgtaac accggcattt cccggtccgc
     1021 acggctatat acctacctct gtcgaccagc ttgaaggcga agttaaagaa agccttttac
     1081 tttccgttcc ccagccgaat ccttttaaag agcgtactgt gatttctttc aatattgaca
     1141 aaacaggaaa aacatacctg gacatatttg accagaatgg aaaacaaatg gtgcgactgg
     1201 tcagcgaaaa tcttggaaaa ggccaatacc agattacttt taatgcgccg ggatttgcac
     1261 cgggggttta tatcctgaac ctgagaaata acggcaggga atcatcgcag aagctggtac
     1321 tgacaagata aaaacaagaa gagaaaggct gaattctgga acatcctcct aaataattac
     1381 aattccctct aatgcggaat tttgtagttt tgcatttcaa taattaaata tgaagaattt
     1441 atttttagca agcgtgatat gcttgctatt ttcttgcagt ccgggccaaa atcccggcca
     1501 ggataatgcc ggcaaggtta atctggccga ttcgctgcgg taccctgatg aaaaacacct
     1561 ggcaaatctc aggcagctca ccttcggggg agataatgcc gaagcctatt tcagtttcga
     1621 tgataaaagg atcgcttttc agtcgaataa cgcggcatgg aacctggagt gcgaccagat
     1681 cttcactttc gagctggcat ccggcgatat gaaagagggc gcaccccaga tgatcagcac
     1741 cggcatgggg cggacgacct gttcgtattt catgcccggg gatacgacca tcctgtttgc
     1801 ctcgacccat gcgggtgatc atgcctgtcc gcatgttccg ccacccaggc ccgatcacaa
     1861 gtatgtctgg cccatctatg acgattacga catctatgtt tccgacctga aaggcaatat
     1921 catcgacacg ctgacccacg aaccgggata tgacgccgaa gcgaccgttt cgcccaaagg
     1981 cgataagatc gtctttactt cccttcgctc cggcgacctg gagctttata ccatgaacct
     2041 cgatggcagt gacgtcaggc aaatcaccga cgagcttggc tacgacggcg gtgcgttctt
     2101 ctcgcaggat ggcagcatgc tggtattcag ggcttcacgg ccaaaaacgg aggaggatat
     2161 ccgtgtttat aaagagcttc tttcagtagg gcaggttatg ccgacgaata tggagatatt
     2221 tgtctgcaat gccgacggtt cgaatctgag gcaggtgaca aaccttggca aagccaactg
     2281 ggccccctat ttccacccct ccatgaagaa agtgatcttc agctccaatc atgcgtccga
     2341 ccggggctac cagttcaatc tttacatgat caacctggat ggaacagaac ttgaaaagat
     2401 aacgtacgac ggcgttttcg acgctttccc gatgttttcc ttcaacggta aaaaactgat
     2461 cttctcctcc aacaggaata acggaggaac aagggcaacg aaccttttta tcgccgactg
     2521 gattgattag gagatttgaa accgcacttt actgaagttc gtaatcgggc ttgatctcac
     2581 ctaattcgcg caaagcccgc agggtctcca tggcttcttc ttcatcgaga aggctgatcg
     2641 ccgtcccgac atgggccagg acataatccc ccacattggc ttcaggtaaa aattccaggc
     2701 aaatatccgt tacaacaccg tcaaaatcga ctttccccat ctttaacggg gtatcggttt
     2761 ctatcgagat cagttttccc gggacagcca gacacatttc agtttcctat taatagaatt
     2821 tctgcaaaag tccataaatt tctgttataa atccacttcc tttaattgta attttacatc
     2881 aatgttctga ttagagccaa aatcgattag agtcatgaaa aagctatcct tcagcctggc
     2941 aggattggtc atctttttat tgatgatcaa cggatgcgac aaagaatcct catcggccgg
     3001 aatatccgga aaaatcaccg gccattcgga ttgcaaattc tccagatcat cgggaatatc
     3061 gtatgaattg gccgacaccc tgagctgcgt atattacctg tacaacgaaa tttcaggtga
     3121 attgaacatt attcatgtta acagcgggtt taactgttgc cccggagaat tgtcatgcga
     3181 aattaccatc agtcaggata ctattcttat ccgtgaatat gaaaaggagc aggcttgtga
     3241 ttgcaactgc ctatacgatc tctatatcac cgtaaagggt gtgtatatgg ggaattatgt
     3301 gatccgtttt aatgagcctt atgcagaagg catggaagaa ctggttttca aaatcgatct
     3361 ggctgaaaac atcagtggtt cctattgtgt ggccaggaaa aactatccgt gggggatttg
     3421 agggatgcat gaactttcca tagcgatgag cattgtcgaa ctggccgggg aagaagccgc
     3481 aaaggccggt gcatccgaaa tatcgaaaat tgaagtggaa ataggctccc tggccgggat
     3541 tgagacagac gcccttctat tctgctggga tgccgcgatc agggatacaa tggcatgccg
     3601 ggccgccctc gtcattcatc ctattgatgc agaggcgcat tgcctggatt gcggacatgt
     3661 ttttccaatt aatgattatt attcaggatg cccttcctgt caagggtttc aataccggat
     3721 aaccaaagga agagagttgc gtatccgatc gctcaccgtt gaataaaact tactctgagt
     3781 cttatcttcc ggacaaaaaa atgatttttg gctttcctgc aggatcctcc aaatcactgt
     3841 taattatata gttatacccc tcccaaaaat atttttcgga tcgaccgggt atttttcatc
     3901 caaaaaccgg ataaacataa aaaaacggtt atgaaaacaa aaagatggaa aaagggaatg
     3961 gtcgtgatga tcattttgct ggcgggatgg catactccgg ccaggccggg gatcatagta
     4021 accggcatac tggatgggac gctcacgggc ggtagcccga aagcggtcga aatctttgtt
     4081 acggggacgg aaaacctgaa caactacgaa gtgtggcggt cgctcaacgg tgcgccgttc
     4141 ggatcgggtt ccgggtccgt tgcttcttta aatggtgtgt ttacaaacac ttttgtttat
     4201 ttggttaaaa cagatcatgt taatgcattt catgatgttt tcgggaatga agggatcttt
     4261 gccaacgtgg tgccgatggg gattatcaac gggaatggca acgacgggtt ccaggtgagg
     4321 aagaaagtcg gctcggtcgt gatcgaccag gtctggcttg aagatgcttc ttattcttac
     4381 gaagacagct actggtatcg gaaaaacggg accgggcccg acggcgggtg gattccatcc
     4441 gcctgggaaa cgcctggaaa tggtgcgctg gacgggttgg accaggatgg actccgggcc
     4501 gcggtccctt tcggtactta tgcaagtgtc tggctgggaa tcaattcggc gtggaacgac
     4561 agcgtgaact ggtcgctggg atatgtcccc acttctcata ccaacaccgt cattcccgat
     4621 tcgttaaccg aatatccgct gatctttgat ccgccgctcg agccggccac gtgccttaac
     4681 ctgtctatag ctgagaatgc caggctgacg gtagaatcct ccgcctgctt taccatttcc
     4741 ggcaacatgg agatcgaaag ccctgatacg acgggtgctt tgcagggatt gatcctgagc
     4801 tccgatccca accccgaaat gagcggatca ctgatcgtga aaggtacggc cgtgggaaaa
     4861 gtcactgtca ggcggtacat cccgaaaaac aacaactggc atctgatcgc gtcgccggcc
     4921 gatacacaat atttccagcc cgaatttgtc cccgtcccgg tcgaccagag tttcgatctg
     4981 tactactgga acgagacagc ggaatttaac tcggggtgga tcaactgcag ggatgaaaac
     5041 ggcggctggt acccgggatt cgaaaccggg tttcatgaag gtaaagggta cctggtcgcc
     5101 tattcacctg caaccgacgg caacgaagtc cgccggtttt atggccgaat caggacaggg
     5161 gattacagtt tcccgctgag ttgttcgggg aatgcctgga accttctcgg caatccgtat
     5221 acctgcgccc tggattgggc ctcggcaggg atcagcaaag aatccatcgc cggaagtgcc
     5281 gtttacctgt gggacccata cctgaactcg ggacagggtg gataccgggc tcataatggc
     5341 acaaccggcg ttccttcggg
//
LOCUS       DAOQXS010000153         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15643_length_5352_cov_3.863508, whole genome shotgun sequence.
ACCESSION   DAOQXS010000153 DAOQXS010000000
VERSION     DAOQXS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15643_length_5352_cov_3.863508"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKM34_05525"
     CDS             <1..474
                     /locus_tag="PKM34_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ83084.1"
                     /translation="FYGQQDLTESLSSKDKKYIRPKEVDLLALLELNETDYIFIYRSV
                     AVQHGLKFLTLPDEINLKVIENASLYRKASVEIKGTSPGTKMMINGEPISYAITMLNE
                     SPNPGVARQFLHFMLDPDKGMKILEESGHNSLIPVPYRNEFNLPGFLTKYAIYQK"
     gene            498..1508
                     /locus_tag="PKM34_05530"
     CDS             498..1508
                     /locus_tag="PKM34_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83085.1"
                     /translation="MMISAMISEPAKSQTQTNDFYDDAETYNLAGITKIMLDGEIGNP
                     GTVEITAFPLRSLIIKEAVLSGDSNRFTGAYRYDGYSLYDILNHVVLKKKNEAEFPPI
                     IDLYVKVENAKGESVVFSWGEIYYPVHRHEVIIATKVARIVPSKTKDLWPLPTDIKIV
                     AGHDLITDRNISNPLKITVLSANQSYPVNKGLSPLFSPEIKVMSDGKLLDQIDPSRDL
                     GMKLTYETIFYGRGRGIHSTMPFQGIPLKEVLKKYFKITDKNLKTGYFVVASVDGYRV
                     VFTFSEIFNRNDQAELLLIEDKGNHDGGAFRLFPAADFFSDRAVKGVSEIYLLNSNFS
                     PR"
     gene            complement(1475..2200)
                     /gene="lptB"
                     /locus_tag="PKM34_05535"
     CDS             complement(1475..2200)
                     /gene="lptB"
                     /locus_tag="PKM34_05535"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ83086.1"
                     /translation="MILRTEKIVKKYRQRTVVNDVSVEVKQGEIVGLLGPNGAGKTTS
                     FYIIVGLIKPFSGKVFLDEDEITEEPMYRRAQRGIGYLAQEASVFRKLSVEDNIKAVL
                     EFTRLTKGEQKDKLESLLEEFGLQTVRKSLGIQLSGGERRRTEIARSLAVSPKFILLD
                     EPFAGVDPIAVEDIQNIVSHLKDKNIGVLITDHNVHETLSITDRSYLLFEGKVLKSGT
                     SEELAEDEQVRKVYLGEKFELRR"
     gene            complement(2232..4706)
                     /locus_tag="PKM34_05540"
     CDS             complement(2232..4706)
                     /locus_tag="PKM34_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNQ83087.1"
                     /translation="MIRYFRYIFLLLWMIAIVPAIVFSQETRITGKITDARTGEPIPF
                     VNVYFPNTTTGATSDAEGSYALITTHPGDSICASAVGYQLQMAPVQKGKIQTIHFVLT
                     ESTTTLHEIQVRPEERWVELLMKLVIKNKEKNNPDRIETYQCQVYNKIRIDINNVDPQ
                     LRERRIFKPIDFVFEHLDTSEIGQKVFLPSLISESVSDYYFRKSPRSTREVIKASRIS
                     GVENQSFTQYLGGLFLGMNVYDNYINVFDKNFVSPVAGFALFTYEYFLEDTVMVDGKT
                     CYQIRFEPKRKQEMTFYGTLWIHDTTFAVKQIDMRVMPDANFNWVNDFYISQSYDQAG
                     PYWTLTRDYRLVDLNPFKGDVIKTYGIFGHRSTIYSDYVFDNPKEDEFYTSAANVMID
                     PDAYRKEDSWWLVNRPDSLTDTEEQIYEMIDSVKNVPAYKYYEKAVLLLGTGYLVTGN
                     FEIGPLYKFVSFNSIEGLRLRAGGRTSNKFSRKLMLEGHAAYGTKDRQWKYAAGMTYM
                     VGKNPRRSVNLSYRYDMEQLGQDPNAFSEDNFFASFFRRSPADKLTMVSELKTSYEHE
                     WFTGFSNAIRFIRREVYSLGDEKFIINKFGKQILDNSLVAGEIQLYTRFAYRERFLYG
                     EFERTSLGTKYPVAELIYSYGIPGFAGSDVEYHRIQFKLKQRFNVANIGWSKYILEAG
                     KTWGRLPYPFLKIHEGNETFFYYTESGNLMNYYEFISDRYASLFYIHHFDGWFLNKIP
                     LMRKLKWREVVHGRAVWGAMTQENLHYSVLPANSGELRKPYFEVGTGIENIFKVGRID
                     AIWRLSHLDKPDAKRFAIFISVNFTF"
     gene            complement(4760..>5352)
                     /locus_tag="PKM34_05545"
     CDS             complement(4760..>5352)
                     /locus_tag="PKM34_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ83088.1"
                     /translation="GNPSSYLARSADFVLNSSVPKEACPNNLAPTSSSTAQLVMGDAL
                     AVALLECRGFTAGDFARLHPGGSLGKQLYLKVSDLSINNEVPRVDADADISRVIMEIS
                     AKRLGATAVISNGTMVGIITDGDLRRMLEKNTDLKEVRAKDIMTIHPRTIPADSLVVE
                     ALALMRNNNITQLPVTENDRYVGVIHLHDILKEGIL"
BASE COUNT         1407 a         1314 c         1235 g         1396 t
ORIGIN      
        1 ttttacggcc aacaagacct tactgagagc ctttcttcaa aagacaaaaa atacatccgc
       61 ccgaaagagg tcgacctgct ggccctgctg gaattgaacg agacagatta tatcttcatt
      121 tacaggtctg tggctgtgca acatggactt aaattcctga cacttcccga tgaaataaac
      181 ctgaaggtga tcgaaaatgc ttccttatac agaaaggcca gcgtggagat caaaggcacc
      241 agccccggca ctaaaatgat gatcaacggg gaaccgattt catacgcgat taccatgctg
      301 aatgaatcgc caaatcccgg agtggccagg caattcctcc attttatgct ggatccggat
      361 aagggaatga aaatcctgga agaaagcggc cacaactccc taatcccggt cccctacagg
      421 aacgaattca atttacccgg ttttcttact aaatatgcta tttatcagaa atgaaaaaga
      481 tgacgattat ttctcttatg atgatttcag caatgatttc agaaccagcc aaaagccaga
      541 ctcagaccaa cgacttttat gatgatgcgg agacctataa ccttgccggg attacgaaaa
      601 taatgcttga cggtgagatt ggtaatccgg gaactgttga gataaccgcc tttccgctga
      661 ggtcgctgat cattaaagag gccgtgctgt cgggcgattc aaaccgcttc acgggcgctt
      721 accggtacga tgggtattcg ttgtatgata ttctcaatca tgtcgttctg aaaaagaaga
      781 acgaagccga atttcccccg atcatcgatc tgtacgtgaa agtcgaaaat gcaaaagggg
      841 aatcggttgt ctttagctgg ggtgagatct actatcccgt tcaccgccat gaggtcatta
      901 ttgcgacgaa agtcgcacgc attgtgccgt ctaagacgaa agacctatgg ccgctgccta
      961 cagatattaa aattgtggcg ggccatgacc tgatcaccga caggaatatt tccaacccgc
     1021 taaagatcac ggttctttct gccaatcaat cctatccggt caataaaggc ctgagtccgc
     1081 tcttttctcc ggaaattaag gtaatgagcg acggcaagct attggatcaa attgacccat
     1141 ccagggattt gggaatgaaa ctgacctacg aaacgatatt ttacggcaga gggagaggga
     1201 tacacagcac gatgcccttt caagggattc cgctgaaaga agttcttaaa aagtatttta
     1261 agattaccga taaaaacctt aagactggtt attttgtggt ggcttctgtt gatggttacc
     1321 gggttgtttt caccttcagc gagatattca accggaacga ccaggctgaa ttgcttttga
     1381 tagaagataa gggcaaccat gacggcgggg cattccggtt gttcccggca gccgatttct
     1441 tttccgacag agctgtgaaa ggggtttctg aaatctatct tctcaattcg aacttctctc
     1501 ccaggtaaac cttccgtact tgttcatctt cggccagttc ttcggatgtg cccgatttca
     1561 ggacttttcc ttcaaaaaga aggtaggaac ggtcggtgat ggagagagtt tcatggacgt
     1621 tgtggtcggt gatgaggacg ccgatgtttt tatctttaag atgggagacg atattctgga
     1681 tgtcttcgac ggcgatagga tcgacgccgg caaagggctc atccagaagg atgaatttcg
     1741 ggctgacagc cagcgaccgg gcgatctccg tccgcctcct ctcccctccc gaaagctgga
     1801 tacccaggct tttgcgaaca gtctgaaggc caaattcctc cagtaatgat tccagtttgt
     1861 ctttctgttc tcctttagtc agcctggtaa attcaagcac cgccttgatg ttatcttcca
     1921 cgctcaattt tctgaaaacg cttgcttcct gcgccaggta accgataccg cgctgcgccc
     1981 gcctgtacat cggctcctcc gttatctcgt cttcatccag aaagacctta ccggagaacg
     2041 gcttgatcag cccgacgatg atatagaacg atgtcgtctt ccccgccccg ttagggccca
     2101 gcaagccgac gatctcaccc tgtttaactt caacggagac atcattgaca acggtgcgct
     2161 gccggtattt tttaacgatt ttttcggtcc gtaggatcat atctggcaaa tttactgatt
     2221 ctttgaacgc atcagaaggt aaaattaacg gaaatgaaga tcgcaaagcg tttggcgtcc
     2281 ggcttatcaa ggtgcgaaag gcgccagata gcatctatcc gcccgacttt aaatatattc
     2341 tcgatcccgg tgccgacttc aaaataaggc ttcctcaact cgccgctgtt tgccggcaga
     2401 acggaataat gcaggttctc ctgcgtcatt gcgccccaga cggcacgacc gtgaaccact
     2461 tcccgccatt tcagcttccg catgagggga attttattta aaaaccaacc gtcaaaatga
     2521 tgtatataaa ataacgaagc ataacggtcg ctgataaact cgtaataatt catcaggttt
     2581 cccgattcgg tataataaaa gaaggtctcg ttgccttcat ggatcttcag gaacgggtaa
     2641 ggtagccggc cccaggtttt cccggcctca agaatatatt tcgaccaccc gatgttcgcc
     2701 acgttgaacc tttgcttcaa tttaaactga atccggtgat attccacatc acttccggca
     2761 aaacccggaa tgccataact atagatcaac tcggctactg gatatttcgt tccgagagag
     2821 gtccgttcaa attcaccgta cagaaaacgc tcgcgatagg caaaacgtgt atataactgt
     2881 atctcgcctg caaccagaga attatccaga atctgttttc caaatttatt aatgatgaat
     2941 ttttcatctc ccagagaata tacttcacga cggataaagc ggatggcatt ggaaaatccg
     3001 gtaaaccatt catgctcata gctggtcttc agttcactca ccatggtcag cttatcggca
     3061 ggacttcgcc tgaagaacga cgcgaagaaa ttatcctcgc tgaaggcatt cggatcctgg
     3121 cccaattgct ccatatcata gcgataagaa agattgacgc tgcgccgggg atttttaccg
     3181 accatatagg tcatcccggc agcgtatttc cattgccggt cttttgtgcc atatgctgca
     3241 tgaccttcga gcatcagctt gcggctgaat ttattgctgg ttctgccacc cgcccttaac
     3301 cttaatcctt ctatggaatt aaagctgacg aacttgtaca aaggcccgat ctcgaaatta
     3361 cccgtaacga ggtatccggt acccagcagc aaaacagctt tctcataata tttatacgca
     3421 gggacatttt ttaccgaatc gatcatttca tagatctgct cttcggtgtc ggtcaggctg
     3481 tcaggacggt taacaagcca ccaggaatcc tctttccggt aggcatccgg gtcgatcatg
     3541 acattggcgg cagaagtata aaattcatcc tctttcggat tgtcaaagac ataatcggaa
     3601 taaatggtcg accggtggcc gaaaattcca taggttttga tcacatcgcc cttgaacggg
     3661 ttcaggtcga cgagcctgta atcgcgtgtc agggtccagt aaggtcctgc ctgatcatac
     3721 gactgggaga tataaaaatc gttgacccag ttgaaattgg cgtcaggcat gacccgcatg
     3781 tcgatctgtt taacggcaaa tgtcgtatca tggatccaca gggttccgta aaatgtcatc
     3841 tcctgttttc gcttcggttc aaaccggatc tggtagcaag tttttccatc aaccataacg
     3901 gtatcttcca ggaaatattc ataagtaaac aatgcaaaac cagcaaccgg gctgacgaaa
     3961 ttcttatcaa acacattgat ataattgtca tatacgttca ttcccaggaa tagcccgccc
     4021 aggtactggg taaagctctg gttctccaca ccggaaatcc ggcttgcttt gataacctca
     4081 cgggttgacc ggggggattt cctgaaataa taatcggaga ccgactctga gatcagcgaa
     4141 ggcaggaaaa ctttctgacc tatttcactg gtatccaagt gttcaaaaac aaaatcaatc
     4201 ggtttgaaaa tcctcctttc cctcaattgc gggtcgacat tatttatatc gatccggatt
     4261 ttattgtata cctggcattg gtaggtttca atccggtcgg gattgttttt ctccttgttt
     4321 ttaatgacca atttcatgag aagttccacc catctttcct cgggcctgac ctgtatctca
     4381 tggagtgttg tggtggactc ggtgaggaca aaatggatcg tctggatttt acctttctga
     4441 acaggtgcca tctgcaactg gtatcctacc gctgaagcgc agatggagtc tccgggatgg
     4501 gtggtgatta atgcataact cccctcagca tcggaagtag ccccggttgt tgtattggga
     4561 aaataaacat tcacaaaagg aatgggctca ccggtgcggg catcggtgat ctttccggtt
     4621 atccgggtct cctgggaaaa cacgatcgcc ggtacaatcg caatcatcca gagaaggaga
     4681 aaaatatatc ggaaataccg gatcatcggg taaaagtaaa ttcatcgatc ctaaaagaac
     4741 aaccggggac caaaatcgtt cacaagatcc cttcttttaa aatatcatgc aggtgaataa
     4801 caccgacata gcggtcattt tcggtcacag gcaactgggt aatattattg tttctcatca
     4861 gcgccagtgc ctcgacgacc aggctgtcgg ccggaatagt cctgggatgg attgtcataa
     4921 tatcttttgc gcggacctct ttcaggtccg tatttttctc cagcatcctt cgcaggtcgc
     4981 catcggtaat gataccgacc attgttccat tgctgattac ggccgtagcg cccaggcgtt
     5041 tggccgagat ctccatgatc acccggctga tatccgcgtc tgcatcaacc cgcggcacct
     5101 cgttatttat ggagagatcc gacactttca ggtacagttg tttgcccagc gaaccgcccg
     5161 ggtgcagccg ggcaaaatcc cctgcggtaa atccgcggca ctccaggagg gctaccgcca
     5221 gggcgtcacc catcacaagc tgtgccgtag aactgctggt cggggcgagg ttattcggac
     5281 aagcctcttt aggcaccgaa gaattgagca cgaaatcggc tgaccttgcc aggtaagacg
     5341 atgggttccc ga
//
LOCUS       DAOQXS010000154         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15698_length_5339_cov_3.658403, whole genome shotgun sequence.
ACCESSION   DAOQXS010000154 DAOQXS010000000
VERSION     DAOQXS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15698_length_5339_cov_3.658403"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..907
                     /gene="lgt"
                     /locus_tag="PKM34_05550"
     CDS             92..907
                     /gene="lgt"
                     /locus_tag="PKM34_05550"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ83089.1"
                     /translation="MDLLSITWSPDPEIFRIGTFAVRWYGVLFALGFVCGYFIILGFF
                     RREKIPVKLLDQLTTYMVIATVIGARLGHCLFYEPGYYLSNPVEILKIWQGGLASHGA
                     AVGIIFALIIFARKNKVSFLWIIDRIAVVTALAGFFIRMGNLMNSEIFGKPTSLPWGF
                     IYERAGEIAQRTQPRHPSQIYEALAYLLIFALLLFVYRKKDGKPKEGILISLFLVLVF
                     GVRFFIEFLKEPQVGFEAQMSLNLGQWLSIPFVLAGIAGLILIRAKSIKTGRL"
     gene            complement(908..>2466)
                     /locus_tag="PKM34_05555"
     CDS             complement(908..>2466)
                     /locus_tag="PKM34_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83090.1"
                     /translation="NTARNKFYLLPLLLGIAGILYHFKKNRNDTWVVFLLFLMTGIAI
                     VVFLNQTPYQPRERDYAYVGSFYAFAIWIGLGVMGLAEFIQKYVRKQEVAVAAATVGL
                     SFLLVPGIMAHQGWDDHDRSGKYAARDFAMNYLRGCDKNSVLVTFGDNDTFPLWYVQE
                     VEGFRTDVRVVNHMLASGHWYVQQLFSKVYDSDPLPFTLRIDQYNNGVNNYVPVYEIP
                     SLANEYTELSELIRFVALDSDKSKIPISSTKKINFFPSRKVKMTVDAKKCVENGIVPP
                     ELADRIVPVIEWEIKQNALYKNDLAVLDFLASSNWERAFYTANPSSLLDFLDIDQYLH
                     QEGLVYKFMPVKADNYYRGVGGVNGMKTYENFMNCRWGNLNQPNILVDRESYRNSRLP
                     RQNYLRAAETLLINGDKQKAVELLDRCNEFFPDNKINYDMIMIPFAEVYYGAGEPEKG
                     NEIILKLLDYYADDLRYYNTVKPSFVEKYYSDSITRIMRILREIAQVTRTNGQPEISA
                     KATELMESYK"
     assembly_gap    2467..2476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2477..3983)
                     /locus_tag="PKM34_05560"
     CDS             complement(<2477..3983)
                     /locus_tag="PKM34_05560"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNQ83091.1"
                     /translation="MIAHKDFIRWNTLFGWASFLIAFIVYLLTLEPSASWWDCGEYIA
                     TAYKLQVGHPPGAPTFQLIGRFFSLFAFGNTANVAMMINTMSAMVSALTIMFLFWTIT
                     LLARKMVKADAGYTLTQVILLFGSAFVGAMAFTFSDSFWFSASEGEVYAMSSLFTAIT
                     FWAILKWEVVADEKHSLRWLILIAFLIGLSIGIHLLNLLAIPAITFVYYYRKYKPSRK
                     GLLLAGVVSVLILALLMYFFIPGIVKLAGSFELFFVNSIGLPFNSGTIIYFALIIGLI
                     VWGLKYTSKKGKSVLNSIILAFVFLLIGYSSFIILIIRSNANTPIDENNPEDAISLLS
                     YLNREQYGTYPLYYGQYYNAPVVDYKDGTPVYLKDRSKGKYVITDDRKESLPVYDSRF
                     TTLFPRMWSDQKSSHINKYKEYGKIKGTPITVSKPDGTTETLYKPTFVENMRFFFSYQ
                     LGHMYFRYFLWNFAGRQNDIESQGEPQNGNWISGFNFLDNGRLGNQSDLPES"
     gene            4124..4196
                     /locus_tag="PKM34_05565"
     tRNA            4124..4196
                     /locus_tag="PKM34_05565"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4157..4159,aa:Gln,seq:ttg)
     gene            4296..4742
                     /gene="rimP"
                     /locus_tag="PKM34_05570"
     CDS             4296..4742
                     /gene="rimP"
                     /locus_tag="PKM34_05570"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNQ83092.1"
                     /translation="MTIAEVTKIANEYLAGSDKFLVDVLVKPTNRVFVFIDGDQGVTI
                     DDCVKLSRHIESTFDRETEDFELNVSSCGADQPLRFSRQYAKNAGRMLQVKLDDDSIV
                     AGKLVSADESGIVIITNENKKKKVPSETFHLAFNKIKEAKVIISFK"
     gene            4773..>5339
                     /locus_tag="PKM34_05575"
     CDS             4773..>5339
                     /locus_tag="PKM34_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNQ83093.1"
                     /translation="MDHINLVETFSEFKEFKNIDRETMMRILEDVFKHMLEKRFGTSD
                     NFKIIVNIDRGDLEIWRNRVIVEDGSVEDDNTQIALSDAIKIEPDFEVGEEVSEEMKL
                     IDFGRREILSIRQNLVSKIMEYEKDSIYRKYMEKVGDIITGEVYQVWKKEILVLDDEG
                     YELSLPKQEQIPSDFYRKGDTIRAVVARV"
BASE COUNT         1481 a         1216 c         1246 g         1386 t
ORIGIN      
        1 agaacaacag gcagacggga caaataaata attttatcag catcgttcat tatttctatt
       61 tttggcgata aataaattgc ccgagtattc tatggattta ttatcgataa cctggtcgcc
      121 cgaccccgag atattccgga tcggcacctt tgcggttcgc tggtatggcg tgctgttcgc
      181 cctgggattc gtctgcggat attttatcat cctcggtttc tttcgcaggg aaaagatccc
      241 tgtaaaattg ctggatcagt taacaaccta tatggttatt gcaacagtga tcggggccag
      301 gctgggccat tgcctgtttt atgaacccgg ttactacctt tccaacccgg tcgagatact
      361 gaaaatctgg cagggaggac tggccagtca cggggctgca gtgggtatta tattcgccct
      421 catcatcttt gcccgaaaaa acaaagtttc ctttttatgg attatcgacc ggattgccgt
      481 tgttacagcc ctggccggtt ttttcatccg gatgggaaac cttatgaatt cggagatttt
      541 cggtaagccg acctctcttc cctggggttt tatttatgag cgcgccggag aaattgccca
      601 acggacccag ccccggcacc ccagccagat ttatgaagcg ctggcatacc ttctgatttt
      661 cgccctcctc ctcttcgtat acagaaaaaa ggatggtaaa ccgaaagagg ggatactgat
      721 cagccttttc cttgttcttg tattcggagt ccggtttttc attgaattcc tgaaggaacc
      781 acaggtagga tttgaggctc aaatgagcct gaacctgggc caatggctca gtataccttt
      841 tgtacttgcg ggaatagccg gtctgattct tatccgggca aaaagtataa agacgggccg
      901 cctttaacta tttgtaggat tccatcaatt cggttgcctt tgctgatatt tcaggctgac
      961 cgttggtcct ggtcacctgt gcgatttcgc gcaatatcct catgatcctg gttatggagt
     1021 cagagtaata tttttccacg aatgatggtt tcaccgtatt gtaataccgg aggtcatccg
     1081 cataataatc aagcagcttt aggataatct cgttgccttt ttccggttca cccgccccat
     1141 aatagacttc ggcaaacggg atcatgatca tatcgtaatt gattttattg tcagggaaaa
     1201 attcattgca tcggtcaagc aactcaacag ctttttgttt gtcaccatta atgaggagtg
     1261 tttctgcagc ccgcaggtag ttctgcctcg gaagccggct gttccggtaa ctttcccggt
     1321 caaccaggat attgggttgg ttgaggttac cccaacggca attcatgaag ttctcataag
     1381 tcttcattcc gttaactcct cccacaccgc ggtaatagtt atctgctttt accggcatga
     1441 atttgtagac cagcccctcc tggtgaaggt attggtcaat gtcaagaaaa tccagcaggc
     1501 tcgacggatt cgctgtatag aacgcccttt cccagttgct tgatgccagg aaatcgagga
     1561 ccgcaaggtc gtttttataa agtgcatttt gctttatctc ccattcaatg accggaacga
     1621 tccggtcggc cagttcaggt ggcacaatcc cgttctccac gcatttttta gcgtcaaccg
     1681 tcattttcac cttccttgac gggaagaaat tgatcttttt ggttgatgag atcggtattt
     1741 tactcttgtc gctatccaac gccacaaacc ggatcaattc cgataactcg gtatattcgt
     1801 tggccaggct gggaatctca tataccggca cataattatt taccccgttg ttatactggt
     1861 ctattctcag ggtaaaaggc agtggatccg aatcatagac ctttgagaac aactgctgaa
     1921 cataccagtg tcccgaagca agcatatgat tcactaccct gacatcagta cggaaccctt
     1981 caacctcctg gacataccat aaaggaaatg tatcgttgtc gccgaacgtc accaacacgg
     2041 aatttttatc gcaaccccgc aggtaattca tggcaaaatc cctggcggcg tattttccgg
     2101 aacggtcgtg gtcatcccag ccctggtgtg ccattatgcc cggtacgagc aggaaagaaa
     2161 ggccgactgt ggctgcggct acggctactt cttgttttct gacatatttc tgtataaatt
     2221 ccgccagtcc catcaccccc agccctatcc agatcgcgaa tgcataaaac gaaccaacat
     2281 atgcataatc cctttcacgg ggttggtacg gggtctggtt aaggaatacc acgatggcaa
     2341 taccggtcat caggaacaat aaaaacacca cccaggtatc attcctgttc ttcttaaagt
     2401 gataaagtat accagcgata cccagtaaaa gcggcaggag gtaaaacttg tttctagccg
     2461 tattctnnnn nnnnnntact ttccggcaga tccgactgat tcccgagccg gccattatcc
     2521 aggaaattga atcccgaaat ccagttcccg ttttgcggtt ctccctggct ttcgatatca
     2581 ttctgcctac cggcaaaatt ccacaagaaa tagcggaaat acatatgtcc cagttgatag
     2641 ctgaagaaaa accgcatatt ttccacgaat gtcggtttat acaaggtttc agtcgtaccg
     2701 tccggtttac tgacggtgat cggggtccct tttatttttc cgtattcctt gtatttgttg
     2761 atatgggagc tcttttggtc gctccacatc ctgggaaaaa gggtggtgaa ccgggaatca
     2821 taaaccggca aagattcctt ccggtcatcc gtaatcacat attttccttt gcttctgtct
     2881 tttaaataaa cgggcgtacc atccttgtaa tcaaccactg gagcattgta atattgcccg
     2941 taataaagcg ggtaggttcc gtattgttcg cggttgaggt agctcagcaa cgaaatggca
     3001 tcttcgggat tattttcatc aataggggtg ttggcatttg agcggataat cagaatgata
     3061 aatgatgaat aaccgatcag aaggaaaaca aaagcgagta tgatagaatt taaaacactt
     3121 ttgccttttt tcgaagtata cttcagcccc cagacaatca aaccgataat gagggcaaag
     3181 tagataatgg ttcccgagtt gaacggaagg ccgatggagt tgacaaaaaa cagctcgaaa
     3241 gagccggcca gtttcacaat tccggggatg aagaaataca taagcagggc aaggatcaga
     3301 acggatacca ctccggctaa cagcaagcct ttacggctgg gtttgtattt acggtaataa
     3361 taaacgaatg tgatggcagg gatagccagc agattcagga ggtggattcc aatggaaagc
     3421 ccgatcagga aagctatcag gatgagccag cggagggaat gcttttcatc ggcgaccact
     3481 tcccatttca ggatcgccca gaaagtgatg gcggtaaaca aggaagacat ggcatagact
     3541 tcaccttccg aagcggagaa ccagaaagaa tcggaaaaag taaaagccat agcgccgaca
     3601 aacgcactgc cgaaaagcag tatgacctgt gtcagggtat atccggcatc ggctttcacc
     3661 attttccggg ccagcagcgt gatggtccag aaaaggaaca tgatcgtcaa tgcgctgacc
     3721 atggccgaca tagtattgat catcatggcc acattggcgg tatttccaaa tgcaaacagg
     3781 gagaaaaatc ttccgataag ctggaacgtc ggagcccctg gcggatgacc cacctgcaat
     3841 ttgtaggcag tggcgatgta ttccccgcaa tcccaccagc tggcggaagg ctccagggtc
     3901 aacagataaa cgatgaaggc gatcaggaag gaagcccatc cgaaaagggt attccagcgg
     3961 ataaaatctt tgtgtgctat catgctcctt tatttggagt tgcgaaaata gaaagaaaaa
     4021 gcgaatcttt catcccgaat atgactgagc aaatatttct gaagcaaacc gaaatattaa
     4081 ataaataatt tctatctttg cgcgcccaaa acaggggaca gtttgtccga tggtgtaact
     4141 ggcaacacgt ctgattttgg ttcagaagac tccaggttcg agtcctggtc ggacaacagg
     4201 attaatgaaa tagttaatct ttcgggaggg gactgcggtc cccttcttta ttgcatcaaa
     4261 aaatgaaaaa tgaaaaatga aaaataacct gtcacatgac cattgctgaa gttacgaaaa
     4321 tagcgaatga ataccttgcc ggcagcgaca aattcctggt cgacgtactg gtaaagccta
     4381 ccaacagggt atttgtgttc atcgatgggg atcagggggt tactatcgac gattgcgtaa
     4441 aactgagccg gcacatcgaa tcgacatttg accgggaaac tgaagatttt gagttgaatg
     4501 tctcttcctg cggtgccgac cagccgctgc gtttttccag gcaatacgca aagaacgcgg
     4561 gcaggatgct ccaggtcaaa ctggatgacg actccattgt cgccggcaaa cttgtatcgg
     4621 ctgatgaatc cggtattgtc atcatcacca atgaaaataa aaaaaagaag gttccgtctg
     4681 aaacctttca tctcgctttc aataaaataa aagaagcaaa ggtcattata tcattcaaat
     4741 aattgtttaa tccaagctca aacaataaag ccatggacca tataaatctt gttgaaacct
     4801 tttctgaatt taaagaattt aaaaacatcg accgtgaaac gatgatgcgt atcctggaag
     4861 atgttttcaa acatatgctg gaaaaaaggt tcggcacttc cgacaatttc aagattatcg
     4921 tcaatatcga ccggggtgac ctggaaatct ggcggaaccg ggtaattgtg gaagacggat
     4981 cggtagaaga tgacaatact cagattgcac tttccgacgc gatcaagatc gaacccgact
     5041 ttgaagtggg cgaggaagtg tcggaagaga tgaaactgat cgacttcggg cgaagggaaa
     5101 ttctctccat ccggcagaac ctggtgtcga aaatcatgga atatgagaaa gacagcatct
     5161 accggaaata catggaaaaa gtcggcgaca tcatcaccgg cgaagtttac caggtctgga
     5221 aaaaagagat cctggtcctg gacgatgaag ggtatgaact gagcctgccg aaacaggaac
     5281 agataccctc cgacttttac cggaaaggcg atacgatccg ggcagttgtt gcacgggtt
//
LOCUS       DAOQXS010000155         5334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15717_length_5334_cov_3.684220, whole genome shotgun sequence.
ACCESSION   DAOQXS010000155 DAOQXS010000000
VERSION     DAOQXS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15717_length_5334_cov_3.684220"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKM34_05580"
     CDS             <1..637
                     /locus_tag="PKM34_05580"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNQ83094.1"
                     /translation="WNTNVPYDDLIHVFASNTKDYIRFYTNYSDIQGGENSIVLQGYY
                     AKRYWQEGNIIQYPEFLDPENGDYSLNWNSPCIEAGRPDTTGLHLPETDLNGNPRIVN
                     DRIDMGAFEFQYPLAVSQPITARQENGIIISHSESHIKVITSSSLQDKTLAIRLISSD
                     GKTIFSTTKPEGETQTSITTNEIPAGIYLVTVSDHDKLLKSEKIVVSQSLR"
     gene            729..1229
                     /locus_tag="PKM34_05585"
     CDS             729..1229
                     /locus_tag="PKM34_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNQ83095.1"
                     /translation="MLKLVFLILMTVMSLPVAYSQDNGKDKIRTMNFDQLEPLLHRQN
                     DTVYLVNFWATWCAPCRKEMPAIQSVAAKYADQPFRTLLVSLDIPGQIEKSLLPYIEA
                     GNITQEVILLDDPDQNRWIDLVDPSWSGELPFTLIYGNDFREGHSEVFTFEILDSIIH
                     SKIKRP"
     gene            1226..1837
                     /locus_tag="PKM34_05590"
     CDS             1226..1837
                     /locus_tag="PKM34_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ83096.1"
                     /translation="MKLFALSLILFALTIPTLFAQGYQIGDKASSFSLRNVDGKQVSP
                     GDYPDAKGFTVIFTCNHCPYAIAYQDRIIELDKKYRELGYPVIAINPNDPELAPEDSF
                     DKMAVRAREKNFTFPYLFDETQEVYRKFGAKRTPHVYVLQKESADLVVRYIGAIDDNY
                     QDALKVTAHYLADALDALIAGNKPDPDFTKAIGCSIKDKQAVK"
     gene            1957..2442
                     /locus_tag="PKM34_05595"
     CDS             1957..2442
                     /locus_tag="PKM34_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ83097.1"
                     /translation="MTVNEYNQCVDQHSDGVYRFILKNIRDEEAAMDIVQDAFEKMWM
                     KFREVKASSAKSYLFTTAYNRMIDYTRKEKRMERMVEGYDYGQMDPGHYNDVKQVLDE
                     ALERLPEIQKSVILLRDYEGYNYEEIGQITGLNESQVKVYIFRGRMALKNYLVSIENL
                     V"
     gene            2443..3513
                     /locus_tag="PKM34_05600"
     CDS             2443..3513
                     /locus_tag="PKM34_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83098.1"
                     /translation="MKITRDNYEQYFLDHAEGRLSSAMESELADFLEANPDLRYVLDS
                     YDASPLPPDDLTNENLRRRLKKRVTPTKHIGETNIDEWLVRDLEGLLAADEEAELESF
                     ISNNPAFEYDRKLFGMTIQNPDHSIKYTGKGKLKKRGVVLSMTRIGWIATAAAAVLLL
                     MTVSRFWPEPLPEELVPDETPSALAETPSRIKETPSQIKETPSALPETPSQIKETPSH
                     RARMESRIIEMESQFLTPLLTRNIAIDQNPVLLQASLDYHPLPENIMPETEEKGLLAS
                     VVSNLFGKARDAFRENPTFERLSETDLNLWAIAQAGVNGYNRISDRDLALYVHKDQDG
                     NVLSYALVEQDKLLLSKHLDKE"
     gene            3577..5127
                     /locus_tag="PKM34_05605"
     CDS             3577..5127
                     /locus_tag="PKM34_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNQ83099.1"
                     /translation="MKTMHNLRGSFLGVLISILFMITTTAFSQKEIVRETRDLPAFNQ
                     IDAGGAFQFILVPGTPQKVGVEARQKDIGKIATEVKDNKLSVTGKGISNNPVLNVYIT
                     MPSLTRLEAHGAADVESEGTFKEEMMSLEVSGAASVDLSLDVKTLSSDVSGAADLELT
                     GKAERHSTVVSGAGTLNAFDLKTVTTDAEVSGAGTAKVSAGELTGESSGAGKIYSDDG
                     NWAGNDTKSIVEVDDDGDTTYIKVPGTSVIISEGDDSVKVKVGNRVIIIDDDGNVKTR
                     HHKWPKFNGHWAGVDIGLNGFVTPDFNMNLPREYEYLDLRMEKSIAVNLNFFEQNIPL
                     SKNQKWGIVTGLGLGFNDYRFLRPTRLSVDSSFLVGYIDKGIEIRKSKLSAMYLTLPV
                     LFEFQTAPVYHNNGFHINVGVVVGARLSSHTKKYYEDLNAEFDVTQYNPATGMYETVF
                     TAISPEQHKTRVYDDYFLQPFKFDATARIGWNFLNFWASYSLNSMFREGKGPELYPWG
                     AGITLVCF"
BASE COUNT         1479 a         1302 c         1263 g         1290 t
ORIGIN      
        1 ctggaacacc aatgtccctt acgacgattt gattcatgtg tttgccagta acacaaaaga
       61 ttacatccgc ttctacacga attattccga tatccagggc ggggaaaact cgattgtact
      121 gcaaggatat tatgccaaac gttactggca ggagggcaac atcattcagt acccggagtt
      181 tttggatcct gaaaacggtg attattccct caactggaac tctccctgca tcgaagccgg
      241 caggcccgat actaccggtt tgcatctccc cgaaaccgat cttaacggca atccccgcat
      301 cgtgaacgac cggatcgaca tgggcgcctt tgaattccaa tacccgttag ctgtcagcca
      361 acccatcaca gcccggcagg aaaacgggat catcataagt cattcagaat cacacataaa
      421 agtaattaca tcatcatctc ttcaagataa aaccctagct atccggctca tttcatccga
      481 tggcaaaacg atcttttcga caaccaaacc ggagggtgag acccagacct ccattacaac
      541 caacgaaatc ccggccggta tctacctggt cacggtatca gatcacgaca aattgctgaa
      601 atcagaaaag atcgtggttt ctcaatcgct gcgttaattc ttacatataa ctgcagaact
      661 tcggctgaat tttaataagg atgaagaacc ttttccatct ttcattgtta taatccaaaa
      721 cgattttaat gctcaaactt gtgttcctga tcctcatgac ggtcatgtcg ttgccggtgg
      781 catactccca ggataacggt aaggataaaa taaggacgat gaattttgat cagctggagc
      841 ccttgctgca caggcaaaac gacaccgttt acctggttaa cttctgggct acctggtgtg
      901 cgccatgccg caaggaaatg cctgccattc aatctgttgc ggcaaagtac gccgatcaac
      961 ccttccggac tttgctggta agccttgata tccccggaca gattgagaaa agcctgttgc
     1021 catatattga agccggaaat atcacccagg aggttatact tctcgacgat ccggaccaga
     1081 accgatggat cgacctggtt gatccctcct ggtcgggaga actgcctttt actttgattt
     1141 acggaaatga tttccgggaa ggccattctg aagtttttac attcgaaatc ctcgattcaa
     1201 ttattcactc taaaattaaa cgaccatgaa actattcgca ttatctctca ttctatttgc
     1261 attgacaatc ccgacactgt ttgcccaggg ttaccagatc ggcgacaaag cctcatcatt
     1321 cagtctcagg aacgttgacg gaaagcaagt ctcacccggt gattatcctg atgctaaagg
     1381 ctttacggtg atattcactt gcaaccactg cccttatgcc atagcctacc aggaccggat
     1441 catcgaactg gataaaaagt accgggaact gggatatcct gtcattgcca tcaatcccaa
     1501 cgatcctgaa ctggccccgg aagactcttt tgataaaatg gcagtgaggg ccagggagaa
     1561 gaattttacg tttccttacc tttttgacga aacccaggag gtgtacagga aattcggagc
     1621 caaacgtaca ccgcatgttt atgtccttca aaaagagagc gctgatcttg ttgtccggta
     1681 tatcggtgca atcgacgaca actaccagga tgccttaaaa gtcacggcac attacctggc
     1741 cgacgcgctc gacgccctga tcgccggcaa caagcccgat ccggatttta ccaaggccat
     1801 cggttgctcg attaaagaca aacaggctgt aaaataaggt attgcgtcaa tccggcacaa
     1861 tcccatccgg aaaagttatt ccagattcta ccctgttgta acttttcata cccttaaccg
     1921 tcttatgcat aactttgtgg aattcaaagc tgcagcatga ctgtaaacga gtacaatcaa
     1981 tgtgtggatc agcactccga cggggtgtac cgtttcatcc tgaagaatat cagggatgaa
     2041 gaggcagcga tggatattgt tcaggatgca tttgagaaga tgtggatgaa attcagggaa
     2101 gtgaaagcat ccagcgccaa atcctatctt ttcacaacgg cttacaaccg gatgatcgat
     2161 tatacacgca aggagaaacg gatggagcgg atggtcgagg gttacgatta cggacaaatg
     2221 gatcccggcc attacaatga tgtgaaacag gtgctggatg aagcgctgga gcggttgccg
     2281 gaaatccaga aatcggtcat cctgctgcgg gattatgaag gttacaatta tgaagaaata
     2341 gggcagatca caggattgaa cgaatcgcag gtaaaggttt atattttccg gggccgtatg
     2401 gctttgaaga attatctggt cagcatagaa aatttggttt agatgaaaat cacgagggac
     2461 aattacgagc aatattttct cgaccatgcg gagggccggt tatcctcagc catggagagc
     2521 gagcttgcgg attttcttga ggccaatccc gatctgcgct atgtgctcga cagctatgat
     2581 gcttcacccc tgccccccga cgatctgacc aatgaaaatc tgcgcaggag gttaaaaaaa
     2641 agggttactc ccacgaagca tatcggggaa actaatatcg acgaatggct ggtccgggac
     2701 ctggaagggt tgctggcagc cgacgaagaa gccgagctcg aatcttttat ttcaaataat
     2761 cctgcgtttg aatacgaccg gaagctgttt gggatgacca ttcaaaatcc ggatcattcc
     2821 attaaatata caggtaaggg aaagttaaaa aagaggggtg tggtgttgtc gatgacaagg
     2881 ataggctgga tagccacagc ggctgcagcc gtattgttat taatgaccgt atcccgtttt
     2941 tggccggaac ctttgccgga ggaactggtt cccgatgaga ctccatctgc cctggccgag
     3001 actccatctc ggatcaagga gactccatct caaatcaagg agactccatc tgcccttcca
     3061 gagactccat ctcaaatcaa ggagactcca tctcaccgtg cccggatgga gtcgcggata
     3121 attgagatgg agtctcaatt cctgaccccg ctcctaaccc gcaatattgc tattgatcaa
     3181 aaccccgtat tgctccaggc ttcgcttgat tatcacccct tacctgaaaa tataatgccg
     3241 gaaactgaag aaaaaggact cctggccagc gtggtctcca atctcttcgg caaggcccgc
     3301 gatgccttcc gcgaaaatcc taccttcgaa aggctttcgg aaactgatct caatctgtgg
     3361 gcgatcgcgc aggccggtgt gaatggatac aacagaatct ccgaccgcga cctggcgctg
     3421 tatgtccata aagatcagga tggaaatgtg ttgtcctatg cccttgttga gcaggataaa
     3481 ctcttgttat ccaaacatct ggataaagaa taatattgcc tgtaacattt tcattaccct
     3541 gcccgtcaca ggaaaaaaac taataatctt tattatatga aaacgatgca caatttaaga
     3601 ggatcatttt tgggagtgct tatcagcatt ttgttcatga tcacaacaac cgccttcagc
     3661 cagaaagaga tcgtaaggga aacccgcgat cttccggctt ttaatcagat tgacgccggg
     3721 ggcgcctttc aatttatcct ggttccggga actccacaaa aagtaggggt cgaagcccgg
     3781 caaaaggata tcgggaaaat cgctaccgaa gtgaaagata ataaactaag cgtcacgggc
     3841 aaagggatca gcaataaccc cgtacttaat gtttacatta ccatgccttc cctgacccgg
     3901 ctggaagctc atggagctgc ggatgttgag agtgaaggga cattcaaaga agagatgatg
     3961 tcgctggaag tgtccggagc ggcatccgtc gatttgtcgc tcgatgtcaa aaccctcagc
     4021 tctgatgtat ctggagcggc agaccttgaa ctgacgggca aagcggaacg gcatagcacg
     4081 gtggtcagcg gagccggcac actcaatgcc tttgacctga aaacagtcac taccgatgct
     4141 gaagtcagcg gggccggaac agcgaaagtt tccgccggcg aactaaccgg ggaatccagc
     4201 ggcgcgggca aaatctattc ggatgacgga aactgggccg gcaatgacac caagtccatc
     4261 gttgaagtgg atgatgacgg cgacactact tatatcaaag taccgggaac cagcgtgatc
     4321 atttcggaag gcgatgattc cgtcaaagtg aaggtcggta accgggtgat cattattgat
     4381 gatgatggca atgtcaaaac aagacaccac aaatggccca aattcaacgg acattgggcc
     4441 ggcgtggata ttggcctgaa tggctttgtg actcccgact tcaacatgaa cctaccgcgc
     4501 gagtacgaat acctcgacct gcgcatggaa aaatccatcg ccgtcaatct aaacttcttc
     4561 gaacaaaata tccctttgtc taaaaaccag aagtggggca ttgtcaccgg ccttggcctg
     4621 ggattcaatg actaccggtt cctgcggcca acccgcttga gcgtcgattc atcattcctg
     4681 gtcggctata tcgacaaagg cattgagatt cggaaatcaa aactttcagc tatgtacctg
     4741 acgttgcctg tcctgttcga attccagacc gctcccgttt atcataacaa cgggtttcat
     4801 atcaacgtcg gagtcgtggt cggagccagg ctttcatcgc ataccaaaaa atactatgag
     4861 gatttgaatg ccgaattcga cgtaacacaa tacaatcccg caaccgggat gtatgaaacc
     4921 gttttcacgg ctatttcacc ggaacagcac aaaaccagag tttacgacga ttatttcctg
     4981 caacctttca aattcgatgc aactgcccgg atcggatgga atttcctgaa cttctgggcc
     5041 agttattcgc ttaattcgat gttcagggaa gggaaaggac cggaactgta tccctgggga
     5101 gccgggatca cgctggtgtg tttctaggtt ccaggttcca ggtttcgggt tccaggttcc
     5161 aggttccaaa gttaaaattc ctctgaactc cataagagtg ccagtatggt agggatttga
     5221 ggccgtcggt tctggatggg ttttccttcc gacgactaaa gccgtcggtt ccaattgcaa
     5281 ctccttggaa cagacggctt tagtcgtcgg aaggaaaacc catccagaac cgac
//
LOCUS       DAOQXS010000156         5329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15737_length_5329_cov_3.622677, whole genome shotgun sequence.
ACCESSION   DAOQXS010000156 DAOQXS010000000
VERSION     DAOQXS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15737_length_5329_cov_3.622677"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKM34_05610"
     CDS             <1..393
                     /locus_tag="PKM34_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ83100.1"
                     /translation="TILQAIPAGFVKTIDGIANYLKQLKLLFSPEIKAYESVGGFISI
                     GSIFPATWDWQAFWRLTAFLSIMLAILNLLPIPALDGGHVMFLVYEIVSGRKPSDKFM
                     EYAQIVGMVLLFGLLIFANLNDVLRLFK"
     gene            complement(524..1420)
                     /locus_tag="PKM34_05615"
     CDS             complement(524..1420)
                     /locus_tag="PKM34_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNQ83101.1"
                     /translation="MSFVTKEKLFSKKWFIAYSLIALGAFVMAAGFVLFITPYKIVPG
                     GVYGISIILHHLFDFPVGLTAICLDIPITIIGTRILGPRFGVKTVVGFTLTAVFVDTL
                     TYFWGDLPLVQDNGLLSSIYGGVLVGIGLGLIFKARATSGGSDVIAMIISKFNKMPPG
                     QMIILVDSIVVIAGLFSFMDWVIPLYSLLTIFITGRVMDVILQGISYDKTLFIISDKH
                     ELIRDKIINDINRGGTYIQGHGMFGGQEKTIIFTNVNRREMAILQEYIHHIDPMAFMT
                     VINANEVLGEGFRSLKEKISSD"
     gene            1584..2567
                     /locus_tag="PKM34_05620"
     CDS             1584..2567
                     /locus_tag="PKM34_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNQ83102.1"
                     /translation="MNFSKIAKLNVLPVLVFLWLAFPAHSQQEPNFSQYMFYGLNLNP
                     AFAGTEPAVNITAANRMQWTGFGKENGDQVAPRTYFIAADLPVRVLKGGIGAVIMNDA
                     IGHENTISVKIGYANQRNMGFGKLGIGAQLEFNNRSIDFSKLDPAGEDPLLNQLSSEE
                     SEMLIDFSLGLLYRVPDNYYIGVSGLHLIQTKGKPLVESSEGSLRMKMDRTFMITGGY
                     EVNFPRNPDFQFVPSVIIESNIAKTRLDINAMLRYKDAVWGGVGYRLGESVIILLGVQ
                     YKDFRIGYSYDINANRLALPIFGGTHEIMVNYRFKLEIDRGRKSYKNTRFL"
     gene            2672..4051
                     /locus_tag="PKM34_05625"
     CDS             2672..4051
                     /locus_tag="PKM34_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ83103.1"
                     /translation="MKRLPAFLLILALSGILISCGDTGNGELVGVSRKTRPFFQADPY
                     GMVFIPQGSFTMGVGDQDKSYGFIYEPKTVSVASFYMDETEITNDEYRQFVNWVKDSI
                     ALRKLGDIRPDEYLIQENPKTGEIYDPPFLNWSAEIDWESEEQDIRDALEEMYVPEHE
                     RYFRKKELDTRQLFYEYYWIDYVAAARKDWSEEANTEDGAFANRPQGRRDRSVYIRKE
                     RLNVYPDTLSWIYDYTYSFNDPQTKSYFWHPAYNFYPVVGINWKQARAFCIWRTELLN
                     SKMDPRKGEPGIVDFRLPTETEWEWAARGGYNNNPYPWGGPYTRNERGCFLANFKPLR
                     GNYIDDGGLRTVIVAHYPPNDFGLYDIAGNVSEWTNTAFDPSTYNFTWDMNPNYTYNA
                     KEDDPPALKRKVVRGGSWKDIAYFMQVSTRDYEYQDTAKSYLGFRCILPYLGRNKDDN
                     PARASRVYN"
     gene            4107..5129
                     /gene="gldL"
                     /locus_tag="PKM34_05630"
     CDS             4107..5129
                     /gene="gldL"
                     /locus_tag="PKM34_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNQ83104.1"
                     /translation="MGLNTFVRRRGFRNFMSKLYGWGASVVILGALFKINHYPFANEM
                     LIVGLGTEAIIFFFSAFEPPYVEPDWSLVYPELGGLYHGDETAESVKKKPTQQLDDLL
                     KQSNIDQKLMDRLGDGLRKLSDNTSKLSEITDAAGVTNEYLTKVKGAAKSVENLSKSY
                     DVTAETLQKDVNASAGYSNSIKAAGEHAAQLSNVYKEASETLKGDINVTKEFTNSMKT
                     AMQSANSLAEQYTKSAEALSLSAKQLDFATVDGKSYTEKLHQISEKLAALNSVYELQL
                     QSNKDQMQSTAQVRDSMAQLLKTMKESADTMATYKDQMNLLTQRISSLNEVYGGMLTA
                     MSGKAK"
BASE COUNT         1515 a         1235 c         1243 g         1336 t
ORIGIN      
        1 actattcttc aggctattcc tgccggtttt gtgaaaacca tcgacggaat agctaattac
       61 ctgaaacaat taaagttgtt gttctcgccg gaaatcaaag cgtatgaatc tgtcggcggg
      121 tttatctcca tcggaagcat tttcccggct acatgggatt ggcaggcttt ctggcggctt
      181 accgcattcc tgtccatcat gctggccatc ctcaaccttt tgcccattcc cgccctcgac
      241 ggcggccacg tcatgttcct ggtatacgaa atcgtcagcg gacgaaaacc cagcgacaaa
      301 ttcatggaat atgcccagat tgtcggtatg gtattacttt tcggattgct gatattcgcc
      361 aacctgaacg acgtgctccg gctctttaaa tagtgaatgg tgaagggtga agggtgaagg
      421 gtgaagggtg aacggtgaag ggtgaacggt gaagggtgaa cggtgaacca gaattcaaaa
      481 cccggaacct ggaacccgga acctggaacc cggaatttaa tttctaatcc gaactgatct
      541 tctctttcag cgaccggaat ccctccccga gaacctcatt ggcattgata acggtcataa
      601 aggccatagg atcgatatga tggatgtatt cctggaggat tgccatctcc cggcggttaa
      661 cattggtgaa tattatggtc ttttcctggc ccccgaacat tccgtgaccc tggatgtaag
      721 tccctccccg gttgatatcg ttgatgatct tatcccggat caattcatgc ttgtctgata
      781 tgatgaacag ggttttatca tagctgatcc cttgcagtat gacatccatc accctgcccg
      841 taatgaaaat ggtcagcagc gaatacaggg ggataaccca gtccataaaa gagaaaagac
      901 cggcaatcac gacaatcgaa tcgaccagga tgatcatttg cccgggaggc atcttgttga
      961 attttgagat gatcatggcg atgacgtccg atccgccgga ggtggcccgc gctttaaaaa
     1021 tcaaacccag gccaatcccg accagcactc cgccataaat ggaggaaagc aggccgttgt
     1081 cctgcaccag gggcaggtca ccccagaaat aagtcagtgt atcgacaaag acggctgtca
     1141 gtgtgaaccc gacgaccgtt ttaaccccga atctcggccc caggatccgt gtcccgatga
     1201 ttgtaatcgg aatatcgagg caaatggccg tcaacccgac cgggaaatca aacaggtggt
     1261 gcaggatgat ggagatacca taaacccctc ccggcacaat cttgtaagga gtgataaaca
     1321 aaacaaaccc ggcagccatg acaaaagccc ccagggcgat cagcgagtag gctatgaacc
     1381 attttttgct gaatagtttt tctttggtaa caaacgacat atcatttaga tttatatccg
     1441 atggccaaaa atagggataa tcattcctcg attttgttac ccggatatca ctttgtaaaa
     1501 tattcgtaaa atatcaccgt ttaagacttc ggtgaaaaat tttcttaatt tagcgtttta
     1561 ttttactaac ccgtttcctg aagatgaatt ttagcaagat tgctaaactg aatgttctgc
     1621 cagtacttgt ttttctgtgg cttgcctttc cggcacacag ccagcaggaa ccgaattttt
     1681 cacaatacat gttttatggc ctgaacctga accccgcatt tgccggaacg gaacccgcag
     1741 tgaatatcac agcagcaaac cggatgcagt ggacgggatt tggcaaagaa aacggggacc
     1801 aggtggcgcc gcgtacctat tttattgcgg ccgatctgcc ggtccgcgtg ttaaaaggag
     1861 gtatcggagc cgttattatg aatgatgcca tcgggcatga aaatacgatt tcggtaaaga
     1921 tcggctatgc caaccagcgg aatatgggat tcggcaaact gggcatcggg gctcagctag
     1981 agtttaacaa ccgcagcatc gacttcagca aactggatcc ggccggcgaa gatccattgc
     2041 tcaatcagct ttcatcagag gaaagtgaaa tgctgatcga cttctccctg ggcttgttgt
     2101 accgggttcc cgataactac tatatcggag tttccgggct tcatcttatc caaacaaaag
     2161 ggaaacccct tgtcgaatct tcggaaggga gcctgagaat gaaaatggac cggacgttca
     2221 tgatcacggg cggatatgag gtgaattttc cccgtaaccc cgactttcaa ttcgtccctt
     2281 cggttatcat cgaatccaat attgcaaaaa cccggctgga tatcaacgcc atgctccgtt
     2341 ataaagacgc tgtctggggg ggagttggct acaggctcgg cgaatcggtc atcatcctgc
     2401 ttggtgtcca gtataaggac ttccggatcg gttattctta cgatatcaat gccaaccgtt
     2461 tagcgctgcc catttttgga ggcactcacg aaatcatggt taattatcgc tttaaactgg
     2521 aaattgacag gggcaggaaa agttataaga ataccagatt cttataatca ttgaaaaaaa
     2581 tagttgtttt gttttaatat tttttatttt tgggcacttt ttaaaataat cctatacgat
     2641 gttcgttaat acttggtcaa aaaaattcgt tatgaaaaga cttccggcat ttttgctgat
     2701 cctcgcatta tccggcattt taatcagttg tggtgatacc ggaaacggag aactggtggg
     2761 tgtcagccga aaaacaagac cattctttca ggccgatcct tatggaatgg tatttatacc
     2821 gcagggtagc ttcacaatgg gtgttggcga ccaggacaaa tcatatggat tcatttatga
     2881 acccaaaacc gtttcggtag cctcgtttta tatggatgaa accgaaatca caaacgacga
     2941 gtaccgtcag tttgtcaatt gggtcaagga ttccatcgcc ctgcgcaaac tgggcgatat
     3001 ccggccggat gaatacctta ttcaggaaaa tcccaagacg ggcgaaatct acgatccgcc
     3061 gtttttaaac tggtcggcag aaatagattg ggaaagcgaa gagcaggata tccgcgatgc
     3121 acttgaagaa atgtatgtcc cggaacatga acgatatttc cgtaaaaaag aactggatac
     3181 ccggcaattg ttttatgagt attactggat agattatgtg gctgccgccc gtaaagactg
     3241 gtcggaagaa gccaacaccg aagatggagc ttttgccaac aggccgcagg gcaggcgcga
     3301 ccgctcggtc tatataagga aagaaaggct gaatgtttat cccgataccc tgagctggat
     3361 ttacgattat acatactctt tcaacgatcc tcagactaaa tcctatttct ggcatccggc
     3421 atacaatttt tacccggtcg ttggtataaa ctggaaacag gccagggcat tctgtatctg
     3481 gcgtaccgag ttgcttaaca gcaaaatgga cccgagaaag ggtgaacccg gtattgtcga
     3541 tttccggctt cccacagaaa cagaatggga atgggcagcc cggggaggct ataacaataa
     3601 tccttacccg tggggaggcc cttatacccg taatgaaagg ggctgtttcc tggctaattt
     3661 caagccgctc agaggaaatt atatcgatga cggaggtttg cgtacagtca ttgttgcaca
     3721 ttaccccccc aatgatttcg gcctgtatga tatagccgga aatgtctcgg aatggacaaa
     3781 tacagcattt gatccgtcga cttataactt cacctgggat atgaatccca attatacata
     3841 caacgccaag gaagatgacc cgcctgccct gaaaagaaaa gttgtacgcg ggggctcgtg
     3901 gaaagatatt gcatacttta tgcaggtatc cacccgtgat tatgaatacc aggatactgc
     3961 caagagctac ctgggattcc gttgtatact tccttacctc ggccgtaata aagatgacaa
     4021 tccggccagg gcttcaaggg tctataacta attcgttcag aaattctcat taaatcaacc
     4081 aataaaaact tatttattct aatatcatgg gtctcaacac atttgtcaga aggcgcggtt
     4141 tcaggaattt catgtccaaa ctttacggct ggggcgcatc agtcgttatt ctcggcgctt
     4201 tgttcaaaat caaccattac ccgtttgcaa atgaaatgct tatcgtgggt ttgggaacag
     4261 aagccatcat tttcttcttc tcagcgttcg aacctccgta tgtcgagccc gactggagtc
     4321 tcgtttaccc ggaactggga ggtttatacc atggcgatga aacagcggaa tcggttaaaa
     4381 agaagcccac acagcaattg gacgatctgc tgaaacaatc gaatatcgac cagaaactaa
     4441 tggaccgact cggcgacgga ctccggaaat tgagcgacaa tacatccaaa ctttccgaaa
     4501 taaccgatgc agccggggta accaatgagt acctcaccaa agttaaaggc gctgccaaat
     4561 cggttgaaaa cctttcaaaa tcttatgatg tcaccgccga aacccttcag aaagatgtga
     4621 atgcttcggc tggatattcc aacagcataa aagctgccgg agaacatgcc gctcagcttt
     4681 ccaatgtata taaggaagct tctgaaactc tcaaaggcga tatcaatgtc accaaagagt
     4741 ttaccaacag catgaaaact gctatgcagt cagccaattc actggccgaa cagtatacca
     4801 aatcggcaga agccctctca ctttccgcta aacaactcga ctttgctacc gtcgacggaa
     4861 agtcctatac ggaaaaactt catcagatat cggaaaaact ggctgccctg aattcagtct
     4921 atgaacttca gctgcagagt aataaggatc agatgcagtc gactgctcag gtccgcgatt
     4981 ccatggcaca gctgctcaaa acaatgaaag aatcagccga tacgatggca acctataagg
     5041 accagatgaa cttattgacg cagcggatct cttcgctcaa cgaagtgtac ggaggtatgc
     5101 tgacagctat gagcgggaaa gccaaatagt caatgcaggc aacagttaac cagattttta
     5161 acaagattaa atccgaaaga tatggctgga tataaagaga caccacggca gaaaatgatc
     5221 gccatgatgt atcttgtgct gactgccctg ctggctctta atgttaccaa ggacatgctc
     5281 gaggctttcc tcgtggtaaa cgaaagtatg gagaatacta tcgaagtct
//
LOCUS       DAOQXS010000157         5318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15781_length_5318_cov_3.422193, whole genome shotgun sequence.
ACCESSION   DAOQXS010000157 DAOQXS010000000
VERSION     DAOQXS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15781_length_5318_cov_3.422193"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..769)
                     /locus_tag="PKM34_05635"
     CDS             complement(77..769)
                     /locus_tag="PKM34_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ83105.1"
                     /translation="MIQLLTGFLAAAVHVVTGPDHLAAVTPLAIENRKKAWNIGLMWG
                     IGHVAGMLLIGLLYLAFKSMINVEKISGYSEFLVGFVLIGIGAWAIVKTLGRFHFHHS
                     HPHYHEKPSPQVHIHRHDHHDETNHTHIHSKSTVQNSITALLIGTLHGFAGISHFLLI
                     LPTLALPSVSESVIYLAGFAAGTISAMIIYAFIMGYIGQKTANFAYSAAFRNLRIIAG
                     ALAIAVGVWWLF"
     gene            complement(784..1398)
                     /locus_tag="PKM34_05640"
     CDS             complement(784..1398)
                     /locus_tag="PKM34_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HNQ83106.1"
                     /translation="MMTTKGQDIHGHSGQSGVIFSGFPVHQEDYARDVKEIMAETIKK
                     YGEEEWRLVALTSEIHGHLGIYAIIGAKMGLYAKEILEAGHDELRITSYAGSKPPVSC
                     LNDGLQVSTGATLGHGLIKIGESESPLPAATFTLHDKSIRLTLKRDIFAGIENQIKAA
                     LKESGGLTPAYWVRIRELGLAVWKGYDRKGLFEVIEGTGTLKIN"
     gene            complement(1478..1870)
                     /gene="nikR"
                     /locus_tag="PKM34_05645"
     CDS             complement(1478..1870)
                     /gene="nikR"
                     /locus_tag="PKM34_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNQ83107.1"
                     /translation="MNIKRFGVSLEDELLQALDEYARQNKFPNRSQAIRHLIRKNLIK
                     RQWEGNQEVAGAIVLVYDHHKRDLQHNSTHIQHDYHDLILSVQHVHLDHHHCLETIAV
                     KGRADRLTDLANKLIGLKGIFHGELVMS"
     gene            1972..2727
                     /gene="truA"
                     /locus_tag="PKM34_05650"
     CDS             1972..2727
                     /gene="truA"
                     /locus_tag="PKM34_05650"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNQ83108.1"
                     /translation="MHHRYFIKLAYNGARYHGWQIQDNAITVQQVITDAVKLMWSRDF
                     RMIGCGRTDTGVSAKQFYAHFDLPDEKSREELSGLAHRLNRYLPEDIVIYDIFPVPPD
                     LHSRYDAVFRTYEYHIHTRKDPFLNPMSWYVYPAPGVDRMNEACLVLKEYEDFTSFSK
                     FNKKRKTNLCTILDAGWLKDDHRLIFHITANRFLHDMVRAIVGTMMDVGHQKITLEEL
                     RQIIESKDRCRAGESVPAKGLYLTRVEYPVAIG"
     gene            complement(2693..3655)
                     /locus_tag="PKM34_05655"
     CDS             complement(2693..3655)
                     /locus_tag="PKM34_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ83109.1"
                     /translation="MAIYLNRGNVATKTGFGEGVREAARINPNVVGLGADITASVGMN
                     LFADEFPERFYSLGIAEQNCVGVAAGMALAGKVPVFSTYGVFAALRTTDFIRISLCYN
                     NLHAIIGGAHAGISVGPDGATHQALEDLSVMRSLPNMTVISPCDATQTRILTIKAINE
                     QNGPVYVRFGREPVPDFSNGNGKIEIGKSELLIDGEDATIIATGHLVWAAIQAAAILK
                     ESGINVRVLNMHTIKPIDKEAIIEAAVETGRIVTAEEHQVTGGLGSAVAEVIAQRYPV
                     PVRFVGMPDCFGESGKPDELMKKYGMSVEAVCEAIRSLPGIPRG"
     gene            3774..4874
                     /locus_tag="PKM34_05660"
                     /pseudo
     CDS             3774..4874
                     /locus_tag="PKM34_05660"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
     gene            4941..>5318
                     /gene="selD"
                     /locus_tag="PKM34_05665"
     CDS             4941..>5318
                     /gene="selD"
                     /locus_tag="PKM34_05665"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNQ83110.1"
                     /translation="MRLTQFTHGLGCACKLRPQVLEELLKNLPAEIFPDVLVGNEQAD
                     DAAVYRINDQQAIVQTLDFFTPIVDDPYTFGAIAAANALSDIYAMGARPLFALNIAGF
                     PSNRLPVSVLSQILKGASDKAAEA"
BASE COUNT         1367 a         1365 c         1232 g         1354 t
ORIGIN      
        1 tcttttaatg cctgctttaa aaaaaatgac cggtatgaca ccctccgaat acaggcacaa
       61 gcataaaaac aatttctcaa aataaccacc atacacccac tgcgattgcc agggcgccag
      121 cgattatcct caggttccgg aatgcggcag aataggcgaa attcgctgtt ttttgcccga
      181 tatatcccat gataaatgcg tatatgatca tcgcagagat tgtccccgct gcgaatcccg
      241 ccaggtagat aaccgactcg ctgacagaag gcaatgccag tgtaggcaga atcagcagaa
      301 aatgagaaat tccggcaaat ccatgcagcg ttccgatcaa taatgcagtt atgctatttt
      361 gaacagtaga ttttgagtga atgtgggtat gattggtttc gtcatgatga tcgtgacgat
      421 ggatatggac ctgtggagat ggtttttcgt ggtaatgagg atgggaatga tggaaatgaa
      481 accgtcccag cgttttgaca atggcccagg cgccaattcc gatcaataca aatccgacca
      541 gaaactcact gtaacccgaa atcttttcca cgttgatcat agatttgaat gccaggtaga
      601 gaagcccgat aagcaacatc ccggcaacat ggccgatgcc ccacatcaga ccgatattcc
      661 atgctttttt gcggttctct atggccagcg gggtcaccgc tgccaggtgg tcgggccctg
      721 tcacaacatg cacggccgct gccagaaaac ccgtaagtaa ttgaatcata acttaaaaaa
      781 tgtctaatta atttttaacg taccagtacc ttctattact tcaaacaacc ctttcctgtc
      841 ataacccttc cagactgcaa gccccaactc tcgtatccgg acccagtatg ccggcgttag
      901 acctcccgat tcttttaaag cagccttgat ctggttttca atcccggcaa aaatgtctct
      961 ttttaatgtg agcctgatcg acttgtcgtg aagcgtaaag gtggcagcag gcaaaggact
     1021 ttcggattcg ccgattttga tcagtccatg gcccagtgtg gctcccgtgc tgacctgcag
     1081 tccatcgttc agacaactga ccggcggttt tgatccggca taagaggtta ttcgtaactc
     1141 atcatgcccc gcttccagga tttcttttgc atacaaaccc atcttggcgc ctatgattgc
     1201 ataaatcccc aggtgaccgt gaatttcact ggtaagtgcc accagccgcc actcctcttc
     1261 tccatatttt tttattgttt cagccatgat ttcctttaca tccctggcat aatcctcctg
     1321 atgaacagga aatccgctga aaattacccc actttgcccg gaatgcccgt gtatgtcctg
     1381 cccttttgtt gtcatcatta tcattatcaa taatatcgta aatatatctt tcataaccta
     1441 attccatcga acatcgacaa tcgacaatcg acaatctcta actcatcacc aactctccat
     1501 gaaagatccc tttcaggccg atgagtttgt tggcaaggtc ggtcagcctg tcagccctcc
     1561 ctttcaccgc aatggtctcc aggcaatgat gatgatcaag atgaacatgt tgtaccgaaa
     1621 ggatcaggtc gtgatagtca tgctgaatgt gagtcgaatt gtgctgaagg tcccttttat
     1681 gatgatcata aaccagcaca atggctcccg caacttcctg gttaccttcc cattgccttt
     1741 tgatgagatt tttccggatg agatggcgga tggcctgcga ccggtttggg aatttgttct
     1801 gccgggcata ttcatccaac gcctgaagca attcatcttc cagcgataca ccaaatcttt
     1861 ttatattcat ggtaacacga attttaaaat gataacacaa aagtaacaaa ttcgatgaag
     1921 catagcgatt gatcagcgga ttcctttaaa tttgaaacga ttttaaataa gatgcatcac
     1981 cggtacttta taaagctggc gtataacggc gcccgttatc atggatggca gatccaggac
     2041 aatgccatta ccgtgcagca ggttattacc gatgcggtta aattaatgtg gtccagggat
     2101 tttcggatga tagggtgtgg gcggaccgat accggtgtta gtgcaaagca gttctatgcc
     2161 catttcgatc tgccggatga aaagagcaga gaagagctat ccggcctggc tcaccgcctg
     2221 aaccggtatc ttccggaaga tatcgttatc tacgatatct ttcccgttcc acctgacctg
     2281 cattcacggt atgatgccgt cttcaggact tacgaatatc atattcacac acggaaggat
     2341 ccatttctga atccgatgtc gtggtatgtt tatcctgctc caggtgtcga tcgcatgaat
     2401 gaagcttgtc tggttttaaa agaatatgag gactttacga gtttttcaaa atttaataag
     2461 aaaaggaaaa cgaatttatg tacgattctg gatgccggct ggctgaagga tgaccacagg
     2521 ttaatcttcc atatcacggc taaccgcttt ttacatgata tggtccgcgc gattgtcggt
     2581 acgatgatgg atgtgggcca tcaaaagatc accctggaag aactcaggca aattattgag
     2641 agcaaagacc ggtgcagggc aggggagtcg gtcccggcca aaggcttgta ccttacccgc
     2701 gtggaatacc cggtagcgat cggatagctt cacagacagc ttcaaccgac atcccatatt
     2761 tcttcatcag ttcatcgggc ttacctgatt ccccgaaaca atccggcatt ccaacaaatc
     2821 tgaccggcac cgggtagcgt tgggcgatca cctcagctac ggcacttccc agcccaccgg
     2881 tcacctggtg ttcctcggcg gtcacgattc ggccggtttc caccgctgcc tcgataatcg
     2941 cttccttatc aatgggtttg atggtgtgca tatttaaaac cctcacgttg atacccgatt
     3001 ccttcaaaat tgcggcagct tgtatcgccg cccagacaag gtgcccggtt gcaatgatcg
     3061 tcgcatcctc cccgtcaatc aataattccg attttccaat ttcgattttc ccatttccat
     3121 ttgaaaaatc aggtaccggt tctcttccaa accttacgta taccggcccg ttctgttcgt
     3181 tgatggcttt tatcgtcagg atgcgcgttt gcgttgcatc acagggcgaa atgaccgtca
     3241 tattcggcaa cgaacgcatg acggacagat cctcaagggc ctggtgtgtg gcgccgtcag
     3301 gtccaacaga aattccggca tgagcccctc cgataatagc atgtagattg ttgtagcata
     3361 atgagatacg gatgaaatcg gtggtccgaa gcgcggcaaa aacaccatag gtcgaaaata
     3421 ccggtacctt gccggccaga gccatcccgg cagccacacc gacacaattc tgctccgcaa
     3481 tgccaaggga atagaaccgt tccgggaatt cgtccgcaaa caggttcatc ccaaccgatg
     3541 ctgtgatgtc ggcccctagc ccgaccacat ttggattgat ccgcgctgcc tcccgcactc
     3601 cctcaccaaa accggttttg gttgcaacat tgcctcggtt taaatatatt gccatcctta
     3661 taatatatga atcccgacga actatcggat cgtaaaatta gcaaatcctg aaatcatatt
     3721 aactttgaac ttttcacttt tcactttgaa ccttgaactt taaactaaac caaatggtat
     3781 atctcgatta caacgctacc acccctgtcc atccagccgt tgcggaggtg atacgtcctt
     3841 acttcgaaaa atttttcggc aatccttcca gcagccatgc ttatggtatc gaagcccggc
     3901 tggccgtaga aaaggcccga aaccaggtgt cgaaactgat tgggtgcaaa agctccgaaa
     3961 ttatctttac cagcggtggc agcgagtcga ataatatggc ggtcaaaggt gcggcattgg
     4021 caaacagggc caaaggcaac cacattatca ccagcagcat cgagcatccc gccgtgaccg
     4081 aagtttgccg gtatcttgaa aaacaaggct ttaagattac ctggcttccg gttgatgggt
     4141 atggattggt ttctccaacc gatcttgaaa aagccataac acccgaaaca atcctggtaa
     4201 ccgtgatgca tgccaacaat gaaaccggca ccattcaacc catctgggag ttggccgcca
     4261 tcgcccataa tcacggtatt cttttccata ccgacgccgc ccagtcggcc ggcaaaatac
     4321 ggatcgatga tctgggtgtc gatctgctgt ctgttgcagg ccataagctt tatgctccca
     4381 aagggatcgg ggcgctattc gtaagggcag gggtgaagct cgaaaaactg atccatggag
     4441 ccgaccatga acagaatctc cgggcgggca ccgaaaatgt agccctgatc gccggcctgg
     4501 gagaagcatg tgacatatcc ctgcgcgacc tggatgtcaa tgcaaagcat tcacacgggt
     4561 tacgagagat gctttatgag aagatcagga tggaaatccc gatggtacgc ttaaacgggc
     4621 acccgaaata ccgcctgccg aatacgctta gcctttcttt tccgggaatt gaagcgaata
     4681 ccatcctttc ggagttggcg gaggaggtag ctgcctctgc cggcgcagcc tgtcatgcag
     4741 gggaaaccag catgtcggcc acgctgaaag ccatgaatgt accggttgaa tatgccatgg
     4801 gcacgatccg gttctcgaca ggcaggtttc tgagtgaaca ggatgttgag cacgcagcag
     4861 cggctgttgt ccaggtcgtt agtcgtcagt cggcagttgg cagtaggcag ctggccgccg
     4921 aaggagaaga tttaaaagag atccggttga ctcaatttac gcacgggctg ggctgtgcat
     4981 gcaaattaag gccgcaggtt ctggaggaat tgctgaaaaa ccttccggct gagatttttc
     5041 ctgatgtttt agttggcaac gagcaggccg atgatgccgc cgtttaccgt atcaatgatc
     5101 aacaggccat cgtgcaaacg ctcgattttt tcacacccat tgtcgacgat ccgtacactt
     5161 ttggtgccat cgcagcagcc aatgccctta gcgatatcta cgccatgggc gcccggcctc
     5221 tgtttgccct gaatattgcc ggtttccctt cgaaccgcct tccggtcagt gtactctctc
     5281 aaattctaaa aggtgcatcc gataaagcag ccgaggcg
//
LOCUS       DAOQXS010000158         5304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15833_length_5304_cov_3.934464, whole genome shotgun sequence.
ACCESSION   DAOQXS010000158 DAOQXS010000000
VERSION     DAOQXS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15833_length_5304_cov_3.934464"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PKM34_05670"
     CDS             complement(<1..2212)
                     /locus_tag="PKM34_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83111.1"
                     /translation="MKSGILAFLIIASYCSAAQEVGIGQWRDHLPYDYATRVEEFHSA
                     VYCATPYSMFYFDRDDNRVQRLSKVNGLSDLGVSDISLNTDQDILVVSYSNTNVDLVM
                     PDLTIINIPDIKRKEILGNKTINSIMNYGKFAYLSCGFGIVVLNVEKREIKDTYYIGP
                     EGSRLNVFGMTYNDTAFFAATESGIFYAAIDDPNLAFYGNWHKIPDLPYPNGYYNLIA
                     NYGGRIIVNHADPDITGNNAYVFENGEWNLFEEAEPGRVYYNIRVSGDKLVVSYGYFV
                     IVFNSSLEQELKIYTYGESSPMPSDAMIGTDGYYWLADRSKGLVKVWGGGFNHEFIKP
                     SGAPTADIFDMACSGGKLYLVPGGLTSTWNNSWKGARLYSLIDETWKTYSSSNVPPLD
                     TLRDFVSVAVDPQNSNHVFAGSWNRGMAEFNEGELVVIHNSDNSGLAPNMGLGYPNVK
                     VGGIAFDSENNLWATSSGSEKILAVRRPGGVAAGEWEAYNLGSSTTGAEVKKVIVDSY
                     NQKWIIPRTTQTNPNYIFVFNENNDPGKQFRGLKSGAGYGNLPGTSVYAIAEDLEGEV
                     WVGTDEGVAVFYSPQDVLLGGNSDAEQILVNIDGYTQYLLETEIVKAIAVDPANNKWI
                     GTERAGVFLLSADGTKQIHHFTEDNSPLFSNNITSLTINGVTGEVFIGTAKGLISFKG
                     TSTGPPDPVTDSYYAYPNPVKPGYSGTIAIRGPIAGATVKITDIRGSLIYETMAE"
     gene            complement(2273..3265)
                     /locus_tag="PKM34_05675"
     CDS             complement(2273..3265)
                     /locus_tag="PKM34_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNQ83112.1"
                     /translation="MQLSDQNSENRWLVVVNPNAGKKRGEKDWPEISGLLAAAGIDFH
                     HEFTSHRNHAVELTCEFISKGFRKIIVVGGDGTLNEVVNGIFRQDEVEPNDITLGMIM
                     VGTGNDWGKMYNLKENYKDAIHIIREGNIFLQDAGVVIYLENDIEQVRYFVNSAGMGY
                     DALVVQMTNQGKEKGRGGTMAYMSSLLRGLFRYNLAYLDIEVDGKRVYKGKVFSMSVG
                     ICKYSGGGMIQLPFAVADDGLLDVTLFKKVSKLTVIRHLKKLYSGRFTHLPFVDTYQG
                     KTISVISAPHNPSFLETDGESLGNGPFTFHLIPRCLRVITAETRHPKSEKFLAE"
     gene            complement(3265..4926)
                     /locus_tag="PKM34_05680"
     CDS             complement(3265..4926)
                     /locus_tag="PKM34_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ83113.1"
                     /translation="MKSDKIIFWLYYFSLGLMLVSLPFSNFFMSVSQFCLVGTFILHG
                     MVKEDVVRFFSENNRVKQVLLFLPYGIFWILKSVAGKFKEFFRRENSPAWIFSSIYLL
                     HLVGLFFTTDFDYALKDLRIKLPLFIIPLMLSTTDLLNRKGFRFLMYLFSAAVFTGTL
                     ISTYHYLTMDFTDSRELSRYISHIRFSLLIDLAIFFLAYIAFRKSDIPRWPRYIMAAI
                     ALWMVAFLALAAFMTGLVVFFLTAAVVLLYFILTRQGIVLRLVTLSGIILIFTVAFLF
                     VMNIGRDVNRVYPVDEGSLEKTTALGNPYWHDLSNPQTENGYYVWHYVATDELREAWN
                     RRSQYSFDGKDKAGQEIRYTLIRFMTSKGLRKDAEGLGKMSDAEVAMVEDGTASIVYV
                     SKSNLYVRIYKIFWEYGRYQETHNASGHSVMQRLEFWKASVNIIRDNWITGVGTGDLE
                     KAFQEEYVKSGSLLDPEYRWRSHNQFLAIFASFGVLGFAWFLFSLLFPALRLGKFHDY
                     HYLSFFIIIMLSMLTEDTLETQAGVTVFAFFSSFYLFSKKFIDIL"
     gene            complement(4995..>5304)
                     /locus_tag="PKM34_05685"
     CDS             complement(4995..>5304)
                     /locus_tag="PKM34_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNQ83114.1"
                     /translation="LNHEAVSQDVIYKVMAFFQLYIIVFIFGAMILSFLGLDFESAIG
                     ASISALGNIGPGLGLVGPVGNYGFVPDAGKWLLSFMMLLGRLELFTVLLLITPYFWKR
                     "
BASE COUNT         1419 a         1312 c         1238 g         1335 t
ORIGIN      
        1 attcggccat ggtttcatag ataagagatc cccggatatc ggtgatttta acggtagccc
       61 ccgcaatagg cccgcggatg gcgatggtac cgctgtatcc cggttttaca ggattgggat
      121 aggcataata actatcagta accggatccg gagggccagt ggatgtgcct ttaaatgaaa
      181 tcagaccttt ggccgtaccg ataaacactt cgcctgtcac accgttaatg gtcaggcttg
      241 tgatattatt ggaaaacaag gggctgttat cctccgtaaa atggtggatt tgcttagtcc
      301 catcggctga aaggagaaat acgccagccc tttcggtgcc tatccacttg ttgttggcag
      361 gatcgactgc tattgctttg acgatttcgg tctccaaaag atattgggtg tacccgtcga
      421 tattgacgag gatctgctcc gcgtcggaat taccgcccag cagaacgtcc tgcggggagt
      481 agaaaacggc tacgccttca tcagtgccaa cccagacttc accttccagg tcttcggcga
      541 ttgcataaac actcgtaccg ggcaggtttc cgtaaccggc gcccgatttc aggccacgga
      601 attgtttccc cggatcgtta ttttcattaa aaacaaaaat atagttggga ttggtttgag
      661 tggtcctggg gataatccat ttctggttat aggaatccac gatcactttt ttcacttcgg
      721 cgccggtggt gctgcttccc aggttatatg cctcccattc acctgccgca acgccgccag
      781 gccgcctgac agccaaaatc ttttcagagc cggaagaggt tgcccataag ttgttctcgc
      841 tatcgaaagc gattcccccg actttcacat tcggatatcc cagccccata ttcggagcca
      901 ggccgctgtt gtcggagttg tggatcacaa ccagttctcc ttcattaaac tcagccattc
      961 ccctgttcca ggaacctgca aacacgtggt tgctgttttg cggatcgact gcgaccgaga
     1021 caaagtccct cagggtgtcg agtggcggga cattggagct gctgtaggtt ttccaggttt
     1081 catcgatcag cgaatacaat cttgcccctt tccatgaatt attccaggtg gaggtcaagc
     1141 cgccggggac caggtatagc tttccgccgc tgcaggccat atcgaaaata tcggctgtgg
     1201 gggcgccgct gggtttgata aattcatggt taaatcctcc tccccatact ttcacaagtc
     1261 ccttgctcct gtcggccaac cagtaatatc catccgtccc tatcatggca tccgatggca
     1321 tcgggctgga ttcgccgtaa gtatatatct taagttcctg ttccaggctg gaattaaaaa
     1381 caatgacaaa atacccgtaa gaaacgacca gcttatctcc gcttacccga atattataat
     1441 agacccggcc aggttcagcc tcttcgaaca ggttccattc cccgttctca aaaacatagg
     1501 cattatttcc ggtgatatca ggatcggcat gattgacgat gatcctcccc ccgtaattgg
     1561 cgatcaggtt gtaataccca ttaggataag gtaaatccgg gattttgtgc cagttaccgt
     1621 agaaagccag gttcgggtcg tcgatggcag cataaaaaat cccgctctct gtagcggcaa
     1681 agaaagcggt atcgttgtat gtcatcccga aaacattcaa ccggcttcct tccggcccga
     1741 tataatacgt atctttaatc tctctcttct ccacattcaa taccacaata ccgaatccac
     1801 aggataaata ggcaaacttg ccgtaattca taatgctgtt gatggttttg ttccccagta
     1861 tctcttttct tttgatatcg ggtatattga tgatggtcag gtcgggcatg accagatcca
     1921 cattggtgtt ggaataggaa acgacaagga tatcctggtc agtatttagt gagatatcgc
     1981 taacccccag gtcggacagt ccgttgacct tgctgagccg ctggaccctg ttgtcgtcgc
     2041 ggtcgaagta gaacatgctg tatggggttg cacaataaac ggcggaatgg aattcctcca
     2101 cccgtgtggc atagtcgtac ggcaaatggt cgcgccactg gccgattccc acttcctggg
     2161 ctgcggaaca gtatgatgca atgatcaaaa aagctaaaat gcctgatttc atgtgtttca
     2221 tagctaattt atcggggtgt atgcagataa ctgattttta acagatttat tattattccg
     2281 ccaggaattt ttcactcttc ggatgccggg tttcagcggt gatcacccgg aggcaccgcg
     2341 ggataagatg gaaggtaaaa gggccatttc ccagcgattc gccgtcagtt tcaagaaaag
     2401 atggattatg gggggctgag ataaccgaaa tggttttccc ctggtaagta tcaacgaagg
     2461 gcagatgggt aaaacggccg gaatagagtt tcttaaggtg ccttataacc gttagcttcg
     2521 atactttttt aaagagggta acatccagca aaccgtcgtc agcgacggca aaaggcaact
     2581 ggatcatccc gcctccgctg tacttgcaga tcccgacgct catgctgaag acttttcctt
     2641 tgtagaccct ttttccatcc acttcaatat cgagataagc aagattataa cggaacaatc
     2701 ccctgagaag gctcgacata taagccatag tgccaccccg tcccttttcc tttccctggt
     2761 tggtcatttg cacaaccagt gcatcgtatc ccatccctgc gctattcacg aaataacgaa
     2821 cctgttcaat atcattctca aggtaaatga ccactcccgc atcctgcaaa aaaatatttc
     2881 cctcccggat aatatgaata gcatccttat aattctcctt caggttatac atttttcccc
     2941 agtcattgcc cgtaccgacc atgatcattc ccagggtgat atcgttgggt tcaacctcat
     3001 cctgcctgaa gatcccgttt accacttcat ttaaagtgcc gtctccaccc accacaatta
     3061 ttttccggaa tcctttgctg ataaattcgc aggtcagttc gaccgcatgg ttacgatggg
     3121 acgtgaattc gtgatggaag tcaatgccgg ctgcggcaag aagaccggat atctcgggcc
     3181 agtctttttc acctcttttt tttccggcat tcgggttgac gaccacgagc caccgatttt
     3241 cgctgttttg atcactaagc tgcatcagag gatgtcgata aattttttac tgaacaggta
     3301 aaaggagctg aaaaaagcaa aaacagtaac gccggcctgg gtttccagtg tatcttcggt
     3361 gagcatcgac agcattatga tgataaagaa agaaagataa tggtaatcgt ggaacttgcc
     3421 gaggcgtaat gcaggaaaca gcaggctgaa caggaaccac gcgaagccca aaacaccgaa
     3481 agaggcgaaa attgcaagga actggttatg cgaccgccat ctgtactcgg gatcgagcaa
     3541 ggagcccgat ttgacatatt cttcctggaa tgctttttcc aggtcgcctg ttccaacccc
     3601 ggtgatccaa ttgtcacgga taatattgac cgatgctttc cagaactcca ggcgttgcat
     3661 caccgaatgc ccgctggcat tatgggtctc ctgataccgt ccgtattccc agaagatctt
     3721 gtaaatcctg acatataaat tggatttgct tacataaacg atgctggctg ttccatcctc
     3781 caccatagct acttcagcat cgctcatttt accaagccct tcggcgtctt tccgcaaacc
     3841 tttggaggtc atgaaacgga tcagggtgta tctgatttcc tggccggctt tgtcttttcc
     3901 atcgaaagaa tattggctgc gccggttcca cgcctccctc agctcatcgg tagccacata
     3961 atgccagaca taataaccat tttcggtttg aggattggat agatcgtgcc agtacggatt
     4021 gccaagggcg gtggtttttt caaggcttcc ctcatccacc gggtaaaccc ggttcacatc
     4081 gcggcctata ttcatcacaa ataaaaaagc tacagtaaaa atcaggataa ttccgcttaa
     4141 agtgaccagc cggagcacga ttccctgccg ggtcaaaata aaataaagga gaactaccgc
     4201 tgcggtaagg aaaaaaacga ccaggccggt cataaacgca gccagtgcaa ggaaggcgac
     4261 catccagagg gcgatggcag ccattatgta gcggggccac ctcgggatat cgcttttccg
     4321 gaaagcgata taggccagga aaaatatagc cagatcgatc agcaggctga accggatatg
     4381 ggaaatatat cgcgacaact cccggctgtc ggtgaaatcc atagtcaggt aatggtaggt
     4441 gctgatcagc gtaccggtaa aaacagcggc cgaaaacaag tacatcagga aacggaatcc
     4501 cttcctgttg agcagatccg tcgttgataa catcagcggg atgataaaca gcggcagttt
     4561 tatccggagg tctttcaggg cataatcgaa atcggtggtg aagaataatc cgaccaggtg
     4621 gagcaggtag atggaagaga agatccaggc gggagaattc tcacggcgga aaaactcttt
     4681 gaactttcct gcgactgact tcagaatcca gaaaatcccg taaggaagaa aaagaagaac
     4741 ttgtttaacc ctgttgtttt ctgagaagaa ccttaccaca tcttctttca ccattccatg
     4801 aaggataaag gttccgacca ggcaaaactg ggaaacgctc ataaaaaaat tggaaaacgg
     4861 cagggagacc agcatcagac ccagggagaa ataataaagc cagaagatga tcttatccga
     4921 tttcataagc gcttttcagg ggggtattta caacgaaggt aacaagtatt tccatggaat
     4981 cggtttctta tcgcttatct tttccagaag taaggggtga tcagtaacaa aacggtgaac
     5041 agttcaagcc tgccaagcag catcataaac gataacagcc attttccggc atcaggaaca
     5101 aaaccataat ttcctaccgg tccgaccaac cccagtccgg gaccaatatt acccagggcg
     5161 gaaatactgg cgccgattgc cgactcaaaa tcaagaccga ggaaagacaa tatcatggcg
     5221 ccaaagataa agacgataat gtacaactgg aagaatgcca tcactttgta gatgacgtcc
     5281 tgtgaaaccg cctcatggtt tagc
//
LOCUS       DAOQXS010000159         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15851_length_5300_cov_3.341468, whole genome shotgun sequence.
ACCESSION   DAOQXS010000159 DAOQXS010000000
VERSION     DAOQXS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15851_length_5300_cov_3.341468"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKM34_05690"
     CDS             complement(<1..293)
                     /locus_tag="PKM34_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNQ83115.1"
                     /translation="MSIPMLIIFGVFMLLSWLISMQLKSKFKKYSTVPINQNLTGKEV
                     AEKMLRDNGISDVKVVSTPGMLSDHYNPLTKTVNLSEGVYASSNVAAAAVAAH"
     gene            complement(397..2088)
                     /locus_tag="PKM34_05695"
     CDS             complement(397..2088)
                     /locus_tag="PKM34_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ83116.1"
                     /translation="MKNLSGCIIWYLFIFNCLNIHAQTIIPGGYVSGNWDISGSPYLI
                     DGNLLVQPDSTLEIGLGTEILFRGTFRLEVLGQLLVSGTPTQPVIFDGENDTIEWRGI
                     YFNATDSSLTDSSILANGSINNSLGGSGLTLNNSNRVRISGFTIRNCASFQGGAIRCV
                     DSAPVFENLLIENNSALDGAGIALDASNAIIKNCSFNNNIANGAGGGMVIYNGSAPVL
                     ENCTFTGNSSIGSGGGIYINDASPVLSQCTFLANAGALGGSSLYSGGAVSVKLGADPR
                     FENCTFSENTSNTSGGAIASFSPNEIINCLFVENSAPVSGGAIFLSSGNPIETTLFNN
                     TFHNNESPDGSVLYAHNHVAVMLNCIAWQDETPDTSSLVRLDAIMSLVALFIAYSDLK
                     DAQEGIELTGNAGYSWGTGNIVLDPLFLPGIYDLDWQSPCIEAGTPDTTGLGLPELDL
                     NNNPRYANDRIDMGAFEYQLPLAVQSLKFKDQREMRVFPNPAREWVWVETNEDREYCS
                     IELINSSGLILSKKDLHPGEEMARIDLSPYPAGIYLVRLSANGEGVSNRSIIKVR"
     gene            complement(2361..>5300)
                     /locus_tag="PKM34_05700"
     CDS             complement(2361..>5300)
                     /locus_tag="PKM34_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNQ83117.1"
                     /translation="TGVGYDALSSITTGGNTAYGSSALKSNVDGIFNVAVGTSSQRNG
                     TTGSRNTSLGYGSLMNTRGSYNTGLGFSALSSDTTGIYNTAVGYNALMISRSSSYNTA
                     LGGYALYRNHSGTYNVAMGFSALHNDSVGNFNVAVGSNAIYYNIDGSRNTAVGNEAMA
                     GNSTGSGNVAIGNAALHSNEEINGLVAIGDSALYNNGIGASGDQAKGNTAIGAGSMYA
                     NTKGWANTSLGSKSLKQNKTGAKNVALGEQALYSNVAKSGNTAVGMQAMSHYDTTSTY
                     EFTFNTAVGYLALSGYDNPDEQHTGKYNTALGAGALFNNTTGNYNLAAGMNTSASNLT
                     GSYNCALGNRTLLANTEGSFLTALGYESMPNNTASYSTAIGYQAMYKTTTGGSNLALG
                     YQSLYHNKGNKRSTAIGILAMYYADSTDSGNDTYNTAIGYEALKGSTVPLNNTGRYNT
                     ATGDQAMYYNASGDSCTANGYRSLYANTSGKQNTAFGSAALYANTSGGYNTAAGNGAL
                     YNNKANSRSTAIGYRAMYYAFNQNASVRETFNTALGYEALRGSTSAASNSGQYNTAIG
                     DAALWTNSMGNHNTSVGAQALYQNTQGEQNTAMGFGALYNNKENDRSTAVGYMAMYNA
                     DNRTTSERSTYNTAVGFQALSGSSTPSANTGQENTAVGDQALSLNASGSFNTGCGAFA
                     LQFNDAGNYNTAVGNMSMVSNNGGSYSTAVGYQAMYATTTGGSNLAVGYRSLYSNVGK
                     RRNTVVGIEAMFNADDTPTSGDAYNTAIGYKALYGSGTPASNTGTSNTAVGDQALFGN
                     TSGNNNVALGRNALDGNTVGSNNTAIGYEADVAFNNLTNATAIGYGASVNASNKIVLG
                     NSSATTVGGYGTWTNYSDARLKENISYKDDLGLDFVMKMRTASYNYIDDENKRRRDGL
                     IAQDVQQALDELGVEFSGLVVDDDEMKTLNLSYGEFVIPLINAVKEQQAQIEALRKEI
                     EALKADR"
BASE COUNT         1320 a         1305 c         1221 g         1454 t
ORIGIN      
        1 tcatgcgcgg ccacagcagc ggcggcaaca ttgctgctgg catagacgcc ttcactgaga
       61 tttacggttt tggtgagcgg attgtaatga tcgctcagca tacccggagt cgaaaccacc
      121 ttaacgtcgg aaataccgtt atcgcggagc attttctctg cgacctcttt accggtaagg
      181 ttctgattta taggaaccgt cgaatatttc ttgaatttgg atttgagctg catggagatc
      241 agccagctca ggagcataaa aacgccgaaa atgatcagca tgggaatcga tatcatagtt
      301 ttaaaatttt aaatgaaaca agaaaccatc aattttgttg ccagatttat tcagattgtc
      361 tttcagacaa gttggcagta tgatatcttg caagaatcac ctcaccttta taatactcct
      421 gtttgaaacg ccttcaccat tggccgaaag ccttaccagg taaatccctg ccggataagg
      481 actcagatca attcttgcca tttcctctcc gggatgcaaa tctttcttac tcaatatcaa
      541 tcctgagcta ttaattaatt caatggagca atattcacga tcttcatttg tctcaaccca
      601 aacccactcc cgtgccggat tcggaaaaac cctcatttct ctttgatctt tgaactttaa
      661 actttgaact gccaagggca attgatattc aaacgctccc atatcaatcc ggtcgttggc
      721 ataccttgga ttattgttaa ggtcaagctc cggcaggcca agcccggtcg tatccggcgt
      781 tccggcttcg atacagggtg attgccaatc aagatcataa attccgggaa gaaacagggg
      841 atcaaggacg atgtttcctg tgccccagct ataacctgca tttccggtca gttcaatccc
      901 ttcctgagcg tctttcagat cggaatatgc gataaagagt gcaacaaggg acatgatggc
      961 atcaagacgg accagggaag aagtgtcggg agtctcatcc tgccaggcaa tgcagttcaa
     1021 catgaccgcg acatggttat gtgcgtatag cacagagccg tcagggcttt cgttgttgtg
     1081 gaacgtatta ttgaaaagtg ttgtttcaat tggatttccg gacgagagga atattgcgcc
     1141 gccgctaacc ggggctgaat tttcgacaaa aaggcaatta ataatttcgt tcggggaaaa
     1201 ggatgcaata gcaccacccg aagtgttgct ggtattttct gaaaacgtac aattctcaaa
     1261 tcggggatca gcgccaagtt tcacactgac ggcgcctcct gaatataagc tgctgccgcc
     1321 cagcgccccg gcatttgcaa ggaatgtaca ttggctcaga accgggcttg catcattgat
     1381 atatatccct cctccggagc caatcgaaga attgccggta aacgtacaat tttcaagtac
     1441 gggcgcactg ccgttgtaaa tcaccattcc cccgccggcg ccgttagcga tgttattgtt
     1501 aaaagagcag tttttaatga tggcatttga agcatccagg gcaatgccgg ctccgtcgag
     1561 ggctgagtta ttctcgatta gcaggttttc aaatacagga gctgaatcaa cgcatctgat
     1621 cgcacctccc tggaatgaag cgcaattccg tatggtgaag ccggatatcc tgaccctgtt
     1681 tgagttattt aaagtcaggc cgctgccccc gagggagtta ttgattgatc catttgcaag
     1741 gatggaggaa tcggttaaac tggagtcggt ggcattgaaa tagatcccgc gccattctat
     1801 ggtatcattt tcaccgtcaa aaatgacagg ttgggttgga gtccctgaaa ccagcaattg
     1861 accaaggact tccagccgga atgtgcccct gaaaaggatc tccgttccta atcctatttc
     1921 cagtgtggaa tcgggttgga ccaacaggtt tccatcgatc aggtaggggg aacccgaaat
     1981 atcccagttg ccactcacat acccgccggg gatgatggtc tgggcatgta tgtttaagca
     2041 gttgaaaatg aacaaatacc aaatgatgca gccggacagg tttttcattc ttgctatttg
     2101 tgaaataaga cagcaattaa atcagaatag ttgcaaagga tgaaaaaaga agaggctgct
     2161 cagaaatatg agacagcccc ttacagcaga attaacctac ttaactcacc gcatatcaaa
     2221 caaattccaa attccaagca ccaaaagcta aacaaattcc aaatgccaag caccaaaagc
     2281 caaacaaatt ccaaatctcc aaatcttcaa atcttcaaat cttcaaatct tcaaatcctc
     2341 aaatctccaa atctccaaat ctatcggtct gccttcagcg cttcgatctc cttcctcagc
     2401 gcttcgatct gagcttgttg ttctttcacg gcgttgatga gcgggatcac gaattcgccg
     2461 taagagaggt tcagggtttt catttcgtcg tcatccacca caagcccgct gaattctacg
     2521 cccagttcat ccagggcctg ttggacatcc tgtgcgatca gcccatctct tcttctttta
     2581 ttttcatcat caatataatt gtaagaagca gttctcatct tcataacaaa atcaagcccc
     2641 aggtcatctt tatacgaaat attctctttg agccttgcat cggaataatt ggtccatgtt
     2701 ccgtaacctc ctaccgttgt agcgctggag ttacctaaaa cgatcttatt gctggcattc
     2761 accgatgccc cgtagccgat ggcggtggca ttggtcaggt tattgaacgc tacatcagcc
     2821 tcgtatccga tcgccgtgtt gttcgagcca acagtattgc catccagggc attgcgaccg
     2881 agtgctacat tgttgttgcc ggaagtgttg ccaaacaggg cctggtcgcc caccgccgtg
     2941 ttggatgtgc cggtgtttga tgcgggagta ccgcttccat acaaggcctt ataacctatg
     3001 gctgtattgt aagcatcacc gctcgtgggc gtatcatccg cgttaaacat ggcctcgatg
     3061 cccaccacgg tattccttct tttcccgaca ttgctataaa gcgaccggta gccgacggcc
     3121 aggttcgacc cgccggtagt cgtagcgtac atagcctgat aacccacggc tgtcgaatat
     3181 gatcctccgt tattgcttac catcgacata tttccgaccg ctgtattgta gtttcctgca
     3241 tcattgaact gcaatgcaaa ggcgccgcag ccggtattga agctgccgga tgcgttcagg
     3301 ctgagggcct ggtcgccaac ggcggtattt tcctggccgg tattggctga aggagtgctg
     3361 ctgccgctga gtgcctggaa accgacggcg gtattgtagg tagatctttc actcgtggta
     3421 cggttatcgg cgttgtacat ggccatataa ccaacagcag tgctgcggtc attttcttta
     3481 ttgttgtaaa gcgcgccgaa tcccatggcc gtattttgct ctccctgagt attctggtat
     3541 aaagcctgcg caccgacact tgtattatgg tttcccatac tgttggtcca gagagcagca
     3601 tcccctatag cggtattgta ctgcccgctg ttggaagcgg cgctggtgct gccgcgtaaa
     3661 gcttcataac ccaacgcggt attgaaggtt tccctgaccg atgcgttctg gttaaaggca
     3721 taatacatgg cccggtagcc gatggctgtg ctgcggctat tggccttgtt gttgtatagt
     3781 gcaccattgc cggctgctgt attgtatccg ccacttgtat tagcataaag ggctgcactt
     3841 ccaaaggccg tattttgttt gccggatgtg ttggcataga gcgaccggta accatttgcc
     3901 gtacatgaat ccccggatgc attgtaatac atggcctggt cgccggtggc ggtgttgtag
     3961 cgaccggtat tgtttaatgg aacagtacta cccttaagtg cctcatatcc tatggctgta
     4021 ttatacgtat cattgccact gtcagtggag tcggcgtaat acatcgccag gataccaata
     4081 gctgtgctgc gtttgtttcc tttgttatga tacagcgatt gatagcccaa agccagattt
     4141 gatcccccgg tagttgtttt atacatagcc tgatatccaa tggcagtaga ataggaagca
     4201 gtattatttg gcatggattc gtatcctaat gcagttaaaa agctcccttc agtattagca
     4261 aggagggtgc gattgcccaa agcacagttg tatgacccgg taagatttga agccgaagtg
     4321 ttcatgccag cagcaagatt ataatttccg gtggtgttat tgaacagtgc tcccgcacca
     4381 agggctgtat tgtattttcc agtgtgttgt tcgtcaggat tatcataacc tgataaagcc
     4441 aagtatccaa cagccgtgtt aaaagtgaat tcgtacgttg atgtggtgtc ataatgcgac
     4501 atagcttgca ttccaacagc agtattaccc gactttgcta cattactgta taaagcctgt
     4561 tcacccaggg ctacattttt cgcaccggtt ttgttttgct tcaacgattt tgatccaagg
     4621 gaagtattag cccagccttt agtattggca tacattgatc cggcacctat ggcggtgttc
     4681 ccttttgcct gatctccaga tgcacctatt ccgttattat aaagcgccga atcaccaata
     4741 gccaccaggc cgtttatttc ttcattgctg tgcagtgccg cattacctat cgccacattg
     4801 ccactccctg ttgaattccc tgccattgct tcgtttccga cagccgtgtt acggctgccg
     4861 tctatgttat aatatattgc attagagcct actgcaacat taaaattacc gacagaatcg
     4921 ttgtgcaaag cgctgaatcc catcgcaaca ttataggtcc cgctgtgatt acggtacagg
     4981 gcataccccc ctaaagcggt attatatgag ctactgcggc tgatcatcaa agcattataa
     5041 ccaactgccg tattgtaaat acccgtcgta tcggatgata aagcactgaa cccgaggccc
     5101 gtattgtatg atccccgggt attcatcagg gaaccgtacc ccagggatgt gtttcgggaa
     5161 ccggtcgtgc cgtttcgctg agagcttgta cccacggcca cattgaaaat gccgtcgacg
     5221 ttacttttaa gggccgaact tccgtatgcc gtattgccgc ctgttgtgat tgaggatagc
     5281 gcatcatacc ccacccccgt
//
LOCUS       DAOQXS010000160         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15895_length_5286_cov_3.797744, whole genome shotgun sequence.
ACCESSION   DAOQXS010000160 DAOQXS010000000
VERSION     DAOQXS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15895_length_5286_cov_3.797744"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKM34_05705"
     CDS             complement(<1..583)
                     /locus_tag="PKM34_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83118.1"
                     /translation="MAEEFKHIDEIIRQKFDNFEPEPPLHVWENIKSSLPKDPAPPSS
                     PGILLPIIVTISLIIFIAGLFNQYYPDRPDTTAGESSSPYSTVQTAGVAPAGTTSNAN
                     LTLPEPAFQSPEIIPSPEEKKVKAEAKKQVPDIPVRAPFGENTKFSRKSKETRTVETT
                     PKLRKEPVAGYRPGIIRAVTLGTITYADAMRYDL"
     gene            complement(592..1122)
                     /locus_tag="PKM34_05710"
     CDS             complement(592..1122)
                     /locus_tag="PKM34_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ83119.1"
                     /translation="MYSDKELIEHCLANDPRAQEFLYKRFSRRMYGVCLRFARNTLEA
                     DDILQEGFIKVFAHLKDFRCDGSLEGWVRRVIVNTAINYYNSRLHELNETPIDKAVIM
                     NSTTEDILDKISAGDLLHLIQGLPEGYRMVFNLYVIEGYNHQEIAEMLKISENTSKSQ
                     LSRARTALQERLAQRK"
     gene            complement(1384..4815)
                     /locus_tag="PKM34_05715"
     CDS             complement(1384..4815)
                     /locus_tag="PKM34_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ83120.1"
                     /translation="MPVNSQPEKSQKVIRQFGLTNLALKNKNSIYLLILILFGFGFYS
                     YTSMPKELFPDIVIPTVLVQTAYPGNPPLDMENLVTRPIEKELEVITGVKEIASTSSQ
                     DISSIVVEFNTGVDLKQAVLDVKDAVDKAKSELPDDLPADPLVQDIDFSEFPIIYINL
                     SGDYSLNELKKYAEYLEDEIETYSEISKVDIKGLNEREFSINVDPFKMEMLEISFGDI
                     ESAVGFENISMAAGQFREGDYRRSIRILGEFETAAQMGDIIVKSEKGNIVYLKDIAVV
                     TDGFAEPKDFSRLNRQPVVTLHVIKKGGENLLSATDKIFDLLDRSKEDGNLPEGLNIT
                     ITNDQSDLIRKQLDNLENSMIMGVILVVFVLFLFLGTRNALFVGFAIPLSMFISFLIL
                     NLMGYKLNMILLFSLILALGMLVDNAIVVMENIYRYFDRGYSRMEASRLATGEIAIAI
                     ISSTATTLAAFFPLLFWDSMIGEFMKLLPLTLIIVLTASLFVALTVTPVLTSSFLKQG
                     DQLPKPKIKRSLIIVAILLLLAGIFYAGGMNTPGSLFVLFAIIGIANLLFLNRMARWF
                     MNVFLPWLEDAYLNTMNFVMKARRPYWVIAATFFLLIFTIVLMGIRQPKVIFFPSPDP
                     SYINIMATLPVGTDITATNKFMGTFEEDVYSILTPYSRIVKSVLTTVGNGAKSENEGF
                     DISETPHRGMVTVTFVDFEFREGVNTNDILKAFSDNLIGKYPGVMVAVDKPSSGPPVG
                     KAINIEVTGEDFDKLVILVDDLLNKIDNAGIDGIEGLKTDLNLDKPEAIVHIDRDRAR
                     RYGLSTAQIAGTIRTALFGKEISDFKEGEEEYPIQLRLKEEFRYNVASLMNQKITFRD
                     QASGKIQQVPITAVADISYGSTYEAIKRKDLDRVITLSSNVLKGFNATDINNQLKEFL
                     SGETLPSGYTYKFTGEQQEQDESMAFLGSAMLIALALILLIMVSQFNSVVKPVIIMIS
                     VLFSTIGVFGGLATFRMDFVVIMTGIGLVSLAGIVVNNAIVLIDYTDLLKLRKRTELG
                     MEENALLPKDIALDCVIQAGRTRLRPVLLTAVTTLLGLIPLASGLNIDFLSLFEHFDP
                     KIYFGGDNVAFWGPISWTIIFGLTFSTFLTLIVVPAMYHVLYLGKIRIHEMRKRK"
     gene            complement(4898..>5286)
                     /locus_tag="PKM34_05720"
     CDS             complement(4898..>5286)
                     /locus_tag="PKM34_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ83121.1"
                     /translation="ESVFGEYVTQYIRKGANLIFVITNDGWWGNTPGHRQHLTFSKLR
                     AIETRRSIARSANTGISAFIGQRGDIYQRTNYWEPAVIRQEINANNFTTFYVRYGDYI
                     ARLSAFIAVFVLLISISTRLRKKVEA"
BASE COUNT         1379 a         1251 c         1267 g         1389 t
ORIGIN      
        1 tcaggtcata tctcatcgcg tcggcataag ttatggtacc gagggtaacg gccctgataa
       61 ttcccgggcg gtatccggca accggttctt tgcggagttt cggtgttgtt tcgacggttc
      121 gtgtctcttt tgatttcctg ctgaatttgg tgttttcacc aaacggagcc ctgacaggaa
      181 tatccggaac ctgctttttg gcttcggctt tgactttctt ttcttccggg gaaggtataa
      241 tttccggtga ctggaatgcc ggctcaggca gggtcagatt ggcattggag gtcgtccctg
      301 cgggagcaac accggcggtt tgtacagtgg aatagggtga agaggattcg ccggccgtag
      361 tatcgggcct gtcggggtaa tactggttga acagcccggc aataaaaata ataagcgaaa
      421 tagtgacaat gataggaagc aggatccccg gggatgaagg aggtgcaggg tctttcggca
      481 ggctggactt gatgttctcc cacacatgca atggcggctc cggctcgaag ttgtcgaact
      541 tctgccggat gatctcatca atatgtttaa actcttctgc catcactttt actatttgcg
      601 ttgagccagt cgttcctgga gtgccgtcct tgccctcgat aattgtgatt tggacgtatt
      661 ctccgaaatt tttagcattt ctgcgatctc ctggtgatta taaccttcaa taacgtaaag
      721 gttgaaaacc atccggtaac cttccggcag cccttgaata aggtgaagaa gatctcctgc
      781 tgaaatttta tcgaggatat cttccgtggt tgaattcatg atcaccgctt tatcgatcgg
      841 tgtttcattc aattcatgca gcctggaatt gtaataatta atagctgtat tgacgattac
      901 tctccggacc cagccttcga gcgaaccgtc gcagcggaaa tctttcagat gtgcaaagac
      961 tttgataaat ccttcctgaa ggatatcgtc ggcttccagc gtgtttcttg caaaacgaag
     1021 acagactccg tacatgcggc gtgaaaaccg cttgtaaagg aactcctggg ctctgggatc
     1081 attcgccagg caatgttcga ttaattcctt atcagagtac atcttaactg aaaaacctgc
     1141 ttgaggccag tctttccaac tgtctgaagc tgattatcct aatttatctg ctacaaactt
     1201 agtaaataaa ttatgataca tcgcataaac agctattatt tttctgctga cacagaaacg
     1261 caatcgctca aagaacaact tcaacgcaac atcacaggca taaatttgcc cgtggatgca
     1321 aggagaagac agaataaaac gggaaagggt tgcacgggcc agaaaaaaat cccgtgaaaa
     1381 acactatttc cttttcctca tctcatgaat ccttatctta cctaagtaaa ggacatgata
     1441 catagcgggc acgacaatca gtgtcaggaa ggtgctgaat gtcaatccga agataatggt
     1501 ccaggaaatc ggcccccaga aagccacgtt atctcctcca aaatagatct tgggatcaaa
     1561 atgctcaaag agggaaagaa aatcaatatt cagtcctgat gccagcggta tcaatcctaa
     1621 cagagttgtt accgccgtta gcagaacagg gcgcaaacgg gtccggccgg cctggatcac
     1681 acaatccagc gcaatgtctt tcggcaacag cgcgttttcc tccataccga gctcggtgcg
     1741 ttttctcagt ttcagcaggt cagtgtaatc gatcagcaca atggcattat tgaccacaat
     1801 tcctgcaaga gaaaccagcc cgatccccgt catgatcaca acgaagtcca ttctgaacgt
     1861 tgccagcccg ccgaacacac caattgtcga aaataataca ctgatcatga taatgaccgg
     1921 cttgaccacc gaattgaact ggctgaccat gatcaggagg ataagcgcca gggcaatcag
     1981 catcgcgctg cccagaaagg ccattgattc gtcctgttcc tgttgttcac cggtaaattt
     2041 gtaagtgtaa ccggaaggga gggtttctcc tgacagaaat tctttgagct gattgtttat
     2101 atcggtagcg ttgaaaccct tcaatacgtt agaactcaag gtgatgaccc tgtccagatc
     2161 cttacgcttt atcgcttcat aggtgctgcc gtaactgata tcggccactg cggtgatcgg
     2221 tacctgctgt attttcccgc tggcctggtc cctgaaggtg attttctgat tcatgaggct
     2281 cgccacattg taccggaatt cctccttaag ccttaattgg attgggtatt cctcctcacc
     2341 ctccttaaaa tcggaaatct ctttcccgaa taaggccgtc cgtatcgttc ccgctatctg
     2401 ggccgttgat aaaccatatc gcctcgcccg gtcacggtcg atatggacga tggcttccgg
     2461 cttgtccagg ttgagatcgg ttttcaatcc ttcaatgccg tctataccgg cattgtcaat
     2521 tttattgaga aggtcatcga ccaggatgac cagtttgtcg aaatcttccc cggtaacctc
     2581 tatattgatg gctttcccta ccggagggcc gctgctgggt ttatcgacag ccaccattac
     2641 ccccgggtat tttccgatca gattatcgga aaatgctttt aaaatatcgt tggtgttaac
     2701 gccttcccgg aattcaaaat cgacgaaggt aacggtcacc attcccctgt gcggcgtttc
     2761 ggaaatatcg aacccttcat tttcactttt ggccccgtta ccgaccgttg tcaataccga
     2821 tttgacgatc cggctgtaag gggtcagaat gctgtaaaca tcctcttcaa aggtccccat
     2881 aaatttattg gtggcagtaa tatcggttcc taccggtaaa gtagccataa tatttatata
     2941 agaaggatcg ggagagggga aaaagatcac tttcggctgg cggattccca tcagtacgat
     3001 agtgaatatt aacaggaaaa atgtcgcagc gatcacccag taaggcctcc tggctttcat
     3061 cacaaaattc atggtgttca ggtaggcatc ttccagccag ggcaagaaaa cattcatgaa
     3121 ccaacgggcc atcctgttca ggaagagcag gttggcaatg ccgatgatgg caaataaaac
     3181 gaacagtgac ccgggtgtat tcatcccgcc ggcatagaaa ataccggcaa gcagaagcag
     3241 aatagcaaca atgattaagg aacgtttgat ttttggtttt ggcagttgat ccccttgttt
     3301 caggaatgag gaggtaagaa ccggtgtaac ggtcaaagct acaaaaaggg atgcagtaag
     3361 tacgatgatc agcgtgagcg gaagcagctt cataaactcc ccgatcatgc tgtcccagaa
     3421 cagcagcggg aagaaagcgg ccagggtggt ggccgtcgaa gagatgatgg cgatggcaat
     3481 ctcacctgtc gccaggcggc tggcttccat ccggctgtag cccctgtcga aatatcggta
     3541 aatattctcc atcaccacaa tagcattgtc gacaagcatt ccgagcgcaa ggatcaatga
     3601 gaagaggagg atcatgttca gtttgtaccc catcaggttc aggatgagga aagagatgaa
     3661 catcgataac gggatggcaa agccaacgaa aagggcattg cgtgtaccca ggaaaaggaa
     3721 aagaacgaac accaccagga ttacccccat gatcatgctg ttctcaaggt tgtcgagctg
     3781 cttgcggatc aggtccgact gatcgttggt gatcgtgata ttcagtcctt ccggtaaatt
     3841 gccgtcttcc ttggagcggt caagcaggtc aaagatcttg tcggtggccg ataaaaggtt
     3901 ttctccccct tttttaatga catgcagcgt cactaccggc tgccggttca accttgaaaa
     3961 atctttgggc tctgcaaacc cgtcggttac aaccgcaatg tctttcaggt aaacaatatt
     4021 ccctttttcc gatttgacga tgatgtcgcc catctgggct gccgtttcaa attctccgag
     4081 gatgcggatc gaccggcggt agtcgccttc gcggaactgc ccggctgcca tggagatatt
     4141 ctcaaacccg actgcgctct caatatcgcc aaagctgatc tccagcatct ccattttaaa
     4201 aggatcgaca ttaatactga actccctctc atttaatcct ttaatatcga cttttgaaat
     4261 ttcggaataa gtttctattt catcttcgag atattcggca tacttcttca gttcattcag
     4321 gctgtaatca cccgacaggt tgatgtaaat gatcggaaac tccgagaaat cgatatcctg
     4381 aaccagcgga tcggcaggca ggtcatcagg cagttcactt ttggctttgt cgacggcatc
     4441 ttttacatcg agcacggcct gcttcaaatc gacacccgta ttgaattcca cgacaatcga
     4501 agaaatatcc tgggaagagg tgctggcaat ctctttcact ccggtaatta cctccagttc
     4561 tttctcaatg ggccgcgtga caaggttttc catatcgagc ggagggttcc cggggtacgc
     4621 ggtctggacc agtacggtcg ggatcacaat atccgggaaa agttcttttg gcattgaagt
     4681 gtaggaataa aaaccgaatc caaaaaggat caggatcagc aggtaaatcg agtttttatt
     4741 cttaagggcc aggttcgtca ggccgaactg tcggatgacc ttctggcttt tttccggttg
     4801 tgaattaaca ggcataaatg attaacaatt caggtgatga agtcggattt aagacggtgc
     4861 aaaagtacga cttggattga aaaattaaat ttttttatca ggcttctact ttttttctta
     4921 accgggtgct gatggagatc aaaagcacaa acacggcaat gaaagccgac agcctggcaa
     4981 tataatcgcc atatctaaca tagaaagtgg taaaattgtt cgcattgatc tcctggcgga
     5041 taacggcagg ctcccagtag ttggttctct ggtaaatatc gccacgctgg ccgatgaatg
     5101 cagaaatacc ggtattggcc gaacgggcta tgctgcggcg cgtttcgatg gccctgagtt
     5161 tactgaaagt tagatgctgc cggtggccgg gtgtgttgcc ccaccagccg tcgttggtga
     5221 tcacaaaaat aaggttggcc cccttgcgga tgtattgtgt gacatattcg ccgaagaccg
     5281 attcat
//
LOCUS       DAOQXS010000161         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15941_length_5274_cov_3.675608, whole genome shotgun sequence.
ACCESSION   DAOQXS010000161 DAOQXS010000000
VERSION     DAOQXS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_5274_cov_3.675608"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..367
                     /locus_tag="PKM34_05725"
     CDS             176..367
                     /locus_tag="PKM34_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNQ83122.1"
                     /translation="MNTGTVKFFNNSKGFGFIKDDASDKEYFVHVSGLKDEIKENDRV
                     TFDLQEGRKGLNAVNVKKS"
     gene            complement(442..1173)
                     /locus_tag="PKM34_05730"
     CDS             complement(442..1173)
                     /locus_tag="PKM34_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83123.1"
                     /translation="MKKILLSLFAVCFLLSANTQSFVLSYEGNTLAPDAVLVVADDPT
                     VEVIEAWVNITNNAATAKSVLVKKVIHDGDTLAGSDNTFCWGLCYPNTTYVSPFPQTI
                     QPGATSDLFYGDYRPNNLIGISKITYVFWDEMNVNDSVAVNVHYKASPASVNDLAAKI
                     MASGVYPNPASGMAYIDYSIPNSGGNNRLVITNMLGARIKEIGLKNISGKATLDVSEL
                     MNGIYFYSIISDNQQVITGKFLVRH"
     gene            1273..1539
                     /locus_tag="PKM34_05735"
     CDS             1273..1539
                     /locus_tag="PKM34_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83124.1"
                     /translation="MKTIYLALFAFSFLVFSVHSQSQRMVLIEEATNASCGPCASQNP
                     AFDNLLNQNRDILTAIKYHWYFPGYDPMITTIQLKTMHGSLITA"
     gene            1719..2966
                     /locus_tag="PKM34_05740"
     CDS             1719..2966
                     /locus_tag="PKM34_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83125.1"
                     /translation="MMIEATQAVSGTLIAHMVVVEKHINFTTPPGSNGEKNFHDVMKK
                     MIPDQFGTTLPASFQPGEYLILQGSWELANVYKIEELGVVGFIQNNVNKDVLQAANST
                     AEPFTPLYDHEASITKVSNVSETNCLGTVKPELTIRNNGAEPLTQVDVYYRINDETTY
                     LYEWTGNLGFLGSDVITLPEISFNIIDENSLYAYTVDPNGVPDEYIGNDTIIQSFSRA
                     EITPLTVKLMIRTDENPDETTWEIRNSAGELQFSGGPYSQPNTVIQETFQMEDMECYI
                     FRIFDEGGNGLNIPGFYALYHGSNSYISNGTDFGAIDTAYFEVNTQVGIDEMQSNPPM
                     VIFPNPARDKFTTTLWLDGQKEVRIFIYDIAGQLVTSVYAGQLMAGYQTVEINTKDLA
                     PGLYFVRTNIGPSHQTQKITIIK"
     gene            2976..4631
                     /locus_tag="PKM34_05745"
     CDS             2976..4631
                     /locus_tag="PKM34_05745"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83126.1"
                     /translation="MKGRFFLFTLALLLSGLIYGQTVPRDKVIVEIGTGTWCPYCPGA
                     AMGADDLIANGHEVAIIEYHNGDDYTNIYSNARNTYYQIPGFPTAYFDGGSAVVGGSG
                     TESMYPSYLPRVNQRLAIQSAFTLEVEGTHTCLTDFNAHVTLEKVGTNSSSNLRLHVV
                     VTESHIEKFWQGMDELNYVCRLMAPNQNGTTVSFSGGNTQVYDIPFTIDPSWVPEECE
                     VVVFLQDHSTKEIHQGTKLPLLDFLPVYQYDAMVKQVVDLPATSCMGSLEPTIKLRNV
                     GSESMTSVQIFYQVNGGETESYSWAGSLNYLDEEEVVLPEITFPAGHEYELVVYTSNP
                     NENDDECPSNDGKTIILPEAMNTPNTVKLLFRTDENPDESTWELKNGAGEVLYSGGPY
                     STPNQMIQETFELEQEACFSFHFYDEGGDGLITPGYYALYYGSNTVIQQGTGDFGYGL
                     SINFNTADPVGIDETPAKPSLTVFPNPFKDKTNIELTLAEAMPVSVSIYAITGLSVYE
                     SNEGTMDAGYHLITLDASTLRPGLYLYRVMAGETVYTGKLNIR"
     gene            complement(4624..>5274)
                     /locus_tag="PKM34_05750"
     CDS             complement(4624..>5274)
                     /locus_tag="PKM34_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNQ83127.1"
                     /translation="PEDLRTTDRIAAEVLEQLYSGAPPEIRMQLSDNILWIKSAEENK
                     LVVGSQARILYADAEGRIRIAEAFNRAIRDGLISAPVVLGRDHHDVSGTDSPYRETSN
                     IYDGSRFTADMAVQNVIGDSFRGATWVSLHNGGGVGWGEVINGGFGMLLDGSADASRR
                     LKNMLFWDVNNGIARRSWARNPEAVFAIRKAMEAEPLLKVTLPHMTDNDMIEKSFT"
BASE COUNT         1437 a         1268 c         1244 g         1325 t
ORIGIN      
        1 attatccaag cgatgtggcc ggcggtgttt tcatagggct tttcaccggc tggttcttct
       61 gccgcctgtt gaacaacaaa cggaccggcc ggcaggaaat taaaaaagat tgattatcac
      121 gttatcaatt caaaaaaaca tatctttgca aaaatttaat aatcaataca tcattatgaa
      181 cacaggaaca gtaaaattct ttaataactc caaaggtttc ggatttatta aagacgatgc
      241 atcggacaag gaatattttg ttcatgtatc aggattaaaa gatgaaatca aggaaaacga
      301 cagagtaacc tttgatcttc aggaaggcag aaaaggatta aatgcagtta atgttaagaa
      361 atcttaatat aaaaagcaca taaccttgat ctcaggaaag ccttacaaaa attgtaaggc
      421 ttttttgttt ggaattttat atcaatgtct tacgaggaac ttcccggtga tcacctgctg
      481 gttatcggag ataatggaat aaaaatagat cccgttcatc agttcagaaa catccagcgt
      541 tgctttgccg gatatgtttt ttaagccgat ctctttaatt ctagcgccca gcatattggt
      601 gatgaccagc cggttgttac cgcccgaatt tggaatggag taatcgatgt aagccatacc
      661 ggaggcggga ttggggtaaa caccggaagc catgattttt gcggccaggt cattcactga
      721 tgccgggctg gctttataat gcacattcac ggctacggaa tcgttcacat tcatctcgtc
      781 ccagaaaaca taggtgattt ttgatatacc gatcaggtta ttcggcctgt aatcgccata
      841 aaacaggtcg cttgtggctc ccggctggat ggtttgcggg aacgggctta cataagtcgt
      901 gttgggatag cacaaccccc agcagaatgt attgtcggat ccggccaggg tatcgccatc
      961 atgaatcact ttcttcacca gtaccgattt ggccgtggcc gcattattgg tgatattcac
     1021 ccaggcttca attacttcca cagtagggtc atcagcaact acgaggacgg catccggcgc
     1081 cagggtattc ccctcatagg acaggacaaa gctttgagta ttagcgctta acaagaagca
     1141 aacagcaaag agagagagta aaattttctt catgacaatc tttattacaa gtcttattta
     1201 attatgcaaa tatataacaa acgactaaaa tatttttaat tttacgtcct ttatttttca
     1261 aaaatcatac caatgaaaac aatctaccta gcattatttg cattttcttt tctggtattt
     1321 tcggttcatt cccaatccca gagaatggtg ctgatcgaag aggctaccaa tgcatcctgc
     1381 ggaccgtgtg cctcgcaaaa ccctgctttc gacaacctcc tcaaccaaaa cagggatatt
     1441 ctcaccgcta tcaagtacca ctggtatttt cccggatatg acccaatgat aaccacaata
     1501 cagttgaaaa caatgcacgg gtctcttatt acggcataaa tggggttcca acggccacca
     1561 tcgacggcga tattccaaac ggcccgactt tcagttatcc gggaggtccg cacggatata
     1621 cccaggaact gcttgacgaa tatgctgcca tcccttcacc gttcaatctc tggctcagcc
     1681 acaggatttc ggatgatgag gacagcatct acgtagatat gatgattgaa gcaacacagg
     1741 ccgttagcgg caccctcatc gcccacatgg tcgttgtgga aaaacacatc aattttacaa
     1801 cgcctcccgg atcaaacggc gaaaagaatt ttcatgatgt aatgaaaaaa atgatcccgg
     1861 atcaattcgg caccaccctc cctgcctcct ttcagccagg tgaatatctt atcctgcagg
     1921 ggtcatggga actggccaat gtttataaga ttgaagaact gggagttgta ggatttattc
     1981 agaataatgt gaataaagat gtcctccagg cagccaacag cacggctgaa cctttcactc
     2041 cgctctacga ccatgaagcc agcataacga aggtttcgaa tgtgagcgaa accaattgtc
     2101 tggggactgt caagcctgaa ctgacgatca ggaataacgg ggccgagccg ctaacccagg
     2161 tggatgtata ttaccggata aatgatgaaa cgacatacct gtatgagtgg accggtaatc
     2221 tcggcttcct gggatcggat gttatcacac ttcctgaaat cagcttcaat attattgacg
     2281 agaacagcct ttatgcatat acggtcgacc cgaatggcgt acctgacgaa tacatcggaa
     2341 acgatacgat catccagtct ttcagcaggg ccgagatcac tccgctgacg gttaagctca
     2401 tgatccggac agacgaaaac ccggatgaga cgacctggga aatccgcaat tcggctggcg
     2461 aattgcagtt ttccggcgga ccctattccc agccgaatac cgttatccag gagaccttcc
     2521 agatggaaga catggaatgc tatatcttcc ggatatttga cgaaggaggc aacggtttga
     2581 atatccccgg attctatgcc ttgtaccacg gatcgaacag ttacatttca aacgggaccg
     2641 atttcggtgc gatcgataca gcctattttg aagtgaatac gcaagtcgga attgacgaaa
     2701 tgcaaagcaa tcctccaatg gttatttttc ccaatccggc cagggataag ttcaccacaa
     2761 ctttatggct cgacggacaa aaagaggttc gaatcttcat ttatgatatt gcaggccaac
     2821 tggttacttc tgtgtatgcc ggccagctaa tggcaggtta ccagacggtt gaaataaata
     2881 ctaaagatct ggcgccggga ttatactttg tcagaaccaa catcgggcca tcccatcaga
     2941 cacaaaaaat taccattatc aaataacgaa aacatatgaa aggaagattc tttttattta
     3001 ctctagcgct cttactctcc ggccttattt acggtcagac agtaccccgc gataaagtga
     3061 ttgtcgaaat cggtaccgga acatggtgtc cgtattgccc aggggctgcc atgggtgctg
     3121 atgatctgat cgcaaacggg cacgaagtag ccatcattga gtatcacaac ggggatgatt
     3181 acacaaatat ttattccaat gccaggaaca cctattacca gattcccggt ttcccgacgg
     3241 cctatttcga cggcggcagt gcggttgtag gcggcagtgg aacggagagc atgtacccct
     3301 cctatctccc gcgggtcaat cagcggttgg cgatccagtc ggcgttcacc ctggaggtgg
     3361 agggaacgca tacctgcctg accgatttca atgcccatgt aacgctggaa aaggtcggta
     3421 cgaatagcag ctcgaacctc aggttgcatg ttgtggtgac cgaatcccat atcgaaaagt
     3481 tctggcaggg gatggacgaa ctgaactatg tttgccggct catggctccg aaccagaatg
     3541 gtacgactgt cagcttcagc ggtggcaata cgcaggtcta cgatatcccg tttacgatag
     3601 acccgtcgtg ggtgccggaa gagtgcgagg tggttgtttt cctgcaggat cacagcacca
     3661 aggagattca ccagggcacc aaacttcctc tgcttgattt ccttccggta taccagtatg
     3721 atgccatggt gaagcaggtt gtcgacctcc cggcgacatc gtgcatggga agcctggaac
     3781 cgacaattaa attgcgcaat gtgggttccg aatccatgac ttcagtacag atcttttacc
     3841 aggtaaacgg aggggaaacc gaatcttatt cctgggcagg gtctctgaac tatctcgacg
     3901 aagaagaggt cgttcttcct gaaattacct tcccggccgg tcatgagtat gaattggtcg
     3961 tttatacttc caatccgaat gaaaatgatg acgaatgccc ttccaatgac ggcaagacca
     4021 tcattttgcc cgaagccatg aacactccca atactgtcaa gctgcttttc cgcaccgatg
     4081 agaatccgga tgaatcaacc tgggaactga agaatggcgc cggcgaagtg ctctattcgg
     4141 gaggccccta ttctactccg aaccagatga tccaggagac tttcgaactg gaacaggaag
     4201 cctgtttttc tttccatttt tatgatgaag gtggtgatgg attgatcaca ccgggttatt
     4261 atgcacttta ttatggcagc aacacagtga tccagcaagg taccggcgat tttggctatg
     4321 gactgagtat caatttcaat acggccgatc cggtgggtat cgatgagact ccggcgaagc
     4381 catccctgac tgtatttccg aatcctttca aagacaagac caacatcgaa ctgacgctgg
     4441 cagaagccat gccggtttcg gtcagcattt atgcaataac cggtctatcg gtttatgaaa
     4501 gcaatgaggg aacgatggat gcgggatacc acctgattac gttggatgca agcacgctca
     4561 gaccgggact ttacctttac cgtgtcatgg ccggtgaaac ggtttacacc ggcaaactga
     4621 atatcaggta aaagactttt cgatcatatc attatctgtc atgtgaggca gggttacttt
     4681 cagaagtggc tctgcctcca ttgctttccg gatggcaaag acagcttccg gattccgtgc
     4741 ccagctccgg cgtgcaatgc cattattgac gtcccagaag agcatatttt tcaggcggcg
     4801 ggatgcatca gccgatccgt cgagaagcat gccgaatcct ccgttgatca cttcacccca
     4861 gccgacgccg cccccgttat ggagcgaaac ccatgtagcc ccccggaaac tgtcgccgat
     4921 aacgttctga acagccatat cggccgtaaa ccgggatccg tcatagatat tcgaagtttc
     4981 acggtaaggc gagtcggtac ctgaaacatc gtgatggtcg cggcccagga ccaccggggc
     5041 agagatcagc ccatcccgga tggcccggtt aaacgcttcg gcgatcctga tcctgccctc
     5101 ggcatcggca taaagaatgc gggcctgtga accgacgacg agtttatttt cttcggcaga
     5161 ttttatccag agtatgttat cgctgagctg catcctgatt tccggcggag cgcctgaata
     5221 aagctgttcg agtacctctg cggcaatcct gtctgtcgtt ctgaggtctt ccgg
//
LOCUS       DAOQXS010000162         5267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_15963_length_5267_cov_3.848427, whole genome shotgun sequence.
ACCESSION   DAOQXS010000162 DAOQXS010000000
VERSION     DAOQXS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15963_length_5267_cov_3.848427"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKM34_05755"
     CDS             complement(<1..728)
                     /locus_tag="PKM34_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83128.1"
                     /translation="MKKVLYFLLVLVIATSCYKQGQVDETQDKWNGYQKFLKTGEQVH
                     TLWAGRNINVGTVTYGLDDNANFYAKYDCSASGWTISETHMFAGDKLALPRNKPGNPK
                     IGHFPYSGCHNPRVSTYTYYVPLSTLPPCEEPGFVVSAHCVVRSPAGKNETAWAEGDY
                     KFTDKDWGWYDIYFYNQPPNEFTILYATSFTNDTLKLFHVDVTNGNTDLILKETTNTG
                     GRIDAAAYDALSGMFFFADYDYNTL"
     gene            complement(1111..2361)
                     /locus_tag="PKM34_05760"
     CDS             complement(1111..2361)
                     /locus_tag="PKM34_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83129.1"
                     /translation="MKKTVLFFTLALLMLACNKQNQLVDDGNKWNGYEKYLKMGEQVH
                     TLWAGKNINVGTATYGIDNNANFYVTYDCSASGWTMSETHMFAGDKAIMPLNKPGSPK
                     IGLFPNSGCHNPRVSTITYTVPLSTLPPCEEPGFVVASHCVVHSPNGKCETAWAEGDF
                     KFTDKGWGWYDVYYFNQPVNEYTILYGTNYTNDSLHLYHIDATNGGATPILSEYVGNT
                     GGSYDGAAYDTESTSFFFVKEAEPDQLWVNVLTDEAPSFYSGDLEGESASATYHDGTY
                     IYVDAATNTIKEVTLNEDLTKSGESVVNEIPGSVTVNDIAMSPDGETLYILGEVNGGG
                     KELISYDMGTETFYSMDISVTTGAQIAFGSDGVLYVVATIVEGGSHSLIYNLDPSSGT
                     LTLIEDDVIIIDDPFSDLSTGPIM"
     gene            complement(2651..3184)
                     /locus_tag="PKM34_05765"
     CDS             complement(2651..3184)
                     /locus_tag="PKM34_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83130.1"
                     /translation="MKKLITICMILSGILFAFPVLAQVNKNKPVNKESTSDKKPGTIT
                     ANTKGATATKMFDAAGTLIFTHDQMGYVRNARNRVFFQYTAAGDIIKKRAVIGSVKNG
                     IFRDRLGKEYARIVQDGKVVDANSKPVGTIKTDGTVLDKNGTKIGSAPGVNVNVAVMV
                     YFYRDVLENTKVKGSVR"
     gene            complement(3259..4506)
                     /locus_tag="PKM34_05770"
     CDS             complement(3259..4506)
                     /locus_tag="PKM34_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83131.1"
                     /translation="MKNLIEMMLVAVFMSGCAEIMTEEEMPDKWNGYFQYLKTGEMVH
                     TLWAGKNINVGTVTYGIDENANFYATYDCSAPGWKMSETHMYAGTKAGMPLNKPGRPK
                     IGQFPNSGCHSPRVSTFTYRVPLSSLPPCASPGFVVAAHCVVHGPGGKTESAWAEGSY
                     TFSDKGWGWYDDYYYDPPQEPGPVLYGTSCANDSLRIYHLDISTGCVEMILEEYVGGS
                     PGFYDGADYDPVTGLLFFIKYTTSELWINHLQGEEPSFCAGILSGPSTCGTFQGDHYY
                     YINNSTGSIQSVSFTSTWLIAEEMPLDTLPAGYAVNDIAMDSSGESLYILAQNQSGSM
                     QLLRWDAGEPAFFSMPVAVSEDAQIAFGSDGLLYAVSSTGECDTCFYIYTIDLENGML
                     EFVEDDVIMFDDPFSDLSRGPSL"
     gene            complement(4868..5188)
                     /locus_tag="PKM34_05775"
     CDS             complement(4868..5188)
                     /locus_tag="PKM34_05775"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ83132.1"
                     /translation="MADEKVINIIRRYVYLLDIEGLGIDKAFLYGSYASGSNSENSDI
                     DLMLVSGLISDNDIQKKSRAWILTKDVDTRIEPYLISTTRFTKDDISPLIEVVKREGI
                     EILL"
BASE COUNT         1401 a         1193 c         1194 g         1479 t
ORIGIN      
        1 agggtgttat aatcatagtc ggcaaagaag aacatgccgg acaatgcatc atatgctgct
       61 gcatcgatcc ggccgccggt attggtcgtt tccttcagga tcagatccgt atttccgtta
      121 gtcacatcca catggaacag cttcaaggta tcattcgtga aggaagtggc gtataaaata
      181 gtgaattcat tcggaggctg attgtaaaaa tagatatcat accatcccca gtctttatct
      241 gtaaatttgt aatcaccttc tgcccaggca gtttcatttt tcccggcagg gctgcgcacc
      301 acgcaatggg ccgaaacgac aaatccgggc tcttcgcatg gaggaagcgt gctcagcgga
      361 acataatagg tataggtgct gacccttgga ttatggcaac cggaataggg gaaatggcca
      421 atcttaggat tacccggttt atttcgcggt aaagcgagtt tatcaccggc aaacatatgg
      481 gtttccgaga tcgtccaccc ggaagcgctg cagtcatatt ttgcatagaa gtttgcattg
      541 tcatcgaggc cataagttac agtcccgaca ttaatgttcc tgccggccca taatgtatgg
      601 acctgttcgc ccgttttaag gaatttctgg taaccgttcc atttgtcctg ggtttcgtca
      661 acctgtcctt gtttgtaaca ggaggttgct atgaccagta ccaacaagaa atagagtact
      721 tttttcataa ttcagttttt aaaaagttaa ttttcgatga tttagtttgt tttcaattca
      781 attcaattca aaggacaaaa aatgataatc gttaccgata ccgacctggt tatacgggca
      841 ggtttcaata aggttttatt tttttcggga tggcggggaa atagcagaaa aaaaacagac
      901 ccaggaaaca ttaatcccgt tactattata aaggttacac gtggaaattg tgtaatgtga
      961 aaataaaaag cttatttgta acgaatataa ataattcgct gcaaataagc tccgtaataa
     1021 atgtgatgcg ggaaccgaca gctttagtcg tcggtctgat aacaatacag aggctgccca
     1081 gtctttttag acagcctctt ctatttattt ttacataatc ggtcctgtcg acaggtcgga
     1141 gaacgggtca tcgataataa taacatcgtc ttcgatgagt gtgagcgttc cactgctcgg
     1201 atccaggttg taaatgagag aatggctgcc gccttcaaca attgtcgcta caacatacag
     1261 aacaccatca ctgccaaagg cgatctgggc gccggttgta accgatatat ccatgctata
     1321 gaaggtttcc gtacccatat catatgatat cagctctttg cctccgccat ttacttcgcc
     1381 aaggatgtaa agcgtctcac cgtcggggct catggctatg tcatttaccg ttactgaacc
     1441 cggaatctca tttacaaccg attcgcccga ttttgtcagg tcttcgttta atgttacctc
     1501 tttgatagta tttgtggcag catcaacata aatataagta ccatcatgat aagtagcact
     1561 ggccgactca ccttcgaggt ctccagaata gaacgacggg gcttcgtcgg taaggacatt
     1621 gacccataac tggtccggtt ccgcttcctt tacaaagaag aaggatgtcg attctgtgtc
     1681 gtaagctgct ccatcatagc ttccgcctgt attccccaca tattcggata agatcggggt
     1741 ggcgccacca ttggtagcat cgatatgata aaggtgcaat gaatcattcg tgtaattggt
     1801 gccataaagg atcgtatatt cattgacggg ctggttgaaa taataaacat cataccatcc
     1861 ccagcctttg tcagtaaatt taaaatcgcc ttcggcccag gctgtttcgc atttgccgtt
     1921 tgggctgtgc actacgcaat gggaagcaac tacaaaaccg ggttcttcac atggcgggag
     1981 agttgaaagc ggtacagtat aggtaattgt gctcaccctc ggattgtggc aacctgagtt
     2041 tggaaacagg ccgatttttg gtgagcccgg tttgtttaac ggcatgatcg ccttatcgcc
     2101 ggcaaacatg tgagtttccg acatagtcca tcccgacgcg ctgcaatcat aagttacata
     2161 gaagtttgca ttgttgtcga tgccataggt ggcagttcct acattgatgt tttttccagc
     2221 ccacagggta tggacctgct cgcccatttt aaggtacttt tcatatccgt tccatttatt
     2281 gccgtcgtcg actaattgat tctgtttgtt gcaggccagc ataagcaggg caagagtgaa
     2341 gaataatact gtctttttca tatcgctgag ttttggttag ctagattttg ggctaagaac
     2401 atgagcagca attctccccc ggttaattat acgggccgga aaaatgccat gccggaattg
     2461 caaatgccgt tcctgaaatc agcaataagt ggaaagaaaa acaataaatc cttacaagta
     2521 tttgtaagtc agataaataa caataagtaa accaactgga atatctgtaa aatatttccc
     2581 ggatgatttg gcaatagaga attcagtcta aaaaaccgca caatagtgcg attttgtcca
     2641 ttggagatat ttaccttacg gagcctttca ctttcgtatt ttccaggaca tctctgtaga
     2701 aatagaccat gacggccaca ttgacattga ctccgggggc agaaccgatc ttagtgccgt
     2761 tcttatccag gacagtccca tcggttttaa tcgtgccaac cggtttgctg ttcgcgtcaa
     2821 ccactttgcc atcctgcact atacgggcgt actcttttcc cagcctgtcg cggaaaatgc
     2881 cgttcttcac agaaccgatg accgctcttt tcttaatgat gtcgccggct gccgtgtact
     2941 ggaaaaatac ccggttgcgg gcatttctta cgtatcccat ctggtcgtgc gtgaagatca
     3001 gggttccggc agcatcgaac attttggtgg ccgttgcccc tttggtgttt gccgtaattg
     3061 ttcccggttt tttgtcggac gtactttcct tgttgaccgg tttattttta ttaacctgtg
     3121 ccaatactgg aaatgcaaaa aggattcccg acaggatcat acatattgta atcagctttt
     3181 tcatattaat ttggaatata tttttaacat taacattaaa aatccaaaat tagtataaaa
     3241 taagcttgtt ttcccaaatc agagcgatgg cccgcgcgag agatcactga aaggatcatc
     3301 gaacataatc acatcgtctt caacaaactc cagcattccg ttctccaggt cgatggtata
     3361 aatatagaag caggtatcgc attcacccgt tgaactcacg gcataaagca atccatcgct
     3421 gccaaaagct atctgtgcat cctcactgac cgctaccggc atgctgaaaa aggccggctc
     3481 cccggcatcc cagcgaagca actgcatgct gcccgattgg ttttgcgcca ggatatacag
     3541 gctttcgccg gaggaatcca tggctatatc attgactgcg tagccggccg gcagtgtatc
     3601 caggggcatt tcctccgcaa tcagccaggt actggtgaaa ctcacgcttt ggatcgatcc
     3661 ggtggaattg ttgatgtagt aatagtgatc cccttgaaac gtcccgcagg tggatggccc
     3721 tgacaggatg ccggcacaga atgaaggctc ttcaccctgc aggtggttga tccataattc
     3781 ggaagtggtg tatttgataa aaaacagcaa accggtcacc ggatcgtaat cggccccgtc
     3841 ataaaatccg gggctgccgc cgacgtattc ttccaggatc atttcaacac aacctgttga
     3901 gatatccagg tgatagatcc gtaaggagtc gttggcgcag gaggtgccat acagaaccgg
     3961 tccgggctcc tgcggcggat cgtagtaata gtcgtcgtac caaccccagc ctttgtcgct
     4021 gaaagtataa ctgccctcgg cccaggcact ctcggttttt cctccggggc catgaactac
     4081 acagtgagcg gccacgacaa accccggcga ggcgcacggg ggaagcgacg acagtggcac
     4141 gcgataagta aatgtgctta cccgcggact atggcatccc gagttgggga actgaccgat
     4201 cttgggcctg cccggcttat tcagcggcat tcctgctttg gttccggcat acatatgtgt
     4261 ttccgacatc ttccatcccg gggcgctgca atcataagtg gcatagaagt tggcattttc
     4321 atcaatcccg taagtgaccg tgccgacatt gatgttttta ccggcccaca atgtatggac
     4381 catctccccg gttttgagat attggaaata cccgttccat ttgtccggca tttcttcttc
     4441 cgtcatgatt tcagcacaac ccgacatgaa aactgccacc agcatcattt caattaaatt
     4501 cttcataaga aagtgtttta aggattaaaa ttcagtgaac tggggtggga tggaaaattt
     4561 aatccggcag atcggtcggg cagagggatg atcgctttct tatctattaa tccggttttt
     4621 gaccaaaaaa tacccggtgg agaggaaata tttttttaac ttattgaggg agagtagagt
     4681 aggcagtcgg cagtcgatag tcggcagtgg gtagttctgc gaattgattg gcgattgtta
     4741 taccgattct gaagcggttt gcgaattaac aaagttgttc tccccggtat aagtcattct
     4801 aagcagcttt gctgctgcaa acctgcaaag aatgagtata attagtaagc atgaagaaat
     4861 agtgattcta caacagaatc tcaatacctt cccttttcac gacctcaatt aaaggtgaaa
     4921 tgtcgtcctt agtaaaacgg gttgtgctga tcagataggg ttcaatccgt gtgtcgacat
     4981 cctttgtcaa tatccatgcc cgggattttt tctgaatatc attatcggaa atcagtccgg
     5041 ataccagcat aaggtcaata tcgctgtttt cggaattaga gccggaagca tagctgccat
     5101 aaaggaatgc tttgtcaatc ccaagacctt caatatcaag aagataaacg tatctcctta
     5161 taatatttat aactttttca tcagccattg gtgaaattct tttgtctgag tgaaaatttc
     5221 aagaaccaga aatatttcac tggtgatcaa atcttcgttt gaaccgt
//
LOCUS       DAOQXS010000163         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16046_length_5249_cov_4.045630, whole genome shotgun sequence.
ACCESSION   DAOQXS010000163 DAOQXS010000000
VERSION     DAOQXS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16046_length_5249_cov_4.045630"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKM34_05780"
     CDS             complement(<1..599)
                     /locus_tag="PKM34_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ83133.1"
                     /translation="MTETPGLQQFFFQQIREKIPDNMSFVHEIAELLEISYDSAYRRI
                     RGEKELSLEELVKLSKKFNLSLDALAGLDPNHVVFRKFALHPGDFNIKDWLLQIRDDI
                     RRIQTARNPEIIYAAKDPPVFHYFHFPEIAAFKFFIWEKTIFNFPEYENKLFRLDDVN
                     PEIVDIGNDILRAAIKVPTTEIWNEDTFRILMRQIEYYW"
     gene            complement(726..1676)
                     /locus_tag="PKM34_05785"
     CDS             complement(726..1676)
                     /locus_tag="PKM34_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNQ83134.1"
                     /translation="MIEKRIIAIIGATGAQGGGLADAILSDSQSEFSVRAITRNINAD
                     KAKALADRGAEVVSADLDDTESLEKAFNGAYGVFGVTNFWEHFSPEKETAQAKNIADA
                     ALAAGIKHVIWSSLEDTRDFIPLDDNRMPTLMGKYKVPHFDSKGEAEKFFQQPGLTST
                     ILRTSFYWDNYIYFGLGPKKGPDGVLAITFPMGDKKLPGIAAVDIGKCAYGIFKAGEK
                     YKNKTIGIAGEHLTGAQMAASFTRVIGAEVKYNDVPADVYRSFGFPGADEMGNMFQFK
                     RDFEADFVGGSNLEVARSINPSLQSFDQWLDQNAGKISIE"
     gene            1891..2706
                     /locus_tag="PKM34_05790"
     CDS             1891..2706
                     /locus_tag="PKM34_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83135.1"
                     /translation="MILRPRLKAEIERSLSVSPVVALLGPRQCGKTTISRLIAKNHKA
                     VIFDLEDPVDYQQLSAAPLITLQSLNGLVIIDKVQKMPELTGILRVLADRSNSPAKFL
                     ILGSASPVMMKNASESLAGRVSFIDMSGFNLEEIGAEHLRMLWTRGGFPRSFLGKDER
                     ISFQWRQDFIRTFLERDIPQLGISIPAVSLRRFWTMLAHYHGNIWNGSEFARSIGTTE
                     PTARRYLDILSGAFVVNQLQPWHENLKKRQVKSPKVYIRDSVLLLGHSRRGRN"
     gene            2939..>5249
                     /locus_tag="PKM34_05795"
     CDS             2939..>5249
                     /locus_tag="PKM34_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ83136.1"
                     /translation="MNAIRRFIKTVPKWAFVVVLVVIFVQTANMFYNARMPVSGEREN
                     NDNTNGYYIIDFVDPGGPVDKAGIKIGDTIVSCNHYPLEEWFSDDHGQRAGDTLIFGM
                     LRNGIEVGTPVIVTSRLSMSPWIYWPPYIFFILVSGLSLVILHLKPKDRSATLYFLYI
                     QGSAIMAIGGFYLLEDPLSIFMALTFLFCGMLTPAILIHFHLIFPKTSVLFNRFKYLP
                     KIFYGAAFLFFLFHTVCIFYQSFITSFYAPLPFDPILIGLRWAAFASILAIAIAIFQL
                     FTIKDTLSRNQLLIIVTGTIFGVIFTIFYAVFYDYINSLWGVYPNLVQFSMRISSIIL
                     VICLLIAIFRFRIWQMEIVLKKVFLYIIATAIIILSYLGLLYLVDFFTLEETKTTRFI
                     ALALSVLIFLILRDRIQRLIERMFHRENYDSTTVVSEFEEGLGGVYRIEDLGYTILVR
                     MDHIFHFNSAALYLNKEKQTYESGFAIGTTGQQPRIEFKISKAFENKLLKQRVFSPAE
                     LSEATVLPGLEQVDLVVPMIKDGTPFGFFLCGPKKSEKAFSMQDIRVLSLVAKRVIAL
                     FQTASLYQKDLDRQLMLERERARIAQDMHDDIGAGLTKIAMMSERAIGERGTVNSERM
                     EKVAATSREMITRLNVIVWALNPRYDNLESLISYARRYFGEYLENFGIVFRMEEPEEI
                     PEWPVNPDFRRNAFYAWQEAIHNAGKHGKCTEVLFEVRIDGRKLLVTITDNGKGFDKE
                     KHGSGGNGLLNMKKRAEELGGTFEITSEVG"
BASE COUNT         1350 a         1158 c         1229 g         1512 t
ORIGIN      
        1 atccagtaat attcgatctg ccgcatcagg attcggaaag tatcttcatt ccagatctcg
       61 gtggtaggga ctttaatagc tgccctgaga atatcgtttc cgatatctac tatttcgggg
      121 ttgacatcat ccaggcgaaa caatttattt tcatattccg gaaaattgaa tatcgttttc
      181 tcccagatga aaaatttaaa agcagcgatt tcgggaaaat ggaaataatg gaacaccgga
      241 ggatctttgg cggcatagat aatttcaggg ttcctggctg tctgaattct ccggatatca
      301 tcccttatct gcaggagcca gtctttgata ttaaagtcgc ccggatgcaa agcaaatttc
      361 cggaagacga cgtggttagg atcgaggccg gcgagagcat ccagcgagag attgaatttt
      421 ttactgagtt taacgagttc ttcgagcgac agttccttct cccctcttat ccttcggtaa
      481 gcactgtcgt aactgatttc cagcagttcg gcaatttcat ggacaaaaga catgttatcg
      541 gggatctttt ccctgatctg ttgaaaaaag aattgctgaa gacctggcgt ttcagtcata
      601 atgatgtaaa tataagtaat ttatatttac attgaatttt tacccggaaa aatgttttcg
      661 tagcccgtcg ggcttcagat ttcgtgctcc gaggggtaaa cccgtcggtt atggggatat
      721 gaagtttatt cgatggagat tttgccggcg ttttgatcga gccactgatc gaaactttgc
      781 agtgacgggt tgatagacct ggctacttca aggttgctgc cgccgacgaa atcggcttca
      841 aaatcgcgtt tgaactggaa catgttaccc atttcatcgg cgccggggaa gccgaaactg
      901 cggtagacat ctgccggcac atcgttatat ttcacttctg cacctatgac cctggtgaaa
      961 gaggctgcca tctgggcgcc ggtgaggtgc tcccctgcga ttccgatggt tttgtttttg
     1021 tatttttcgc ctgccttaaa aatcccataa gcacacttac cgatatcgac ggcggctatg
     1081 cccggaagtt ttttatcgcc catcggaaaa gtgatcgcca gtactccgtc cggtcctttc
     1141 tttgggccaa ggccgaaata aatataatta tcccagtaga aggaagttct gaggatagtc
     1201 gacgttaaac ccggttgttg aaagaacttt tccgcctctc cttttgagtc gaaatgaggc
     1261 actttgtatt ttcccatcag ggtcggcatc cggttgtcgt caagcgggat aaagtcacgg
     1321 gtatcttcca gcgacgacca gataacatgc tttatgcctg ctgccagagc cgcatcggca
     1381 atattctttg cctgtgctgt ttctttttcg ggtgaaaaat gttcccagaa attggtaacg
     1441 ccaaatacac catatgctcc gttaaaggcc ttttccaggc tttcagtatc gtcgagatca
     1501 gccgatacta cttccgcgcc cctgtcggcc agggctttag ctttatcagc attaatatta
     1561 cgggtaatag cccttaccga aaattcgctt tgtgaatcgg ataaaatggc atcggccagt
     1621 ccgccaccct gagcgccggt tgcaccgatt attgctatta ttcttttttc aatcattatt
     1681 tagttaatta aagttgacaa tcataaactc atagaaatgc attttgttca tttggctgac
     1741 gattgaaatg ggaaaatcgt gaagtactgg caaaccggca ttggttcctg gttcggggtt
     1801 ttttaaatta atatgtctga tttgttaaca atttaagtat atctgtcctt gtattttaaa
     1861 ataacgtctt ttaattttca aggataaatt atgattcttc gtccacgact caaggccgaa
     1921 attgaacgat ccttatcggt ttcaccggtg gttgcgcttc tcggtccgag acaatgcggc
     1981 aaaaccacga tcagcaggct tattgcaaag aatcataagg cagtcatatt tgacctggaa
     2041 gatcctgttg attaccagca attatctgcc gcacccctta tcacacttca atcgctaaat
     2101 gggcttgtca ttattgataa agtgcagaag atgcctgaat taactggcat cttaagggtt
     2161 ttagctgacc gcagcaactc tcctgcaaag tttctgatac tcggcagcgc ttcacctgtt
     2221 atgatgaaga atgcttctga atcgcttgcc ggcagggtgt cttttattga catgtcgggc
     2281 tttaacctgg aagaaatcgg ggcggaacac ctccggatgc tttggaccag gggtggattt
     2341 ccacgatcat tcctgggcaa agatgaacgg ataagcttcc aatggagaca ggactttatc
     2401 cgtacttttc tggagagaga tattccccag ttgggaattt ctatcccggc ggtttcattg
     2461 agaagattct ggacaatgct cgcccattat catggaaata tctggaacgg ttctgaattt
     2521 gcacgctcca tagggactac cgaaccaaca gccagaagat accttgatat tctttccggc
     2581 gcttttgtag ttaatcaatt acagccatgg catgaaaatc taaaaaaaag gcaggtaaaa
     2641 tcacccaaag tttacatcag ggactcagtt ttacttctgg gccactcacg ccgggggaga
     2701 aattgatctg ctttcaatac gcaacggcat taaaacagga tttgagatca attactctga
     2761 tgcaccgaag gtgaccaaat caatgctcct tgcaatcgaa gatctttccc cggaaaggat
     2821 attgattatt tacccgggtg agaaagatta tgcgctgcat gataaaattc aggtgaaggc
     2881 tgcgaagaat atctggattt cttccctacc tttacccgtg taaaccaacc tctctgcctt
     2941 gaacgccatc agacgattca taaaaaccgt tccgaaatgg gcatttgtgg ttgtgctggt
     3001 cgtgattttc gtgcaaacag ccaatatgtt ctacaatgcc cgcatgccgg tcagcggtga
     3061 aagagaaaac aacgacaaca ccaatggtta ttatatcatt gactttgtcg atcccggcgg
     3121 gccggtagat aaagcaggga tcaaaatcgg cgatacgatt gtttcctgca atcattaccc
     3181 gctggaggaa tggttttctg atgatcacgg acagagagcc ggcgacaccc ttatttttgg
     3241 aatgctgagg aacggcatag aagtggggac gcctgttatc gtcacatcca ggctgtcgat
     3301 gagtccctgg atatattggc ctccatatat atttttcatt ctggtcagtg gattaagcct
     3361 ggtcattcta catctgaaac cgaaggaccg gtcagctact ttgtatttcc tgtatatcca
     3421 gggatctgcc ataatggcca tcgggggttt ttatttgctg gaggaccctt tatccatatt
     3481 tatggcactg actttcctgt tttgtggcat gttaacaccg gctatcctta tccatttcca
     3541 cctgattttc ccgaaaacat ctgttctttt caatcgcttt aaatatttac cgaagatatt
     3601 ttatggagcc gcctttttat tctttctttt ccacacagtc tgcattttct accagagctt
     3661 tatcacttca ttttatgctc ctcttccgtt tgatcccata ttaataggcc ttcgctgggc
     3721 agctttcgct tcgattttag ccatcgccat tgccatattc cagctattca ccatcaaaga
     3781 taccctgtcc cgtaaccagt tattgataat agtgaccggt accatctttg gcgtgatatt
     3841 caccattttc tatgcggtat tttatgatta tataaacagt ctttggggtg tatatccaaa
     3901 ccttgtacaa ttcagcatga ggatttcaag cattatcctg gtcatttgcc tccttattgc
     3961 cattttccgt ttccgcatct ggcaaatgga aatcgtcctg aaaaaggtat ttctttatat
     4021 cattgcgacg gcaattatca ttttatccta cctgggccta ctttacctgg tcgatttctt
     4081 caccctggaa gaaacaaaaa caacacggtt tatagcgctt gccttatctg ttctcatttt
     4141 cctgatcctg agagaccgta ttcaaaggct tattgaacgg atgtttcaca gggaaaacta
     4201 tgattcgacg acagttgtat cggaattcga ggaagggctt gggggagtat atcggattga
     4261 ggatctggga tatacaatcc tggttcggat ggatcatatt ttccatttta attcggccgc
     4321 tttatacctg aataaagaaa agcagactta tgaatcaggt tttgcgattg gaactaccgg
     4381 ccagcagccc cgcatcgaat ttaagatcag caaagcgttt gagaataagt tattgaagca
     4441 aagggtgttc tcccctgctg agttatcaga ggccactgtc ctgccaggtc ttgaacaggt
     4501 ggacctggta gtcccgatga tcaaagatgg cacaccgttc gggtttttcc tttgcggccc
     4561 gaagaagtcg gaaaaagctt tttccatgca ggatatccgg gtgctttcgc tggtggcaaa
     4621 gagggtcatt gcgctgtttc aaacggccag cctttaccag aaagacctcg accgtcagct
     4681 catgctggag cgggaacgtg cacgcatcgc gcaggacatg cacgacgata tcggcgcggg
     4741 gctgacgaag attgcgatga tgagcgaaag ggccataggt gaacggggaa cggtgaacag
     4801 tgaacggatg gaaaaagtag ctgctacttc cagggagatg attacccggc tgaatgtgat
     4861 cgtgtgggcg ttgaatccaa ggtatgataa cctggagagc ctgatctcct atgcccggag
     4921 atacttcggg gaatatctcg agaatttcgg tattgttttc cggatggaag aacccgaaga
     4981 gatcccggag tggcccgtaa accctgattt caggcggaat gctttctatg cctggcagga
     5041 ggcgatccat aatgccggaa agcatggaaa atgtactgag gttctatttg aggtcaggat
     5101 agacggccgg aaactgttag tcacgatcac cgacaatggg aaaggatttg acaaagaaaa
     5161 gcatggatca ggcggcaacg gcttgctgaa catgaagaag cgggcggaag agctgggcgg
     5221 gacgtttgag attaccagtg aggttggca
//
LOCUS       DAOQXS010000164         5244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16062_length_5244_cov_3.071690, whole genome shotgun sequence.
ACCESSION   DAOQXS010000164 DAOQXS010000000
VERSION     DAOQXS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16062_length_5244_cov_3.071690"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="PKM34_05800"
     CDS             complement(<1..2148)
                     /locus_tag="PKM34_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83137.1"
                     /translation="MKKIILLFCSIFSLSLTAQVTYFENFDVAGSWTGVYNAYGVKTY
                     TNSADPVNDMFSSNLAWRETDYYTSSPYAWRIQQGSNYYFRYECQETVQAFQVKMARW
                     DNSPVPNVTIRYSVNSGSTYTTITTITGSYFSADKVYVLYSYTFPAPISPDPTLKVYI
                     EFLTTTGERMLYDDFQLTYLSALPLNPTSFDAIPASTTQNNILWALNTSSDSVMVAYN
                     AINTFGNPSGVYSIGQEISGGGTVLYKGTGTSTSHSGLNPNTEYYYKAWSKGASGLYS
                     TGVTDSSTTYKIQPSEYPALFAVADTGITITATWTDAGGTQPPDGYLIRISSQDNVTT
                     PVDGTPVTNDLDLSDGTGAVSVAQGVGKYTFYRLQGTTTYYLKIFPYTNSGSGIDFLT
                     DDPAPAGNASTQSIVNTNDFESGLFGDWDTISLAVKSRNWSIYTTNGAYGTSKSSGIN
                     GYQTPTDTLENDWLISPSLNLDAFADEYMVFFTWWRYGTDPNELKLKYSTDYVGGDPS
                     LATWAELSFAKPAVEQTWAPSGFIDLKGISGTNVHLAFHYLSVTGPRWWLVDEIEITG
                     DGISNPADFSAQTASMTEISLSWTKNTSLNDVLVAFNTIDEFGIPEGQLDAGAEIPGG
                     GTVLYHGQDQSFLHTELTPATTYFYKAWSVDPDNNYSPGVAGSATTQYPEPSGNPTGL
                     TATANGFSNITVTWTDSDASGYLVKASSTGFDAI"
     gene            complement(2151..3260)
                     /locus_tag="PKM34_05805"
     CDS             complement(2151..3260)
                     /locus_tag="PKM34_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNQ83138.1"
                     /translation="MFAVKAICLTFILATTGYLSSGQIPEGYYSTASGLSGSALKAAL
                     HDIIDDHTELSYTATVNALKVTDEDPNNSSNVILIYTGWSYPKSSYSYDWNREHVWAK
                     SHGDFGDYPPEGTDLHHLRPCDADVNSTKSNRDFSKGTSPYFLNGIPTGCYTAPYIWE
                     PRDEDKGDVARMMFYMAVRYEGDNGETNLELVSYVNSSPDNEPLYGNLDTLFKWHNND
                     PVSALEISRNNKIYSQYQGNRNPFIDHPEYVDLIWGDGIADEGEPVSHASDFTASNII
                     LNWNEPTTGVLPDAYLIGISTSSFEAIPVPADGVPVENSSISKNIAYGIGQYTFKNAI
                     PGTTYYFKIYPYKGFGEAINYKTDGNIQQVTIEIN"
     gene            complement(3430..3888)
                     /locus_tag="PKM34_05810"
     CDS             complement(3430..3888)
                     /locus_tag="PKM34_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83139.1"
                     /translation="MKAFKSDILPKQGFGEIMFGETSEKVIKLLGQPEDVENIEDVDG
                     FNTVVLYYYEKGITIFFEGKEKSVVACVETENPEATMYGKRIFEMTEKDIVALMKEKG
                     FEVAETEMETTGERRVSYDDAMIDFFFLDGDLVVVNWGVLVNEKGEIEEI"
     gene            complement(3894..4700)
                     /locus_tag="PKM34_05815"
     CDS             complement(3894..4700)
                     /locus_tag="PKM34_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83140.1"
                     /translation="MKDKVIIVTGASSGIGKALAAELSRRGAIVVLAARSADKLSELE
                     KELGGKGSKVLGVVTDVSKEEDCRKLVSKTVEKFGKIDVLINNAGISMRAIFEEVELD
                     VIRRLMDINFWGTVYCTKYALPYLLKTKGSVVGVSSIAGFKGLPGRTGYSSSKFAMQG
                     FLETLRIENLKKGLHVMIACPGFTSSNIRNTALAADGSMQGESPRDEAGMMSPEEVAR
                     RIAKGIENRRKLLVMTSQGKMTVILNKFFPYFIDKMVYNHMAKEPDSPFK"
     gene            4908..4981
                     /locus_tag="PKM34_05820"
     tRNA            4908..4981
                     /locus_tag="PKM34_05820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4942..4944,aa:Met,seq:cat)
BASE COUNT         1330 a         1189 c         1211 g         1514 t
ORIGIN      
        1 gattgcatca aatccggtcg atgatgcctt taccaggtag ccggatgcgt cggaatcagt
       61 ccaggtgacg gtgatattcg agaatccatt cgccgtagct gtcaagccgg tcggattacc
      121 cgagggttca gggtactgtg tggtagcgct gcctgccacg ccgggagaat aattattgtc
      181 cggatcaacc gaccaggctt tgtaaaagta ggtggtggcc ggggtcagct cagtatggag
      241 gaatgattga tcctgaccgt gataaagcac cgttccgcct ccgggaattt cggccccggc
      301 atcgagttgc ccctccggta ttccgaattc atcgatggtg ttgaatgcga ctaaaacatc
      361 gttaaggctt gtatttttag tccaactcag gctgatctca gtcatcgaag cggtttgcgc
      421 actaaagtcg gccggattgg agatgccatc tccggtgatc tcaatttcat cgaccagcca
      481 ccagcgcggt cctgtaacag aaagataatg gaatgccagg tgaacattgg taccggagat
      541 ccctttcaga tcgataaaac cggaaggggc ccaggtttgc tcaacagcag gtttggcgaa
      601 tgaaagttca gcccaggtgg ctaaagaggg atcaccaccg acgtaatctg tggaatattt
      661 cagctttaac tcattgggat cagttccgta gcgccaccag gtgaagaaga ccatgtattc
      721 atcggcaaag gcatcaaggt taaggcttgg tgagattagc cagtcattct caagggtgtc
      781 agtcggagtc tgatagccat tgatgccgct tgacttggaa gtgccataag ccccgtttgt
      841 cgtgtatatc gaccagttcc tcgatttgac ggcgagactg attgtgtccc agtcgccgaa
      901 taacccggat tcaaagtcgt tggtgttgac gattgactgt gtagatgcat tgcccgctgg
      961 ggccggatcg tcggtcagga agtcgatacc tgaacccgaa ttggtatatg ggaaaatttt
     1021 caaataatag gtagttgttc cctgaagccg gtaaaacgtg tattttccaa caccctgtgc
     1081 aactgaaaca gcccctgttc catccgaaag atcaagatca tttgttaccg gagttccatc
     1141 gactggcgtt gttacattat cctggctgga aattctgatc aggtatccat ccggtggctg
     1201 cgtcccaccg gcatcggtcc atgtggctgt gatcgttatc cctgtgtcgg caacggcaaa
     1261 gagcgccggg tactcggaag gctgtatttt gtaagtagtt gaactgtccg tcactcctgt
     1321 ggaatacaat ccgctggcac cttttgacca ggctttgtaa taatattcgg tgtttggatt
     1381 taatcccgaa tggcttgttg atgttccggt gcctttatac agcactgtac ccccgcccga
     1441 aatttcctgg ccgattgaat aaacgccgga tggatttccg aatgtgttta tcgcattata
     1501 ggcaaccata acggaatcgc tactcgtatt gagggcccat aaaatattgt tttgcgtggt
     1561 ggaggcggga attgcgtcga aagatgtcgg attaagaggt aaagctgaga gatatgttaa
     1621 ctgaaaatcg tcgtaaagca tccgttcgcc tgtggtcgtc aagaattcaa tatagacctt
     1681 cagagttgga tctggagaaa tgggagcagg aaatgtatag gaatacaaaa catagacctt
     1741 atctgccgaa aaataactgc ccgttatggt ggtaattgtg gtgtatgttg atccggaatt
     1801 tacactatat ctgatggtta catttgggac aggtgagtta tcccatcttg ccatctttac
     1861 ctggaatgcc tggactgttt cctggcattc atatcggaaa tagtaattgc tgccctgttg
     1921 aattctccag gcataaggtg aacttgtata ataatctgtt tcacgccatg ccaaattgct
     1981 tgaaaacatg tcgtttaccg gatcagcact attagtatat gttttaacac cataggcatt
     2041 atacacccca gtccatgatc cggctacatc aaaattctca aaataggtta cttgtgcagt
     2101 cagcgaaaga ctaaatatcg aacaaaatag taaaataatt tttttcatat tcaatttatt
     2161 tctatggtta cttgttgaat gtttccgtcg gttttataat tgatggcctc tccgaagcct
     2221 ttgtaaggat atatcttaaa ataataagtc gttcccggaa tggcattttt aaaggtgtac
     2281 tgaccgattc catatgcaat atttttactg atggaactgt tctccaccgg gacgccatcc
     2341 gccggaacag gaatagcctc aaaactgctg gtgctgatgc cgatcaggta agcatcgggc
     2401 aatacccccg tcgtgggctc gttccagttc agaatgatgt tcgaggccgt gaaatcagag
     2461 gcatgcgata caggctcccc ttcatcggca atgccatcac cccatatcag atcaacatat
     2521 tccggatgat cgatgaatgg attacggttg ccctggtatt ggctgtagat cttgttgtta
     2581 cgggatatct ccaaggcgct caccgggtcg ttgttgtgcc atttaaacaa cgtatccagg
     2641 ttcccgtaca atggttcgtt gtcaggggag gagttgacat aactgaccaa ttccaggttg
     2701 gtttccccgt tgtcgccttc gtaccttacc gccatataaa acatcatccg ggccacatca
     2761 cccttgtcct catctctcgg ttcccagata taaggagcgg tatagcatcc tgtaggaata
     2821 ccatttaaaa aataaggtga ggtccctttt gaaaaatcac ggttgctctt tgtggaattg
     2881 acgtctgcat cgcagggcct gagatgatgc aggtcggttc cttccggagg atagtccccg
     2941 aaatcgccat gcgatttagc ccacacatgt tcccggttcc aatcatagga ataagaagat
     3001 ttcggatacg accatccggt ataaattagt atgacattgc tgctattgtt cggatcctca
     3061 tccgtcactt ttaatgcatt aacggttgcc gtataactca attcggtgtg atcgtcaata
     3121 atatcatgca gtgcggcttt taatgcactc ccgctcagcc ctgatgcggt cgagtaatat
     3181 ccttcgggaa tttgacccga agataaatac ccggtagtgg ccagaatgaa ggtcaggcat
     3241 atggctttta ctgcaaacat cagtaaatca tggtgttata aagcggctaa aatgtcctga
     3301 attttcagga gtgcaaagtt actaacagtt gcaggacatt tttgtgatgt tgataaatat
     3361 aaagaaaagt taatgtttga aacgatatgg acctattaat aaaacgaatg tattgttaca
     3421 aacgatatat taaatctctt cgatttcccc tttttcgtta acgagaactc cccagtttac
     3481 gacaaccagg tcgccgtcga ggaagaaaaa gtcgatcatg gcatcgtcat acgataccct
     3541 gcgttcaccc gtcgtttcca tctctgtttc ggccacctcg aaacctttct ctttcatcag
     3601 cgcgacaatg tctttttcgg tcatttcaaa gatgcgcttg ccatacattg tggcttccgg
     3661 gttctcggtt tcgacgcagg ccaccaccga cttttccttg ccttcgaaga aaattgtgat
     3721 acctttttca taataataaa ggaccacagt attgaacccg tcgacatctt cgatattttc
     3781 gacatcctcg ggctgaccga gcagtttgat gaccttttcc gatgtctctc cgaacatgat
     3841 ttccccgaag ccctgtttgg ggagaatgtc tgatttaaat gctttcattt tatttattta
     3901 aaaggtgaat ccggttcttt ggccatatgg ttatagacca ttttatcgat aaagtaaggg
     3961 aaaaatttgt taagaatgac ggtcatttta ccttgagagg tcatgaccag caactttctc
     4021 ctgttttcaa ttcctttggc gatccttcgg gcaacttcct cgggcgacat cataccggct
     4081 tcatcccggg gtgattcccc ctgcatcgag ccatcggcgg ctaaagcggt gttcctgata
     4141 ttggaagagg taaatccagg gcaggcgatc ataacatgca gtcccttctt caggttctca
     4201 atgcggagcg tctccagaaa tccctgcatg gcaaattttg atgaggaata accggttctg
     4261 cccggcaaac ccttaaaacc ggctatggaa gaaacaccca cgacggatcc ttttgttttc
     4321 aaaagatagg ggagggcata ttttgtgcag taaaccgttc cccagaagtt gatgtccatc
     4381 agcctgcgga ttacatccag ttcaacctct tcaaagattg cccgcatgga gatcccggca
     4441 ttattgatca aaacatcgat ctttccgaat ttttccaccg ttttgctgac caacttccgg
     4501 caatcctcct ctttgctcac atcggtaaca acaccaagta ccttgctgcc ttttccacca
     4561 agttcttttt ccagctccga cagcttatcg gcagagcgcg ctgccagtac aacgatcgct
     4621 ccacggcggc ttagctctgc agccagggct ttcccgatac ccgatgaagc tcccgtaacg
     4681 attataactt tatctttcat tacatacaat gttaggatat ttttcaatag ttggaggata
     4741 ttttttgtta agaatttaaa aaatcctggc ttatgagaat taaagataaa aaagtgctaa
     4801 ttttgcccgc tcaaaaatgg cgaggtagct caggtggtta gagcgtcgga ttcatatccg
     4861 ccggctggcg gacacgagtt caattctcgc aaagttattt gtttaatggc gaggtagctc
     4921 aggtggttag agcgtcggat tcataacccg gaggtcgagg gttcaattcc ctctctcgct
     4981 acattgaaag tcaggtattt acctgacttt tttattttct gtctatttat gaggtaaggt
     5041 gacgaggtaa ggaaaatttt tgaaagtttc tttattcatt ctcatctttc tttgggaaga
     5101 tctttagccc agccgtcttt ttaacggctt ccatctgggc gagataatgt ttccttacga
     5161 cttcatcatt actatgatcg cttacaagcg tggtgagtgt aataccaaat tgagcttcag
     5221 attggttcat ataggtcttt ctta
//
LOCUS       DAOQXS010000165         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16098_length_5234_cov_3.642788, whole genome shotgun sequence.
ACCESSION   DAOQXS010000165 DAOQXS010000000
VERSION     DAOQXS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_5234_cov_3.642788"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PKM34_05825"
     CDS             <1..1618
                     /locus_tag="PKM34_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNQ83141.1"
                     /translation="ISSAPIYRENFLYFALPAIGILLAIFFVKYVIKDHVEHGVPSIL
                     YAISRSNGIIRRHNMFSSIVTSALTVGFGGSVGLEGPTLATGAAVGSSLGQYLRLSYK
                     DLILMMTLACAGAMAAIFKSPIAAIVFAVEVLMIDLTMASLIPLLIASVTALLTSYFF
                     LGNAVLYPFEVTEAFYYKQIPYYLILGVFTGFISVYFTRMYIRIEKYFKRISSWWLRW
                     ISGGMILGMLIFFFPALYGEGYEAVNTCMKGDYSPLFNGSPFYEFQDSIWVVFLLFIL
                     VLFFKVVATATTFGAGGVGGIFAPSLFMGVIAGLFFAEMVNYSGLTLLSGSNFAMVGM
                     SGVIAGVLHAPLTGIFLIAEITGGYDLIIPLMLVSTISYATTRMFVSNSVYTYQLAAR
                     GELITHDKDKTAMSLLRVNDLIETDFRTIGPDARLRNLIQVISESARNIYPVVDENNR
                     FFGFVRLDDVRHIMFKQELYDQVIVKDIMVRPDTFVSPDDSMETVVEKFAHYDKYNLP
                     VLLNGIYIGFVSRANVFRNYREIVRKFSED"
     gene            complement(1677..3725)
                     /locus_tag="PKM34_05830"
     CDS             complement(1677..3725)
                     /locus_tag="PKM34_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ83142.1"
                     /translation="MMPDHRFTNQLIRESSPYLLQHAHNPVEWHPWNEQTLSKARSLD
                     KMLLISIGYSSCHWCHVMERESFEDVKVAEIMNRNFICIKVDREERPDVDQVYMNAVQ
                     LITGSGGWPLNCFTLPDGRPFFGGTYFRKNQWVQLLENISTLYLKRRKEIEDQADALT
                     EGIRKASLIKPAESVRAITREDITRITNSLTGQMDELEGGFGGAPKFPMPAALAFLLT
                     TGEITANEKVEKLVKLTLRKMSYGGIYDQAGGGFARYSTDSQWKVPHFEKMLYDNAQL
                     ASLYLEASQVYGSKEFRETAEETLGFVMREMTSPEGGFYSALDADSEGKEGRFYTWTA
                     GELKILTGNQGDLVRKYYHTGGKALWEEGQNILVRTQSPDEFARAQGLQPSAFKAILK
                     SAKAKLLKERNRRTMPGLDNKILASWNGLMMKAFADAGRITGQKKYLDAAVKTAGFIH
                     ANMTGPDGHIFRCFNKGKSYINGFLEDYCFVAEGLLALYLATFREQYLYRALELIETA
                     IRDFYDSESSYFFGSSASDAPLVARQQEIYDSVIPSSNSVMAGLLYKLGVIFEKDDFI
                     EKQAKMVSGVREKALQFPSAFANWASVMLQTALPYYFVVITGDDYPEKIATIRKHFRS
                     QIFFCAARERSELPVFKDRFTENQTMIYICTGRECLLPTTSVDEALQQLAGKIRLTPE
                     "
     gene            complement(3774..4811)
                     /locus_tag="PKM34_05835"
     CDS             complement(3774..4811)
                     /locus_tag="PKM34_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ83143.1"
                     /translation="MSGNLVTSEIIEQVARKAGITKKLASELLHAIPEIIAEGLQRDG
                     EVRVRGLGTFRMKWTQTRIGRNPKTGEQVPIPPHNRLVFLPEQAFKDFINRDLVALEY
                     QVIPEDASVPEPEKPAVEIPEPIIISPSWPEPLPEPEPYAIAGTGIDQEPGSTGSRRR
                     LHWIIPAAIAVIAILTVVFYFRIFREKEPVAVGSQQSAVGSQQPAVGSQQSADSSRQP
                     AVGSQQVEESNNDSTLIIKNSELSIKNSTLITSGVHLFQVSRDVYEGNPYLWALIYKA
                     NREIITNPQNLPSGLTLIIPGLEGSPNKLSRRDSSEVSEGYRLLSEYYREKDPSLSDG
                     FLKTSSQFRPE"
     gene            complement(4808..5095)
                     /locus_tag="PKM34_05840"
     CDS             complement(4808..5095)
                     /locus_tag="PKM34_05840"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ83144.1"
                     /translation="MNTKEFIQVLAEKLAITQKEASGLLEHTTAVMREAVAADQKVTI
                     THLGSFQLKKTASRAAYLPALDKKAIVPPKNSVQFHAADSLKDNLKKSPEK"
BASE COUNT         1124 a         1341 c         1335 g         1434 t
ORIGIN      
        1 catcagcagc gcgccaattt accgcgaaaa tttcctctat tttgctttgc cggcgatagg
       61 gatccttctg gccatctttt tcgtaaaata tgtcattaaa gatcatgtcg agcatggcgt
      121 tcccagcatt ctttatgcca tcagccgttc caatggcatc atccggcgcc acaacatgtt
      181 ctcctccatc gtcaccagcg cactgaccgt cggtttcggc gggtcggtgg gattggaagg
      241 cccgacactg gctaccggcg ctgcagtagg ctccagcctg ggccagtacc tccggcttag
      301 ttacaaagac ctgatcctga tgatgacgct ggcctgcgcc ggcgctatgg cagccatctt
      361 taagtcgcct attgcggcca tcgtctttgc cgtggaagtc ctgatgatcg acctgaccat
      421 ggcttccctc atccctctgc tgatcgcttc agtcaccgcc ctgcttactt catacttttt
      481 tcttggcaat gccgttcttt accctttcga agttaccgag gccttttatt acaaacaaat
      541 accctattat ttaatcctgg gtgtctttac cggttttata tcggtctatt ttacccggat
      601 gtatatccgt attgagaaat attttaaaag gatcagcagc tggtggctga gatggatatc
      661 gggcggcatg atcctcggga tgctgatctt tttcttcccg gcactatacg gtgaaggcta
      721 tgaagctgta aacacctgta tgaagggtga ttacagtcca ttatttaacg gaagcccttt
      781 ttatgagttc caggacagca tctgggtagt gtttcttctc tttatcctgg ttctcttttt
      841 taaggtggtt gcaactgcca ctacattcgg cgcgggggga gtcgggggga tattcgcacc
      901 gtcgctgttc atgggggtta tagcagggct tttctttgcc gagatggtca attattcagg
      961 cctgactctc ctgtcgggtt ccaatttcgc catggtaggg atgagcggag tgattgccgg
     1021 cgtgctccac gcgccgctga cgggcatctt cctgatcgcg gagatcactg ggggctacga
     1081 cctgatcatc ccgctgatgc tggtctctac catcagctat gctaccaccc ggatgttcgt
     1141 ctccaattcg gtctatacct accagctcgc tgccagggga gagctgatca cccatgataa
     1201 agacaagaca gccatgtcgc tgctccgggt caacgacctg atcgaaaccg acttccggac
     1261 catcggaccg gatgcccgcc tccgcaacct gatccaggtt atcagcgaat ccgccaggaa
     1321 catctacccg gtagtcgatg aaaacaacag gtttttcgga ttcgtcaggc tggacgatgt
     1381 ccgtcatatc atgttcaaac aagaactcta tgaccaggtt attgtcaaag atattatggt
     1441 caggccggat acttttgtct cacccgatga ttccatggaa acggtcgtgg agaaatttgc
     1501 gcattacgat aaatacaacc tgccggtgct tttaaatggc atttatatcg gttttgtctc
     1561 ccgtgccaat gttttccgga attaccggga aatcgtcagg aaattttccg aagattgaag
     1621 ccgtcagttc cctccttagt cgggatgacg cattctctaa gaaaataaag ggagcgttat
     1681 tccggcgtta gccgaatttt acccgctaac tgctgcagtg cttcatcaac cgatgttgtt
     1741 ggcaggaggc actcacggcc ggtgcatata tagatcattg tctgattttc agtgaacctg
     1801 tctttgaaaa ccggaagttc actcctttcc cttgctgcgc aaaaaaatat ttgagaccgg
     1861 aaatgctttc ggatagtcgc gatcttttcg ggataatcat cgccggttat gacaacgaaa
     1921 taatatggaa gggccgtctg tagcattacc gatgcccagt tggcaaaggc ggaagggaat
     1981 tgcagggctt tttccctgac acccgaaacc atttttgcct gtttttcaat aaaatcatct
     2041 ttttcaaaaa taacgcccag cttataaagc aaacccgcca tcacgctgtt ggaggagggg
     2101 atcacggagt cgtaaatttc ctgctggcga gctaccagcg gagcatcaga agcggatgat
     2161 ccgaaaaaat aactgctttc cgaatcgtag aaatcccgga tcgccgtttc aatcaattcc
     2221 agagcccggt acaaatattg ttccctgaag gtggccaggt aaagggccag gagcccttcc
     2281 gccacaaaac agtaatcttc caggaatccg ttgatataag atttaccttt attgaaacag
     2341 cgaaaaatat gaccatcggg gccagtcata tttgcatgaa taaacccggc agttttaacc
     2401 gccgcatcca ggtatttttt ctgtccggta attcgtccgg catcggcgaa tgccttcatc
     2461 atcagtccgt tccaggaggc cagaatcttg ttgtcaagcc ctggcattgt ccttctattc
     2521 cgttctttaa gcagttttgc tttggcgctc ttcaggatgg ctttaaatgc cgatggctgc
     2581 aaaccctggg ccctggcaaa ttcatcgggt gactgggtgc gcaccaggat attttgcccc
     2641 tcttcccaca aagctttacc tccggtatga taatattttc gcaccaggtc gccctggtta
     2701 ccggtgagaa tcttaagctc accggctgtc caggtataga accggccttc tttgccttcc
     2761 gaatcggcat ctaaagccga ataaaagccg ccttcgggcg aggtcatttc cctcatgaca
     2821 aatcccagtg tctcctcggc cgtttcccgg aattcctttg atccatatac ctgtgaggct
     2881 tccaggtaga gagatgcaag ctgtgcattg tcgtacagca tcttctcgaa atgggggacc
     2941 ttccattggg aatcggtcga ataccgggca aatccgcctc cggcctggtc atagattccg
     3001 ccgtatgaca tcttccgtaa ggtcagcttc accagttttt cgaccttctc attcgcagtt
     3061 atttcgcctg tggttagtaa gaacgccaag gcagctggca tcggaaattt cggggcgccg
     3121 ccgaatcccc cctccagttc gtccatctgg ccggtcaggc tgttggtgat ccgggtaatg
     3181 tcttccctgg taatcgccct gaccgactct gccggtttta tcaaggaggc cttcctgatc
     3241 ccttcggtga gtgcatcggc ttgatcctca atctcttttc ttcttttaag atagagtgtc
     3301 gaaatattct ccaggagctg gacccattgg ttttttcgaa aataggtgcc gccgaaaaag
     3361 ggacggccgt cgggcagggt aaagcagttc agcggccagc ccccgctgcc cgtaataagc
     3421 tgcacggcgt tcatgtacac ctggtcgacg tcgggccgct cttcccggtc gaccttgatg
     3481 cagatgaaat tgcggttcat gatctccgcc actttcacat cttcgaacga ttctcgctcc
     3541 ataacgtgac accagtggca ggatgaatag ccaatgctga tcagcagcat cttgtcgagc
     3601 gaccgggctt ttgaaagagt ctgctcattc cagggatgcc attccacggg attatgcgcg
     3661 tgctgcagca ggtaggggct gctttcacgg ataagctggt tggtaaaccg gtgatcgggc
     3721 atcatgagat aaagttacga ttttggccgg cggtattcag gcagttgata actttattcc
     3781 ggccggaatt gtgaagaggt tttcaggaac ccgtccgaca aggagggatc tttttcccgg
     3841 taatattctg atagtaagcg atagccttcc gacacctctg aggaatcgcg gcggctcagt
     3901 ttgttgggtg agccttcaag gccgggtatg atgagagtca gaccggaggg gaggttctgc
     3961 gggtttgtga tgatctccct gttggccttg tagatcagcg cccagaggta cgggttgccc
     4021 tcgtaaacat cccgggatac ctggaaaaga tgaaccccgg aggttattaa tgttgaattc
     4081 ttaatgctta attctgaatt cttaatgatt aatgttgagt cgttattgga ttcctctacc
     4141 tgctgactgc cgactgccgg ctgccgactg ctgtctgccg actgctgact gccgactgcc
     4201 ggctgctgac tgccgactgc cgactgctga ctgccgactg ctaccggctc cttctcccga
     4261 aagatcctga aatagaaaac gaccgtcagg atggcgatca cggcgatggc tgccggtatt
     4321 atccagtgca accggcggcg gctgcccgtt gacccgggtt cctggtcgat tcctgttccg
     4381 gcaatagcat agggttcagg ttcgggtagc ggctcaggcc acgatggact gatgatgatc
     4441 ggctccggaa tttccacggc cggtttctcc ggttcgggga ctgaagcatc ttccgggatt
     4501 acctggtatt ccagggctac aagatccctg ttgataaaat ctttaaatgc ctgttccggc
     4561 agaaacacca gccggttatg cggcgggata ggaacctgct cgcccgtttt cgggtttcgg
     4621 ccgatccggg tttgtgtcca tttcatgcgg aaagtgccca aaccgcgcac ccttacctct
     4681 ccgtcgcgtt gcaaaccttc ggctatgatt tcggggatgg catgaagcag ttccgaagcc
     4741 agttttttgg tgatccctgc tttgcgtgcc acctgttcga tgatttcgct ggttacgagg
     4801 ttgccgctca tttctccggg gattttttga gattatcttt cagcgaatcg gcggcatgga
     4861 actgcacgct gttttttgga gggacgatgg cttttttatc gagcgctgga aggtaggcgg
     4921 cgcggctggc tgtttttttc agttgaaagc ttcccaggtg ggtgatcgtc actttctgat
     4981 cagcggccac agcttcgcgc attaccgcag tggtgtgttc aagcagtccg gaagcttctt
     5041 tctgtgtgat cgcgagcttc tccgccagca cctgtatgaa ttccttggtg ttcagggttg
     5101 atttctttgg attgtttcaa agtgtaatat tacgaaaaaa gattaggttt tgcaagtttg
     5161 agatggagtc tcctgaaaaa cgagactcca tctgggctaa tcgagatgga gtctcaaaat
     5221 caatctttat attg
//
LOCUS       DAOQXS010000166         5221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16144_length_5221_cov_3.157762, whole genome shotgun sequence.
ACCESSION   DAOQXS010000166 DAOQXS010000000
VERSION     DAOQXS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16144_length_5221_cov_3.157762"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..2768
                     /locus_tag="PKM34_05845"
     CDS             300..2768
                     /locus_tag="PKM34_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ83145.1"
                     /translation="MINTQFPEKPALILRDHTDTYQQLIQRIREYAALFAGKNIEKVA
                     IFSENRPEWIYAFYSAWYNSALVVPIDFLSSAADAAYILNDCRPELLFAGKSVSATLA
                     KMYGKLEFKPEIIYFEDVRLTGDTIYSEWDFHPIEDKTAAIIYTSGTTGSPKGVMLSF
                     KNISANIKAVTEDYPIFTSDRPVLMLLPLHHIFPLAGSMVAPLKAGGCVVMSPSMQSS
                     DIIETLKAHPVRIMIGVPRLYELLYKGIKAKIDAKAIGRLFFRIVKLSRSKRLAKKIF
                     KKVHGGLGGELEIMVSGGAALNKTVGQFFDTLGFDVMEGFGMTEASPMITFPRPGKVV
                     IGTAGQALPGLKVEIREGEVVAKGPNIMQGYYKRPEETAEVLRDGWLYTGDLGEIDSK
                     GYLRITGRKKEIIVLPNGKNVNPVELEQKLEKEYACIKEAGVLVQNDILHAVILPDFN
                     VLAKDGVKDADLYFRTDVFPAFNEELTSYKRIMQYTLVKEELPRTRLGKIQRFKLDEL
                     AVGKTKEKRQSAEEDSSEEYLAVKTFLESQVDMTISPEDHLEFDIALDSLGKLSLIDY
                     IEKTFGVKIEEEHLPKFPQVKSLVEYIKINKKWQKEDVTSWASALKEKVQVKLPRTWP
                     TLPMIKNSAKGFFAVYSRLKSRGTENIPEGPCIIAPNHESFFDGLYLVAALKMKTMKK
                     SYFYAKKTHVNNWFLRFMANRNNVIVMDVNKDLKDSIQKLAEVLRKGKNVIIFPEGTR
                     TKTGKMGDFKKTFAILSKELNIPVVPVAISGAYKAMPYRAIFPRPLSKISICFAEPVY
                     PDSFSSDELTAIVRDRISNMLNCR"
     gene            complement(2769..4220)
                     /locus_tag="PKM34_05850"
     CDS             complement(2769..4220)
                     /locus_tag="PKM34_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNQ83146.1"
                     /translation="MATERHFKKSLNLFDSAAITMGSMIGSGIFIVSADIARNVGSPG
                     WLLVVWAVTTLMTVLAALSYGELASMLPHVGGIYVYLKEAYSPLFGFLYGWTFFLVIQ
                     CGTIAAVAMAFAKFTGVLLPVISEQNILLDLGLFKFNTTQLLAIMMILFLTWINTRGI
                     KEGKRVQNIFTYSKVFILLAFIVIGIFVAKATQLNVFHSPGFWEASKIVDNQQVPVSG
                     FSLIIAIGLAMVGSIFAADAWYNITYTSDEVINPKKTIARSLFLGTLTVCLIYFLVNV
                     VYIIALPVRGIPDGATVMERGIQFATADRVATAAIYGIFGKNTELLMAVVVVISTFGC
                     NNGIILSGARVTYAMAKDRLFFKKTGELSKTGVPAFGLWIQAIWSTLLCLTGTYSQLL
                     DYVVFAALLFYILVIFAVFMLRIKHPEWERPYKAFGFPVFPIIYIAACLLIIIILLIY
                     KPLFTWPGLGIVISGIPVYYLWKRKSHKGPVTI"
     gene            4331..>5221
                     /locus_tag="PKM34_05855"
     CDS             4331..>5221
                     /locus_tag="PKM34_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ83147.1"
                     /translation="MSPFQPCIILDFNFVPLLFVVAIAWLIPMLLTILKADRVPSVLI
                     EIIAGYAAGHFLMQYVDADSMLVLNFLALTGLIFIMFLSGLEIDINSILASFPRKLSL
                     GTLSKNPILAGISHYLICLAISLAGTFLLSFYVEIPNVWFFSLIPTTTFMGIVYPVLK
                     NRGETKTYYGQTIITTSAIADIFSITLVTISALYLKFGFDYELFLVIAFFIFFVILYY
                     LGKKFSPGIFKRITFQKAHAATQLSMRGALALVFLFVVIAQIVGGEVVILGAFLSGLL
                     MSFFMSKERSILIIKLEGMGF"
BASE COUNT         1515 a         1098 c         1215 g         1393 t
ORIGIN      
        1 ggccgatcct ggatccccag gcttctttgt tagtgctcat tcgtcctggt ttagttaatt
       61 aattaaagga ttcaaagaaa atcgcgatta aaggtatggg aatttgccaa aataccaaat
      121 cccgatcctt aataaattac atgtatttat aatcaaaaat attgacttaa acgtgtaata
      181 cagttataca ctgacatttg aaaggaaata ttacccggga tttgtatcct gatatttaga
      241 ttccgggata tgaaattaat actaaatttg tattaatctt tcacttaccc aacccccaga
      301 tgataaacac tcaattccct gaaaagccgg ctctgatcct ccgggatcat acggatactt
      361 atcaacagtt gattcagcgt atcagagaat atgccgcact ctttgccgga aagaacattg
      421 aaaaagttgc cattttttca gaaaaccgcc ccgagtggat atatgcgttt tattctgcct
      481 ggtacaatag tgccttggtt gtacccatcg attttctgtc ttcagcggca gatgcagcat
      541 atattctgaa cgactgccgc ccggaactgt tgtttgccgg taaaagtgta tccgctacat
      601 tggcaaagat gtatggcaaa ctggagttta aaccggaaat tatttatttt gaagatgttc
      661 ggctaaccgg agacactatt tattcggagt gggattttca tcccatagag gataaaacgg
      721 cggcaattat ttatacttca ggcacaaccg gaagtcccaa aggggtcatg ttatctttta
      781 aaaacatctc ggcaaatatt aaggcggtaa cagaggatta cccgatcttc accagcgacc
      841 gccccgtatt gatgctgctt cctcttcatc atattttccc tttggccgga tcgatggttg
      901 ctccgctcaa agcaggagga tgtgtagtga tgtctccatc catgcagtca tccgatatta
      961 tcgaaaccct gaaagcccat cctgtgcgga taatgatagg ggtacccagg ctgtatgagc
     1021 ttctttacaa ggggattaaa gctaaaatcg atgccaaagc aattggcagg ctctttttta
     1081 gaatagtcaa actctccagg agcaagaggt tagcaaagaa aatttttaaa aaagttcacg
     1141 ggggcctggg aggcgagctg gagatcatgg tttcgggagg cgctgcgttg aataagacag
     1201 ttggtcaatt ctttgataca ctgggatttg acgtaatgga aggttttgga atgacggagg
     1261 cttctcctat gatcacattt ccaagaccgg gcaaggtggt gatcggcacg gcaggacagg
     1321 cacttccggg actgaaagtt gagatccggg aaggggaagt tgtcgccaaa ggcccaaata
     1381 tcatgcaggg ctattataag aggccggagg aaacggccga ggtactgaga gacggctggc
     1441 tttacacggg tgacctgggg gagatcgatt caaaaggata tctcaggatc acaggcagaa
     1501 agaaagaaat aatcgtgctt ccgaacggga agaatgttaa tcctgtcgaa cttgagcaaa
     1561 aactggaaaa agagtatgcc tgtattaagg aggctggtgt tcttgttcag aatgacatcc
     1621 ttcatgcggt gattctaccc gatttcaatg tgcttgctaa agatggtgta aaagatgcag
     1681 acctgtattt cagaaccgat gtttttcctg cctttaacga agaacttacc tcctataaaa
     1741 ggatcatgca gtatacgctg gtcaaggaag agctaccccg gacccgtctt ggaaaaattc
     1801 agcggtttaa gctggatgaa cttgcagtag gaaaaacgaa agagaaaaga caatcggccg
     1861 aagaggattc ttccgaggag tacctggccg tgaaaacctt cctggaaagc caggtggata
     1921 tgacgatttc cccggaagat caccttgaat tcgacattgc acttgattcg ctgggtaagt
     1981 tgagtcttat cgactatatc gaaaaaacat tcggcgtaaa gattgaagaa gaacacctgc
     2041 caaagttccc acaagtcaaa tcactcgttg aatatattaa aataaataag aaatggcaaa
     2101 aagaagatgt aaccagctgg gcttcagcat tgaaggagaa agtgcaggtc aaactgccca
     2161 gaacctggcc aaccctacct atgataaaaa actcggcaaa aggatttttt gctgtttatt
     2221 ccaggctgaa aagcagggga accgaaaaca ttccggaagg accttgcatt attgccccta
     2281 atcatgagag ttttttcgat ggcctatacc ttgtagcggc cttaaagatg aagaccatga
     2341 aaaagagtta tttctatgct aagaaaacgc atgttaataa ctggttccta aggttcatgg
     2401 caaatcggaa caatgtcata gtcatggatg tgaataaaga tttaaaagat tccatccaga
     2461 aactggccga ggtacttaga aagggcaaga acgtcatcat cttcccggaa gggacccgga
     2521 caaagaccgg caagatggga gatttcaaga agactttcgc catcctcagc aaggaactga
     2581 atatcccggt ggtgcccgtt gccattagcg gcgcttataa agccatgcct tacagggcaa
     2641 tttttccgcg tcctttgagt aagataagta tttgttttgc ggaaccggtt tatccggata
     2701 gtttttccag tgatgaactt accgccatag tcagggacag gatcagcaac atgctcaact
     2761 gccggtgatc agatggtaac cgggcctttg tggcttttcc ttttccataa atagtaaaca
     2821 ggtattcctg aaatgacaat tcccaatccc ggccaggtaa acaggggttt gtaaatgaga
     2881 agaataatga tgatcagcag gcatgctgct atataaatta tcgggaagac aggaaatccg
     2941 aatgctttgt atgggcgttc ccattccggg tgttttattc tgagcatgaa gacggcaaat
     3001 atgaccagga tatagaacag taatgctgca aacacaacat aatcaagtaa ttgactgtaa
     3061 gtacccgtca ggcataagag agttgaccag atggcctgga tccataatcc gaaagccggg
     3121 acacctgttt tgctcagttc acctgttttt ttgaaaaaaa gccggtcttt ggccatggca
     3181 taagtaaccc tggctccgga aagaataatg ccattattac atccaaaggt ggagataaca
     3241 actacaacag ccatcagcaa ttccgtattc tttccgaaaa taccgtaaat agcggcggtc
     3301 gcaaccctgt cggctgtagc aaactggatt cctctttcca taacggtagc accatcgggt
     3361 atccctcgaa ccggcaatgc gatgatatat accacattaa caagaaaata gataaggcag
     3421 accgttaaag ttcccaggaa aagacttcgg gcgattgtct ttttgggatt gatgacctca
     3481 tccgaagtat acgtgatatt gtaccaggca tctgcggcga agatagagcc aaccatcgcc
     3541 aatccgatcg cgatgatcag tgagaaaccc gataccggga cctgctggtt atcaacgatt
     3601 ttggaagctt cccagaatcc gggtgaatga aaaacattca attgtgtagc cttggccacg
     3661 aaaattccga tgacgataaa ggcgagcagg ataaatactt tggaataggt gaaaatattc
     3721 tgcactcttt tcccttcttt gattccccgg gtattgatcc atgtcaggaa aaggatcatc
     3781 ataatggcga gcaattgggt agtgttgaat ttgaataatc ccaggtccag gagaatgttt
     3841 tgttctgaga taaccggcag cagcactcct gtaaattttg caaaagccat ggcgacagcg
     3901 gcaatggtac cacactgaat gaccaggaaa aaggtccatc cgtacagaaa accgaataat
     3961 ggagaatagg cctctttcag gtaaacataa atgccgccga catgtggcag catgctggcc
     4021 agttcgccgt agctcagggc agccagtact gtcatcaggg tggtgacagc ccagaccacc
     4081 agaagccagc ccggggagcc gacattgcgc gcgatatcgg cgctgacgat gaaaataccg
     4141 gagccaatca tggagcccat ggtaatggct gccgaatcga ataggttgag agatttcttg
     4201 aaatggcgtt cggttgccat agggtaatgg gattttgtct gtaaaacaaa tgtggctaaa
     4261 gtcgggctaa gataaaaaaa acattcccgc atcacgaaat ctccttaatt ttactcaaag
     4321 tttaaaatcg atgagcccat ttcagccttg tataatactc gactttaact ttgtcccgct
     4381 gctctttgtc gttgccatag cctggctgat acccatgttg ctgactatat taaaggctga
     4441 ccgggtgcct tccgttctca tagaaatcat tgcaggttat gcagccggcc atttcctcat
     4501 gcagtatgtg gatgccgata gtatgctggt gttgaacttc ctggccctta cggggcttat
     4561 tttcataatg ttccttagcg gactggaaat cgatattaac agcattcttg cttcttttcc
     4621 cagaaaacta agtctgggaa ctctatccaa aaatccaatt ctggcaggta tttcacacta
     4681 cctgatatgc ctggcgatat cactggccgg taccttcctg ctgtcatttt atgttgaaat
     4741 tccgaatgtt tggttttttt ctcttatccc cacgaccaca tttatgggta ttgtgtaccc
     4801 ggtccttaaa aacagaggtg agaccaagac ctattacgga cagaccatca tcaccacatc
     4861 agcgatagcc gatattttca gcataacact tgttaccatt tcagcactgt acctcaaatt
     4921 cggatttgac tatgagttat tcctggtcat tgctttcttt atattttttg tcattttata
     4981 ttatttaggg aagaagtttt ctcccggaat tttcaaaagg atcacttttc aaaaagccca
     5041 tgctgctact caactgagca tgcgaggggc attggcactg gtatttctct ttgttgtgat
     5101 cgcgcagatt gttggtgggg aggtcgtgat cctgggggca tttctgagtg gtttgctgat
     5161 gtcatttttt atgagcaagg agcgttccat attgataatc aagttggaag ggatgggatt
     5221 t
//
LOCUS       DAOQXS010000167         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16146_length_5220_cov_3.728751, whole genome shotgun sequence.
ACCESSION   DAOQXS010000167 DAOQXS010000000
VERSION     DAOQXS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16146_length_5220_cov_3.728751"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKM34_05860"
     CDS             <1..1020
                     /locus_tag="PKM34_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83148.1"
                     /translation="TIARETKALDAWHQGNPSPYKSSTAPNGKVYSPFSFNIDPMERL
                     ILVNFEKDPDEFYTVFEFQQACDIMGRKHLLVIAYRRDDATDVYYQEGYPFGSQDSVL
                     NNACFFIRPLENARFEVNAGLIDVYFAFEDKMGREIIVQIKESKCRKKNPFFLLAPVG
                     ANSEKPMSLPVYSLYQMSFLKQKHSVVEIEIDKVKHKPDTFPLPVDCAKNYFTRYSAD
                     TFNVDWNKNFTGQLSPLIPDKVNIIEDKGIAYEMEETGGHYEIKRMSARNSKHHITIE
                     FSPAFPDIICLREGTDIGGNFSIITDNTTGSIGGHYRIKDKKTKLTCKLIPMEVGNPG
                     RAGWF"
     gene            1186..2781
                     /locus_tag="PKM34_05865"
     CDS             1186..2781
                     /locus_tag="PKM34_05865"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNQ83149.1"
                     /translation="MKKLYILLIVLIITSPNDLSAQVDKSDWVGTVPDGCTSITCGKR
                     ATIDGSVITSHTDDSHRTRSSIFIQPALDHPEGSRCDITQRVPCDTFAMPLYVYKKIG
                     TIPQVKHTFAYVNSAYPCINEHQLAIGESTFGGRESMQSGKGLIDCTMLCQLMVERCT
                     TAREAIAMAGELTAKYGYNDAGECLTIADKKEVWHLEILGPGKDQVGSVWAAQRVPDD
                     HVSVNANASRIRKINLKDKEHFMASENIFQVAIDSGWCTTKDDFEFCYAYAPESRTTL
                     ASRRREWRVLDLVAPSLKLDPNAENYPFSVKPDSLVTLPQLVSIFKDYYEGTPFDMTK
                     DMTVTGDDGKSIISPLANPHMPYDANKLFKINGGWGWLGERTIARWYTMYATIIQCRE
                     WLPDPVGGVVWLAMDNVATSVYIPVYCGVTDLPEPYKVDGRIEGFSNKSAWWAFNRLG
                     TLTAQRWGDMRHDVDAIWNPIQQELFDRQKSVELQAVQMLKEDPGKAKAFLTSYTGMW
                     GSYVVDKAWKLGDYLWTKYDEKF"
     gene            2911..4500
                     /locus_tag="PKM34_05870"
     CDS             2911..4500
                     /locus_tag="PKM34_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNQ83150.1"
                     /translation="MKMRFPHTYVIIFSLIILAAILTWIFPGGDYVEQVKTVDGKEVK
                     ELVFIEQDNAPQTWQVFGTMFKGFVRQAGIIVLILMIGGAFWIMNTTKALDMGILSFI
                     HFTRSLEKFRFFKLIGVDNVVIVLIMLMFSLFGAIFGMSEESIAFVIILVPLAISMGY
                     DSIVGVAMCYLAAHIGFAAAMLNPFTIGIAQGIAGLPLFSGLEYRFFCFIIINIIGIG
                     FVLWYARRIKKNPQRSPVHDIDVYWRDKAAGTTERMEHHTPVSAWVSFALTLAVLIAF
                     SVYYPMTEIKIGNSAAMIIPAIPVATVLFAFLGLISLQKTVHYFILTLLLFTIVFLVI
                     GVMGYSWYIMEIAALFFAMGIASGVAMGYGGNTQVKLFLDGVRDIVSAALVVGLAGGI
                     IVVLEDGKVIDTILHRLASSLHQAGQIQAVGTMYVIQTIINLVIPSGSAKAALTMPIM
                     APFSDLINLSRQATVMAFQFGDGFTNMITPTSGVLIGALGVARIPYDRWFRWVWPIIL
                     IFFILGFLLLIPTVTMELNGF"
     gene            complement(4497..4979)
                     /locus_tag="PKM34_05875"
     CDS             complement(4497..4979)
                     /locus_tag="PKM34_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ83151.1"
                     /translation="MKKALLLISLIFISVRIFSQVEEINIYDPDANAREDIANAVRQA
                     DAGNKHVLLQIGGNWCPWCVRLHHLESSDPQIDSLLKADYIRVMVNVPKEKDKRDYEL
                     LKTLGNPQRFGFPVLVFLNRKGERIHTQDSWYLEQDKAYDREKVIHLFKMWNVVAAGN
                     "
BASE COUNT         1302 a         1247 c         1310 g         1361 t
ORIGIN      
        1 accattgccc gggaaactaa agctctcgat gcttggcacc agggtaatcc gtctccctat
       61 aaatcatcca cagccccgaa cggaaaagta tattcgccat ttagtttcaa tattgatcct
      121 atggagcgtc tcatactggt taattttgag aaggatccgg acgagtttta tactgttttt
      181 gaatttcagc aggcctgcga tataatgggc aggaagcatt tattggtaat tgcttaccga
      241 agggatgacg ccacagatgt ttattatcag gaaggttatc cctttggatc acaagatagt
      301 gttttgaaca atgcctgttt ctttataaga cctcttgaga atgctagatt tgaagtaaat
      361 gcaggtctta ttgatgttta ttttgctttt gaagataaaa tgggccgtga aataatcgtg
      421 caaataaaag aaagcaaatg tcgaaagaag aatcctttct ttttacttgc accggtagga
      481 gcaaactctg aaaaaccaat gtctttgcct gtttattcac tatatcagat gtcattttta
      541 aaacaaaaac attccgtcgt agaaatagaa atagataaag taaaacataa acctgatact
      601 tttcccttac cggtagattg cgcaaaaaac tacttcactc gttattctgc cgatacattt
      661 aatgttgact ggaataagaa tttcacgggt caattatctc ctttaatccc tgacaaggtt
      721 aatataatag aagacaaggg aatagcctat gaaatggaag agacaggcgg acattatgaa
      781 ataaagcgta tgagcgccag gaacagtaaa catcatatta ccatagagtt tagtccggct
      841 tttcctgata ttatttgcct ccgagaagga accgatattg gaggaaactt ttcaatcatc
      901 acggataata caacgggcag catcggtgga cattaccgga tcaaagacaa gaaaacgaaa
      961 ttaacctgca aattaatccc gatggaggtt ggaaacccag ggagagcagg ttggttttga
     1021 agatcttatt ctctgttgtc aggatattca gggaatggcc aatgagctat gtctggaatg
     1081 caaggatcat attggataat acaggccagc caatgatgca atcggggtgg aaaagggttt
     1141 aagcaaacta atccctatat ttgtcttgta acaaaaacat cacttatgaa aaagctgtat
     1201 atcctattaa tcgttctaat tatcacatct ccaaatgatc tctccgccca ggtcgacaaa
     1261 tccgactggg ttggcaccgt gcccgacggc tgcacgtcga tcacctgtgg caaacgggct
     1321 accattgacg gctcagtaat aacatcccat accgacgaca gccaccggac ccgctcgtcg
     1381 atcttcatcc agccggcgct cgatcatccg gagggttccc ggtgcgatat cacccaacgg
     1441 gtgccgtgcg acacgttcgc catgccgctg tatgtttaca agaagatagg gaccatcccg
     1501 caggtgaagc acacctttgc ctatgtcaac tcggcttacc cttgcattaa tgaacaccag
     1561 ctcgccatcg gcgaatcaac cttcggggga agggagtcga tgcaatcggg caaagggctg
     1621 atcgattgca cgatgctatg ccagctgatg gtggagcgat gcacgacggc ccgcgaagca
     1681 atcgccatgg ccggcgagct gacggcaaaa tacggctata acgatgcggg cgaatgcctg
     1741 accatcgccg ataaaaaaga ggtgtggcac ctggagatcc tcggcccggg aaaagaccag
     1801 gtgggatcgg tgtgggccgc ccagcgcgta cccgacgacc atgtgtcggt aaacgccaac
     1861 gccagccgta tccggaagat caacctcaag gataaggaac actttatggc ttcggaaaac
     1921 atcttccagg tagccatcga tagcggatgg tgcaccacga aggacgattt cgagttctgc
     1981 tatgcttacg ctcccgaaag ccgcaccacg ctggcctcac ggcggcgcga atggcgtgtg
     2041 ctcgacctgg tggcgccgtc tctgaagctc gatccgaatg cagagaacta ccctttctcc
     2101 gtaaaacccg acagcctggt tacactgccg cagctcgttt ccatttttaa ggattactac
     2161 gaagggacgc cgttcgatat gacgaaagat atgactgtaa ccggcgatga tggaaaatca
     2221 attatttcgc cgctggccaa cccgcatatg ccatacgacg ccaacaaact ctttaaaatc
     2281 aacgggggat ggggatggct tggtgaacgg accatcgccc gctggtacac gatgtatgcc
     2341 acgatcatcc aatgccgcga atggttgccc gacccggtgg gaggggtggt gtggctggcc
     2401 atggacaatg tggccacttc ggtctatatc ccggtctact gcggcgtgac cgacctgccg
     2461 gaaccctaca aggtcgacgg gaggatcgag ggcttcagca acaagtcggc ctggtgggcc
     2521 ttcaatcgat taggcacgct gacggctcag cgctggggcg atatgcgcca cgatgtcgac
     2581 gccatctgga acccgatcca gcaggagctg ttcgaccggc aaaaatcggt cgaattacag
     2641 gcggtgcaaa tgcttaagga agatcccggg aaggctaagg ctttcctgac ctcctatacc
     2701 ggcatgtggg gaagctatgt ggtcgacaaa gcgtggaaac tgggcgatta cctctggacg
     2761 aaatatgatg agaaattctg aggtcataag ctcgcttcgc tcgcgacatt ggcatgctgc
     2821 gcatgcgtca taagctcgct tcgctcgcgt cataatgttg gtaataagtt tatttttgaa
     2881 ttttgaactt tgaactttga actttgaact atgaaaatga ggtttccgca tacctatgtg
     2941 atcattttca gcctgataat cctggctgcc attttaacct ggatctttcc gggcggggat
     3001 tatgtcgagc aggtcaaaac cgttgatggc aaggaggtta aagagctggt ctttatcgag
     3061 caggataatg caccgcagac ctggcaggtt tttggcacga tgttcaaggg ttttgtgagg
     3121 caggccggta ttattgtttt aatactgatg atcggcggtg cgttctggat catgaacact
     3181 actaaagcgc tcgatatggg cattctgtct ttcattcatt tcacccgcag tcttgagaaa
     3241 ttcaggttct tcaagctgat cggcgtcgat aatgtcgtga ttgtgctgat catgcttatg
     3301 ttcagcttat tcggcgcaat tttcggcatg agtgaagagt cgatcgcatt tgtgatcatc
     3361 ctggtgccac tggctatttc gatgggatat gattcgatag taggcgtagc catgtgttac
     3421 ctggcggccc atatcggttt tgctgctgcg atgctgaatc cgttcactat cgggatagca
     3481 caggggattg ccgggttacc attgttttca gggctggaat accgcttttt ctgctttatc
     3541 ataattaata tcataggaat tggttttgtc ctctggtatg cccgcaggat caaaaagaac
     3601 ccgcaaagat cgccggttca cgatatcgat gtttactggc gtgacaaagc tgccggaacc
     3661 acagagcgga tggagcacca tacgcctgta tctgcctggg tatccttcgc attaacgctt
     3721 gcggtgctca ttgctttctc tgtttattat ccgatgactg aaataaagat cggcaattcg
     3781 gcggcaatga tcattccggc gataccggtt gcaacggtac tttttgcttt tctcgggctg
     3841 atttctttac aaaaaacggt ccattatttc atcctgaccc tgctgttgtt caccattgtt
     3901 ttccttgtaa taggggtgat gggctattcg tggtatatca tggaaatcgc agcgttgttc
     3961 tttgccatgg ggattgcctc cggggtagcc atgggatacg gcggcaacac ccaggtgaag
     4021 cttttcctgg atggcgtcag ggacattgtt tcagccgcac ttgtagtagg gcttgcaggc
     4081 ggcatcatcg tggtactgga agacggaaag gtaatcgaca cgatattgca ccggctggct
     4141 tcatcgcttc accaggcggg ccagattcaa gccgtaggga cgatgtacgt gatccagacg
     4201 atcatcaacc ttgtaattcc ttccggttct gccaaagcgg cccttacaat gccgatcatg
     4261 gcgccgtttt ccgaccttat taacctgtcg aggcaggcga cggtcatggc tttccagttt
     4321 ggcgacggct tcaccaacat gatcacgcca acatccggtg ttttgatcgg agcgctcgga
     4381 gtggccagga tcccttacga caggtggttc cgctgggtct ggccgattat ccttatcttt
     4441 ttcatccttg gattcttact gctgatccca acggtgacga tggagctgaa cgggttttag
     4501 ttgccggctg ccactacgtt ccacatcttg aagaggtgga taaccttttc ccggtcatag
     4561 gctttgtcct gctccaggta ccagctatcc tgcgtatgaa tgcgttcacc cttccggttg
     4621 agaaaaacca gcacgggaaa cccgaaacgc tgcggattgc caagcgtctt gagcaattca
     4681 taatcccgtt tgtctttttc tttgggcaca ttcaccatca cccggatgta atcggctttg
     4741 agcagcgagt cgatctgggg atcggatgat tccaggtggt gcagcctgac gcaccagggg
     4801 caccagttgc cgccgatctg aagaagcaca tgcttgtttc cggcatcggc ctgcctgacg
     4861 gcatttgcga tatcttcgcg ggcattggca tcggggtcgt atatattaat ttcttccacc
     4921 tgggagaaga ttctaacaga gataaagata agactgatca gcaagagggc ctttttcatg
     4981 tttgtaagat atttgttgta tcgcaaagtt tcgcaaaggt agcgcaaagt ttcgcaaagg
     5041 cgaaaaacat tccgcattgc gcattcggca ttttgcattt gatttttcaa ttttcaattt
     5101 tacctgacca cctgaccacc tacaacttca cgatcttccc tgccgctccc tgcccttcac
     5161 ccttcgttct gagctgatag aaatacactc ctgccggcag acccgataca tcccacctta
//
LOCUS       DAOQXS010000168         5191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16265_length_5191_cov_3.146612, whole genome shotgun sequence.
ACCESSION   DAOQXS010000168 DAOQXS010000000
VERSION     DAOQXS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16265_length_5191_cov_3.146612"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKM34_05880"
     CDS             complement(<1..496)
                     /locus_tag="PKM34_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83152.1"
                     /translation="MFREPPFRDFSPFLKILALIAIMIVTFLMVLALGVAISIPFFGK
                     GILSEIAAVTDYQDERIMAALKYFQIVNQIGLFILPAILFVILTDDRVGHYLNLGMSI
                     KRFSTLFGCIVLVISLPFINWMVMINNNIALPEFLSGVEEWMRRSEENARLLTEAFLS
                     ADTWK"
     gene            553..1536
                     /gene="dusB"
                     /locus_tag="PKM34_05885"
     CDS             553..1536
                     /gene="dusB"
                     /locus_tag="PKM34_05885"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNQ83153.1"
                     /translation="MVKIGPIDAGEFPILLAPMEDVSDPPFRYVCKLFGADITYTEFI
                     SSEGLIRDAAKSLKKLEFTEFERPIGIQIFGHDIESMVKAARYAEEANPDIIDINYGC
                     PVKKVAGKGAGSGILNDIPKMVKMTEAVVRAVNKPVTVKTRLGYDNDRKEIVEIAERL
                     QDVGIQAITIHGRTRVTRSSEPADWTLIGKVKENPAMHIPVFGNGDVFTPQAAKFFKE
                     TYHVDGLMIARGSYGNPWIFRDIKHYLATGQILSAPDIHERVKICRIHLEKSVEWKGE
                     RVAVNEIRKHYSDYFKGFPDFKRYKVLLMEESSSEKINQILNDIEHYYSYI"
     gene            1551..2180
                     /locus_tag="PKM34_05890"
     CDS             1551..2180
                     /locus_tag="PKM34_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ83154.1"
                     /translation="MKKLDIAIVDDHTLFRQGLTAALSSEKMINRIFEASNGEEFLEM
                     IKTQSPDVVLMDISMPVLDGIEATRIAVSGNNQLKIIALSMHNDIEHFQDMIEAGVVG
                     FLSKDSALEDVSAAIESVANGNKVFSQDLMYSLVRKMNKEKSQTDLITEREREILQRV
                     SSGKSNNEIAAELNISKRTVDKHRENILAKTQTKNTAELIMFALKNKLI"
     gene            2496..2897
                     /locus_tag="PKM34_05895"
     CDS             2496..2897
                     /locus_tag="PKM34_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ83155.1"
                     /translation="MNQTSKKRILVVDDNLPMRSALSETLKFLNYKTVEAENGQQALE
                     IIEKSCSQPETGEEPELALILCDWSMPILDGRGLFNEMKKRNLDIPFVMLSGYMTPAE
                     AEEMTGTGLAGWLQKPADIRQIANLLEKILH"
     gene            2951..4696
                     /locus_tag="PKM34_05900"
     CDS             2951..4696
                     /locus_tag="PKM34_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ83156.1"
                     /translation="MKKLTPEIRKLFHDHRNTLAVIKLYAQLGYHDQDIPESAREFFN
                     TISGQTAEAVRSIEDLRLLLEEDLFPGDQEANPLLRESDKQLEVLLSNLPGMAYRCKN
                     APGWPMEFISEGCLDLTGYDPEDFIMGKKLQYEDIIHPDDRNYVNDTIQEYVVKDMPF
                     TLEYRIKAKDGTERRVWEKGRQVLGNDNTERRLEGFIMDITERIHVEEELKRNQEKIM
                     SVFRVSPSGIGMVINRMITEINDRICEMTGYSQDELIGQNALILYPSKEDYDYVGTEK
                     YRQIEKTGIGVVETRWKRKNGTVIDILLASTPMVNHDNSQGVIFTALDITNFKKTEAE
                     KRRIEEQLFQARKLEVIGKLAGGVAHEFNNTLQVINTLTELSIMKLDQDHPVAGHLDQ
                     IRTSVRQSSAFIGQLLTFARKQPFNPVIINLNDYIGSILDMLHRLIGDRIKVNWIPGI
                     DLWQTRIDPVQLNQVLINLTINARDAIGDSGTIDVITGNERITAASSKKLQGSRPGEF
                     VRLTIRDNGCGMEKEILQQIFDPFFTTKPVGKGTGLGLSTVYGIVDQNHGFILADSQP
                     GKGSTFHAYFPRSNA"
     gene            4763..>5191
                     /locus_tag="PKM34_05905"
     CDS             4763..>5191
                     /locus_tag="PKM34_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83157.1"
                     /translation="MNLSILKIFFFSLLISALNHSFAQGYLHTSGKYIHDGDGNELIL
                     RGIGTGNWLLNEGYMMKTADFAGTHTQFRTLLTETIGEENTEIYYQHWLDNHFTRHDV
                     DSMKNWGFNSVRVAMHYKWFTLPIEQEPVTGQDTWFEEGFT"
BASE COUNT         1584 a         1120 c         1218 g         1269 t
ORIGIN      
        1 ctttccacgt atctgctgac agaaatgcct ccgtcaacag ccgggcattc tcttccgaac
       61 ggcgcatcca ctcttcaacg ccgctgagga actccggcag ggcaatgttg ttgttgatca
      121 ttaccatcca attgataaac ggaagtgaaa tgaccaatac aatgcaaccg aacagggtgc
      181 tgaaacgctt gatgctcata cccagattca gataatggcc gacacggtca tcggtgagga
      241 tgacaaacag tatggccgga aggatgaaca gcccgatctg gttgacaatc tgaaaatatt
      301 tcagggccgc cataatgcgt tcatcctggt aatcggttac cgcagcgatt tcactcagaa
      361 tcccttttcc aaaaaatggt atggaaatgg caacgcccag cgccagcacc atcagaaaag
      421 tcacgatcat aatggcaatg agtgccagta tcttcagaaa cggcgagaaa tccctgaatg
      481 gcggttccct gaacataact ccggttatgg ccgtaaaaat agtaaatttg cagtctcctc
      541 gacaacgatg caatggtaaa gatcggacct atagatgcgg gcgaattccc tatattgctg
      601 gcgcccatgg aagatgtcag tgatccgcct ttccggtatg tatgcaaatt gttcggcgcc
      661 gatattacct ataccgagtt tatctcttcc gaggggttga tccgggatgc agcaaaaagt
      721 cttaaaaaac tggaattcac cgaattcgaa cggccgatcg ggatacagat cttcggacat
      781 gatatcgaat cgatggtgaa agcggcccgg tatgccgaag aagccaatcc cgacatcatc
      841 gatatcaatt acggctgccc ggtaaaaaaa gttgccggga aaggggccgg atcagggatt
      901 ctgaatgata tccccaaaat ggtgaagatg accgaagcgg tggtcagggc cgttaacaaa
      961 ccggttacgg ttaaaacgcg tctgggttac gacaacgacc ggaaggagat cgtggaaatt
     1021 gccgagaggc tacaggacgt tggcattcag gcgattacta ttcatgggcg cacacgggta
     1081 acccgttcca gcgaaccggc cgactggaca ctgattggta aagtcaagga aaacccggcc
     1141 atgcatatcc ctgtttttgg caacggcgac gtctttacac cccaagcggc aaagttcttc
     1201 aaagagacct atcatgtcga cgggctgatg attgcacgcg gaagttacgg aaatccatgg
     1261 atattcaggg atatcaaaca ttacctggct accggccaga tcctctccgc tcccgacatt
     1321 catgaaaggg taaaaatctg caggattcac ctggaaaaat cggtggaatg gaaaggggaa
     1381 agggtggccg tcaatgaaat ccgaaaacat tacagtgatt atttcaaagg ctttcctgat
     1441 tttaaacgat acaaagtatt attgatggaa gaatcgtcat cagaaaaaat taatcagata
     1501 ctgaatgata ttgaacatta ttattcttat atttgattga tcccattttt atgaaaaaac
     1561 ttgacatcgc cattgtggat gatcacacgc tgtttcgcca gggtcttaca gctgctctct
     1621 cctccgaaaa aatgatcaac cggatctttg aagcttccaa tggagaggag tttctcgaga
     1681 tgatcaagac ccaatcaccc gatgttgtcc tgatggatat cagtatgccg gtattggatg
     1741 ggatagaagc gaccaggatt gccgtatccg ggaataatca actgaaaatt attgctttat
     1801 cgatgcataa cgatatcgag cacttccagg atatgatcga agcaggggtt gtcggttttt
     1861 tatcgaaaga ttctgccctg gaagatgtat cggcagccat cgaatcggta gccaacggga
     1921 ataaagtctt ctcccaggat ctgatgtata gcctggtcag gaaaatgaat aaggaaaagt
     1981 cgcaaaccga cctgattacc gaacgggaac gggaaatcct gcaaagagtt tcttcgggca
     2041 aatccaataa cgagatcgct gccgagctga atatcagcaa aagaacagtt gataaacaca
     2101 gggaaaatat cctcgccaaa acccagacaa aaaacaccgc cgaactcatc atgtttgccc
     2161 ttaaaaacaa actgatttag tattcctcct acgtattctt aaatattaaa ataggtattt
     2221 atactgttgt ttattaacac gcaataccac tatttttgct aactaataat aatagaatac
     2281 tttaagaatt tattgttacg ggtttaatat ttctcatgtt ttaaagttaa ctcataataa
     2341 gttctaataa gaagtgtgtt gattaattgc tgccaaaggt tattgtttcg aaagatacaa
     2401 taaccttttc tttgtttcaa cattaatgtt taaatttaaa gccaatgagg acaagccggc
     2461 atttaaattt ggaacggtta gatcagtctg aattgatgaa tcaaacttca aaaaaaagaa
     2521 tcctggtggt tgatgataat ctgcccatgc gttccgcgtt gtcagagact ctaaaattcc
     2581 tgaattataa aacagtcgaa gcagaaaacg gtcagcaagc ccttgaaatc atcgaaaaga
     2641 gctgcagcca gccggagacc ggtgaggagc cggaactggc gttaatcctt tgcgactggt
     2701 ctatgcccat cctggatggc cggggtttgt tcaacgaaat gaaaaaacga aacctggata
     2761 ttccttttgt catgctttca ggatatatga caccggcgga agcggaagaa atgaccggga
     2821 caggactggc cggatggctg caaaagccgg ctgatatccg gcaaattgcg aatttgttgg
     2881 aaaaaattct ccattgaatt atctttgata tccggtaagc accttattat aacttacacc
     2941 ggaaattttt atgaaaaagt tgactcctga aatcagaaaa ctctttcatg atcacaggaa
     3001 tactttggct gtcattaaac tttatgctca gcttgggtat catgatcagg atattccgga
     3061 atcagcccgg gaatttttta atactatttc cggccagaca gcggaggcgg tccggtctat
     3121 agaagacctg cgcttactgc tggaagaaga tttgtttccc ggtgaccagg aagcgaatcc
     3181 gttgttgcgg gagagcgata agcaactgga ggtcctgctc agcaatttgc ccgggatggc
     3241 ataccggtgc aaaaacgcgc ccggatggcc catggaattt atcagtgagg gctgcctgga
     3301 cctgacaggt tatgacccgg aagactttat catgggaaag aagcttcaat atgaggatat
     3361 tatccatccc gatgaccgga attatgtgaa tgacaccatc caggagtacg tggttaaaga
     3421 tatgcctttc actttggaat acaggataaa ggcaaaagac ggaacggaaa gaagggtatg
     3481 ggagaaggga aggcaggttt tgggaaatga caatacggaa aggcgactgg aaggttttat
     3541 catggatatt accgaaagaa ttcatgttga agaagaactg aaaagaaacc aggagaagat
     3601 catgagcgta ttccgtgtgt ccccctccgg aataggaatg gttatcaaca ggatgatcac
     3661 agaaatcaat gaccgtatct gcgaaatgac cggttattca caggatgaat tgatcggaca
     3721 aaatgcgctc atactttatc cttcaaaaga ggattacgat tatgtcggca cagaaaagta
     3781 caggcagatc gaaaaaaccg gtataggcgt cgttgaaacc cgctggaaaa gaaagaacgg
     3841 gaccgtgatc gacatcctgc tggcatcaac acctatggtg aatcacgata attcacaggg
     3901 tgttattttt actgcactgg atattacaaa ttttaagaaa accgaagcag agaagagaag
     3961 aattgaagaa cagttattcc aggcccggaa actggaggtc attggtaaac tggccggtgg
     4021 cgttgcccat gagttcaaca atactttgca ggtaatcaac acgctcaccg aactttcaat
     4081 aatgaaactg gatcaggatc accctgttgc cggccatctg gaccagatca ggacttcggt
     4141 caggcaatct tcggcattta tcgggcaatt gctgacattt gcgcgcaaac aacctttcaa
     4201 tcccgtcatc atcaatctca acgattacat cggaagtatc ctggacatgc tccaccggct
     4261 gatcggcgac cggataaaag ttaactggat acccggcatt gacctctggc agacgcgaat
     4321 cgacccggta cagctaaacc aggtcctgat caatcttacc atcaatgcca gggatgccat
     4381 cggcgacagc gggaccatcg atgtgatcac cggaaatgag aggatcactg cggcctcatc
     4441 caaaaaatta cagggaagca ggcccggtga atttgtcagg ctgaccatcc gggataatgg
     4501 atgcggaatg gaaaaagaga tacttcagca aatttttgat ccttttttta ccaccaaacc
     4561 cgtcggcaaa gggacaggcc tgggtttatc gacagtttac ggcattgtcg accagaacca
     4621 cggctttata ctggccgata gtcaacccgg taaaggaagt actttccatg cctatttccc
     4681 acgttcaaat gcataattat ttccatccgg agaaaataac ggtattacct ttacaaaaga
     4741 tatatttcag aaatacgatc agatgaattt atccattcta aagattttct ttttttcact
     4801 ccttatttca gcattgaatc attcctttgc acagggttac ctccatactt ccggtaaata
     4861 cattcacgat ggagatggca atgaattgat attaagaggt ataggcaccg ggaactggct
     4921 gctgaacgaa ggctatatga tgaaaacggc cgactttgcc gggacacaca cccaattccg
     4981 gacccttctg acagagacca tcggggagga aaatacggaa atatactacc agcactggct
     5041 cgataatcac ttcacacgcc atgatgtgga ttcaatgaag aactggggat tcaacagcgt
     5101 cagggtggcc atgcattaca aatggtttac cctgcccatt gaacaggaac cggttacagg
     5161 ccaggatacg tggtttgaag aaggttttac g
//
LOCUS       DAOQXS010000169         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16359_length_5167_cov_4.385563, whole genome shotgun sequence.
ACCESSION   DAOQXS010000169 DAOQXS010000000
VERSION     DAOQXS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16359_length_5167_cov_4.385563"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKM34_05910"
     CDS             complement(<1..331)
                     /locus_tag="PKM34_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83158.1"
                     /translation="MRRHLIVIGLILLFSRDAIPGDLVPGDAIPSVGIIKNIPDSTLL
                     EIVQRQTFRFFWHGAHPVSGLALERSNTVMAVHYWDYINEAWDEPNFGKVPFGPTTEQ
                     SVAQDSGS"
     gene            complement(431..3757)
                     /locus_tag="PKM34_05915"
     CDS             complement(431..3757)
                     /locus_tag="PKM34_05915"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNQ83159.1"
                     /translation="MKIKSTNGLEIQFLENGAVKSIEAGPVRINLKPASFLSDPGTNL
                     FLRKRGNVPGFHPLTGPGSGSRFLVADGNYISTGSWEGLDYECRLVLDDEKMSWQWQV
                     EVKNNSGEPFEFDLVYLQDIGLKPVNAGLLNEYYVAQYLERLIFNDEDYGKVVICRQN
                     MKDATGHPWVMIACRNGAESACTDGMQFYGKTYRETGIPEGLISGSLGGEYAGESPVV
                     AVQERPFVLDPGETHRSVFLGIYLPDHPGASAAADLKMLRLPDEMGFRPKGTPSLGTP
                     SLFPLLPVTDLTETEIEYLFGKDRQHAEIVDDKLLSFFSGNNHIILKNKEILADRPHG
                     HIMQAKAGLVPDEEIMATTAFAFGVFNSHISQGNTNFNVLLSICTSQFNQARESGQRI
                     IVEIEGKKYLLGVPSAFEIGLNHCRWIYKFNDCIYQVRTWTSKSAPQINMDFKIVDSE
                     TVKSRVRLWITHDFDPLNKWKIFQGVEDCLYFALPEEDTMIKNKFKDARYKIKIQDNT
                     RFRAGGAELLFPDQRFTGNNLFVLQTEPTGHLSMSVIGEVIKPNDGIVFANVDEQFAK
                     DCSDAWSEWNNLSRGLALISDQKDVAAIREILPWYGTNALTHYLTPYGLEQFGGAAWG
                     TRDVSQGPVDLLLNLGKYKEARQVLLTIFSNQNPDGGWPQWWMFDSYDNIRSDSSHDD
                     IYYWTIIALASYIKTTEDSRILLEELPYYHEDSEKTSEITPLNEHIERLIRMINSSFI
                     PGTSLVPFGGGDWNDSLQPVGKELARRMISSWTVEMNYQAFRQYQAVCRMTGQERMAD
                     RLDEICLKIRSDFNKYLVKDGIVAGYGLLEDDGTISVMLHPSDTRTGIRFSILPMERG
                     ILSGIFTREQAEHHLEIIEKHLKGPDGVRLMDRPLKYKGGIQEIFHRAESSTFFGREI
                     GLMYVHEHIRYAEALAKMGRADAFVVALRQAIPVDYRSIVPMGDIRQANCYYSSSDVH
                     LKTRYEADERYNEVIEGSFTLRGGWRVYSSGPGIYIGLVISRLLGIRIEWGNVIFDPV
                     LPFSMDGLSASMDFMGKKVTFNYSVKEENFGPKAIRINGQDISTFNEDNIFRQGGAVI
                     PVKILDEILTKDFNLIEVLL"
     gene            complement(3869..>5167)
                     /locus_tag="PKM34_05920"
     CDS             complement(3869..>5167)
                     /locus_tag="PKM34_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83160.1"
                     /translation="FYMFSQGRNPRHRGYSGSTFYWYTPLTPEAGRNTIFPLVGRMSR
                     IVKTMETVIVEAERKAEICVLFYPPYYATELERPETGASELAFNASAIRRPAYFDGLL
                     RVLQVMNIDYDMADLTRSTAGSLSRYKQVWAFATDEMNAADQQTLVDYATNGGNLVIC
                     PYLPDREMSQKPCTLLRDSISISPAGTETIDSPLIDLYGLQDIKCANPQAIYDEEKLA
                     GCEIIARTIRGTACGFEKSFFGKGKIIHIGTWLGFDTEGHKPAYKAILERSGARLSRA
                     FSDNENITVRQRFTPSGEGLLFIGNYYNEEHTGKVTYSHPISGDSISIPFDGTTMQWP
                     PLYAVLSPLCLELAKGIRILHCTSDILRITKSEVGIDLLLTGDRDLAGEIVFEGERVD
                     SIKSVWIDREKMIMQQFSNRIVIKYRHKQKEEFFLKLVFQ"
BASE COUNT         1214 a         1338 c         1240 g         1375 t
ORIGIN      
        1 atgatcccga atcctgtgcc accgattgct cagtcgtcgg gccaaatgga actttgccga
       61 agttgggctc atcccaggct tcattgatat aatcccagta atggacggcc atcaccgtat
      121 tgcttcgttc gagagcaagt ccgctgaccg ggtgggcgcc atgccagaag aagcggaaag
      181 tttgccgctg gacgatttcg aggagtgtgc tgtcggggat gtttttaatg atgccgactg
      241 aagggatggc gtcccccggg acgaggtccc ccgggatggc gtcccgtgag aaaagcaaaa
      301 tcaacccgat tacaatcaaa tgtcttctca tcttcataaa gaattgcaag tttcgtttct
      361 ttaaaattaa tttttttctc gaaaccacta ataaacagtt gtaaaaacca cgaatctttt
      421 caggacaaat ttatagcagc acttctatta aattaaaatc ctttgtgaga atctcgtcaa
      481 gtattttgac cgggatgacc gctccaccct gcctaaagat attatcctca ttgaaagtcg
      541 agatatcctg cccgttaatc cgaatagctt tcgggccgaa attttcttcc ttcactgaat
      601 agttgaaagt cactttctta cccatgaaat ccatcgaagc ggaaagcccg tccattgaaa
      661 acggcagcac cggatcgaat atcacattgc cccattcgat cctgatcccc agcaatcttg
      721 agatgaccag cccgatatag atcccggggc cgctcgagta gacccgccag ccgccgcgca
      781 gcgtgaaact tccttcaatc acctcattat acctctcatc cgcctcataa cgggttttta
      841 aatgtacatc ggaactgctg tagtaacagt ttgcctgccg gatatcgccc atcgggacaa
      901 tactccggta gtcgacaggt atggcctgtc gtaatgcaac gacgaacgca tcggctcttc
      961 ccattttcgc taaagcttct gcatagcgga tatgctcgtg tacgtacatc agcccgatct
     1021 cgcggccgaa gaaagtgctg ctttcagccc gatggaagat ctcctggatg ccgcccttgt
     1081 attttaacgg acggtccatc aaccttaccc cgtcaggacc tttgagatgc ttttcgatga
     1141 tttccaggtg atgttctgcc tgctctctgg taaagattcc gctcaggatg cctctttcca
     1201 tgggcaggat actgaacctg atgcctgttc gggtatcgga aggatgcagc ataacactga
     1261 tcgtcccgtc atcttccagg aggccatacc cggccactat gccgtctttc accaggtact
     1321 tgttgaaatc cgaccttatc ttcaggcaga tttcatcgag ccggtctgcc attctttcct
     1381 gaccggtcat ccggcaaacc gcctggtact gtctgaaggc ctggtagttc atttccacgg
     1441 tccaggagga gatcatgcgc cgggccagct ctttgccaac cggctgcagc gaatcgttcc
     1501 agtcgccgcc accgaaaggt acgagcgaag tgcctggaat aaaagagctg tttatcatgc
     1561 ggatcagcct ttcgatatgc tcattcagag gcgtaatttc agaagttttt tcgctgtctt
     1621 catgataata gggaagctct tccaataata ttcttgaatc ttccgtagtc tttatatatg
     1681 aagccagtgc gattatggtc cagtaataaa tatcgtcgtg ggaactgtcg gaacgaatat
     1741 tgtcgtagct gtcgaacatc caccattggg gccagccccc gtcgggattc tggttggaga
     1801 agatggtcag caagacctgc cgggcctcct tgtattttcc gaggttcagg agcaggtcga
     1861 ccggtccctg cgatacatcg cgggttcccc aggcagcgcc accgaattgc tccagcccgt
     1921 atggcgtaag gtaatgtgtg agggcgttcg ttccatacca gggaaggatc tcccggatgg
     1981 ctgcaacgtc tttctgatcg ctgatgagag ccaacccccg actgagattg ttccattccg
     2041 accacgcatc tgagcaatct tttgcaaact gctcgtcgac attggcaaag acaattccat
     2101 catttggttt gatgacttcc ccgataacgc tcatgcttag atgaccggtc ggttcggttt
     2161 gaaggacaaa taaattgtta ccggtaaaac gttgatccgg gaataatagc tctgcaccgc
     2221 cggcccggaa tctggtatta tcctggatct ttatcttgta acgggcgtct ttaaatttgt
     2281 ttttgatcat ggtatcctct tctggaaggg caaaatacag gcagtcttcc accccctgaa
     2341 agattttcca cttattcagc ggatcaaaat catgggttat ccacaatcgc actcttgatt
     2401 ttacggtttc cgaatcaaca attttaaaat ccatattgat ctgcggagca gacttcgacg
     2461 tccaggtgcg gacctggtag atacaatcgt tgaatttgta gatccagcgg cagtggttca
     2521 gacctatttc aaatgcagat ggaacgccca ggaggtattt tttaccttca atttctacaa
     2581 tgatccgctg gcccgattcc ctggcctggt tgaactggct ggtgcagatt gacaggagga
     2641 cattgaagtt ggtattgccc tgcgatatat gtgaattaaa aacgccaaaa gcaaaggcag
     2701 tcgttgccat tatttcttcg tcaggaacaa gtcctgcttt ggcctgcatg atgtgaccat
     2761 ggggcctgtc ggcaagtatt tctttgtttt tcaggatgat atggttgttt ccgctgaaga
     2821 acgagagtag tttatcgtca acaatttctg catgttgtcg atctttaccg aacaggtatt
     2881 caatttccgt ttcggtgagg tcagtaacgg ggagtaatgg gaaaagggat ggcgtcccaa
     2941 gggatggcgt ccctttggga cgaaatccca tctcatccgg cagcctcaac atcttcaaat
     3001 cagcagcagc actagcgccg ggatgatcgg gcaaatagat ccccaggaaa acacttcgat
     3061 gggtttcacc cgggtcaagc acgaaaggtc tttcctgcac cgccaccacc ggcgattctc
     3121 ctgcatattc cccgccaaga cttcctgata tcaacccttc cgggatcccc gtttcacggt
     3181 atgtctttcc gtaaaattgc attccgtcgg tgcaggctga ctccgctccg ttcctgcagg
     3241 ctatcatcac ccagggatga ccggtcgcat ctttcatatt ctgccggcag atcaccactt
     3301 taccgtaatc ttcgtcattg aatatcagcc tctcaagata ctgggcaaca taatattcgt
     3361 tcagtaatcc cgcattcaca ggcttcagcc cgatatcctg gagataaacc aggtcgaact
     3421 cgaaaggttc gccggagttg ttctttactt ccacctgcca ttgccagctc atcttctcgt
     3481 cgtccaggac gagcctgcat tcataatcaa gaccttccca gctacctgtt gagatatagt
     3541 tgccgtcagc gacgaggaaa cggctgccgc ttcccggacc cgttaaagga tgaaaacccg
     3601 gcacattacc tcttttcctg aggaacaggt tcgttccggg atcagagaga aatgacgccg
     3661 gtttcaggtt tatccgtacc gggcccgctt ctatactttt aacagcccca ttttcaagga
     3721 attgaatctc cagcccgttg gtgcttttaa ttttcatttt tgaagtattt taatcacata
     3781 gaccacagaa aatacgcaga ggacactgag gtttcaaatg agacagcctc ttactctatg
     3841 taatctgtgg tttaaaaaaa ttatcaatct actgaaaaac aagtttcaga aaaaattctt
     3901 ccttttgctt atgcctgtat ttgatgacaa tccgattgct aaactgttgc attatcatct
     3961 tttctcgatc aatccacact gattttatac tgtcaaccct ctctccctca aatacgatct
     4021 ccccagccag gtcgcggtca ccggtcagta gaagatcaat gcctacctcg ctttttgtga
     4081 tccggagaat atcagaggtg caatgaagga tccggattcc ctttgcaagt tccaaacaga
     4141 gcgggctaag cactgcataa agcgggggcc actgcatcgt cgtgccgtcg aatggaatcg
     4201 agatggagtc tcctgagatg ggatgactgt acgtcacctt gccggtatgt tcttcattat
     4261 aataatttcc gatgaagagc aacccttcgc cgctcggggt gaatctttgc cttacggtaa
     4321 tgttctcatt atcagaaaat gctctgctta accgggcgcc cgatctttca agaatggctt
     4381 tataggccgg tttgtgccct tcggtatcaa agcccagcca ggtaccgatg tgaatgattt
     4441 ttccttttcc aaaaaaagat ttctcaaacc cacaggccgt tccccggatg gtccgtgcga
     4501 tgatttcgca accggccagc ttttcttcgt catagatcgc ttgtggattg gcgcatttga
     4561 tatcctgcag gccatagagg tcgatcagcg gggagtcgat ggtctctgtg ccggcgggtg
     4621 aaattgagat ggagtctcga agcagggtgc agggtttttg cgacatctcc cggtcaggca
     4681 ggtatggaca gatgaccagg ttgcccccgt tcgtcgcata atcgacaagg gtttgctgat
     4741 cggcggcatt catctcgtcg gtggcaaagg cccacacctg cttgtaccgg ctgagcgatc
     4801 cggcggtcga ccgggtcagg tcggccatat cgtaatcgat gttcatgacc tggagtacgc
     4861 gcagcagccc gtcgaaatat gcgggccggc ggatggcgga ggcgttgaat gccagctccg
     4921 atgcgcctgt ctcgggccgc tccagctccg tggcatagta tggcgggtaa aacagcacgc
     4981 agatctctgc ttttctttcg gcttcgacga taaccgtctc catcgttttt actatgcggc
     5041 tcattcggcc gaccagcggg aagatcgtgt ttcgcccggc ttccggtgtc aatggggtgt
     5101 accagtagaa agtggagccg gaataccccc ggtgccgggg attccgccct tgggagaaca
     5161 tgtaaaa
//
LOCUS       DAOQXS010000170         5158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16392_length_5158_cov_3.494611, whole genome shotgun sequence.
ACCESSION   DAOQXS010000170 DAOQXS010000000
VERSION     DAOQXS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16392_length_5158_cov_3.494611"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..450
                     /gene="rpsL"
                     /locus_tag="PKM34_05925"
     CDS             76..450
                     /gene="rpsL"
                     /locus_tag="PKM34_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNQ83161.1"
                     /translation="MPTIQQLVRKGRAKLIDKSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGAL
                     DTSDVDGRKQRRSKYGAKRPKK"
     gene            470..937
                     /gene="rpsG"
                     /locus_tag="PKM34_05930"
     CDS             470..937
                     /gene="rpsG"
                     /locus_tag="PKM34_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNQ83162.1"
                     /translation="MRKGKSKSRIIVPDPKFGDLMVTKFVNNIMLRGKKNAAFDVFYE
                     AIEIVAQRSKEDGLEVWKKALANVTPAVEVRSRRVGGATFQIPSEIRPARKQSIGMKN
                     MIKFARNRHEKSMQEKLAGEIMAAYKEEGSAFKKKEDMHRMAEANKAFSHFRF"
     gene            979..1879
                     /locus_tag="PKM34_05935"
                     /pseudo
     CDS             979..1879
                     /locus_tag="PKM34_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     assembly_gap    1880..1889
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1890..3070
                     /locus_tag="PKM34_05940"
     CDS             <1890..3070
                     /locus_tag="PKM34_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNQ83163.1"
                     /translation="PFTALAFKIATDAFVGKIAYFRVYSGQVDEGTVVLNANTNKRER
                     LARILQMHANKRNPLPKIAAGDIGVAVGFKEIRTGDTLTDLQHPIVLENIEFPEPVIN
                     VAVEPKTQDDMERMNLALKKLEEEDPTFDVHYDNETGQTIIRGMGELHLEIITDRLKR
                     EYGVGINSGRPQVSYREAILSEVEYREVYKKQTGGKGKFADLTIVMGPADEGVKGLQF
                     IDETRGGVLPKEYIAAIEKGFRTSMQNGILAGFSMISLKVRLLDGATHPVDSDALSFE
                     IAAAQAFRNASRLANSTLLEPIMKLTINTPEEYVGDVAGDINKRRGQIDDVSAMLGYQ
                     IVYAKAPLSELFGYVTTLRTITSGRASGNMEYSHYQPVPKELVDEILYKIKGYYALNN
                     "
     gene            3083..3388
                     /gene="rpsJ"
                     /locus_tag="PKM34_05945"
     CDS             3083..3388
                     /gene="rpsJ"
                     /locus_tag="PKM34_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNQ83164.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKSTGAIVSGPIPLPTD
                     KKIFTVNRSTFVNKKSREQFELSTYKRLMDIYSTTSKTIDALMKLELPSGVEVEIKV"
     gene            3427..4056
                     /gene="rplC"
                     /locus_tag="PKM34_05950"
     CDS             3427..4056
                     /gene="rplC"
                     /locus_tag="PKM34_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNQ83165.1"
                     /translation="MSGLIGKKIGMTSIFDENGKNIPCTVIQTGPCVVTQVRSKEKDG
                     YEAIQLAFDDAKPKHINRPMKGHFAKAGADPKRIVREFTRFEAGHQKKFGDVLKVDVF
                     EEGEYIDVVGTSKGKGFQGVVNRHNFGGVGDTTHGQHNRLRAPGSIGASSYPSRVFKG
                     MRMAGQMGNHRVKMINLQVIKIIPEKDLIIVKGAVPGSNGSYVIIESWS"
     gene            4047..4673
                     /gene="rplD"
                     /locus_tag="PKM34_05955"
     CDS             4047..4673
                     /gene="rplD"
                     /locus_tag="PKM34_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNQ83166.1"
                     /translation="MELDVYNISGGKTSKKVTLNEGIFGITPNDHAIWLDVKLYLANQ
                     RQGTSKAKERSEVSGSTRKLKRQKGTGTARSGDINSPVFRGGGRAFGPRPRDYEFKLN
                     KKLRKLARKSALTYKAQENGIVILEDFNFEEAKTKNFVAMLKNLNLEDKKTLLVMPDT
                     DKNLVLSSRNLKKAKVMRAEMLNTYDILNANNVVIAESSMPIIEEILG"
     gene            4695..4985
                     /gene="rplW"
                     /locus_tag="PKM34_05960"
     CDS             4695..4985
                     /gene="rplW"
                     /locus_tag="PKM34_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNQ83167.1"
                     /translation="MDIILKPIISEKMTEAGEKAVRYGFFVQKKANKLQIKKAVETLY
                     GVQVKSVNTMNYDGDFKSRGTRSGIMVGRTAAYKKAIVTLAEGETIDFYSNI"
     gene            4990..>5158
                     /gene="rplB"
                     /locus_tag="PKM34_05965"
     CDS             4990..>5158
                     /gene="rplB"
                     /locus_tag="PKM34_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNQ83168.1"
                     /translation="MALKKYKPTTPGQRFKVISSFDDITTGTPEKSLLEPIKSTGGRN
                     NQGRRSMRYIGG"
BASE COUNT         1537 a         1114 c         1350 g         1147 t
ORIGIN      
        1 gataatacgg aagacccgat attttcggat caatttataa ttaaaaattt agaatttata
       61 atttaatcta agcgtatgcc aacaattcaa cagcttgtca gaaaaggacg cgctaaattg
      121 attgataaga gcaaatctcc cgcactggat tcatgtcctc agcggcgtgg tgtttgtgta
      181 agggtttaca cgaccactcc caagaagccg aattccgcca tgcgtaaagt ggccagggta
      241 cgactgacca atggaaaaga agtcaactcg tacattcccg gagaaggcca taacctgcag
      301 gaacacagca ttgtgctgat acgtggtggc agggtgaagg acttgccggg tgtgcgttac
      361 cacatcatcc gcggcgctct cgatacatcc gatgtcgacg gcagaaagca gcgcagatca
      421 aagtacggag caaaaagacc taagaagtaa tatttatatc aaataactga tgagaaaagg
      481 caaatcaaaa tcgagaatca tcgttcccga tccaaaattc ggcgacctga tggtgaccaa
      541 gtttgtcaac aatattatgt tgcgtggtaa gaaaaatgcc gctttcgatg ttttttacga
      601 ggcaatcgag atagttgccc agcgcagcaa ggaagacggt cttgaggtgt ggaagaaagc
      661 cctggccaat gttacgcctg ccgttgaggt cagaagccgc cgggttggtg gagccacatt
      721 ccagatccct tcggagatca ggcctgcccg aaagcaatcc atcggtatga aaaacatgat
      781 caagtttgcc cgtaatcgtc atgaaaagtc gatgcaggaa aaactggccg gtgagataat
      841 ggctgcctat aaagaagaag gttcagcctt taaaaagaaa gaagatatgc accggatggc
      901 agaagccaac aaggctttct cccatttcag attttaagtt ttgagtatta atttgaatca
      961 ggtatagagt acaaaaaaat gtcggacagg ttagtacata cccggaacat cggcatcatg
     1021 gcgcacatcg acgcgggcaa gaccacgacg acggagcgta tattgtacta taccggcatc
     1081 aattacaaga tcggcgaagt agatgacggg acggcgacca tggactggat gatccaggag
     1141 caggagagag ggattacgat tacttcggcc gcaaccaccg ttttctggaa agtcgacgga
     1201 caagattata aagtcaatat catcgatacc ccgggacatg tggatttcac cgtggaagtg
     1261 gagcgatcac tccgaattct tgatggagcc attgccattt tctgcgcagt cggcggggtt
     1321 gaaccccagt cggagactgt atggcgccag gcggataaat accaggttcc ccggttggct
     1381 tatgtgaaca aaatggaccg ttccggtgcc gactatttcc gtgtgctgca gcagatcaaa
     1441 gccaagttaa aggctaaccc ggtacccatt caactcccta tcggcgttga agatactttt
     1501 accggtgtgg ttgatctgat tgaaaataag gcctatacat gggaagagga atcctttggc
     1561 ctgaactacc acgaaacgga aatcccggca gatatggcgg atatggttcg tgaataccgc
     1621 accagcctgg tggaaggcgc ggctgaagag agcgaagaac ttcttgaaaa gttccttgag
     1681 aatcacgatt ccattacggc tgaagatata cacctcgctt tgcgcaaagc cactcttgag
     1741 ggaagaattg tcccggtact gtgtggatca tctttccata ataaaggtgt ccagaaactg
     1801 atcgatgcca ttgtgagata tctacccagc cctcttgaca ttccgccggt ggaaggtacc
     1861 aatccattca ctgataaaan nnnnnnnnna gccttttaca gcgcttgctt tcaaaattgc
     1921 aaccgatgct ttcgtcggca aaatagcata tttccgggtc tattcaggcc aggtagatga
     1981 aggcactgta gttcttaacg ccaatacgaa caagagggag cggctggccc ggatcctgca
     2041 gatgcatgcc aacaaacgca acccactgcc gaaaattgcg gccggtgata taggcgttgc
     2101 tgttggattc aaagagatcc gcacagggga tacgctgacc gacctccaac accctatcgt
     2161 tctggaaaat atcgagtttc ccgagcctgt gatcaatgtg gccgttgagc ccaagacgca
     2221 ggacgacatg gaacggatga acctggccct gaagaaactg gaagaggaag acccgacgtt
     2281 tgatgttcat tatgataacg aaaccggtca aaccataatc cgggggatgg gagaactgca
     2341 cctggaaatt attaccgacc ggctgaaacg tgaatacgga gtggggatca attcaggccg
     2401 tccgcaggtt tcataccgtg aagcaatcct atcggaagtt gaataccgcg aagtttataa
     2461 aaagcagacc ggcggaaaag gcaaatttgc cgatttgacc attgtgatgg gaccagctga
     2521 cgaaggtgtg aaaggacttc agtttatcga tgagaccagg ggaggagttc ttccgaaaga
     2581 atacattgca gccattgaaa agggattccg gacatcgatg cagaatggca tcctggccgg
     2641 attttcgatg atcagcctca aggtgcggct tcttgacgga gcgactcacc ctgtcgattc
     2701 tgatgctctt tcgtttgaga ttgctgcggc ccaggcattc cgcaatgcca gccgactggc
     2761 caattcaaca cttctggagc cgatcatgaa gctgaccatc aatacgccgg aagaatatgt
     2821 cggggatgtt gcaggagaca taaataagag gcgagggcag attgatgatg tcagtgcaat
     2881 gctgggatac cagattgtat atgccaaagc cccgttatcg gagttgttcg gatatgtgac
     2941 taccctccgg acgataacca gcgggcgagc ttccggaaac atggaatatt cacattatca
     3001 acctgttccg aaggagctgg ttgatgagat attgtataag atcaaaggat attacgcatt
     3061 aaataattaa attcagataa acgtgagcca gaagattcgc ataaaactga agtcttacga
     3121 tcataacctg gtcgacaagt cggcagagaa gatcgtgaaa actgtcaagt cgacgggagc
     3181 cattgtaagt ggtccgattc cactgccgac cgacaagaag atctttacag taaaccggtc
     3241 aacatttgta aataagaaat ccagggagca gtttgaactt tcgacctaca agaggttgat
     3301 ggatatttac agcacgactt ccaagacgat tgatgcgctg atgaagctgg aactgcccag
     3361 cggcgttgaa gttgagatca aagtatgatg ccggattacg ttttgaacag ataaaaactt
     3421 gagacaatgt caggattaat aggaaagaaa ataggcatga cgagcatctt tgatgagaac
     3481 gggaagaata tcccctgtac cgtcattcag accggtccat gcgtggtcac ccaggtcagg
     3541 agcaaggaga aggatggtta tgaagctatc cagcttgctt tcgacgatgc caagcccaag
     3601 catatcaaca ggccaatgaa aggccacttt gccaaagccg gagcagaccc caagcggatt
     3661 gtacgtgagt ttacccgttt tgaggccggt caccagaaaa agttcggaga cgtactgaaa
     3721 gttgacgtat ttgaagaagg ggagtatatc gatgtagttg gcacaagcaa aggtaaagga
     3781 ttccagggtg tagtgaaccg tcataacttc ggcggtgtcg gtgatacgac acacggccag
     3841 cacaaccggt tgagggctcc cggttcaatc ggagcatcct cctatccgtc gcgcgtattc
     3901 aaaggcatgc ggatggcggg acagatggga aatcatcgcg tgaagatgat caacctccag
     3961 gtaattaaaa ttattcctga gaaagacctt atcatagtaa aaggtgcggt tccgggatca
     4021 aacggatcat atgtaataat tgagagctgg agctagacgt ttataatatt agtggcggca
     4081 aaacctccaa gaaggttacg ctgaatgaag ggatcttcgg aattacgccg aatgatcatg
     4141 ctatctggct tgatgtgaag ttatacctgg ccaatcagcg ccaggggacg agcaaggcga
     4201 aggagcggtc ggaagtatcc ggcagtacgc gcaagctgaa gcgtcagaaa ggtactggaa
     4261 cggcccgttc gggtgatatc aactctccag tatttcgagg aggcggtcgc gcttttggtc
     4321 cccgcccgag agattatgag ttcaagctga ataaaaagct caggaaactg gcccggaagt
     4381 cggcattgac ttataaagcg caggaaaacg gcattgtgat cctcgaggat ttcaattttg
     4441 aagaggcgaa aacaaagaat ttcgtggcca tgctcaagaa ccttaacctg gaagataaga
     4501 agacgttact ggtgatgccc gataccgata agaacctggt attgtcaagc cgtaatctta
     4561 agaaagccaa ggtcatgcgc gctgagatgc tgaacaccta cgacattctc aatgccaaca
     4621 acgtggttat cgcggagagt tcaatgccaa taattgaaga aattttagga tagtaacatt
     4681 ataagctgaa agagatggat atcattctga agccgatcat atccgaaaag atgaccgagg
     4741 ccggtgaaaa ggcggtcagg tatggttttt tcgtacaaaa gaaggccaat aagctacaga
     4801 tcaagaaagc agtggagacg ctttatggcg tccaggtaaa atcggtcaac acgatgaact
     4861 acgacggcga tttcaaatcg cgcggcaccc gttcgggcat catggtgggt cgtactgcag
     4921 cctataaaaa ggcgatcgtt acgttagcag aaggtgaaac aattgatttt tacagcaata
     4981 tctagataaa tggctctaaa aaagtataaa ccgacaactc cgggtcaacg attcaaggtt
     5041 atcagttcgt ttgacgacat tacaacggga acaccggaaa agtccctgct ggaacccatc
     5101 aagagtaccg gcggccggaa caatcagggc agaaggtcaa tgcgttatat tggcggcg
//
LOCUS       DAOQXS010000171         5151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16418_length_5151_cov_4.087520, whole genome shotgun sequence.
ACCESSION   DAOQXS010000171 DAOQXS010000000
VERSION     DAOQXS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16418_length_5151_cov_4.087520"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1425
                     /locus_tag="PKM34_05970"
     CDS             154..1425
                     /locus_tag="PKM34_05970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ83169.1"
                     /translation="MIRLIYQFTRYIILPIGAAIGITAFILFVTEEIRESPRYIRKHT
                     FPSDSIFIDLDKPIMARKGHIIDSVFSELQAKGGFNGCILYAEKGRLVFKKAYGYDNF
                     KQKTRLTTSSSFQLASVSKMFTAMAIMILKEDGLLDYDDSVRKFIPELPYSGVTIRHL
                     LNHRSGIPDYMHFADEFWGQEKPMTNEDMIRVMKINAPPRFFSPDSGFDYCNSNYALL
                     ASVVERITVKPFEDFARENIFEPLGMNHTFIYKLDPAKEIPAFVPVGVSGHKAGRSMP
                     RVEPDFYQNGIVGDKGVYSSVEDLFKWDQALFTGQLVDEATLKEAFTPGSPKFSKWRD
                     NYGFGWRLRADRVNTVYHYGWWKGFRTYFIRDLYQEKTIIVLTNTTRSLSSNLLFEIL
                     DDRRYELGPVCPYTPPPKKPRSKDHRQATND"
     gene            complement(1478..2176)
                     /locus_tag="PKM34_05975"
     CDS             complement(1478..2176)
                     /locus_tag="PKM34_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ83170.1"
                     /translation="MENNELKVLLAEDDKNLGAVLKAYLEAKGYLTTLCINGKEAFDF
                     FNKKKFDFCIVDVMMPVKDGFTLAQEIRKLDKQIPILFLTAKSMQEDKIRGFEIGGDD
                     YLTKPFSMEELLMRMKAILRRVNEKSESEARTSVYKLGKMTFDYNRQILLTASGENKL
                     TSKEAELLRLLCENANDVLDRSVALNRIWQDDSYFNARSMDVYITKLRKYLKEDPSVE
                     LLNVHGIGFKLVLP"
     gene            complement(2180..3607)
                     /locus_tag="PKM34_05980"
     CDS             complement(2180..3607)
                     /locus_tag="PKM34_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ83171.1"
                     /translation="MKQRTVIFAVVMLSVALTGLMVIQGYWIYNAYRVKHANFIRTVN
                     ESAHSVILVMEKLDLVRKLQQPRLLGGEEFPAVAAVDSINQVLLFEMQKIRTRKDLEV
                     FFNKYFMARELMEDRIFSHSIQDNRQPVDLPLVDSLLRNEFAQRNLNTEWSFGIHNPA
                     SDILVFNDEGIYSAELLNPEIGFHFELYPDDMLDNPDFLLLYFPHERQYLLGQVWPLV
                     LISIILVIIIISSFIYTLIMYFRQNRLSELKTDFINNMTHEFKTPVSTISLACEALND
                     KDIKKSEELYQAYINIINEENKRLGLMAERILQSAALEKGDLVLHREVFDIHDILHEA
                     IRNIGIQVEIKDGQIIKDFQAGRSVIEADRMHLVNIVQNLLDNANKYTPVKPQIIVGT
                     KDAANGIIFTVQDNGIGISKADQKKIFEKLYRVPEGNVHNFKGFGLGLSYVKTVVERH
                     GGYIRLDSELKKGTRFEVFLPDKSK"
     gene            4084..>5151
                     /locus_tag="PKM34_05985"
     CDS             4084..>5151
                     /locus_tag="PKM34_05985"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ83172.1"
                     /translation="MNNIKLDLTNIKQFVSEEEIQSLQKEIDLHYPALLNKTGKGNDF
                     LGWVDLPLKSDREIFIRIESDVERLRKISEIFVVTGIGGSYLGARAVIEALGHSFYDL
                     LEERKFPYVVYAGHHLSEDYTAELLEILVKKNYSLAVISKSGTTTEPAIAFRILKNHL
                     EKKYGKAGARERIIAITDKQRGALKGLSDKEGYPTYVVPDDVGGRYSVLTPVGLLPIA
                     MAGFDIRKLVQGAADMEAFCRSARTIGENPVMAYVAARNALYRSGRNVEILVNYLPNL
                     VYLTEWWKQLYGESEGKEGKGIFPAGVSFTTDLHSMGQYIQDGMRIIFETVISVENNK
                     KEVAIPLAEDDADGLNYLAGKR"
BASE COUNT         1304 a         1232 c         1232 g         1383 t
ORIGIN      
        1 ctccagctag ttccaacata tatatcatct agatatttaa ataattgatt tgtaacagcc
       61 tgtaaaattc agaagaaaaa ttggtttgag ttgtacgttt gataagtttt tttttgagga
      121 ttagccctat ttttgccgca attttaaaga tccgtgatac gcctgatata ccaattcacc
      181 cgctacatca tcctcccgat cggggcggct attgggatca cagccttcat tttgtttgtt
      241 acggaagaaa tcagggaatc gcccaggtat atcaggaaac acacatttcc ctccgacagt
      301 atttttatcg atctcgataa acccatcatg gcccggaaag gccatatcat cgacagtgta
      361 ttcagtgagt tgcaggccaa aggaggattt aacggctgca ttctttatgc tgaaaaaggg
      421 cggcttgttt ttaaaaaagc ttacggctac gacaatttca agcagaagac ccggctcacc
      481 acctcttcat cttttcaact ggcttcggtg tcgaagatgt tcacggcaat ggccatcatg
      541 attttgaaag aagatgggct gctggattat gatgacagtg tgcggaaatt tataccggaa
      601 ctgccctact caggggttac catccgccat ttgctgaacc accggtcggg tatccccgat
      661 tatatgcatt ttgccgatga attctgggga caggaaaagc cgatgaccaa cgaagacatg
      721 atccgggtca tgaaaatcaa tgcaccgccc cggtttttct cgcccgacag cggatttgat
      781 tactgcaatt ccaactacgc cctgcttgct tcggtcgttg aaaggattac cgtaaagccg
      841 tttgaggatt ttgcacggga gaacatcttt gaacccctgg ggatgaacca tacgttcatt
      901 tataaacttg atccggccaa agagatcccg gctttcgtcc ccgtcggtgt ttcgggacac
      961 aaggccgggc ggagcatgcc gagggtcgaa cctgactttt accagaacgg tatcgtcggc
     1021 gataaagggg tatattcctc cgtggaagac ctgttcaaat gggaccaggc gctgttcacc
     1081 ggccagcttg tcgatgaagc aacccttaaa gaagccttta cacccggaag cccgaagttt
     1141 tccaaatggc gtgataatta tggtttcgga tggcgtttgc gcgccgaccg ggtcaataca
     1201 gtttatcact acggctggtg gaaaggtttt cgcacctatt ttatccgtga tctttaccag
     1261 gaaaagacta ttattgtgtt gacgaatacg acccggtcgc tttcttcaaa ccttttattt
     1321 gaaattcttg acgacaggcg atatgaactc gggcccgtct gtccttatac ccctccaccg
     1381 aagaagcccc ggtccaaaga ccatcgacaa gcgactaacg actaatcctt cgggctttgc
     1441 cctcaggaca ggcgactgac gactgctttc agattgctca gggaagcacc agtttaaaac
     1501 cgatcccgtg cacattcaac agctctacgg aggggtcttc cttcaggtat ttccttaatt
     1561 tggttatgta gacatccatg ctacgggcat tgaaatagct gtcgtcctgc cagatacggt
     1621 tgagggccac gcttcggtcg agcacatcgt tggcattttc gcaaagcaaa cggagcagtt
     1681 cggcttcttt tgaagtaagc ttattttccc cggaggcggt tagtagaatc tgccggttat
     1741 agtcaaatgt cattttcccc agcttgtaca ccgaggtccg ggcttccgat tcgctctttt
     1801 cattgaccct tcgcaggatg gctttcatcc gcatcagtaa ttcttccatg ctgaaaggtt
     1861 tggtaaggta atcatcgccc ccgatctcga aaccccggat cttgtcttcc tgcatcgatt
     1921 tggccgtcag aaacagtatc gggatctgct tgtcaagctt tctgatctcc tgggccagcg
     1981 taaatccatc ttttaccggc atcatgacat ccacgatgca aaaatcaaat ttcttcttgt
     2041 taaaaaaatc aaaagcctcc ttgccgttga tgcaaagggt ggtcaggtag ccttttgctt
     2101 cgagataggc tttcagcacg gcgccaaggt tcttgtcgtc ctcggccagt aacactttga
     2161 gttcgttgtt ttccattttc tatttggatt tgtcgggaag aaaaacctca aaccgggttc
     2221 ctttttttag ttcgctgtcg agccgtatat agcccccgtg cctctccact accgttttta
     2281 cataacttag tcccagtccg aaacctttaa aattatggac atttccctcc ggtacacggt
     2341 ataatttttc aaatatcttc ttctgatccg ctttactgat cccgatgcca ttatcctgca
     2401 cggtaaaaat gatcccgttc gccgcatcct ttgtcccgac aatgatctgg ggctttaccg
     2461 gtgtgtactt gttggcattg tcgagaaggt tctgcactat atttaccagg tgcatccggt
     2521 cggcttcaat gacgctgcga ccagcctgga aatccttaat gatctgcccg tctttgatct
     2581 ccacctggat gccgatattc cggatcgctt catgcaagat gtcatgaata tcaaacactt
     2641 ccctgtgcag aacgagatcg cctttttcaa gagcagccga ttgcaggatc cgttcggcca
     2701 tcaagcccaa tcgcttattt tcctcgttga taatattgat gtaggcttga tacagttctt
     2761 ccgacttttt gatgtcctta tcattcaaag cctcacaggc cagtgaaatc gtcgacaccg
     2821 gggttttgaa ttcatgggtc atattgttga tgaaatcggt tttcagctcc gacagcctgt
     2881 tttgcctgaa atacatgatc agggtgtaaa taaaggaaga gataatgatg atgaccagaa
     2941 tgatggagat cagcaccagg ggccagacct gtccgagcag gtattgcctt tcgtgaggaa
     3001 aatagagcag cagaaaatcg ggattgtcga gcatatcatc gggataaagc tcaaaatgaa
     3061 agccgatctc cggattcagc aattcggcgc tgtagattcc ttcatcatta aaaaccagga
     3121 tatccgatgc cggattatgg atcccgaagc tccattccgt attaaggttt cgctgtgcaa
     3181 actcatttct cagaagcgaa tcgaccagcg gaagatccac cggctgccga ttgtcctgaa
     3241 tgctatgtga gaatatcctg tcttccatca gttcacgggc catgaaatac ttattgaaaa
     3301 acacctcaag gtctttccgg gttcggatct tctgcatctc aaagagcaaa acctggttaa
     3361 tggaatcgac tgccgctacg gccggaaatt cttctccgcc caggagccgc ggttgctgca
     3421 gcttcctgac gagatcgagt ttctccataa cgagtatcac cgaatgggcc gactcgttca
     3481 ccgtccggat aaaattggca tgtttgactc tataggcatt gtaaatccaa tagccctgta
     3541 tgaccatcag gccggtgagg gcgacggaga gcatgacgac agcgaagatg accgtccgtt
     3601 gtttcatgag tcaaaattac tgaattatag cgcgatacaa gggggtttaa catattttaa
     3661 ctttccttaa atttcgttaa cagttggaac ggattttgtg atatattttt gtctcgctaa
     3721 tccagacaaa tgaatcactg ggttatattg cgcgagaagt ctgattcata attttggttt
     3781 ttggtttatt gggggatgcg gcccgctgcc aggcgggctt catcttttta tagcggtatt
     3841 attgctaatt ttgctcctta gcatgcaaaa attcaaggat aaatttcaaa caaatgaact
     3901 gatgccaacc attgcgcaac tttgcggttt ctttgcgaaa ctttgcgtta cagcttgtta
     3961 taagtttcgc aaagtttcgc tgagtgtatg cagagggtcg caaaggaatt tttttcataa
     4021 tcccggcaaa aactgaccga aaggataaaa cacatttttt atataaactt gatcgcaagg
     4081 catatgaaca acattaaact tgacctgaca aacattaaac aattcgtttc tgaagaagaa
     4141 attcagagcc ttcaaaaaga aatagacctt cattaccctg cgttgctcaa caaaaccggt
     4201 aaaggaaacg atttcctggg gtgggtcgac ctgccgttga aatccgacag ggaaatattc
     4261 atccggatcg aaagcgatgt ggagcgtttg cggaagattt cggagatctt cgtcgtgaca
     4321 ggcatcggcg gatcgtatct cggggcgcgg gccgtgatcg aagccctcgg gcattcgttt
     4381 tacgacctgc tggaggagcg caaattcccg tatgtcgtgt atgccggaca tcacctgagc
     4441 gaggattata cggccgaact gctggagatc ctggttaaaa aaaactattc gctggccgta
     4501 atctccaaat cgggcaccac gaccgagccg gccatcgctt tccgcatcct gaagaaccat
     4561 ctcgagaaaa aatacgggaa agccggtgca cgggagcgga tcatcgccat taccgacaag
     4621 cagaggggag cgttgaaagg actgtcggac aaagagggct atcccacgta tgttgttccg
     4681 gatgatgttg gcggacggta ttcggtcctc acgccggtcg gtttgctgcc cattgccatg
     4741 gccggtttcg atatccggaa gctggtgcag ggggcagccg acatggaagc attttgccgc
     4801 agcgccagga ccatcgggga aaacccggtg atggcctacg tggcagcccg caacgctctc
     4861 tacaggagtg gccggaatgt ggagatcctg gtaaattacc tgccgaacct ggtctatctc
     4921 accgaatggt ggaaacagct ttacggcgaa agcgaaggga aggaaggaaa aggaattttc
     4981 cctgccgggg tgagcttcac caccgacctg cactcgatgg gacaatacat ccaggatggt
     5041 atgcgcatca tttttgagac ggtcatttct gtggagaata ataaaaagga ggttgccatt
     5101 cctttggccg aagacgatgc cgacgggctg aactacctgg ccggcaagcg a
//
LOCUS       DAOQXS010000172         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16453_length_5140_cov_3.281219, whole genome shotgun sequence.
ACCESSION   DAOQXS010000172 DAOQXS010000000
VERSION     DAOQXS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16453_length_5140_cov_3.281219"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1273)
                     /gene="murB"
                     /locus_tag="PKM34_05990"
     CDS             complement(203..1273)
                     /gene="murB"
                     /locus_tag="PKM34_05990"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNQ83173.1"
                     /translation="MPKFDELYNETRMEIFHRYPLKSFNTFGLEVYAREFAVLENEED
                     IAVLVDRHRVAGYRMLLLGGGSNILFSGDYDGFVVKIGLKGKEVIDEDEESVVIRGAA
                     GEDWDDFVGYAVQMGWGGLENLSGIPGTVGSSPIQNIGAYGCEMKDRFESLGAVDLCT
                     GEYSEFDPARCRFGYRDSLFKTALKGRMLITHVDFRLDKKLVPNLSYEALKSKLGHIP
                     PEDLTPQHFRNEVLQIRNSKLPDPHLLGNAGSFFKNPFISVSEFAKLKDLFPGIVSYP
                     VEDKESHRITGYKLAAGWLIEKCGWKGYRDGDAGVHKDQALVLVNYGRATGIDILALS
                     EKIRNSVMEKFGVSLEREVNII"
     gene            1333..2451
                     /locus_tag="PKM34_05995"
     CDS             1333..2451
                     /locus_tag="PKM34_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83174.1"
                     /translation="MKRVIVSVINDLSTDQRVERTCRTLAEMGFRVHLVGRQKRDSIP
                     LAGKPYRQTRMRLLTEKGPLFYAEFNLRLFLFLLFHREGLLVSNDLDTLLPNYLISKL
                     KHIPVVYDSHEHFTEVPELAGRKTVKRIWKSIERMIFPRLKDVFTVNGSLAKIFADLY
                     GVEIKVARNVPFAREYRIEKTRSELGLPEDKKIILLQGAGINIQRGAEEAIAAMEYID
                     DAILLIIGGGDVIDALKKLAGLPPVRGKVHFIPRLAPDVLYRYTVHADIGLTLDKDTN
                     INYRYSLPNKLFDYIQARVPVLASPLPEISRVVTAYDIGMLIPDHNPVNIAARMKDMT
                     GNPERVAQWKENLKFAASELCWEKEKQVLVNVYSKYAG"
     gene            2441..3574
                     /locus_tag="PKM34_06000"
     CDS             2441..3574
                     /locus_tag="PKM34_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ83175.1"
                     /translation="MPDKHLHIISFDIPYPPNYGGVIDVFYKIKTLHRLGIRVHLHCF
                     EYPGRDRTPELDRYCEEVHYYPRKTGLKSMLSLAPYIVSSRKSEELIANLLEDNHPIL
                     FEGLHSCYYLDDRRLAGRIKVYRESNIEHRYYFNLFKVDANLRNRLYFLMASAKLRLF
                     QKVLHHASLMLAVSQHDAEYLQKHFPGKPIFHLPSFHANDEVAILPGKGDYALYHGNI
                     EVPENEYAATYLITRVFNDTAVPLIIAGMKPRERFRRMTELYPNVMLIANPDDEKMFD
                     LIRNAHVNILVTFQATGLKLKLLNTLYNGRFCLVNEAMIKGTSLENLCESGNTAAELK
                     NRLAALFDQEFTLDIAAGRANELKKLYDNTENGQKLIDLIYKL"
     gene            complement(3562..4176)
                     /locus_tag="PKM34_06005"
     CDS             complement(3562..4176)
                     /locus_tag="PKM34_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ83176.1"
                     /translation="MVFSGASLEIGPGEFVYLIGKTGSGKSSLLKAMYAELPVHQGEA
                     VVAGYKLIGIRKREIPMLRRKLGIIFQDFQLLSDRNINDNLMFVLKATGWRNKKEMTE
                     QVKEVLLKVGLATKGFKFQHQLSGGEQQRVAIARSLLNNPEVILADEPTGNLDPETSR
                     EIMKLLRDISHTGCAVLMATHNYLLLNEFSARIIRCEGGKIHSL"
     gene            complement(4240..4812)
                     /gene="rsxA"
                     /locus_tag="PKM34_06010"
     CDS             complement(4240..4812)
                     /gene="rsxA"
                     /locus_tag="PKM34_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNQ83177.1"
                     /translation="MEYFVIIISAIFVSNIVLAQFLGICPFLGVSSKVSTAVGMSAAV
                     IFVIVLATIVTWVVQTYVLVPLEITFLQTISFILIIAFLVQMVEIILKKISPPLYQAL
                     GIYLPLITTNCAVLGVALLVHSKEYDLLESVVFGAAISVGFGLAMVLFSGIREHTELM
                     ATPKGMKGVPIALITAGILSLAFMGFSGLV"
     gene            complement(4824..>5140)
                     /locus_tag="PKM34_06015"
     CDS             complement(4824..>5140)
                     /locus_tag="PKM34_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNQ83178.1"
                     /translation="PSLYDALGIFIPLIVVNCIVLGRAEAFASKNSVFSSLIDGLGMG
                     LGFTLALFILGAVREAIGNASIMGIKFTEGDGILVFVLSPGAFLALGYFIALMNRIKK
                     TS"
BASE COUNT         1403 a         1292 c         1190 g         1255 t
ORIGIN      
        1 atccgggaca ccaggagatt cccatgttgc ttagcgggca taacgaataa tggtgggtaa
       61 aaggatcgga caaagccagg aaagcgagtg ccgcaatcca gatcagtgcc tctttcctgt
      121 accacaggga ccggaaaata atggccactt tttgtctatc cattattctt tcaggctaat
      181 attcaatcaa aaataaacaa aatcagataa tattcacttc ccgctcaaga gatactccga
      241 atttttccat taccgaattc cggatttttt cagaaagggc cagaatatct attccggtgg
      301 ccctgccgta attgaccagc accagggcct ggtctttatg gacccctgca tcgccatcgc
      361 ggtaaccttt ccagccgcac ttctcgatga gccagccggc agccagcttg taccctgtta
      421 tgcgatggct ttccttatct tcaacaggat aggaaactat tcccggaaaa aggtctttca
      481 gctttgcaaa ttcagaaacg ctgataaaag gatttttaaa gaaacttccg gcatttccca
      541 acaaatgcgg atcagggagt tttgaattac ggatttgaag gacttcattc ctgaagtgct
      601 gcggagtaag gtcttccggc gggatatgcc ccagttttga tttcagcgcc tcataggaga
      661 ggttcggaac caacttcttg tccagcctga aatccacatg cgttatcagc attctccctt
      721 tgagtgcggt tttgaagaga ctatcgcggt atccgaaacg gcatcttgcc ggatcgaatt
      781 cggaatattc gcccgtacaa agatcgacgg ctccgagcga ttcaaaacgg tctttcatct
      841 cgcacccata agccccgata ttctggatcg ggctgctacc caccgtcccg ggtattcccg
      901 aaaggttctc cagtcctccc catcccattt gtaccgcata accgacaaaa tcatcccaat
      961 cctcccctgc tgccccgcgg atgactaccg attcttcatc ttcatcaata acctcttttc
     1021 ctttcaaacc aattttaacc acaaaaccgt cgtagtcacc cgaaaacagg atattgcttc
     1081 ctcccccgag caaaagcatc ctgtaaccgg caacccggtg ccggtcgacc agaactgcga
     1141 tatcttcttc attctcaaga acggcaaatt ccctggcata aacctccaga ccgaaagtgt
     1201 tgaatgattt taaagggtac cggtgaaata tctccatgcg ggtttcgttg tacaattcat
     1261 caaatttagg caaatatcgc cattaattgc tattttcgtc atcagtaatg ttcacgggga
     1321 taaacagaga atttgaagag agtcatcgta tcggtaatca atgatctcag caccgatcag
     1381 cgggtggagc ggacctgccg taccctggca gaaatgggat tccgggtgca cctggtcggc
     1441 cggcaaaaaa gggactccat ccctctggcc ggaaagcctt accgccagac ccggatgaga
     1501 ctactgacag agaaaggtcc gctattctat gccgagttca atctgagact ttttctattt
     1561 cttttatttc atagagaagg gctcctggtt tcaaacgacc tcgacacctt attgcctaat
     1621 tatctcatct ccaaattaaa acatattcca gttgtttacg acagtcatga gcatttcacg
     1681 gaggtacctg aactggcggg tcggaaaacg gtgaaaagaa tctggaaatc aatcgaaaga
     1741 atgatatttc cccggctgaa ggatgttttc accgttaacg gatcactggc aaagatcttc
     1801 gcagaccttt acggtgttga aatcaaagtg gccaggaatg ttccgtttgc cagggaatac
     1861 aggattgaaa agacgaggag tgagctcggg ttaccggaag acaaaaagat catcctgttg
     1921 cagggggccg ggatcaatat tcagcgcggg gccgaagaag cgattgcagc gatggaatac
     1981 atcgacgacg caatattgct gatcattggc ggaggggatg tcatcgatgc tttaaaaaaa
     2041 cttgccggtt tacccccggt tcggggaaag gtgcacttta tcccacgcct ggccccggat
     2101 gtgctttacc gctatacggt tcatgccgac atcggcctga cactggataa agacaccaac
     2161 atcaattacc gttacagcct gccgaataag ctcttcgatt acatccaggc gcgggtgccg
     2221 gtgctggcat caccccttcc cgaaatcagc cgggtagtta cagcatacga tatcggcatg
     2281 ctgatccccg atcacaatcc cgtgaatata gccgcccgaa tgaaggatat gacgggaaac
     2341 cccgaacgcg ttgcgcaatg gaaagaaaac cttaaatttg ccgcttcgga attatgctgg
     2401 gaaaaagaaa agcaggttct ggtcaatgtt tattcaaaat atgccggata agcatcttca
     2461 tatcatttca ttcgacattc cttatcctcc gaattacggc ggagtcattg acgtattcta
     2521 caagataaaa accctgcacc ggctgggcat ccgggttcat ctgcattgtt ttgaataccc
     2581 cggaagggat cgtacaccgg agttggatcg ttattgtgag gaggtccatt actatccccg
     2641 aaaaacggga ttaaaatcga tgctgagcct ggctccgtat atcgtctctt cccgcaaatc
     2701 ggaagagctg attgccaatc tattagaaga caatcacccg atacttttcg aaggactgca
     2761 tagctgttat taccttgatg accgcaggct ggcaggaagg ataaaagttt accgggagag
     2821 caatatcgaa caccggtatt atttcaatct cttcaaagtg gatgccaacc tgcggaacag
     2881 gttgtatttt ctgatggcca gtgcgaaact caggcttttc cagaaagtcc tgcaccatgc
     2941 ttcgcttatg ctggccgttt cacaacacga tgccgaatac ctgcagaagc attttcccgg
     3001 aaagcctata tttcacctgc ccagttttca cgccaacgac gaggtggcca ttttaccggg
     3061 aaaaggggat tatgcattgt atcatggcaa tattgaagtt cctgaaaatg aatacgctgc
     3121 cacttacctg atcaccagag tgttcaatga tacagcggtt ccgctcatca ttgccgggat
     3181 gaaaccccgg gaaaggttca ggcggatgac ggagctttat ccgaatgtaa tgctgattgc
     3241 caatccggat gatgagaaaa tgttcgatct tatccggaat gcgcatgtca atatcctggt
     3301 caccttccag gccaccggac tgaaactcaa acttttgaac actctttaca atggccggtt
     3361 ttgcctggtc aatgaggcga tgatcaaggg gacatcgctg gagaatttat gtgaatcggg
     3421 caacacggca gctgagctga agaaccggct ggcggcgcta ttcgatcagg aattcaccct
     3481 ggatatagct gccggcaggg ccaatgaact taaaaaactg tacgacaata cggaaaacgg
     3541 ccagaagctt atcgatctga tttacaagct gtgaatcttc cctccttcgc accggataat
     3601 tcgggcagaa aactcgttca gtaataaata gttatgcgtg gccatcagga ccgcacagcc
     3661 ggtatggctg atatcgcgaa gcagtttcat gatctcccgc gaagtttccg gatcgaggtt
     3721 accggtcggt tcatcggcca ggatgacttc cggattattc agcagcgacc gggcgatggc
     3781 cacgcgttgc tgttcgccgc cggagagctg atgctgaaat ttaaacccct ttgtagcaag
     3841 tccgactttc agcagcacct ctttgacctg ctctgtcatc tctttcttat tgcgccatcc
     3901 ggtggctttc aggacgaaca tcaggttatc gttgatattc ctgtcgctta acaattgaaa
     3961 atcctggaag atgatcccca gttttcttcg gagcatgggt atttctcttt tcctgatgcc
     4021 gatcagcttg tatccggcaa caacagcttc accctgatgg acaggcaatt cggcatacat
     4081 tgccttcagc agggaacttt ttcccgatcc ggtttttccg atcaggtaaa cgaattcacc
     4141 cggtccgatc tcgagcgatg cgccggagaa gaccatagta tcacgttgat ataccgggac
     4201 atcccggaag aagattaccc tttcgttgtt cattttattt tataccagtc cgctgaagcc
     4261 cataaatgca agcgacaaga tgccggctgt aatgagcgcg atcggaacgc ctttcatccc
     4321 tttgggtgta gccatcagtt ccgtatgttc acgaatcccg gaaaacagga ccatggccaa
     4381 tccgaaaccc acggagatag ccgcaccaaa cacaacgctc tcgagcagat catactcttt
     4441 ggagtgcacc aacaatgcca cgccaagaac cgcacaattc gtcgtgatca aaggtaggta
     4501 aattccgagc gcctggtata agggagggga gattttcttc aggatgattt cgaccatctg
     4561 gaccaggaaa gcgataatca gaataaaact gatcgtctga aggaaggtta tttccaaagg
     4621 aaccaataca tatgtctgca ccacccaggt tacgattgta gccagaacaa tgacaaagat
     4681 gacagcggcg ctcataccta cagcagttga tactttgctg ctgactccca gaaatggaca
     4741 gattcccaag aactgggcca ggacaatatt agaaacgaat atagccgaaa ttatgatgac
     4801 aaagtattcc attgtgatca gatttatgat gttttcttaa tcctgttcat gagagcgata
     4861 aaataaccaa gggcaaggaa agcgccgggt gacagaacga aaacgaggat cccgtcaccc
     4921 tcagtgaatt taatgcccat gatcgaagca ttgccaatag cctccctaac ggcgcccaga
     4981 ataaacagtg ccaacgtaaa tccgagcccc attccaagcc catcgatcaa ggaggaaaaa
     5041 acactgttct ttgaggcaaa tgcctcagcg cggcccagca cgatgcagtt caccacgatc
     5101 agcgggataa agatacccag ggcgtcgtat agagaaggtg
//
LOCUS       DAOQXS010000173         5113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16582_length_5113_cov_3.265125, whole genome shotgun sequence.
ACCESSION   DAOQXS010000173 DAOQXS010000000
VERSION     DAOQXS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16582_length_5113_cov_3.265125"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="vorB"
                     /locus_tag="PKM34_06020"
     CDS             complement(<1..894)
                     /gene="vorB"
                     /locus_tag="PKM34_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNQ83179.1"
                     /translation="MGELKLMKGNEAFAEGAIRAGADAYFGYPITPQSEVLEYFMAEK
                     PYEKTGMVVLQAESEVAAINMVYGAAAAGKRAMTSSSSPGVSLKMEGISYMAGAELPG
                     LILNVVRGGPGLGTIQPSQADYFQATKGGGHGDYRLIVLAPSSVQEMFDFVSLSFELT
                     FKYRNPVMMLSDGVIGQMMEKVQLSEPKPRRTDEEVIRECPWATTGKPKTRERNIITS
                     LNLDSAVQERHNLHLQEKYRKIEEHEVRFEEFQSEDAEYLFVAYGSSARIAQKAVQLA
                     RAKGIKAGLIRLITLFPFPKKR"
     gene            complement(917..1147)
                     /locus_tag="PKM34_06025"
     CDS             complement(917..1147)
                     /locus_tag="PKM34_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ83180.1"
                     /translation="MAKVRGVIIVDIERCKGCEVCVGSCPTEVIAMAREVNHKGYHYA
                     FSANPDECTGCTNCAVVCPDGVITVYRKKVEA"
     gene            complement(1301..2614)
                     /locus_tag="PKM34_06030"
     CDS             complement(1301..2614)
                     /locus_tag="PKM34_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNQ83181.1"
                     /translation="MKNTPIDFNVVDKYLNETRIGDLGIASIREIKRLIDLIEKESGQ
                     RYVRMEMGIPGLPPVKIGIEAEKEALDMGIPAIYPDIYGRAELKKEISRFVKLFLNLD
                     VHVDGCIPTVGSMQGSFATFMTLNRMHKDKGQTLLINPGFPVHMQQLRILGIETLSFD
                     VYDYRGDKLRDKLESYLSSGKVSTILYSNPNNPSWICFTDKELRIIAEMADKYNVIII
                     EDLAYFGMDFRHDYSQPGKPPYQPSVAHYTDNYVLLISSSKVFSYAGQRIGMMVISNK
                     IFHTEAPDLLRFYKFSKVGHAMIFGTLYSLSSGTAHTPQFALTAILKAANDGRFNFVE
                     DVKEYGEKARIMKKMFTDNGFRIVYDKDEDKPIADGFYFTFSYPGMTGGEMLHELLYY
                     GISAIALSITGSERTEGVRACVSLVRRDQFPDLEKRLKQFHEDHL"
     gene            complement(2690..4750)
                     /locus_tag="PKM34_06035"
     CDS             complement(2690..4750)
                     /locus_tag="PKM34_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNQ83182.1"
                     /translation="MVNRELTDPKSIVIVGGSNDLQKPGGKILKNIIDGGFNGTLMVM
                     NPKEDIVQGVTSYRNATDLPPVELAVMAIASRHIPETAEFLANEKGTRAFIVISAGFS
                     EENEEGKVLEKKLVEIVNKAKASLIGPNCIGVLTPAYHGVFTLPIPKLSLSGCDFISG
                     SGATACFIMENGIQKGLTFARVFSVGNSAQMGVEDILRHMDESFDPAISPRVKLLYME
                     SVQKPEMLLRHASSLIKKGCRIAAVKAGASEAGSRAASSHTGALASSDLAVSCLLRKA
                     GIIRCQGREELINVASVLMHKELQGKNIGIVTHAGGPAVMLTDALSREGFSVPRIENP
                     KATELLQQLYPGSSVSNPVDFLATGTAEQLGTILDYTDEYFDEIDGIAVIFGTPGLSP
                     IFDVYDVLHKKMQNARKPIYPILPSTYTASEEVRDFLSKGRINFPDEVLFAHALGLVY
                     FTPKPSIVEVPLTENAPVDLLNKFNLQKSGYLPPDMVSRMLDFAGIPRVREALVENHH
                     DLPDVLSSMGGPVVMKVIGPVHKSDVGGVILNIRDVDYAMSSYKKLMSIPEATIVLVQ
                     QMLEGQELFAGIKYEKKFGHILLCGMGGIFIEVLKDFSASLTPLSKAEADEMIRNLRI
                     YPMLKGIRGQKGINLKLFADILVRLSNLVSSFPQIMELDLNPLMASGDKIYAVDARIR
                     IGHT"
     gene            4895..>5113
                     /locus_tag="PKM34_06040"
     CDS             4895..>5113
                     /locus_tag="PKM34_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ83183.1"
                     /translation="MKRTALFIFLVMVSAGIFAQATEPVKAEENPNAPEITFDKLVHD
                     YGTIAFGGDGVSVFKFTNTGKEPLILQQP"
BASE COUNT         1292 a         1280 c         1117 g         1424 t
ORIGIN      
        1 cctcttcttc gggaacggaa acagcgttat cagccggatc aggccggctt tgataccttt
       61 tgcgcgggcg agctgaaccg ctttttgagc gatccgtgca ctggaaccgt atgcaacaaa
      121 cagatattct gcatcttctg actgaaactc ttcaaagcga acctcatgct cctcgatctt
      181 gcggtacttt tcctgaaggt gcagattgtg cctttcctgg acagctgagt cgaggttcag
      241 cgaggtaatg atatttcttt ccctggtttt gggtttgccg gttgttgccc atgggcactc
      301 cctgatcact tcttcgtcgg tccggcgtgg tttcggttct gataactgaa ctttttccat
      361 catctgcccg ataacgccat ccgaaagcat catgaccgga ttgcggtatt tgaatgtcag
      421 ctcgaaagag aggctgacaa agtcgaacat ctcctggacc gaagaaggag ccaggacgat
      481 cagccggtaa tccccatggc caccgccttt ggtagcctgg aaataatctg cctgagacgg
      541 ctggatcgtt cccaggccgg ggccaccgcg aacaacgttg aggatcaggc ccggtaactc
      601 ggcgccggcc atatacgaga ttccttccat tttgaggctt accccgggac tggatgagga
      661 ggtcatcgcc cgtttgcctg ctgctgccgc tccatatacc atgttgatgg ctgctacttc
      721 actttcagcc tgcaggacga ccatgccggt cttttcatac ggcttttcag ccataaagta
      781 ttccaatact tccgactgag gtgtgatcgg gtagccgaaa taagcatccg cacccgccct
      841 gattgcacct tctgcaaagg cttcattgcc tttcatcaat tttaattcac ccatatcttc
      901 tttctctttt ttgcgatcaa gcttctactt tcttccggta tactgtgatg acaccatccg
      961 ggcaaacgac tgcacagttc gtgcaaccgg tacattcatc cggattggcg gaaaatgcat
     1021 aatggtaacc cttatggttc acctccctgg ccatcgcaat tacttcggtc ggacacgaac
     1081 cgacgcagac ttcgcatcct ttacaccttt caatatcaac aattattact cctctgactt
     1141 ttgccatttc cttaaaattt aattgtacac ctattataaa taaaggcgct acgaaattat
     1201 taataaaaga tgagataccg gatcagttca cttttgattt gtgaaaagct taacaattta
     1261 tatttggttt aaataattag cttctcacca aattccggga tcaaaggtga tcttcatgga
     1321 attgtttaag ccttttctcc aggtcaggga actggtccct tctcaccagc gaaacacaag
     1381 ccctgacgcc ttccgtcctc tcgcttcctg tgattgatag cgcaatggca ctgatgccat
     1441 aatacaataa ctcatgcagc atctctcctc cggtcatacc gggatacgag aacgtaaaat
     1501 aaaatccatc ggcaatcggt ttgtcttcat ccttatcata cacaatcctg aacccgttgt
     1561 cagtgaacat ttttttcatg atcctggcct tttctccgta ctctttcaca tcttccacaa
     1621 aattgaaccg gccgtcgttt gcagccttta atatggcagt gagagcaaac tgcggagtat
     1681 gggcggttcc ggaactgagt gaatacaaag tgccgaaaat catggcatgt ccaacctttg
     1741 aaaacttata gaaccttaga agatcagggg cttccgtgtg gaagatctta ttggaaatga
     1801 ccatcatccc gattctctgg ccggcgtagc tgaatacttt ggaacttgag atcagaagga
     1861 cataattatc cgtataatga gcaaccgagg gctggtaggg cggtttgcca ggctgcgaat
     1921 agtcgtgcct gaaatccatc ccgaagtatg ccagatcctc aatgatgata acattatatt
     1981 tatcggccat ttcggcaatt atccggagct ccttgtcggt aaaacaaatc catgacgggt
     2041 tattcgggtt ggaataaagg attgtcgata ctttgcctga actcaggtag gattccagtt
     2101 tgtcacgcag cttatcaccc cggtaatcgt aaacatcaaa agagagcgtt tcaatgccaa
     2161 ggatcctcaa ctgctgcata tgcaccggaa atcccggatt tatgagcaat gtctgccctt
     2221 tgtctttatg catccggttt agggtcataa atgtggcaaa acttccctgc attgagccaa
     2281 ctgtcgggat acagccatcg acatgaacat ccaggttcag gaataatttg acaaagcggg
     2341 agatctcttt ctttaattcc gccctgccat aaatatccgg atagattgcc gggataccca
     2401 tgtcaagtgc ctctttttca gcttctatgc cgattttaac cggaggaagg cccggaatcc
     2461 ccatctccat cctgacatac cgttggcctg actctttttc gatcaggtcg atcagccttt
     2521 ttatttcacg tatggaagcg attcccagat caccaatccg cgtttcattc aggtatttgt
     2581 caacaacatt aaaatcgatc ggggtgtttt tcataataat ttcagttgat atcgtattaa
     2641 atcatttcgg ttcaaatata ttaatttata tcacaaagcc aattttcaat taagtatggc
     2701 ctattcgaat acgggcatcc acggcatata tcttgtcgcc cgaagccatc agcggattca
     2761 ggtcaagttc cattatttgc gggaaactcg acacaaggtt cgaaagccgg accaggatat
     2821 cagcaaacag cttcaggttg atccctttct gaccgcgtat tccctttagc atagggtaga
     2881 ttctgaggtt ccggatcatt tcatccgctt cggcttttga gaggggagtg agggatgcgc
     2941 tgaaatcctt caatacctct ataaatattc ctcccatccc gcacaggagg atatggccga
     3001 attttttctc gtatttaatt ccggcaaaca gttcctgccc ttcgagcatt tgttgtacca
     3061 atactatcgt ggcttcagga atcgacatca gtttcttgta ggatgacatg gcatagtcaa
     3121 catcacgtat attcaggata accccgccaa catccgactt atgtaccggc ccgattactt
     3181 tcatcactac cggaccgccc atgctgctca aaacatccgg taaatcatgg tgattctcca
     3241 ccagggcctc acgtacacgt ggaatgcccg caaaatcaag cattctggat accatgtccg
     3301 gcggcagata accgcttttt tgcaggttga acttattcaa taaatccacg ggagcatttt
     3361 ccgtcaatgg aacttcaacg attgatggct ttggagtaaa gtaaaccaag cccagggcgt
     3421 gtgcaaacaa aacctcatcc gggaaattga tccggccctt tgaaaggaaa tcccttacct
     3481 cttcacttgc tgtatacgta gaaggtaaaa tcgggtagat cggttttcgt gcgttttgca
     3541 tctttttatg cagcacatca tagacgtcga agatcgggct gagccccggc gtgccgaata
     3601 ttaccgcgat accgtcgatc tcatcgaagt attcatctgt atagtcaagg attgtgccta
     3661 attgttccgc cgtgcctgta gccaggaaat caaccgggtt actgaccgat gatccgggat
     3721 agagctgctg cagtaattcc gtcgctttgg ggttctctat ccggggaacg ctgaaccctt
     3781 cccgggatag agcatctgta agcatgaccg ccggcccgcc cgcatgcgtc acaatgccga
     3841 tattctttcc ctgcaattct ttgtgcatca gtacagaggc gacgtttatc agttcttccc
     3901 ttccctggca cctgatgatt cctgctttgc ggagcaggca gctaacggcc agatccgaac
     3961 tggccagcgc accggtatgc gacgaggctg cccggctgcc ggcttctgaa gcccctgcct
     4021 tgactgccgc aatcctgcat cctttcttga tcagtgaaga ggcatgcctg agcaacatct
     4081 ccggcttctg aaccgactcc atatacagca gtttcaccct ggggctgatt gccggatcaa
     4141 aagactcgtc catgtgcctg agaatgtcct caactcccat ctgggcactg ttgccaacag
     4201 agaaaacccg ggcaaaggtg agccctttct ggattccgtt ttccatgata aaacaggccg
     4261 ttgcgcccga tccggatata aaatcacaac cggaaagact gagctttggt attggaagtg
     4321 tgaaaacgcc gtgatatgca ggggtcagaa caccgataca gttggggcca atgagtgaag
     4381 cctttgcttt attgacaatc tcgacaagtt tcttttccag aacctttcct tcttcgtttt
     4441 cttcgctgaa accggctgag atcacaataa aagcacgggt gcccttttca ttggccagga
     4501 attcagcggt ttcgggaata tgcctggagg ctatagccat caccgccagt tcaaccggtg
     4561 ggagatctgt tgcattcctg taggatgtaa cgccctgtac gatgtcttcc ttcgggttca
     4621 ttaccatcaa tgtgccgtta aagccaccat cgataatatt cttcaggatt ttgcctcccg
     4681 gtttttgcag atcgttggaa ccgccaacaa tcacgatact tttcggatcc gttaattccc
     4741 tgtttaccat caagaaaaca attattgtta tttgattaac attactgcgc taaagtataa
     4801 aaatgccatg tgttaaaaaa gtataaaaag taattgtgcc gcgatatttt tttaaataat
     4861 taatactttt gtctgtcaat aaaaatttat aaaaatgaaa agaactgcat tatttatttt
     4921 cctggtgatg gtttcagccg ggatttttgc acaggccact gaaccggtaa aagcagagga
     4981 aaatccaaat gctccagaaa taacctttga taaactggtt catgattatg gaaccattgc
     5041 ttttggaggg gacggtgtta gtgttttcaa attcacaaat acgggaaaag aacccctgat
     5101 cctgcagcag ccc
//
LOCUS       DAOQXS010000174         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16740_length_5078_cov_3.960780, whole genome shotgun sequence.
ACCESSION   DAOQXS010000174 DAOQXS010000000
VERSION     DAOQXS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16740_length_5078_cov_3.960780"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PKM34_06045"
     CDS             <1..1270
                     /locus_tag="PKM34_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83184.1"
                     /translation="DIDITSRFRMSGKGFYQYKSGAEAVQEVPLISISVDSARHTVAT
                     GSIPESLKFMLNPHFAFRGKMDMNSSRKELEFDGGFKTQDECYPGMLKYWVAFDAWIN
                     PDSVRIPVQPPVRDMEGRRVDLALQMSDSEDDIYSSWFTLKKYPKDSAIVAPAGEIYF
                     DEWRGSYRIDQSKDKKLPGFEFNPRSCSFITAGEHNLGLKLDYVSLVSYGDVNYMTVP
                     DSTIANLTLSVNFMFYDGSLTIMADSILKSNLKGLDVTRKSYLDFLDFTLGSESGDYK
                     KDMAGIGRNKRLPDVLNRTMILTDVNLYWNSFTNSYISRGPIGVMSLGRDPVNRYMNG
                     FVELMRRRSGDGLFVYLEIDENRYYFFEYRNGILQALSHDKEFVNRINEVKPEKRSMV
                     LPGADQKYEYMVGDKRRMIDFIRKMQSLSY"
     gene            1280..1930
                     /gene="plsY"
                     /locus_tag="PKM34_06050"
     CDS             1280..1930
                     /gene="plsY"
                     /locus_tag="PKM34_06050"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNQ83185.1"
                     /translation="MTLQIILIVILAYLIGSVPTAVWVGKLFFRLDVREHGSGNAGAT
                     NTIRVLGWKAGLPVFLFDVFKGWFAVKLADFLLTGMMTDETLVFLKIGLAAAVVLGHV
                     FPAFAGFRGGKGVATLLGVGFALFPAAVWIVLAIFIIILLTTGFVSLGSIIGSLVFPF
                     LEIFLFGQENPWLIGLSILVAIFIPITHRNNIKRLIKGKESKFRPTRYGSNASKKM"
     gene            2030..3151
                     /gene="dnaN"
                     /locus_tag="PKM34_06055"
     CDS             2030..3151
                     /gene="dnaN"
                     /locus_tag="PKM34_06055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ83186.1"
                     /translation="MKFIVSSSVLLKNLSALSGVISSSNTLPILDDFLFELKEDQLFV
                     TASDLETTMTVTIPLTKAEEPGSVTIPAKLLLDTLKTFSDIPVIFSINPETLLVELSA
                     GEGKYKLSGHSSDEFPQSPVLEATSVLTVHSGVLVDAINKTLFATGSDELRPVMSGVY
                     CELSPEHLTFVATDAHKLVRYRRMDAHSDQVSSFILPKKPLNQIKGILAGKDLEVNIE
                     YSQTNSMFTFDSFKIICRLIEGKYPNYEAVIPTNNPNVLTVDRGLLLNAIRRVALFAN
                     QSTHQIRFKISGKELVLSAEDIDFSNEAKERLTCSYEGEDMEIGFNSRFLQDMVTNVS
                     SEQVKFEMSAPNRAGLIMPVENENLEEEILMLVMPVMLN"
     gene            3220..3579
                     /locus_tag="PKM34_06060"
     CDS             3220..3579
                     /locus_tag="PKM34_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ83187.1"
                     /translation="MTQDKYIPEMYRMVDAMDTPGFVNLFTADGIFRWANMEPVAGKE
                     NITAFLTGFFQSIKAISHTDLEYWQVPGVRFATGYVNYTRHNNSLLRVPFSVILKFKG
                     DLINEFLIFVDTSELYK"
     gene            complement(3663..4793)
                     /locus_tag="PKM34_06065"
     CDS             complement(3663..4793)
                     /locus_tag="PKM34_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83188.1"
                     /translation="MKRIIFVLPVILLTLQSLAQLKQEIFPPTNLRNGSDYLPSHVDL
                     IWTPPADWQPSRVDRWFEYDRGIYVGGGIGSCPGCPVEIAIRWDSNYFNNYNAVYLTK
                     IRYLLLEAALDHALRVYQSYYGVFDTLFDYPLQQNLVYFQFDTLEFSPITIDKSRELW
                     VGLWVSDMAPGYPLSFGIGPYPQIEGYGNMIKLNPNGNWQTLTEINPELNYTWQLGAY
                     IETPNDAVFYPVFNVFRSIDEQPFEKIHEGEIYDTIFHDDMNEPAQFVVKYYVNSVYK
                     DDSSISSDTLAIWLVNVPENMRNDRVIIYPNPAKDVVTIHSVNNRINSLSLINTKGET
                     VLIKKPGTEKTTLDVSDLPDSFYVVKINTIEGISNSKLLIIK"
     assembly_gap    4875..4974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1323 a         1124 c         1141 g         1390 t
ORIGIN      
        1 cgacatcgat atcacctccc ggttcagaat gtccggcaaa ggattttacc agtataagag
       61 cggcgctgaa gccgttcagg aggtgccttt aatttcgatc tcggtcgatt cggccaggca
      121 taccgtggct acgggctcaa tccccgaaag cctgaaattc atgttaaatc cacattttgc
      181 tttcaggggc aaaatggaca tgaattcgtc gcgaaaggaa ctggagttcg atggaggatt
      241 taaaacccag gatgagtgtt atcccgggat gctcaaatac tgggttgcct ttgatgcctg
      301 gatcaatccc gacagcgtga ggataccggt ccagccgcct gtcagggata tggaaggacg
      361 tcgcgtcgat ctggctttgc agatgtcgga ttcggaagat gatatttact cttcctggtt
      421 cacgctgaaa aagtatccca aagattcagc tatcgtggca ccggccggtg agatctattt
      481 cgatgaatgg agaggtagtt acagaatcga ccagtcgaag gataaaaaac tccccggttt
      541 tgaattcaat ccgcggagct gcagctttat cacagccggc gagcataacc tcggacttaa
      601 actcgattat gtcagcctgg tcagttatgg tgatgtcaat tacatgacag ttcccgattc
      661 gaccatagcc aacctgactc tatccgtcaa tttcatgttc tatgacggga gcctgacgat
      721 tatggccgat tccatcctga aatcaaacct caaaggcctg gatgtcactc gaaaaagcta
      781 cctggatttt cttgacttta ccctgggcag tgaatcgggt gattataaga aggatatggc
      841 gggcattggc cgcaataaac ggctgcccga tgtgcttaac cgcaccatga tcctgaccga
      901 tgtcaatctt tactggaatt cgttcaccaa ttcctatatt tcacgggggc ctattggggt
      961 gatgagcctg ggccgggatc ccgtaaaccg ctacatgaac ggctttgtcg aactgatgcg
     1021 gcgcagaagc ggcgatggtt tgtttgtcta cctggaaata gatgaaaacc gctactattt
     1081 ctttgaatac cgcaacggaa ttcttcaggc cctgtcgcac gacaaggagt ttgtcaaccg
     1141 gatcaatgaa gttaagcccg aaaagagatc gatggtgttg cccggtgccg atcagaaata
     1201 tgagtacatg gttggcgata aaaggaggat gatcgatttc atccggaaaa tgcaatcgtt
     1261 aagttattag ttccaggcta tgacattaca aatcatcctg atcgttatac tggcttacct
     1321 gatcggctct gttcctaccg ccgtgtgggt tggaaaattg tttttcaggc tcgacgtgag
     1381 agaacacggc agcggaaatg ccggggcaac caatactatt cgggtgctcg gatggaaagc
     1441 cggtctgcct gtttttcttt tcgacgtttt caagggctgg ttcgcggtca aactcgccga
     1501 ttttttgctc accggaatga tgacagacga aacccttgtc tttcttaaga tcggcctggc
     1561 tgccgcggtt gtattgggcc atgtttttcc cgctttcgcg ggatttcgcg gtggaaaagg
     1621 agtggccaca ctgctgggtg tgggttttgc actgtttccc gctgcggttt ggatcgtcct
     1681 ggctattttt atcatcatcc tgctgactac cggttttgtt tcgctgggtt caatcatcgg
     1741 atcgctggtc ttccctttcc tggaaatatt tctcttcggt caggaaaacc cctggctgat
     1801 cggattatcg atccttgtag cgatttttat acccattacc catcggaata atattaaaag
     1861 actgataaaa ggaaaagagt caaagttcag gcctacccgt tatgggagta atgcttcaaa
     1921 aaagatgtga aaaaattgtt aagaaagaaa agcaggggta ctgaccctga gatttatttt
     1981 agttattttt acttaattaa ccagatgtta aatcctaata cacaataata tgaaattcat
     2041 tgtttcgagt tcggtccttc ttaaaaacct gtcggcactc agtggtgtga tcagttcgtc
     2101 caataccctt cccattctgg atgatttttt gtttgaactg aaggaagatc aactctttgt
     2161 gacggcttcc gacctggaga ccaccatgac cgttactatc ccgctcacta aagctgagga
     2221 gcccggttct gtgactatcc cggccaagct gcttctggat acgctgaaga cgttttccga
     2281 cataccggtc atcttttcga tcaatcccga aacgttgctg gtggaacttt ccgccggcga
     2341 gggaaagtat aaactcagcg gccatagcag cgatgaattc ccgcaatctc cggtgctgga
     2401 agctacatct gttctcacag tccattcagg ggtcctggtc gatgctatca acaaaacgct
     2461 ctttgccacc ggcagcgatg aactccggcc ggtgatgtcg ggcgtttatt gcgagttgtc
     2521 gcccgaacat ctcactttcg tggctaccga tgcgcataaa cttgtgagat acaggcgtat
     2581 ggatgcccat tccgaccagg tgagctcctt catccttccc aaaaagccat tgaaccagat
     2641 taaaggtatc ctggccggta aagacctgga agtgaatatc gaatattccc agaccaactc
     2701 catgttcacg ttcgacagtt ttaagattat ctgccggctg atcgaaggaa aatacccgaa
     2761 ctacgaagcg gtcattccta ccaacaatcc gaatgtcctg acggtcgacc ggggtttgct
     2821 ccttaatgcg atccgaagag tagccctctt tgctaaccag tcgacccacc agatccggtt
     2881 taagatttca ggtaaagaac tggtcctgtc tgccgaagat attgactttt cgaacgaagc
     2941 aaaggaacga ctgacttgca gttacgaagg cgaagatatg gagatcggat tcaactcgcg
     3001 ctttttgcag gatatggtca ccaacgtttc ttccgagcag gttaaatttg aaatgtcggc
     3061 accaaaccgc gccggcctga tcatgcccgt cgaaaacgaa aaccttgaag aagagatcct
     3121 tatgctggtg atgcccgtga tgctgaacta gtcgttggct taatacgatg accagttgaa
     3181 caatcaccag gggtgtttgt tatgtcaaaa aaacatacaa tgacacagga caaatatatc
     3241 cctgaaatgt accggatggt ggatgcgatg gacactccgg gcttcgtcaa cctttttaca
     3301 gcagatggaa ttttcagatg ggccaatatg gaacctgtcg ctggaaaaga gaatatcacc
     3361 gcttttctga ccggtttttt tcaatccatc aaagccatca gtcataccga cctcgaatac
     3421 tggcaggttc ccggagtaag attcgctaca ggctacgtca attacacccg ccacaacaac
     3481 agtttgctaa gggtgccgtt cagcgtgatc ctgaaattca aaggggatct gatcaacgag
     3541 ttcctgatct tcgtggatac gtcggaattg tataaatagg aaattttgtt tggagaattg
     3601 aaatcaaatc cgttcggcaa tctttagtct tgtccggtat tcaagaaaaa atccaattct
     3661 tattatttaa tgatcagcaa cttggagttt gagataccct caatcgtatt aatttttaca
     3721 acataaaaag aatcgggtag atccgaaaca tcaagtgtgg ttttttctgt tccgggtttc
     3781 ttaatcaaca cagtctctcc tttggtgttg atcaatgata atgaattgat cctgttattt
     3841 accgaatgta tggtgactac atctttcgca gggttggggt atatgataac cctgtcattt
     3901 ctcatatttt caggtacatt taccagccat atcgccaatg tatcggatga tatagaacta
     3961 tcatccttat atacgctgtt tacatagtat ttaacaacga attgagccgg ttcattcata
     4021 tcgtcgtgga aaattgtatc ataaatctcc ccttcatgga tcttttcaaa aggttgttca
     4081 tcgattgacc ggaaaacatt gaaaacaggg taaaatactg catcgttggg ggtctcaata
     4141 taagcaccca gttgccaggt ataattcagc tcgggattta tttcagtcag ggtttgccaa
     4201 ttcccattgg gattcaactt gatcatattg ccataccctt caatctgcgg atatggtccg
     4261 atgccaaatg ataaggggta tcccggtgcc atatcactta cccataatcc tacccataat
     4321 tcccttgatt tatcgatcgt aataggagaa aactccagtg tgtcaaattg gaaataaaca
     4381 agattttgct gcagaggata atcaaataag gtatcgaata cgccataata cgactggtaa
     4441 accctcaggg catgatcaag ggctgcttcc agaagcaggt acctgatttt cgttaagtag
     4501 acagcattgt aattattgaa ataattcgaa tcccaccgga tagcgatttc aaccggacat
     4561 cccgggcagg atccaatacc ccctccgaca taaattcccc tgtcgtattc aaaccatcga
     4621 tcgacccgtg aaggttgcca atccgccggg ggagtccaaa tgagatcaac atgcgacgga
     4681 agataatcgg atccgttccg caaattggtc ggtgggaaaa tttcctgctt gagctgtgct
     4741 aacgattgta atgtgagtaa tattacaggc aaaacaaaga tgattctttt catcgccgtg
     4801 aaatttactc caaatataag aatattttat atgtattcct attcgttctt ctggcctgac
     4861 taggctttaa acttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaact
     4981 ttgaactttg aactttgaac tttgaacttt gaactttgaa ctttgaactt tgaactataa
     5041 ttatgggcta ttgctatgag tatccgcgtc cgtcggtt
//
LOCUS       DAOQXS010000175         5067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16782_length_5067_cov_4.198723, whole genome shotgun sequence.
ACCESSION   DAOQXS010000175 DAOQXS010000000
VERSION     DAOQXS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16782_length_5067_cov_4.198723"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKM34_06070"
     CDS             complement(<1..691)
                     /locus_tag="PKM34_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ83189.1"
                     /translation="MITVIGLISSAFLQVVAAVSALWLLRASRAKVAWIMIFVAFMFM
                     AFRRIVEVMAITHGDISTGLILLSNWLGIIVSAIMAVAVIMIGRILYKLKKAENISHE
                     LETRFTTLFHNSSDEIYLADLKGNLIEANQVACEALGFSRQELLQKNFKDLKTPKYFD
                     TVEPNLKKIIENGRHVYETEHVSKEGKVISLEVKSRVINYMGQEAIISIARETTERKQ
                     MERKILTAVIGA"
     gene            complement(798..1001)
                     /gene="tatA"
                     /locus_tag="PKM34_06075"
     CDS             complement(798..1001)
                     /gene="tatA"
                     /locus_tag="PKM34_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNQ83190.1"
                     /translation="MNTFLPLLSMPSGTEWLLILAVVLLIFGGRKIPELMKGIGKGAR
                     EFKKGMSGDYDEEPKKDVPAKKE"
     gene            1087..3120
                     /locus_tag="PKM34_06080"
     CDS             1087..3120
                     /locus_tag="PKM34_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNQ83191.1"
                     /translation="MKRFSSLFFIFLITFSVKLHAQEKQPALTHEMTPEEELRKHEIG
                     RDFTPTDPPAAPRSIAEFEEMQSVLIRYPFGIPMSLIKEMAEDCKVKTIVASAAEQQQ
                     VLSQYQSAGVNVANCEWLIAPSNSYWTRDYGPWWVADGYSDIGICDFPYNRPRPFDDN
                     IPVVLAQQMGVPLYGMNLIHTGGNWMDDGDGLAASTQLTWTENPTLTHADIDTLVKDY
                     LGINRYILQDDPLDEYIEHIDCWAKFLDVDKILVGQVPPSDYRYQDYEMIANYWAFQT
                     SAYGNLFQVYRVYTPGTYPYTPYTNSLILNKKVFVPITGSQYDDEAIEVYQEAMPGYE
                     IVPVLFGSWENTDALHCRAKGIADIGMLYVNHQPILGNKPFQMDWDLSAEIVPYSGMG
                     LISDSLRCYYKVNEGDYQSVQLEQINGYHYEATLPLVEPGSQVSYYLRAVDLSGRRKN
                     HPYIGAPDPHVFTVGYSALTVVYPDSMIYTSGDQMVTGQTFNVCNYTNSESVINDIES
                     EGFGYFHWYIEGFDMTFPYTMTVNETLPLTVKIDLPVDKAMGYMVTDTLDIYAEDGHY
                     KVVIRVDSDLLSGIANPQISSARIESIAPNPFSSETRISFSLKAEMDVTVAVCNLQGQ
                     IVRALASCEYPAGNHEITWDGKDEAGNEVRSGIYLLKLKTDRGVDYRKLIISR"
     gene            complement(3146..4099)
                     /locus_tag="PKM34_06085"
     CDS             complement(3146..4099)
                     /locus_tag="PKM34_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83192.1"
                     /translation="MKKNIFAFITVLTLSFSGISAQTLDEAGAAFNAGIQFAKDSSYA
                     EAIVSIEKAIEICKKIGDDGMELQIKAEQLLPGTYFNLAKSLFEQKKYNDAIPNFEKS
                     AQYADVMGETKTADASRTYLAGIYYAQGNAEYKNEALDKAIEKYNKALSYKPEYYKAF
                     YGLGLVYKKQENLPLMKENLDKAIALAGDDEKTKGNAQDAAASAYQKAGALTLQAKKY
                     TTSIENLIASQEYNSTEPRTYFYLAIAYNGLSKWDDAITAATKALELQTDDKSDIYFE
                     LAKAQENKGDKASACANYKNVTGGNNVAAAKFQIEQVLKCQ"
     gene            complement(4159..>5067)
                     /gene="lysS"
                     /locus_tag="PKM34_06090"
     CDS             complement(4159..>5067)
                     /gene="lysS"
                     /locus_tag="PKM34_06090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ83193.1"
                     /translation="YLEVETPILQPLYGGAAARPFTTHHNSLDMTLYLRIANELYLKR
                     LIVGGYDGVYEFSKDFRNEGMDRFHNPEFTQMELYVAYKDYEWMMDLVEEMVEKIALD
                     LHGTSHVRVGENLIDFKRPWKRFTMFEAILYYTGLDISGMEEGELRQAAAKLHVDLDP
                     AMGKGKIIDEIFGQLCEPKLIQPTFITDYPVEMSPLAKKHRSKPGLVERFEAICNSKE
                     ICNAFSELNDPIDQRKRFEYQLELGKRGDQESMVLDEDFLRALEFGMPPTAGLGIGID
                     RLTMIMTNSPSIQDVIFFPQMRPERK"
BASE COUNT         1265 a         1209 c         1194 g         1399 t
ORIGIN      
        1 ccgccccgat cacagccgtt aatattttac gttccatctg tttccgctct gtcgtttcac
       61 gcgcaatcga gataatcgcc tcctgcccca tataattgat gaccctgctt ttcacctcca
      121 gggagatcac tttaccctct ttcgaaacgt gctcggtttc ataaacatga cggccattct
      181 caatgatctt tttaaggttc ggttccaccg tatcgaaata cttaggggtt ttcaggtctt
      241 tgaaattctt ctgcagcagt tcctgcctgc tgaatcccag tgcctcgcag gccacctggt
      301 tggcttcgat caggttcccc ttaagatcgg ccaggtaaat ttcatcgctg ctgttatgaa
      361 acagggtggt aaaccgggtt tcgagttcat ggctgatgtt ttccgctttc tttaacttat
      421 aaaggatccg tccgatcata ataacagcca cggccattat agccgaaact atgatcccca
      481 gccagttgga aagaagaatc aacccggttg aaatatcccc gtgagttatg gccatcactt
      541 ccacaatccg cctgaaagcc ataaacatga atgcgacaaa aatcatgatc caggccactt
      601 tggccctgga agcccttaac agccataaag cactgacggc tgcaacaacc tgcaaaaatg
      661 cggaagatat caggccaatt acggttatca taatgatttt gttgtgtatt ataaaaattc
      721 aaacatacga ttaccaataa aaaaaaggct gctcagacgt atgagacagc ctccaaacgg
      781 cttttgcctt aaatatccta ttctttttta gcggggacgt ccttttttgg ctcttcatca
      841 taatcgccgc tcatcccttt tttaaattcc cgggctcctt tacctattcc cttcatcagt
      901 tcgggtattt tcctgccgcc aaaaatcaaa agaactactg ccaggatcag gagccattcc
      961 gttccggaag gcatcgataa aagaggtaaa aacgtattca tattcctgat attacaaaat
     1021 gaaaaacaaa gatacgaaat aaggcatatt aattttctaa ttttgttgaa tagaatgata
     1081 tattttatga aacgattctc atccctgttt tttatctttc tgatcacttt ttctgtgaaa
     1141 ctgcatgcac aggaaaagca gcccgcgctg acgcatgaaa tgacccctga ggaagaactc
     1201 aggaaacatg aaattggacg cgactttacg ccaaccgatc cgccagccgc accccgcagc
     1261 attgctgaat ttgaggagat gcagagtgta ctcatccgtt atccgttcgg cattccgatg
     1321 agcctgatca aggagatggc ggaagattgc aaagtaaaga cgattgttgc gagcgctgcg
     1381 gagcagcagc aggtgctcag ccagtatcaa tcggcagggg tgaatgtagc caactgtgaa
     1441 tggcttatcg caccgtcaaa tagctactgg acgcgcgact acggcccctg gtgggtggcc
     1501 gacggctatt ccgatattgg aatctgtgat ttcccttata accggcccag gcctttcgat
     1561 gataatattc ctgttgttct cgcccagcag atgggagtcc ctctgtatgg catgaacctg
     1621 atccacaccg gtggcaactg gatggatgac ggggacggac ttgctgcatc cacacaactt
     1681 acctggaccg aaaatcccac actgacgcac gccgacatcg atacgcttgt gaaagattat
     1741 ctgggtatta acaggtatat ccttcaggat gatccgctgg atgaatacat tgaacatatc
     1801 gactgctggg ccaagttcct ggacgttgat aaaatattgg tcggacaggt tcccccttcc
     1861 gattaccgtt atcaggatta tgagatgatc gccaattact gggccttcca gaccagcgct
     1921 tacggtaatc ttttccaggt ttatcgtgtt tacacacccg gtacctatcc ctacacgcca
     1981 tataccaact ccctgatcct taataaaaag gtgtttgttc ccattaccgg aagccagtac
     2041 gatgatgagg ccattgaggt ttaccaggag gccatgccgg ggtatgagat tgtcccggtc
     2101 ctttttggca gctgggaaaa tacggatgcg ctccactgcc gggcaaaagg gattgcagat
     2161 attggaatgc tctatgtaaa tcaccagccg atcctgggaa ataagccatt ccagatggat
     2221 tgggacttat cggcagagat cgttccctat tcggggatgg ggttgatcag cgattccctg
     2281 cggtgttatt ataaagtaaa tgaaggggat taccagtctg ttcaactgga gcagattaat
     2341 ggatatcatt acgaagccac tctccctctt gttgaaccgg gcagccaggt gtcgtactac
     2401 ctgcgtgccg ttgatctttc gggaagaaga aagaatcacc cttatatcgg cgcacccgat
     2461 ccgcatgttt tcacagtagg gtattccgct ttaactgtgg tttatcccga ctccatgatc
     2521 tatacctccg gcgaccagat ggtaaccggc cagaccttca atgtttgtaa ttataccaat
     2581 agtgaatcgg tgatcaatga tattgaaagc gagggatttg gatatttcca ctggtatatt
     2641 gaaggatttg acatgacatt tccgtatacg atgactgtga atgaaaccct gccgctaacg
     2701 gtaaagatcg atcttcccgt tgataaggca atgggatata tggtaaccga tacgctggat
     2761 atttatgctg aagatgggca ttacaaggtg gtgataagag tagatagcga tctgctgagc
     2821 ggtattgcca atcctcagat ttcttctgca agaattgaat cgatagcacc caatccgttc
     2881 agcagcgaga ccaggatctc tttcagcctg aaagcagaga tggatgtaac ggttgctgta
     2941 tgtaacctgc aggggcagat tgtcagggcc ctcgctagct gtgaataccc ggcaggaaat
     3001 catgaaatta cctgggatgg gaaagatgaa gcagggaatg aagtacgttc aggaatttac
     3061 ctgttgaaac tcaagacgga cagaggagtt gattaccgga agctaattat ttccagatag
     3121 cagatattct attaattttt agttcttact ggcatttcag cacctgctcg atctggaatt
     3181 tggcagcagc gacattattt ccgccggtga cattcttgta gttggcacat gcagaggctt
     3241 tgtcgccctt attctcctgg gctttagcca gttcaaaata gatatccgat ttgtcgtcgg
     3301 tctgaagctc cagggctttg gttgcagcgg tgatggcatc atcccatttg gaaagcccgt
     3361 tgtaagcgat agccaggtag aaataggtcc ggggttccgt actattgtat tcctgggaag
     3421 cgatcaggtt ttcgatcgac gtcgtgtatt tcttggcttg cagggtaagc gcaccggctt
     3481 tctggtaagc tgaagcagca gcatcctgcg cgtttccttt ggttttctca tcgtcgccgg
     3541 caagtgcgat ggctttgtca aggttttcct tcatcagggg aaggttttcc tgctttttgt
     3601 aaaccaggcc aaggccgtag aaagctttgt aatattcagg tttataggaa agggccttgt
     3661 tgtatttttc gatggctttg tcgagggctt cgttcttgta ttcggcattt ccctgggcat
     3721 aatagatacc ggccaaatat gtcctgctgg cgtcggcagt tttcgtttca cccatcacat
     3781 cagcatactg cgccgacttt tcaaaatttg ggatcgcatc attgtatttt ttttgttcga
     3841 acaagctttt agccagatta aaatatgtgc cgggaagtag ctgttcagcc ttgatctgga
     3901 gttccattcc atcgtcgcca atctttttac aaatttcaat tgccttctca atagaaacaa
     3961 ttgcttcggc ataggatgaa tcttttgcaa attggatgcc tgcattgaag gcagcgccgg
     4021 cctcatcgag agtctgggct gaaattcctg aaaaactgag agttaaaacg gtaatgaatg
     4081 caaaaatgtt ttttttcata gggatcatat ttaaaatgtt gattatcaaa tcgggtgcca
     4141 aaggtagaat atttctaatt atttcctctc gggccgcatc tgggggaaga atattacatc
     4201 ctgtattgac ggagaattgg tcatgatcat cgtgagccgg tcgatcccga tgccgaggcc
     4261 tgcggtcgga ggcataccga actccagagc cctgaggaag tcttcatcca gcaccatcga
     4321 ttcctggtcg cctcttttgc caagctccag ctggtattcg aagcgcttcc gctggtcgat
     4381 cgggtcgttc agctcggaaa aagcattgca gatctccttg ctgttgcaga tggcttcgaa
     4441 ccgctctacc agcccgggtt tgctgcggtg cttcttagcc agcggcgaca tctccaccgg
     4501 gtaatcggtg atgaacgtcg gttggatcag tttcggttcg caaagctgcc cgaatatctc
     4561 atcgatgatc ttacctttgc ccatggccgg atcaagatcg acgtgcagtt ttgcagccgc
     4621 ctgccgcagc tctccttctt ccattccgct gatatccagg cctgtgtagt acaggatagc
     4681 ttcgaacatt gtgaaccgct tccaggggcg tttgaaatcg atcaggttct cacctacccg
     4741 tacatgtgag gttccgtgaa gatcgagcgc gattttctcc accatctcct ccaccagatc
     4801 catcatccat tcataatcct tatacgccac atacagctcc atctgggtga actcggggtt
     4861 atgaaaacgg tccatccctt cgttgcggaa atctttcgaa aattcataaa cgccatcata
     4921 cccgccgacg atcagccttt tcaggtaaag ctcattggcg atgcggaggt agagggtcat
     4981 atcgagcgaa ttgtgatgcg tcgtgaaagg ccgggcggca gctccaccat acagcggctg
     5041 caggataggc gtctctactt ccagata
//
LOCUS       DAOQXS010000176         5031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16928_length_5031_cov_3.261254, whole genome shotgun sequence.
ACCESSION   DAOQXS010000176 DAOQXS010000000
VERSION     DAOQXS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16928_length_5031_cov_3.261254"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKM34_06095"
     CDS             <1..783
                     /locus_tag="PKM34_06095"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83194.1"
                     /translation="GAERLASEDYDKMKGAFLGPAFHNDEILKTLRKYQAEYQYYQDF
                     DCLIKTTAEYIAGGKVAGWFQGRMEYGPRALGNRSILGDARNPEMQKKMNLKIKFREG
                     FRPFAPAVTEEAIAEYFELDRPSPYMLLVVPVNGSRCIPQPEDYDDFGLYEKLYRVRS
                     DIPAVTHVDFSARLQSVSRETNPRFWQLISEFKRQTGCSVLINTSFNVRGEPIVCSPD
                     EAYRCFMRTEMDYLVMGDYLLDKTRQPAEVKNLLQNEVFGMD"
     gene            820..1926
                     /locus_tag="PKM34_06100"
     CDS             820..1926
                     /locus_tag="PKM34_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83195.1"
                     /translation="MKILKSKKVRYWTFMVSVILVILLTFNVVLTFIIHGKLAKSASK
                     KGSWPVGAYIYDKKIGFDFAPNISGPRNRGTYYVKSHQLGYRIGREEDPGTYRPGGLL
                     SLGCSFTYGDEVESEQTFTHLAAAELGIPAYNYGICSFSYIHALLKAEELKETGVLDK
                     LQPQYVVLGCWSGLPDRSRSPFPPLASDNIPLPAAYLDKEGNDLVIRYPMDLENVFDL
                     VGMYRKEGKGWSLKKFSGIFFSAPRIAYLYVKNNMLAGKMNSGGKTSEVTDFEVYDYY
                     FTGIERIFSGSDTRIIMLFMPNTDAEELPDKALIDAIAAHPRVIPVNGLHAIQRYDIS
                     VRDYMRKHPQPPAHKAYAMEIAAAVRVLSGTGQQ"
     gene            2223..3668
                     /locus_tag="PKM34_06105"
     CDS             2223..3668
                     /locus_tag="PKM34_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83196.1"
                     /translation="MKTIIIAVLSLIVQNVFSQITFEKTFGDIDDDFGSAIAVCFDGT
                     YIFTGTVENIYTGDPDIFLAKLDPFGDTLWTRKYINEYHYDYPHDIIQSYDSGYVITG
                     VTYLEDNKVPFILKYDAGGEISWFKKYEDEIPDGYGYAIVQKPDSGFMICGERDFYKL
                     RWYYRPYMLQTNGDGNCTWHQVFEYFGEFAQYSAYSMCMSPENGYVLCGTHRNNLESN
                     IDQAWMFKVSDHNTVSWNIKYGYSDSTTSAADVKLIPGGGYVFCGSIYYGSIIFPNWD
                     INFYLVRTDLNGTEIWRKNIGHGDHEERGRSLDVCDDGGYIIGGESDMGQDHDDILLV
                     KTNDAGDTLWTKSYGGVLNEELADICVAPDGGFLICGSTYSQGIGGSDIYIIKTDPNG
                     VLTGINEKSAKRTGPVIVPNPGNGAGRLQSCYEYESYEIFDINGRLVRESKCITGDKE
                     TIDLGHLSRGVYLIRFKSSTTSTSVKYIKSY"
     gene            3684..>5031
                     /locus_tag="PKM34_06110"
     CDS             3684..>5031
                     /locus_tag="PKM34_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83197.1"
                     /translation="MKPGFIVLVLCSFLIFKTAHSQEPRWEWASFVTSQEKHTTLENI
                     SAGYHNDIYVACAYDTLLVAGDSSMLHPNMISGNNYAVIKYSSEGDFIDAVDIYTIPG
                     SRVYEIFTAVGRKEDPTFAGSFSKRVFFLDTMINHCYTPYIDSPDGLVFQLDQNMNIK
                     WIKTIGGTLTDQVEGLVVSEEGNIFILSDHYANNDFPCYIDFFQQDTLLADRNFISVS
                     GLNSDGILQWRKEFYGSFSPAYLMMGKDSCLYFWGEASSDYIIIENDTIVKPGNTTVE
                     PVFFSSLGTNGIIQHMEFVEFPVRPLELDVSMSGDICFSGNVYDSIIIDGDSINVPQG
                     THYAFVGKFNPHYQPVWYQVIQQTAGNQLGVFDIEACGEDLAFSSYTNQDVQIVDTVF
                     IINYGIESFTGFFDKDGNLSEVTMNETSGEFYTYELALDPCNNVLLSGQMSGKIYLY"
BASE COUNT         1412 a         1084 c         1159 g         1376 t
ORIGIN      
        1 ggcgcagaac ggcttgcttc tgaagattat gacaaaatga aaggggcatt tttaggcccg
       61 gcattccaca acgacgagat tctgaaaacc cttcggaaat accaggctga atatcaatat
      121 tatcaagact ttgattgcct gataaaaacg acggcagaat atatcgcagg gggcaaagtg
      181 gccggttggt tccagggacg gatggaatac gggccgcgtg cgctgggaaa ccgcagtatc
      241 cttggggatg cccgcaatcc ggaaatgcag aagaaaatga acctcaagat caagttccgc
      301 gagggtttca ggccttttgc accggctgtg accgaagaag ctattgctga atatttcgag
      361 ctcgaccgcc cctctcctta catgctgctg gtggttccgg tcaacggatc cagatgcatc
      421 cctcaacccg aagattacga cgatttcggc ctgtatgaaa aactctaccg ggtccggtcc
      481 gatattcccg ccgtcaccca tgtggatttc tcggcacgac ttcaaagtgt aagccgggag
      541 accaaccccc ggttctggca attgatcagt gaatttaaga ggcaaaccgg ttgcagcgtg
      601 ctgataaata ccagcttcaa tgtcaggggc gaaccgattg tctgctcccc ggatgaggct
      661 taccgctgct tcatgcggac tgagatggat tacctggtga tgggtgatta cctgctcgat
      721 aaaaccaggc agccggctga agtaaagaat ttgcttcaaa atgaggtctt tgggatggat
      781 taagaacctg tatatttcat attatctgat agttgtatta tgaaaatatt gaaatccaaa
      841 aaggtgagat actggacctt catggtcagc gtgatcctgg tcatattact gacattcaat
      901 gtagtgctca cttttataat tcacggtaaa ctggcaaaat cggcttcaaa aaagggcagc
      961 tggccggtcg gggcctatat ctatgacaaa aagatcgggt ttgatttcgc gcccaatatc
     1021 tcagggcccc ggaaccgggg aacctattac gtaaaaagcc atcagctcgg ataccggatc
     1081 ggcagggagg aagaccctgg aacttatcgg cccggcggac ttctgtcgct gggttgctcg
     1141 ttcacttatg gcgacgaagt ggaatcggag cagacattta cccacctggc agcggctgaa
     1201 ctgggcatcc cggcttacaa ctacggcatc tgctctttct cttacatcca cgctttgctc
     1261 aaggcggaag agctaaagga aacaggcgtg ctcgacaaac ttcagccgca atacgtcgtg
     1321 ctgggatgct ggagcgggtt gcctgaccgc tcccggtcgc cctttcctcc tttagcttcc
     1381 gacaatatcc ctttgcccgc agcttacctc gacaaagaag gaaatgacct ggtaatcagg
     1441 tatcccatgg atctggagaa tgtttttgat ttagtcggga tgtaccggaa ggaagggaaa
     1501 ggatggagtt taaaaaaatt ttccgggatt ttcttttccg caccccggat tgcttatctc
     1561 tatgtaaaaa acaacatgct ggccgggaag atgaactccg gtggaaagac aagtgaagta
     1621 accgattttg aagtgtacga ttactacttc accggtattg aaaggatatt ctccggatcc
     1681 gatacacgga tcatcatgct tttcatgcca aacacggatg cggaagaact gccggataaa
     1741 gcgctgatcg acgccattgc cgcccatccg cgcgtcatac cggtaaacgg actgcacgcc
     1801 attcagcgat acgacatttc ggtccgcgat tacatgcgaa aacatccgca accgccggca
     1861 cacaaggcct atgcaatgga gatcgctgcg gcggtgaggg tactgtccgg aacaggccag
     1921 caatgattag caggaggtta cctcaacacg atcaccgaac ccactttcac gatttcaatt
     1981 tcacctgaga tttcactgtt tcagtaaatc cgttgagttt attagaaaat atattctccg
     2041 ggtttccaac agaaaatata aatatatttc atatctttat gaggtcttat agtcggtcag
     2101 atgttgcacc gaagtttcat ccaattctat actatcatcc tggcaaccat ttcatctccc
     2161 tcccaggatt taaaagattt ttatgaatag caattctgaa aaattaacca aatcccaatt
     2221 aaatgaaaac cattattatt gctgtcctct cactaattgt tcagaatgta ttttcacaaa
     2281 ttacatttga gaaaactttc ggcgatattg atgatgattt tggttcagcc atagctgttt
     2341 gtttcgacgg aacttatatc ttcacaggca ctgtcgaaaa tatttatacc ggagatccgg
     2401 atatatttct ggcaaaactt gacccttttg gtgacaccct ttggaccaga aaatatatta
     2461 atgaatacca ctacgactat ccacacgata taatccaaag ctatgattca ggatatgtga
     2521 ttacaggggt aacatatttg gaggataata aagtgccctt tattttaaaa tacgatgctg
     2581 gcggggagat aagctggttc aaaaaatatg aagacgaaat tcccgatggc tacggctatg
     2641 ccattgtcca gaaacctgat tcgggattta tgatctgtgg cgagagagac ttttataaac
     2701 tgagatggta ttaccgcccc tatatgctcc aaacaaatgg cgacggaaac tgtacctggc
     2761 accaggtttt tgaatatttc ggagaatttg cacaatatag tgcctacagt atgtgtatgt
     2821 caccggaaaa cggatatgta ctctgcggaa cccatagaaa taatctggaa agtaatatag
     2881 accaggcctg gatgtttaag gtcagtgacc acaataccgt ctcctggaat attaaatatg
     2941 gttattcaga ttctaccacc tctgcggcag atgtgaaatt gattcctgga ggagggtatg
     3001 tattttgcgg atcaatttat tacggaagca taatatttcc aaattgggat atcaatttct
     3061 accttgtcag aacagatctg aacggtaccg aaatttggcg gaaaaatatc ggtcatggtg
     3121 accatgaaga gcggggtcgc agccttgatg tttgtgatga tggcggttat ataataggcg
     3181 gtgaatcaga catgggtcag gaccatgacg atatattact cgttaaaaca aatgatgccg
     3241 gtgatacttt atggaccaaa tcatatggcg gagttctaaa tgaagagctg gcagacattt
     3301 gtgtggcacc ggacggcgga tttttgattt gcggaagtac gtatagccag ggaatcggag
     3361 gatccgacat ctatattatt aaaacagatc cgaacggtgt gttgacaggc atcaacgaaa
     3421 aatctgcaaa acgtacaggc ccggtcattg ttcctaatcc gggaaatggg gcaggtaggc
     3481 tgcaaagttg ttatgaatat gaaagttatg aaatatttga catcaacggc cgattggttc
     3541 gggaatccaa atgtatcacc ggtgacaaag aaacaatcga cctgggtcat ttgtcacgcg
     3601 gcgtctattt aattcgcttt aaatcatcga ccacatcaac atcggttaaa tatatcaaat
     3661 cctattgata aaatattctt ctcatgaaac caggttttat agttttagtc ttatgctcct
     3721 ttcttatttt caaaaccgct catagccagg aaccaagatg ggaatgggcc tcttttgtta
     3781 cctctcagga gaaacatact acccttgaaa atatttctgc cggataccac aatgatatat
     3841 atgtggcttg cgcttacgac acactgctgg tggccggtga ttcttcgatg cttcatccga
     3901 acatgatcag cggcaataac tatgcagtga tcaaatatag ctccgaaggt gattttattg
     3961 atgcagtcga catctatacc atcccgggct caagagttta tgaaatcttt actgccgttg
     4021 gccggaaaga agatccgact tttgccggat ctttttcaaa acgtgtgttt tttctggata
     4081 ccatgataaa ccattgttat actccgtata tcgattcgcc cgacgggctc gtcttccaac
     4141 tcgatcaaaa catgaatatt aaatggataa agaccattgg gggaacattg acagaccagg
     4201 tggaaggcct ggttgtttca gaagagggaa atattttcat cctttcggat cattacgcca
     4261 acaacgattt cccttgctat attgatttct ttcaacagga tactctgtta gcagaccgaa
     4321 actttatttc tgtttccggt ttgaattctg atggaatcct gcaatggaga aaagaatttt
     4381 acggtagttt ttctcctgca tacctgatga tgggtaaaga ttcctgcctt tacttctggg
     4441 gtgaagccag ttcggattat atcatcattg aaaatgatac tattgtcaaa cccggcaata
     4501 caactgtaga gcctgtcttt ttttcaagcc ttggtactaa cgggataata cagcacatgg
     4561 agtttgtaga gtttccggtc agaccgttgg aacttgatgt aagcatgtcg ggcgatatct
     4621 gtttttcggg caacgtttat gattcgatta ttattgatgg cgacagcatc aatgttcctc
     4681 aaggcactca ttatgccttt gtcgggaaat tcaatccgca ttatcaaccg gtttggtatc
     4741 aggttattca gcagactgcg ggcaaccaac tgggcgtatt tgatattgaa gcatgtggcg
     4801 aagacctggc ttttagctcc tataccaacc aggacgtgca aattgtcgac acggttttca
     4861 tcatcaatta tggaattgaa tcctttacag gattttttga taaagatgga aacctctcgg
     4921 aagtgaccat gaatgaaaca tcgggcgagt tctataccta tgagttggcc ctggacccat
     4981 gcaataatgt cctcctgtcc ggccagatgt caggaaaaat atacctgtac g
//
LOCUS       DAOQXS010000177         5024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_16952_length_5024_cov_3.685047, whole genome shotgun sequence.
ACCESSION   DAOQXS010000177 DAOQXS010000000
VERSION     DAOQXS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16952_length_5024_cov_3.685047"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKM34_06115"
     CDS             complement(<1..519)
                     /locus_tag="PKM34_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNQ83198.1"
                     /translation="MVDTVKIGTLKWHHIVSPSEHDLQYLKDNFHFHPLDIEDCRSTN
                     QRPKIDIYDDYYFIILHFPSFDLHNRFLTIKEVKFFWGEDYVISIDQTNWIVTEMFND
                     ARKLIASKEEIEIGTSDALLYRILEKLMNSSLNLMRRLGLELDRINEDLFSIRAQKTI
                     MQISITRKNIILV"
     gene            complement(691..3057)
                     /locus_tag="PKM34_06120"
     CDS             complement(691..3057)
                     /locus_tag="PKM34_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNQ83199.1"
                     /translation="MKKLTFTLLLLLSVFIVFPQAQYSRVKLFASREEIELLVKEGVG
                     IDHGIFRPGAYTICELSDAELSTVRESGISYEVIIEDVEKFYRERNEPFMDRLSELKE
                     AEYLLSRDWPIPDGFELGSCGGFLTIDQCMEHLDNMAVLYPDLITQRAPLSEMTHNNR
                     ELYWVRLSDNPGTDEEDEPEVLYTGMHHAREGIGMQLLVYYMYYLLENYETDPEVQYI
                     VDNFELYFVPIINPDGYAFNIQNQPAGGGMWRKNRRQNDDGSYGVDVNRNYGYMWGYD
                     NNGSSGEPSDDTYRGPDPFSEPEALNMKYFCEDHDFRIALNYHSYSNLFLYPWGWTEV
                     PCDHDAIFHAFGEIMTKENGYVFGAGSTTIYPTNGGSDDWMYGEQETKSLIYSYTPEV
                     GDGSAGFWPPVSQIIPLCQENMYANIMAARLSGPYATARDLSPSIIEYKTGLIFFEIQ
                     RLGLEDGATYTVSVEPLNDAFASISGPFTYTDLEILETESAAFTYTLREDILDGDAVE
                     FLLSVNNGLATFSDTIRKIYGTPVIIFEDDAESFTKWTSNKWNITTAQYHSPERSIAD
                     SPFGQYTNNENNIVTLVESIDLSDVVYASLGFWAKWDIEADYDYVQVLISDNNGGSWT
                     PVQGKFTRPGSSNQELGEPLYDGVKEDWVREEINLSAYLDKQIKIRFVLRSDTYVTGD
                     GFFWDDMTVTVVDQATGISSDSDLPKEQLISIQPNPSSETVTITLFSDKIDRLMIYNS
                     LGVAVHKVRVAEGDTEIKLDVSGWPSGIYFNALESGSSIVAIEKLVVR"
     gene            complement(3143..3724)
                     /gene="gldD"
                     /locus_tag="PKM34_06125"
     CDS             complement(3143..3724)
                     /gene="gldD"
                     /locus_tag="PKM34_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNQ83200.1"
                     /translation="MKFFLRLTIYNLLIVTFMAGCGEDYTPKPRGYFRIDMPDKEYTR
                     FDSTFPYSFEYPVYAKIVPDTRTTSEPFWMNIDFPQFNGRIHISYKPVEENLGEYLED
                     SRTFVIKHIPKADAIDDSLIYRPESAVYGLVYYIEGPAAASPCQFFITDSTSRFLRGA
                     LYFNFPPNNDSLAPVIDFIENDIRHLITTFRWK"
     gene            complement(3731..>5024)
                     /gene="gldE"
                     /locus_tag="PKM34_06130"
     CDS             complement(3731..>5024)
                     /gene="gldE"
                     /locus_tag="PKM34_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNQ83201.1"
                     /translation="SGIAAILLLLIGSAMASGAEVAFFSLGPNQIRELRTHGTRANQA
                     VLLLLEKPKRLLATLLIVNNFVNVSIVIISTYLTGILFNFRESSYWVAFLVQVVAVTS
                     LILLFGEILPRVYATRNNLKFARFLAGPVGLASKIFYPLSSMLIGSTKLIDKRISRKA
                     YNVSINELSDAIELATGADTPQEEKKILRGIVRLGDIEVKEIMKSRVDVTAVDISTGY
                     LELLQKIVGSGYSRIPVYGDSIDQVRGILYIKDLLPHLQEGEDFDWKGLLRDALFVPE
                     NKKINDLLQEFQERKIHMAVVVDEYGGMSGIVTLEDIIEEIVGEISDEHDEPLETFEF
                     TQIDDHHYIFEGKTSLNDFCKILDIGDEVFDDVRGESDSLAGLLLELLQRMPAKNEQV
                     SYRQFTFTVKAVDKRRIKRVMVSIDISGSFSDQKTTEN"
BASE COUNT         1304 a         1216 c         1198 g         1306 t
ORIGIN      
        1 gaccagaatg atgtttttcc gtgtgatgga gatctgcata atggttttct gggcccggat
       61 actgaaaagg tcttcgttga tccggtcgag ctccaggccc agccgccgca tcaggttgag
      121 cgatgaattc atcagctttt caaggatacg gtaaagcagc gcatcggagg tcccgatctc
      181 aatctcttcc ttgctggcaa tcagtttgcg ggcatcgttg aacatctcgg tcactatcca
      241 gttcgtctgg tcgatgctga tcacgtagtc ttcaccccag aagaatttca cctctttgat
      301 ggtcaggaaa cggttatgca ggtcgaagct gggaaaatga agaatgatga aataatagtc
      361 gtcatagata tcgatcttgg ggcgctggtt ggtacttcgg cagtcttcaa tgtccagggg
      421 atggaaatgg aagttgtctt tcaggtactg caggtcgtgc tccgacgggc ttacgatgtg
      481 gtgccacttc agggtaccga tttttaccgt atcaaccata tctgctccgg ttttataaga
      541 tgaatactat gcgaattgtt ctacatgcct gtaaaattta aaagcggtgc aaagataaac
      601 aaaggttccg gaaaggattg actttttcgg agaaaaatga atggttcagg aaaacgtgtt
      661 ttccggcgga ttcacaagtt gagccgccaa ttacctgacg accagttttt ctatggccac
      721 aatactgctt cccgattcaa gagcgttgaa ataaatgcct gaaggccagc cgctaacatc
      781 aagctttatc tccgtatctc cttcagcaac gcgtacttta tgaacggcca cacccagcga
      841 attataaatc atcagtctgt cgatcttatc agaaaacagg gtgatggtca ctgtttctga
      901 tgatggattc ggctggatgc tgatgagttg ttcttttggc agatctgaat cggaactgat
      961 gccggttgcc tggtcgacca cagttaccgt catatcgtcc cagaagaaac catcgccagt
     1021 cacatacgta tcgcttcgca atacaaaccg tatcttaatc tgtttatcga ggtaagcgct
     1081 caggtttatc tcttccctga cccaatcttc tttcaccccg tcgtaaaggg gttcgcccag
     1141 ttcctggttg gaactgccgg ggcgggtgaa ctttccctgg accggagtcc atgatcctcc
     1201 gttattatcg gagatcagta cctgaacata atcgtaatcc gcttcgatgt cccatttggc
     1261 ccagaacccc aggctggcat atacaacatc cgacagatca atggattcca caagggtaac
     1321 gatattattt tcattgttgg tgtactgacc aaacggggag tcggcaattg agcgttccgg
     1381 agaatggtac tgggctgtag tgatgttcca cttgttggag gtccatttgg taaaagattc
     1441 cgcatcatct tcgaaaatga tcaccggtgt gccatagatc ttccggatcg tatcggagaa
     1501 agttgccagg ccgttattta ccgaaaggag gaattcgacg gcatctccgt ccaggatatc
     1561 ttcgcgcagt gtataagtga atgccgcgct ttctgtttca aggatctcca gatcggtata
     1621 cgtaaacggc ccactgatgg atgcaaaggc atcattgagc ggttctaccg aaacggtgta
     1681 ggttgcaccg tcttccaggc ccagtcgttg aatctcaaag aagatcaggc cggttttata
     1741 ttcaatgatt gatggagaaa gatcgcgggc agtggcatag ggccctgaca gccttgccgc
     1801 catgatattg gcatacatgt tctcctggca caacggtatg atctgcgaca cgggcggcca
     1861 gaaaccggca cttccatcgc caacctcagg tgtgtaggaa taaatgaggg attttgtctc
     1921 ctgctcaccg tacatccagt cgtcggaccc gccattggtg ggatagatcg tggtgctgcc
     1981 ggcgccgaac acgtacccgt tctcttttgt catgatttca ccgaaggcat gaaagatggc
     2041 atcgtgatcg catggcactt ccgtccaacc ccatggataa agaaaaagat tgctgtacga
     2101 atgataattc aatgctatcc tgaaatcatg gtcttcacag aaatacttca tattcaacgc
     2161 ctcaggctcc gagaaaggat caggcccgcg gtatgtgtca tcggaaggtt cacctgatga
     2221 gccgttattg tcgtaacccc acatataacc gtagttcctg ttcacatcca cgccgtaaga
     2281 gccatcgtca ttctgcctcc ggttctttct ccacatacct ccgccggccg gctggttctg
     2341 aatattgaaa gcataaccgt ccggattgat gatcggaaca aaataaagct caaaattatc
     2401 gacaatatat tgcacctccg gatcggtttc ataattctcc agaagataat acatataata
     2461 aacgagcaac tgcatcccta tgccttcgcg ggcatggtgc ataccggtat ataaaacctc
     2521 cggctcgtct tcttcatccg taccgggatt atcggagagc ctgacccagt atagttcgcg
     2581 gttattgtgc gtcatctcgc tgagaggggc ccgctgtgta ataagatccg gatacaaaac
     2641 agccatattg tccagatgct ccatgcattg gtcaatggtg agaaaacctc cgcatgagcc
     2701 cagttcaaaa ccatccggaa tcggccagtc gcggctgagg agatattctg cctccttcag
     2761 ttcgctgagc ctgtccataa aaggctcgtt tctctcacgg taaaattttt ccacatcttc
     2821 aataattacc tcatatgaaa ttccagattc ccttacagtg gaaagttcgg catccgacag
     2881 ctcacagatc gtgtaggctc cgggcctgaa aatgccatgg tcaatcccca ctccttcttt
     2941 tacaagcaat tcgatctctt cccgggaggc aaataatttc acacgggaat actgagcctg
     3001 ggggaatacg ataaaaaccg acaataacaa cagcagggta aatgttaatt tcttcatctg
     3061 gtaagatatt tgagtcatca ggaatattat atgatcgcaa atataattat ttataaaaac
     3121 aagagaatga caagcccgga tttcatttcc acctgaatgt tgtgatcagg tggcggatat
     3181 cattttcaat aaaatcgatg acaggagcca gggagtcgtt gtttggcggg aaattgaagt
     3241 acaatgcccc gcgcaggaag cgggaggtgc tatcggtaat aaaaaactgg cagggcgagg
     3301 cggcagccgg gccttcgatg taataaacca ggccgtaaac agccgattcg ggcctgtaga
     3361 tgagtgaatc atcgatagcg tcggccttgg gtatatgttt gatcacgaat gtgcgggaat
     3421 cctcaaggta ttctcccagg ttttcctcaa ccggtttgta agagatatga atcctgccgt
     3481 tgaattgcgg gaaatcgata ttcatccaga agggttcgga ggtagtccgg gtatcgggaa
     3541 caattttggc ataaacgggg tattcaaaag aataagggaa tgttgaatca aaccgggtat
     3601 actccttatc aggcatatct attctgaaat accctcttgg ctttggggtg taatcctccc
     3661 cgcaacctgc catgaaggtc acaatcagca aattataaat tgttaatcgg agaaaaaact
     3721 tcatcttatg ttaattttcg gtagtttttt ggtcagagaa cgatccggaa atatcgatac
     3781 tgaccattac ccttttgatc ctgcgcttgt cgacagcctt aacagtaaaa gtgaactgcc
     3841 ggtacgacac ctgttcattt ttcgcgggca ttctctgcaa taactccagc agcaacccgg
     3901 ccagtgaatc cgactcaccc ctcacgtcgt caaatacttc atcgccgata tcgagaatct
     3961 tgcagaagtc gttcagcgaa gtctttcctt cgaaaatata atgatgatca tcgatttggg
     4021 taaactcgaa ggtttccagg ggctcatcat gctcatcact gatctcccct acgatctcct
     4081 cgatgatgtc ttccagggtt acaatcccgc tcatgccgcc gtattcatcg accaccacgg
     4141 ccatgtgtat ctttctctcc tggaattcct gcagcaggtc gttgatcttt ttattttcgg
     4201 gcacaaataa agcatcacgg agcaaacctt tccagtcgaa atcctcgccc tcctgaagat
     4261 gtggcaacag gtccttgata taaagaatcc cccttacctg gtcgatcgag tcgccataaa
     4321 ccgggatcct ggaatagcct gacccgacta ttttttgaag gagttcgaga tagcccgtcg
     4381 aaatatcgac ggcggtgaca tcgacccgtg atttcatgat ctccttcacc tcgatatcac
     4441 cgagccggac gataccccgg aggattttct tctcctcttg cggcgtatcg gctcctgtgg
     4501 cgagctcaat ggcgtcggaa agttcgttta ttgaaacatt ataagccttc cggctgatcc
     4561 tcttatcgat cagtttggtt gagccgatca gcatggagct gagcggatag aagattttcg
     4621 acgccaggcc gaccgggcca gccaggaacc gtgcaaattt caggttgttg cgtgttgcgt
     4681 aaactctcgg aagaatctca ccgaacagca aaatcagcga ggtcactgcc acaacctgca
     4741 ccaggaaagc gacccaatac gacgattccc ggaagttgaa caggatgccg gtcagatagg
     4801 tcgagataat gacaatagaa acattaacga aattattgac aatcagcaat gtagctaata
     4861 atcttttcgg tttttcgagg aggagcagga ccgcctggtt ggctctggtg ccatgggttc
     4921 taagctcacg gatctggttc gggccaaggg aaaaaaaagc cacctcggcg cccgaggcca
     4981 tggctgaacc gatcagcagc aacaaaattg cagcgattcc ggag
//
LOCUS       DAOQXS010000178         5001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17064_length_5001_cov_3.932673, whole genome shotgun sequence.
ACCESSION   DAOQXS010000178 DAOQXS010000000
VERSION     DAOQXS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17064_length_5001_cov_3.932673"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="mnmA"
                     /locus_tag="PKM34_06135"
     CDS             complement(<1..767)
                     /gene="mnmA"
                     /locus_tag="PKM34_06135"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ83202.1"
                     /translation="MQIAALVSGGVDSSVIVPLLKEQGYDPVIFYIQIGMENEPGFMD
                     CPSEEDIEITTFIAKKYGCRFEIANLHEEYWNRVVNYTIESVSKGLTPNPDVMCNRFI
                     KFGSFEDKFGKDFDKIATGHYAQVLEKEGQFFLGTSKDRRKDQTYFLGRITYPQLSKA
                     MFPIGGFEKGEVRAMAERLKLPSAGRPDSQGICFLGKVNYNEFIRRYTGEKEGDIVEL
                     ETGKILGKHKGYWFHTIGQRKGLGLSQGPWFVVKKDIP"
     gene            complement(858..1820)
                     /locus_tag="PKM34_06140"
     CDS             complement(858..1820)
                     /locus_tag="PKM34_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ83203.1"
                     /translation="MKNILVIGSVGQIGSELTMRLREVYGASHVVAGYRKTMPPKELA
                     ESGPLEMVDATDAAKMAEVVKKYKIDTIYNLAALLSAVAEGNPTMAWNVGVGGVFNCL
                     EVARENNCAIFFPSSIGAFGPSTPQDNTPQDTIQRPSTMYGVTKVTGELLSDYYFKKF
                     GVDARGLRYPGIISNVTLPGGGTTDYAVEIYYAAIKEKKFTCPLPAGTFLDMMYMPDA
                     LDAAIDLMEADPSKLKHRNAFNVSAMSFDPEIIAGEIKKHIPDFVMNYNVDPMKKAIA
                     DSWPNSMDDSCARAEWGWNPKWKLDNMTVDMLKVIGEKHAKGLI"
     gene            1928..2419
                     /locus_tag="PKM34_06145"
     CDS             1928..2419
                     /locus_tag="PKM34_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNQ83204.1"
                     /translation="MNIRFTEITEADFAVIKGIYDYYILNSTSTYYTELISIDELKQF
                     IPLNSQRYKSYMIEAGGECCGFCYLSQYKKRQAYDRTAEISLYLKNGYTGRGIGRIAL
                     DYLEEIAREKGIHVLLAIISGDNIASIKLFERAGYEKCGHLKQIGEKFHRVLDVLFYQ
                     RFL"
     gene            complement(2461..3684)
                     /locus_tag="PKM34_06150"
     CDS             complement(2461..3684)
                     /locus_tag="PKM34_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83205.1"
                     /translation="MPSFHRNKPVLPLYLIIILLSLLGLKPGKTFAQEENDSPVNYIN
                     SMEYAFMMHEDTRWLLKADLVVKNEYVNMNPFKISFEHRIYKSLTANASIDHVNMNMP
                     GDHMYFLPLNFSIEPRWYYRLSKRMKDGNAASSLSDNYLAMGVSYTWLLNYNQVESPV
                     DRRYVSYYAKWGMQRRYLKYGHIDMGIKAGMMDAAGNGFSPSLVLNTFVEMGLGFSKD
                     RQAMDRERLCPFLRCYEADRFIVKTNLTDLVNLGIFMDQKWVELSPQVALEHKLGTSS
                     FSLNASIGGTLGYAEFSEADKVLTDRYWIGEVSLEGRYYYNLKKSILNGLSGNGLSAN
                     YLAAGGGYCVTDGQYDEMIKETGPRLYLATGWQRLLNRHLYFDIQVGANYNFRPNRAG
                     NVYEPMFQYGLGYRF"
     gene            complement(3800..4582)
                     /locus_tag="PKM34_06155"
     CDS             complement(3800..4582)
                     /locus_tag="PKM34_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83206.1"
                     /translation="MKRGILYMLFFFILAGNSFPQVRVQVVSREINKTIAWKEGMSLV
                     VNGDNAEIYCSSGFHQDIGISMTIISKHEERTVAEADLEKMKWLVEVKDNTCFVRNYI
                     ELSRNQARPESALKVILRITVPADCPVSLHNYFGKTEISGLNSEVTLNNEYGPVLLSD
                     LNGNTTVNSVFGDVTINGSMGHTRITSTHSQIVLRLNALYQYGFNFELTDSEIIKPEE
                     MIIIYDKKEKGKITGQIKGQNSGPEISVILKNCSLTIEQSNN"
     gene            complement(4584..>5001)
                     /locus_tag="PKM34_06160"
     CDS             complement(4584..>5001)
                     /locus_tag="PKM34_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83207.1"
                     /translation="ALKDRGKGEKRSIAILKWTAAVAAIVIPFVIIFSVTGDREIRTH
                     YSEEWVVMADIRNNTSDDSLSYHVIDNKCRKKPEICQSDEFLGLKKELAFLDNSKKTI
                     INRLNKYESGKDLEALLIRIDLEREEILGKLIAISN"
BASE COUNT         1213 a         1209 c         1204 g         1375 t
ORIGIN      
        1 gggatatcct ttttaacgac gaaccacggt ccctggctaa gccccagtcc ttttcgctgg
       61 ccgatggtat gaaaccagta gcctttgtgt tttccgagga tcttcccggt ctccagctcc
      121 acgatatccc cttctttttc accggtataa cgccggatga attcgttgta gttgaccttt
      181 cccaggaagc atatcccctg gctgtcggga cggcctgcgc tgggcagttt cagcctttca
      241 gccatcgccc tcacctcccc tttttcgaac cctccaatcg ggaacatggc ttttgaaagc
      301 tgaggatagg ttattctccc caggaaatag gtctggtctt tccgccggtc tttggaggtc
      361 ccgaggaaaa actgcccctc tttttccaaa acctgtgcat agtgtcccgt cgcgatttta
      421 tcaaagtcct tcccgaattt atcctcgaag ctgccgaact tgatgaagcg gttgcacatc
      481 acatccgggt tcggcgtcag ccccttgctg acagactcga tcgtgtaatt caccacccgg
      541 ttccagtact cttcgtgcag gttggcgatt tcgaagcggc agccgtattt ttttgcgata
      601 aacgtagtga tctcaatgtc ttcttccgac gggcagtcca taaatcccgg ctcattctcc
      661 atcccgatct ggatatagaa aataaccgga tcatagccct gctctttaag caaaggaacg
      721 atcacggagc tgtcgactcc accggaaacg agggcggcga tttgcatggg gcaaaggtag
      781 ggaaaatcag ggagatccct cacttcgttc gggatgacgt atcatcccga acagagcgaa
      841 ggaacttaaa gaagttgtca gatcaaccct ttcgcgtgtt tttccccaat caccttcagc
      901 atatcgaccg tcatgttgtc gagcttccat ttcgggttcc agccccactc ggcgcgggcg
      961 cagctgtcgt ccatgctgtt cggccatgaa tcggcgatag ctttcttcat cggatcaaca
     1021 ttatagttca tcacgaaatc gggaatatgc tttttgatct cgccggcgat gatctccgga
     1081 tcgaagctca tagcgctgac gttgaacgca ttgcggtgct tcagctttga cggatcggct
     1141 tccatcaggt caatagctgc atcaagcgca tcgggcatgt acatcatgtc gaggaaggtg
     1201 ccggccggaa gcgggcaggt gaatttcttc tccttgatgg cagcgtaata gatctcaacg
     1261 gcgtagtcgg tagttccgcc gccggggagg gttacgttgg agataatgcc gggatagcgg
     1321 aggccgcggg catcgacgcc gaatttcttg aagtaatagt cgctgagcag ctcgccggtc
     1381 actttcgtca caccgtacat ggtactgggg cgctggatgg tatcctgcgg ggtgttatcc
     1441 tgcggggtgg aggggccgaa agcgccgatg gagctcggga agaagatggc gcagttgttt
     1501 tcacgggcca cttcgaggca gttaaataca ccgccgacac ccacattcca tgccatggta
     1561 gggttgcctt ccgctacagc ggataagagg gcggcaaggt tgtaaatagt atcgatctta
     1621 tattttttca cgacttcggc cattttcgca gcgtcggtcg catccaccat ttccagcggg
     1681 ccggattctg ctaattcttt ggggggcatg gttttacgat agccggcgac gacatgcgaa
     1741 gcgccataaa cttcccttaa acgcatggtc agttccgatc cgatctgacc cacagagccg
     1801 ataacgagta tgtttttcat tttgatctag tttttgtgaa ataattaaca atttgcggca
     1861 aaagtaagaa aattgtgata tgtggtaatt tgaattaaat ttattgcttt aaacgatgat
     1921 cccggttatg aatatccgat tcaccgaaat aacagaagcc gattttgcgg tcataaaggg
     1981 aatctatgat tattatattc tgaattcgac ctcgacctat tacacagaac tgatttcgat
     2041 cgatgagctg aaacagttca ttccactgaa tagccagaga tataaatcgt acatgatcga
     2101 agccggtggg gaatgctgcg gattctgtta cctttcgcag tataagaaga ggcaggctta
     2161 cgacaggacc gccgaaatct cactttattt gaaaaacgga tatacgggaa gaggaatcgg
     2221 gagaatagca ttggattacc tggaagaaat tgcccgggag aaagggatac acgttttgct
     2281 tgccatcatt tccggagata atatagcgag tataaagttg tttgaaagag ccggatatga
     2341 aaagtgcggc cacctgaaac agatcgggga gaagttccac cgggtgctgg atgtgttgtt
     2401 ctaccagcga ttcctgtgac aataaaccgg tgtatatgcg ataagaccga tcagttcatg
     2461 ctagaacctg taccccaggc cgtattggaa catgggctca taaacatttc ccgcgcggtt
     2521 tggcctgaaa ttgtaattgg ccccgacctg gatatcgaag tacaaatgcc ggttgagcag
     2581 ccgttgccac cctgttgcca ggtaaagccg ggggccggtt tctttgatca tttcatcata
     2641 ctggccatcg gtcacgcaat aacctcctcc tgcagccagg tagttggccg acaacccgtt
     2701 acccgacagg ccgttgagga tgcttttctt caggttatag taatatcttc cctctaagct
     2761 tacttcgcca atccagtacc ggtcggtcaa tactttgtcc gcctcactga actcagcata
     2821 gcccaaagtc ccaccgattg aggcattcag tgaaaatgac gatgttccga gcttgtgctc
     2881 caatgcaacc tgcggagaaa gttccaccca tttctgatcc atgaaaatgc cgagattgac
     2941 caggtcggtc aggttcgttt tgacaatgaa ccggtcggct tcatagcatc tcaggaacgg
     3001 gcacagcctt tccctgtcca ttgcctgcct gtctttcgaa aagcccagcc ccatctccac
     3061 aaaagtattc agaaccaggg aaggggagaa gccgttgccc gctgcatcca tcatccccgc
     3121 tttgatgccc atgtcgatat gcccgtactt caggtaacgg cgctgcatcc cccatttggc
     3181 gtagtacgaa acataccggc ggtcgaccgg cgattccacc tggttataat tcagcaacca
     3241 tgtgtacgac acacccatgg cgagatagtt gtccgacagg ctggaggccg catttccatc
     3301 tttcatccgc ttgctcaacc ggtaatacca gcggggttca atggaaaaat tcaacggcag
     3361 aaaatacata tgatcgccag gcatgttcat gttgacatga tcaatggaag cattagccgt
     3421 caaactttta taaatcctgt gttcaaatga tattttgaac ggattcatgt tcacatattc
     3481 atttttcacc accaggtcgg cctttaacag ccaccgggta tcttcatgca tcatgaaagc
     3541 gtattccatg gaattaatat aatttactgg gctgtcattt tcttcctggg cgaaagtctt
     3601 acccggtttc aaccccagga gtgacaggag aataatgatc aggtacaggg gaaggacagg
     3661 cttatttcgg tgaaatgacg gcattgttaa agtgttaaag tgttaaaggg ttaaattgtt
     3721 attgtgttcg attgttcaat tgttcgattg ttcaattgtt cgattgttcg attgttcgat
     3781 tgttcaattg ttcgattgtt caattgttcg attgttcgat tgttaaggag cagtttttca
     3841 ggataacaga tatttcgggg ccggaatttt gccccttgat ttgtcccgtg atctttcctt
     3901 tctctttttt atcataaatg atgatcattt cttctggttt gataatctca gaatccgtta
     3961 attcgaagtt aaagccgtat tgataaagag cattcagacg gagaacgatc tgtgaatggg
     4021 tggaagtaat tcgggtatgg cccatggagc catttatggt aacatctcca aagaccgaat
     4081 tcaccgtagt attcccattc aggtctgaca aaagcaccgg cccgtattcg ttgtttaggg
     4141 tcacttctga atttaaaccg ctgatttcgg tctttccgaa atagttatgg agactgaccg
     4201 ggcaatcggc cgggacggtg atccggagga tgactttcaa ggccgactcg ggccgggcct
     4261 ggttccgcga gagttcgatg taatttctga caaagcaggt gttgtctttc acttcaacca
     4321 gccatttcat cttttcaagg tcggcttctg cgactgtccg ttcttcatgt ttggaaataa
     4381 tagtcatgct gatcccgata tcctgatgaa agcctgaaga gcagtatatt tcagcattat
     4441 ccccattgac gaccagactc atcccctctt tccaggcgat cgttttattg atttccctgg
     4501 aaacaacctg caccctgact tgcggaaatg agtttccagc caggatgaag aaaaagagca
     4561 tgtatagaat tcctcgtttc atctcaattg gaaatagcga tcaatttgcc caggatttct
     4621 tcccgttcca ggtcgatacg gatcagcagg gcttcgagat ctttgcccga ttcatattta
     4681 ttcaggcggt ttataatggt tttctttgaa ttatcaagaa aagcaagctc cttttttaag
     4741 ccgagaaatt catcagactg gcagatttcg ggctttttcc ggcatttatt gtcaatgacg
     4801 tggtaagaca gggaatcatc acttgtgtta ttgcggatat cggccatcac gacccattcc
     4861 tctgaataat gggtcctgat ttccctgtcg cccgtcacac tgaaaatgat aacgaaagga
     4921 atgacaattg ccgcgaccgc agcggtccat ttcaggatgg caatactccg tttttcccct
     4981 ttaccccgat cttttaaggc a
//
LOCUS       DAOQXS010000179         4974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17195_length_4974_cov_3.558853, whole genome shotgun sequence.
ACCESSION   DAOQXS010000179 DAOQXS010000000
VERSION     DAOQXS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17195_length_4974_cov_3.558853"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKM34_06165"
     CDS             complement(<1..1024)
                     /locus_tag="PKM34_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83208.1"
                     /translation="MKKNVIIIGAAGRDFHNFNTYFRGNESFNVVAFTAAQIPDIDGR
                     KYPAELAGHLYPDGIPIFAESNLPELIKELKVDICVFSYSDVTYQKVMAISAIVNSAG
                     ANFLLLGPRDTMVKSTKPVIAVGAVRTGCGKSQTSRRIIEILMAKGLKVVAVRHPMPY
                     GNLVEQKVQRFATIADLAKHKCTVEEMEEYEPHVVRGNVIYAGVDYEAILRAAEQDPD
                     GCDVVLWDGGNNDFPFYTPDLNFTVVDPHRPGHELTYYPGEVTLRLADVVIINKIETA
                     EPKSIQVVRENIAKVNPKAIVVDGASPIKVDNPSVIKGKRVLVIEDGPTLTHGEMKIG
                     AGTVAAQ"
     gene            1147..2937
                     /gene="argS"
                     /locus_tag="PKM34_06170"
     CDS             1147..2937
                     /gene="argS"
                     /locus_tag="PKM34_06170"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ83209.1"
                     /translation="MIEKIIAEKASDALKSLYGQNIDPKGVAIQKTRPEFQGDFTVVV
                     FPLTRFSGKSPEETGRELGEWLKGNFNFIADFNIIKGFLNILLTDRYWMDFFSAGSLD
                     ENFGFNPQSEGPPVVIEYSSPNTNKPLHLGHIRNNLLGWSIAEILKANGRKVMKVNLV
                     NDRGIHICKTMLAYRKWASGKIPSKEGIKGDKFVGNLYVQFEQANKKIMETIIKQGID
                     EDEAYRQSGLTQEALDLLQQWESGDPDARRLWETMNGWVYEGFDQTYRRLGVDFDKIY
                     YESETYLLGKDLVAEGLDKGVFYKKDDGSVWIDLKDFKLDEKLLLRPDGTSVYITQDL
                     GTAQLRVDDYHPGRLLYVVGNEQIYHFDVLKIVLKLLGRDWAENILHVSYGMVELPQG
                     KMKSREGTVVDADELMEEMYETAKKTTAELGKLDNPDDPEWRELFEMLGMGALKFFIL
                     RVDPRKNMLFNPQESIDFNGHTGPFIQYTHARIRSLLAKASGKGYKAITSPLPDDFAL
                     LPKERAIVRLLYDFPAVVADAGNNLSPAIIAAYCYELAKEYNQFYQEIPVLKEPDPLK
                     ISFRLALSGFAGNVLRRGMGLLGIRVPEKM"
     gene            complement(2946..3890)
                     /locus_tag="PKM34_06175"
     CDS             complement(2946..3890)
                     /locus_tag="PKM34_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNQ83210.1"
                     /translation="MFKRFSSVILMAILAGLLIVYFIVRFSGTNDRTFRDKVLSFDPA
                     VVTSITIDDPKSMQEPVELRLSGEKWMVRSGVKEYPADSNIVKGALNTLSDMPTKRFA
                     GKGKESWIKYEVTDTSGIKVTLKSSSKTVAEILIGKFTFNMPKDQQQSQFRQQRGDMT
                     SFVRLADEKDVYAVDGYLRTTFSAKPDSYRYKNLSGVNAQDITKMTVSKPGGLTVYDL
                     STGKWTMNGMPADSATTARYRSAVARLSGSKFVDPEMVRSAPAYTLRLEGNNFSPVDI
                     MAYPVADTNIAYAISSSVNPDAYFDGKTGGLFKRIWPD"
     gene            complement(3883..>4974)
                     /locus_tag="PKM34_06180"
     CDS             complement(3883..>4974)
                     /locus_tag="PKM34_06180"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNQ83211.1"
                     /translation="SEKEMIPMVQAGSAMEYDLASSIKKLSVTDKPAIGLIQGHGEPS
                     MGALQQVMASLGILYSVRPVDLENPSDDPGKYATIMMVAPTDTIPAHHFDVLDRYLAG
                     GGNLFIAYNKVKGDFTTVQGTSNYTGLEDWLAKKGLYIDNNFLVDATCGSVGVQQQAG
                     FMSFTTNVSFPYLPVIQKFADHPVTKGLEQVMLPFASTIQFTGDTSLAFTPIAFSSQK
                     SGTLNPPLYFDINKKWTDRDFPLSGLTVAGILSGRMGGMAESKIILVGDGDFPVNGEG
                     QRPQQLQPDNVNLMVNAVDWLSDETGLIDLRTKAITARPLKDVAEGRALFIKWLNFLL
                     PIILIILYGIFRNQRNRSLRVKRMEEGYV"
BASE COUNT         1234 a         1157 c         1256 g         1327 t
ORIGIN      
        1 tctgtgcggc aacggttccg gcgcctattt tcatttcacc gtgggtaagg gtggggccgt
       61 cttcgataac caggaccctt tttcctttga tcactgaggg attatccact ttgatcggtg
      121 atgcgccatc caccacaatg gctttgggat tgacttttgc aatattttcc ctgaccacct
      181 ggatgctttt gggctcggcg gtttcaattt tattgatgat gaccacatcg gccaggcgca
      241 gggtcacttc tccagggtaa tatgtcagtt catggcccgg acggtgggga tccaccacgg
      301 tgaagttaag gtccggggtg tagaagggga aatcattgtt tccaccatcc cagagcacga
      361 cgtcgcatcc atcgggatcc tgttcggctg cgcggagaat ggcttcataa tcgacgccgg
      421 catagatcac atttcccctg accacatggg gttcatattc ttccatctct tcaacagtgc
      481 atttgtgttt ggccaggtcg gcgatggtgg caaaacgctg aactttttgt tccaccagat
      541 tgccataagg catgggatgc ctgacagcca caactttcag cccttttgcc atcaggatct
      601 cgatgatccg gcgggaggtc tggctctttc cgcaacccgt cctgactgca cccactgcga
      661 tcaccggctt ggtcgactta accatcgtgt ctctggggcc gaggagcagg aagttggctc
      721 cggctgaatt tactatggcg ctgattgcca tcactttctg ataggtgaca tcgctgtagg
      781 aaaagacaca gatatcaact ttcagctcct taataagttc cggtaagttt gattcagcaa
      841 agattgggat cccgtcagga taaaggtggc cggccagttc agccggatac ttccttccgt
      901 caatgtcagg gatctgggcg gctgtaaagg ctacaacatt gaacgattca ttgccgcgaa
      961 aataggtgtt gaaattgtga aagtccctgc ctgcagctcc gataataatc acatttttct
     1021 tcattccgta tgcttgttaa gttagtgata catattttgg taacaaagat agaattttaa
     1081 cccgaaccct gattggctcc tttctgaatt tttatttgcc tatttttgac aatctgaatt
     1141 tatacaatga ttgaaaaaat aattgctgaa aaggcttccg atgctttaaa atcgctttac
     1201 ggacaaaata ttgatcccaa aggagttgcg atccaaaaaa cgcgccctga attccagggt
     1261 gatttcaccg tggtcgtttt tccgctgaca aggttttcag gaaaatcacc ggaagaaacc
     1321 ggccgggaac tgggtgaatg gcttaaagga aattttaatt tcatagctga tttcaatatc
     1381 attaaaggat tcttgaatat ccttctcacc gatcgttact ggatggattt tttttccgcg
     1441 ggaagcctgg atgaaaattt cggatttaat ccccagagcg aagggccccc cgttgttatt
     1501 gaatattctt ccccaaatac caacaagccc cttcatctcg gtcatatccg gaacaatctt
     1561 ctgggatggt cgattgccga aatcctgaaa gccaacggac ggaaagtcat gaaagtcaac
     1621 ctggtgaacg acagggggat ccatatttgt aagaccatgc tggcttacag gaagtgggca
     1681 tcagggaaaa ttccttccaa agagggcatt aaaggggata agtttgtcgg taacctgtac
     1741 gtacaatttg agcaggccaa taaaaagata atggaaacca ttatcaaaca aggaattgac
     1801 gaggatgaag cataccggca atcgggcctg acgcaggaag cccttgatct tttgcaacaa
     1861 tgggaatcag gggaccccga tgcacgccgg ttatgggaaa caatgaacgg gtgggtttat
     1921 gaaggatttg accagaccta caggcggtta ggagttgatt tcgataaaat ttattatgaa
     1981 tccgaaacat accttctggg caaagatctg gtagcggaag gtttggataa gggtgtgttt
     2041 tataagaagg acgatggttc ggtgtggata gacctgaagg atttcaaact ggatgagaaa
     2101 cttcttttaa gaccggatgg gacttcggtt tatatcacac aggacctggg aacggctcag
     2161 ctccgcgtgg atgattatca tccggggcgt ctgctttatg tggttggcaa tgagcagatc
     2221 tatcattttg atgtcttgaa aatagtcttg aagctactgg gtcgtgactg ggcagagaat
     2281 atccttcatg tatcctacgg tatggtcgag ctgccgcagg gcaagatgaa atcgagggaa
     2341 gggacggtgg tggatgccga tgagttgatg gaagaaatgt atgaaacggc aaagaaaacc
     2401 acggctgaac tgggtaagct ggataatccc gatgatccgg aatggcgtga gctttttgag
     2461 atgctgggga tgggggcgct gaagttcttt atccttcgtg tcgatcccag aaagaacatg
     2521 cttttcaatc ctcaggaatc aattgatttt aatggccata ccggaccatt catacagtat
     2581 acgcatgcac ggatcagatc attactggcc aaggcttccg gtaaaggcta caaagccata
     2641 acttcaccgc ttccggatga ctttgcacta ttgccgaaag aaagggcgat tgtcaggctt
     2701 ttgtatgatt tcccggcagt ggtggccgat gcggggaaca acctcagccc tgccatcata
     2761 gccgcttatt gctatgaatt ggcaaaagag tataaccagt tctaccagga aataccggta
     2821 ctgaaagaac ccgaccccct gaagatttct ttccggctgg cgctttccgg gtttgccgga
     2881 aatgttttga gacgcggcat gggacttctt gggattcggg ttcccgaaaa aatgtaatgt
     2941 tcctgttaat cgggccagat tcgtttgaac aatccgccgg ttttaccatc gaaataggcg
     3001 tcaggattta ccgatgacga aatggcgtat gcgatattgg tatcggcaac aggataagcc
     3061 ataatatcga ccggggagaa attatttcct tcaaggcgca gggtataagc cggcgccgat
     3121 ctgaccatct ccggatcgac aaacttactt cccgacagtc gggctacggc cgaccggtac
     3181 ctggccgttg tagctgaatc ggccggcata ccgttcatag tccattttcc tgtagataga
     3241 tcataaacgg ttaaccctcc gggcttcgaa accgtcattt tagtaatatc ctgcgcgttg
     3301 actcccgaca ggtttttgta cctgtaagag tcgggtttgg ccgagaaagt agtccgaaga
     3361 tagccgtcaa cagcgtagac atctttttcg tctgccagcc tgacaaacga tgtcatatct
     3421 cctctttgct gccggaattg gctctgttgc tggtctttcg gcatattaaa cgtaaatttt
     3481 ccgattagga tctcagccac agttttggat gaagatttta atgttacttt gatgcctgag
     3541 gtgtctgtca cttcatattt gatccatgac tctttccctt taccggcaaa ccttttggtc
     3601 ggcatgtcgc tcagcgtatt cagcgcgccc ttcacgatat ttgaatcggc tgggtactcc
     3661 ttaacccctg atctgaccat ccatttttca cccgataacc ggagttcgac aggttcctgc
     3721 atgcttttgg gatcgtcgat cgttatggaa gtcacaactg ccggatcaaa ggagaggact
     3781 ttatccctga aagtgcggtc gttggtgccc gagaaccgga caataaaata tacaatcagc
     3841 aatccggcca ggatcgccat taagatgact gatgaaaatc ttttaaacat aaccttcctc
     3901 cattcttttt acacgtaaac ttctgtttct ctgatttctg aagatgccgt acagaatgat
     3961 caggatgatg ggaagaagga agttgagcca tttaatgaac agggcccttc cttcagcgac
     4021 atccttcaac ggccttgcgg taatagcttt ggttctgagg tcgatcagtc cagtctcatc
     4081 cgacagccag tcgacggcgt tgaccatcag gttgacatta tcgggctgca gttgctgggg
     4141 ccgctggcct tctccgttca ccgggaaatc gccgtcgcca accaggatga tcttcgattc
     4201 ggccattcct cccattcttc cggataaaat tccggctacc gtcagcccgg atagcggaaa
     4261 gtcgcgatca gtccattttt tattgatgtc gaaatacagg ggcgggttaa gtgtgcccga
     4321 tttctgcgat gaaaaagcga ttggggtgaa agccaggctg gtatcgccgg tgaactggat
     4381 cgtgctggca aaaggcagca tcacctgctc gagtcctttg gtgaccggat gatcggcaaa
     4441 tttctggata acaggaagat atgggaagct gacattggtc gtgaaactca taaaaccagc
     4501 ctgttgctga accccgacgg aaccgcaggt agcatccacc aggaaattat tatcgatgta
     4561 caaacctttt ttcgccagcc agtcttccag gccggtgtaa tttgatgtcc cctgaactgt
     4621 tgtaaaatcc ccttttactt tattataagc gatgaaaagg tttccgccac cggccaggta
     4681 acgatcgagc acgtcgaaat ggtgggccgg gatcgtgtcg gttggcgcaa ccatcatgat
     4741 ggtagcatac tttccgggat catccgaggg attctcaaga tctaccggcc ggacggagta
     4801 caatattccc agcgacgcca ttacttgctg cagtgcgccc atggaaggct cgccgtgccc
     4861 ctggatcagc ccgatggccg gtttgtcggt caccgaaagc ttcttgatcg aagaggccag
     4921 gtcatattcc attgcgcttc cggcctgtac catcgggatc atctcttttt cgct
//
LOCUS       DAOQXS010000180         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17199_length_4973_cov_3.459740, whole genome shotgun sequence.
ACCESSION   DAOQXS010000180 DAOQXS010000000
VERSION     DAOQXS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17199_length_4973_cov_3.459740"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4772)
                     /locus_tag="PKM34_06185"
     CDS             complement(<1..4772)
                     /locus_tag="PKM34_06185"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNQ83212.1"
                     /translation="MIGPGLSAGLASSRADGFISGYVFNYYGVAISGATIGKENGPFT
                     TSDNNGYYILDRLPEGEHDIGCGKPGYNFSWITVTVISADTTYQNFTLTQPEMVINPL
                     LIEQTLNPGEYITTYINILNTGTGSLHWESVIDYSAQSREINSEWLTMGSYQGDVEPF
                     GGLANVPVYLNATGSVSGQTYSAAIMFSSVPNVGNITVPVTMTVMGNELIPPENLEVS
                     LLDNVDGIVDLSWEWSGDAFQFFLIRRNGQIIATPTQTSFQDILENHGIFCYTVQAVY
                     HEGVTSPAGPACIEWPDPLLQINPVELEGAVWPGYEVDVFTTLSNQGEGTLSFSFPEF
                     AALALAGNSSVRGAGGPDDFGYVWIDNDEPEGPSFKFLDISTTGNPVFGISDDNVVGP
                     FPVGFDFPYYGENKTQFWINSNGAVGFTPNYITLGNTGIPTNSSVYRDFIAWLWDDLV
                     FITGTSQVFYQTVGNKLYIQFKNIQHFNQDDLYINAEIILNKNGKIIVIYEDIDEGVI
                     LNTCTVGLQSHDPEVGLQVAFNQEYLHDDLVILYSVPADFITQVTPASGTIPQGTSTV
                     VTITYDSKDYGPGAYSDDLLLFTNDIENDQMVLENTMYVYLPGIFSGVVTDMDNDEPL
                     NGVSVTAGPFQTTTGENGEYTLFVDEGRYDVVFGKLGYIPVTIYDTATAQGNITPVNT
                     GLWDMNYAPGFVSASVNAGETWCNVAWSLPNGPYEVIMDDGEADDFFVYAQAGSWSAV
                     KFMPTGYPALVLGGKFYVGDGSFPGPFLGTDFGVAVFDDDGVDGKPGTMLDSTGVTVN
                     NSGWVSLDGLNVLITEGSFYLAMYQAGNVPFAAPIGIDSDNPVQYRSYIQFQDNGWML
                     SPLQDFMIRAWISGPENDMKMPVPQLSLKAIPKIPSSWNKYAYTKSGNIPLLVPGYER
                     YEFELTGVNRPAVRDVVSYQVVRFSDFDPDLPPQTGNETILDTLTDLSYPDSAFGSLQ
                     QGWYAYGVRALFTSGLYSDFRISNTVGRMMDCTAGFSISLSTGEEPSGVEISMTGYDF
                     PYATITETTDPDGNAILEPVWKGHYDIRIYKIGYDPYLITDEYIEDDVSFEVVLSEKK
                     YPPTCLQVDSLSLMGNWCEPLFSLLDEDFESAVFPPSGWQNLTSSQAGGWMRGSNGSS
                     PNWTIPAHDSFYAYVNDDAAGSESDGCCDYLVTPALDLRESEGFVLSFDSYYDGFYGQ
                     LASVEYSTDGGGSWETLYNLPPDTSWHSMDVDLSAFSGQNGYSEIWLAFHADDGGEYA
                     SGWAVDNVRVGVPAPAAGYLDFAVFLDGALLGMTSQTSWDFAPLPYGQTYTASVAARY
                     SSGLSEKDYDTFRSVYLFPPKNLTGSAPDDAVILHWDPPGVVVPFNLLGYNIYKNGAL
                     LFYKDHEGGWEPQEYVEPEMQPGIYHYEVTGVYDLASYGFPGETGESMKEGPETVTVD
                     YCTELEFLETWEIESFNETSWNSDGDNWFVNGRAGNPVPCAEFSQVPVVTDYFLPLES
                     YPICAVGITEGEIWLTFDIAGYSVLNTGEEQMLVQVWNWESQIWSTVGEFSNIDGSFD
                     WTPGRINIRPYVMNSIFKIRFVARGINSANIRGWFLDN"
BASE COUNT         1371 a         1245 c         1149 g         1208 t
ORIGIN      
        1 atattatcga ggaaccagcc gcggatgtta gcggaattga tccctctggc tacaaatcgt
       61 attttaaaga tcgagttcat aacgtacggc ctgatattga tccggccggg agtccagtca
      121 aaggatccgt caatattgct gaattccccg acggttgacc atatctgtga ttcccagttc
      181 cagacctgaa ccagcatttg ttcctccccc gtattcagga ccgaataacc ggcaatatca
      241 aaagtcagcc agatttcgcc ctcggtaatg cccacagcac agatgggata cgattccagg
      301 ggcaggaaat aatccgtcac aacaggcacc tgggagaatt cagcgcaggg taccgggtta
      361 ccggcgcggc cattgacaaa ccagttatca ccgtcagaat tccagctggt ttcattgaaa
      421 ctttcaatct cccaggtttc caggaattca agttctgtac aataatcaac tgttacggtt
      481 tcagggcctt ctttcatcga ttcgcccgtc tcgcccggaa atccatagga agccaggtcg
      541 tagacaccgg ttacctcata gtgatagatt cccggttgca tttccggttc cacatattcc
      601 tgaggctccc agcctccttc atgatcttta taaaatagta aggcgccatt cttgtaaatg
      661 ttgtatccca gcagattgaa gggaacgaca actcccggcg ggtcccaatg gaggataacg
      721 gcatcatccg gagcagagcc cgtcaggttt ttcggtggaa aaagatagac cgagcggaaa
      781 gtgtcgtaat ctttttctga aagtccgctt gaatatcttg ctgcgacgct ggccgtgtat
      841 gtttgcccgt aaggcagggg agcaaaatcc caggaagtct gtgaagtcat gcccagcagg
      901 gccccatcca ggaacaccgc aaaatcgagg taccctgcag ccggagcagg cacaccgacc
      961 ctgacattat ctactgccca gcctgaagcg tactcgccgc cgtcgtcggc atggaaagcc
     1021 agccaaatct ctgaatagcc gttttgtccg ctgaatgccg aaaggtcgac gtccatcgaa
     1081 tgccaggagg tatcgggcgg caggttgtaa agagtttccc aagaaccgcc tccgtcggtg
     1141 gaatattcaa ccgatgccaa ttgtccgtaa aagccatcat aataactatc gaaactgagg
     1201 acaaagcctt cactttctcg caggtcaaga gccggtgtga ccaggtagtc gcagcatcca
     1261 tcgctctccg acccggcggc atcatcattg acataagcat aaaagctgtc atgcgccgga
     1321 attgtccagt tcggactgct gccattggag cccctcatcc agcctccagc ctggcttgag
     1381 gtcaggtttt gccatccgga gggtggaaaa acagcagatt caaaatcctc atccagcaat
     1441 gagaacagtg gctcgcacca attacccatc agcgagaggg aatcaacctg aaggcaggtg
     1501 ggcgggtatt tcttctcact taagacaact tcgaaagaaa catcatcttc aatatattca
     1561 tctgtaataa gatatggatc atatccgatc ttgtatatcc gaatgtcata atgccctttc
     1621 caaaccggct ccagtattgc attcccgtcc ggatcggttg tttccgtaat cgttgcataa
     1681 ggaaaatcat acccggtcat cgaaatttcc actccggaag gttcttcccc cgtactgagc
     1741 gaaatactaa agccggcggt gcaatccatc attcttccaa ccgtattgga aatcctgaaa
     1801 tcggagtaca atccactggt aaacaatgcc ctgaccccat aagcatacca gccttgctgc
     1861 aatgacccaa atgcgctgtc ggggtacgac aggtcggtca gcgtatccag aatggtttca
     1921 ttaccggtct ggggaggcag atcgggatca aaatccgaaa accggactac ctggtatgag
     1981 accacatctc tgactgcagg cctgttcaca ccggtcagct cgaattcata tcgctcatag
     2041 ccgggaacga gtaaagggat attcccgctt ttggtataag catatttatt ccaggaagat
     2101 gggattttgg ggatcgcttt cagtgataac tgcggaacgg gcattttcat atcattctcc
     2161 ggtcccgaga tccaggccct gatcataaaa tcctggagtg gcgacagcat ccatccatta
     2221 tcctgaaatt ggatataact gcgatattgg acgggattgt cggaatcgat gccgatgggt
     2281 gcggcaaaag ggacatttcc tgcctggtac atggcgaggt aaaaacttcc ttcggtaatc
     2341 agaacattca atccgtcgag gctgacccat cccgaattat tgacggtaac gccggtagaa
     2401 tcgagcatgg tgccgggttt tccgtcaacg ccatcatcat caaaaacggc tactccaaaa
     2461 tcggtcccca ggaaaggccc cggaaaactg ccatcaccaa cataaaactt tcctcccagg
     2521 accagggccg gataccctgt tggcataaat ttaacggccg accagctccc ggcctgtgca
     2581 taaacgaaaa aatcatcggc ttctccatca tccatgatca cttcataagg accgtttggc
     2641 aggctccagg cgacattgca ccaggtttca ccggcattga ccgaggcgct gacaaatccg
     2701 ggggcataat tcatgtccca gaggcctgta ttaaccgggg taatatttcc ctgagcggta
     2761 gcggtatcat atatcgtgac cggaatatag cccagtttac cgaacaccac atcatacctt
     2821 ccttcatcta caaaaagagt gtattcacca ttttcgccgg tcgttgtctg aaagggtccg
     2881 gcagttaccg aaacgccatt gaggggttca tcgttatcca tatccgtgac gacaccgctg
     2941 aaaataccgg gcagataaac atacatggta ttctccaata ccatctgatc attttcgata
     3001 tcattggtaa acagcaacaa atcgtcagaa taagcacccg gcccgtaatc ctttgaatca
     3061 taggtaattg ttacgacggt tgaagtacct tgcgggatgg taccggaggc cggagtcacc
     3121 tgggtgataa aatcggccgg aacactgtaa aggataacca ggtcatcatg aagatattcc
     3181 tggttaaatg ccacctgcaa tcctacctcc gggtcatggg attgaagccc gacggtgcag
     3241 gtgttcagga tcacaccttc atcaatatct tcatatataa caatgatctt cccattttta
     3301 ttgaggatga tttcggcatt gatatagaga tcatcctggt taaaatgctg gatattctta
     3361 aactggatgt agagcttgtt acccaccgtt tgatagaaca cctggctggt accggtaata
     3421 aaaacaaggt catcccacaa ccatgcgata aaatccctgt aaaccgagct gtttgttgga
     3481 ataccggtat ttcccagggt aatataattg ggggtaaaac cgacagcacc atttgaattt
     3541 atccaaaact gggttttatt ttctccataa tacggaaaat caaaaccgac cggaaaaggc
     3601 cctacgacgt tatcgtcgga gataccgaaa acaggatttc cggtggtgga gatatccagg
     3661 aatttgaaag aggggccttc cggctcatcg ttatcgatcc agacatatcc aaagtcatcc
     3721 gggccacctg cgccacggac cgaagagttg ccggccaggg ccagggcagc aaattcagga
     3781 aaggaaaacg acaatgtacc ttctccctgg ttggacaaag tggtaaaaac atccacctca
     3841 taaccaggcc agactgcacc ttccagttcg acaggattta tttgcagcaa tggatcgggc
     3901 cattcgatgc atgcaggtcc ggcaggtgat gtaacgcctt catggtaaac agcctgaacc
     3961 gtatagcaga agattccatg gttctcaaga atatcctgaa aggaggtctg tgttggggta
     4021 gcgataattt gcccgtttcg ccggatcagg aaaaattgaa aggcatcccc gctccattcc
     4081 cacgataaat cgacaattcc atcgacatta tctaataggc tcacttcaag gttttcagga
     4141 gggatcagct cattccccat taccgtcata gtaaccggaa ctgtaatatt acccacattc
     4201 gggaccgaag aaaacatgat agcggccgaa taggtctgtc ccgaaacaga acccgttgca
     4261 tttaaataga ccggaacgtt ggccaggccg ccaaacggct caacatcacc ctggtaactt
     4321 cccatggtca accattccga attgatctcc ctgctctgtg cagagtaatc aatgacggat
     4381 tcccagtgta atgaccccgt tccggtatta agaatattga tatatgttgt gatatattca
     4441 cccggattaa gggtttgctc aatcagtaaa ggattgatca ccatttccgg ttgtgtgagg
     4501 gtgaaattct ggtaggtagt atcagcagaa attacggtga cggtaatcca tgagaagttg
     4561 taccccggtt tgccgcagcc gatatcatgc tccccttcgg gtaaacggtc caatatatag
     4621 tacccgttgt tgtcggaggt tgtaaacggc ccgttctctt tgcctatggt agcgcctgat
     4681 attgcaacac cataataatt aaatacataa cctgaaataa agccatcagc acgcgatgag
     4741 gccagacctg cagacaggcc aggtccgatc ataatcaaaa ataaaaggga tattttaatg
     4801 gttatcctca tttcaagaag ccggattaat aatgcggcat gacttactga tatacttatt
     4861 tttcaggtta atgttacaat gataagacat taaataatta agattggttg catgttttcg
     4921 gataatgacg aaaaaaaagg ccggcgaggc cggcctttca tttatcagtt atc
//
LOCUS       DAOQXS010000181         4972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17207_length_4972_cov_3.845638, whole genome shotgun sequence.
ACCESSION   DAOQXS010000181 DAOQXS010000000
VERSION     DAOQXS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17207_length_4972_cov_3.845638"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKM34_06190"
     CDS             complement(<1..463)
                     /locus_tag="PKM34_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNQ83213.1"
                     /translation="MNSLVFDIRRFTIHDGPGIRTTVFFKGCPLHCQWCHNPESREME
                     IEKVNMKRVFDGRDFHCEETIGKWYSEKEVMHEIEKDMVFYRESGGGVTFSGGEPLMQ
                     ADALAQLLHLCKERELHTVVDTSGYAEPAAIQKVMPLTDLWLFDLKLMDDLK"
     gene            complement(693..1325)
                     /locus_tag="PKM34_06195"
     CDS             complement(693..1325)
                     /locus_tag="PKM34_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83214.1"
                     /translation="MRKKGLVNKMIVRILRLLPVLIVLLQAGCSQQNDPVRQSVLRQL
                     DRYPQSTLQDIYKSFFQDEFGPGHMLEDTAAARKYLAYELANMTSGGNYTAEPCGAGL
                     NFYRVPLDLVKDEKISFNEYLTAFLESGSSFRIPATGEWKAKWGMIVLEIESMNLEIN
                     NFESDKKDLSKMLDEGETTVHHSESYSLAYDPHYRIMSRVQCERLVGSVR"
     gene            complement(1300..4140)
                     /locus_tag="PKM34_06200"
     CDS             complement(1300..4140)
                     /locus_tag="PKM34_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNQ83215.1"
                     /translation="MKKPVKIITAVILILVLAIILLPVIYKGKIKGILLDEAEKNLDA
                     RLDIESVNISLIKSFPSFTIGLKGLTIVGKGAFETDTLLQVESVTITTGFMDLLNGSP
                     YSIRKILIDRADVRMKVLADGRANWDIAPASEEPVMEEQTSTGGSFRLLLKDLRINNS
                     RFIYDDIPNLTYAGLEDFNFSMNGDLGDERTVLGLKAGIGSALVRYDGVEYLKNAKIT
                     WDAGIDADLKNSIYTFADNKLYINDFPLIFNGSVGLPDNGYDLDLSFSTPSGGFKALL
                     SLVPAVYARDFESVKTDGTVSFNGFVKGVYSEDGYPAFKVDLSVGNAWFRYPGLPAEV
                     NKININGTVENPGGDLDNTVIDIRELSLNLADNPVMARLYLKNPMSDPFIDMEVNIDL
                     ALADIGKLYPLSPGEQVTGQIMADVAVKGNLSDFEKLNYTKIEASGKLNSSGIRYSTA
                     YLAQPLQIDRISLSVTPAFLDLADLKIRAGNSDFGLTGKVTNYLGYYLKNETLGGIFV
                     LESKRIDANELLTFAGTEEEPSSSDTAAVSAFIVPPGLDLTLKISAGSVAWLDYDIRN
                     LNGTVRVKDQKLLLDGLTMNGMGGTLRMDGSYATTDPVNPTIDFSLGLKDISIRETFK
                     QIGIVEKFAPVAEKIAGDISGNFKLSGLLDETMMPKMESLLGAGDLTTTAIQVNNVNT
                     LNQLSNSLKMDQLRELKINGARIIVEFINGAMNVKPFDFKALGIDMNLGGRTSLDQTI
                     GYELKMKIPRTMMGGEANKVVDDLLATAGRAGADLKPGDQINVDAMIDGTITDPKVKL
                     NLAGMGKNMVENLKEQVKEEVEKKVEEVKDMAKEEAAKILAEADKQAQAILDLASRQS
                     EELLRNAQKLADETKKTANTQADNIVKEAKGKGFIAEKAAEKSAEEVRKQGDKKAQGI
                     MDEARKQSGSVLAKAKQEADKVKADAQKRIGQ"
     gene            4296..>4972
                     /locus_tag="PKM34_06205"
     CDS             4296..>4972
                     /locus_tag="PKM34_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ83216.1"
                     /translation="MKEHIAHMVRDRATRFGSDEVFRFMDKKDESYKSYSWESFASDS
                     EKIACSLISMGFGHESRIGIFSDNRLEWTLADIGILAVRGVVVPFFGTASVKQVQYIV
                     DETEMELMFAGNQEQLDKAIWLLDHSKTLKTVVYFPGATACADKRCLAWEQFLQLGEG
                     PVYNHEFQLAFDNAQPYDLATILYTSGTTGEPKGVMLGHDNFMECFLIHDKRLDITRK
                     DVSLCFLP"
BASE COUNT         1234 a         1245 c         1078 g         1415 t
ORIGIN      
        1 gtttcaggtc atccatcagt ttcaggtcga acagccataa atcggtcagc ggcataactt
       61 tttgtatcgc agcaggttcg gcatacccgg aggtatccac gaccgtatgc agttctcttt
      121 ccttacaaag atgcaataat tgtgcaagcg cgtcggcctg catcaacggc tcgccacccg
      181 aaaaagtgac tcccccgccc gattcccggt aaaacaccat atccttctca atttcgtgca
      241 tcacttcttt ttcagaatac cacttcccga tggtctcttc acaatgaaaa tcccttccgt
      301 cgaatactct tttcatgttc actttctcta tctccatttc acggctttcc gggttatggc
      361 accactggca gtgtagcgga cagcccttga aaaaaacggt ggtccggatt ccggggccat
      421 catgaattgt aaaccgacga atatcgaaaa cgagggagtt caaaatttaa aattttatac
      481 ttaaattagc tgcatgtata tggtctcgaa aaacttacga cctccgaccg acttcacgtc
      541 atacgacaac aaaacaatcc ccggccgcgg cgagtgtaca tttggaagag atgataatgc
      601 ggcaatagca tgggtgaatt ttttgacaaa gataaaaagt tactctcgaa ttgcaagcgg
      661 ttctatgttt tgggctttag ccctgcactt cttcaacgaa cagatcctac caacctttcg
      721 cactgtactc tgctcatgat acgatagtgc ggatcatatg cgagcgagta agattcgcta
      781 tgatgaactg tagtttctcc ttcatccaac atcttcgaca agtctttctt atctgactca
      841 aaattattga tttccagatt cattgattct atttctaata caatcatccc ccactttgct
      901 ttccattcac cggttgccgg aatcctgaaa gaggaaccac tttccagaaa ggcagtcaga
      961 tattcattaa atgaaatttt ttcgtctttc accaggtcaa gcggaaccct gtaaaaattc
     1021 aacccggcgc cacagggttc agccgtgtaa ttgcccccgg aggtcatgtt ggccagctcg
     1081 taagccaggt actttcgtgc cgcggcagta tcttccagca tatgccccgg cccgaattca
     1141 tcctggaaaa aactcttgta aatatcctgg agggtggatt gaggataccg atccaactgt
     1201 cgtaaaaccg attgcctgac cgggtcattc tgctgactgc aaccagcctg gagtagaact
     1261 ataagtacag gtagcaggcg taaaatgcgt acaatcattt tattgaccaa tccttttttg
     1321 cgcatctgcc ttgactttat ctgcttcctg tttcgctttg gccagcacag atccggattg
     1381 tttccgtgct tcgtccataa ttccctgagc ctttttatcg ccctgtttcc gcacctcttc
     1441 tgctgacttt tcggccgcct tttccgcgat aaatcctttc cctttggctt ctttcacgat
     1501 attgtcggcc tgtgtattag cagttttctt tgtttcgtca gccagttttt gcgcatttcg
     1561 cagcagctct tccgattgcc tggaggcgag gtcgagaatt gcctgagcct gcttatccgc
     1621 ttccgccagg atcttcgcag cctcctcttt tgccatgtct ttcacctcct ccactttctt
     1681 ttcgacctcc tccttcacct gttctttcaa gttttcaacc atgttcttgc ccatccctgc
     1741 caggttgagt tttaccttcg ggtcggtaat cgtcccatcg atcatagcat cgacatttat
     1801 ctggtcgccg ggtttcaggt cagctccggc acgtccggca gtcgccagca agtcgtcgac
     1861 cactttattt gcctcgccgc ccatcattgt cctgggaatc ttcattttca attcgtagcc
     1921 aatggtctga tcgagcgacg tccggcctcc caggttcata tcgatgccca gtgctttgaa
     1981 gtcaaatggc ttgacattca tcgcgccgtt gatgaattcc acaatgatcc gggcaccatt
     2041 gatcttcagt tcccgaagct ggtccatctt caatgaattc gacaactggt taagtgtatt
     2101 cacattattg acctgtatgg cggttgtagt gagatcgccg gctcccagga gcgattccat
     2161 cttaggcatc atcgtttcat ccagcaaacc tgaaagcttg aagtttcccg aaatatcgcc
     2221 ggcaatcttt tcggcaaccg gggcaaactt ttcgacaatc ccgatttgtt taaacgtttc
     2281 gcggatactt atatctttca gtcccaggct gaaatcaatg gttggattga ccggatcggt
     2341 tgtggcatag cttccatcca tccggagtgt ccctcccatt ccattcatgg taaggccatc
     2401 aagcaaaagc ttttggtctt taaccctgac tgttccgttc aggttgcgaa tatcgtaatc
     2461 cagccaggca accgacccgg ctgaaatttt gagtgtcaga tcgagaccgg gcggaacgat
     2521 aaaagcagaa acggcagccg tatccgatga ggagggctcc tcctcagttc ctgcaaatgt
     2581 taataactca ttggcatcta tccgtttcga ttccaggacg aagattcctc ccagcgtttc
     2641 atttttaaga taataaccca ggtagttggt gaccttgccg gtcaacccga aatcgctgtt
     2701 cccggcacga attttaaggt ctgccagatc aaggaatgcc ggtgtgactg atagggagat
     2761 cctgtcgatc tgaagcggtt gggccaggta agcagtggaa tatctgatac cagaggaatt
     2821 cagtttaccc gatgcttcaa tctttgtgta attaagcttt tcaaaatcag acagattacc
     2881 tttcacagcc acatcagcca tgatctggcc ggtcacctgt tctccgggcg acaacgggta
     2941 gagtttgccg atatccgcca gtgccaggtc aatattgacc tccatatcga tgaaaggatc
     3001 cgacatggga tttttaagat aaagccttgc catgacagga ttatcggcaa ggttaaggct
     3061 cagttcgcgg atatcgataa cggtattgtc aagatcgccg cccgggtttt cgacagtccc
     3121 gttgatatta attttattga cctccgccgg aagaccggga tacctgaacc aggcgttacc
     3181 cacactgagg tcgactttga aagcaggata gccatcctct gaatatacac ctttgacaaa
     3241 accattgaaa ctgactgttc catcggtttt tacagattca aaatccctgg cataaacggc
     3301 tggtaccagc gaaagcagcg ctttaaaacc gccggacggt gtcgagaaag aaaggtccag
     3361 gtcatagcca ttgtcgggca gcccgaccga accgttgaag atcaacggaa aatcattgat
     3421 atacaactta ttatcggcaa acgtataaat actgttcttc aaatcggcat cgataccggc
     3481 atcccaggtg atcttcgcgt ttttcagata ctccactccg tcgtaacgga ctaaggccga
     3541 gccaattcca gccttcaaac ccaatacggt cctttcatct cccagatcgc cgttcatgga
     3601 aaaattaaaa tcctccaggc cggcataggt caggtttgga atatcatcat aaataaaccg
     3661 ggaattgttt attctcaaat ctttcagtaa aagcctgaag cttccgcctg tactggtttg
     3721 ctcctccatt accggctctt cggaagctgg ggcaatatcc cagttggcgc ggccatctgc
     3781 caggactttc atgcgcacat cagccctgtc gatcagaatt ttccggatac tgtaagggct
     3841 tccgttcagc aggtccataa aaccggttgt gatggtaacc gattcgacct gaagcagggt
     3901 atcggtttca aaggcccctt ttccgacgat ggtcagtcct ttcaatccta tggtaaatga
     3961 aggaaagctc tttatcaggg aaatattgac actttcaata tccaggcggg cgtcaaggtt
     4021 cttttcggcc tcatcgagta aaattccttt gattttccct ttatagataa cgggtagcag
     4081 aattatcgcc aataccagga tcagaatgac ggctgttata attttaaccg gtttcttcat
     4141 gtttactaag attatgtaag gatttccatt acagaaaaag caaatttaat cataaataag
     4201 acaagttttt ttaaccattt acatgttaaa tattgaatat ttaataaaaa atctcacatt
     4261 tgcgcctgtt attcaataat ttatttccag acaaaatgaa agagcacatt gcccatatgg
     4321 ttcgtgaccg tgccacccgg ttcggttccg atgaggtttt ccggttcatg gataagaaag
     4381 atgaatcgta taaaagttat tcatgggaat cgtttgccag tgattcggag aagatcgcct
     4441 gctcattgat ttccatgggt ttcgggcacg aatcccgcat cggtattttc agtgacaacc
     4501 gtctggagtg gacattggcc gatattggca tcctcgctgt caggggtgta gtggtaccgt
     4561 ttttcggcac tgcttcggtc aaacaggtac aatatattgt cgatgaaacg gagatggagt
     4621 tgatgtttgc aggtaatcag gaacaactgg ataaagctat ctggctgctc gatcattcga
     4681 aaacgcttaa aacggttgtt tattttcccg gcgcgacagc gtgtgcggat aaaagatgcc
     4741 ttgcctggga acaattctta cagctgggtg aaggcccggt gtataaccat gaatttcagc
     4801 ttgcttttga taacgcacag ccctatgacc tggccacgat tttatataca tcgggcacta
     4861 ccggcgagcc caagggtgtc atgctggggc atgataattt catggagtgc ttccttattc
     4921 acgataaacg attggatatt acccgcaagg acgtatcact ttgtttcctg cc
//
LOCUS       DAOQXS010000182         4966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17227_length_4966_cov_4.056404, whole genome shotgun sequence.
ACCESSION   DAOQXS010000182 DAOQXS010000000
VERSION     DAOQXS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17227_length_4966_cov_4.056404"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKM34_06210"
     CDS             <1..964
                     /locus_tag="PKM34_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNQ83217.1"
                     /translation="KILFIRFSSIGDIVLTTPVIRCVKQQVQGAEVHVAVKKAFLPVV
                     SANPYIDKIHVLGDDLKALTGLLRKERFDFIADLHCNIRSFYIRLSLHRPSAGFPKLN
                     FRKWLLTRFHINRMPGIHIVDRYFRAVEPLGVKNDGKGLDYFIPDDEEIGMASLPAEF
                     RKGYTAFVIGAKHATKKLPEHKIISICRKMNSPVILLGGPEDAPAGDRIAAACGPRVM
                     SMCGKATLNGSASLVKQADAVITHDTGLMHIAAAFRRRIVSVWGNTVPELGMYPYLPG
                     DEERSLIVEVKGLRCRPCSKLGYDRCPKGHFRCMEEINDSSFTS"
     gene            complement(1030..1674)
                     /locus_tag="PKM34_06215"
     CDS             complement(1030..1674)
                     /locus_tag="PKM34_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ83218.1"
                     /translation="MLNIHRFSFNPFQVNTFILWDETRECAIVDPGCYSKAEQDELRD
                     FIRENGLKPVRLINTHCHIDHISGVAFVSREYGLKPEAHFDGIPFIRHSEKTSFIYGF
                     DSLETTDPQLHLKEGDIIRFGNSELEVVEAPGHAAGSVCFISREGKFVITGDVLFHQS
                     IGRTDLPTGDYDLLIRNIREKLLTLPPDYTVYAGHGPETTIGYEMYSNPFLEKE"
     gene            1798..>4966
                     /locus_tag="PKM34_06220"
     CDS             1798..>4966
                     /locus_tag="PKM34_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83219.1"
                     /translation="MFWAAFAVFMALLLARPSSAQFYNGSQMSFGKNRIQYKEFLWTY
                     YKFEDFDIYFYLNGMELAVATAKYATEQIPLMEKKLQTSLDQKIQFLVYNNLTDLKQS
                     NIGLMQDERYNTGGITYIIDSKAFIYFSGEHRDFERQIRAAIANILINQAFYGTSVGS
                     QVKNSTLFTLPEWYRNGLVSYLSEDWNTEIDNKVKDGLLSGRYKRLNTLVGEDALYAG
                     HMLWKYIADKYGTQALTDIVSMTQISRSVDNGFLYVIGLSYKSLVAECLAHYTKIAEA
                     EEAGRSTPDESFLKKIRKRSVYSEFTFSPGKMFAAYAENDEGKYKVFLQNLRTGKKFS
                     VLRGGFRLEEKIDYSYPLITWHPDGNTLSIIVEKRGEVYLYSYNIEERKRNFIILYEF
                     EKITDFSYSPDGRYLVMSAVQKGQSDLYLYNIAATSLQQLNNDIYDDLNPVFAEGTGQ
                     ILFSSNRPDEKLAWMDYEDAPAAVGKTTDLFLMIRGDNKNILRRVTNTPLGSELRPEM
                     TDKGRFMFLSDENGINNRYFGQFDSAISFIDTTIHYRYFFETYPATNYSRSILSHEID
                     TVSGSLSQVVFQRGKYHLQEEELFPGTPDSVSLSQTAYRTVAVIEQEVKKEEEKAETE
                     LESYGEYTRRFRNVYRKDEQPAQPPPSDNSRIDIDNYVFEPTVTVTERGQDTVKQQPS
                     PDPNTKIEVKEGFVIPKRLNYRVEYTINQMGAQLDFTALNYFYQPFSGGSGLGISNLG
                     TNGNGFFQVGLNDLLENHRITGGFRVPLGISNVEYMFSYSDIEKRLDKEWLVVRQAIE
                     NQYTDGIYTDIIRFRSYQLFYMLKYPFSPVFAIKGTANVRYQKSTYLSTSEATLRAPD
                     SVEFWGGVKGELIYDDTKDIAPNLMTGTRFKIFGEYTQVLSEDSKGLFVVGLDVRHYL
                     RIHRSFIWANRLAASTSFGRNKLIYFMGGVDNWYRPKFSTEAPIDYSMNYTYQAIATN
                     MRGFKQNARNGNNFVVLNSELRFPVVRYFFNRPVKSDLLSNFQVVAFGDVGLAWAGED
                     PYSENNSFYIRYIQDGSLWIKIREQK"
BASE COUNT         1281 a         1194 c         1287 g         1204 t
ORIGIN      
        1 aaagatcctc ttcatccgct tcagctcgat cggcgatatt gtgctgacca cgcccgtgat
       61 ccgttgtgtt aaacaacaag ttcagggcgc cgaagtccat gtcgccgtaa agaaggcgtt
      121 cctcccggtc gtatcggcca atccgtatat cgataaaatc catgtgctgg gtgacgatct
      181 gaaggctttg accggtttac taagaaagga gcgctttgat ttcatcgccg acctgcattg
      241 caatatccgg tcgttttata ttcgtctctc actgcatcgt ccatcggccg ggtttcccaa
      301 actgaatttc cgtaaatggt tgctgacccg tttccatatc aaccgcatgc ccgggattca
      361 cattgtcgac cggtatttca gggccgtgga gccactgggc gttaaaaacg acgggaaagg
      421 gctggattat ttcattcccg atgacgagga aatcgggatg gcgtccctgc cggcagagtt
      481 ccggaaaggc tacacagcat ttgtcatcgg cgccaaacat gccaccaaga aactgcctga
      541 gcacaagatc atttccattt gccggaaaat gaacagcccg gtaatccttc tgggcgggcc
      601 ggaagatgcc cctgcgggcg acaggattgc agccgcctgc gggccacgcg tcatgtcgat
      661 gtgcggaaaa gcaaccctta acgggagcgc gtcactggta aaacaggccg atgccgtcat
      721 cacccacgat acaggcctga tgcatattgc cgccgctttc cgcaggcgga tcgtctcggt
      781 atggggaaat acggtgccgg aactggggat gtacccgtat ttgcccggtg atgaagagag
      841 atcattgatc gttgaggtga aaggccttcg ctgccggccc tgctccaaac tgggctacga
      901 ccgctgcccg aaagggcatt tccggtgtat ggaggagatt aatgattctt cgttcacgtc
      961 ataagttcac ttcgttcacg tgattagctc gctgcgctcg cgtcattggt caggaaagga
     1021 atcctaaact tactcctttt ccaggaaggg attactgtac atctcgtaac cgatggttgt
     1081 ctcggggccg tgccctgcgt aaacggtgta atcgggcggc agggtgagca gtttctcacg
     1141 gatgttccgg atcagtaaat cgtaatcgcc tgtcggcaga tcggtccgcc cgatgctctg
     1201 gtggaaaaga acgtcaccag tgatcacgaa tttgccttca cggctgataa aacacacact
     1261 gccggcggca tgcccggggg cctcaaccac ttccagttca ctgtttccga atcggataat
     1321 atcaccctct ttcaaatgta attgcggatc cgttgtttcc agagagtcga agccatagat
     1381 gaaactggtc ttctccgaat ggcggataaa cgggatcccg tcgaaatggg cctccggttt
     1441 cagcccgtat tcccggctta caaaggccac accggagatg tgatcgatat ggcagtgtgt
     1501 attgatcagc ctgaccggtt ttaatccatt ttccctgata aaatccctga gttcatcctg
     1561 ttccgctttg ctgtagcacc ccggatctac gatagcgcac tctctggttt catcccagag
     1621 gatgaatgta ttgacctgga aagggttgaa tgaaaagcga tgtatgttga gcatatcctt
     1681 aatacattaa gagcgggcaa agaaacaaca ttttcccgat tttttagttt attttagtgg
     1741 ctgatttcag gctgtacaga caggatggct aaaagcttta caacgaataa gttacgattg
     1801 ttttgggcag cgtttgcggt gttcatggcc ctgttgcttg ccaggccgtc ctctgcccag
     1861 ttttacaacg gcagccagat gagtttcggg aagaaccgga tccagtacaa ggagttcctg
     1921 tggacctatt acaagttcga ggatttcgac atctattttt acctgaacgg gatggagctt
     1981 gccgtggcaa cggcaaagta tgccacagag caaatcccgc tgatggagaa gaaattgcag
     2041 accagcctcg accagaagat tcagttcctg gtttacaata atctcaccga tctgaaacag
     2101 agcaatatag gccttatgca agacgagcgg tacaatacgg gtggtattac ctatattatc
     2161 gacagcaaag cctttatcta tttcagtggc gaacaccgcg attttgaacg ccagatcagg
     2221 gctgccattg ccaatatcct gatcaaccag gccttctatg gcaccagtgt cggttcgcag
     2281 gtgaaaaatt ctaccctgtt caccctgccg gaatggtacc ggaacgggct ggtatcctat
     2341 ctctcagagg actggaatac ggagatcgat aataaagtga aagacgggtt actgtcaggt
     2401 cggtataagc ggctgaatac gctggtcggt gaggatgcac tctatgcggg acatatgctc
     2461 tggaaatata tcgccgataa atacggtacg caggctttga ccgatatcgt cagcatgacc
     2521 cagatcagcc ggagtgtcga caacggtttc ctgtacgtca ttggtttatc ctacaaaagc
     2581 ctggtggccg aatgcctggc acattatacg aaaattgcgg aggcggaaga agccgggagg
     2641 tcgaccccgg atgagagctt cctgaagaag atcagaaaga gaagcgttta tagcgaattt
     2701 acattcagcc ccgggaaaat gtttgccgcg tatgccgaaa atgatgaagg aaaatataag
     2761 gtgttcctcc agaatctccg caccggaaag aagttttctg tgctgcgcgg cggattccgg
     2821 ctcgaggaaa agatcgatta ttcctatcct ttgatcacgt ggcatcccga tggcaatacg
     2881 ttaagtatca tcgtggagaa aaggggagag gtatatcttt attcctataa tattgaggaa
     2941 aggaaacgaa actttatcat actctatgaa tttgaaaaga ttaccgattt ttcttattca
     3001 cccgacggcc gttatctggt tatgtcggct gttcagaaag gacagtcgga cctgtatctt
     3061 tataatattg ccgcaacatc tctccagcag ctcaacaatg atatttacga cgacctgaat
     3121 cccgtttttg cggaggggac ggggcagatc cttttttctt ccaaccgacc ggacgagaag
     3181 ctggcctgga tggattacga agacgctccg gcagcggtgg gcaaaaccac cgatctcttc
     3241 ctgatgatcc ggggcgacaa caaaaacatc ctcagaaggg tcaccaacac ccccctggga
     3301 agtgagctgc gaccggaaat gacagacaag ggcaggttta tgttcctgag cgacgagaat
     3361 gggatcaaca accggtattt cggacagttc gacagcgcca tcagttttat cgatacaacc
     3421 attcattacc gttacttttt cgagacctac ccggcgacca attattcgag gagtatcctc
     3481 agccatgaaa tcgatacggt ttcaggatcc ttgtcccagg ttgtcttcca gagaggtaaa
     3541 tatcatttgc aggaagagga attgtttccg ggcacacccg attcggtatc gctgagtcag
     3601 acagcctacc ggaccgttgc cgtcattgag caggaggtta aaaaggagga agaaaaagcg
     3661 gaaaccgaat tggagagtta cggcgagtat acacgccggt tccggaacgt ttaccggaag
     3721 gatgaacagc cggcgcagcc ccctccttcc gacaacagcc ggatcgatat cgacaattat
     3781 gtatttgaac cgaccgtgac agtgacggag agagggcagg atactgtcaa acaacaacct
     3841 tcgcccgatc caaacacgaa gattgaagtg aaagagggct ttgttattcc caagcgactg
     3901 aattacaggg ttgaatatac gatcaaccag atgggagccc aactggactt taccgctttg
     3961 aattatttct accagccttt cagcggtggc agtggcctgg ggatatccaa cctggggaca
     4021 aacgggaacg gctttttcca ggtcggcctg aatgatcttc ttgaaaatca caggatcaca
     4081 ggcggtttca gggttccgtt aggcatctcc aacgtggaat acatgttcag ttattccgat
     4141 atcgaaaagc ggctcgacaa ggagtggctg gtcgtaaggc aggccattga aaaccaatac
     4201 acggatggaa tttataccga tatcatccgc ttcaggtcgt accagttgtt ttacatgctg
     4261 aaatatcctt tttccccggt atttgccata aaaggcacgg cgaatgtcag gtaccagaaa
     4321 tcgacttacc tgtcgaccag tgaagccact ttaagggctc ccgacagtgt tgaattctgg
     4381 ggaggcgtga aaggagagct gatttacgat gatacaaaag atatcgcacc gaacctgatg
     4441 accggcaccc ggtttaaaat tttcggcgaa tacacacagg ttctcagtga agacagtaaa
     4501 ggtctgtttg tggtaggtct tgatgtgcgc cattacctgc gaatccaccg gtcgttcatc
     4561 tgggcaaacc ggctggcggc cagtacatct ttcggcagga acaagctgat ttactttatg
     4621 ggaggcgtcg ataactggta caggcccaaa ttttccacgg aagccccgat cgactattcc
     4681 atgaattata cctaccaggc gatagctacg aatatgcgcg gattcaaaca gaatgcgcgg
     4741 aatggaaata atttcgtggt actgaacagc gaactgagat tcccggtggt caggtatttc
     4801 tttaacaggc cggtaaaatc agacctgctc agcaatttcc aggtcgtggc tttcggcgat
     4861 gtcggcctgg cctgggcagg ggaagacccc tattccgaaa acaattcatt ttatatccgg
     4921 tatattcagg atggttcgtt atggatcaag atcagggaac agaagg
//
LOCUS       DAOQXS010000183         4946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17324_length_4946_cov_4.677162, whole genome shotgun sequence.
ACCESSION   DAOQXS010000183 DAOQXS010000000
VERSION     DAOQXS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17324_length_4946_cov_4.677162"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKM34_06225"
     CDS             complement(<1..471)
                     /locus_tag="PKM34_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83220.1"
                     /translation="MDNSKKGKCPVMHGANTDTNSSVMSWWPNALNLDILHQHDTKTN
                     PLGPGFCYREEFKKLDLEAVKADLKKLMTDSQDWWPADWGHYGGLMIRMAWHSAGTYR
                     VTDGRGGSNTGNQRFAPLNSWPDNGNLDKARRLLWPIKKKYGNKLSWADLFILAG"
     gene            complement(652..1425)
                     /locus_tag="PKM34_06230"
     CDS             complement(652..1425)
                     /locus_tag="PKM34_06230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ83221.1"
                     /translation="MSDENKSIHEFDFNLICEYFSLLERQGPGSPEQTLNALSFVGPL
                     SEDAHIADIGCGTGGQTMVLARNVPAKITGIDLFLRFIELFNANALNLGLGERVKGIV
                     GDMNILPFQEEELDLIWSEGAIYNIGFERGITQWRKFLKPGGYLAVTEASWFTDNRPD
                     EIHDFWMNAYPEIDTVPRKVAQIQQAGYIPIATFILPETCWTDHFYVPQMKAREIFLQ
                     KYRGNPMAEGLAANEKHEAELYAKYKDYYGYVFYIGKKI"
     gene            1756..3162
                     /locus_tag="PKM34_06235"
     CDS             1756..3162
                     /locus_tag="PKM34_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ83222.1"
                     /translation="MRRIFFIILVLAFLPGISPVHSQPYTPDWASVDSRPVPGWFSDA
                     KFGIFIHWGVYSVPAWAPASADIAIYAKYAEWYWWRINEISPAGKLFREYHDRMYGPG
                     FYYQDFAGDFKAEHFDPGQWADIFKRSGARYVVLTSKHHEGFALWPSAQSPNWNSVDI
                     GPHRDLCGDLTQAVKAAGLHMGFYYSLYEWYHPLYQSNVNQYVDQHMMPQLKDLVTRY
                     EPELIFTDGEWDHPSETWKSTQFLAWLYNESKVKEKVVVNDRWGKETRSVHGGYFTTE
                     YDLVHDNKGIGETSRPWEECRGIGTSFGYNRIETTEHYMTSAALIRLLVEKVSAGGNL
                     LLDVGPTADGLIPVIMEQRLLDMGEWLKVNGEAIYGTQPWKNRPAVAKEETAGKEQNV
                     YYTTKGKDLYVICTRWPEKAVIVPGVAKPASVTLLGSSLPVKSRWASGTLTLSPPSIH
                     PGNIPCHHAWVFKIAGGL"
     gene            3333..4082
                     /locus_tag="PKM34_06240"
     CDS             3333..4082
                     /locus_tag="PKM34_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNQ83223.1"
                     /translation="MKKLPNAPLQEVIFEGKWELSLDQQTSAFIDPEFQFALGKFQHV
                     ISSRFPYHKSKIPGDIPLQFLGHQPIHQFWTGEGVWPVIQLGPGVITVNDTEKNYEWD
                     DVFFPLVKETLFNVKNAYVNDLSFISYTLRYIDVVKIKDYEFSNWFDFVKDNINFQFS
                     NGYPLPGTLHNFKIEQSFRTEDAGLIEIGFSNGQDNQKEDIFIWQTAVTGRKPLFIEG
                     LMEWLVLAHSHCSRIFKETCKEDFYASFVNR"
     gene            4111..4548
                     /locus_tag="PKM34_06245"
     CDS             4111..4548
                     /locus_tag="PKM34_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83224.1"
                     /translation="MQLAEDKSSLIWHFRIRNLIAPNSTVFPDLLSFPPVIISFIQKL
                     DSYINLNDNWDSYGAVAPSVISIRNARRFLINNANVSLPYYFISPGVNGEVMIELSKG
                     RKAAEIYFNSDKSTELLLFENNNCLLESNLKDSIKELIKFFND"
BASE COUNT         1314 a         1161 c         1139 g         1332 t
ORIGIN      
        1 tcccgccagg ataaacaggt cggcccagga gagcttgttc ccgtatttct tcttgatggg
       61 ccacaggagg cgacgggcct tgtccaggtt gccgttgtcg ggccagctgt tgagcggggc
      121 aaaccgctgg ttcccggtgt tgctgccacc acggccgtcg gtaacccggt aggtgccggc
      181 actgtgccag gccatgcgga tcatcaaccc gccatagtgc ccccagtcgg ccggccacca
      241 atcctgactg tcggtcatca gcttcttcag atcggctttc accgcttcca ggtcaagctt
      301 cttaaattcc tcacggtaac aaaaacccgg gcccaaggga ttggtcttcg tatcgtgctg
      361 gtgcaaaatg tccaggttaa gggcattggg ccaccaactc atcacggaac tgttcgtatc
      421 cgtgttcgcc ccgtgcatca cagggcattt tcctttcttt gaattgtcca tggttttgat
      481 tttttggttt aaatataacg attttaaata aatcttcatc aaaattagaa taattctaat
      541 tgagaaatgc aatagctatc ctttctggcc cgattttttc ggatcaggaa aatctgtttc
      601 cgtgaaattg gatcagtatg ccatttatac tgaaccagga gggaaaatca tttatatttt
      661 cttcccgata taaaatacat acccgtaata gtctttgtat ttggcataaa gttccgcttc
      721 atgcttttca ttggccgcca gaccttccgc catgggattc cccctgtatt tctgaagaaa
      781 gatctcccgt gccttcatct gaggcacgta gaaatggtcg gtccagcagg tttcgggcag
      841 aatgaaggtg gcaatgggga tatagccggc ctgttgaatt tgggctacct tgcggggaac
      901 ggtgtcgatt tccggatagg cattcatcca gaaatcatgg atttcatcag ggcgattatc
      961 agtgaaccac gaggcctccg tcacggccag ataacccccg ggcttcagga atttccgcca
     1021 ctgggtgatg ccccgttcaa aaccgatgtt gtaaatggcc ccttccgacc atatcagatc
     1081 cagctcctct tcctgaaaag ggaggatatt catatcgccc acgatccctt tcacacgttc
     1141 ccccagtcct aaattcaggg cgttggcgtt aaacagctca atgaaccgca ggaaaaggtc
     1201 tattcctgtg attttcgccg gaacattccg ggccagtacc atggtttgac ctcccgtacc
     1261 acatccgatg tcggcgatgt gtgcatcttc ggaaagagga ccgacaaagc tcagggcatt
     1321 taacgtttgc tcaggacttc cggggccctg ccgttccaat aaagaaaaat attcacagat
     1381 aaggttgaaa tcgaattcgt ggatggattt attctcgtca ctcatgatga tgaagtatta
     1441 agtagcctga aaaagctgaa aaccgcttag cacaggttac gtgatgaaac aattgtattt
     1501 tgatgatgaa aataagaaac acttccggca ggagaatgcc ggaggtttgc atttgtgctg
     1561 atccgggtta cccgggtcag ttacctcacc gaatccgtaa taaagctaaa catccaaagg
     1621 tattacactg acaaatatcg gaaaattacc gtaacaagtc atcgccggag cgaatcattt
     1681 ttcaggatga catttcgggt aaaattgctt acattagcct tcctgaacca gagcaaaacc
     1741 ataataaagc gccttatgcg aaggattttc tttatcattt tggtcctggc ttttttgccg
     1801 ggcatttcac ctgtacacag ccagccctac acgcccgact gggcatcggt tgacagccgg
     1861 ccggttcccg gatggttttc tgatgccaag tttgggattt tcatccactg gggggtctat
     1921 tcagtgcctg catgggcgcc cgccagcgcc gatatcgcaa tctatgccaa atatgccgag
     1981 tggtactggt ggcggatcaa tgaaatcagc ccggcaggga agctcttccg ggagtaccat
     2041 gaccggatgt atggaccggg attctactac caggatttcg caggagattt caaagccgaa
     2101 catttcgatc ccgggcagtg ggccgatatt tttaagcgtt ccggggcccg atatgtggta
     2161 ctcacctcca agcatcacga aggtttcgcc ctttggccca gtgcccagag tcccaactgg
     2221 aacagcgtcg acatcggtcc ccaccgtgac ctgtgcggcg acctgacaca ggccgtcaaa
     2281 gccgccggtt tgcacatggg cttttactat tcactctacg aatggtatca ccccctctac
     2341 caaagcaatg tgaaccaata tgtcgaccag cacatgatgc cccagttgaa ggatctggtc
     2401 acccgctatg agccggaact gatctttacc gatggcgagt gggatcaccc ctcggaaaca
     2461 tggaaaagca cgcaatttct ggcctggctc tacaacgaat caaaggtgaa ggagaaggtg
     2521 gtggtcaacg accgctgggg caaggagacc cgctcggtcc acggtggcta ttttaccacg
     2581 gaatatgatc ttgtgcatga caacaagggg atcggagaaa ccagccgtcc ctgggaggag
     2641 tgccggggca tcggcacctc tttcggctat aaccgcatcg aaaccacaga acactacatg
     2701 acttcggccg ccctgatccg gttgctggtg gaaaaggtgt ctgccggggg caatctgctg
     2761 ctcgatgtgg gtcccaccgc cgatggcctt atcccggtga tcatggaaca gcggctcctc
     2821 gacatgggag aatggctgaa agtgaacggc gaggccattt acggcaccca gccctggaaa
     2881 aaccggcctg ccgtagcgaa ggaagagact gcagggaagg agcaaaacgt ctattacacc
     2941 accaagggga aagatctcta cgtgatctgt acccgctggc ccgaaaaggc ggtgatcgtt
     3001 cccggtgtgg caaaaccggc atcggtgacc cttttgggca gcagcctgcc tgtaaaatca
     3061 cgctgggcaa gcggtacact caccctttcc ccgccttcga tccaccccgg caatatcccc
     3121 tgccaccatg cctgggtatt caaaatcgcg ggaggtttat aaccatgtgg ggcaggcagg
     3181 tcaaagagcg gctttttaaa gtcaggaagt tgataaatga gggggagtgt tttcttgatt
     3241 gatacaagct cacaattcgc tttctcgaac aaattattca gaaccatatg aatttcttgc
     3301 acatgaaata actgtatatt tgcaatatgt acatgaagaa attaccaaac gccccattac
     3361 aggaagtgat atttgagggg aagtgggaac tttctcttga tcagcaaacc agtgccttta
     3421 ttgatcctga gttccagttt gcattaggga aatttcaaca tgttatatct tcaagatttc
     3481 cataccataa atctaagata ccaggtgata ttccacttca gtttttaggc caccaaccca
     3541 ttcatcagtt ttggacaggt gaaggagttt ggccagtcat tcaattaggc ccgggagtaa
     3601 tcacagtgaa tgatactgaa aagaattatg aatgggatga cgtttttttt ccattggtga
     3661 aagaaacttt atttaatgtt aaaaatgcct atgtcaatga tttaagcttc atttcttata
     3721 ctttacggta tattgatgta gttaaaatta aagactatga atttagtaac tggtttgact
     3781 ttgttaaaga caatataaat tttcaattta gtaatggtta tccgttacct ggaaccttgc
     3841 ataattttaa gattgaacaa tcctttagaa cggaggatgc cggattaatt gaaatcggct
     3901 tttcaaatgg ccaggacaat caaaaagagg acatttttat ttggcagaca gctgtaaccg
     3961 gaagaaaacc attgtttatt gaaggattaa tggagtggct tgttttagca cattctcatt
     4021 gctccagaat ttttaaagaa acctgtaagg aggattttta tgcaagcttt gttaaccgat
     4081 gaattaaatc aatattcgac aaatactgtt atgcagctag cagaagataa atcttctctg
     4141 atctggcatt ttagaatcag gaacttaatt gctcccaaca gtactgtgtt tcctgatctt
     4201 ctatcatttc ccccggtaat aatcagtttc attcaaaaac tggacagcta tattaacctt
     4261 aatgataact gggattcgta tggcgcagtt gccccttcag taatttcaat caggaatgcc
     4321 cgaagatttc tcattaataa tgcaaacgtt tcacttcctt attacttcat ctcccctggg
     4381 gtaaatgggg aagttatgat tgagctaagt aaagggagga aggccgctga aatttatttt
     4441 aattcggaca aatctacaga attattgctt tttgagaata acaactgttt acttgagagc
     4501 aatttaaaag actcaatcaa agaactaatt aaattcttca atgattgata tcccctccat
     4561 cgtgactatc ggagaaaatg atcgcttgtt acgaagggtt caataccttc atcccgattt
     4621 cattaagcca gacgggacgc cttcctctgc aagttttagt ttaaaaaccg atgaaaaagg
     4681 tctttctgtc gacttagagc gtctgacttc gtatgaaaag tcaattctgg acaaaaccag
     4741 atttcgtttg ttttatcttt ctgcttcctt tacggtccag ctaggactta taaatcaaca
     4801 tgacccacag cccgacaatt atgcccatac attagtcatg ggcaacattt ctagatctgt
     4861 gagccgtcag cttgcggcag cagcaaaacg catttatttt ccgtaattat ccattgagaa
     4921 gcattcgaag gtttctcatc gcttcc
//
LOCUS       DAOQXS010000184         4940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17345_length_4940_cov_3.555169, whole genome shotgun sequence.
ACCESSION   DAOQXS010000184 DAOQXS010000000
VERSION     DAOQXS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17345_length_4940_cov_3.555169"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKM34_06250"
     CDS             complement(<1..534)
                     /locus_tag="PKM34_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83225.1"
                     /translation="MKKNYFIWIGLIMTVLFLSGCLTCEKKEYTFQFTGKDSGRLTIK
                     YINILSTMDDTIDVSEEDFASLMTDYIEGSEIENEFPDATVVDKRLFEENGVLCGEIV
                     LEFSNLTACHLYQHKGKGPIMYCLGCYSIDSEYYGSSNGEYGNDIMPVVFWEDGLKTL
                     TLTTDVTTPDETTVSLLD"
     gene            638..895
                     /locus_tag="PKM34_06255"
     CDS             638..895
                     /locus_tag="PKM34_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83226.1"
                     /translation="MKTSVEISYYPLDQEYVPPIKDFIGRMNRYEGLIVRTNGMSTQI
                     FGDYDCVMNALKDEIKISFQNPHSVFVMKVINTDLNTKEKE"
     gene            895..1512
                     /gene="pnuC"
                     /locus_tag="PKM34_06260"
     CDS             895..1512
                     /gene="pnuC"
                     /locus_tag="PKM34_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNQ83227.1"
                     /translation="MLISVFQTFYDNLLASSWLEAVAVAAGLMSVWYARREDILVYPT
                     GIVNVLIYVYLCYEVKLYADMSINIFYFGMSIYGWIKWTRKTSDNQTRPITVMDRKEW
                     ILSAGGLVLFFFALWYILQKYTDSNVPVWDALTTAIFIIGMWLMALKKVENWIFWIAG
                     DLISVPLYASKSLVLSSFQFAVFLALAIAGYIEWRKKFKLKTTSI"
     gene            1534..2049
                     /locus_tag="PKM34_06265"
     CDS             1534..2049
                     /locus_tag="PKM34_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83228.1"
                     /translation="MYRISITGPESTGKSALAKELAAHFGTVWVPEYAREYLEKLGRR
                     YEFEDIAIIAIGQLEQENRMAAKADQIVFCDTDMLVCKIWSEFRYGHCDAWLQKIVRS
                     HVYDLYLLCDIDLPWEEDPLRENPKERQELFDIYLRELQKMNANFGIVTGTGDQRLRN
                     AISAVVGRLSW"
     gene            2233..3378
                     /locus_tag="PKM34_06270"
     CDS             2233..3378
                     /locus_tag="PKM34_06270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83229.1"
                     /translation="MARWYPNRYDPMPGLFIERHARSVSEHYDMAVLYIHQDEELKNT
                     AFEIEKFRDKELFQVKLYYRPVQLRIPAIGRFINFLLYIFYSFKGLLLLEKEWGQPDL
                     IHVNVLTRIGAIALIHKWLTRVPYVITEHWTRYLPHMDNFRGRIRKAVTRLVVKEASA
                     VLPVTDNLRMAMESHGLTNKNYRIIPNVVDMNMFTVMDWTMTGKKHFIHVSCFEDRQK
                     NISGMLRVLKRLSETRDDWECVMIGEGIHLERLIDYAKELEIEGKFVRFTGLKENGEL
                     AELMAKTGFQVMFSRFENLPVVILESYACGVPVLSTDVGGIKEHLTDDLGILIRSEDE
                     EELFLKLNEMLDRYGSFDKNKIRDYAERHFSREVIGRQLAEIYREAI"
     gene            3391..4677
                     /locus_tag="PKM34_06275"
     CDS             3391..4677
                     /locus_tag="PKM34_06275"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83230.1"
                     /translation="MFGKIINTFGTRALAAVINLGIAIALSQYLGPAGKGVQSLIITT
                     ITFVLVFANLVGGATLVYLVPRREPSLLLLPSIVWTLVVSALSFAILQVFPLVDKTYS
                     AHICILSAINSFTSITTSILIGREKIRAANAVGLVQPAVLILSLFLFFGNSPSPGVDH
                     YIWSLYFSFIFSAIAGMIYYQRYCGKIRFFGMAEYLKVSLEMARYGVLNQVAHVTQML
                     SFRLSFYVLDRYHGEAAVGIYSNGISIAESIWLIAKSISLVQYARISNLNNREESAKL
                     TVSLMKFSLAASLVLILPVLLLPESFYLWVFGEGFGEMRYVIQALAGGVLVYNFSILA
                     GHYFSGTGRYQVNAITSTLGLIASIILYYSMIPSLGITGAGFATSLSYLFTSLILMYL
                     FGREYKTWYREIIPVKGDLIRLRDGLKEMMNKKPGS"
     gene            complement(4674..>4940)
                     /locus_tag="PKM34_06280"
     CDS             complement(4674..>4940)
                     /locus_tag="PKM34_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNQ83231.1"
                     /translation="EIRRLKLDLFGGCYNQRQIRGGTKWSMHSWGIAVDFDPARNKLE
                     WGRDKAAFAGQEYDKWWELWETEGWVSLGRQRNFDWMHVQAAKL"
BASE COUNT         1264 a         1083 c         1226 g         1367 t
ORIGIN      
        1 atcgagcagg ctgacggtcg tttcatctgg tgtggtcaca tcggtcgtta gcgtcagcgt
       61 tttcaggcca tcttcccaga ataccaccgg catgatatcg tttccatact ctccatttga
      121 ggagccatag tattcggaat caatggaata acatccgaga caatacatga tagggccttt
      181 gcctttatgc tggtacaggt ggcatgccgt taaatttgaa aactcaagca cgatctcgcc
      241 acaaagcacg ccattttctt caaataacct tttatcgact accgtagcat cagggaactc
      301 attttcaatc tccgagccct caatgtagtc ggtcatcagg ctggcgaaat cctcttccga
      361 tacatcgatc gtatcgtcca tcgtcgacag gatgttaata tattttattg tcagtctgcc
      421 agaatctttg ccggtaaact ggaaagtgta ttcttttttc tcgcaggtaa ggcagccgct
      481 gagaaataaa acggtcatta tcaggccgat ccaaataaaa tagttctttt tcatatgatt
      541 cttggtttat tgcgcggaat ttcttcaaac aaatttaagg atatccttca ataataaaag
      601 tgtttcatta tttttaattt cgtgaaaaat ataagagatg aaaacatccg ttgaaatcag
      661 ttattatccc cttgaccagg aatatgtgcc gcccataaag gattttatcg gcaggatgaa
      721 ccggtatgaa gggcttatcg tcagaacaaa tggaatgagt acgcagatat tcggcgatta
      781 cgactgtgta atgaatgctt tgaaggatga aataaaaatt tcttttcaga atccacattc
      841 cgtttttgtg atgaaagtca ttaataccga cctcaatacg aaagaaaaag agtgatgctg
      901 atctccgttt tccagacttt ttatgacaac cttttggctt cgtcatggct ggaggctgtg
      961 gcggtagcgg caggtctcat gagcgtctgg tatgcgcgcc gggaagatat cctggtttac
     1021 cctaccggaa ttgtgaatgt gcttatctat gtttacctgt gttatgaggt gaagttgtat
     1081 gccgacatgt cgatcaacat tttttatttt gggatgagca tttacggctg gataaagtgg
     1141 acgaggaaaa catcggacaa ccaaaccagg ccaatcacgg tcatggatcg aaaagagtgg
     1201 atcttgtcgg cgggcggttt ggtccttttt ttcttcgcat tatggtacat tttacagaag
     1261 tacaccgaca gcaacgtacc ggtttgggat gccctgacta cggctatatt catcatcggc
     1321 atgtggctga tggcattgaa gaaggtggag aactggatct tctggatagc cggagacctg
     1381 atatccgttc ccttatatgc cagtaaatcg ctggtattga gcagctttca atttgccgta
     1441 ttcctggcgc tggccattgc cggatatatt gaatggagga agaaatttaa actcaaaact
     1501 acttcaattt aacactaagt tttagtcctg attttgtacc gtatttcgat caccggcccg
     1561 gaatccaccg ggaagtcggc cctggcgaag gagcttgccg cacatttcgg tacggtctgg
     1621 gttcccgaat atgcccgcga atatcttgag aagcttggca ggaggtatga gtttgaggat
     1681 attgcaatta ttgccatagg gcagcttgaa caagaaaata gaatggcggc caaagccgat
     1741 caaattgtat tttgtgatac cgatatgctg gtttgcaaaa tctggagtga attcaggtac
     1801 ggacattgcg acgcctggct tcagaagatc gtaagaagcc atgtctacga cctgtacctt
     1861 ctgtgtgaca tcgatcttcc ctgggaagaa gatcctttgc gggaaaatcc aaaggaaagg
     1921 caggagttat ttgatatcta tctccgtgag cttcagaaaa tgaatgctaa ctttggcatc
     1981 gtgaccggaa ccggggatca acgtcttcgg aatgcaattt cagcggttgt cggacgtttg
     2041 tcctggtagg cagccggcag tcggcagtcg gcagtaagca gtcggcagtc ggcagtcata
     2101 agttcattag atcagaatat caaaatatca caagttcaaa atatcacaag ctcaaaatat
     2161 cataatatca aaatatcaaa aatcaaccca agaaacggca tgtcttctcc ggataaaaac
     2221 aaggttcttt ttatggcccg ctggtatcca aaccgttacg atccgatgcc cggtttattc
     2281 attgagcggc atgcccggtc ggtgtcggag cattacgaca tggctgtgct atatattcat
     2341 caggatgaag aattaaaaaa cactgcattt gagattgaga aattcaggga taaagagctt
     2401 ttccaggtaa agctttatta ccggccggta cagttaagga ttcctgcaat cgggcggttc
     2461 atcaatttcc tgctttatat tttttactcc ttcaaagggt tattactttt agaaaaggaa
     2521 tggggccagc ccgatctgat ccatgttaac gtattgaccc gcattggagc catagcactc
     2581 attcacaaat ggttaaccag ggttccatat gtgatcaccg agcactggac ccggtattta
     2641 ccgcatatgg acaatttcag gggaaggatc aggaaagccg tcacacggct cgtggtgaaa
     2701 gaagcttctg ctgttctccc ggtaaccgac aatttgcgga tggctatgga gagccacggg
     2761 ctgaccaata aaaactaccg cataataccc aatgtggtgg atatgaatat gttcacggta
     2821 atggattgga caatgaccgg caaaaagcac tttatccatg tttcctgctt tgaagaccgc
     2881 cagaagaata tatcggggat gctgcgcgtg ttgaagagat tgtcggaaac aagggatgat
     2941 tgggaatgtg tgatgatcgg cgaagggatc catctggaaa ggcttattga ttacgcaaaa
     3001 gagcttgaga ttgaaggaaa gttcgtccgg tttaccggct tgaaagagaa cggtgaactg
     3061 gcggaattaa tggcaaaaac aggattccag gtgatgttca gccggtttga gaacctgccg
     3121 gtggttatcc tggaaagcta tgcctgtggc gtgccggttt tgtcgacgga tgtgggcgga
     3181 ataaaagaac atttgactga cgacctggga attttgatca ggagtgaaga cgaagaagag
     3241 cttttcctga agctgaatga gatgcttgac cggtacggat ctttcgataa aaacaagatc
     3301 agggactatg ccgagcggca ttttagccgg gaagttatcg ggcggcaact ggcagagata
     3361 tatcgggagg cgatttaaca accaacgacc ttgtttggta aaatcataaa tacattcgga
     3421 acgcgggcgc ttgcggctgt gatcaacctg gggattgcga ttgcattgtc gcagtatctc
     3481 gggccggcag gcaagggggt acagagcctg atcataacta cgatcacttt tgtgctggta
     3541 tttgccaacc tggtcggcgg ggcgacgctg gtataccttg ttccaaggcg cgaaccctcg
     3601 ctcctgctac tgccttccat cgtatggaca ttggtcgtaa gcgccctgtc gtttgcgata
     3661 ttgcaggttt tcccgctggt tgacaaaacc tactcggctc atatctgtat actgtctgcg
     3721 ataaattcct tcacttccat cacaacctcc attctgatcg gcagggaaaa gatcagggca
     3781 gccaatgcag tcggtctggt tcagccggcc gtactgatcc tttcattgtt tctgtttttc
     3841 ggaaattctc cttccccggg agttgatcat tacatttggt cgctctattt ttcctttatt
     3901 ttttcggcaa ttgcagggat gatttattac caaaggtatt gcggaaagat caggtttttt
     3961 ggtatggcag aataccttaa agtcagcctg gagatggccc gctacggagt tctgaaccag
     4021 gtggcccatg tgacacagat gctcagtttc aggttgagct tttatgtgct cgaccggtat
     4081 catggcgagg cggctgtcgg gatttattct aacgggatct ccatagccga atccatctgg
     4141 ctgattgcca aaagcatatc gcttgtacag tatgcccgta tttccaattt gaataacagg
     4201 gaagaatcgg caaagctgac ggtcagtctg atgaaattca gcctggcagc cagcctggta
     4261 ttgatcctcc ccgtattatt gttacctgaa tcattttacc tgtgggtatt cggtgaggga
     4321 ttcggggaga tgcgttacgt tatccaggcc ctggcaggcg gtgtgctcgt ctataatttt
     4381 tccatcctgg ccgggcacta tttttcaggg acaggccggt accaggtcaa tgccattaca
     4441 tcgacactcg ggttgattgc ttcgataatc ctttattatt cgatgatccc atcgctgggt
     4501 ataacaggag ccggctttgc cacctccctg tcatatctct ttaccagcct gatcctgatg
     4561 tatttgttcg gtcgcgaata taaaacatgg tacagggaga tcattcctgt gaaaggtgat
     4621 cttatccgtt tgcgtgacgg gttgaaagag atgatgaaca agaaacccgg aagctagagt
     4681 ttggctgcct gcacatgcat ccagtcgaag ttgcgctgcc ggcccaggct cacccaccct
     4741 tccgtttccc agagttccca ccatttgtcg tattcctgcc cggcgaaggc agctttatct
     4801 ctcccccatt cgagcttgtt ccgggccgga tcgaagtcga ccgcaatccc ccagctgtgc
     4861 atcgaccatt ttgttccgcc cctgatctgg cgctggttat agcatccgcc gaacaggtcg
     4921 agtttgagcc gccggatttc
//
LOCUS       DAOQXS010000185         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17370_length_4936_cov_3.409342, whole genome shotgun sequence.
ACCESSION   DAOQXS010000185 DAOQXS010000000
VERSION     DAOQXS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17370_length_4936_cov_3.409342"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..601
                     /locus_tag="PKM34_06285"
     CDS             152..601
                     /locus_tag="PKM34_06285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNQ83232.1"
                     /translation="MFSDEYFMREALKEARKAFDLDEVPVGAVIVCNDRIIGRGHNLT
                     ERLTDVTAHAEMQAFTAAAGHLGAKYLDECTLYVTLEPCVMCAGAAFWTQIGRIVYGA
                     PDDKRGYTKTGAGLLYPRTTVTPGILAQDCADLLIEFFKSKRNDATE"
     gene            588..1346
                     /gene="rhaD"
                     /locus_tag="PKM34_06290"
     CDS             588..1346
                     /gene="rhaD"
                     /locus_tag="PKM34_06290"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF002963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HNQ83233.1"
                     /translation="MQLNKTNQGAQGVAAGIAEVASLLWQKGWAEASGGNISVNVTEF
                     YPNVRIDFRTYPMIPLPVKYPAIAHNYIFITSKGSRMRTLAKDPGSGICLVKISKSGE
                     AYQVLFEDPENPLVPSSELPSHFAIHNLLVKQKREEKAVVHAHVHEMVAMSHISGFNN
                     EARLNELLMGMHTETAYFIPEGMGYVPLLEPGSQELAKATLKSLKDHKVILWEKHGCL
                     ATGKDVDEAFDRIDLVAKAAKIYLLASHNRHIPS"
     gene            complement(1291..2217)
                     /locus_tag="PKM34_06295"
     CDS             complement(1291..2217)
                     /locus_tag="PKM34_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ83234.1"
                     /translation="MNKTKFIIILISGVLILFTSCNQQPPQQEKAQNEFTFAFLTDIH
                     VQREKDAISGFRKAITKVNELKPDFVITGGDLVMDALGQTYERADSLYHIYTTMMTEF
                     NMPVFNTMGNHEIYGWYVLKDGDTNHPEYGKKMFENRIGPRFQRIDRFGWIIFILDSV
                     VKDSTGDGYEGGVDAVQIAWLREQLASIGAETPIIISLHIPLLTTEAQILRGATAAND
                     RNEVVVNSKEVIDLFNGHNLKLVLQGHLHYYESLHVFGTNYVTAGAVSAAWWEGPFLG
                     TEEGFLLLKAKGDEISWEYVDYGWQAGKSSPL"
     gene            complement(2303..3106)
                     /locus_tag="PKM34_06300"
     CDS             complement(2303..3106)
                     /locus_tag="PKM34_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83235.1"
                     /translation="MKLLLSLTLFFSIVVESIAQVPLNWTRDEINPGEDFTLFQDNSV
                     FTEGEKSLHMQLNKGSVPYLVSDIFYISPGADYTFSVDVLDNDTAGQVKIYADFYDAY
                     GFDIFGEPPVFSADSADWVTISWQGTVPSQAVVGYILIKFYNQPNLYTFTKQANIWLD
                     NVRFIQEGGPNLVANGGFEEWAVGMDDDREEMELTVFPNPADDFIGLKFTDKINRVEL
                     YNSAGQRVRLQEANGTVAGIPVAGLMEGLYLLKIETGIGVVVKKLIIHH"
     gene            3310..3927
                     /gene="udk"
                     /locus_tag="PKM34_06305"
     CDS             3310..3927
                     /gene="udk"
                     /locus_tag="PKM34_06305"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNQ83236.1"
                     /translation="MLVIGIAGGSGSGKTTVVNKIIEGLPPKSVSIMSQDAYYWDNGH
                     LSPEEKKLINFDHPDSIEWELLIHHLDKLIKGHSIEMPRYSYVECARLKETVKIEPKR
                     VIIVEGILILTHPELRNRFNVKVFVDADADDRLTRIIQRDILERGRDVAMVLRHYETY
                     VKPMHLQFIEPSKRYADLIIPQGGENSVAIDLLSSRINWKFNQER"
     gene            3924..4487
                     /locus_tag="PKM34_06310"
     CDS             3924..4487
                     /locus_tag="PKM34_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNQ83237.1"
                     /translation="MILIGDTAVSDDLHLVRFCCHLARCQGACCVAGDAGAPLEEEEI
                     AILEDELEAIKPFMTERGIATVENSGVFDYDIAGKFVTPLVNDGECAFTNFNNGIAYC
                     AIEKACSEGKTRFLKPVSCHLYPVRITKYATFEAVNYHKWNICKPALKLGNKEGIPLY
                     KFLRTALVRKYGESWYRELEKEISSRE"
BASE COUNT         1272 a         1210 c         1226 g         1228 t
ORIGIN      
        1 cctggctttt aaaagcgaag aacttattca gatagccaaa gtaggtattt tcactgcatc
       61 cattctatcg gcactggtcg gaatgatctt cctttactcc tcaaagacaa aaaaataggt
      121 cagattcatc attattattt aattttagat aatgttttcc gatgaatatt ttatgaggga
      181 agccttgaaa gaggcccgga aagcttttga tctcgacgaa gtcccggtag gtgccgtcat
      241 tgtttgcaac gaccggatca tcggccgcgg acataacctt accgagaggc tgaccgatgt
      301 gacggcgcat gccgagatgc aggcttttac cgctgctgcc ggccatctgg gcgcgaaata
      361 ccttgacgaa tgcaccctgt atgtcaccct ggaaccctgt gtaatgtgcg caggggccgc
      421 tttctggaca caaatcggga ggattgtcta cggggcgccc gacgataaaa ggggctacac
      481 caaaaccggc gccggtctgc tgtaccccag gacaacagta acaccgggca ttctcgcgca
      541 ggattgcgct gacttactga tcgaattttt caaatccaaa cggaacgatg caactgaata
      601 agaccaacca gggggcgcag ggtgtcgccg ccggaattgc cgaagtggca tcgctgctct
      661 ggcaaaaggg ctgggcggaa gccagcggag gaaatatttc ggtcaatgtc accgagtttt
      721 atcccaatgt caggattgat ttcaggacgt acccgatgat ccccctgccg gtaaaatatc
      781 cggccattgc ccacaactac atcttcatta ccagcaaagg atccaggatg aggacactgg
      841 caaaagaccc cggttccgga atttgcctgg tcaaaatctc caaatcgggt gaggcgtacc
      901 aggtactgtt cgaagaccct gaaaatcccc tggtcccctc ttccgaactg ccctcccact
      961 ttgctattca caacctcctg gtgaaacaaa aacgggaaga aaaagcagtc gtgcatgccc
     1021 atgtacatga aatggtggcg atgtcgcaca tcagcggatt taataatgaa gcgcggttga
     1081 atgaattgtt gatgggtatg cataccgaaa cggcctattt tatcccggaa ggaatgggct
     1141 atgtgcctct tctggagccc ggatcgcagg agctggccaa agccacctta aaatcgctga
     1201 aagatcataa ggtcattctc tgggaaaagc atggctgcct ggcaacaggc aaggatgtcg
     1261 atgaggcatt cgaccggatc gacctggtgg ctaaagcggc gaagatttac ctgcttgcca
     1321 gccataatcg acatattccc agctgatctc atcccctttg gctttcagca gtagaaagcc
     1381 ttcctcggtg cccaggaacg gcccttccca ccaggctgcc gatacagcgc cagccgtcac
     1441 atagttcgtc ccgaaaacgt gcagagattc ataataatgc agatggccct gtaaaaccag
     1501 cttcaggttg tggccattaa aaagatcgat cacctctttg gaattgacga cgacctcgtt
     1561 tcggtcgttg gctgccgtgg ctccccttag aatttgtgct tcggttgtca gcagcgggat
     1621 gtggaggctt ataatgattg gcgtttcggc tccgatggag gcgagctgtt ccctgagcca
     1681 ggctatctgc acagcatcga cgccaccctc atagccgtcg ccggtgctgt cttttactac
     1741 cgaatccagg ataaatatga tccatccaaa gcggtcgatc cgctgaaatc tcggtccgat
     1801 cctgttttca aacattttct tgccgtactc gggatgattt gtatcgccat ctttcaaaac
     1861 ataccagccg tatatttcat ggttccccat cgtattgaaa acaggcatat tgaactcggt
     1921 catcatcgtt gtgtagatat gatacaggga atcagccctc tcataggtct ggcccaaagc
     1981 atccattacc aggtcgccgc ccgtgatcac gaaatcgggc ttcaattcat tgacctttgt
     2041 aatggccttc cggaagcccg aaatagcgtc tttctcccgc tgcacgtgaa tatcggtcag
     2101 aaaggcgaag gtgaactcat tctgcgcctt ttcttgttgt ggcggttgtt ggttgcagga
     2161 tgtgaagagg atcagtaccc ctgagataag aatgattata aattttgttt tgttcatagt
     2221 tgatgattat tgtttctcac agatttcaca gattgaggac acaaaattca cagatatttc
     2281 cttttggtct gttaaatatt aattaatgat ggatgatcag tttcttcaca acgacaccga
     2341 tgccggtctc aatcttaaga agatatagtc cctccatcag gcctgcaaca gggattccgg
     2401 ccactgtgcc attagcctcc tgaagcctca cccgttgtcc ggcgctgtta tacaattcga
     2461 cgcggttaat tttatctgtg aattttaaac cgataaaatc atcggccgga tttggaaaaa
     2521 cagtcaactc catttcttcc cgatcatcat ccataccgac cgcccattct tcaaatccgc
     2581 cgtttgccac caggttcggg ccgccctcct ggataaacct gacgttatcc agccaaatat
     2641 ttgcctgttt agtaaatgta taaagattag gttgattata gaatttgatg aggatgtaac
     2701 cgacaacggc ctggctcggg acggtcccct gccaggaaat cgtcacccaa tcggccgaat
     2761 ccgctgaaaa cacgggcggt tcgccgaaaa tatcaaatcc gtaggcatcg tagaaatcgg
     2821 catagatctt cacctggccg gccgtatcgt tatccaggac atcgaccgaa aaagtataat
     2881 cggcgccggg gctgatgtaa aagatatccg atacaaggta gggcaccgaa cctttgttca
     2941 actgcatgtg cagcgatttc tcgccttcgg tgaataccga attatcctgg aaaagcgtga
     3001 agtcctcccc ggggttgatc tcatcgcggg tccagttcag cggaacctgg gcgattgatt
     3061 caacaactat tgaaaagaaa agtgtaagtg atagtagcaa cttcattctg aaaggaatta
     3121 tttattcagt aaaaataata taaagtgcga taatgtcaga aggtggtcat tagttcgcta
     3181 cgcgaacacc ataagctcac tgcgttcgcg tcattattat attgatcatc tgtcggcgat
     3241 gatccctcgc cgataagctt ttcaggacat gctgccaact ttttgtattt tagcaaaaaa
     3301 atcagcacca tgctcgtcat aggaatcgcc ggtggatcgg ggtcggggaa aaccaccgtt
     3361 gtcaacaaga tcatcgaggg cttgccgcca aagtcggttt ccatcatgtc gcaggacgcc
     3421 tactactggg acaacggcca tttgtcgccg gaggagaaaa agctgatcaa cttcgaccat
     3481 cccgattcga tcgaatggga gctgctcatt catcacctcg ataagctgat aaaagggcat
     3541 tccattgaga tgccgcgcta ttcttacgtg gaatgtgccc gcttgaaaga aacggttaag
     3601 attgagccca agcgggtgat catcgtcgag ggcatcctga tcctcaccca tccagagctg
     3661 cgtaaccggt tcaatgtgaa ggtatttgtg gatgccgatg ccgacgaccg cctgacccgc
     3721 atcatccagc gcgacatcct ggagcgcggc cgtgatgtcg ccatggtgct gcgccactac
     3781 gagacgtatg tcaaaccgat gcatctgcag ttcatcgagc catcgaaaag gtatgccgac
     3841 ctgatcatcc cgcagggagg agagaacagc gtggcgatcg acctgctgag cagccgcatc
     3901 aactggaaat ttaaccagga gagatgatcc tcatcggcga caccgcagtt tccgacgacc
     3961 tgcacctggt caggttctgc tgccacctgg cgcggtgcca gggggcgtgc tgcgtggccg
     4021 gtgacgccgg cgccccgctt gaagaagagg agatagccat cctggaagac gagttggaag
     4081 cgataaagcc gtttatgacc gaacgaggca tagcaacggt tgagaactcc ggtgtttttg
     4141 attacgatat tgccggaaag tttgtcacgc ccctggtgaa cgacggcgaa tgcgcgttta
     4201 ccaattttaa caacgggata gcttattgtg ccattgaaaa ggcctgcagc gaaggcaaaa
     4261 cccgtttcct gaagcctgtt tcatgccatc tttacccggt caggatcacg aaatacgcca
     4321 cattcgaagc ggtgaactac cacaagtgga atatttgtaa accggctctg aagctgggaa
     4381 acaaagaggg gataccgctt tacaaatttc tgaggacggc cctggtgagg aaatacgggg
     4441 aaagctggta ccgggagctt gagaaggaga tcagctccag ggagtagagg cattacgcga
     4501 ttcggcattc ggcattcggc attttcgatt tgatttggta gtacgacggg taaatccttt
     4561 ggttgttgtt tgtattgctc caacgggtaa atccgttggt gccatattgt gcatctttgg
     4621 aaccgacgga ttcacctgtc ggaagacaat tccatctgga actgatagtg taatggggta
     4681 tttctgtgag actccatctg aatttaatga gactccatct caaattagag atggagtctc
     4741 atgtacgaaa aagttatcaa ccatcccttg acttttgaat atgtcttatt tatatttgtt
     4801 ccatgcattt cgcgttttca tttttaacca aatccaattg atccaaatga ttcggtttta
     4861 cctaatagcg cgtttctgta gagagagaga gagagagaga gagagagagt ttaataaaga
     4921 tccccccgta atgcgg
//
LOCUS       DAOQXS010000186         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17401_length_4929_cov_3.612228, whole genome shotgun sequence.
ACCESSION   DAOQXS010000186 DAOQXS010000000
VERSION     DAOQXS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17401_length_4929_cov_3.612228"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="PKM34_06315"
     CDS             <1..1838
                     /locus_tag="PKM34_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNQ83238.1"
                     /translation="DIYLPYRPKRRTRATIAREKGLEPLAKIIMSRFEQDPYRRAKQY
                     VNAQKGVADEEEALAGARDIIAEWISESQPARARVRKLFLRDGQIYSRIVKGKEIEGV
                     NYRNYYEWNEPVSKIPSHRFLAMMRGEAEGFLKTDVYPPKEKAIETLEDIFIRNATDG
                     CAEQIRKAVNDSYGRLLQPSMENEIKAAAKEKADKYAITVFADNLKQLLMQPPLGQKN
                     VLAVDPGFRTGCKLVCLDKQGNLLHNETIYPHPPENQVKMAEKKILSLVDAYKIEAIA
                     IGNGTAGRETERFIRNIRFDKDLIAVMVNESGASIYSASKIARDEFPDYDVTVRGAVS
                     IGRRLMDPLAELVKIDPKSIGVGQYQHDVNQSDLQQSLEDTVTICVNQVGVEINTASK
                     ELLSYVSGVGPSLAQSIVNFRKENGPFNSRDQFRKVPRFGDKAFEQAAGFLRIRDAVN
                     PLDRSAVHPESYHVVAEMAKRTGASVADLIQNDDIRKKINLNDYVSEKIGLPTLTDIL
                     QELAKPGRDPRQKFDMFEFAKGINSIDDLVPGMVLPGIITNITAFGAFVDIGVHQDGL
                     VHVSQMANRFVRDPKEVVKINQKVMVKVLEVDKERKRINLTMKEI"
     gene            1974..2756
                     /locus_tag="PKM34_06320"
     CDS             1974..2756
                     /locus_tag="PKM34_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ83239.1"
                     /translation="MKSVGIILVFWILAGLVFTGCVREEESIELKIALSKGSPENSYA
                     NYYNWVKSLDSNAVSLDMYDMPLDSALALFKGCDGLIITGGTDINPAWYDQEEDSLRC
                     MTIDHYRDTLEMRLIDSALAWKMPILGICRGHQMLNVYFGGSLIIDIPSDYDTLVSHR
                     CPDFNTCFHPVTLADGSLIAELAGQTAGTVNSNHHQGIKNLAPALKIVAFTEDNLPEA
                     VEWNEPAGKTFLMGVQWHPERLGNDNPLSKEIGMRFLRECRK"
     gene            complement(2782..3363)
                     /locus_tag="PKM34_06325"
     CDS             complement(2782..3363)
                     /locus_tag="PKM34_06325"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNQ83240.1"
                     /translation="MREELTKNRCGWISDPLMQEYHDEEWGVPLHDDRKIFEFMVLDA
                     FQAGLSWKTILNKRENFRRAFDNFIPEKIARYDEAKYEELLKDEGIIRNRSKIRATIS
                     NANAFLAVQKEFGTFDRYIWSFVGHTTIRNNWKSMQDIPAKTEVSDRMSKDLADRGFK
                     FVGSTICYAFMQAAGMVNDHITGCFRYKELQDK"
     gene            complement(3368..4084)
                     /locus_tag="PKM34_06330"
     CDS             complement(3368..4084)
                     /locus_tag="PKM34_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNQ83241.1"
                     /translation="MKLLRYLFIIGLLMASVSSADAQRFLGFAGAGMNLSQVDGDEVV
                     GYNKAGGHLSLGVSLPIRKKWDVTLETVFSQKGAREKAQYIHTITDSTVVPVKTQTYT
                     GEYNLKLNYVEVPLTAHYTDKERYTVGMGFSYGRLVNSKEIEHGGNIAPYSDTVKFRY
                     NDWNVIFDLQFRIWKQLKGNIRFNYSIFPIRERIYKDVIYGIRDPYERKQYNHLFTVR
                     LIYVFNEKYNKNTQPEELTQ"
     gene            complement(4148..>4929)
                     /locus_tag="PKM34_06335"
     CDS             complement(4148..>4929)
                     /locus_tag="PKM34_06335"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNQ83242.1"
                     /translation="IYDATDQATGRPIRFMRINNESSSAEFHDSREPVFPYMRFYRLA
                     EHFTPGFRSALILGGAAFSYPKYFLQQYPQATLDVVEIDPELTELARQHFNLRDDPRM
                     RIFHEDARTFLNRSEYRYDVIFGDTYKSLYTLPWHLTTREAAQKTYEMLNEGGCVILN
                     IISSIGGESSLFLQAETATYRDVFPHVYIFAADNPVDEDVLQGTLLVALKTSSKSGLT
                     SSDPELSAMLKNNVTHLIAAGVPVLTDDWAPVDFYTNKSIR"
BASE COUNT         1285 a         1185 c         1205 g         1254 t
ORIGIN      
        1 aggatattta ccttccctac cggcccaagc gcagaacccg tgcaaccatc gccagggaaa
       61 aagggctcga acccctggct aagatcatca tgtcgcgctt tgaacaagat ccgtaccgcc
      121 gtgcaaagca gtatgtcaat gcgcagaaag gagtagcgga tgaagaagag gcactggcgg
      181 gcgcccgcga tatcatcgcc gaatggatca gtgaaagcca gcccgcccgg gcccgggtga
      241 ggaaattgtt cctcagagac ggacaaatct attccaggat tgtcaaaggg aaagaaatag
      301 aaggtgttaa ctatcgtaat tactacgaat ggaacgagcc ggtatcaaaa atcccttccc
      361 ataggttcct tgccatgatg cggggagaag ccgagggatt cctgaaaaca gatgtgtacc
      421 cccccaaaga aaaagcgatc gaaacactcg aggatatttt cataagaaac gcgactgatg
      481 gttgtgcgga acaaatccgt aaggcggtaa atgatagtta tggccggttg ctccaacctt
      541 cgatggaaaa cgaaattaaa gccgccgcca aagagaaagc ggataaatat gccattaccg
      601 tttttgctga taacctgaaa caattgctta tgcaacctcc gctcgggcaa aaaaatgttt
      661 tggccgtcga tccgggattc aggacagggt gcaaactcgt ttgcctcgat aagcagggca
      721 accttcttca taatgaaacg atttaccccc atccgcccga gaaccaggtg aaaatggcgg
      781 agaaaaaaat cctgtcgctg gtggatgcat ataaaatcga agcgattgct atcggaaatg
      841 gtactgccgg tcgtgagacc gaacgattta tccggaatat ccgttttgat aaagacctga
      901 tagccgtcat ggtgaacgaa agcggcgctt cgatctactc ggcgtcgaaa attgccaggg
      961 atgagttccc cgattatgac gtcaccgtca ggggtgcggt ctcgatcggc cgtcgcctga
     1021 tggatccgct ggccgaactg gtaaagatcg acccgaaatc gatcggggta gggcagtatc
     1081 agcacgacgt caaccagtcc gatctccagc aaagccttga ggatacggta acgatctgcg
     1141 taaaccaggt cggggtcgaa atcaataccg ccagcaaaga actattgtca tatgtttccg
     1201 gagtcggccc ttcactggct cagagcattg tcaatttcag aaaagagaat ggcccgttca
     1261 actctcgtga ccagttccgg aaagtccccc gcttcggcga taaagcgttc gagcaggctg
     1321 ccggattcct ccggatccgc gatgcggtga acccgctgga tcgaagcgcc gtccatccgg
     1381 agagctacca tgtggttgcc gaaatggcga aacgtaccgg tgcttccgtt gccgatctga
     1441 tccaaaatga tgatattcgg aaaaagatca atctcaacga ttatgtcagt gaaaagattg
     1501 gccttccgac cctgacggat atcctgcagg aactggccaa accgggacgt gatccgaggc
     1561 aaaaattcga catgttcgaa ttcgccaaag ggatcaacag tatcgacgac ctggtgccgg
     1621 gtatggtact ccctggaatc atcaccaata taacggcttt cggggccttc gtggatatag
     1681 gagtccacca ggacggcctg gtacatgtca gccagatggc aaaccggttt gtacgcgacc
     1741 cgaaagaggt ggtgaaaatc aatcagaaag ttatggttaa ggtgctggag gtggataagg
     1801 agagaaaacg aatcaacctc accatgaagg agatttgatg atattttgat tttttgattt
     1861 tttgatattg tgaaattatg atattgtgaa attatgatat tgtgaaattg tgatattgtg
     1921 aaattgtgag cttgtgaagt tgtgttctta tgaaatcttg atcttctgat attatgaaaa
     1981 gcgtaggtat tattttggtt ttttggattc ttgcgggtct ggtatttacg ggatgcgtta
     2041 gggaggagga gtcaattgag ttaaaaattg ccctttcgaa aggcagcccg gagaattcct
     2101 atgccaatta ttacaactgg gttaagtcgc ttgattccaa tgctgtgagt cttgatatgt
     2161 acgatatgcc ccttgattct gccctggcgc tttttaaagg atgcgatgga ttgatcatta
     2221 ccgggggaac cgatattaat ccggcctggt atgaccagga agaggactcg ctccggtgca
     2281 tgaccatcga ccactaccgc gatacgctcg aaatgaggct gatcgattcg gccctggcct
     2341 ggaaaatgcc catcctgggc atctgccggg ggcatcagat gctcaatgtc tatttcggcg
     2401 gcagcctgat catcgacata ccctccgatt atgataccct ggtcagccac cgatgccccg
     2461 attttaatac ctgcttccat ccggttaccc ttgccgacgg ctcactgata gcggaacttg
     2521 caggccagac ggccggaaca gtgaacagca atcaccacca gggaataaaa aacctggcgc
     2581 ctgcgttgaa aatcgtggct ttcactgaag ataacctgcc ggaagccgtg gaatggaatg
     2641 aacctgccgg caaaaccttc ctgatgggcg tgcagtggca ccctgaaaga ttggggaacg
     2701 ataatcctct ctcgaaagaa ataggaatga gatttctgcg ggaatgcagg aaataaccct
     2761 ggtcactaat ccctgaataa tttatttatc ctgtaattct ttataacgga agcacccggt
     2821 aatatggtcg ttgaccatgc ccgccgcctg catgaaagcg taacatattg tcgagccgac
     2881 aaatttgaat cccctatccg ccaggtcttt gctcatcctg tccgatactt ctgtttttgc
     2941 aggtatatcc tgcatcgact tccagttgtt ccggatagtt gtgtggccga caaaagacca
     3001 gatatatcgg tcaaaagtcc caaactcttt ctgaactgcc aggaaagcat tcgcattgga
     3061 tatcgtggcc cggatctttg acctgtttct gattatccct tcatctttta acaattcctc
     3121 atatttggct tcatcatacc tggctatctt ctccgggata aagttatcaa aggcccgcct
     3181 gaaattctcc ctcttgttta aaatggtctt ccagcttaaa cctgcctgaa atgcgtccag
     3241 taccatgaac tcgaatatct tgcgatcgtc atgtaatgga actccccact cctcatcatg
     3301 atactcttgc atcagcggat cactgatcca gccgcacctg tttttagtca actcctctct
     3361 catccgatca ttgggttaat tcttcgggtt gagtattctt attgtacttc tcattgaaaa
     3421 cataaatcag ccgaaccgta aacaagtgat tgtactgctt gcgttcgtat ggatccctga
     3481 tgccatagat gacatcttta taaattcgct ccctgatggg gaaaatggag taattgaacc
     3541 ggatattgcc ttttaattgt ttccagatcc tgaactgcag gtcgaatatg acattccagt
     3601 cgttatacct gaatttgacc gtatccgaat agggtgcaat gttgccccca tgttcgattt
     3661 cttttgaatt gaccagcctt ccgtaggaaa aacccatacc cacagtgtat ctttctttgt
     3721 cggtataatg ggcagtgagg ggtacctcga cgtaattaag ttttaggtta tattcgcccg
     3781 tataagtttg agttttaact gggactaccg tactgtcggt aatggtgtga atatattgcg
     3841 ctttttcccg ggcccctttc tggctgaaga cggtttcgag ggtaacatcc cacttcttac
     3901 ggatcggtag agatacaccc agcgataaat gcccccccgc tttgttgtaa ccgaccactt
     3961 catcgccatc cacctgcgac aggttcattc cggctcctgc aaaacccagg aaccgctgtg
     4021 catcagccga agaaaccgat gccattagca gaccaatgat gaataaatat cggagtaact
     4081 tcattttccc tggatttctt ctacaacgga atattcaggc agtttatttt ttcgatacat
     4141 catttgttta ccggatgctt ttgtttgtgt aaaaatcaac aggggcccaa tcatccgtca
     4201 gtaccggtac acctgcggca atcaggtgtg tcacattatt tttcagcata gccgacaatt
     4261 cgggatcgga gctggtcagc cctgacttcg atgaggtttt taaggcaacc agaagtgtgc
     4321 cctgcaatac atcttcatcc accgggttat cggcagcaaa gatataaaca tgcgggaaaa
     4381 catcgcggta agtagctgtt tctgcctgca ggaaaagact cgactctccg ccgatggatg
     4441 agatgatatt cagaatcaca cacccgcctt cattcagcat ttcgtaagtc ttttgcgccg
     4501 cttcccgggt ggtcaggtgc cagggtaatg tatacagcga tttatatgtg tcgccaaaaa
     4561 tgacatcgta acggtattcc gaccggttca ggaaggtcct ggcatcttca tggaaaatcc
     4621 gcatgcgcgg atcatcccgc aggttgaaat gctgccgggc cagttccgtc agctcgggat
     4681 cgatctccac tacatcaaga gtggcctgcg gatattgctg aaggaaatat ttcgggtaac
     4741 tgaatgccgc tccgcctaat atgagcgctg acctgaaacc gggggtgaaa tgttcggcca
     4801 gccggtaaaa ccgcatgtaa gggaaaaccg gttcccggct gtcgtgaaac tctgccgacg
     4861 aactctcgtt gttgatccgc ataaaccgga tgggcctgcc ggtggcctgg tccgtggcat
     4921 cgtaaatcc
//
LOCUS       DAOQXS010000187         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17428_length_4926_cov_3.931842, whole genome shotgun sequence.
ACCESSION   DAOQXS010000187 DAOQXS010000000
VERSION     DAOQXS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17428_length_4926_cov_3.931842"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKM34_06340"
     CDS             <1..278
                     /locus_tag="PKM34_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNQ83243.1"
                     /translation="LNRFRKKNVDFIRKFMAGVKLTELDSTGRLLIPKDLFNFAAIKQ
                     DVVMASVVNKIEIWDKDEYEKAVSYDAEEFANLAEEVMGDNDVEPIE"
     gene            278..1162
                     /gene="rsmH"
                     /locus_tag="PKM34_06345"
     CDS             278..1162
                     /gene="rsmH"
                     /locus_tag="PKM34_06345"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNQ83244.1"
                     /translation="MYHIPVLLKESVDGLDIRPDGIYVDATFGSGGHSGKILEKLVIG
                     KLFAFDADRDTLANAPEDARFELIGHNFRYLKNFLRIHGITRIDGILADLGISSWQID
                     QPDRGFSTRFSGKLDMRMDREQDKDAWQVVNGYSLEDLTIVLKKYGEVENAFRLSQEI
                     ILSRPVNTTEEFKSLVSRRAPKGKENQYLAKVFQAIRIEVNDELEALKELLQHGTDLL
                     HPGGRFAVISYHSLEDRLVKNYFKTGNFEGNPVKDFYGNLIAPLAPVTRKAIIPSASE
                     QSVNSRSRSAKLRIAKKI"
     gene            1164..1571
                     /locus_tag="PKM34_06350"
     CDS             1164..1571
                     /locus_tag="PKM34_06350"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNQ83245.1"
                     /translation="MDSRGEKRSATFFKLPGKEVFSRRHIGQSIHSILDGTFLTRERF
                     MRALPFIIFLIILGIMYITNIFHVEKTKRQIDDLEEELRELRYEYITSRSRVMFESKS
                     SEIARKLKDTGISESMHPPKKIIIGEKDKISMP"
     gene            1568..3691
                     /locus_tag="PKM34_06355"
     CDS             1568..3691
                     /locus_tag="PKM34_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNQ83246.1"
                     /translation="MKDIKRDILWRVYLVYFGVLVFALVIIYRAAYLQFSMKDELLEK
                     AKKQEVKYFPIEALRGNILARDGSLLASSIPDFEIRMDLSPNVVQPEIFNAKIDSLSM
                     LLAGLFKDKSSLDYKSDLLRARADGNRYFLVKNHVKYDELKKLKRFPIFRLGKYAGGL
                     IVIRKTTRELPYKELARRTIGFENQTESLFVGLEGAYSEDLKGHEGLQLKRRISQGEW
                     IPVNDNPEVAPRDGRDIITTIDVNIQDLAESALARHLEEHKAFQGCAILMEVSTGEIR
                     AIANLRYDPKDGKFKEIYNYAVGETIEPGSTFKLPSLIAALETGKIRLSDQYYVGNGR
                     VQYHNRWMLDSHRPESDHMSVQRIFEESSNVGVSKIITTLFGAHPEEYVSLLRKMSLG
                     QQLGVDIPGEGKPYLKDPSDKQHWYGTSLPWMSIGYELTLTPLQILTLYNAVANNGVM
                     VKPIFVSEIREGGKTIRKNEISVLNPSICSPSTLTQVKYLLEGVVENGTGSVLKDSTY
                     RIAGKTGTALVADGEKGYSEKKYNASFVGYFPADNPKYSCIVVVNRPEAGKIYGGAVA
                     APVFKEIADKVYATQMDIHDQTEKTEYGKIHLPDRGKGNYQDLKTSLVSLSYPVMMPS
                     QTPEWVQMDSSVVGIVLKPAYYGIDTIPDMIGMTGKDAVYMMEKAGLKPVIHGKGLVS
                     EQSLLAGTWLEKGSRIELFLSDTIR"
     gene            3688..>4926
                     /locus_tag="PKM34_06360"
     CDS             3688..>4926
                     /locus_tag="PKM34_06360"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNQ83247.1"
                     /translation="MKKLSDILYKAGVVRVVGNPHARIGEIRFDSRLVGKGDLFVAVR
                     GTQADGHAFIGKAIESGAAVVVCEEMPGTLIPGITYAQVTDCSFALGVMASNYFDNPS
                     EELTLVGVTGTNGKTTIATLLYQVFTGLGYGCGLLSTIRNVIMGKVIAATHTTPDPLA
                     LNALLRQLADEGGQYCFMEVSSHAVAQNRIAGLNFAGGIFTNLTHDHLDYHKTFKDYL
                     LAKKKFFDLLPSRAFALVNKDDKNGMVMVQNTTARIRTYAVKSPADYKCRITENLLEG
                     LQLTINETEIYCRLTGEFNAYNLTAVFGTALLLQQPAENVLTFLSSVPPVEGRFDTLH
                     SDNGITAIVDYAHTPDALKNVLTTIEEIRTRNEKLITVVGAGGDRDRSKRPLMGGICA
                     AKSDLVILTSDNPRSEEPEEI"
BASE COUNT         1333 a         1243 c         1304 g         1046 t
ORIGIN      
        1 aactcaaccg tttccgaaag aagaacgtgg atttcatccg gaaattcatg gccggcgtca
       61 aactgacaga acttgactca acgggcaggc tgctgatccc gaaagacctg ttcaatttcg
      121 ccgcgataaa gcaggatgtg gtgatggcat cggttgtcaa taaaattgaa atctgggata
      181 aggacgagta cgaaaaagcg gtttcgtacg acgctgagga attcgcaaac ctggccgaag
      241 aggtcatggg cgataacgat gttgaaccca tagagtgatg taccatattc cagtcctgtt
      301 gaaagaatcg gttgatggcc ttgacatacg tcctgacggg atttatgtcg acgccacttt
      361 tggcagtggc ggccactccg gcaagatcct ggaaaagctg gtcatcggga agcttttcgc
      421 cttcgatgca gacagggaca cactcgccaa cgccccggaa gacgcgcggt tcgaactgat
      481 cggccataat ttcaggtacc tgaagaattt cctccggatc catggtatca cccggatcga
      541 cggtatcctg gccgacctgg gtatctcctc ctggcagatc gatcagcctg acaggggatt
      601 ttccacccgc tttagcggaa agctcgatat gcggatggac cgggagcagg ataaagatgc
      661 atggcaggtt gtgaacggtt actcccttga ggacctgaca atagtcctga aaaaatacgg
      721 ggaagtagaa aatgccttca ggctttcgca ggaaatcatc ctcagccgtc cggtcaacac
      781 cacggaggaa ttcaagtcgc tggtttcacg gcgggctccc aaaggcaagg aaaatcaata
      841 cctggccaaa gtcttccagg ccatccggat cgaagtaaac gatgaactgg aagccctgaa
      901 agagttatta cagcatggaa cagacctgtt acacccgggc ggacgtttcg ccgtgatctc
      961 ctaccacagc cttgaagaca gactggtgaa gaattacttt aaaacgggca atttcgaagg
     1021 caacccggtg aaagatttct atggcaacct gattgccccc ctggcgcctg taacgcgcaa
     1081 agccatcatc ccttcagcct cagaacaatc ggtcaacagc cggtcgagaa gcgctaaact
     1141 cagaattgca aaaaagatct gatatggata gcaggggaga aaaacgatcg gcaacattct
     1201 tcaaattacc cggcaaggag gtattcagcc ggaggcatat cggccagtcg attcattcca
     1261 ttctcgatgg caccttctta acaagggaaa ggttcatgcg ggcccttcct ttcatcatct
     1321 ttcttatcat attgggtatc atgtatataa ccaatatttt tcatgtagag aagaccaaac
     1381 gtcagatcga tgacctggaa gaggaactga gagagctccg gtacgaatac attacatccc
     1441 gcagccgggt catgtttgag agcaaatcat cggaaattgc caggaagctt aaggatacag
     1501 ggatcagcga atcgatgcat ccgccgaaaa agatcatcat cggtgaaaaa gacaaaatca
     1561 gcatgccatg aaagatataa aaagggacat actatggcgg gtttacctgg tttacttcgg
     1621 ggtactggtt tttgcgctgg taatcatcta ccgtgcggct tatcttcaat tttccatgaa
     1681 ggatgagttg cttgaaaaag caaaaaaaca ggaggtgaaa tacttcccca tcgaagcctt
     1741 acgcggaaac atcctggcgc gcgacggcag cctgctggct tcatcgattc ccgattttga
     1801 aatccgtatg gatctttcgc ccaatgtcgt tcagccggaa atattcaatg caaagatcga
     1861 ctcgctttcg atgctgctcg ccgggttatt caaagataaa tcgtcactgg attacaaatc
     1921 agacctgctt cgtgcgcggg ccgacggaaa ccgctatttc ctggtcaaaa accatgttaa
     1981 atatgatgaa ttaaagaaac tgaagagatt tccgatcttc cgcctgggta aatacgccgg
     2041 gggattgatc gttatccgga agacgacacg tgaattgcct tacaaagaac ttgcgcgtcg
     2101 gaccatcggt tttgaaaacc agaccgaaag cctttttgtg gggctggaag gggcctacag
     2161 cgaggacctc aaaggccatg aaggcctgca actgaaacga cggatcagcc agggggaatg
     2221 gatcccggtg aacgacaatc cggaagtagc ccccagggat ggacgcgata ttattacgac
     2281 gatcgacgtg aatatacagg acctggccga atcggccctg gcgcgccacc tggaggagca
     2341 caaagcgttt cagggctgtg cgatcctgat ggaggtcagc accggtgaga tccgggccat
     2401 cgccaacctt cgctacgatc cgaaagacgg aaagttcaag gagatctaca actatgccgt
     2461 cggggaaacc atcgaaccgg ggtccacttt caagctaccc tccctcatag cggcacttga
     2521 aaccggcaag atcaggcttt cggaccagta ttacgtcggc aacggccggg ttcagtacca
     2581 taatcgctgg atgctcgatt cgcatcgtcc agaatcggat cacatgagcg ttcagcgaat
     2641 attcgaagag tcatccaatg tcggcgtatc caaaatcatc acaaccctgt tcggtgctca
     2701 cccggaagaa tatgtttcac tgctccggaa gatgtcgctc ggtcagcagc tcggagtgga
     2761 tattccggga gaaggcaaac cgtatctgaa ggatccctcc gacaagcaac attggtacgg
     2821 taccagtctg ccctggatgt cgatcggata tgaactgacc ctcaccccat tacagatcct
     2881 gaccctttat aatgccgtgg cgaacaatgg cgtaatggta aaaccgatct tcgtcagcga
     2941 aatcagggaa gggggaaaaa ccatccggaa aaacgaaata tcggtgctca atccctctat
     3001 ctgctcgccg tcaaccctga cacaggtcaa gtacctcctg gagggtgtcg tggagaacgg
     3061 gacgggcagt gttttgaaag actcaacgta ccggattgcc ggaaagacag gcacagcgct
     3121 ggttgccgat ggcgagaagg gctattccga aaagaaatac aatgcctcat ttgttggcta
     3181 cttcccggcc gacaatccca agtattcctg tatagtcgtt gtaaaccggc cggaagcggg
     3241 gaagatatac ggcggagctg tggcagcccc tgttttcaag gagattgccg ataaagtcta
     3301 tgccacgcag atggatatcc acgaccagac agaaaaaacg gaatacggaa agattcacct
     3361 tcccgatcgg ggcaaaggca attaccagga cctgaaaaca agcctcgtca gtctttcata
     3421 cccggtcatg atgccctcac agacacccga atgggtacaa atggacagtt ccgtggtggg
     3481 tatcgtactg aaacccgcct attacggaat tgacacgata ccggatatga tcggcatgac
     3541 cggaaaagac gccgtctaca tgatggaaaa ggcaggttta aaaccggtca tccacgggaa
     3601 agggctggta agcgaacaat cattgctggc aggaacctgg ctggagaaag ggagccggat
     3661 tgaattattt ctttcagata cgatcaggtg aagaaactga gcgatatatt gtacaaggcc
     3721 ggtgtggtca gggtcgtagg aaacccgcat gcccggatcg gtgaaatacg cttcgattcg
     3781 aggttggtcg gaaaggggga tcttttcgtg gcggtcaggg gaacgcaggc cgacggtcac
     3841 gctttcatcg gcaaggccat tgaatcgggc gctgccgtgg tcgtttgcga agagatgccc
     3901 gggacattga tacccggaat aacatatgcc caggttaccg actgcagttt tgccctgggg
     3961 gttatggctt ccaattattt tgataatcct tccgaagaac ttacgctggt aggcgtgacg
     4021 ggtaccaacg gcaaaaccac gatcgctacc cttctgtacc aggtatttac cgggctaggc
     4081 tatggctgcg gcctgttatc gacgattcgg aacgttatca tgggcaaggt gatcgctgcc
     4141 acccatacga cgcccgatcc gctggccctg aacgctttac tcaggcaact ggccgatgaa
     4201 ggcgggcaat actgcttcat ggaggtgagt tcccatgccg tcgcacaaaa ccgcatcgcc
     4261 gggctgaact ttgccggggg cattttcacg aacctgactc acgatcacct ggattaccat
     4321 aaaacattca aagattacct gctggccaag aagaaattct tcgacctcct cccctcccgg
     4381 gcttttgcgc tggtcaacaa agacgacaag aacggaatgg tgatggtaca gaataccact
     4441 gcacggatca ggacctacgc cgttaaatca ccggccgatt acaaatgccg gatcacagag
     4501 aacctgctgg aagggctgca gctcacaatt aacgaaacgg aaatttactg caggctgacg
     4561 ggggaattca atgcctacaa cctgacagcc gtcttcggca cggcactcct gctacaacag
     4621 ccggccgaaa atgtgctgac atttctcagc tcggtgccgc cggtagaagg ccgtttcgac
     4681 actcttcact cagataacgg catcacggcc attgtcgatt acgcccatac acccgatgca
     4741 ttgaaaaatg tgctgaccac catcgaagag atccggaccc gcaatgagaa gctgatcact
     4801 gtggtcgggg caggcggtga ccgtgaccgc tcgaagcggc ctttgatggg aggcatctgc
     4861 gcagccaaaa gcgatctggt gattctcact tcagacaatc cgcggtcgga ggagccggaa
     4921 gagatc
//
LOCUS       DAOQXS010000188         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17467_length_4918_cov_3.909932, whole genome shotgun sequence.
ACCESSION   DAOQXS010000188 DAOQXS010000000
VERSION     DAOQXS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17467_length_4918_cov_3.909932"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PKM34_06365"
     CDS             complement(<1..1049)
                     /locus_tag="PKM34_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNQ83248.1"
                     /translation="MEAKFSQRVKDVLTYSREEAIRLGNNYIGLEHLLLGILREGEGL
                     AVQLMRYFGMDLNEIRKMIEKAVASPEETLHHTDNLPLVRQAERALKITYLEAKMFNS
                     ELIGTEHLLLSILRDEENIVTKFFEKNGIDYDLVREELKKMHEVKGSDDKPAPRNELP
                     GDPQDEESEEAGRMFGSTTRKQSDSKSKTPVLDNFGRDITKAAEEGRLDPIVGREKEL
                     ERIAQILSRRKKNNPILIGEPGVGKSAIAEGLADRIVKRKVSRALFDKRIISLDLASL
                     VAGTKYRGQFEERMKAVLNELEKNPDIILFIDEIHTIVGAGNASGSLDASNMFKPALA
                     RGELQPGTGGRHDRER"
     gene            1382..3919
                     /gene="gyrA"
                     /locus_tag="PKM34_06370"
     CDS             1382..3919
                     /gene="gyrA"
                     /locus_tag="PKM34_06370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNQ83249.1"
                     /translation="MNDLNDGALSPENKIIKVNIESQMKSAYIDYSMSVIVSRALPDV
                     RDGLKPVHRRVLFGMLDLGVLSNRPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQE
                     WSLRYPLIDGQGNFGSIDGDNPAAMRYTEARLRKIAEELLEDIDKNTVDFQLNFDDSL
                     EEPKVLPAKIPNLLVNGASGIAVGMATNMPPHNLAQVIDGTIALIDNRDITIEELMQY
                     IKAPDFPTGGIIYGYEGVREAFETGRGRIVVRGEAKIEPTPGGKEKIIVTSIPYMVNK
                     AEMIRRTAEIINDKKIEGIVDIRDESDRNGLRIVYELKRDAVANVILNKLYQMTALQT
                     AFNVNNVALVHGKPKTLNLKELIHYFVEHRHEVVIRRTRYDLDQAEKRAHILEGLLIA
                     LDHLDEVIALIRASRTPDEARDGLMREFQLTEIQAKAILEMRLQRLTGLERDKIKLEY
                     EELQKQIAYLRQVLADESLRMKIIKDELTEIKDKYGDEPRTTIEYTAEDFRMEDVIPE
                     EEVVITISHLGYIKRTPLAEYRRQNRGGRGARGSDLRGEDFLEHLFVANTHNYMLLFT
                     EKGKCFWLRTYEIPEGAKNSKGRAIQNLINIETDDKVRAFIKIKDLTDENYLSSNYII
                     LCTQRGIIKKTTLEAFSRPRQAGIIAITVNEGDQLLAAKLTNGTNEVMLALRSGRAIR
                     FNESNVRPMGRNAAGVKGITPGNSNDEVIGMICVETENFDVLVVSENGYGKRSSLADY
                     RITNRGGKGVKTINITQKTGSLIAIKEVSDSDDLIIINKSGILIRLAVKELRVMGRAT
                     QGVRLINLRDEDAIASVAKVEVDESETDDADYDIDPGSEPENGETTE"
     gene            3998..>4918
                     /locus_tag="PKM34_06375"
     CDS             3998..>4918
                     /locus_tag="PKM34_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83250.1"
                     /translation="MKNLILTVTAMMLVAVVFGQTNKRTSAFNYNNYGKLDLAKEAID
                     EAAQHEKTIMDAKTWFYRGNIYYNIGVSLDSNYRKLDPDPFGVSLKSYLKAKELDVKG
                     EFKENIDQNIVVISQGYFNMGVFYYNEQNYPLAAKNFEQAYEVSKIANQLDTTALFNA
                     AISARDAHDRATAKKYYLEIIKMNVSNPVVYDSLANIYKAEGDTAMALQTIQQGRQVF
                     KEDLNLVIAETNIYLQTNQQDKALANLETAAKLDPTNPTVFWASGTIYDQLGEQDKAI
                     KAYETAIQLKPDYFVAYFNLGILYFNQATEV"
BASE COUNT         1445 a         1084 c         1187 g         1202 t
ORIGIN      
        1 cgttcacggt cgtggcgacc accggtcccc ggctgcagtt cgccacgggc gagagccggt
       61 ttgaacatat tggatgcgtc gagagaaccg gaagcgttac cggccccaac gatcgtatgg
      121 atctcatcga taaacaggat tatatccggg ttcttttcca attcattaag cactgctttc
      181 attcgttctt caaattgtcc gcgatacttg gtaccggcta ccagagaagc cagatcaagt
      241 gaaatgatgc gtttgtcgaa cagtgcccgc gatactttcc ttttcacgat gcggtcggca
      301 aggccttcgg ctatcgcaga tttgcccacg cccggctctc cgatcaggat cggattgttc
      361 tttttgcgcc ggctgagtat ctgggctatc ctttccagtt ccttttcacg gccaacgatt
      421 gggtcaaggc gtccttcctc ggcagctttg gttatatccc ggccaaaatt atccagcacc
      481 ggtgtctttg atttcgaatc ggattgtttc cgtgtggtac ttccgaacat cctgccggct
      541 tcttctgact cttcatcctg aggatcaccc ggcagttcat tccggggagc gggtttatcg
      601 tcagatcctt tcacttcatg cattttcttc agctcctccc tgaccaggtc gtaatcgatc
      661 ccattctttt cgaaaaattt tgtgacgata ttttcttcat cccgtagaat ggaaagcaga
      721 agatgctcgg tgccaattaa ttcgctattg aacattttgg cttccagata ggtgatcttc
      781 agggcgcgct cagcctgtcg gaccagcgga agattatccg tatgatgcaa tgtttcttca
      841 ggactggcga ccgctttttc aatcattttt ctgatctcat tcagatccat cccgaaatac
      901 ctcatcaatt ggaccgcaag gccttcgcct tctcttaaaa tacccagtaa caggtgttcc
      961 agtccgatat aattattacc aagacggatc gcttcttcac gactgtatgt cagaacatct
     1021 ttgaccctct gggaaaattt tgcttccatt ctttaaatta tgaaacgatt cgacaaaatt
     1081 agctaaaatc agcccattaa cataaaccgg accatcatta tacctctgtc agttatctcc
     1141 ccggaaatgt tatacggata aaataaacat cggttatgct ataaagttca tccgtaaaac
     1201 aatactttca accttaaaca aaactactat tcaaactttg aagttgattt ataagttatg
     1261 aataatataa acagaatcct gttagtaata aatagtaata aaaataggta ccccaggtga
     1321 tttcggcaat gaaaaattag taccttcgca tcatagcaga agataattta aaatgttgat
     1381 tatgaatgat ttaaatgatg gcgcattatc acctgaaaac aaaataatta aagtcaatat
     1441 cgagagccag atgaaatcgg cctacatcga ttattcgatg tcggttattg tatccagggc
     1501 tcttcccgat gtcagggacg gattgaaacc ggtacacagg agggttttat tcgggatgct
     1561 ggatttgggc gtcctttcta acaggcctta caagaaatca gcaagaattg tcggggaggt
     1621 attaggaaag taccatccgc atggcgactc atcggtttat gacgccatgg tgagaatggc
     1681 acaggaatgg tcattgagat acccgttgat cgacgggcag ggaaacttcg gatcaatcga
     1741 cggtgataac cctgctgcca tgaggtatac agaagcacgt ctgcgaaaaa ttgcggaaga
     1801 acttctcgaa gacatagata aaaacacggt tgattttcag ctgaattttg atgattcgct
     1861 ggaggagccc aaagtattgc cggctaaaat cccgaacctg ctggtaaacg gcgcttccgg
     1921 gattgctgtc ggtatggcaa ccaatatgcc ccctcacaat cttgcccagg tcatagacgg
     1981 gaccattgct cttattgata accgcgacat cacgattgag gagctgatgc aatatatcaa
     2041 agcacctgat tttccaaccg ggggtatcat atatgggtac gaaggtgtca gggaagcttt
     2101 tgaaaccggc cggggccgga ttgtcgttcg gggcgaagcg aaaatcgaac ctacacccgg
     2161 cgggaaggaa aaaatcattg tgaccagtat cccatacatg gtcaacaaag cagagatgat
     2221 ccggcggacg gcagaaatca tcaatgacaa gaagatagaa ggcattgtcg acatcaggga
     2281 cgaatcggac cggaacggcc tccggattgt ttacgaactg aagagagatg cggttgccaa
     2341 cgtgatattg aacaagctgt accagatgac agccctgcaa acagcgttca atgtcaacaa
     2401 tgttgccctc gttcacggta aacccaagac cctcaacctg aaggaactga ttcactactt
     2461 tgtcgagcac aggcatgaag tggtgatcag aagaacaagg tatgatcttg accaggctga
     2521 aaagcgcgct catatcctgg aaggattgct gattgcacta gaccacctgg atgaagtcat
     2581 cgccctgatc cgtgccagcc ggacgccgga tgaagccagg gatggactga tgcgtgaatt
     2641 tcagcttacg gagatacagg ccaaagccat ccttgagatg aggctgcaac gtctgacagg
     2701 ccttgagcgg gataaaataa aacttgaata cgaggaactt cagaaacaaa tcgcctattt
     2761 acgccaggtc cttgccgacg aatcgctccg gatgaaaatc attaaggatg agctgaccga
     2821 aatcaaggat aaatacgggg atgagccgcg tacgacgatt gaatatacgg cagaggactt
     2881 ccggatggaa gatgttatcc cggaagaaga ggtggtcatt accatttctc atcttggcta
     2941 tataaaacgc acgccactgg ccgaataccg gcgtcagaac aggggtggga ggggagcccg
     3001 gggcagtgac ctgagagggg aggacttcct cgaacactta tttgtcgcta atacccataa
     3061 ttatatgctg ctcttcaccg aaaaggggaa gtgtttctgg ttgaggacct atgaaattcc
     3121 tgaaggggcg aagaacagca aaggaagggc gatccagaac ctgatcaaca tagagaccga
     3181 cgataaggta agggccttta taaagattaa agatctgacg gatgagaact acctgtcttc
     3241 caactatata atactttgca cccaacgcgg aatcatcaaa aaaacaacgc tggaggcgtt
     3301 ttcgaggcca cggcaggccg gtattatcgc tatcacagtc aacgaaggcg accagttgct
     3361 ggcagccaag ctgaccaacg gcaccaatga agtgatgctg gctttgagga gtgggcgcgc
     3421 catccggttt aatgaaagca atgtcaggcc aatgggtcga aatgctgccg gtgtcaaggg
     3481 tataacccca ggaaatagca acgatgaggt tattggtatg atatgcgtgg aaaccgagaa
     3541 ttttgacgtg ttggttgtat cggagaacgg atacggcaaa aggtcgagcc tggccgacta
     3601 caggataacc aaccgtgggg gcaaaggtgt taaaactatc aatatcacgc aaaaaaccgg
     3661 aagcctgatt gcaattaaag aggtgagcga ttccgacgac ctgatcatta tcaataaatc
     3721 gggcatactt atccgattag ctgtaaaaga gttacgggta atgggcaggg caacccaggg
     3781 agtaaggctg atcaatctcc gtgacgaaga cgccattgct tctgtggcaa aagttgaggt
     3841 tgacgagtcg gaaacagacg atgccgatta tgatatcgat cctggcagtg aaccggaaaa
     3901 cggcgaaaca accgagtaaa ttggcaaaat aattgcttga tttaataata ttgataattt
     3961 tgttaatgat ttaacaacta ataaaatagc attacgtatg aaaaacttaa tcctgacagt
     4021 gaccgcaatg atgttagttg ctgtagtatt cggccagacc aacaaaagaa caagcgcctt
     4081 caattacaat aattacggaa agctcgatct tgccaaagag gcgattgatg aagcagcaca
     4141 gcatgaaaaa acgatcatgg atgcaaagac atggttttac agggggaata tctattataa
     4201 tatcggtgtg agtctcgatt ccaattaccg gaaactggac ccggatcctt tcggtgtatc
     4261 tctcaaatct tatctgaaag ctaaagaact ggatgtaaaa ggcgagttca aggaaaatat
     4321 cgatcagaat atcgtggtga tcagccaggg atatttcaac atgggtgtat tttactataa
     4381 tgaacagaat tatccgttgg cagcaaaaaa ctttgagcag gcctacgagg taagtaagat
     4441 tgctaaccaa ctggatacca ctgccttatt taatgctgcc atttcggcga gagatgctca
     4501 cgacagggcc acggcaaaaa aatattacct ggagatcatc aaaatgaatg tttcgaatcc
     4561 ggtggtttat gatagtctag ccaatattta taaagcggag ggggacacgg ctatggcact
     4621 gcagaccatt caacaggggc gtcaggtatt taaagaggac ctgaatcttg ttatagccga
     4681 gaccaatatt tatcttcaga caaatcagca ggataaagcg ctggccaacc ttgaaacggc
     4741 agccaagctc gatccgacaa atccgacggt gttctgggcc agtggtacca tatacgacca
     4801 actgggagag caggacaagg ccataaaggc ctatgaaacg gctatccagc taaaacccga
     4861 ctatttcgta gcctatttta acctgggcat tctttatttc aaccaggcta cggaagtg
//
LOCUS       DAOQXS010000189         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17558_length_4900_cov_3.576264, whole genome shotgun sequence.
ACCESSION   DAOQXS010000189 DAOQXS010000000
VERSION     DAOQXS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17558_length_4900_cov_3.576264"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKM34_06380"
     CDS             complement(<1..228)
                     /locus_tag="PKM34_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83251.1"
                     /translation="MPLAIPYPLRGNYHEYPISSTIADPLRGSGETVGGEQSAVSGQR
                     SAVSSRQSAVSGQQSAVGSQRSAVSGQQSAVG"
     gene            378..638
                     /locus_tag="PKM34_06385"
     CDS             378..638
                     /locus_tag="PKM34_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83252.1"
                     /translation="MKRRFLIFLIISHIILIASPGWSQEFKMPSFVHAIDACDMDMDG
                     SVDIIVYARIPRDGVPRDGVPFLNSPGTAMTIQAMNARQECI"
     gene            806..2674
                     /locus_tag="PKM34_06390"
     CDS             806..2674
                     /locus_tag="PKM34_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNQ83253.1"
                     /translation="MMKKGIAFSVLTFLFLIVNPLFSQTPGYDAEYQKLVKEYILQKD
                     GSWDFHYRKEIKLLTHFSFHRLFGETFIVYNPLYQQLKINEAFTVMADGKKVIVPQNA
                     FNGVLPGFAANIPAYNHLREMVVTHTGTEVGSVITLDYTIHSAGNFLPFFFGMEEIGE
                     NVPVKDMLIIVRVPEEIILKERILNSRLAPEVIQLAGQKSYTWSFRDVPALPRSSNQD
                     IEQKTALFFSTAKDMTWAYFSFVNQEAFKSRPGAEISRRVDEVKKDKKSDLDIILALQ
                     AVVIDELRPADIPLANSGYKIRTPDEVWKSANATALEKAVLLANMLKLANINACPVAS
                     VTNAWYADDMGNPAIFDGFMVQVNPKETGRIYLPVTQKQAQNLMFDVSDKTLIQLDGA
                     IESMRTFREKAVENELEMKGELRLYDGKTVGGEVQMVMEGICHPFFKLRKDSSYVKNM
                     ITGDLPLSSVRNIKLRKISELSSEYDLTWNPELVPAFVGMTRNPEPGTSNDTGFFYFD
                     LPRFKTGFDAWNLVQFTGSGDTPVRLDFPLEEDYSFEIILPESYSLFTPPVDIKIEND
                     LGELAIKISQSGSKVKIERSWELYHDVVSSDLMDEFKEMIIAWEKVEHRKIILKSR"
     gene            complement(2764..3699)
                     /locus_tag="PKM34_06395"
     CDS             complement(2764..3699)
                     /locus_tag="PKM34_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83254.1"
                     /translation="MHTPIKKLSYDELLEILRHYDTESIFEKVNLVPDSEDLHESLAE
                     YINLRSFAKRAVLGIDIYRYGMYKHLEQVLIPPLFKILFEKAIRLCLENNRFVFQHYT
                     KEKIEKSFISTGDGGFVIFDTPLHALFFAINFEMVIRAYNSYHLFPKLRNIIGSLSLR
                     YAISYDTIFTFDNNFYGSAIITNARILNKDTLNRCLIDQQTYDWFLISIDGLENLQVL
                     TIADIANIYDFLDYDRKWIAEGTNEILDTNAENRRTGIINSDVLKVGQIQAKETLVNI
                     FNLHLQISMQIFSDRKVESKRTITVSLGNLNTSGI"
     gene            complement(3898..4473)
                     /locus_tag="PKM34_06400"
     CDS             complement(3898..4473)
                     /locus_tag="PKM34_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ83255.1"
                     /translation="MDDHSFLSLVQKRQSVRKYTRKPVEDEKLRRCLEAARLAPSASN
                     SQPWKFIVVNDPELVMKVAKETIGPLSTFNNFVPEAPVILAVVIEKMKVFTKIGASIQ
                     DREYPLIDIGIAAEHFCLQAAEEGLGTCMLGWFNEKPIKELLKIPKERRIGLLITLGY
                     APDDYRLREKIRKKPEEMISFNFYKSHKNTL"
     gene            complement(4648..>4900)
                     /locus_tag="PKM34_06405"
     CDS             complement(4648..>4900)
                     /locus_tag="PKM34_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ83256.1"
                     /translation="AGKRIGYVNHMAEKGTVMAHVDGGVPNIRIVVPEISEYVMGQLI
                     YFFELACGLSGYLLGVNPFDQPGVEAYKKNMFELLGKPK"
BASE COUNT         1264 a         1165 c         1221 g         1250 t
ORIGIN      
        1 gccgactgcc gactgctgac cgctgactgc cgaccgctga ctgccgactg ccgactgctg
       61 accgctgact gccgactgcc gactgctgac cgctgaccgc tgaccgctga ccgccgactg
      121 ctcaccgccg accgtttccc cacttccgcg tagcggatcc gctattgtag atgaaattgg
      181 atattcgtga taatttcccc gtaggggata aggtattgct aaaggcatag agagtagtag
      241 tatggaaaat gcaaaatgcc aaatgaaatg caaaatgcaa aatgcaaaac gcaaaatgcg
      301 aacatcttag gggtcccttt ttttcatata agtctgaaca aaatatgatt aactttagcc
      361 attcaaagac tttaacgatg aaaagaagat tcctaatatt tttaatcata tcccacatca
      421 ttttgattgc cagcccgggt tggtcacagg agtttaagat gccttccttt gttcatgcca
      481 tcgatgcctg cgatatggat atggatggaa gcgttgatat cattgtttat gcgcgtatcc
      541 cccgggatgg cgtcccgagg gatggcgtcc cttttttgaa ctcacctgga acggccatga
      601 cgattcaggc aatgaatgcc aggcaggagt gtatatgatt gttgccgagg ggaatagttg
      661 tcgtattttt acgaaagtgg tgaaaacgtg agacgtgaag cgtgaagcgt gattggtgga
      721 aaatgcaaaa tgaaatgcaa aatgaaaaat gcaaaacgca aaatgcgaac atctttttta
      781 attttacccc aaattaactg ctgaaatgat gaaaaaaggt attgcatttt ctgtcctgac
      841 atttctgttt ctgatcgtaa acccgctttt ttcccaaacc ccgggatacg acgccgaata
      901 ccagaaactg gtgaaagagt atattcttca gaaagacggc tcctgggatt ttcattaccg
      961 caaggagatc aaactgctca cccatttttc attccaccgc ctcttcggcg agacgtttat
     1021 cgtttacaac ccgctatacc agcagttgaa gatcaatgaa gccttcaccg tgatggccga
     1081 cgggaaaaaa gtgatcgtgc cgcagaacgc cttcaacggg gtgctgcccg ggtttgccgc
     1141 caatatcccg gcatacaacc atttacgaga aatggtggtg acgcataccg gcacggaggt
     1201 cggcagtgtc ataacactcg actatacgat ccattccgcc gggaactttc tcccgttctt
     1261 cttcgggatg gaagagatcg gggagaatgt cccggtaaag gacatgctga tcatcgtgag
     1321 ggttcccgaa gagatcatcc tgaaagaaag gatactgaac agccgcctgg cccccgaggt
     1381 cattcagctc gcaggacaga aaagctatac atggtcgttc cgcgatgtgc cggcgcttcc
     1441 ccgcagcagt aaccaggata ttgaacaaaa aaccgccctg ttcttttcga ccgcaaagga
     1501 tatgacctgg gcctatttct cgtttgtcaa ccaggaggct tttaaaagca ggccgggcgc
     1561 cgaaatatcc aggcgtgtgg atgaagtgaa gaaggataaa aagagcgacc tcgacatcat
     1621 cctggcatta caggcagtag ttatcgacga actcaggccg gccgatattc cgctggccaa
     1681 tagcggctat aagatccgaa cccccgacga ggtgtggaaa agcgccaatg cgaccgccct
     1741 tgaaaaagcg gtcctcctgg ccaacatgct taaactggcc aatatcaatg cctgcccggt
     1801 ggcatcggtc acgaatgcct ggtatgccga cgatatggga aatcctgcca ttttcgacgg
     1861 tttcatggtg caggtcaatc cgaaggaaac cggcaggatc tatctcccgg taacgcagaa
     1921 acaggcacaa aacctgatgt tcgatgtcag cgataaaacc ctgatccagc tcgatggcgc
     1981 catcgaatcg atgcggacgt tcagggaaaa agcggtggag aatgaattgg agatgaaagg
     2041 ggagttgaga ctttatgacg gtaagacggt aggaggggag gtgcaaatgg ttatggaagg
     2101 gatttgccat ccgtttttca aattgagaaa agattcgtcg tatgtgaaaa acatgattac
     2161 cggggattta ccgctatcct ccgtcaggaa tatcaaattg agaaaaatct ccgaactctc
     2221 ttcggaatat gatctcacct ggaacccgga acttgttccc gctttcgtgg gaatgacgcg
     2281 aaacccggaa cccggaactt caaacgatac cgggttcttc tactttgacc ttccccgctt
     2341 caaaaccggc ttcgatgcct ggaacctggt gcagttcacc gggtcgggcg atacgcctgt
     2401 aaggcttgat tttccgctgg aagaggatta cagttttgag atcatcctgc ccgaaagcta
     2461 cagtttgttt acccctccgg tggatataaa gatcgagaac gatctgggcg aactggctat
     2521 taaaatcagc cagtcgggtt caaaagttaa aatagagcgt tcctgggagc tttatcatga
     2581 tgtcgtttca tccgatctta tggatgaatt caaagaaatg atcatcgcct gggaaaaagt
     2641 cgaacaccgt aaaatcattc tgaaaagccg ttaaaaaccg tgttaattcg tctaatttgt
     2701 gtaattcgtg ttctattgag atgatagcac acgaatgata cgaattttac gaatattcac
     2761 ggattatata ccggaggtat taagattacc caaactgacg gtgatcgtcc gcttgctctc
     2821 cactttccgg tcggaaaaga tctgcatcga tatctgcaaa tggaggttga aaatgttgac
     2881 cagtgtctcc ttcgcctgaa tctgcccgac tttcagaaca tcggaattga tgatgcctgt
     2941 acgacgattt tcggcattcg tgtcaaggat ctcattcgtc ccttctgcaa tccatttccg
     3001 gtcataatcc aggaaatcgt aaatattggc gatgtcagca atagtaagga cctgcaggtt
     3061 ctccagcccg tcgatgctga tcaggaacca gtcgtaggtc tgctggtcga tcaggcaccg
     3121 gttaagggtg tctttgttca ggatacgggc attggtaatg atggcgctgc cgtagaaatt
     3181 attatcgaag gtgaaaatgg tatcgtagct gatggcatag cgcaggctca ggctgccgat
     3241 gatgttccgc agcttcggga atagatgata ggaattgtaa gcccggatca ccatttcaaa
     3301 attgatggcg aagaacagag cgtgcagcgg ggtgtcgaaa atgacaaaac ctccgtcgcc
     3361 ggtgctgatg aacgattttt cgatcttctc cttcgtgtag tgctggaata caaaccggtt
     3421 attttccagg caaagcctga tggctttctc aaacaggatc ttgaacagcg gcgggatcag
     3481 gacctgctca aggtgcttgt acatcccgta ccggtatatg tcgataccga gaacggcccg
     3541 tttggcaaaa gagcgcaggt tgatatattc ggcgagcgac tcgtggaggt cttcgctgtc
     3601 gggcaccagg ttcacctttt cgaagatcga ttcggtgtcg taatggcgaa ggatctccaa
     3661 tagttcatca taggagagtt tcttgatcgg ggtgtgcatt gggagttaag attttttgca
     3721 aagatagaaa aagagttcca ggttccaggt ttcgcgtcat tcccgcgaaa gcgggaacag
     3781 gttccgggtt tcaggtttcg cgtctttccc gcgaaagcgg gaaccagttc cgggtttcgc
     3841 atagaagtcg atgagaaacc ttcacagtcc gacgactaaa gccgtcggtt caaaccttta
     3901 taaagtattt ttgtgggatt tgtagaagtt aaagctaatc atttcttcgg gttttttcct
     3961 gatcttttct ctcaaccggt aatcatccgg ggcatatccg agcgtgatca gcaatccgat
     4021 ccgcctctcc ttcgggattt tcagcagttc tttgatcggt ttttcgttga accatccgag
     4081 catgcaggtt ccgagccctt cttcggcggc ctgcaggcag aaatgctccg ccgcaatgcc
     4141 gatatcgatc agcgggtatt cacggtcctg gatcgaagcg cctattttgg tgaagacttt
     4201 cattttttcg atcacaactg ctaggatgac gggagcttcg ggcacgaaat tgttgaaggt
     4261 cgacagcggg ccgatggtct ctttggccac tttcatgacc agctcggggt cattcaccac
     4321 aatgaatttc cacggctggc tgttgctggc cgacggcgcc aggcgtgccg cttcgaggca
     4381 ccgccgcaat ttttcatcct ctaccggctt acgggtatat ttccgaacgc tttgacgttt
     4441 ttgtactaat gagagaaaac tatgatcatc catgatattt tgtaattgat tctcttcgtg
     4501 taacttcgtg ccttcttcgt gaatcttcgt gtactaaagc aaggttaaat tgtttcacga
     4561 agttccgcga agaagacacg aagaaccacg aagcattaat aattttcccg gactaaacac
     4621 ttaaccctca atccttaaca cataaaacta tttgggtttc ccaagcaact cgaacatatt
     4681 cttcttatac gcttccaccc ccggctgatc gaaaggattg acgccgagca ggtagccgct
     4741 cagcccgcag gccagctcga agaaatagat cagttgcccc atgacatatt cgctgatctc
     4801 gggtaccacg atccggatat tgggtacgcc tccgtcgacg tgtgccatca ccgtcccttt
     4861 ttcggccatg tggttcacat agccgattcg cttgccggcc
//
LOCUS       DAOQXS010000190         4880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17638_length_4880_cov_3.197513, whole genome shotgun sequence.
ACCESSION   DAOQXS010000190 DAOQXS010000000
VERSION     DAOQXS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17638_length_4880_cov_3.197513"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="coaBC"
                     /locus_tag="PKM34_06410"
     CDS             <1..990
                     /gene="coaBC"
                     /locus_tag="PKM34_06410"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNQ83257.1"
                     /translation="WNSHVELGRWADLYLLAPVSANTLAKMAGGIADNFFLTACLSAK
                     CPVFFAPAMDLDMYKHPATMKNIQILQSYGNQLIEPSVGELASGLCGAGRMEEPENIF
                     GLVKSYFENKTGPLSGKRVLVTAGPTHEAIDPVRYIGNHSSGLMGFSLAEQAAEAGAI
                     VTLISGPTDLKLAGSSIERINVTSAAEMHHECLAASKESDIIIMAAAVADFRPVSTSS
                     QKIKKSGNSFLLELIPTKDILTEISANRKPGQVIAGFALETENEIDHATEKLVKKNLD
                     LIILNSMREEGSGFKTPTNKVSILFKSGQIKHYPLKSKKEVACDIIEAISSMI"
     gene            1031..1927
                     /locus_tag="PKM34_06415"
     CDS             1031..1927
                     /locus_tag="PKM34_06415"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNQ83258.1"
                     /translation="MKRIIIMLFLLASVIQSYSQEFYCNVDVTSKQIQSSDKRIYESM
                     RNAIYEFMNNRQWTNYNYKFNEKLECSILINVVERASTELFRCEMTIASRRPVFNSNY
                     NSPLFNFIDKNVDFEYIENQPLDFNTGTFSSNLTSILAYYAYIMLGLDFDTFQLNGGN
                     PYYEAALSIVNAASTSSYTGWNSAEGNKNRFWLVENLTNPAYTGIRSFYYDYHMKGLD
                     IMYEKPEEGRKAILQSLGYLQQVKQSRPGLFFLQIISDAKRDEFVNVFSEGLPTEKTN
                     AVKILNELDPSNAMTYQKIIQN"
     gene            1939..3591
                     /gene="recN"
                     /locus_tag="PKM34_06420"
     CDS             1939..3591
                     /gene="recN"
                     /locus_tag="PKM34_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNQ83259.1"
                     /translation="MLTHLSISNYTLIDRLDIEFHNGFSAITGETGAGKSILLDALDL
                     VLGKRAESQVLLDPSKKCVVEASFVIGKYNLGPFFLSNSLDYDEECILRREIHPNGKS
                     RAFINDTPVTLLQMKALGDQLVDIHAQHATTSLQDPLFQLSVLDGYANLNEQLHQFRE
                     RFMALRNLKDQKEMLMDEERKARTSMDYRQFLFDELNAAALQSDEQESLEERLKLVSN
                     AEEIKNTLYFIQGILNEDDNSILNQLTHAESQLNKIEGYYPPLKQIAERLRSNLIDIK
                     DINLEISKLDEDVEFSQELMNDLQARLDLIYKLQHKHQVTNNGSLIEIQNTLKQQLES
                     FTSLEEKINETGKQILAMESALDEAASLLTAARRKTFPELEKEILGYLRTMGMPDAQF
                     EIRWNLSPEMTVFGRDNIEFYFNANLGHSLKPLAEIASGGELSRVMLAIKTVLSGKKS
                     LPTIIFDEIDSGLSGDIAGKVGDVMRDAAKRIQIITVTHLPQIAGKARHHYSVFKSTA
                     RDVTFSNIKYLDDQERIAELAKLIGGRETTDASMAAAKELIN"
     gene            3614..4447
                     /locus_tag="PKM34_06425"
     CDS             3614..4447
                     /locus_tag="PKM34_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83260.1"
                     /translation="MAYNLLKGKKGIIFGALDEKSIAWQVAVKAFEEGAEFTLSNTPT
                     AIRFGTIDDLAKTCNTIVIPADATKVEDIENVFEKSMERFGGKVDFVLHSIGMSMNVR
                     KKRVYNEVDYDYFKTTLDISALSFHKIIQVAYRLDAIKEWGSVVALSYIAAQRTLYAY
                     NDMADAKALLESIARSFGYIYGREKKVRINTISQSPTMTTAGSGVRVMGGLMDFTERN
                     SPLGNATAEECANYCITLFSDLTRKVTMQNLYHDGGFSSMGMSLKAMTMYNKSFNCEH
                     C"
     gene            4538..>4880
                     /locus_tag="PKM34_06430"
     CDS             4538..>4880
                     /locus_tag="PKM34_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ83261.1"
                     /translation="MISFQLDTKQQEIIEKYRDFTKRWIIPNRMKYDELATFPWDIVK
                     AAYDEKIINASMPVEYGGHGHSIFEGAIASEELGYGCTGIGICIDASTLALTPLLLMG
                     SDEQKKRIFGEI"
BASE COUNT         1494 a         1066 c         1079 g         1241 t
ORIGIN      
        1 tggaacagcc atgtggagct ggggcgatgg gctgatctct acctgctggc cccggtatca
       61 gccaatacac tggcaaaaat ggccggtgga attgccgata acttttttct tacagcctgc
      121 ctttcggcga aatgccctgt tttctttgcc ccggctatgg acctggatat gtataaacat
      181 ccggcgacca tgaaaaatat ccagatcctt caatcctacg gcaatcaact tatcgaaccg
      241 tcggtcggag agctggccag cggattatgc ggcgccggcc gaatggaaga accggaaaat
      301 attttcggtc ttgtcaaatc ctattttgaa aataaaaccg gaccattatc agggaagaga
      361 gtcctggtta cagccggacc cactcatgaa gctatcgatc cggtgcggta tatcggaaat
      421 cactcctccg ggctgatggg attctcactg gcagaacagg cagccgaagc cggcgctatc
      481 gttacactca tctccggtcc gacagacctg aaactggccg gttcatccat cgaaaggatc
      541 aacgtgacca gcgccgccga gatgcaccac gaatgcctcg ccgccagcaa agaatccgac
      601 atcatcatca tggctgccgc tgtggcagat ttcagacctg tcagcacctc atcccaaaaa
      661 attaaaaagt cgggcaattc attcctgtta gaacttatcc cgacaaaaga tatcctgacc
      721 gaaatatctg caaacagaaa accggggcag gttattgccg gctttgcact ggaaacggaa
      781 aatgaaattg accatgcaac cgaaaaactg gtgaaaaaga atctggacct gatcatactg
      841 aattctatgc gcgaagaggg gagcggcttt aaaaccccca ccaacaaagt ctccattctc
      901 ttcaaatccg gtcagataaa acattatcca ttaaaaagta aaaaagaagt tgcctgtgat
      961 atcattgaag ctatttcttc aatgatatga ttttgttatt tttacactaa gtcaattaaa
     1021 taaagacttt atgaaaagaa tcataatcat gctctttttg cttgcttctg tcatacagtc
     1081 gtactcacaa gagttctatt gcaatgtcga tgtcactagc aaacaaatac agagttccga
     1141 taaaagaatt tatgaatcca tgcggaatgc tatttacgaa ttcatgaaca accgccaatg
     1201 gaccaattat aattataaat tcaatgagaa actggagtgt tccattctga tcaatgttgt
     1261 tgaaagagct tcgaccgaat tgttccgttg cgaaatgacc atcgcctcca ggcgacccgt
     1321 ttttaactcc aactacaatt ccccattgtt caactttatt gacaaaaacg ttgattttga
     1381 atatattgaa aaccaacccc tggattttaa caccgggaca ttttcatcca atctcacatc
     1441 gatacttgcc tactatgcct atatcatgct cgggcttgat tttgacacgt ttcagttaaa
     1501 tggcggaaat ccctattatg aagcggctct gagcatcgtc aatgcggcat ccacttcaag
     1561 ttataccggc tggaattcag cggaaggcaa taaaaacagg ttctggctgg tggaaaacct
     1621 gaccaaccct gcatatacag gtatcagaag tttttattac gattatcata tgaagggatt
     1681 ggacataatg tatgaaaaac cggaagaggg gagaaaagca atactgcaat cgcttggcta
     1741 ccttcagcag gtaaaacaat cacgccccgg tttgttcttc ctgcaaatta tctccgatgc
     1801 aaaaagggat gagtttgtca atgttttttc ggaaggatta ccaactgaaa aaacaaatgc
     1861 agtgaagatc ctcaatgaac ttgatccttc caatgccatg acgtaccaga agattatcca
     1921 gaattaaaag ccacgtaaat gctgacccat ctctcgatat caaattacac cttaattgac
     1981 cggcttgata ttgagttcca taatggtttt tcagcaatta caggtgagac aggtgccggc
     2041 aaatcgatcc tgctcgacgc acttgacctg gttctgggca aaagggccga atcccaggtc
     2101 cttttggatc cctccaaaaa atgtgtcgtg gaggcatctt tcgttattgg aaaatacaat
     2161 ctgggccctt tctttctttc caatagtctg gattatgatg aagaatgtat tcttcgccgt
     2221 gaaattcatc cgaacggaaa atcgagggcc tttatcaacg atactcccgt aaccctgctt
     2281 caaatgaaag cactgggcga tcaactggtg gatatccatg cgcagcatgc aaccacatcc
     2341 ttgcaggatc ctttgtttca attgtctgta ctggatggtt atgcaaacct gaatgagcaa
     2401 cttcatcaat ttcgggaacg attcatggca ctgagaaatc tcaaggatca aaaagaaatg
     2461 ctgatggatg aggagcgaaa ggccaggaca tcaatggatt acaggcagtt tttattcgat
     2521 gaactgaatg ctgctgctct gcagtccgat gaacaggaat cgctggaaga aagactgaaa
     2581 ctggtctcca atgctgaaga gataaaaaat acgctatact tcatacaagg aattcttaat
     2641 gaggatgata acagcatcct taaccagttg acccatgccg aatcccaact taataaaatc
     2701 gaaggatact atccaccgct gaaacaaata gcagaacggc tcaggtccaa tctgatcgac
     2761 attaaggata ttaatttaga gatcagtaaa ttagatgaag atgttgaatt cagccaggaa
     2821 ttaatgaatg acttacaagc ccggcttgat ctgatttata aattacaaca caaacaccag
     2881 gtcacaaata acgggtcttt gatcgaaatt cagaataccc tgaagcaaca actggagtca
     2941 ttcacctctc tggaggaaaa gataaatgaa accggaaaac agatcctggc tatggaatcg
     3001 gcccttgatg aagcagcatc tttattgacc gctgcccgcc ggaagacctt tcctgaactg
     3061 gaaaaagaga ttctggggta tttgcggacc atgggcatgc cggatgcaca atttgaaatc
     3121 cggtggaatc tgtcgcctga gatgacagtc ttcggccgcg ataatattga attctatttc
     3181 aatgccaacc tgggccattc attgaagccc ctggccgaga tagcgtcggg aggagaactt
     3241 tcgagggtaa tgctggcaat aaagacagtc ttatccggaa aaaaatcgtt gccgactata
     3301 attttcgatg agatcgacag cggcttgtcg ggtgatattg ccggcaaagt gggagatgtc
     3361 atgagggatg cggcaaaacg aatacagatc attaccgtaa cgcatcttcc gcaaattgca
     3421 ggcaaagcaa ggcaccatta ttcagtattt aaaagcacag cccgtgatgt cacattctcc
     3481 aatatcaaat atcttgacga tcaggaacga atagcagagc ttgcgaaact catcggaggc
     3541 agggagacaa ccgatgcctc gatggcagca gcaaaagaat tgatcaatta gtttaacttt
     3601 aaaatcaacg attatggcat ataacttact caaaggaaag aaaggaatca ttttcggtgc
     3661 actcgatgaa aaatcgattg cctggcaggt ggccgttaaa gcattcgagg aaggcgctga
     3721 gtttactctt tccaatactc ccacggccat tcgattcggt accattgacg acctggcgaa
     3781 aacctgcaat acgattgtta ttccagcgga tgcaacgaag gtagaagata ttgagaatgt
     3841 ttttgagaaa tcgatggaaa ggttcggcgg aaaggttgac ttcgtcttac attctatagg
     3901 tatgtcgatg aacgtcagga aaaagagggt gtataacgaa gtggactatg attatttcaa
     3961 gactactttg gatatttccg ctttgtcgtt tcataaaatt attcaggtgg cctaccggtt
     4021 agatgcaatc aaggaatggg gctccgtagt cgcattatcg tatattgccg cacaaaggac
     4081 actctatgct tataacgaca tggcagatgc caaagccctg ctggagtcta ttgcccggag
     4141 tttcggctac atttacggcc gggaaaagaa ggttcggatc aatacaattt cacaatctcc
     4201 aactatgacc acagccggaa gcggggtaag ggtaatgggc gggctgatgg attttacaga
     4261 aagaaactca ccgctgggca atgccactgc ggaagagtgc gccaattatt gcatcacctt
     4321 gttttctgac ctgacccgca aggtgactat gcagaacctg taccatgacg gtggtttttc
     4381 atcaatgggt atgagtctga aggcgatgac tatgtacaac aaaagcttta actgcgagca
     4441 ttgttaagat ttttcagctt aaaccagcgt catacaaata atattttata tttgttaagg
     4501 aattaaatac ctgatctcaa ccaaatccct taatactatg attagttttc agttagatac
     4561 caagcagcag gaaatcatcg agaagtacag agatttcaca aaacgttgga tcatacccaa
     4621 ccggatgaaa tacgacgaac tggctacatt cccctgggat attgttaaag ccgcatatga
     4681 cgaaaagatc ataaatgcct ccatgccggt tgaatatggc ggtcatggac atagcatttt
     4741 tgaaggagcc attgcatcgg aagagctcgg atacggctgt acaggcattg gcatctgcat
     4801 cgatgcgagt acactggcac ttacacccct tttgttgatg ggttccgatg aacagaaaaa
     4861 aagaattttc ggggagatca
//
LOCUS       DAOQXS010000191         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17731_length_4854_cov_4.013336, whole genome shotgun sequence.
ACCESSION   DAOQXS010000191 DAOQXS010000000
VERSION     DAOQXS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17731_length_4854_cov_4.013336"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..693)
                     /locus_tag="PKM34_06435"
     CDS             complement(154..693)
                     /locus_tag="PKM34_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83262.1"
                     /translation="MVLNQFGRIAQNEWYKLPDRFYNLQLGAFQVMPNHIHAIIVLHD
                     HTENDTAAGATLAVAPAAVGPIDNDPVAPVDVYPVAPIDVYPVDPDNNFATAGAGASP
                     APAVSPANPTVGNIIGAYKSLVANECLKIFKSKNEKMGKLWQRNYYERIIRNDRAFNA
                     ISNYILNNPAKWNAKKSIS"
     gene            1044..4040
                     /locus_tag="PKM34_06440"
     CDS             1044..4040
                     /locus_tag="PKM34_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNQ83263.1"
                     /translation="MNLLRKLVLPAIAALLIHSCPALSQILINEYSASNLSFIQDNYD
                     EYEDWIELYNAGPSGVNIGGYHLSDDTASPALWTFPAGVFLPSNGYLKIWASGRNEVS
                     GNHYHASFRLTQTKSSPDFVVLSDPDGVIIDQVQLAITQKDHSRGRAVSGGDQWAIFS
                     TPTPGSSNGNSTAYSAYAKKPVVSDTGGFYSNSVTITLMNPEPNSKIRYTLDGTEPYS
                     YATEYTAPISIFSTKILKARIFSNDPSILPGLIEFNTYFINANHSLPVLSIAGNELLE
                     LLNGNSALRPRGSIEFYNKLKQRTTTAYGEFNEHGQDSWVHPQRSIDYITRDEAGYNY
                     ALQQKFFSLSDRDEFQRVIIRAAGDDNYPGIDTSAHLRDDFVQTLSQKSGQHLDWRKS
                     ERVVIYANGQYWGIYALREKVHDHDYTEYYYDQGKYDIQFIMLWGGTWAEYGGQQALD
                     DWYVLHDFIMEHDMSNPFTYNAVAAQYDITSLVDYVILNSYVVCSDWMNWNVGWWRGL
                     DPEGSHRKWAYILWDEDATFGHYINYTGIPAQSPYVSPCFPEDLTDPWQDPEGHIAVL
                     NKLRTNDEFNQYYISRYIDLLNTTFTNDYMLNLLDSMAAIIAPEMPQHTQRWGGSVTQ
                     WQNNVQKIRNFITTRNSIMDAGLRNCWDLTGPYDLVIDAEPEGVGKVQINSLELNSFP
                     WDGDYFGGVDVKLSAIETSPYFEFDRWVLKNHEVTPSDTMKDVRLSLIMGDSVTALFK
                     PRVFEDSLVINEINYNSDGGFDPGDWVEFYNPHPYPLDISGWVFKDEDDAHEYEFPAG
                     TVIGQEGYLVLAGDTAAFDSLFPDVDNYLGPMGFGLSGNGELIRLYDNEGTLIDTVLY
                     DDEDPWPTEPDGDGATLELINPAWDNALAESWAASELYGTPGKENGNYVKVPDPVATP
                     DISCQVIPNPFRSNAIFRIRSEEILSNASIEVFDLYGRKVKEINGIQSNYIPLSGQGL
                     NAGIYIYRILDSKGMVLFQGKFVMTE"
     gene            complement(4094..4354)
                     /locus_tag="PKM34_06445"
     CDS             complement(4094..4354)
                     /locus_tag="PKM34_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNQ83264.1"
                     /translation="MKCPTCNVPLVMSERQGIEIDYCPQCRGVWLDRGELDKIIERSM
                     NEDQPKGFNQPPPQQRGHHDDYRHHGDYYKGRKKGWLGDLFD"
     gene            complement(4400..>4854)
                     /locus_tag="PKM34_06450"
     CDS             complement(4400..>4854)
                     /locus_tag="PKM34_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83265.1"
                     /translation="FMIIFFVLLGVYAIQIATKNGPLSIGYELLAAGFIFILVYFFRK
                     IILIRRIDYSAPVIIFLRKAEKRYTFITAADWFIIIPILGVIGTGGGYIVYYSFIKYF
                     EDSPWPLVIYIIFFVSVIIFGFWAGKKDWKKENGVILDKIKKSIDENR"
BASE COUNT         1272 a         1187 c         1121 g         1274 t
ORIGIN      
        1 tttgaagaat aagggaaaat ggatgcttcg aacggttatc atcgacgact attccatatt
       61 cgcgacaccc tcaccagtct gtttgaaagc aattgccccc aggtctcaaa gccggaatta
      121 gccgatcggg gaattgggaa tgataaaatc ggatcatgaa attgattttt tcgcattcca
      181 tttggccgga ttattcagga tatagttgga aatcgcattg aacgcccgat cgttccggat
      241 gatccgttcg taataattcc gttgccacaa tttacccatt ttttcgtttt tcgatttgaa
      301 tatttttaaa cattcgttgg caaccaacga tttatatgcc ccaattatat ttccgaccgt
      361 tggatttgcc ggtgatacag caggggcagg gctcgcccct gcccctgctg ttgcaaaatt
      421 attatccggg tcaaccgggt aaacgtcgat cggggcaacc ggataaacgt caaccggggc
      481 aaccggatca ttatcgatcg gtccaaccgc agcaggggca accgcgaggg ttgcccctgc
      541 tgcggtatcg ttttcggtgt ggtcgtgcaa aacaataatt gcgtggatat ggtttggcat
      601 gacctggaat gcacccaatt gcaaattgta gaaccggtct ggtaatttat accattcgtt
      661 ttgggcaatc ctgccaaatt ggtttaatac catcgtatgg ttttggatta ttccgaatcg
      721 aaatttatgg tcctgacaac aaatggtaat aaaatactta cctgcccggg aataatcata
      781 cccctttaat ctgagggacc tgcgatgatg tattgccgga ttgtatttga ccgccctgta
      841 catgatatcc tcccttctat cttccgccac actatcacct gcggcttaaa atgaaatgat
      901 tttgataagt tcttatcaaa aactttacca ctttaatgac ttttgcattt aaaatgaatt
      961 tccggaaatt attttatctg gaatttgact tattagtaat ttagcaggtc atcagaatct
     1021 gatcacccct taaacctgcc catatgaatc tccttcgaaa gttagtatta ccggctattg
     1081 cagcgctgtt gatccactca tgtcctgcat tatcccaaat cctcatcaac gaatactccg
     1141 cctccaacct gagctttatc caggataact acgatgagta tgaagactgg atcgagttgt
     1201 acaatgccgg cccttccggc gtcaatatcg gcgggtatca cctgagcgac gatacggcca
     1261 gccctgcctt atggaccttc ccggccggcg tattccttcc gtcgaacggt tacctgaaga
     1321 tctgggcgtc gggccggaac gaagtctccg gaaaccatta ccacgccagc ttccgcctca
     1381 cccagacaaa atcttcgccc gactttgtcg tcctctccga tccggatggc gtgatcatcg
     1441 accaggtgca gctcgctatt acacaaaaag atcattcccg cggacgtgcc gtcagcgggg
     1501 gcgatcagtg ggctatcttt tccaccccta caccgggctc ctccaatggc aactccacag
     1561 cctattcggc atacgccaaa aagcctgtgg tcagtgatac gggcggattc tatagcaatt
     1621 cggtcactat taccctcatg aatcccgaac ccaactccaa aatccggtat acgctcgacg
     1681 gaacggaacc atactcctat gctaccgaat acacggcccc catttcaatt ttttcaacca
     1741 agatactcaa agccaggatt ttcagtaacg atccctccat tcttcccggc ctgatcgaat
     1801 tcaacacata ttttatcaac gccaaccatt ctctgcccgt tctttccatt gcaggaaatg
     1861 aactgctgga gttgctcaac ggaaattcag cattgcggcc aaggggatcc atcgaatttt
     1921 acaacaagct gaaacagagg actaccacgg cttatggcga gtttaacgaa cacgggcagg
     1981 attcgtgggt ccatccacag aggagcatcg attatatcac ccgcgatgaa gccggttata
     2041 attatgccct tcagcagaaa ttcttctcct tgtcagaccg cgatgaattc cagcgggtga
     2101 tcattcgtgc cgccggcgac gacaattatc ccgggattga taccagcgcc catctccgcg
     2161 atgatttcgt ccagaccctt tctcaaaaat cgggtcagca tctcgactgg cggaaatcgg
     2221 agcgggttgt catttatgcc aacgggcagt actggggaat ctacgcctta cgcgaaaagg
     2281 tgcacgacca tgattatacg gaatattact acgaccaggg aaaatacgat atccagttta
     2341 tcatgctctg gggcgggacc tgggccgagt acggcggcca gcaggcactc gacgactggt
     2401 atgtcctgca cgacttcatc atggagcatg acatgagcaa cccgttcact tacaacgcag
     2461 tagctgcaca atacgacatc accagcctgg tggattatgt aatacttaac tcctatgtgg
     2521 tgtgttccga ctggatgaac tggaatgtag gctggtggcg cggattggat ccggaaggga
     2581 gtcaccggaa atgggcgtat atcctctggg atgaagatgc caccttcggg cattacatca
     2641 attataccgg tattccggcc caatcaccgt atgtttcacc ctgttttccc gaagacctta
     2701 ccgatccgtg gcaggacccg gaggggcata ttgcagtgct gaacaaactg cgcaccaacg
     2761 acgagtttaa ccagtactat atttcccggt acatcgacct tctcaacacc acatttacca
     2821 atgattacat gctgaacctg ctcgacagta tggcggcgat catcgcccct gaaatgcccc
     2881 agcatacgca gcggtgggga ggctcggtta cccaatggca gaataacgta cagaagatca
     2941 ggaatttcat cactacccgg aacagtatta tggacgccgg cctgagaaac tgctgggacc
     3001 tgaccgggcc ttatgatttg gtcattgatg ccgagccgga aggtgtcggc aaagtgcaga
     3061 tcaactcgct ggagttgaat tctttcccat gggacggcga ctattttggc ggtgtcgatg
     3121 taaaattgtc ggcaatcgaa accagcccct attttgaatt cgaccggtgg gtcttaaaga
     3181 atcatgaagt aacaccttcg gataccatga aggatgtcag gctgagcctg attatgggcg
     3241 attcggtgac tgctttgttc aagcccaggg tattcgagga ttccctggtg atcaacgaaa
     3301 ttaattataa ctctgacggg ggatttgatc cgggcgactg ggtggagttt tataatcctc
     3361 atccttatcc gcttgatatt tccgggtggg tttttaaaga tgaagatgat gcgcatgaat
     3421 atgaattccc agccggaacc gttatcggtc aggagggata tctggttttg gcgggcgata
     3481 cggcggcatt cgacagttta tttccagatg ttgacaatta cctggggcct atgggttttg
     3541 gcctttccgg aaatggcgag ctgatacggt tatatgacaa tgaagggacg ctgatcgata
     3601 ccgtgcttta cgatgatgag gatccctggc ccacagaacc tgatggcgac ggggcgactc
     3661 ttgaactgat caacccggcc tgggacaatg cgcttgccga aagctgggca gcttccgaac
     3721 tttacgggac acccgggaaa gagaatggta actatgttaa agtccccgat cctgttgcaa
     3781 cacctgatat cagttgccag gtgatcccga atccattcag gtcaaatgct attttccgga
     3841 tccggtcgga agaaattctg agcaacgcat ccattgaggt ttttgacttg tatggacgca
     3901 aagtgaaaga gataaacggc attcaatcca attatattcc tctgtcaggc cagggcctga
     3961 acgcaggaat atacatctac aggatattgg atagcaaagg gatggtgctg ttccagggga
     4021 aatttgtaat gacggaatag ggtaaaaaga tgagactcca tctcaaatct tgagatggag
     4081 tctcactgat ccatcagtca aaaagatcac ccagccagcc tttctttctt cctttatagt
     4141 aatcgccatg atggcggtaa tcatcatgat gcccgcgctg ttgtggcgga ggttgattga
     4201 atcctttcgg ctgatcttcg ttcattgacc gctcgatgat cttgtctagc tctccacggt
     4261 caagccatac gccacggcat tgcggacaat aatctatttc gatgccctgg cgttcggaca
     4321 tcaccaacgg aacattgcaa gtcggacatt tcatagctaa tattttttgc taatttaaac
     4381 atttatcctt cctgaaagtt taacggtttt catctattga cttctttatt ttgtcaagaa
     4441 tgacaccgtt ctcttttttc cagtccttct tcccggccca gaatccgaag atgatcaccg
     4501 acacaaagaa tataatgtaa atgacaagcg gccagggact gtcttcgaaa tatttgataa
     4561 agctataata aacgatgtaa ccacccccgg ttccaattac acctaagatt ggaatgataa
     4621 tgaaccaatc tgctgcagtg ataaaagtgt acctcttttc tgcttttctt aaaaatataa
     4681 tgaccggggc tgaataatca attctgcgga taaggatgat ttttcggaag aaatagacca
     4741 gaataaaaat aaaaccggct gccagcaatt cataaccgat ggagagcggg ccgtttttag
     4801 tggcaatctg tattgcatat actccgagta aaacaaaaaa aatgatcatg aatc
//
LOCUS       DAOQXS010000192         4836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17831_length_4836_cov_3.683121, whole genome shotgun sequence.
ACCESSION   DAOQXS010000192 DAOQXS010000000
VERSION     DAOQXS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17831_length_4836_cov_3.683121"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKM34_06455"
     CDS             complement(<1..488)
                     /locus_tag="PKM34_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83266.1"
                     /translation="MKTRAFLLVMLVMGSFALSFGQEQPVQDMKTLFKPTGKKISHGG
                     YIGVSVGYTQMKNENVITLGGRAGWLIDHHVTIGVAGNSFISNPYIKGNFPNTDDTAF
                     YLMGGYGGLLIEPIIAPNFPVHVSFPILIGGGGFILDDQSIFHRDSYEYGDGDYNYDY
                     YG"
     gene            complement(566..1093)
                     /locus_tag="PKM34_06460"
     CDS             complement(566..1093)
                     /locus_tag="PKM34_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83267.1"
                     /translation="MKISAKLLIVTLFMLSVIQAGAQENRKQAGFRFGNTTGFSARVI
                     TEDDFAFEGILGFRNGGLQLYGLFESRKPLYFNRIENMHLYFGGGVHVGFVRWNDYDK
                     YYDPYGNDHYYDDYYKWHTGMAFGLDGIVGMEYVFQAAPISLAVDFKPFFEMYGPFLM
                     RVNFWDFGFHMRYNF"
     gene            complement(1118..1894)
                     /locus_tag="PKM34_06465"
     CDS             complement(1118..1894)
                     /locus_tag="PKM34_06465"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNQ83268.1"
                     /translation="MKTKKFSVLLLLFAAGLFFTGCYKPWHVIEGNHDVQTDTRAISG
                     FSHVFNEGNFDVYIIQDASGEVIIEAESNLIPLIRTRIEGSALVIDTRDNLHNNYPMK
                     VYVHTPEIEKIRLSGSGLMHAENINTGDIDIDLSGSGDIFFSGIADDLECKISGSGDM
                     EIGIECNTIDAKISGSGEMEFYGSAHRGDLNISGSGSVRAYDLTLQDCYARISGSGDM
                     YLTVEDYLNVTISGSGDVYYLGNPIIETNISGSGNVIHPR"
     gene            complement(1965..3068)
                     /locus_tag="PKM34_06470"
     CDS             complement(1965..3068)
                     /locus_tag="PKM34_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83269.1"
                     /translation="MTLLSNYKYLLVIACMAFSFPLAAQVYKNNRTVREAFRIAPNTE
                     VQVINKYGDIHLIPWEKDSVVFQIELSVTSNKQSKVDKIYDYIDFDFKSTAYYVIAQT
                     VFEGQNTIWSEMADVASTIFSSGTNTRIDYTVYFPAGNDVRIENKFGNIYTTDHTGKV
                     DFTLSNGDLKSHSFSGPTRLKIEFGNATIDKIKNATIVLGYAELNLDMAEDLTFDSRS
                     SKVYINNCKKVHLDSKRDRFQVKSAGEITGETFFSYLNLDYVNSRINLKTSYGDLKLL
                     QVAPAFVRMDIHSQYSDVTVYYDEERLYEFDITRDDRSQVIAPATVLSKKEDPVAGME
                     KTFRAVITSGNINKSKVPIIINIKSGKIYLVES"
     gene            complement(3046..3618)
                     /locus_tag="PKM34_06475"
     CDS             complement(3046..3618)
                     /locus_tag="PKM34_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83270.1"
                     /translation="MSPDKLEKYMLEHRQEFDDMEPDPSLWERIDTRKAPVIRPNWMS
                     IGWKVAAGVAIFIASYFFHDYMSSRNNNQESFMAGLNEEASPIARELIEAEAYYTARI
                     SYTKDEVFRLAAADPDLRREVDLQMVDLDRVFTELKEDLKDNAANEEVIEAMIQNYRL
                     KLDILEDMLRQLKESKEPQNDTSHDAAIEL"
     gene            complement(3608..4186)
                     /locus_tag="PKM34_06480"
     CDS             complement(3608..4186)
                     /locus_tag="PKM34_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ83271.1"
                     /translation="MYNPIRLSNVEQADISRDIIERSKRGNRQAQYQLYRQYAKAMFN
                     ICNRMMNDRADAEDMLQESFSEAFRRLDSFRNESTFGAWLKMIVINRCINEIKRKKAQ
                     LEFFDDMSPFEDEEDLTDDQSAGLSPERIKKAMEDLPKGSRMIFSLYLLEGYDHQEIS
                     EILNISESNSKTQYMRAKQRIKEILKNQPYES"
     gene            4426..>4836
                     /locus_tag="PKM34_06485"
     CDS             4426..>4836
                     /locus_tag="PKM34_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNQ83272.1"
                     /translation="MKLPFAESYKIKMVESLYRSTKSEREEWIREAKYNLFNLRSGHV
                     FIDLLTDSGTGAMSDLQWSAMMLGDESYAGASSYFKMKEAIQTVLGFDYFLPTHQGRA
                     AENVLFSVLVKNGDIIPGNAHFDTTKGHIEFRHAS"
BASE COUNT         1246 a         1138 c         1079 g         1373 t
ORIGIN      
        1 taaccataat aatcataatt gtaatcacca tcgccgtatt catatgagtc cctgtggaag
       61 atcgactggt cgtcaagaat aaatccaccc ccgccgatca ggatcgggaa ggagacatgg
      121 acggggaaat tcggggcaat aatgggctcg atcagcaggc caccgtaacc gcccatcagg
      181 taaaacgcag tatcgtccgt attcgggaaa ttgcccttaa tgtatggatt gctgatgaag
      241 gaattcccgg ctacgccgat ggtaacgtga tgatcgatca gccagccggc acgcccgcca
      301 agggtgatca cattttcatt tttcatttgt gtatagccca ccgaaacacc gatatatccc
      361 ccatgactga ttttcttacc ggtgggtttg aataacgttt tcatatcctg gaccggctgt
      421 tcctgtccga acgataaggc gaaagatccc atcaccaaca ttacgagtaa aaatgctctt
      481 gttttcatta ttttagtttg ttaaatttct gaaaaatatc ttaaatactt tctaattaag
      541 aattaagaac taaaaattta aaattttaaa aattatatct catatgaaac ccgaagtccc
      601 agaaattgac acgcatcagg aacggcccgt acatctcaaa gaaaggtttg aaatcgacgg
      661 ctaaactgat aggtgcagcc tggaagacat attccatacc cacgatcccg tcgagcccga
      721 aagccatacc ggtgtgccat ttgtaataat catcgtaata atgatcgttt ccgtaaggat
      781 catagtattt atcgtaatca ttccagcgga caaaacccac atggacacct cctccgaaat
      841 acaggtgcat attttcgatc ctgttaaaat aaagtggttt tcttgattcg aacaggccgt
      901 acagttgcag tcccccgttg cgaaatccca ggatgccctc aaaggcaaaa tcatcttccg
      961 tgataacccg ggctgagaat cccgtcgtat tgccaaaccg gaaaccggct tgcttcctgt
     1021 tctcctgcgc tccggcctgt atcaccgaaa gcataaagag ggtgacaata agtagctttg
     1081 ctgatatctt cataattatc aggattttat gaggttatta ccgcggatgg ataacgtttc
     1141 ctgacccgga aatattcgtt tcgataatag gattgcccag gtaatagaca tcaccgctgc
     1201 ccgaaatggt gacattcaga taatcttcaa cggtcaggta catatcgccg gaaccggaga
     1261 tccgtgcgta gcaatcctgt agagtcaggt cataggccct gaccgatccc gaaccggaga
     1321 tattcaggtc gccgcgatgg gctgatccgt aaaactccat ctcccccgaa ccgctgatct
     1381 tcgcatcaat agtattgcat tcgatcccga tttccatatc gcccgaaccc gatatcttac
     1441 attccaggtc atctgcaata cctgaaaaaa agatgtcacc cgaaccgctc agatcgatat
     1501 cgatatcgcc tgtattgatg ttttcggcat gcatcagtcc cgatccgctc aacctgatct
     1561 tctcgatctc gggcgtatgc acataaactt tcatggggta gttgttgtgc aggttgtctc
     1621 tcgtgtcgat gaccagggcc gacccttcga tccgggtccg gatcagcgga attaggttgg
     1681 attcggcttc gatgatcact tcgccactgg catcctggat aatatacaca tcaaaattcc
     1741 cttcgttaaa cacatggctg aaaccggaaa tggcccgtgt gtcggtctga acatcatggt
     1801 tcccttcaat aacatgccag ggcttgtaac atccggtgaa gaataaccct gccgcaaaca
     1861 gtaataacaa tacgctgaat ttctttgttt tcataattta ttcttatttt gattttttta
     1921 ttaaagacaa gataaaggac aataggttac ttttttttta aaaattatga ttccaccagg
     1981 taaatttttc ccgatttaat attgatgatg atgggaacct ttgatttatt aatgtttccg
     2041 gaagtgatca ccgccctgaa tgttttttcc attcctgcca caggatcttc ttttttgctg
     2101 agtacagtag ccggagcaat gacctgtgac cggtcgtcgc gcgttatatc gaattcatag
     2161 agcctttcct cgtcatagta aaccgtcaca tcggaatatt gcgaatggat atccatcctg
     2221 acaaatgccg gggcaacctg taaaagtttg agatcaccgt agctggtctt caggttgatc
     2281 cgggagttca cataatcgag attcaaatat gagaaaaaag tctccccggt tatttctccg
     2341 gctgatttga cctggaatcg gtcgcgcttg gaatcgaggt gcactttctt gcaattgttg
     2401 atataaacct ttgaagaccg gctgtcaaaa gtgagatctt ccgccatatc aaggttaagc
     2461 tcggcatagc caagaactat ggttgcgttt ttgatcttgt cgatggtagc attgccaaac
     2521 tcgatcttca gtcgtgtcgg gccggagaaa ctgtgactct tcaggtcgcc gttcgacagc
     2581 gtaaagtcaa ctttccccgt atgatcggtc gtgtagatat ttccgaattt attctcgatc
     2641 ctgacatcgt taccggcggg aaaatagacc gtgtaatcga tgcgcgtatt ggtaccgctg
     2701 ctgaaaatag tgctggccac gtcggccatc tcgctccaga tggtgttctg cccttcgaat
     2761 acagtctggg cgattacgta ataagcagtc gatttgaagt cgaaatcgat ataatcataa
     2821 attttatcga ctttcgattg cttgttcgac gtaaccgaca gctcaatttg aaagaccacc
     2881 gaatcctttt cccagggaat aagatggata tcaccgtatt tgttgatgac ctgcacctcg
     2941 gtattgggtg cgatccggaa ggcttccctc acggtgcggt tgttcttgta aacctgcgcg
     3001 gccagcggga agctgaaagc catacaggct attacaagaa ggtatttata attcgatagc
     3061 agcgtcatgg cttgtgtcgt tttgtggttc ttttgattct ttcagttgcc tgagcatatc
     3121 ttccagtatg tcgagtttaa gacggtaatt ctggatcatg gcttcgatga cttcttcatt
     3181 ggcggcattg tccttcagat cttctttcaa ttctgtaaat accctgtcga ggtcaaccat
     3241 ttgaagatct acctcccgcc gaaggtcagg atcggcggca gccagccgga acacttcgtc
     3301 tttggtgtaa ctgatccttg ccgtgtaata cgcctctgct tcaatcagtt cgcgggcgat
     3361 aggggaggcc tcttcgttaa gtccagccat gaagctttcc tggttgttgt ttctcgacga
     3421 catataatcg tggaagaaat aagaagcaat gaagatggcg acgccggcgg caactttcca
     3481 tccaatgctc atccagttgg gccggatgac cggggcctta cgggtgtcta tcctttccca
     3541 gagcgatggg tcaggctcca tgtcgtcgaa ttcctgacgg tgctcaagca tgtatttttc
     3601 cagtttatca ggactcatat ggctgatttt ttaatatttc tttaatccgt tgtttggcgc
     3661 gcatgtactg tgtcttggaa ttggattcgc tgatgttcag tatctccgag atctcctggt
     3721 ggtcatagcc ttcaagcagg taaagcgaaa atatcatccg gcttcctttg ggcagatctt
     3781 ccatcgcctt tttaatccgt tcgggcgata acccggccga ctggtcatcg gtcaggtcct
     3841 cctcatcctc aaacggcgac atgtcgtcaa agaactccaa ctgggccttt tttcgcttga
     3901 tctcattgat gcaccggttg atcacgatca tcttgagcca ggcgccgaaa gtggattcgt
     3961 tgcggaaact gtcgagccgc cggaatgctt ccgaaaacga ctcctgcagc atatcctcgg
     4021 catcagcccg gtcgttcatc atccggttgc aaatgttgaa catggctttg gcgtattgcc
     4081 ggtaaagctg gtactgcgcc tgcctgttcc ctcttttgct ccgctcgatg atgtctctgc
     4141 tgatgtcagc ctgctccacg ttcgatagcc ttattggatt atacactgaa aaagacaata
     4201 aaaaaattaa gaggttgcaa tttttttaaa agtaaaaaaa atatttattt ttgacggtca
     4261 ggttatttca ttaattaatg atttacaggc aattcatatc tgataaactt atctacctga
     4321 atataaaaaa cagggacgaa ttccggattc caggttccgg gttccatgtt taaagttatt
     4381 ttatcagtta attaagaatt aagaatttaa aattcaaaat tgtctatgaa gttgcctttc
     4441 gcagaatcat acaagataaa aatggtcgaa tccctttacc ggagtaccaa atcggaaagg
     4501 gaggagtgga tcagggaagc aaagtacaat ctcttcaatc ttcgtagcgg gcatgtgttt
     4561 attgacctgc tgaccgattc ggggaccggc gccatgagcg acctgcagtg gtcggccatg
     4621 atgctcggcg atgaaagcta tgccggcgcc tcttcctatt tcaaaatgaa agaagcgatc
     4681 cagaccgtcc tggggtttga ctattttctt ccgacccatc agggccgggc tgccgagaat
     4741 gtcctctttt cagtccttgt aaaaaacggg gatatcatcc cgggaaatgc ccatttcgac
     4801 acgaccaaag ggcatatcga attccgccat gcttca
//
LOCUS       DAOQXS010000193         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17845_length_4833_cov_4.065299, whole genome shotgun sequence.
ACCESSION   DAOQXS010000193 DAOQXS010000000
VERSION     DAOQXS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17845_length_4833_cov_4.065299"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="PKM34_06490"
     CDS             <1..1733
                     /locus_tag="PKM34_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83273.1"
                     /translation="ILNTNMLANSDFLTGAFPAEYGNATAGIFDIHLRSGNTGRREYW
                     GQLGFNGLEFGTEGPFKTGGASSYLASYRYSFTGLAERAGIRLEESADYQDMGLRFSF
                     PTKKAGIFRFTALGGISAIRLMDSEQERNEWIFDSHGEDLRTGSHLAAAGLSHLFFIN
                     EDARIQSYLSFTSSGIETTIDTFELGSPKPFRWAGERSRENKISASVHYKYKINSQNN
                     LNAGVYYDLFMMNYLDSQFIKQEYIYHTDAKENLGLLRAYGGYQHRFGPAFAASLGLH
                     LQHLSMNGSFSAEPRFGIRWDVTPLSAISGGFGLHSQMVPSMLYFTQSRLPDGTYIQT
                     NRDLDFMRSLHAVAGYDYLIREHLRLKAEVFYQELYKIPVKESIPQYSVINEGLDYFI
                     LRYDSLVSRGSGTNFGLDLTMERFLHRNYYFLFTASLYRSDYTGYDNVKRNTAFDNRY
                     VVNAVGGYELPFGKMKNQFLVLGLRATWTGGRPYLPFDTERTTATGQVEYDWQNAFGV
                     RFEEYIRSSLRIGYRRNVTHANIMLLLDLQYRSNYTNIDLYRIDVKTGEIVQGYRMGF
                     YPMATWRIQF"
     gene            1765..3276
                     /locus_tag="PKM34_06495"
     CDS             1765..3276
                     /locus_tag="PKM34_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ83274.1"
                     /translation="MKIAIIGAGISGLSAGCYLQMHGFDTEIYEKHSRAGGLCTTWKK
                     GDYSFDGCIHWLLGSGERNPLYSLWSELIEMDSVKFVHHDTRFHIELKDNTDKYGSKV
                     FHLYNNIDRLEKYLLDLAPEDRVPIRKFIRMMRRIQGWEVPPEIKTPPSLFTFRQKIG
                     MARHLPLLLFMLKNRNLTNYSFAGRLKNPFLKEAFQLLFDGEEVSLLVIAFPLAFSDL
                     KATGYPIGGSAKFAERISERYISLGGRINYLSGVKEITVKDDAATGLLLEDGREIRSD
                     LVVSAADWRFTVFEALKGRYVNQKLLDLRDGKKFRVYYSVINVSLGIGRPMGDVPQLT
                     RFPLEKPLISPDGTVFERLELHTYHYDPTLAPAGKTSASASFYTSNGEFWIDLYREDR
                     ERYDQVRKAFAAEVIDILDKKFGRIKEYLEVTDVATPATYHLFTGNWRGSVQGWLPGK
                     NIMAPSPVTMKLPGLKNFYYASHWSVPGGGLPTALKSARDMAFTIAAEYRKKR"
     gene            3390..>4833
                     /locus_tag="PKM34_06500"
     CDS             3390..>4833
                     /locus_tag="PKM34_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83275.1"
                     /translation="MRAFRCLVLIIAFFLAQKGFPQQTLWVTHWADSGPGSLREVISS
                     ASAGDIIRFVPNISSITLSSAEIHINKPIKIIGNEDIITIKRNLSESTPHFRIFNISY
                     TDYPLDTLILKNLNFKYGFSSDGDDENQDGKDGGAIIASNPNNIIKIINCQFTNNQCG
                     NGITNLHVTTKSKKRADSGRGGHGGAIYSDAMLIIENCSFSLNETGSGGAIYLNNINW
                     FIEAGIGGHGGAIACNSYLIINDCLFNNNHTKSGGLAKSSSRYTSMALGGSAGYGGAI
                     YCSSGVEIINTTFTGNSTGTGGTGIGDGWVMGVGGDGGHGGALFAIDSTACVTNCTFS
                     ENKAGDGGLEGGDGAAHGGSGGYGGCIYTKNAWIIIDNCSFLNNTAGAGTSGSAYHAG
                     LGGDGGSGGAICAIDSQIEILESVFTDHCAGNGGYGGSAVWSIAGKGGHAGAVYVENS
                     YGAIKSTIFQRNHTGKAGDSYVQHEKIDTNI"
BASE COUNT         1256 a         1126 c         1290 g         1161 t
ORIGIN      
        1 ccatcctgaa cacaaacatg ctggccaact ccgacttcct gacgggcgct ttcccggccg
       61 aatacggcaa tgccaccgcc ggcatcttcg acatccatct ccgcagcggg aataccggca
      121 ggcgggaata ctggggccag cttggtttca acgggctcga attcggtacc gaagggccgt
      181 tcaaaacagg cggcgcctcc tcctacctgg cctcctaccg gtattcgttc accgggctgg
      241 cagaaagggc aggcatccgc ctcgaggaga gcgccgatta ccaggacatg gggctgcggt
      301 tcagcttccc gaccaaaaaa gcgggtattt tccgttttac cgccctgggc gggatctcgg
      361 ccatccggct gatggactcg gagcaggaga ggaacgaatg gatattcgat tcacacggag
      421 aagacctcag gaccggttcc cacctggccg cggccggact cagccatctt ttttttatta
      481 acgaagacgc acgcattcaa agctatcttt ccttcacctc cagcggtatc gaaaccacaa
      541 tagacacttt cgaactgggc agcccgaagc ctttccgctg ggctggcgaa aggtcgcgcg
      601 aaaataaaat ctcggcatcc gttcattaca aatacaagat caattcgcaa aacaacctca
      661 atgccggtgt ctactacgat ctctttatga tgaattacct tgacagtcaa ttcattaaac
      721 aggaatacat ctaccatacc gacgcaaagg agaaccttgg gcttctaagg gcttacggtg
      781 gctaccagca ccgcttcggg ccggcttttg ccgcttcact gggcctgcat ctccagcatc
      841 tgtcgatgaa cggctccttc tccgccgaac cccgctttgg gatccggtgg gacgtcaccc
      901 cgctgagcgc tatcagcggc ggatttggcc tgcacagcca gatggtgccg tcgatgctct
      961 atttcacgca gagccggctg cccgacggaa cgtacatcca gaccaaccgc gatctcgatt
     1021 tcatgcggag cctgcatgcg gtggcgggtt acgattacct catccgggag cacctgcggc
     1081 tgaaagcgga ggttttttac caggagctgt acaaaattcc cgtcaaggag agcatcccgc
     1141 aatactcggt gattaacgaa gggctggatt attttatcct gcgttacgac agcctggtga
     1201 gccggggcag cggcaccaac tttggcctcg atcttacgat ggaacggttc ctccaccgca
     1261 attactattt cctcttcacc gcatcacttt accggtcgga ttacacggga tatgacaacg
     1321 tcaagcgcaa tacggctttc gataaccgct atgttgtgaa tgccgtaggc gggtacgagc
     1381 tccctttcgg gaagatgaag aaccagttcc tggtcctggg cctccgggcc acgtggaccg
     1441 gcggacggcc ttacctgccg ttcgataccg aaaggaccac ggcgaccggg caggtagaat
     1501 acgactggca gaacgcgttc ggtgtccgtt ttgaggaata catccggagc agcctgcgga
     1561 tcggataccg gagaaatgtc acccatgcca acatcatgct gctgctcgac ctgcagtacc
     1621 ggagcaatta caccaacatc gacctgtacc ggatcgatgt gaagaccggc gagatcgtgc
     1681 agggttacag gatgggcttc tacccgatgg ccacgtggag gatccagttt tagcgggtgc
     1741 gggagttttt aatacctttg gaagatgaag atcgcgataa tcggagcagg catttcaggt
     1801 ttgtcggccg ggtgttacct gcagatgcac ggatttgaca cggagatcta cgagaagcat
     1861 tcccgtgcgg gaggattatg cacgacctgg aaaaaagggg attacagttt cgacgggtgc
     1921 atccactggc tgctgggctc cggtgagcga aacccgctgt actctctctg gtcggagttg
     1981 atcgaaatgg attctgtcaa atttgtccac cacgacacca ggttccacat cgagctgaag
     2041 gacaataccg acaaatacgg aagcaaggtt ttccatctct ataacaacat tgatcgtctt
     2101 gaaaaatacc tgcttgacct tgctcctgaa gacagggttc cgatcaggaa attcatccgg
     2161 atgatgcggc ggatccaggg ctgggaagtg cctcctgaaa tcaaaacccc gccttctcta
     2221 ttcactttca ggcaaaagat cgggatggca aggcacctgc ccctgttgct tttcatgctg
     2281 aaaaaccgga acctcaccaa ttactccttt gccggaagat tgaagaaccc gttcctgaaa
     2341 gaagcattcc aattgctgtt cgacggcgaa gaggtttcat tgctggtcat cgctttcccg
     2401 ctggcctttt ccgacctgaa ggccaccggt tatcccatcg gtgggtcggc gaaatttgcg
     2461 gagaggattt ccgaaagata tatttcattg ggtgggagga tcaattacct ttcaggggtt
     2521 aaggagatta cggtgaagga tgatgctgct acgggcctgc tgctggagga tggccgggaa
     2581 atcaggtcag acctggtggt gtcggcggcc gactggcgtt ttacggtgtt tgaggcgttg
     2641 aaaggcagat acgttaatca gaaactgctc gatttgcgtg acggtaaaaa gttcagggtt
     2701 tattattcgg tgatcaatgt ttcgctgggg atcggccggc cgatgggcga tgtaccgcag
     2761 ctgaccaggt ttccgctgga gaagccgttg atttcgcccg acgggaccgt tttcgagcgg
     2821 ctggagctgc atacctatca ttacgatccg acgctggcgc ctgccggcaa gaccagcgcc
     2881 tcagccagtt tttatacttc caacggcgaa ttctggatcg atctgtaccg ggaagaccgg
     2941 gagcgatatg atcaggtacg gaaagcgttt gccgcagaag tcattgacat cctggacaaa
     3001 aaatttggcc ggataaaaga atatcttgag gtgaccgatg tggctacgcc ggcaacttac
     3061 caccttttca cgggcaactg gagagggagt gtccagggat ggcttccggg gaagaacatc
     3121 atggctccat cgccggtcac catgaagctg ccgggtttga agaattttta ctacgccagc
     3181 cactggtcgg tgccgggcgg cgggctgccc accgcgctga agtcggccag ggatatggct
     3241 tttacgattg cagcggagta caggaagaaa agataaaggg aacattgtga atctataaaa
     3301 aaatttttat atttggaaag ccaataacca tcacaaaacc aataagcact tgaaatgacg
     3361 tcgaccgggt ttgtttatac caaactgata tgagagcatt cagatgttta gtattgatta
     3421 ttgcattttt tctggcacaa aaaggttttc ctcaacaaac attatgggta acccattggg
     3481 ctgactcagg gccaggttca ctgcgagaag ttatctcaag tgcctcagcc ggagatatta
     3541 ttcgatttgt tccaaacatt agttcaatta cactgagctc ggctgaaatt catataaata
     3601 aacccataaa aataattggt aatgaagata ttattacaat taaaaggaat ctttccgagt
     3661 ccactccaca tttcagaatt tttaacatct catatactga ttatccattg gatactttaa
     3721 ttttgaaaaa cctaaatttc aaatacggat tcagttcaga cggagatgat gaaaatcagg
     3781 atggaaagga tggaggcgcc ataatagcta gcaatcctaa taatattata aagataatca
     3841 actgtcaatt tactaataat cagtgcggta acgggattac taatttgcat gttaccacaa
     3901 aatcgaagaa aagagctgat tcaggcaggg gagggcatgg tggagccatt tactctgatg
     3961 caatgctaat cattgaaaat tgcagttttt ctttgaatga aaccggttca ggcggagcta
     4021 tctatttgaa taatattaat tggttcattg aagcaggtat aggggggcat gggggtgcaa
     4081 tagcttgcaa cagttacctc attatcaatg actgtctgtt taataataac cataccaaat
     4141 ctggcggcct tgcaaaatca tcttcgaggt atacttcaat ggcacttgga ggttcagcag
     4201 gatatggagg agcaatttat tgctcctcag gagtagaaat aattaatact acatttacgg
     4261 gtaattcaac cgggactggt ggcacaggta tcggtgatgg atgggttatg ggagttggcg
     4321 gggacggggg gcatggagga gctttatttg caattgacag caccgcatgt gtgaccaatt
     4381 gcactttctc tgaaaataag gcaggagatg gcggtcttga aggcggggat ggagcagctc
     4441 atggagggtc aggaggatat ggaggatgta tttatacgaa aaatgcatgg atcattattg
     4501 acaattgttc gtttctgaat aataccgccg gcgcaggaac cagtgggagt gcatatcatg
     4561 cggggcttgg aggtgatggt ggaagtggag gagcaatatg cgctatagat tctcaaattg
     4621 agatccttga gtctgtcttt acagatcatt gtgcggggaa cggtggatat ggaggaagtg
     4681 cagtatggtc gatagccggg aaaggaggcc atgctggagc cgtatatgtc gaaaattctt
     4741 atggagctat aaaatcaaca atatttcaac gaaatcatac aggaaaagca ggagattcat
     4801 atgtccaaca tgaaaaaata gatacaaata ttt
//
LOCUS       DAOQXS010000194         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17889_length_4826_cov_4.145672, whole genome shotgun sequence.
ACCESSION   DAOQXS010000194 DAOQXS010000000
VERSION     DAOQXS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17889_length_4826_cov_4.145672"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKM34_06505"
     CDS             <1..655
                     /locus_tag="PKM34_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ83276.1"
                     /translation="MISGLVRNYPDTLNPAIEVIGKKPGARLVSGMYIINSKKGSFFF
                     ADCSVNKNPTMEDLIEITLQTVYAVEQFKIKPRVAFVSYSNFGSNPGRVPDMQRDAVA
                     YLHKHYPNLVVDGEMQVNVALDPEIQKEHFPFSKLADGPANVFIFPYLTAGNIAYKLL
                     QVIADAEVIGPVINGMNKSVHVLQIGSTVTEIVNMVCVSAIDAMCVKARMAGEERCR"
     gene            823..1764
                     /gene="mdh"
                     /locus_tag="PKM34_06510"
     CDS             823..1764
                     /gene="mdh"
                     /locus_tag="PKM34_06510"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNQ83277.1"
                     /translation="MNKITVIGAGNVGATVANVLAQKDLAREVVLLDIKEGVAEGKAL
                     DIWQTSSILNFNTRVRGCTNDYLKTKGSGVIVITSGIPRKPGMSRDDLIKTNATIVKE
                     VVERSIRYSPDAIILIVSNPLDVMTYVAYKAANKESRKVFGMAGILDTGRYKAFIAEA
                     LDISPKDIHALLMGSHGDTMVPLRRYTSVGGIPVTELMNKEELDKIIERTRKGGGELV
                     DLMGTSAWYAPGAAVAEMVEAIVDDQKRVFPVCAYLNGEYGQKGIYLGVPVKLGKNGI
                     EEVIQLQLNKNEMKMVEDSANSVKEVMAVLDDMKLFD"
     gene            complement(1761..3317)
                     /locus_tag="PKM34_06515"
     CDS             complement(1761..3317)
                     /locus_tag="PKM34_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNQ83278.1"
                     /translation="MDRTESFKQKINAGYACQGGAIIMGSAMLDGEGVPGALVKIPLK
                     TMNRHGLIAGATGTGKTKTLQIIAEQLSENGVPVLLMDIKGDLSGIARPGTINQHIEK
                     RHSIMGEPWEGKGSPVEFLSLSDEKGARMRATVIEFGPVLLSRILDLNDIQAGVVSLA
                     FKYCDDNGIPLLDLKDFKKVLQYLSNEGKDEIKEEYGAVSSTSVSTIMRKIIEIEQQD
                     ADRFFGEESFNVDDLIRIDRNGKGIVSILRLTDIQDRPKLFSTFMLSLLAEVYNSFPE
                     VGDAEKPKLVLFIDEAHLIFNEASKALLSQIETIARLIRSKGVGIYFCTQNPTDVPDV
                     ILGQLGLKIQHALRAFTPKDRQMIMKTAENYPESEFYNVPDLLTSLGMGEALITALDE
                     KGRPTPLAATLLRSPMSRMDVLTNEEIDEIIDNSKLVPFYNREIDRESAFEILTARIE
                     SRQSSDVSRQSGEAKGRVGEEKGRKGEKEAPSVFEEISKNPMARQIGRTLVSELARGI
                     FGMLGGRRRR"
     gene            3431..4126
                     /locus_tag="PKM34_06520"
     CDS             3431..4126
                     /locus_tag="PKM34_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNQ83279.1"
                     /translation="MKKIAVLTGAGVSAESGLKTFRDHDGLWHDHRIEDVATPEAWER
                     DMDLVNQFYNERRKNLLEAMPNEAHIALARLEEKYNVTVITQNVDDLHERGGSSNVLH
                     LHGELKKVRSTVDPELVYELNGWELKRGDLCEKGSQLRPHIVWFGEAVPLIEDAAGIV
                     QEADIVIIIGTSLQVYPAAGLIHYAFAGAPKYYIDPKAVQVPFIPMLEIIRKPAGEGV
                     PELVDKLMLNVEF"
     gene            4132..4593
                     /locus_tag="PKM34_06525"
     CDS             4132..4593
                     /locus_tag="PKM34_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNQ83280.1"
                     /translation="MPEEQDLILAKLRSFCAYQERCIHDVTEKLEEWRIGQKRAEKII
                     EKLIKEDYLNEERFARTYAGGKFRINHWGKTKIIYELEKRQVPDLIIQIGLQEIDDQE
                     YKETLKEILTRKAREIKEKDPYRRRQKLTAYAVQKGYEYHLIREVAEQMNR"
BASE COUNT         1277 a         1181 c         1215 g         1153 t
ORIGIN      
        1 catgatatcg gggctggtaa gaaactatcc cgacactctc aatccggcca tcgaagtcat
       61 cgggaaaaag ccgggggcgc ggctggtatc gggcatgtat atcatcaaca gcaagaaagg
      121 atcctttttc tttgccgact gttcggtgaa caagaacccg acgatggaag acctgatcga
      181 gatcacgctg cagacggttt atgcagtaga gcagttcaag atcaagccca gggtagcttt
      241 tgtatcctat tccaatttcg gctccaaccc gggcagggtc cccgacatgc agcgcgatgc
      301 agtcgcttat cttcacaagc attatcccaa cctggtagta gatggtgaga tgcaggtgaa
      361 cgtagccctg gatccggaga tccagaagga gcacttccca ttctccaagc tggccgacgg
      421 cccggcaaac gtgttcatct tcccctatct tacggcagga aatatagcct acaagctgct
      481 gcaggtcatt gccgacgccg aagtgatcgg cccggtgatc aacggcatga acaagtcggt
      541 ccatgtcctg cagatcggca gcaccgtcac cgagatcgtg aacatggtgt gcgtcagcgc
      601 gatcgatgcg atgtgtgtca aagccaggat ggcgggagaa gagagatgca ggtgagatca
      661 aaattcaaag ttcaaagctc aaggttttaa gttttagatt ctttgttccg gattcaaagt
      721 tgggattgcg atattaatta tatcacaaat atttctattt ttgcatactt gaatctttcc
      781 aggttgaaca cgaatcgtaa aaaaaataaa aaaaacatcc ttatgaataa gattaccgtc
      841 atcggcgccg gaaatgtcgg tgctaccgta gcaaatgtcc tggctcagaa agatctggcc
      901 agagaagtcg tattactgga tatcaaagaa ggggtagcgg aaggcaaagc gctcgacatc
      961 tggcagacgt catccatcct gaattttaac acccgtgtca ggggatgtac aaatgattac
     1021 cttaagacca aaggctcggg cgtgatcgtg ataacatcgg gcattccgcg aaaaccgggt
     1081 atgtcgcgcg atgatttgat caagaccaac gcaactatcg ttaaggaagt cgtagaaagg
     1141 tccatccggt attctcctga tgcgatcatc ctgatcgttt ccaatcctct ggatgtgatg
     1201 acctatgtgg cctacaaagc ggccaacaag gaatccagga aagtcttcgg aatggcgggt
     1261 attctcgata cgggacgtta caaggctttt atcgccgaag cgctcgacat ttccccgaaa
     1321 gatatccatg ctttgctgat gggaagccat ggcgacacga tggtgccgtt aaggagatac
     1381 acttcggtcg gaggcatccc cgttaccgag ctgatgaaca aagaagaact ggataagatc
     1441 atcgagcgga cccgcaaagg cggcggcgaa ctggtcgacc tgatgggcac ctccgcctgg
     1501 tatgctccgg gcgctgcagt agccgaaatg gtcgaagcca tcgtcgacga ccagaaaagg
     1561 gttttcccgg tttgcgccta cctcaacggc gaatacggcc agaaaggcat ttacctcgga
     1621 gtgccggtga aactgggcaa aaacgggatc gaggaagtga ttcagctgca attgaacaag
     1681 aatgagatga agatggtgga agattccgcc aattcggtga aagaggtgat ggctgttttg
     1741 gatgatatga aattgtttga ttaacgccgc cggcgccctc ccagcatccc gaaaattccc
     1801 ctggccagtt ccgacaccaa cgtccggcca atttgccgcg ccatagggtt tttactgatc
     1861 tcttcgaaaa cagacggtgc ttccttctcc ccttttctcc ctttctcctc tcccactctt
     1921 ccctttgcct cgcccgactg acgactgaca tctgacgact ggcgactttc tatcctggcc
     1981 gtcagtattt caaaagcgct ctcccggtca atttcccgat tataaaacgg gaccagttta
     2041 gagttatcga tgatctcatc gatctcttca ttggtcaaaa catccatccg gctcatgggg
     2101 gaacgcaaaa gggtagccgc gagaggcgtt ggccggcctt tctcatccaa agcggtgatc
     2161 agcgcctcac ccatccccag cgaagtgagc aggtcgggca cattgtaaaa ctccgattcg
     2221 ggataattct cagcggtttt catgatcatc tgccggtctt tcggtgtgaa agcccgcaaa
     2281 gcatgctgaa tcttcaaccc gagctggccc aggatcacat ccggaacatc ggtggggttc
     2341 tgcgtacaga aataaatacc cacccctttc gaccggatca gcctggcgat cgtttctatc
     2401 tggctgagga gggctttgga agcttcgttg aagatcaggt gggcttcgtc gatgaacagc
     2461 accagcttcg gtttttccgc atcgccgacc tcggggaagg aattgtaaac ctccgcgaga
     2521 agcgaaagca tgaatgtcga aaacaatttc ggacggtcct ggatatcggt cagccttaaa
     2581 atcgacacga tcccttttcc attccggtcg attcttataa gatcatcgac attgaacgat
     2641 tcttccccga agaaccggtc ggcatcctgc tgctcaattt cgatgatctt tctcatgatg
     2701 gttgaaacag acgtcgatga tacagcgccg tattcttcct tgatttcgtc ttttccttca
     2761 ttggaaagat actgcagcac tttcttgaag tctttcagat ccagcagggg gatcccgttg
     2821 tcatcgcaat acttgaaagc cagcgaaacg acgccggcct ggatatcatt caggtcgagg
     2881 atacgggata acagaaccgg tccgaactca atcactgtcg ccctcatgcg ggcccctttc
     2941 tcatccgaca ggctgagaaa ctcgaccggg gatccttttc cttcccaggg ttcacccatg
     3001 atcgaatgcc gcttctcgat atgctggttg attgtccccg gcctggcaat gccgctcagg
     3061 tcgcctttaa tatccattaa caacaccggc accccgtttt ccgataactg ctcggctatg
     3121 atctgaagcg tcttggtctt cccggttccc gtagcgccgg caatgagccc gtggcggttc
     3181 atggttttca ggggaatctt caccagcgcc cccggtacgc cttctccgtc cagcattgca
     3241 ctgcccatga tgattgcacc accctggcag gcatagccag catttatttt ttgtttgaat
     3301 gattcggttc tgtccatcgg atgcatattt tcgacaaaaa taataatacg tgggtatgtt
     3361 agagacgcaa tattttgcgt ctctaacaaa tccacgaaaa aaattgtaat tttatagaaa
     3421 acaaaaccaa atgaaaaaaa ttgctgtcct gaccggcgcc ggcgtcagtg cggaaagcgg
     3481 gctgaagaca ttccgcgatc acgacggcct gtggcacgac caccggattg aagatgtggc
     3541 aacgccggaa gcctgggaac gggatatgga cctggtcaac cagttttata acgaaagaag
     3601 gaaaaatctt ctggaagcca tgcctaatga ggcacatatt gcgctggccc ggctggaaga
     3661 aaaatacaac gttacggtga tcacacagaa tgtggatgac cttcatgaaa ggggaggttc
     3721 atccaatgtt ttgcatttgc acggagaact gaagaaagtc aggagcacgg tcgatccgga
     3781 actggtttac gaattgaatg gatgggagct gaagcggggc gatttatgcg aaaaaggatc
     3841 ccagttgcgc cctcatattg tctggttcgg tgaagcggtt ccgctcattg aggatgcggc
     3901 gggaatagtg caggaggctg atattgtgat cattatcgga acgagtctac aggtttatcc
     3961 ggctgccggg ttgatccatt atgcatttgc cggggcacct aagtattaca tcgatcctaa
     4021 agccgtacag gtgccattca tccccatgct ggaaataatc cggaaacccg ctggcgaagg
     4081 ggtgccggaa ctcgttgata aacttatgct taatgttgaa ttttgaaggt tatgcctgaa
     4141 gaacaagatt taatcctcgc aaaactccgc agcttctgcg cataccagga gcgctgcatc
     4201 cacgatgtga ccgaaaagct cgaagaatgg cggatcgggc aaaaaagggc ggaaaagatc
     4261 attgagaagc tcatcaaaga agattacctc aatgaagaac gctttgccag gacctatgcc
     4321 ggcggcaagt tccggatcaa ccactggggc aagacgaaaa tcatttacga gctggaaaaa
     4381 agacaggtgc ccgacctgat catccagatc gggctccagg aaatcgacga ccaggaatat
     4441 aaagaaacgc tgaaggagat tctgactcgc aaagcaaggg aaatcaagga gaaggatccg
     4501 tatcggcgca ggcaaaagct aactgcttat gcggttcaga aggggtatga gtatcatttg
     4561 ataagagagg tggctgaaca gatgaaccgg tgaaacgaat cagcaaaacc ctaaggcatg
     4621 ttcatattta agttgtcaca gtatgtttct cggtctatct tgagatggca tctcaaatcc
     4681 atggatttaa ttcatatatc attctatatc ttctctttac aacgattttc cgtagatttg
     4741 agatgccatc tcaaaaaatc tcgctagcta ggtttaatcc ccttgagatt aataactatc
     4801 tttacctcat aatacaatct taaata
//
LOCUS       DAOQXS010000195         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17941_length_4815_cov_4.542017, whole genome shotgun sequence.
ACCESSION   DAOQXS010000195 DAOQXS010000000
VERSION     DAOQXS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17941_length_4815_cov_4.542017"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3202)
                     /gene="sprA"
                     /locus_tag="PKM34_06530"
     CDS             complement(<1..3202)
                     /gene="sprA"
                     /locus_tag="PKM34_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNQ83281.1"
                     /translation="MRRVGSNIFTFSLISLIVLLSWTIPASVDYGHTHGFDIPWFSPP
                     DTTVKGDTSVVLPFPPPKQDDYLLYQPEDTGGLYLRPPKNFKTEVEYDPSTNQYYFRN
                     KIGDLDYRNPTYMSFDEYQEYELDNTIKQYWRERSSPSSTLTKDGIIPSLYIGGQVFD
                     RIFGSNTIDIRPQGSAELSFGVLANRRDDPSLNVRQQRTTNFDFEQNIQMNVMAKIGD
                     KIQFNTNFNTEAVFDFENKLKLNYEGKEDEIIKLIEAGNVSMPLSTTLITGTQSLFGF
                     KTKLQFGRTTVTGLFSEQESESSTVVIENGKESNRFELKATDYEENKHFFISHLFRES
                     YDKALAELPIVASDINITRIEVWVTNIGPAIQQNRNLIAFQDLGEFARINNPNVNPIP
                     GRPYPSNNSNNLLQQLDVNQLRSISSVGTYLNGDPFSIGYTGYMVNGEDYEKIESARK
                     LETSEYSVNSKLGFISLYTTLNPDQSLAVAFQYTVIGVDSVFQVGEFSDQTSNTSNCL
                     VVKLLKSTSLSTQVPMWDLMMKNVYSIGAYRVSRDKFILNILYSGNSNGVPTGYFTEG
                     PEGIKGTPLIQFFNLDNLDQLLNPPPDGLFDFIDNAAVSGGTMNSSNGRLYFTVIEPF
                     GKFLRNKFGPGNTDLANKYAFDTLYRSTKTIAEQQTEKNKYLLEGFYSSESGAEIDLN
                     AFNIPRGSVKVTAGGRTLTENVDYTVDYTLGRVRIINEGILNSGLPINITNENQSTFN
                     VLKKRMMGLRVDHQIGKDIRFGGTLLNLTERPLTQKVDYGNDPISNTIWGLDGSYQGE
                     SRLITSIVDKLPGIETKAPSKVNVEGEFASFMPGHSKAIGKSGVVYIDDFEGAKSTID
                     LRNVGTWFLASTPQGQPDLFPEASGNDRSYGFNRAKAAWYTIDPLFYDRNNNLRPANI
                     SRNELARHSVREVLETEVFPNKEIPNGIPTNIPVFNLAFYPSERGPYNYDVDGLPGIS
                     AGIGQDGSLIDPASRWGGIMRKIESSDFEATNVEYIEFWMMDPFNSDAEQNNPGRLYI
                     NLGDISEDILKDGRKFYENGLPTSEIVTDVDTTIWGR"
     gene            complement(3293..3904)
                     /gene="ruvA"
                     /locus_tag="PKM34_06535"
     CDS             complement(3293..3904)
                     /gene="ruvA"
                     /locus_tag="PKM34_06535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ83282.1"
                     /translation="MYEYIQGKLAEINPAFAILDVGGVGYNLQITLNTYTRISNLDQC
                     RLYAHLVIREDAQVLFGFAETEERDLFRLLLTVSGIGPNTARLLLSSLNIVELKDAIT
                     AGRVNILKSVKGIGEKSAQRIIVDLRDKIDKGSSPVQKVDFSHNTIREESLSGLVILG
                     FPRKIAEKAVDDVIRELKQLTSEGSPTTVINVERIIKEALKRL"
     gene            4050..>4815
                     /locus_tag="PKM34_06540"
     CDS             4050..>4815
                     /locus_tag="PKM34_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNQ83283.1"
                     /translation="MDNLFSKQALEYHSQGRPGKLEVIPTKPYSTQKDLSLAYTPGVA
                     KPCLEIKANPDDVYKYTMKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGLLFKVFA
                     DLDVFDIEINEKDIDKFVQTVVAIAPTFGGINLEDIKAPECFEIEDRIKEALDIPVMH
                     DDQHGTAIITAAGLLNALEITGKKIENLKVVVNGAGASAISCTRLYLALGVKRENVTM
                     VDTKGVLRSDRTDLNKYKRFFATDRDVHTLEEALAGA"
BASE COUNT         1185 a         1132 c         1196 g         1302 t
ORIGIN      
        1 cccttcccca gatggtggta tcgacatcgg tcactatttc agaggttgga aggccgttct
       61 cataaaactt ccgtccgtct ttcaaaatat cctcagaaat atcgccgagg ttgatatata
      121 gtcttccggg attattttgt tcggcatcgc tgttgaacgg atccatcatc cagaattcga
      181 tatattccac attcgtcgct tcaaaatcgc tcgattcaat tttgcgcatg atcccgcccc
      241 accggctcgc aggatcgatc agggagccat cctgtccgat accggcagaa atacccggga
      301 gcccatccac atcatagtta taagggcccc tttcggaagg ataaaaagca aggttgaaaa
      361 ccgggatatt ggtcggaatc ccgttgggga tctccttatt cgggaagact tcggtttcca
      421 gcacctccct gacggaatgg cgggccagtt cattgcgtga aatattggcc ggcctcaggt
      481 tattattccg gtcatagaat aaaggatcga tcgtgtacca tgctgctttg gcccggttga
      541 atccatagct tctgtcgtta cctgaagctt cagggaacag gtcgggctgg ccctgcgggg
      601 tactggccag gaaccaggtg ccgacatttc gaaggtcaat ggtcgattta gccccttcaa
      661 aatcatcgat atacactacg ccgcttttac cgatagcttt ggaatgtccg ggcataaaac
      721 tggcaaactc accttcgaca ttaacttttg aaggggcttt tgtttcgatg ccggggagct
      781 tatcgacgat gctggtgatc agcctcgatt caccctggta acttccgtcc agtccccaga
      841 tagtatttga aatcgggtca tttccgtagt cgaccttttg tgtcagaggc ctctcggtca
      901 ggttcagcaa cgtacccccg aaccggatat ccttcccgat ctggtgatcg acccgtaaac
      961 ccatcatcct cttcttcaaa acattgaagg tcgactggtt ttcattcgta atattgatcg
     1021 gaaggcccga attgaggata ccttcattga tgatcctcac cctgcctagc gtatagtcaa
     1081 ccgtatagtc cacattttcg gtcagcgtgc ggccaccggc cgtcaccttc accgacccgc
     1141 ggggaatgtt aaaagcgttc agatcgattt ccgcacccga ctccgaagaa tagaatcctt
     1201 ccagcaggta cttgttcttt tccgtttgtt gttcggcgat ggttttcgtg gagcggtaaa
     1261 gcgtatcaaa agcatattta ttggccagat cggtgttacc gggcccgaat ttattcctca
     1321 ggaacttgcc gaatggctca atgacggtaa aataaagcct tccgttggat gagttcatcg
     1381 tacctccgct gacagcggca ttgtcaatga aatcgaacaa tccgtccggc ggtgggttga
     1441 gcagctgatc caggttatcc aggttgaaga actggatcag cggtgttcct ttgatccctt
     1501 ccggtccttc ggtaaaataa ccggtcggga caccgttgct gttcccggaa tagaggatat
     1561 ttaaaatgaa tttatcgcgg ctgacccggt aagcgccaat ggaataaaca tttttcatca
     1621 tcaggtccca catcggcacc tgggtactga gggaggtgct tttcagcagt tttacgacca
     1681 ggcagttgga ggtgttggat gtctggtctg aaaattcccc gacctggaaa acgctatcga
     1741 cgccgatgac cgtatactgg aaagccactg ccagcgactg gtcggggttc agtgttgtat
     1801 aaagcgaaat aaaaccgagt tttgaattga ccgaatattc cgaggtttct agtttccggg
     1861 cgctctcgat cttttcataa tcttccccgt tgaccatata acccgtgtaa cctatgctga
     1921 aagggtctcc gttcaggtaa gttccgaccg aactgatgct gcgcaactgg ttgacatcaa
     1981 gctgctggag caggttattg gaattatttg acggataagg ccttccgggt ataggattga
     2041 cattcggatt attgatgcgg gcgaattctc ccaggtcctg gaaggcaatg aggttacggt
     2101 tctgctggat ggccggaccg atattggtga cccaaacttc aatacgggtg atattgatat
     2161 cggatgcgac aatgggcagc tcggccaggg ctttgtcata actttcacgg aagagatgtg
     2221 agatgaagaa gtgtttgttc tcctcatagt cggtcgcttt cagctcgaac cggttcgatt
     2281 cctttccgtt ttcaatgacg accgtagaac tttcgctttc ctgttctgaa aacaggccgg
     2341 tcaccgtcgt ccggccgaac tgcagtttgg ttttgaatcc gaagaggctc tgcgttccgg
     2401 tgatcagggt cgtgctgaga ggcatcgaca cattcccggc ttcgatcagt ttaatgattt
     2461 catcctcttt tccttcgtaa ttaagcttga gcttgttttc aaaatcgaat acggcttcgg
     2521 tattgaaatt ggtattgaac tggatcttat caccgatttt ggccatcacg ttcatctgga
     2581 tattctgctc aaaatcgaaa ttggtggtcc gttgctgcct gacgttcagc gacgggtcat
     2641 cgcgccggtt ggccaggact ccgaatgaaa gctcggctga gccctgcgga cgaatgtcga
     2701 tggtattgct tccgaagatc cggtcgaaaa cctgtccccc gatgtaaagg ctcgggatga
     2761 tcccgtcttt ggtgagggtg ctgctgggcg acgacctttc tctccagtat tgtttgatcg
     2821 tattgtcgag ttcatactcc tgatactcat cgaacgacat ataggtcgga ttccggtaat
     2881 cgagatcccc gattttgttt cggaagtaat actggtttgt cgagggatcg tattcgactt
     2941 cggttttaaa attcttcgga ggcctgagat acagtccacc ggtatcttct ggctggtata
     3001 gcagataatc gtcctgtttc gggggaggga atggaagcac aaccgatgta tcgcctttta
     3061 ccgtggtatc gggaggtgag aaccagggaa tgtcaaaacc gtgcgtatgg ccatagtcca
     3121 cactggcagg gatagtccat gacaggagga cgatcaggct gatcaggctg aatgtaaaaa
     3181 tatttgatcc tactctccgc aaacgttatc ctggtttatg gcctgttaat aacaaaattg
     3241 aaaacccggg taaagttttc aatgtaagat acgatactgt ttaccgctat tttcaaagcc
     3301 ttttaagtgc ttctttaatg atccgttcca cattgatgac agtggtgggg cttccttcgg
     3361 aagtcagttg ctttaactcc ctgatcacat catccactgc tttttcggct atctttctcg
     3421 gaaaacctaa aataaccagt cctgataacg attcctcccg gatagtattg tgtgagaaat
     3481 caactttttg gacaggcgat gatcctttgt caattttatc ccttaagtca acaatgatcc
     3541 gctgtgccga tttctccccg attcctttga ccgatttgag aatattgaca cgtcctgccg
     3601 taatggcatc ttttaattca acaatattca gtgatgaaag aagaagccgt gccgtattgg
     3661 ggccgatgcc ggaaaccgtc agcagcagcc ggaacagatc tctttcttcc gtttcagcaa
     3721 atccgaagag aacctgtgca tcttcccgga taaccaggtg ggcatacaac ctgcattggt
     3781 caagattcga tattctggta taggtattca gggtgatctg aaggttatac cctactccgc
     3841 cgacgtcgag gatggcaaag gcgggattta tttcagccag ctttccttgg atatattcgt
     3901 acatatcagt tcgttccggg ctcttttttc ggagggcaaa aataggaatt taatttagaa
     3961 ctaatctgcc taaaccatca ataaagaaat aagatgaaaa tattatattt gctgattgtg
     4021 ttaatttctt aacaataaat taaaaaccaa tggataacct cttcagcaaa caagccctgg
     4081 aatatcactc ccaggggagg ccgggcaaac tcgaagtgat cccgaccaaa ccttacagta
     4141 cccaaaaaga tttgtcgctg gcctatacac cgggggtagc caaaccctgc ctggaaatca
     4201 aggccaaccc cgacgatgta tacaaataca ccatgaaagg gaacctggtg gcggtgatct
     4261 cgaacggaac agccgttctg ggcctgggcg atatcggccc ggaagccgga aagccggtca
     4321 tggaaggcaa aggattgctg tttaaagttt tcgcggatct tgatgttttt gatattgaga
     4381 tcaatgagaa agatatcgat aaatttgtcc agaccgttgt tgccattgcg ccaacctttg
     4441 gcgggattaa cctggaggat atcaaagcgc cggaatgttt tgagatcgaa gaccgcatca
     4501 aggaagcgct ggatattccc gttatgcatg atgatcagca tggaaccgca attattacgg
     4561 cagccggttt gctgaatgca ttggaaatca cgggtaaaaa gatcgaaaat ctgaaagtcg
     4621 tcgtcaatgg cgccggggca tcggccattt catgcacgag gctttacctt gcactgggag
     4681 tgaaacggga gaatgtgacc atggtcgata ccaaaggcgt gttgcgcagc gaccggaccg
     4741 atctgaacaa atacaagcgg ttctttgcaa cggacaggga tgttcataca ttggaagaag
     4801 cgctggcagg agccg
//
LOCUS       DAOQXS010000196         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_17994_length_4805_cov_3.475158, whole genome shotgun sequence.
ACCESSION   DAOQXS010000196 DAOQXS010000000
VERSION     DAOQXS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17994_length_4805_cov_3.475158"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKM34_06545"
     CDS             complement(<1..767)
                     /locus_tag="PKM34_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83284.1"
                     /translation="MKMHIKSRKTFFLLLLLAASCVLLLHACDETGDEIDYQPNVNSS
                     KDFVRAEDALFDIVNAFLKGIYDSLIAQNNYAYIDYCGVTYDPDENIMHFSYGAVNRM
                     CQDGKFRRGDFFAEFDGPLFEPGVKANLITDSLFVNDSLVFASMTFNNLGLNAMEKVQ
                     YGLNVISLSVAPRDSLSLRRIQITADLNLSWDQGSSTPEIHEDDYYLVTGTASGISSD
                     FTDFSVMIEEPLVNYVDCCWIISGTNLITVPSADIKQG"
     gene            complement(782..1273)
                     /locus_tag="PKM34_06550"
     CDS             complement(782..1273)
                     /locus_tag="PKM34_06550"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNQ83285.1"
                     /translation="MKNISIIVAIARNYAIGRNNQLLWHLSEDLKRFKKITLGKQVIM
                     GKLTYLSLPVRPLPNRQNVVITDRKDEVFEGCTTVHSIEEALDLCHEAEESFIIGGGS
                     VYRQFLPYCNRLYITLVHKDFDADTFFPQFDLAQWKLVEREDRLSDNDSELSYSFLIF
                     EKA"
     gene            complement(1270..2064)
                     /locus_tag="PKM34_06555"
     CDS             complement(1270..2064)
                     /locus_tag="PKM34_06555"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNQ83286.1"
                     /translation="MIQYLELLDHVRKNGVEKADRTGTGTLSVFGYQMRFDLQKGFPV
                     LTTKKLHLRSIIHELLWFLKGETNLKYLHENKVTIWDEWAGPNGDLGPVYGYQWRSWP
                     AGNGKHIDQISNVIHSIKTNPDSRRHIVSAWNVGELDKMALPPCHLLFQFYVAEGKLS
                     CQLYQRSCDIFLGVPFNIASYALLTLMVARVTGLQPGDFVHTLGDAHIYLNHIEQVML
                     QLSRKPYPLPKMNLNPERTNIFDFVFEDFELVDYQCHPHIKGEISV"
     gene            complement(2116..3534)
                     /locus_tag="PKM34_06560"
     CDS             complement(2116..3534)
                     /locus_tag="PKM34_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83287.1"
                     /translation="MKLKSLLLIFVMMIISSMPLESTAQTGQDVTQALSEAGAGFSIQ
                     SLLRGILGMMVLLGICYLFSTNRKAISWRVVIFGLLLQAVLAITVLKVPAIQHVIEFI
                     ARVFIKILDSSNAGSSFLFKSLVTNKVETGLITFAFQILPTIIFFSALTSVLFYYGII
                     QKVVWAMAWLLTKLLKISGAEGLVASSNVFLGQTEAPFLIKEYLPKMNRSEIMLVMVA
                     GMATMAGGVLAIYIGLLGGDDPVGKMIFAQHLITASVMAAPAAVVTSKMLVPQTEPIE
                     SDIKITKERIGSNILDAIANGTTSGLKLAVNVAAMLIAFIALIYFLNFIFGKIGDWTH
                     VNAWVADITGGQYSKISLQFFLGYLLAPLTWLMGVNSHDMTLVGQLLGEKIILNEMIG
                     YASLQNLISSGVFYDQKSVIIAVYVLCGFANFSSVGIQIGGISAIAPNQRKTLSDLGF
                     RALLGGGLSALLSATLVAIILG"
     gene            complement(3594..4178)
                     /locus_tag="PKM34_06565"
     CDS             complement(3594..4178)
                     /locus_tag="PKM34_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNQ83288.1"
                     /translation="MVRIRLEIVGMSYSQSQSGAYALILGEVKGNRRLPIIIGGAEAQ
                     AIAVELEKIKPTRPLTHDLFKNFADTYHIQVKEVIIDQFRQGVFHAKLVCMQNEKESL
                     IDSRTSDAVALAIRFKCPIYTYEKIMAEAGMIMDENANLSDTMTTPLEESSESSFKDL
                     NLAELEELLQKAVDREDYEKASQIRDEIKKRKKG"
     gene            4322..>4805
                     /locus_tag="PKM34_06570"
     CDS             4322..>4805
                     /locus_tag="PKM34_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNQ83289.1"
                     /translation="MKKLISLLSLFLLPLLTFASEANLVIPDAVKDQSILYWGFLITV
                     LGLLFGLYQFVQVKKLKAHHSMLEVAQVIYETCKTYLLQQGKFLMILFVFIGATVAFY
                     FGYLSEGGFGIGAVLMILGWTVVGILGSYSVAWFGIRMNTLANSRMAFASLEKKPIKL
                     L"
BASE COUNT         1307 a         1120 c         1077 g         1301 t
ORIGIN      
        1 ccttgtttaa tatcggccga agggactgta ataaggttgg tccctgaaat aatccagcag
       61 caatccacgt aatttaccag cggttcttcg atcatgacac taaagtcggt aaaatcggag
      121 gagataccgg aagcagtacc tgttaccaga tagtaatcgt cttcatgtat ttccggtgtt
      181 gaagacccct gatcccagct caggttcagg tctgccgtaa tctggatccg tcgcaggctt
      241 aaagaatctc ttggggctac cgataatgaa attacattga ggccgtattg aactttctcc
      301 attgcattta atcccagatt attaaaggtc atacttgcaa agaccaggct gtcgttcaca
      361 aacaaactgt cggtaatcaa attcgcctta acacccggtt cgaacaaagg gccgtcaaat
      421 tcagcaaaaa aatcgcctct cctgaatttt ccatcctggc acatacgatt aacagcacca
      481 taactaaagt gcataatatt ctcatccggg tcatacgtca ctccgcagta atcgatatag
      541 gcgtaattat tttgtgcgat cagggagtcg taaatacctt tcagaaatgc attgacgata
      601 tcaaaaaggg catcttcagc cctgacaaaa tcttttgagc tgttcacatt aggctgataa
      661 tcaatttcat cacctgtttc atcacaagca tgcaacaaaa gaacacatga tgcggctaat
      721 aagagcagca gaaagaatgt tttccgtgat ttaatatgca ttttcattaa tattccttcg
      781 tttaagcttt ctcaaaaata aggaaagaat aagataattc cgaatcatta tccgaaagcc
      841 tgtcctctct ttcaaccaac ttccattgtg ccaggtcgaa ctgcggaaag aaagtatcag
      901 catcaaaatc tttatggacg agggttatat aaagcctgtt gcaatagggc aagaactggc
      961 ggtagaccga tcctccgcca atgataaagc tctcttccgc ttcgtggcaa agatccagcg
     1021 cttcctcaat actatgaacg gttgtacaac cttcaaaaac ttcatctttt ctgtcggtga
     1081 taacaacatt ttgacggttt ggcaggggcc ttaccggtaa gctcagatac gtcagcttac
     1141 ccatgatcac ctgtttgccc agggttattt ttttaaaccg cttcaggtct tctgagaggt
     1201 gccaaaggag ctggttattg cgtccaatgg cgtaattccg ggcgatagcc acaatgattg
     1261 agatattctt cataccgaga tttctccttt aatatgagga tggcattggt aatctaccaa
     1321 ttcgaaatcc tcaaaaacaa aatcaaaaat attggtccgt tccgggttca gattcatttt
     1381 gggcaacggg taaggctttc ttgacaactg gagcataacc tgttctatat gattgagata
     1441 gatatgggca tctcccaagg tgtgcacaaa atctcccggt tgcaggcccg ttacacgggc
     1501 aaccattaaa gtcagcagtg cgtaagatgc gatattgaaa ggcacgccaa ggaaaatatc
     1561 gcagctacgc tggtagagct ggcacgacaa tttcccttca gccacgtaga actgaaaaag
     1621 aaggtggcag ggaggaagtg ccattttatc caattcgccg acgttccagg cgcttacaat
     1681 gtgcctgcgg ctatcgggat tcgttttgat ggaatgaatt acgttgctga tctggtcgat
     1741 atgttttcca tttccggcag gccatgaccg ccactggtag ccgtaaacag ggcccaggtc
     1801 accgtttgga ccggcccact catcccagat ggtcacctta ttctcatgca ggtacttcag
     1861 gtttgtttct cctttgagaa accagagcaa ttcatggatg atcgaccgaa ggtgcaattt
     1921 ttttgttgtc agtaccggga atcccttttg caggtcgaac cgcatctggt agccaaaaac
     1981 gctgagggta ccggtaccgg tcctgtcagc tttttcaacg ccgtttttcc ggacatggtc
     2041 gaggagttcg aggtattgga tcatgtttaa ggtgccctgg ctaaaatttc agttaataac
     2101 gacgattact gcgtctcatc ccaggatgat ggccaccagg gtggcggata agagtgccga
     2161 gagcccgcct ccgagtaaag ccctgaatcc caggtctgat aaagttttgc gctggttcgg
     2221 agcgatggcg ctgatcccgc cgatctgaat tccgacggat gagaaatttg cgaacccaca
     2281 taacacgtaa acggcgatga tcaccgattt ctgatcatag aaaacaccgc tggagatgag
     2341 gttctgcagg ctggcatagc caatcatttc attcaggatg attttttctc cgagaagttg
     2401 cccgacgagc gtcatatcgt gcgaattgac gcccattagc caggtcagcg gggccagcag
     2461 gtaaccgaga aagaactgca gactgatttt cgaatattgt cctccggtaa tgtcggctac
     2521 ccatgcattc acgtgggtcc agtcgccgat tttgccaaag ataaagttca aaaaatagat
     2581 aagtgcgata aaagcaatca gcatagcggc aacattaaca gccagtttta gccccgaggt
     2641 tgtaccgttg gcgatggcat ccaggatatt gctgccaatc ctttccttgg tgatcttgat
     2701 atcgctttcg atcggttcgg tttgcggcac gagcattttt gaagtcacca ccgcggctgg
     2761 tgcagccata acggaagctg tgatcaggtg ctgggcaaag atcattttcc ctaccgggtc
     2821 atcaccgccc agcaaaccga tatagatggc cagtacaccg ccggccatcg tcgccatccc
     2881 ggccaccatc accagcatga tctccgaccg gttcattttc ggcaggtatt ccttgatgag
     2941 gaaaggggct tcggtttgtc cgagaaaaac attgctcgaa gcgacaagcc cttctgcgcc
     3001 tgaaatcttt aagagcttgg tcagcagcca ggccatggcc caaacgacct tttgaatgat
     3061 accataataa aacaggacgc ttgtgagggc agaaaagaag atgatcgttg gcaggatctg
     3121 gaatgcgaaa gtgatcagcc cggtttccac cttattagtc accagtgatt taaaaagaaa
     3181 tgaactgccg gcattggatg aatcgagtat cttgatgaaa actctggcga taaattcaat
     3241 aacatgttgt attgcaggga ctttaagaac agtaattgcc aggacagcct gcaaaagcaa
     3301 cccgaaaatg accacccgcc agctgatggc tttgcggttc gtgctgaaaa gatagcatat
     3361 accaagcagc accatcattc ccaaaatccc cctgaggagt gactgtattg aaaatcccgc
     3421 cccggcttcg ctcagtgcct gtgttacatc ctgcccggtt tgggccgtgg attctaaagg
     3481 catgctgcta atgatcatca tgacaaagat caacagtaat gatttcagtt tcatcaaaat
     3541 agttcttatt aaaggtctaa tactggaaca gctttattca aaaagaaaaa aactcatccc
     3601 ttctttcttt tttttatttc atccctgatc tgggaggctt tttcataatc ttcccggtca
     3661 acagctttct gcaggagttc ttccagttcg gcaaggttta aatccttaaa cgaactttca
     3721 ctggattctt ccaggggagt ggtcatggta tcggataaat tggcattttc atccatgatc
     3781 attcccgctt cagccataat tttctcgtag gtataaatcg ggcatttaaa gcgaatagcc
     3841 aaagcgactg catcggaggt cctggaatcg atgagactct ctttttcatt ctgcatgcag
     3901 accagttttg catggaaaac accctggcgg aattggtcga tgatcacctc tttcacctgg
     3961 atatgataag tatcggcaaa atttttaaag aggtcatggg tcaaaggccg ggtaggttta
     4021 atcttttcca gctcaaccgc aatagcctga gcctcggccc ctccaatgat aataggtagc
     4081 cggcggtttc ctttaacctc accaaggata agggcataag ccccgctttg cgactgactg
     4141 taagacatcc cgacgatctc aagcctgatc ctgaccataa aaattcagtt aaccctttta
     4201 ccggataaaa atagaataaa tttcaagccg catatcccct gttgataaaa tagaattgcg
     4261 attttgaaat acttttttaa ttttgttttc ctcactcata caaaaccaaa aaataaccat
     4321 tatgaaaaaa ttaatctctt tactttccct atttctattg ccattgctga catttgccag
     4381 cgaggccaat ctggtaattc ccgatgcggt taaggaccaa tccattttat attgggggtt
     4441 cctgattacg gtactgggtt tactctttgg cctttaccag tttgttcagg tgaagaaact
     4501 gaaagcccat cattcgatgc tggaagtggc acaggtgatt tatgaaacct gcaaaacata
     4561 cttattgcaa caaggtaagt tcctgatgat cctgttcgtc tttatcggag ctacggtggc
     4621 cttttatttc ggttacctgt cggaaggcgg attcggaatc ggcgctgtgc tgatgatcct
     4681 gggctggacc gtagttggaa tcctggggtc ttactcggtc gcctggtttg gtatcaggat
     4741 gaacacactg gccaactcca ggatggcctt tgcctcgctg gagaagaaac ccatcaaatt
     4801 attga
//
LOCUS       DAOQXS010000197         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18045_length_4794_cov_4.112682, whole genome shotgun sequence.
ACCESSION   DAOQXS010000197 DAOQXS010000000
VERSION     DAOQXS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18045_length_4794_cov_4.112682"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKM34_06575"
     CDS             complement(<1..187)
                     /locus_tag="PKM34_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83290.1"
                     /translation="MKEHPEPFDQHLRDSLKGHRLTPSDEARKAFLQEAAAIVPDRKK
                     WYKWYYLPAVLVLIFSAI"
     gene            complement(188..763)
                     /locus_tag="PKM34_06580"
     CDS             complement(188..763)
                     /locus_tag="PKM34_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ83291.1"
                     /translation="MKLEKALIKDVLDNRSNAVEALYDRFAPAMLSLCFRYTGNIQDA
                     EDVLHEGFIKILKSLGDFKPRQEHSLEAWMKRIMTNTALNFLRDHSREKRILDIDSVR
                     EKDFILEDDDVGTDIYSLSVTQDQIMELIAALPLGYRTVFNLYVFEDHSHKEICEQLG
                     CSENTSKSQLRKARALLRARIIEMANLIKVK"
     gene            complement(893..2230)
                     /gene="gdhA"
                     /locus_tag="PKM34_06585"
     CDS             complement(893..2230)
                     /gene="gdhA"
                     /locus_tag="PKM34_06585"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNQ83292.1"
                     /translation="MNLEKIMNDLEKKHPGEAEYLQAVREVLESIEETVNENPQFEAA
                     GIIDRIVEPDRILTFKIPWVDDQGKVHVNLGYRVQYNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEIMRFCQSFMLELWKIIGPETDV
                     PAGDIGVGAKEIGFLYGMYKKLTHEHTGVLTGKGLNWGGSLIRPEATGFGAVYFAQEM
                     LSTRGMSIEGKTVCISGFGNVAWGAVTKVNHLGGKVVSISGPDGYIYDPDGISGEKIE
                     YMLELRASNQDIVKPYSYEFPNAQFHQGKHPWEVKCDVALPCATQNELNAEDARNLVK
                     NGCICVAEGANMPSTPDAVEIFIQNKLLFGPGKAVNAGGVATSGLEMSQNSMKLGWPR
                     NEVDARLHQIMRDIHASCVKHGTEPDGYINYVKGANVAGFLKVANAMIDQGVI"
     gene            complement(2397..3752)
                     /locus_tag="PKM34_06590"
     CDS             complement(2397..3752)
                     /locus_tag="PKM34_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ83293.1"
                     /translation="MALVVVLFLVSSGRFAFGQVEPKTHVVGAIDNFFSISIKYNVSV
                     EDLKKANPGITSPKPGDVLIIPTPNVATTPEKHIYKPNRPAGGDPFRIALMVPFYLEQ
                     LGDTSWRQKLETSSIPDIAPFRFVQFYHGFLMAADSLAKKGLNAEIYVYDVDQQVSKA
                     RHAIADPELKEMDLIVGPLLKGAFEEVAAFANDHHIPIVNPLSTRNDILVNNPYVFKL
                     SPSVESQPKLVADLIRRDFIDHNIIFYIPNRVQNTELIGRYSEAIEESIGKGRKEITV
                     IDYATDSIRGFQSHASTSRPNLVVIFSENEVLPAALLNKLNAMKSDYEMTVIGLPEWD
                     KFTTIELGYMMPLNAHVLMANYVDYRSDAIKGFVNAYRVKYYDEPLTYAFSGFDAGYY
                     FMGALMEFGKDFVNHTDEIRIPVIQNQFHFKRAGNDGFDNLNWNILYYYDFSLFRKYS
                     Y"
     gene            complement(3816..>4794)
                     /gene="guaA"
                     /locus_tag="PKM34_06595"
     CDS             complement(3816..>4794)
                     /gene="guaA"
                     /locus_tag="PKM34_06595"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ83294.1"
                     /translation="VGICGCSQSWTPSLFIESTVHELQEMLGDDKVVLGLSGGVDSSV
                     AAVLLDKAIGKNLHCIFVDNGLLRKNEFESVLHSYKDMGLNVKGVDARQRFYMALQGI
                     SDPELKRKAIGRTFIEVFDEEAHHIRDVKWLAQGTIYPDRIESASVKGPSATIKSHHN
                     VGGLPDYMKLKVVEPLHSLFKDEVRRIGTALGMKTDLLNRHPFPGPGLGVRILGEVTP
                     ERIRILQEVDEIFISGLKQYGLYDSVWQALAVLLPVNSVGVMGDERTYENAVVLRAVG
                     STDGMTADWSQLPYDFLGKMSNEIINRVKGVNRVVYDISSKPPATIEWE"
BASE COUNT         1208 a         1239 c         1085 g         1262 t
ORIGIN      
        1 caatggctga gaagatcagc acgaggacgg ccggaaggta gtaccatttg taccactttt
       61 ttctgtccgg aacaatggcg gcagcttcct gcaggaacgc tttcctggcc tcgtcactgg
      121 gtgtgagccg gtgccctttc agcgaatccc tgagatgctg gtcgaatggt tcgggatgct
      181 ctttcattta tttcacttta attaagtttg ccatttcaat gatccttgcc cgaagtaaag
      241 cacgggcttt tcgcagctgg gatttggatg tgttttccga gcagcccagc tgctcgcaga
      301 tttccttatg ggaatgatcc tcaaaaacat acaggttaaa tacagtgcgg taccccagtg
      361 gcagggcggc gatcagttcc atgatctggt cctgcgtaac ggataaactg taaatatccg
      421 ttcccacgtc atcatcttcc aggataaaat ccttctccct gacggaatcg atgtcaagga
      481 ttctcttctc ccttgaatgg tcgcgaagaa aattcagcgc cgtattggtc atgatccgtt
      541 tcatccaggc ctccagcgaa tgttcctgcc gtggtttaaa atccccgagg cttttcagga
      601 tcttgatgaa gccttcatgc agtacatcct cggcatcctg gatatttccc gtataacgga
      661 agcaaaggct cagcatggca ggcgcaaacc ggtcgtacag cgcctctacg gcgttggaac
      721 ggttatccag cacatccttt attagcgcct tttctagctt catcaacaaa tccgctgaga
      781 ttaaaacacc atcgccctga atggttgcct gtaaatttaa aaaaatacca atgaaaaaga
      841 ggcttctcca ctctgtgaga aagcctctct taaaaagatt atgtcagtca gattaaatga
      901 cgccctggtc gatcatggca ttggctactt taaggaagcc ggccacgttg gcgcctttaa
      961 catagttgat atatccgtcg ggttcggtac cgtgttttac gcaggaagca tggatgtcgc
     1021 gcatgatctg gtgcaggcgg gcatcgactt catttctcgg ccagccgagt ttcatggagt
     1081 tctgcgacat ttcaaggcct gaggtggcta caccaccggc attgactgct tttccgggtc
     1141 cgaacaggag tttgttctgg ataaagattt caactgcatc gggagtggag ggcatattgg
     1201 cgccttcagc gacgcaaata catccgttct tcaccaggtt gcgggcatct tctgcattca
     1261 gctcattctg tgtagcgcag ggcaatgcca catcgcactt cacttcccac gggtgcttgc
     1321 cctggtggaa ctgggcattc ggaaactcat acgaataagg ttttacgata tcctggttcg
     1381 atgcacgcag ttcaagcata tattcgatct tttcacctga aatgccatcc ggatcataga
     1441 tataaccatc aggtccggag atggacacga cttttccgcc aaggtggttc actttagtga
     1501 ctgcacccca ggccacgttt ccgaaaccgg agatacaaac cgttttacct tctatcgaca
     1561 ttccacgggt tgaaagcatt tcctgtgcga aatacacggc tccgaatccg gtggcttccg
     1621 gacggataag gcttccgccc cagttcaatc ctttaccggt cagaacaccc gtatgctcat
     1681 gggttagttt cttgtacatc ccatagagga atccgatctc tttggctcca acaccaatat
     1741 cacctgccgg cacatcggtc tcgggtccga taatcttcca aagttccagc atgaacgact
     1801 ggcagaaacg catgatttca ccgtctgatt tgcctttcgg gtcaaaatca gaaccccctt
     1861 tgccaccacc catcgggagc gtggtcaggg cattcttgaa tatctgctca aatcccagga
     1921 acttcaaaat gctcaggttc acactcgggt ggaaacgcag cccgccctta taaggcccga
     1981 tagcattgtt gtactgaaca cggtaaccga gattgacatg caccttgccc tgatcgtcaa
     2041 cccacggaat cttgaatgtt aaaatccggt caggttccac aatccggtcg atgatcccgg
     2101 cggcttcaaa ctggggattc tcatttaccg tttcttcgat cgattccaaa acctcgcgta
     2161 cagcctgcag gtattcagct tcacccgggt gtttcttctc gaggtcgttc attatcttct
     2221 ctaaattcat gatgtaaaga tttatagttt aattatgtta aaatttaaca ctgttaaaaa
     2281 attaacagga caaaattagt gctttatctt atacaaaaaa gaaaaatggc tgaagaatca
     2341 aataattttt tgaaaagagc agaatagttg aaaaaccagt ccgaattcgg tctaaatcaa
     2401 taggaatatt tccggaaaag tgaaaaatca taataataca gaatattcca gttaaggtta
     2461 tcgaaaccat cattgcctgc ccgcttgaaa tgaaactggt tctggataac ggggatacgg
     2521 atttcatccg tatggttaac aaaatctttc ccgaattcca taagtgcccc cataaaataa
     2581 tagccggcat cgaatcctga aaaagcatag gtcagcggct catcgtagta tttcaccctg
     2641 taggcattta caaatccttt tatggcatcg gaacggtaat cgacataatt ggccatgaga
     2701 acatgcgcat tgagcggcat catatacccc agctcaatcg tggtaaattt atcccattcg
     2761 ggcaacccga tcaccgtcat ctcataatcg gatttcatgg cattcagctt attcagcaat
     2821 gctgccggca ggacctcatt ctcagaaaaa atgacgacca ggttagggcg tgaggttgaa
     2881 gcatggctct gaaaaccgcg gatgctgtcg gtagcgtaat cgatcaccgt tatttccttt
     2941 cttcctttac ctatcgactc ttcgatcgct tccgaatatc ttccgatcag ctctgtgttc
     3001 tggacacggt ttgggatata aaagatgatg ttatggtcga tgaaatcgcg ccgtatcaga
     3061 tctgctacca gtttcggctg tgattctact gatggcgaca atttgaagac atagggatta
     3121 ttcaccagga tatcatttct cgtagagagg ggattgacaa taggaatatg atggtcattg
     3181 gcgaatgcag caacctcttc aaatgctccc ttaagaagag ggccgacaat gaggtccatc
     3241 tccttcagct cagggtcggc tatggcatgc cgggctttgg atacctgctg atcgacatca
     3301 tagacataaa tttcggcatt caatcccttt tttgccaggg agtcggcagc catcaggaaa
     3361 ccatggtaaa actgaacaaa acggaatggc gcaatatcag ggatgctgga ggtttcaagc
     3421 ttttgcctcc aactcgtatc gccgagctgt tccaggtaaa aaggcaccat cagcgctatc
     3481 cggaaaggat ctccccctgc cggcctgttc ggtttgtaaa tatgcttttc gggcgtcgtt
     3541 gccacattag gtgtcgggat gatcagcaca tcgcccggtt tcgggctggt aatccccgga
     3601 ttggcctttt tcaggtcttc aacagacaca ttgtatttga tcgaaatact gaaaaaatta
     3661 tcgattgctc cgaccacatg cgtcttcggt tcaacttgcc cgaaagcaaa ccggccggat
     3721 gataccagaa ataaaacaac aacaagcgcc aaaaggcccc ttttaattaa tttcctgata
     3781 tccataactt tccgtatttt ggttaatgat caagcttatt cccattcaat ggtggcagga
     3841 ggtttggaac tgatgtcata aaccactctg ttcacccctt tcaccctgtt gatgatctca
     3901 ttcgacattt tacccaggaa atcgtacgga agctggctcc agtcggctgt catgccatcg
     3961 gtcgacccga cggccctcag caccacggca ttttcatagg tcctttcatc tcccatcacc
     4021 cccaccgaat ttaccggtag cagaacagca agagcctgcc agacactgtc gtacaaaccg
     4081 tactgtttca gaccgctgat aaagatctcg tcgacctcct gcaaaatcct gatccgttcc
     4141 ggggtcactt cacccagaat cctgacgccc aggcccggtc cgggaaacgg gtgacggttc
     4201 agcaggtcgg tcttcattcc cagcgcggtg ccgattcgcc tgacctcgtc tttgaacagg
     4261 gagtgcagcg gttccaccac cttcagcttc atataatcgg gcagtccgcc gacattatgg
     4321 tgcgacttga tcgttgccga tggccctttg acagatgccg actcgatgcg gtcgggatag
     4381 atcgtgccct gggccagcca tttcacatcg cggatatgat gcgcctcttc atcaaaaacc
     4441 tcgatgaagg tcctgccgat ggcttttctt ttaagttccg ggtcggagat gccctgcagg
     4501 gccatataga accgctgcct cgcatccacc cctttgacat tcagccccat atccttgtac
     4561 gaatgcagga ccgattcgaa ctcatttttc ctcagcaggc cgttatcgac aaatatgcaa
     4621 tgcaggtttt tgccaatagc tttatcgagc aacacggctg ccacagagga gtcgacccct
     4681 cctgacagcc cgaggacaac tttatcatca ccaagcattt cctgcaattc atgcacggtt
     4741 gattcgatga acaggctggg agtccaggat tgagaacatc cgcatatccc aacg
//
LOCUS       DAOQXS010000198         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18066_length_4788_cov_3.248257, whole genome shotgun sequence.
ACCESSION   DAOQXS010000198 DAOQXS010000000
VERSION     DAOQXS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18066_length_4788_cov_3.248257"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3812)
                     /locus_tag="PKM34_06600"
     CDS             complement(<1..3812)
                     /locus_tag="PKM34_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83295.1"
                     /translation="MRIKLRLILVAAITVLFNNLQAQVEKPEETYILRPLSAVDEVQL
                     MSLPELKPPAGFENRDLPYMVDNSTQMYMRPAYQQAGLSCGQASCIGYNFTYEMARER
                     NLNASLLQNQYPTHFAWNFMNGGEGWYGVSYLHSIQILKEYGMMNAVDYGGSLSYGGA
                     SRFLSGYNEYYNGMHNRITNAYQIQVGTPEGLLVLKHWLHNHLEGSAVGGVASFYAQH
                     MAVTTMLPWGTPEAGKYVLTYFGGSANHAMTIVGYNDSIRWDYNSDGLYTNNIDINGD
                     GTVNMKDWEIGGFKMVQSYGGVPNWGDQGFAYMMYKTVADNLGSGGIWNHCVHVLDVK
                     GALDPKLTAKIILKHDRRERIKVLAGIANNVGAQRPEHQLGFPIYDYQGGNRYMQGGT
                     TEADKTIEIGLDLSKLLSYSDIGDPVKIYLQVVENDPDNANTGEVIHFSIFDHTSGGS
                     QVVCAQTNVPLVNDDTTTLYLNHTFNFDRVHIVDETIPPAPEGEYYSHQFTVAGGTPP
                     YIWEFDKSYDESGQPASFPQITGTQLTPGNSSSGYVTQAIPFSFPFYDSAYSSITLHV
                     DGYLMFDQQLYPFPYYQDDKVLFNITRHISPFLNYHQEINAGQGCGIWYEGSADYVTF
                     RWKTALTENPAMTFNYAARLYPDGTIKFYYGQMNGCDMYLWMAGIGDGDGFNFQETRL
                     SNKPSVNANTSTTLVPYDYPEEMTLSETGLFSGTPEQSYGNEQITFKVTDNNFIQTKK
                     TFTFTSTGIIISDSVISGGDPVIAFSETANMSISVLNLKPETIPDAVMTITIDDPYIT
                     VTDNTENLGDLAFNLPRNVLNAFQFTVASDIPNERLIIVETSITSDTNVWISELYHYA
                     YAPVVTAENVEVNDENGRLDAGETSDVVVSYLNTGGVELTSVNTLLTTADPLVMINQS
                     VGNIPLLEVGILANLSYNVFVSPDCLPGHEIEFALNISGDGNYATTDTFSLVVGLYSE
                     NFESGDMELFTWGHEGDGQWRTDTYAPYEGTFAARSGNITHNQESVMVIDMDYLTDGV
                     ISFYQRTSCEGDTTSADNYDYLSFKIDGNEMERWDGETGWSYAEFPVTEGFHRFEWIY
                     HKDHNTNYRMDAAWVDLISFASSVNCSPGLQFSPSSVDFLLRTGETETKSLALSNPAS
                     GPLNFTADIAGIQPSVVKDGKGNRNIEGSYLVTDHDRFHAGQEYVWNFRTYNAGNDNE
                     WIKQIYITFPQGIMLVAATDFTGGSGGPMVFQGPFGNGVTAHWFGEDANGWGVVHMGE
                     LAASDVSVV"
     gene            4196..>4788
                     /locus_tag="PKM34_06605"
     CDS             4196..>4788
                     /locus_tag="PKM34_06605"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNQ83296.1"
                     /translation="MFEKIKEAAGYLRSKGMVAPEVGIILGTGLGKLADHIDVEITID
                     YEDIPHFPISTVEFHHGVLIYGNLRGRKVLVMKGRSHAYEGYTTQQITFPIRVMKMLG
                     ISSLLVSNACGSMNPAFKKGSLMLIEDHINLLPNPLVGPNIDELGTRFPDMSQAYSRE
                     INAKITAIAERNGITLHKGVYVAVTGPTLETRAEYRF"
BASE COUNT         1084 a         1202 c         1279 g         1223 t
ORIGIN      
        1 accaccgaca catcactggc ggccagttcg cccatatgga cgacgcccca gccgttggca
       61 tcttcgccaa accagtgggc cgtcaccccg ttgccgaaag gcccctggaa aaccatcggg
      121 ccgcctgagc cacccgtgaa gtcagtcgcg gcgacgagca taatcccttg cggaaaagta
      181 atatagatct gcttgatcca ttcattgtcg ttgcccgcgt tgtaagtcct gaaattccag
      241 acatattcct ggcccgcatg gaaccggtca tggtctgtga ccaggtacga tccttcgata
      301 ttccggttcc cttttccgtc tttcaccacg gaaggctgta ttcccgctat gtcggccgtg
      361 aaattcaacg gccctgaggc cggattcgac aaagccagcg atttcgtttc ggtctcaccc
      421 gtccggagca ggaaatcgac cgatgagggg ctgaattgca agccgggtga gcaattgacc
      481 gacgatgcaa acgagatcag gtcaacccac gcagcatcca tccggtaatt ggtgttgtga
      541 tctttatgat agatccattc gaaccggtga aagccttccg tgacgggaaa ttctgcatac
      601 gaccaacccg tttcgccatc ccatctctcc atctcatttc catcaatttt aaaactcagg
      661 taatcgtaat tatcggcgga agtcgtatcc ccttcacaac tggtccgctg gtaaaaactg
      721 atgacaccat ccgtcaaata atccatatcg atgaccatca ccgattcctg attgtgtgta
      781 atattgcccg agcgggctgc gaatgtaccc tcgtaagggg cataggtatc agtcctccac
      841 tgcccgtcgc cttcgtgccc ccaggtgaac aactccatat caccggattc aaagttttcg
      901 ctgtataatc cgacaaccag tgagaatgtg tcggtagtcg catagttccc gtcaccgctg
      961 atattcagcg caaattcgat ttcgtggccc ggcaggcaat cgggcgaaac gaagacgttg
     1021 taactgagat tggccagtat tccgacttcc agcaacggga tattaccgac cgactggttg
     1081 atcatgacca gcggatcggc cgtcgtcagc aatgtattga cactggtaag ctctacgcct
     1141 cccgtgttca ggtaggaaac tacgacatcg gaggtttcgc cggcatccag cctgccattt
     1201 tcatcgttta cttctacgtt ttcggctgtt acgacgggag cataggcata atggtacaat
     1261 tcgctgatcc agacgtttgt gtcagaagtg atcgaggttt caacaatgat caggcgctcg
     1321 ttgggaatat cgctggcgac ggtaaactgg aatgcgttga gaacattccg cggcaggttg
     1381 aacgccaggt cgcccaggtt ttccgtattg tcggtaacgg taatgtaagg atcatcgatg
     1441 gtgatggtca tgaccgcatc gggaatggtt tccggcttca ggttcagcac cgagatgctc
     1501 atattggcgg tttcgctgaa tgcgataacg ggatcgccgc cagaaatgac agaatcgctg
     1561 atgattatgc cggtagatgt aaaagtgaag gtcttttttg tttggatgaa gttgttatcg
     1621 gttactttga atgtgatctg ttcattgccg taggattgtt caggcgttcc cgaaaaaagc
     1681 cccgtttcgc tcaacgtcat ctcctccgga tagtcatacg ggaccaatgt agtggaggta
     1741 ttggcattga ccgacggttt gtttgataac cttgtctcct gaaagttgaa tccatctccg
     1801 tcgccgatgc cggccatcca gaggtacata tcgcagccgt tcatctggcc gtagtagaat
     1861 tttatggtcc cgtcgggata gagacgggct gcatagttga aagtcatcgc cgggttttcg
     1921 gtcagggccg ttttccagcg gaaagtcacg taatctgcgc tgccttcgta ccagatgccg
     1981 cacccctggc cggcattgat ctcctggtgg tagttcagga aaggggagat gtggcgggtg
     2041 atgttgaaca gcaccttgtc gtcctgatag taagggaaag gataaagctg ctgatcgaac
     2101 atcaggtaac cgtcgacatg cagcgtgatg ctggaataag cgctgtcgta gaaaggaaag
     2161 gagaacggaa tggcctgggt gacgtacccg ctggaactgt tgccgggcgt caattgtgtt
     2221 ccggtgattt gcgggaaaga ggccggctgg cctgactcat cgtaactttt atcaaattcc
     2281 cagatgtaag gaggggttcc gcccgccacg gtgaactgat ggctgtaata ttctccctcc
     2341 ggggccggcg ggatcgtttc gtcgacaatg tggacgcggt cgaaattaaa ggtgtggttc
     2401 agataaagcg ttgtcgtatc atcgtttacc agcggcacat tcgtctgcgc acaaacgacc
     2461 tgggagccgc ctgatgtgtg gtcaaagatg gaaaaatgga tgacttcgcc tgtattcgca
     2521 ttgtcgggat cattctcgac aacctggagg taaatcttaa ccggatcgcc gatatcggaa
     2581 taacttaaga gtttcgacag gtcgagcccg atttcgatgg tcttgtcggc ttcggtggtt
     2641 cctccctgca tgtaccggtt gccgccctgg tagtcataga tcgggaagcc cagttggtgc
     2701 tcgggtcttt gtgcaccgac gttattggca ataccggcca gaacttttat ccgttcccgg
     2761 cggtcgtgct tcaggatgat ctttgccgtc agtttcggat cgagggcccc tttgacatcg
     2821 aggacatgga cgcagtgatt ccagataccg ccgctgccca ggttatcggc cacggttttg
     2881 tacatcatgt aagcaaatcc ctggtcgccc cagttcggga caccgccgta actctgcacc
     2941 atcttgaacc ctccgatctc ccagtctttc atgtttaccg tcccatcccc gttgatgtcg
     3001 atgttattcg tgtacagccc gtcgctgttg tagtcccagc ggatgctgtc gttgtaaccg
     3061 acgatagtca tggcatggtt ggcgcttcca ccgaaatagg tcagtacata tttgcctgcc
     3121 tcgggtgttc cccagggcag catggtggtg acggccatat gctgggcata aaagctggcc
     3181 acaccaccca cagccgatcc ctccaggtgg ttgtgcagcc agtgtttgag caccagcagc
     3241 ccttccggtg tgcccacctg gatctggtat gcatttgtga tccggttgtg catgccgttg
     3301 tagtattcgt tgtagcccga caggaatcgg gatgctccgc cgtatgataa agagccgccg
     3361 taatcaacgg cattcatcat cccgtattct ttcaggatct ggatgctgtg gagatagctt
     3421 acaccgtacc atccttcgcc gccgttcatg aagttccagg caaaatgcgt ggggtactgg
     3481 ttttggagca ggctggcatt cagattgcgt tcccgcgcca tctcataggt aaaattgtac
     3541 ccgatacatg atgcttgtcc gcagctgagt ccggcttgct gataggcggg acgcatatac
     3601 atttgtgtag aattgtcgac catgtagggc agatcacggt tttcaaatcc tgcaggcggt
     3661 ttcagctccg gaagcgacat cagttgcacc tcatctactg cagatagcgg ccgtaggatg
     3721 taggtctcct ccggcttttc tacctgggct tgaagattat taaataagac cgtgatagcg
     3781 gctaccagga taagtcgaag tttgattcgc atagtttttt attgaggctt taaagataga
     3841 taaaattaca tttaaggttg aaggttgagt gtttgattgt aattcgtgct gaagctgtct
     3901 caaaagtctg ttatttcgat ttgtttgcct gaaaacagtt cgtgctccaa cgactaaagc
     3961 cgttggttcc gaatgtttta tgagggttgg aaccgacggc tttagtcgtc ggactacaaa
     4021 gtttccacaa taaccgatgg gtttgcccgt cggaagaaaa aaacattaac cgacggtttt
     4081 agtcgtcgga ctgcttagtt tccacaataa ccgacgggtt cacccgtcgg actatcaagc
     4141 tcccttcgga gtgcaatctg tttttttttt aacttcgtcg cctcaattga atagcatgtt
     4201 tgagaagatt aaagaagcgg ccggatatct gaggtcaaaa gggatggtgg cgcccgaggt
     4261 gggaataatc ctcggcaccg ggctgggcaa actggccgat catatcgatg tggagataac
     4321 cattgattat gaggatattc cccattttcc catttccact gtcgaatttc accacggagt
     4381 tttgatatac ggtaacctgc gggggagaaa agtgctggtg atgaaaggcc gttcgcatgc
     4441 ttacgaaggg tataccacgc agcagataac ttttcccatt cgggtcatga aaatgctggg
     4501 aatcagcagc ctgctggtct ccaacgcatg cggttcgatg aacccggctt tcaaaaaggg
     4561 ctcgctgatg ctgatcgagg accatatcaa cctgttgccg aatccgctgg tggggccgaa
     4621 tatcgatgag ctgggtacac ggtttcccga tatgagccag gcctattcca gggagatcaa
     4681 tgcgaagatc accgccatcg ccgaaaggaa cgggatcacg ttgcacaaag gcgtctacgt
     4741 agccgtcacg gggccgaccc tcgaaacccg ggccgaatac cggttctt
//
LOCUS       DAOQXS010000199         4768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18168_length_4768_cov_4.141099, whole genome shotgun sequence.
ACCESSION   DAOQXS010000199 DAOQXS010000000
VERSION     DAOQXS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18168_length_4768_cov_4.141099"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKM34_06610"
     CDS             complement(<1..671)
                     /locus_tag="PKM34_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83297.1"
                     /translation="MKKSKVYLISLAVLLLIVIIWIATGSGPEKETTLEVPVKSGLFD
                     ITVTTTGELEAKSSEKIMGPVNLRDFRIWQIKIEDIIPDGTVVDSGQYIATLDRTELV
                     NKMKDQELEIEKLETQFTKTQLDTTLELRSARDNLINLKYALEEKQIVVDQSIYEPPA
                     TQRQAKIDLERAMRNYEQAVENYEVKLRKAEADMQEINTSMKKVRSEYAKMVSLLNEF
                     TIFAP"
     gene            complement(696..1664)
                     /locus_tag="PKM34_06615"
     CDS             complement(696..1664)
                     /locus_tag="PKM34_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ83298.1"
                     /translation="MISYKSDVEAVDAFVEKYGALRAEIAKVIVGQDEVVKRVLISIF
                     SRGHGLLVGVPGLAKTLMVNTIARALGLSYNRIQFTPDLMPSDIIGTEILDEERKFKF
                     IHGPVFANIILADEINRTPPKTQSALLEAMQEKAITVAGKNYKLPEPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIWLDYPTFEQEIEVVRSTTQDIPVDLKVILSAEEILFFQG
                     LIRRIPVPDNVYKYAVSLSAKTRPGTPAAHPLANDFVIWGAGPRASQYLIIGAKCHAA
                     IQGKYSPDIEDVRAVASSILRHRIVRNYKAEAEGYPIEKIMMEFLK"
     gene            complement(1694..3088)
                     /locus_tag="PKM34_06620"
     CDS             complement(1694..3088)
                     /locus_tag="PKM34_06620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ83299.1"
                     /translation="MKARRLYAFRGILFLGMILTGLPMTGIGQDEIVVDRIVAIVGKN
                     MILESDLESQYLQLRMQGEIEGSASSVKCQILQKMLRDNLLLNQAELDSVEVSDSDVE
                     QNLDRRLRYFISQFGSQEKLEQYYEKSLLEIKEEFRQMVKDFMLIEQVQNDITKNVFV
                     TPNEVKAFYGTIPSDSLPLVSARVEMTQIIKMPPVSAEQKVMIKERLRDLRRRVMAGE
                     NFATLAILYSEDPASAAKGGEIGFFGRGELYPEYEAAAFKLKEGEISDIIETKAGFHI
                     IQLMERKGDYINTRHILLQTKPSPEDLEKARLELDSLLLQIRNGEITFDDAVVKFSDD
                     PGKVSGGFIINDYDGTTWFPMDQLDPQVSFTVNKLEVGEISSPVPTQTEEGKEAFRLI
                     KLKSRSEPHRANLVDDYSMITNWALENKKEQKIVEWVNKNVDNAYIMIIDDYTDCDFE
                     NKWFPGKKQENENR"
     gene            complement(3146..3985)
                     /locus_tag="PKM34_06625"
     CDS             complement(3146..3985)
                     /locus_tag="PKM34_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83300.1"
                     /translation="MRKIVLLILPALVACTGLFRADDDDRVVARANDKELRFSEVAEK
                     IPDGLPSRDSITLAENYISKWIQNEILTRKAKETLKSNEQDFDKQLEEYRNSLLLFSL
                     EQKLVSQFLDTVITQQEIEAYYIENRSQFELKGNIVKFDFVKINKRSRHAREFRNLLK
                     ADKDESMMRLADYAEKNATDYWFAGEWVSFNDLLDEMPLEVDNQELFLRRNSYAEATD
                     SLFIYFVRINDFKTSDSISPLEFEKDKIRNIILNARKIDLIERKRQEFIDQAFKEDKA
                     KIF"
     gene            complement(3996..>4768)
                     /locus_tag="PKM34_06630"
     CDS             complement(3996..>4768)
                     /locus_tag="PKM34_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83301.1"
                     /translation="FMKCAMIGTGMKKPVFSIDKLKYSQQDLATYLASKQTKSVTSIR
                     GFFNDTYKSFVKEKCMAYEDSRLEQKYPDFRLLIREYHDGILLFDLMERNVWSKAVKD
                     TAGLAAYYGANKDKYMWDERFHATVVTVLNPGDVNIEELRDFITEGKPLEEILERYNS
                     DTTLNILAETEKYSRGDSPIIDKVQWKTGLSPMIDSPSGPAFVYGIAVAPPEPKLLQE
                     ARGLVTADYQSFLEEKWIGELRSKYPVTINQEVLSTLK"
BASE COUNT         1072 a         1186 c         1120 g         1390 t
ORIGIN      
        1 ttcggggcga atatggtaaa ctcgttcagc aggctcacca ttttagcgta ctccgaccgg
       61 acttttttca ttgaagtgtt gatctcctgc atatcggctt ccgctttacg cagctttact
      121 tcgtaattct ccaccgcctg ttcataattg cgcatagccc tttccaggtc gatctttgcc
      181 tgcctctgcg tagccggcgg ctcataaatc gactggtcga caacgatctg cttctcttcc
      241 agggcatatt tgagattgat gaggttatcc cgtgccgaac gaagctcaag cgttgtatcg
      301 agctgtgttt tggtgaactg cgtttcgagc ttttcgatct ccagttcctg gtctttcatt
      361 ttattgacca gttctgtccg gtcgagtgtg gcaatgtact gacccgaatc gaccaccgtt
      421 ccgtcgggaa tgatgtcttc gatttttatc tgccagattc gaaaatcgcg caggttgacc
      481 ggccccatga tcttttcgga gctcttggct tcgagctcgc cggtagttgt gaccgtaata
      541 tcaaagaggc ctgatttgac gggcacttcc agggtggttt ctttttccgg gccgctgccg
      601 gtggcgatcc agatgatgac aatcaacagg agaacggcca ggctgatgag gtaaactttg
      661 ctttttttca tgacagccgg tgttaggatt aggctttatt tcaaaaattc catcataatc
      721 ttttcgatcg gatatccttc cgcttcggct ttataatttc taacgatgcg gtgtcgcagg
      781 atggaggaag ccaccgccct gacgtcttca atgtcgggcg aatattttcc ctggatggcg
      841 gcatgacatt tggccccgat gatgaggtac tgtgatgcgc gaggaccagc tccccatata
      901 acgaagtcgt ttgcaagagg atgtgcggcg ggcgttccgg ggcgcgtctt tgccgacaag
      961 gaaacagcgt acttgtaaac attgtcgggt accgggattc ggcggatcag gccctggaaa
     1021 aagagtattt cctcggcgct gaggatcact ttaaggtcga cagggatatc ctgtgtggta
     1081 ctcctgacca cttcgatctc ctgctcaaat gttggataat ccagccagat attgaacata
     1141 aaccggtcga gttgcgcttc cggcagcgga taggtccctt cctgctcgat cgggttctgg
     1201 gttgccagta caaaaaatgg ttccggtagt ttatagttct ttccggcaac agtaatggcc
     1261 ttctcctgca tcgcttcaag cagagccgat tgtgttttgg ggggcgtcct gttgatctcg
     1321 tcagccagga tgatattggc aaaaaccggc ccgtgaatga atttaaactt ccgctcctcg
     1381 tccaggatct cggtcccgat gatatccgat ggcatcaggt cgggtgtaaa ctgaatgcgg
     1441 ttatagctta gtcccaaagc ccttgctata gtattgacca tcagtgtttt tgccaggccc
     1501 gggacaccaa ccagcaatcc atgtccacgg ctgaatatcg aaataagcac ccgtttgacg
     1561 acctcatcct ggcccactat cactttggca atctccgccc gaagggcgcc atatttctct
     1621 acgaaagcat caacagcttc aacatcagat ttataagata tcatcgtatt atttttccgg
     1681 gattgaaaat cacttacctg ttttcattct cctgtttttt accaggaaac catttatttt
     1741 caaaatcgca gtccgtgtaa tcatcgatta tcatgatata agcgttgtcg acattcttat
     1801 taacccattc cacgattttt tgttcttttt tattttcgag cgcccagttg gtgatcatgc
     1861 tgtaatcatc caccaggtta gcgcggtggg gctccgacct ggattttagc ttgataagcc
     1921 ggaaagcctc ttttccctcc tccgtttggg tgggtaccgg tgaagagatt tccccgacct
     1981 ccagcttatt caccgtgaat gagacctggg gatcgagttg gtccatcgga aaccaggtcg
     2041 tgccgtcata atcattaatg atgaatcctc cgctgacctt gcccgggtca tcggagaatt
     2101 taacgacagc gtcgtcgaat gtgatctccc cgttgcggat ttgaagcagg aggctgtcga
     2161 gctcaagcct ggccttttca aggtcttcgg gtgagggttt tgtttggaga agaatatggc
     2221 gggtattgat ataatcccct tttctttcca ttaactggat gatatggaac ccggctttgg
     2281 tttcaataat atccgatatt tcaccttctt tcagcttaaa tgcagccgct tcgtattccg
     2341 ggtagagttc tccccttcca aagaatccga tctcacctcc tttggcagcc gaggcggggt
     2401 cttccgaata gaggatggcc agcgtggcaa agttttcccc ggccatgacc ctgcgcctga
     2461 ggtcgcggag ccgctctttg atcatcacct tctgttcggc gcttaccggc ggcatcttga
     2521 tgatctgggt catctcgaca cgggcgctga ccaacggcag gctgtcggag ggtatggtgc
     2581 cataaaatgc tttgacttca ttgggtgtga caaagacatt ctttgtgata tcattctgaa
     2641 cctgctcgat cagcatgaaa tccttcacca tctgccggaa ttcctcctta atttccagga
     2701 gagacttttc gtaatactgc tccagttttt cctgtgaacc gaactgggag ataaaatagc
     2761 gcaacctgcg gtcgaggttc tgctccacat cgctgtcgga tacttcaacg ctgtcgagtt
     2821 cggcctggtt cagcaacaaa ttatctctca gcatcttctg caagatctgg catttgaccg
     2881 aagaagccga tccctcgatt tcgccctgca tcctgagttg aagatattgc gattcaagat
     2941 ccgattccag gatcatattc ttgccgacaa tggccacaat ccggtcgacc acaatttcat
     3001 cctgcccgat tccggtcatc ggaagaccgg tcaggatcat tcccaaaaac aatattcccc
     3061 gaaaagcata taggcgcctg gctttcataa tagcatctga tagagattta accacggaat
     3121 gattgtttta ttgttttcag atagattaaa atatcttggc cttatcctct ttaaaggcct
     3181 gatcgataaa ctcctgcctt tttcgttcga taagatcaat ttttcttgca ttcaggataa
     3241 tattcctgat cttatccttt tcaaattcaa ggggggagat actgtcggat gtcttgaaat
     3301 cgtttatcct gacgaaataa ataaaaaggg aatcggttgc ttctgcataa ctgttgcggc
     3361 ggaggaacag ttcctggtta tccacttcca gcggcatttc atcaagcaag tcgttaaatg
     3421 aaacccattc tcctgcaaac cagtaatcgg tggcattctt ttcggcataa tcagcaagcc
     3481 tcatcatact ttcatcttta tccgctttga gcaggttccg aaactcccgt gcatgcctcg
     3541 atcgcttatt gattttgaca aaatcgaact tcacaatgtt tcctttcaat tcaaactggc
     3601 tgcggttttc gatgtaatag gcctcaattt cctgttgggt gatcaccgta tcgagaaact
     3661 ggctgaccag tttttgctcc aggctgaaaa gcagaagcga attcctgtat tcctccagct
     3721 gtttgtcaaa atcctgttcg ttcgatttca gggtttcctt ggctttccgg gtcaggatct
     3781 cgttctggat ccatttggag atatagtttt ctgcaagtgt tatactatcc ctggaaggca
     3841 agccgtcggg tatcttctca gccacttctg agaatctgag ttctttgtcg tttgccctgg
     3901 cgacgacccg gtcgtcgtca tcggccctga aaaggccggt gcaggcaaca agagccggta
     3961 atataagcag gacaattttt ctcacagcgt gctgtttatt tcaatgttga aaggacctcc
     4021 tggttgatgg tgaccgggta cttactgcgc aattcgccaa tccatttctc ctcgaggaat
     4081 gactggtaat cggcagtgac cagtcccctg gcctcctgca aaagcttcgg ttccgggggc
     4141 gctacggcaa tgccgtatac aaaagccgga cccgaaggcg aatcaatcat aggcgacagg
     4201 ccggtcttcc actgaacttt atcgataatc ggggaatctc cgcgcgaata tttttctgtt
     4261 tcagccagga tattcagcgt agtatccgaa ttgtaacgtt caaggatctc ttccagtggt
     4321 ttcccctcgg tgatgaaatc tctcagctct tcgatattga catccccggg gttcaaaaca
     4381 gtgacgacgg tggcatggaa acgctcatcc cacatgtact tatctttatt ggcgccataa
     4441 taggcagcca gcccggcagt atctttcacc gctttcgacc agacatttct ctccatcagg
     4501 tcgaaaagca gtataccgtc gtggtattca cggatcaaaa gcctgaaatc gggatacttt
     4561 tgctcaagac ggctgtcttc atatgccatg catttttctt tcacgaagct tttatatgtg
     4621 tcattgaaga atccccggat gctggtaacg ctcttggttt gtttggaagc aaggtaagtt
     4681 gccagatcct gctgggaata cttgagtttg tcgatggaaa acaccggttt cttcatcccg
     4741 gtgccgatca tcgcgcattt catgaagg
//
LOCUS       DAOQXS010000200         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18260_length_4749_cov_3.475075, whole genome shotgun sequence.
ACCESSION   DAOQXS010000200 DAOQXS010000000
VERSION     DAOQXS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18260_length_4749_cov_3.475075"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKM34_06635"
     CDS             complement(<1..843)
                     /locus_tag="PKM34_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ83302.1"
                     /translation="MAIIDLVRWAPQGDQTIYAFRFPETNLSTYTQLIVQESQEAILF
                     SKGQIVGKFGPGKHTLSTENLPILRNLFGLPFGGKNPFTAEIWFINKLQPYNIDWQID
                     RMDIHDADYNTGIPLVAKGRYGLRINDSERFLVKIVGPKESFDEFDLTDQFSGEFHTK
                     TKSTIMQFMLNNGIGLKKISAFLDNISEHLRDIMLPFWENLGFELTRFYVTSIEVDGT
                     TEVGKRILDALSRQSAQAIGGYTWQQSQAFEVAKDAVDGLSNSSNGLLGAVIASNMMG
                     GLGGG"
     gene            1116..2552
                     /locus_tag="PKM34_06640"
     CDS             1116..2552
                     /locus_tag="PKM34_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNQ83303.1"
                     /translation="MVFSSTLFLLYFLPVFLGIYFLLPNKLRNLWLLVASVLFYSWGA
                     PDFIFIVLGSILADFYLVKYIHRAGLKRKRWLTAISIVMNVGLLAYFKYANFFVDNLN
                     SLLTGLGMTQLHWTAIALPIGISFFTFQKITYTVDVYRKVHAPLKKITDYAMYILMFP
                     HLIAGPIVRFNLVADQIEDRRYNETIGNRVNGLFRFVIGLSKKVLIANVLGAEADKIF
                     AMDPSTFGAAQAWTGIIAYAFQIYFDFAGYSDMAIGLALMIGFNFPENFNNPYISQNI
                     TEFWRRWHMTLGRFMREYLYIPLGGNRVGTARLYLNLWIVFFLSGLWHGAAWNFVAWG
                     AFHGLFLVADRVFLIKWLTKAGKYPAVAITFFISLIGWVLFRSETLGYAMRFLRSMFT
                     FGSDFGGVTLNNKFIVILVIAVIFSFIGIFKGNEAWQERILRGRGDGEMGRFGDLGGR
                     LIFSLVFLLLCLAAISSSGFNPFIYFRF"
     gene            2552..4321
                     /locus_tag="PKM34_06645"
     CDS             2552..4321
                     /locus_tag="PKM34_06645"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83304.1"
                     /translation="MKKALPHILFAVILLIALLPAVQMRWSLIEEKPLNGAFELAEKP
                     VLSYENWMSGDFQMQTEKYLKDHSGFRNFLIRLQNQLDFSLFKQANAEGATVGKKGQL
                     FEYDYIRSWLALDYPGDSFLEKKLQRARFVQDYLKREKGIDLVVVFEPGKASYYPEYL
                     PSSYKKQKKGPSTYDYFVKKAKETGIDYIDLQQYFLELKDTSRYPLFPAYGTHWSVYG
                     MKFAADSLLRLIGNRRNLDLTDVRLTGIETSTRPRDTDDDVLKTMNLLCPMQGEELAY
                     PLLAFDTLNPGEKPMVLVVADSYYWNIFNTRIPKYVFANEAFWYFNALVYPDHYNRPT
                     FTKDLDLKAEIEKQDIIFLMITERFVHKFDWRFTDQLYELYAPQGLSDPVYDKINNIM
                     TVEEWFEKMITKAGYKHATLEEILIDEGKYLYHMSDTAGFMIRYGEEYFRKMISGDRK
                     WMGHIEEKAARRGIPVEDMLVEDALFVFKEKHPELYKLNRSLESVRKHILANPDTLAL
                     LREEARNYHFDPDLYLQHRAWEIVHRDEVAKTERAIRQYAPWLEDVARKALENGICVD
                     EMIRLDAEYMWKERLRSVGFKEK"
     gene            4369..>4749
                     /locus_tag="PKM34_06650"
     CDS             4369..>4749
                     /locus_tag="PKM34_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNQ83305.1"
                     /translation="MPTTADFRNGLCIDFNNDLWSIVEFQHVKPGKGGAFVRTKLKNL
                     RDGRVLENTFIAGVKINIVRIERRPHQYLYNDGESYYFMNADTFEQVFVEKKLLEAAD
                     LLKEGVTVDLIFHSETETVLQCEMP"
BASE COUNT         1240 a         1157 c         1113 g         1239 t
ORIGIN      
        1 accaccgcca aggcctccca tcatgttgga tgcgatgact gctcccagca atccgttact
       61 gctgtttgag agtccgtcaa cggcgtcttt ggccacttca aatgcctgcg attgctgcca
      121 ggtatatccg ccaatggcct gggcactctg ccggcttaaa gcatccagga ttctctttcc
      181 cacttcggtt gttccgtcga cttcaatgct ggtaacataa aaacgggtca gttcaaagcc
      241 aagattttcc cagaaaggaa gcatgatgtc acgcaagtgc tctgaaatat tatctagaaa
      301 ggcagaaatc ttcttcagtc caataccatt attcagcata aattgcatta ttgtggattt
      361 tgtctttgtg tgaaattcac cggaaaactg gtcggtcagg tcaaattcat cgaatgactc
      421 tttcgggcct acaatcttca ccaggaaccg ctccgaatca ttaattctca gtccatatct
      481 tccttttgcc acaagaggaa ttccggtgtt ataatcggca tcatgaatat ccatacggtc
      541 aatctgccaa tcgatattat acggttgtaa cttgttgatg aaccatattt ctgccgtgaa
      601 aggatttttc cctccaaaag gtaaaccgaa taaatttctc agaatcggaa ggttttccgt
      661 gcttagggta tgtttacctg gtccaaactt acctacaatc tgcccctttg agaacaaaat
      721 agcctcttgc gattcctgga cgatcaattg agtataagta ctcagatttg tttcgggaaa
      781 ccggaatgcg taaatcgttt gatctccctg tggtgcccat cttactaaat ctataattgc
      841 cataaataat accttgttta ttaatgaatt aaataattag atcacgataa taatatttta
      901 aaccgaaata gaataagaaa ctatgacaag cgataaaacc ataataatgg actcttttga
      961 tatggcggat taaagttatc aggaaaattg cttaattcca atgtttataa aaactttttt
     1021 ttgagatgtc aagattttga ttcgatatta ccgaaaaata taatattgca aaaaattcga
     1081 ttgcaatcag atgtaaatct ttccaaacag agtaaatggt tttcagcagc accctattcc
     1141 ttctttattt cctgccggtt ttcctgggca tctacttctt gctgcccaac aaactgagga
     1201 acctgtggct gctcgtcgcc agcgtcctct tttattcctg gggcgccccc gattttatct
     1261 ttatcgtcct cggatcgatc ctggccgatt tctacctggt caaatatatc caccgggccg
     1321 gcctgaaacg gaaaagatgg ctcaccgcca tctccatcgt gatgaatgtc gggctgctgg
     1381 cttatttcaa atacgccaat ttctttgtcg ataacctgaa cagtctgctt acaggactgg
     1441 gaatgacgca gttgcactgg accgctatcg ccctgccaat cggaatttct ttcttcactt
     1501 tccagaaaat aacctacacg gtggatgttt accggaaggt ccacgcgccg ctgaaaaaga
     1561 tcaccgatta cgctatgtac atcctgatgt tcccccacct gatcgcgggg ccgatcgtca
     1621 ggttcaacct ggtcgccgac cagatcgaag accggcgtta taatgaaacg atcgggaacc
     1681 gggtcaacgg gctgttccgc tttgtgatcg ggctgtcgaa gaaggtgctg atagccaatg
     1741 tcctgggcgc tgaagccgac aaaatctttg ccatggaccc ctccacgttc ggcgcggcac
     1801 aggcctggac cggcatcatc gcctatgctt tccagatcta tttcgacttt gccggctact
     1861 ccgatatggc gatcggcctg gccctcatga tcggtttcaa tttccccgaa aatttcaaca
     1921 acccgtatat ttcacagaat atcaccgagt tctggcgccg ctggcatatg acgctcggcc
     1981 ggtttatgcg ggaatacctg tatatcccgc tgggcggcaa tcgtgtcgga actgcccgcc
     2041 tctacctgaa cctctggatc gtattcttcc tttccggcct ctggcacgga gccgcgtgga
     2101 atttcgtggc ctggggcgct tttcacggcc tcttcctggt agccgaccgg gtcttcctga
     2161 tcaaatggct cacgaaagca ggcaaatacc ccgctgtagc catcactttc ttcatctccc
     2221 tgatcggatg ggtcctgttc agatcagaaa ccctcggtta tgccatgaga ttcctgcggt
     2281 cgatgttcac cttcggcagc gatttcggag gtgtaacgct gaacaacaag ttcatcgtga
     2341 tccttgtcat tgctgttatc ttttcgttca tcggtatttt caaaggaaat gaagcctggc
     2401 aggagcggat cttgagagga cggggagatg gggagatggg gagatttgga gatttggggg
     2461 gtagactgat ctttagtttg gtattcctgt tattgtgcct ggcggcgatc agttcttcgg
     2521 gttttaatcc attcatttat ttcagattct gatgaagaag gcattacccc atatcctttt
     2581 tgcggtgatc ctcctgatcg ctttgctgcc ggcggtgcag atgagatgga gtctcataga
     2641 ggaaaaaccg ctcaacggcg ccttcgaact ggccgaaaaa cctgtcctgt cttatgaaaa
     2701 ctggatgtcg ggcgatttcc agatgcaaac tgaaaaatac ctgaaagacc actccggctt
     2761 ccggaacttt ttaataagat tgcaaaacca gcttgacttc tccctgttca aacaagccaa
     2821 tgcggaaggg gccactgtcg gcaaaaaagg gcagctcttc gaatatgatt acatccgttc
     2881 ctggctggcg ctggattatc ccggcgattc gttcctggaa aagaagctgc agcgggcccg
     2941 ttttgtccag gattacctga aaagggaaaa agggatcgac ctggtcgtag tcttcgaacc
     3001 gggcaaagct tcctactatc ccgaatatct tccatcatct tacaaaaaac aaaaaaaagg
     3061 cccctctacc tacgattact ttgtgaaaaa agcaaaggaa acggggattg attatatcga
     3121 cctgcagcag tatttcctgg aactgaaaga taccagccgg tacccgctat ttcccgcata
     3181 cggcacacac tggagcgtct atgggatgaa atttgccgcc gacagccttc tccgtctgat
     3241 cgggaaccgg agaaacctgg acctgaccga tgtcaggtta accggtatcg aaacctcgac
     3301 cagaccaagg gatacagacg atgatgtact gaaaacgatg aacctcctct gcccgatgca
     3361 gggcgaagaa ctggcctatc ccctcctggc gttcgatacg ctgaatcccg gcgaaaaacc
     3421 gatggtgctg gtggtggccg acagttatta ctggaacatc ttcaataccc ggatcccgaa
     3481 gtatgttttt gccaacgaag cgttctggta cttcaacgcc ctggtttacc ccgatcatta
     3541 caacaggcca acctttacga aagatctgga cctcaaagcg gaaattgaaa agcaggacat
     3601 catcttcctg atgatcaccg agaggttcgt gcacaaattc gactggcggt tcaccgacca
     3661 gctttatgag ctttatgcac cgcaagggct ctccgatccg gtgtacgaca agatcaacaa
     3721 cattatgacg gttgaggagt ggtttgaaaa aatgatcacc aaagcgggat ataagcatgc
     3781 gacactggaa gagatcctga tcgatgaagg gaaatacctt tatcatatgt cggatacggc
     3841 cggtttcatg atccggtatg gcgaagaata tttccgcaag atgatctccg gtgaccggaa
     3901 atggatgggg catatcgaag aaaaggcggc ccggcggggc attccggtgg aggatatgct
     3961 ggtagaggat gctttgtttg tttttaaaga aaagcatccg gaattgtata agctgaaccg
     4021 cagccttgaa tccgtgagaa aacacatttt agccaatccc gacaccctgg ccttactacg
     4081 cgaagaagcc cggaattacc attttgatcc cgatctctat cttcagcaca gggcctggga
     4141 aatcgtccac cgggatgaag tggccaaaac ggaaagggcc atccggcaat atgccccctg
     4201 gctggaggac gtggcacgca aggcactgga aaacgggatc tgtgtcgacg aaatgatccg
     4261 gctggatgcc gaatatatgt ggaaagagcg attacgatcg gttggtttta aggaaaaata
     4321 gtttactttt gcacaaaatt tcaaaatcct ttactaaatc cagattatat gcctactacc
     4381 gctgatttca ggaatggctt gtgcatcgat ttcaataatg acctctggtc gatcgttgag
     4441 tttcaacatg tgaaacccgg caagggaggc gcgtttgtga gaacgaagct gaaaaacctc
     4501 cgcgacggca gggtgctcga aaacaccttt atcgccggtg taaagatcaa catcgtcagg
     4561 atcgaaagac gtcctcacca gtatctctac aatgacggcg aatcctacta tttcatgaat
     4621 gccgacacct tcgagcaggt gtttgtcgag aagaaactgc tggaagccgc cgatctcctg
     4681 aaagaagggg taacggtcga tttgatcttc cattccgaaa cggaaacggt tttgcaatgt
     4741 gaaatgccc
//
LOCUS       DAOQXS010000201         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18277_length_4746_cov_3.049243, whole genome shotgun sequence.
ACCESSION   DAOQXS010000201 DAOQXS010000000
VERSION     DAOQXS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18277_length_4746_cov_3.049243"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /locus_tag="PKM34_06655"
     CDS             complement(<1..2198)
                     /locus_tag="PKM34_06655"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ83306.1"
                     /translation="MKNIIRYTLLLVFAILAISQAAGQKKTFIRTADEAFEDERFSVA
                     VEKYQKAYTKLKKNPVERDRISFRMAECYRMMGELKRADIQYKRLIKNGYDSKEPIIL
                     LHYADYQKADGNLTEAKEYYTKYIEKVPDDPRGKYGLEACEKIPNWQEYESKYQISEE
                     KGLNSRESDFAPTYASDNFNSLIFTSTREGSKGKGIDEWTNQGFSCLFQSRKDVKDAW
                     STPVLVDDAEEGGINTEANEGAPMMNKNYTRLYFTRCPTDEGRKNGCQIYTSNRTGRT
                     WSTPDLIPLGLDSTEAVGHPTVSDDEMILYFSSNRPGGMGGKDIWVAVRETPGGPFGR
                     PYNLGPRINTPGDELFPYLRYDTLLYFASDGHPGLGGLDIFYTFPDTARMWSTPVNIG
                     IPINSSSDDFAIIFQPKGEQGFFSSNRSGRKSREDIYSFVIPAVAFTLSGTVKDDRTL
                     QVIPGARVDIVGSDGTSMTARTSETGVYMFGKSQIMPNTTYEMTVSKDNYFNASGRET
                     TVGYEKSKDLVRNFVLEPIPEEPVVLPEILYDLAKWDLKPQYQDSLQGLITTLDENPT
                     LKIELASHTDARDTEERNDILSQRRAQSVVDYLIERGIDPDRLVAKGYGERVPRLLLK
                     DITRDGFTFKEGTVLTEKYIDSLATTPEKEAAHQLNRRTEFRVISKDFVPKPKNVELG
                     KTVEILINPDDNILKYTPAPKTGNITAACIINGYTAQFTFDAKLIPQISV"
     gene            complement(2260..2616)
                     /locus_tag="PKM34_06660"
     CDS             complement(2260..2616)
                     /locus_tag="PKM34_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNQ83307.1"
                     /translation="MPQMLLFFDISGGELMVILFVAFLVFGPSKIPEVARKIGRGMNE
                     IRRASDEIKREINKESRKLEHEVNGEGSIYRDIKKSTEEIRQDFEDAAKPSDEVGKEP
                     PEENNHIGSRDSGDFR"
     gene            complement(2716..>4746)
                     /gene="secD"
                     /locus_tag="PKM34_06665"
     CDS             complement(2716..>4746)
                     /gene="secD"
                     /locus_tag="PKM34_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ83308.1"
                     /translation="QSATVGFAAIKDTAKVNDMLRKVNSVFPRNLKLVWTVKPRDANS
                     DVLELVALKVTSRDGTPALGGEVIVDARQDYDQLGNVEVSLNMNAEGARTWKRLTGEN
                     IDKQIAIVLDNHVYSFPTVRGEIPNGMSSISGGGMTLEEAQDLSNILKAGKLPASARI
                     VEEAVVGPSLGQEAINAGLWSFVIAFLLVLLYMVFFYARAGWIADLALIVNMFFILGV
                     LASLGAVLTLPGIAGIVLTLGMAVDANVIIYERIKEEMRAGKGIRLAISDGYKAAYSA
                     IIDGNVTTLLTAIVLYIFGTGPVQGFATTLIIGILSSLFTAIFISRIIFTTLLDKNKT
                     ISFEMPTTRGFLQNTKIDFVSLRKYAYIFSITVSVIGLISMFTRGFDQGIDFAGGRTY
                     VIRFDQPVSTIEVRNALEGQFEGNAPEVKTFGPASQVKVTTKYLIDNKGPEVDSIIQN
                     KLFTGLKPFYTNPSFAYTDFTTDATEAGKLLGIRSSQKVDPTISDEILWRSIRAIAIA
                     LIIIFVYIALRFKKWQYGLGGLISLFHDALITLGIFSLLYSIMPFSMELDQALIAAIL
                     TIIGYSINDTVIIFDRIREYVTLHPKRDYKTNVNEAINSTLARTVNTAGTTLIVLIAI
                     FIFGGEVIRGFTFALLFGVAFGTYSSVFVATPIAYDLMMKKLKKSDKAKAIK"
BASE COUNT         1099 a         1172 c         1163 g         1312 t
ORIGIN      
        1 ttgacagaaa tctgcgggat cagcttcgca tcgaatgtga attgtgccgt gtacccgttg
       61 ataatgcagg cagccgtgat gttcccggtt ttgggcgccg gggtgtattt taagatgtta
      121 tcgtccggat tgatcaggat ctcaacggtt ttgcccagtt caacattttt cggtttgggg
      181 acaaaatcct tgctgatcac cctgaactcg gtccgcctgt ttaactggtg agcggcttct
      241 ttttcgggcg tcgttgccag cgaatcaatg tacttttctg tgagaacggt tccttccttg
      301 aaggtgaatc cgtcacgggt gatgtctttc agcaagagcc tcgggaccct ttccccgtag
      361 cctttagcca ccagtcggtc agggtcaatg cctctttcga tcaggtaatc gaccaccgac
      421 tgggcacggc gttgcgacag gatgtcattt ctttcttcgg tgtcgcgggc atcggtatgg
      481 gaggccagct cgatcttcag tgtcggattc tcatccagtg tggtgatcag cccctgcagc
      541 gaatcctggt attgcggttt caaatcccat ttagccaggt catacaaaat ctccggcagc
      601 accaccggtt cttcgggaat cggttccagg acgaaattcc gcaccaggtc tttgcttttc
      661 tcataaccga cggttgtttc ccgcccgctg gcattgaaat agttatcttt tgatacggtc
      721 atctcatagg tggtattcgg catgatctgc gacttgccaa acatataaac tccggtttcg
      781 gaggttcttg cggtcatcga cgtcccatcc gaaccgacaa tatccacacg cgcccccgga
      841 atgacctgca aagtccggtc atccttgaca gttcccgata aagtgaaagc cactgcggga
      901 ataacaaagg agtaaatatc ttccctggat tttcttccac tccggtttga tgagaaaaat
      961 ccctgttcgc ctttcggctg aaaaattata gcaaaatcgt cggaggaaga attgatcggg
     1021 attccgatat tgacaggtgt tgaccacatc ctggcggtat cggggaaggt atagaatata
     1081 tcaagtcctc ccagtccggg gtggccgtcg gaggcaaaat aaagcagcgt atcgtatctc
     1141 aggtaaggga atagttcatc gccgggggta ttgatgcggg gccccaggtt gtacggacgg
     1201 ccgaacgggc ctcccggtgt ttcccttact gctacccaga tgtcttttcc tcccattccg
     1261 cccggccggt tcgatgagaa gtaaaggatc atctcgtcgt cgctgaccgt gggatgcccg
     1321 accgcttccg ttgaatcaag ccccagcgga atcaggtccg gcgtgctcca ggtacggccg
     1381 gtccggtttg atgtgtagat ctggcatccg ttcttgcggc cttcgtcggt ggggcagcgg
     1441 gtaaaataga gccgggtgta gttcttgttc atcatgggcg ctccctcatt ggcctccgta
     1501 tttatgccgc cctcttcggc atcatccacc agaaccggtg tgctccaggc atccttgaca
     1561 tctttcctcg actggaacag gcaactgaat ccctggttgg tccactcgtc aatgcctttt
     1621 cctttgcttc cttcgcgggt ggaggtaaag atcaggctgt tgaaattgtc gctggcataa
     1681 gtcggcgcaa aatctgattc ccgggaattt aatcctttct cttccgaaat ctggtatttc
     1741 gactcgtatt cctgccagtt cgggatcttt tcgcaggcct ccaggccata ttttccccgt
     1801 ggatcatcag ggactttctc aatatatttg gtataatact cttttgcttc agtcaggttt
     1861 ccgtcagctt tctggtaatc ggcataatga agcaggatga tgggttcttt gctgtcatag
     1921 ccattcttta tcaaccgttt gtattggata tcggctcttt tgagttctcc catcatccgg
     1981 tagcattccg ccatacgaaa ggagatccgg tcacgctcca ccgggttttt tttaagcttg
     2041 gtataggctt tctggtattt ttctactgca accgaaaacc gttcatcttc aaatgcctcg
     2101 tctgccgtgc ggataaaggt cttcttctgt ccggcagcct gactgatggc cagtatggca
     2161 aataccagga gtaatgtgta tcggataata tttttcatgc tacaacagca attcatacat
     2221 tttttattta acggagaagc atcaaaatta tttgaatgat cacctgaagt cgccggaatc
     2281 ccgtgagcct atatgattat tttcttccgg tggttctttc ccgacttcat ctgacggttt
     2341 tgccgcatcc tcaaaatcct gtctgatctc ttccgtagac ttctttatat ccctgtaaat
     2401 cgaaccttcg ccgttcactt cgtgctccag tttccggctt tctttattga tctcccgttt
     2461 gatttcatcc gatgcgcgcc ggatttcatt catccccctg ccgatcttcc gggcgacttc
     2521 gggtatctta ctgggcccaa aaaccaggaa agctacgaac aggatgacca tgagttcacc
     2581 tccgctgata tcaaaaaaca gtagcatctg tggcataggg ccgtaattga taagaacaca
     2641 aaagtaataa aaaaggctgg ttgtccggaa attttacctc cttgtcaacc agcccggttt
     2701 tatgtaatga tttatctatt tgatggcttt tgccttgtcg cttttcttta atttcttcat
     2761 catcaggtcg tatgcaatgg gtgtagcgac aaataccgag gaataagtac cgaacgcaac
     2821 accgaagaga agggcaaagg taaatccgcg gatgacctca ccgccaaaaa taaagatggc
     2881 gatcagtacg atcagggttg tgccggctgt gttgaccgta cgggccagtg tgctgttaat
     2941 agcttcattg acatttgttt tgtagtcccg cttcggatgc agggtgacat attccctgat
     3001 cctgtcgaag atgatcaccg tatcgttgat ggaataaccg atgatcgtca ggatggcggc
     3061 gatcagcgcc tggtctaatt ccatgctgaa cggcatgatg gaataaagca gcgaaaatat
     3121 tcccaaagta atcaaggcat catggaacaa tgaaatcagg cctccgagtc cgtattgcca
     3181 tttcttgaac cgcaacgcta tatatacaaa aatgattatc aaagcaatag caattgcccg
     3241 gatagaacgc cacaaaattt catccgatat tgttggatca actttttgtg agcttctgat
     3301 tcccagcaat ttaccggctt cagttgcgtc tgtcgtaaag tctgtgtagg caaacgacgg
     3361 gttggtataa aatggtttga gaccggtaaa gagcttgttc tggattatcg aatcgacctc
     3421 aggcccttta ttatcaatca agtatttagt ggtcacttta acctgtgatg caggtccaaa
     3481 tgttttaact tcgggtgcat tgccttcgaa ctgtccttca agggcattcc tgacctcgat
     3541 ggtgctgacc ggctgatcga agcgaatgac ataggtcctg ccgcccgcga aatcgatgcc
     3601 ttgatcaaat cccctggtaa acattgagat taggccaata acagagactg tgattgaaaa
     3661 aatgtaagca tatttccgta aactgacaaa gtcgatcttt gtgttttgca gaaatccccg
     3721 ggtcgtgggc atctcaaatg atatggtctt gtttttatca agaagtgtag tgaagatgat
     3781 cctggaaatg aaaatagctg tgaacaggga gctgaggata ccgatgatca gtgtagtggc
     3841 aaaaccctga acaggacccg taccgaatat atagagtacg atagctgtaa gcagagtcgt
     3901 tacgttcccg tcgataatag cggaataggc ggctttatat ccgtcggaga ttgccagcct
     3961 gatccctttg ccggccctca tttcctcctt gattcgttcg tagataatta cgttggcatc
     4021 cacggccata ccgagggtta agacgatccc ggcaataccg ggaagggtca gtaccgcccc
     4081 cagggaggct aaaactccga ggatgaagaa catattgacg atcagggcca ggtcggcgat
     4141 ccatccggcg cgggcataaa agaataccat ataaagcagc accagcagga atgcgatgac
     4201 gaatgaccat aaacccgcat tgattgcttc ctgacccagt gaagggccta cgaccgcctc
     4261 ttcgacgatg cgtgccgaag cggggagctt accggctttg aggatattgg agagatcctg
     4321 tgcttcttcc agggtcattc cgcctccact gatggaagac ataccgttgg ggatttcacc
     4381 tcttactgtc gggaatgaat aaacgtggtt gtcgagcact atagcgatct gtttatctat
     4441 gttctcaccg gtgaggcgtt tccatgtccg ggcaccttca gcattcatat tcaggctgac
     4501 ctcaacattg ccgagctggt cgtaatcctg gcgggcatct acgatcactt caccgccaag
     4561 ggccggggtg ccgtcgcgtg aggtcacctt aagcgctacc agttcgagca catcggagtt
     4621 ggcatctctg ggcttaaccg tccataccag cttgaggttc cgggggaaga cgctattgac
     4681 tttcctgagc atatcattaa ctttcgccgt atccttaatg gcggcaaatc ctactgtcgc
     4741 gctttg
//
LOCUS       DAOQXS010000202         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18309_length_4740_cov_4.745784, whole genome shotgun sequence.
ACCESSION   DAOQXS010000202 DAOQXS010000000
VERSION     DAOQXS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18309_length_4740_cov_4.745784"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PKM34_06670"
     CDS             complement(<1..431)
                     /locus_tag="PKM34_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83309.1"
                     /translation="MIYGKKITVVLPAYNAEKTLEQTYREIPFDIVDEVILVDDKSRD
                     NTVEVAKRLGIHHIIEHQENKGYGGNQKSCYDKALEIGSDIVVMLHPDYQYTPKLIHS
                     MCYVIANDVYPVVLGSRILGKGALRGGMPLYKYISNRFLTLA"
     gene            complement(446..2230)
                     /locus_tag="PKM34_06675"
     CDS             complement(446..2230)
                     /locus_tag="PKM34_06675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ83310.1"
                     /translation="MIGQKYNNNLQYLLIVVAGALLFLPFLGNVALFDWDEVNFAEIA
                     REMIVTGDYLNVQINYQPFWEKPPLFIWMQALSMKLFGINEFAARFPNAICGITTLLV
                     IFAIGKKIHNEKMGLTWVLVYAGSILPFFYFKSGIIDPWFNLFIFLGIYFATISIDRS
                     RVQLFNGSIVNEPDISSYNPTVLQSNSHKFLHSPGLLVFLSGAAIGLAILTKGPVALL
                     IFGLVAIVFPFSFILTNIKKSTTSQNQISKFPNFQIITKSLSLFAIGLLLTGGSWFLL
                     MIANGHLDVVIDFIVYQVRLFSTKDAGHGGFPGYHVVILMLGVFPASAFFFGGLKAVP
                     EENFLQKNWRRVMIILLATVVVLFEIVQTKIVHYSSLAYFPLTYLAALALYRASLSER
                     AFSRSSRIILWTLAILWMTVIAGFTLVGLNKVWLMNSGLIKDPFAVANLAADIRWTGF
                     ETLVALVLIPGIAGFFILKKPFHKMIALFSTSLVFTFLTMTIFTGKIEGYVQRAPIEF
                     YEKHSSEECYINTLGFKSYAHLFYGKKMPPVNPISYDKEWLLNGKIDKNAYFVYKITK
                     KEEYSRLYPQLTVLYEKNGFIFAARYPE"
     gene            2395..2829
                     /locus_tag="PKM34_06680"
     CDS             2395..2829
                     /locus_tag="PKM34_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ83311.1"
                     /translation="MNRKIFLTKPGIYCEKMGLIRKNNMNRIELEKRLISFASAIITL
                     CKECRFDDYTFHLAKQIVRSASSAALNYGEAQSAESRADFTHKLSLVLKELRETQVNI
                     TIMSNSVICNNPLRIVDLLKECQDLIAIFQKSINTLNNKNNR"
     gene            complement(2894..4433)
                     /locus_tag="PKM34_06685"
                     /pseudo
     CDS             complement(2894..4433)
                     /locus_tag="PKM34_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
BASE COUNT         1246 a         1084 c         1176 g         1234 t
ORIGIN      
        1 gccagcgtaa ggaatcggtt ggatatgtac ttgtaaagcg gcatcccgcc cctgagagca
       61 cctttcccga gaatacgcga tcccaggaca acggggtaaa cgtcgttagc aatgacgtag
      121 cacatgctgt ggataagctt tggcgtgtac tggtaatcgg gatgaagcat tacgacgatg
      181 tcggaaccga tctcgagcgc tttatcgtag cagctcttct ggtttcctcc gtatcctttg
      241 ttttcctggt gctcgatgat atgatggatc ccgagccgtt ttgccacctc cacggtgtta
      301 tcccggcttt tatcatcgac caggatcacc tcatccacta tatcgaacgg tatctcccgg
      361 taggtttgtt caagggtttt ttccgcattg tatgccggta gtacgacagt tatcttcttg
      421 ccataaatca tattcctgca ttttattatt caggatatcg cgccgcaaag ataaaaccgt
      481 tcttctcgta taaaacggtc agttgaggat aaagacggga atattcttct ttcttcgtta
      541 ttttatatac aaaataggcg ttcttatcaa tctttccgtt gagcagccat tctttatcat
      601 aagaaatcgg attgaccggc ggcattttct ttccataaaa aaggtgggca taacttttaa
      661 aaccgagggt attgatataa cactcctcac tactgtgttt ttcataaaat tcgatgggag
      721 cacgctgaac atatccttct attttaccgg tgaaaatggt catggtgagg aaggtaaaga
      781 ccagggaggt gctgaaaagc gcaatcattt tatgaaaagg ttttttcagg atgaagaagc
      841 cggcaattcc cgggatcagc accagggcaa ccagagtctc gaagcctgtc caccggatgt
      901 cggccgccag gttggcgacg gcaaaggggt ctttgatcag gccactgttc atcagccaca
      961 ctttgttcag accaaccagt gtaaatcctg cgattaccgt catccagagg atggccaggg
     1021 tccacaggat gatgcgggaa ctgcgggaaa atgctctctc tgaaaggctg gcccggtaaa
     1081 gggccagggc agccaggtag gtcagcggga aataggccag ggaactgtaa tgaacgatct
     1141 ttgtctgaac aatctcgaaa agaaccacca cggttgcgag aaggatgatc atcacccggc
     1201 gccagttttt ttgcaggaaa ttctcttccg ggacggcctt tagtcctccg aaaaagaatg
     1261 ccgatgccgg gaagacaccc agcatcagga taaccacgtg gtatcccggg aaaccgccgt
     1321 ggcctgcgtc tttggtgctg aacaggcgaa cctggtatac gatgaagtcg atcaccacat
     1381 cgaggtggcc gttggctatc atgagaagga accaggagcc gccggtgagg aggagaccga
     1441 tggcgaaaag agagagggat tttgtgataa tttggaaatt tggaaatttg gagatttgat
     1501 tctgtgaagt tgtggatttt ttgatgttgg tcaggatgaa agagaacggg aagactatgg
     1561 caaccagtcc gaagatcaga agggcgacgg ggcctttggt gaggatagcc agaccaatgg
     1621 cggcaccgga aagaaagaca aggagaccag gggaatgtag gaatttatga ctgttggact
     1681 gtaagacggt aggattgtaa gacgagatgt cgggttcatt aacaatcgaa ccattgaaca
     1741 attgaactct tgatctgtcg atggaaatgg ttgcaaaata tatgcccagg aaaataaaca
     1801 ggttgaacca gggatcgatg atgcccgatt tgaaatagaa gaacggaagg atggaaccgg
     1861 cgtaaaccaa cacccaggtg aggcccattt tttcattatg gatttttttt ccgatggcga
     1921 agatgaccag cagcgttgtg atgccgcaaa tagcattggg gaaacgggcg gcgaattcat
     1981 tgatgccaaa aagcttcatc gacaacgcct gcatccagat gaacagcggc ggcttttccc
     2041 agaatggctg ataattgatc tgcacgttga ggtaatcgcc cgtaacgatc atctcccggg
     2101 cgatctcggc aaaattgacc tcatcccagt cgaacagggc gacgttgccc aggaaaggca
     2161 ggaaaaggag cgccccggcg actactatca gtaagtactg aaggttgttg ttgtatttct
     2221 gaccgatcaa gatttcaatt atttgaatgt ggtaaaaata ataaatgatt tacagtttac
     2281 ggattacagt ttacaatcta taatctgaat tttgcattga agattgtact ctgcagaatg
     2341 taaaaagtaa tctctaaatt gtaattcttg acaattgtaa ctaccacaag attaatgaat
     2401 agaaaaattt ttttaactaa accgggtatt tattgtgaga aaatgggatt aattagaaaa
     2461 aacaatatga accggattga actggaaaag cgactaatta gctttgcatc ggcaattatt
     2521 acgctttgta aggaatgtcg ttttgatgat tatacttttc atcttgctaa acagattgta
     2581 agatctgcca gctcggctgc attaaattat ggtgaagcac agagtgcgga atcacgtgcg
     2641 gattttactc ataaacttag ccttgtactc aaagaactga gagaaactca agtgaatata
     2701 acaataatga gtaactctgt aatttgtaat aatcctctta gaatagtaga tcttttaaaa
     2761 gaatgtcagg atctcattgc tatttttcaa aaatccatta acacactgaa taataagaat
     2821 aaccgatgat catattccat aatccacttc aaagattgta aactgtaaat tgtaaatcgt
     2881 aaatcgaagt tcctcactcg tactctcccc acgccttgat cccgatctcc tcgatcccga
     2941 tcctgcagaa cgagtagata aatgcgctgg ccagccgggc attggtgatg agcgggatgt
     3001 taatcgacgg cactgcggcg gatgcggtaa tcgttgtcaa gctcgctttt gctcaggtcc
     3061 ttggggatgt ttatcaccag gtcgatcttc ttccggcgca ggtattccat cgtgttcggg
     3121 gttttatcct cgcccggcca gtgcagcatg atcgaattaa tcccgttttt gatgaagaaa
     3181 tcgtgggtgc cgccggtggc ataaatggtc agacctttct cctgcagcat ccgggcgctg
     3241 gtgagcaatt cctgcttcga ccgcgacggg ccgctggaat acagcacagc tttctccggg
     3301 atcgtatagc cgaccgacaa catcgatttc agaatggctt cgtagtaatt gtcgccgatg
     3361 cagccgacct cgccggtgga aaccatttcg acgcccagca caggatcggc tttttgcaat
     3421 cgggagaatg agaactgcga ggctttgacg ccgacataat cgagatcgaa caacgattta
     3481 tccggtttgg ccaccgtttt tcccagcatg attttcgttg cggtttcgat aaaattggtt
     3541 ttcagtactt ttgagatgaa cgggaagctg cgagaggctc tgagattgca ctcgatcacc
     3601 atcacatcgt tgtctttggc caggaactgg atgttgaacg gccctgaaat tttcaggtgt
     3661 tgcgcgatct gccggctgat ccgcttgatc cgcctcaccg tttcgacata gattttctgt
     3721 ggcggaaaca gcatggtggc atctcccgag tgaacgccgg cgaactcaac atgttccgag
     3781 atcgcatagc aaatgatctc cccgttcgcg gctacggcat ccatctcgat ctcctttgcg
     3841 tcttcaatga acctggaaac caccacggga tactgtttcg acacctccgc cgccatctcc
     3901 aggaaatgtt cgagctcgct gcggttcgac acaatgttca tggccgcacc cgacaaaaca
     3961 tacgacggcc ttacaatgac cgggaaaccg acctcatcgg taaaccggta tacctcgctt
     4021 acggaagatg actcgatcca ccggggctgg tcgatgccaa gttcgtcaag gatagccgaa
     4081 aatttgtgcc ggttctcagc tttgtcgatc gactgcggcg aggtgccgag aatcgccacc
     4141 tcctgctccc agagccgaat cgccaggttg ttcggaattt gcccgccggt tgaaacgatc
     4201 acgcccaggg ggttttcaag atcgaggatg tccatcaccc tttcaaaggt aagttcctcg
     4261 aaatagagcc ggtcgcacag gtcataatcg gtgctgacag tctcggggtt gtagttgatc
     4321 atcaccgaac ggtatccttc cttattaaca cattccagcg cattcacccc gcaccagtcg
     4381 aattccacgc tgctgccgat ccggtaggcg cctgaaccaa ggacgacaac ggtttctggt
     4441 gtttcctctt tttgtagtcc gacgactgaa gccgtcggtt cacgctgttc ttttcttccg
     4501 acgactaaag ccgtcggttc caaatttaat ccatcctcgg aaccaacggc tttagtcgtt
     4561 ggagtagtaa cattctttgc ggaaaagcca atatcgtgct cgttgccatt gtatgttaaa
     4621 tagagataat tggtccgggc cgggtattct gctgccatgg tgtcaatttg cttcacataa
     4681 ggcaggatgc cttttgcttt gcgataattt cgcactttca gcaggtcatc cgcaatgtga
//
LOCUS       DAOQXS010000203         4727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18390_length_4727_cov_4.055865, whole genome shotgun sequence.
ACCESSION   DAOQXS010000203 DAOQXS010000000
VERSION     DAOQXS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18390_length_4727_cov_4.055865"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKM34_06690"
     CDS             <1..328
                     /locus_tag="PKM34_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ83312.1"
                     /translation="LPLMTAPIPLLSLSPGLPFIITGVILWGIVMGTHETIMKAGIAD
                     ITSIRKRGTGYGIFNMLYGIAIFIGSAAAGFLYDYSINLLITLMIIVEMAALPVFFIL
                     RKHLLN"
     gene            443..1267
                     /locus_tag="PKM34_06695"
     CDS             443..1267
                     /locus_tag="PKM34_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ83313.1"
                     /translation="MDNNQCYINNKFLDYPEAVLHISDLGLQRGYGIFDYFLEMDGRI
                     PFYEDYLDRFYHSAEMMHLQVPLTRELLKEKISFILKSNGFGRSGIKMLLTGGYSDDL
                     YSPVSSNFMIINMPMKHDPCEFSDGVKLLTLEYGRWWPEVKTTFYLPSLVMDKRLKDK
                     GAIEVLYYQNGLVTETTRANFFLVKDGILITAGDGVLKGITRKHVIQVGKEILPVEER
                     PVHLEEIRICDEAFITGTSKHIMPVVEIDEKVIGNGKPGDWTRKISEAFESYYKII"
     gene            complement(1343..2665)
                     /locus_tag="PKM34_06700"
     CDS             complement(1343..2665)
                     /locus_tag="PKM34_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83314.1"
                     /translation="MKKIIFLIALKLSIIEGFSQSCLPDGIDFTNQSQIDNFQADYPG
                     CTEIEGDVEISGSGITNLNGLSVLTQIGGHLYISNNGSLDDLSGLSSLSFIGSSLLIS
                     NNPLLSNLNGINSLGVVEGDVFISNNPVLSDITALGGILPLLVSALRITGNPALSVCA
                     SPFICGFLANPTGKVIIYNNGAGCNNPPEIASQCGITLSCLPFGEYYFFSQDEINAFP
                     GNYPGCIDLKGTFHIEGTDITSLEPLFNIHSLDGTLEIHDNTSLPTLEGLNSLVAVDG
                     AISIVRNDLLTDISSLWQLDTGLISFLVISTNPLLSDCDIQSVCAYLAGPFGYSHNNI
                     TTNNSGCMDKDEVLQECSVNVEEFIFKENTLIITPNPASDKITIRSESPSPQSQISIL
                     NTNGQELFKQTVTGPVTEIDISCLPPGIYFVRLEGEKKLCNGRFVRLD"
     gene            2858..3553
                     /locus_tag="PKM34_06705"
     CDS             2858..3553
                     /locus_tag="PKM34_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNQ83315.1"
                     /translation="MFNLTAICLTLLLFVSNPKEFLDKSVPDTLKEDFTYLVEDYYNN
                     LYHETGLEGKLDYSIFRRALVGISLLDAPRKDILTIIDYTKPSSARRFFVIDLVNGKL
                     LYQTLVAHGKNSGEISCNRFSNREHSLQSSPGFFLTAESYSGRHGYSLRLDGMEPGLN
                     DQARERAIVIHGADYVCQRYVDDFGYIGRSFGCPALPLELNQTIIDLIKGGSCLYVHS
                     SDKNYFDLTTICD"
     gene            complement(3550..>4727)
                     /locus_tag="PKM34_06710"
     CDS             complement(3550..>4727)
                     /locus_tag="PKM34_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNQ83316.1"
                     /translation="WNYGYAPIPDLNALTLKGHITAREIPARLKELRPDMNLYDSLLT
                     ALEKYRRIAAQGGWPAIAGGGKIEPGATDSRIPAIRNRLRLTGELTLRDSISSAKYDE
                     PLEQDIKAFQASHGQFVDGIIGAGTFRELNVPVEKRIESIRINLERVRWVAGNMPRKY
                     ILVNIAAFWLMMVDSNQVVHYTPVVVGKSLSKTPVFRDKMRYIEFNPTWTQPRSIVKN
                     ETIPKLKKDSTYLEKNHMVLLDSKGNLIETSSLDFKNLSPSKFPYIVRQEPGPWNSLG
                     EMKFMFPNEYDIYLHDTPSKSLFSRASRAYSHGCIRVKNPVDLAVKLLETTDHDRKKI
                     MEILKTHETTRVNLPEPLDILLLYWTCGIDRNNRLFFVPDIYERDADVLRELDQVMR"
BASE COUNT         1233 a         1053 c         1161 g         1280 t
ORIGIN      
        1 cctgcccctg atgaccgctc ctattcccct cctttccctg tctccgggcc ttcccttcat
       61 catcaccgga gtcatcctct ggggcattgt catgggaacg catgagacca tcatgaaagc
      121 gggtattgcg gatattacgt cgatccggaa acggggaacg ggttacggga tctttaacat
      181 gttgtacggg attgcgattt tcattggcag tgcggctgcc ggatttttat atgattattc
      241 gatcaattta ttgataacat tgatgataat tgttgagatg gctgcattac cggtattttt
      301 tattttgaga aaacatttgt taaattaata taaccttagt gaaactctgt gtaatctgtg
      361 gtgaatattt cttttgcacc acagattaca cggatttaca cagagtaaac acagagtaag
      421 aacctcaaca cttaaaatcg ctatggacaa taatcaatgc tatatcaaca ataaattcct
      481 cgattacccg gaagccgtgc tccatatttc cgacctgggg ctgcaaaggg ggtatggcat
      541 tttcgattat ttcctggaga tggacggccg gatacccttc tacgaagatt atctcgaccg
      601 attttaccac tctgcagaga tgatgcattt gcaggtgcca ttaaccagag aactattgaa
      661 ggagaaaatc tcctttattc tgaaaagcaa cggtttcggc agatcgggga tcaaaatgct
      721 tctcacggga ggctattcgg atgaccttta ttcgccggtt tcgtccaatt tcatgatcat
      781 caacatgccg atgaagcacg atccgtgtga attcagcgac ggcgtgaagc tgctgacatt
      841 ggagtacgga agatggtggc cggaggtcaa aacgacattt tatcttccat ccctcgttat
      901 ggacaaacgg ttgaaagata agggagccat tgaggtttta tattatcaaa acggtcttgt
      961 tacagaaacg accagggcca atttcttcct ggtaaaagac ggaattttga ttacggcagg
     1021 agatggggtg ttgaaaggaa ttacacgaaa gcatgtcatc caggttggga aagaaatatt
     1081 gccggtagag gagcgaccgg ttcaccttga agaaatccgg atttgtgacg aggctttcat
     1141 tacaggtaca tccaaacata ttatgccggt tgtggaaatc gacgaaaagg taatcgggaa
     1201 tggaaaaccg ggagactgga cgcgcaagat ctcggaggct tttgaaagct actataagat
     1261 aatatgattt attctaacag attcctctcc gctatttgtc tctttcgaac tggctgcttc
     1321 attctgagcg gagcgaagaa tcttaatcca gcctgacaaa tctcccattg cataactttt
     1381 tctctccctc taatcttaca aaataaatcc ccggcggtag acaggaaata tcaatttcag
     1441 taacaggacc agttacagtt tgtttgaaaa gctcttgccc gttggtgttg agaatggaaa
     1501 tttgagattg aggggatggc gattctgacc ggatagtgat cttatccgat gcaggattgg
     1561 gagtgatgat cagtgtattt tccttaaaga taaattcttc cacatttacg ctgcattcct
     1621 gaaggacctc atctttatcc atgcaacccg aattattggt ggtaatattg ttgtgcgaat
     1681 acccgaacgg cccggccagg taagcacaaa ccgactgaat atcgcaatcc gaaagcaatg
     1741 ggttggtgct gatgaccagg aaactgatca aaccggtgtc gagttgccag aggcttgaaa
     1801 tgtcggtcag cagatcattc ctgacgatgc ttatggcgcc atcgactgcc acaagcgaat
     1861 tgagcccttc cagggttggc agtgaagtgt tgtcatggat ttcaagggta ccatcgaggg
     1921 aatggatgtt gaacaatggt tccagactgg ttatgtcagt tccttcgata tgaaatgttc
     1981 ctttcagatc gatgcagccg ggataattac cagggaatgc attgatttcg tcctggctga
     2041 aaaagtagta ttcgccgaaa ggcaggcatg ataatgtaat tccacactgt gaagcgattt
     2101 caggaggatt gttacatcca gcgccattgt tatatatgat gacttttcct gtggggttgg
     2161 ccaggaaacc gcatataaaa ggactggcac aaaccgacag tgccgggttg cctgttatcc
     2221 gtagtgcaga aaccaacaaa ggcaagatac ctcccagtgc tgtaatgtcg ctcagcaccg
     2281 gattattgct gataaacaca tcgccttcaa caacgcccag cgaattaatg ccattgaggt
     2341 ttgacaggag cggattgtta ctgatcaaca gcgagctgcc gataaacgac aaagagctca
     2401 acccgctgag gtcatccagt gagccgttat tgctgatata cagatgacct ccgatctggg
     2461 tcagtacact gagcccgttc aggttggtga tccccgaacc gctgatctcc acatcgcctt
     2521 ctatttcggt acagcccgga tagtctgcct ggaaattgtc gatctgtgac tggttggtaa
     2581 agtcgatgcc atcgggcagg caggactggg aaaagccttc aataatcgaa agctttaacg
     2641 cgataagaaa gatgattttt ttcataaaat ccggataaaa attttttgaa taaaaataat
     2701 gataaaattg ttctgaagat tgttttcacc accgagtaca cagatttgca cagagttgca
     2761 cggagtaaaa tagaatattt gtccgccgga tcaattaata gtaaaatcaa taagaaaaaa
     2821 actatttttg gcttcctgaa aattgattcg gaaatccatg ttcaacctca ctgccatctg
     2881 cctgactttg ttgctgtttg tcagcaatcc gaaggaattc ctggataaat cggtgccgga
     2941 tacgctgaaa gaggatttta cttacctggt ggaggactac tacaataatc tttatcatga
     3001 aacaggcctg gaagggaagc tggattacag tattttccgg agggcattgg ttggtatttc
     3061 attgctcgac gcccctcgca aagatatcct gaccatcatc gattacacca agccgtcgag
     3121 tgcacgccgt ttctttgtta ttgacctggt gaacggcaaa ttgctttacc agacgctggt
     3181 agcacatggt aaaaactccg gtgagataag ctgcaaccgt ttttccaacc gggaacactc
     3241 gttgcagagc agcccgggat tttttcttac agcggagtca tacagcgggc ggcacggtta
     3301 ttcattgcgc cttgacggga tggaaccggg gctgaatgac caggcccgtg agcgggctat
     3361 cgttattcac ggcgccgatt atgtctgcca gcgttatgtg gatgatttcg ggtatatcgg
     3421 ccgcagcttc ggttgccctg cacttccgct cgaactgaac caaaccatta tcgatcttat
     3481 caaaggggga agctgccttt acgtgcacag ctccgacaag aattatttcg atcttacgac
     3541 tatttgcgat taacgcatca cctggtccag ttctcttaaa acatctgcat ccctttcgta
     3601 aatatccggc acaaaaaaca accggttatt cctgtcgata ccgcaggtcc agtatagcaa
     3661 aaggatatcg agcggttcgg gaagattaac gcgggttgtt tcatgggttt taaggatttc
     3721 cataatcttt ttgcggtcat gatcagtagt ttcaagcagc ttcactgcca ggtcgaccgg
     3781 atttttaacc cggatacaac catggctgta ggcgcggctg gcccggctga acaggctttt
     3841 ggaaggggtg tcgtgaagat agatatcgta ttcattgggg aacatgaact tcatttcgcc
     3901 cagcgagttc cagggccccg gttcctgcct gacgatatag ggaaatttag aagggctcag
     3961 gttcttgaag tcaagtgacg acgtttcaat caggttgcct tttgaatcca gcagcaccat
     4021 atgatttttt tcgaggtatg ttgaatcctt cttcagcttg ggaattgtct cattttttac
     4081 aatcgaccgg ggctgggtcc atgtgggatt gaactcgata tagcgcattt tatcgcgaaa
     4141 aacgggtgtt ttgctcaatg atttaccgac cacaaccggc gtatagtgga cgacctggtt
     4201 gctatccacc atcatcagcc agaaagcggc gatattcacc aggatatatt ttcggggcat
     4261 attgccggcg acccagcgga cccgttccag gttaatccgg atcgattcga ttcttttttc
     4321 gaccggcacg ttcaattcgc ggaaggtacc tgcaccgatg atcccgtcga cgaactgccc
     4381 gtggctggcc tggaatgcct tgatatcctg ttcaagaggc tcatcgtatt ttgcagatga
     4441 gatggagtct cgtagagtca gttcaccggt gagacgaagt ctatttctga tagccggaat
     4501 acgggaatca gtggcgccgg gctcaatctt cccacctccg gcgatggccg gccatccccc
     4561 ctgggcagca atccggcggt atttctccag cgccgtcagc agcgaatcgt acaggttcat
     4621 atcggggcgg agttctttca gccttgcagg gatctccctg gcggtgatat gacctttcag
     4681 cgttaaggca ttcaggtcgg ggataggggc gtagccatag ttccatg
//
LOCUS       DAOQXS010000204         4700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18522_length_4700_cov_3.420452, whole genome shotgun sequence.
ACCESSION   DAOQXS010000204 DAOQXS010000000
VERSION     DAOQXS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18522_length_4700_cov_3.420452"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKM34_06715"
     CDS             complement(<1..215)
                     /locus_tag="PKM34_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83317.1"
                     /translation="MKNLLISVILLAMSVTAFSSELFILNTGSVERTKAAFNQNTFVI
                     HFYNDDFIIGSSDDGSPAGSLIIDNNA"
     gene            326..1150
                     /locus_tag="PKM34_06720"
     CDS             326..1150
                     /locus_tag="PKM34_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNQ83318.1"
                     /translation="MGQTEKPWPKWRNDLHRIIFGTDTFEGRLFDIILIFFISASVLV
                     VFLDSIGYIHKQYGRWLYGLEWFFTIVFTIEYIFRILSLRKPWQYVFSFYGLIDFLAV
                     MPTYISLIFTGGQYLIAIRALRLLRIFRVFKLGRFLWASRYLMVAIRNSRHKIVVFLW
                     FVIIIVVVMGSVMYLVEGPENGFTNIPDSIYWAIVTLTTVGYGDISPQTPLGKAIASV
                     IMIIGYGVIAVPTGIFTAELARMHSSSDKTCNHCQNAQNDPDAKYCKKCGQPLQDP"
     gene            1260..3269
                     /locus_tag="PKM34_06725"
     CDS             1260..3269
                     /locus_tag="PKM34_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83319.1"
                     /translation="MKKFYSLIIAISLAFPVFSQTYLSEDFSSGTMPPPGWTIFAFQQ
                     QFVNAPSDSAGGVAPECRIDGFAYNGTLRLMSPAMNMTGVNSVTLIFKHNYDKYASPS
                     PTIGVATKSSGAWNPVWTMVPSSDTGPEEIQVTINNSDLNKPNFQISFYVTGTIQNMN
                     HWYFDNILLMVPLELDGKISGITIPGTITSPQEVGGVFQNIGSTAITDFSVGFEAYNG
                     MIYDTSFTGLNLEMFDAFDFTFDQMWVQPFGTHNINMWINNVNGITDDNPGNDTVTKS
                     ITYIANILPRKVTFEEFTSSTCGPCASFNSGFVPWCESHPDIMLVKYQMNWPGAGDPY
                     YTAEGGTRRNYYGVSYVPDLFYNGARINASVSAVQTSYNQGLALTSYIDIASSFTITG
                     TTINITTNLLPWDNVGNVRVHNIVIEKVTTGNVSSNGETEFHHVMMDMIPDANGASVN
                     LQYATPVKLQYSVNLAGTNVEEYDDLLVAVLVQNQSTKEMLQSEYGEMNTAYSADARL
                     EMINLDGVPLEGFNPDIYEYDVILPEGTLFEPYLEAVLMDDGAMAVVNPAFELPGTAV
                     VDVYAENRFDTKRYLVHYTVFTGVEDKVVPSIQVYPNPVTNVLHVNGMKDAGVKLFNT
                     SGEQVLTLNKFSGSTIDVSELPSGVYIMNVTTADGQTMRRKIVVY"
     gene            complement(3335..4501)
                     /locus_tag="PKM34_06730"
     CDS             complement(3335..4501)
                     /locus_tag="PKM34_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83320.1"
                     /translation="MKTVKITCSILLLLAILNLEKTSGQWAFNGTHIFNTNGGYVGIG
                     TSTPVKLLDVLRNATEPTIRIFNTGGTGGATFQMTDYISGADWKFKATAFGGFKIRDN
                     AHALDVVTIEPNTAANSLYIGAGGNVTIGTDIQTSYVKFQSNQNIIHGIAVSGVSTYT
                     SGSCIGVHGETNTISYAGAGGYFIANNNDGDAPGIYGRADGSSTNTYGVASHAYFGGT
                     GVGAWSYTGDLFRGFDGDFPGGTLRFYVTHAGAVFADGGYNTFKKALLAGGKEEYRSF
                     RMIESTEGWVEDIGSFEMIGGEAVVKIDPVYASSVKLENDYKVFLTPVSEDIVNLVIT
                     KKDPESFRVKGVTNDGRPANCSFDYRIVAKDRVRNSGRMEPVDIPDAINIPRIE"
BASE COUNT         1277 a         1074 c         1042 g         1307 t
ORIGIN      
        1 caggcattat tgtcgataat aaggctaccg gccgggctac cgtcgtcact tgatccgatg
       61 atgaagtcat cattataaaa atgaatgaca aaagtgtttt gattaaatgc agctttagtt
      121 ctctctacag atcctgtatt cagaataaat aattccgaag aaaatgccgt cacactcatg
      181 gccagcagaa taaccgatat taacagattt ttcattttat tcatatttga cgccaaagat
      241 ataaaagtgt tgggtgaaaa attgaatgcg ataaaaagat acaagggttt attcgctatt
      301 tttgataata ctattattga atgcgatggg acagacggaa aagccctggc cgaaatggcg
      361 aaatgacctg caccggatca tattcggcac agatacattt gagggcaggc tattcgatat
      421 catactgata ttctttattt cagcgagcgt cctggtcgtg ttcctggata gtatcggtta
      481 catacacaaa cagtatggaa gatggctata tggccttgaa tggtttttta ccatcgtatt
      541 cacaatagaa tatatattcc gtattctgtc tcttagaaag ccatggcaat atgtttttag
      601 cttctacgga ttgatagatt ttttagctgt aatgccgacc tatatcagcc tgatcttcac
      661 cggcggtcag tatctcattg ccatcagggc actccgtttg ctcaggattt tccgggtttt
      721 taagctgggt cggtttctct gggcatccag gtacctgatg gtagcgatca gaaacagccg
      781 gcacaaaatt gtggtattcc tgtggtttgt catcattatt gtggtggtca tgggttctgt
      841 gatgtacctg gtggaagggc ctgaaaatgg ttttaccaac attcccgaca gcatttattg
      901 ggctatcgta acactaacaa cggtcggtta tggcgatatt tcgccccaga cacccctcgg
      961 taaggctatt gcttcggtca ttatgataat cggatacggt gtgatcgctg ttccgactgg
     1021 tatttttacc gctgaactgg cacggatgca ctcatcatca gacaagacat gcaaccattg
     1081 tcaaaatgcg cagaacgatc ctgatgccaa atattgtaaa aagtgtgggc agcctttaca
     1141 agacccttaa atttaatctt ttcatcctgt tacgtttaaa aaatcaaaat tcaaatcgac
     1201 attatattat atttgcaaca ttaattatca gcaatcatcc tcatcaaaaa aaaaacacta
     1261 tgaaaaagtt ctacagcctg atcattgcta tttcactggc atttccggtt ttcagccaga
     1321 catacctgtc ggaagatttc agcagtggca cgatgccccc tccgggatgg actatttttg
     1381 ctttccagca gcaatttgtc aatgcaccat ccgatagcgc cgggggtgtc gcccctgaat
     1441 gccggattga cggttttgcc tataacggaa cactcagact gatgtcgccg gctatgaata
     1501 tgaccggagt aaattcggtc accctgattt tcaagcataa ttatgataaa tacgcttccc
     1561 catcacccac aattggtgtg gctacgaaat caagcggagc ctggaatccg gtctggacca
     1621 tggtgccctc ttccgataca ggccctgaag agatccaggt gacaatcaat aacagtgacc
     1681 tgaataaacc caattttcag atatccttct atgtcaccgg gactatacaa aacatgaatc
     1741 actggtattt cgacaatatc ctgctgatgg ttccattgga actcgacggc aagatatccg
     1801 gtattaccat accgggtacc atcacctctc cgcaagaagt cggaggtgtt ttccagaata
     1861 tcggaagtac tgcgatcacc gacttcagcg tcggatttga agcctataac gggatgatat
     1921 atgatacttc gtttaccgga ttgaacctgg aaatgtttga tgcgtttgat tttacatttg
     1981 accagatgtg ggttcagcct ttcgggacac acaacatcaa catgtggatc aacaatgtca
     2041 atgggattac cgatgacaat cccggaaacg acacggtgac aaaatccatt acctatattg
     2101 ccaatatttt gccgcgcaaa gttacctttg aagaattcac cagctcgacc tgcggcccgt
     2161 gtgcttcatt caacagcggc tttgtcccct ggtgtgaaag ccatcccgat atcatgctgg
     2221 tcaaatacca gatgaactgg cccggcgccg gcgaccccta ttatacagcg gaaggcggca
     2281 cccgtagaaa ttactacggc gtttcatatg tccccgattt attttataac ggcgcccgta
     2341 tcaatgccag tgtttctgct gtacagacct cttataacca ggggcttgcg ttgacttcct
     2401 atatcgatat cgcatccagt tttaccatta cgggcaccac aatcaacata acaacgaatc
     2461 ttttaccctg ggacaatgtc ggaaatgtca gggtgcataa tattgtgatc gaaaaggtta
     2521 ctactggtaa tgtcagttca aacggcgaga ccgagtttca ccatgtgatg atggacatga
     2581 tccccgatgc caacggagcc agtgtgaacc tgcagtatgc aacaccggtc aaactccagt
     2641 acagtgtaaa ccttgccggc accaatgtgg aagagtatga tgaccttctg gtcgctgttc
     2701 tcgtccagaa tcagtcgacc aaggagatgc tgcagtcgga atacggtgag atgaacactg
     2761 catactcagc cgatgcccgc ctggagatga tcaacctcga cggagttccg ctggaaggat
     2821 tcaatcccga tatttatgaa tacgatgtca ttcttcctga agggacgctc tttgagcctt
     2881 acctcgaagc tgtattaatg gatgacggcg ctatggcagt ggtcaatccg gcttttgaac
     2941 ttccggggac tgcagtggtg gatgtttacg ctgagaatcg tttcgatacc aaaaggtacc
     3001 tggttcatta cacagtattt acgggtgttg aagacaaagt cgttccttcc attcaggttt
     3061 acccgaatcc tgtaacgaat gttctgcatg tcaatggtat gaaagatgcc ggtgtgaaat
     3121 tgttcaatac cagcggcgaa caggttctga cactgaataa attctcaggt agcactattg
     3181 acgtatctga actgccttca ggagtttaca tcatgaatgt aacaacggct gacggacaga
     3241 ccatgagacg gaagatcgtg gtttattaat aagctgataa taaaaaaaaa gagggtgcct
     3301 cgatgagagc accctctttt tttataatat gagattattc aatccgggga atattgattg
     3361 cgtccggaat atcaactggt tccatccggc cggaatttct caccctgtct tttgccacga
     3421 tccggtagtc gaagctgcag ttggcgggcc ttccgtcgtt tgtgacgcct ttaaccctga
     3481 aactttcagg atcttttttt gtgataacca gattcacaat atcttcagat acaggggtga
     3541 ggaaaacttt ataatcattt tccagtttaa ctgaactggc gtaaacggga tcaattttaa
     3601 caacagcctc gccaccaatc atttcaaatg atccgatatc ttcgacccag ccctctgttg
     3661 attcgatcat tctgaaactg cgatattctt ctttacctcc agctaataag gcctttttaa
     3721 aggtgttata gccaccatcg gcaaaaacag cccctgcatg ggtcacatag aaccgcaagg
     3781 tgcctccggg aaaatcgccg tcgaaacctc tgaaaagatc tccggtgtag ctccatgctc
     3841 cgactcctgt gcccccaaaa tacgcatgac tagccacacc gtatgtattt gtactgctgc
     3901 catctgccct gccatatatt ccgggtgcat ctccgtcatt attatttgct atgaagtagc
     3961 ccccggcacc ggcataagag attgtattgg tttcgccgtg cacacctatg cacgagccgg
     4021 aagtatatgt agacacacca gaaactgcga tcccatggat tatattttga ttggactgaa
     4081 atttcacata ggaagtctgt atatctgtgc ctatagtaac attgcctccg gcaccaatgt
     4141 aaagtgaatt cgccgctgtg ttgggttcga tggtcacaac atccagggcg tgtgcgttat
     4201 ctctaatctt aaaaccgcca aaggccgtcg ctttgaattt ccagtcggcg cccgaaatgt
     4261 aatcggtcat ttgaaaggtg gcgcctcctg ttccgcccgt attaaatatc cggattgtcg
     4321 gttcagttgc atttcggagc acgtccagca attttacggg cgtgctggtt ccgataccaa
     4381 cataacctcc gtttgtattg aagatgtggg tgccattaaa ggcccactgc cccgaggttt
     4441 tttcaaggtt gagaatggct agcaataata aaatgctaca ggtgattttt acggttttca
     4501 taagaagatg aattagcaat gccttaaaga taaggaatat tgtggcataa tgtatatgaa
     4561 gtaatgtaaa aataaacttt cagagaaatg aatctccaaa ggttgagcta tgcggtctgg
     4621 acagcttgtc ccgagtgctc gggatgactc gaacccgcga ccttcccgag gactcgggac
     4681 aggcattcta accaagtgaa
//
LOCUS       DAOQXS010000205         4699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18527_length_4699_cov_3.661068, whole genome shotgun sequence.
ACCESSION   DAOQXS010000205 DAOQXS010000000
VERSION     DAOQXS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18527_length_4699_cov_3.661068"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKM34_06735"
     CDS             complement(<1..313)
                     /locus_tag="PKM34_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83321.1"
                     /translation="MAENFLRILVCPLDWGLGHATRCVPVITKMVEEGHQVTLAADGQ
                     ALGFLRSQFPDLPWKRFPGVTIRYPSGKSMVLKMLLQLPGIFLNIILENQRIKKIAGE
                     CR"
     gene            364..1470
                     /gene="dprA"
                     /locus_tag="PKM34_06740"
     CDS             364..1470
                     /gene="dprA"
                     /locus_tag="PKM34_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ83322.1"
                     /translation="MQNKDSLIYAIALTLIPGIGDVQGKKLVAYCGGVEAVFREKKKD
                     LMRIPGIGNATADAIHRKGIFARAEEEIGFIRQYGINALFYLDPDYPERLKHCEDGPI
                     MVYFKGNAGLSRKRVLSIVGTRNPTDYGKSMCEQIIRDLSDLGVMTVSGLAYGVDTLA
                     HRASLAAGIPTVGVLAHGLDLIYPFVNRPLAGKMIENGGLMTEFMSHTKLNRDYFPRR
                     NRIIAGMADATLVIESAAKGGALITAEIAGSYNRDVFALPGRATDLKSSGCNNLIRNN
                     KAALVQSAADICYLMGWDQDNHRAHSQPDLFTVMNEDEKKIYDLLMRLKEAGIDEIYL
                     QSGMTQSKVSSILLKLEFDGLVKAFPGKRFRLNN"
     gene            complement(1473..2156)
                     /locus_tag="PKM34_06745"
     CDS             complement(1473..2156)
                     /locus_tag="PKM34_06745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNQ83323.1"
                     /translation="MEFLNYIYFVIPPAIAVLVFFYAKALQNEWPAGLLIRAFVWGMF
                     SIVLVLTVQVIAAVFDLDNLTNLRRILFYSLVIMAFFSELGKFTILKVFFYPKKEFRT
                     PVDGILVSVMISLGFATMNNILSFVNIPHLSVSSVNALSAGPANVIFGLMMGFFIGLG
                     KLRKMRLVDSMTGLAAAIFFHALYDFCLLTRDYKLLWAFFIGSAIIAVSLWIAALRIH
                     RDALEEGNF"
     gene            2223..3143
                     /gene="rsgA"
                     /locus_tag="PKM34_06750"
     CDS             2223..3143
                     /gene="rsgA"
                     /locus_tag="PKM34_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNQ83324.1"
                     /translation="MEGIVTRSTGSWYMVTSDEGMVTPCKLKGNFRLKGIRTTSPLAV
                     GDRVEYEMTPGNDAGVIYRIFDRHNYIIRKATKLSRESHIIAANIDQLLLVATLAFPR
                     TSTGFIDRVLVTSEAYHIPAILVFNKIDMIVEELKEGLREMIEIYEHAGYRCLQVSAR
                     SGAGLDELMDAMKDRTSLLSGHSGVGKTSLINALQPGINLKIKEISSYHLKGQHATTF
                     PEMHRLSFGGYIIDTPGIKEFGLTDFGQFEVAERFPEMRALMHLCRYGNCTHSHEPGC
                     EVIRAVNDGRIHPSRYSNYLGILNGRDWTE"
     gene            3164..3826
                     /locus_tag="PKM34_06755"
     CDS             3164..3826
                     /locus_tag="PKM34_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ83325.1"
                     /translation="MIAKEIITFDILPLNVNDSCSFALNAMDENRVNHLPLVRERELI
                     GLVSEFDLVNHPDQSEPLGNVRLSMPNAFISSYQHVFDVMKMMTELKLSLLPVVDQRN
                     LYQGAITLSALVRYLTLNMSVLNPGGMIILEVAESNYSMAEISQIVESNDVRIIGAFF
                     STKADSKLIDLTLKINATDLNPVIQTFERYHYTIKATFAEKDDLDELKERYDSLMNYL
                     NV"
     gene            3838..>4699
                     /locus_tag="PKM34_06760"
     CDS             3838..>4699
                     /locus_tag="PKM34_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83326.1"
                     /translation="MSIDPAKYAIGPVWLFLALFLALAGIGHAQETDGSQDKGDRFIS
                     IDKIVLEGNRITRDPIILREIVFKEGDFIRSAALDSLIGCSRNNLLNTLLFNFVEINA
                     NHTDSSGSSVVVGVKVVERWYIWPTPIFKLSDRNFNVWWETRDPERLSYGFYIDWKNF
                     RGRKEELILRFQWGYDRLIQLQYQVPYLNRSKTMGLGVGFGFARQKESAFRTLNNKQE
                     FIRLDSGFARKDLFAYSQLTVRNNIHITHTIQAGFDQHHFSDSLIRSNPDFTNDTSTV
                     IRYFSISYQYK"
BASE COUNT         1299 a         1119 c         1125 g         1156 t
ORIGIN      
        1 cccggcattc tccggcaatc tttttaatgc gttgattttc caggatgata ttcagaaaga
       61 taccgggaag ctgaagcaac attttcaaaa ccatgctctt ccccgaagga taacggattg
      121 taactcctgg aaaccgtttc catggcagat cgggaaattg actcctaaga aatccgaggg
      181 cctggccatc cgcagcgagc gttacctggt gaccttcttc caccattttt gtgatcacag
      241 gcacacaacg cgtcgcatga cccagtcccc aatcgagcgg acagacaagg attcggagaa
      301 aattttcagc caaccgggta ttttttgccc gacaaaggta ttaataagaa aaacctgttg
      361 attatgcaga acaaagattc attgatttat gccatagccc tgacgctgat accgggtatc
      421 ggggatgtcc agggtaagaa actggttgcc tattgtggtg gggtggaagc ggttttccgg
      481 gagaagaaga aagatctgat gcgaattccg gggatcggca acgctaccgc cgatgcgatc
      541 catcgcaagg ggatctttgc cagggcggaa gaggagattg gcttcatccg tcaatacggt
      601 attaatgcat tattttacct tgatcccgat tatcccgaaa ggctgaaaca ttgcgaagat
      661 ggcccgataa tggtctattt taaaggaaat gccggactct cccggaaacg cgtacttagt
      721 atcgtcggta cacgtaaccc gaccgattac ggcaaatcca tgtgcgaaca gatcatccgc
      781 gatttgtcgg acctgggcgt tatgacggtc agcgggctgg catatggtgt cgatactttg
      841 gcccaccgcg cctctctggc cgccggtatt cctacggtag gcgtgctcgc tcatggcctc
      901 gacctgatct atccctttgt caatcgtccc ctggccggga aaatgatcga aaacggtgga
      961 ttgatgaccg aatttatgag ccataccaaa ctgaaccgtg attactttcc gcggcgcaac
     1021 cgcattattg cgggtatggc cgatgcgacc ctggttatag aatccgctgc caaaggagga
     1081 gcactgatca cggcggaaat tgccggctct tacaacaggg atgtttttgc ccttccgggc
     1141 cgggcaaccg atttgaaatc aagcggatgc aataacctga tcagaaacaa caaagcggca
     1201 ctcgtgcaat ccgctgccga tatctgttat ctcatggggt gggaccagga taaccatcgg
     1261 gcgcacagtc agcccgatct ttttaccgta atgaacgagg atgaaaagaa gatctatgat
     1321 cttctcatgc ggctgaaaga agccggaatc gatgaaatat acctgcaatc gggcatgacg
     1381 cagagcaaag tctcgtcaat attgctgaaa ctggagtttg acggattggt gaaagctttt
     1441 cccggtaaga gattcagact gaataactaa aatcagaaat tcccttcctc aagggcatct
     1501 ctgtgaatcc gtaatgccgc aatccataat gagacggcta tgatggccga ccctataaaa
     1561 aaagcccaaa gcagtttata atccctggtc aggaggcaaa aatcgtacaa agcatgaaaa
     1621 aagatcgctg ctgcaaggcc tgtcatggag tcgaccagcc gcatcttcct caattttccc
     1681 aaaccgatga agaatcccat catcaaaccg aaaataacat tggccgggcc ggcgctgagt
     1741 gcgttgacgc tgctgacaga cagatggggt atgttgacga aggagagtat gttgttcatc
     1801 gtggcgaaac ccagcgatat catcactgaa acgaggatac catcgacggg agtcctgaat
     1861 tcttttttcg gatagaaaaa aaccttgaga atggtaaact ttcccaattc agagaagaaa
     1921 gccatgatca cgagtgaata aaacaagatt cgcctgaggt tggtcagatt atcaaggtca
     1981 aaaaccgcag ctataacctg gacagtcagc acaagcacaa tactgaacat tccccagaca
     2041 aaggcacgga tcagcaatcc ggccggccat tcattctgta gggctttagc ataaaaaaat
     2101 accagaacag caattgccgg tggaatgaca aaataaatgt aattcagaaa ctccataaga
     2161 ttgaaggtta tcggggattc aaaattagca attgtttgta attttacaaa ttaaaatatt
     2221 tcatggaagg gatcgtcaca cgttcaacgg gcagttggta tatggtaact tctgacgaag
     2281 ggatggtcac cccctgcaag ttgaaaggca atttccggct gaaaggcatt cgaacaacca
     2341 gcccgctggc cgttggcgac cgtgttgaat acgaaatgac tccgggaaac gacgcaggtg
     2401 tgatttaccg tatttttgac cgtcataatt atataatccg caaagcaacc aaactttccc
     2461 gggaatcaca tatcatcgca gccaacatcg accagttgct cctggtggcc actcttgcat
     2521 ttcccaggac atccaccggc tttatcgacc gtgtactggt cacttcagag gcttatcata
     2581 ttccggccat cctcgtcttc aataaaatag atatgatcgt tgaggaactg aaagaagggc
     2641 tccgggagat gatagaaata tatgagcacg ccggttacag gtgcctgcag gtatcggcca
     2701 ggagcggggc agggctggat gaactgatgg atgccatgaa agaccggacc agcctgcttt
     2761 caggtcattc cggtgtgggt aaaacgagcc ttatcaatgc cctgcaaccc ggcattaacc
     2821 tgaaaataaa ggagatatca tcctatcacc tcaaaggcca gcatgccact acatttcctg
     2881 agatgcaccg cctctctttc ggcgggtata taatcgatac tcccggaatc aaggaattcg
     2941 gcctgaccga tttcgggcag ttcgaagtcg ccgaacgatt tcccgaaatg agagccctca
     3001 tgcatctttg ccgttatggc aattgcactc actcacacga accgggttgt gaagtgatcc
     3061 gtgcagtcaa cgacggaagg atccatcctt cgaggtattc caactacctt gggattttga
     3121 atggccgtga ctggaccgaa taatcatttt aaaacaaatt gttatgatcg ccaaagaaat
     3181 aatcaccttc gatattctgc ccctgaatgt gaatgattct tgctctttcg cccttaatgc
     3241 catggatgag aaccgggtca accatctgcc gctggttcgt gaaagggaat tgatcggact
     3301 tgtgtcggaa ttcgacctgg ttaaccaccc cgatcaatct gagccgctcg gaaacgtcag
     3361 gctctccatg ccgaacgcgt tcatctcttc gtaccagcat gtgttcgatg tgatgaaaat
     3421 gatgaccgaa ctgaagcttt cgctgctccc ggtcgttgac caacggaatc tttaccaggg
     3481 agccatcacc ctttccgctt tggtcagata tctcacattg aatatgtcgg tgcttaaccc
     3541 gggcgggatg attatactgg aggtggctga aagcaattat tcaatggccg agatatccca
     3601 gatcgtagaa tcgaacgatg tgcggatcat cggtgctttt ttcagcacca aagcagattc
     3661 taagcttatc gaccttaccc tcaagatcaa tgcaacagac cttaatcccg tcatccagac
     3721 attcgaaaga tatcactata caattaaagc cacttttgcc gaaaaagacg acctggatga
     3781 actgaaagag cgttatgatt cgttgatgaa ttacctcaac gtatgaaatg atttcatttg
     3841 agcattgatc cggcaaaata tgctatcggg cctgtctggc ttttcctggc gctgttcctg
     3901 gcactcgcag gcattggcca cgcccaggaa acagacggga gccaggataa aggcgaccgc
     3961 tttatttcca tcgacaaaat tgttctcgaa ggtaaccgga tcacaaggga tcccattatc
     4021 ctgagggaaa ttgtattcaa ggaaggtgat tttatccgtt ctgccgccct cgactcattg
     4081 atcgggtgtt caaggaataa cctgctgaat acgcttcttt tcaattttgt cgaaataaac
     4141 gccaatcaca ctgattcgtc agggagtagt gttgttgtcg gggttaaagt ggttgagaga
     4201 tggtacatct ggcctacacc catattcaaa ttatcggaca gaaattttaa cgtatggtgg
     4261 gaaacccgtg atcctgaaag gcttagttat ggattttata tcgactggaa aaatttcagg
     4321 ggaagaaaag aggaattgat cttaaggttc cagtggggat atgaccggtt gatacagctc
     4381 cagtaccagg tgccttatct taaccggtcg aaaacgatgg ggttgggcgt cggttttggt
     4441 tttgcccgcc agaaggaatc ggcattccgg acgctgaaca ataaacagga gtttatccgc
     4501 cttgactccg ggtttgcgcg aaaggatctt tttgcctaca gccagttgac ggtccgcaac
     4561 aatattcata ttacccacac tatccaggcc ggattcgacc agcatcattt ttccgacagc
     4621 ctgatccgct ccaatcccga ttttaccaac gacacttcta ccgttatacg ctatttttca
     4681 atttcttacc agtataaaa
//
LOCUS       DAOQXS010000206         4655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18785_length_4655_cov_3.218043, whole genome shotgun sequence.
ACCESSION   DAOQXS010000206 DAOQXS010000000
VERSION     DAOQXS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18785_length_4655_cov_3.218043"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKM34_06765"
     CDS             <1..646
                     /locus_tag="PKM34_06765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNQ83327.1"
                     /translation="LRNFAFPYFARDIAEFWRRWHISLTTWFRDYLYIPLGGSRGGKW
                     MSVRNTFIVFLVSGFWHGANWTFIVWGFLNALYFMPLLLLNKNRQNLEIAAKGKWLPS
                     VRDLAGMVVTFSLTLIAWVFFRADSVSGALIFIQDIFSASLFTAPKIRTHYIWVFILI
                     LLVFEWIFREKQYGLQFNEVRLHRSLKWSFMLALALVIFIFGNFNKTEFIYFAF"
     gene            650..1561
                     /locus_tag="PKM34_06770"
     CDS             650..1561
                     /locus_tag="PKM34_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83328.1"
                     /translation="MKKYLINTAIFILAFLILVIALALASSRLVYKRAFRNWEVEGNL
                     LVMKSNHDYNLLMMGISHARNFSRHKNHLRMEKILHQEIINIGIGEGKCGAIDQHFFL
                     EYFYYKRNSADRIFYVMSPTLLNKGFINRASNTFEFEPFRLDFFIKYLGYKSDNKYER
                     LFYYVKSKFARKWYRLQPRSLDEKKSALKEIDSTKIKEGLKLLLVDGERMDEFQRNCL
                     VVEETIKLAKEHNTEVIFFIPPALFGKWEGHYETMDFLEEMYKKYGTRYYDFSESVLI
                     PEYYYDHHHLNSKGIEYFAENYLKGIL"
     gene            1585..3033
                     /locus_tag="PKM34_06775"
     CDS             1585..3033
                     /locus_tag="PKM34_06775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNQ83329.1"
                     /translation="MLFNSISFAVFLPVVFLLYWFVARKSLRGQNVLLLIASYFFYGS
                     WDYRFLFLLIFSTFLDYFTGIKIHEAETRKRKKIWLWLSITINLGFLGVFKYYNFFAA
                     SFAGALSLVGFHADYFTLQVILPVGISFYTFHGLSYVLDLYNDRIKPERDFINYAVFV
                     SFFPLLVAGPIERATHLLPQIIRKRDFDYHQAMDGLRQMLWGFFKKMVIADNCAIYVN
                     DIFSHSSGYSGSTLVLGTMFFAFQVYGDFSGYSDIALGTARLFGINLLRNFAFPFFAR
                     DIAEFWRRWHISLTTWFRDYLYIPLGGNRGGKWMSIRNMFIVFLVSGFWHGANWTYIV
                     WGLLNAIYFLPLLLLHKTKQNVEIVAKGRLFPSLKELTGMIVTFALILFSFIFFRARD
                     LHHAFAYTAKIFSPSLFSLPHFKGDEKIAPLAILILVFFLIEWIGREEQYAIEKIGLK
                     WHKAVRWALYYTLVMAIFLFAGKEQAFIYFQF"
     gene            3036..3947
                     /locus_tag="PKM34_06780"
     CDS             3036..3947
                     /locus_tag="PKM34_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83330.1"
                     /translation="MKKFLAEVLVFLIILLISFYLTFMRANGKTDPFYLRFTSPKQHS
                     LILGTSRAAQGIQPSVLDSILPGSGFYNYSFTIGHSPYGPAYLNSIKKKLDHQTKNGI
                     FILSVDPWSISSNANDPNNPDRFRERKLAIGKTPVVNIRPNFFYLINAYDKPFYHLLP
                     RQKSSIILRDDGWLDVKFKIDSLKMEQLIEKKVDSYRKNNLKKYKFSDVRLEYLTKTI
                     TYLKNFGRVYLVRLPVHPRMMEIDEEFIPDFDQKIRQISEQTGTPYFDMSFMNDSILY
                     TDGNHIYRESGKVVSARIGEWIAGQEQ"
     gene            complement(3960..>4655)
                     /locus_tag="PKM34_06785"
     CDS             complement(3960..>4655)
                     /locus_tag="PKM34_06785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83331.1"
                     /translation="CKENLVKYFGKEGIWCPIGPLVRDAVMQEDNILNIINLSDHNWH
                     NIINDEEWFTGVPRCAGKVPVIGRHTRDQYVKWPERIEDLLRVYPDDDKFAVKVLGGA
                     KTVEERLGRIPDNWEVYPFNSKNTREFLSEIDFCVYFHHKDLVEAFGRTPLEAMAAGV
                     PVILPEHFRVLFREAAIHARPEEVKDIVYKYFLDKSLYQDQVEIARKFVKQNFSYSQH
                     INRIRPFVKKLQG"
BASE COUNT         1215 a         1073 c          994 g         1373 t
ORIGIN      
        1 gctccgcaat tttgccttcc cgtactttgc ccgtgatatt gccgaattct ggcgcagatg
       61 gcatatctcg ctgaccacat ggttccgcga ttacctctat atcccgctgg gaggaagccg
      121 aggcggcaaa tggatgagcg tcagaaatac gtttatcgtc ttcctggtca gcggattctg
      181 gcatggcgcc aactggacct tcatcgtctg gggtttcctg aatgcactct attttatgcc
      241 gctgctttta ctgaataaaa accggcagaa cctggaaatc gcggctaaag ggaaatggtt
      301 gccctcagtg agggatcttg ccgggatggt cgtcactttt tcgcttactt tgatcgcctg
      361 ggtgtttttc cgggccgaca gtgtcagtgg ggcattaatc ttcatccagg atatcttcag
      421 cgcttccctg ttcaccgccc cgaaaatcag aactcattat atctgggtgt ttatcctgat
      481 tttgcttgta tttgagtgga tattccgtga aaaacagtat ggattgcagt tcaatgaggt
      541 acggctccac agatcgctga aatggagctt catgctggcg ctggcgctgg tcatttttat
      601 cttcggtaat tttaataaaa ccgaatttat ttatttcgct ttttaaaaga tgaagaaata
      661 cctgatcaat acggcaatct ttatcctggc cttcctcatc ctggtcatcg cgctggcact
      721 ggccagttcc cgtcttgttt acaaaagagc attccggaac tgggaagtgg aaggaaacct
      781 tctggtcatg aaatcgaatc atgattataa tcttttgatg atggggattt cgcacgcccg
      841 gaacttctcc cggcataaaa accacctgcg aatggaaaag atattgcacc aggagataat
      901 aaatatcggt atcggcgaag gaaaatgcgg tgctatagac cagcattttt tcctggaata
      961 tttttattac aagagaaact ctgcagacag gatcttctat gtcatgtcac ctacactgct
     1021 gaataaggga tttatcaaca gggcttcaaa tacctttgaa tttgaacctt tccgtctcga
     1081 tttcttcatt aaatacctcg gctataaatc agacaataaa tatgaacggc tgttttatta
     1141 tgtaaaaagc aagtttgcgc gcaaatggta ccgtttgcaa ccccgttcac ttgacgaaaa
     1201 aaaatccgcg ctgaaagaga tcgattcaac gaaaatcaag gagggattaa aactactgct
     1261 cgttgatggg gaaaggatgg atgaattcca gcgaaactgc ttggtagtag aagaaacgat
     1321 aaaactggca aaagaacaca acaccgaggt catctttttt attccgccgg cgctctttgg
     1381 gaaatgggag gggcattatg aaacgatgga ctttttagaa gagatgtaca aaaaatacgg
     1441 cacccgctat tacgattttt ccgaatcggt tcttatccct gaatattatt acgaccatca
     1501 tcatttaaat tcaaaaggaa ttgagtattt tgcggaaaat tacctgaaag ggatattata
     1561 aaaggttagc ttaactgcag cgctatgctg ttcaattcaa tatcatttgc tgtctttctg
     1621 ccggtcgttt tcctgttgta ctggtttgtc gcgcgaaaaa gccttcgggg ccagaatgtc
     1681 ctgttgctca tcgccagcta tttcttttat gggagctggg attatcgctt ccttttcctg
     1741 ctcatctttt ccactttcct cgattatttt accggtatca agattcacga agcagagacc
     1801 aggaagcgga aaaaaatctg gctgtggctc tccatcacga tcaaccttgg atttctcggc
     1861 gtattcaagt actacaactt ttttgctgct tcatttgccg gtgcgctatc actggttgga
     1921 ttccatgccg actatttcac ccttcaggtt atcctgccgg taggcatctc gttctacaca
     1981 tttcatggcc tgtcgtatgt cctggatctt tacaacgacc ggatcaaacc ggaaagggac
     2041 ttcatcaact atgccgtttt tgtcagtttt ttccccctgt tagtggccgg ccccatcgag
     2101 cgggctactc accttctccc ccagatcatc aggaaaagag atttcgatta ccaccaggcg
     2161 atggatggcc tcaggcaaat gctctgggga ttctttaaaa agatggtcat cgccgacaat
     2221 tgcgccattt atgttaatga tattttcagt cattcatctg gttattccgg cagcaccctg
     2281 gtgcttggta cgatgttctt tgcattccag gtttatggtg acttttcggg ttattccgac
     2341 attgctctcg gaacggccag attgtttggt atcaacttac taagaaactt tgcatttccc
     2401 ttctttgccc gcgacatcgc cgaattctgg cgacggtggc atatatcgct aacgacctgg
     2461 ttcagagatt atctctatat tcccctcggt ggcaacaggg gcggtaaatg gatgagcatc
     2521 aggaatatgt ttatcgtctt cctggtcagc ggattctggc atggagcaaa ctggacctac
     2581 attgtgtggg gactcctgaa tgccatttat tttcttccat tgctcctgct tcataaaacc
     2641 aagcaaaatg tcgaaatcgt tgccaaagga cggctctttc cttccctgaa agaactgacg
     2701 gggatgatcg tcacttttgc cttaatattg ttttccttta tatttttccg ggcccgtgat
     2761 ctgcatcatg cattcgctta taccgctaaa atattttcgc cttcactttt cagcctgcct
     2821 cattttaaag gcgacgaaaa aatcgcacct ctggctattt taatacttgt cttttttttg
     2881 atcgaatgga ttggaagaga agaacaatat gctattgaaa aaatagggct gaaatggcat
     2941 aaagctgtaa gatgggccct ctattacacc cttgttatgg caatcttttt atttgcaggt
     3001 aaagaacagg cttttatcta ttttcagttt tagttatgaa aaaattcctg gcagaagtcc
     3061 tcgtttttct gatcatttta ctgatcagtt tttatctgac tttcatgagg gccaacggga
     3121 agaccgatcc tttctattta cggtttacct caccaaaaca gcactcgctc atcctgggca
     3181 cctcgcgtgc cgcccagggg atacaaccat cggtactcga ttcgattttg cccggaagcg
     3241 gattttataa ttactctttt acaatcgggc acagccctta tgggcctgca tacctgaata
     3301 gcatcaaaaa gaaactggat catcaaacta aaaacggaat ttttatcctc tccgttgatc
     3361 cctggagtat ttcatcaaat gcaaatgatc caaacaatcc ggatcgattt agagaaagaa
     3421 aattagccat cggaaaaacg cctgtcgtga atatccgccc aaacttcttt tacctgatca
     3481 atgcttacga taagcctttt tatcatttgc ttcccaggca aaagtcatcc attatcttac
     3541 gcgacgatgg ctggctcgac gtaaaattta agattgactc cttaaagatg gaacagttga
     3601 ttgaaaagaa ggtcgactct tacaggaaaa acaacctgaa aaaatataaa ttctctgatg
     3661 tgaggcttga atatcttacg aaaaccatta cctatcttaa aaatttcggc cgggtttacc
     3721 tggtgcgatt gccggtccat ccccggatga tggaaatcga tgaagaattt atccctgatt
     3781 tcgatcagaa aatacggcaa atatcggaac aaaccggtac tccttatttt gacatgtcct
     3841 ttatgaatga ttccatcctg tatactgacg gaaatcatat ttacagggaa tccgggaaag
     3901 tggtctcagc ccggatcggc gaatggatag caggtcagga gcagtaggaa ccgacggctt
     3961 tagccctgca gtttcttcac aaacggcctg attcggttga tatgctgcga atagctgaaa
     4021 ttctgtttca caaatttacg ggcaatctcc acctggtcct ggtataaaga tttatccaga
     4081 aaatatttgt acacgatatc tttaacctcc tccggtctgg catggatcgc ggcctcacgg
     4141 aacaataccc ggaagtgctc cggcaaaata accggcacac cggcagccat ggcctccagt
     4201 ggcgtccgtc caaatgcttc taccaggtct ttgtgatgaa aataaacaca gaagtcgatt
     4261 tcggagagaa actccctggt gttctttgaa ttgaaaggat atacttccca gttatcagga
     4321 atccggccca gtcgctcttc caccgtttta gcgcctccca gcactttcac tgcaaatttg
     4381 tcatcgtcgg ggtaaacccg gagaagatct tcaatgcgtt caggccattt tacgtactgg
     4441 tcgcgcgtat gccttccgat aacaggtacc ttacctgcac accggggtac acctgtgaac
     4501 cactcctcat cattaataat attatgccag ttatgatccg acaggttaat aatattcagg
     4561 atgttatcct cctgcatgac agcatctctg accagtgggc caatcgggca ccagatgcct
     4621 tccttgccaa agtatttaac gaggttttct ttaca
//
LOCUS       DAOQXS010000207         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18793_length_4654_cov_3.262231, whole genome shotgun sequence.
ACCESSION   DAOQXS010000207 DAOQXS010000000
VERSION     DAOQXS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18793_length_4654_cov_3.262231"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1158
                     /locus_tag="PKM34_06790"
     CDS             214..1158
                     /locus_tag="PKM34_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HNQ83332.1"
                     /translation="MKSITAIFRLLTIALPLAVVGQQLENPGFEEWETAAPSIIEPTE
                     WNSIKTADDPGIAAMAPITIDRSEDAHTGNYSLKLYNVTPFPNLVATGAITNGRFHAE
                     FDLTKSYSYTQRSDPKYHTSFTWRPDSLTGWFKYFPNLNDAAQFKVILHVDSCKLPTD
                     SLSQLNIVGMGVFVTEHGVTYDSWTRFSVPFQYFNDSLPEYALTTMNSGDSITAIDGS
                     YLLIDDLELKYAPAGINEPKKPDSFLTYGKGILNLTLAPETYMNQWLYIIDASGQTVY
                     STRLEKGTINLPESIPGAVYIALLITSNRQYAQKILIP"
     gene            1162..3555
                     /locus_tag="PKM34_06795"
     CDS             1162..3555
                     /locus_tag="PKM34_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83333.1"
                     /translation="MKKIVLLLAVLLTAVYAFSQDKGTFRGIVRDKTNNEPLIGANII
                     LKLDRSFGSASDIDGRFSLRLDPGSYIFEISYTGMKSDSVSVRINPGETVEREILLEP
                     FVSQLQGVEVKAGKFDRPIEEMTVSMEIIKAGFIEAKNTQNVQTILDYTPGLNILDNE
                     PQIRGGSGFTFGVGSKVALLVDDMPMLSGDAGRPYWDFVPVENIEQIEVIKGASSVLS
                     GTSALSGAIHIRTALPNDKPLTKLTVFSGLYTTPEDKEQKWWDDVPYLTGANFLHTRK
                     VDNLDVVLGGNAFFDHSYIGAPRTVPPVYDTTENFADNKMATQKVRFNFNLRKRSESY
                     QGLNYGINGNFMYQHAPMVLAWLDDTAGFFRGYPGATILQDQFIGNLDPFINFYSKVG
                     FRHSFKARALYTNNHQSNGQHLESTFIFADYNFRREYDFLQGLEFIGGLSGQYTLSNS
                     DIYKGSGSEINRFLNFSGYTQFENNLFKTLTLSVGLRLEYYKMNEAASSFEPIFRAGF
                     NLKVMKETYVRISYGQGYRYPTIAEKFIRTDVGTFSVFENDTLQPERSWNAEVGVKQG
                     FKFFNYFGYFDFAVFQQEYWNTIEYLFGRWVAPTADISGYGFRFLNTGHSRVTGVDIS
                     VTGAVNLFKKVSMKTQMGYNYILPVSLQPDFVYASDIYTTYSYNTTSLDSTQGILKYR
                     FLHTFKGDIAFDIYDFTLGFSAKYFSRIENLDKSIADFEKYTASTGGTLQQIRYMDYF
                     NQYNNGQWILDIRAGYALSDHNEFSLLVNNMLNNIYSLRPLKAEPMRSVQLQYIHKF"
     gene            3572..>4654
                     /locus_tag="PKM34_06800"
     CDS             3572..>4654
                     /locus_tag="PKM34_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNQ83334.1"
                     /translation="MDNAILLTKIIATLGPSSSSVELVGQLIREGVRVFRVNFSHGSI
                     DSFSRIIDNVRQAAALSGVPAAILGDLSGPKIRIGQVPDPGIELIKGEKVEFTAQDTP
                     AFRKEDGTVVFSTTSPEIVCEVRTGQRIMIDDGNVVLICTGKHEDRLVCEVMQAGRVS
                     SKKGINLPETDLTVAAMTEWDDRCVEFAVAKKVDYLALSFVRHAKDIIHLKEILAALG
                     ARPFSPFEYHADDMSGVPDDVQFIPIISKIEKPQAIDDLDGIVAESDAIMIARGDLGV
                     EMELAEVAVLQKKIISACHDQGVPVIVATQMLQSMIESPVPTRAEVSDVANAIYDGVD
                     AVMLSGETAVGRYPVETVRMMARIARL"
BASE COUNT         1258 a         1098 c         1145 g         1153 t
ORIGIN      
        1 tcgacgaatg cacgatcaat tacgataccg gaatgttccg gttcaacctg gtcaggagga
       61 ttcccaccaa aaaaccgata aggctgctca actcatgaaa aatccgacgg ttcataaata
      121 ctcatactaa atatccataa taatttggat ttttataatt ttaccctccc gaaagtcaat
      181 tttttaatgt ttaaccataa actaagccca gatatgaaaa gcattacagc catattccgg
      241 cttttaacca tcgccctgcc gctggcagtt gtgggacagc aactggaaaa tcccggattt
      301 gaggagtggg aaacagcagc gcccagtatt attgagccta cagagtggaa cagcatcaag
      361 acggccgatg acccgggaat tgcagccatg gcgccgatca ccatcgaccg gagtgaagat
      421 gcccacaccg gtaactattc cctgaaactt tacaatgtca ctcctttccc aaacctggtt
      481 gccacgggag ctattaccaa cggaaggttt cacgcggaat tcgatctcac caagagctat
      541 tcatatacgc aaaggagcga tccgaaatat cacaccagct tcacctggcg tccggacagt
      601 ctgacgggat ggttcaaata tttccccaac ctgaacgatg cggcccagtt caaagtgatc
      661 ctgcacgttg actcatgtaa attaccaacc gactcattat cacaactcaa catcgtaggc
      721 atgggcgttt ttgtcactga acacggtgtg acttatgatt cttggacccg cttttcggta
      781 cctttccaat atttcaatga cagtttacct gaatatgcgc tgacgactat gaattcaggc
      841 gacagcatta cagccatcga cgggagttat cttttgatcg acgatctgga actcaaatat
      901 gctccggcag gtatcaatga acccaaaaaa ccggactcat ttctcaccta tggcaaaggt
      961 attttaaatc tgacccttgc cccggaaact tatatgaacc aatggcttta tattattgat
     1021 gcatcgggac agaccgttta ttcgacacgg ttggaaaagg gtacgataaa ccttcctgaa
     1081 agcatacccg gtgccgttta tattgcatta ctgataacca gtaacaggca atatgcccaa
     1141 aaaatattga tcccataaag aatgaagaaa attgtcttgt tattggccgt tttacttacg
     1201 gcggtttatg cattcagcca ggataaagga acattcagag ggattgtcag ggataaaacg
     1261 aacaacgaac cactgatcgg cgccaacatc atcctgaaac tggaccgttc atttggcagt
     1321 gcctccgaca tcgacggccg gttctcgctg aggctggatc ccggttccta cattttcgag
     1381 atttcttata cggggatgaa atccgattcg gtatcggtac gcatcaaccc gggtgagacc
     1441 gttgaaaggg aaatcctgct ggaacccttt gtcagccagt tgcaaggcgt agaggtcaaa
     1501 gcgggcaagt ttgaccggcc tattgaagag atgacggtgt cgatggagat tatcaaagcc
     1561 ggcttcattg aggcaaaaaa cacccagaat gtgcagacca tactcgatta cacacccggt
     1621 ttgaacattc ttgacaacga accgcagatc cggggcggca gcggattcac gttcggggtc
     1681 ggaagtaaag tagcgttact ggtagatgac atgccgatgc tctcgggcga tgccggaagg
     1741 ccttactggg acttcgtccc ggttgagaat attgagcaga tcgaagtgat caaaggagct
     1801 tcgagcgtat tgtcggggac gtcggctctg agcggtgcaa ttcatatccg gaccgcgcta
     1861 ccgaacgaca aaccgctcac aaagctgacg gtattcagcg gattgtatac cacgcctgag
     1921 gataaagaac agaaatggtg ggatgatgtc ccttacctta ccggtgcgaa ttttctgcac
     1981 acccggaaag ttgataacct cgatgtggta ttgggtggaa atgcgttttt cgatcacagt
     2041 tatatcggag caccacggac cgttcctccc gtatatgata ctaccgagaa ttttgcggat
     2101 aacaagatgg cgacgcaaaa agtacgtttc aacttcaatc tgaggaaaag gtctgaatct
     2161 taccaggggt tgaactacgg gatcaatggc aatttcatgt accagcatgc cccgatggtg
     2221 ctggcctggc ttgatgacac agccggtttt ttcaggggtt atcccggggc aaccattctg
     2281 caagaccagt tcatcggaaa tttagaccct tttattaact tttattctaa agtaggcttc
     2341 cggcacagtt ttaaagccag ggctctttac accaataacc accagtcgaa cgggcagcac
     2401 ctggaaagca ccttcatttt tgccgactat aatttcagaa gagaatacga cttcctgcag
     2461 ggcctggagt ttatcggcgg gctatcaggg caatacactc tttcaaattc cgatatctac
     2521 aaaggaagcg ggagtgaaat caacaggttc ctgaactttt ccggatatac acaatttgaa
     2581 aataacctgt tcaaaacgct tactttatcg gtaggtctga gactggaata ttacaaaatg
     2641 aatgaggcgg cttcttcatt tgagcctatt ttccgcgccg gtttcaattt aaaggtcatg
     2701 aaagaaacct atgtcagaat ttcttacgga caaggttacc gctatccgac cattgccgaa
     2761 aagttcatcc ggacggatgt cggcactttc agtgtcttcg agaacgatac acttcagccg
     2821 gaaagaagct ggaatgccga agtaggcgtc aagcaagggt ttaagttctt caattatttc
     2881 gggtacttcg attttgccgt atttcagcag gaatactgga ataccatcga atatcttttc
     2941 gggcgttggg ttgctcctac tgccgatatt tccggttatg gattcaggtt cctgaataca
     3001 ggccattcca gggtgaccgg tgtggatata tcggtcaccg gagccgtaaa cctgtttaaa
     3061 aaggtatcga tgaagaccca gatgggttat aattatattc tgcccgtatc gctgcagccc
     3121 gattttgtgt atgccagcga catatacacc acatacagtt ataacacaac cagcctggat
     3181 tcgacgcagg gtatcctgaa gtaccgcttt ctgcacacgt tcaaaggaga tatcgccttt
     3241 gatatttacg atttcacgct gggattcagc gccaaatatt tcagccggat tgaaaacctc
     3301 gacaagtcga ttgccgattt tgaaaagtat acggcttcta ccggtggaac cctgcagcaa
     3361 ataaggtata tggattattt caaccaatac aataacgggc aatggatcct ggatatcagg
     3421 gcgggttatg ccttgagtga ccataacgaa ttcagcctgc tggtcaacaa tatgctgaac
     3481 aatatatact cgctccggcc gctgaaagca gaacctatgc ggagcgtgca gctccagtat
     3541 atccacaaat tttaatatta atataccgag gatggataat gcaatcctgc tgacaaagat
     3601 cattgccacc ctgggccctt ccagttcgtc ggttgagttg gtcgggcaac tgatccggga
     3661 aggtgtgcgt gtgttcaggg tgaatttttc gcatggcagt atagattctt tctcgcggat
     3721 cattgataat gtccgccagg ccgccgcttt gtcgggtgtg ccggcggcca tccttggcga
     3781 tctgtccggg ccgaagatca ggataggcca ggtccccgat cccggaatag aattgatcaa
     3841 aggcgaaaaa gtggaattca cagcacagga tacacctgct tttcgcaaag aggacggcac
     3901 tgtagttttt tcgacgactt cgcctgagat tgtctgcgaa gtcaggaccg gtcagcggat
     3961 aatgatcgac gacgggaatg tggtcctgat atgtaccggc aaacatgaag accggctcgt
     4021 ttgcgaagtc atgcaggcag gcagggtatc ttcaaaaaaa gggatcaacc ttcccgaaac
     4081 cgacctgacc gtcgcagcaa tgacagagtg ggatgaccgg tgtgttgaat ttgccgtcgc
     4141 gaagaaagtt gattacctgg cattgagttt tgtcaggcat gcaaaagaca tcattcatct
     4201 gaaagagata ctggctgccc tgggtgccag acctttcagc ccgttcgaat accatgccga
     4261 cgacatgagc ggtgtgcccg atgatgtgca gttcatcccg atcatcagca agattgaaaa
     4321 accgcaggct atcgatgacc ttgatgggat tgtggcggag agtgatgcca ttatgatcgc
     4381 ccggggcgac ctgggcgtgg agatggagct ggcagaggtc gccgttctcc agaaaaagat
     4441 catcagtgca tgccatgacc agggtgtgcc tgtgatcgtt gcaacccaga tgctgcaatc
     4501 aatgatcgaa tctcccgttc cgacacgcgc cgaggtttct gacgtcgcca acgcgattta
     4561 cgacggagtg gatgcggtta tgctttcggg agaaacggcc gtaggcagat accctgtgga
     4621 aactgtcaga atgatggccc ggatcgcccg tttg
//
LOCUS       DAOQXS010000208         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18803_length_4653_cov_2.731187, whole genome shotgun sequence.
ACCESSION   DAOQXS010000208 DAOQXS010000000
VERSION     DAOQXS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18803_length_4653_cov_2.731187"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="PKM34_06805"
     CDS             complement(<1..2363)
                     /locus_tag="PKM34_06805"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNQ83335.1"
                     /translation="MNTKVIKFIVAQVFIAFSLAGWSLFAQEYDTITNWDGIEREWYV
                     STSGSGKAANPAPDEMNGSAHCFRFVTDEGSYEYMITELDELVDFSLCPRYRIKVLAP
                     ESGGNITLKFENYNNSFWQEIVLTPVPGQWTDLEYDFSGLVYDGLIKMVIFPDFQGNE
                     PGNYWYIDDILREACAEPEPLQLETNLPIIVINTFGEPIVDEPKIEAHMGIIDNGPGE
                     INSLDDPFNNYDGMIGIETRGQSSQMFPKKSYGFETRDAEGENLDTSLLGLPEENDWV
                     LYAPYSDKSMLRNFMSFEMGSRMGNYCTRQVFCELVINDDYKGIYTLMEKIKKDDDRV
                     DIATLNPDEISGDDLTGGYIIKVDKLDWDFEFDSDGWRSDPDPPYPNAMDIIFQYYYP
                     EADEMPSQQKIYIRDYITDAENALTSFTFSMPEIGYMQYFDVLSYVDFMLLCEISKEV
                     DKYRYSTYMYKEKDSDGGKLFAGPLWDFDLGYGNVDYWPEGLDYTGWLYYMVNPYEWS
                     IMFWWKRLMEDPYFRNMAKTRWEYLRQERLSDEYVHQMIDSVVNLLEDARVRNFERWP
                     ILGEYVWPNYDWFGNTYEDEVDYFEDFLFNRLQWMDGNFTGNLLFPWASISAQENKIS
                     LALYDDFFATRVLKKSWFTLNDAPAGVTVLTATYKDASHCELYLSEDISGAGDLSVTV
                     SHEAINYWLDLTSNRLEATGAGELPGENNSTRVYWEDQQLVVINDNPVNQPVKVEIFN
                     LAGQKMACFGIDPENVNRFSLDLKPGLYIVTLKNDRRVWKPWLQVTPA"
     gene            complement(2370..>4653)
                     /locus_tag="PKM34_06810"
     CDS             complement(2370..>4653)
                     /locus_tag="PKM34_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNQ83336.1"
                     /translation="DSLLDWCGDNQMYLILDLHGAPGGQGHDRNISDYDPDFPSLWES
                     AENRRKTAALWKRLAERYAEEPWIGGYDLINEPNWDIPGGSLLRQTYITITSAIREAD
                     TNHMIIIEGNWFANDYTGLTPPWDDNMVFSFHKYWNYNTQESIQWMINLRNTYNVPIW
                     LGETGENSNSWFADLVELAENNKIGWSWWPVKKAGINNVLMVPESPLYNQLLSFWENG
                     SPALTQQQAFEAVLDWAENHKIENCTVQRDVIDALLRQPHENSALPFRDHQVSQVINM
                     VDYDLGKCSVAYWDTDTANYHLNTGSFTDWNRGWAYRNDGVDIEPCQDDFPGTNGYGI
                     GFTAAGEWLQYTVASDTAAAYKMVIRSASESNPGSVHLTVNGIDASPSIQMPVTYGWQ
                     NWLPAIAENIIIPAGINQIRVCFDKGGSNYSLLQFSDPAPVAGVPFQFVSGTTNPDGS
                     ALIITLNKEITGFDADISNFVVMADGVPVTVNAAMVSTSSNRKLILYLADKLRFGQTV
                     TLSYSGAGIMAGDQALETFTGKPVRNTLPHRYLLPALIQAEDYIVNSGYQLENCTDIN
                     WGQNIGYANNGDYLDYYISVPQAGEYIFRLRAASLYSNGSISLRLGDGNSFTALKTIN
                     ITQTGGWQNWITIPVTIDLPQGDFTLRILSLSGEYNINWFEISLPAGENQYGPIRRFQ
                     VFPNPSDGKIRVEADFAGNSGARLKIFSSNGKILYNRDFTETFDFRQNIDLAYANPGI
                     YFLCLTTASGSSFTRKVIIN"
BASE COUNT         1127 a         1177 c         1148 g         1201 t
ORIGIN      
        1 gccggtgtta cttgaagcca tggtttccag actctacggt cattcttcag cgtcacaata
       61 taaagtcccg gctttaaatc caatgaaaac cggttaacgt tttcaggatc aattccgaaa
      121 caagccattt tctgcccggc aagattaaat atctcaacct taaccggctg gtttacagga
      181 ttatcattta taacaaccaa ttgctgatct tcccaataca ccctggtgga gttattttcg
      241 ccaggcaatt ccccggcccc ggtcgcttcg agccggttgc tggtcaggtc gagccagtaa
      301 ttgatggcct cgtgcgaaac cgtaacggaa agatcgcccg cgccgctgat atcttccgag
      361 agataaagct cgcaatggga tgcatccttg taagtggctg tcaggacagt aacaccggcg
      421 ggggcgtcgt tcagcgtaaa ccagcttttt ttcagtaccc gggtggcaaa aaagtcatcg
      481 taaagggcca ggctgatctt gttttcctgc gctgagatgc ttgcccaggg aaaaagcagg
      541 ttgccggtaa aatttccgtc catccattgt aaacggttga aaaggaaatc ttcgaaataa
      601 tcgacttcat cttcgtatgt attcccgaac cagtcgtaat ttggccatac gtactcgccc
      661 aggatcggcc accgctcaaa attccgcacc ctggcatctt ccagcaagtt aaccaccgaa
      721 tcgatcatct ggtggacata ctcatcgctg aggcgttcct ggcggagata ctcccagcgg
      781 gtttttgcca tgtttctgaa ataaggatct tccatcagcc tcttccacca gaacatgatg
      841 ctccattcgt agggatttac catgtaataa agccatccgg tatagtccaa accctccggc
      901 cagtaatcga cattgccata gcccaggtca aaatcccaga gcgggccggc aaacaatttt
      961 cccccgtcgc tgtctttctc cttgtacata tacgtgctgt aacgatattt gtcgacctct
     1021 ttggaaattt cacacagaag cataaaatca acatatgaaa gaacgtcgaa atactgcata
     1081 taacctatct caggcatgga aaaagtgaag cttgtcagcg cattctcagc atcagttata
     1141 taatctcgga tgtaaatttt ttgctgagat ggcatctcat cggcttccgg gtagtaatac
     1201 tggaagatga tgtccatcgc gttcggatag gggggatcgg gatccgatct ccagccgtcg
     1261 gaatcgaact cgaaatccca gtccagttta tcgactttta tgatataacc tcccgtcagg
     1321 tcatctcctg aaatctcgtc gggattgagg gtggcaatat caacccggtc atcgtctttc
     1381 ttgatctttt ccatcagggt ataaatccct ttgtaatcat cattgatcac cagttcgcag
     1441 aaaacctgcc gggtgcagta gttgcccatc ctgcttccca tttcgaacga catgaaattc
     1501 cggagcatcg atttgtcgct ataaggcgca tagagcaccc agtcgttctc ttcgggcaaa
     1561 ccgagcaggc tggtatcgag gttttcacct tcggcatcac gggtttcaaa accgtacgat
     1621 tttttgggga acatttgcga tgactgcccc cgggtctcaa tcccgatcat tccgtcgtaa
     1681 ttgttgaaag gatcatccag gctgttaatc tccccggggc cgttgtcaat gattcccata
     1741 tgggcttcga tctttggctc atcgactatc ggttcgccaa aagtgttgat gacgatgatc
     1801 ggaagatttg tttccagttg caacggttcg ggctctgcgc aggcctctct caggatatca
     1861 tcgatatacc agtaattccc gggttcattg ccctggaaat caggaaaaat gaccatcttg
     1921 atcaatccat cgtaaacaag acccgaaaaa tcatattcca ggtcggtcca ttgccccggg
     1981 accggcgtca gcacgatttc ctgccagaag gaattattat aattctcgaa tttcagggtg
     2041 atgttaccgc ccgattccgg cgccaggact ttgatccggt agcgtggaca caggctgaaa
     2101 tcaaccaact catccagttc ggtgatcatg tattcatagg aaccttcatc ggtcacaaac
     2161 cggaaacagt gcgccgagcc attcatttca tcaggcgcgg gattggctgc ttttccggag
     2221 ccagaggtag agacgtacca ttcccgttca atcccgtccc agtttgttat cgtatcgtat
     2281 tcctgggcaa agagtgacca tcccgcaagg gaaaaagcaa taaaaacctg tgctacaata
     2341 aatttaatca cttttgtatt cattcccgat cagtttatga tgactttgcg tgtaaaagat
     2401 gatcctgagg cggtggtcaa gcagagaaag tagatacccg gatttgcata cgccaggtcg
     2461 atattctgtc tgaaatcaaa tgtttccgta aagtcacggt tatagagtat tttgccgttg
     2521 gaggaaaata ttttcaaccg ggcaccggaa ttaccggcaa aatcagcctc aacccgtatt
     2581 ttaccgtcgg agggattagg gaaaacctga aaccgcctga tgggtccata ctggttctca
     2641 cctgccggca ggctgatctc aaaccagttg atgttgtatt cacccgataa agagagaatc
     2701 cgcagggtaa aatccccttg cggcagatca atggtcaccg ggatagtgat ccagttttgc
     2761 cagccccccg tctgggtgat attgatggtt ttgagcgctg taaaggaatt gccgtcgccc
     2821 agccggagcg aaatgcttcc attggaatag agggaagcag cccgcagccg gaagatatat
     2881 tcgcctgcct gcggcaccga gatataataa tcgagataat cgccgttatt ggcatagccg
     2941 atattctggc cccagttgat atcggtgcag ttttcaagct ggtaacctga gttgacgatg
     3001 taatcctcgg cctggatcag cgccggcagc agatagcggt gtggcagggt gtttctgacc
     3061 ggtttccccg tgaatgtttc cagcgcctgg tctccggcca ttatcccggc ccccgaatag
     3121 gagagcgtca ctgtttgccc gaaccgcagc ttatctgcga ggtagagaat cagtttccgg
     3181 ttgctggagg tgctgaccat ggcggcattg accgttaccg gcactccatc ggccatcacg
     3241 acgaaatttg aaatgtcagc atcaaagccg gtgatctctt tgttcagcgt aatgatcagg
     3301 gcagaaccat cgggatttgt cgtaccgctg acaaactgaa aggggacgcc ggccactggg
     3361 gccggatcac tgaattgtaa aaggctgtag tttgatccgc ctttatcgaa gcataccctg
     3421 atctggttga tacctgcggg aataatgata ttttcggcta ttgccggcag ccagttctgc
     3481 catccgtaag tcacaggcat ctggatggaa ggggaagcgt cgataccgtt aacagtgagg
     3541 tgtacactgc ccggattgga ttccgaggcc gagcggataa ccattttata agcagcggct
     3601 gtatcagaag ccaccgtgta ttgcagccat tcgccggcag ccgtaaaccc gataccgtaa
     3661 ccgttcgttc cgggaaagtc atcctggcac ggttcgatgt caacgccatc gttccggtag
     3721 gcccatcccc ggttccagtc ggtaaatgaa cctgtattca ggtgataatt ggctgtatcg
     3781 gtgtcccaat aagctaccga acatttcccc aggtcataat ctaccatgtt aatcacctgg
     3841 ctgacttgat gatcgcgaaa aggaagggcg ctattttcat gaggctggcg gagcaatgca
     3901 tcgattacat cacgctgtac ggtacaattt tcaattttat gattctcggc ccagtcgagg
     3961 acagcttcga aagcctgctg ctgtgtcaaa gccggggatc cgttttccca gaaggaaagc
     4021 agttgattgt agagcgggct ttcaggtacc atgagcacat tattgatccc cgctttcttc
     4081 accggccacc acgaccagcc gatcttatta ttctccgcca gttcgacgag gtcggcaaac
     4141 caggagtttg aattttcacc ggtttcgccc agccatatcg ggacattgta cgtattgcgc
     4201 aggtttatca tccattggat cgattcctgt gtattataat tccagtattt gtgaaaactg
     4261 aaaaccatat tgtcgtccca gggaggggta agcccggtat agtcattggc aaaccagttg
     4321 ccttcgatga tgatcatatg gtttgtgtcg gcttcgcgga tggcgctggt aatcgtgata
     4381 taggtctgcc ggagtagaga gcctccgggg atatcccagt tgggttcgtt gatcaggtca
     4441 taaccgccga tccagggctc ctcagcatat cgttccgcca gccgcttcca caaagctgct
     4501 gttttcctcc tgttttcagc gctttcccag agggaaggga aatcggggtc gtaatcggag
     4561 atgttgcggt cgtggccctg gccgccgggg gcgccatgca ggtcaagtat aaggtacatt
     4621 tggttatcgc cgcaccagtc aagcaggctg tcg
//
LOCUS       DAOQXS010000209         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18835_length_4646_cov_3.643215, whole genome shotgun sequence.
ACCESSION   DAOQXS010000209 DAOQXS010000000
VERSION     DAOQXS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18835_length_4646_cov_3.643215"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKM34_06815"
     CDS             <1..442
                     /locus_tag="PKM34_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ83337.1"
                     /translation="GISLYGIATLEEEQPRLQPVGRLKSAISQIKTVAAGESIGYNRA
                     FRAKKQIRMAIVPIGYADGLSLALSNGKGHLMVGGKRVPVIGRVCMDMCMLDITGIEA
                     EEGDEVIIFGPERPLQELAKEMGTIPYEVLAGLSRRVKRVYYHE"
     gene            500..1252
                     /locus_tag="PKM34_06820"
     CDS             500..1252
                     /locus_tag="PKM34_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83338.1"
                     /translation="MLIRSSMEIKRLSIIVPAYNEARTIHLILDKVLAVRLINDIEKE
                     IIIVNDCSKDETEKVVNQYIEEHKEANVRLFNQPVNMGKGAALHRGIQEASGEYLIIQ
                     DADLEYDPEEYNDLLKPVVNGFADVVYGSRFMGSKPHRILFFWHTIGNKFLTFISNMF
                     TNLNLTDMETCYKLFKSDYIKKIYFKENRFGFEPEVTAKISRFPKIRIYEVGISYYGR
                     TYEEGKKIGARDGFRAIWCILKYNLFSKRYLK"
     gene            1286..2755
                     /locus_tag="PKM34_06825"
     CDS             1286..2755
                     /locus_tag="PKM34_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83339.1"
                     /translation="MARQFNNYLTGIRDRMTGQQSILFLINSALFIAGILLLIGPAIQ
                     NGFPILHADSGTYLLEGFGTKIPVSRPLSYCLFVRFTSRIFSIWSVVVTQAIIAYWTI
                     WLAVNTLAGKKKIAWLPFITVALLSVTTGISFYVSQIMTDIFMPVALLGLFILVVRER
                     MPWYTWLFLGLIVWLSLIVHMSNLPVLTGLAFAIIILKIILEKSIKPVKQLSVYIIAG
                     ILVISWLTNPLISTIYGEGFRTSNSGNIVFFSRLLQAGAAQKYIKDRCEKEPEYYLCE
                     YKDQIDTYDRLDKFLWPDTSFLYDHPCREKTWDYCWRVRNQEFGEINSAILDHPPSRN
                     IYLKAVWDDFQLALHAFGLTGYVSFREGSHIDYPLKRYYKNDYKSFKNSRQYAGTMVF
                     RDMNRIIHVVVWISLALAAFLIIRNRRILDRRSPYLILMSILVLTWLGNALLTAFVAV
                     VSERFLGRFIWLLPFFVIMLVYREYSALYADSADKAREE"
     gene            complement(2766..3734)
                     /locus_tag="PKM34_06830"
     CDS             complement(2766..3734)
                     /locus_tag="PKM34_06830"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ83340.1"
                     /translation="MTTYTLVTGANGYLGSYICRELISRGMPVIALRFDHYASVILEH
                     PLIEYVYCDITQPVLPQLESAIKGKKINTIINAAALLGSSDYEKNRKVNAGGVMNLIE
                     FAKSQGIKRFIQISSVVVIKTFKGPYGETKLIGQQYVEQSGLDYTVFIPAMILGPEGL
                     GINRILKNVFRFPLFVPLIGSGKQTQHPIFVRDFAKYIVLAIDNPSAIGKTYQIASDR
                     VISFRDLIRLVLKIKGKRRIFVSFPAPFARLLGKFFQKTQKVPLFTAEHVKGVLQDSN
                     LDTSLIRQDLGDHFTRLEDAMKYTLDEIGGNWDFYLKTRSEKVVKL"
     gene            complement(3791..>4646)
                     /locus_tag="PKM34_06835"
     CDS             complement(3791..>4646)
                     /locus_tag="PKM34_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ83341.1"
                     /translation="LGELWSWIRTVDGTSLFLAILFQLLVLLIKAWRWYNLNEAGFDA
                     RRLGRRSGEFFEGYAMGVITPGRMGELMKAGHAGTRTGILGTGLRVIAERGMDLSIFI
                     VVAGIAMSQGALPGVSNLWGWLVLAAGILGMSLSLIILISPGMVLFAEYTMKMVRMLP
                     KDKTLEFVSRTPWNSAGFVAWSLMSNLSFFVCCYFLAAGVGIGLDIIHVSGGVAVAGV
                     LNSIPVTVMGLGTREVTFLYVFSAFPRAQVMAFSGLVFLVAQIGGGIISLVMGQILLF
                     KYKANQVQ"
BASE COUNT         1159 a         1126 c         1144 g         1217 t
ORIGIN      
        1 ggggatcagc ctgtacggga ttgccacgct ggaggaggag caaccccggc tgcaacccgt
       61 gggcaggctg aaatcggcta tttcgcagat taaaacggtg gcggccgggg aaagcatcgg
      121 ctacaaccgt gctttccggg caaaaaagca gatccggatg gcaatcgtcc cgatcggtta
      181 tgccgacggg ctaagcctgg cgctgagcaa cggaaagggg cacctgatgg ttggcggcaa
      241 acgggtgccg gttatcggcc gggtgtgcat ggacatgtgc atgctggata tcaccgggat
      301 cgaagcggaa gagggcgatg aggtgatcat tttcggccct gaacggccgc tgcaggagct
      361 ggcaaaggag atgggcacca tcccgtatga ggtgctggcc ggtctgtcga gacgggtgaa
      421 acgggtttat tatcatgaat agcgcatttt atttcccatc aaacggaatt tttatttctt
      481 ttgatgaaga ttcggtttta tgctcataag atcatccatg gaaatcaaaa gattatcgat
      541 tatcgtccct gcttacaatg aagcccggac cattcacctg atccttgaca aggttctcgc
      601 cgtcaggctt atcaatgata ttgaaaagga gatcatcatt gtgaatgatt gttcgaaaga
      661 tgaaacggag aaggttgtaa accaatatat tgaagagcat aaagaggcca atgtccggtt
      721 attcaaccag cccgtcaata tgggaaaagg ggcagcgctt caccggggca ttcaggaagc
      781 gtctggtgag tacctgatca tccaggatgc cgacctggaa tatgatccgg aagagtacaa
      841 cgacctgctc aagccggtcg tcaatggatt tgccgatgtg gtttacggct ccaggttcat
      901 ggggagcaag ccccaccgca tcctgttttt ctggcatacc atcggtaata aattcctcac
      961 cttcatctcc aacatgttca ctaatcttaa tcttacagat atggaaactt gttataagct
     1021 tttcaaatct gattatatca agaagatcta ttttaaggaa aaccggttcg gatttgaacc
     1081 tgaagttaca gccaagattt cacgctttcc aaagatcagg atttatgagg tcggcatctc
     1141 ttattatggc aggacctatg aagagggaaa gaaaatagga gcccgtgacg gtttccgggc
     1201 tatttggtgc atccttaaat ataatttgtt cagtaagaga tacctgaagt aaagaagttc
     1261 cggaaatttt caacagaagt tgctcatggc cagacaattc aataactatt tgaccggaat
     1321 ccgggaccgt atgaccggtc agcaatccat cctgttcctg atcaattcag ccctctttat
     1381 agccggtatt ttgctcctga tcggaccggc cattcagaac ggattcccga tacttcatgc
     1441 cgactcgggt acctacctcc tggaaggatt tgggaccaag atccccgtca gccgcccgct
     1501 ttcatactgc ctgttcgtca ggtttacctc caggatcttt tcaatctggt ctgtcgtcgt
     1561 cacgcaggcc atcattgcat actggaccat ctggctggct gtaaatacgc tggcaggcaa
     1621 gaaaaagatc gcctggctcc ctttcattac cgttgccctt ttgagtgtca cgactggaat
     1681 ttccttctat gtcagccaga tcatgaccga tattttcatg ccggtagccc tgctgggttt
     1741 gtttatcctg gtcgtcaggg aaaggatgcc ctggtacaca tggctgttcc ttgggctaat
     1801 cgtctggtta tcattgattg tacatatgtc taatcttccg gtattgacag ggctggcgtt
     1861 tgcaatcatc attttaaaaa taatactgga gaaatcgatc aagccggtaa agcaattgtc
     1921 agtgtatatt attgcaggca ttctggttat ttcatggctt acgaatccgc tgatcagtac
     1981 gatttatggc gaagggttca ggacgtcgaa cagcgggaat atcgtttttt tctcccggtt
     2041 gttgcaggca ggggcagcgc agaaatacat caaagaccgt tgtgaaaagg aaccggagta
     2101 ttacctgtgc gaatacaagg atcagattga cacatatgac cggctggata agtttttatg
     2161 gcccgatacc agttttcttt acgaccaccc ctgtcgcgag aaaacctggg actattgctg
     2221 gagggtgcgt aaccaggaat ttggcgaaat aaattcagca attctggatc atccgccaag
     2281 ccggaatatt tatctcaagg ctgtatggga tgattttcaa ctggctttgc atgctttcgg
     2341 gcttacggga tatgtttcat tccgggaggg atcgcatatc gattatcctt taaagcgtta
     2401 ttacaagaat gattacaaat cctttaagaa ttcacggcag tatgccggga caatggtatt
     2461 ccgcgatatg aacaggatca tacatgttgt tgtatggatt tcgctggctc tggcagcttt
     2521 tctgataatc cggaaccgga gaatcctgga tcgtcgttcc ccttacctga tacttatgag
     2581 catcctggtc ctcacctggc ttggaaatgc cctgctcacc gcttttgtcg ctgttgtttc
     2641 ggaaaggttc ctgggccgct ttatatggct cttgccattc tttgtcatca tgctggttta
     2701 ccgagaatac agcgcattat atgcagactc tgccgataag gcacgggaag aataagctga
     2761 ccagattata gcttcactac tttttcgctc ctggttttca ggtagaaatc ccaattcccg
     2821 cctatttcat ccagggtgta tttcattgca tcttccaggc gggtgaaatg atccccaaga
     2881 tcctggcgga taagggaggt gtcgaggttg gagtcctgga ggaccccttt gacatgttct
     2941 gcggtgaaca ggggaacctt ttgcgttttc tggaagaatt tacccaacaa cctggcaaaa
     3001 ggagcaggga agctgacaaa gatccttctt ttccccttga ttttcagtac aagccggatc
     3061 agatcgcgga aagagatgac cctgtcgctg gcaatctggt atgtttttcc gatggcagag
     3121 gggttatcga tggcgagtac gatatatttt gcaaaatccc tgacgaagat cgggtgctgc
     3181 gtttgtttac cgcttccgat caacgggacg aataaaggaa agcggaatac gttcttaagg
     3241 atccggttga tgcccagccc ttccggcccg aggatcatag cgggaatgaa taccgtgtaa
     3301 tcaagtcctg attgctcaac gtattgctga ccgatcagct tggtttcgcc ataaggccct
     3361 ttgaatgttt ttatcacgac cacgctgctg atctggataa accttttgat cccctgcgat
     3421 ttcgcgaatt cgatgagatt catcacacct ccggcattca ccttccggtt cttttcataa
     3481 tctgaactcc ccagcaatgc ggccgcattg atgattgtat ttattttttt tcctttgatg
     3541 gcagattcaa gctgggggag taccggttgg gtaatgtcgc aataaacata ttcgatcagc
     3601 ggatgctcca ggatgaccga agcataatga tcgaaccgca gcgcgatgac gggcattccc
     3661 ctgctgatca gttccctgca tatgtagctt cccaggtatc cgttggctcc cgtaacgagg
     3721 gtgtaggtag tcataagtcg ttagtcgtta atttatgtcc ggctttaaaa gattattcgt
     3781 tattcgttat tcactggact tgatttgcct tatatttgaa caggaggatt tgtcccatca
     3841 ccagtgagat gataccgccg ccaatctggg ctaccaggaa gaccaggccg gagaaggcca
     3901 tgacctgggc ccgcggaaaa gctgaaaaca catacagaaa ggttacctcc ctggttccca
     3961 gccccataac ggtgaccggt atggaattca acacgcctgc cacggcaact ccccctgaaa
     4021 catggataat gtcgaggccg atccctactc cggcagccag gaaataacag catacaaaga
     4081 aactcaggtt gctcatcagc gaccaggcta caaatcctgc agaattccag ggcgttctgc
     4141 tgacaaactc cagcgtttta tcttttggca gcatccggac cattttcatg gtatattcag
     4201 caaaaagtac cattcccggg gaaatgagaa tgatcagcga caggctcatg ccgaggattc
     4261 cggccgcgag caccagccag ccccacaaat tactgacccc gggcagggcg ccctgcgaca
     4321 tcgcaattcc ggcaacgacg atgaatatgc tgagatccat ccccctttcg gcaataacgc
     4381 gtaaaccggt gccaagtatc ccggtccgtg ttccggcatg tccggctttc atcagctctc
     4441 ccatcctacc gggcgtgatc acccccatgg catagccttc aaaaaattcc cccgatctcc
     4501 ttcccaggcg acgggcatcg aatcccgcct cgttcaggtt gtaccatcgc caggctttga
     4561 tcagcagaac cagcaattga aaaagtatgg caagaaacag acttgttcca tcaaccgtcc
     4621 tgatccagct ccataattca cccagg
//
LOCUS       DAOQXS010000210         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_18980_length_4619_cov_3.671998, whole genome shotgun sequence.
ACCESSION   DAOQXS010000210 DAOQXS010000000
VERSION     DAOQXS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18980_length_4619_cov_3.671998"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PKM34_06840"
     CDS             <1..1386
                     /locus_tag="PKM34_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83342.1"
                     /translation="GLIFSKRENKGTGDWTNSYLSPVSPISPTWPRIVTTGEQNEILH
                     VIYDSYAAYNDQTYALLYARSSDGGETWDVLNQTFEDFGANYYSEIGGDAYGIAANGN
                     TVAVLISDTWTSDLAYVKSTDGGETWDKTVIWEHPYPFYDWDVTVTDTFFAPDGSATI
                     AVDNNGKVHVSFGICRVLHDVVGTNYTLWPGCWAVGYWNEDMEPFSLGNNTLAPAEWE
                     KEGSELIDEYNRIGYVLETDPADLKDEYMYYRTIGLVNMPAITVDDLGRVFVVYSAIA
                     DGYMNATYNYRHIWARGYENGIWGDFVDLNTDIFHIFDECIWPVVSPTSDDYIHIMYM
                     ADQTPGTALDSDHSYVINNMYYAALPKEDLLTGVKDVEPVTIESVSQNFPNPFNGMTT
                     VAVKLTSKANLSLTVTNMLGQEVLSIEKGEVPEGVQYFEIDAANLTNGVYFYTVRAGE
                     ATMTRKMIVRK"
     assembly_gap    1472..1571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1830..3689
                     /locus_tag="PKM34_06845"
     CDS             1830..3689
                     /locus_tag="PKM34_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83343.1"
                     /translation="MKKILLLLVLMIACTAAFSQKAAKLTREMKELNFKATRPSSPSD
                     EILFGNVSDQFVRNPITSPVESVIGDTKYDLQSNRMLQGRIHMFPDGSVGAVWTRGLT
                     ETAFPDRGTGYNYFDGAEWGPAPTERIESARTGWPAYAPWGPNGEIVVSHDFTNGLYI
                     LTRENKGQGDWQESTLGSVAPVVPSWPRIATGGENNDIIHMVYNSKNAAYDQVTALLY
                     TRSFDGGLNWDPELFFDDLGPNYYSEIGGDSYAITAKGSTVAILIADTWKSDLAILKS
                     TDYGDTWDKTIIWEHPYPFYDWDATITDTFASVDGSAGIAIDNNGKVHVVFGLCRVLH
                     EAVGNSYNYWPYAAGIGYWNEDMPTFSNNVNALLPPEWEYEGTELIDEYNRIGYLLDT
                     DPNNEFKDEFWLYRTIGLLSMPTLSIDDNNNIFLVYSGVADGYFNATYNYRHIWARGY
                     ANNIWGDDIVDLNTDIIHIFDECVFPVVSPTSNEDIHVMYQTDQIPGLALDSDHAWVV
                     NSMYYAALPKEDLLTGIADPRASETLTQVSQNFPNPFSGKTYIDVKLESDAGLSVSVT
                     NTLGQEMLNVNKGIVRAGLYRFEIDGSNWENGIYFYTLVTGSQRVTKKMILHR"
     gene            3760..>4619
                     /locus_tag="PKM34_06850"
     CDS             3760..>4619
                     /locus_tag="PKM34_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83344.1"
                     /translation="MKKLLLIVLLMSFLAVNAQYNRSEMRKISPHAPKAVYYENTGSS
                     PAAFDASQLTDNVATPFSGTRDVKSVTPIQIGTSGNAFGFAFHRTAYLWADNNINSIS
                     FIHRMTATPGTGYLAYDLSKAGGAAGTWTNNIQAYSAAPPSSNARYPQGGLFNPPGNT
                     DPDNAYFHYFAPTLDGSNTSGTLNWGGYAYGVESLADGSTGTKTNRASSDPYYQFLPS
                     GFTITQSGEAWMVDESTMGIGNDEYEYQGSLIVGHATWNPDISDMDYLFDLFPLEIGA
                     EAGFNDIKIA"
BASE COUNT         1176 a         1134 c         1067 g         1142 t
ORIGIN      
        1 ggacttatct tcagcaaaag ggaaaacaag ggaaccggcg actggacgaa cagttacctg
       61 tcacctgtat ccccgatcag ccccacctgg ccgcgtatcg tcacaaccgg cgaacaaaac
      121 gagatcctgc atgtgatcta tgattcgtat gctgcataca atgatcagac ctatgccctt
      181 ctttacgcac gttcgagcga cggtggcgaa acttgggatg tcctgaacca gacctttgaa
      241 gatttcggcg ctaactacta ttcagaaatc ggcggcgacg cttatggaat tgcagccaat
      301 ggaaacacgg tggccgtcct gatatccgac acctggacca gcgacctggc ttacgtcaag
      361 tcaaccgacg gcggtgaaac ctgggacaaa accgttatct gggaacatcc atatcctttc
      421 tatgattggg atgtaaccgt taccgatact ttctttgcgc cggacggttc tgcaaccatc
      481 gctgttgaca acaacggtaa agttcatgta tccttcggta tctgccgcgt tctccacgat
      541 gtggtcggaa ccaactacac cctgtggccg ggctgctggg cagtcggtta ctggaatgag
      601 gatatggaac cgttcagcct cggcaacaac acccttgccc cggcagaatg ggaaaaagaa
      661 ggatcagaac tgatcgacga gtacaaccgt atcggttatg tcctcgaaac tgaccctgcg
      721 gacctgaaag atgagtatat gtattacagg actattggcc ttgttaatat gcccgctatc
      781 actgttgacg atctcggcag ggtattcgtg gtttactcgg ctatcgctga cggatatatg
      841 aatgccactt ataactacag gcacatctgg gcccgcggtt atgaaaacgg catctggggc
      901 gactttgtcg atctcaacac cgatatcttc cacattttcg acgaatgtat ctggccggtc
      961 gtttcaccga catccgatga ctatatccat attatgtaca tggccgacca gaccccgggt
     1021 accgccctgg attcggatca ctcctatgtt atcaataata tgtattatgc tgctcttccc
     1081 aaggaagacc tgctgaccgg cgtgaaagat gtggaaccgg taactatcga gagtgtttca
     1141 caaaacttcc ccaacccgtt caacggcatg acgaccgtcg ccgttaaact gaccagcaaa
     1201 gccaacctca gcctgaccgt taccaatatg ctcggacagg aagtgttgag catcgaaaaa
     1261 ggcgaagttc cggaaggagt tcaatatttc gagattgatg ccgccaacct caccaatggt
     1321 gtttatttct acaccgttag ggcaggtgaa gctacgatga cacggaaaat gatcgtccgc
     1381 aaataatatt ttctctttaa taaacaagaa ggctgctcag atacccgggc agcctttttt
     1441 tttattccgt cgggtcctgt gttttcttcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nacgactaaa gccgtcggtt cttgtgtttt cttccgacga ctaaagccgt
     1621 cggttcttgt gttttcttcc gacgactaaa gccgtcggtt caattattat catccatttg
     1681 ttaaccggaa aaggcagtat ggaagttaat cccctccatc ttttctgaaa atatcttaaa
     1741 attaatggct taaaagatgt attgtaaatt agtttatatt tatctcatgt tttattcgaa
     1801 ttctttagta caattaaatc ttacctgaaa tgaaaaagat tctactactg ttggttctta
     1861 tgatcgcctg cactgctgca ttttcccaga aagcggcgaa acttacccgg gagatgaaag
     1921 agctcaactt caaagctacc cggccatcct ctccatcgga tgaaattctt tttggcaatg
     1981 tatccgatca gtttgtcaga aacccgataa cctcgccggt tgaatctgtg atcggagata
     2041 ctaaatatga tttgcagtcg aaccggatgc tacaggggcg tatccatatg tttccggacg
     2101 gatcagttgg ggcggtctgg acccggggac tgaccgaaac ggctttcccg gacaggggaa
     2161 cgggatacaa ctactttgac ggcgcggaat ggggcccggc accgacagaa cgcatcgaga
     2221 gcgcccggac cggctggccg gcgtatgcac cctggggccc gaacggcgag atcgttgttt
     2281 ctcacgactt taccaatgga ttgtatatcc tcacaaggga aaacaagggc cagggcgact
     2341 ggcaggaatc caccctcgga tccgtagccc cggttgtgcc cagctggccc aggattgcca
     2401 ccggaggaga aaacaatgac attatccata tggtttataa ctcaaagaat gccgcttacg
     2461 accaggttac ggctctgctt tacacccgtt ccttcgatgg cgggctgaac tgggacccgg
     2521 agttgttctt tgatgatctg ggccctaatt attattccga gatagggggt gactcgtatg
     2581 ctatcactgc aaaaggcagt acggtagcca tcctgattgc cgacacctgg aaatccgatc
     2641 tggccatttt gaaatcaacc gattacggag atacgtggga taaaaccatc atctgggagc
     2701 acccttatcc gttttatgac tgggatgcaa ccattaccga tacttttgca tcggtggatg
     2761 gatcagctgg aattgccatc gataataacg ggaaagtcca tgtggtattc ggcctctgcc
     2821 gtgtattgca cgaagctgtg ggtaattcct ataattattg gccctatgct gccggtatcg
     2881 gttactggaa tgaagatatg ccgactttca gcaacaatgt caatgctctc cttccgcccg
     2941 aatgggaata tgaagggacc gaactgatcg atgaatacaa caggataggc tatcttctcg
     3001 ataccgatcc gaacaatgaa ttcaaggatg agttctggct ttaccggacc attggacttc
     3061 tgtcgatgcc aacccttagt atagacgata acaacaacat cttcctggtc tattccggtg
     3121 tggccgatgg atatttcaat gccacctata actacaggca tatctgggca cgcggatatg
     3181 ccaacaatat ttggggggat gacatcgttg acctgaatac cgacatcatc cacatttttg
     3241 atgaatgcgt ttttccggtg gtttccccga ccagcaatga agatatccac gtcatgtacc
     3301 agactgacca gatccccggc ctggcattgg actccgatca tgcctgggtt gtcaatagta
     3361 tgtattatgc ggcacttcct aaagaagacc tccttaccgg tatcgctgat ccacgggcaa
     3421 gtgagacact tacgcaagta tcgcaaaact ttcccaatcc attcagcggg aaaacttata
     3481 ttgatgtaaa gctggaatct gatgccggat tatcggtctc tgtaaccaat acccttggcc
     3541 aggagatgct gaatgtcaat aaaggtattg tcagggccgg tttataccgt tttgagattg
     3601 atggcagcaa ctgggaaaat ggaatctatt tttatacatt ggtaacggga agccagaggg
     3661 tgacgaaaaa aatgatcctg caccggtaat ggttctttaa tcaaaaaatg ataattttat
     3721 aggcaataat taaacatgta taactaaaca ttcttttcca tgaaaaaact tttactgatt
     3781 gttctgctaa tgagcttcct ggctgtcaat gcacaataca acaggagcga aatgaggaaa
     3841 ataagccctc atgcccccaa ggctgtatat tacgaaaaca ccggttcgtc gccggctgct
     3901 tttgatgcat cacaactgac ggacaatgtg gcaactccat tcagcggtac ccgtgatgtc
     3961 aaaagcgtaa ctcccatcca gatcggtacg tcgggcaatg cattcggttt tgcctttcac
     4021 cggacagcct atctttgggc agataataac atcaattcca tatcattcat ccaccggatg
     4081 accgccacgc cgggtacggg ctacctcgcc tacgacctgt cgaaagcagg cggagctgcc
     4141 ggaacctgga ccaacaatat ccaggcgtac agcgcagccc ctccttcttc gaatgcgcgc
     4201 tatccgcagg gaggcctctt caatcctccg ggcaacaccg atccggataa tgcttacttc
     4261 cactactttg cacctaccct cgacggttcc aataccagcg gaacccttaa ttggggtgga
     4321 tatgcctacg gcgtggagag cctggccgat ggttcgaccg gcactaaaac caacagggcc
     4381 tcttcagatc cttattacca gttcctcccg tcaggattta ccatcactca gtcgggcgaa
     4441 gcctggatgg tagatgaaag tactatgggt atcggtaatg atgaatacga ataccaggga
     4501 agcctgatcg tcggccatgc cacctggaac cccgatatca gcgatatgga ttaccttttc
     4561 gacctgttcc cgctggaaat tggcgccgaa gcaggattca acgatatcaa gatcgcttt
//
LOCUS       DAOQXS010000211         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19070_length_4602_cov_3.597537, whole genome shotgun sequence.
ACCESSION   DAOQXS010000211 DAOQXS010000000
VERSION     DAOQXS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19070_length_4602_cov_3.597537"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4602
                     /locus_tag="PKM34_06855"
     CDS             <1..>4602
                     /locus_tag="PKM34_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83345.1"
                     /translation="PLCGNYTAGASGADFTTVGQAVTLLNLSGITCPVTIFLKDGIYY
                     EKLLIKDIPGASETNTVTFTSQSGDSSKATIKIPGNALKDEDMIYMEETRHVTFRDLG
                     LFTGTTPSTSFSNNAVNLNNTRYITFENCYFEVKKESDIGISIQPGCEAIYIRNSRFD
                     NLNPKAGAIAAKGDQTRNIEIAGNTIKGTTEWQYSAISFSNNVDNIRINDNLIENCFR
                     GIYVVSCDTIQVMGNIIKNTNEGVNIYNCFAVSEVSGNRLIDIINHPNASDGTSAIMV
                     NTASKVKVYNNYIVTTGTGPVIGINLQAASQCSVDFNSLNIINADAQGKGKGINVRNS
                     SNISARNNIVSISNPGYPVYFQESTNQIVFDYNDYYSLEKRIGYFNTAGYNDLQSWRG
                     ATGFDQHSVAANPFYTSVTDLAINQALLNNSGIPVAGITADIDGNSRNVTTPDIGAHE
                     YSPCVVDAGINAITSPESPLSGGNEEIRVILQNQGTSMLSSVKIYWAVNEIQQGYYTW
                     NGSLGLKANTEVMLGNYNFVAGQSYLVKAWTSEPNNTSDCNNKNDTISSGELSGPLCG
                     TYTIGGSDGDFATFSQVAEMLNSAGITCPVTFLVRDGIYNEKFILKQVTGSSETNTIT
                     FASESGDSSMAILRLETGSTNYDPVIKLEGSQYLRFTGIKIVTESTSGNNYGILFEGA
                     DNITIENCFIESKRDADAGLLIRGGSRQVQVIRTHFECTHSKAAAIDISGQQTRDISI
                     NQCTINGAPEINEVYGNVLVKSGTSVKNIEIGENLIRNSNTAITCSGTDSIRLTKNII
                     QNSHYGIVANNECKHINISGNRLLRVQSVLSAPDGVSGIFAENIDSIDIINNYIQSTG
                     QGPVLGINIQNSNNCRVSYNSVNITNTDAQGKSKGINLRNTDGVTGRNNIFYIKSSGI
                     PIHVDQNVTNLALDYNNYYNPAGIIGKLLNQVFNNLSQWGQSISGDANSLAVHPYFKS
                     DTIPLPYQRILNGSGIPVAGIRYDIDGKLRHIQAPDMGCMEFFIDFGVLEMISPNLEC
                     FHEDLEPVIVYIRQFGDVPFDNLIIRYQLNDGDIHTDTIQGPLEFDFIHEFDETETLE
                     TYGDYLFKIWLVNNADDNINNDLLQVWRYSKPSPIISIDYNNACTGNEVYFTANATVD
                     SPYYIEKYEWIFGDGDTAYTQNPVHLFDEAGSYPVVCRAYSNAGCYNDTSFVITLDPA
                     YELLSISYDLDDETCHNSGTGTLQMSAVGGTPPYTFYLNGSAVPDGFIASLASGKYQL
                     SVEDAAGCTAVDSAEARTLIFMDPEIIASQLTGNAPFNVNFDYEADGATGCTWHFPGN
                     ITDTLKNATFTFTEYGDYTIVLEVNSGEPFYCIETDTVNIHVDIIVVIEPNSVFTPNG
                     DGHNDYFEVKMYGLETMNVKIFNQWGNRIYEITDVEGKWDGNTDDGVEAADGTYFYNL
                     TARGVDGLEYSRQGTVMLLRHGAVAFPNPVLNSVSIKTFGPLDPPVYIEIFSVFGEKV
                     YSGLETQPGNIHLDLAELSKGIYFIRVHDNRKEYYVRII"
BASE COUNT         1320 a         1060 c         1086 g         1136 t
ORIGIN      
        1 ttccgctatg cggcaattat accgcaggag cttcgggtgc agattttaca acggtaggtc
       61 aggctgtcac gcttctgaat ctttcgggga ttacctgtcc ggtaactatt ttcctgaaag
      121 atggaatata ctatgaaaaa ctgctgatca aagatatacc cggagcctct gaaacaaata
      181 cggtcacttt cacttcacaa agtggagaca gttcaaaagc caccattaaa atacccggga
      241 atgcactaaa ggacgaggat atgatctaca tggaagagac ccgccacgtc actttccgtg
      301 atctgggcct tttcaccggg accactccat ccacgagctt ctcgaataat gccgtcaatc
      361 ttaataacac gcgatatata acctttgaga attgctattt tgaagtgaaa aaggaatcgg
      421 atatcggtat ttccattcag ccgggatgtg aagccattta catcaggaac agccggttcg
      481 acaacctgaa tccgaaggcg ggcgcaatag cagcaaaggg cgaccagaca agaaatatag
      541 aaattgccgg aaacaccatc aaaggcacaa cagaatggca gtattcggcc atttcgttca
      601 gcaacaatgt ggacaacatc cggatcaacg acaatctcat tgaaaattgc ttccgcggca
      661 tatacgtagt cagttgcgac accatccagg taatgggaaa tattattaaa aacacgaacg
      721 aaggggtcaa tatttataat tgttttgctg tttcggaagt atccggaaac cgccttatcg
      781 atattattaa tcatcccaat gcctccgacg gtacaagcgc tattatggtg aacaccgcat
      841 caaaagtaaa ggtttacaat aattatatag taacaacggg gaccggcccg gtgatcggaa
      901 tcaacctgca agcggcatct caatgttctg ttgatttcaa tagtttgaat atcattaatg
      961 cagacgcgca aggtaaagga aagggcatca atgtcaggaa cagtagcaat atcagcgccc
     1021 gtaataacat tgtcagcata tccaatccgg gatatccggt atatttccag gagagcacca
     1081 accagatagt tttcgattat aacgattatt acagcctgga aaaacggatc gggtatttca
     1141 acaccgccgg ttataacgat cttcagtcgt ggagaggggc cacaggattt gaccagcatt
     1201 cggttgcagc caatccgttc tacacatctg taacggatct tgcgatcaac caggctctgt
     1261 taaataactc cggtattccc gtggctggaa tcacggctga tattgatggc aacagcagga
     1321 atgttacaac tccggatatt ggcgcacacg aatacagccc atgtgtcgtt gatgccggga
     1381 tcaatgcaat tacaagcccg gaatccccgc tgagcggagg taatgaggaa atccgggtta
     1441 tattacagaa ccaggggaca tccatgcttt catcggttaa aatctattgg gcggtaaacg
     1501 aaatccagca gggatattat acctggaacg gcagtctcgg gttaaaagca aacacagaag
     1561 tcatgctggg caattataac tttgttgccg gacaatcata tcttgttaag gcatggacat
     1621 cggagcccaa caacacttcc gattgtaata ataaaaacga tacgatttcg agcggagaac
     1681 tatcaggtcc tttatgcggt acttatacaa ttggtggttc cgacggcgat tttgccactt
     1741 tcagccaggt agccgaaatg ctcaattctg cgggaataac ctgccctgtg actttcctgg
     1801 tgcgggacgg catctacaac gaaaagttca ttttaaagca ggtcacgggt tcatctgaaa
     1861 caaataccat cacattcgca tctgaaagcg gcgacagctc catggccatc ttacgtcttg
     1921 aaaccggctc aaccaactac gatcctgtca tcaaactgga aggatcacaa tatttgcggt
     1981 ttaccggcat caagatcgtc actgaatcca catcgggcaa taactacgga attctctttg
     2041 aaggcgcgga taatatcacg atcgaaaatt gcttcatcga atcaaaacgc gacgcggatg
     2101 ctggcctgct catcagggga ggcagtcgtc aagtccaggt gatcagaacc catttcgagt
     2161 gcactcattc aaaggctgct gccattgaca tctcagggca gcaaacccgg gatatcagta
     2221 taaatcaatg taccatcaac ggggcacctg agattaacga agtatacggg aatgtgctgg
     2281 tcaaatcagg tacctcggta aaaaatatcg aaatcgggga aaacctcatc aggaatagca
     2341 atacggccat cacctgttcc gggactgata gtatccgttt gactaaaaac attattcaaa
     2401 actcacacta cggaattgtt gccaataacg aatgtaagca cataaacata agcggtaaca
     2461 ggctgctcag ggtgcaaagc gtgctgagcg caccggacgg agtcagcggg attttcgcag
     2521 agaatatcga ttcgatagac atcattaaca actatatcca gtcgactggc caggggccgg
     2581 tactgggtat caatatccag aacagcaata actgccgtgt gtcttacaac agcgtcaata
     2641 ttaccaatac cgatgcgcag ggtaaaagta aagggataaa cctgagaaac accgacggcg
     2701 tgacgggcag gaataatatc ttttacataa aatcctccgg gattccgatt catgtcgacc
     2761 agaatgttac caacctggcg ctcgactata ataactatta caatccggcc ggaatcatcg
     2821 gaaagctgtt gaaccaggta ttcaataacc tttcgcaatg ggggcaaagc atttcaggag
     2881 atgccaattc actggcggtg catccctatt tcaaatccga taccattcct ctgccctatc
     2941 agcggatttt gaacggttcg ggcattccgg tagcggggat caggtatgat atcgacggca
     3001 agctgaggca cattcaggcg cctgacatgg gttgtatgga gtttttcata gatttcggtg
     3061 tgctggagat gatcagcccc aacctggagt gcttccatga ggacctggaa ccggtcattg
     3121 tatatatccg ccagttcggc gacgtaccgt tcgataacct catcatcagg taccagttga
     3181 atgatggcga catccacacc gatacgatcc aggggccact ggaattcgat tttatccatg
     3241 agttcgacga aaccgaaact ttggaaactt acggggacta cctttttaaa atttggctgg
     3301 tgaacaatgc agatgataat atcaacaacg atttgcttca ggtatggcgc tattcaaagc
     3361 catctcccat tatatcaata gattataaca atgcctgtac aggtaatgaa gtctacttta
     3421 ccgcgaatgc aactgttgat tccccctatt atatagaaaa gtatgaatgg attttcgggg
     3481 atggtgatac tgcatacacc cagaacccgg tccacctgtt tgatgaagcc ggatcgtatc
     3541 cggttgtatg ccgggcttac agcaatgccg gttgctacaa cgatacgagt tttgtcatca
     3601 ctcttgatcc tgcgtatgaa cttctctcca tttcgtatga cctggatgat gagacctgtc
     3661 ataacagcgg cactggcacc cttcagatgt cggcggtggg aggcacgccg ccttacacct
     3721 tctacctgaa tggcagcgcg gttccggatg ggtttatcgc cagcctggct tcgggcaaat
     3781 atcagctgag tgtggaagat gctgccggtt gtacagccgt ggattcagcg gaagcccgta
     3841 ccctgatctt catggatcct gaaatcattg ccagtcagct gacaggtaat gcccctttca
     3901 acgtcaactt tgattatgaa gctgacgggg caacaggctg tacctggcat tttcccggaa
     3961 atatcacgga taccctcaaa aacgcaactt tcacttttac tgaatacggc gattacacga
     4021 tcgtgctgga ggttaattcg ggtgaacctt tctattgcat tgaaactgat acggtgaata
     4081 ttcatgtcga tattattgtc gtcatcgagc ccaattcggt atttacacct aacggggatg
     4141 ggcataatga ctatttcgaa gtgaagatgt atggtttgga gacgatgaat gtaaaaatct
     4201 tcaaccaatg gggaaacagg atttatgaga tcacggatgt ggaaggcaaa tgggacggta
     4261 ataccgatga cggggttgaa gcggccgatg gcacttattt ctataacctg actgcccgtg
     4321 gagtggacgg cctggaatat tcgcgccagg gaaccgtaat gctgcttcgc catggtgctg
     4381 tggcttttcc gaatcccgtg ctgaattcgg tcagcattaa gacattcggc cccctggatc
     4441 ccccggttta tatcgaaatt ttctccgttt tcggagaaaa agtgtattca gggttggaaa
     4501 cccaacccgg gaatatccat cttgacctgg ccgagctaag taaaggcatt tatttcatcc
     4561 gggtgcatga caaccggaaa gagtattatg tcaggatcat ta
//
LOCUS       DAOQXS010000212         4598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19090_length_4598_cov_3.609509, whole genome shotgun sequence.
ACCESSION   DAOQXS010000212 DAOQXS010000000
VERSION     DAOQXS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_4598_cov_3.609509"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PKM34_06860"
     CDS             <1..1256
                     /locus_tag="PKM34_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNQ83346.1"
                     /translation="TPGDPNFRLAKNEQQKWAITLAAATQGTQIQFKFTRGSWETVEK
                     GQSGEEIGNRLFTYGNGSTVNIVIYNWAQGGGGGSTAAENVIIMDDAFEMPQLGRTRR
                     IWLYLPPGYETSENNYPVLYMHDGQNLFDDATSFAGEWHIDETLNDLFDQGINVPIVV
                     GIDNGGAERLNEYTPWKHVVHGGGDGELYMQFITETLKPYIDGHYRTLPGREHTGIMG
                     SSLGGLISHFGALDNQDIFSKAGIFSPSYWFSDSLWAFTREAGKQDAMRLYLMCGGME
                     GQNTINDVMDMQDSLLVSGYQEEDLSVKIIPGGQHNENLWSGDFGEAYLWLFAAYAND
                     ITEHVRYEVIRLYPNPATGMVSFPADFPRDIDSVDIIDMQGVLVNRIEPVTGREIDIS
                     GLSNGIYVILVRTGNTWYQGKVVKE"
     gene            complement(1289..1921)
                     /locus_tag="PKM34_06865"
     CDS             complement(1289..1921)
                     /locus_tag="PKM34_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNQ83347.1"
                     /translation="MEIGNWKFGNQEPGTRNLELFHPTNDQRKILILPVSPLFSQISL
                     MTMVNRRALVPLYVLGIMFYVLIINDVKVVAQDGAIRNEILMEVNKLRKEGCTCGEEK
                     MPPVMEVTWNDKLEKAAAKHADDLFKNSRFSHMGNDGSEASDRVEKAGYKWSAVGENI
                     GKGYATAAQVVNGWRQSPGHCRNMMDPSFREIGAARKGEIWVLNLGAWRE"
     gene            2245..2646
                     /locus_tag="PKM34_06870"
     CDS             2245..2646
                     /locus_tag="PKM34_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ83348.1"
                     /translation="MKLKVIIERGNDGSFGAYIGSDNAPFGIIGDGKTALEAKTDFLN
                     SYEEMKAYYMETNKEFTECEFEFQFDVASFLAYYGCILSLAGMERLTGVNQGQLSHYV
                     TGRRKPSKKTIEKIEKKLHEFGRELNQVEFI"
     gene            3522..3782
                     /locus_tag="PKM34_06875"
     CDS             3522..3782
                     /locus_tag="PKM34_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83349.1"
                     /translation="MFFGQVNTVLVYKSIIKGRYTFCRNVSSPSGFPVKNFPDFILKV
                     VHLAQESVVHFRQELLVRYAQEWVVQFGQEYSGDLQKHNAAL"
     gene            complement(3873..>4598)
                     /locus_tag="PKM34_06880"
     CDS             complement(3873..>4598)
                     /locus_tag="PKM34_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83350.1"
                     /translation="TKTEEGLTCGNSYSRYAWAYNDCGYSTPVTLIASTLGYTLACGD
                     PFTINHVAGNVAPVNKTVTYTTVTNILGEPTKCWITSNLGSDRQATAVNDATEESAGW
                     YWQFNRMQGYKHDGTTRIPNITWINSIDENSDWLAANDPCALLLGSGWRLPTSTEWIN
                     VDASGNWTNWNGPWNSALKMHAAGGLLSSGGSLFDRGSKGYYWSSSKYSNAYGWELFF
                     YSSNSTMNSHHKVYGFSSRCLRD"
BASE COUNT         1168 a         1066 c         1107 g         1257 t
ORIGIN      
        1 ggacccccgg tgatccgaac ttccggctgg cgaagaacga acagcagaaa tgggcgatca
       61 cgctggcggc ggcaacacag gggacccaga ttcaattcaa gttcacccgc gggagctggg
      121 agacggttga aaaagggcaa agcggggagg agatcggaaa caggctcttc acctacggca
      181 atgggtcaac ggtgaatatc gtcatttaca actgggcgca gggaggtggt ggaggcagta
      241 cggctgccga aaatgtgatc atcatggatg atgcctttga gatgccgcaa ctgggccgca
      301 cccgccggat ctggctttac ctgcctcccg gttatgagac ttcggaaaac aactacccgg
      361 tgttgtatat gcacgatggc caaaaccttt tcgacgatgc cacttccttt gccggggaat
      421 ggcatatcga cgaaacatta aatgatcttt ttgatcaggg gattaatgtc ccgatcgtgg
      481 taggtatcga caatggcggc gcagagcggc tgaatgagta cactccctgg aagcatgttg
      541 tgcacggagg aggcgatggc gaactctaca tgcagttcat cacggaaacg ctgaaacctt
      601 atatcgacgg gcactaccgc actttgccgg gccgagagca taccggcatc atgggcagtt
      661 ctctcggcgg cctgatctcc cattttggag cgctggataa ccaggatatc ttcagcaaag
      721 ccggcatctt ctctccctct tactggttct ccgacagcct gtgggcattt acccgtgaag
      781 cgggaaagca ggacgccatg cgcctttacc tgatgtgcgg cggcatggag gggcagaata
      841 caatcaatga tgtgatggat atgcaggaca gcctgctggt aagcggatac caggaagagg
      901 atctgagcgt gaagatcatc cccggcgggc agcacaacga aaacctctgg agcggcgact
      961 tcggcgaagc ctacctctgg ctgtttgcgg cctatgccaa cgatataacc gaacatgtcc
     1021 gttatgaggt aatccgccta tatcccaatc ctgccacagg catggtatct tttcctgcag
     1081 attttccccg ggatatcgac agcgtggata taattgatat gcaaggtgtc ctggttaacc
     1141 gaatcgagcc ggtgacagga agagaaattg atatctcggg attatcgaat ggaatctatg
     1201 tgatcctggt gagaactggc aatacctggt accaggggaa agtggtgaag gagtgaaaat
     1261 tgtgctttac ttgatgttcc gttttgtgtt attccctcca cgcgccaagg tttaaaaccc
     1321 agatctctcc tttccgggcc gctccgatct ccctgaagga tggatccatc atattccggc
     1381 agtgccccgg gctctgtcgc catccattca ccacctgggc cgcggttgca tatcctttcc
     1441 ctatattttc tcccacagcc gaccatttat agcctgcttt ctcaacgcgg tccgatgctt
     1501 cggagccatc attgcccata tggctgaacc gcgaattttt aaaaagatca tcggcatgtt
     1561 ttgcagcagc tttttcaagc ttgtcgttcc aggtaacctc catgaccggc ggcatctttt
     1621 cctctccgca agtgcatcct tctttgcgca gcttattgac ctccattaga atttcgttcc
     1681 gtatcgcccc atcctgtgct actaccttta catcattaat aatcaacaca taaaacataa
     1741 tacccagcac atacaaaggc accagagcac gacggttcac catagtcata agcgatattt
     1801 gagagaataa cggtgaaacc gggaggatta gtatttttct ttggtcgttg gtcggatgaa
     1861 agagttccag gttcctggtt cctggttcct ggttgccaaa tttccaattt ccaatttcca
     1921 ttttttctcg aagaggttcg gcgattttca gaggcccaaa gtctggtatt gaccggaaag
     1981 tgaaggaata gctccgggct ccggcccgaa aagcgcataa aacaaaacta atttgtttgt
     2041 taaatcttta ccccgtaggg gttttccgtc ttgtagaaat cgtggccggt tatggtaaat
     2101 tccccctccc tttgatgccg tttcctaaag ttgcagtgtt tcacagggag gggcagggga
     2161 gggtgaaaaa ataatggaat tttaataatt gatattaata ttaatatctt tataatctct
     2221 ggtagaagtt tagtatttga ggatatgaag ttgaaggtaa ttattgaacg cggtaatgac
     2281 ggcagtttcg gagcctatat tggttctgac aatgcgcctt ttggaatcat aggagatgga
     2341 aaaactgcgc tggaagcaaa gacggatttt ttaaattcgt atgaagagat gaaagcatat
     2401 tacatggaga caaataaaga gtttactgaa tgtgaatttg aatttcaatt tgatgtggcc
     2461 tcatttttag cttattatgg atgtatctta tccttagccg gcatggaacg gttaactggt
     2521 gtaaatcagg gacagttaag ccattacgta acaggccgcc gaaaaccgtc aaaaaaaacc
     2581 attgaaaaga ttgaaaagaa attgcacgag tttggccggg aattaaacca ggtggagttt
     2641 atatgaaata ccgattcagt cgtcggtcgt cagtcaacta ttaactcaaa acccctccaa
     2701 gtcccatagg gacgccatta tggtagaatg ttggtgacag gatttcccat ttcccctccc
     2761 tttgatgccg tttcccaaag ttgcagtgtt tcacagggag ggtgagggga gggtgattca
     2821 tagaattgat tcttaagtga ttatttcgga caaacaaggc cacctgtttc ggtgtaaatt
     2881 gagccactca tttcggacga aagtgagtct ctatttcaaa gggcaacatg agggtaatag
     2941 tattaattta tttccattga ttttcttctt aacgattctc ctttaatttc aaatcggtgt
     3001 gcagaacctg acaatctgtc cataatcgca tcggccaggg taggttcatt gattttttag
     3061 tgccatttgt ttatcggtca ctggtagctt ataatggtgg accgtttgcc gtaacgatct
     3121 tccagcactt gcaaaaaagc tattctgacc tggttgtcca gagtagctgc atccatcccg
     3181 atgatcacac aggtattcaa gccataaaag ctggcgtgtt actcccggac gcttgagttc
     3241 ttcaggaaat gctctatcgg atcctccagt aactcgctac acccggaagt taatgcacag
     3301 taccaaggag gataaaccag ttccgccagc tcggcatctt tcatctttaa aagctcaacc
     3361 ggctcctagc cgctttgttt gattcttgca tgataatccc tgagcgtatc ccgttgtatg
     3421 ccggccagtt agtccacttt ttgttcagag cagccattag ccagtagttg aatgatctta
     3481 cgaatttgta acatcgtttt tcgtttgttc gccatgtcaa cttgtttttc ggccaagtta
     3541 acacggtcct cgtttacaaa tccatcataa aagggcggta cactttctgc aggaatgtca
     3601 gttctccatc cggcttcccg gtgaaaaact tcccggattt tatcctaaaa gtggtacacc
     3661 ttgcccagga atcggtggta cactttcgac aggaattact ggtacgttat gctcaggaat
     3721 gggtggtaca gtttgggcag gaatattcag gtgatttgca aaagcataat gcagccctct
     3781 gatcttctca aacctttgcc atggaaaacc ggctaatccg taatctttta attcgacaat
     3841 aaatttgtca aacaagctaa tgagttaaat ggttagtctc tcaagcaccg actgctaaaa
     3901 ccgtacacct tgtggtgact gttcatagta ctattgctgc tgtaaaagaa caattcccat
     3961 ccgtaagcgt tactgtactt tgaactgctc cagtagtatc cttttgagcc tctgtcgaac
     4021 agtgaaccac cgctgctgag cagccccccg gctgcatgca tttttaaggc tgagttccac
     4081 ggaccgttcc agtttgtcca gtttccactt gcatcaacat tgatccattc ggtagaagtt
     4141 ggtaagcgcc atccgctgcc taacaaaagg gcgcacgggt cgttggcggc aagccaatct
     4201 gagttttcat cgatactgtt tatccaggtt atatttggga ttctggtagt accgtcatgt
     4261 ttatatccct gcatccggtt aaactgccag taccaaccgg cagattcttc agtagcatcg
     4321 ttaacggcgg tggcctggcg gtcggagccc aggttactgg taatccagca ttttgttggc
     4381 tcccctagga tattggtaac cgtggtatag gttaccgttt tattaaccgg cgcgacattt
     4441 cctgccacgt gattaatggt aaacggatct ccacaagcta aggtgtaacc cagggtagat
     4501 gctattaaag ttaccggggt ggaatatcca cagtcattat aggcccaggc ataacgggaa
     4561 taagaattac cgcaggtaag cccctcttct gtttttgt
//
LOCUS       DAOQXS010000213         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19094_length_4597_cov_3.771466, whole genome shotgun sequence.
ACCESSION   DAOQXS010000213 DAOQXS010000000
VERSION     DAOQXS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19094_length_4597_cov_3.771466"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKM34_06885"
     CDS             complement(<1..832)
                     /locus_tag="PKM34_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNQ83351.1"
                     /translation="MAKNGNKELEALISLLDEPDEDIFLTIRDRIFAYGLDAVPFLES
                     VWENAFDPLVQHRIEEIIHNLQFEDLKREMMQWARFGYHDLLQGALLVSKFQYPDLKE
                     DEIIKKVGQFSQDIWLELNQNLTGLEKVKVINHILFDTHKLAGNLANVNSADNYFLNS
                     LLDTKKGSPLLLGIVYISIAQSLKIPVYGVDLPRHFVLAYTDEIILAPRDIPEGDVMF
                     YINPFNKGAVFTKNEIDLFIKQLKLERKESYFTPCDNKTIIRRMMNELIYIYDQSGNP
                     V"
     gene            complement(884..1732)
                     /locus_tag="PKM34_06890"
     CDS             complement(884..1732)
                     /locus_tag="PKM34_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNQ83352.1"
                     /translation="MIVNSDGSIYHLKLRPEEIADLVLVSGDPARIGMISDHFDTVIF
                     RRQNREFVSHLGTYNGARILALSTGIGPDNIDIVMNELDALANIDLVSREILPEKRSL
                     TIIRIGTSGTIHADIPVGSFVVSSHGLGMDGSLNYYAGLKEVTDSELTQAFARQTAWP
                     AHLPVPYIIPGSSDLVEKLSGAGITGITATAGGFYGPQGRQIRLNSSFPEMLPALARF
                     QHNGHRIINFEMETSSLYGLGRMLGHRAASICVLLANRATGHYTRNIRQDEERLIGYV
                     LDKMTS"
     gene            1874..3121
                     /locus_tag="PKM34_06895"
     CDS             1874..3121
                     /locus_tag="PKM34_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNQ83353.1"
                     /translation="MEAEIISIGDELLIGQVINTNASWMARRLAENGIRARNMVVVPD
                     EAEDIRKAVDVAFGRSEVILVTGGLGPTKDDITKKVLCDYFGTRLVFHQPSFDNILRL
                     FPTRASNLSPANRQQADIPENCTPVMNQFGTAPGMIFRKEGRILVSMPGVPFEMEAMM
                     NNDVIPMLKKSANSDVMVCKTLLTQGVGESYLSELIAVWEESLPENMKLAYLPQPGIV
                     RLRLNAYGDNRETLQEQIDFQSAKLEEIIPDLIFGYGDDTMEAVAGKLLREKGKSLST
                     AESCTGGYLAHLITSVPGSSDYFKGSVVAYANDIKMALLGVSEGSLARHGAVSEEVVR
                     EMALGALEKFNTDYAVSTSGVAGPDGGTPEKPVGTVWVAVASRIGATTKLFRLGDHRG
                     RNIRRAALSALNMLRLELLKTKS"
     gene            3134..3685
                     /locus_tag="PKM34_06900"
     CDS             3134..3685
                     /locus_tag="PKM34_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNQ83354.1"
                     /translation="MKSMNVKSLLALFITFIIFTACSSSMKSLQTVPKVDLERYSGKW
                     YEIARLPNSFEKGLICCTAEYSLRDDGRITVINTGYSEKDPSKISSVKGKAWNPDKNE
                     PGRLKVQFFWPFSGGYYIFHLDPEYRYVLVGSPSRKFFWLLARNPVIEENLYGELVRI
                     AADNGFATQNLIRVSQECHKQAK"
     gene            3736..>4597
                     /locus_tag="PKM34_06905"
     CDS             3736..>4597
                     /locus_tag="PKM34_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ83355.1"
                     /translation="MPFSNIAYRTPKYYFSDTSFNKLMRRRIYHVLLISSTYDAFVLE
                     DDGRIDEQIFIEYVSLNLRYPPQFILADSEKKAFDILHDEKIDLVVVMLSSEEMDTFS
                     LAEKIKMKNPAIPIVVLTPFSREVTLKLNDTTKSAVDYIFSWLGNADLLLAIIKLIED
                     KMNVDHDIREVGVQAILLVEDSIRFYSSYLPNIYKIIFKQSKDFMTEGLNEHQMMLRM
                     RGRPKIMLATTYEEALGLYEKYKRNLLGIITDMSYPRNGKQEKYAGVKFCEKVQRDDP
                     FMPILLQSSDK"
BASE COUNT         1118 a         1172 c         1238 g         1069 t
ORIGIN      
        1 tcaccggatt gcccgactgg tcgtagatat agatcaattc gttcatcatc cgccggatga
       61 tggttttgtt gtcgcagggg gtgaaatagg attcttttct ctccagttta agctgtttga
      121 tgaacaggtc gatctcattt ttcgtaaaaa ccgctccctt gttgaaagga ttgatgtaga
      181 acatcacgtc gccttcggga atgtcgcgcg gggccagtat gatctcatcg gtataagcca
      241 gcacgaaatg ccgcggtaag tcgacaccgt aaaccgggat cttcaggctc tgggcaatgg
      301 agatgtaaac gatccccagc agcaatgggc tgcctttttt cgtgtccagc agcgagttga
      361 ggaagtaatt atcggccgag ttgacattgg ccaggttgcc cgccagtttg tgcgtgtcga
      421 aaagaatgtg gttgatcacc ttgacttttt cgagtccggt gaggttttgg ttcagttcga
      481 gccagatatc ctgcgagaac tggccgacct tcttgatgat ctcatcttcc ttcagatcag
      541 gatactggaa tttgctgacc agcagtgcgc cctgcaagag atcgtgatag ccgaaccggg
      601 cccattgcat catctctctc ttcaggtctt caaattgcag gttatgaatg atttcctcaa
      661 tccggtgctg gaccaacggg tcaaacgcat tctcccagac gctctccagg aaaggcaccg
      721 catcgagccc gtatgcgaaa atgcggtcgc ggatcgtcag gaagatatcc tcatccggtt
      781 catcgagaag actgatcagc gcctccagtt ccttatttcc gttttttgcc atgtaatacc
      841 gcaattgtca atatccctgt aacaaagata atgccttgtt ggttcagctt gtcattttgt
      901 cgaggacata tccgatcagg cgctcctcat cctggcgtat attccgcgtg tagtgccctg
      961 tagccctgtt ggccagcaat acgcagatgc tggctgcccg gtggccgagc atccggccca
     1021 ggccgtacag cgacgaggtt tccatctcga agttgatgat ccggtgaccg ttatgctgaa
     1081 accgggccaa tgccggcagc atctccggaa acgagctgtt cagcctgatc tgacgcccct
     1141 gggggccgta aaaaccgccc gcggttgccg taatgcctgt gatcccggcg cccgaaagct
     1201 tttccaccag gtcgctgctg cccgggataa tgtagggcac cggtaaatgc gccggccatg
     1261 cggtctgcct ggcaaacgct tgggtcaatt cgctgtcggt gacttctttt aaacctgcgt
     1321 aatagttcag cgaaccgtcc atgcccagcc cgtgggaaga gaccacaaag gaaccgaccg
     1381 gaatatcggc gtggatcgtg cccgaggtgc cgatccggat gatcgtcagg gaacgttttt
     1441 caggcaatat ttccctggaa accaggtcga tattggccag cgcatcgagt tcgttcatga
     1501 caatgtcgat attatccggg ccaatgccgg tcgatagggc caggatccgg gcgccgttgt
     1561 acgttccaag gtggctcaca aattcccggt tctgccgcct gaagatcacc gtgtcgaaat
     1621 ggtctgaaat catgccgatg cgggccggat cgcccgatac cagcaccagg tccgcgatct
     1681 cttccggcct cagtttcagg tgatatatgc ttccgtctga attaacgatc aaatccgatt
     1741 ctttcataac tctgtctctc ctctgtgtaa ctctgtgtac tccgtggtga aaaaaatcac
     1801 cttatgtaaa ggacatcaaa attaaaattc cccccgttac attaccattc ccgggagata
     1861 ttaattttac accatggaag cggaaattat cagtatagga gatgaactcc tcatcgggca
     1921 ggtaatcaat accaacgcgt cgtggatggc acggcgactg gcggagaacg gaatccgggc
     1981 caggaatatg gttgtggttc cggatgaggc ggaagatatc cggaaagcgg tggatgtggc
     2041 cttcggccgg agcgaagtga tcctggtgac gggaggcctc ggcccgacaa aggatgatat
     2101 caccaagaaa gttttgtgcg attattttgg aacccggctg gtgtttcatc agccctcctt
     2161 cgataacatc ctgaggctct tccccacccg ggcgagcaac ctgagccccg ccaaccggca
     2221 gcaggccgat atccctgaga actgcacccc ggtgatgaac caattcggta cggctccggg
     2281 aatgatcttc aggaaagaag ggcggatcct ggtgtcgatg cccggagtcc ctttcgaaat
     2341 ggaggccatg atgaacaacg atgtcatccc gatgttgaaa aaaagcgcca atagcgatgt
     2401 aatggtgtgc aagaccctgc tgacgcaggg cgttggcgaa tcctacctgt cggagctgat
     2461 cgctgtctgg gaagagagcc tgccggagaa catgaagctg gcctacctgc cgcagcccgg
     2521 tattgtcagg ctcaggctga atgcctacgg ggataacagg gaaacattgc aggagcaaat
     2581 tgactttcaa tccgcaaagc ttgaggagat catccccgat ctgattttcg gatatggcga
     2641 tgatacgatg gaagctgtag cagggaaatt gctcagggag aaaggaaaat cgctgagcac
     2701 ggctgaaagc tgtaccgggg gctatctcgc gcatctgatc acgagcgtgc cgggctcatc
     2761 ggattacttc aagggctcgg tcgttgccta tgccaatgat atcaagatgg ccttgctcgg
     2821 cgtttcggaa ggatcgctgg caaggcacgg ggccgtcagc gaagaggtgg tcagggagat
     2881 ggcgctgggg gcgcttgaaa agttcaatac cgattacgcg gtttccacat ccggagtggc
     2941 gggtcctgac gggggcacgc cggaaaaacc ggtcggtact gtttgggttg cagtggcctc
     3001 ccgtattgga gccactacaa agctcttccg gctcggcgac cacaggggcc gcaatatccg
     3061 ccgcgccgcg ttgtcggccc tgaatatgct caggctggaa ttgttgaaaa cgaaatctta
     3121 aaaccaaaat gccatgaaat ccatgaatgt gaaatccctt ttggcgttgt tcatcacttt
     3181 cattatcttt actgcttgct catcttctat gaaatcactg caaactgtcc caaaggttga
     3241 cctggagaga tattcgggaa aatggtacga aatagcccgc ttgccgaatt ccttcgagaa
     3301 agggctgata tgctgcacgg cggaatacag cctgcgggac gacggccgga tcacggtgat
     3361 caataccggt tatagtgaaa aagacccgtc gaaaataagc tcggtgaagg gcaaagcgtg
     3421 gaaccctgat aaaaatgagc cgggcaggct gaaggtgcag ttcttctggc cattcagcgg
     3481 cgggtactac atctttcacc tcgatccgga ataccggtat gtccttgtcg ggagtccgtc
     3541 ccggaaattc ttctggctgc tggcccgtaa tccggtcatt gaggagaacc tttatggcga
     3601 actggtccgg atcgctgcag ataacggatt tgcaacacag aacctgatca gggtgagcca
     3661 ggaatgccac aaacaggcaa aatagttcaa attccaaagt ttagcattaa gaattaagaa
     3721 ttcaacatta ttccgatgcc tttttccaac atagcctacc ggacgccaaa atattatttc
     3781 agcgatacct cattcaacaa gctgatgcgg cggaggatct atcatgtcct gctgatatca
     3841 agcacttacg atgcctttgt gctggaagac gacggccgga tcgacgagca gattttcatc
     3901 gaatatgtct cactgaacct tcgctatccg ccgcagttca tcctggccga ttcggaaaag
     3961 aaagcgttcg acatcctgca cgacgagaag atcgacctgg tggtggtcat gctcagctcc
     4021 gaagagatgg atacattcag ccttgccgag aagatcaaga tgaaaaaccc ggcgatcccg
     4081 atcgttgtcc ttacaccctt ctcacgcgaa gtgaccctca aactgaacga tacgacgaaa
     4141 agcgctgtcg attatatctt cagctggctc ggaaatgcag atcttctgct ggccatcatt
     4201 aagctgatcg aagataaaat gaacgtcgat cacgatatcc gcgaggtagg ggtccaggct
     4261 attttgctcg tggaagactc catccggttt tattccagtt atcttcccaa catctacaag
     4321 atcatcttca aacaatcgaa agacttcatg acggaagggc tgaacgaaca ccagatgatg
     4381 ctgcggatgc ggggacgccc caagatcatg ctggcgacca cgtatgagga agcgctgggt
     4441 ctctatgaga aatacaagcg caacctgctg gggatcatta ccgatatgag ctatccgcgc
     4501 aacggcaaac aggagaagta cgccggtgtg aaattctgcg aaaaagtgca gcgcgacgat
     4561 ccgtttatgc ccatcctgct gcagtcgtcg gataaag
//
LOCUS       DAOQXS010000214         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19118_length_4593_cov_3.624284, whole genome shotgun sequence.
ACCESSION   DAOQXS010000214 DAOQXS010000000
VERSION     DAOQXS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19118_length_4593_cov_3.624284"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>233
                     /locus_tag="PKM34_06910"
     CDS             <1..>233
                     /locus_tag="PKM34_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83356.1"
                     /translation="SLSNKTATSWEIPEDITVIDHYNLNDYNEFCMSNDKKVIIMAVE
                     REDTYGEKDLYVSFNIEGNLWSVPLNLGPVLNS"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <244..1152
                     /locus_tag="PKM34_06915"
     CDS             <244..1152
                     /locus_tag="PKM34_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ83357.1"
                     /translation="EISPFLAADNTTLYFSSDGHPGHGDNDIFLARRLDETWMNWSKP
                     ENLGKPINSSDWDAYYSVPASGEYAYFVSSKSGFGNSDIFRIKLPEMARPNPVVLVKG
                     KVLNQKTKTPVQAVINYFDLQNSAISGTAMSNPETGYYQITLPAGRLYGFLASSEKFI
                     SVNENVDLKDLDEYQELERDLYLVPIEIGEKVRLNNIFFETGKWDLLPESFAELDKLV
                     RILTDNPNMEIEINGHTDNVGNDESNLMLSQKRAAAVVNYILDKGISNTRLDSAGFGE
                     AQPVATNDTDEGRRLNRRVEFMIVKM"
     gene            complement(1252..4050)
                     /locus_tag="PKM34_06920"
     CDS             complement(1252..4050)
                     /locus_tag="PKM34_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNQ83358.1"
                     /translation="MKNNKYIPRQIVLIFIILLPYWSVLSTFSQSNEDCLMCHEDPTL
                     TAIRQGKTVSMFVNEKILISSVHDGMECTSCHPDAGVEEFPHPEVLPPVDCGFCHDKA
                     FAEFDRGIHGQALRLNALYAPTCKECHGSHDILSRTNPKSRTYKMNIPVLCGKCHREG
                     APVARTYNISEHNILENYSESIHGEGLFKRGLIVTATCNDCHGNHLVLPHTSPNSTIS
                     INNIANTCMQCHAEIERVHTKIIKEELWEKEPGAIPACTDCHPPHKVNPQNIVATISD
                     KSCLECHNSDAVHKIADGQRVSLKVVPEDITFSVHKNINCVKCHSDVSPTLARPCETA
                     NRVDCSNCHAEVSNVYAASGHGQAYGRREANAPYCTDCHGTHRVKSRYDDTSPTYRTA
                     IPALCGDCHRSDGKASQVEGLKEINAYADYSTSVHGRGLTEKGLLPSAVCTDCHTSHF
                     ILKDSDERSSVFPKNIPATCATCHKGIYDEYIKSDHAITVHDEKLQYPTCADCHSAHV
                     ITDISQDVFVNEVTNQCGKCHKDLADTYFETYHGKAYLLGSEKAAKCSDCHGSHSIFM
                     ASNPMSSVGSRNIVETCKQCHPDANQRFTGYLTHATHHNKAKYPALYYTFWGMTTLLV
                     VVFAFFGIHLLLWLPRSFQAMKKKRMKQAETGEELHIRRFSRSQRITHLFVILSFILL
                     ALTGMTLKFAALPWAAFLADLMGGVHVAGNIHRFAAVITFGYFIYHVYYLIKSKIRNK
                     TKWGSFIFGKNSLMFNKQDLKDFWATFKWFLGLGPKPGYGRWTYWEKFDYFAVFWGVA
                     VIGFSGLILWFPEFFTTIFPGWLINVAMIIHSDEALLAVGFIFTIHFFNTHLRPDAFP
                     MDKVIFTGTVPMEDYKHERPREFEELEQSGRLEEVLVKPEQNKEIDRIIRVFGFTMLA
                     IGLTLVVLIIYSMLFGYN"
     gene            4319..>4593
                     /locus_tag="PKM34_06925"
     CDS             4319..>4593
                     /locus_tag="PKM34_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ83359.1"
                     /translation="MNNKKILVPVDFTRLSDKVIEHAATIGIRAGSDLVLLHCADRSA
                     DLDTLKIKLAEKAVKASRKFNITCDSKVRTGNIFTAIPEEADDSSYYL"
BASE COUNT         1170 a         1112 c         1171 g         1130 t
ORIGIN      
        1 tcagcctctc caacaaaacc gccacttcgt gggagatccc ggaagatatt acggttatcg
       61 accattataa cctgaatgat tacaatgagt tctgcatgtc gaacgataaa aaggtgatca
      121 tcatggcggt tgagagggaa gatacttatg gcgaaaaaga cctgtacgtt tcattcaata
      181 tcgaaggcaa cctctggtcg gtgccgctta acctggggcc ggtgctcaat tccnnnnnnn
      241 nnngaaatat cgcctttcct ggccgcggat aatacgacgc tctacttcag ctccgacggt
      301 catcccgggc acggcgacaa cgacatcttc ctggcccgcc gtcttgatga gacctggatg
      361 aactggagca aaccggaaaa tctcggcaag ccgatcaatt cgagcgattg ggacgcctac
      421 tacagcgtgc cggcctccgg cgaatatgcc tattttgtat ccagcaaatc gggctttggc
      481 aacagcgata ttttccggat caagcttccc gagatggccc gtcccaaccc ggtggtgctg
      541 gtcaaaggaa aggtgcttaa ccagaagacg aaaacaccgg tgcaggccgt catcaactat
      601 ttcgatctcc agaattcggc catatccggc accgccatga gcaatcccga aacaggctat
      661 taccagatca cgctgccggc agggaggctt tacggcttcc tggcttcgag cgaaaaattc
      721 atctcggtta acgaaaatgt tgacctgaaa gacctggatg aataccagga actggagcgc
      781 gacctctacc tggtcccgat cgagatcggc gaaaaagtaa ggctgaacaa catctttttt
      841 gagaccggca aatgggatct cctccccgaa tcattcgccg aactcgacaa gctggtcagg
      901 atactgaccg acaatccaaa catggagatc gagatcaacg ggcataccga taatgtcgga
      961 aacgatgaat ccaacctgat gctgtcgcag aaaagggcag ccgccgtggt gaattacatc
     1021 cttgacaagg gcatatcaaa cacgcgcctt gattcagccg gttttggcga agcgcaaccg
     1081 gttgccacga acgataccga tgaaggccgc cgactgaacc gcagggtgga gtttatgatc
     1141 gtgaagatgt aggttggtca tcagctcgct gcgctcgcgt cattggaaat tatgggaacc
     1201 ttggaacctg gaacctggaa ccttggaacc tggaacctgg aactctggaa cctaattgta
     1261 cccgaacagc atcgaataga tgatgagcac caccagtgtg aggccgatgg ccagcatggt
     1321 aaagccaaag acccgtatga tcctgtcgat ctctttgttt tgttcgggct tcaccagtac
     1381 ctcctccagc cttcctgatt gttccagttc ttcaaactcc ctcggccttt catgcttata
     1441 atcctccatg ggaacggttc cggtaaagat gaccttatcc atcgggaatg cgtccggacg
     1501 aagatgggtg ttgaagaaat ggatcgtgaa gatgaatccg acagccagta aggcctcatc
     1561 gctgtgaatg atcatggcaa cattgatcag ccatcccggg aagatagtcg tgaaaaattc
     1621 ggggaaccag aggatcaggc ctgaaaatcc gatgacggcc actccccaga atacggcaaa
     1681 gtagtcgaac ttctcccagt aggtccatct gccgtaaccc ggcttgggcc ctaatcccag
     1741 gaaccatttg aatgtggccc agaaatcttt caggtcctgt ttattgaaca taagggagtt
     1801 cttcccgaag ataaagctcc cccatttcgt tttgtttctg attttggatt tgatcagata
     1861 ataaacatga tagataaaat agccgaaagt aatgacggcg gcaaagcgat ggatatttcc
     1921 ggcgacatgg actccgccca tcagatcggc caggaaagcg gcccagggca aggcggcgaa
     1981 tttcagtgtc attccggtca atgcaagcaa gataaagctg agaataacga acaggtgggt
     2041 gatccgctgg ctccgggaga accgtcggat atgcagctct tcgcccgttt ccgcctgctt
     2101 cattcttttc ttcttcatcg cctggaacga tctgggcagc cagagtaaca ggtggatccc
     2161 gaagaaggca aataccacga caagcagggt ggtcattccc cagaatgtat aatacaatgc
     2221 gggatatttt gctttattgt gatgggtagc atgggtcagg tagcccgtaa accgctggtt
     2281 ggcatccgga tggcactgtt tgcaggtctc gacgatatta cggctgccga cggaagacat
     2341 cggattgctg gccataaata tactgtggga gccatgacag tcggaacatt tggccgcttt
     2401 ttcagagccc agcaggtatg ctttgccgtg atatgtctcg aaataagtat ctgcaaggtc
     2461 cttatggcat tttccgcatt ggttggtcac ttcgttcaca aagacatcct gtgaaatatc
     2521 ggtgatcaca tgggccgaat gacaatcggc gcaggtcgga tactgcagtt tttcatcatg
     2581 aacagtaata gcatggtcgc tcttgatata ttcatcataa atgcctttgt gacaggtagc
     2641 gcacgtagcc ggtatatttt tcggaaatac cgacgatcgt tcatccgaat cctttaagat
     2701 aaaatgggag gtatggcaat cggtgcacac ggcacttggc aacaggcctt tttcggtcag
     2761 accccttcca tggacgctgg tggagtaatc ggcataggcg ttgatctctt tgagcccttc
     2821 gacctgcgat gctttcccgt cggaccggtg gcagtcgccg caaagggcag ggatagcggt
     2881 acgataggtg ggcgaagtat cgtcatatct cgatttcacg cggtgggtcc cgtgacagtc
     2941 ggtgcagtag ggtgcattgg cttctctccg gccgtaggcc tgcccgtggc cactggcggc
     3001 atacacgttc gacacctccg catggcagtt ggagcaatca acccggttgg ccgtttcaca
     3061 ggggcgggcc agtgtcggag aaacgtccga atgacatttc acgcaattga tattcttgtg
     3121 tactgagaaa gttatatctt cgggcactac ctttaacgat accctctggc cgtcggcaat
     3181 tttatgcaca gcgtcggagt tgtggcattc caggcaggat ttatcggaga tagtggcgac
     3241 gatattctgg ggattcacct tgtgcggcgg gtggcagtcg gtacatgccg gaatagcgcc
     3301 cggttccttt tcccatagct cttccttgat aattttagtg tgtacccttt cgatctccgc
     3361 gtggcattgc atacaggtat ttgcaatatt gtttatagag atggttgaat tgggactggt
     3421 atgcggcagc acgagatgat ttccgtggca atcgttgcag gtggccgtaa cgatcaggcc
     3481 tcttttgaaa aggccttcac catgaatact ctccgagtaa ttctcgagaa tgttatgttc
     3541 gctgatgtta taggtccggg caaccggcgc cccttcgcgg tggcatttgc cgcacagcac
     3601 cgggatattc atcttatagg tgcgggattt cgggttggtg cgggaaagaa tatcgtggct
     3661 tccatggcac tccttgcagg taggtgcata aagtgcattc agccgcaatg cctgcccgtg
     3721 aatcccacgg tcgaactcgg caaaggcctt gtcgtggcag aaaccgcaat cgaccggagg
     3781 aagtacttcg ggatggggaa actcctccac gccggcatcg ggatgacagg aagtacattc
     3841 catcccgtcg tggacgctgc tgatgaggat cttttcattg acgaacatcg acaccgtttt
     3901 cccctgccgg atagccgtaa gggtcgggtc ttcgtgacac atcaggcaat cttcattgct
     3961 ctgggagaaa gtggacaaga cagaccagta aggcagtaaa atgatgaata tcaacactat
     4021 ctgcctgggt atatatttgt tgtttttcac ggattgaaaa tttataaata cgaaaatatt
     4081 caaatatata cgaatgttat acgtcaggat gtaatttata aagattctaa gttggattac
     4141 ccttaatctc tcaatttctt tttataccag aacgaaaata gtccattcaa atattcattc
     4201 attatttaac aggtatttaa caaattggat atgtgtgttt tggcttgata agcaaaggaa
     4261 tgaactactt ttacagattc aaccattcac accttctgat tgaataataa tcttcacgat
     4321 gaataacaaa aagatactgg tccccgtcga ttttacgcgc ttatccgaca aagtcattga
     4381 acacgcagca accattggta taagggccgg ttccgacctt gtcctgcttc attgcgctga
     4441 ccgttccgcc gatcttgaca cgttgaagat caagctggct gaaaaggcgg taaaggcatc
     4501 caggaaattc aatatcacct gcgatagcaa agtcaggaca ggtaatattt tcaccgctat
     4561 accggaagag gccgacgaca gcagctatta cct
//
LOCUS       DAOQXS010000215         4590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19139_length_4590_cov_3.485777, whole genome shotgun sequence.
ACCESSION   DAOQXS010000215 DAOQXS010000000
VERSION     DAOQXS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19139_length_4590_cov_3.485777"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKM34_06930"
     CDS             complement(<1..1056)
                     /locus_tag="PKM34_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNQ83360.1"
                     /translation="MLNSLRLYAKYFIVSAISPAGKYLWLDTGRYIFNKDIFKKEPHK
                     EPDLQAKALMKAIAWLVRSQQANTDGGMGSFHLINKWSSSYPETTGYIIPTLLNFGRK
                     YDNDEVTGSAMKAAKFLLKIQKKSGGWQGGRVAENKPEIVFNTGQAIRGMMAAYNFSG
                     EQKYLDAASAAGRWLSGIQNPEGYWKEFALMNQPRVYDTFVDAPLLELYGLTGEESLK
                     NAAIRNLDWVVGEKMLENGWFEDCDNTIRRNDKPILHTIAYTLDGLVDCGLMLEDEKY
                     IAAARKGAAQLREIFPERGYLHGRYDRNWHGSEHMICTGSAQISIVWLKFFRISGERV
                     WYDAAGQMLDLLMTVQSR"
     gene            1195..2913
                     /locus_tag="PKM34_06935"
     CDS             1195..2913
                     /locus_tag="PKM34_06935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNQ83361.1"
                     /translation="MDNFVVSARKYRPATFDMVVGQQSITSTLKNAIRNNQLAQAFLF
                     CGPRGVGKTTCARILAKTINCMNPRDKIEPCDQCESCLSFNRSASFNIHELDAASNNS
                     VDDIRNLVDQVRIPPQVGKYKVYIIDEVHMLSAAAFNAFLKTLEEPPPYAKFILATTE
                     KHKIIPTILSRCQIFDFRRIVVKDIADYLAYVAKSEGVDAEEEALHVIAQKADGAMRD
                     ALSFFDQLVSFSGSQLTYQTVTEHLNILDHAYYFRIMDQALSQDTSGILLTLDEIISR
                     GFDGQHFLIGLGEHLRNLLVCQDPKTVTLMDISSNLQQKYAEQAGKCSPAFLLQALEI
                     HNQYDQSYKTANNKRLQLELVMLLISAAAVKSGSPEPEKKISLIPPPADPKKPGPEPG
                     IPLTPKPEENKTVAPPVAAVPKPSAALSASRTISIKPENHTPKKVGATGEETPAGPVN
                     QKEFTQEKLEKVWLYYTESIAGQYPNFYSILSTRKPVLKENFLIYLQLDNKTQEITLK
                     DRRADLLDFLRNELGNQSVQMESVVIETATQSKPYTPDEKFRSMVEKNPELKVLKEGL
                     DLEIEF"
     gene            complement(2965..>4590)
                     /locus_tag="PKM34_06940"
     CDS             complement(2965..>4590)
                     /locus_tag="PKM34_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNQ83362.1"
                     /translation="DQYVWQYFSHFLEQEPTPGVAYDFAFTNAMLPGITLEEVNAQAG
                     KWIIDKNRVVSLMAPDNPDIKVPSEQEVRDIITDVETEPITAYVDKVTDKPLMAAEPT
                     PAKVAKKGKDKTLGTTTWTFENGVKAVMKTTDFKDDEILMSAYSFGGSSLYETKDLMS
                     AMFASDISLESGLGEFDRIALDKKLTGVIASVRPVIKGSSEGLEGSCSVKDLETMLQM
                     VNLYFTAPRTDEVAFNAFIKRMKAMLDNKALEPESALQDTVTVLMANHHPRVRPLTSA
                     ILDETNLKKARSIFKTRFGDPGSFTFYFVGNIDPVEAKPLIEKYLGGLPKVSREETFK
                     DNNIRPPAGKVERVIEREMKVPKGTVYLTYTGTFDYDDFQSRLNLSALCDILDVRFVE
                     TIREEQSGTYGAAVYESMDKYPYENYSVTIFFDCDPQNAGKLKQIVYDEIGKLKTDGP
                     TEKDLHGVKENKIKVHQENLRQNNYWLGIIKNKDYYGTGLDEYMKYEDYVNSMSVGSL
                     KTAAQQFFGDNIIEAVLMPANMEDNISNPVHPE"
BASE COUNT         1179 a         1167 c         1033 g         1211 t
ORIGIN      
        1 cctgctctgt accgtcatca gcagatccag catttgaccg gccgcatcgt accagacccg
       61 ttcgcccgaa attctgaaga atttcagcca gacgatggat atctgggccg atccggtgca
      121 gatcatatgc tccgaaccat gccagttccg gtcgtatctg ccatgcaaat atccccgttc
      181 agggaaaatc tcccggagtt gtgcggcgcc tttccgtgca gcggctatat acttttcatc
      241 ttccagcatc aatccgcaat cgaccaggcc atcgagcgta taggcaatcg tgtgcagaat
      301 gggtttatca tttctccgga tggtattgtc acaatcttca aaccagccat tctccagcat
      361 tttctcaccg acgacccagt cgagattacg tatagccgca tttttcaggc tttcctcacc
      421 ggtaaggccg tacaattcga gcagcggggc atcgacgaaa gtgtcgtata ccctcggctg
      481 gttcatcagg gcaaattcct tccagtatcc ctccggattt tggattccgg ataaccagcg
      541 gccggctgcc gacgctgcat caaggtattt ttgttccccg ctgaaattgt atgctgccat
      601 catgccgcgg atcgcctggc ccgtattgaa aacaatctcg ggtttatttt cggccactct
      661 tcccccctgc catcccccgc tcttcttctg gattttcagt aaaaatttcg ctgctttcat
      721 cgcactaccg gtaacctcat cattatcata ttttcttccg aaattcaaca atgtaggaat
      781 aatgtacccg gtcgtttccg gataagagga tgaccactta tttataagat gaaaagatcc
      841 catcccgcca tcggtattgg cttgttgcga tctgaccagc caggcgatag ctttcataag
      901 cgctttagct tgaaggtcag gttctttatg aggttctttc ttaaaaatat ctttattaaa
      961 aatatagcgc ccggtatcca gccaaagata tttcccggcc ggtgaaatgg ccgacactat
     1021 gaaatatttt gcatataacc tgaggctgtt aagcatattt ttcagaaaat tctaccggca
     1081 aagataacga agtaaggctt gtttatattt tcccttaact aatatccgta atccgatcac
     1141 ggctggtcaa tctttattat ttttgaccaa tcgaaaagga ggaacacgca ggacatggat
     1201 aattttgtcg tttcagcaag aaagtacagg ccggccacat tcgatatggt tgtaggccag
     1261 caatccatta catcgacctt aaagaacgcg atccggaata atcagcttgc gcaggcattc
     1321 ctgttctgcg ggccaagagg agtaggcaaa acgacctgtg cacgtatcct ggccaagacg
     1381 atcaattgca tgaatcccag ggacaagata gagccctgcg accagtgtga atcgtgtctt
     1441 tctttcaacc gttcggcttc tttcaacatc cacgagctgg atgcggcatc caacaattcg
     1501 gtcgacgaca tccgaaacct ggtcgaccag gtcaggattc cgccgcaggt tggaaaatac
     1561 aaagtgtata tcatcgatga agtccacatg ctttcggcgg cggcattcaa cgcttttctg
     1621 aagacgttgg aggaacctcc gccatacgct aaattcatcc tggcgacgac ggaaaagcac
     1681 aagatcattc cgacgatcct ttcccgttgc cagatatttg atttcagacg gattgtagtt
     1741 aaagatattg ccgattacct ggcgtatgta gctaaaagcg aaggagtcga cgcagaagag
     1801 gaggcgttgc atgtcatcgc ccagaaagcc gatggtgcca tgcgcgacgc cctttcattc
     1861 ttcgaccagc ttgtcagctt ttcaggcagt cagcttacgt atcagactgt taccgaacat
     1921 cttaacatcc tggatcatgc ctattatttc aggatcatgg accaggcgct cagccaggat
     1981 accagcggca ttttactgac cctggatgag atcatcagcc ggggatttga tggccagcat
     2041 ttcctgatcg gcctggggga acatctccgg aacctgctgg tatgccagga cccaaaaacg
     2101 gtgaccctga tggatataag ctccaatctg cagcagaagt atgcggaaca agccggaaaa
     2161 tgttcaccgg cttttttgct ccaggcgctg gagattcata accagtacga tcaaagctac
     2221 aaaacagcca ataacaaacg gctgcagctt gaactggtca tgctgctgat cagtgctgct
     2281 gcagtaaaat ccgggtctcc ggaaccggaa aaaaagattt cattgattcc gcctccggca
     2341 gatccaaaaa aaccgggccc ggaacccggg attcctttaa cgccaaagcc cgaagaaaat
     2401 aagactgttg cgccccctgt tgctgcggtt cccaagccat cggcagcact gtcggctagc
     2461 cgcaccatct ccatcaaacc cgaaaatcat acgccgaaaa aggttggagc aaccggtgaa
     2521 gaaacgcctg caggaccggt taatcagaaa gaattcacac aagagaagct ggaaaaagtc
     2581 tggctctatt acacggaaag cattgcgggt cagtatccca atttttacag cattctatcg
     2641 acccggaaac ctgttctgaa ggaaaatttc ctgatctatc tccagttgga caataagact
     2701 caggagatca ccttaaaaga ccgccgcgcc gatctcctgg actttttacg caacgaatta
     2761 ggaaatcagt ccgttcagat ggaatccgtt gtcattgaga cagcaacgca atcaaaaccc
     2821 tatacgccgg atgagaaatt ccggtcgatg gtggaaaaga atcccgaatt aaaggtttta
     2881 aaggaaggtc ttgacctgga gatcgagttc tgaagaagga gtcttaaaaa gacatgttct
     2941 tcagaacccg tccaggtatg atcattattc cggatgaacc gggtttgaga tattatcctc
     3001 catatttgca ggcatcaaca cggcttcgat aatattatcg ccaaagaatt gctgggcagc
     3061 cgtttttaat gatccgaccg acatgctgtt cacataatct tcatacttca tatattcatc
     3121 caggccggtg ccatagtaat ccttattttt aatgattccg agccaatagt tgttttgcct
     3181 cagattttcc tggtggactt tgatcttgtt ctctttcacg ccgtgcaggt ctttctccgt
     3241 cgggccatcc gttttcagct tgccaatttc atcataaacg atctgtttaa gtttgccggc
     3301 attttgcgga tcacagtcaa agaatatagt aactgaataa ttttcgtaag gatatttgtc
     3361 catgctctca taaaccgcgg ccccataagt tccgctctgc tcttcgcgga tggtttccac
     3421 aaatcgcaca tcgagaatat cgcaaagcgc cgacaggttc agcctggatt gaaaatcatc
     3481 ataatcaaaa gtaccggtat atgttaaata gacggtacct ttcgggactt tcatttcacg
     3541 ttcgatcaca cgttccactt tgcctgcagg cggacggata ttattgtctt tgaatgtttc
     3601 ttcacgtgag actttcggca gtccgccgag atatttttcg atcaggggtt tagcttcgac
     3661 gggatcgata tttccgacga aatagaaggt aaaactgccc ggatcgccaa agcgggtttt
     3721 gaagatggag cgggccttct taagattggt ctcgtcgagt atggcagagg tcaatggccg
     3781 cacgcgggga tgatggttgg ccatcaggac cgtcacggta tcctgaaggg ctgattcggg
     3841 ttcgagcgct ttattatcca gcatagcctt cattctctta ataaaagcat taaaggccac
     3901 ctcatctgtt ctgggagccg taaaatagag attgaccatt tggagcatcg tttccaggtc
     3961 tttcacagag caagaacctt caagaccctc tgagctcccc ttgatcaccg gccttacgct
     4021 cgcgatcacc cccgtcagtt ttttatccag ggcaatccgg tcgaactcgc ccaatccgct
     4081 ttccaatgaa atatcggaag caaacatagc cgacatcagg tccttggttt catacagtga
     4141 ggatccgcca aaactataag cgctcatcag gatctcatca tctttaaaat cggttgtttt
     4201 catcacggct tttaccccgt tctcaaaggt ccaggtggtt gttcccaatg ttttatcctt
     4261 tcctttctta gccacctttg caggtgtagg ttctgcagcc atcagcggct tatcggtcac
     4321 tttatccacg taagccgtta tgggttccgt ttcaacatcg gtgatgatat ccctgacttc
     4381 ctgttccgaa ggcactttaa tatcgggatt gtcgggggcc atcagtgaaa cgaccctgtt
     4441 tttatcaatt atccacttac ccgcctgggc attgacctcc tccagggtaa ttcccggaag
     4501 catagcattg gtaaaggcaa aatcataagc aacgcccggg gttggctcct gttccaggaa
     4561 gtgtgagaaa tactgccaga catactggtc
//
LOCUS       DAOQXS010000216         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19168_length_4586_cov_4.233503, whole genome shotgun sequence.
ACCESSION   DAOQXS010000216 DAOQXS010000000
VERSION     DAOQXS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19168_length_4586_cov_4.233503"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKM34_06945"
     CDS             <1..736
                     /locus_tag="PKM34_06945"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNQ83363.1"
                     /translation="LLINSTVARNYTGSAVSTTESCLPNMINNIIAYNEGYGVESPLG
                     CIRYFGYNDVSHNTAGNFYNPPEWIGENITVNENGDSCDAYHNILVDPLFKNLQENDF
                     HLGLNSPCIDAGLNDSVVNSTDYDGNPRILFCANSLTVDMGAYETLPVGIAHIRQDEN
                     EGLIIAPNPASEWIQIIIPGICLNERCILFIFNSSGVMIEQLFYENMADNFKINVRNY
                     PPGLYLAALWKDGSVVVQGKFFVRPW"
     gene            910..2325
                     /locus_tag="PKM34_06950"
     CDS             910..2325
                     /locus_tag="PKM34_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ83364.1"
                     /translation="MFLICLSIQAQQTPPSPCGPVPTAQQLAWHELETYAFIHFTTNT
                     FTDKEWGYGDESPEMFNPTDFDAEQWVKVLKEAGMQALILTCKHHDGFCLWPSRYTEH
                     SVKNSSFMDGKGDVVKAVSDACRKYGLKFGIYLSPWDRNQPTYGSAAYIDYYRNQLGE
                     LLTAYGPVFEIWFDGANGGEGFYGGARENRQIDRRTYYQWPLTIDMARQVQPDVLFFS
                     DAGPDIRWCGNESGFAGETNWCLINPDTLYAGKSGIEGLLNSGSADGTRWIPAEVDVS
                     IRPGWFYHPSQDSQVKSPEKLFEIYLSSVGRGSNLLLNIPPDRRGLLHENDVKSMLGF
                     KKLRDEAFAYNLAFNARVTASSFRGSSPQFNPANLVDNNKETFWATDNTITTSDIVID
                     LGIEKCIRYLVLSEYIRLGQRVRSFEIEVLKDKDWVTVATGTTIGYKRIISLDPVFAQ
                     KIRISITGSKACPVLSEIEVY"
     gene            complement(2373..2648)
                     /locus_tag="PKM34_06955"
     CDS             complement(2373..2648)
                     /locus_tag="PKM34_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83365.1"
                     /translation="MKFIYYDELVSGQEEIIFPDSLSREDQKKLITERLKKGPDDTVQ
                     SISGMPYTRDDLIHAIENDEDMGNMTMEAELIMLRDLLRLIAGQRAK"
     gene            complement(2789..3916)
                     /locus_tag="PKM34_06960"
     CDS             complement(2789..3916)
                     /locus_tag="PKM34_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83366.1"
                     /translation="MKIGKLNFKDYAAKKPVLLSPSGKFLTIKEVTATPKFEFGSLHT
                     LSGESQAKLALERYKLEPDFKLGIFEQGVMTKADVMKHIQDGTEFGQLAVQVEMQYCN
                     ELMQSLKMTTVPAFPKLPDKPLPAVPEWKQIKRCFWFRIKNIVLFCENTTDAVTTPFA
                     LYRIANVHSVFAARGFNIVALTGTNVTRTNFEATAKKPLTVYLSGIGHGNYDVYTGHA
                     GGIILKVGAYDPAEVKGKAIHFLSCRTAARLGPDTVAKGAKCYAGYDENFTFVWDNPA
                     TPINEVDLFKKSDSIFDIYMANGFTAQQAYNAAIATFNACIALVPGTAAASWLSYDRD
                     HFRLLGDPNTRISPYRFVRICLPLKTPLHEEMLAEVGELMD"
BASE COUNT         1167 a         1021 c         1132 g         1266 t
ORIGIN      
        1 gttgttgata aattcaaccg ttgcaagaaa ttacacagga agtgcagtat caacaactga
       61 atcgtgcctt ccgaatatga tcaataatat tatcgcttat aatgaaggat atggcgtgga
      121 atcacctctg ggatgtatca ggtatttcgg gtacaatgat gtttcacata atacagccgg
      181 gaatttttat aatccgccgg aatggattgg cgagaacatc actgtgaatg agaatgggga
      241 ttcatgtgat gcctatcata atattctcgt ggacccgctg tttaagaatc tccaggaaaa
      301 cgattttcac ttgggtttga atagcccctg cattgatgcc ggacttaatg attcagtggt
      361 taattcaacg gattatgacg gaaatcctag aatactgttc tgtgccaata gcttaaccgt
      421 agatatgggt gcctatgaaa ctttacctgt tggaattgcg cacatacgcc aggatgaaaa
      481 tgaaggactg atcattgcgc ctaatcccgc cagtgagtgg attcagatca ttattccggg
      541 aatatgtctg aatgaaagat gtattctatt catttttaat tccagtgggg tgatgataga
      601 acagcttttt tatgagaata tggcagacaa tttcaagata aatgtccgca attatcctcc
      661 cggtttgtac ctggcagcat tatggaaaga cggatccgta gtagtgcagg gtaaattttt
      721 tgtgagacct tggtaatgtt ttactatagg aggatggccc gataggactg taaaacggta
      781 taaatagttg agaatagcat tgcccgtagg gcataaagta ttatggcaga gatggttgcg
      841 aataaggact tttaccctgt aggggttaaa caaattatac ggttggcgcg cgattgtttc
      901 gcgaaccttt tgtttctgat ttgtctcagc attcaggccc agcaaacccc tccatcaccc
      961 tgcggcccgg ttcccactgc ccagcaactg gcctggcatg agcttgagac gtatgctttc
     1021 atccatttta caaccaatac ttttacagat aaagaatggg gttacggcga tgaaagcccg
     1081 gaaatgttca atcccacgga ttttgatgcg gagcaatggg taaaggtact gaaggaagcc
     1141 gggatgcaag ccctgatcct tacctgcaag caccacgacg gcttctgcct ctggccttcc
     1201 agatacaccg aacattcggt taaaaacagt tcatttatgg acggcaaagg cgacgtggta
     1261 aaagcagtct cagatgcatg ccggaaatac gggctgaaat tcgggatcta tctctcgccc
     1321 tgggaccgga accagccgac atacggttcg gcggcataca tcgattacta ccggaaccag
     1381 cttggcgaac tgttaacggc ttatggccct gtattcgaga tatggttcga cggagccaac
     1441 gggggagaag gcttttacgg cggcgcaaga gagaaccggc aaatcgaccg caggacttac
     1501 taccaatggc ctctgacgat cgatatggca aggcaggtcc agccggatgt gctgtttttc
     1561 agcgatgccg gccccgatat ccgctggtgc ggaaatgagt cgggtttcgc cggcgaaacc
     1621 aactggtgcc tgatcaatcc cgacacactg tatgcaggga aaagtggcat tgaagggttg
     1681 ttgaacagcg gcagcgcaga cggcacccgg tggatacccg cagaggtcga tgtctccatc
     1741 cggcccggct ggttttatca tccatcccag gattcgcagg tgaaatcccc ggaaaagctg
     1801 tttgagatct atctcagctc ggtgggccgg ggctcaaacc tgctgctgaa tatccctccc
     1861 gatcgtcgtg gattgcttca cgagaacgac gtgaaatcca tgctgggttt taaaaagcta
     1921 cgggatgaag cctttgctta caaccttgcc tttaatgccc gggtcactgc cagctcattc
     1981 cggggtagtt ccccccaatt caaccctgcc aacctggtcg ataacaataa ggaaaccttt
     2041 tgggccaccg acaatactat cacaacttct gacattgtca ttgatcttgg tattgagaaa
     2101 tgcatccgtt atcttgtcct tagtgaatat atccggctcg ggcagcgggt gagatcattt
     2161 gagattgaag tgttgaagga taaggattgg gtcactgtgg ccaccggaac aacaataggc
     2221 tataagcgga tcatcagcct tgatcctgtt tttgcacaga agatccggat cagcattacc
     2281 ggttccaaag cctgccccgt actgtctgaa atcgaggttt attgaaggtc gttagctcgc
     2341 tgcgcccgcg tcatttggtg gtgatttagg aatcatttcg ctctctgtcc cgcaattaga
     2401 cgcaggaggt cgcggagcat gatcaattcg gcttccatag tcatattccc catatcttca
     2461 tcgttttcaa tcgcatggat caggtcatca cgggtgtaag gcatcccgct gatcgactgg
     2521 actgtatcat ccgggccttt tttcagcctt tcggtgatca gttttttctg atcttcccgc
     2581 gaaagggaat caggaaagat tatctcctcc tgtcctgaaa ccaattcatc ataataaatg
     2641 aatttcatcc ggtgaggatc atagcataag ggttgctttg ccaaaaaatt gttcataact
     2701 cgatagtatc caatattttt ttctggtgaa agggatcacc gcctggcggc ggtgatcccg
     2761 tgatcttgtc ataaaagtaa tcaaattttc aatccatcag ctctccgact tccgccagca
     2821 tttcttcatg caacggggtt ttcagcggca gacagattct cacaaagcgg tacggcgata
     2881 tccgggtgtt cgggtcgccc agcaaccgga aatgatcgcg atcgtacgac agccagctgg
     2941 ccgctgccgt cccgggaacc agggcgatac aggcattgaa agtggcaatg gctgcgttgt
     3001 aggcttgttg cgctgtgaaa ccgttagcca tgtaaatgtc gaaaatgctg tcggatttct
     3061 tgaagagatc gacctcattg atcggcgtgg cggggttatc ccagacaaag gtgaagtttt
     3121 cgtcgtaccc ggcatagcac tttgcgcctt tggcaaccgt gtcgggcccc agtctggcag
     3181 cagtgcggca gctcaggaaa tggatggcct tcccttttac ttctgcggga tcataagctc
     3241 ccaccttcag gatgattcca ccggcatgac cggtatagac atcgtaattg ccatgcccga
     3301 taccgctcag atagaccgtc aatggctttt tagccgtagc ttcgaaattg gtccgggtga
     3361 cgttggtgcc ggtcagggcg acaatattga atcccctggc agcaaatacg ctgtggacat
     3421 tcgcaatccg gtatagcgca aaaggagtgg tcaccgcatc ggttgtgttt tcgcaaaaca
     3481 aaacaatgtt tttgatccgg aaccagaaac acctttttat ctgcttccat tcgggaacgg
     3541 ccggaagcgg tttgtcgggg agtttgggaa aagcgggaac tgtcgtcatt ttcaatgatt
     3601 gcatcagttc gttgcagtac tgcatttcga cctgcacggc cagctggcca aactcggtgc
     3661 cgtcctggat atgcttcatt acatccgctt tcgtcatcac cccctgttcg aaaataccca
     3721 gtttaaaatc aggttccagc ttgtaccttt ccagtgccag ttttgcctgc gattccccgg
     3781 atagggtgtg aagcgatccg aattcaaatt ttggtgttgc cgtcacttct ttgatggtta
     3841 aaaacttgcc ggaaggagaa agcaggaccg gtttttttgc tgcatagtcc ttgaaattta
     3901 attttccgat tttcataatt aattgatgtt aagattttcc tactctgtta gggcttttcg
     3961 gttacgccct cgttttttca tgaaactgag ccggcaaagt ttatcatgcc agggggtcag
     4021 cctgttgagt aaccaatctt taaggtttac agtgaataaa tatcaatgtc aacaacatgt
     4081 ataatatgtt tgtaattatt ttcagaatca atagaatcaa agaaaatttc ggatgccggt
     4141 ttagtgattg gcagtcgggg ggaagtttca ggtcaacgat gccagaaggc tttgtaaaga
     4201 aataagaaag taaaacaggg gggattttac tctttcggga gtttcaactt tgccacccca
     4261 aacttacgaa atattaagca aaatgtcaag tgttcagatg gatattttta aagatgtcat
     4321 tagctcgctt cgcgagcgtc attaagtaga tattaggttg tgattttgaa ttttattttt
     4381 tgaatttgtt tggaatttgg aatttggagt ttggaatttg tttggatttt ggtgcttgga
     4441 atttggaatt tgtctcctct ttagactgcc tccaaattag cccggaccac ttatggtcag
     4501 aagaagaatc catactttag cctccgcttt ttaaaggcta aaattatgcg catcgaaaga
     4561 atctctccgg cggtcgcgca gaaaaa
//
LOCUS       DAOQXS010000217         4580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19213_length_4580_cov_4.348287, whole genome shotgun sequence.
ACCESSION   DAOQXS010000217 DAOQXS010000000
VERSION     DAOQXS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19213_length_4580_cov_4.348287"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            491..1198
                     /locus_tag="PKM34_06965"
     CDS             491..1198
                     /locus_tag="PKM34_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83367.1"
                     /translation="MTGDEIPGLINVTDFGHPLKQPYEAFTSNSAHVSKNGKWVIISS
                     HIIIDDFSDDENESELEAYAECLQVFDSNGNLFRKIFSDDAEMNDVGITDDGDYLFYD
                     YQILKDLYYDNHSNRNPGLRIINLKTMDIELDISDYDTNWVYLYPYVAGDFICIFSET
                     RKDYEDLDSAGCNSAVCYNFKKNIKYTGPIGPAFKEFWRIIDVSDEGIYFRVSSAREA
                     QPVLYRFDKDFTKEKIK"
     gene            1375..2592
                     /locus_tag="PKM34_06970"
     CDS             1375..2592
                     /locus_tag="PKM34_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83368.1"
                     /translation="MILKETIVEILHQQDEKIKAKDAGIPRQLNDNIAVGGNLILIIS
                     GIRRCGKSTLLNQIKTTTQKSCYFNFEDILATDFELSDFKKLDSIIKELYPGAYLFFD
                     EIQVVEGWENYARSAQDDGFSIIITGSNASLLSRELGTKLTGRHLTYELFPFSYAEFL
                     VYKKASPGIDSFSDYFITGGFPEYIKHSNPEILQQLLRDILNRDIIVRHGLKNSTLVG
                     KLATFLMTNAGKKFSYNSLMKTFETGSANTIIDYVSYFEDSYLLLTIPSFSYSIKDQI
                     RNPKKIYSIDHGMARVNSLSFSKDEGRILENMVFLHLRRKWKKIYYYSVKSECDFVVF
                     DRDKVEMVVQVCYELSDDNMAREISGLKEALDFFQLKTGFLISYNQEDVIEDGERKII
                     VMPAWKWMMEGKL"
     gene            2589..3599
                     /locus_tag="PKM34_06975"
     CDS             2589..3599
                     /locus_tag="PKM34_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ83369.1"
                     /translation="MNPKHYTIPIFIPQLACPFQCVFCDQEKITARGHIPGIPEMVNI
                     IEQYHFSFPKGKKHVEIGFFGGTFTGIPLEEQEKYLQVAGEYIEKGWVQGIRLSTRPD
                     YIDETRLHLLKKYHVTTIELGAQSMDDEVLALSSRGHTARDIEMASQMIIKSGFRLGL
                     QMMIGLPGDTLEKDVSTAEKIIALGAVETRIYPLLVIKDTVLEKWYHQGKYQPLTLEE
                     TIDRLKILLPMFEVSGVEVTRVGLHASIGLQTGKELVAGPYHPSIRELAMTGVWWDRL
                     EVLTAFEGTDELRITVHPSQYNFAVGYYGKNRKKLMQVIKEVKFLKDKDLNQMEFLVD
                     YR"
     gene            3655..4392
                     /locus_tag="PKM34_06980"
     CDS             3655..4392
                     /locus_tag="PKM34_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83370.1"
                     /translation="MLIIVDNKLSRGAKNILSSYGELMEIQTEGITYPAISGHPDIFF
                     CQGPGKLIVAPNLPERYFDQLRKNGIEFITGELPVGPEYPSSARYNAVATPKYLIHNF
                     RHTDVMITRTFMELEPVCVEQGYCRCNLLALKDDCFMTSDEGIEKVFMRWSLGRRRLH
                     LTNRGRHPLGPRPQGRHPYGILLEGFPHGFFGGCCGVLDDKIFINGNLDFFHEGKKVR
                     DFISSLNYEIVELHHGPLTDAGSILFC"
BASE COUNT         1316 a          926 c         1050 g         1288 t
ORIGIN      
        1 atttctactt cgattatctc cgcaagggga cctatgtctt cgaatacccg ctgaatgtta
       61 cgcagaaggg agaattttcc aacggcatca cctcgatcca gtgcctgtat gcgccggagt
      121 ttgcggccca ttcgcaggga atcagggtga aggtggaatg atttggcgat ttggcgattt
      181 ggagatttga ggatttggag atttgggggt tggacgattt tgaattaata ttgattatat
      241 gagattcctg ctatcgtttt taatccttgc tgcatctgtt ttacagggac agaatattgt
      301 caggtatgag aaaaatcatc gggatacgga actttgcaag ataacgttca ttgataccac
      361 caccaacgaa atcgtaaaaa cattctcgcc ggccgaaaaa tgtccatact ggaatcttac
      421 ttataaaagg atcggggata cgatcaatga ccgaagtgta aaatattttg atctgaaagg
      481 tattgattct ttgaccgggg atgaaattcc cggccttatt aacgtcactg actttggcca
      541 tcctttaaaa caaccttatg aagcctttac gagtaattca gctcatgttt caaaaaatgg
      601 gaaatgggta attatttcat cacatatcat tatcgatgat ttcagtgatg atgaaaatga
      661 aagcgaattg gaagcatacg ccgaatgcct gcaggtattt gattcaaatg gcaatttatt
      721 ccggaaaata ttttcagatg atgctgaaat gaatgatgtt ggtattactg acgatgggga
      781 ttaccttttc tatgattacc aaatcttgaa ggacctttat tatgataatc attcaaacag
      841 aaatcccggt ttaaggatta taaatcttaa aacgatggat atcgaactag acatctctga
      901 ttatgatacg aattgggttt acctctatcc ttatgtggcc ggagatttca tttgtatctt
      961 ctctgaaacc cgtaaagatt atgaagattt ggattcagca ggatgcaatt cagccgtatg
     1021 ttataatttt aagaagaata taaagtacac aggacctata gggcctgctt ttaaagaatt
     1081 ctggcggatt atcgatgttt cggatgaggg gatatacttt cgggtaagtt ctgccaggga
     1141 agcacaaccc gttctttaca ggtttgacaa agatttcaca aaagagaaga tcaaataact
     1201 ggcactcacc aaatcagtga aaatccgtat aatccgtgtc atccgcggtc tattaaaaac
     1261 ctgaatcctc aattagtgca tattttatga taatttttta cccaattatc attcccaatg
     1321 aataatatca tatatttgta atgaacaata atgtatatta aaaatgcata aattatgatt
     1381 ttaaaggaaa ctattgttga gatccttcac cagcaggatg agaaaataaa ggcgaaggat
     1441 gccggtatac caagacaact gaatgataac attgcagttg gtggaaatct gatattgatc
     1501 atatccggga taagacgttg tggaaagagc actttattga accagataaa aacaaccaca
     1561 cagaaatcct gttattttaa ctttgaagat atcctggcaa cggattttga attatccgat
     1621 tttaaaaagc ttgattcaat tatcaaagaa ttgtaccctg gagcttatct ttttttcgat
     1681 gaaatacagg ttgtggaagg ctgggaaaat tatgcaagat ctgcccagga tgatggtttt
     1741 tcaataatca ttacggggtc aaacgcttca ctgcttagcc gtgaattggg gacaaaacta
     1801 acaggccggc atttgacgta tgaattgttt cctttttcat atgcagagtt cctggtttat
     1861 aaaaaagcaa gcccgggcat tgattcattc agcgactatt tcatcaccgg tggctttccg
     1921 gaatatatca agcatagtaa tcctgaaatc cttcaacaat tactcaggga tatacttaac
     1981 agggatatca tcgtcaggca cgggctgaaa aacagtaccc tggtcgggaa attagcaact
     2041 ttcttaatga ccaatgccgg gaaaaagttt tcgtacaact cccttatgaa aactttcgaa
     2101 acaggctctg ccaatacgat tatcgattat gtatcctatt ttgaggattc ttatcttttg
     2161 ttgaccattc cttcatttag ttattctatt aaagatcaga tcagaaatcc aaaaaagatc
     2221 tattcaattg atcatggtat ggcccgtgtc aattccctca gtttttcaaa agatgaggga
     2281 aggatcctgg aaaacatggt ctttcttcat cttcgcagga aatggaaaaa gatttactat
     2341 tattccgtta aaagtgaatg tgattttgta gtattcgacc gcgataaagt agaaatggtt
     2401 gtacaggtat gttatgaatt atcagacgat aacatggcgc gtgaaatttc cggattgaaa
     2461 gaagcgctgg atttttttca attgaaaacc ggttttttga tcagctacaa ccaggaggat
     2521 gttattgaag atggtgagag gaaaataatt gtcatgccgg catggaaatg gatgatggaa
     2581 ggaaaattat gaacccaaaa cactacacca tccctatctt catcccgcag ctagcctgcc
     2641 cgttccagtg cgttttctgc gaccaggaga agatcacggc ccgggggcac atacccggga
     2701 taccggaaat ggtcaatatc attgagcagt atcatttctc atttcccaaa gggaagaagc
     2761 atgtggaaat agggttcttc ggagggacct ttaccgggat accgcttgag gagcaggaaa
     2821 aatacctgca ggttgcgggt gagtatattg aaaaaggatg ggtacagggc atacggcttt
     2881 ccacgcggcc tgattatata gacgaaacga gactccatct gctcaaaaag tatcacgtca
     2941 ccacgatcga actcggcgcc cagtcgatgg atgatgaggt tctggctttg tcttcacggg
     3001 gccatacggc gagggatatt gagatggcat ctcaaatgat catcaaatcc gggttccgcc
     3061 tcgggctcca gatgatgatc ggactgcccg gcgataccct ggaaaaggac gtttccaccg
     3121 ctgagaagat catcgcactg ggcgctgtgg aaacccggat ctatccgttg ctcgtcatta
     3181 aagatacggt gcttgaaaag tggtaccacc agggcaagta ccagcccctg accctggaag
     3241 aaacgattga ccggctgaag atcctgctcc ccatgtttga agtttcgggc gtggaggtaa
     3301 cccgagtcgg gctgcatgct tccatcggcc ttcagaccgg gaaagagctg gttgcagggc
     3361 cttatcatcc ctccatacgc gaactggcca tgaccggggt ctggtgggac aggctagagg
     3421 tgttgactgc ctttgaaggt acagatgagc ttcgcatcac agttcatcct tcacagtaca
     3481 attttgccgt cgggtattac ggaaaaaacc gcaaaaagct gatgcaggtc attaaagagg
     3541 ttaagttcct gaaagataaa gacctgaatc agatggagtt cctggtggat tacaggtaga
     3601 ggttcaattg ttcaattgtt caattgttaa actgttatat tgtccaactg agaaatgttg
     3661 atcattgttg ataataaact atcccgtgga gcgaagaata tactttcttc ctacggagag
     3721 ctcatggaaa tacaaaccga aggcatcacc tatcctgcta tttctggtca ccccgatatt
     3781 ttcttctgcc aggggccggg gaagttgatc gtggcgccga atctgccgga acgttatttt
     3841 gatcagctcc gtaagaacgg gatcgaattt atcaccggcg agttgccggt cggaccggag
     3901 tacccgtcgt cggcccgtta caatgctgtt gccacaccga agtacctgat ccacaatttt
     3961 cgccacaccg atgtaatgat cacacgaact ttcatggaac tggagccggt ctgtgtggag
     4021 caggggtact gccggtgcaa cttgctggcg ttgaaggatg attgtttcat gacgtcggat
     4081 gaggggattg agaaggtttt catgagatgg agtctcggaa gaaggagact tcatctcacc
     4141 aaccggggac gccatccctt gggacctcgt ccccagggac gccatcccta cgggatccta
     4201 ctcgaaggtt tcccccacgg tttcttcggc ggctgctgcg gcgtcctgga tgataaaatc
     4261 ttcataaacg gaaacctgga tttctttcac gaaggcaaaa aagtcaggga tttcatttct
     4321 tcattaaatt atgagatcgt cgaattgcat catggacccc tgacagatgc cgggagtatt
     4381 ttgttttgtt aattcatgat tatttatagc cggctgtaat gtaattctcc agatcattat
     4441 attgaccatc agacaataat ccctgtgtca attgtgatcc ttcgggcttc gccctcagga
     4501 caagctgccg gctgctgact gccggctgcc aactttttcc tatcttcgca cccaaattat
     4561 aatccaacga ctatgcttcg
//
LOCUS       DAOQXS010000218         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19260_length_4571_cov_3.630647, whole genome shotgun sequence.
ACCESSION   DAOQXS010000218 DAOQXS010000000
VERSION     DAOQXS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_4571_cov_3.630647"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PKM34_06985"
     CDS             <1..1133
                     /locus_tag="PKM34_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83371.1"
                     /translation="YMEMKRNETAFEYCNKTLSVASEIGAQPFITECNLLLGKFYYLE
                     GNYDKAREFLAAAAESVKKTNDKPMMINVLLWSSRNLLEHGKYSEAIRTAREAFDMAE
                     ELMIHAGSKDVAMVLSQAYEKNGQPGEALFWQKQYQKLSDSLNFANQHKEITRIEAKY
                     NYDKKEKENEILRNKATLQEQKLRSRTIVGIAFVTGLIFSIIIIILLVRRSRDARLLF
                     QQQQMLNLKHLEELENELDGKNRELTSKMMFLNQKNDLISRLMRRLQEIRDSDENSSE
                     EILSLVNELRSDSPQSSWKEFETQFIQVHPGFYQRLFEKHPALTSYEQRICAFLRMNL
                     NTKEISSITGRSAKSIEVARSRIRTKLELNRNENLSSYLAAI"
     gene            1436..2560
                     /locus_tag="PKM34_06990"
     CDS             1436..2560
                     /locus_tag="PKM34_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83372.1"
                     /translation="MTPLTEDIRNEKLIFRNWENALPVKKNFSGGILVTGANSFLGCH
                     VIKALQQNFQGDIHLLVRAPNDFEAVARMQQSFDRWELGQFNIRRFNIHRGDVCLSGM
                     GMNPEEYRRMCRKTRQVIHLAMTPMYHLPYHHFQQTWIPELERMISFCMDKEYPKFLH
                     YTSSYNANFFVTDEDFQSLNRNAWQSGYAGFKWVAQEAIRNASRQGLGSCIYDVPLVL
                     GSERKGICPSHYSIWIILDIFLKTGYFFPFSFRIIPVDMLAEMIVLNADAAGIGRASG
                     FIRPFLEEPVTDSLFARTAANLLGLKEATLETVRQNCINKLRFDFMMPVNFYSLLEKV
                     NGLPPVFPEWYDRGVLPVTPMVFMSNLNRIMSVKKEMIKT"
     gene            2571..>4571
                     /locus_tag="PKM34_06995"
     CDS             2571..>4571
                     /locus_tag="PKM34_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83373.1"
                     /translation="MKPKSQKFASRFTLHASLLFLALCPVLLATGQNHKWYYNYNTGK
                     SEEGKDIVCGSDGYIYIAGTEYDEADYDIIVIKLDKSGNQQWVYSYEGQPDKTMVVAE
                     IQAGSDGNIYVCGTANNAEDNNKFLVISINPEGGLRWEYIYDVDGNYPSEAFSVAYGG
                     NGMVYAAGKANYDFLVAGINAGSGEQEWIYWFDGGCPYALCDDQASAITVGNDGNIYA
                     AGYTHEATEPQLTILSLTPEGSKNWKYLFPFSEGRTWATDIVYGDDGRIYASGLIYSN
                     IAVICVDATGQYQWNCNVDGPGPEPYWGETCYELIYGFDNNIYVTGRAGGRDNQVDTD
                     MDVAVIKVDLQGSPVWFYRYEGLYGDHDMAFSITQTPDTNVHVAGYFCGLLAEAGTIS
                     IDHRTGRDLWVMRYVGPAIDMDVAYAITSDEDGYLYVTGYDYKANRLHDVYVWKLQPP
                     KNTDGYYNLEGYATWGEGHAVLETADNGFIIAGYQGTSLTSSTYNMRLIKTDINGDTL
                     WTRNYGGNNEERTFDVAICPDNGYILTGFTKSYGAGGKDLYIVKTDEQGNMQWEKTYG
                     VETDEEGYSIVTAADGGYFIAGKTYQYDGSGDLWFMKVNSQGDSLWTKRYGGDRRDEV
                     GEVHRTADGGYIFAGTRGHALTLGYITNIYAIRFNADGDTLWT"
BASE COUNT         1252 a         1070 c         1175 g         1074 t
ORIGIN      
        1 tttacatgga gatgaaacgg aatgagaccg cttttgaata ctgcaacaag actttatccg
       61 tagcaagtga gatcggagcc cagccattca ttaccgaatg caacctttta ctgggaaagt
      121 tttactacct ggaaggaaat tacgataaag cccgggaatt tttagcggcg gcggcggaat
      181 cggtcaaaaa aaccaatgat aaaccaatga tgatcaatgt tttgctttgg agttccagaa
      241 atttactgga acatgggaaa tattcggaag ccatccggac ggcacgggaa gcattcgaca
      301 tggcggaaga actcatgatt catgccggca gcaaagatgt ggcaatggtt ttatcacagg
      361 cttatgaaaa gaatggtcaa ccgggtgaag cgctattctg gcaaaaacag tatcagaaat
      421 tatccgatag cctgaatttc gccaatcagc ataaagagat cacccggatc gaggcaaaat
      481 ataattacga taaaaaagaa aaagaaaatg agattctccg gaacaaggcc actttgcagg
      541 agcaaaaact gagaagcagg acaatagtcg gcattgcttt cgttacagga ttgatattca
      601 gcattataat tattatcttg ttggttagaa ggagccggga tgcccggtta ctttttcagc
      661 agcagcagat gttgaatctg aaacatcttg aagaactgga gaatgagctg gatggtaaaa
      721 acagggaact aacctcaaaa atgatgttcc ttaaccagaa gaatgatttg atctccaggc
      781 ttatgcgacg gttgcaggag attcgcgatt ctgatgaaaa ttcatcggaa gagatcctgt
      841 cgctggttaa tgaacttcgt tccgactcac ctcaaagcag ctggaaagaa ttcgaaacgc
      901 agttcatcca ggtgcatccg ggattttacc agcgtttgtt tgagaagcat ccggcgctga
      961 cttcgtatga acaacgcatt tgtgcttttc tgaggatgaa cctgaataca aaggagatct
     1021 cttcaataac cggaagatcc gccaaaagta tcgaggtggc ccgaagccgc atccgcacaa
     1081 aacttgaact gaacaggaac gaaaacctta gcagttacct ggcagctatt tgatccttga
     1141 actaacgggt cccaactttg taaggaggct gtttcaaaag tctgatattt cgatttgatt
     1201 gcctgaaaac aggacgtgct ccaacgacta aagctgttgg ttcctgggaa tcttagaagg
     1261 tttgaaccga cggcttcagt cgtcggacta cctgaaaaaa gtgaatatat agacttttga
     1321 gacaacctca ggcgagtgtt tttgtaaggt gccgtgagtg gaaacgtaac ctgaaatatt
     1381 tgcattgacc tttctggcgg ttgtatcttt ataacagcca atcaaatcat ctgacatgac
     1441 tcctcttact gaagacatcc gcaatgaaaa actgatattc cgcaactggg agaatgcttt
     1501 gcctgttaaa aagaactttt cgggagggat actggtgacc ggagccaact cctttctggg
     1561 atgccatgtt atcaaagctt tacagcagaa cttccaggga gatatccatc tccttgtccg
     1621 tgcgcccaat gattttgaag ccgtcgcccg gatgcagcaa tccttcgacc ggtgggaact
     1681 ggggcagttc aatatccgtc gttttaacat ccatcggggt gatgtatgtt tgagcggaat
     1741 gggaatgaat ccggaagagt acaggcgaat gtgcaggaaa acacgacagg taatccatct
     1801 ggctatgacc ccgatgtacc acctgcccta tcatcatttc cagcaaacat ggattccgga
     1861 gcttgagcgg atgatttcct tttgcatgga caaagaatat cccaaatttc tgcactatac
     1921 ttcttcttat aacgctaatt tttttgtgac cgatgaggat tttcagtcct tgaacaggaa
     1981 tgcctggcaa tcgggttatg ccgggttcaa atgggtagcg caggaagcca tccggaatgc
     2041 ctcacggcag ggcctcggtt catgtattta cgatgtcccg ctggtgcttg gttctgagcg
     2101 aaagggcatc tgcccgtcgc actactccat ctggatcatc ctggatattt ttctgaagac
     2161 gggttatttt ttcccgttca gtttccggat catcccggtg gacatgctgg cggagatgat
     2221 tgttctgaat gctgatgctg ccggcattgg aagggcatcc ggttttatca ggcctttcct
     2281 ggaagaacct gtcaccgaca gcttatttgc acggacggca gcaaacctgc tggggcttaa
     2341 agaagcaacg cttgaaacag tcaggcagaa ttgtatcaat aaactccgct tcgatttcat
     2401 gatgccggta aacttttatt cattgcttga aaaagtgaac gggttgccgc cggttttccc
     2461 ggaatggtat gacagaggcg ttttaccggt gacacccatg gtatttatga gcaacctgaa
     2521 caggattatg tcggtgaaaa aggaaatgat caaaacataa aatatcggcc atgaaaccaa
     2581 aaagtcagaa attcgcttca cgctttacgc ttcacgcttc actgctgttc ttagccctat
     2641 gccctgtgct cttagccacg ggccagaacc acaaatggta ttataattac aacactggca
     2701 aaagtgagga aggtaaagat atcgtctgtg gttccgatgg atacatttat attgccggta
     2761 ctgaatacga tgaagctgat tatgacatca tcgtgatcaa attagacaaa agtggtaacc
     2821 agcaatgggt ttacagctac gaaggccaac cggataaaac catggtagtt gccgagatcc
     2881 aggcaggcag cgacggcaac atctatgtat gcgggactgc caataacgcg gaggacaata
     2941 ataaattcct ggtgatcagc ataaatccgg aaggaggctt gcgatgggaa tacatttatg
     3001 atgtggatgg aaactatccc agcgaggcgt tttctgtcgc atacggcggc aacgggatgg
     3061 tttatgcggc cgggaaggcc aactatgatt tccttgtggc cgggatcaat gccgggagtg
     3121 gggagcagga atggatctac tggttcgacg gcgggtgccc gtatgcttta tgcgacgacc
     3181 aggcttcggc catcaccgtg ggtaatgatg gaaatattta tgcagcgggc tacactcatg
     3241 aggccaccga accacaactc accatcctca gcctaacacc agaaggaagc aagaactgga
     3301 aatacttgtt cccattttcg gaaggcagga cctgggcaac ggacatcgtt tatggggacg
     3361 acggccggat ctacgccagc ggcctgatct attcgaacat cgccgtgatt tgcgttgacg
     3421 ccaccggcca gtaccagtgg aactgcaatg tcgacggccc cgggcccgag ccatactggg
     3481 gagaaacctg ctatgagctg atctatggct tcgataacaa catctatgtc accgggcgtg
     3541 ccggcggccg cgacaaccag gtggataccg atatggatgt tgcagtgata aaggtggacc
     3601 ttcagggcag ccccgtctgg ttctaccgct atgaaggatt gtacggcgat catgacatgg
     3661 ccttttccat cacccaaacg cccgacacca acgtgcatgt ggccggctac ttctgcggcc
     3721 tgctggccga ggccggcacc atcagcatcg accaccggac gggacgcgac ctctgggtca
     3781 tgcgttacgt cggcccggcc atcgacatgg atgtggccta tgctatcacc tccgacgagg
     3841 acgggtacct ctatgtgaca ggatatgatt acaaagccaa ccgacttcat gatgtgtacg
     3901 tctggaaact gcaacccccg aaaaacaccg atggctatta caatctcgaa ggctatgcca
     3961 cctggggcga aggccatgcc gtgctcgaaa cagctgataa tggatttatc atcgcaggtt
     4021 accagggaac ctcccttaca tcctccacct acaacatgcg gctcatcaag accgatatca
     4081 acggggatac cctttggacc cgcaattacg gcggcaacaa cgaagaaagg acgttcgatg
     4141 tggcgatctg cccggacaac ggctacatcc tcacaggctt taccaaatca tacggggccg
     4201 gaggaaaaga cctttacatt gtcaagacag acgagcaagg caatatgcaa tgggaaaaga
     4261 cctatggtgt cgaaaccgac gaagagggct attccattgt cactgccgcc gacggcggct
     4321 atttcattgc cgggaagacc taccagtatg acggttcggg cgacctgtgg ttcatgaagg
     4381 tcaattccca gggcgacagc ctgtggacga aaagatacgg cggcgaccgc cgcgatgagg
     4441 tgggcgaagt gcaccggacg gccgatggtg gatacatttt cgccggcacc cgggggcatg
     4501 ccctcacgtt gggatacatc accaatattt acgcaatccg gttcaacgcc gatggcgata
     4561 cgctctggac c
//
LOCUS       DAOQXS010000219         4544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19404_length_4544_cov_3.808866, whole genome shotgun sequence.
ACCESSION   DAOQXS010000219 DAOQXS010000000
VERSION     DAOQXS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19404_length_4544_cov_3.808866"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKM34_07000"
     CDS             <1..316
                     /locus_tag="PKM34_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83374.1"
                     /translation="KYGAAEMVDPRPFTVGKLSTTFKIYPNIGTLLPAMGYGEQQLKD
                     LEETINATDCDSVVIGTPIDLNRIIKINKPNTRVYYDLQEIGDPDLNSILDEFVKKHG
                     LK"
     gene            341..1249
                     /gene="pyrB"
                     /locus_tag="PKM34_07005"
     CDS             341..1249
                     /gene="pyrB"
                     /locus_tag="PKM34_07005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNQ83375.1"
                     /translation="MKHKSLVSIDDYSKEEQIQILDLAEGFEAKPVQNILNGYVVATL
                     FFEPSTRTRLSFESAASRLGAQVIGFSEASSSSVSKGESLNDTILTVSNYSDIIVMRH
                     PREGSARYASEVSPVPIINAGDGANQHPTQTLLDMYSIRKTQGRLDNLQIAFVGDLKY
                     GRTVHSLTFALCNFNAAFHLVSPEELKLPSYVKRKIKDNKLDYFQYTDMNEVIPQVDI
                     LYMTRIQKERFSDPIEYERVKNAYVLRNEMLQGAKKSMRVLHPLPRVNEIEKDVDPNP
                     MAYYFQQAQNGVYVRQALLASILGVC"
     gene            1287..1754
                     /gene="pyrI"
                     /locus_tag="PKM34_07010"
     CDS             1287..1754
                     /gene="pyrI"
                     /locus_tag="PKM34_07010"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNQ83376.1"
                     /translation="MEEVKTRTELQVSAIENGTVIDHIPPKSVFQVVKMLGMENCKEQ
                     VLFGTNLESKKYGRKGIIKVANLFFQSDEINKIALVAPSATLIVIRNYQVVEKRKVEI
                     PDQIFRFVRCFNPNCITNHEKITTKFNVIDKEDLKLECIYCEKVTGKNNLEFI"
     gene            1968..2624
                     /locus_tag="PKM34_07015"
     CDS             1968..2624
                     /locus_tag="PKM34_07015"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNQ83377.1"
                     /translation="MKNQKTTRNKPEPAKSTGKSVKGTNQPKKGNAAGPKKPLFSKEL
                     LLKLMPLIKAFALWFLLVLIVHIPFIKDEFRNMFVGFTTNSTVLLSKILFIPIQKLGF
                     STISLDGFTMDIVVECTAYNFYLFAIALSVFGNWSLKHKLINLLIFFGIIFLTNNLRF
                     FAMGYIGRYYPELFDTTHDYVWNILFGFMIFGVWAWRDKKNNPDFNPKDPDHATEEVR
                     "
     gene            2602..3123
                     /locus_tag="PKM34_07020"
     CDS             2602..3123
                     /locus_tag="PKM34_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83378.1"
                     /translation="MRQKKYVNILIVIGASAILLLLWFNGFSWLYAQMLRIGANILLV
                     FSGDTSISLKMVDNAPTFVVDTIINGKKGTYPQKADLILLPFIMILTWQILLFFNLPA
                     KKAWRSAAENIITFFIIQLVYVLLLTGYHNSSGVKFIYDLLLDSFYIIALFLVVKDAF
                     KYELIGLTKKDKQ"
     gene            3196..3876
                     /locus_tag="PKM34_07025"
     CDS             3196..3876
                     /locus_tag="PKM34_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83379.1"
                     /translation="MPDYITRLIAGGESQTLDFKFEVSDFRKIARTLVAFSNTDGGRL
                     LVGVKDNGSIAGVRSEEEYYMIEGAARLYCRPEIMFSVKEWQADRKKILEVIIDRGKE
                     VPYKAMDDEGQWTAYIRQGDQNFKASRIRQQAWIREKSGKPLLVQFRQGEQFLIRYLE
                     RNGGITLARFRRLAGLSQREAEAILTDFLMLGIIEETYSGFGALFILSPDYKWIIGHI
                     NSNDMAGY"
     gene            4244..>4544
                     /locus_tag="PKM34_07030"
     rRNA            4244..>4544
                     /locus_tag="PKM34_07030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1326 a          950 c         1056 g         1212 t
ORIGIN      
        1 gaagtacgga gccgctgaaa tggtcgaccc gaggcccttt actgttggca aactaagtac
       61 aacattcaaa atctatccca atatcggcac attattaccg gctatgggct acggagagca
      121 gcaacttaaa gacctcgagg aaacgattaa tgctaccgac tgcgattcag tagtgatcgg
      181 caccccgatc gatctgaacc gtatcatcaa gatcaacaag ccgaataccc gcgtttatta
      241 cgatctgcag gaaatcggtg acccggacct gaattcgatc ctcgatgaat ttgtaaaaaa
      301 acacggtctg aaatagcatt taattcataa atatcaatcc atgaagcaca agagcctggt
      361 ctccatcgac gattacagca aagaagaaca aattcagatc cttgatctgg cagaaggatt
      421 tgaagcgaag ccggtccaga acatcctgaa tgggtatgtg gttgccacac tatttttcga
      481 accatcaacc cgtacccggc ttagttttga aagtgccgca tcacgtctag gagcccaggt
      541 tatcggcttc tcggaagctt cttcttccag cgtatccaaa ggggagtccc tgaacgatac
      601 gatcctgacg gtcagcaatt attctgatat tattgtgatg aggcatcccc gggaaggcag
      661 tgcacgatac gccagcgaag tcagcccggt acccattatc aatgcaggcg acggcgccaa
      721 ccagcacccg acccagacat tgctcgatat gtattcgatc cgtaaaaccc agggaagact
      781 ggataacctt cagatcgctt tcgttggcga tttgaaatac ggcaggaccg ttcattcgct
      841 gacttttgcc ctttgcaatt tcaacgctgc tttccacctg gtgagcccgg aagaattaaa
      901 gctcccgagt tatgtcaaaa ggaagatcaa ggataataag ctcgattatt ttcagtatac
      961 cgatatgaat gaggtgatcc ctcaggtcga tatcctttac atgacccgca tccagaaaga
     1021 aaggttctcc gatccgatag agtacgaacg ggtgaaaaac gcgtatgtat tgcgaaacga
     1081 gatgctccag ggcgcaaaga aaagcatgag ggtattgcat ccgctgcctc gggtcaatga
     1141 aatagaaaag gatgtggatc cgaacccgat ggcctattat ttccagcagg cacagaacgg
     1201 ggtatatgta aggcaggctt tgctggcatc gattctggga gtttgttaat attgaaaatt
     1261 gaatgttgaa agttgaatgt tgaatgatgg aagaggtaaa aacgcgaaca gagttacagg
     1321 tcagtgccat tgagaatggt actgtgatcg atcacatccc gccgaagagc gtgttccagg
     1381 tcgtgaagat gctgggaatg gaaaactgta aggaacaggt tctttttggg actaacctgg
     1441 aaagtaaaaa atacggacgg aaagggatta taaaagtagc caacctgttc ttccaaagtg
     1501 atgaaatcaa caaaatcgca ctggtcgcac cttcggccac tttaatcgtc atccgcaatt
     1561 accaggtggt tgagaagaga aaagttgaaa ttcccgacca gatctttcgt tttgtcaggt
     1621 gcttcaatcc taactgcatc accaatcatg aaaaaataac gacaaaattc aatgtgatcg
     1681 ataaagaaga cctgaaactg gaatgcatct attgcgagaa agtgacagga aaaaacaacc
     1741 tggaattcat ttgagaaatt ccgttacata aggtattaaa aggcgcccgg gccggtttgg
     1801 aataaattcc gccggcccgg tacgcttttt taaaccacca tccggtattt atcattttta
     1861 ataaaaaagg taatacgcga taaacattaa aacctcgata taattacaaa atccctcatt
     1921 gtaattctgc attcttttat atttttacac aacaaaatta ttttgccttg aaaaaccaaa
     1981 aaaccaccag gaataagccg gaaccggcaa aatcgacggg caaatccgtc aagggaacga
     2041 accaaccgaa aaagggtaat gccgccgggc ccaagaaacc cctgttcagc aaagaactcc
     2101 tgctaaaact tatgccgctg atcaaggcct ttgccctttg gtttttactt gtcctgatcg
     2161 ttcatattcc ttttattaag gatgaattca ggaatatgtt cgtgggattt acaacaaatt
     2221 caacggtact tttatcgaaa atcctgttca ttcccatcca gaaactggga ttctccacta
     2281 tttcactgga tggttttacc atggatattg tggtcgaatg cacggcttac aatttttacc
     2341 tgttcgccat tgccctgtcg gtattcggga attggtcgct gaaacataag ctgatcaatc
     2401 tgctgatctt tttcggcatc attttcctga ccaataacct gcgtttcttt gccatggggt
     2461 atattggaag atattatcct gaactttttg atacaaccca cgactatgta tggaatattt
     2521 tattcggatt tatgatattt ggtgtgtggg catggcgtga caaaaagaac aaccccgatt
     2581 ttaaccccaa agaccctgac catgcgacag aagaagtacg ttaatatatt gatcgtcatc
     2641 ggagcatccg ccattttgct gctgctctgg ttcaacggat tttcatggct ctatgcacag
     2701 atgctgcgga tcggtgccaa tatcctgctc gttttttcgg gcgatactag tatcagcctg
     2761 aaaatggtgg acaacgcccc tacttttgtt gtcgacacca ttataaacgg gaaaaaaggg
     2821 acctacccgc agaaggctga tctgatcctg ctgcctttta tcatgatttt gacctggcag
     2881 attctcctgt tctttaatct gcctgcgaag aaagcgtggc ggtcggctgc agaaaacatc
     2941 attacttttt tcatcattca attggtctat gttctgttgc tgaccggata tcataattca
     3001 tccggcgtca aattcatcta tgacctttta ctcgacagtt tttacattat cgctttgttc
     3061 ctggtagtta aagatgcgtt caagtatgaa ctgatcggtt tgaccaaaaa ggataaacaa
     3121 tagctgttac ccatttcata taataaggta taaataataa cctgccgaat tttcacttcc
     3181 ttgtcctcaa gctgaatgcc tgattatatt acacgattaa tagcaggagg agaaagccag
     3241 acgcttgatt tcaaatttga ggtttccgac ttcaggaaga tcgcgcgtac gctggtcgcc
     3301 ttttccaaca cggatggcgg caggttattg gttggagtga aggataacgg ttctattgcg
     3361 ggtgttcgat cggaggagga gtattatatg atcgaagggg ccgccaggct ctattgccgc
     3421 cctgagataa tgttcagtgt caaggaatgg caggccgacc ggaagaaaat cctggaggtt
     3481 atcattgaca ggggcaaaga ggttccatac aaagccatgg atgatgaggg tcagtggacg
     3541 gcatatattc gacagggaga tcagaatttc aaagccagtc gaatccggca gcaggcatgg
     3601 atcagggaga aatcgggcaa gccgcttttg gtacagtttc gccagggtga gcaatttctg
     3661 atcaggtatt tagagaggaa cgggggcata acattggccc ggtttcggcg gctggccggg
     3721 ttatcgcagc gggaggccga agcaattctt acagattttc taatgctggg catcatagaa
     3781 gagacctact cgggtttcgg ggcactgttt atcttaagtc ctgattacaa atggattatc
     3841 ggtcatatca acagcaatga tatggctggt tattaaccat tgttaatatc ttggatgatt
     3901 tttttcagat aaggtcattg ctggatgaaa aatgtttcta cctttgcacc ctcatttacg
     3961 acacgttctt taagagatag cttaaaaggc ctgtagaata agggggagcg agagttttcc
     4021 ccgggttgtt ttaaaaaaaa taatattttt ttagaatttt ttttgcgggt atcaaaaaag
     4081 gttatatctt tgcggccctt ttcgctttga aaaaagtaaa aaagggaaag acaaaagttc
     4141 tttgaaaaga ttgaagcaag cctagcggaa gcaaatagct ccgaattaat actccaataa
     4201 atggtgaata gagcaagtcc aagagattaa ataattcatt tatacaatga agagtttgat
     4261 cctggctcag gatgaacgct agcggcaggc ttaatacatg caagtcgaac ggtaaggtct
     4321 cttcggagat acacgagtgg cgcacgggtg cgtaacgcgt atgcaaccta cctttaactg
     4381 ggaaatagcc ccgagaaatc gggattaatg tcccatatta ttttctgaaa gcattttcgg
     4441 gaaatgaaag ctccggcggt tattgatggg catgcgtgac attagctagt tggtgaggta
     4501 acggctcacc aaggcaacga tgtctagggg tcctgagagg gtta
//
LOCUS       DAOQXS010000220         4530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19500_length_4530_cov_3.174078, whole genome shotgun sequence.
ACCESSION   DAOQXS010000220 DAOQXS010000000
VERSION     DAOQXS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19500_length_4530_cov_3.174078"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1421
                     /locus_tag="PKM34_07035"
     CDS             225..1421
                     /locus_tag="PKM34_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ83380.1"
                     /translation="MKILQVANKFPYPAKDGGAIATLSLSRSFAAQGHAVTVLAMNTS
                     KHFTDISRVPEDLNAKIRLIGVPVNTDIRILPLVRNFLFSRLPYNAERFVDTRFRNAL
                     EDLLKSESFDIIQLEGLYLAPYLEAIRKFSSARVVMRAHNIEHEIWERSIAGHAGIRK
                     LYIASLARRIRRMELEFLNKYDAVVPITERDYLVLQKLGCRLPAHVVPMGVNPDELVP
                     DFSRMEFPSVFHIGALDWMPNQEGLTWFLEKVWEKVLKHHPDLKFYLAGRNAPDHFKS
                     LTYPNVSYLGEVADAYDFIRSKTVMIVPILSGSGMRIKIIEGMALGKAIVTTTIGTEG
                     IATTSGENLFVADDPDEFAQIVCNLVENREKCVAIGENARKFVTEYYDNDVITRSLIL
                     FYEKLK"
     gene            1418..2581
                     /locus_tag="PKM34_07040"
     CDS             1418..2581
                     /locus_tag="PKM34_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83381.1"
                     /translation="MTIALLIFWISLLLVVHTYVFYPFILQVLSFRKKGNRMVYEAGG
                     ELPVVSVIIAAHNEESCIAEKIESIYSGTYPYDRLEVLVGSDNSDDRTVEILAALQKR
                     FPTLQFFDFRERRGKGSVVNDLVGASKGSLLVLTDANVMFDRETLFYLVRHFRNPAIG
                     LVDTNMVNMGMKKDGISYQERAYISREVRIKDMEGRLWGAMMGPFGGCYAIRREDYCQ
                     VPANFLVDDFYINMTIFEKGKLAVNDLNSIVFENIPDELKVEFRRKVRIGTGNFQNLR
                     RFWRLAFTLRFGFAFISHKIIRWFGPFLLLAALVANIYLAVFSRVYLFTLAGHLLVYL
                     LPFFDRLLGMMKMNAGPLRLATHFFSMNAALFTGFFRSLGSVRSGAWERTKRG"
     gene            complement(2514..3986)
                     /locus_tag="PKM34_07045"
     CDS             complement(2514..3986)
                     /locus_tag="PKM34_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ83382.1"
                     /translation="MKKINALVLKTYSGPLLVTFFIALFILLMQFMWMYIDDLVGKGL
                     EWYVIAELLFYASFTFVPLALPLAILLSSLMTFGNLGEHYELVAMKSAGISLRSIMKP
                     LIILSLCISAFAFYFSNNVLPVANLKFKSLLYDIRKKKLAVNIREGIFYNDMEDYVIR
                     VGKKGKDGNRIYKVMIYNHTENRGNIDVTVADSGIMESTPDGRYLIFTLYDGFNYQDI
                     VDQKDSKNQQPFQRTSFKIQQRKFDLSAFELNRTNEELFKNNYQMLNIGQLNYFIDSL
                     SRSLVERKENLARNFPRNYQLYLNNDTAYVPSTDSVTVVPGDFLSGFSTDDQIRIVSN
                     ALTSARGIKKNLDFQSDAFRVSNESLVKHKIVWHRKFTLSFACLVMFFIGAPLGAIIR
                     KGGLGLPTVISILFFILYYIISMIGEKSALKGAMEVASGMWLSSVVLLPLGFFLTLKA
                     TTDSPLMDMDVWNKALLKVSRVWSSPRRLSGQNRATGKNR"
     gene            4126..>4530
                     /locus_tag="PKM34_07050"
     CDS             4126..>4530
                     /locus_tag="PKM34_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83383.1"
                     /translation="MKKLLLIAMALIIGVVAYSQNKVQLSKEMREQTVTLTRQNDIAE
                     EIVFGNFSDPTVSNSKAAPIETLIGTTKYDLMTNSMIQNRLYLHADNTIGAVWTMGLG
                     TEPAFSDRGTGYNYYDGAQWGDAPTVRIEPERT"
BASE COUNT         1177 a         1036 c         1135 g         1182 t
ORIGIN      
        1 cgtttcgatc ggaacgtacc cgtaatggct gaataccttt ttaacagtat cgaagatgaa
       61 attccgccgg atcatctcca ccggggtgaa atcgcgggtt cctttcggga tggtaaactt
      121 ttcgcccata gtcagactcg ttacagtttt aaacttcgtc caattttagg caaaggtcgc
      181 atttttcgtt tatttttgcc ccattgcgat tataaattca gcggatgaaa atcctgcagg
      241 tagccaataa gtttccttat ccggccaagg atggcggagc aatcgccacg ttgagcctta
      301 gccggtcgtt tgccgcccag ggccatgctg tcacggtgct ggctatgaat acttcaaagc
      361 attttaccga tatcagccgg gttccggaag atttgaacgc aaaaatcagg ctgatcggtg
      421 ttccggtcaa caccgatatc cgtattttgc ccctggtaag gaatttctta ttctcccggc
      481 tcccctacaa cgccgaacgg tttgtcgata cacgcttcag gaatgcgctg gaagacttgc
      541 tgaaaagtga aagtttcgat atcattcagc ttgaaggatt gtaccttgct ccttaccttg
      601 aagcgatccg taaattttcc tcggccaggg tagtcatgcg cgcccacaac attgagcatg
      661 aaatctggga gcggagcatt gcggggcatg ccggcatcag gaaattatat atcgccagcc
      721 tggcccgtcg tatcaggcga atggaactgg aatttctcaa taaatacgat gccgtggtac
      781 cgataacgga acgggattac ctcgtgctcc aaaagctggg gtgccgtttg cccgcacatg
      841 tcgtgcccat gggagttaat ccggatgaac tggtccccga tttttcccga atggaattcc
      901 catcggtttt tcatatcggc gcactcgact ggatgccaaa ccaggagggc ctgacctggt
      961 tccttgagaa agtatgggaa aaggtgctaa agcatcatcc cgatctgaaa ttttaccttg
     1021 ccggacggaa tgccccggat cactttaaat cgctgacata tccgaatgtc agttacctgg
     1081 gtgaagtcgc cgatgcgtac gattttatcc ggtcgaaaac ggtcatgatc gtccccatcc
     1141 tttcaggcag cggcatgcgt atcaagatca tcgagggcat ggcgctgggg aaagccattg
     1201 tgacaactac catcggcacg gagggcattg ccactacttc gggcgaaaat ttgtttgttg
     1261 ccgacgaccc ggatgaattt gcgcaaatcg tttgcaacct ggtggaaaat cgggaaaagt
     1321 gtgtagcgat cggtgaaaat gcccgtaaat ttgtaaccga atattatgat aacgatgtca
     1381 taacgcgttc actgatttta ttttacgaga agttaaaatg acgatcgccc tgctcatatt
     1441 ctggatatca ctgctgctgg tcgtacacac atatgtgttt tatcccttca tcctccaggt
     1501 gttatctttc cggaaaaaag gaaaccgcat ggtatatgaa gccggaggag aactgccggt
     1561 tgtttcggtg atcatcgctg cacacaatga ggagtcgtgc attgctgaaa aaattgagag
     1621 catttatagc ggaacttacc cttacgacag gctggaagtg ctggtgggat cggataattc
     1681 ggacgaccgg acggtggaaa ttttagcggc actccaaaag aggtttccaa cccttcaatt
     1741 tttcgacttc cgggaaagga gagggaaagg aagcgtggtg aatgacctgg ttggcgcgtc
     1801 aaaaggttct ttgctcgttt taacggatgc aaatgtcatg ttcgacaggg agacgctgtt
     1861 ctatcttgtc cgccatttca ggaatcccgc gataggactc gtggacacga atatggtgaa
     1921 tatggggatg aaaaaagacg ggatatctta ccaggagcgg gcttatattt cgagagaggt
     1981 gaggatcaaa gatatggaag gccgtctctg gggagcgatg atgggcccgt tcggcggatg
     2041 ttacgctatc cggagggaag attactgcca ggtacccgcc aatttcctgg tggatgattt
     2101 ttatatcaat atgaccatct ttgagaaagg taaactggct gtcaatgact tgaattcgat
     2161 cgtttttgaa aatattccgg atgaactgaa agttgaattc cggagaaaag tgcgtatcgg
     2221 cacgggtaat tttcagaatc tgaggcgttt ctggaggctg gcattcacgc tgcgtttcgg
     2281 ttttgctttc atctcacaca aaattatccg ctggttcggt ccgtttttat tgctggcagc
     2341 gcttgttgcc aatatttacc tggctgtttt cagtagagtt tacctgttca cattggccgg
     2401 tcatctgctg gtttacttgc taccgttttt tgaccgcttg ctggggatga tgaagatgaa
     2461 tgcgggccct ttgcgcctgg caactcattt tttctcgatg aatgccgctt tgtttaccgg
     2521 ttttttccgg tcgctcggtt ctgtccgctc aggcgcctgg gagaggacca aacgcggctg
     2581 actttcaaaa gggctttgtt ccacacatcc atatccatca gcggagaatc ggtcgttgct
     2641 ttcaatgtca gaaagaatcc caggggcagc aaaaccactg acgaaagcca catccccgat
     2701 gccacctcca tagctccttt caaagccgat ttctcgccga tcatcgaaat aatataatac
     2761 aggatgaaga aaaggatcga aataacggtc ggcaggccca gccccccttt acggataata
     2821 gcgcccagcg gggcaccgat aaaaaacatc accaggcagg cgaaggaaag ggtgaatttc
     2881 cggtgccaga cgatcttatg tttgaccagt gattcgttgc tgacacggaa ggcatccgac
     2941 tgaaaatcca ggttcttttt gatgcccctg gccgaggtca acgcattgga aacgatccgg
     3001 atttgatcat cagtggaaaa accgctgaga aaatccccgg gcacaaccgt cactgaatcg
     3061 gtggagggca cgtatgccgt gtcgttattg agatatagct ggtaatttct cgggaagtta
     3121 cgggcgaggt tctcctttcg ttcgaccagc gaccggctta gggaatcgat gaaataattg
     3181 agctgaccga tgttcagcat ctggtaattg ttcttgaaaa gctcttcatt tgtccggttc
     3241 agctcgaatg ccgacaaatc gaatttacgt tgctggattt tgaaggaagt acgctgaaaa
     3301 ggctgttggt ttttggagtc tttctggtca actatgtcct ggtaattaaa gccgtcatac
     3361 agcgtaaaga tcaggtaccg gccatcgggt gtcgattcca tgatccccga atcagccacg
     3421 gtcacatcaa tatttcccct gttttcggtg tggttgtaaa tcattacttt atagatcctg
     3481 tttccgtctt tacccttttt tcccacacgg atcacataat cctccatatc attgtaaaag
     3541 attccttccc ggatattcac ggccagcttt ttcttccgga tatcgtaaag cagcgatttg
     3601 aacttgaggt tggccaccgg cagcacattg ttcgaaaaat aaaatgcaaa cgcggaaata
     3661 cataacgaaa ggatgatcag gggcttcatg atcgaccgca gggagatgcc ggccgacttc
     3721 atggcaacca gttcataatg ctcgccgagg ttgccgaatg tcatgagcga cgacagcagg
     3781 atggctaagg ggagggctag tggaacaaat gtaaatgaag cataaaagag cagttcggca
     3841 atgacatacc actccaggcc ctttccgaca aggtcgtcga tatacatcca catgaactgc
     3901 ataagaagaa tgaacagggc gataaaaaag gtcaccaata gcgggcctga ataggtttta
     3961 aggaccagtg cgttgatctt tttcatttta cactttagac tctaaacgca aaggtaagcg
     4021 tttaattgcc acttaaaaaa ttagagaatg gttaggaata ttccattttt atgttatttt
     4081 tgcacttcag gcgaaaccaa atgtttaatt aaataaagga aaaaaatgaa aaaactatta
     4141 ctcattgcca tggctttgat tatcggcgtc gtggcttatt cacaaaacaa ggttcagctt
     4201 agcaaggaga tgcgtgaaca gaccgtcacc ctgaccaggc aaaatgatat tgctgaagag
     4261 atcgttttcg gcaatttctc tgatcctacg gtcagcaaca gcaaagctgc cccgatcgaa
     4321 accctcatcg gcacgacaaa gtacgacctg atgacgaact caatgattca gaaccgtctt
     4381 tacctgcatg ccgacaatac catcggggct gtgtggacga tgggtctggg taccgagccc
     4441 gctttctcag acagaggcac cggctacaac tattatgacg gagcccagtg gggtgatgca
     4501 ccgaccgtaa ggattgaacc cgaaaggacc
//
LOCUS       DAOQXS010000221         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19577_length_4516_cov_3.674512, whole genome shotgun sequence.
ACCESSION   DAOQXS010000221 DAOQXS010000000
VERSION     DAOQXS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19577_length_4516_cov_3.674512"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PKM34_07055"
     CDS             complement(<1..909)
                     /locus_tag="PKM34_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ83384.1"
                     /translation="MNLTTDRRIVMTLDAGGTNFVFNAIRGGEEMFEPFRLKAKGATL
                     EEVLLKIIDGFNNVKSALDEKPSAISFCFPGPADYPRGIIGDLENLPTFRGGVALKDM
                     LEEKFGIPVFINNDGDLFAYGEAIGGILPEINARLEAAGNPRRYRNLFGVTFGTGFGG
                     GIVNNGQLFIGDNSAGGEVNRMRNRLYLSTSAEDSTAIRGVRRVYARETGIPFEESPN
                     PKEIQDIILGSFPGNKEAALKAWEEMAVVAGDTLANAITLIDGIIVIGGGLSGAHGVF
                     LQRLVDEMNASFTSLDGHEVPRLEITA"
     gene            complement(952..3789)
                     /gene="polA"
                     /locus_tag="PKM34_07060"
     CDS             complement(952..3789)
                     /gene="polA"
                     /locus_tag="PKM34_07060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNQ83385.1"
                     /translation="MEMNNRLFLLDAMALIYRAYFALNKNPRINSKGLNTSAILGFAN
                     TLYDLVKNEKPTHIGVAFDSFAPTLREAEYTEYKAQREKMPEDIALALPYIRRLLEGF
                     RIPILELPGYEADDIIGTLAHKADKKGFTTFMVTSDKDFGQLVSDRTFIYRPPRFGNK
                     AEILGIKEVCERYQIERPAQLIDILGLWGDASDNIPGIPNIGEKRAIELIKQYGSVEN
                     LIAHSGELQGKIRENVEAFAEQGLFSKKLATIILDVPLEFEEEKLILERPSFEILNEL
                     FNELEFRTFAKRVFTDLSVREQAGEEVTGLVAQAGGGKPQAGGDLFSELPATEKGTPS
                     LGTPSQGMASLDKTRVNYHLVTSKDQRQQLVDKLKHAEKFCFDTETTGIDPNNSELVG
                     ISFCFRPYEAYFVHLPANYQECLEIVSEFKPFLENENILKIGQNLKFDIAILKWYEVE
                     VRGKLFDTMLAHYLIEPDLKHGMDFLSETYLNYKTVHIDELIGKKGKDQGNMRDVQPE
                     ILSDYACEDADITYRLYEIFDPMLNEGGLRKLFEEIEIPLVPVLAAMEAEGVMLDTKA
                     LKDYSAHLGDEVHEVEEQIYDLAGQLFNIASPKQLGEILYEKLKITDKPPRTATKQYS
                     TSEDVLVKMLFRHPIIQLILDYRSLTKLKSTYVDVLPTLISPRDKRIHTSYNQAVAAT
                     GRLSSNNPNLQNIPIRTDRGKEIRKAFVPRNPDFILLSADYSQIELRIIAHMSGDKNM
                     IESFRQGTDIHAATAAKVYGIQVGEVTPEMRRKAKAVNFGIIYGQSAFGLAERLNITR
                     REAAEIIESYFAQYSGIREYMDRTIDFARKHGYVETILGRRRYLRDINSANAVVRGFA
                     ERNAINAPIQGSSADMIKIAMIRIHEDLKKLATRSRMILQVHDELVFDVHRSERELIR
                     PVVYEQMKNALPLDVPVEVEMDEGENWLEAH"
     gene            3994..>4516
                     /locus_tag="PKM34_07065"
     CDS             3994..>4516
                     /locus_tag="PKM34_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ83386.1"
                     /translation="MKNYLTLKQAVGWLRSIFLLLLVVAGTTVFGQEKSISGSVTDRS
                     TNESLPGASVLVKGTGAGTITDIDGKFALKVPPGGTSIVVSYVGYETQEITIGSQAVF
                     NVMLSPSKTSLQEVVVVGYGSTKVKDLTSSISTIKAEDINRTPASQPLQALQGKVPGL
                     QVVTSGGPGSMPTI"
BASE COUNT         1035 a         1194 c         1117 g         1170 t
ORIGIN      
        1 tgccgtgatc tcgaggcggg gtacttcatg cccgtcgagc gatgtaaagc tcgcattcat
       61 ctcatccacg agtcgttgca ggaatactcc gtgggcgccg gataatcctc cgccgatcac
      121 gataattccg tcgatcaggg tgatggcatt ggccagggta tcgccggcca caaccgccat
      181 ctcttcccag gctttcaggg ctgcttcttt attaccggga aaagacccga gaataatatc
      241 ctggatctct ttgggatttg gagattcctc aaacgggatg cctgtttcgc gggcatatac
      301 ccggcggaca ccccggatgg cggtgctgtc ttcagcgctg gtggagagat agagcctgtt
      361 gcgcatgcgg tttacttcac caccggccga attatcgccg atgaatagct gcccgttgtt
      421 gacaatgcct cccccgaaac cggttccgaa agtaacgccg aacaggttgc ggtatcgcct
      481 ggggttaccg gccgcctcca aacgggcatt gatctccggc aggatgcccc cgatggcttc
      541 accgtaggca aacagatcac cgtcgttgtt aatgaacacc gggatgccga atttctcttc
      601 cagcatgtct ttcagtgcga ctccacccct gaatgtaggc agattctcca gatcgccgat
      661 gatgccccgc ggatagtcgg ccggaccggg aaaacagaag ctgatggccg acggtttctc
      721 atccagtgcg ctttttacat tattaaaacc atcgattatt ttgagcagca cctcttccag
      781 tgtagctcct tttgctttca ggcggaaagg ttcaaacatc tcttccccgc cccgaatggc
      841 gttgaataca aagttggttc ccccggcatc cagggtcatg acaatgcgtc tgtcggtggt
      901 caggttcata atcttggaat ttggaatgat caaaaataga aaaatattat ttcaatgcgc
      961 ctccagccag ttttcccctt catccatctc aacctccacc ggtacatcca gcggcagtgc
     1021 atttttcatc tgctcataca caaccggccg gatcagttct ctttccgatc tgtgtacgtc
     1081 gaaaacgagt tcgtcatgga cctgcaggat catccggctg cgagttgcaa gctttttaag
     1141 gtcttcatgg atgcggatca tggcgatctt gatcatatcg gccgatgaac cctggatcgg
     1201 ggcattgatg gcgtttcttt cagcaaaccc gcgcacaacg gcattggccg aattgatatc
     1261 gcgcagatac cgccttcttc ccaggatggt ctccacatac ccgtgcttcc gggcaaaatc
     1321 gatggtgcgg tccatatatt cccgaatccc cgaatactgt gcgaagtagg attcgatgat
     1381 ctctgccgct tcgcggcggg taatgttcag ccgttcggcc agtccgaacg ccgactgccc
     1441 gtaaatgata ccgaaattga ccgccttggc tttccggcgc atctccgggg tgacttcgcc
     1501 gacctggata ccgtagactt tggcggctgt ggcggcgtga atgtcggttc cctgccggaa
     1561 agattcgatc atgtttttat ctccgctcat atgtgcaatg atccgcaact caatctggga
     1621 gtaatcggcc gataaaagga tgaaatcggg atttcggggt acaaaagctt ttcggatctc
     1681 ttttccccgg tcggtccgga tcgggatatt ctgcaggttc ggattgttgg aactgagccg
     1741 gcctgttgca gccaccgcct ggttgtaaga ggtgtggatc cgtttgtcgc gcgggctgat
     1801 cagggtgggc aacacatcga cgtaggtcga tttaagcttg gtcagtgaac ggtagtccag
     1861 gatcagctgg atgatcggat ggcgaaacag catcttgacc agcacatcct cgctggtcga
     1921 atattgtttg gtagccgtgc gcggcggctt atcggtgatt ttaagctttt cataaagaat
     1981 ttcgccgagt tgtttgggcg atgcgatatt aaagagttgc ccggccaggt cgtagatttg
     2041 ctcctccacc tcatggacct catcgcccag gtgtgcggaa taatctttca aagccttcgt
     2101 gtcgagcatt acgccttccg cctccatggc ggccaggaca ggcaccagcg gaatttctat
     2161 ctcttcgaaa agctttctga ggccgccttc attcagcatc ggatcgaaga tttcatatag
     2221 gcggtaggtg atgtcggcat cttcgcaggc ataatcggaa aggatttccg gttgcacatc
     2281 ccgcatattc ccctggtctt tgcctttctt gccgatcagt tcatcgatat ggacggtctt
     2341 gtaattaagg tatgtttcgg aaagaaaatc cattccgtgt ttcagatccg gttcgatcag
     2401 gtagtgggcc agcatcgtgt cgaacaattt gccgcgtacc tccacctcat accatttgag
     2461 gatagcgata tcgaatttga ggttttgtcc gatcttgaga atgttttcgt tttccaggaa
     2521 gggtttgaac tcgctgacaa tttcgaggca ttcctggtaa ttggccggga ggtgcacaaa
     2581 ataagcttca taaggccgga agcaaaagga gatgccaacg agttccgagt tgttcggatc
     2641 gatgccggtg gtttcggtat cgaagcagaa cttttcggca tgtttgagtt tgtccaccag
     2701 ttgctgccgc tggtctttgg aagtgacgag atggtaattg acccgggtct tgtccaggga
     2761 cgccatccct tgggatggcg tcccaaggga tggcgtccct ttctcggtgg cgggcaattc
     2821 gctgaaaaga tctcccccag cctgcggttt ccccccgccg gcctgggcca ccaacccggt
     2881 tacctcttct ccagcctgct cccttaccga aaggtcagtg aataccctct ttgcgaaagt
     2941 cctgaattcc agctcattga acaattcgtt gaggatctcg aaagagggtc tctccagtat
     3001 cagcttctcc tcttcaaatt ccaaaggcac atccagaatt atggtggcca gcttcttcga
     3061 gaaaagcccc tgctctgcaa aggcttcgac attttccctg atctttccct gcaactcgcc
     3121 ggaatgggca atcaggttct ccaccgatcc gtattgcttt atcagctcta tggccctctt
     3181 ttccccgatg ttcggtatcc ccgggatatt atcggacgca tcgccccata atcctaaaat
     3241 atcaatcaat tgcgccggcc tttcaatctg gtatctttca cacacttcct ttatccctaa
     3301 aatctccgct ttgttgccaa acctgggcgg acggtaaata aaggtcctgt cgctgacaag
     3361 ctgcccaaaa tccttgtcgg acgtcaccat gaacgtcgta aatccctttt tatctgcttt
     3421 gtgtgcgagc gttcctataa tatcatccgc ctcataaccg ggcagctcga ggatggggat
     3481 acggaatccc tccagcaaac gacggatata gggcaacgcc agggcaatat cttccggcat
     3541 cttctcccgc tgggctttgt attcggtgta ctctgcctcg cggagcgtcg gcgcaaacga
     3601 atcaaaagcc accccgatat gggtcggctt ttcattcttg acaagatcat ataaagtatt
     3661 cgcaaaacca agaatagcgg aggtattcaa tccttttgaa ttgatcctgg gatttttatt
     3721 cagggcgaaa taagccctgt aaatcagggc catagcatct aataaaaaga ggcggttatt
     3781 catttccagt gagtattgag caaagataat caaaggaaat tcaaaaccgc atcctgataa
     3841 tcccgtatgg acccccaaac acgattaaac ataatatttt cacattttcc gaaaacacca
     3901 ttattattat atttgtaaga aggattgatt ttacccggta tgacttccac aaacccgaaa
     3961 atatttctta actaatacca aaatcaatat gttatgaaga attaccttac acttaaacag
     4021 gctgtcggct ggttaaggag catttttctg ctcttgctgg tcgttgcagg aaccactgtg
     4081 ttcggccagg agaagagcat cagcggctcg gttaccgacc ggtcaacgaa cgaatccctg
     4141 cccggagcat cggtattggt aaaaggtacc ggagccggta cgattaccga tatcgacggt
     4201 aaattcgcat tgaaggtgcc tcccggcgga acatccattg tcgttagcta tgtgggctac
     4261 gaaacccagg agataacgat cggaagccag gccgttttca atgtcatgct atccccttcg
     4321 aaaacctctt tgcaggaagt agtggtcgta ggatacggaa gcacaaaagt gaaagacctg
     4381 acctcttcca tcagtaccat caaagcggaa gatattaacc ggactcccgc ttctcaaccg
     4441 ctgcaggccc tgcagggaaa ggtccccggt ctgcaggtcg tcacttccgg aggccccggc
     4501 agtatgccca ctatcc
//
LOCUS       DAOQXS010000222         4512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19597_length_4512_cov_4.332511, whole genome shotgun sequence.
ACCESSION   DAOQXS010000222 DAOQXS010000000
VERSION     DAOQXS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19597_length_4512_cov_4.332511"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..529)
                     /locus_tag="PKM34_07070"
     CDS             complement(116..529)
                     /locus_tag="PKM34_07070"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNQ83387.1"
                     /translation="MLALPFIVAGLTLNILYPEIFSVCGPTLGLIIASVIILIPGIII
                     WIWSVVLILTKIPRRELITTGPYALVKHPLYTGVALLVLPWVGFLCNTWLGALIGIVV
                     YIGSRLYSPEEEKILAKIFGQAWDDYCNKVLLPWI"
     gene            complement(796..3768)
                     /locus_tag="PKM34_07075"
     CDS             complement(796..3768)
                     /locus_tag="PKM34_07075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNQ83388.1"
                     /translation="MKNPALPHLLTTLITLITFITLITFTTLTTHAQWSTDPAINNAI
                     IALSGEEAIPKIATCSNGDTYIASFSNNGNYNVRMQRLNAQGIPQWADDGILISDNPQ
                     DSWLTDWDICADNANHAILVFPDIRNGGANVVAYRISPTGTFAWGDDGIVLSTGDANY
                     SPKVTATAAGNAIVAWMQDDVIRVQKISPTGSLPWGSGGLVLQQTGITFSWPQLLPVG
                     TDEFIMKYYQDSGTFPAITRHIFAQRYNAGGTGVWSSPTVISNAGGIAAWTQVLPFIN
                     DGSDGFYIAWHDDRDNNMLSSTWVNHVNSSGAVLFTANGVEASTLGGRNHFYPQLALP
                     PGSSNVFVFWNEMDGDQNNRGIYGQKITSTGSKLWATTGMIFIEISPVDVYPEAARHS
                     PDDVVVFYSEQVSGLDYTLKAFRIDADGGYIWTPNHKTLCSVVSEKVHVETNDFADNQ
                     WIMAWEDDRAGQKDIYAQNIQLDGSLGPYDPNEGTIEGVVTLGGGAGSVTQVTVTAGE
                     YSTNPDNAGFYSMVVEEGTYDVIGMLQGYYPDTVFSVMVITGETTEDVDLTLQALPTG
                     FISGNVSLSEANADLTEVTVTAGLHTVNPDANGDYIMEIEIGTYEVTASLDGYMPETE
                     TGVVVVEGQTTTGVDFILDPVPTTGFITGIVLLAGGDGEVTEVNVTAGNVTVNPGEDG
                     IYLLEIVAGTYEVTASLPGYLTGVVPNVVVLVEQTTPDIDFYLYEAPDIGYIEGWVTL
                     NGPGDVTQAVVTAGNQSDNPNAQGYYFLGLPAGTYTVTADHPYTLPETLEGIEVIAGQ
                     NSENVNFGLDVLRCNLSVKAVDQYGSPLSDVEVDIIGPGDIYTGIIENDSVIFEDVPY
                     GLYAGTAWLVMSPDTARTNDEINDSDQDMEFVFILGGMNDESQTRVRSFSISPNPFSE
                     EVRLSFEVENPVEVILKVYSSTGRLIKILAEGRFDRGRHEVQWNGDEDGGNRLPAGIY
                     TVVFQAGGEREAKVLILR"
     gene            complement(4001..4441)
                     /locus_tag="PKM34_07080"
     CDS             complement(4001..4441)
                     /locus_tag="PKM34_07080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNQ83389.1"
                     /translation="MKKIMIGLISAMVLYFVFFLGNYFSRLWFDFAGPGIENVYKFKG
                     DAAGIRIAILMLLIIGPGEELFWRGYLQRKFSKKYGKWTGLIIALILYTGVHIFTGNF
                     MLIMAAFICGLFWAWMYLKYESMLINVISHTAWDIVVFLLLPFH"
BASE COUNT         1179 a         1130 c         1123 g         1080 t
ORIGIN      
        1 gatgtgcagg tcgggcccga cggatcattg ttcgtttcgg atgatttagc cggatgtgtt
       61 taccggattt ttaagtgata ttcgatttac gatttacgat tttcgatatg gtctcttata
      121 tccaggggag gagaacttta ttgcaataat cgtcccaggc ctggccgaag atcttggcca
      181 ggatcttttc ttcttccggc gaataaagcc tcgatccgat gtaaactaca atgccgatca
      241 gggccccgag ccatgtgttg cacaggaagc cgacccaggg caggacgagt agcgccaccc
      301 cggtgtagag cggatgcttg accagagcat aaggcccggt ggtgatcagt tccctgcggg
      361 gaatcttcgt caggatcaaa accactgacc atatccagat gataatgccc ggtatgagta
      421 tgatgaccga agcgatgata agaccaagtg tcggtccgca gacgctaaag atttcggggt
      481 agaggatgtt cagggttaaa ccggcaacga tgaacggcag ggccagcaat ccgatttttc
      541 ttccgttccc gacaaggatt tacagcatca taatgtacag ataataagat tgttagaaga
      601 agattgcctt ttagttctct ttgattttca tcaaccttgt ttaagcgcaa attccttcaa
      661 gttgataaaa cattaggtaa aggtataaaa cagaaggcat taaattgaga tggagtctcg
      721 tttaaatgag actccatctc aaaaaaaggg acgccatccc ttcgggacga ggtcccgagg
      781 gacgccatcc ctttcctatc tcaggatcaa aaccttagcc tccctttcac ctccagcctg
      841 gaaaacaacc gtgtaaatcc ctgccggcag acggttgcct ccgtcctcat caccgttcca
      901 ctgaacctcg tgcctgccac gatcaaacct gccttcggca aggattttta tcaatcgccc
      961 tgtagaagaa taaaccttca gaatcacctc gacaggattc tcaacttcga acgacagcct
     1021 gacctcttca gagaagggat tcggtgaaat gctgaatgac ctgacccggg tttggctttc
     1081 atcattcatc ccgccaagga taaaaacaaa ctccatatcc tggtcgctgt cgttgatctc
     1141 gtcgttggta cgggccgtat cggggctcat caccagccat gcagtaccgg catacaatcc
     1201 gtaaggcaca tcttcaaaaa taaccgaatc attctcaata attcccgtat aaatatcccc
     1261 aggtccaata atatccactt caacatccga taaaggagaa ccgtactggt cgacagcttt
     1321 caccgacaga ttacaacgca ggacatcgag cccgaagttg acattttcac tattctgccc
     1381 ggcgatcacc tctattcctt ccagggtttc gggtaaagta taaggatgat cggcagtcac
     1441 ggtgtatgtc cctgccggaa gtcccaggaa gtaataaccc tgggcgttcg gattgtcgga
     1501 ctggttgccg gccgtcacaa ccgcctgggt cacatcgcca gggccgttca gcgttaccca
     1561 tccttcgata taaccgatat cgggcgcttc atagagatag aagtcaatat caggagtcgt
     1621 ttgttccacc agtaccacca cattgggtac taccccggtc aggtagccgg gcagactggc
     1681 cgtcacttca tacgttccgg ccacaatttc gagcaggtaa atcccatctt caccgggatt
     1741 aacggtcaca ttgcctgccg taacattgac ttcggtcacc tctccgtcac ctcctgccag
     1801 gaggacaata cccgtgataa atcctgtagt gggcacagga tcgaggataa agtcgacacc
     1861 ggtcgttgtc tgaccttcca cgaccacaac accggtctcg gtttccggca tatatccatc
     1921 cagcgaagcc gtcacctcgt aggtgccgat ctctatctcc atgatatagt cgccgtttgc
     1981 atcgggattc accgtatgca atcccgccgt aactgtgacc tccgtcagat cggcattggc
     2041 ttcggaaagg gagacattgc cgctgatgaa tcctgtcggc agggcctgga gcgtaagatc
     2101 aacatcttct gttgtttcac cggtgatcac cattacgctg aataccgtat cgggataata
     2161 accctgcagc ataccgatca catcgtaagt gccttcttcc accaccatcg aataaaaacc
     2221 cgcattatcg ggattcgtgc tgtattcacc agccgtaacg gttacctggg tcacactgcc
     2281 tgcacctccg cccagggtca ctactccttc aatggtccct tcattgggat cgtaaggccc
     2341 gagcgatccg tcgagctgga tgttctgtgc ataaatgtct ttctgtcctg cccggtcgtc
     2401 ttcccaggcc atgatccact ggttatcggc aaaatcattg gtttccacat gaactttttc
     2461 ggaaacgacc gaacaaagtg ttttatggtt gggcgtccag atatatccgc catctgcatc
     2521 gatccggaaa gctttaaggg tataatccaa tccggacacc tgttcggagt agaaaacaac
     2581 cacatcgtcc ggggaatggc gtgctgcttc cggatagaca tcgaccggag aaatttcgat
     2641 gaagatcatc ccggtcgtcg cccatagttt tgaacctgta gaagtgatct tctgcccgta
     2701 aatcccgcgg ttgttctggt cgccgtccat ttcattccag aaaacgaaca cattggaaga
     2761 accgggaggc agggcaagct ggggataaaa atggttgcgc ccgccgaggg tggaggcttc
     2821 caccccgtta gccgtgaaca gcacagcgcc ggaactattg acatggttga cccaggtact
     2881 ggagagcata ttgttgtcac ggtcgtcgtg ccatgcaatg tagaatccgt cggagccgtc
     2941 gttgatgaaa ggcagtacct gggtccaggc ggcgatacct cccgcattgg atatcacggt
     3001 gggggatgac cagacgccgg taccgcctgc gttgtagcgc tgtgcaaaga tatgccgggt
     3061 gatggccggg aacgtacccg aatcctggta atatttcata atgaattcat cggtgcccac
     3121 cggcaggagc tgtggccagg agaaagtgat cccggtctgt tgaaggacca gtcctcccga
     3181 tccccaggga agggacccgg tcgggctgat cttctgcacc cggatcacat cgtcctgcat
     3241 ccaggccacg atggcatttc cggctgcggt agccgtaacc ttcggcgagt aattggcatc
     3301 gccggtagag aggacgatac cgtcgtcgcc ccaggcgaaa gtaccggttg gcgaaatccg
     3361 gtaggctacg acattggcac cgccgttacg gatatccgga aaaacaagaa tagcgtggtt
     3421 ggcgttatca gcgcagatat cccagtcggt gagccagctg tcctgcgggt tgtcgctgat
     3481 gaggatgccg tcatcggccc actgtggtat accctgggcg ttgagccgtt gcatccgtac
     3541 gttgtaattt ccgttgttgg aaaaggaagc aatataggta tcgccgttgg aacaggtcgc
     3601 gatcttcgga atggcttctt cccctgacaa agcgataatg gcgttattga tggcggggtc
     3661 ggtgctccac tgggcatgtg tggtaagggt ggtaaaggtt ataagggtta taaaggttat
     3721 aagggttata agggtggtaa ggagatgggg taatgctgga tttttcatgg gttaaggtat
     3781 tgaattgtct gtaaaaatac atgattcaac actgttaatg ttcaattatc ctgaaaaaat
     3841 cacataagaa acccataaaa ataccatgga acaaaagaca aagacaatag aaatagaaat
     3901 agaacaatag aacaatagaa caatagaaca acagaacaat agaacaacag aacaatagaa
     3961 caatagaaca attgaacact ggaacaacag aaccctgaac ctagtggaac ggcagcaaga
     4021 ggaacaccac gatatcccag gcggtatggc tgatcacgtt gataagcatc gactcatatt
     4081 tcaggtacat ccaggcccag aagaggccgc agatgaaggc tgccatgatc agcatgaaat
     4141 tgccggtgaa gatatggacg ccggtataaa ggataagggc gatgatcaga ccggtccatt
     4201 taccgtattt ttttgagaat tttctttgaa gatatcccct ccagaaaagt tcttcgccgg
     4261 gaccgatgat cagcagcatc aggatggcaa tccggattcc ggcggcatct cccttgaatt
     4321 tatatacgtt ttcgattccc gggccggcga aatcgaacca gaggcgcgaa aaataatttc
     4381 cgaggaaaaa tacgaagtac aggaccatgg cggagataag cccgatcatt attttcttca
     4441 aaatgctctt cgtgaaatct tatatgtttg tttttttatt caaacaaata ttgttatgct
     4501 tgcaacaaag gg
//
LOCUS       DAOQXS010000223         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19607_length_4509_cov_3.342389, whole genome shotgun sequence.
ACCESSION   DAOQXS010000223 DAOQXS010000000
VERSION     DAOQXS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19607_length_4509_cov_3.342389"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKM34_07085"
     CDS             <1..643
                     /locus_tag="PKM34_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83390.1"
                     /translation="HGFGSPVGKLNVSTVPLEILSDGDLAALGIITGEEASLEFESGL
                     MVTGRLERIVRNRDKIVVMSFSGCTVRFRDKVLFDPSWGLYDMAVGEKIVSAYAGPAD
                     PFAYRLEYPVPKEKTHKIIHSDRAARLHGLYQAVRDHREGKQMIPDPVQLFNELKNDY
                     PEEWLLPLEILEIIHGRSGYQLHEEIKSYLNQKGRENEDFGRLIKRGIEEIDS"
     gene            complement(640..1527)
                     /locus_tag="PKM34_07090"
     CDS             complement(640..1527)
                     /locus_tag="PKM34_07090"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ83391.1"
                     /translation="MIHLNPAEIPHTKLHQYLLSGIAPRPIAFASTVDASGRPNLSPF
                     SFFNVFGVNPTTLIFSPSRRSRDGSVKDTLENLKAVPEVVINVVTHDMVNQASLASSE
                     YPKGVDEFVKSGFTKLPSEIIKPFRVKESPMQMECRVREIIETGTGGGAANLVICEIL
                     LIHISEDILGEDGMIDQHKIRLVGRLGKDFYVKAFGDALIEVEKPARNIGIGIDALPE
                     FIRHSAHLTGNDLGKLGNLERMPSLEEIEEMRQSQDITEILETSCDVYGEVLRLAKAW
                     LDSGHPFKALKILMLLEKI"
     gene            complement(1600..2841)
                     /gene="fahA"
                     /locus_tag="PKM34_07095"
     CDS             complement(1600..2841)
                     /gene="fahA"
                     /locus_tag="PKM34_07095"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNQ83392.1"
                     /translation="MYTSWINIPPENDFSIFNIPFGIISYKGYSPRPATRIGDTVVDL
                     AALAGYGFFDGLGISDLSVFNRPALNDFIALGKPVTRAVRDRLISLFRSDNPELRDHS
                     EARRTSLLNAVDVTMHLPVKVGNYTDFYASEQHAYNVGVMFRDPKNALLPNWKHIPVG
                     YHGRASSIVVSGASIHRPMGQTKADDQEFPAFGPSKQVDFELEMGFVIGKSSALGDRV
                     KISDAEDYIFGLVLFNDLSARDIQKWEYVPLGPFLSKNFGSVISPWIVTTEALEPFRV
                     PGPVQVPEVLPYLKYQGNHHFNIKLEVMLQGKLLTVTNSKHLYWNFAQMLAHHTVNGC
                     NVEVGDIYGSGTISGPEQGSYGSMLEIAWKGTKPVVMPDGTSRSFIHDHDTIVIKGFG
                     EKQGIRVGFGECKVTILPAKK"
     gene            complement(2902..3996)
                     /gene="hppD"
                     /locus_tag="PKM34_07100"
     CDS             complement(2902..3996)
                     /gene="hppD"
                     /locus_tag="PKM34_07100"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNQ83393.1"
                     /translation="MSTDFLPLNGTDYVEYYVGNAKQAAYYYMTAFGFQPLAYSGLET
                     GNKEKTSYVLVQDKIRFVFTTSLVPDSEIGEHVKKHGDGVKVVALWVDDATSAFNETV
                     KRGAKPYLDPVKEQDAMGHVVRSGIHTYGETVHIFVERKDYRGAFLPGFSKWEPAYRT
                     EPIGLKYVDHMVGNLGWGEMNKWAKFYEDVMGFKQLISFDDKDISTEYTALMSKVMSN
                     SNGYIKFPLNEPAEGKKKSQIEEYIDFYGGAGVQHVAMATDNIIHTVSELKKRGVEFL
                     YVPGTYYEDVLDRVGHIDEDLKPLKDLNILIDRDDEGYLLQIFTKPVEDRPTLFYEII
                     QRKGAKSFGKGNFKALFESIEREQARRGTL"
     gene            complement(4050..>4509)
                     /locus_tag="PKM34_07105"
     CDS             complement(4050..>4509)
                     /locus_tag="PKM34_07105"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNQ83394.1"
                     /translation="HPFDVIGWDGYHFPYALSIHDFEPITGRIHQPPPVHQTFEANNF
                     VMCAFVPRLYDYHPEAIPVPYNHSNVDSDEVLYYVDGEFMSRKHVEKGQITLHPIGIP
                     HGPHPGTVEKSLGQKETRELAVMVDTFRPLKITKQAMEIEDSEYFRSWAE"
BASE COUNT         1138 a         1078 c         1080 g         1213 t
ORIGIN      
        1 tcacggcttc ggatcgcctg tcggaaagct gaatgtttcg acagtacctc tggagattct
       61 ttcagatggg gatcttgccg ctcttggcat aataacagga gaagaagcaa gcctggaatt
      121 cgaatcaggc ctgatggtta ccggaagact ggaacggatt gtcagaaata gggataaaat
      181 agttgtgatg agtttttccg gctgtacggt gagatttcgt gataaagtcc tttttgatcc
      241 ttcctggggt ttgtatgata tggctgtcgg ggaaaaaatc gtttccgcct atgccggccc
      301 ggcagatcca tttgcttacc gtctcgaata tcctgttccg aaggaaaaaa cacataaaat
      361 tattcacagc gaccgggcgg cccgacttca cggattatac caggctgtga gggaccacag
      421 ggaaggaaaa caaatgatac ctgacccggt tcagcttttt aatgaactta aaaacgatta
      481 tccggaagaa tggcttctgc ctttggaaat attggaaatt atccacggca ggtcaggtta
      541 tcaattacat gaggaaatca aatcctatct gaaccaaaag ggaagagaga atgaagattt
      601 tggaagactg ataaagagag gtattgagga aatcgattct taaatttttt ccagcaacat
      661 caatatttta agcgctttaa atgggtgtcc tgaatcgagc caggctttag ccagccgcaa
      721 aacttcgccg tatacatcgc acgaagtttc cagaatttca gttatatcct ggctttgtcg
      781 catttcttct atctcttcca gagatggcat cctctccagg tttcccagtt tacccaggtc
      841 atttcccgta agatgggcgc tatggcggat aaattcaggc agggcgtcga tcccgatccc
      901 gatattacgg gccggttttt ccacttcaat gagggcatca ccaaaagctt tcacataaaa
      961 gtccttgcct aacctaccca ccagcctgat cttgtgctgg tcgatcatcc catcttctcc
     1021 caaaatatct tcgctgatat ggataagaag gatctcgcaa ataacgagat ttgctgcccc
     1081 gccccctgtt ccggtttcaa tgatctccct cacacggcat tccatctgca ttggcgactc
     1141 cttcacgcgg aatggcttta tgatttcgga tggtaatttc gtgaaaccgg atttgacaaa
     1201 ttcatcgact cctttgggat actcggaact cgccaggctg gcctggttga ccatatcgtg
     1261 ggtaaccaca ttaattacga cttccggaac ggctttcaga ttttccaggg tatctttcac
     1321 agaaccgtcc cgacttcgtc gggatggtga aaaaataagc gttgtgggat ttacgccgaa
     1381 aacattgaag aaactgaaag gcgacaggtt cgggcggcct gatgcatcca cagtactggc
     1441 aaaggcaatc ggcctggggg cgatacctga cagaaggtat tggtgaagtt tcgtatgggg
     1501 aatttcggcc gggttgagat ggatcatggg aacgtgatgt gaatttaaag ttcaaagtta
     1561 taattactaa ggattgggaa atgggaagta atcacttgtt tatttttttg cagggaggat
     1621 agtgacttta cactcgccga aacctacccg tattccctgc ttttcaccaa accctttgat
     1681 cactatcgta tcgtggtcgt ggatgaagct tcgggatgtg ccatcaggca tcacaaccgg
     1741 tttggtccct ttccaggcaa tttccagcat cgaaccgtat gagccttgct ctggcccgct
     1801 gatcgtgcct gatccgtata tatcgccaac ttcaacatta cagccgttca ccgtatgatg
     1861 cgccagcatc tgggcaaaat tccagtatag gtgttttgaa ttggtaactg tcagaagttt
     1921 gccctgaagc ataacttcca gtttaatgtt aaaatgatgg tttccctggt attttaaata
     1981 gggcaaaact tccggaacct gaacagggcc gggaacgcga aaaggctcca gggcttccgt
     2041 ggtcacgatc catggtgaga tcacgctgcc gaaattctta ctcaggaaag gccccagcgg
     2101 aacgtattcc catttctgaa tatcacgggc cgacaagtcg ttgaacaata ccaggccgaa
     2161 tatataatct tcagcatcag aaatcttaac acgatcacct aaagctgaac ttttaccgat
     2221 aacgaatccc atttcgagtt cgaagtcgac ctgctttgaa ggcccgaagg cggggaactc
     2281 ctggtcgtcg gctttggtct ggcccatcgg acggtgaata gatgcaccgc tgaccacgat
     2341 cgaggaggcc cggccgtggt atcctaccgg aatatgcttc cagttgggca gaagggcatt
     2401 tttcggatcg cggaacatca cgccgacatt gtaagcatgc tgttcgctgg catagaaatc
     2461 ggtataattt cccactttca ccggcaggtg catcgtcaca tcaacggcgt tcagcaatga
     2521 cgttcttcgg gcttcggaat ggtctctcag ttccggatta tcagaccgga agagcgaaat
     2581 caaccggtca cgaacggccc gggtcaccgg tttgccaaga gcaataaaat cgttcaatgc
     2641 cgggcgattg aaaacggaga gatcgctgat ccccaatccg tcgaaaaagc cgtatccggc
     2701 cagcgccgcg aggtccacga ctgtatcccc tatccgggtt gccggccgtg gagaatatcc
     2761 tttatagctt attattccaa aagggatatt gaatatggag aaatcgtttt caggcggaat
     2821 atttatccag gaagtataca tctctatcta tttgccacta aagcactaaa acaccaaatt
     2881 acactaaaag tcaagtctgt cttaaagggt tccgcggcgg gcctgttccc tttcgatgga
     2941 ttcgaacaaa gctttgaaat ttcctttacc gaatgatttg gcccctttgc gctggataat
     3001 ctcatagaac aacgtcgggc ggtcttcaac cggtttggta aagatctgaa gcaggtaacc
     3061 ttcgtcgtcc ctgtcgatca ggatattcag gtctttcaaa ggtttcaggt cttcatcgat
     3121 atgtccgaca cggtcgagaa catcttcata atatgttccg gggacatata aaaattccac
     3181 tcccctcttt ttcaattcgc tgaccgtatg tatgatgtta tcggttgcca tggcaacatg
     3241 ctgcactccc gctcctccat agaaatcgat atattcttcg atctgcgact ttttctttcc
     3301 ttctgccggt tcattgagtg gaaatttgat ataaccgttg ctgttcgaca tcaccttcga
     3361 catcagtgcc gtatattcgg tagagatatc cttatcgtcg aatgagataa gctgtttaaa
     3421 ccccatcaca tcctcgtaga atttcgccca tttgttcatt tcgccccagc ccagattgcc
     3481 gaccatatga tccacatact tcaggccgat gggttcggtc cggtatgccg gttcccattt
     3541 tgagaatccc gggaggaaag cgccccggta atcctttcgc tcgacgaaga tatgaaccgt
     3601 ttctccatag gtatggatgc ctgatcgcac aacatgaccc attgcatctt gctcctttac
     3661 cggatcaaga taaggttttg caccccgttt caccgtttcg ttgaacgcac tggttgcgtc
     3721 atctacccac aaggcaacca ctttgacccc atcgccatgc tttttaacat gttcgccgat
     3781 ttccgaatcg gggaccaggg aggttgtaaa aacaaaccgg atcttatcct gtaccaggac
     3841 ataggatgtt ttttccttgt taccggtttc aaggccggaa taggccagcg gctggaagcc
     3901 gaaagcggtc atataataat aggcggcctg tttggcattt ccgacatagt attccacgta
     3961 gtcggtgccg ttgagaggga gaaagtctgt gctcattgaa agttgaaatt agaaatttga
     4021 aagtttgata atttgataat ttgataattt cactctgccc agctccggaa gtattcgctg
     4081 tcttcaattt ccatcgcctg ctttgtgatt ttcagggggc ggaacgtatc gaccatgaca
     4141 gccagttcgc gcgtctcttt ttgtccgagg cttttttcga cagttcccgg gtgcgggccg
     4201 tgcgggatgc cgatcgggtg cagggtgatc tgccccttct ccacgtgctt gcggctcatg
     4261 aattccccgt cgacataata gagcacctcg tcggaatcga cattgctgtg attataaggc
     4321 acgggaatag cttcgggatg gtagtcataa agccttggca cgaaagcgca catgacgaaa
     4381 ttgttggctt caaaggtctg gtgaaccggc ggcggctggt ggatccgtcc ggttatgggt
     4441 tcaaaatcgt ggatagacaa agcatatgga aaatgatatc catcccatcc gatgacatca
     4501 aaaggatgg
//
LOCUS       DAOQXS010000224         4503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19638_length_4503_cov_3.500450, whole genome shotgun sequence.
ACCESSION   DAOQXS010000224 DAOQXS010000000
VERSION     DAOQXS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19638_length_4503_cov_3.500450"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKM34_07110"
     CDS             complement(<1..883)
                     /locus_tag="PKM34_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83395.1"
                     /translation="MSDIKTMNRWLVVIGAIMIQLALGAIYAWSVYTKTLENSGWSKQ
                     ETQMVFSAGLALFAIVMVVAGRFLQHPKVGPRRLAMASGLVLGLGYVLAGIFGAENFT
                     TTFIFVGIIGGAGIGIGYVVPIAVGIKWFPDKKGLITGLAVAGFGFGATLWMTLAGKL
                     GPLGGGELLKSLGVSGTFIAFGIAYFVLVEIGSIWMKFPPDGFKPAGWNPPQATGSSP
                     VAGSVTLTSAEMLRTPQFYFILLTFAFGASAGLMSIGLMKLWPMEALEANGIAPETAS
                     AMATLAMAVFLPFSMASA"
     gene            complement(1031..2944)
                     /gene="acs"
                     /locus_tag="PKM34_07115"
     CDS             complement(1031..2944)
                     /gene="acs"
                     /locus_tag="PKM34_07115"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNQ83396.1"
                     /translation="MMKKISSFVDYFQQYHESVTNPEGYWAKIADTFQWHKKWDKVLD
                     WNFTEPKIKWFDGGKLNITENIFERNLPARKDQVALIWEPNDPKEPEKRITYGELFEK
                     VCIFANALKRQGIQKGDRVAIYLPMVPELPIAMLACARIGAIHSIVFAGFSANSLADR
                     INDSACKMVITADGLYRGSKNVGLKSIVDEALQMCTSVEHVVVLKRTGDPVQMKEGRD
                     IWWDAFIEGVSKENKAEVMEAEDTLFILYTSGSTGKPKGVLHTTAGYMVFAQFTFLNV
                     FQYNEGDIYWCTADIGWVTGHTYIVYGPLLSGATSVMFEGVPTYPDPGRFWETVDKYQ
                     VNQFYTAPTAIRALIAQGDEWVRSKNLSSLKVLGSVGEPINEEAWRWYHDVVGKGKCP
                     IVDTWWQTETGGILITPLAGITPTKPSFATLPLPGVQPVLVDNEGNVMEGNNVQGNLC
                     IKFPWPGMIRTTYGDHERCRINYFSTYRGLYFTGDGAKRDEEGYYRILGRVDDVINVS
                     GHRIGTAEVEDAINQHPKVVESAVVGYPHEIKGSGIYAYVTCDALSEEEVQTIDKEIC
                     DIVSRIIGPIAKPDKIQIVSGLPKTRSGKIMRRILRKIGEGDATSLGDTSTLLDPGVV
                     EEIIRGAKVEVKR"
     gene            complement(3009..4250)
                     /locus_tag="PKM34_07120"
     CDS             complement(3009..4250)
                     /locus_tag="PKM34_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83397.1"
                     /translation="MKKPLLLLFLAYFPVMLFSQDDKTFGIKFTGFVKTDVIWDSRQT
                     VDLREGHFLLLPKPVLNDLEGNDINAKPSFNFLSIQTRLKGDITGPDALGAKTSGSIE
                     GEFFGMADGDINGFRLRHAYVKLNWKTTELLVGQMWHPMFITSSFPEVVSFNTGAPFL
                     VFSRNPQIRLTKDIKSFRLILTALSQVDFKSNGPDGASTKYIRNSSIPSFNLNFEYSY
                     KNKETGTEILAGAAGNFKRLLPRMITDSGYQTNEGINSLSGMVYLKLGFQKVIFKMAG
                     LYGQNMFDYTMIGGYVVDDYLDKDKGIVSYRNITTASVWAEVMTTGKKMQGGLFLAYS
                     QNMGAGDIVIGPYYSRGANIYDLYRISPRFVYNAGKFRIAPEIEYTVARYGMTNNLDG
                     KVMDPEPVGNFRLLVGVFYFF"
BASE COUNT         1112 a         1140 c         1086 g         1165 t
ORIGIN      
        1 cggccgaggc cattgaaaag ggcaaaaata cggccatggc cagtgttgcc atagcgctgg
       61 ctgtttcagg tgcgattcca tttgcctcca gtgcttccat tggccatagc ttcatcaggc
      121 cgatgctcat caggccggcg ctggctccaa aagcaaaggt cagcaggatg aaataaaact
      181 gaggtgttcg cagcatttct gctgaggtca gggtgaccga accggcgaca ggggacgatc
      241 ctgtggcttg cggagggttc cacccggcag gcttgaatcc gtccggagga aatttcatcc
      301 agatactgcc gatctcgacg agcacgaaat aggcaatacc gaaagcaata aatgtccctg
      361 aaacgcccag gcttttaagc agttcaccac ccccgagcgg accgagtttg ccggccagtg
      421 tcatccagag tgtggcgccg aatccgaatc cggcgacagc cagacccgta attagccctt
      481 tcttgtcggg aaaccattta atgcctaccg caataggaac gacatacccg atcccgatcc
      541 cggctccacc gataattccg acaaaaataa aggttgtggt aaagttttct gcgccgaata
      601 ttcctgccag cacatatccc aggccgagta cgagtccgct ggccatagcc agcctgcgag
      661 gccccacttt cggatgttgc aggaaccggc cggctactac catgacaatg gcaaacaaag
      721 ccagtccggc cgaaaagacc atctgtgttt cctgcttgct ccatccggaa ttttccagcg
      781 ttttcgtgta aaccgaccag gcataaatag cacccagggc aagttgaatc ataatagccc
      841 cgataaccac cagccagcgg ttcattgttt taatatctga cataaggatt ttggtttttg
      901 gttatgattt ttaaatgata accactaagg caccaaggca ctgagaagca ctaagttttc
      961 caattgtaaa ccgtactttg tgttcctttg tgatatcctt tgtgtcctta gtggttaacc
     1021 aaccgataga ttaccgtttc acttcaacct tggcgccgcg gatgatttct tccacaacac
     1081 ccggatcgag cagggtagag gtatcgccga ggctggtggc atcaccttcc ccgatcttgc
     1141 ggaggatacg gcgcatgatc ttccctgaac gcgtcttggg aagcccgctc acgatctgga
     1201 tcttgtccgg tttggcgatt ggcccgatga tccggctgac gatgtcgcat atctctttgt
     1261 cgatggtctg gacttcctct tccgaaagcg catcacaggt tacataggca tagatgcctg
     1321 aaccctttat ttcatgagga tagccaacga cagccgattc caccaccttc gggtgctggt
     1381 tgatggcgtc ttccacttcg gccgttccga tgcggtggcc cgaaacattg ataacgtcgt
     1441 cgacacggcc caggatgcgg tagtagcctt cctcgtcgcg tttggcgccg tcgccggtga
     1501 aatagagccc gcggtaggtc gagaaatagt tgatccggca gcgctcatgg tcgccatagg
     1561 tcgtacggat catgcccggc cacgggaact tgatgcaaag gttaccctgt acattattcc
     1621 cctccatgac attgccttcg ttatcgacca gcaccggttg taccccgggc aatggcagtg
     1681 tggcaaacga cggtttggtc ggggtaatgc cggccagcgg cgtgatcagg atgccaccgg
     1741 tttccgtctg ccaccaggtg tcgacgatcg ggcatttgcc tttgcccacc acatcgtgat
     1801 accagcgcca tgcctcttcg ttgattggtt cgccaaccga accgaggact ttgagtgaac
     1861 tgaggttttt ggaacgcacc cactcgtctc cctgtgcgat cagggcgcgg atagccgtcg
     1921 gcgcagtata gaactggttg acctggtatt tgtcgaccgt ttcccagaac ctgcccggat
     1981 cgggataggt aggtactcct tcgaacatga ccgatgtggc gcccgaaagc aacggcccgt
     2041 aaacgatata ggtatggccc gttacccagc cgatatcggc tgtacaccag taaatatcgc
     2101 cttcattgta ctggaaaaca ttcaggaacg tgaactgggc aaataccatg tatccggctg
     2161 tggtatggag aacgcctttc ggtttgcctg ttgatccgga ggtataaagg ataaacagcg
     2221 tatcctcggc ttccatgact tcggccttgt tttctttcga aacaccttcg ataaaggcat
     2281 cccaccagat atcgcgtcct tctttcatct ggaccgggtc gcccgtacgt ttcagtacca
     2341 cgacatgctc cacggaggtg cacatttgca gcgcttcgtc aacaatcgat ttcagtccga
     2401 cattcttgga accacggtac aaaccatcgg cggtaatgac cattttacag gcggaatcgt
     2461 tgatgcgatc ggccagcgag ttggcggaaa atccggcgaa gacaatgctg tggatggctc
     2521 cgatacgggc acaggccagc atcgctatgg gaagttccgg aaccattggc agataaatgg
     2581 ctaccctgtc gcccttctgg ataccttgcc ttttcagtgc attggcaaaa atgcaaacct
     2641 tttcgaataa ttccccgtac gtgatcctct tttccggttc tttgggatcg ttcggttccc
     2701 agatcagcgc cacctggtcc tttcgggcag gcagattgcg ctcaaaaata ttctccgtga
     2761 tattcagttt cccaccgtcg aaccacttga tcttgggctc ggtgaaattc caatccagca
     2821 ctttatccca ttttttgtgc cattggaacg tgtcggcgat ctttgcccaa tacccctcgg
     2881 gattggtcac gctttcatgg tattgttgaa aataatcaac aaaacttgaa attttcttca
     2941 tcatacgctt atgatttaaa tttgtaaata atgattagga tatttagatg ttattgggtt
     3001 tcaatttttt aaaagaaata aaatacacct acaagcaagc ggaaattacc gaccggttcc
     3061 ggatccataa ctttaccgtc gagattgttg gtcatgccgt atctggcaac cgtatattct
     3121 atctcagggg caatcctgaa tttgcctgca ttataaacga accggggtga tatccggtag
     3181 aggtcataaa tattcgctcc tcttgaatag taagggccta tcacaatatc gccggcaccc
     3241 atgttctgag aataggccag gaataaccct ccctgcattt tcttgcccgt cgtcatcacc
     3301 tcggcccaca ccgatgccgt ggtgatattc cggtagctga cgatcccttt gtctttgtcc
     3361 aggtaatcat ccaccacata acccccgatc atggtataat cgaacatgtt ctggccgtat
     3421 aatcctgcca ttttgaaaat gaccttctga aaacctaatt tcaaatatac catccccgaa
     3481 agggaattaa taccttcatt cgtctgatag ccactatcgg tgatcatcct cgggaggagc
     3541 cgtttgaaat taccggcggc gcctgccagg atctccgtgc cggtttcctt gtttttatat
     3601 gaatactcaa agttgaggtt aaaagagggt atggaggaat ttcggatgta tttcgtgctg
     3661 gctccgtcgg ggccgttgct tttaaaatcg acctgtgaca gggcagtgag aatcaggcga
     3721 aaacttttaa tgtcttttgt aagtctgatc tgaggattgc gggaaaagac caggaaaggg
     3781 gcaccggtat tgaaagaaac cacctccggg aatgaggaag tgataaacat cgggtgccac
     3841 atctgcccga ccagcagctc ggtagttttc cagttcagct ttacgtaggc atgacgcagg
     3901 cggaaaccgt taatatcacc atcggccata ccgaaaaatt cgccttcgat cgatcccgag
     3961 gtttttgcac ccagtgcgtc agggcctgtt atatctccct tgagccgggt ctgtatgctg
     4021 aggaaattaa aactcggctt ggcattgata tcgttgcctt ccaggtcgtt taatactggt
     4081 ttgggaagca aaagaaagtg cccttccctc aaatcgaccg tttgccgcga atcccagatg
     4141 acatctgttt taacgaaccc ggtaaacttt attccaaatg ttttatcatc ctgcgagaat
     4201 aacatgacag gaaagtaagc caggaaaagt aaaaggagag gtttcttcat tcgggatttt
     4261 cttgttaatt gattaacaat aaaagattta ttacgggcga agatagaatt ttttttgact
     4321 taaacaagcc attagcagga agggaagata ggtatccgaa atagtaaaga tcggatattc
     4381 aatacattat tatatagttt aaaaactata tgaattaatt accccgaaat ttaaaagcat
     4441 tataaataag aagcgtgtag cgccgtttta ccggtttggt tatcagtctt caagctgatc
     4501 gac
//
LOCUS       DAOQXS010000225         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19691_length_4495_cov_3.567342, whole genome shotgun sequence.
ACCESSION   DAOQXS010000225 DAOQXS010000000
VERSION     DAOQXS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19691_length_4495_cov_3.567342"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..3916)
                     /gene="purL"
                     /locus_tag="PKM34_07125"
                     /gene_synonym="purI"
     CDS             complement(83..3916)
                     /gene="purL"
                     /locus_tag="PKM34_07125"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNQ83398.1"
                     /translation="MILFYQNNHSVIYAIGSKIHQPKKDLEKLAWLLGDASLVMWEQV
                     EGQFIGPRKEMITPWSTNAVEITQNMGIEGIERIEEFRYFKEGDSFDPMLQHLYKGLD
                     QKIFTIEHKPEPILYIDDIRAYNLKEGLALSEEEVIYLEGLAEKLGRKLTDSEVYGFA
                     QVNSEHCRHKIFNGNFIIDGDVKEDSLFSMIKNTSKVNLNRLVSAYKDNVAFISGPAA
                     QEFAPETQDKADFFRIRPVNTVISLKAETHNFPTTVEPFNGASTGTGGEIRDRMAGGK
                     ASIPLAGTAVYMTSYPRMAPGRPWEERLKPRKWLYQTPEEILIKASNGASDFGNKFGQ
                     PLICGSVLTFEHEEDGKSYGYDKVIMLAGGIGTGIREDSIKDIPGTGDLIVVMGGDNY
                     RIGMGGGAVSSVATGEYGNAIELNAVQRANPEMQKRVYNAIRAMAESGANPVISIHDH
                     GAGGHLNCLSELVEATGGTIDMTKLPVGDPTLSDKEIIGNESQERMGLIISEKDLPLL
                     KRIAERERAPLYVVGRTTGDHILSFVNAQGEKPMHLELANLLGKPPRTVLNDTTLPAS
                     FADPAYTVVKTQHAVVETQHAASLSHATVKTQHAASLPIALHHVLQLEAVACKDWLTN
                     KVDRSVTGRIAKQQCAGTVQLPLNNLGVATMDYLGKRGIATAIGHAPVAGLIDPEKGS
                     VLAVAEALTNIVWAPLEDGLKGVSLSANWMWPAKNPGEDARLFAAVKKVSEFAIGLGI
                     NIPTGKDSLSMTQKYPDGQKVFAPGTVIISAVGEVANVRQIVEPVLQPAPPVETLHAT
                     PPVETLHATSLQGAGCIIYIDFSGDGFHLGGSSFAQSVNAIGTAVPGVPDTGYFARAF
                     ETVQNMIREEMILAGHDISAGGMITTLLEMTFGRNDAGMNLNMDAVGEKDIYRILFSE
                     KPGVIIQVKDPDEASRLLEKEQIRFYRLGQVTGNRTIEIGHDGQSYLFDIEELRDTWY
                     KTSYLLDRKQCGPELAARRYENYKKQELSFRFPDSFSGRFAQYGIEQARIVKSGIKAA
                     IIREKGVNGDREMAWSMHLAGFDVKDVHMTDLIAGRENLEDVNLIVFVGGFSNSDVLG
                     SAKGWAGAFLYNPKAREALDKFYARHDTLSLGVCNGCQVMVELGLVYPEHARMPKMLH
                     NASGKFESAFLNVDVHETKSIMLKSLAGSRLGIWVAHGEGQFSLPYEEGKYEIAMKYS
                     YTGYPGNPNGSDYSAAALCSHDGRHLVMMPHLERAIHPWNWASYPASRPSDEVSPWIE
                     AFVNAKKWIETRCGD"
     gene            complement(3918..>4495)
                     /locus_tag="PKM34_07130"
     CDS             complement(3918..>4495)
                     /locus_tag="PKM34_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ83399.1"
                     /translation="VMLAWYQYTIGKKTGSLMLQANGKNMMGDVVISLAVLIGLVFTF
                     VMKMPVFDKITALIVSGWIIYLAFRIFRESSLQLMDGVDDESVYDRVFKAISKVEGAS
                     NPHRTRIRKIGHQYMVAVDIEVDGDMPLKMAHQLAHQVEKQIKLELTDVYDVMVHTEP
                     VGDDPAEEKFGISEDNFQKLKKTRRSKSKNT"
BASE COUNT          970 a         1250 c         1106 g         1169 t
ORIGIN      
        1 cgcgtcggcg aaccgttcga ccagctcgtc atcacatcga cgatcgcctg ttcgcataat
       61 cgcctgttcg cccgtacgct tgttaatccc cgcatctcgt ttcgatccat ttttttgcgt
      121 tgacgaatgc ttcgatccag ggtgagacct catcggaagg gcggctggcg ggatagctgg
      181 cccagttcca gggatggatg gcccgctcca ggtggggcat catgaccagg tggcggccat
      241 cgtgcgaaca tagcgctgct gctgaataat cgcttccgtt aggattgccg ggatagccgg
      301 tataactgta tttcatcgcg atctcatatt tgccttcctc atacggcagg gagaactgcc
      361 cttctccatg tgcaacccag atgcccagcc ggctaccggc aaggcttttc aacataatgc
      421 ttttggtctc atgaacatcc acattaagaa aagccgattc gaacttaccc gaagcattgt
      481 gcagcatctt cggcatgcgg gcatgctcag gatagaccag tcccagttcg accattacct
      541 ggcatccgtt acagacgcca aggctaaggg tatcatgacg ggcgtagaac ttatcaagcg
      601 cttccctggc tttaggattg taaaggaaag caccggccca gcccttcgcc gagcccaaaa
      661 catcgctgtt ggagaatcct ccgacaaaca cgatgagatt aacatcttcc aggttctcgc
      721 ggccggcgat aaggtcggtc atatgaacgt cttttacatc gaacccggcc aggtgcatgc
      781 tccaggccat ctcccggtcg ccgttcaccc ctttctcgcg gataatcgcg gctttgatgc
      841 ccgactttac aatccttgcc tgctcaatcc catattgagc aaatctgccg ctgaaagaat
      901 caggaaaccg gaacgacaac tcctgctttt tgtaattttc gtatcttctg gcagctagtt
      961 ccggtccaca ctgcttacga tcaagcagat aggatgtttt gtaccatgta tccctgagtt
     1021 cctcaatatc aaacaaatag gattgtccat catggccgat ttcaatcgta cggttgccgg
     1081 taacctgacc gagacgataa aatctgatct gctctttttc aagtaaccgt gatgcttcgt
     1141 ccgggtcttt cacctggatg atcacaccgg gtttttcact gaaaaggatg cggtagatgt
     1201 ccttctcgcc gacagcatcc atgtttagat tcatcccggc atcattccgg ccgaaagtca
     1261 tctccagcag agtggtaatc ataccgccgg cgctgatatc atgtccagcc aggatcatct
     1321 cttcacgtat catattctgc acggtttcga atgcccgcgc aaaatatccg gtatcaggca
     1381 cgcccggaac tgcggtgccg atggcgttaa ccgattgggc gaaactgctg ccccccaggt
     1441 ggaatccgtc gcccgaaaaa tcgatataaa tgatgcaacc ggccccctgt agagacgttg
     1501 catgcaacgt ctctacaggg ggcgttgcat gcaacgtctc tacaggcggc gccggttgca
     1561 acaccggttc cacgatctgc cggacatttg cgacctcccc tacggcggaa ataatgacgg
     1621 tacccggggc aaaaaccttt tgtccgtcag ggtatttctg tgtcatcgac aatgaatctt
     1681 tgccggtggg tatattgatc ccgaggccga tggcaaattc gctgaccttt ttaacggcag
     1741 caaacagcct ggcgtcctcg cccggatttt ttgccggcca catccagtta gcgctgaggg
     1801 atacaccttt caggccatct tccagcggcg cccagacaat attcgtcaga gcttccgcaa
     1861 cggccagcac agatcctttc tccggatcga tcagtccggc gactggtgca tggcctattg
     1921 ccgtagcgat tccccttttt ccaaggtaat ccatcgtcgc tacccccagg ttgttcaaag
     1981 gcagttgcac cgttccggca cattgctgtt tggctatcct gcctgtcacc gaccggtcga
     2041 ccttgttggt cagccagtct ttgcaggcga cggcttccag ttgcaggaca tggtgcaggg
     2101 ctatcgggag agacgcagca tgctgcgtct ttacagtagc atgcgataga gacgcagcat
     2161 gctgcgtctc cactacagca tgctgcgtct ttacaacagt ataggcagga tccgcaaacg
     2221 atgccggcag ggttgtatcg ttgagcaccg tcctcggtgg ttttccgagg aggttggcca
     2281 gttcgaggtg cataggcttt tcgccctgtg cattgacgaa ggataagata tggtcgccgg
     2341 tagtcctgcc gacgacgtac aggggagcgc gttctctttc tgcgatcctt ttcagcagcg
     2401 gaaggtcttt ttcgctgatg atcagcccca tccgttcctg tgattcattg ccgatgatct
     2461 ctttatcgga cagagtagga tcgccgacgg ggagctttgt catatcgatg gttccccctg
     2521 ttgcctccac cagttccgac aggcagttca ggtggcctcc cgcgccatgg tcgtggatgg
     2581 agatgaccgg gttggcgccc gattcagcca ttgcccggat cgcgttgtag acccgtttct
     2641 gcatctcggg attggcgcgc tgaacggcat tcagttcgat agcgttgccg tattcaccgg
     2701 tagccactga cgaaaccgcg ccgcctccca taccgatccg gtagttatca ccgcccatga
     2761 cgacgatcag gtcgccggtg cccggaatgt ctttaatact gtcttcgcgg attcccgtgc
     2821 caatgccgcc ggcgagcatg attaccttgt cgtagccgta agatttgccg tcttcttcgt
     2881 gctcaaaggt gagcacactt ccgcagatca gcggttgtcc gaatttgttg ccgaaatcgc
     2941 tggcgccgtt ggatgctttg atcaggatct cttcgggggt ctggtagagc cacttcctgg
     3001 gtttaaggcg ctcctcccag ggtctgccgg gcgccatcct gggatacgaa gtcatataaa
     3061 cggcggtgcc ggccagcggg atactggctt tgccaccggc catccggtcg cggatctctc
     3121 cgccggtacc ggtggaagcg ccgttaaacg gctcgacggt cgtcgggaaa ttatgcgttt
     3181 cggctttcag cgaaatcact gtgttgaccg gcctgatgcg gaaaaaatcg gccttgtcct
     3241 gcgtctccgg ggcaaattcc tgtgctgccg gtccgctgat aaaggctaca ttatctttat
     3301 aagctgagac caggcggttg aggttcacct tcgatgtatt ttttatcatc gaaaaaaggg
     3361 aatcctcctt tacatcgcca tcaataataa aattcccgtt aaagatctta tgcctgcaat
     3421 gctccgaatt aacctgtgca aatccgtata cttcgctgtc ggtcagcttc ctgcccaatt
     3481 tttctgccag cccttccagg taaatgacct cttcttcgct cagggcaagt ccttccttca
     3541 gattataagc cctgatatcg tcgatatata gtatcggttc gggtttatgc tcgatcgtaa
     3601 agatcttctg atccaaacct ttgtaaagat gctgaagcat ggggtcgaaa gagtcgccct
     3661 ccttaaaata gcggaactcc tcgatccttt caatgccttc gatacccata ttctgcgtta
     3721 tttcaaccgc attggtactc cagggagtga tcatctcttt ccggggtccg ataaactgtc
     3781 cttcgacctg ctcccacatg accagcgagg catcccccag caaccaggcc agcttttcaa
     3841 ggtccttctt cggttggtga attttgctgc cgatggcata aatgacagag tggttattct
     3901 gataaaaaag gatcatctca ggtatttttg gattttgacc tacgggtctt tttaagtttt
     3961 tgaaaattat cttcggaaat cccgaatttt tcttctgccg gatcatcgcc caccggttcg
     4021 gtgtgcacca tcacatcata aacatcggta agttcgagtt ttatctgctt ttccacctgg
     4081 tgagccaact gatgggccat tttgagcggc atatcgccat cgacttcaat atcgacggca
     4141 accatatact ggtgccctat tttacggatc ctggtacgat gtggattaga agccccttcg
     4201 actttcgata tcgctttgaa taccctgtcg taaacgctct catcatccac accatccatt
     4261 agctgcagcg aagattcccg gaaaattctg aatgccaggt aaatgatcca gccgctcacg
     4321 ataagagccg taatcttgtc gaaaacaggc attttcatca cgaaagtaaa aaccagtcct
     4381 attaaaactg ccagggatat gaccacatct cccatcatgt tttttccatt agcctgaagc
     4441 atcaggctgc cggttttttt accgatggtg tactggtacc aggcgagcat aactt
//
LOCUS       DAOQXS010000226         4493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19707_length_4493_cov_2.994142, whole genome shotgun sequence.
ACCESSION   DAOQXS010000226 DAOQXS010000000
VERSION     DAOQXS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19707_length_4493_cov_2.994142"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKM34_07135"
     CDS             complement(<1..463)
                     /locus_tag="PKM34_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83400.1"
                     /translation="MKTPKTFFFIVWLLFAINGVVAQNGSFIQKQLPCQVSHEPRFTV
                     TATVQDDDTWCKVEWPDPVGYYPELWYDDGEADDYFLFMNPGGMNALKFITYFDNFIV
                     TGGRIFVGDGSFPGPFLGTTFRVLVYDDDGSDGFPGTVIDSTDIMVENYGWV"
     gene            complement(601..1776)
                     /locus_tag="PKM34_07140"
     CDS             complement(601..1776)
                     /locus_tag="PKM34_07140"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNQ83401.1"
                     /translation="MKKIDDRLVTAAEKHVSVLLTDQLPETVLFHNLSYVQKLDKQAE
                     TIGKHEGLTEEEMNILRIGALFLYSGYTGGGDKVREKSAEIAAEFLVKEGIEPEAVDL
                     ISRIILSAGAEAKPEGLIAEALCDADLMFLASENAIEQFDLLFDECVMLRPGQEKRPV
                     YEKRWVDFLTSHQYYTNYGKTKLQDAKEETLRRLSDRMNRRRLIEARKESASEKRFSY
                     SRGVDTMFRITARNQINLNSIADNKSNILISVNAIIISIIITMLAGNIGNMSNDIIPI
                     LVLLVVCMVTITIAILSTRPNIVTSKFSGADIQNHQVDLNFFGNFIRMDYDEYLNAVR
                     DMMRDDEYLYSVLLKNQYELGKILSKKFRLVRIAYNVFMIGMIVTVVAFIVNYLFVH"
     gene            2201..3250
                     /locus_tag="PKM34_07145"
     CDS             2201..3250
                     /locus_tag="PKM34_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83402.1"
                     /translation="MMRITPAIILFLLFLTGAESFSQDKNAGKTVPLFESDDIIHLDL
                     RADFKAVFAATDDSTYFPAELIWQSDDGKNAAFDVEIRQRGETRRKKTTCSFAPLRLK
                     FPKNITDGTPFEDQKTIKLVTHCNKGDAFEQNIIAEYLVYKAFNVLTDSSFKVRPALI
                     RYISTGKKADTLQKFAFFIEREKHLAQRLKGDEIETEKVHPDRLDPFQTCLVDLFEYM
                     IGNTDYSIYERHNMVIVSNKTGQPPFISVPYDFDWSGLVSASYAVPNPKFNTSDVKVR
                     IYRGLKRDRETVDRAIRLFNERKQEIYRVFESYELLEQNERKQIARYLDEFYWTINNE
                     AQVRVEFFERARERE"
     gene            3308..>4493
                     /locus_tag="PKM34_07150"
     CDS             3308..>4493
                     /locus_tag="PKM34_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ83403.1"
                     /translation="MDNGQCTMDNVRLILFLLISGLLFPYVVFGMKDAEKTAVKAIRA
                     DDTTGLRIYMASHPGPNCEFSNGKTGLYYAIVYDAINSAEFLLAGGAEPDDTVDGNAL
                     LKWAIKYNRPRITRFLIEFGAGINTPDDFGNTPLMYAASVNNLEICRLLVNRGADPMI
                     KNPYGRRASDFATAESETPLFRYLLFMERMIMEQDSLPDMLDGPYIFQENDRRLTMKY
                     YERSEEMNRTFISEKTLTPVIQDLVVSGFGWDTNTYRITGSFDPDSCIFETTGDVFVI
                     GDIHGRYQALVNLLINNKIIDDELNWRFGNGHLVLLGDVFDRGDKVTETLWFLYGLQF
                     QARESGGNVHLLLGNHEIMAMTGDYRYLHEKYQFFMRYTSSYYDELYDENSLLGRWLR
                     SRN"
BASE COUNT         1255 a         1052 c         1090 g         1096 t
ORIGIN      
        1 ccacccaccc gtaattctca accattatgt cagtagaatc gatgacagta ccggggaagc
       61 catcgctgcc atcatcatca taaaccagta cacggaaggt ggtgcccaaa aatggaccgg
      121 ggaatgaccc gtcaccaaca aaaatccttc cgccggttac gataaagttg tcaaaataag
      181 tgatgaattt gagtgcattc atgcctccgg gattcataaa taaaaaatag tcgtctgctt
      241 cgccgtcgtc gtaccataat tcggggtaat agccgaccgg atcaggccat tccaccttgc
      301 accaggtgtc atcatcctga actgttgcag taacagtgaa tctcggttca tgtgaaacct
      361 ggcagggcaa ttgcttctgt ataaaactac cgttctgggc aacaaccccg tttatggcaa
      421 acagaagcca aacgataaaa aagaatgtct ttggcgtttt catcggtgaa ttggttaaca
      481 ttcataagac agaaaaatat atgaaatggt tgcatcagtg ttctttaaac cggaacacgg
      541 atgacacgaa tgggacggat aatcacgaat gggacggata atcccggata cgaaattttc
      601 ctaatgaacg aaaaggtaat tcacgatgaa agcgaccacg gtaacgatca tcccgatcat
      661 aaaaacattg taggcaatcc tcacaagccg gaacttcttc gacaggatct tccccagttc
      721 atactggttt ttcagcagta ccgaataaag atactcgtcg tctctcatca tatccctcac
      781 ggcattcaga tactcatcat aatccatcct gatgaaattg ccgaagaaat tcaggtcaac
      841 ctggtggttc tgaatatcgg cgccggaaaa tttacttgtc acgatgttgg gccgggtact
      901 caggatcgca atggtgatgg tgaccatgca gaccaccagc aacaccagga tcggtatgat
      961 atcattcgac atattgccga tattgcctgc cagcatggtg ataatgatgg agatgataat
     1021 agcgttgacc gagatcagga tgttggattt gttgtcggcg atggagttca ggttgatctg
     1081 gttacgcgcc gtgatacgga acatggtatc gacgccgcgc gaatagctga aacgcttttc
     1141 tgaagccgat tctttccggg cttcgatcag cctgcgccgg ttcatccggt cggacaggcg
     1201 cctcagcgtt tcctccttcg cgtcttgcaa cttggttttt ccgtaattgg tgtaatattg
     1261 gtgagatgtc agaaaatcga cccagcgctt ttcataaacc ggacgctttt cctgccccgg
     1321 gcgcagcatc acacactcgt cgaacagcag gtcgaactgc tcgatggcat tttcggatgc
     1381 caggaacatc aggtcggcat cacacaatgc ttcagcaata aggccttcgg gcttcgcttc
     1441 ggccccagcc gacaaaatta tccgggaaat caaatccacc gcctccggtt caatcccttc
     1501 ctttaccaga aattccgcag ctatttccgc gctcttttca cgaaccttgt ctccgccgcc
     1561 ggtatagccc gaataaagga acagggcacc tatccggaga atattcatct cttcttccgt
     1621 cagtccctca tgttttccaa tggtttcagc ctgcttatcc agcttctgaa catacgatag
     1681 gttatgaaaa aggaccgttt cgggtaattg atcagtcagt agaaccgaaa catgcttctc
     1741 agccgcggta accaggcggt cgtcgatttt tttcataaat aattgaggat aaatgaacaa
     1801 aagtaactga acaaagttat ctaattttga ggcacgaaac aaaatgctga catattcatc
     1861 cgacagatga gacctacggt tctgaacttg ttaaaacact ggaagagggt atctcaaaag
     1921 gatttaacca caaaggaaca ccaaggaatt cacaaaggca cacaaaggaa tcacgttgac
     1981 attcaacgac ttcgtgcgcc tttgtgttgt actttgcgcg cctttgtggt taattcagac
     2041 ccatttgagt aaaccctacc aattgttttg taaagatggg aaccgacggg aagctcgttg
     2101 gactacttat aaaaagtaaa gattaagact ttcgatgcaa cttaagattt aatcggaaaa
     2161 ctaacacaag atgacagatt taacctaatt taacagtacg atgatgagaa taaccccggc
     2221 gataatattg ttcctcctgt ttctaaccgg agcggaatcc ttttcccagg ataagaatgc
     2281 aggaaaaacg gtgcctttat tcgagtcgga cgacatcatt catctcgacc ttagggccga
     2341 ctttaaagcc gtttttgctg caaccgacga ttcaacctac ttcccggccg aacttatctg
     2401 gcaaagtgat gacggtaaga atgcggcatt tgacgtggag atccggcaga ggggagaaac
     2461 gcgccggaaa aagaccacct gttcctttgc cccgctgcgg ctgaaatttc ctaaaaacat
     2521 taccgatggc accccattcg aggatcaaaa aacgatcaaa ctggtgaccc actgcaataa
     2581 aggcgatgca ttcgaacaga atatcatcgc tgagtacctg gtatacaaag catttaatgt
     2641 actgaccgac agctccttca aggtccgccc ggccctgatc cgctatatct ccaccgggaa
     2701 aaaggccgac accctgcaga aattcgcctt ctttatcgaa cgggagaaac acctggccca
     2761 gaggctaaaa ggggatgaaa tcgaaacgga aaaagtccat cccgaccggt tggatccttt
     2821 ccagacctgc ctcgtcgatc tgttcgaata catgatcgga aataccgatt attccatcta
     2881 cgaacggcat aatatggtca ttgtcagcaa taaaaccggt cagcctcctt ttatctccgt
     2941 gccatacgat ttcgactgga gcgggctcgt ttcggccagc tatgccgtgc ccaaccccaa
     3001 attcaatacc agcgatgtca aagtacggat ctaccgggga ctgaaacggg accgggaaac
     3061 ggtcgaccgc gccatacggc ttttcaatga aagaaaacag gaaatttaca gggtgtttga
     3121 gtcgtatgag ctgctggagc aaaacgaacg gaaacagatc gccaggtacc tggatgaatt
     3181 ctactggacc atcaacaacg aagcccaggt gagggtggag ttttttgaaa gagcgaggga
     3241 gagggaatag tgaagagtga agagtgaagt gtgaagtgtg aagagtgaag agtgaaatac
     3301 atggacaatg gacaatggac aatgcacaat ggacaatgtt aggctcatat tgtttttgct
     3361 gatttccggc cttttgttcc cgtatgtggt ttttgggatg aaagatgcgg agaagacggc
     3421 tgtcaaagcg atcagggctg acgatacaac cggtttgcga atctacatgg catcgcatcc
     3481 gggacccaac tgcgagttca gcaacggtaa aacgggcctg tattacgcta ttgtttacga
     3541 cgcaatcaat tcggctgagt tcctgctcgc aggcggcgct gagccggatg ataccgtcga
     3601 cgggaatgca ttgctgaaat gggcaataaa atacaaccgg cccaggatca cccggtttct
     3661 gatcgaattc ggggccggga tcaatacccc ggatgacttt ggaaacacac cgctgatgta
     3721 tgcggcttcc gtcaataacc tggagatctg ccggttgctg gtgaacaggg gcgccgatcc
     3781 tatgattaaa aatccctatg gaaggagagc ttccgatttt gctacggcgg aaagcgaaac
     3841 cccgttattc cgttacctgc tgttcatgga gagaatgata atggaacagg attctctccc
     3901 ggatatgctt gatggacctt acatttttca ggagaacgac cgccggctca ccatgaagta
     3961 ctatgagcgg tcggaagaga tgaaccggac ctttatttcg gaaaaaacgt taacaccggt
     4021 tattcaggat ttggtcgtca gtggatttgg atgggataca aacacttacc ggataaccgg
     4081 ttcatttgat ccagattcct gtatttttga aacaaccgga gatgttttcg tgatcgggga
     4141 tattcacggc cggtaccagg ccttagtcaa tttattgatt aataataaga taatagatga
     4201 tgaattgaac tggcgtttcg gcaacgggca cctggtcctg ctgggtgatg tgtttgaccg
     4261 gggggataaa gtgaccgaaa ccctgtggtt tctttacggg cttcagttcc aggcgcggga
     4321 atcgggcggg aatgtccatc ttttgctggg caaccatgag atcatggcca tgacgggcga
     4381 ctaccggtac cttcacgaaa agtaccagtt ttttatgagg tacaccagca gttattatga
     4441 tgagttgtac gatgagaaca gcctgctggg ccgctggctg aggagccgca atg
//
LOCUS       DAOQXS010000227         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19761_length_4483_cov_3.478546, whole genome shotgun sequence.
ACCESSION   DAOQXS010000227 DAOQXS010000000
VERSION     DAOQXS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19761_length_4483_cov_3.478546"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKM34_07155"
     CDS             <1..240
                     /locus_tag="PKM34_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83404.1"
                     /translation="VWPNPASGIVDCRWSIVDGRGDFLLEMYDMYGIEIRKFLIPVSN
                     HEIQMNVENLRPGLYLVVLRNGGNIVGSSKLIISR"
     gene            complement(366..890)
                     /locus_tag="PKM34_07160"
     CDS             complement(366..890)
                     /locus_tag="PKM34_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNQ83405.1"
                     /translation="MISKKVEKAINEQIKKEEYSSRLYLAMASWCQKNGYPGAAAFLY
                     THSDEERMHQLKFVHYLNDRGGYAVLQDIEQPAFKGDSLLKMFQEVLKHEQYITASIN
                     DIYHVCMTEKDYTTANFLQWFITEQIEEENLVNGILDQMALAGSDKSGLFHIDKELGG
                     KAAPAGGGAKPSAT"
     gene            complement(1526..2413)
                     /gene="atpG"
                     /locus_tag="PKM34_07165"
     CDS             complement(1526..2413)
                     /gene="atpG"
                     /locus_tag="PKM34_07165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNQ83406.1"
                     /translation="MPSLKEVRIRIESIKSTQQITSAMKLVAASKLRRTQNAVQALAP
                     YASKLREILGNLSASIDNTEEAVYTTVRPVQKVLLLVVTSNRGLCGPFNGNIIKAAKA
                     HIEKNYGDINRNGHLDIMTIGKKGTDFFRKNKYNLVSDNHEIFDSLTFTNTTAMATAL
                     MADFAAKKYDRIEIIYNHFKNAGQQVITIDQYLPIIPVTTGKPADSKQKADYLFEPEK
                     KVLIRELLPKMLKVQFYKIILDSFAAEHGARMTAMSQATENASEILKQLKLSYNKARQ
                     SAITKELLEIVSGAEALKG"
     gene            complement(2413..3996)
                     /gene="atpA"
                     /locus_tag="PKM34_07170"
     CDS             complement(2413..3996)
                     /gene="atpA"
                     /locus_tag="PKM34_07170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNQ83407.1"
                     /translation="MAQIKPAEVSAILRQQLAGFKDEKALEETGTVLTVGDGIALVYG
                     LSNAQAGELVVFEKTGIKGIVLNLEVDNVGVVLLGPSTNLNEGDKVVRTGRISSIDVG
                     EGMLGRVINTIGEPIDGKGEIKGEKFEMPMERKAPGVIFRQPVNEPLQTGIKPIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQKEFYDAGKPVYCIYVAVGQKGSSVAQTVK
                     TLEDHGAMPYTIVISATASDPAAMQFYAPFAGAAIGEFFRDTGRPALVVYDDLSKQAV
                     AYREVSLLLRRPPGREAYPGDIFYLHSRLLERAARVISADVIAAKMNDLPDSLRDKVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETSLFNAGIRPAINVGISVSRV
                     GGSAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDPSTKAVIEKGKRNVEILKQEL
                     HETMPVEKQIAIIFCGTRGLVKNVPVPSIKDFEVEFLHHMEVNHPKVLQELKAGRLND
                     DDVKLMEQAAKDVAKKYEK"
     gene            complement(4011..>4483)
                     /gene="atpH"
                     /locus_tag="PKM34_07175"
     CDS             complement(4011..>4483)
                     /gene="atpH"
                     /locus_tag="PKM34_07175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNQ83408.1"
                     /translation="DMDLLLQLICDSKEFRHLLISPVIKPDKKTGVLDALLKDKVDEV
                     SRKFYHLLATKRREKFLEGIARAYVEKYKAFNNIITVEIRTAGPLGAPLREQIVKLIE
                     KREGVTVDLVEQTDPAIIGGFIVSTGNLRYDASLSTAIKKLKKEFEENLYIREI"
BASE COUNT          982 a         1104 c         1123 g         1274 t
ORIGIN      
        1 gtgtggccta atccagcctc agggattgtc gattgtcgat ggtcgattgt cgatggtcga
       61 ggggattttt tattggagat gtatgatatg tatgggatag aaatccggaa atttcttata
      121 ccagtatcca atcatgaaat tcagatgaat gtcgaaaatc tcaggcctgg attgtatctt
      181 gtcgttcttc gtaatggagg caacatcgtt ggcagttcca aactgattat ttcgagatag
      241 aagacgaacc ggcgagatga aatcctctgc gtaactttgc gcgtctttgc gtacctctgc
      301 gaaatagctt ttaaaactat cacgcaaagt ggcgcaaagt atccgcaaag ggtcgcaaag
      361 cacaattaag tcgcggaggg cttggctcca ccccctgccg gagcagcttt accaccaagc
      421 tccttatcga tatggaataa tcccgattta tccgatccgg ccagcgccat ctggtccaga
      481 atcccgttga ccaggttctc ttcttcgatc tgctcggtaa tgaaccactg caggaaattg
      541 gccgtggtat aatccttttc cgtcatgcag acgtggtaaa tgtcgttgat cgaggccgtg
      601 atgtactgtt catgtttgag cacctcctgg aacattttca gcagactgtc acctttaaaa
      661 gcaggctgct caatgtcctg cagcacggca taaccgccgc ggtcgttcag gtagtggacg
      721 aattttaact ggtgcatcct ctcttcatcg gaatgggtgt ataaaaacgc tgcagcgccc
      781 ggataaccgt tcttctggca ccaggatgcc atggctaaat aaagccggga ggagtactcc
      841 tcttttttta tctgctcgtt aatggctttt tcgactttct ttgatatcat ggttcggaga
      901 ttttaaattt tttgcaaaga tatttattat aaaagaatac agtggtcaga agacttaagg
      961 tagaatgtta aactgttcaa ttgttcaatt gttaaattga aaatcgtccg aattactatt
     1021 acggattact acttttaagg ggaagcaaga tagtagacca ggttctgcca ttgaatacct
     1081 gctttatttt catcaaaata cttatccatt gttaatacga acttgggata attgtcatta
     1141 atgcgttcta gtggagcgaa ttcttcaccc gggttgccag cacctgagca ttccctgagt
     1201 ttaaagaaat cccgagactg ctttttccca tccttcaatt tcctggatct catcaataaa
     1261 taaatatttc gattatgaac gtatatttat aaagatatga aatttatttc gactatgatc
     1321 gaaataaatt atcgcaatat aaaacgaaag actaatgatt taaaggctgt ctcaaaagcc
     1381 tgatatttca caataatgac ctgatattag ttcgtgctcc aacgactaaa gccgttggtt
     1441 cctatggttt tacgaaggtg ggaaccgacg gcttcagtcg tcggactaac aattaaaagt
     1501 gaatatttgt acttttgagc caccctcatc ctttaagcgc ttcggcgccg ctgacgatct
     1561 caaggagttc tttggtgatc gcgctctggc gggctttgtt gtaagaaagc ttaagctgtt
     1621 tgaggatttc cgatgcgttc tccgtagcct gcgacatagc ggtcatccgg gcgccgtgct
     1681 cggcggcgaa cgaatccaga ataattttgt agaactgcac cttcagcatc ttcggaagca
     1741 gctcccggat cagcaccttc ttctccggtt cgaagagata atcggctttc tgtttgctat
     1801 cagccggctt tccggtagtc accgggatga tcggcaaata ctggtcgatg gtgatcacct
     1861 gctgtccggc gttcttgaaa tgattgtaaa tgatctcgat ccggtcgtat ttttttgcgg
     1921 caaaatcggc catcaatgcg gtggccattg ccgttgtatt cgtaaaggtg agggagtcga
     1981 agatctcatg gttatcgcta accagattgt atttattttt ccggaagaag tcggtccctt
     2041 tcttcccgat ggtcataatg tccaggtggc cgttccggtt gatatcgccg taattctttt
     2101 cgatatgcgc tttggcggct ttaatgatat tgccgttgaa cggtccgcag aggccgcggt
     2161 tggaggtaac gaccagcagc aacaccttct gcacgggcct taccgtggta taaaccgctt
     2221 cttcagtatt gtcgatgctg gcgctgaggt tgccgaggat ctcccgtagc ttcgacgcat
     2281 aaggagccag cgcctgcacc gcattctgcg tccggcgcag cttactggcg gcaacgagct
     2341 tcatcgcgct ggtaatctgc tgggtcgatt tgatcgactc gatccggatc cgaacttctt
     2401 ttaaactcgg cattatttct cgtatttctt agctacatct ttcgctgcct gttccatcag
     2461 cttcacatca tcatcgttaa gtcggccggc tttcagctcc tgcagaactt tcggatggtt
     2521 cacctccata tggtgcagga attccacctc aaaatccttg atgcttggta cagggacgtt
     2581 tttcacgagg ccgcgtgttc cacaaaagat gatggcgatc tgcttttcaa ccggcatcgt
     2641 ttcatggagc tcctgtttca ggatctcgac gtttcttttt cctttttcga tcacagcctt
     2701 tgttgatgga tcgaggtcgg aaccgaattt ggagaaggct tccagttcgc ggtactgcgc
     2761 ctggtcgagc ttcaatgttc cggccacttt cttcatcgat ttgatctggg ccgagccgcc
     2821 gacgcgtgat accgagatac cgacgttgat agcgggacgt ataccggcgt tgaacaggct
     2881 ggtctccagg aagatctgcc cgtcggtaat ggagatcacg ttggtcggga tgtatgcgga
     2941 gacgtcgccg gcctgggtct cgatgatcgg gagggcagtg agtgaaccac cgcctttgac
     3001 tttatcccgg agggagtcgg gcaggtcgtt catttttgcg gcgataacat cggctgagat
     3061 aacgcgcgcg gctctttcca gcaatctcga gtgcaggtag aaaatatctc ccgggtaagc
     3121 ttcccgtccg gggggccttc tgagcagcag tgagacctcg cgataggcca ccgcctgttt
     3181 cgacaggtcg tcgtacacca cgagggccgg ccttccggta tcgcggaaaa attcgccgat
     3241 ggcggcgccg gcaaacgggg catagaactg catggctgcc gggtcggaag cggtcgccga
     3301 gatgacgatc gtgtaaggca ttgcgccgtg gtcttcgagt gtcttcaccg tctgggcaac
     3361 ggaggagcct ttttgtccga ctgcaacata aatgcaatag accggctttc cggcatcgta
     3421 aaactctttc tggttgatga tcgtatcgat ggcgatggcg gtttttcccg tctggcggtc
     3481 gccgatgatc agctcgcgct ggccgcggcc gatcgggatc atggcatcga tcggcttgat
     3541 accggtttga agcggttcgt tcaccggctg gcggaagata accccgggag ctttgcgctc
     3601 catcggcatc tcgaacttct cccctttgat ctcgcctttg ccgtcgatcg gctcaccgat
     3661 ggtattgatg accctgccga gcattccttc gccaacgtcg atggaggaga tgcgtccggt
     3721 ccggaccact ttatcgcctt cgttcaggtt tgttgaaggt ccaagcagaa ccaccccgac
     3781 attatcgacc tccaggttta acacaatacc tttgatgcct gttttttcga agacgaccag
     3841 ttcgccggcc tgggcgttgc tgagcccgta aaccagtgca atgccgtcgc ctactgtcag
     3901 gaccgtccct gtttcttcca gtgctttttc gtctttgaat cctgccagtt gctggcggag
     3961 gatagccgat acttcagcag gtttgatctg tgccataaat cgctgttttg ctaaatttcc
     4021 ctgatatata aattctcttc aaattctttt ttcaatttct tgatggcggt cgacaggctg
     4081 gcgtcgtagc gcagattgcc tgttgaaaca atgaatccgc cgatgatggc cgggtcggtt
     4141 tgttctacca ggtcgactgt caccccttcc ctcttctcga tcagcttaac gatctgttcg
     4201 cgcaaaggtg ctccgagcgg gccggcagtc ctgatctcca ccgtgatgat attattgaaa
     4261 gccttgtatt tctccacgta ggccctggca atcccctcca ggaatttttc ccttcttttt
     4321 gtggccagga ggtggtagaa tttcctggaa acctcatcga ccttgtcttt cagcaaggcg
     4381 tcgagtacac cggttttctt gtccggtttg atcaccgggc taatcagcaa atgcctgaac
     4441 tctttgctgt cgcaaatcaa ttgcaacagc aggtccatat cgg
//
LOCUS       DAOQXS010000228         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19807_length_4475_cov_4.249321, whole genome shotgun sequence.
ACCESSION   DAOQXS010000228 DAOQXS010000000
VERSION     DAOQXS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_4475_cov_4.249321"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..793
                     /locus_tag="PKM34_07180"
     CDS             131..793
                     /locus_tag="PKM34_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83409.1"
                     /translation="MKLMLITRYSAVKPPVLLFNGSIVKEIDFNDPGEYLKTAYRRLN
                     LSYPKFFKMDNLCKLAFLTTEILLKDFDLNSRYPSEKTGLIILNSSSSLETDEKHQES
                     ISDRNNYFPSPSVFVYTLPNIMTGELAIRHKIKGENSVFITEKPDAEFLYGQVSELFG
                     SGRVNCCIAGWVEAYHEKLTSCLFLVENIEMADIEPKEIIIFDPSNIERIFKETTEDG
                     TY"
     gene            780..1034
                     /locus_tag="PKM34_07185"
     CDS             780..1034
                     /locus_tag="PKM34_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNQ83410.1"
                     /translation="MELIEKLKLEIIEVLNLEDIKPEDIDADAPLFGEGLGLDSIDAL
                     ELIVLLEKNYGIKIEDPKEGRKIFASVRTMADYILEHQKK"
     gene            1038..1586
                     /locus_tag="PKM34_07190"
                     /pseudo
     CDS             1038..1586
                     /locus_tag="PKM34_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000301.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
     assembly_gap    1587..1596
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1597..2284
                     /locus_tag="PKM34_07195"
     CDS             <1597..2284
                     /locus_tag="PKM34_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNQ83411.1"
                     /translation="HHGLLDRVLAGGSDSLSKFTLNGFNALMILDKHHCRPFDKSRTG
                     LNLGEGAAFLVLESEEALGSRTPLCELTGYANANDAYHQTASSPDGYGPFLSMSDALQ
                     TASKNIPGFNLRDISYINAHGTGTDNNDLTEGIAIGRIFGDAIPPVSSTKPFTGHTLG
                     AAAAVEAVISILSIRHNTIFPNLNFNEKIEELKFEPVREMITGVTLKHVLSNSFGFGG
                     NDSTLIFSAC"
     gene            2286..3353
                     /locus_tag="PKM34_07200"
     CDS             2286..3353
                     /locus_tag="PKM34_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNQ83412.1"
                     /translation="MEAYIRGTGNISPQATFDKALFLEEVRTYDTARLQVVEPDYKQF
                     IQPIQLRRMSRLLKMGVTAAGICLRDAGIEKPDAIITGTGLGMMEETEKFLNNLIDNQ
                     ERLLNPTAFIQSTHNTVGAHIAVMLGCNHYNLTYVHGPVSFENALLDSLMRIAEDPAK
                     NVLLGGIEEITMTHFVITGSMGFWKKSLTNTLDLLKDKDPGTAAGEGAAFFLLTATPD
                     EHCYAKVKGLAIRFNPDYGTVAPGFVQEFLAQHGLSPQEIDLAILGLNGDPDSDAVYG
                     PLLESFGSKVPAAYYKHLCGEHYLAASFALWLGAKIMKHGKVPDVIRLAQSNAGGQVR
                     HILIHNHYKNLHHSLILLSSC"
     gene            3404..4414
                     /locus_tag="PKM34_07205"
     CDS             3404..4414
                     /locus_tag="PKM34_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNQ83413.1"
                     /translation="MAGKTEILLYQTEDGQSKVEVRLENETVWLTQAQMVELFQTTKQ
                     NISLHVRNIFQEGELEEDLTVKDYLTVQTEGNRQVQRTIRYYNLDVIISIGYRVKSHR
                     GTQFRIWATQQLKEFLIKGFVLNDERLKETGYTNQYFDELLERIRDIRSSEKIFYAKI
                     KDIYTTSIDYSPDSRQALLFFASVQNKMHWAIHGHTAAEMIYHRANSDNPNMGLTSWK
                     NGPKGKIRKPDVSVAKNYLSHDELIELNLIVDQYLSFAELQARNRKPMYMADWEKKLH
                     DFLTLNEKQILLDAGRISHQMAVELAEKEFDKFNKKQIESDNRLTIKSLEIELKKLGK
                     KI"
BASE COUNT         1227 a         1203 c         1082 g          953 t
ORIGIN      
        1 gcgctcgcgt cattaagaaa aggtcattag ctcgctatcg ctcgcgtcat aaagttgtca
       61 taaggttgtc ataaggttgt aacctggaac gctggaacca tttttcttat ctttgcctgc
      121 tctgaagaaa atgaaactca tgcttatcac ccgctacagc gctgttaaac cgccggtttt
      181 gttgttcaat ggttctattg ttaaagagat tgattttaat gatccgggcg aatatttgaa
      241 aactgcatac cgccggctca atctctccta tccgaagttc ttcaaaatgg ataacctctg
      301 taagctggcc ttcctgacaa cggagatcct cctcaaagat ttcgacctga actcccgtta
      361 cccttccgaa aaaaccggcc tcatcatcct taattcctcc tcctcgctcg aaaccgacga
      421 aaaacaccag gagagcatct ccgaccgcaa caattacttc cccagcccgt cggtcttcgt
      481 ctatactttg cccaatatca tgacgggcga actggcgatc cgccataaga tcaaagggga
      541 gaattcggtt ttcatcacgg aaaaaccgga tgcggaattc ctctacgggc aggtcagcga
      601 gcttttcggc agcggccggg tcaattgctg catcgccgga tgggtggagg cttaccatga
      661 aaagctgact tcctgccttt tcctggtcga aaatatcgaa atggcagata tagaaccaaa
      721 ggaaataatt atctttgacc cctcaaatat tgaacgaatc tttaaagaga caacagagga
      781 tggaacttat tgaaaagctt aagctggaga tcattgaagt cttaaacctg gaagatatta
      841 aaccggagga tatcgacgcc gacgcgcccc ttttcggcga agggctgggg ctcgattcga
      901 tcgacgcgct ggaactcatc gttttacttg aaaaaaatta cggtatcaag atcgaagatc
      961 cgaaagaagg tcggaagatc tttgcttcgg tccggacgat ggccgattac atcctcgagc
     1021 accaaaaaaa atagtccatg gcgggccggg tttttgttac gggcatcggg atcatctgtg
     1081 ccatcggtaa aaataccggc gaaacgctgg cctctctgaa ggcctgccgt cacggcatcg
     1141 gaaagatcac cgttctcgat acggtccacc gcgacgaact gccggccggc gaaatcaagc
     1201 tgaccaatac agaactggcc gctcttgccg gcgttaccga catcgatatg aacccccgca
     1261 ccgccctgct gggcatgata gcggctgccg aggcgctgca aaatgcgggc atcgacccga
     1321 aagacaaccg tttccgcacc ggcgtgatct ctgccagcac tgtcggcggc atggacaaga
     1381 cggagatcaa ctaccggcat tacgaaaaag gcaaaccgta caacaacttc gtcctcaccc
     1441 acgactgcag ctacagcacc gagcagatcg cggcccggct gggatttcgc gatatggtga
     1501 gcaccgtcag caccgcctgc tcctcctccg ccaactcgat catgttcggg gccttgtaag
     1561 gcgaatcgaa attcagcggc aggcgcnnnn nnnnnnccac cacggcctcc tcgacagggt
     1621 gctggccggc gggagcgatt cgctgtcgaa gttcaccctc aacggcttca acgccctgat
     1681 gatcctcgac aaacaccatt gccggccgtt cgacaaaagc cgcaccggcc tgaacctcgg
     1741 cgaaggagcc gctttccttg tgctggaatc ggaagaggcg ctgggcagcc ggacgccgct
     1801 ctgtgaactc accggatacg ccaacgccaa cgacgcctac caccagaccg cttcgtcgcc
     1861 cgacggctac ggaccgttcc tgtcgatgag cgacgcgctg cagaccgctt cgaaaaatat
     1921 tcccggcttc aatctgcggg acatcagcta tatcaacgct cacggtaccg gcaccgacaa
     1981 caacgacctg acggaaggaa tcgccatcgg gcggatcttc ggggacgcca tccctcccgt
     2041 ctcctccacg aaaccgttca ccgggcacac cctgggcgcc gccgccgcag tggaagccgt
     2101 catctccatc ctgtccatac ggcacaacac catcttcccc aacctgaact tcaacgaaaa
     2161 gatcgaagaa ctgaaattcg aaccggtcag ggagatgatc accggcgtaa cgctgaaaca
     2221 tgtcctttcc aactcgtttg gatttggcgg aaacgattcc accctgatct tttcagcctg
     2281 ctgacatgga agcgtatatt cgaggcaccg gcaacatctc tccccaggca accttcgaca
     2341 aggccttgtt cctggaagag gtacgcacat acgataccgc ccggctgcag gtggtggagc
     2401 ccgattacaa acagttcatc cagccgatcc agctccgccg catgagccgc ctgctcaaaa
     2461 tgggcgttac ggccgccggg atatgcctcc gcgacgccgg catcgaaaaa cccgacgcca
     2521 ttatcaccgg tacgggcctg ggcatgatgg aggagacgga gaaattcctc aacaacctga
     2581 tcgacaacca ggaaaggctg ctcaatccca cggctttcat ccagtcaacc cacaacaccg
     2641 tcggcgccca tatcgccgtg atgctgggct gcaaccacta caacctgacc tatgtgcacg
     2701 gccctgtttc gttcgagaat gccctgctcg acagcctgat gcggatcgcg gaagatccgg
     2761 cgaagaacgt cctgctgggc gggatcgaag agataacgat gacccatttc gttatcaccg
     2821 gcagcatggg attctggaag aagagcctta cgaacaccct ggacctgctg aaggataaag
     2881 atccgggcac cgcggccggc gaaggggctg cgttcttcct gctgacggcc acacccgacg
     2941 aacattgcta cgctaaggtg aaagggctgg ctatccggtt caatcccgac tatggaacgg
     3001 tagctcccgg attcgttcag gaattcctgg cccaacacgg cttaagtccg caggaaatcg
     3061 acctggccat cctcggcctc aacggcgacc cggatagcga cgcggtgtac ggcccgctgc
     3121 tggaatcttt cggtagcaaa gtacccgcgg cttattacaa acatctttgc ggggagcatt
     3181 acctggcggc gtcgttcgcc ctctggctcg gggctaagat catgaaacac ggaaaggtcc
     3241 ccgacgtcat ccggctggcg caaagcaacg ccggcggcca ggtgcgccat atcctcatcc
     3301 ataaccatta taaaaacctt caccactccc ttatcctttt atcgtcatgc tgacaataat
     3361 gtgaaggcga aaaaattaat gtcatcaaat gctaaatata cagatggcag gaaagacgga
     3421 aatattattg taccagactg aagacggaca atctaaggtc gaagtccggc tggaaaatga
     3481 aaccgtatgg cttacccagg ctcagatggt tgaattgttt caaactacga aacagaacat
     3541 cagccttcat gtccggaata tatttcagga gggcgagctg gaagaagatt taactgtcaa
     3601 ggattacttg acagttcaaa cggaaggtaa tagacaggtt caaagaacaa tcaggtatta
     3661 caacctggac gtaattattt caattggtta ccgtgtaaaa tctcaccgtg gaacccagtt
     3721 ccggatctgg gcaacccaac agctcaaaga gtttttaatt aaaggttttg ttttaaacga
     3781 tgaacggctg aaagaaaccg ggtacacgaa ccaatatttt gacgaactgc tggaaaggat
     3841 aagagatatc cgcagttcgg agaaaatatt ttatgccaaa atcaaagata tttacacaac
     3901 cagcatagac tattcgcctg atagcagaca ggcgctgcta ttttttgctt cggttcaaaa
     3961 caaaatgcat tgggctattc acgggcacac cgcagctgaa atgatatacc acagagctaa
     4021 ctcagataat ccaaatatgg gtctgacttc atggaaaaat ggccccaagg gaaagatcag
     4081 gaaaccggat gtttctgttg ctaaaaacta tttatcgcat gatgagctga tagaattaaa
     4141 cctgattgta gaccagtacc ttagctttgc cgaattacag gctcgtaacc gaaaacctat
     4201 gtatatggcc gattgggaaa agaaattgca tgattttctt acgctgaatg agaagcagat
     4261 attactggat gcagggagaa tttcccatca aatggctgtg gaattggcag aaaaagagtt
     4321 cgacaaattc aataaaaagc aaattgaatc agataacagg ctaacaataa aaagccttga
     4381 gatcgaattg aagaaattgg gtaaaaagat atagctatta tcttgatagt caaaaaggaa
     4441 agtgaatcat ttcccacaaa tacacaacag aatca
//
LOCUS       DAOQXS010000229         4471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19840_length_4471_cov_3.879076, whole genome shotgun sequence.
ACCESSION   DAOQXS010000229 DAOQXS010000000
VERSION     DAOQXS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19840_length_4471_cov_3.879076"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PKM34_07210"
     CDS             <1..1269
                     /locus_tag="PKM34_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83414.1"
                     /translation="GSVWAALTVQLSPFASFSLPEMAGRVYPEALMMAPLLLIIVMVI
                     KNTFEPEKQHNLQKDLILYALIIGFGAAVKLTFVPLLLLPLLLLKTSLREKLRYFAYS
                     VLFFLIFAYPLLFNIGDSWDWITGIMVHSGKYGTGERNFIDISAAGMNARNLLEMNSG
                     FFYLVTASLLIIMVLSLNPVKKRLFPDQRIIRAILAIDLVVIACMAPILKHYSLYYFL
                     PFTIFKFPVFLLLTTLLLRIKVVAETRPFRLIIVLLAFVYFVFTLAVQVGQFRTHAAI
                     NTAKHAKTIENARKLVSVIDPGKPIIIASPYYGSPFIEFAHYNGYIMSRHMKGFYTDY
                     LREKFPGCYMSPTWSENFYSWDNFVDFKDILATVKSSFYIYIGCGMEKDLPVIKSRIS
                     QVLDMNSVMARTLYDDPDTGEQLIEIIVNQ"
     gene            1272..>1550
                     /locus_tag="PKM34_07215"
     CDS             1272..>1550
                     /locus_tag="PKM34_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HNQ83415.1"
                     /translation="MKIFYRYFLISFLLLFVSENGSSQAIYPRNYFRSPLEMRLLLSG
                     TFGELRSNHFHSGIDIKTAGTEGAPVYAIADGYVSRIKVSAYGFGKVLY"
     assembly_gap    1551..1560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1561..3046
                     /locus_tag="PKM34_07220"
     CDS             <1561..3046
                     /locus_tag="PKM34_07220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ83416.1"
                     /translation="GKVLYVTHPNGFVSVYAHLSKFSRIIGDHVKGEQYRRELFEIEL
                     FPEESRFPVKKGDIIAYSGNSGSSGGPHLHFEIRDAAQKTINPLLFGFEVKDFFKPRI
                     LSVKIYPENENSRVNGKNKPVRFLTEGWGTEHRITGNPAITVSGDISFAIQAYDQQND
                     NDNKNGAYAARLFIDSAKVFDFDVETFAFDETRYINSYLDYEEYVKNDVRLQRTKIDP
                     GNKLSMYNQLNNNGVYHFSDTLTHSIRYEVADVAGNTAVLAFKVKSVKPLLNKAETDT
                     TRTPGFDFPFSLFHWDRPNHFNNGSIVLDAPKGVFYDSFEFSVDSAAMIPGTYTRVYR
                     IHNKYTPVHDYMTLSIRPSDVPEELLAKALIVKVGDDGKSFSSAGGEWESGYIRTKIR
                     EFGNYTIAVDTVPPKVRAVNPGQFANMAGQKTLKLTVSDDLSGIASYRAVLNGKWILL
                     EYDPKNNLMIYTIDEHMVPGKNNLLVEVRDGKNNRFYYSAVLNL"
     gene            3088..3957
                     /locus_tag="PKM34_07225"
     CDS             3088..3957
                     /locus_tag="PKM34_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ83417.1"
                     /translation="MEGNIRDIQLNKLNERAKELKCIYQVCEALRDEKADLIKVFKSI
                     LDSIPPGYQYPTVCEARINFEGKQYSSTDFKETEWIQRAEIVIDNHISGEIQVCYIQD
                     VFEPNRRIFLPEEQKLLNTIADHLSRYIFQRRLKKTLELSLFPENGADYNGESSLLNY
                     ESDEHWKWRLQVAEKIAGMMDMERFGVEAVYLIGSTKSGQAGPGSDIDLLLHFRGKPA
                     QEKELRSWLEGWGLGLAELNYHKTGYVTSGSLIDLHILTDEAIKARSSYAVMIGSVEN
                     SARLLRSRKIRDD"
     gene            3950..>4471
                     /locus_tag="PKM34_07230"
     CDS             3950..>4471
                     /locus_tag="PKM34_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ83418.1"
                     /translation="MISCFFVSDLHGHTDRYEKLFGAILENKPQIVFFGGDLLPHGMR
                     RNLTDDFTRDFLIDRLLDIRSRMGSCYPRIYIILGNDDPRSEEKAFLNPEPEGLWFYL
                     HNRKDTCFGYTFYGYSNVPPTPFRFKDWERYDVSRYVDPGCIPPMEGFRTVEPEVDLE
                     FATIKDEIAMLIAD"
BASE COUNT         1156 a         1027 c         1079 g         1199 t
ORIGIN      
        1 gggtctgttt gggctgcgct gactgttcaa ctgagtccat ttgcctcttt ttcgctgccg
       61 gaaatggccg gaagggtata cccggaagcc ctgatgatgg ctcccctgct gcttatcatc
      121 gtcatggtca tcaaaaacac ctttgaaccg gaaaaacaac acaatctgca gaaagacctt
      181 atactttatg ctctgatcat cggattcggg gctgcggtga aactgacgtt cgtcccactg
      241 ctacttcttc ccctgttgtt actaaagaca tcacttcgtg aaaaactgcg atattttgct
      301 tattcggtgt tgttctttct cattttcgcc tacccgctct tatttaatat cggcgattca
      361 tgggactgga ttacaggaat catggttcat agtggaaaat atggcaccgg ggaaagaaat
      421 ttcattgata tctcagctgc aggaatgaat gcaaggaatc tgttagagat gaacagcgga
      481 ttcttttact tggtgacagc ttcactgctg attatcatgg ttttgtcatt aaatcctgtc
      541 aaaaaaagat tatttcccga ccagaggatc atccgggcca tactggccat tgatctcgtg
      601 gttattgcat gtatggcccc catccttaaa cattattcct tatattactt tttacctttc
      661 acaattttta aattcccggt atttctgctg ttgaccacat tactcttaag gataaaagtg
      721 gtagcggaaa caagaccttt ccgattgatc attgttcttc ttgctttcgt ttattttgtt
      781 tttacattgg cggtacaggt aggacagttc aggacgcatg cggcgatcaa cactgcaaaa
      841 catgcaaaaa ccattgaaaa cgccaggaag ctggtctccg tcatcgatcc cggcaaaccg
      901 atcatcatcg catctcctta ttatggctcc ccttttattg agtttgctca ttacaatggt
      961 tacattatga gccgtcatat gaaaggattt tacacggatt acctgaggga aaagttcccc
     1021 ggctgctata tgagtccgac ctggtcggaa aatttttatt catgggacaa ttttgtggac
     1081 ttcaaagaca ttttagctac ggtgaagtca tcattctata tttatatcgg ctgtggaatg
     1141 gaaaaggatc ttccggtgat caagagcagg atcagccagg tactggacat gaattcagtg
     1201 atggccagga cgctgtatga tgatccggat accggtgagc agcttatcga aatcattgtt
     1261 aatcaatagt tatgaagata ttttatcgat atttcctgat ttcatttctc ctgttattcg
     1321 tttctgaaaa cggttcctca caggcaattt atcccaggaa ttactttcgt tcacccctgg
     1381 aaatgaggct gctgctttcc ggaactttcg gagagctgag atccaatcac tttcattcgg
     1441 gtatcgatat taaaacagcc gggacggaag gggccccggt ttatgccatt gccgacggct
     1501 acgtgtcgcg gatcaaggtt tcggcttatg gctttggaaa agtattgtac nnnnnnnnnn
     1561 cgggaaggtc ctttacgtta cccatcccaa cgggtttgtt tcggtatatg ctcacctcag
     1621 caaattcagc cgtatcattg gcgaccatgt caaaggcgaa cagtaccggc gcgaactatt
     1681 tgagatagag ctgtttccgg aagaaagccg cttccctgtt aagaaagggg atatcattgc
     1741 ttattccggc aattcaggga gttcgggcgg gccgcacctc catttcgaga tccgtgacgc
     1801 tgcgcaaaaa accattaatc ctttgctttt tggttttgaa gtaaaagact tttttaaacc
     1861 ccgcatcctc tctgtcaaga tttaccctga aaatgagaat tcccgggtta acgggaaaaa
     1921 caagcctgtc cgctttctta ccgaagggtg gggaacggaa caccggatca ccggcaatcc
     1981 tgcgatcact gtcagcggtg atatatcctt tgccattcag gcttacgacc agcagaacga
     2041 taatgacaac aaaaacggcg cttatgccgc ccgccttttc atcgattctg ccaaagtctt
     2101 cgattttgat gtagagacct ttgcttttga tgaaacacgt tatatcaaca gttatcttga
     2161 ctatgaagaa tatgtgaaaa atgatgtcag gctgcaacgg actaaaatcg atccggggaa
     2221 taagctgagc atgtataacc agttgaataa caatggcgta tatcacttta gcgatacact
     2281 gacgcattca atccgttatg aggtggccga cgttgcgggc aacaccgccg tgctggcttt
     2341 caaagtgaaa tcggtcaaac ccttgctaaa taaagctgaa acggatacaa caagaacacc
     2401 cggtttcgat tttccctttt cccttttcca ctgggaccgg cctaatcatt tcaacaatgg
     2461 ctccattgtt ctcgatgccc cgaagggtgt tttttacgac tcctttgaat tttcggtgga
     2521 ttctgccgcg atgataccgg gaacatacac ccgggtatac aggatccata ataaatacac
     2581 ccctgttcat gattatatga cgctttcgat ccgtccgtcg gatgtccctg aagagctgct
     2641 ggccaaagcc cttatcgtaa aagtcggcga tgatggtaag agcttttcat cggccggcgg
     2701 ggaatgggaa agcggctata tccgcaccaa aatccgcgaa ttcggcaact atacgatcgc
     2761 ggtcgatacg gtaccaccaa aagtcagggc agtgaatccg ggccagtttg ccaacatggc
     2821 gggccagaaa accctgaaac tgacggtcag cgatgatctc tccggaattg cctcctaccg
     2881 cgccgtgctc aacggcaagt ggatcctgct ggagtacgat cctaaaaaca acctgatgat
     2941 ctatacgatt gacgaacata tggttcccgg gaaaaacaac ctgctcgtag aggtacggga
     3001 tggaaaaaac aaccggttct attactcggc tgtattaaat ttgtaaattc gtgcctgccg
     3061 gtttttaagc ctgtcaatat tatcgagatg gaaggaaata tcagggatat acagttaaac
     3121 aagctcaatg agagggcgaa agagctgaaa tgcatctacc aggtctgcga ggcgttgcgt
     3181 gatgaaaaag ccgacctgat caaggtcttt aaatccatcc tggattcgat cccgccgggc
     3241 taccagtatc cgacggtgtg cgaagcccgt atcaactttg aaggcaagca gtattcctca
     3301 accgatttta aagaaacgga gtggatccag cgggctgaaa tcgtgataga taaccatatt
     3361 tccggggaga tccaggtgtg ttatatccag gatgttttcg aaccgaaccg ccggattttt
     3421 ctgccggaag aacaaaagtt gctcaatacc attgccgacc atctcagccg gtatattttc
     3481 cagcgcagat tgaaaaaaac gctcgagctg tcgttgtttc ctgaaaacgg agcggattac
     3541 aacggcgaaa gttcccttct taactatgag tcggatgaac actggaaatg gcggttgcag
     3601 gttgccgaaa agattgcagg catgatggat atggaaaggt ttggtgtcga agccgtttat
     3661 ctgatcggca gcacgaaaag cggacaagcc gggccgggaa gcgacatcga cctgctcctt
     3721 cattttcgtg gcaaaccggc ccaggagaag gaattgcggt catggctcga gggatgggga
     3781 ttgggactgg cagagctgaa ttaccataag accggatatg ttaccagcgg aagcctgatc
     3841 gatctccata tcctgaccga tgaggccatc aaggcccggt cgagctatgc cgtaatgatc
     3901 ggatcggttg agaattctgc caggttatta cgttcgagaa agatccggga tgattagctg
     3961 cttctttgta tctgatctgc acgggcatac cgaccggtat gagaagctgt tcggggctat
     4021 ccttgaaaac aagccgcaaa tagtcttttt cggcggagat ttgctccccc acggcatgcg
     4081 cagaaacctg accgatgatt ttacccgtga ttttctgatc gaccggctgt tggatattcg
     4141 ctcccggatg ggttcctgtt acccgaggat atatatcata ctgggcaatg acgacccccg
     4201 gtcggaagag aaagcatttc ttaatcccga accggagggg ctgtggttct atctacataa
     4261 ccggaaagat acctgctttg gttacacttt ctacggatat tccaatgtgc cgccgacccc
     4321 tttccggttt aaggactggg agcggtacga tgtgtcgcgg tatgtcgatc cgggatgtat
     4381 cccgccgatg gaagggttcc ggacggtaga gcctgaagtg gaccttgaat ttgccaccat
     4441 aaaagatgag attgcgatgc tgattgcgga t
//
LOCUS       DAOQXS010000230         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19868_length_4467_cov_3.752493, whole genome shotgun sequence.
ACCESSION   DAOQXS010000230 DAOQXS010000000
VERSION     DAOQXS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19868_length_4467_cov_3.752493"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2418)
                     /locus_tag="PKM34_07235"
     CDS             complement(<1..2418)
                     /locus_tag="PKM34_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ83419.1"
                     /translation="MRNLFLLAAMLLLYPVLSISQTFIYSDNKDNQGFILLSSSDEKA
                     ELTFSVAAWSLETVTVNRAEMQKVTLPGHFLPNDEGMPDLPGSGRYMAIPQGSTASLV
                     IRNMETEILQNINMAPAPRIPKTTENGDLFYAINEQVYSTDAFYPAEPVKLSEAKQIR
                     GVDVVMLGITPFQYNPVTKELIVIKNLEIEVEFSGGNGHFGDDRLRSRWWEPVLADMV
                     MNYEELVPFGKISRRSAVGSQQPVGSSQQSAVGSRQAAGSRGETGFEYLIICPDDPAF
                     LAWADTIRVFRNKQGISTGVVTTTQTGGNSVNAIRTYINTAFNTWDIPPAAILILGDY
                     GTTGSTVVSPIWGNYCASDNIYGDVNNDDLPDIVMARIFAQNEAHLALMVQKFIDHEI
                     NPPVDPDYYDKPVTALGWQTERWFQICIETVGGFFRNVLGKEPVRINAIYDGDPNVDP
                     WSYAPNTSTILNYFGPNGLGYLPASPSTLGYWIGGTSGKISNAINNGTFMVLHRDHGY
                     EQGWGEPAYSNSSMASLDNEHTTFVYSVNCLTGKFNIRGECFAEGFLRYPKRAYGIIA
                     ASEVSYSFVNDTYVWGAMDHMWPEFMPEYGTTPETRGLYPAFSNAAGKFFLEQSSWPY
                     NTDNKEVTYHLFHHFGDAFTTIYSEVPQELLAVHNDVLIGGAATFRVHTEEGALAALS
                     VNGEIIGVADGTGEPQDIAIAAQQPGVVVDIVITKPNRFRYESKITVADPNTAYVVYD
                     NYLIQDDAGNGNGSADYNEEISLDLSLINLGLMDAENVSVTVSSDDPYVAVTDPEEQA
                     GDVPAGEV"
     gene            complement(2474..3553)
                     /gene="buk"
                     /locus_tag="PKM34_07240"
     CDS             complement(2474..3553)
                     /gene="buk"
                     /locus_tag="PKM34_07240"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNQ83420.1"
                     /translation="MEDVYIIAINPGSTSTKIAVYRNKECIFEENIKHSADDLAPFEK
                     ITDQFEFRKRVIMEKLNGTRIEKDKVRVIMGRGGLVYPIESGVYRINEKMKHDLVHSP
                     VGEHASNLGGFIAEDLAKDFPNAEAFICDPVVTDELDEIARIAGHPEFTRRSVFHALN
                     QKATARRHATAVGKKYEELNLIVAHLGGGISIGAHRHGRVVDVNHAIGGEGPFSPERA
                     GSVSVIDAVKLAYSGKYSEKEFTKMLIGKGGLCAHLGTNNAYEVEQRVKNGDEHAALV
                     YEAMAYQVAKFIGMKAVVLKGKVDGILITGGIAYDKWFVGKIREYADWIGPFFIYPGE
                     NEMEALAMNALRVINGEVEVKEYQN"
     gene            complement(3645..>4467)
                     /locus_tag="PKM34_07245"
     CDS             complement(3645..>4467)
                     /locus_tag="PKM34_07245"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNQ83421.1"
                     /translation="EHSLEAVAMAAKKKYIEAILVGNAAEIKRIADKNKKIDISQFEI
                     INETNDSEAVAKAVKLVHYGEADILMKGNVGTATLLKGVLNKEWGLRKNEVLSHIALF
                     EVQVYHHKLISLTDVAMNIAPDLNTKIGIINNSVEFLKKLGVETPKVAVLGAVELVNE
                     SMPATIDAALLSKMAERGQIKGCIIDGPLAFDNAISAESAKHKGIKSIVAGDADLLLL
                     PDIEAGNVLYKAFVFFAKAKVAAVVLGARAPIVLTSRSDSEESKLDSIVLAAAIA"
BASE COUNT          963 a         1201 c         1152 g         1151 t
ORIGIN      
        1 aacctctccg gcaggtacat caccggcctg ctcttccgga tcggtcaccg caacgtaagg
       61 atcgtcggat gatacggtta cgctcacatt ctcggcatcc atcaacccaa ggtttatcag
      121 cgacagatcc agcgaaatct cttcattata atcagccgat ccgttgccgt ttccggcatc
      181 atcctgaatt aaataattat catatacgac ataggccgta ttgggatctg ccacggttat
      241 cttcgattca taacggaaac gattgggttt cgtgatcacg atatccacaa caacgccggg
      301 ttgctgcgct gcaatagcaa tatcctgcgg ctcccccgtt ccatcggcca ccccgatgat
      361 ctccccgttg acactgagcg ctgccagtgc tccctcttcg gtatggaccc ggaatgtggc
      421 cgcgcctccg attaatacat cgttgtgaac agccaggagt tcctgcggta cctcggagta
      481 gatagtggtg aaggcgtcgc cgaaatggtg gaagagatga taggtaactt ctttgttatc
      541 cgtattatac ggccaggaag attgttccag gaaaaatttc ccggcggcat tgctgaatgc
      601 gggatacagc cctcttgttt caggtgtagt gccgtattcg ggcatgaatt ccggccacat
      661 atggtccatg gcgccccaga cataggtgtc gttcacaaag gaatacgaca cttcggaggc
      721 ggcgatgata ccgtaggccc gtttgggata gcgtaagaag ccctccgcaa aacactcccc
      781 gcggatattg aattttccgg taaggcaatt gaccgaataa acgaaagtgg tgtgctcatt
      841 gtcgagtgag gccatgctgc tgttgctgta agccggttcg ccccagccct gttcgtagcc
      901 gtgatcgcgg tggagaacca tgaaggtgcc gttgttgatg gcgttgctga tcttgcccga
      961 tgtgccgccg atccagtagc cgagggtgga ggggctggcc gggaggtaac ccagcccgtt
     1021 cgggccgaaa tagttaagga tggtgctggt attgggagca taggaccacg gatcaacgtt
     1081 gggatcgcca tcgtaaatgg cattgatccg gaccggttct ttgccgagca cattccggaa
     1141 aaagccgcca accgtttcga tacagatctg gaaccagcgt tccgtttgcc agcccagcgc
     1201 cgtaaccggc ttgtcgtagt aatccggatc gaccggagga ttgatttcgt ggtcaatgaa
     1261 tttttgcacc atcaatgcga gatgagcctc attctgtgcg aaaatcctgg ccattacgat
     1321 atcaggcagg tcgtcgttgt tcacatctcc atagatatta tcggaagcgc aataattccc
     1381 ccagatcggt gaaacgaccg tcgaacctgt cgttccgtaa tcacccagga tcaggatcgc
     1441 tgccggaggg atatcccagg tattaaaagc ggtgttgata taggtccgga tggcattgac
     1501 cgaatttccc cccgtttggg tggtggttac cacaccggtt gagatgccct gcttgttcct
     1561 gaatacccgg attgtgtcgg cccaggccag gaaggcggga tcatcggggc agatgataag
     1621 gtactcaaac ccggtttcgc ctctgctgcc tgctgcctgc cgactgccga ctgccgactg
     1681 ctgactgctg cccaccggct gctgactgcc gactgccgac cgccgactta ttttcccgaa
     1741 tggtaccaac tcctcatagt tcatcaccat atccgccagc accggctccc accagcggct
     1801 gcgaagcctg tcatcgccaa aatggccgtt accccctgaa aattcgacct ctatctccag
     1861 gttttttatg acgataagct ctttggtaac cgggttgtac tgaaacgggg taatcccgag
     1921 catgaccaca tccacgcccc tgatctgctt cgcttccgaa agttttaccg gttcggcagg
     1981 ataaaaagcg tcagttgaat agacctgttc gttgattgca tagaaaagat caccgttttc
     2041 agtggttttg ggaatccggg gggccggagc catgttgata ttctgcagga tttcggtttc
     2101 catgttccgg atcacaaggc tggctgtcga tccctgcggg atggccatgt accgtcccga
     2161 tcccgggagg tcaggcatcc cttcatcgtt tgggaggaaa tggcccggca gggtcacttt
     2221 ttgcatctct gcccggttga cggtaactgt ttcgagcgac caggcggcga cggaaaaggt
     2281 taattcagcc ttttcatccg aagaggaaag aagtataaat ccttgattat ctttgttatc
     2341 cgaatatata aatgtctgtg atatggataa aacaggatac agcaacagca ttgctgcaag
     2401 caggaacaaa tttctcatga aagacaggtt aagtgatgaa tcgcttatcg gggggtaaaa
     2461 ataataaaat cttttaattc tgatattcct ttacctccac ctctccgttg attacacgga
     2521 gggcgttcat ggcgagggct tccatttcgt tttcacccgg atagatgaag aacgggccga
     2581 tccagtcggc atattcgcgg atctttccga caaaccattt gtcataggca ataccgccgg
     2641 taatcaggat accatcgact ttccctttca gcacaaccgc tttcatgccg atgaatttgg
     2701 ctacctggta ggccatggct tcgtaaacca gtgcggcatg ttcatcgccg ttcttaacgc
     2761 gctgttcgac ttcgtaggcg ttgttggtgc cgaggtgtgc acaaagccct ccctttccga
     2821 tcagcatttt ggtgaattct ttttcgctgt attttccgct gtaagccagt tttaccgcat
     2881 caatcaccga aacgctcccg gcccgttccg gcgagaatgg accttcgccg ccgatggcgt
     2941 ggttcacatc gacaacccga ccgtgacggt gggctccgat gctgatcccg ccgccaaggt
     3001 gggctacgat caggttgagc tcttcgtatt ttttaccgac agcggtggca tggcgccggg
     3061 ccgtcgcttt ctggttcaac gcatggaaaa cggaccggcg ggtaaattca ggatggccgg
     3121 caatgcgggc gatttcatcc agttcatcgg ttaccaccgg atcgcagata aaggcttcgg
     3181 cattcgggaa atctttggcc aggtcttccg cgatgaatcc gcccaggttg ctggcatgct
     3241 cgccgaccgg gctgtgcacc agatcgtgct tcattttttc gttgatgcgg taaacacccg
     3301 attcgatcgg gtaaaccagc ccgccgcggc ccatgatcac ccgcacttta tccttttcga
     3361 tccgggtgcc gttgagcttt tccataatca ccctcttcct gaattcaaac tggtcggtga
     3421 tcttttcgaa cggcgccagg tcgtcggcgc tgtgcttgat gttctcttca aaaatacact
     3481 ccttattgcg atacactgct attttggtcg atgttgaccc cgggttgatg gctatgatgt
     3541 aaacgtcttc cacgaaagaa attcttaatt tttaattctt aatttttaat tcttgatttt
     3601 taattcttaa ttactgcaga ctgctgaccg ctagctactg actgtcaagc gattgctgcg
     3661 gcgaggacga tgctgtcgag cttcgattct tccgagtcgg agcgggaagt gagcacgatc
     3721 ggggcacggg cgccgagcac gacggcagcc actttcgctt tggcaaagaa aacgaacgct
     3781 ttgtaaagca cgttacccgc ttcgatatcg gggagcaaca gcaggtcggc atcaccggcc
     3841 acaatgcttt tgatcccttt gtgcttggcg ctttcggcgc tgatggcatt gtcgaaggcc
     3901 agcggcccgt cgatgatgca acctttgatc tgtccgcgct cggccatctt cgaaagcagg
     3961 gcggcgtcga tcgtcgccgg catgctctcg ttgaccagct ccacggcgcc gagaaccgcc
     4021 accttcgggg tttccacgcc gagcttcttc agaaactcca cggaattgtt gatgataccg
     4081 atcttggtat tcaggtcggg agcgatgttc atggccacat cggtcagcga aatgagcttg
     4141 tggtgataaa cctggacctc aaaaagggca atatgcgaga gaacttcatt cttccgcaat
     4201 ccccattcct tgttcagtac acctttcaga agagttgccg tgcccacgtt tcctttcatc
     4261 aaaatatccg cttcgccgta atggaccagc ttcaccgcct tggcaaccgc ttccgagtcg
     4321 ttggtctcgt tgatgatctc aaactggctg atatcgatct ttttgttctt gtcggctatc
     4381 cttttgatct ctgccgcatt ccctaccagg atcgcttcga tatacttttt ctttgccgcc
     4441 atcgcgactg cctccagtga atgttca
//
LOCUS       DAOQXS010000231         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19871_length_4467_cov_3.100408, whole genome shotgun sequence.
ACCESSION   DAOQXS010000231 DAOQXS010000000
VERSION     DAOQXS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19871_length_4467_cov_3.100408"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PKM34_07250"
     CDS             <1..163
                     /locus_tag="PKM34_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNQ83422.1"
                     /translation="QNQPQTAEYHKVVSGDTLSGIARRYGTTVTNLCTLNSISRETIL
                     QIGWRLRVK"
     gene            complement(240..1034)
                     /locus_tag="PKM34_07255"
     CDS             complement(240..1034)
                     /locus_tag="PKM34_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ83423.1"
                     /translation="MKLSSSPRLILGAVTGDIIGSVFEWNNVKTTDFQLFNPRTDFTD
                     DSVLTIATMDSILSGMDYAAAYQSYGRNYPGRGYGGYFSSWIHVPDPRPYNSFGNGSA
                     MRVSPVGWAYDNLDEVLYQAKRSAEVTHNHPEGIKGAQATAVAVFMARKGSGKGDIKK
                     YVQDTFGYDLERRIDDIRPGYRFNETCQGTVPEAIIAFLESNDYEDAIRLAISLGGDS
                     DTLACITGGIAEAFYKEIPGWIVKKVLQILPDELMAVIENFSKKFS"
     gene            complement(1040..2962)
                     /locus_tag="PKM34_07260"
     CDS             complement(1040..2962)
                     /locus_tag="PKM34_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ83424.1"
                     /translation="MKTLTLLFEESLRKYADNPYILEKKSTHYESFTFREIGEEVFKF
                     AAGLLCLGLKKGDRVALISESRADWVISELAVLHAGGVSVPLSVLIKEEADLKFRLEH
                     SGTRFVITSGNQAGKIIAAAKDLSSVEKIIFLDPVENKGVAGIYAGDLKQTGEEYLQE
                     HKDRLMEVIHSIGPDDHASISYTSGTTADPKGIILTHRNYTANIEQTLTLLDVPEWWT
                     TLLILPWDHSFAHTVGIYIMMATGSSFAAVQPGRSYLDSLKNIPGNIREIRPVFLLSA
                     PALVKNIRKGIEKGIRDKGKMTEKLFNHALRIAYKYNRTGWDRGKGMRIFLKPLYKLY
                     DLILFRKIRANFGGRLKFFFGGAALLDIEIQKFFYAIGIPVYQGYGLSEASPVISGNM
                     ADRHKLGSSGRVVGHLELKICDEEGRELPAKEKGEIVIRGENVMKGYWKNEKATREAL
                     RDGWLHTGDMGYLDKDGFLFVLGRFKSLLISDDGEKYSPEGIEETITDRSQLIDQLML
                     HNNQDKYTSALLVPNKEALMRWLKERNLAPAHPEGLETLLMEIESEINRYKPGGPFAD
                     LFPARWLPAAIAIIDEPFTIDNALLNSTGKVVRARVTEYHRDKFDFLYTPEGKNIVNA
                     RNIEALKKMLNPLDKI"
     gene            complement(2975..4183)
                     /locus_tag="PKM34_07265"
     CDS             complement(2975..4183)
                     /locus_tag="PKM34_07265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ83425.1"
                     /translation="MKTAFKWIIPLIITLAVPASCKKDEIGRPAVLNCLLEEDPVYRE
                     NPKSGRFQALIEEYVQKGLPGIVLLVKDDNGFYIGAAGMADIENDIKMQPCHVSKIAS
                     ITKMELGVAILRLQEKGILSLDDPISKYIDKDKLDKIANGNEPITVRNLMNHTSGIYE
                     VIEDAGFYLQILNDPARHWTADGLLKYVYNKEAMFAFDPEDTAGYCNTNFTLLSMIIE
                     AATGKPHSRVLHEEVIDLLGLSDTYYFWHDPLPDDRVAQGYYDLYNNGSIMNLSTWNT
                     GSGNGYGGVYSTVWDMYLFAEALFVEKSLLTQASLDQMLVFHPVVESRKLLGVACFKD
                     FIDIGDPEKDYSIGHRGRDLCYSADLDFFPEHNAIMTMIVNYGTDGDSPLRPVFLEMR
                     DKVAELIIED"
     gene            complement(4219..>4467)
                     /locus_tag="PKM34_07270"
     CDS             complement(4219..>4467)
                     /locus_tag="PKM34_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83426.1"
                     /translation="AFYLTDRSAMQDDGTYSSGMGFGKPQFIAGAGIGLGYDFGKDEQ
                     VRRIFVNYDFRLQMPFVSGYVPMLPNGSLGLGFQFNIN"
BASE COUNT          987 a         1202 c         1157 g         1121 t
ORIGIN      
        1 gcaaaaccag ccgcaaacgg cagaatatca caaagtggta agtggggata ccctatccgg
       61 aattgcaaga agatacggga ccaccgtcac caatctgtgt acgttgaaca gcataagccg
      121 ggaaaccatt ttgcagatcg ggtggcggct cagggtgaaa taacgagttc aaagttcaaa
      181 gtgcaaagtt gaactggctg aggtagcaat gaaaggcaat acggcccggc tgtagggttt
      241 caggagaatt tcttcgagaa attttcaatg actgccatca gctcgtccgg caggatttgc
      301 aagacctttt ttacgatcca gcctggtatt tccttgtaga aagcttcggc gatgccgccg
      361 gtgatacacg ccagcgtatc gctgtcgccg ccgagggaaa tggccaaccg gatggcgtct
      421 tcgtagtcgt tgctttcgag aaaggcgatg atcgcttcgg gcacagttcc ctggcatgtt
      481 tcgttgaacc ggtaaccggg ccggatgtca tcaattcttc tttccaggtc ataaccaaac
      541 gtatcctgga catacttttt aatatcccct ttaccggaac cctttcgcgc catgaacacg
      601 gcaacggcag tagcctgcgc tcctttgatc ccctccggat ggttgtgggt aacctccgcg
      661 cttcttttag cctggtacag gacctcatcc aaattgtcat acgcccagcc gacggggctc
      721 acccgcatcg ccgagccatt gccgaagctg ttgtaaggcc tgggatccgg aacatggatc
      781 caggagctga aataaccgcc ataaccacgt cccgggtaat tccgtccgta agattggtag
      841 gccgcagcat aatccatacc ggagaggatg ctgtccattg tggcaatggt gaggacgctg
      901 tcgtcggtaa aatctgtccg gggattgaat aactgaaaat cggtggtttt cacgttgttc
      961 cattcaaaga ccgacccgat gatatcgccg gtaacagcgc ccaggataag gcggggagag
     1021 gaggagagtt tcatttcttt taaattttat ctaatggatt aagcatcttc ttcagcgcct
     1081 caatatttct tgcattgacg atattcttcc cttcgggggt gtataaaaag tcaaatttat
     1141 cccggtgata ttcagttact ctcgccctga cgacttttcc cgtactgttg agcaaagcat
     1201 tgtcgatcgt aaagggttcg tcaatgatag cgattgcggc cggcagccac cgggcaggga
     1261 atagatcggc aaaggggccg cccggcttgt accggttgat ttccgactcg atttccatca
     1321 gaagggtttc caacccttca ggatgggcgg gtgctaaatt cctctccttc agccaccgca
     1381 tcagggcttc tttattcggg accaggagtg cggaggtgta tttgtcctgg ttgttgtgca
     1441 gcatcaactg gtcgatcagc tgcgatcggt cggtgatcgt ctcttctatg ccttccgggc
     1501 tgtatttctc cccgtcgtcg gaaatgagca ggctcttgaa ccggcccagg acaaaaagaa
     1561 agccgtcttt atccaggtaa cccatatccc ccgtgtggag ccagccgtcg cggagcgcct
     1621 cccgtgtcgc tttttcattc ttccagtagc ccttcatgac gttctccccc cggatgacaa
     1681 tctctccttt ctccttagcc ggcagttctc tgccctcttc atcgcagatc ttcagttcaa
     1741 gatgcccgac gacccggccc gaagagccga gcttgtgccg gtctgccatg ttcccggaga
     1801 tgacgggaga ggcttccgac aggccatagc cctggtaaac gggaatccct attgcataga
     1861 agaacttctg gatctctatg tcgagcagcg cggctccgcc gaaaaagaat ttcagcctgc
     1921 cgccaaaatt agccctgatc tttctgaaca gtattaaatc gtataatttg tacaacggct
     1981 tgagaaagat tctcatccct ttcccgcggt cccatccggt tctgttatat ttataagcaa
     2041 tccgcagtgc gtggttgaac agtttttcgg tcatttttcc cttgtccctg atcccttttt
     2101 cgatcccttt gcggatattc ttgaccaggg ccggcgcact cagtaaaaat accggcctga
     2161 tctcgcggat gttccctggg atattcttca ggctgtcgag gtacgacctg ccaggttgca
     2221 cggctgcaaa gctcgatcct gtggccatca taatgtagat ccctaccgta tgcgcaaaag
     2281 aatggtccca cggcaggatc agcagggtgg tccaccattc cgggacatcc agcagcgtca
     2341 acgtctgttc gatgttcgcc gtgtaattgc ggtgcgtgag gatgatccct ttcggatcgg
     2401 ccgtggtccc ggaggtatag ctgatgctgg catgatcatc cggcccgatc gaatggatga
     2461 cctccattaa ccggtctttg tgttcctgca ggtattcttc ccccgtttgc ttcagatccc
     2521 cggcatatat cccggctacc cctttgtttt caacaggatc gaggaagatg atcttttcga
     2581 ccgatgagag atctttagcg gcagcgatga tcttcccggc ctggttgccc gaagtgatca
     2641 caaaccgggt ccccgagtgc tccagcctga atttcaggtc ggcttcctcc ttgatcagaa
     2701 cagagagcgg gacgctcacg ccgccggcgt gcagcacagc gagttcactg atcacccagt
     2761 cggcgcgcga ttcggagatc aaagccaccc tgtcgccttt ttttaagccc aggcacagca
     2821 atccggccgc aaatttaaaa acctcttccc cgatctccct gaaggtaaaa gactcataat
     2881 gagtcgattt tttttcaagg atgtagggat tgtccgcata cttccggaga ctttcctcaa
     2941 aaagaagggt cagtgttttc ataggacagg cgttttaatc ttcgatgatc aattcagcca
     3001 ctttatcgcg catctccagg aaaaccgggc ggagagggct gtcgccgtcc gttccgtagt
     3061 tgacgatcat ggtcataatg gcattgtgtt ccgggaagaa atcgaggtcg gcgctgtagc
     3121 acagatcgcg gccccggtgg ccgatggaat aatccttctc cgggtcgccg atgtcgataa
     3181 aatccttgaa gcaggccact cccaaaagct tacgcgattc caccacagga tggaacacca
     3241 gcatctggtc gagcgaagcc tgtgtcagca gcgacttttc gacgaagaga gcttctgcga
     3301 aaaggtacat gtcccagacc gtgctgtaaa ccccgccgta accgttgcca ctgccggtgt
     3361 tccaggtcga caggttcatg atcgagccgt tgttgtaaag atcatagtat ccctgggcca
     3421 cccggtcgtc cggcagggga tcgtgccaga aataataggt atcgctcagc ccgagcaggt
     3481 cgatcacctc ctcatgcagc acccgggaat gcggtttgcc tgtagcggct tcgatgatca
     3541 tgctcagcag ggtgaaattg gtgttgcaat agcccgccgt atcctccgga tcaaaagcga
     3601 acatcgcctc tttgttgtaa acgtatttca gcaggccatc tgcggtccag tgccgggcag
     3661 gatcgttcag gatctgaagg taaaagccgg catcttcgat cacttcgtag attccgctgg
     3721 tatggttcat caggttcctg acggtaatgg gttcattgcc gttagcaatc ttatcgagct
     3781 tatccttatc gatgtattta ctgatcgggt catcgaggga aaggatccct ttttcctgca
     3841 accggaggat cgctaccccc agttccatct tggtgatgct ggctattttg gagacatggc
     3901 atggctgcat tttgatgtcg ttttctatgt cggccatgcc ggcggcaccg atatagaatc
     3961 cattatcgtc tttcaccagc aaaacaatgc ccgggagccc cttttggacg tattcttcga
     4021 tgagggcctg gaaccggccg cttttgggat tttcgcggta aaccgggtcc tcttccagca
     4081 ggcagttcaa aacggcaggc cttccgatct catctttttt gcacgatgcc ggcacagcca
     4141 gcgttatgat cagggggatg atccatttga aggcggtttt catagcatta cccgattttc
     4201 agttgtaaaa ggcaacattt aattgatatt gaactggaat cccagtccga gcgacccatt
     4261 cggcagcatg ggcacgtagc ccgacacgaa cggcatctgg aggcggaagt cgtagttcac
     4321 gaagatcctt ctgacctgtt catccttacc aaaatcatac cccagcccga taccggcgcc
     4381 ggcgatgaat tgcggtttgc cgaaccccat gccggagctg taggtgccgt catcctgcat
     4441 tgccgaccgg tcggtgaggt agaaagc
//
LOCUS       DAOQXS010000232         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19887_length_4465_cov_3.324490, whole genome shotgun sequence.
ACCESSION   DAOQXS010000232 DAOQXS010000000
VERSION     DAOQXS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19887_length_4465_cov_3.324490"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="PKM34_07275"
     CDS             <1..1805
                     /locus_tag="PKM34_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ83427.1"
                     /translation="YYSTEDLKNFRQWKSITPGHPELDVLHGIENTSGPLGQGHAMAV
                     GSAIAERFLAARFGEWMAHKTFAFISDGGIQEEISQGAGRIAGYLGLNNLVMFYDANN
                     VQLSTKIGEVTSEDTAMKYRSWNWYVQTINGNNAMEIREAISNALAEEQRPSLIIGKT
                     IMGLGAVDSQGNRMEGLTSTHGQPLSKAGVSLSDTIRKLGGDPDNPFEVSDEVKEYYR
                     LILSKKRIYTKDRDDQMTAWKDDNPDQALLWNNYTSGEVPDIDTEAIPIKPDAPTRNT
                     SGDVLSYFSKSIGNMIVMSADLSNSDKTEGFLKHSRPFQPGDLNGGFLHAGVSELTMA
                     AIANGIALHGGLFVVCGTFFVFSDYMKPAIRLAALMRLPVKYVFTHDSFRVGEDGPTH
                     QPVEHEMQLRLLEQLKNHRGHNSLLVLRPADSAETIISWKLALENTETPTALILSRQN
                     IKDIPAPAGGNRLKEAVHTRKGAYTVYEPDNEPDVILLANGSEVSTLIECAFLLKNDH
                     NIQARVVSAPSEGLFQLQDEHYRKSIIPDNTPVFGLTAGLPVTLKNLAGCKGKVFGLE
                     HFGYSAPFQVLDEKFGFNAQNIKAMVLDFLQEPI"
     gene            complement(1817..2638)
                     /locus_tag="PKM34_07280"
     CDS             complement(1817..2638)
                     /locus_tag="PKM34_07280"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83428.1"
                     /translation="MNNIFLRIITFVIFVSSVGLTEPVSAQDQPGLMMRTARDYSSQI
                     TLNYQDTLLLNVNQEFDLPVIIKKQSKISAISLGFFYDPVYLEIDSMILAKGTSGFFF
                     SVNDSAFQMAWSSIQPLELEDGDTLLVLNMKTLDISDLTNTLRISLNETSELADTAAN
                     PIEDVVLEIPEIGYLIPDHHDSIGESTICIYPNPFRDHATVFFTLKVGSEVKITVNNS
                     SGIEIFTLTDRPYEPGNHEIRLDKLRMPRGIYLLKFEIRNSDNSGQKVFKIISAF"
     gene            2825..>4465
                     /locus_tag="PKM34_07285"
     CDS             2825..>4465
                     /locus_tag="PKM34_07285"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNQ83429.1"
                     /translation="MKRTILHLLLLAAMLAIPGLSAGQDFLPVLNDNYIGVNQAFLQP
                     ASIADSRFKSDFNAGGFSSDIVSTGIRFKSRWLLDPTGILTNADWWDENTYLDKANGK
                     DKSMFMSQSAIGPSLMVSMGSRHAFALTSRVRSITNVDGMDEPLFRMIYSNFSDKESL
                     YWNRWYYDDNMRAVQHIFGDYGLTYARVFYDQREHFIKAGISLKLLQGIASSYLQTDQ
                     LYYYYDGAEPSGAKPISWNSPYVQGGLSDNWGYWRPDGSYAFDMNFQLTARPSFGMDF
                     GAVYEYRPRWMEHNNPKTGKPRADRNKYFIKVGISVVDLGRLKYVKDYHSSDLVTSFT
                     PDYVNRYNSSDNSVPVNTSWLNAGSVDFTFRKYVPFADTMYRRYSSGQDLGVVRAKDN
                     KEEFKVSLPTAFSIQADVYLIEGVYVSLTTYTALNQGYSKPAHNHYLTTVSVTPRYEQ
                     KWYGFALPIQVDQYGHFEAGLGVRAGIVYFGVSNLFSTVFEDPYGMKGYVGIKIPVPH
                     KKDVVFLPEKEKPFEILLQTEPEEKEKEDKGRIVIIQVS"
BASE COUNT         1227 a         1031 c         1055 g         1152 t
ORIGIN      
        1 gctattattc tactgaagac ctgaaaaatt tccgtcaatg gaaaagcatc acgccgggac
       61 atccggaact cgatgtgctg cacggcatcg agaacacttc cggcccgctc ggacaaggcc
      121 atgcgatggc ggtcggctcg gccatagcgg aacggttcct ggctgcccgg tttggcgagt
      181 ggatggctca caagacattt gccttcatct ccgacggagg tatccaggaa gagatctccc
      241 agggagccgg tcgcatagcc ggttatctcg ggctgaacaa cctggtcatg ttttatgatg
      301 caaataatgt acagttatct acaaaaattg gcgaagttac cagcgaagat accgctatga
      361 aataccgctc ctggaattgg tatgttcaga ccatcaacgg taacaatgca atggagatca
      421 gggaggctat ttcaaatgcc ctggcagaag aacagcgtcc cagcctgatc atcggcaaaa
      481 ccatcatggg cctcggggct gttgattctc aaggtaatag aatggagggc ctcacatcca
      541 cgcacgggca acctttatcc aaagcgggtg tttccttgtc tgatacaatc cggaagctgg
      601 gcggagatcc tgataatcca tttgaagtat cagatgaagt aaaagagtat tatagattaa
      661 tattaagcaa gaaacgtatt tacaccaaag acagagatga tcaaatgaca gcctggaaag
      721 atgataatcc agaccaggca ttattgtgga ataattacac ttcgggggaa gtaccggata
      781 tagatacgga ggctattccc ataaagccgg atgccccaac cagaaatact tcgggcgacg
      841 tgttgtcata tttttcaaaa agtattggaa atatgatcgt tatgtcggcc gatttatcca
      901 acagcgataa aaccgaaggg ttcctgaaac attccaggcc ctttcaaccc ggcgatctta
      961 atggcggatt tctgcatgcg ggtgtatcag agctgaccat ggccgcaatt gccaacggta
     1021 tcgcccttca tggcggtctt tttgttgtct gcggcacctt cttcgtattt tccgattata
     1081 tgaaaccggc tatccggctc gctgccctga tgcggttgcc ggtaaagtat gtttttacgc
     1141 acgactcttt tcgtgtagga gaagatggcc ctacgcatca accggtggaa catgaaatgc
     1201 aactgcgttt gctcgaacaa cttaagaacc accggggtca caacagccta ttggtattac
     1261 ggccggccga cagcgcagaa accatcattt cctggaaact ggccctggaa aataccgaaa
     1321 caccgacagc cctgatcctg tcacgccaga atatcaaaga cataccggct ccggcgggag
     1381 gcaacaggct caaggaggcc gttcataccc gcaaaggcgc ttatactgtt tatgaacctg
     1441 ataatgaacc ggatgtgatt ctactggcca acggttcgga agtttcaact ctgattgagt
     1501 gtgcttttct cctgaagaat gatcacaata ttcaggcccg tgtggtgtca gcgccatcag
     1561 agggactttt tcaacttcag gatgaacatt accgcaagtc aatcattccg gataataccc
     1621 ctgtcttcgg gttaacagcc ggtttaccgg ttacgttaaa gaacctggcg ggttgtaagg
     1681 gaaaagtatt cggactggaa catttcgggt attcggcgcc attccaggta ctggatgaaa
     1741 agtttgggtt taatgctcag aatatcaaag ctatggtact tgattttctt caggaaccta
     1801 tttagatctt ttaaggttaa aaggctgaaa taattttaaa caccttctgc cctgaattat
     1861 ccgaatttct aatttcaaat ttcagtaaat agatccctct gggcatcctc agtttatcta
     1921 atctaatctc atgatttccg ggttcatatg gccggtcagt taaagtgaaa atctcaattc
     1981 ctgatgagtt attcactgtg attttcactt cagaacctac tttcaaagta aaaaatactg
     2041 ttgcatgatc ccggaatgga ttcggataaa tgcaaattgt tgattcacct atagagtcgt
     2101 gatgatcagg aattaagtaa ccaatctcgg gaatctccag aacaacatcc tcaatcggat
     2161 tggctgccgt atccgccagt tcactggttt cattcaagct tatccttaaa gtgttagtca
     2221 aatcagatat atctaacgtc ttcatgttca atactaagag cgtatcccca tcctccaatt
     2281 caaggggctg gatgctggac caggccattt ggaatgcgct gtcgttgacg ctgaaaaaga
     2341 atccggatgt acccttggcc aaaatcatac tgtcaatttc caggtaaacc ggatcgtaaa
     2401 aaaagcctag ggagatggca gatattttac tttgtttctt gatgatcacc ggaagatcaa
     2461 attcttgatt cacgtttaaa agcagggtat cctggtagtt tagggttatc tgtgaactat
     2521 aatccctggc ggttctcatc attaggcccg gctgatcctg ggcagatacc ggttcggtta
     2581 atccaacaga agagacaaaa atgacgaaag taattatcct caaaaaaata ttattcatcc
     2641 aattgaagat caatagtgta acggttttta atgtaccggg taaagttaat gtattttcaa
     2701 cctgcatcga atcaaatgaa aaaaaagagg ccggttcctg aaaccggcct gcagtaaatg
     2761 agtataacac tagagcagtc attgatttat aatgcgtatt aaaaatcggt gatgcagttt
     2821 taagttgaaa agaactatcc tacatctgtt attgcttgcg gccatgctgg ctattccggg
     2881 tttgtcggcc gggcaggact ttctgcctgt gctgaacgac aattatatag gggttaacca
     2941 ggcatttttg cagcccgcct ccattgccga cagccgtttc aaatccgatt tcaacgccgg
     3001 aggtttcagc agcgacatcg tcagcaccgg tatccgcttc aaatcgaggt ggttacttga
     3061 tcctacaggc atacttacca acgctgactg gtgggatgaa aatacttatc ttgacaaagc
     3121 caacggcaaa gacaagagca tgttcatgtc gcaatcggct attggtccca gccttatggt
     3181 ctccatgggc agcaggcacg cctttgccct cacctcccga gtccgcagta tcaccaatgt
     3241 cgacggcatg gatgagcccc tattccggat gatatacagc aatttttcgg acaaagagag
     3301 tctttactgg aaccgttggt attatgatga taacatgagg gcggtgcagc acattttcgg
     3361 ggattacgga ctcacctacg cccgggtatt ctacgaccag cgggagcatt ttatcaaagc
     3421 cggtatttcg ctcaaactgc ttcagggcat tgccagttct tacctgcaaa ccgatcagct
     3481 ttattattac tacgatggtg ctgaaccttc cggcgccaaa cccatttcct ggaattcacc
     3541 ctatgttcag ggcggattat ccgacaactg gggatactgg aggccggatg gcagttatgc
     3601 tttcgatatg aatttccagt taactgccag accctcgttc ggaatggatt tcggcgcggt
     3661 gtatgagtac cgtccgcgct ggatggagca caacaatccg aagaccggca agccgcgggc
     3721 tgaccggaac aagtatttca taaaggttgg catttcggtg gttgacctgg gccggttgaa
     3781 atacgtgaaa gattaccaca gttccgacct ggtgacctca ttcacacccg attacgtaaa
     3841 ccgctacaat tcaagcgata acagcgtacc ggtgaatacc tcctggctca atgccggctc
     3901 ggtggatttc accttcagga agtacgttcc gtttgccgac acgatgtacc ggcggtactc
     3961 atccggacag gatctgggag ttgtcagggc aaaagataac aaagaagagt tcaaagtcag
     4021 tctgccgacc gctttcagta tccaggccga cgtttacctg atcgaaggcg tatatgtcag
     4081 tctcacaacc tataccgctt taaatcaggg atatagcaag cctgcgcaca atcattacct
     4141 gacaacggtc tcggtcactc cacggtatga gcaaaagtgg tatggttttg ccctacccat
     4201 ccaggttgac cagtacggac attttgaagc aggattggga gtcagggcgg gtatcgttta
     4261 cttcggcgtc agcaacctgt tttcgaccgt ttttgaagat ccatacggaa tgaaaggata
     4321 cgtagggatt aagattccgg tgccgcataa aaaagatgta gtcttcctcc cggaaaaaga
     4381 gaaacccttt gaaattcttc tgcaaacaga acctgaagaa aaagaaaagg aggataaagg
     4441 ccggattgtc attattcagg tcagt
//
LOCUS       DAOQXS010000233         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19912_length_4461_cov_4.064911, whole genome shotgun sequence.
ACCESSION   DAOQXS010000233 DAOQXS010000000
VERSION     DAOQXS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19912_length_4461_cov_4.064911"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..665
                     /locus_tag="PKM34_07290"
     CDS             54..665
                     /locus_tag="PKM34_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ83430.1"
                     /translation="MMESSERIILKSIELFTRYGIRLVTMDQIAEEVGMSKRTIYELF
                     KDKDTLVMECLESIHRKQMTEIQEIAERVSNVIEALYRVGQYSEKKKAEMNPIFFEDI
                     RKLYPQIWNTMKQRSNTGSGMFSHMILTKGIGEGIFRDDLDITIVGTFMQSMMETFHK
                     KEIFPENTPDKALLRNIVIPYYMGISTDKGKKLIEQYIPLTFS"
     gene            691..1989
                     /locus_tag="PKM34_07295"
     CDS             691..1989
                     /locus_tag="PKM34_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ83431.1"
                     /translation="MRKTALFLLLWAFTGCFQASGQEKLALSLEQAINYALEYNKQLE
                     NAAISVDLAQKKINESISGFLPQADLSMDYSNYLGAEMEFSFGEGLPAQKIPFNPTSN
                     LYLRVSQMVFSGNMIVGLQMVKIYKEMSSTNYLKTEQSLKENTIKAYQNALVSEKYIG
                     IINENLANTRQIYENTKAMVSVGMAEKLDLDQFEVTLSTLENALKSAERQKELAYNLL
                     RFQLGVQADTPVDLTDSLGFFIENFDITGLADKSFNLSNNLDYQVMQSQVQISGKQVS
                     MQKMNYLPTLTGFYQHTNKLMKPEFDLSPKNVIGLNFNFPILSSGMRNSQYNQARLQY
                     ETTLNNKSLLTDQLLLQEKQLRFNLASAIEQYLNRKNNVDVTYRIYKSYRFKYEQGIA
                     SSLDLTIANNNYLQAESDYLMSIMTLLDARTALQKLLNTL"
     gene            2014..3078
                     /locus_tag="PKM34_07300"
     CDS             2014..3078
                     /locus_tag="PKM34_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ83432.1"
                     /translation="MKFLRSLPAIVLSVFILAGCGSMEGEKKNESSAASASAIKIEKV
                     KVMALEPVTIGKNIEYTSTLKAFEEVHLVPSTPGRIEKIYVEVGSRVSKGDVLVQMDQ
                     IQLRQAIIQLQTLETDYKRLDTLQKVGSITRQQFDQISAQVEIARNNVEFLKENTRLV
                     APFGGIISGKYFEDGEMYSGAPNTTAGKAAILSIVQINPLKAIVNIPETYFPAVGEGM
                     KTRVLSDLYRDQEYAGQIMRKYPTIDPNTHSFQAEIRIENPGEKLRPGMFCRVELGFG
                     EIEALVVPALAVLKMQGSNERYIFLADQGKAKRVSVTIGQRFDDKVEIISNELKVGDQ
                     LIIAGQSRLVDQVPVEIVIE"
     gene            3121..>4461
                     /locus_tag="PKM34_07305"
     CDS             3121..>4461
                     /locus_tag="PKM34_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ83433.1"
                     /translation="MSIYKSAVNKPITTVMIFTAVVVLGLYSLINIPIDLYPEIEPPY
                     ISIMTTYAGANAADIETNITKPIEDALNSVDKLKEVTSVSYDNLSLVSMEFEWGSNLD
                     EVLNDVRSVIDGVYNYLPDGCDRPSIFKFSTSMMPILFYAITAEESYPGLEKIIEEKL
                     VNPLNRIEGIGSITTMGSPKRIIYIDVDPRKLDAYALSLEQIGNTIAAENLNMPSGNV
                     KMGKFDYQLRVEGEFEESDHIREIIVGNIGGKAVYLKDIATVRDTLKYGFIDEKINGK
                     QGMRLLVMKQSGANTVSIARDVHAKMDELVKTLPPDISVNPIFDSSDFIKGSIDNLSE
                     TLMWAFLFVILIVLFFLGRWRATLIIILTIPISLVVSFIYLYFTGSSLNVISLSSLSI
                     AIGMVVDDAIVVLENITKHIERGSSPREAAIYTTNEVWLAVIITTLVIVAVFLPL"
BASE COUNT         1294 a         1122 c         1017 g         1028 t
ORIGIN      
        1 atgaaaactg aataaaccga tcaagtttta ctatttttgc cgctcatttt gttatgatgg
       61 aatcgagcga aagaattatc ctgaaatcca ttgaactgtt tacccgatac ggcatccggc
      121 tggtaaccat ggaccagatc gccgaagagg tgggtatgtc gaaacggaca atctatgaat
      181 tgtttaaaga caaagacacc ctggtcatgg agtgcctgga atcgatccac cggaaacaaa
      241 tgaccgaaat acaggaaatt gccgaaagag tttcaaacgt catcgaggct ttataccgcg
      301 tcgggcaata tagcgagaaa aagaaagcag aaatgaatcc catttttttt gaagatatcc
      361 ggaaactata tcctcaaata tggaatacca tgaaacaacg ctccaatacc gggtccggta
      421 tgttctccca tatgatcctg acaaaaggga ttggggaagg cattttccgg gatgacctgg
      481 atattacaat tgtcggtaca ttcatgcaat ccatgatgga gacttttcac aaaaaagaga
      541 tattccccga aaacacccct gacaaagccc tgctccgcaa tatagtcatc ccttactata
      601 tgggtatctc aacggataaa gggaagaaac tcatcgaaca atatatacca ttaacatttt
      661 cttgatcact actaataatt atattcagca atgcgtaaaa cagcgttatt tcttttatta
      721 tgggcgttta caggctgttt tcaggcatca ggacaggaaa aactggcact atccctggaa
      781 caggcaataa attatgcact cgaatacaac aagcaactcg agaatgccgc tatctccgtc
      841 gacctggccc agaaaaaaat aaacgaatcg atttccggtt tcctccccca ggccgatctg
      901 tctatggatt actcgaacta tctcggcgcc gaaatggagt tcagcttcgg ggaaggcctc
      961 ccggcacaga aaattccgtt taaccctacc agcaacctgt atctcagggt atcccaaatg
     1021 gtcttcagcg gaaatatgat cgtcgggctt caaatggtga agatctataa ggagatgtcg
     1081 tctacaaatt acctgaaaac ggaacaaagc ctgaaggaaa acaccatcaa agcctaccag
     1141 aatgccctgg tatcggaaaa atatatcggc attatcaatg aaaacctggc caatacccgc
     1201 cagatctacg aaaacaccaa agcgatggtc tcggtgggta tggcggaaaa actcgatctc
     1261 gaccagttcg aggtcaccct ttccacactg gaaaatgccc tgaaatcggc cgaaaggcag
     1321 aaagaactgg cctacaacct gctccgcttc cagctgggcg tgcaggccga tactcccgtc
     1381 gatctgacag attctcttgg cttcttcatc gaaaacttcg atatcacagg actggccgac
     1441 aaatcgttca atttgtcgaa taaccttgat taccaggtca tgcagagcca ggtacagatc
     1501 tccggaaagc aggtcagtat gcaaaagatg aattacctgc cgacactgac cgggttttac
     1561 cagcatacca acaagctgat gaaaccggaa ttcgatttgt ctccgaaaaa tgtaatcggc
     1621 ctgaacttta atttcccgat cctgtcgagc ggtatgcgca attcgcagta taaccaggcc
     1681 cgcctccagt acgagacaac ccttaacaac aaaagcctgc tgaccgacca gctgctcctc
     1741 caggaaaaac aattgcggtt caacctggcc agcgccatcg aacagtatct caaccggaag
     1801 aacaatgtgg atgttacata ccgcatctat aaaagctacc ggttcaagta cgagcaggga
     1861 atagcctcaa gccttgacct gacgatcgcc aataacaatt atctgcaggc cgaatcggat
     1921 tacctcatgt cgatcatgac cttgctcgac gcacgtaccg ccctgcaaaa acttttgaat
     1981 acactataat cgtaacaata aaaatcaagt tttatgaaat ttttaagatc acttccggca
     2041 attgtactat ccgtattcat ccttgcagga tgcggatcca tggaaggaga gaaaaaaaat
     2101 gaatcatcgg ccgcttcagc ctccgctatt aaaatagaga aagttaaggt tatggcgctg
     2161 gaaccggtaa cgatcgggaa aaacatcgaa tatacctcaa cgctgaaagc cttcgaagag
     2221 gtgcacctgg tgccttccac cccgggccgg attgagaaga tttatgtcga agtcggaagc
     2281 cgtgtcagta aaggggatgt gctggtgcaa atggaccaga tccaacttcg gcaggcaatc
     2341 atccaattgc agacactgga aaccgattac aaacggctcg atacactgca gaaagtaggc
     2401 agtattaccc gccagcaatt cgaccagatc agcgcccagg tggagattgc ccgcaacaat
     2461 gtggaatttc tcaaggaaaa tacccggctc gtagctcctt tcgggggcat tatctccggt
     2521 aaatatttcg aagatggcga aatgtattcg ggtgcgccga acaccactgc gggcaaagcg
     2581 gccatccttt cgatcgtgca gatcaatccg ctcaaggcca tcgtcaatat ccccgagact
     2641 tacttcccgg ctgtcggcga agggatgaaa actcgcgtgc tcagcgacct ttaccgcgac
     2701 caggaatatg ccggccagat catgcgcaaa tacccgacca tcgatccgaa tacacattcc
     2761 ttccaggccg agatcaggat cgaaaacccg ggagaaaaac tccgcccggg aatgttctgc
     2821 cgcgtcgaac tgggcttcgg cgagatcgaa gcgctggtag tccccgccct ggccgtcctt
     2881 aaaatgcagg gatccaacga gcgttacatc ttcctggccg accagggtaa agccaaaagg
     2941 gtatccgtga cgatcggcca gcgcttcgac gacaaagtgg agatcatctc caatgagctg
     3001 aaagtcgggg atcagctcat catcgccggc cagtcgaggc tggtcgacca ggtaccggtc
     3061 gaaatcgtca tcgaataatc atttaaaaag taatccgatt aactttttaa gatcaataca
     3121 atgagtattt ataaaagcgc tgtcaacaaa cccattacca ccgtcatgat attcacggcg
     3181 gtcgtagtgc tcgggttata ctcgctcatt aatataccga tcgatctgta tcccgagatc
     3241 gaaccgccct atatcagcat catgaccacc tacgcagggg ccaatgccgc ggatatcgaa
     3301 accaacatca ccaagccgat cgaagacgcg ctcaactccg tcgataagct gaaagaggtg
     3361 acttcggttt cttatgataa cctctcactg gtcagtatgg aatttgaatg gggttccaac
     3421 ctcgatgaag tacttaacga tgtaagatcg gtgatcgatg gcgtttacaa ttatctgccc
     3481 gacgggtgcg acaggccgtc catcttcaag ttcagtacca gtatgatgcc gatcctgttc
     3541 tatgccatta cggctgagga aagctatccc ggcctcgaaa agatcatcga ggaaaaactg
     3601 gtcaatccgc ttaacaggat cgaagggatc ggctccatca ccacgatggg ttcgccaaaa
     3661 cgtatcattt acatcgatgt cgatccccgg aaactggatg cttatgccct gagcctcgaa
     3721 cagatcggta acaccatcgc agccgagaac ctgaatatgc cttcagggaa tgtcaagatg
     3781 ggaaaattcg attaccagct cagggtggaa ggtgagtttg aagaaagcga tcatatcagg
     3841 gaaatcatcg tcggtaatat cggcgggaaa gcggtttacc tgaaagatat cgctactgtc
     3901 agggatacac tcaagtatgg ttttatcgat gagaagatca atggtaaaca gggaatgcgc
     3961 ctcctggtca tgaaacaatc gggagccaat acagtaagta tcgcccggga tgttcatgcg
     4021 aaaatggatg agttggtaaa aactttgccg cccgatatca gtgtaaaccc gatattcgac
     4081 agctccgact ttatcaaagg atccatcgac aacctgtcgg aaaccctgat gtgggctttt
     4141 cttttcgtta tcctgatcgt cttgttcttc ctgggccgct ggagggcaac attgatcatt
     4201 atcctgacca tcccgatctc gctggtggtc tcctttatct atctctactt taccggcagt
     4261 tcgctgaacg tgatctcact ctcctcgctg tcgatcgcga tcggaatggt ggtcgatgat
     4321 gcgatcgtgg tcctcgaaaa catcaccaag catatcgaaa ggggatccag cccgcgggaa
     4381 gcagccattt ataccaccaa cgaagtgtgg ctggccgtca ttatcactac ccttgtcatc
     4441 gttgccgtat tccttcccct g
//
LOCUS       DAOQXS010000234         4460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19922_length_4460_cov_3.496708, whole genome shotgun sequence.
ACCESSION   DAOQXS010000234 DAOQXS010000000
VERSION     DAOQXS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19922_length_4460_cov_3.496708"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1736)
                     /locus_tag="PKM34_07310"
     CDS             complement(282..1736)
                     /locus_tag="PKM34_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83434.1"
                     /translation="MKKFYLLCLLLILFMITWAQSYIPLPDTNAYWNEIKFYQGQCDP
                     PDFCKYTCYFQGDTILNSLSYHKVYKNDSYEISYSGGLRQDGEKVYYWDRDCNSPVLL
                     YDFGLTVGDSIALACMLCDEEPLYAQVVSVDSVMIGDMSYRKRINFTYGYSYSWIEGI
                     GSVYGLLYPYYSCILCVCYQKLVCFEQDGQVLYLNESDVPCFNFVNPPGDIDPASEWR
                     VDSWTAEPDYSTNSYYRDFIDGDTIINTIRYHKVYQSGYTYYLNYPPGYYYYYEDVFH
                     GLLREEDNRWYTINYNGQDELLYDFTLDVGDTVYSAFTFIFEDPIIVTGVDSVLVGGD
                     YKRRLELNSEEGGGAEYIIEDIGATSGLFENMFFFEWGSELVCFAKNGVSLWGASTEE
                     CDLAVAIGEAETHPNACFISPNPATDHLILTLYGSIGEASFTLINLLGNVVMEGSFTS
                     PSIIKIDLNACSPGIYLAVIKTADTRETFKVVKR"
     gene            complement(1773..2438)
                     /locus_tag="PKM34_07315"
     CDS             complement(1773..2438)
                     /locus_tag="PKM34_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83435.1"
                     /translation="MKKTVFTLLLLGIVPFTLQSQFLKGTILKTKPFQDFVAYNPTFG
                     MEKTVGKLFSAELELMYRNRSWNVSGGEWDLGRFYDAGGFKIVIGSRIYFGKTNKNLD
                     QETQKAPFAWYGAIQLGYNLSTTYNVDRKATVSGIYMYTADIDKNWLELNIGIGRQFY
                     LFKTITLDLNLGPSIYFPHQEKTTITKSEDLSEIGTTKTEDFNFGRIIRPNLILSLGY
                     YFK"
     gene            complement(2573..>4460)
                     /locus_tag="PKM34_07320"
     CDS             complement(2573..>4460)
                     /locus_tag="PKM34_07320"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNQ83436.1"
                     /translation="NYGWAVGNGATILHTKNGGNDWMIQHGGSGSGSYINSVYFSDSL
                     HGWTDGTNGGGQYFETTDGGNTWLWRSIPGVSYSWELSSMFFTDSLFFWALTGVDQIS
                     HTEDGGNTWITGPSPTGWGEMFFVNHDTGWMTDSHRIYRTTDRALTWEEQLPMQGPAI
                     CDLYFTDALHGWAGGEGLLLKTADGGESWESYETDVLFLAIEFSDMLNGWAVGGWAGE
                     LYHTLDGGNTWEDKRAGSDGYITAISFGSPDFGLASYYEDDYHSLLMTTDGGAGWDRL
                     TILEDSIRWNALWVEDDINAWACGDDGTIVHSDDRGQSWVVQREEVPGDWFSELQFLD
                     PLTGFSVGIDAGYTNILLARTYDGGQNWFTLPFHYSASPQGMWFTDPLKGFLVATDMN
                     TDAQVLLTTYSGGESWIITDLPLDDDEDASSVCFTTSLKGFIGADNCILRTYDGGQNW
                     ERYNINGGQIFSLSFPDSLNGWCIGKIYSDRAVLRTNNGGESWAEVPVISDNTLTDLA
                     FTAPDNGWICGYDGLILKWGENNPVSIRDDDTPVYVSVPLISPNPASHWLNIDLTVFP
                     ENQKISYRIFNIQGEEIGAGSYQKKGSHDRLTIDIKAINNGIYIVELAMGGERWAGRF
                     VVIK"
BASE COUNT         1215 a         1085 c          951 g         1209 t
ORIGIN      
        1 attttccagg ttgaatgatg caatcttgat gggtgtcatt ggattgtttt ttaaggatta
       61 attgttaaaa aagttgattt atccttatgc aaaggattga gggtcaaata taagcaaaaa
      121 tataggaaaa gtctagatat ttatgtagat ttttttaatt aatgagactc catctcaatt
      181 tttcgagatg gagtctcgat gaaaggagat ggagtctcac cgaaatgaga tggcgtctcg
      241 atcacctaca atccatcttg ctcgaagcat aaaagaggca actatctttt aaccaccttg
      301 aaggtttctc ttgtatctgc tgttttgata accgctaagt agatacccgg agaacaagca
      361 tttaaatcaa ttttaataat ggatggagaa gtaaatgagc cctccatgac cacatttccc
      421 aaaagattga tcagggtgaa agatgcttct ccgatgcttc cgtataatgt caggatcaaa
      481 tggtcagttg ccgggttggg tgaaataaag cacgcgttcg gatgggtttc tgcttccccg
      541 atagccacgg ccaggtcgca ctcttcggtt gaagctcccc acagagaaac gccgttctta
      601 gcaaagcaaa ccagttccga tccccattcg aagaagaaca tgttttcgaa caggccggaa
      661 gtggccccaa tatcctctat aatgtattcc gctccgccgc cttcttcaga attcaattcc
      721 aggcgccttt tgtaatctcc gccaaccagg accgaatcga cgcccgttac gatgatcgga
      781 tcttcaaaaa taaaagtaaa cgccgagtaa acagtatcgc ctacatccag ggtaaaatcg
      841 tacagcaact catcctggcc attgtaatta attgtatacc agcggttatc ttcttccctc
      901 aacagcccat gaaatacatc ctcataataa taataatatc ccggaggata attcagataa
      961 taggtatatc ctgattggta aaccttatga tatcttattg tattgatgat ggtatcccca
     1021 tctataaaat cccggtagta tgaattcgtc gaataatccg gttcggccgt ccacgaatcc
     1081 acgcgccatt cggaggccgg atcgatgtct cccggtggat tgacgaaatt gaaacaggga
     1141 acatccgatt cattcaggta aaggacctgg ccatcctgct cgaagcagac cagtttctgg
     1201 taacagacac ataaaatgca ggaataatac ggatacagca agccgtacac gcttccgatc
     1261 ccttcgatcc acgaatagga ataaccatag gtgaaattga tccttttccg gtagctcata
     1321 tccccgatca tcaccgagtc gacggagact acctgggcat acagcggttc ctcatcgcaa
     1381 agcatgcagg ccagggctat ggaatccccg acggtcagcc cgaaatcata taaaagcacc
     1441 ggagaattgc agtcccgatc ccagtaatag actttttcac catcctgcct taatcctccg
     1501 gaataggaaa tttcataact gtcattttta taaactttat gataagataa agaattgaga
     1561 attgtatccc cctggaaata gcaggtgtac ttgcaaaaat ccggcgggtc gcactggccc
     1621 tggtagaatt ttatctcatt ccaataagca ttggtatccg gtaaagggat atacgattgt
     1681 gcccaggtaa tcatgaacag tatgagaagc aggcataata gataaaactt tttcatggct
     1741 attttgtttt gatgcgggat tggccgctgt tattatttaa aataatatcc taatgatagg
     1801 attagatttg gcctgataat tcgtccaaaa ttgaaatcct cggttttcgt cgtcccgatt
     1861 tcgctcaaat cttcactttt tgtaatggtt gttttttcct ggtgtggaaa ataaatggag
     1921 ggccccaggt taagatccag ggttattgtt ttgaataagt aaaattgccg tccgataccg
     1981 atattcagct ccagccagtt tttatctatg tcagccgtat acatataaat gcctgaaact
     2041 gtagctttcc ggtcgacatt atacgtagtg ctgaggttat agcccaactg gatggctcca
     2101 taccaggcaa acggagcttt ttgagtttct tgatccaggt tcttgtttgt ttttccaaaa
     2161 tagatccttg atccgatcac aattttgaat ccgccggcat cgtaaaagcg gccaagatcc
     2221 cattctccac cggatacatt ccagctcctg ttacggtaca taagttcaag ctctgcgctg
     2281 aataacttac caaccgtttt ttccattcca aaggtcggat tataagcgac gaaatcctga
     2341 aagggttttg ttttaaggat tgtccctttt aagaattgag attgtaaggt aaatggaact
     2401 attcccagca acagtagtgt gaatacggtc tttttcataa tctacttata aagctatata
     2461 tcaatcattt gttccgcctg gcatatccgt gcactgtgag actccatctc aatttttcga
     2521 gatggagtct cgagtgaaag cagatggcat ctcaccggtt cgagatggag tctcatttta
     2581 taacaacaaa cctcccagcc catctttctc cgcccatggc cagttcgaca atatatattc
     2641 cattattaat tgctttaata tcaatcgtta accggtcatg gctccctttt ttttgatatg
     2701 atcccgcccc gatctcctca ccctgaatat taaagattct atatgatatt ttctggtttt
     2761 ccgggaaaac ggtaaggtca atgttcagcc aatggcttgc cggattcggg gaaattagcg
     2821 gaacggaaac ataaacaggc gtatcatcat ccctgataga gaccgggttg ttttcgcccc
     2881 atttcaggat caggccatcg tatccgcaga tccatccgtt gtcaggcgct gtgaaagcta
     2941 agtcggtcaa ggtattgtcg gaaatcacag ggacctcagc ccagctttca ccgccattgt
     3001 ttgtgcgcaa tactgctcta tcagaataaa ttttgccaat acaccagccg ttcagggaat
     3061 cgggaaagga aagggaaaat atctggcctc cattgatatt gtatctctcc cagttctggc
     3121 ctccgtcgta ggttcgcaga atgcaattgt cggctccgat aaagcctttt aatgaagtgg
     3181 taaagcaaac cgacgaagca tcctcatcat catcgagcgg caggtcggtg atgatccagc
     3241 tttcgccacc ggaataggtt gtcaggagaa cctgtgcgtc cgtgttcatg tcggtggcta
     3301 caagaaatcc ctttaacgga tcggtaaacc acataccctg tggcgaagcc gaataatgga
     3361 acggtagagt aaaccagttc tgccctccgt cgtaagtcct ggcaagaagg atattggtgt
     3421 acccggcatc aatccctaca gaaaatccgg tcaggggatc caggaactgc agttcagaaa
     3481 accagtctcc aggcacctct tcccgctgca caacccagga ttgcccacga tcgtccgaat
     3541 gaacaatggt tccgtcgtcg ccgcaggccc aggcattgat atcatcctcc acccaaagag
     3601 cattccaacg tatggaatct tccaaaatcg tcaggcggtc ccatccggcc ccaccgtctg
     3661 ttgtcatcaa cagggagtgg taatcatctt cgtaatagct tgccaaacca aagtcgggac
     3721 tcccgaaact gatggcagtg atatacccat cactgcctgc acgcttatct tcccatgtat
     3781 ttcctccgtc aagtgtatga tataattctc ctgcccaacc tcctacggcc catccgttca
     3841 gcatatctga aaactcaata gccaggaaga gcacatctgt ttcgtaactc tcccaggatt
     3901 ctccgccatc cgctgttttt agtaataagc cctcaccacc tgcccatcca tgcagggcgt
     3961 cagtaaaata gagatcgcaa atagccggcc cctgcatcgg caactgttct tcccaggtca
     4021 gggctctgtc ggtggtgcgg taaatccggt gactgtcggt catccatcct gtatcatgat
     4081 ttacgaaaaa catctctccc cagccggttg gcgatggccc cgttatccag gtatttccgc
     4141 cgtcttccgt gtgcgaaatc tggtcgactc ccgttagggc ccagaagaaa agggaatcgg
     4201 taaaaaacat ggacgaaagt tcccaggaat acgaaacgcc cggaatgctt ctccacagcc
     4261 aggtattacc cccatcggtt gtttcaaaat actggcctcc accgttggtt ccatcagtcc
     4321 atccgtgtaa tgagtcagaa aaatagaccg aattgatata agaaccggag ccggaaccgc
     4381 cgtgctggat catccaatcg tttccgccat tcttggtatg caggatcgtg gcgccgtttc
     4441 cgactgccca gccatagttt
//
LOCUS       DAOQXS010000235         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_19959_length_4455_cov_3.800909, whole genome shotgun sequence.
ACCESSION   DAOQXS010000235 DAOQXS010000000
VERSION     DAOQXS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19959_length_4455_cov_3.800909"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKM34_07325"
     CDS             complement(<1..414)
                     /locus_tag="PKM34_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNQ83437.1"
                     /translation="MNTIDQIIQTRVLLLDGAMGTMIQRYRLTEEDYRGERFKDCGRD
                     LKGNNDLLSITRPQIIREIHNAYLEAGADIIETNTFNANAISQADYGMESLAYELNLA
                     SASLARQCADAFTLKDPSRPRFVAGALGPTNKTASL"
     gene            complement(899..2695)
                     /locus_tag="PKM34_07330"
     CDS             complement(899..2695)
                     /locus_tag="PKM34_07330"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83438.1"
                     /translation="MRFAFFILHLPGFRIFIPAILAFIVFLTDDLAGQSICEKATQGV
                     LNDAGHQASRWYFGEYAGLDFRQGNPVVDLTNDWVNAITSPAIISDSAGEIVFFTNGI
                     RVFNRLGDTMPNGSGLHAFTGHPMPVLIVPWPGRDSLYYVITTGRIKQNENDQSTIYG
                     LEYHVVNMNLDSGYGDIDLKNKVLLEPEVSPKVCAVKHSNGTGYWLVAHRFNSNEFCS
                     FLITEEGIDTAGYVSSFIGTPHLGPGETNNGIGYMKISPDGTKIALAIFGSDICEVFD
                     FDASTGVVSNVITSEPVFDELFGVEFSPDSRFLYLTITSVSLPTPAYTPPSYLFQMDM
                     NAGTSIFEPGSFDTIAFDTTGSYFGGIQLGPDGRIYVSRSPYGNSVISVIQNPKRKGS
                     ACNFISNAIDLEGRHSRFGFPNFIQSWLDLPHFSANSTGNNFEYAFTLQDDSNIDEIL
                     WDFGDPGSSQNTSTDFQPLHVFSSEGTYEVSVTEFAGRTAYGPYTETIVIPSTSGIDD
                     AVDFSGFCKVYPNPGNGDLRIVFDESQKRVMISVKNSLGHHLLGPVSYNNLAEDSEIY
                     LDISELGKGLFFLVFQNERGDHYTIKYLLREE"
     gene            complement(2708..2784)
                     /locus_tag="PKM34_07335"
     tRNA            complement(2708..2784)
                     /locus_tag="PKM34_07335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2748..2750),aa:Ala,seq:cgc)
     gene            complement(2872..4149)
                     /locus_tag="PKM34_07340"
     CDS             complement(2872..4149)
                     /locus_tag="PKM34_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNQ83439.1"
                     /translation="MNTLKKPKSLEILNDDWMAVVVGFIIIALTILIYEFVPSLPDLF
                     KPKDKWSGTDFLTGFLSKPNLLRIVYIFVFFAILAWLGLKFAGKKTTYFLGSFAVIAL
                     LAILAQVISTHTSIKAVSLETVFFSILIGLLISNTVKLPEWLKQSVQSEFYIKIGLVL
                     LGTSVLFTKIMSAGALGLVQALVVVVSVWYIAFWISRKLKIDPEMSTMLASAVSICGV
                     SAAIATCGAIKGDSKKLSYVISLVLIVAIPMMIFMPYLAKWMGLSQEVAGAWIGGTID
                     TTGAVVASGTIIGEIAQKYAVIIKSAQNVLLGLAAFAISIYWSYSQKSPSAEKPTARI
                     IWDRFPKFVIGFILASLLFSFALDAETVKQAGDTIKGYQNIWFSMAFVCIGLETRFRE
                     IFTAENRKPLYAFLIAQTINIIITLIIAYLLFR"
     gene            4227..>4455
                     /locus_tag="PKM34_07345"
     CDS             4227..>4455
                     /locus_tag="PKM34_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83440.1"
                     /translation="MTMFHFTRISMISIILSLLFVFCDNTDKPDGTGDPANLSITVLS
                     VDMETGDVTLQATADNTVRYLLYIDAETNPAD"
BASE COUNT         1218 a         1063 c         1056 g         1118 t
ORIGIN      
        1 gagggaagcg gtcttgtttg tcgggccgag tgcgccggct acgaagcgcg gccgggacgg
       61 atctttcagg gtaaacgcgt cggcgcattg ccgggctaaa ctggctgaag ccaggttaag
      121 ctcataggcc agcgactcca tcccgtagtc ggcctgggat atggcattgg cattgaaagt
      181 attggtttcg atgatatcgg ctcccgcttc gagataggca ttgtggatct cccgtatgat
      241 ctgcggccgg gtgatggata gcaggtcgtt attccctttc aggtcacggc cgcagtcttt
      301 gaaacgttct ccgcggtaat cctcctctgt cagccggtac cgctggatca tcgtccccat
      361 cgccccgtcg agcagcagta cacgcgtttg tattatttga tcaatggtat tcaatggaaa
      421 aaagtcgtta gtcgttaatc gttggtcgct tgtcctgagt ccgccgcagg cgggcgaagg
      481 atcagtcatt tgtttttggt catttattaa aaataacaat cgaacaatag aacaattgag
      541 cctttttgaa ttagtgtgtg gttttatgga tcacgccttc ggcataagca ttgctgaagg
      601 ctcatgccat gcacataggc attgaaaggg agtagtttga tggtgcggtc attttttgcc
      661 ggttttcaga atgcaaaggt aatatttata ttgattctaa tttacggaca cttttccgcg
      721 ctcaggataa ttgtttgatt cgcgatgagg ctgtctccaa agtctatttt taaccacaaa
      781 ggcgcacaaa gtacatcaca aaggcgcaca aagtatcaga ttatcaaata ataaactttg
      841 tgttccttcg tgaaatcctt ggtgttcctt tgtggtttaa actaatgata tagccaattc
      901 attcctcccg gagaagatat tttatggtat aatgatcgcc tctttcattt tgaaatacaa
      961 gaaagaataa tcctttaccc aactcggaaa tatccaaata gatttcagaa tcttcggcta
     1021 aattattata agagacaggt ccaagcagat gatgacccag cgaattcttc acgctgatca
     1081 tcactctttt ctgggattca tcaaaaacaa tcctgagatc tccattgccg ggattcggat
     1141 aaactttgca aaaaccggaa aaatcaacgg catcatctat tccggaagtg ctgggtatga
     1201 cgatcgtttc ggtatacggg ccataagctg tccggcctgc gaactctgtc accgacactt
     1261 cgtaggtccc ttcggaagaa aacacatgta atggttgaaa atccgttgag gtattctggc
     1321 tgcttcccgg atcgccgaaa tcccataaaa tctcatcgat attggaatca tcctgcaggg
     1381 taaaagcata ctcgaaattg ttaccggttg aattcgcgga aaagtgggga agatccaacc
     1441 atgactggat gaaattcgga aaaccgaagc ggctgtgcct gccttccaaa tcgatggcgt
     1501 tggaaataaa gttacaggcg cttccttttc ttttgggatt ttgaataacg ctgataaccg
     1561 agtttccgta aggcgaccgg gaaacataga tcctgccgtc gggacccagc tgaatgcccc
     1621 cgaaataact gcctgtggta tcgaaagcga tagtatcaaa tgatcccggt tcgaaaatgc
     1681 ttgttccggc attcatatcc atttgaaaaa ggtaagaggg gggtgtataa gccggcgtcg
     1741 gtaacgacac tgaggtaatg gtcaggtaaa gaaaccggga atcgggtgaa aactccaccc
     1801 cgaataattc atcaaaaaca ggttcggaag tgatgacatt ggagaccacg ccggtcgacg
     1861 cgtcaaaatc aaacacttcg caaatatccg atccgaaaat cgccagcgcg atttttgtac
     1921 catccgggct gattttcata tacccaatcc cattgttggt ctctcccggc cccaggtgtg
     1981 gggttccgat aaaactggaa acgtagccgg cggtatcaat tccttcttcc gtaatcagaa
     2041 acgagcaaaa ttcgttcgaa ttgaacctgt gagccaccag ccagtaacct gtcccattgc
     2101 tgtgtttcac tgcacaaact ttcggcgaca cctcaggctc aagcaaaacc ttatttttca
     2161 agtcaatatc gccatatccc gaatccaggt tcatatttac aacatgatac tccagaccgt
     2221 aaatagtact ctgatcattc tcattttgtt ttatccttcc ggtggtgatc acatagtaga
     2281 gactgtcacg gcccggccag ggaacaatta aaaccggcat cggatggccg gtaaaagcat
     2341 gcaatcccga cccgttgggc atcgtatccc caaggcggtt aaatactctt atcccattcg
     2401 taaaaaacac gatctcacct gcactgtcgc tgataattgc cgggcttgtt atagcattga
     2461 cccagtcatt ggtcaggtcc accaccgggt tgccctgcct gaaatccagc ccggcatact
     2521 ccccgaagta ccaccgcgag gcctggtgac cagcatcgtt cagaacgcct tgcgtggcct
     2581 tctcgcaaat ggactgaccg gcaagatcat ccgtcaggaa gacaataaac gccaggatag
     2641 ccggaataaa aatccggaaa ccgggtaaat gcaaaatgaa aaatgcaaaa cgcaaaatgg
     2701 aacatgttgg tggagtatac cggagtcgaa ccggtgactt ctgcattgcg aacgcagcgc
     2761 tctggccatc tgagctaata ccccgaaatg ctaattctta atttttaatt tttaatgagt
     2821 aattcaaaat taagaattca aaattaagaa ttaagaatta atcgcctgat attatctgaa
     2881 caataaatat gcaataatta aggtgatgat gatattgatg gtttgtgcga tcaggaaggc
     2941 gtacaggggt ttgcggtttt cagcggtgaa gatttcgcgg aaccgggttt ccaaccctat
     3001 gcacacaaaa gccatactga accatatgtt ctggtaacct ttgatcgtgt cgccggcctg
     3061 tttgaccgtc tcggcgtcga gcgcaaatga gaacaacagg gatgccagga taaaaccgat
     3121 gacgaacttc ggaaagcggt cccagataat tcttgcggtc ggtttttcgg ccgacggcga
     3181 tttttgcgaa tacgaccaat agatcgaaat ggcgaaggcg gcgaggccaa gcagcacatt
     3241 ctgcgccgat ttgatgataa cggcatactt ctgggcgatc tccccgatga tggttcccga
     3301 agcgaccacg gccccggtcg tatcgatcgt cccaccgatc caggcgccgg ccacctcctg
     3361 cgaaagcccc atccatttgg ccaggtaagg catgaagatc atcatcggga tagcgacgat
     3421 caataccagc gaaataacgt aagagagctt tttgctgtcg ccttttatgg cgccgcaggt
     3481 ggcgatggcc gccgacacgc cgcagatcga aacggccgag gccagcatcg tcgacatctc
     3541 cggatcgatc ttcagcttgc gcgaaatcca gaaagcgatg taccagaccg aaacgacaac
     3601 aaccagcgcc tgcaccagcc ccagcgctcc cgccgacatg atcttcgtaa acagcaccga
     3661 agtccccagc aagaccagcc cgatcttgat gtagaattcg ctttgcaccg attgtttgag
     3721 ccattcgggc agcttcaccg tattgctgat cagcaggccg atcagtatgg aaaagaaaac
     3781 ggtctccagg ctcaccgctt tgatcgacgt atgggtcgag atcacctgcg ccaggatggc
     3841 cagcaaagct atgacggcaa acgagcccag gaaataggtg gtcttcttgc cggcaaactt
     3901 cagccctagc caggccagga tagcaaaaaa gacaaaaatg tagacgatcc tcaaaaggtt
     3961 tggcttcgac aggaacccgg tcagaaaatc ggtgccgctc catttgtctt ttggtttgaa
     4021 aagatcgggc aacgagggga caaattcata gatcaggatg gtcagggcaa tgatgataaa
     4081 acccacaacg acagccatcc agtcatcatt gagtatttcc agtgattttg gctttttaag
     4141 ggtattcata cttaaaatgg tttatttatg ggaatgcaat gataagtatt attttaatgt
     4201 atttttgaat cagataacac taagatatga caatgtttca ctttacccgt atatcgatga
     4261 tctccatcat tttatccttg ctttttgtgt tttgcgacaa tacagataag ccagacggaa
     4321 ccggcgatcc ggccaatctg agcattacgg tactgtcggt cgatatggaa accggcgatg
     4381 tcactttaca agccactgct gataatacgg tcagatacct gctttatatc gatgctgaaa
     4441 caaatccggc agatt
//
LOCUS       DAOQXS010000236         4448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20002_length_4448_cov_4.137947, whole genome shotgun sequence.
ACCESSION   DAOQXS010000236 DAOQXS010000000
VERSION     DAOQXS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20002_length_4448_cov_4.137947"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PKM34_07350"
     CDS             complement(<1..2323)
                     /locus_tag="PKM34_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ83441.1"
                     /translation="MKIFIQISRIITVFLFSLTISASANSIKLNNNENDIRIIQKTGD
                     GFRMEVTLSEFKTMDVKTDKGIFTRIQVPAWSRSRAYGQPEMPVKSELIEIPAGAVVK
                     INLVSWEVNEYRLRDLDISYALMPSQPPVAKSGKIPAFMYERDVYKIHAFIQSEIVSV
                     EELGTMRGVRIGRLDISPFEYNPGEGIVRIYEKLEFDVIFENADPAASERNKLTYGNQ
                     YFAPVFGSLINYTSPKVSERENLTQYPVKYVIVSDPMFEDQLQPFIEWKTKKGFQVIE
                     AYTSDPNVGGTTSQIKAFLQNLYESATPEDPAPSFVLFVGDVAQIPVFYGQAASHETD
                     LYYCEYTGDYFPEMFYGRFSAQNAAQLQPQIDKTLMVEQYTMPVTSYQDTVVMIAGMD
                     GTFGPTHGNGQINYGTINYFNAAHGLFSHTYLYPESGGNADNIRQNISDGVSFANYTA
                     HGSPDGWADPSFTVGDIPSLQNNGKFGLLIGNCCSTSEYEVSNSFAEAIVRAADKGAV
                     GYIGASNSTYWDEDYYFGVGVGTISGDPPSYEETTLGDYDRMFHTHGEPFSEWYTTMD
                     QVIYAGNLAVTLGSPGMAEYYWEAYCLMGDPSLTVYLGEPPVLTASFDPLLPLGNMSF
                     IVNTVPYAYVGLSMNGELLGASLADSNGVATVILTTLPQPGNADVVVTAQNYQPFTGT
                     VLIANPEGPYVSLNQHVPNDENDNGMIEFGEEITLDIELKNWGNDDATGTMASLSTND
                     QYVTISDETENYGTIPAQDSVMQMAAFSFEVAGY"
     gene            complement(2582..3832)
                     /locus_tag="PKM34_07355"
     CDS             complement(2582..3832)
                     /locus_tag="PKM34_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ83442.1"
                     /translation="MLFPLYIAKRYLIAKKSHNIINIISIISVIGVMVGTGALIIVLS
                     VFNGFEGLVMGLFNSVNPDLKVTADEGKVFTLTEEQLSRIKSIPGVAYVVETVEETAL
                     ARFGDSQCIVKLKGVSDEFTEMTPLDSFMLNGKYTLGSPQRPASVIGAGVAYYLGIYP
                     EEFAVPVMLYLPKRTAKTLSGLPDQSFNSRPVRVAGVFSVQQEFDMSYVIAPIELARE
                     LLEYDREVTALELGISDPGATGSIEKQVAEITGPGFLIKDRFQQQATLYKVMKSEKWA
                     VFFILALILVIAAFNMIGSLSMLIVDKKKDIAVLWSLGATKSLIRRIFFTEGMMISLA
                     GGLLGLCLGGLLALLQQEFGLLKLGGGEGNYIVDAYPVKVQLLDFFYVLLTVILIGAA
                     TTWYPVRQISRKYLAQRMGVFFSR"
     gene            4176..>4448
                     /locus_tag="PKM34_07360"
     CDS             4176..>4448
                     /locus_tag="PKM34_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83443.1"
                     /translation="MRDPNEFYKVLVTETIKGTSNSIETWIDHIKRFFNRVTPKVSFG
                     PDPLSTFELSFELEAVEKNYQELLDLPEKIATDKGLKLVICIDEFQN"
BASE COUNT         1135 a         1064 c         1104 g         1145 t
ORIGIN      
        1 cgtatccggc cacttcaaaa ctaaatgcag ccatttgcat gaccgaatcc tgtgccggga
       61 tagttccgta attttccgtt tcatccgaaa tggtgacata ttggtcgttg gttgaaagac
      121 tggccattgt accggtagca tcatcatttc cccagttctt cagctcgatg tcaagggtaa
      181 tctcttctcc gaactctatc attccattat cattctcatc atttggaaca tgctggttaa
      241 gagaaacata cgggccttcc ggattggcaa tcaaaactgt cccagtaaaa ggctggtaat
      301 tctgtgcggt caccaccaca tcggcattgc ccggttgagg caaagttgta aggataaccg
      361 ttgccacgcc gttggaatcg gctaaagagg ctcccagcaa ttcgccattc atcgataagc
      421 cgacataggc atatggcact gtattcacga taaaactcat atttccgagg ggaaggagcg
      481 gatcgaaaga ggccgtcaga accggtggtt cgccgaggta aacggtaagc gacggatcgc
      541 ccatgaggca ataggcttcc cagtaatatt cggccatacc cggcgaaccc agggtaactg
      601 cgaggtttcc ggcatagatc acctggtcca tggtggtgta ccattcgctg aagggctctc
      661 catgggtatg gaacatgcgg tcgtaatcgc ccagggtcgt ctcttcatag gaaggcggat
      721 cgcccgaaat cgttccgaca ccgacaccga aataatagtc ttcatcccag taagtactgt
      781 tggatgcgcc tatgtatccg acagcgcctt tgtcggcagc cctgacgatg gcttcggcaa
      841 aactgttgct cacttcatac tcgctggtgg agcagcaatt accgatcagc aggccaaatt
      901 ttccgttatt ctgcagcgag gggatatcgc cgacggtaaa gctcggatcg gcccagccat
      961 cgggagaacc gtgggctgta tagttggcaa agctcacgcc gtcgctgata ttctgccgga
     1021 tgttgtccgc attgccgccc gattcgggat agaggtaagt atgcgaaaaa aggccgtgcg
     1081 ctgcattgaa ataattgatc gtgccgtaat tgatctggcc gttgccgtgg gttggcccga
     1141 aagtgccatc catcccggcg atcatcacaa cggtatcctg gtaggatgtc acaggcatcg
     1201 tatattgctc aaccataagt gttttatcga tctggggctg aagctgggca gcgttctggg
     1261 ctgaaaaacg gccgtagaac atctcgggga aataatctcc ggtatattcg cagtaataga
     1321 gatcagtctc atggctggct gcctgtccgt agaacaccgg aatctgggct acatcaccaa
     1381 caaacaggac aaaggaaggg gccggatcct cgggagtggc gctttcataa aggttctgca
     1441 gaaaagcttt gatctgggat gtcgtgcctc cgacattcgg atcgctggta taagcttcga
     1501 tcacctggaa tccttttttg gttttccatt ctataaaagg ctgcagctgg tcttcaaaca
     1561 tcgggtcgct gacgatgaca tactttaccg ggtactgcgt caggttctcc ctttcggaaa
     1621 ctttcggtga agtataattg atgagtgatc cgaaaacggg ggcaaaatat tggtttccgt
     1681 aagtcagctt gtttctttct gaagcagcag gatcagcatt ttcaaaaatg acatcaaact
     1741 ccagtttttc gtaaatcctg acgatccctt ctccgggatt gtattcgaag gggctgatat
     1801 ccaggcgacc gatcctgacc cctctcatgg ttccaagttc ttccaccgaa acgatttccg
     1861 attgaatgaa agcatggatt ttgtaaacat ccctttcata cataaacgcc ggaatcttac
     1921 ccgatttggc gaccgggggc tggctgggca tcagggcata ggaaatgtcc aggtctctca
     1981 accggtattc gttcacttcc cagctgacca gattgatttt cacaacagcg cctgccggta
     2041 tttcgatcag ctcgcttttt acgggcattt ccggctgtcc gtatgcacgc gacctcgacc
     2101 atgccgggac ctggatacgg gtgaagatcc ctttatcggt cttgacatcc atcgttttaa
     2161 actctgataa agtcacctcc attctgaaac catcgccggt tttttggata atccggatat
     2221 cgttttcatt gttgttcagc ttgatggaat tggcactggc tgaaatagtt aacgaaaaaa
     2281 ggaatacagt aataatcctt gagatttgga taaaaatttt cataaacatt tgattaatta
     2341 ctcattatgg ttaagcgtcg aaagggaaaa agtcattatt aataaaacaa aagtagattt
     2401 tttcaggtaa tgatcttatg atttcattga aatatgatga atatcgtatt aaggatgtct
     2461 caaaagtcta tatattcact tttttaggta gtccgacgac taaagccgtc ggttccaacc
     2521 cttataaaac attcggaacc gatgggttta cccgtcggaa gaaaatgccg gctatcattc
     2581 cctagcgact aaagaacacc cccatccgct gggccagata cttgcgtgag atctgccgca
     2641 ccgggtacca ggtggtggcg gcgccgatca ggatgacggt aaggagaacg tagaaaaaat
     2701 cgagcagttg gactttcaca gggtaggcgt cgacgatata attgccttca ccgccgccca
     2761 gtttcagcag gccgaattcc tgctggagca aggccagcaa tccgcccagg cagaggccga
     2821 ggagtcctcc tgccagggat atcatcatcc cttcggtgaa gaagattcta cggatcagcg
     2881 atttggtggc cccgaggctc cagagcaccg cgatatcttt tttcttatcg acgatcagca
     2941 tcgacagcga gccgatcatg ttaaaagccg cgatgacaag gatcagggcc aggatgaaga
     3001 agacagccca cttttccgac ttcatgactt tatagagagt cgcctgttgc tggaaccggt
     3061 ctttgatcaa aaagccgggt ccggtaattt ccgccacttg cttttcgatg ctgccggttg
     3121 ctcccgggtc ggagatgccc agctcgaggg cggtcacttc ccggtcgtat tcgagcagtt
     3181 cgcgggccag ttcgataggg gcaatcacgt acgacatgtc gaattcctgc tgaacggaga
     3241 agacgccggc tacgcgcacc ggccggctgt tgaacgactg gtcgggcaag cctgagagcg
     3301 ttttggcggt ccgttttggc aggtagagca taacagggac ggcaaattcc tccgggtaaa
     3361 tgcccaggta gtaggccacg ccggctccaa tcaccgatgc ggggcgctgc gggctgccca
     3421 gggtgtactt tccgttgagc atgaagctgt cgagcggcgt catctccgta aattcatcgc
     3481 tgaccccttt gagcttcaca atgcactggc tgtcgccgaa acgggcgagg gctgtttctt
     3541 cgacggtttc aaccacatag gcaacgcccg gaatgctttt gatccggctc aactgttctt
     3601 cggtcagcgt gaacacttta ccttcatccg ctgtcacttt caggtcggga ttaacgctgt
     3661 tgaacagccc catgaccagt ccttcgaacc cgttgaatac cgacaaaacg atgatcagtg
     3721 cgcccgttcc caccatcacc ccgatgaccg aaatgatcga gatgatgttg atgatgttgt
     3781 gcgatttttt cgcgataagg tatcgttttg ctatgtagag gggaaagagc atggtgcaaa
     3841 tttaggcatt ttataagacg aaacagcgcc gcagctttca gttacaaacc cattagtttg
     3901 caaccgatag gttacatata aatgttgtat ctttgcttct ccaccatttc aatcaaattt
     3961 atatggaaag tccgttcgta tttggaagaa aagccactgg taataacttc actgacaggg
     4021 aaaaagaaac cgggagatta atctcaaatt ttaataaccg tgtaaacacg atattgatct
     4081 cacccagacg atggggaaaa tcttcgttgg tgaggagggc cggcgaactg gccgggaatg
     4141 aatcattgaa agtcatttat tttgattctt tcagtttgcg cgatcccaat gaattttata
     4201 aggttctggt cacagaaact atcaaaggaa catctaattc tattgaaaca tggatagatc
     4261 atattaagag attttttaac cgggtaaccc caaaggtatc ttttggccct gatccgttaa
     4321 gcacttttga actttcattt gaactggaag cagttgaaaa aaattatcag gaattgctcg
     4381 atctgcctga aaaaattgca acagacaaag gcctgaagct ggtaatttgc atcgatgaat
     4441 ttcaaaat
//
LOCUS       DAOQXS010000237         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20020_length_4445_cov_3.785194, whole genome shotgun sequence.
ACCESSION   DAOQXS010000237 DAOQXS010000000
VERSION     DAOQXS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20020_length_4445_cov_3.785194"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKM34_07365"
     CDS             complement(<1..416)
                     /locus_tag="PKM34_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ83444.1"
                     /translation="MKTLKMNTERIIPILLVLLPVMVLLIIAGCSCPVKKEETVTKEP
                     PQSFVVHPEWSRNATIYEVNIRQFTPEGTFQAFTAHIPRLKKMGVDILWLMPIHPIGE
                     RGRKGALGSYYSVKDYKGINPEFGTLEDFKQLVGKA"
     gene            complement(480..>2696)
                     /locus_tag="PKM34_07370"
     CDS             complement(480..>2696)
                     /locus_tag="PKM34_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ83445.1"
                     /translation="IIDSVSLYGRILKLSGDKQVAAINVGTDAPPLMELKIWSGGQAL
                     SVPVMKSRKADILYSFDPGNSVYEKVELAGEFNGWTPSRTPLKFENGNWVTTLSLNPG
                     RYQYQLVTDGKWRIDPANPDSVDNNNGGFNSVLKAGPSDRSAQPQLFASGYDLKSNEI
                     GITWTSKPDEFLVFWQNVRLDETYLEQEEDELNIFIPEEAKRFGRSFIRVFAYNGYGL
                     SNDLLIPLQNGKVVLDPADLTRADRQSMIIYNVFTDRFFNGNPSNDKRVGNPQVVLPK
                     ADYFGGDIAGVTQKIREGYFNDLGVNTLWISPVVLNPDGAYGQWNDPKTKFSAYHGYW
                     PVSFTLIDPRMGTPEEFREMVSEAHARGINVLLDFVAHHVHELHPYYIEHPSWTTSLY
                     LPDGSLNTEKWDEHRLTTWFDVFLPTLDLQQPEVTEMLSDSAVWWIKHYNLDGFRHDA
                     TKHVPEIFWRTLTRKLKQQVLMEEGREVYQIGETYGGPELIASYIGSGMLDGQFDFNV
                     YDAAIGVFARESDPFTNLDKTLQTSLDFYGYNNQMGYITGNQDRGRFISYAGGDLKFG
                     ENAKAAGWTREIGVGDTIGYRRLSMLTAFNMTIPGLPVIYYGDETGIPGGNDPDSRRQ
                     MKFSDLTPLEMRTRNLATDLTRIRKDNLALVYGSFSTVEVSDKIYVFVRKYFDNEVIV
                     IFNKNNRLERIEADLPHWMDCKNMKAMFGHDFKINERKLSVEIQPWSFEIIVNTRP"
     assembly_gap    2697..2706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2983..>4445
                     /locus_tag="PKM34_07375"
     CDS             2983..>4445
                     /locus_tag="PKM34_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ83446.1"
                     /translation="MKKKKNNIRAIPENPVIYEINTWVWLRELGEKYRKKINLGNVPA
                     KEYDNLAATGIDTVWLMGVWKRSPAGLEIAKNNEGIMNDLRNALPDLTDDDISGSPYC
                     IMDYQADKKIGGEKGLAKARKELADRGIRLILDFVPNHVAPDHPWTVKNPGFFINGSE
                     QDLAEHPEAFLKAGNHIMARARDPYYPPWPDVLQLNVFNRGLRDELVKTLDHVASQCD
                     GIRCDMAMLVMNDIFSKTWGDKAGPAPGTDFWKHVIPAIRKKHPEFVFIAEVYWDMEA
                     ALFNQGFDYCYDKRYYDDLKEGPEKARQHLEGMTLYEGKLLRFLENHDEPRAASLFHP
                     AKNMALALASLTLPGARLFHDGQLEGRSVKVPVFLSRRQKEPINEPLNRFYLLLLKIL
                     KYHEVRYGTWAPCELSGWHDNQTCQNMLAWEWTGEHENLIIVVNLSDDPSQAHLKSGY
                     PWLPGKTYQLFDVTSGELYRRDGEEMNDPGLYIILQG"
BASE COUNT         1139 a         1117 c         1095 g         1084 t
ORIGIN      
        1 tgcgcttttc cgaccagctg tttgaaatct tccagtgtgc cgaattcggg attgatccct
       61 ttgtaatcct ttacggaata gtaacttccc agcgcccctt tccggcctcg ctcccctatc
      121 gggtgaatgg gcatcagcca cagaatatcg acacccatct ttttcaggcg ggggatatgg
      181 gctgtgaaag cctggaaagt tccctcgggc gtgaattgcc tgatattaac ttcataaatc
      241 gttgcattac ggctccattc cgggtgtacg acaaaacttt gcgggggctc tttggtcacc
      301 gtttcctctt tctttacggg acaggaacag ccggcaataa ttaataaaac catcaccggt
      361 aaaagtacca ggagtatagg aatgatcctt tcagtattca ttttcagggt tttcatttta
      421 atttttttat aatgattaat tagaacacgg atgaaacgga ttaaacggat ttatgcggat
      481 cagggcctgg tgttgacgat gatctcaaat gaccagggtt gaatctctac agatagtttc
      541 cgttcattta ttttgaaatc atgaccgaac atcgctttca tatttttaca atccatccag
      601 tgaggaagat cggcctcaat tctttccaac cggttgtttt tattgaagat gacgatcact
      661 tcattatcaa aatatttacg aacaaaaaca taaatcttat ccgaaacctc cactgtcgaa
      721 aaactgccgt agaccagcgc caggttatct ttccggatac gggtcaggtc ggtggccagg
      781 tttcttgtcc tcatctccag cggggtcagg tcgctgaatt tcatctgccg gcgcgagtcg
      841 ggatcattac cgccgggtat gccggtttca tcgccgtaat agatgaccgg aagccccgga
      901 atggtcatat tgaaggctgt cagcatcgac aagcggcggt agccgatcgt gtcgcccacc
      961 ccgatttcgc gggtccagcc ggccgctttg gcattctcgc cgaatttcag gtcgccgccg
     1021 gcatatgaaa taaagcggcc ccggtcctgg ttcccggtga tatagcccat ctggttgtta
     1081 taaccgtaaa aatccaggct ggtctgaagg gtcttgtcaa gattggtgaa aggatcggat
     1141 tcacgggcaa aaacgccgat ggccgcatcg taaacattaa aatcgaactg gccgtcgagc
     1201 atgcccgaac cgatatagct ggcgatcagc tccgggccgc cgtaggtctc gccgatctgg
     1261 tagacctccc ggccttcttc catcaatacc tgctgcttca gcttacgggt aagcgtccgc
     1321 cagaaaatct ccgggacgtg cttcgtggca tcgtggcgga atccgtccag gttgtagtgc
     1381 ttaatccacc agacagccga atcggacagc atctccgtaa cttcaggctg ctgcaggtcg
     1441 agcgtcggga ggaaaacgtc gaaccaggtc gtcagccggt gctcgtccca cttttcggtg
     1501 ttgagcgatc cgtcgggaag gtaaaggctg gttgtccacg atggatgttc gatataataa
     1561 ggatgcagtt catggacatg atgcgcgaca aagtcgagca atacgttgat tcccctggca
     1621 tgcgcttcgc tgaccatctc cctgaattcc tccggcgttc ccatccgggg gtcgatcagg
     1681 gtgaacgaaa cgggccagta gccatggtaa gcggagaact ttgtcttcgg atcgttccac
     1741 tggccgtaag cgccatccgg gttaaggaca accggggaga tccacagcgt gttcaccccc
     1801 aggtcattaa aatatccttc cctgatcttc tgagtcacac cggcgatatc accgccgaaa
     1861 tagtcggctt tgggcaaaac cacctgaggg tttccaaccc ttttgtcatt cgacgggttt
     1921 ccgttaaaga accggtctgt aaaaacattg taaatgatca tcgactggcg gtcggcccgc
     1981 gtgagatcag caggatccag cacgactttt ccgttttgaa gcggaataag caagtcgttt
     2041 gataaaccgt atccgttgta agcaaaaaca cggatgaacg aacggccaaa cctcttcgct
     2101 tcttccggaa taaaaatatt aagttcgtct tcttcctgtt caagatacgt ttcatcaaga
     2161 cgtacatttt gccagaaaac aaggaattca tccggttttg atgtccatgt gatgccaatt
     2221 tcattgcttt tcaagtcata cccggaagca aagagctgcg gctgtgccga acggtcggac
     2281 ggtcccgctt tcaaaaccga attaaaaccg ccattgttgt tatcaaccga atcgggatta
     2341 gccgggtcga tcctccattt accgtcggtc acaagctgat actggtatcg tccgggattc
     2401 agcgaaagtg tcgtcaccca atttccattt tcaaatttca aaggcgtcct cgatggtgtc
     2461 cagccattaa attctcctgc cagttccact ttttcataaa cactatttcc cgggtcaaat
     2521 gaataaagaa tatccgcttt cctgcttttc atcacaggca ccgacagtgc ctgcccgccg
     2581 ctccagatct tcagttccat cagcggtggc gcatctgtcc cgacattgat cgctgcaact
     2641 tgtttgtcgc cggaaagttt cagtatcctg ccatagaggc ttaccgaatc gatgatnnnn
     2701 nnnnnncgcc aggggccagg tcggccagga tgatctccgg ctgccctttg tcgagccgca
     2761 caggcatggc aatacccgga atgaagccgg tttccggaat aggtgtacgc ttcgggctgc
     2821 aggcaacaac cgaagccgtc agcagaataa acagtaatct tttcatggga aaagcatttg
     2881 ggattgcaaa tatcgtaaag aaaaactaaa aaaaattata cttttatagg gtcttacttg
     2941 cgttactaat gaagaaatga agtttccatt aatcccaaca caatgaaaaa gaaaaagaat
     3001 aatataaggg ccatccccga aaaccctgtt atttatgaga ttaacacatg ggtctggctc
     3061 cgggaactgg gggaaaaata caggaaaaag attaacctgg gcaatgtacc ggcaaaagaa
     3121 tacgacaacc tggcggcgac gggaattgat acggtctggc tgatgggtgt ctggaaaaga
     3181 agcccggcag ggctggaaat tgcaaaaaac aatgaaggga tcatgaatga tctccggaat
     3241 gctcttcccg acctcacgga tgatgacatt tccggctcac cttattgcat catggattac
     3301 caggcggata agaagatcgg cggggaaaaa gggcttgcca aagcgaggaa agagctggcc
     3361 gaccgaggca taaggcttat cctcgacttt gttccgaacc atgtggcccc cgaccatccc
     3421 tggacggtca aaaatccggg ttttttcatc aatggcagcg aacaggacct ggcagaacat
     3481 ccggaagcat ttctgaaggc aggaaatcac atcatggcaa gggcccgcga cccctattat
     3541 cctccttggc ctgatgttct gcaattgaat gtatttaacc ggggcctgcg tgacgaactc
     3601 gttaaaacac tcgatcacgt tgcttctcag tgcgacggca tccggtgcga catggccatg
     3661 ctggtcatga atgacatatt ttcgaaaaca tggggagata aagcaggccc ggccccggga
     3721 accgactttt ggaaacatgt tataccagct atccggaaaa aacatcccga atttgttttt
     3781 attgcagagg tatactggga tatggaagcg gcgcttttta accagggatt tgattattgt
     3841 tacgacaaga gatattacga tgacctgaaa gagggccctg aaaaagcccg gcaacacctg
     3901 gaagggatga ccctttacga aggtaaactg ctccggttcc tggaaaatca cgacgaaccc
     3961 agggctgcct ctctgttcca cccggcaaaa aatatggccc tggcactggc ttccctgacc
     4021 cttcccgggg ccaggctttt ccacgacggc cagctggagg gaagaagcgt taaggttccc
     4081 gttttcctgt ccaggaggca gaaggagcca ataaatgaac cccttaacag attttacctc
     4141 ttgctcctga aaatcctgaa gtaccacgag gtcagatacg gaacctgggc gccctgtgaa
     4201 ctttccggat ggcacgataa ccagacgtgc cagaatatgc tggcatggga atggaccggg
     4261 gaacatgaaa accttatcat tgttgttaac ctcagcgatg atccatccca ggcacacctg
     4321 aaatcgggct atccctggct cccgggtaaa acttaccaac tgttcgatgt tacttccggt
     4381 gaattatacc gccgcgacgg ggaagagatg aacgatcccg gcctctatat cattttgcag
     4441 ggatg
//
LOCUS       DAOQXS010000238         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20035_length_4443_cov_3.310392, whole genome shotgun sequence.
ACCESSION   DAOQXS010000238 DAOQXS010000000
VERSION     DAOQXS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20035_length_4443_cov_3.310392"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PKM34_07380"
     CDS             <1..748
                     /locus_tag="PKM34_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNQ83447.1"
                     /translation="EKRIDEPSSPKLLRFRPRWIIAAAASLILLAGVASVLFYTSRTI
                     VSAPGQHLSHILPDGSRVELNADSKISYKPYRWMFSRIIELSGEGFFEVEPGRTFSVV
                     SAQGTTTVLGTTFNIYARADEYKVNCLTGKVKVVSTTESFVVLSPSQSAVIENDGKIL
                     VSRQSNEANPVGWLDNMFDFKAVPFTRVMEEIGRQYGVSIQADIPEGIIYTGHFSKNR
                     PLEETLGVVCKPFGFTFARIAENEYIISQN"
     gene            752..3259
                     /locus_tag="PKM34_07385"
     CDS             752..3259
                     /locus_tag="PKM34_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83448.1"
                     /translation="MTSPAKAFAFLICLVIPVLASGQKLSLEVQGKPASEVLYDFSGR
                     YGLQISFNEKSLSQYKVNITGEFSSPEEAIRKVIENLPFELEKQGDVFIIIPQKKSPK
                     KRTYSITGQVIEKGTREPLPYSHVIINNTGIATDIKGSFSYISSTDSVFQIKATHLGY
                     FILDTLVMGNRNVQFSLSPNFIGLNEVIITHSVVERASLIGREPGVMKLNHQVANFLP
                     GFGDNSVFNLLRLQPGILASGEQTNELIVWGCYEGQSKVIFDGFTLYGLKNFNDNIST
                     FNPLMAKDIEVYKGGFDARYGERVGGIVNITGKNGNTEKTSFTVDINNMTMNGMVEIP
                     LFKTGSLIFALRHTYYELYNPGDVNDLFSRGRGNNAVENVDVNIIPDYRFGDMNLKYS
                     ARIRNTDLFYISLYSGNDRFSYSINEPFNHIIIKKNTSEKNRQSGGSVFYGKSWKNGY
                     STHFSLNYTVLNSTFTDNLEIEKVIQQKTEQRIDLYAFNSIRENSLRVDNRFPVHESH
                     ILEGGMIYMANRIELAEDTFDIPRVRFDEEAKRMTLYFQDNIKLLNIIDIKAGFRLTR
                     ANNLKKFYFEPRLSASVRLGESFKINGAWGLYDQWVTKSSVLDDMGNYRYIWTVCDNE
                     SIPVLKAYHYVLGTSYNHNDLTVSIEGYYRNVSGLTRYIRNTALNIEGIYEGIGRSYG
                     VDLMIKKDYKGHSAWISYTVGKTEELFEYFKKDEYRRAPQDQRHEVKAAILVNLDPFY
                     ISADYVFGSGFPSAPAILAEQADDLTYSRLDASFIYKFLDRRLKGEVGLSVLNVMNTE
                     NIKYANFERVSTNADNSVNLYSEAIPITPALYLKLAM"
     gene            3306..4061
                     /locus_tag="PKM34_07390"
     CDS             3306..4061
                     /locus_tag="PKM34_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ83449.1"
                     /translation="MTNIYNSGKLKASGKATGTFYFKHFGVKDDRSTMKVGTDAVLLG
                     AAVDVAGAASILDIGTGCGVIALILAQRSDALIEAIDIDEESVKQAKENVVKSPWKER
                     INVINVSLQDYTDVCSKKYDLIVSNPPYFSNSLKSPSQRRNLSRHDHSLGYDDLIRIA
                     SQILNPNGSFWVILPEAKCEEFIQKAGQNNLFPSRLLYIIPKPNRNIQLVIIRFDKCS
                     VVAPVIQTLHIKNENNSYSKEYKNLTGEFYLDF"
     gene            complement(4232..4381)
                     /locus_tag="PKM34_07395"
     CDS             complement(4232..4381)
                     /locus_tag="PKM34_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83450.1"
                     /translation="MFFQVAYDFNGKDNPILQSDASTGQSVGAGGVFFRRVSPSALEG
                     FSSDA"
BASE COUNT         1231 a          979 c         1068 g         1165 t
ORIGIN      
        1 ggagaagagg attgacgagc cgtcatcccc gaagctcctc cggttcaggc cccgttggat
       61 catcgccgcc gccgcatctc ttatcctgct ggcaggagtt gcttccgtgc ttttctatac
      121 ctcgcgaact attgtcagcg ctccgggaca gcatctttcg catattctcc ccgacggatc
      181 acgcgttgaa ttaaatgctg acagtaagat cagttacaag ccttaccggt ggatgttttc
      241 caggattata gagctttccg gcgaaggatt cttcgaagtt gaacccgggc gtaccttctc
      301 tgttgtctcg gcccaaggga ctacgactgt tctgggcact acctttaata tatatgcgag
      361 ggccgatgaa tacaaggtca attgcctgac cggaaaagtg aaagtcgttt cgaccactga
      421 atcctttgtc gttctcagtc ccagccaatc ggcggttatt gaaaatgatg gaaagatcct
      481 ggtttcgcgc caatccaatg aggccaatcc ggtcggttgg ctggataata tgttcgattt
      541 taaagccgta ccgtttaccc gggtaatgga agaaattggg agacaatacg gagtatcgat
      601 acaagccgat ataccggagg ggatcattta cacaggacat ttttccaaaa acaggccgct
      661 ggaggagacg ctcggggtgg tttgtaaacc atttggattt acttttgcga ggattgctga
      721 aaacgagtat ataatttctc agaattgacg gatgacaagt cccgccaaag catttgcctt
      781 cctcatctgc ctggtcatac cggtattggc ctcaggccag aagctcagcc tggaggtaca
      841 ggggaagccc gccagcgaag tattatatga tttttcaggc cggtatggtc ttcagatatc
      901 gttcaatgaa aagagtttat ctcaatacaa ggttaatatc accggtgaat tctcttctcc
      961 ggaagaagcg atacggaagg taattgagaa cctgcctttt gaacttgaaa aacagggtga
     1021 tgtattcatc atcatccccc aaaaaaaatc tccgaaaaaa aggacctaca gtatcacagg
     1081 gcaggtcatc gagaaaggga cacgggagcc cctgccctac tcgcacgtca tcatcaacaa
     1141 caccggcata gcgactgata tcaaaggcag cttcagttat atttcctcaa ccgacagtgt
     1201 ttttcagatc aaagcgaccc atctcggtta ctttattctc gacacgctgg ttatggggaa
     1261 ccggaatgtg cagttcagtt tatcacccaa ctttatcggt ttaaatgagg tcattatcac
     1321 ccattcggtc gtagaaaggg catccctgat cggccgtgag cccggagtaa tgaagctgaa
     1381 ccaccaggtg gccaatttcc ttccagggtt cggcgacaac tccgttttta acctgctccg
     1441 gcttcagccc ggcatcctcg cctcaggaga acaaaccaac gaactgattg tctggggatg
     1501 ttatgaaggg cagagcaaag tgatcttcga cgggttcaca ctttacgggt taaagaactt
     1561 caacgacaat atcagcacct tcaatcccct gatggccaaa gatattgagg tttacaaagg
     1621 aggttttgat gcccgatacg gggaacgcgt cgggggcatt gtcaatatca ccggtaaaaa
     1681 cggaaatacc gaaaagacct cattcaccgt cgacatcaac aacatgacta tgaacggcat
     1741 ggttgaaata cccttgttta aaacagggtc actgatattc gcccttcgtc atacgtatta
     1801 tgagttgtat aacccggggg acgtcaatga ccttttttca agagggaggg gaaataatgc
     1861 ggtggaaaat gtggatgtca acattattcc agattaccgt ttcggggata tgaacctgaa
     1921 atattcggcc cgcatcagaa acacggatct tttttatatc agtctttata gcgggaatga
     1981 ccgtttttcg tattccatca atgaaccttt caaccatatc atcatcaaaa aaaacacttc
     2041 tgaaaaaaac aggcaatcgg ggggatcggt attttacgga aaatcctgga aaaatggata
     2101 ttcgacccat ttcagcctga attataccgt tttaaattcg acatttacgg ataacctgga
     2161 gatcgaaaag gtgatccagc aaaaaacgga gcagaggatt gacttgtatg cgtttaactc
     2221 gatccgggag aattcgttgc gggttgataa ccggtttccg gttcatgagt cacatatcct
     2281 ggaaggagga atgatttata tggccaaccg gatcgaactg gcggaagaca cttttgacat
     2341 accgcgagtt cggttcgacg aagaggcaaa aaggatgacc ctatactttc aggacaatat
     2401 caagctattg aatattattg atataaaagc cggtttccgt ttaacccgtg cgaataatct
     2461 gaagaaattt tactttgaac ccaggttatc ggcatcggtc aggctgggtg aatcctttaa
     2521 aattaacggt gcctggggac tttatgacca gtgggtaaca aagagttccg ttttggatga
     2581 tatgggcaat taccggtaca tctggacggt ttgcgacaac gagagcatac cggtccttaa
     2641 ggcttatcat tatgttctcg ggacttccta caaccataat gatttgactg tcagcattga
     2701 aggatattac cgcaatgtat caggactgac gcgctatatc cggaatacgg cgctgaacat
     2761 cgaagggatt tacgaaggaa ttggccgttc gtacggagtg gacctgatga tcaagaagga
     2821 ttacaaagga cattcagcat ggatttcgta cactgttggc aaaacggagg agttattcga
     2881 atatttcaaa aaggatgaat accgccgtgc gccgcaggac cagcggcatg aagtgaaagc
     2941 cgcgatcctg gtaaacctgg atccttttta tatttctgcc gattatgttt tcggatcggg
     3001 ttttccctca gctcccgcta tcctcgccga acaggctgat gacctgactt acagccggct
     3061 ggacgcatcg tttatctata aattccttga caggcggtta aagggcgagg tcggactatc
     3121 ggtattaaat gtgatgaata cggagaatat caagtacgcc aatttcgagc gggtctcgac
     3181 gaatgctgac aattcggtca acctgtattc agaggctatt ccgattacgc cggcattgta
     3241 tttgaagctg gcaatgtaga ggacagcctt atccgggcga agcgagggat tggacagctg
     3301 gtaatatgac aaatatttat aatagcggaa agttgaaggc gagcgggaaa gcgaccggca
     3361 ccttttactt caagcatttc ggggtgaagg acgaccggag caccatgaag gtgggtacgg
     3421 atgctgtatt gctgggtgcc gccgttgatg tggccggagc cgcttcgata ctggatatcg
     3481 gaacgggttg cggcgttatt gcgctgatac tggcgcagcg atccgatgct ttgatcgaag
     3541 ctatcgatat cgatgaagaa tcggttaagc aagcaaagga gaatgtagtg aagagcccgt
     3601 ggaaagagag gataaatgtt attaatgttt cgctccagga ttataccgat gtttgtagca
     3661 agaaatatga ccttatcgtt tccaatcccc cctatttttc gaattcatta aagtctcctt
     3721 cacagagaag gaatctttcg cggcatgacc attcgctcgg ttatgatgat cttattcgta
     3781 ttgccagcca aattttaaat ccgaatggca gcttttgggt tattttaccg gaagcaaaat
     3841 gtgaggagtt catccagaag gccggacaaa acaatttatt tccaagcagg ttgctttata
     3901 tcatcccaaa acccaacagg aatattcaac tggttatcat ccggtttgat aaatgcagcg
     3961 tggtggctcc ggttattcaa acccttcata ttaaaaacga aaataactct tattcaaagg
     4021 aatacaaaaa tctcaccggg gaattttatc tcgattttta aggttttgtt tatcccctac
     4081 agggaattat tgcttattgt aattgatcct gcaacaaaat tttatcgcct acggcgaaaa
     4141 tccctacggg gaattattga tttttgttat tgatccggca acagaatttt atcgcctgcg
     4201 gcgaaaacct ctagcctaat aaccgacgaa tttacgcgtc ggaagaaaat ccctccagcg
     4261 ccgacggact tacccgccgg aagaaaaccc ctccagcgcc gacggattga cccgttgaag
     4321 catcagattg caaaatcgga ttatctttac cgttgaaatc atacgcgact tgaaaaaaca
     4381 tctactggaa atcatcgttt ttttgtgcgg tgcggttgtc atgatctttg agcttgccgg
     4441 atc
//
LOCUS       DAOQXS010000239         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20101_length_4432_cov_4.025131, whole genome shotgun sequence.
ACCESSION   DAOQXS010000239 DAOQXS010000000
VERSION     DAOQXS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20101_length_4432_cov_4.025131"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKM34_07400"
     CDS             complement(<1..398)
                     /locus_tag="PKM34_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNQ83451.1"
                     /translation="MKKALIVGSGLGGLTAAIRLAKIGYQVEMVEKYHQPGGRLNQLK
                     KDGFTWDMAPTFFSMSYEFKEFVNSTGIKMPFEFVELDPLYAVNFANSDKFYLIHKNL
                     EKLAKEFEAIEPGFEAKMKRYLEGAGKLFH"
     gene            471..1943
                     /gene="crtI"
                     /locus_tag="PKM34_07405"
     CDS             471..1943
                     /gene="crtI"
                     /locus_tag="PKM34_07405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNQ83452.1"
                     /translation="MPGKVAVIGAGIGGIAVAVRMALKGYRVDVFEKNSYPGGKMAEL
                     RHGGYRFDTGPSLFTLPGLVSELFELAGESMDDHLPYRKLDETCRYFFRDGMVIHAFS
                     TPERFADELQRKAGEDPARVLKYLSDSRKIYELTDPVFISNSLHLKGNYLKTPFLKAF
                     LQLYRLKPFSTLHRVNRNYFSHSNTVNIFDRFATYNGSNPYLTPATLMVIPHLEHNTG
                     AYFPEKGIYGIASALTELAGKKGVKFHYNSKVDEILVKNGRISGIRVSGQGEIEYDMV
                     ISDADIWFIYRHLLKQKPFPEKWFCHERSSSALIFYWGMKTQSENLGLHNILFAKDYK
                     EEFDCLFNKKVIHDDPTVYLFISSKMVKDDAPGGCENWFVMINTPENCGQDWDSMITA
                     ARAKIGEKILRMTGIDAGRYRQFEFVMDPRGIEEKTASYRGSLYGNSSNSMFAAFRRH
                     PNFSGIKGLYMVGGSVHPGGGIPLCLSSAKIVSGMIPDLK"
     gene            1951..2601
                     /locus_tag="PKM34_07410"
     CDS             1951..2601
                     /locus_tag="PKM34_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNQ83453.1"
                     /translation="MIENVRKYPVIMIAVISFYYMVGIIGLSLETTRELFRALVPFTL
                     LFSLYFLWLFHENPGLRFYLVSLVIFLLGFFIEVVGVKTGWFFGHYVYGETLGFKILS
                     TPLMIGVNWLFLIISCWSLTGLFLRNRWLRYKTGALFMVIYDIFLEPVAVRLDMWSWH
                     GDKIPLQNYIGWYITSWLLFIILDQSRVSVKNKIAPSLFIIQFLFFVILNIINFLS"
     gene            2602..3273
                     /locus_tag="PKM34_07415"
     CDS             2602..3273
                     /locus_tag="PKM34_07415"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNQ83454.1"
                     /translation="MGVIIALTILVAWAAHLAYILAGLEIDYASPWFYFHILLQAYLY
                     TGLFITGHDAMHGVVSSNRPVNQAIGYTVVFLYAGMWYRKLIRYHGMHHQFPGTERDP
                     DYSTGSQNFFVWWFRFMIHYITWIQLLIMAALYNILTIWLSDRQVILFWVLPAVLSTF
                     QLFYFGTYVPHKRPHTGQMGPHKARTLAKSHLLAMITCYFFGYHREHHESPRTPWWQL
                     YRLKS"
     gene            3278..3895
                     /locus_tag="PKM34_07420"
     CDS             3278..3895
                     /locus_tag="PKM34_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ83455.1"
                     /translation="MIRAGHRKFFVIFFDFYTKWMLRRHFQRIIIYTSPDETDKPVMM
                     IGNHFSWWDGFIANYINLELFRRKIHIMMLEEQLRGRMFLNKAGAFSIRKNSRSAIES
                     LDYTVGLLENRDHLVVLYPQGEFQSVYSSRIRFQPGIMRVLKGLRKPVQMVFYAALTD
                     YFSSFRPHLSIYLETVDPDDLQDIKALENAYNDFYNACKEKQMKE"
     gene            3892..>4432
                     /locus_tag="PKM34_07425"
     CDS             3892..>4432
                     /locus_tag="PKM34_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83456.1"
                     /translation="MMEYLAYAAVAYLALRLLVILVNQFSGTEMTISPGSSSKTVSAL
                     IPARNEEENIGRLLDDLSASEYPHLRIFVYDDESEDRTADIVIEKSRMDDRIILIRGR
                     IPGPGWLGKNHGCSRLAEAAVGDYFLFIDADVRVSKTLINSAVQSMEQHQLALLSIFP
                     VQMMNSTGEWLTVPLVNRIL"
BASE COUNT         1092 a         1017 c         1072 g         1251 t
ORIGIN      
        1 tcgtgaaaaa gctttccggc gccttccagg tatcgtttca ttttggcctc aaatcccggc
       61 tcgatggctt caaattcttt cgccagtttt tcgagattct tatgaatgag atagaacttg
      121 tcgctgttgg caaagttgac ggcatacagc ggatcaagct caacgaattc aaagggcatt
      181 ttaatgcccg ttgaatttac aaactctttg aattcatagc tcatgctgaa gaaagtggga
      241 gccatatccc atgtgaatcc gtcttttttc aactgattaa gtcttcctcc gggctggtgg
      301 tatttttcaa ccatttcaac ctgataaccg atcttggcta aacggatggc ggcggtaaga
      361 ccacctaaac ctgaaccgac aatgagcgct tttttcattt tttgatttgg ttcccgaaat
      421 taagaaaaac aagagacttt aaaacatatc tttgtttaaa atgttcaaaa atgcccggaa
      481 aagttgcggt aatcggtgct gggataggcg gaatagccgt tgctgtcaga atggcattga
      541 aaggataccg ggttgatgtt tttgaaaaga acagttatcc gggcggtaaa atggccgaat
      601 taaggcacgg aggataccgt ttcgatacag gcccatccct ttttaccctc cccgggcttg
      661 tttccgaact gtttgaactg gccggcgaat cgatggacga ccatcttcct tatcgtaagc
      721 tggatgaaac ctgccgatat tttttccgtg acggaatggt gatccatgct ttttccactc
      781 cggaaagatt tgcggatgaa ctgcaacgga aagccggtga agatccggcc cgtgtgctga
      841 agtacctgtc tgacagcagg aaaatttatg aactgactga tcccgttttc atcagcaatt
      901 cccttcacct gaaagggaat tacctgaaaa cccctttttt gaaagcattc ctgcagctat
      961 accggctgaa gccgttcagc acccttcacc gggtaaacag gaattacttc agccacagca
     1021 atacggttaa tatattcgat cgctttgcca cctataatgg atcgaatcct tacctgaccc
     1081 ccgcaaccct gatggttatc ccgcatttgg agcacaatac cggcgcttat tttcctgaaa
     1141 aagggatcta tggcattgct tcagcactga ctgaacttgc cgggaaaaag ggcgttaagt
     1201 tccattataa tagtaaagtg gatgaaatcc tggtaaaaaa cggaaggatt tcaggtatca
     1261 gagtgtccgg ccagggtgaa atagaatacg acatggttat cagcgatgcc gacatctggt
     1321 tcatttacag gcatttgttg aagcaaaagc cttttccgga aaagtggttc tgtcatgagc
     1381 gttcctcctc cgctttgata ttttactggg gaatgaaaac acaatcggag aatctcgggc
     1441 ttcataatat ccttttcgca aaggattaca aagaggaatt cgattgcctg ttcaataaaa
     1501 aggtgatcca tgatgatcct acggtttacc tgtttattag cagcaagatg gtaaaagatg
     1561 atgcgcccgg gggatgtgaa aactggtttg ttatgatcaa tactccggag aattgcgggc
     1621 aggactggga ttcgatgatt accgcagccc gggcgaagat cggggagaag atactgagaa
     1681 tgaccggaat tgatgccgga cgttaccggc agtttgagtt tgtcatggat ccgcggggta
     1741 ttgaagagaa aaccgcttcg taccggggat cactctatgg aaacagttct aattcgatgt
     1801 ttgccgcttt ccggcgccat ccgaattttt ccggtattaa aggcctgtac atggtcggtg
     1861 gcagtgtcca cccgggagga ggaatccctc tttgcctttc ttccgctaag attgtttcgg
     1921 ggatgatacc tgatttaaag tgattttatt atgatagaga atgtcagaaa atacccggtg
     1981 atcatgatcg cagtgatatc gttctactac atggtcggta tcattggtct ttcacttgaa
     2041 accacgcggg aattgttccg ggcgcttgtc ccgttcaccc tgttgttcag cctctatttt
     2101 ctctggctct ttcatgaaaa tcccggcctt aggttttacc tggtctcgct ggtgatcttt
     2161 ttacttgggt tttttattga agtggtcggt gtaaagacgg gctggttttt cgggcactat
     2221 gtttacggcg aaacactcgg attcaaaatc ctctcaacgc cccttatgat cggtgtaaac
     2281 tggcttttcc tgatcatttc ctgctggtcg ctcaccggcc tgttcctgcg gaaccgctgg
     2341 cttcgctata agacaggggc actattcatg gtgatttacg atattttcct ggaaccggtt
     2401 gcggtcaggc tcgacatgtg gagctggcac ggagataaaa tcccgctgca gaattatata
     2461 ggctggtaca tcacttcctg gttgctattc atcatcctgg accagagcag ggtcagcgtg
     2521 aaaaacaaaa tcgccccttc tttgtttatc atccagtttt tatttttcgt catcttaaac
     2581 atcatcaatt tcttatcctg aatgggagtt ataatcgctc tgacaatcct ggttgcgtgg
     2641 gcagcccacc tggcttacat ccttgccggc ctggagattg actatgcctc cccctggttt
     2701 tattttcata ttttgctcca ggcctatctt tataccgggc tgttcattac ggggcatgat
     2761 gcaatgcacg gggtggtctc ctccaaccgc ccggttaatc aggcaatcgg ttacacagtg
     2821 gtatttcttt atgccggcat gtggtaccgg aaactcatcc gttatcacgg gatgcaccac
     2881 cagtttcccg gtaccgaaag agatcccgat tacagtacgg gatcacagaa cttttttgtc
     2941 tggtggttcc gttttatgat ccattatata acctggatac aattgctgat catggccgca
     3001 ctatacaaca ttctcacgat ctggctgagc gaccggcagg tgatattatt ctgggtcctt
     3061 ccggctgttc tgagcacgtt tcagttgttt tacttcggga cgtatgtgcc tcataaaagg
     3121 ccgcataccg gtcagatggg cccgcataag gccaggacac tcgcgaaatc acatctcctg
     3181 gccatgataa cctgctactt cttcggatac caccgggaac atcatgaatc gccccgaaca
     3241 ccctggtggc agctatacag gttaaaatcc tgaatccatg atcagggccg ggcaccggaa
     3301 attttttgtc atcttcttcg acttttatac gaaatggatg cttcgccgac attttcagcg
     3361 gataatcatt tatacttccc cggatgagac cgataagcct gtcatgatga tcggcaacca
     3421 tttcagttgg tgggacgggt ttatcgccaa ctatatcaat ctggagcttt tccgcagaaa
     3481 gattcacatc atgatgctgg aagaacaact gcgcggccgg atgttcctga ataaagccgg
     3541 cgctttttcc atccggaaga acagccggtc ggccatcgaa agcctcgact ataccgttgg
     3601 gcttctggag aaccgggatc atttggttgt tttatacccc cagggagaat tccagtcggt
     3661 ctacagttcc cggatccgtt ttcagccagg cattatgcgt gttctgaagg gattgcgaaa
     3721 accggttcag atggtttttt atgccgcact caccgactac ttttcatcat tcaggccgca
     3781 tttgtcgatt tatctggaaa cggttgaccc tgatgatctt caggatataa aggccctgga
     3841 gaatgcctac aacgattttt acaacgcctg taaggaaaag caaatgaagg aatgatggaa
     3901 tatttggcct acgcagcggt tgcctatctt gccttacggt tgctggtgat cctggtcaat
     3961 cagttctcag gcactgaaat gaccataagt cccggatcat cgtccaaaac cgtttcggcg
     4021 ctcatccctg cccggaacga ggaagagaac attggcaggc tgctggatga cctttctgcc
     4081 tccgagtatc ctcatctcag gatctttgtt tatgatgatg aaagtgaaga tagaacagcg
     4141 gatattgtta ttgaaaaaag ccggatggat gaccggatca tcctgatcag gggaagaatt
     4201 ccggggcccg gctggctcgg aaaaaatcat ggctgttccc ggctggcaga agcagcagta
     4261 ggagattatt ttttgtttat tgatgccgat gttcgtgtca gtaaaacgct gatcaacagt
     4321 gccgtacaaa gtatggaaca acatcagctg gctttgttgt cgatatttcc ggtgcagatg
     4381 atgaatagta ccggcgaatg gttaacggtg ccgctggtga acaggatatt gg
//
LOCUS       DAOQXS010000240         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20328_length_4394_cov_4.263655, whole genome shotgun sequence.
ACCESSION   DAOQXS010000240 DAOQXS010000000
VERSION     DAOQXS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20328_length_4394_cov_4.263655"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1265
                     /locus_tag="PKM34_07430"
     CDS             69..1265
                     /locus_tag="PKM34_07430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ83457.1"
                     /translation="MTSKASVILVPGRESSVLRRHPWIFSGAVRNVQGNPSAGETVEI
                     YSSAGQWLARGSYSPGSQIRIRILTFDAGETVDETFFENRIRRAVELRQSLPGFREGN
                     CARLIFSESDSLPGLIVDKYDDFLVCQFLSAGAEFWKDIITPKLQEIIPCRGIFERSD
                     SDSRAKEGFAERTGLISGEEPPARVAVDDGKMKFWVDIRQGHKTGFYLDQRLNRLKIM
                     DYANGREVLNAFSYTGGFGVAAAIAGASTVVNVDSSKDALELATENFRLNGLALEKSD
                     FAEDDVFQLLREYRDRRRQFDMIVLDPPKFVASASQLDKGTRGYKDINLLAFKLLRPG
                     GILVTFSCSGYVKPDLFAKIVSDAALDAGRDAVIVENLHQSPDHPVALNFPEGLYLKG
                     LICRVS"
     gene            complement(1285..2121)
                     /locus_tag="PKM34_07435"
     CDS             complement(1285..2121)
                     /locus_tag="PKM34_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83458.1"
                     /translation="MHSDHLPGNGKIFRNLLFRISSYTLTAYFLSFIAEKLLVITGAF
                     LFDFKITWLYNKLIIKGADSNWTQENVLIIYLMPLVIMAIIIVLIYLPKKEKDPQPHY
                     KDLFLTWFAFFLVYRILGMTPFNLVVRTNVFFVFDWLYAGPLVLFSAGILSFILYLLI
                     TNKLLKDYYLHAALLSRNHSRLGNHDFMLASILVPLFITSIAATLFFLPGPPYEELAG
                     LVMMNLPAVMIFIRYKLDPPVLLMTKKETSEMTFASWIFPVCLLTVLAFRIILALNEI
                     IA"
     gene            complement(2129..2542)
                     /gene="mscL"
                     /locus_tag="PKM34_07440"
     CDS             complement(2129..2542)
                     /gene="mscL"
                     /locus_tag="PKM34_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNQ83459.1"
                     /translation="MGLLKEFKEFAMKGNVIDLAVAVIIGGAFGKIVTSFVNDILMPP
                     LGLFLGGVDFKDLKLIIKEGAEGVAAVTWNYGMFIQNIIDFLIIAFAIFMAIRSMTKM
                     QQLRAKPAEPEPEPAPPEPSNEEKLLTEIRDLLKK"
     gene            complement(2625..3722)
                     /gene="corA"
                     /locus_tag="PKM34_07445"
     CDS             complement(2625..3722)
                     /gene="corA"
                     /locus_tag="PKM34_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNQ83460.1"
                     /translation="MKSRKKTNHSPRNKIPLKDTRKSGKTGLPPGSLVHIGKKIADSV
                     TVRHWAFNSSEFSSTAVIDLHELKTSEGVNNWFEICGLHDTTLMDRLGKALNISPLVL
                     EDILNTEHRPKFDIQGGMLFLTLKAFYISGEGEILDEQASFILGSGYLVSFHERSHSW
                     FDSIKAAMEAGNTRIRQRGNDFILYSLLDIIIDRYYAVAESLGDRLEELEELIFENPD
                     RTLIDKNREIKKEIIKLRKALIPSLEAINKLKRGESDLVMKDTLPYFDDIYDHMVQVL
                     DYIDTYREISAELKESYLSNLTLRMNQVMKLLTIITSIFIPLSFIAGIYGMNFRNMPE
                     LGWKYGYYTILGIMFLLTVGMLIYFRRKKWL"
     gene            complement(3763..4158)
                     /locus_tag="PKM34_07450"
     CDS             complement(3763..4158)
                     /locus_tag="PKM34_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83461.1"
                     /translation="MDQKKLEKLENIKNYLENDDISGLNKYIERIKPMVKDDEDFQEI
                     FEELKGKNYDQALFLAEEFIYETDEEADTYGDDFDKVDLEDFDGKNDLMLEGPGDDLE
                     EFDEDLNDDFSEDQYDDLAYYEDKDDDYY"
BASE COUNT         1152 a          951 c         1049 g         1242 t
ORIGIN      
        1 taagacaagg aaaagggaaa aaggaaaagg gaaaaagtac ttttgcagta tgattaaata
       61 ctttggaatt gactagtaaa gcttcagtta tcctggttcc gggccgggaa agttcggttc
      121 tgcggcggca tccctggata ttttccgggg ctgtcaggaa tgttcagggt aatccgtcgg
      181 ccggtgagac cgtggaaata tattcttcgg cagggcagtg gctcgccagg ggaagttact
      241 cgcccgggtc gcagatcagg atccgcatcc ttacttttga cgcgggagag acagtggatg
      301 aaacattttt cgaaaacagg atcaggaggg cagtggaact tcgacaatct ctgccgggat
      361 tccgggaggg caattgtgcc cgtctgattt tcagcgaatc ggatagtttg ccgggattga
      421 tcgtcgacaa gtatgatgac ttcctggtgt gccagtttct atctgccggg gccgagttct
      481 ggaaagatat cataacccct aaacttcagg agattatacc ctgccgggga atctttgagc
      541 gctccgattc cgacagccgg gccaaagagg ggtttgcgga gcgaacaggt ttgatttcag
      601 gggaagaacc tcccgccagg gtagcggttg atgacggtaa gatgaagttt tgggtggata
      661 tccggcaggg gcataagacg ggattttatc tagaccagcg ccttaaccgg ctgaaaatca
      721 tggattatgc caatggcaga gaggttttaa atgctttcag ttatacaggg ggctttggcg
      781 tggctgctgc tattgccggc gcttccacgg tagttaatgt cgattcgtcg aaagatgcac
      841 tggaactggc aaccgagaat ttccggctga acgggcttgc cttggaaaag tcggacttcg
      901 ccgaggacga tgttttccag ctattgcgcg aataccgcga ccggcggcgg caatttgata
      961 tgatcgtcct ggatccgccc aagtttgtgg cgtctgccag ccagctggac aagggcacgc
     1021 gcggttataa ggatatcaac ctgttggcat tcaagctgct gcggccaggt ggaatactgg
     1081 ttacattttc ttgctcaggg tatgtaaaac ccgacctttt tgctaagatt gtttccgatg
     1141 cagcccttga cgccggaagg gatgccgtta tcgtggaaaa tctccatcag tcccccgatc
     1201 atccggtagc gcttaatttt cctgaaggcc tttacctgaa aggattgatc tgccgcgtaa
     1261 gttgaaaatt taacggtcag attatcaggc tattatttca tttaaggcca gaatgatccg
     1321 gaaggccagc accgttagca ggcatacggg aaatatccat gatgcgaacg tcatttcact
     1381 ggtttccttt tttgtcatta ataatacagg cgggtcgagt ttatagcgga tgaagatcat
     1441 gacagcgggg agattcatca tgaccagccc ggcaagttct tcatacgggg gcccgggaag
     1501 gaaaaaaaga gtggcggcaa tggatgtgat gaaaagggga accaaaatgg aggccagcat
     1561 gaagtcgtga ttgccgagcc ttgaatgatt ccggctcaat aaagctgcgt gtaagtaata
     1621 gtctttcagc aacttgttag ttataagcaa atagagtata aatgacagta tgccggcgct
     1681 gaacagcacc agcggcccgg catacagcca atcgaataca aaaaagacat tggttcggac
     1741 gaccaggttg aaaggggtca tccccaggat tctataaacc agaaagaatg caaaccaggt
     1801 caggaataaa tctttgtaat ggggctgagg gtctttttcc tttttcggaa gatatatcaa
     1861 tacaattatt atagccatga taaccaacgg catcaggtag attatcagga cattctcctg
     1921 ggtccagttg gaatcggccc ctttgattat tagtttgttg tacagccagg tgattttgaa
     1981 atcaaacaga aaagctcccg tgataaccag gagcttttct gcgataaatg ataaaaagta
     2041 agcagttagt gtatacgaag aaatcctgaa aaggaggttc ctgaaaattt tcccgttccc
     2101 cggcaaatga tcggaatgca taggaaaatt attttttaag caggtccctg atttctgtga
     2161 ggagtttttc ttcgttagat ggttcgggtg gcgccggttc cggttccggt tcagcgggtt
     2221 tggccctgag ttgctgcatt ttggtcatcg accggatcgc catgaagatc gcaaatgcaa
     2281 tgatcaggaa atcgatgatg ttctggataa acatcccgta attccaggtc accgctgcta
     2341 ctccttcggc tccttccttt ataataagct tcaggtcttt gaaatcaacc cctcccagga
     2401 acaatcccag gggcggcata aggatgtcgt tgacaaaaga ggtgacaatc tttccgaatg
     2461 cgccgccaat gatcacggct acagcaaggt cgatcacgtt gcctttcatg gcaaattcct
     2521 tgaattcttt aagcagtccc atattctttt tggtttaagt gaaacaataa tttaaaagat
     2581 ataaaaattc aaaatgacta taaagatatt taaatgttaa agaatcataa ccattttttt
     2641 cgccggaaat aaatgagcat tccgacggtt aacaaaaaca ttatgcccag aatagtatag
     2701 tacccgtatt tccagcccag ttcaggcata ttcctgaaat tcattccgta tatccccgcg
     2761 ataaagctta acgggataaa gatcgaagtg atgatcgtaa gcaacttcat gacctggttc
     2821 atccgaagtg tcagattcga caggtagctt tcttttagtt cggcgctgat ttcccggtaa
     2881 gtatcgatat agtccagcac ctggaccata tggtcgtaaa tatcgtcaaa atagggaagg
     2941 gtatctttca tcaccaggtc ggattcgcct ctcttcagtt tgttaatggc ttccagtgaa
     3001 gggatcagcg cctttcgcaa tttgatgatc tcttttttga tctccctgtt tttatcgatc
     3061 agggtccggt ccggattctc aaagatcagt tcttcaagct cttccaggcg gtctcccaat
     3121 gattcggcaa cagcgtaata ccggtcgata atgatatcga ggagggaata aaggataaaa
     3181 tcgtttcccc gctgccggat tctggtattg cctgcttcca tcgctgcctt gatggaatcg
     3241 aaccaggaat ggctcctctc atgaaatgaa acgaggtatc cgctgccgag gataaaactg
     3301 gcctgctcat ccaggatctc cccttcgccg gaaatgtaaa aagctttcag ggtcaggaaa
     3361 agcatgccgc cctgaatatc gaatttgggg cggtgttcgg tatttaatat gtcttcaaga
     3421 accagagggc tgatattgag tgctttgcca agacggtcca tcagggttgt gtcgtgcaac
     3481 ccgcatatct caaaccaatt gtttaccccc tccgatgttt tcagttcatg gagatcgatt
     3541 acggcagtac tggaaaattc ggaggagttg aaagcccagt gcctcaccgt tacagaatcg
     3601 gcaatctttt tgccgatatg tacgagcgaa ccgggcggta atccggtctt tcctgacttc
     3661 ctggtatctt ttaacggaat tttattcctg ggggagtggt tcgttttttt tcgggatttc
     3721 atattcccga aattacgatt attttagaat ccaattattc cattaataat aatcatcatc
     3781 tttatcttca taataggcca ggtcatcgta ctgatcttcc gaaaaatcat cgttcagatc
     3841 ttcatcgaat tcttccaggt cgtctcccgg gccctccagc atcaaatcgt tcttgccgtc
     3901 aaaatcttca agatcaacct tatcgaaatc atcgccgtaa gtatctgctt cttcgtcggt
     3961 ttcataaatg aactcttcgg ccaggaacaa cgcctggtca tagttcttcc ctttcagttc
     4021 ttcaaaaatt tcctgaaagt cttcatcgtc cttgaccatc ggcttgatcc tttcgatata
     4081 cttgttcaac cctgaaatat catcattttc aagataattc ttgatgtttt ccagtttctc
     4141 gagcttcttc tggtccattt taggtattat tggttttaaa aagcggatgc aaatatattt
     4201 ttcctttaat tatctgcgac atggtgaaat aaaattttct ttccaaagtt taacattcag
     4261 gtcgttgatc atttgatcgc atcagaccaa cttaataaat gagaatgatt atttttgcgc
     4321 tttccctgat gaaatgagtt ttttacatcc atatatgctt tggggattac tggcggtttc
     4381 catcccggtt atca
//
LOCUS       DAOQXS010000241         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20478_length_4371_cov_3.231001, whole genome shotgun sequence.
ACCESSION   DAOQXS010000241 DAOQXS010000000
VERSION     DAOQXS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20478_length_4371_cov_3.231001"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="trxA"
                     /locus_tag="PKM34_07455"
     CDS             <1..343
                     /gene="trxA"
                     /locus_tag="PKM34_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ83462.1"
                     /translation="FLNKVFDYEKNKEWNFAGELPCLIDFYADWCGPCKMVAPILEEL
                     AEEYKGKINIYKVDTEEQQELAGVFGIRSIPSLLFCPKDGQPQMAMGALPKDTFRKAI
                     DDILLKAEVKN"
     gene            347..979
                     /locus_tag="PKM34_07460"
     CDS             347..979
                     /locus_tag="PKM34_07460"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNQ83463.1"
                     /translation="METPRNVLLPTAYFPPVSYFASLFSSNHALIEACETFPKQTYRN
                     RCEILTSSGRQNLVVPVSKPYGNHTLTRDVLISYREPWHQHHWKSITTAYRSSPYFYY
                     YADLLHPLFNRMESSLLIHNQNILIALIKILGIRPEISLTIDYSKIADGCNDLRTKMK
                     PGRKEPGIFPEYPQVFSHLTGFIPDLSILDLIFNLGPASGDYLNKVVITP"
     gene            complement(969..1748)
                     /locus_tag="PKM34_07465"
     CDS             complement(969..1748)
                     /locus_tag="PKM34_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNQ83464.1"
                     /translation="MIQFGFCELAVIPVRSEPSDKAEMSTQMLFGDIFKVKEIAGPWL
                     FITSFYDGYEGWIDQKQARMISGEEYKRLSSAPLFVNRRIQGDSVKLEGQLVLLPAGC
                     SFYDVKGRVMAVEDQNYVVEGNIFPFRFDNTGSLMETAFSYLSAPYLWGGRTCFGLDC
                     SGFTQIVFKQHGIRLFRDASQQASQGELIPFLSDGMPGDLAFFDNEEGTITHTGILLD
                     NQKIIHCSARVRIDDIDHQGIFNREKMTYTHKLRLIRRVTG"
     gene            1813..3897
                     /locus_tag="PKM34_07470"
     CDS             1813..3897
                     /locus_tag="PKM34_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ83465.1"
                     /translation="MDNPKQNNQNRRELLIKIGLFVFAVVLLVIVLPKEGKFRYEFAK
                     GKPWLHDDLYAPFDFAIIKFDEEIKAEQEQTLKNLRPYFTLDTLVASSQAQLFINTFE
                     NGWKERYGSGNALYQQKEHLINTGLAILDSLFGKGIIELNASIENKPAGFEVYILRKN
                     EAREAPLHKVYTLQQATSFISMKLNRLGGEDTETLRNALVNSITYNLFFDKEKTEAEK
                     NSALESISPFRGMVQRGELIIPRGARVNDDHFRILTSLKNNYEVHLGAAKARYAITGG
                     QAILTSISILVLILFLYSYRKDILANNKRIALILLLIFSMVFIASMVVRYEVSYFYLV
                     PVCLVPVIIRVFYDTRLALFVHLITIFITGFIAPNSFQFVFIQLIAGVVAIISIVRLE
                     RRSQFFYTAFWVFLTYVAIYVGLNLMQEGNFHSIHIRHIYQMAGNAGLLLFAYPLMFL
                     IERSFNYITDVTLIELGNSNNKLLRELARKAPGTFQHSLQVANLAEEASFAIGGDSKL
                     VRVGALYHDIGKIEAPLYFIENQVSGINPHDQISHEESARIIIRHVTRGVEIAKKHKL
                     PPEVIDFITTHHGTRKVEYFYIRQKLENPQEGLDEEAFTYPGPIPFSKETALVMMADS
                     VEAASRSLTNPDDKAIDELVERMIGRQLDSGQLAEANLTLRDIAQLKTIFKRNLQNIY
                     HVRISYPEEGGK"
BASE COUNT         1185 a         1084 c         1022 g         1080 t
ORIGIN      
        1 ttttctgaac aaggtatttg attacgaaaa aaacaaggaa tggaattttg ccggagagct
       61 gccgtgcctg atcgattttt acgccgactg gtgcggaccg tgcaagatgg tagccccgat
      121 actggaggaa ctggccgagg agtacaaagg gaagatcaat atatataagg tagacacgga
      181 agagcagcag gaactggccg gcgttttcgg tatccgcagt attccttcgc tgttgttctg
      241 tccgaaagac ggccagccgc agatggccat gggcgccctg ccgaaagaca ccttccggaa
      301 agccatcgac gacattctcc tgaaagcgga agtaaaaaat taagctgtgg aaaccccgag
      361 aaatgtgctt cttcctacag cgtatttccc gccggtctct tattttgcca gccttttcag
      421 cagcaaccat gctctgatag aggcctgtga aacctttcca aaacaaacct accggaaccg
      481 atgtgagatc ctgacctcct cgggcaggca gaacctggta gttccggtat caaagcctta
      541 cggaaatcat acattaaccc gggatgtttt gatctcttac cgggaaccct ggcaccagca
      601 tcactggaaa tccattacta ccgcataccg ctcatcgcct tatttttact actacgccga
      661 cctgctgcac ccgctcttca accggatgga gagctcgctt cttatccata atcaaaatat
      721 tcttattgct ttaattaaaa tactggggat aagaccggag ataagcctga ccattgatta
      781 ctctaagata gcagatggtt gcaatgatct tcggaccaaa atgaagccgg gcaggaaaga
      841 acccggaatc tttcccgaat acccccaggt tttcagtcat ctgacaggat ttatccctga
      901 cctgagcata ctggacctga ttttcaatct cggccctgct tccggagatt atttaaacaa
      961 agtggttatt accccgtgac ccgcctgatc agcctcagct tgtgcgtgta ggtcattttt
     1021 tcacggttga agataccctg gtgatcgata tcatcgatcc ttaccctggc gctacaatga
     1081 atgatcttct ggttatcgag caggatgccc gtgtgagtga tagtgccctc ttcgttgtcg
     1141 aaaaaggcga gatcgcccgg catgccgtcg ctcagaaagg gaatgagttc gccctgggat
     1201 gcctgctgag aagcatcccg gaaaagccgg atgccgtgct gtttaaagac gatctgggta
     1261 aaacccgaac aatcgaggcc aaaacaagtc ctgcctcccc agagataagg cgccgataga
     1321 tagctgaagg ccgtttccat taagctgcca gtgttgtcaa accggaaagg gaatatattt
     1381 ccttccacga cataattctg gtcttcgacg gccatcaccc gtcctttaac atcataaaaa
     1441 ctgcatccgg ccggcaataa aacaagctgt ccttccaatt ttaccgaatc accctgaatt
     1501 ctcctgttta caaataatgg ggctgaggat agccttttat attcttcccc gctgatcatc
     1561 cgggcctgtt tctggtcgat ccatccttcg taaccgtcat aaaaactggt tataaaaagc
     1621 cagggaccag ctatttcttt cactttaaat atatcgccaa acagcatctg ggtcgacatt
     1681 tccgctttat cagaaggttc ggatctgaca ggaataacgg cgagttcaca aaaaccgaat
     1741 tgtatcataa aaatatgatt tcgtgcaaat ttaaacctaa aatctctaaa ttagcagcag
     1801 gaagtaagca tcatggacaa tccaaagcaa aataatcaaa accgccgcga gttactgatc
     1861 aaaatcggtt tattcgtttt tgccgtggta ttgctggtga tcgtactacc caaggaggga
     1921 aagttccgct acgagtttgc caaagggaaa ccctggctgc acgacgattt gtatgcgcct
     1981 tttgattttg ccattatcaa gttcgacgaa gagatcaaag cggaacagga gcagacatta
     2041 aagaatctca ggccatattt taccctcgac acgcttgtgg cttcctccca ggcccaattg
     2101 ttcatcaata cgtttgaaaa cggatggaaa gagaggtatg ggagcggaaa tgccttgtat
     2161 cagcagaaag aacacctgat taacaccggg ttagcgatcc tggattcgtt attcggcaag
     2221 ggcattatcg aactgaatgc ttcgattgaa aacaaaccgg ccggtttcga ggtttacatc
     2281 ctgagaaaga acgaagcccg ggaagcacct ttgcataagg tatacacttt acagcaagcc
     2341 accagtttca tttccatgaa attaaaccgc ctcggcggcg aggacacaga gacgctccgg
     2401 aatgccctgg tcaattccat tacctacaac ctgttcttcg acaaggagaa gaccgaggct
     2461 gagaaaaact cagcgctcga aagcatcagt ccgttcagag gcatggtcca acggggcgaa
     2521 ttgatcattc cccggggggc gcgggtcaac gacgatcact tccggatcct gacatcgctc
     2581 aaaaacaatt acgaggtaca cctgggtgct gccaaagccc ggtacgccat tacgggcgga
     2641 caagctatcc tgacctccat atccatcctc gtcctgatac tttttctcta tagttaccgg
     2701 aaggatatcc tggccaacaa caagcgtatt gccctcatcc tgttgcttat cttttccatg
     2761 gtatttatcg ccagcatggt cgtcaggtat gaagtgagct acttctacct ggtaccggta
     2821 tgcctggtac cggtgattat ccgggtgttt tacgacacac gcctggcgct tttcgtccac
     2881 ctcatcacca tctttatcac cggcttcatt gccccgaaca gtttccagtt cgtatttatc
     2941 cagttgatag ccggggtggt ggcgatcatc agcattgtca ggctggaaag gcggagccag
     3001 tttttttaca cggcattctg ggtgtttctg acttatgttg cgatctatgt cgggttgaac
     3061 ctgatgcagg agggcaactt ccatagcatt cacatccggc atatttacca gatggcaggc
     3121 aatgccggat tgctgctatt tgcttacccg ctgatgttcc tgatcgagcg gtcgttcaat
     3181 tatattaccg acgtcacgct gatcgaactg ggcaacagca acaacaaact tctccgggaa
     3241 ctggcccgga aagcgccggg gacattccag cactcgctgc aggtggctaa cctggccgaa
     3301 gaggcatcgt ttgccatcgg cggcgacagc aagctggtgc gggtcggcgc cctctaccac
     3361 gatatcggga aaatagaggc gccgctgtat ttcattgaga accaggtgtc agggataaat
     3421 ccacacgacc agatcagcca tgaagagagt gcgcggatca tcatccgcca tgtgacccgc
     3481 ggcgtggaaa tcgcaaaaaa gcacaaatta ccgccggagg tgatcgattt catcacaacc
     3541 catcacggaa cccggaaagt agagtatttc tatatcaggc aaaaactgga aaatccacag
     3601 gaggggctgg atgaagaggc ctttacctat ccgggcccga tcccgttttc caaagagacc
     3661 gccctggtga tgatggccga ttcggtggag gccgcatccc gaagcctgac caacccggat
     3721 gataaagcca tcgacgagct ggtggaaagg atgatcggcc gccagctcga ttcggggcag
     3781 cttgctgaag ccaatctgac cctgcgcgac atcgcccagt taaaaacgat cttcaaaagg
     3841 aacttacaga atatttacca cgtcaggatc agctacccgg aggaaggcgg gaaatagttc
     3901 cgggtttcag gttccgggtt ccaggttccg ggtttcaggt tccgggtttc tggttcattt
     3961 gtatattata ttttaatcac tccgaaagat attaataaga tcttactaca taagatcagc
     4021 ttgtcccgag acctcgggat agggcatatg gatcttcaaa tagttgatta tgaggccaaa
     4081 ctctatgtga attcctatgt gccctatgtt cctatgtggt taataaataa cttttaaaag
     4141 caaattcaaa ttaaaaatcc aaacttattc aaatataaag taacaaaatt gatttttcgg
     4201 gacaacaaat ataatttatg ccgatttgat gaaaagtcct tgacattagc cggatttgaa
     4261 ctatattcac agcgttaatc ataccgagtc tgaaccggtt tgcggattta atacagtcgt
     4321 tcctctcggc atagaggctg tatcaaaagt aataaaccac aaaggaacac c
//
LOCUS       DAOQXS010000242         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20578_length_4355_cov_3.853023, whole genome shotgun sequence.
ACCESSION   DAOQXS010000242 DAOQXS010000000
VERSION     DAOQXS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20578_length_4355_cov_3.853023"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PKM34_07475"
     CDS             <1..1378
                     /locus_tag="PKM34_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83466.1"
                     /translation="IHKIRLDYLALLIILSGLVYTNLYYKFWKQPGRIIAHDVILYYQ
                     YLPAAVIYKDLSLKFTSENPDFFKDKIWKIHTESGNGVSKMTMGLAVLYSPFFLAAHA
                     FAGKLGYPADGYSDPYRFMLILSSLVYAAFGFIFLIKLLNKYFARSTVALTILAIGLG
                     TNLYFYTTIEPTMSHSYSFFLFAAFLFLVDAWIQKQSWVNTVFLGIVGGLIILVRPTN
                     GIVFILLLLWKIDSWRGLVDRIRLFLSLYLKTGAIAFLVILLFLPQVIYWKILTGDFF
                     FYSYRDEKFFFNDPAFLHGMFSYRKGWLVYTPMMIFSILGMIVMYFKHKRLFWPVLTF
                     TFLNLYIIFSWWCWWYGGGFGQRALIESYAVLAVPFAAFTDFILNRKLVLKIIFLTLV
                     TGFIALNIFQTRQYYKGVIHWDAMSKYTYWDSFFRTKVAPNFYNQVEKPDYKAAMKGD
                     RYEIMKQN"
     gene            1385..2251
                     /locus_tag="PKM34_07480"
     CDS             1385..2251
                     /locus_tag="PKM34_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83467.1"
                     /translation="MLNNPDITASIIIPVYNEDKGIGILLEKMTGMKLHETFEIIVVN
                     DGSTDNSLEVIRQYPVKVYTHTVNKGYGAALKTGIRKAGGAKVIILDSDGQHDPAQIR
                     EIAGMLDEHEMVIGTRNKDSFQVKTRQVGKKVIRWVGEYLVEQKLPDYNSGYRGFQRE
                     LIHSMLHMMPNGFSFSTTSTLAFLKEGYSIGTIPIKVEERVGRSSNVKFFKDGAKTMM
                     LILRIIMLFNPLKIFFPASFIFTTMGVLYGIYGYIIAERFSNGAIVMVILGMFLFFIG
                     LVADQISIMNRR"
     gene            2248..2967
                     /locus_tag="PKM34_07485"
     CDS             2248..2967
                     /locus_tag="PKM34_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ83468.1"
                     /translation="MIRKNTWTDKEVEAHWDNVADIYVAENNKVKDTHDQRFRETVHH
                     LRLEPGMKVLNITSRDAEADDYIRRANRDVEVINAEISSGLIDVAAKIRPAVKQVKIG
                     NYSILPFQDAEFDRVISLETLEHVAEPLRFLSELHRVSNPDARLVLSCPPATSELPYR
                     IYTALFGGHGEGPHRFLPSKEVKEMFAETKWKPVLHKGTILIPVGPSWLRNFGENIIG
                     RFQGTFIAELGIRQFFVCEKA"
     gene            2954..4267
                     /locus_tag="PKM34_07490"
     CDS             2954..4267
                     /locus_tag="PKM34_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ83469.1"
                     /translation="MKKPENIQDVIRHDLCNRCGSCVGLSGGKIVFENRTGSYLPVIK
                     EKPDKATEELIWQACPGKGFDFPEQRNAIYGEQEAHPYIGHYREIYIGFAADPETRLS
                     AASGGIISAILIWLLETGRIKGAVVLAMSEQEPWLTKPVIATTKEQILEAAQSKYIIS
                     SVNEILPEMERFDGPLAYVGLPGQVQSIRLLRQISHPSVRNIRYIFGPFYGNTLHFSS
                     VKSFLRSFKVKDYTLIRKLYFRHGEWPGKMRVELSDGRAFELPKFHANYLIPFHILKN
                     SLLCTDLTNEYTDISGGDAWAPVYEERGKGFSMVISRSEEGGKILREMADAGRLELSP
                     ITEKEAVDMHSHGYDLKKRGTFIRMKYRSILGKPNPDYGYIIKGFSVSRYLMEFIINV
                     LFLMAGTRFARWTVERIPPETIGKIFEKARKFWKKSTHNIKREKL"
BASE COUNT         1115 a         1037 c         1095 g         1108 t
ORIGIN      
        1 gatacataaa ataaggcttg attacctggc ccttctcatt atcctttccg ggctggttta
       61 taccaattta tattacaaat tctggaaaca acccggcagg attattgccc atgatgtcat
      121 cctgtattac cagtatctgc ctgccgctgt tatatataaa gacctgagtc tgaagttcac
      181 atctgaaaat ccggactttt ttaaggacaa gatctggaaa attcacaccg aatcggggaa
      241 tggggtaagc aaaatgacga tgggactggc tgttctgtat tccccgtttt tcctggcagc
      301 tcacgcattt gccggaaaat tgggatatcc tgccgacggc tactctgatc cttaccgttt
      361 catgctgatc ctttccagcc tggtatatgc cgcattcgga tttatttttc tgataaagct
      421 actgaataaa tattttgccc gaagcacggt agctcttacc atcctggcga tcggcctggg
      481 taccaacctc tatttttata cgacgattga gccaaccatg tctcattcct atagcttctt
      541 cctttttgcc gctttccttt tcctggtgga tgcgtggata cagaagcagt catgggttaa
      601 cacggtcttc ctcgggattg tcgggggatt gatcatcctg gtcaggccta caaacggtat
      661 tgtttttatc ctccttttat tatggaaaat cgatagctgg agaggactgg ttgacagaat
      721 aagattattt ctgagccttt acctgaaaac cggtgcaatc gctttcctgg tcattttatt
      781 atttcttcct caggtcatct actggaaaat ccttacgggc gattttttct tttacagcta
      841 cagggatgaa aagtttttct tcaatgatcc cgctttttta cacggcatgt tttcgtaccg
      901 gaaaggatgg ctggtttaca ccccgatgat gattttttcg atactgggga tgatcgtcat
      961 gtatttcaaa cataagagat tattctggcc tgttttgacc ttcacttttc tgaaccttta
     1021 cattattttc tcctggtggt gctggtggta tggcggggga ttcggccaac gggccctgat
     1081 cgaatcttat gccgtgctgg ccgttccttt tgccgctttt acggatttta tcctgaacag
     1141 aaaactggta ttgaaaatca tatttttaac cctggtaacc ggttttattg cactgaacat
     1201 attccagacc cggcagtact ataaaggtgt aattcactgg gatgcaatga gtaagtacac
     1261 atactgggat tccttcttcc ggacgaaagt cgccccgaat ttttataatc aggttgaaaa
     1321 gcccgattat aaggcggcca tgaaaggtga ccggtatgaa attatgaaac aaaactaaac
     1381 tccaatgcta aacaatcccg atataacggc ttccatcatc atccctgtct ataacgagga
     1441 caaggggatc ggcatccttc tggaaaagat gaccggcatg aagctccacg aaacctttga
     1501 gatcatcgtt gtcaatgatg gctctaccga caattcgttg gaggtgatcc ggcaatatcc
     1561 ggtcaaagtt tacacccata ccgttaacaa gggttatggc gcagcgttga aaaccggcat
     1621 ccggaaagcc ggcggcgcca aggtcatcat ccttgacagc gacgggcagc acgatccggc
     1681 ccagatccgg gaaattgccg gcatgctgga tgagcatgaa atggtgatcg gaacgcggaa
     1741 caaagattcc ttccaggtga aaacgcggca ggtggggaaa aaagtgatcc gctgggtcgg
     1801 cgaatacctg gtcgaacaaa aactgccgga ctacaattcg ggataccggg gatttcaaag
     1861 agagctgatc cacagcatgc tccacatgat gccgaacgga ttttcctttt caaccacatc
     1921 tacactcgca tttctgaagg aagggtattc catcggaacg atccccatta aggtggaaga
     1981 gcgtgtcggc cggtcgagca atgtcaaatt ctttaaagat ggcgctaaga ccatgatgct
     2041 catcctccgg atcatcatgt tgttcaatcc gctgaaaata tttttcccgg caagctttat
     2101 attcacaaca atgggcgtgt tgtacggaat ttacgggtat atcatcgccg agcgtttctc
     2161 taacggggcc attgtcatgg tcatcctggg gatgttcctg ttttttatcg gcctggtggc
     2221 cgaccagata tcgatcatga atcgccgatg atcaggaaga atacatggac cgacaaagaa
     2281 gttgaagcgc attgggacaa tgtggctgat atctatgttg ctgaaaataa taaagttaaa
     2341 gatacgcacg atcagcgttt ccgcgaaacg gtccatcatc tccgcctgga accggggatg
     2401 aaagttttga atatcaccag ccgcgatgcg gaggccgatg attacatccg gcgggcaaac
     2461 cgcgatgtgg aagtgattaa cgccgagatc tcgagcggcc tcatcgatgt ggctgcgaaa
     2521 atcagaccgg cagtcaagca ggttaaaatc ggcaactact ccatcttacc ttttcaggat
     2581 gctgagttcg atcgcgttat cagcctggaa acgctggaac acgtggcaga gccgctccgt
     2641 ttcctgtcgg agcttcaccg ggtttccaac cccgatgcac gattggtatt aagctgcccg
     2701 ccggctacga gcgaactgcc atacaggatc tatacagcgc tgttcggcgg gcacggcgaa
     2761 ggcccgcacc gctttctgcc atcgaaagaa gtaaaagaga tgttcgcgga aacaaaatgg
     2821 aagccggtgc tccataaagg caccatcctg attccggtcg gcccgtcgtg gctgcgaaat
     2881 ttcggtgaga atatcatcgg caggttccag ggtactttca ttgccgagtt gggaataaga
     2941 cagttttttg tgtgtgaaaa agcctgaaaa catacaggat gtaatacgcc acgacctgtg
     3001 caaccggtgt ggctcctgcg tgggattatc aggcgggaag atcgtttttg aaaaccggac
     3061 aggaagttat ttgccggtta ttaaagaaaa accggataaa gcgacggaag aactgatctg
     3121 gcaggcctgt ccggggaaag ggttcgattt cccggaacaa aggaatgcga tttacggaga
     3181 gcaggaagcg catccctaca tcggccatta ccgggaaatc tacatcggtt ttgctgccga
     3241 tccggaaacc cggcttagcg cggccagcgg cgggatcatc tccgccatcc tgatctggct
     3301 gcttgaaacc ggcaggatca aaggcgccgt cgtgctggcc atgtcggagc aggagccctg
     3361 gctgaccaaa ccggtcattg ccacaaccaa agaacaaatc ctcgaagctg cgcaaagcaa
     3421 atatatcatc agttcggtca acgaaattct ccccgaaatg gaaagattcg acggtccgct
     3481 ggcctatgtc ggcctgccgg gacaggtgca atcgatccgt cttctccggc agatcagcca
     3541 tccgtcggtc aggaacatcc ggtacatttt cggcccgttc tacggtaata cactccactt
     3601 ctcctcggta aaaagttttc ttcgatcctt taaagtgaag gattatacat tgatccgcaa
     3661 gctctacttc aggcacggcg aatggcccgg gaagatgagg gtggaactca gcgacggaag
     3721 ggcctttgag ttgccgaaat ttcacgccaa ctacctgata ccttttcata ttctgaagaa
     3781 cagcctgtta tgcaccgatc tgaccaatga atataccgat atatcgggag gagacgcctg
     3841 ggcgccggtc tatgaagagc ggggcaaagg gttttcgatg gtgatcagcc ggtcggaaga
     3901 agggggaaag atcctcaggg agatggcaga tgccggccgg ctcgagctca gcccgatcac
     3961 agaaaaggag gcggtcgaca tgcactctca tggctacgac ctgaagaaaa gaggtacctt
     4021 tatccggatg aaataccgtt ccatcctggg aaaaccgaat cccgattacg gctacatcat
     4081 caaaggtttt tcggtcagcc gttacctgat ggagttcatt atcaatgtat tgtttctgat
     4141 ggcaggaaca cgttttgccc ggtggaccgt cgagcggatt cctccggaga ccattgggaa
     4201 gatctttgaa aaagcaagga agttctggaa gaaatccacg cataatatta aacgggagaa
     4261 gttgtgagcg atggctgtta aaaaattcaa ctggtttacc attctccgcc tggcgggtgt
     4321 ggcccttttt atcgtcgtcc tggccaggac cgacc
//
LOCUS       DAOQXS010000243         4352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20593_length_4352_cov_3.827554, whole genome shotgun sequence.
ACCESSION   DAOQXS010000243 DAOQXS010000000
VERSION     DAOQXS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20593_length_4352_cov_3.827554"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKM34_07495"
     CDS             <1..809
                     /locus_tag="PKM34_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83470.1"
                     /translation="MIIRPYKTLLIRLNGRYTIGDFNNTGFVLQGLANQHYRDKKGRS
                     GDISFSFLTTSQQAGYFYGHFNSNYFRWDTSFSNQIMQLATFSLRYQGLTARVEYQFI
                     SDYVYLNQQALPSQYKGSINLIRAELNEEFRWKVWAFNAQVIYTINSNKDLIRVPDLM
                     ARASFSPTFSLFKNAAVLQPGVEVFYNTAYYASAYMPALRMFYLQDEKKIGNYAYLDV
                     FINLMIKRFRIFVKYQHLNSLWSEHRYYMVPHYPMQEGAFKFGLSWSFYD"
     gene            complement(990..1712)
                     /locus_tag="PKM34_07500"
     CDS             complement(990..1712)
                     /locus_tag="PKM34_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNQ83471.1"
                     /translation="MAKLRFAKWIGGGLGWAWGGPIGGILGFIFGAMIDGMQSGKFEY
                     KPTQSGDFSVSLLILSAAVMRADGKVMKSELEYVRQFFSRQFGQEVANQRIQMLQEIL
                     KQDFNLPEVCRQISHYMDYPAKLQLLHYLFGICAVDNQYNPKEVEMVGTIAGFLGIER
                     QDFESIRAMFVKDIDSAYKILEVNPDATDEEVKAAYRKMAVKYHPDKVEHLGPEIRKS
                     AEEKFQQLQAAYDEIKKRRGIK"
     assembly_gap    2024..2033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2034..3213)
                     /locus_tag="PKM34_07505"
     CDS             complement(<2034..3213)
                     /locus_tag="PKM34_07505"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83472.1"
                     /translation="MKKVLILGAGMVVNPIVRYLLDKNFIVTVATRTKSKADEMIGGH
                     PNGRSVAWTVEQEAELDQMIAEHDLTVSLLPYLYHVMVAKKCIRLKKNMVTTSYVKPE
                     MKALDAEAKAAGIIILNELGLDPGIDHMSAMRIIDHIHGKGGNVDEFYSFCGALVAPE
                     VEENPFHYKFTWAPKGVVMAGNNDGNFLKDGVVKYIPTEQLFRNPLKVDFPKVGLLDV
                     YPNRDSLPYVELYGIPEAKTIMRGTFRYEGWCEIIDALKVLKLITYDKFNMEGMSYAG
                     MMAKMIGESSTENIVAKVAAKLGVKINARAIVAMEWLGLFSNEPMNRMEDSTFEVVSD
                     LMIAKMMIQPEERDMVVMQHTFLASYPDGKQEVIKSRMLDFGTLKTDTSVARTVALPA
                     A"
     gene            complement(3293..>4352)
                     /locus_tag="PKM34_07510"
     CDS             complement(3293..>4352)
                     /locus_tag="PKM34_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83473.1"
                     /translation="KTYIFFSHVIKGQPHNMPMLKRLMELNCTLIDYERVVDEMGKRL
                     IFFGRYAGLAGMINSLWSLGQRLEEFGVITPFLDIAQTHNYHSLDEVRKVVSKVGQKI
                     IEKGLPPELKPLVIGFTGYGNVSQGAQEIASLLPVKEILPDELFNLHKHTHLPDNIIY
                     KVVFKEEHLVKPKNPDHAFDLQDYYQHPENYCSQFEQYIPYLTVLVNCIYWDPRYPRL
                     LTKEFTEQLFKDGKPRLTVIGDITCDPDGSVEITHRGTEIENPVFVYNPSTRKATDGF
                     KGKGLLVMAVDILPSELPRDASRFFSEVLWRYVAPIAKTDFSQPFEKVKLPGPIRKAT
                     ILYKGELTPDYEYLQKYI"
BASE COUNT         1077 a         1026 c         1010 g         1229 t
ORIGIN      
        1 cgatgatcat ccggccgtat aagacattgc ttatcaggct caacggtcgc tacaccatcg
       61 gcgattttaa caacaccgga tttgtgctcc agggattggc caatcagcat taccgggata
      121 aaaaaggaag atccggagat atcagcttca gttttctgac gacaagccag caggcaggat
      181 acttttacgg gcatttcaat tcaaattact tcaggtggga tacgtcattc tcaaaccaga
      241 tcatgcaact ggccacgttt tctctgcgat accagggttt gacggccagg gtcgaatacc
      301 agttcatcag cgattatgta tatctgaacc agcaggcgct gccatcacag tacaaaggga
      361 gcatcaacct gatcagggct gagttaaatg aggaatttcg gtggaaagtg tgggcgttca
      421 atgcgcaggt catttacacg atcaattcca ataaagacct gatcagggtg cctgacctga
      481 tggccagggc ttcattcagt cccactttct ctctttttaa aaatgccgcg gtactacagc
      541 cgggtgttga ggtgttttac aatacggcct attatgccag cgcttatatg ccggcattaa
      601 ggatgtttta tttgcaggat gagaagaaaa taggtaacta tgcctacctg gatgtgttta
      661 ttaacctgat gatcaagcgg ttcaggatct ttgtcaaata ccagcatcta aacagtttat
      721 ggagcgaaca ccgctactat atggttccgc actacccgat gcaggaaggg gcgtttaagt
      781 tcgggctgag ctggagcttc tatgattaaa ttccaagcac caactataaa attccaagca
      841 ccaaatccca aattccaagc aaattcgaaa acttaaaaaa caaattcaaa aaagttttgg
      901 attttgaata ttaaattttg tgcttgtttg ggatttggtg cttggaattt ggaatttgtt
      961 tggaatttgg tgcttggaat ttggaatttc tatttgatcc ctctccgttt cttgatctca
     1021 tcatacgctg cctgtagctg ctggaatttc tcctcggctg atttgcggat ttcggggccc
     1081 aggtgttcga ccttgtcggg atggtattta actgccatct ttctataagc cgctttgacc
     1141 tcctcgtcgg ttgcatcggg attgacctcg aggattttgt aagcgctgtc gatatctttg
     1201 acaaacatgg cgcggatgga ttcaaagtcc tgcctttcaa tccccaggaa acccgcaata
     1261 gtgccaacca tttcgacctc tttcggattg tactggttat ctacggcaca aatcccaaac
     1321 agataatgca gaagctgtag ttttgcaggg taatccatgt aatggctgat ctgccggcaa
     1381 acttccggca ggttaaaatc ctgcttgagg atttcctgga gcatctgaat ccgttgattg
     1441 gccacttcct gtccgaattg ccgggaaaag aactggcgca catattccag ctccgatttc
     1501 atcactttgc catcggcacg cataacggct gccgagagga tcagcaggct gacgctgaag
     1561 tcgcccgact gtgtgggttt gtattcgaat ttgcccgact gcatcccatc gatcatggct
     1621 ccgaagatga agccgaggat accgccgatc ggtccgcccc aggcccagcc caggccccca
     1681 ccgatccatt tggcgaaacg taattttgcc atgtcttttt tttattgcgg caaagttagg
     1741 aaatagttaa gcagttggac cattaactgt aagcaccaaa ttccaaattc caaattccaa
     1801 acaaattcaa aaaatcaaat tcaaaatagt attggttttt gaacttttaa tttggtgctt
     1861 gtttggaatt tggtgcttgg tatttggtgc ctggaattta gtgcctggaa tttaagagat
     1921 ccgttcactt accgataatc catattcctc caccatcttt atattcatgg tttcgagctg
     1981 atccaggatc gggttgtaaa tgtcggggat cacgggaatg taannnnnnn nnnatgcagc
     2041 gggcaatgca actgtccggg caacggatgt gtcagtcttc aaagttccga aatcgagcat
     2101 gcgggatttg atgacttcct gctttccatc gggatagctt gccaggaaag tatgctgcat
     2161 caccaccatg tcgcgttctt cgggctggat catcatttta gcgatcatca ggtcggaaac
     2221 aacttcgaag gtgctgtctt ccatgcggtt catcggctca ttgctgaaca gtccgagcca
     2281 ctccatggcc acgatagcgc gggcattgat cttcacgcca agttttgcgg ccactttcgc
     2341 cacaatattt tccgtagagc tttccccgat cattttcgcc atcatcccgg catatgacat
     2401 gccttccata ttgaatttat cgtaggttat aagcttgagg actttcaacg cgtcgatgat
     2461 ctcgcaccag ccttcatagc ggaacgtgcc gcgcataatg gtcttcgctt cgggaatgcc
     2521 gtataattca acataaggaa gagaatcgcg gtttgggtaa acatcgagta acccgacttt
     2581 cgggaaatcg actttcagcg ggttcctgaa aagttgctcg gtgggaatat atttcaccac
     2641 gccatctttc aaaaaattcc cgtcattatt tccggccatg accacacctt tgggagccca
     2701 ggtaaattta taatggaaag ggttttcttc cacttccggt gcaacgagag cgccgcagaa
     2761 agaatagaac tcatccacat taccaccttt tccgtgaata tggtcgatga tgcgcatggc
     2821 cgacatatgg tcgatgcccg gatcgagacc cagttcgttc aggatgataa tgccggcagc
     2881 tttggcttcc gcatcaaggg ctttcatttc cggcttcaca taggaagtgg tcaccatgtt
     2941 ctttttcagg cggatgcatt tctttgccac catcacatga tagagataag gcagcagact
     3001 gacggtcagg tcgtgttcgg cgatcatctg atccagttcg gcctcctgtt cgacggtcca
     3061 tgctaccgaa cggccattgg gatgcccgcc gatcatttcg tcggctttcg atttagtgcg
     3121 ggtagctacg gttacgataa agtttttgtc gagtaaataa cgcacgatgg gatttaccac
     3181 catgccggct ccaaggataa ggactttttt catgagttct attgttctat tattcgattg
     3241 ttaaattgtt aaactgttac aggttccaaa tttccaattt ccattttcca atttagatat
     3301 acttctgcaa gtactcataa tccggggtga gttcgccttt gtacaggatg gtggctttac
     3361 ggatcgggcc gggcagtttt actttctcaa aaggctggct gaaatcggtt ttggcaattg
     3421 gggccacata ccgccagagc acctcgctaa aaaaacgtga agcatcgcgg ggcagctcac
     3481 tgggcagaat atctaccgcc atgacgagca atcctttccc tttgaaacca tccgttgctt
     3541 ttcgagtgga cggattgtaa acaaataccg ggttttcgat ctccgttcct ctatgcgtga
     3601 tctcgacgga accatccgga tcgcaggtaa tatccccgat aacggtcagc ctgggctttc
     3661 catctttaaa aagctgctcg gtaaattctt tagttaaaag tcttggatac cgtgggtccc
     3721 agtagataca attcaccagg accgtcaggt aagggatgta ttgctcaaac tggctgcagt
     3781 aattttccgg gtgctggtaa tagtcctgca gatcgaacgc atgatccgga tttttcggtt
     3841 tgaccaggtg ttcttccttg aaaactacct tgtatatgat attatccggg aggtgggtat
     3901 gtttatggag gttgaaaagt tcatccggca gaatctcctt aacgggcagc agcgaagcga
     3961 tctcctgggc gccctgcgac acgttcccgt aacccgtgaa cccgataacc agtggtttga
     4021 gctccggggg aaggcccttt tcaatgatct tctgacctac ttttgatacc accttccgca
     4081 cttcatccag tgaatgataa ttatgggttt gtgcgatatc gagaaatggt gtaataacgc
     4141 cgaattcctc aagtctttgc cctaatgacc agagcgaatt gatcatccct gccaggccgg
     4201 catatcttcc gaaaaagatc agccttttgc ccatttcatc cacaaccctt tcatagtcga
     4261 tcaacgtgca attcagctcc atcaatcgtt tcagcattgg catattgtgc ggctggcctt
     4321 tgatcacatg ggaaaagaaa atatacgttt tg
//
LOCUS       DAOQXS010000244         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20648_length_4344_cov_3.815342, whole genome shotgun sequence.
ACCESSION   DAOQXS010000244 DAOQXS010000000
VERSION     DAOQXS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20648_length_4344_cov_3.815342"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKM34_07515"
     CDS             complement(<1..1115)
                     /locus_tag="PKM34_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83474.1"
                     /translation="MSNHGHHHRSHRRRSALGRFIDRLTGRRRRSSFHRSEFPGIHLP
                     GEKEEGTRKYRILEERSAPDTSPEITGTGANETAGQSASTGSSSRRRRKESFFKLYLK
                     KRALRKEEQYKEKMRRAHRRRSEKEYRKLNQGPGLGKKLLDLARDEEEAAKKIPLLSN
                     RSRFFRNMTIVVNSMMIFMITYVLTYLFYWLSCMLVASWYGLDSILYFYDLRFNDHSP
                     LWSRFNILLITGIPPFLCLVAGAFLYKAVFKIKRFAGLQKLFILWSAFHLLNHFFGAF
                     PSGVVTDEGLGYVAAWMYMNTAVKFLFSLLFLFGLGLIGYYSAKPILETSDSMGRIKS
                     ERKTSFMFYQMGLPWLIGTFVMLLLRIPKNFDYPYETP"
     gene            1191..1790
                     /locus_tag="PKM34_07520"
     CDS             1191..1790
                     /locus_tag="PKM34_07520"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNQ83475.1"
                     /translation="MKKLYEGLNKSIKKYKLYVLKDVIIFIIITLAVHFTYRFWVNIL
                     DYWPVNPLMHRAHDFMSSVVYNQSSVIIGNILNIPFTEGENRFFYFENTGFIAINESC
                     SGLKPMLQFLLLMIIFPGPWKHKAWFIPMGLVIVHLTNLFRISGLAVVTITLPEYWDF
                     AHDNIFRPFFYVVIFLLWVWWAEKFRPRFAKRIKTENSG"
     gene            complement(1795..2721)
                     /locus_tag="PKM34_07525"
     CDS             complement(1795..2721)
                     /locus_tag="PKM34_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83476.1"
                     /translation="METNPNTNISGGRSNRTLITILVISAIVVIGLIIILLQTRSNLK
                     GLLTEKEQQRQELTRELDSLLVEHNKVKTEYGRLSDSLVSKDSVIQANAEEIKRLLNT
                     EWEYYKVRKKLAQLQTVAQGYVLQMDSLYRVNASLTEENTAIKKDLKDLRKEKEQIES
                     DRKVLTGKVEMAAVLKAFNMDAVGLRLRSGGEKEVTTDKAQRIDQVRICFNIAENKIA
                     TPGSKEIYIRIARPDQEILTKSQIDDYSFEYQGERLQYSVMETIEYENEEQEVCVYWK
                     KEYSSQEMQAGLYHVDIFCENAVIGHTTFTLR"
     gene            complement(2762..3928)
                     /locus_tag="PKM34_07530"
     CDS             complement(2762..3928)
                     /locus_tag="PKM34_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNQ83477.1"
                     /translation="MHYHFDTIIERAGTSSVKYDLRKALFGRDDVIPMWVADMDFAVP
                     HFVEEAVLNRARHPIYGYTAIPESYTEAVAGWQQSRHNWEIDNNWLFFSPGVVAGLNM
                     IVQAFTRPGDAVIVQPPVYFPFFSCVENNGRRLVLNQLKEQNGDYTIDFEDLEQKMKA
                     GARMMILCSPHNPVSRSWSREELERLGEKCLEYGILMVSDEIHGDLVFGPHRHIPMAS
                     ISPEIADNTITCIAPSKTFNLAGLFTSSIIIPNEKLRETFKKHQETLHLSPNLFGLVA
                     AEAAYREGGSWLDELMQYLKDNISIVSGYLSRNLPDITFSKAEATYMLWLDFRKTGIP
                     PAEMKSRLVEKAGLGLVEGPVFGQGGNGFQRMNIACPRTVLIGALERLPLALNP"
     gene            complement(4109..>4344)
                     /locus_tag="PKM34_07535"
     CDS             complement(4109..>4344)
                     /locus_tag="PKM34_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNQ83478.1"
                     /translation="LTDFSVNAGNNTVADWKKFYGYLFTRFMDGNIKTAVPVPDGYIY
                     HAPKVEQPGYPEWYLRLIVETTGDKLKVIGGGH"
BASE COUNT         1043 a         1098 c         1023 g         1180 t
ORIGIN      
        1 ggggtttcat acgggtaatc gaagtttttg gggattctga gcaaaagcat tacgaaggtg
       61 ccgatcagcc agggcaatcc catctggtag aacatgaacg atgtttttct ctccgatttg
      121 atccggccca tactgtcgga tgtttccagg atcggtttgg cggaataata accgatcagc
      181 cccaatccga ataaaaacag cagggagaag aggaacttca cagcggtgtt catgtacatc
      241 caggcggcca catagcccag cccctcgtcg gttacgacac ccgacgggaa agcgccgaag
      301 aaatggttca ggagatggaa agccgaccag aggataaaaa gtttctgtaa tccggcaaat
      361 cttttgatct taaaaacggc tttgtaaaga aaagcgcctg ccaccaggca cagaaacggg
      421 ggaatacccg ttatgagcag gatattgaac cggctccaca acggcgagtg atcattgaat
      481 ctcagatcgt agaaatagag gatggaatcc agcccgtacc aggaagccac cagcatacag
      541 ctcagccagt agaacaggta ggtcagtacg taggtgatca tgaagatcat catcgaattc
      601 accacgatgg tcatgttcct gaaaaagcgg ctccggttcg acagcagggg gatctttttt
      661 gctgcctctt cttcgtccct ggccaggtca agcagttttt tgcctaatcc ggggccctgg
      721 ttcagtttcc ggtattcttt ctctgatctc cgccggtggg cccttctcat tttctctttg
      781 tactgctctt cttttctcag cgcccttttt ttcaggtaga gcttaaagaa gctttcctta
      841 cgccttcggc gggaagacga tccggttgac gctgactgcc cggctgtttc attggcgccg
      901 gttccggtaa tttcaggtga tgtatccgga gcacttctct cttccaggat ccggtatttc
      961 ctggttccct cttctttttc gccgggaaga tggattcccg ggaattccga acggtgaaat
     1021 gaactgcgcc gccgccggcc tgtcagcctg tcaataaacc tgcccagggc gctgcgcctg
     1081 cggtgacttc tgtgatggtg gccgtgattg gacattttct ccggattaat aaacaaaggc
     1141 tgtaaaaaat cttaccttag ccgcatactt cctcatgata ctggcaaagg atgaaaaagt
     1201 tatacgaagg gcttaacaaa agtataaaaa aatataagct atatgtattg aaggatgtga
     1261 taatctttat catcatcacc cttgccgttc actttactta ccgcttctgg gtcaatattc
     1321 tcgattactg gccggtcaat ccgctgatgc accgggctca tgatttcatg tcaagtgtgg
     1381 tttataacca gagctcggtg attatcggca atatcctgaa tatccctttc acagaagggg
     1441 agaaccggtt cttctacttt gagaacaccg gtttcattgc cataaacgaa agctgttcgg
     1501 gactgaaacc gatgctgcag tttttgctgc tgatgattat ctttcccggg ccctggaagc
     1561 ataaagcctg gtttatcccg atggggcttg tcattgtcca cctcaccaac ctgttcagga
     1621 taagcggcct cgccgttgtg accattactt taccagagta ctgggatttt gcccacgaca
     1681 atattttcag gccattcttt tatgtagtga ttttcctgct ctgggtctgg tgggcggaga
     1741 aattcagacc cagattcgcg aagcggatta aaaccgaaaa ttctggttaa tcctttatct
     1801 cagagtaaat gttgtatgac cgataacggc attttcacag aaaatgtcga catggtaaag
     1861 acccgcctgc atctcctggc ttgagtattc tttcttccag taaacgcaga cctcctgctc
     1921 ctcattttcg tattcaatgg tctccatgac agagtactgc aatctttcgc cctggtactc
     1981 aaaagaataa tcgtcaattt gacttttagt caggatctcc tggtcgggac gggcaatgcg
     2041 gatatagatc tcttttgagc cgggagtggc gattttattt tccgcaatgt tgaaacagat
     2101 ccttacctgg tctattcgct gggctttgtc ggtggtcacc tctttctccc cgccgctgcg
     2161 cagtctcagg ccgacggcgt ccatattgaa agctttcagg acggcggcca tttccacctt
     2221 gcccgtcagc actttgcggt cgctttcaat ctgctccttt tccttgcgta aatctttcaa
     2281 atctttcttg atcgctgtgt tttcctctgt caggctggcg ttgacccggt aaagcgaatc
     2341 catctgcagg acatatccct gtgccacagt ctgcagttgg gccagctttt tcctgacttt
     2401 gtagtattcc cattccgtgt tcagcaaccg ttttatctcc tcagcattgg cctggatcac
     2461 gctgtctttg gaaacaagcg aatcggaaag gcgcccgtat tccgttttga ccttattgtg
     2521 ttcgaccagg agtgaatcca gttcccgcgt aagttcctgc cgctgctgct ctttttctgt
     2581 cagcaggcct ttcaggttgc ttctggtttg taataaaatg attatcagcc caatgaccac
     2641 gatcgcagaa atcacgagga tcgttattaa agtacgattt gaccggccac cggaaatgtt
     2701 tgtatttgga ttggtttcca taaatcaatt atttaacaca aaaataagaa taaaattgcc
     2761 gtcagggatt gagtgccagg ggtaatcttt ctaatgcccc gatcagaaca gtgcgcggac
     2821 aggcaatatt catacgctga aatccattcc cgccctgtcc gaataccggt ccttctacaa
     2881 gtcccagccc cgccttctcc accaggcggg atttcatttc agccggcggt atgccggttt
     2941 tgcggaaatc gagccagagc atataggtgg cttctgcctt actgaaggtg atgtcaggga
     3001 gattccggga aaggtaaccg gaaacgatgc tgatattatc tttcagatat tgcatcagct
     3061 catccagcca cgaaccccct tcccggtaag ctgcttccgc cgcaacaagc ccgaacaggt
     3121 tcgggctgag gtgaagggtc tcctggtgtt ttttaaatgt ttcccttagt ttttcattcg
     3181 ggatgatgat ggaagaggtg aataatccgg ccaggttgaa ggtcttgctc ggagcaatgc
     3241 aggtgatggt gttatccgct atctcaggcg agatggaagc catgggaata tgacggtgag
     3301 gcccgaatac caggtcgcca tgaatttcgt cactaaccat cagaatcccg tattcaaggc
     3361 atttttcacc cagccgttcc agttcttccc tgctccagct ccggcttacc gggttatgcg
     3421 ggctgcacag gatcatcatc ctggccccgg ccttcatctt ctgttccaga tcttcaaagt
     3481 cgatggtata gtcgccgttt tgttctttaa gctgatttaa aacgagcctc ctgccattgt
     3541 tttcaacaca cgaaaagaag ggaaaataaa ccgggggctg aacgatcacg gcatcaccgg
     3601 gccgggtaaa ggcctgcacg atcatattca ggccggcgac aacaccgggg ctgaaaaaca
     3661 gccagttgtt atcgatttcc cagttatggc ggctctgttg ccagccggcc accgcctccg
     3721 tgtagctttc aggaatggcc gtataaccat aaatggggtg cctggcgcgg tttaaaaccg
     3781 cttcctcaac aaagtgcgga acggcaaaat ccatgtcagc cacccacatc ggtatcacat
     3841 cgtctctgcc gaaaagggct tttcgcaagt cgtatttcac ggaacttgtt ccagcacgtt
     3901 cgatgattgt atcaaaatgg taatgcattt taatgtgatg tcaaaaagac tttaaatgaa
     3961 aaaatccgtg taaattcaat tgctccgaat agaacacgaa tttacacgga ttcgacggat
     4021 caacaccgat taataattta taaaatcata ccgtccaatc cccaaatccc caaatctcca
     4081 aatctccaaa tctccaaatc gtccaatcct aatgtccgcc tccgatcacc ttcagcttgt
     4141 cgccggtggt ttcaacgata agcctcaggt accattccgg gtaaccgggc tgctcgacct
     4201 ttggcgcatg gtaaatatag ccgtcgggca cagggactgc ggtcttgata ttgccgtcca
     4261 tgaagcgggt gaacaggtag ccatagaact ttttccagtc tgcaaccgta ttgttgccgg
     4321 cattcacgct gaaatcggtc agaa
//
LOCUS       DAOQXS010000245         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20673_length_4341_cov_2.693887, whole genome shotgun sequence.
ACCESSION   DAOQXS010000245 DAOQXS010000000
VERSION     DAOQXS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20673_length_4341_cov_2.693887"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKM34_07540"
     CDS             <1..237
                     /locus_tag="PKM34_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ83479.1"
                     /translation="LSLEFSKWIVVASVIAWPVAWLLVDKWLQSFAYRIEILHFAWIF
                     IASAVIALFIAQTTILYHAYRAATRNPVDAVKWE"
     gene            325..3030
                     /locus_tag="PKM34_07545"
     CDS             325..3030
                     /locus_tag="PKM34_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNQ83480.1"
                     /translation="MKMLIFSLLCLPVFIQAQDLSWNITFAGTNASSTVDSVMVENLT
                     QQTSISLGGNDLLILDVTLAISEPGHLISNALHIFPNPANGACTFQLPSSFSSKSSLE
                     VFDISGKLIFRSEEILEPGMNYFTLDGLTGGVFVIKIKTAQTVYSGKIISMNSTGNPH
                     GLSLTRSGSSASSGGSGVHPESMKTTAAEFIMPYTAGDLLKFRGKAGLHKAVVMLVPT
                     GDREITFNFIPCVDADGRHYATVRIGSQFWMAENLDVGVMINSSQQQTDNGIIEKYCY
                     DNDETNGETYGSLYQWGELMQYGNEGAKGLCPDGFHIPTRDEYNSMIYYLGGSTQAGG
                     KLKERGYEHWNYPNTSATDKSGFEALPNGASKSDGTFDGLKSFAKIWASSPAGTLNAW
                     LAFLSDQSGAIGLYAYEGPEGYSCRCIQDNAEEMTMLAGDTAKTWKLIRDVTTARYPM
                     EVGPYDHSTIWWAMGLNNDELGNRHCMLNDEWTFGRDGSLVFNAYGDYWAEGGIFDPA
                     NFCESTDYMININGEDCSGWGNGNHQFALVTGDNPRLTTLGHGAYLGFYKTATDYEVI
                     QLDPMVQDSVRYNLVMLSDGETDTLIVEVPYYFYPGDLQYGGYWRYVLVHYDNPADEP
                     PLPGPVPTADFTAVSDGLTVTCTNLSLYGEDYLWDFGDGTTDTAFNAVHTYAYDSVFH
                     ITLTVTNPNGSDMTSQDVWISEYPLTEDMLQGGPWSVRVQENSIFVGSGLGLNDWWAV
                     PLANLDGSMIGTTEDWSCITDDEFTFGAGGSYTYQAFGDVRNDGYFGSPNGCWAESAL
                     YGNALYFASGSHTYAFTPANGTERPIITLTNGANRAAFIGFYKGFYGGENYSIENPPN
                     GGFPTNTYEVMGYCIGEGTDYLFISVDISSDHSGTAAWSVILQRD"
     gene            complement(3046..4137)
                     /locus_tag="PKM34_07550"
     CDS             complement(3046..4137)
                     /locus_tag="PKM34_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNQ83481.1"
                     /translation="MLFKNILITGTGSYIPSAIVKNQDFADNKFYDNDGSAFDSSHDE
                     ISEKFLAITGIRERRYISDELVASDIGAKAAEIAIKDSGINREEIDQIIVAHNFGDVR
                     KHTIQTDTVPSLAARIKQILKIENPFCVSYDILFGCPGWIQGLIQAESFIRSGMAKKC
                     LVIGTETLSRVVDMHDRDSMIYADGAGACILELVESDRQEGILSHIAASYTTKEAFYI
                     YLGKGNIEEADPNIRYIKMYGRKVYEFALTHVPAAMKTCLDRAGADLRDVKKIFIHQA
                     NEKMDDEIIKRLYRMYKIHDVPKDITPMSIHTLGNSSVATVPTLYDCVRKGTGEEMQH
                     RLHEGDLILFASVGAGMNINAIAYRYSGI"
BASE COUNT         1049 a         1059 c         1112 g         1121 t
ORIGIN      
        1 ctttcgctgg aattcagcaa atggatcgtg gtcgcgtcgg taatcgcctg gcctgttgcc
       61 tggctactgg tcgacaaatg gctgcaatca tttgcctacc ggattgagat attgcatttt
      121 gcctggattt tcattgcatc ggccgtcatc gcgctgttca tagcccagac cacaatactc
      181 tatcatgctt accgcgccgc aacacgcaat ccggttgacg cggtgaagtg ggaataggga
      241 atggtgaata gtgaatagtg aatagtgaat ggttggggga tattgttgta tctttagggg
      301 taaataatta ccaaaccttt cacaatgaaa atgctcatct tttcgctgtt gtgccttcct
      361 gtttttattc aggcacagga cctcagctgg aacatcactt ttgccgggac caatgccagc
      421 agcacagtcg actcggtgat ggtggagaat ctaacacagc aaaccagtat ctccctcggg
      481 ggaaatgacc tgctgatcct cgatgtgacc ctggcgatca gtgaacccgg ccatttaatt
      541 tccaatgccc ttcacatttt ccctaatccc gcaaacgggg cgtgcacctt tcaattgccc
      601 tcttcgtttt catcaaaatc ttcgctcgag gtttttgata tttcaggcaa attgattttc
      661 cggtcggaag aaatccttga gcccggaatg aattatttca ctttagacgg ccttaccggc
      721 ggtgtgtttg ttataaagat caaaactgca caaaccgttt attcgggtaa gatcatcagc
      781 atgaattcca caggcaaccc tcacggattg agcctgaccc gttcaggctc ttccgcatca
      841 tcgggcggtt cgggagtcca tccggaaagc atgaaaacga ccgctgcgga gttcatcatg
      901 ccttacaccg ccggcgacct gctgaaattc cgcggcaaag cgggattgca caaggctgtc
      961 gtgatgctgg tgccgaccgg tgaccgggaa attactttca atttcatacc ctgcgtcgac
     1021 gccgacggac ggcattatgc aacggtccgg atcggaagcc agttctggat ggccgaaaac
     1081 ctggatgtcg gggtgatgat caacagcagc cagcagcaaa ccgacaacgg gatcattgaa
     1141 aagtactgtt atgataatga tgagaccaat ggcgaaacgt acggttccct ttaccaatgg
     1201 ggtgagctga tgcaatacgg taatgaaggg gctaaagggc tctgtcctga cggatttcat
     1261 attcctaccc gcgatgaata taacagcatg atatactacc tcggcggctc gacgcaagcg
     1321 ggcggtaagc tgaaagagag gggatatgag cactggaatt accccaatac ttcggcgaca
     1381 gataaaagcg ggtttgaagc actgccaaat ggcgccagca agagtgacgg cactttcgac
     1441 gggctaaaat cttttgctaa aatatgggca tcatcaccgg cagggacttt gaatgcctgg
     1501 ctcgctttcc tgtcggatca gagcggcgct atcggcctgt atgcttatga aggcccggag
     1561 gggtacagtt gccgctgcat ccaggacaat gccgaagaga tgaccatgct ggccggcgat
     1621 acggctaaaa cctggaaact catccgtgat gtgactacgg cccgttaccc gatggaagtc
     1681 ggtccgtacg accattctac gatatggtgg gccatggggc tgaataatga cgaactgggc
     1741 aaccgccatt gcatgctcaa cgacgaatgg acctttggcc gcgacggatc gctggtattc
     1801 aatgcatacg gagattactg ggccgaaggg ggaatttttg atccggctaa cttttgcgag
     1861 agtacagatt atatgatcaa cataaatggc gaggattgct ccggctgggg caacggcaat
     1921 catcaattcg ccctggtaac aggggataat cccaggctca ccaccctggg tcacggggca
     1981 tatctcggct tttacaagac cgcgaccgat tatgaagtga tccagttgga tcccatggtg
     2041 caggattctg tcaggtataa cctggtgatg ctgtcagatg gggaaaccga cacgttgatt
     2101 gtggaggtac cctattattt ttacccgggc gacttgcaat acggtggcta ctggcgctat
     2161 gtcctggtcc attatgacaa tccggccgac gaaccgcccc ttcctggtcc agtgccaaca
     2221 gccgatttta ccgctgtttc cgacggactt acagtaacct gcaccaatct gtcgttgtac
     2281 ggagaagatt acctctggga ctttggcgat ggaaccaccg atactgcgtt taatgccgtt
     2341 catacttatg cctacgatag tgtgttccat attacgctga ccgtgaccaa tccgaacgga
     2401 agcgatatga cttcccagga tgtctggatc tctgaatacc ctttgactga agatatgctt
     2461 cagggcgggc cgtggagcgt cagggtacag gaaaactcca tattcgtggg ttcagggctg
     2521 ggattgaacg attggtgggc ggttccgctg gccaacctgg atggatcgat gattggcacg
     2581 actgaagact ggtcgtgtat taccgatgat gaatttacat ttggcgcagg gggatcatac
     2641 acctaccagg cttttggcga cgtcaggaat gacgggtatt tcggcagtcc gaacgggtgc
     2701 tgggccgaat cggcactgta tggcaatgcc ctctattttg cctccggaag tcatacgtat
     2761 gcctttaccc cggcaaacgg cactgaacgt cccatcatta cgctgaccaa tggggcgaac
     2821 cgggcagcat ttatcgggtt ttacaaaggt ttttacggag gtgagaatta tagtattgaa
     2881 aatcccccga acggaggttt tccgaccaat acttatgaag tgatgggata ttgcattggg
     2941 gaagggaccg attacctgtt catatccgtg gatatctcat cggatcattc cggtacagct
     3001 gcctggtctg ttattctgca acgggattag aaaccggcac cggcttcaga taccggaata
     3061 gcggtaggcg atcgcattga tattcattcc cgcgccaacg gaagcgaaca ggatcagatc
     3121 gccttcatgg agcctgtgct gcatctcttc tcccgtccct tttctgacac agtcgtacaa
     3181 agtagggacc gtggccacgg agctgttgcc cagcgtatgg atgctcatcg gggtaatatc
     3241 tttcgggaca tcatggatct tatacatccg gtaaagccgt ttgataatct catcatccat
     3301 tttttcgttg gcctggtgga tgaatatctt cttaacgtcc ctcagatcag ctcctgcacg
     3361 atctaggcag gttttcatag ccgcaggaac atgggtcagg gcaaattcgt atactttcct
     3421 tccgtacatt ttaatgtagc ggatatttgg atcggcttcc tctatattgc ctttcccaag
     3481 gtaaatatag aaggcttcct ttgtggtgta ggaggcggca atatgggaaa ggatcccttc
     3541 ctgccggtcg ctttcgacca gttccaggat gcaggcgccg gcgccatcgg catagatcat
     3601 ggaatcgcgg tcgtgcatgt caaccacccg cgaaagggtt tcggttccaa tcaccaggca
     3661 cttttttgcc atccccgatc gaataaagga ttccgcctgg atcaggccct ggatccatcc
     3721 cgggcatcca aataaaatat cataactgac acaaaaagga ttctcaatct tcaggatttg
     3781 cttgatccgg gctgccaggc tcggcacggt atcggtctgg atggtatgtt tcctgacatc
     3841 cccgaaatta tgggccacga tgatctggtc gatctcctcc cggttgatcc cggaatcttt
     3901 aatggcaatt tcagccgctt ttgcgcctat atccgaagcc acaagttcgt cggaaatata
     3961 ccgccgttcc cttatccccg tgatggccag aaacttctcc gaaatctcat cgtgtgagct
     4021 gtcgaaagcc gatccatcgt tatcgtaaaa tttattatcg gcaaaatcct gatttttaac
     4081 aattgcagag gggatataac tgccagttcc cgttatcagg atatttttaa aaagcatact
     4141 tactggttct ataactaagc tacaaaatta gtaagaattt accttaagat attacatatc
     4201 attaaagtta gttcgattag ttttataaaa atcgggtatg ggatggtctc aaaagtccaa
     4261 tattcgcttt ttataggtag tccgttgagc gtcccgtcgg ttccggatgg gttttccttc
     4321 cgacgactaa agccgtcggt t
//
LOCUS       DAOQXS010000246         4334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20704_length_4334_cov_3.688712, whole genome shotgun sequence.
ACCESSION   DAOQXS010000246 DAOQXS010000000
VERSION     DAOQXS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20704_length_4334_cov_3.688712"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKM34_07555"
     CDS             complement(<1..1079)
                     /locus_tag="PKM34_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ83482.1"
                     /translation="MMREAILGISAYYHDSAAAIIVDGEIVAAAQEERFTRKKHDSSF
                     PANAVNFVLDEAGIDPGTLRAVVFYEKPLIKFERLLETYHAFAPRGLNSFLVAIPAWV
                     KEKLFMKKMLRAELEKIHKAKYRLLFPEHHLSHAASAFFPSPFNEAAILTIDGVGEWA
                     TTTIGYGNGNRIEIMKELHFPHSIGLLYSAFTYYTGFRVNSGEYKLMGLAPYGINGSG
                     QVGDFIEKIKSELVDIRDDGSILLNMSYFRYATGLKMTDDKKWERLFGFPPRKESDPV
                     TLDYMNMALAIQQVTEEIVLKLARTAKELTGSRNLAMAGGVALNSVANGKLLAEKIFD
                     DIWIQPAAGDAGGAVGAALAAWHIW"
     gene            complement(1133..1282)
                     /locus_tag="PKM34_07560"
     CDS             complement(1133..1282)
                     /locus_tag="PKM34_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNQ83483.1"
                     /translation="MEYIKDLWDYLKVRKKYWLVPLILILLLLGFLIVFTGGSAVAPF
                     IYTLF"
     gene            complement(1305..1709)
                     /locus_tag="PKM34_07565"
     CDS             complement(1305..1709)
                     /locus_tag="PKM34_07565"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNQ83484.1"
                     /translation="MKNDEERIFPGKISRERIKDSGMALVLILLIIGLITDSSTYFIL
                     SAAFLLVNMIFPPFFYPFAFIWLGFSEILGRVMSKIILLLVYLAVVLPVAFIRRLLGK
                     DTLLLRKFKDGKGSVMVDRRHDFQAEDLEKPF"
     gene            complement(1770..2186)
                     /locus_tag="PKM34_07570"
     CDS             complement(1770..2186)
                     /locus_tag="PKM34_07570"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNQ83485.1"
                     /translation="MTYPFINIWAVLISAVAFWALGALWYSPVLFGKRWQKEIGATDD
                     ELKKANMALIFGGSFLLMLFMVWALNFVINSHKAEDVSLTMGLNYGLFTGFFFSMMTM
                     GINYLYQRRSVVLWLIDGIYVVLGLGIAGMILGAWR"
     gene            2509..3201
                     /gene="aqpZ"
                     /locus_tag="PKM34_07575"
     CDS             2509..3201
                     /gene="aqpZ"
                     /locus_tag="PKM34_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNQ83486.1"
                     /translation="MKKYLAEFIGTFSLVLFGCGAAVIAGTSATGPSGVGLLGISIAF
                     GFAVVAMAYAIGGVSGCHINPAVTIGVLTAGKMSVKDAAGYIISQCLGGIAGAAVLYL
                     IVMGRPGFAMPEWGLGANGWGTGYLAEYNTLSAFVIEAVMTCLFIFVILATTSKFGNG
                     AMAGLAIGVTLMLIHLVAIPVTGTSVNPARSLGPAVFAGGQALAQLWLFFVAPIIGAV
                     AGALIWKFGFEK"
     gene            complement(3250..>4334)
                     /locus_tag="PKM34_07580"
     CDS             complement(3250..>4334)
                     /locus_tag="PKM34_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83487.1"
                     /translation="TVCHSPAMPRIKGFGEEFAGNGFRLKDTDAPRYFVSTGDDKLSL
                     LRELPLAIRLEGHVMYNDQSEAGKEFGFPYGLKILSGGEISKHLSYYFYFYMSEQGEV
                     AGVEDAFLAYNDFLGTGINITAGQFQVCDPLYKRELRLMLEDYVIYTLKPGTSTVSLK
                     YDRGIIIDYGIESTGTGFVAEIVNGNGIHGAGKNFLFDKDKYKNYFLKGSQSIGKFAD
                     IGIFGYYGKEELEPDGLTSTVLFWGPNLTLDFGEKFILNMQYARRSDSKVVIEGEVTE
                     IKSDVFTHGGFAEAIFAPKGDMSNWYLALLGNWVDSELDALDYSSVSFSAGYMLRRNV
                     RLVGEYTRQFANEPYGKASIGFVAAF"
BASE COUNT         1055 a         1095 c         1081 g         1103 t
ORIGIN      
        1 ttccatatat gccaggctgc cagggcggca cccacagcgc caccggcatc tcccgcggcg
       61 ggctggatcc agatatcatc gaagatcttt tcagcgagca gctttccgtt ggccacactg
      121 ttcagcgcca cgccgccggc catggccagg ttgcggctgc ctgtgagttc ttttgccgta
      181 cgggccagtt ttagcacaat ttcttcggtc acctgctgaa tggccaacgc catgttcata
      241 taatccaagg tcaccggatc ggattctttt cgtggcggga atccgaacaa ccgttcccat
      301 tttttatcat ccgtcatctt caaccctgta gcataacgga aataactcat attcagaagg
      361 atcgaaccgt cgtcgcggat atctaccagc tcacttttta tcttttcgat gaaatcgccg
      421 acctgcccgg agccatttat gccatagggt gccaggccca tcagcttgta ctcgccgctg
      481 tttaccctga accccgtata gtaagtaaag gctgagtata gcaatccgat ggagtgggga
      541 aagtggagtt ctttcatgat ttcaatccgg tttccattcc catagccgat tgtggtggtg
      601 gcccattccc cgaccccgtc gatcgtcagg atcgccgctt cattaaaagg ggaggggaag
      661 aaagcgctgg cggcatgcga aaggtggtgc tccgggaata aaagccggta tttcgctttg
      721 tgtattttct ccagttcggc cctgagcatc tttttcatga aaagtttttc ctttacccag
      781 gctggaatgg ccaccaagaa actattcaat ccccggggcg caaaagcgtg gtaggtttcc
      841 aggagccgct cgaatttgat cagcggtttt tcatagaaaa caacggcccg gagtgttccg
      901 ggatcgattc ccgcttcatc caggacgaaa ttaacggcat tggccggaaa agaagaatcg
      961 tgcttcttcc gggtaaaccg ctcctcctgg gcggcagcca cgatctcgcc gtcgacgatg
     1021 atggctgcgg cgctgtcgtg gtaatacgcc gatattccca ggattgcttc ccgcatcatc
     1081 agaacataat atttctcaaa aaaaataaca tacgctttgg ccttaccatt ctttaaaaaa
     1141 gagtatagat aaacggtgca acggccgatc caccggtaaa gactatcagg aagcccagca
     1201 acagcaggat gaggatcagc gggacgagcc agtatttctt tcgcactttc agataatccc
     1261 agaggtcttt aatatattcc ataagacact gtttataaag attttcaaaa tggtttttcg
     1321 agatcttccg cctggaaatc atgcctccgg tcaaccatga ccgatccttt cccgtctttg
     1381 aatttcctga gaagcagggt gtcttttccc aataatctcc tgataaaggc aacgggtaat
     1441 acaacggcaa gataaaccag cagaaggatt attttcgaca tgacccgtcc caggatttcc
     1501 gaaaatccga gccagataaa ggcaaacgga tagaaaaacg gggggaatat catgtttacc
     1561 aggaggaatg cggcggagag aataaaatac gtggagctat cggtaattaa tccgatgatg
     1621 agcaggatca gcacgagggc cattccgctg tctttaatcc gttccctgct tatttttcca
     1681 gggaatatcc tttcctcatc atttttcatg ctttaaaaat acacagaaaa atgatcggaa
     1741 agaaaggata acgttctaag gatgtcagat catctccacg cgcccaggat catcccggca
     1801 attccgagtc ccaggacgac gtagatcccg tcgatcagcc agaggactac agagcggcgc
     1861 tggtagagat aattgatccc catggtcatc atggaaaaga aaaacccggt aaagaggcca
     1921 tagttcagac ccatggtgag gctgacatct tccgctttat gggagttgat cacgaaattg
     1981 agcgcccaga ccatgaacaa catcaacagg aaactccccc cgaaaatgag ggccatattg
     2041 gcttttttaa gttcatcatc ggtggcgccg atctctttct gccagcgttt gccgaagagg
     2101 acagggctgt accacagggc gcccagggcc cagaaggcga ctgccgaaat aagcaccgcc
     2161 cagatgttga taaagggata ggtcataatg ttgaatgttg aatgttgaat gttgaatgtt
     2221 gaatgttgaa ggttgaatgt tgaaggttga atgttgaatg ttgaatgttg aaggttgagt
     2281 ttagttgctg gttgttagtc aatggtttgt tatgggttac ggttggtttc acgcttcacg
     2341 cttcactttc ttacagcaaa aatagtaatt ggaacagaac ctcttgttaa tgagatgtta
     2401 aaatcgatga gcgtatggaa tatctttcta aataatatta ttggtttcaa ggtacttatt
     2461 agttaacttt atgaccaatt catcaagtca taatctaata tttttattat gaaaaagtat
     2521 cttgcagaat ttatcggaac cttcagcctg gttctgttcg gctgcggtgc ggcagtgatt
     2581 gccggaactt cagcgaccgg cccgagtggc gttggattac tcggcatcag tattgccttc
     2641 ggttttgccg ttgtagccat ggcttatgcc attggtggcg tttcaggctg tcatatcaac
     2701 ccggccgtaa ctattggagt tttaacggca ggaaaaatga gtgtaaagga tgcggcagga
     2761 tacataatct cgcagtgcct gggcggtatc gctggcgcag cggtattata cctgatcgtt
     2821 atgggcaggc cgggatttgc catgccggaa tggggccttg gcgccaatgg atggggcacg
     2881 ggatacctgg cggaatacaa taccctcagc gcttttgtga tcgaagcggt catgacctgc
     2941 ctgttcattt tcgtaatcct ggcgaccacc tcgaaattcg gtaatggagc catggccggg
     3001 ctggccatcg gggttaccct gatgctgatc cacctggtgg cgatccccgt gaccggaacc
     3061 tcggtcaacc cggcgcgtag cctcggaccg gcggtattcg ccggaggaca ggcgctggcc
     3121 cagttgtggc tgttctttgt cgcacccatc atcggcgctg ttgccggcgc actgatctgg
     3181 aaattcggct tcgaaaagta ggaggtaacg ttttcctgta acaaacctca tattaatatc
     3241 aggaaagcct cagaacgcgg ccacaaaccc gatgctggcc ttcccgtagg gttcatttgc
     3301 aaactgccgg gtgtattccc cgaccagcct gacattgcgc cggagcatat acccggcgct
     3361 gaacgaaacg ctgctgtaat ccagcgcgtc gagttccgaa tcgacccagt tgccgagcag
     3421 ggccaggtac cagttgctca tgtcgccttt cggggcaaag atcgcttcgg caaacccgcc
     3481 gtgggtgaag acatcgcttt ttatctccgt cacttcgcct tctatgacga cctttgaatc
     3541 cgaccgacgg gcatattgca tgttcaggat gaatttctca ccgaaatcaa gcgtcaggtt
     3601 cggaccccag aacagcaccg tgctggtcag cccgtcgggt tccagttctt ctttcccata
     3661 gtagccgaaa atccctatgt cggcaaattt cccgatcgac tgcgagcctt tcagaaaata
     3721 gttcttgtat ttgtccttat cgaataggaa attctttccc gccccgtgta tcccgtttcc
     3781 attcacaatc tcggccacaa acccggtacc ggtcgattcg atgccgtaat cgatgatgat
     3841 cccccggtcg tacttcagcg atactgttga tgtgcctggt ttcagcgtgt agatcacata
     3901 atcttccagc atcagccgca attccctttt atacagcgga tcacatacct ggaactggcc
     3961 ggcggtgatg ttgatgcctg ttcccaggaa atcgttatat gccaggaatg cgtcttccac
     4021 accggctact tcaccctgtt ccgacatgta gaaatagaaa taataggaga gatgtttgga
     4081 gatctcgcct cccgaaagga tcttaagtcc gtagggaaat ccgaattctt ttcccgcttc
     4141 gctctgatcg ttgtacatca catggccctc caggcggatg gccagcggca attctctcag
     4201 gagcgaaagc ttgtcgtcgc cggtactcac aaaataacgc ggagcatcgg tatctttcaa
     4261 ccggaacccg ttgccggcaa actcttcacc aaagcctttg atccggggca tggccggcga
     4321 atggcacacc gtgc
//
LOCUS       DAOQXS010000247         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20815_length_4314_cov_4.224231, whole genome shotgun sequence.
ACCESSION   DAOQXS010000247 DAOQXS010000000
VERSION     DAOQXS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20815_length_4314_cov_4.224231"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1044
                     /locus_tag="PKM34_07585"
     CDS             124..1044
                     /locus_tag="PKM34_07585"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNQ83488.1"
                     /translation="MKKLLTILLSATVLFAFAEERIANNPKPINHGPLACPSLVMTPV
                     DNTVTYPSDLIEAILGPDVVSYSLINFAGLAGNPYASAGLFVGGTDAGLGIEDGIVMC
                     SGYITNAIGPNSDDGASVSLGLPGDAQLSAFAGQSTFDATVLEFSFVPNFDQLYIQYV
                     FGSEEYNEYVFQYNDAFAFFLNGNNIAVVPSTTLPVTINTINLSTNNTYYKNNDYGDL
                     YPGPYPYCNEMDGFTTVLVAQGPVNEGESNTIKLAIADGGDSALDSFCFIKGSSFGGT
                     DPEVPVSNWALFIGIGLILIFAVIRFRRLV"
     gene            complement(1126..3408)
                     /locus_tag="PKM34_07590"
     CDS             complement(1126..3408)
                     /locus_tag="PKM34_07590"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNQ83489.1"
                     /translation="MMKKFPAVLCIFFPTLWFTNTLTAQPEFPPNAPVFDSSTVARID
                     ITIDPDTLAWIYENVDSDIESHATFRFENQTIDELVENVGFRLRGNTSRYSQKKSFKV
                     SFNTFEPGKKFQGMEKLNLNGEHNDPSIIRSRICWEWLRTAGVPAPRAAHATVYINGD
                     YYGLYIMVEHVDEEFAESRFGNKNGNLYKCLWPADLDYLGNDPDLYKFYQNGRQAYEL
                     KINEEYNNYSDLAHFIDVLNNTPPEDLECELEKVFNLNDYLKIMALDVITGNWDGYIY
                     NKNNFYLYHNTETGRFEYIPYDLDNTLGIDWMDRDWATRDIYDWEQHGTEVRPLYARL
                     LEVPGIKDRYSFFFKKFLDLLSEEDSLITAIGGIRTMIAPFVMNDPFYPLDYGYTYDN
                     FINSYDQALGGHVDYGLIPYIQTRRTTALQQLVINGIPPVINYIESSKAVPGEEYWIR
                     ATVTDESADFTVKLKYSINGGTELLVNMKDDGTHHDHDEDDGCYGYEFDGFDIGQILS
                     WQVMVTDPESMITVRPCSPVIIDIHTSDDPQIFINELMADNDTTITDEFGEYDNWVEI
                     YNADDHPVWMGDKYLSDNLSNPDKWKLPDVSIPPGGFLLIWTDGQPGQGPFHTDYKLN
                     DEGESLAIFDNESTGYFLIDSISWGLIPIDVSYGREEDGFLSWIYFNHATPGYSNGSF
                     GTDEPEPVVKSTRFYPNPVKNGTIYFDDIFNGCILNATGMIVMRIEGEKITNVGHLRS
                     GLYFLLDSETGRSFKVFIIH"
     gene            complement(3451..>4314)
                     /locus_tag="PKM34_07595"
     CDS             complement(3451..>4314)
                     /locus_tag="PKM34_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNQ83490.1"
                     /translation="DSALHFVKTQTGFGPRVPGSKAHADCASWLRSKLMDYTTDVRVQ
                     EFKTRVYSGETFTGKNIIASFNPEKKIRIMLCAHWDSRPYADYDPDLAKHNLPVMGAN
                     DGASGVGVLLEMARIFKNHPPDIGVDILFFDLEDYGPPKDSQNAANEHWGLGSQFWSH
                     NPHVPGYNARFVILLDMVGAGNARFLKEGFSMHFAPDKVKKVWDIAASLGYQEYFRDE
                     KGAFINDDHYFINEIRKIPAIDIIHLDPNSTNGSFYDYWHTTQDTFDKIDKRTLQVVG
                     DVVTKVVYTEK"
BASE COUNT         1152 a         1047 c          938 g         1177 t
ORIGIN      
        1 tcatccgtac atcattttct tgacataacg ggtaatgcag tttatctttg ccattctaat
       61 ttcagttggg caatagaaca atagaacaat agaaaataac aaataacctc aaaaacaatc
      121 attatgaaaa aacttttgac aattctactc agtgctacgg tgctttttgc ttttgcagaa
      181 gagcgtattg ccaataatcc gaaaccgatc aatcacggcc cgctggcttg ccccagcctg
      241 gtcatgaccc cggttgacaa taccgttacc tatcccagcg acctgatcga agcgatctta
      301 ggaccggatg tcgtctctta ttcgctcatc aacttcgccg ggttagccgg taatccttat
      361 gcatccgccg gtttattcgt aggcggaacc gatgccggat tagggatcga agacggaatc
      421 gtgatgtgct ccggatatat taccaatgct atcggaccca attctgatga tggagcatcg
      481 gttagtcttg gtcttcccgg agatgcccag ctatccgcat ttgccggaca atcaaccttc
      541 gatgccactg tcctcgaatt tagctttgtg ccgaatttcg accagcttta catccagtat
      601 gttttcggtt cggaagagta caatgaatat gttttccagt acaacgacgc ttttgcattt
      661 ttcctcaatg gcaacaacat cgccgtcgtc ccgagcacaa ccctcccggt aacgatcaat
      721 acgatcaacc tgtctaccaa taacacgtat tacaagaata atgactatgg cgatttatat
      781 cccggtccgt atccttattg caatgagatg gacggattta ctactgttct ggttgcacaa
      841 ggacccgtga acgaaggaga atccaatacc attaagctgg cgattgccga cggtggcgac
      901 tcagctctcg attcattctg ctttatcaaa ggatcgagct tcggcggaac cgacccggaa
      961 gtgccggtca gcaactgggc tttattcatt ggtatcggcc tgatcttaat ttttgccgtc
     1021 atccgtttca ggagactggt ttagtacaat aatagaatct ttaaatgaaa aaggctgccg
     1081 ggttttccag gcagcctttt tgtttgcaaa acgcaaaatg gaacatcagt gaataataaa
     1141 aaccttgaag gatctccctg tctctgaatc taacaggaaa tacaatccag accgcaaatg
     1201 tccaacattg gtgatcttct ctccttcaat ccgcataaca atcatgcctg tcgcgtttaa
     1261 aatgcagcca ttgaaaatat catcaaaata aatagtccca tttttaaccg gattcggata
     1321 aaatcgagtg ctttttacaa ccggctcagg ttcatctgtt ccaaagcttc cgttgctgta
     1381 tcccggcgta gcatggttaa aataaatcca ggaaagaaat ccgtcttctt ctctgccata
     1441 agatacatcg atcgggatca atccccagga aatactgtca attaaaaaat atcctgttga
     1501 ttcattgtcg aagatggcca gcgactcacc ttcatcgttc agtttataat cggtgtgaaa
     1561 cggcccctgt cccggctggc cgtcggtcca gatcaacaga aatccacctg gaggtattga
     1621 tacatctggc agtttccact tatcgggatt ggaaagatta tccgacaaat atttatcccc
     1681 catccaaacc ggatgatcat cggcattata aatctcgacc cagttatcat attcaccgaa
     1741 ttcatccgta atggttgtat cgttatcggc catcaattca ttgataaaaa tctgcggatc
     1801 atccgaagta tgaatatcaa taataaccgg agaacacggg cggaccgtga tcatcgattc
     1861 gggatcggtg accatcacct gccatgaaag gatctgtcca atgtcgaaac cgtcaaattc
     1921 atagccataa catccgtcgt cttcatcatg gtcatggtgt gttccgtcat ctttcatatt
     1981 caccagtaat tcagtccccc cgttaatact gtatttcagc ttcactgtaa aatcggccga
     2041 ctcatcggta accgtcgccc tgatccagta ttcttcaccg ggtaccgctt ttgatgattc
     2101 gatatagttg ataacaggag gaatgccatt gataaccaat tgctgcaagg ctgtcgtccg
     2161 ccgggtctga atataaggga tcaacccgta atcgacatga ccgcccagcg cctggtcgta
     2221 tgaatttatg aagttatcat acgtataacc ataatcgagc ggataaaacg gatcattcat
     2281 cacaaaaggg gcgatcattg tccgtattcc cccgatggcc gtaatcaaag aatcttcttc
     2341 agataaaaga tcgaggaatt ttttaaagaa gaaagaatac cggtctttga tcccgggtac
     2401 ctccagaagc cgtgcataca acggccttac ctccgtgcca tgttgctccc agtcgtagat
     2461 gtcgcgagtc gcccagtccc tatccatcca atcaattccc agcgtattat cgagatcgta
     2521 gggaatatac tcgaacctgc cggtttctgt attatggtaa aggtagaagt tattcttgtt
     2581 gtatatgtac ccgtcccagt tgccagtgat cacatccagg gccatgatct tcaggtagtc
     2641 gttgagatta aagacctttt ccagttcgca ttccagatct tccggcgggg tattgttcaa
     2701 aacatcaata aagtgagcaa gatcggagta attgttatat tcttcattga tcttcaattc
     2761 ataagcctgc cggccgtttt gataaaattt gtacagatcc ggatcattgc cgaggtaatc
     2821 gaggtcggcc ggccagaggc atttatacag gttcccgttt ttattgccga atcgcgactc
     2881 cgcgaattct tcatccacat gttcgaccat gatataaagc ccgtagtaat ctccgttgat
     2941 ataaaccgtg gcatgagcgg cccggggagc cgggacaccc gctgtacgaa gccactccca
     3001 gcaaatgcgc gaacgtatga tggaaggatc gttatgttca ccgttcaggt tcagtttctc
     3061 cattccctgg aactttttac ccggttcgaa tgtgttgaat gaaaccttaa atgatttctt
     3121 ctgcgaatac cgcgaagtat ttcccctaag cctgaatcct acattttcaa ccaattcatc
     3181 aatcgtctga ttttcaaaac ggaatgtagc atgagattcg atatctgaat ccacattttc
     3241 atagatccag gcaagcgtat cgggatcaat ggtaatatcg atccgtgcta cggttgaact
     3301 gtcgaaaacg ggagcgttag ggggaaactc gggttgggcg gtcagcgtgt tggtaaacca
     3361 cagagtcgga aagaagatgc agagtaccgc tggaaatttt ttcatcataa aacgatttta
     3421 atccttcaat aacagaaatg caggccaacc ctacttttcg gtataaacta ctttggtcac
     3481 cacatccccg acgacctgta aagttctttt atcaatctta tcgaaagtgt cttgtgttgt
     3541 atgccagtaa tcgtaaaaac taccgttagt ggagttggga tcaaggtgga taatgtcgat
     3601 ggcggggatt ttgcggatct cattgataaa atagtggtca tcgttaataa aagccccttt
     3661 ttcatcgcgg aaatattcct ggtatccaag gctggcggct atatcccaca ctttcttcac
     3721 cttatcgggg gcaaagtgca tggagaagcc ctctttcagg aagcgggcat ttccggctcc
     3781 caccatatcg aggaggatca caaaacgggc gttatacccc ggcacatgcg ggttatgcga
     3841 ccagaactgg gaaccaagac cccagtgctc attggccgcg ttctggctgt ctttcggcgg
     3901 gccatagtct tccaggtcga aaaagaggat atcgaccccg atgtcgggcg gatgattttt
     3961 aaagatccgg gccatttcaa gtaaaacacc cacaccgctg gcaccgtcgt tggctcccat
     4021 caccggcagg ttgtgtttgg ccaggtccgg atcataatcg gcataaggcc ttgaatccca
     4081 gtgtgcacag agcataatcc ggattttctt ctccgggttg aatgaggcga tgatattctt
     4141 ccctgtaaag gtctccccgg aataaacccg tgttttaaac tcctggaccc tgacatccgt
     4201 ggtataatcc atcaatttgg atctcagcca ggaagcgcaa tctgcatggg ctttgcttcc
     4261 cgggacacga ggcccgaacc cggtttgtgt tttgacaaaa tgaagagctg aatc
//
LOCUS       DAOQXS010000248         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20892_length_4302_cov_3.205321, whole genome shotgun sequence.
ACCESSION   DAOQXS010000248 DAOQXS010000000
VERSION     DAOQXS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20892_length_4302_cov_3.205321"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKM34_07600"
     CDS             complement(<1..230)
                     /locus_tag="PKM34_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83491.1"
                     /translation="MRKLLPALLLMAIVFSTGLFARKSVAQASDGAQLFQICGACHTI
                     GKGKLIGPDLYKVTERRDRAWLIPFIRNSQEV"
     gene            complement(431..1249)
                     /locus_tag="PKM34_07605"
     CDS             complement(431..1249)
                     /locus_tag="PKM34_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNQ83492.1"
                     /translation="MKRIFSLFLIGMILLSNVLTGQDIPEPMSPPRLVNDFAGLLNDN
                     QERLLEKKLVDFNDSTSTQIMVILVKSLNGMTKEEFADRVGEKWGVGQKGKNNGIVML
                     VKPRIGNESGQARISVGYGLEGAVPDGLTKRIVEMEMIPHFKNGDYYSGIDQAVNTLM
                     SLVKGEYTAEQYEKKQGGSIAGIIIPIIIIVLILIFMRGNSGRHYTTGSKGTSFWTAL
                     WLASMMNNRGGRGSWGDFRSGGGSFGGWGGGSGGGGFGGFGGGSFGGGGAGGSW"
     gene            complement(1260..1703)
                     /locus_tag="PKM34_07610"
     CDS             complement(1260..1703)
                     /locus_tag="PKM34_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNQ83493.1"
                     /translation="MVKTAKDFIDREQREDLRHAILNAELDTSGEIRVHIETTCKGDV
                     LDRAAFIFKQMGMHKTEKRNGVLFYLAVKNRRFAVIGDTGINAVVPDAFWDDLKHMML
                     NHFRENHFTQGLVEAIGIVGEKLKNHFPYQRGDVNELSDEISFGD"
     gene            complement(1729..2331)
                     /locus_tag="PKM34_07615"
     CDS             complement(1729..2331)
                     /locus_tag="PKM34_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ83494.1"
                     /translation="MNKSLVALIVVFVVLGLFVVIALLTAVPTYNKMVSYDEGVTSAW
                     SQVENVYQRRADLIPNLVNTVKGYAEFEKETLTQVIEARSKATSVNISAENLNEQTLS
                     QFQQAQDGLSSALSRLLVVVEKYPDLKANQNFLELQAQLEGTENRIANERMKFNDATK
                     TYNVYIRKFPARIFASLFGFDDKPYFKAAEGADKVPEVKF"
     gene            complement(2449..>3066)
                     /locus_tag="PKM34_07620"
     CDS             complement(2449..>3066)
                     /locus_tag="PKM34_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNQ83495.1"
                     /translation="PSQKDKDNKIIYLYASNKEEQHSFIQAATGRGYDVMIMDGILDN
                     HFINTLEHKLQDTLFRRVDSDTVDNLIKKEDQGISKLDDPQKETLKSIIEKNIEKEKF
                     TVVFENLSETEVPFLVVQPEFMRRMKDMSALGGGYMGLGNMPEFYNLVVNVNHPVMQN
                     ILDQQKDEEKDKIVSHLYDLALVSQNLLKGEKLAAFIKRSVEMMK"
     assembly_gap    3067..3166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3167..4182)
                     /gene="htpG"
                     /locus_tag="PKM34_07625"
     CDS             complement(<3167..4182)
                     /gene="htpG"
                     /locus_tag="PKM34_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNQ83496.1"
                     /translation="MQTGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLK
                     TLASVGSFKDELGDLTIKIEADKKAGTLTVKDRGIGMTAEEVDKYINQIAFSSAEEFV
                     KKFKTKSEAERNAIIGHFGLGFYSSFMVGKKVEVLTKTYKKGGQTKGVKWECDGSPDY
                     IMTESERKDRGTDVVVYIDDENKEFLEESRILEILKKYSRFLPVPIQFGMEKTWEKVE
                     GEKDEHGHDKEIQVEKPRIINNPDPLWKKKPTDCTEEDYKKFYRELYPMNFEDPLFQI
                     HLNVDYPFNLTGILYFPKVKRNVEIQKDKIQLYSNQVFVTDSVEGIVPEFLTLLHGVI
                     DSPD"
BASE COUNT         1038 a         1043 c          876 g         1245 t
ORIGIN      
        1 atcacttcct gcgagttccg gataaaaggg atcaaccatg ccctgtcgcg cctttccgtc
       61 actttatata aatcaggacc gatcagtttt cctttgccaa ttgtatggca ggcgccgcaa
      121 atctggaata gttgcgctcc atcggatgct tgtgcaaccg acttccgggc aaataaaccg
      181 gtggagaata cgatagccat gagcaacagg gcgggtagta atttgcgcat ggacaggcgt
      241 aaatatttat acatataaat actatttggt aaaacctgtt aagtgattga caccaataaa
      301 ttgatttaca gcgggcttgg taaatacaat aaaaaattat atccgcagta aattccgtgg
      361 ataaaaatag aaataaaatt atagaatgaa aagagggaga aaaaattaaa tcaggtacaa
      421 gtattaaacg ctaccagctc ccgccggcac cgccaccgcc gaatgagccg ccgccgaaac
      481 cgccgaaacc gccaccaccg cttccgccgc cccaaccgcc aaaacttcca ccgccactgc
      541 ggaaatctcc ccacgagccc cgaccgcccc ggttattcat catcgatgca agccacagcg
      601 cggtccaaaa ggaggtgcct ttgctgcctg ttgtgtaatg acgcccggaa ttgccgcgca
      661 taaagatcag tatgagcaca atgatgatga tgggtataat aattccggca atggaaccgc
      721 cttgtttctt ttcgtattgc tccgcggtgt attcaccttt taccagcgac attaacgtgt
      781 tgaccgcctg gtctatcccc gaataataat caccgttctt aaaatgaggg atcatctcca
      841 tttcaacgat ccgtttcgtc aagccatccg gtacagcccc ttccagcccg taacccacag
      901 agatgcgcgc ctggccgctc tcattgccga tgcgcggttt gaccagcatc acaatcccgt
      961 tatttttccc tttctgtcct acaccccatt tctcacctac cctgtcggca aattcctctt
     1021 ttgtcattcc gttaagagac ttgaccagga taaccatgat ctgtgtggat gtgctgtcat
     1081 tgaaatcaac cagtttcttt tcaagtagtc tttcctggtt atcattcagc agtcctgcaa
     1141 aatcgttgac cagacgcggt ggtgacatcg gttcggggat atcctgtcct gtcagaacgt
     1201 tgctgaggag gatcatccca ataagaaata aactgaatat acgtttcata ttaaacctgt
     1261 taatcaccga aactgatttc gtccgataat tcattgacat ctccccgctg gtatgggaaa
     1321 tgatttttaa gcttttcccc gacaatacct atcgcttcta ctagtccttg tgtaaagtga
     1381 ttctcgcgga aatggttgag catcatatgc ttcaggtcat cccaaaaggc atcggggacc
     1441 actgcgttaa tgccggtgtc gccgattacg gcaaaacggc ggtttttaac cgccaggtag
     1501 aagagaacac cgttgcgctt ctcggtcttg tgcattccca tttgtttgaa aataaaggct
     1561 gcccggtcaa ggacatctcc tttgcaggtg gtttcaatgt gaacccggat ttcccccgat
     1621 gtatccagtt ctgcattcag aatggcatgc cggagatctt ccctttgttc cctgtctata
     1681 aaatctttgg ctgttttgac catgtcgtga tccgtctgaa atgattcctt agaatttcac
     1741 ctcgggcacc ttgtcagcgc cctctgctgc tttgaaatat ggcttgtcgt cgaagccgaa
     1801 caaactggcg aatatcctgg caggaaattt acggatataa acgttgtacg ttttggttgc
     1861 atcgttgaat ttcatccttt cattggcaat ccggttctca gtgccttcaa gctgggcttg
     1921 cagttccaga aaattctggt ttgctttcag atcgggatac ttctcaacca caaccagcag
     1981 tctggagagg gctgaactca acccatcctg tgcctgttga aactgggata gggtttgttc
     2041 gttaaggttt tctgcagaaa tgttcaccga agtggctttc gagcgggctt caatcacctg
     2101 ggtcagggtc tccttctcaa attcagcata accctttacc gtgtttacca aattgggaat
     2161 caggtcggca cgacgctgat agacattctc cacctgcgac caggcagaag tgaccccttc
     2221 gtcgtacgaa accatcttgt tgtaagtggg cacggccgta agcaatgcga taactacaaa
     2281 caaccccagg accacaaaaa caacgatcaa tgcaactaat gatttgttca taatttctaa
     2341 atattaatat tttgctaaac tactaatttt cactttacta ttaatttttc actcttcaat
     2401 cttcactctt caatcttcac tcttcaatct tcactcttca cccttcactt atttcatcat
     2461 ctccacgctt cttttgataa atgccgccag cttttctcct ttaagaaggt tctgcgaaac
     2521 cagcgccagg tcgtataagt ggctgacgat tttgtctttt tcttcgtcct tctgctgatc
     2581 aaggatgttc tgcattaccg ggtgattcac atttacgacc aggttgtaaa actccggcat
     2641 atttcccaaa cccatgtatc cgcctcccag ggccgacata tctttcatcc tccgcatgaa
     2701 ttccggctgg acgaccagga aagggacttc cgtttcactt aggttctcaa agacaacagt
     2761 gaacttctcc ttttcaatat ttttttcaat aatgctcttc aacgtctctt tttgcggatc
     2821 atccagtttc gaaattccct ggtcttcttt tttgatcagg ttgtccactg tgtcggagtc
     2881 gacgcggcgg aaaagggtgt cctgcaattt atgttcaagc gtgttgatga aatgattatc
     2941 gaggatgccg tccatgatca tgacatcgta tccccggccg gtagccgcct ggataaagct
     3001 gtgctgttct tccttattgc tggcataaag atagatgatc ttgttgtctt tgtctttctg
     3061 cgacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt cgggcgagtc
     3181 gatcacaccg tgcaataatg taagaaactc aggtacaatt ccttcaacgg agtcggtaac
     3241 aaagacctgg ttagaataaa gctggatctt gtccttctgg atctcgacat tacgcttcac
     3301 tttggggaaa taaagaattc cggtgagatt gaaagggtaa tcaacattga ggtggatctg
     3361 gaaaagggga tcctcaaagt tcatgggata aagttcccgg taaaactttt tgtaatcctc
     3421 ttcggtgcaa tctgttggct ttttcttcca gaggggatcg ggattattga tgatcctggg
     3481 cttctcaacc tggatctctt tgtcgtgccc atgttcatct ttctcaccct ccactttttc
     3541 ccaggtcttc tccattccga actggatcgg taccggcagg aaccgggagt atttcttcaa
     3601 tatttcgagg atccggcttt cctccaaaaa ctctttgttc tcgtcatcaa tataaacaac
     3661 cacatccgtc ccgcgatctt tccgttcgga ttcggtcatg atataatcgg ggctgccgtc
     3721 gcattcccat tttacccctt tggtctgtcc gcctttctta taagtcttgg tcaggacttc
     3781 gacttttttg cccaccatga acgaagaata gaaacccaga ccgaaatggc cgatgatcgc
     3841 attccgctct gcttcacttt tcgttttgaa cttcttgaca aattcctcgg cgcttgaaaa
     3901 agcaatctgg tttatatatt tatctacctc ctcggcggtc ataccaatgc cccggtcctt
     3961 aacagtcagg gtccctgcct ttttatcggc ttcgattttg atggtgagat cgccgagttc
     4021 atctttaaat gaaccgaccg aagccagggt ctttaatttt tgtgttgcgt caaccgcatt
     4081 ggaaaccagc tcccttaaaa aaatctcgtg atcggaataa agaaatttct tgataatggg
     4141 aaaaatgttt tcagtctgaa cgttgatctt tccggtttgc ataatgataa gattttggcg
     4201 gtttttttat caaagtgtat accgaaatca gtagctgaca ttttgacggg gaatattttt
     4261 gtctgccggt tgctgaaaaa tttctaattt agccctgtta aa
//
LOCUS       DAOQXS010000249         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20902_length_4300_cov_4.136631, whole genome shotgun sequence.
ACCESSION   DAOQXS010000249 DAOQXS010000000
VERSION     DAOQXS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20902_length_4300_cov_4.136631"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1304
                     /locus_tag="PKM34_07630"
     CDS             30..1304
                     /locus_tag="PKM34_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNQ83497.1"
                     /translation="MQQKLNPQDIEIMAPVGSYESLSAAIQGKADAVYFGIGHLNMRS
                     RSTQNFSLEDLAEIAAICRENHLRSYITLNTEIFDEEISLMRAIVDEAKKAGITAIIA
                     SDQAVIQYARSREVEVHMSTQANITNTEAVKYYAQFADVMVTARELNLDQVKRITENI
                     EGERIIGPSGKLVQIEVFAHGALCMAVSGKCYLSLDNMNSSANRGACLQLCRRPYRVF
                     DRDGEVELEVDNQYIMSPKDLKTIDFIDKILDAGVRVLKIEGRGRSPEYVKTVTRCYR
                     EAVNAHCDGTFNPENLERWNHELGTVYNRGFWDGYYLGRTVGEWAAGYGSQATKKKIY
                     VGKVMNYYARNGIAEVQIEAHPLRKGDHFLVLGPTTGVAEGITPDIWLEEKIVGEVNR
                     GDLCTFPFDTPLRPSDKLYKWVDSREVEGQID"
     gene            1450..2298
                     /locus_tag="PKM34_07635"
     CDS             1450..2298
                     /locus_tag="PKM34_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNQ83498.1"
                     /translation="MMKYFNLHTHCNYCDGAGFPEEYVNAAIKKQFHTLGFSSHAPVP
                     FKNTFAIRDEQELQKYCDQIIHLKEIYKDKIDIYLGLELDYIEGISGDFGKIKEKYGL
                     DYTIGSVHLVRNGDDENLWFIDGPRVESYDEGLKSVFKGDVKQAVTTYYEQVKKMVVT
                     QKPDILGHFDKIKMHNKDRYFKEDEAWYRDRVMDLLDAISTTGVIPEVNTRGIYKKRS
                     SDLYPGVWILKEMKKMNIPVILSTDAHHPVEIDGYFDETVGILKEIGYNSLVCYNANG
                     WQELPI"
     gene            2771..3199
                     /locus_tag="PKM34_07640"
     CDS             2771..3199
                     /locus_tag="PKM34_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83499.1"
                     /translation="MKNLMKTLVLISVLFIAGMQNINAQLSIKVILDGQCPDTCTSHE
                     ICTYKVEYQLYNICVEPPELCCTAPPQWVDCSTEEIYFNCDFSCDDPSNEKCYALVGK
                     ATKYCNGPGGNIAICVGTGIAYYTCHEVMTLSPLPVTVYW"
     gene            3266..>4300
                     /locus_tag="PKM34_07645"
     CDS             3266..>4300
                     /locus_tag="PKM34_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83500.1"
                     /translation="MRIPFTILQILITVKFIIAQNETNIWYFTKGCGLDFNSGNPEVL
                     YDGQAIHGWASSTMCDSSGMFLFCADAEYIYSVYGIMQNSDGLVNPRIAGLASVKWPE
                     QEFLYFLFTAADPNIPNAGFYYSLVDLSQGLGGVVIEKNIPIPSGWDVSDRIAIVKKE
                     KSDNVWIIVRKNEEDAIAAYLLDEIGFHADPVISTMPNLEMTWRSIWGYLKISTNKSF
                     LISTYGSYGFEVCSFDAANGVAVHLFLADSPSPNSPSCSGVEFSPDSKYLYVNYTGQA
                     SSELYQYDMQYVFDSASFKASALKIDSYNSFELQLARDGKIYCSPNQVLDQTNWYNIS
                     VIEKPWIRGEG"
BASE COUNT         1249 a          854 c         1013 g         1184 t
ORIGIN      
        1 atacctcaat tgattgtacc tttgcagcca tgcagcaaaa actgaaccct caggatattg
       61 agattatggc cccggtgggg agctatgaat cgctttcggc cgccatacag gggaaagccg
      121 atgcggttta cttcggcatc gggcatctga acatgcgctc gcggtcgaca cagaatttct
      181 ccctggagga tctggcagag attgctgcga tttgccgcga aaaccacctc cgctcttaca
      241 ttaccctgaa taccgaaatt ttcgacgaag agatttccct gatgcgggcg atcgtggatg
      301 aagcgaaaaa agcggggatc acagcgataa tcgcctccga ccaggctgtg atccagtatg
      361 cccggagccg ggaggtagag gtgcacatgt cgacccaggc caacatcacc aataccgaag
      421 ccgtaaagta ttacgcgcag tttgccgatg tgatggttac agcgcgggaa ctgaacctgg
      481 atcaggtgaa gagaattact gagaacattg aaggagagcg gattataggt ccttcgggga
      541 agctggtgca gattgaggtg tttgcccatg gcgctttatg catggccgtt tcgggcaaat
      601 gttacctgag tctcgacaac atgaattcct cggccaaccg cggcgcctgc ctgcagctct
      661 gccgccggcc ttaccgggtc ttcgaccgcg acggcgaagt ggagctggag gttgataacc
      721 agtacatcat gtcgcccaaa gacctgaaaa cgattgattt tattgataag atcctggatg
      781 cgggagtgag agtgctgaag attgaaggcc gtggccgctc tcccgaatat gtgaaaaccg
      841 tcacccgatg ctaccgcgaa gccgtcaatg cccactgcga cggtacattc aatcctgaaa
      901 acctggaacg ctggaaccat gaacttggaa ccgtttataa tcgtggcttc tgggacggct
      961 actacctggg gcggacagtg ggcgagtggg ccgccggcta tggctcgcag gctacaaaaa
     1021 agaagatcta tgtggggaag gtcatgaatt attatgccag gaacgggatc gcggaggtgc
     1081 agatcgaggc gcatccgttg cgcaaaggcg atcatttcct tgttctcggg ccaacgaccg
     1141 gcgtggccga agggattacc ccggatattt ggctggaaga gaagatcgtg ggcgaagtga
     1201 accgcggcga cctgtgcact tttcctttcg atacgcctct ccggccttcc gataaacttt
     1261 acaaatgggt cgacagcagg gaagtggaag ggcagattga ttaaaagcgt gattaaaaag
     1321 gaactgcccc ggatgggaac cggggcagtg tacatttttg ccaaacctaa gggagagaat
     1381 gttagtcttc taacgactat taatcggatt tttgtgataa ggtaatttat tttaacgatt
     1441 tttttattga tgatgaaata cttcaatctt catactcatt gcaattactg tgatggcgcc
     1501 ggtttcccgg aggaatatgt taatgcggct attaagaaac aatttcatac cctgggattc
     1561 agcagtcatg cgccggttcc atttaaaaat acttttgcta taagagatga gcaggaactt
     1621 cagaaatatt gtgatcaaat cattcatctt aaagaaattt ataaagataa aattgatatt
     1681 tacctgggat tggaactcga ttatattgaa gggatcagcg gggattttgg taagataaaa
     1741 gagaaatacg gtctcgatta cacgattgga tcggtccacc tggtaagaaa tggagatgac
     1801 gaaaacttat ggttcatcga cggcccgagg gtggagtcgt atgatgaagg gttgaaaagt
     1861 gtttttaaag gtgatgtaaa acaggcagtg acaacctatt atgagcaggt taaaaagatg
     1921 gtcgtcacac agaagcccga tatcctgggg catttcgaca agataaagat gcataacaag
     1981 gaccggtatt ttaaggaaga tgaggcgtgg taccgcgacc gggtgatgga tttgctggat
     2041 gcgatatcaa caacgggtgt gatccccgag gtcaataccc gcggtattta caaaaagcgg
     2101 agcagcgatc tttatcccgg ggtatggata ctcaaggaaa tgaagaagat gaatatcccg
     2161 gtaattctca gcaccgatgc ccatcatcca gtcgagatcg acgggtattt tgatgaaacg
     2221 gtcggaattc tgaaggaaat cggctacaat tcgttggtct gctacaatgc aaatggctgg
     2281 caggaattac ccatttaaat cgaatatcgc ccgttcgcct gttcgtgatt tcggctgatg
     2341 attaagatcc ctccttcatc gggatgacgt catcccgagc gtagcgaggg atccgccgac
     2401 cgccgacctg ttcataactt tccccgaaaa catatattac tttttcctat atttaacgat
     2461 caattgccag attaaccaac tatgtccctg acatatacat atcgtgaata tttttattct
     2521 ctgaagtttt tcttaatttg ccgccccgct agtcttcttt ctttcttacg aaaacaggac
     2581 tttccagaga ttagtttctt ctctcaattt cgaaaattcc catcgaacat cagcaatcga
     2641 acatcggcaa tcgaatatcc atttctcaca ctcccatcct ccatgagggg tttgttaccc
     2701 caaaagtgaa aaagaattaa ccagaaaact ccacctccaa aaaatagtat taattttaaa
     2761 tctgagtctt atgaagaatt tgatgaaaac tttggtgtta atttcggtat tattcatagc
     2821 cggaatgcaa aatataaatg ctcaactaag cattaaagta atattggatg gtcaatgtcc
     2881 tgatacgtgt acttctcatg aaatttgcac atataaagtt gaatatcaac tgtacaatat
     2941 ttgcgtcgaa ccaccagaac tttgctgcac tgcacctccg caatgggtgg attgttctac
     3001 ggaagaaata tattttaatt gtgatttctc atgtgatgat ccttcaaatg aaaaatgtta
     3061 tgcattggtt ggtaaagcaa ctaaatactg caatggacct ggcggaaata tagcaatatg
     3121 tgtaggaact ggaattgcat attacacatg tcatgaagta atgacattaa gtccattacc
     3181 tgtaacagtt tattggtgat aattaaaatg aatatctgtc cggcaagatg aaacgccgga
     3241 cagattcttc aaatacttta ataagatgcg aattccattt acgattcttc aaatattgat
     3301 cacagttaaa tttattattg cacaaaacga aactaatata tggtatttta cgaaaggctg
     3361 tggcttagat ttcaattctg gaaatccgga agttctttac gatggacagg ctatacatgg
     3421 ttgggcaagt tcaacaatgt gcgactcttc aggaatgttt ttattttgtg cagatgccga
     3481 gtatatttat tcggtttatg gtataatgca aaacagtgac ggattggtca accctcgcat
     3541 tgcaggttta gcttcagtca aatggcctga acaagaattc ttgtattttc tatttacagc
     3601 tgcagatcct aatataccca atgcaggatt ctattattct ttggttgatc taagtcaagg
     3661 ccttggagga gtggttattg aaaaaaatat tccgattcct tccggttggg atgtaagtga
     3721 tagaattgct atagtaaaaa aggagaaatc agacaatgtc tggatcatag tcagaaaaaa
     3781 tgaagaagat gcaattgcag cttacctatt agatgaaatc ggattccatg ctgatcctgt
     3841 tatttcaaca atgccaaatt tagaaatgac ctggcgaagt atatgggggt atcttaaaat
     3901 ctccacaaat aaaagttttt tgatttccac atatggcagt tatggctttg aagtctgcag
     3961 tttcgatgct gcaaacggag tggctgttca cctttttcta gcagatagtc ccagccctaa
     4021 ttcgccgtct tgttcagggg ttgagttttc acctgattca aaataccttt atgtaaatta
     4081 tacgggacaa gcttcaagtg aattgtatca atatgatatg caatacgtgt ttgattcggc
     4141 ttcatttaaa gcgtctgctt taaaaataga cagctataat tcatttgaat tgcaattggc
     4201 aagggacgga aaaatttatt gttcaccaaa tcaggttttg gatcaaacaa actggtataa
     4261 tataagtgtc atagagaagc cttggattag gggtgagggt
//
LOCUS       DAOQXS010000250         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20926_length_4297_cov_4.068600, whole genome shotgun sequence.
ACCESSION   DAOQXS010000250 DAOQXS010000000
VERSION     DAOQXS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20926_length_4297_cov_4.068600"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="PKM34_07650"
     CDS             complement(<1..2241)
                     /locus_tag="PKM34_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83501.1"
                     /translation="MNLLFRAFLLILTAVCFITATNAQITPQVRRAVHSDKTPPLREM
                     RLIEPQPRDRSWKNGIIRNEDSGRKRNENPSPLPSGLDPALQSEFGFRDFPGMVANFE
                     GVPNRNSVYPPDTDGDVGPNHYFQMINLSFQIFDKQGNSLYGPADNSTLWSGFIGSWT
                     GTNDGDPILLYDEMADRWVATQFAINTSDGTYWELVALSESGDPLGAYYRYAFQFPAF
                     NDYPKVGVWPDGYYFSFNMFGNDYRRAAVCALDREAMLNGDPGARMVLFDMPQGSEPW
                     SLLPADFDGPPPPAGSPNYFIYAMDDAFGSGDYLSLWAFVTNWEAPENSTFQEMGRLD
                     TEPFNSFFCEASLGACLPQPDNGPNLEVLSDRLMYRLQYRNFGTYQTMVTNHTVNADG
                     QGHAGIRWYELRNNNDGNWFIYQQGTYAPDENDRWMASVAMDGRGYIALGFSIVSPVM
                     YPSIRYTGRSPNDPLGQMTFFEEVIMAGTGIQTGSAARWGDYSMMSVDPADDTTFWFT
                     TEYVKNSGQVSWKTRIAGFRMVEDNLAPADVLDLQATANTTNSITLRWTATGNDGNNG
                     TAFLYDIRYSKVPITDENFDAAIQAENEPVPSESGSQEVFTINDLEFSTTYYLAMKVR
                     DRQFNFTGLSNTVQALTPGKPVMAVEETAIVQKIFPGSAGQREFIIRNTGESDLCFKT
                     QKEAAGGIKHPRNLGDYADRSRQSDPDADAWVREALSNDGLSGSRAAGDILGEYTGAT
                     SSISGMT"
     gene            2447..4003
                     /locus_tag="PKM34_07655"
     CDS             2447..4003
                     /locus_tag="PKM34_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNQ83502.1"
                     /translation="MTIQAQDQPGSGTAMKGAFVDQAGSPVVLLGDTLFTIHSAYGAF
                     SAGDRAAAISDKLMKVVKEPVFYADSLTGFYVESDYYIVYRNKVLMVIQPADTAGTGM
                     TQDEISGRVFNLLNEELAAKSAKFNITATVTNIAYSLIVVFVLVLIIWLLNWLFRKII
                     KYAEKHKERLFKSIKINEYEFLTAEREFVVARGLLRFFKIVLIIILFLVALPVIFSIF
                     PSTEGITRKIIAMIWEPIQNILMGIIGYLPKLITIIIIYLLFKYLIRLIRYLTKEVEN
                     KALVIKGFHPEWALPTFNIVRILLYAFMFIIIFPYLPGSDSPIFQGVSVFLGLLISLG
                     STSLIGNTLAGLVITYMRPYKVGDRITIEDITGDVIEKTLMTTRLRTIKNEEVTIPNS
                     KILTGYTVNFTAATLDEGLIIHTTVTIGYDAPWRKVHELLIRAAEKTAGVAVQPKPFV
                     LQTGLEDFYISYQINAYVKNSHGIVRIKSDLHQNIQDEFNKAGVEIMSPHYRAERDGN
                     KVTIPENSEE"
     gene            4118..>4297
                     /locus_tag="PKM34_07660"
     CDS             4118..>4297
                     /locus_tag="PKM34_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83503.1"
                     /translation="MGFLPVWMFGQAALKPGENLAGIMLPAGYKIEVYTDQVPNARAM
                     DFAEDGTLFVGSMVEG"
BASE COUNT         1002 a          985 c         1223 g         1087 t
ORIGIN      
        1 tgtcatcccg gagatggagg aggtggcccc ggtgtattcg cccaggatat cgccggctgc
       61 gcgggatccg ctcaggccgt cattcgacag ggcttcgcgc acccaggcgt cagcatccgg
      121 atcggattgc cggcttcggt cggcataatc gcccaggttt cggggatgtt ttattccgcc
      181 cgcggcctct ttctgagtct taaaacagag atcgctctca ccggtattcc ggatgatgaa
      241 ttccctttgc ccggcggagc ccgggaatat cttctgaacg atagccgttt cctccaccgc
      301 catcacgggc ttgcccgggg tcaaggcctg caccgtgtta gaaagcccgg tgaaattgaa
      361 ctggcggtcg cggaccttca tggccaggta gtaggtggtg ctgaactcca gatcatttat
      421 ggtaaacacc tcctgggagc cggattcaga ggggacaggc tcgttctcag cctggatggc
      481 ggcatcgaag ttttcatccg tgatgggcac tttggagtag cggatgtcgt agaggaaagc
      541 cgtaccgttg tttccgtcgt taccggtggc tgtccatcgg agcgtgatgg agttggtagt
      601 attggcagta gcttgcaggt cgaggacgtc tgccggcgcc aggttgtctt ccaccattct
      661 gaatccggct attcgtgttt tccagctcac ctggccggag ttttttacgt attcggtagt
      721 aaaccagaat gttgtatcgt cggccggatc gaccgacatc atgctgtagt cgccccagcg
      781 tgccgcgctg ccggtctgga taccggttcc ggccataatt acttcttcaa agaaggtcat
      841 ctgacccagg ggatcgttcg ggctacggcc cgtataccgg atcgaagggt acatgaccgg
      901 gctgacgata ctgaatccca gggcgatata cccccggccg tccatggcaa cactggccat
      961 ccagcggtcg ttctcatcgg gggcataggt gccctgctga tagatgaacc agttgccgtc
     1021 gttgttattc cgcagttcgt accagcggat accggcatgt ccctgtccgt cggcattgac
     1081 cgtatgattg gttaccattg tctgataggt gccgaagttc ctgtattgca ggcggtacat
     1141 cagcctgtcg gacagcacct ccaggttcgg cccgttgtcg ggttgcggca ggcaggcgcc
     1201 caaagaggct tcgcagaaga atgagttgaa aggttcggta tccaggcggc ccatctcctg
     1261 gaatgttgag ttttcagggg cttcccagtt cgttacgaaa gcccacaggc tcaggtagtc
     1321 gccgctgcca aaggcatcat ccatggcgta gataaagtaa ttcgggctgc cggcgggtgg
     1381 cggggggcca tcgaaatcgg ccggcaggag gctccagggt tcgcttccct gcggcatgtc
     1441 gaaaagcacc atccgggcac cggggtcgcc gttcagcatc gcttcgcggt cgagagcgca
     1501 tacggctgcc ctgcggtagt cgttgccgaa catattaaag gaaaaatagt agccatcggg
     1561 ccagacgccc actttcggat agtcgttgaa agccgggaac tggaaagcat agcggtaata
     1621 ggcccctagc ggatcacccg attccgagag ggcgacaagc tcccagtaag tgccgtcgga
     1681 agtattgatg gcaaattggg tagctaccca gcgatcggcc atttcgtcgt acaacaggat
     1741 cgggtcgccg tcgttggtcc ctgtccagga gccgataaac ccgctccaga gggtggaatt
     1801 gtcggcaggc ccgtagagag aatttccctg cttgtcgaag atctggaacg acaggttgat
     1861 catctggaaa tagtggttgg ggccgacatc tccgtcggta tcgggcgggt agactgagtt
     1921 ccggttcgga acgccttcga agttggccac cattcccgga aaatcccgga atccgaattc
     1981 tgactggagt gcaggatcca accccgacgg caggggagaa ggattttcgt ttcgtttccg
     2041 gcccgagtcc tcattgcgga taattccgtt cttccagctt cggtcgcgag gctgcggttc
     2101 gatcaaccgc atttccctga gcgggggtgt tttatcggaa tgaacggccc ggcggacctg
     2161 aggggtgatc tgggcgttag tcgcggtaat aaagcaaacg gctgtaagta tcaggagaaa
     2221 tgctctgaat agtaaattca taatacagtt ttggttaaat gatgaccagg agtttttcct
     2281 gaaatcgact atcaaaagta ggaggaaaaa gagttggtat tgagatggag tctcaaaaat
     2341 tgagactcca tctcaatttt atgtaacttt gttatataac caagggctta gtttacatat
     2401 gaagatagtc aggataatca taactgcggt tgccgtaata tcagctttga caattcaagc
     2461 acaggatcag ccgggcagcg gaaccgccat gaagggtgcc tttgtggacc aggccggtag
     2521 tccggttgta cttcttggag atacattgtt cacaatccat tctgcctatg gcgctttttc
     2581 agccggagac cgcgcggctg ccatatcgga taagctgatg aaggtggtga aggagcctgt
     2641 tttttatgct gattcgctga ccggtttcta tgttgaaagc gattattaca ttgtttaccg
     2701 caacaaggtg ctgatggtta ttcagccggc ggataccgcg gggacaggta tgacacagga
     2761 tgaaatttca ggtagggtgt tcaatttgct gaatgaggaa ctggcggcga agagcgcgaa
     2821 attcaacatc acggcaactg ttactaacat tgcttactcg ctgattgtgg tctttgtcct
     2881 ggtattgatc atctggttat taaactggtt attcaggaag ataataaaat atgcagagaa
     2941 gcataaagaa cgtcttttca aaagcatcaa aataaatgaa tatgagtttc ttacggcgga
     3001 gcgggagttt gtggtagcca ggggattgtt gcggtttttt aagattgttt taataataat
     3061 cctgttcctg gtagccttgc cggtgatttt cagcattttc ccatcaaccg aggggattac
     3121 ccgaaagatt atcgccatga tctgggagcc gatacaaaat atcctcatgg ggatcatcgg
     3181 ttatcttcca aaattgataa ccatcattat tatctactta ttattcaaat acctgatccg
     3241 gcttatcagg tatcttacga aggaagttga aaataaagca ttggtaataa aaggatttca
     3301 tcccgaatgg gccctgccga cttttaatat cgtccgcatc ctgctctatg ctttcatgtt
     3361 catcatcata ttcccatacc ttcccggttc cgattccccg atattccagg gggtatcggt
     3421 tttcctggga ttgcttattt cgctgggttc cacttcactg atcggaaaca ccctggccgg
     3481 gctggtgatc acttacatga ggccttataa agtgggcgac aggatcacca tcgaagatat
     3541 tacgggtgat gttattgaaa agaccctgat gacgacaagg ctcagaacaa ttaagaatga
     3601 agaagttaca attcccaatt caaagatatt gaccggttat acggttaatt tcacagcggc
     3661 aacgctggat gagggtctta tcattcacac gaccgtgacg attggctacg atgccccgtg
     3721 gcggaaggtg catgagcttc tgatccgggc tgcggaaaag acggcggggg tggctgttca
     3781 accgaaacct tttgttttgc agaccgggct ggaggatttt tatatcagct accagatcaa
     3841 cgcttatgtt aaaaactctc atggcatcgt aagaatcaaa tcggacttgc atcagaacat
     3901 acaggacgag ttcaacaagg cgggggttga gatcatgtcg ccgcattatc gcgccgagcg
     3961 ggatgggaat aaggtgacga ttccggagaa tagtgaagag tgaaaagtga agagtgaaaa
     4021 gtgaagagtg aaaagggaat ggttttggga tttgattttg ggatgattag aatttattca
     4081 ggatatgagg ataatagtta gtttattttt tggatggatg gggtttttgc ctgtgtggat
     4141 gtttggacag gcggccctga aaccgggtga aaatcttgcc gggatcatgt tgccggcggg
     4201 ttacaaaata gaggtttaca ccgaccaggt gccgaatgcc cgtgccatgg attttgcgga
     4261 agacgggacg ctgttcgtcg gttcgatggt agaggga
//
LOCUS       DAOQXS010000251         4288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_20985_length_4288_cov_3.361682, whole genome shotgun sequence.
ACCESSION   DAOQXS010000251 DAOQXS010000000
VERSION     DAOQXS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20985_length_4288_cov_3.361682"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKM34_07665"
     CDS             <1..335
                     /locus_tag="PKM34_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HNQ83504.1"
                     /translation="SMRHSARNAGLGILGRNTLLINEEMGNIVYIGAVLVDAILEADP
                     LVTDFHCPPHCRLCIDACPVGAFDGVTVIQERCRKHSIASHPRGWSLYTCSACRAACP
                     YAEGTGKG"
     gene            complement(409..1152)
                     /locus_tag="PKM34_07670"
     CDS             complement(409..1152)
                     /locus_tag="PKM34_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ83505.1"
                     /translation="MIIKEIRAKTILSASKIYDYVINPYVGCEHGCSYCYARYIKKFT
                     GHYEPWGGFVDVKVNAPELLKKEILKKKKAMVWISGLCDPYQPPEAHYELTRQCLEVL
                     AFYDWPVSIQTRSALVLRDLDMMNKFSELQAGFSIPTADDSVRMIFEPHATPVGDRIS
                     ALEELHRHGITTYAMIAPVLPGAENLVSLLAGKVDHILVDRMNYRYAEGIYKKHGLES
                     KMTDDYFAETGMLISEQCRKAGIACRVVF"
     gene            complement(1203..3629)
                     /locus_tag="PKM34_07675"
     CDS             complement(1203..3629)
                     /locus_tag="PKM34_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ83506.1"
                     /translation="MFRILLKYSFRSLSRHRAHVAINITGLLVGMVCSMIIALFIIHE
                     LSYDRFNEKKDRIYRVILNGKLGGQEVKVTYTASPIGPTMKNEFPEVEDFLRMNNWGE
                     TIIRYGERNFTEKDFIEADSSFFGFFSVPLIRGDAKEVLNEPHTLVLSESTAKKIFGE
                     EDPIDRMLKVSTDTVPYRVTGIMEDIPGTSHFAANIISSFMTNPRAGDNEWLSNSFST
                     YVLLHPGASPQSAEARIPDMIVKYVGPILQSFLGVTVEDFFAQGNKYSMYLQPLAKVH
                     LDPSIEQAFKRANDPKYLMIFGSIGILIILIAAFNFMNLATAQAARRAREVGIKKVSG
                     ATRNQLISQFLGESVILSLIALILAVAVTLLVLPHFNTLLDIEMKADLTGTWYTIPLL
                     LFISIFIGIMAGSYPAFYLSSFNPYMVLKGSTVRGHNSGKLRNLLVVLQFAISIILIV
                     GTTIMFRQLHYMQNKALGFDKENVMVISRADAIGNHIGSFKDELLKIPGVMNVAASTA
                     VPGHNNNNNGYRVQGRPEESYLLQTNWVDYDYLDVYGIKLASGRFFDKSYATDKEACV
                     INQCAVNHYRFADPFGIRFIVPTGSNPEDVEVRPVIGVVNDFHFESLRTRIGPYIMHF
                     KDERNNWGYVSLRLGPETGPSTLAEIEKVWASYTSNDPLQYFFMDKDLERLYKEERQN
                     SKLAVMFTILGILVASLGLFGLTSYTVQQRTKEIGVRKTFGASVYNIWYMIAREILLL
                     ITISAVLAIPFAWWIAGNWLQNYEYRISLQVFDFLAGFLIAIVIALMTISYRAIRTAQ
                     MNPVVALRYE"
     assembly_gap    3768..3867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          953 a         1071 c         1141 g         1023 t
ORIGIN      
        1 tgtcgatgcg gcacagcgcc cgcaacgccg gcctcggaat actgggaagg aatacactgc
       61 tgatcaacga agagatgggc aatatcgttt acatcggcgc tgtccttgtc gatgccatcc
      121 tggaggccga tccgctcgtc acagattttc actgcccgcc ccactgccgg ctttgtatcg
      181 acgcctgccc cgtcggcgcc ttcgacggtg ttaccgtgat ccaggaacgc tgccggaagc
      241 attccatcgc cagccacccg cgcggctgga gcctttatac ctgctcagcc tgcagggcgg
      301 cctgtccgta tgcggaggga acggggaagg gctgataaag tgtatcctgt tgttgtaatg
      361 gtagatgatt tgatctttca ggaatcattc tgaaaattat tctgaaggtc aaaaaaccac
      421 cctgcatgct attcctgctt tccggcattg ctccgagatg agcattcctg tttcagcgaa
      481 ataatcgtcg gtcattttgc tttccagccc atgtttctta tagatcccct cggcatagcg
      541 gtaattcatc cggtcgacaa ggatatggtc gacttttccg gcgagcaggg acaccaggtt
      601 ttccgccccg ggcagtaccg gtgcgatcat cgcataggtg gtgatgccgt gacggtgcag
      661 ctcctccagt gctgaaatcc gatctccgac cggcgtagcg tgaggctcaa agatcattct
      721 gaccgaatca tcggcggtgg ggatggaaaa gccggcctgc aactcactga atttattcat
      781 catatccagg tctctcagta ccagcgccga ccgggtctgt atcgatacgg gccagtcgta
      841 gaaggccagc acctcaaggc attgcctcgt cagttcgtaa tgtgcttcgg gcggctggta
      901 aggatcgcac aaaccgctga tccagaccat ggctttcttt ttcttcagta tctctttctt
      961 gagcaattcc ggtgcattga cctttacatc tacaaacccg ccccacggct cgtaatgccc
     1021 cgtgaacttc ttgatataac gggcatagca gtaagaacat ccgtgttcgc atccgacata
     1081 cggattgatc acatagtcgt agatctttga tgctgaaagg attgtctttg ccctgatttc
     1141 tttgataatc atgggttaaa ggtaattaat tccggtcgtt ggtcgttttt cccttttccc
     1201 tttcactcgt accttagcgc taccaccggg ttcatctgcg ccgtcctgat cgcacggtag
     1261 ctgatggtca tcagggcgat aacgatggcg atcaggaagc cggccaggaa atcgaacacc
     1321 tgcaagctga tccggtattc gtagttctgc agccagttgc cggcgatcca ccaggcaaac
     1381 gggatggcca gcacggccga gatcgtgatg agcaaaagta tctccctggc gatcatgtac
     1441 cagatgttgt acaccgaggc cccgaaggtc tttctcacgc cgatctcttt ggtgcgctgc
     1501 tgtacggtgt aggaggtgag cccgaaaagc ccgagcgacg cgaccaggat gccgagaatg
     1561 gtgaacatga cggcgagctt cgagttctgc cgttcttcct tatagagccg ctccaggtcc
     1621 ttgtccatga agaagtactg taggggatcg ttagatgtgt atgaggccca taccttctca
     1681 atctccgcca gcgtcgaagg accggtctca gggcccaggc ggaggctcac atagccccag
     1741 ttgttccttt catctttaaa atgcatgata taaggcccga tccgggttcg cagcgattcg
     1801 aagtgaaaat cgttcaccac gccgataacc ggcctgacct cgacatcctc cggatttgac
     1861 ccggtcggga cgatgaatct gatgccgaag ggatcggcga accggtaatg gttcacggcg
     1921 cactggttga tgacacaggc ctctttgtcg gtggcgtagc ttttatcgaa gaaccggccc
     1981 gacgccagtt tgatcccgta aacatccagg tagtcgtagt cgacccagtt ggtctgaagc
     2041 agatacgact cttcgggccg cccctgcacc cggtagccgt tgttgttgtt gttgtgaccc
     2101 ggcacggcgg tggaggctgc aacattcatg acgccgggaa ttttcagcag ttcgtccttg
     2161 aaactgccga tatggtttcc gatagcatcg gccctgctga tcaccatcac attttccttg
     2221 tcaaagccca gcgctttgtt ctgcatgtag tgcaactgcc tgaacatgat agtggtgccg
     2281 acgatcagga tgatggagat ggcaaattga agaaccacca gcaggttcct cagctttccg
     2341 ctgttgtgtc ctctcaccgt gctgcctttc agtaccatgt aagggttgaa ggacgacagg
     2401 tagaatgccg ggtagcttcc ggccatgatc ccgataaaaa tggagatgaa cagcagcagc
     2461 gggatggtgt accaggtgcc cgtcagatcc gctttcattt cgatatccag gagcgtgttg
     2521 aaatggggga ggaccaggag cgtaacggcc acggccagta tcaatgcgat aagcgaaagg
     2581 atgacacttt cgcccaggaa ctggctgata agctggtttc gcgtggcgcc gctgactttc
     2641 ttgatcccga cctcccgggc acgccgggcc gcctgtgccg ttgccaggtt cataaagttg
     2701 aacgccgcga tgaggatgat caggataccg atgcttccga agatcatcag gtatttcggg
     2761 tcgttggcac gcttaaaggc ctgctcgatc gacgggtcca ggtgtacttt cgccaggggc
     2821 tgcaggtaca tgctgtactt attgccctgt gcaaagaagt cctccaccgt tacgccaagg
     2881 aacgactgca ggatcggtcc cacatacttg acgatcatat cggggatcct ggcctctgcc
     2941 gattgggggg aggcaccggg atggagcagg acgtatgtgc tgaagctgtt tgagagccac
     3001 tcgttgtcgc cggccctcgg attggtcata aaggaactga tgatattggc tgcgaaatga
     3061 gacgttccgg ggatatcttc cataatgccc gtcacgcggt atgggaccgt atcggtgctg
     3121 actttcagca tcctgtcgat ggggtcttct tcgccgaata tctttttagc cgtcgattcc
     3181 gacaggacca gcgtgtgcgg ctcattcagg acctcttttg catcccccct gatcagcggg
     3241 accgagaaga aaccgaagaa cgacgagtcg gcttcgatga agtccttttc ggtgaagttg
     3301 cgctcgccat agcggatgat cgtttcgccc cagttattca tgcgcagaaa gtcctccact
     3361 tcagggaatt cgttcttcat cgtcggcccg atcggtgaag ccgtgtaggt caccttcacc
     3421 tcctgcccgc ccagcttgcc attcaggatc acccggtaga tccggtcttt tttctcgttg
     3481 aaccggtcgt agctcagttc gtggatgatg aacagggcga tgatcatact gcagaccata
     3541 ccgacgagga gcccggtgat attgatggcc acatgcgccc gatgacggct cagggagcgg
     3601 aagctgtatt tcaggaggat tctgaacata atgcggttta gtgtaaaaga cgcggaaaag
     3661 gcggaatagt tacagggggc gttggtcgtt ggtcggcagt tggcaaattt ggaaatttgg
     3721 aaatttggaa atttggaaat ttggaaattt ggaaatttgg aaatttgnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa cactggaaca atggaactct ggaacattag
     3901 aacccggaac ctggaacctg gaacactgga acattggaac ctggaacctg gaacccggaa
     3961 cctggaacct ggaacactgg aacaatggaa cactggaaca ctggaacact ggaacggtcg
     4021 cgaaaaaata atttcctacc catatcgatc ggttcgatat tttccattaa ttttatagtc
     4081 aattaattaa ccactcattc attaaccagg atctgaagag tttaccgatg tcattattta
     4141 aatcagcggg cggtatagtg atcaaatcca ttgatgcagg taatgataca tgcatgattc
     4201 agggggataa caggaaaggt ggggatgtat aaacgttagc cccaataatt tttattaaag
     4261 atctttttgg tgaagagtga gccgtgaa
//
LOCUS       DAOQXS010000252         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21107_length_4269_cov_4.574039, whole genome shotgun sequence.
ACCESSION   DAOQXS010000252 DAOQXS010000000
VERSION     DAOQXS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21107_length_4269_cov_4.574039"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKM34_07680"
     CDS             complement(<1..498)
                     /locus_tag="PKM34_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ83507.1"
                     /translation="MQFSEQEQIRRNSLQELINLGIDPYPAEGFNVSRNIHDIVAEYE
                     PVKSKYYEDGKLKDPVVEPEYEKIEVSLAGRIMNRRIMGAASFAEIQDSTGRMQIYIK
                     RDEICPGDDKTMYNTVFKRLLDIGDIIGVKGFVFITHMGEITVHVKEYKLLSKSLRPL
                     PIVKEK"
     gene            complement(659..1408)
                     /locus_tag="PKM34_07685"
     CDS             complement(659..1408)
                     /locus_tag="PKM34_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83508.1"
                     /translation="MVSAAQPGIPPRCPYKAIQFPDSRVVKEAQDFTIITTDGVTRNL
                     YNTLDSGKTVFIDIFYTTCYYCQVYAPVIEEIYQNTGAGEGDIEFWGISNNLFDTNNV
                     IDEYREQYNITNPCAGPWGDGEIAFTILVNGQNFNGFPTYCVICPDRTLYFDACYPPT
                     TTCFDPFFEQCASIGINDDIIADLQSKHKGPVTVEVYDLVGRKIFSGNFESEPDLKSL
                     SSSVQDNGMYVIRILNDNRLIDIHKIVITGR"
     gene            1734..2588
                     /gene="lipA"
                     /locus_tag="PKM34_07690"
     CDS             1734..2588
                     /gene="lipA"
                     /locus_tag="PKM34_07690"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNQ83509.1"
                     /translation="MEENRGRKKPDWLKIKVPKGKEYIGVRDIIDRNRLHTICSSGQC
                     PNKEDCWGRGTATFMILGNICTRSCKFCAVPTGRPLPADEAEPARIAESVQKMKLKHC
                     VITSVDRDDLEDGGANIWAATIRAVKELNPATTIETLVPDFRGRKELLQIVIDAWPEV
                     ISHNLETVRRLTPQVRSAAIYERSLEVVGWVAESGIRSKSGIMLGLGETEEEVVQVMK
                     DLRRVGCEVVTIGQDLQPTAAHLPVKEYIRPEQFEKYRQTGIEMGFRHVESGPLVRSS
                     YHAEKHVL"
     gene            2648..3349
                     /gene="lipB"
                     /locus_tag="PKM34_07695"
     CDS             2648..3349
                     /gene="lipB"
                     /locus_tag="PKM34_07695"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNQ83510.1"
                     /translation="MNFELKYEDLGLIPYRKAWDYQEKLFGGLMKKKLAGEKSFPGHL
                     LFCEHPPVYTLGKSGKKENLLLNEQMLERQGIEFYHINRGGDITFHGPGQIVGYPILD
                     LEQVSIGIREYIEGLEEAVILVLSRFGIKSGRLSGATGVWLDPEIPAKARKICAIGVK
                     ASRHITMHGFAFNVNTDLSFYRYINPCGFIDKGVTSLGKEMGAMQNIEEVKGLLKNCL
                     ESTLGISISEYPNLP"
     gene            complement(3353..4081)
                     /gene="recO"
                     /locus_tag="PKM34_07700"
     CDS             complement(3353..4081)
                     /gene="recO"
                     /locus_tag="PKM34_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNQ83511.1"
                     /translation="MFQATRGIVFRQTRYSDTSLVARILTEESGLRSYIVKGAHRPKA
                     RIRASIFQPLTLLDMVVSHKEKADLQHIREVRIAYPFQTIPADIRKTSILLFLNELFY
                     KSIQEEASNQELFRFIYDNLILLDQSDEHPANFHISMALHLSHFLGFFPQGHYSGFHT
                     VFDLAEGTFTESEPLPAENFISGEVCSWFAKFLTVPVERSYVPEVSAVIRSELLEKIL
                     RYYHLHLPLTGVFKSHKVLHEVLR"
     gene            complement(4081..>4269)
                     /locus_tag="PKM34_07705"
     CDS             complement(4081..>4269)
                     /locus_tag="PKM34_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83512.1"
                     /translation="TVKITDIRGSLIYETMAEYNDTQVEWNGLDMNGRRPNTGVFLVF
                     VSNNDGTETMVTKILFVN"
BASE COUNT         1172 a          946 c         1075 g         1076 t
ORIGIN      
        1 tttttctttc acaatgggaa gaggcctgag cgatttggaa agaagcttgt attccttcac
       61 atggacggtg atctctccca tgtgggtgat gaagacaaag cctttcacgc caatgatgtc
      121 gccaatatcc agtaaacgct tgaacactgt attgtacatg gtcttgtcat cgccagggca
      181 aatctcatcc cttttaatat aaatctgcat ccttccggtc gagtcctgta tttcggcaaa
      241 ggaagcggca cccattatgc gcctgttcat aatgcggccg gccaggctca cttcaatttt
      301 ttcatattca ggctcaacga cgggatcttt cagcttgccg tcctcgtaat atttgctttt
      361 caccggttcg tattcagcga cgatgtcatg aatattcctg ctcacgttaa atccttcggc
      421 aggataagga tcaatgccca ggttgatcag ttcctgaagc gaattacggc gtatttgttc
      481 ctgttcggaa aattgcattg ctaaattgct aaattgttaa actgtttaaa tggatctcta
      541 tatcgtttga atcgcttgta ttttttgcag tccgacgact aaagccgtcg gttcaacctg
      601 aagtcagcag tccgacggtg caaagatagg aaactatcgg taaaaacgtt ttggcgactt
      661 atctcccggt aatcacaatc ttatggatat cgatcaaccg gttatcattc agtatcctga
      721 taacatacat tccgttatcc tgaacagaag atgaaagaga tttcagatcg ggctccgatt
      781 cgaaatttcc tgaaaagatt tttctcccaa caagatcata aacttcgaca gtcacagggc
      841 ccttgtgttt tgactgaaga tcagcaataa tatcatcatt aattccgatg ctggcgcatt
      901 gctcaaaaaa cggatcgaag caggtagtgg ttggcggata acaggcatcg aaatacaggg
      961 tccggtcggg acagatgacg caatacgtcg ggaatccgtt gaagttctgc ccgttgacaa
     1021 ggatcgtaaa ggcgatctcc ccgtcgcccc agggccctgc gcacggattt gtaatgttgt
     1081 attgctccct gtattcgtcg atcacattat ttgtatcaaa cagattgttg gagatacccc
     1141 agaactctat atctccttct ccggcaccgg tattctggta gatttcttcg ataaccgggg
     1201 catagacctg gcaataataa caagtggtat aaaaaatatc gatgaaaaca gttttaccgg
     1261 aatccagggt attgtagagg ttacgggtga caccgtcggt ggtgataatg gtgaagtctt
     1321 gggcctcttt taccacgcgg ctgtcgggga actgaatggc tttatagggg cagcggggag
     1381 gaattccggg ctgcgcggca gaaaccattg ataaaaatat ccaagttatg atgatcaaat
     1441 atttcactta tatatatttt tagatgcaaa tgtaaacata atctatatga tgagatttgt
     1501 tattcaccac agagttacac agattttcac aacttgtccc gcaaggcggg agagtaatac
     1561 agagtatttt gatcatattt aattaacaat aggacagttg gacagcacgt cctgagggcg
     1621 aagcccgaag gatcacagtt taacaattga acaattgaac aatcgaacaa ttgaacaatt
     1681 gaacgccgga tcaatgcaac aatgccctta cctttgcaaa gaaattcata aagatggaag
     1741 agaacagagg aagaaaaaaa ccggattggc tgaaaattaa ggtccctaaa gggaaagaat
     1801 atattggcgt tcgcgatatt attgaccgga acaggcttca cacgatctgt tcatcaggtc
     1861 agtgccccaa taaggaagat tgctggggcc gcggaacggc cacatttatg atcctcggga
     1921 atatctgcac gcgctcatgc aagttttgtg cggtacccac ggggcgcccg ctgccggccg
     1981 atgaagcgga gccggcgcgg attgccgaat cggtacaaaa aatgaagctg aaacactgcg
     2041 tcattacatc ggtcgacagg gatgacctgg aagacggagg cgcaaacatc tgggcggcaa
     2101 cgatccgggc cgtaaaagag cttaatcccg ccacgacgat cgagacgctg gtgcccgatt
     2161 tccgtggccg gaaagagctg ttgcagatag tcatcgatgc ctggccggag gtaatctccc
     2221 acaacctgga gacggtaagg aggctgacgc cacaggtacg ttcggcggct atttacgagc
     2281 ggagcctcga ggtggtcggc tgggtggccg aatcgggaat ccggtcgaag tcgggcatta
     2341 tgctggggct aggagaaacg gaggaagagg tggttcaggt catgaaagat ctccggaggg
     2401 tcggttgcga ggtagtgacc atcgggcagg atcttcagcc cactgcggca catttgccgg
     2461 ttaaggagta catccggccg gagcagttcg aaaaataccg gcaaacgggg attgaaatgg
     2521 gattccggca tgttgagagc ggcccgctgg tgcgatcgtc ctaccacgcc gaaaaacatg
     2581 tgttatgagg gatcgattgt tcaattgttc gattgctaaa tgttaaacta actttgaact
     2641 ttgaactttg aactttgaac taaaatatga agatctgggg ctcatccctt accgcaaggc
     2701 ctgggattac caggaaaagc ttttcggcgg gctgatgaaa aagaaactgg ccggtgaaaa
     2761 atcctttccg ggtcacctgt tgttttgtga acatcctccc gtttacacgc ttggaaagag
     2821 cggcaaaaag gaaaaccttc tgttaaatga gcagatgctg gaaagacagg gaattgaatt
     2881 ttatcacatc aaccggggcg gcgatattac gtttcacggc cccggccaga tcgtcggata
     2941 ccctatcctt gacctggaac aggtttcgat cgggatcagg gaatatatcg aaggactgga
     3001 agaggcggtc atcctggttc ttagccggtt tggaataaag tcggggcggc tttccggcgc
     3061 caccggtgtc tggcttgatc cggagatccc tgcaaaagcc agaaagatat gcgccatcgg
     3121 cgtcaaggcc agccggcata tcaccatgca tggatttgct ttcaatgtca ataccgatct
     3181 ttccttttac cgatacatca atccctgtgg ttttatcgac aaaggggtga catctctcgg
     3241 gaaagagatg ggggccatgc agaacataga agaagttaaa ggattgttaa agaattgttt
     3301 agaaagcacc cttgggatat ccatttccga atatcccaat ctcccgtaat aattacctta
     3361 gaacttcatg taatacctta tgcgacttga atacaccggt cagtggcagg tgaagatgat
     3421 aatacctgag gattttttca agcaattcgc tacggattac agcggaaacc tcgggcacat
     3481 aagacctctc gacaggcaca gtcagaaact ttgcaaacca gctgcaaact tccccggaaa
     3541 taaaattctc cgcgggtaat ggttctgact ctgtaaaggt tccttccgca aggtcaaaaa
     3601 cagtatgaaa gcccgagtag tggccctgag gaaagaatcc aagaaaatgt gaaaggtgca
     3661 atgccatcga aatatgaaaa ttggccggat gttcatccga ttggtcaagc agtatcaaat
     3721 tatcataaat aaaccggaac aattcctgat ttgatgcctc ctcctggatc gatttataaa
     3781 acaattcatt caaaaataaa aggatcgatg ttttgcgaat atcggccggg atggtctgga
     3841 aaggatacgc aatgcggacc tcccggatat gttgcaggtc ggctttttcc ttatggctga
     3901 ccaccatgtc gagaagggta agaggttgaa aaatactggc cctgatcctg gctttcggcc
     3961 tgtgggcccc tttgacaatg taggaacgca ggcccgattc ctcggtcagg atccgggcta
     4021 ccaggctggt gtcggagtag cgggtctgcc ggaaaacgat gccgcgcgta gcctggaaca
     4081 ttaattaaca aacaatatct tcgtgaccat tgtttcggtg ccgtcgttat tggagacaaa
     4141 caccaggaaa acgccggtat tgggtcttct tccgttcata tcgagcccgt tccattccac
     4201 ctgtgtatcg ttatattcgg ccatggtttc atagataaga gatccccgga tatcggtgat
     4261 tttaacggt
//
LOCUS       DAOQXS010000253         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21125_length_4267_cov_3.619183, whole genome shotgun sequence.
ACCESSION   DAOQXS010000253 DAOQXS010000000
VERSION     DAOQXS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21125_length_4267_cov_3.619183"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>4267)
                     /gene="dnaE"
                     /locus_tag="PKM34_07710"
     CDS             complement(83..>4267)
                     /gene="dnaE"
                     /locus_tag="PKM34_07710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNQ83513.1"
                     /translation="RLSSTCSICTPLHDTDGSLVEARDFIIKPDNFTIPYNAAKVHGI
                     TTEFAAENGDDLKLVLEQFIDALQQATVIVGHNVDFDMKIIGAEFVRTRFENYFEGKI
                     KVDTDRESLNYCALPGGIGGGFKLPKLGELHQKLFGDGFAEAHNAAADVRATARCFLE
                     LIRLGIITAGKLGVDPEVVRNFIINHPEPVQPEQITLRDLRQTVVSVQQPEETSLPKS
                     PEPGTLQPAITPFCHLHVHSQYSILQATADVKDIIAKAVEMKMPAMALTDHGNLYGAF
                     AFIGEAVKHKIKPIIGCEYYISLERTRKKFTKDNPDVRYHQVLLAKNLTGYQNLSRLS
                     SIGFLEGFYDGVPRIDKPLIEKYREGLIATTGGLKSEINDLILNIGETQAEEAFCYWL
                     RLFGDDFYVQLNRHGLPEEEHVNKVLLQLARKYNVRYFAANNAYYLAKDDAEIHDMLL
                     CLQLNEYQATPVGHGRGYRFGFPNNEFYLKSREEMEELFHDLPEAIATAGEIADKIET
                     YPLKRNPLMPDYEIPSGFDDANAYLRHVTYQGAEKRYGSITPEIAERIDFELETIKRM
                     GYPGYFLIVQEILHQAREMGVSVGPGRGSAAGSVVAYCLRITDVDPIRYNLLFERFLN
                     PDRISLPDIDIDFDEDGREKVLKWVVEKYGAGRVAQVITFGKMAPKGAIRDIARVKQL
                     PLSESDRLAKLVNMRPGTTFTDAYKESPDLQRERKEGEPLVQETLKGAERIEGQIRNT
                     GTHACGIIIGKNELIDLIPLSITKESDMLATQFDGSQIESVGMLKMDFLGLKTLSIIK
                     DTLDNIRKSKGLSLDIEAIPLTDTKTFELYSKGDTNGIFQFESEGMKKHLRDLKPNRF
                     EDLIAMNALYRPGPMDYIPNFINRKHGREKIEYPLPEMEEILKETYGITVYQEQVMLL
                     SQRLAGFTKGRADVLPKGMGKKNKDLIDELKKDFIEGIKSNDLDEEIAAKIWTDWEKF
                     AGYAFNKSHSTCYAVVSYRMAWLKAHYPAEFMAAVLSRHLSEIKKITFFIDECKHLHI
                     PVLGPDVNESELHFVVNKKGGIRFGMAAIKGVGESAVNAIIEERNANGPFRSIFDFVK
                     RINLRAVNKRALEALAKAGAFDGFQGIHRAQYFQQENPEDNIFLEKIIRHAAIYQEKQ
                     QASQHSLFGDDLTLDLQDPKIPDCPQWSKHHQLKYEREVTGFFISGHPLDEYKAEIDS
                     FCNVTFQDLNAGLSRYLNKAVSFAGMINTVEIRTAKTGNQYANFELEDFTDSYRLSLF
                     SDDFMKFKYLLVEDTYVLIFGKVEVNRKNNRPEIRIKNMILLAEAMERFCKSVSVTFE
                     LDRIDSELVRSVVKIVKSNPGECEIKIRIKDQDGFYLDMFPKKFRVSPAGFIHAISEF
                     EQVNYSVNGFHAVIGN"
BASE COUNT          955 a         1042 c         1089 g         1181 t
ORIGIN      
        1 tgatttcaag tgccatatgt aaattattta atgattaagc gttcaaaaat agtaaaaatt
       61 gtgttggagg atttctcacc ggctaatttc ctatcacagc atggaaacca ttcacggagt
      121 aattcacctg ttcaaattcc gaaatggcat ggatgaatcc ggccgggctg actctgaact
      181 ttttggggaa catatccagg taaaaaccgt cctgatcctt aatcctgatc tttatctcac
      241 actccccggg atttgacttt acgattttca ccacgcttct taccagttcg gaatcgatcc
      301 ggtcgagttc aaaggtaaca ctgaccgatt tgcaaaatct ttccatcgct tcagccagca
      361 gtatcatatt tttaatgcga atctcggggc ggttattttt cctgttcacc tccacttttc
      421 cgaatatcag gacatacgta tcttctacca gcaggtattt gaacttcata aaatcatcgg
      481 agaataacga cagccggtaa gaatcggtaa aatcttccag ttcgaaatta gcatactggt
      541 tgcccgtttt ggcggtcctg atctctactg tattgatcat accggcaaac gagaccgctt
      601 tattcaggta gcggctcaat ccggcgttga gatcctggaa agtcacgttg cagaatgaat
      661 cgatctccgc tttgtattca tcgagcggat gaccggagat aaagaaccct gtgacctccc
      721 gctcatattt aagttgatga tgtttagacc actgcggaca atccggaatt ttcggatcct
      781 ggaggtcgag ggtgaggtca tcgccaaaga gggaatgctg ggaagcttgc tgtttctcct
      841 ggtatatagc cgcatgacga ataatctttt ccaggaagat gttatcttcg ggattttcct
      901 gctggaaata ctgggcacgg tggattccct ggaagccgtc gaaggcgccg gctttagcca
      961 gcgcctccag tgcccgctta tttacagccc tgaggttgat ccttttcaca aaatcgaaga
     1021 tactgcggaa cgggccgttt gcgttgcgtt cctcgatgat ggcgttgacg gcggattcac
     1081 ccacgccttt aatggcagcc attccgaacc ggataccgcc ttttttattg accacaaagt
     1141 gcaattcgct ttcattgaca tcggggccga gcaccgggat atgaaggtgc ttgcattcat
     1201 cgataaagaa ggtgatcttt ttgatttcgc taagatgccg gctcaggaca gcagccataa
     1261 actcggccgg gtaatgggct ttcagccagg ccatccggta cgacacgacg gcatagcaag
     1321 ttgaatgtga cttattgaag gcgtatccag caaatttttc ccagtcggtc cagatcttag
     1381 ccgcaatctc ttcatccagg tcattggatt tgatcccctc gataaaatcc ttcttcagtt
     1441 catcgatcag gtccttattt ttctttccca tccctttcgg caggacatcc gcgcggcctt
     1501 tggtgaagcc agccagccgc tgggaaagga gcatgacctg ttcctggtag acggtgatgc
     1561 cgtatgtttc ttttagaatt tcttccattt cgggaagagg gtattctatt ttttcacggc
     1621 cgtgtttccg gttgatgaag ttcgggatat aatccatcgg gccgggccga tagagcgcat
     1681 tcatggcgat caggtcctca aaacggttcg gtttcaggtc gcgcaggtgc tttttcatcc
     1741 cttccgactc gaactggaat atgccgttgg tatcgccttt tgaataaagc tcgaaagttt
     1801 tggtatccgt cagcgggatg gcttcaatat ccagtgacag gccttttgat ttccggatat
     1861 tatcaagggt atccttaatg atcgaaagag ttttcaatcc cagaaaatcc attttcagca
     1921 tcccgacgct ttcgatctgg ctaccgtcga actgggtggc cagcatatcc gattctttgg
     1981 tgatgctaag ggggatgagg tcgatcagtt cattttttcc gataatgatc ccacaggcat
     2041 gggtacccgt attgcgtatc tggccctcaa ttctttctgc gcctttgagg gtttcctgca
     2101 ccagtggttc cccttctttg cgttcccgtt ggaggtcggg cgattctttg taggcgtcgg
     2161 tgaatgttgt tccgggccgc atattgacca gtttggccag gcggtcggat tcgctcaggg
     2221 gaagttgctt aacgcgggcg atatcacgga tcgctccttt gggtgccatt ttaccgaagg
     2281 taatgacctg tgcgacacgt cctgcgccat acttttcaac aacccacttc aatacttttt
     2341 ccctgccgtc ttcgtcgaag tcgatgtcga tatcggggag tgagatacgg tcgggattga
     2401 ggaagcgttc gaaaagcagg ttataacgga tcgggtcgac gtcggttatc cgcaggcagt
     2461 aggcgacgac cgagccggcg gctgaacctc gtcccgggcc taccgatacg cccatttcgc
     2521 gggcctgatg caggatctcc tgaacgatca gaaagtaacc cggatagccc attcgtttga
     2581 ttgtttccag ctcaaaatca atcctttctg caatttcagg tgtaatggag ccgtagcgct
     2641 tttccgctcc ctggtaggtg acatgcctga ggtatgcgtt ggcatcatca aatccggagg
     2701 gaatctcgta atcgggcatt aacggattcc ttttcaacgg ataggtctct atcttatcgg
     2761 caatttcacc ggcagtggca atagcttcag gaaggtcatg gaacaactcc tccatctctt
     2821 cccggctctt cagataaaac tcattgttcg gaaaaccaaa gcggtaccct cgaccgtgac
     2881 ccaccggagt ggcctggtac tcattgagtt gaaggcagag cagcatatca tggatttccg
     2941 catcgtcttt agccaggtag taagcattgt tggctgcaaa atatctgaca ttgtatttcc
     3001 gggcaagctg aagcaatact ttgttgacat gttcttcttc gggcaagccg tggcggttca
     3061 gctgcacata gaagtcatcg ccgaaaagcc ttagccagta gcagaaggct tcttccgcct
     3121 gcgtttcgcc gatattcagt attaaatcat tgatttccga cttgagcccg ccggtggtag
     3181 cgataagtcc ttcacggtat ttttcgatca gcggtttgtc gattcgcggc acgccatcgt
     3241 agaacccttc caggaagcca atgctggata gcctcgacag gttctgatac cctgtaaggt
     3301 tcttcgccag cagaacctgg tgataacgca cgtcaggatt gtctttcgtg aattttttgc
     3361 gggtccgttc caggctgata taatattcgc acccgataat gggtttgatc ttgtgtttaa
     3421 ccgcttcgcc tataaatgca aaagccccgt agaggtttcc atggtcggtc agggccatgg
     3481 cgggcatttt catctcaacg gctttcgcta tgatatcttt cacatcggcc gtcgcctgca
     3541 ggatggaata ctgcgaatgg acgtggagat ggcagaaggg agtaatggcg ggttgcaggg
     3601 tcccgggttc aggtgatttc ggaagtgatg tttcttctgg ttgttgaacg ctgaccacgg
     3661 tttgccgtaa atcgcgcagg gtgatctgtt ccggttgaac cggttcaggg tggttgatga
     3721 tgaaatttct gacgacctcc ggatcgacgc ctaattttcc ggcggtaatg attcccaggc
     3781 gtatgagctc caggaaacat cgggctgtag cccggacgtc ggcggcggca ttatgggctt
     3841 cggcaaatcc atcaccaaaa agcttctgat gcaactcgcc cagtttgggc agtttgaaac
     3901 cgcctccgat cccaccggga agtgcacagt aattaagcga ttcgcgatcg gtatcgacct
     3961 tgatttttcc ctcaaaatag ttctcaaagc gggttcgcac gaattcggcg ccgatgatct
     4021 tcatatcgaa atcgacatta tggccgacta tgaccgtagc ctgttgcagc gcgtcgataa
     4081 attgttccag gacgagtttc aggtcgtcgc cattttcagc ggcaaactcg gtggtaatgc
     4141 cgtgaacctt cgcggcgttg taagggatgg tgaagttatc gggtttgatg ataaaatcgc
     4201 gggcttcgac caggctgccg tcggtgtcgt gcaacggcgt gcagatcgag caggtgctgc
     4261 tcaacct
//
LOCUS       DAOQXS010000254         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21175_length_4259_cov_3.318506, whole genome shotgun sequence.
ACCESSION   DAOQXS010000254 DAOQXS010000000
VERSION     DAOQXS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21175_length_4259_cov_3.318506"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="hflX"
                     /locus_tag="PKM34_07715"
     CDS             complement(<1..1030)
                     /gene="hflX"
                     /locus_tag="PKM34_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ83514.1"
                     /translation="MPKGIIIKPVVERAVLIGLVKNRFDADRINDYLNELAFLVDTAG
                     GVAVKRFIQRIDLPDQRTYIGQGKLNEIKEFVKNEDINIAIFDDELTPSQSRNVEKEL
                     GIKVLDRNSLILDIFARRARTAHARTQVELAQYEYLLPRLTRMWTHLERQQGGIGLRG
                     PGEREIETDRRIVRDRIALLKEKLKEIDKIKTTQRKGRKELVRVALVGYTNVGKSTVM
                     NLLSKSDVFAENKLFATLDTTVRKVVIGNLPFLLTDTVGFIRKLPHSLVESFKSTLDE
                     VREADLLVHIADISHPHFEEQIAVVNQTLSEIGASNKPVIMLFNKIDAYTFVEKDPFD
                     LTPAGKENL"
     gene            complement(1092..2894)
                     /locus_tag="PKM34_07720"
     CDS             complement(1092..2894)
                     /locus_tag="PKM34_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNQ83515.1"
                     /translation="MNKIASLSKDQLKKKLWEANPEIRALLKKSSDVADARENLFEYL
                     NGLERHYFNIHSEKEYKDFHIIEKTNAKECIRVLKNVIRTENEKIADFSALKVLRDLA
                     LNSEPEIPVSESFLAEFIFLFHGVHARSGITDDSFKLPSDNKKASQLRSKKLDEYARR
                     MDDHLRHYRHGLDPAVIHDRKAASKAILEYYQGTEKDWQDYKWQLKHIITTRKTLEKL
                     VMLDEDERLGLEYAERYRIPFQITPYYLSLFNRKGRTKDDRAIRAQVLPSANYCINVD
                     KNRKSGFDMDFMGERSTSPAEAITRRYPQIVILKPVDICPQLCVYCQRNWEVKPMRES
                     KATRNKTAAAIDWIRNNENISEVLVTGGDPLILGDDYIETILQSLSEIGHVERIRIGT
                     RVLVTLPMRIGDSFIRILRKFHRPGKREICIVTHFEHPMEITPEAVSAIGRIRRAGIS
                     IYNQQVFTYYNSSRFASCALRKQLKLAGVDPYYTFNTKGKEETSDFRVPIARISQERK
                     EEARFLPGLARTDEPVFNVPKLGKSHLRAWQDHELIMINSQGQRVYRFFPWESKVTLV
                     DDFIYTDVSIYKYLMRLKEDGEDPEEYKSIWYYF"
     gene            complement(3133..4128)
                     /gene="trpS"
                     /locus_tag="PKM34_07725"
     CDS             complement(3133..4128)
                     /gene="trpS"
                     /locus_tag="PKM34_07725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ83516.1"
                     /translation="METVVSGIRPTGNLHLGNYFGALQNFVKMQNEHHCYFFIADYHS
                     LTTHPTPSDLHGNVKQVLVEYLASGLDPEKATLYIQSDLPETAELYLLLNMNAYVGEL
                     ERCTSFKEKIRKQPENINAGLLTYPTLMAADIIIHRATKVPVGKDQEQHLEMTRTFAR
                     RFNRMYNHEYFPEPIAFNFGDNLVKIPGLDGSGKMGKSEGEGNAIFLADEPGVIRKKV
                     MRAVTDAGPTEKNQAKPEPIGNLFQLMKVVSKPETVAFFDEQYNNCSIRYGDLKKQLA
                     DDMVSFTEPFREKIKELSGNDAYLHKVAMLGRDKARESARKTISDIRKIIGFREF"
BASE COUNT          940 a         1067 c         1093 g         1159 t
ORIGIN      
        1 tcaggttctc tttaccggca ggggtgaggt cgaacgggtc tttctccacg aaggtgtaag
       61 catcgatctt gttgaacagc ataataaccg gcttattgct ggcaccgatc tccgacaggg
      121 tctggttgac tactgcgatc tgctcttcga aatggggatg cgaaatgtcg gcgatatgga
      181 cgagcaggtc tgcttcgcgc acctcatcga gcgtcgattt aaacgattcc accaggctgt
      241 gcggcagttt ccggatgaag cccacagtgt cggtcagcag gaacggcagg ttgccgatca
      301 ccactttccg gacggttgtg tcgagggtgg cgaagagctt gttctcggcg aatacgtccg
      361 atttgctcag caggttcatg acggttgatt tgccaacatt ggtgtaaccg acaagggcga
      421 cgcgcaccag ctctttccgc ccttttcgct gcgtggtctt gatcttatcg atctctttca
      481 gcttttcttt gagaagggca atccggtcgc ggacgatccg gcggtcggtt tcgatttccc
      541 gttcccccgg gccgcgcaac ccgatgccgc cctgctgccg ctccaggtgg gtccacatcc
      601 gtgtgagccg gggaaggagg tattcgtact gcgccagctc cacctgtgtg cgggcgtggg
      661 cagtccgcgc acgtcgggca aagatatcca ggatcaggct attccggtcg agcaccttaa
      721 ttcccagctc tttctccacg ttccgggact gggagggggt gagttcgtcg tcgaaaatag
      781 cgatgttgat atcctcgttt ttaacgaatt ctttgatctc gttgagtttc ccctggccga
      841 tgtaggtccg ctggtcgggc aggtcgattc gctggatgaa ccgtttgacg gccactccgc
      901 ctgcggtatc aaccaggaaa gccagttcgt tcagataatc gttgatccgg tcggcatcaa
      961 agcggttttt aaccagtccg atcaggacgg cccgttcgac gaccggtttt atgataattc
     1021 cttttggcat tcaaacaggg ttggaaaccc atagggttgg aaaccctttt ccgattatcc
     1081 ggcaaagata atcagaaata ataccaaatg gacttgtact cttccgggtc ctctccatct
     1141 tcctttaatc tcataaggta cttataaata gaaacatcgg tatagataaa atcatctacc
     1201 agcgtcactt tggattccca gggaaaaaac ctgtaaaccc gctgaccctg gctgttgatc
     1261 atgatgagtt catggtcctg ccaggccctc aggtgcgatt tgcccagctt cggcacattg
     1321 aaaaccggtt catcggtgcg ggccagcccc ggcaggaaac gggcctcctc tttccgttcc
     1381 tggctgatac gagctatggg aaccctgaaa tcggatgtct cctctttacc tttcgtgttg
     1441 aaggtataat aaggatccac cccggccaac ttaagctgtt ttcgaagcgc acacgatgca
     1501 aaccgggaac tgttataata cgtgaaaacc tgctgattgt atatgctgat acctgcccgg
     1561 cgaattctcc caatggcaga aaccgcttcg ggagtgatct ccatcggatg ctcaaaatga
     1621 gtgacaatgc atatctcacg ttttccgggc cggtgaaatt tcctgagaat ccggataaag
     1681 ctatcgccaa tccgcatcgg cagtgtgacc agaacccgcg ttccgatcct gatcctctct
     1741 acatgaccga tttcagacag cgattgcaga atggtctcaa tgtaatcatc cccgagtatt
     1801 aatgggtcgc cgccggttac cagcacttcc gaaatgtttt cgttgttccg gatccagtcg
     1861 attgcggcag cggttttatt tcgtgtcgcc ttcgattccc tcatgggttt aacctcccag
     1921 ttgcgctggc aataaacgca aagctggggg cagatatcga caggtttgag aatgacgatt
     1981 tgcggataac gccgggtgat ggcttctgcc ggactggttg atcgttcacc cataaaatcc
     2041 atatcgaagc ccgacttcct gtttttgtcg acgttgatgc aataattcgc actgggcagc
     2101 acctgggccc tgatggcgcg gtcatctttt gtcctgcctt tccggttaaa aagcgagaga
     2161 tagtatggag taatttgaaa cggtatgcgg tatctttctg catattcaag cccaagccgc
     2221 tcatcctcat ccagcataac cagcttttca agcgttttcc gcgtcgtgat gatgtgtttg
     2281 agttgccatt tataatcctg ccaatccttc tctgtgccct ggtaatactc aagtatcgcc
     2341 ttgctggccg cttttctgtc atgaatcaca gccggatcaa gaccatggcg gtaatgccgg
     2401 agatgatcat ccattcgccg ggcatattca tccagctttt tcgacctgag ctgcgaagct
     2461 tttttgttgt cggaaggtag tttaaagctg tcatccgtaa ttcctgaccg ggcatgcact
     2521 ccatgaaaaa gaaagatgaa ctctgccaga aaactttcgg aaacaggaat ttccggctca
     2581 ctattcagtg caagatcgcg gaggaccttt agcgctgaga aatctgcaat tttctcattt
     2641 tcagttcgga tcacgttttt taggacccgg atgcattctt ttgcatttgt tttttctatg
     2701 atgtggaaat ctttgtattc cttctccgaa tgaatgttga aataatgcct ttccagtccg
     2761 ttcagatatt caaagagatt ctccctggca tcggccacat cagatgattt tttcagtagg
     2821 gccctgattt ccgggttggc ctcccataat ttttttttga gttgatcttt cgataatgat
     2881 gctattttat tcattgttaa tgaattttaa atgattgatc cctgtgtgaa ccagctttct
     2941 gctgaaataa aatcaatgat aacggggaaa aagaataaaa ctggtagtca tccgaagaca
     3001 gatgtccaaa gatgctaatt agatgtcaaa taatcaataa gatacaagat ctgttcagca
     3061 ttaacccgtt attgaacggc taaaaataag aaaaaaaatt gaaatagcca aaaaaagggt
     3121 tggaaaccct atttaaaact ccctgaaacc gatgatcttt cggatgtcgg aaatggtttt
     3181 ccgtgcactc tcacgggctt tgtcgcggcc cagcatggcc accttgtgca ggtaggcgtc
     3241 gttgcccgaa agctctttga tcttttcacg gaaaggttcg gtgaacgaca ccatgtcgtc
     3301 cgccagctgc ttcttcaaat cgccgtagcg gatcgagcag ttgttgtact gctcgtcgaa
     3361 aaaggcgacg gtttcgggtt tcgatacgac tttcatcagc tggaacaggt tcccgatcgg
     3421 ctctggcttt gcctggttct tttcggtcgg gccggcgtcg gtcacggctc gcatcacctt
     3481 tttccggatc acgccgggtt cgtcggccag gaagatggcg ttgccttctc cttccgattt
     3541 gcccatcttg ccgctgccgt cgagccccgg tatcttcacc aggttatcgc cgaagttgaa
     3601 ggcgatcggc tcggggaagt actcgtggtt atacatccgg ttgaagcggc gggcgaaggt
     3661 gcgcgtcatt tcgagatgct gctcctggtc tttgcccacc ggtactttcg tggcgcggtg
     3721 aatgatgata tcggccgcca tcagcgtggg ataggtgagg aggccggcgt tgatgttctc
     3781 gggttgtttg cggatctttt ccttgaagga ggtgcacctt tccagttcgc cgacataggc
     3841 attcatattc aggagcaggt agagctcggc cgtttcgggc aggtcgctct ggatgtagag
     3901 ggttgccttc tccgggtcaa ggccgctggc caggtattcc accagcacct gcttcacatt
     3961 gccgtgcagg tcggacgggg tggggtgggt ggttaacgaa tgataatcgg cgataaagaa
     4021 atagcaatga tgttcgttct gcatcttgac gaagttctgc agagcgccga aataatttcc
     4081 gaggtgaagg tttccggtag gccggatgcc actgacaact gtttccatgg cgcaaagata
     4141 gtgaaattag tcttcggtcg gcagttagcg gtcggcagtc gtcagttgac gataaccttc
     4201 cccgtatatc gcctcccttt cctatccgtt accacaacaa catacatccc cagcggata
//
LOCUS       DAOQXS010000255         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21243_length_4249_cov_3.838817, whole genome shotgun sequence.
ACCESSION   DAOQXS010000255 DAOQXS010000000
VERSION     DAOQXS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21243_length_4249_cov_3.838817"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKM34_07730"
     CDS             <1..194
                     /locus_tag="PKM34_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83517.1"
                     /translation="NIRFPCAADRKIVLKTIEGKHVFEKMTSSAFERIDISGYDPGIY
                     ALIIETKGETINAKVVKKD"
     gene            complement(181..2277)
                     /locus_tag="PKM34_07735"
     CDS             complement(181..2277)
                     /locus_tag="PKM34_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNQ83518.1"
                     /translation="MKNRRLLLKRLVPIMYLIILIQVVFSQPTNVDSLRRIVSDLPDN
                     ATKAGQIIQVAKSYLIKQQPDSALFYCNIAYDLSVRIKYLEGEADALYLKSIIYKGNE
                     DLKMALPLAEKCADLFIILNDSHRIAKGYYNLALAYKESENNDLAMVYSQKSLAISRQ
                     IDDSTLVLGNYNCIGSIFSESKTKGDSAAFYYIKALEISKKKGNSAHQAIILNNLGMI
                     FNSEKNYELAKDYIRQSLELSLQLNDLKQIANSKNYLGIIAASEAEYSLAEELYMESL
                     EICKKIDYLRGISDANLNAGDLFYKMKKYREALEYFDNALSGYRQLEFERGIILALMN
                     KSAVYSELGKTSKALEMQDSCLQIMESISDERLKLLIFQNIADNYYKQGNYKNAYETV
                     ISSKALSEKIYDIEKGKAIHSLLVKYEKEQDQSRILNLEKENLRKTSQRNGYLFGGLA
                     IVVVALFTILYLRQRARHERVVSQQKIIQLEEEKKLMAARLLVEGQEEERKRIATELH
                     DGLGVLLSATRMQFSTIMDKSPENKELIERATKMLEQASGDVRKISHNMMPGLLTKLG
                     FYEAVEDLFEKISDTGDIRVMCNITGDQERLPGNREIMLYRIVQELVNNTLKHAGASV
                     MSIRIQILPEALDITYSDDGRGFDYEKALESGSLGLKSIMSRVDFLNGKISVDTQTGK
                     GAAFHIVVPVTILNPS"
     gene            complement(2312..2761)
                     /locus_tag="PKM34_07740"
     CDS             complement(2312..2761)
                     /locus_tag="PKM34_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83519.1"
                     /translation="MGSILHKPEQTLAFRFVENESKLYVVSNATVELEQGETIRLRES
                     SPVVMPGGEFGTVIHFDILTNSTSNNTFTAQLNVHVLVRQNMNYTGSPTTYEQKIADI
                     RAQIEYILNTYSPNKAYTIQADVVETTGQVGGQTTSTIDSVGQIIII"
     gene            complement(2852..3166)
                     /locus_tag="PKM34_07745"
     CDS             complement(2852..3166)
                     /locus_tag="PKM34_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83520.1"
                     /translation="MKSEIKYVKEIRIIKEKDEEGLMAIRARGVASVRRIVAPKLVPK
                     DYTEAPGDGIYELDFTLGCNHEEFMDIDLEVEVVFRIRSLPAWVKGVKINAAENSDIE
                     LV"
     gene            complement(3520..4158)
                     /locus_tag="PKM34_07750"
     CDS             complement(3520..4158)
                     /locus_tag="PKM34_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ83521.1"
                     /translation="MIKILVADDHQLLIDGIKTALANIQDIVVAGEAYNGQEVIDQLD
                     AGLSVDVLLLDINMPEMDGLQCTKLVSKQYPKVKILILTQYDEKRLVKQLVKHGASGY
                     LLKDSGRDILEKAIRAVVRGEKYFCERLAIRLISQELDEEKGNPLFSKFSEREIEILR
                     LIAEEYSTQQIADKLFLSYHTIESHRSRMISKAGVRNSAGLVRWANENGYLL"
BASE COUNT         1147 a          976 c          822 g         1304 t
ORIGIN      
        1 ttaacatccg gtttccctgt gctgccgatc gaaagatcgt ccttaaaacc atcgaaggga
       61 agcatgtatt cgaaaaaatg acctccagtg catttgagag aatcgatata agcggatatg
      121 atccgggaat ctatgcgctg atcattgaaa cgaagggaga aaccatcaat gcgaaggtgg
      181 tcaagaagga ttgaggattg ttaccgggac aacaatgtga aaagcagcac ctttgcctgt
      241 ttgtgtatcg actgatattt tcccgttcag gaaatcgacc ctcgacatga tgctttttag
      301 tcccaaagat cccgattcca gcgccttttc ataatcaaag ccccggccat cgtcagaata
      361 ggtgatatcc agcgcttcgg gaaggatttg gatccggatg ctcataacag aagcgccggc
      421 atgtttgagg gtattgttaa ccagttcctg gacgatccgg taaagcatga tctcccggtt
      481 gccgggcaaa cgttcctgat ctccggtaat attacacatc accctgatat caccggtatc
      541 gctgatcttc tcaaaaaggt cttccacggc ttcatagaaa ccgagttttg ttaacaagcc
      601 cggcatcatg ttgtgcgaga tcttcctgac gtcgccgctg gcctgctcga gcattttcgt
      661 cgccctttcg atcagttcct tgttttcagg gcttttatcc atgattgtcg aaaactgcat
      721 ccgggtggcc gacagcaaca ctcccaaacc gtcgtgcagc tccgtggcaa tgcgtttgcg
      781 ctcttcctcc tgcccctcca ccagtaacct ggccgccatc agctttttct cctcctccag
      841 ttgaatgatt ttctgctgcg acacgactct ttcatgcctt gccctctggc ggaggtacag
      901 gatcgtaaac aaagccacaa ccacaatggc cagtccgcca aaaagatacc cgttgcgctg
      961 gctggttttg cgaaggttct ccttttccag gttcaggatc cgggattgat cctgctcttt
     1021 ttcatatttc accagtaaac tgtggatagc tttccctttc tcaatatcat aaattttttc
     1081 agataacgcc ttgctgctaa taaccgtctc ataggcattt ttgtaattac cttgtttata
     1141 ataattatcg gcgatatttt gaaagatcag cagtttcagc cgttcatcac taatgctttc
     1201 cataatctgc aggcaggaat cctgcatttc aagtgcctta cttgttttcc ctaattctga
     1261 ataaacagcc gatttattca tcaaagccaa tatgattcct ctctcaaatt ccaattgccg
     1321 gtaacctgat aaggcgttgt cgaaatattc gagcgcttca cggtacttct tcattttata
     1381 aaataaatct ccggcattca aattcgcatc ggaaattcca cgcaaataat ctattttttt
     1441 acaaatttca agcgattcca tataaagctc ttctgccagt gaatattccg cttcactggc
     1501 agcaatgatt ccaaggtaat ttttactgtt ggctatttgc ttcaggtcat ttaattgcaa
     1561 gcttaattca aggctttgac ggatataatc ttttgccagt tcataatttt tctcagaatt
     1621 gaagatcatt ccaaggttat tcaatatgat agcctgatgc gcagaattcc ctttcttttt
     1681 gctgatttca agagctttaa tataataaaa agctgctgaa tccccctttg ttttgctttc
     1741 tgaaaaaata ctgccgatac aattataatt tcccaggact agagtagaat cgtcaatttg
     1801 ccggcttatt gccaggcttt tttgtgaata gaccatggcc aggtcattgt tttcagattc
     1861 cttataagca agagccaggt tataataccc tttggcaatg cggtgactgt cgttgagaat
     1921 aataaataga tctgcacatt tctcggcaag cggcaaagcc attttcaggt cctcatttcc
     1981 tttgtaaata atgcttttaa gataaagtgc atccgcttca ccttccaggt actttatcct
     2041 gacggaaaga tcatacgcta tattacaata gaaaagcgca gaatcgggct gttgttttat
     2101 taagtaactc ttggcaacct gtataatttg accggccttg gttgcattat ccggaaggtc
     2161 gctgacaatg cggcgcaagc tgtcaacatt agtaggttgt gagaaaacga cctggatcaa
     2221 aatgattagg tacatgatag gaaccagccg ttttaaaagc aagcgtctgt ttttcatcag
     2281 tcaggagttt tatcaggttc caatgattcc cttaaatgat tatgatctgg cctactgaat
     2341 caatagttga ggtcgtttgc ccgccaactt gcccggtcgt ctccaccaca tccgcctgaa
     2401 tggtataagc cttgtttgga gagtacgtat ttaaaatata ttcaatctga gcccggatat
     2461 ctgcaatttt ctgttcatat gtcgtcggtg aacctgtata gttcatgttt tgcctgacca
     2521 ggacatgaac gtttaattgg gcggtaaagg tgttattgct ggtactgttg gtcaggatat
     2581 caaaatggat aactgttcca aattcgcctc ccggcattac aaccgggctt gattcacgta
     2641 accttattgt ttcgccttgc tcaagctcga ctgtggcatt tgagacaaca tatagtttcg
     2701 actcgttttc aacaaatctg aaagccaggg tctgttcggg tttgtggagg atacttccca
     2761 tatcattgtt gtttaaggtt tagagtccgg tcataaagat gatgaaaaaa tacacaattt
     2821 ttatctctaa agatactatt tgtgaattta atcataccaa ttcaatatct gaattttcgg
     2881 ccgcattaat tttaacgccc tttacccatg ccgggaggct cctgatccgg aaaactactt
     2941 ctacctccag gtcgatatcc ataaattctt catggttaca acctaatgtg aaatcaagtt
     3001 catagattcc atctccgggc gcttcggtat aatctttcgg caccaactta ggagccacaa
     3061 tacgcctgac tgaagcaacc cctcgggccc ggatagccat gagtccttcc tcatcttttt
     3121 ccttaatgat ccggatctct tttacatatt tgatttcact tttcatctta caatgttatc
     3181 cattataaaa tgaagaagat tagctgaaaa cattgaaaaa aaaaaattag ctgtttccag
     3241 ctaatattat tctaaaagca ctgcatacct ttgatctatt aagttcttat aaaaatcctg
     3301 atttggttag aggttaagat cggtggtcaa ccgcaggtcc aggtagtcat aattctgaag
     3361 attatcatag cagaaaatga cctgtaagga agtgtaacat tccttatccc cacccaaaag
     3421 aagcgggaat aattattccg aaatcatccg ccacattccc ggcgaaaatt aatccggatt
     3481 aaccttacca tcaggaaccc cgatccagtc ggggtttttt tataacagat aaccgttctc
     3541 attagcccac cggaccagtc cggcagagtt cctgacgccg gctttgctga tcatccgtga
     3601 ccgatggctt tcaatggtat ggtaactaag aaataattta tcggcaattt gttgtgtgga
     3661 atactcctcc gcaatcagcc ggagaatctc aatctctctt tctgaaaatt tagaaaataa
     3721 cgggttgcct ttctcttcat ccagttcctg gctgatcaat cgtattgcca gacgctcaca
     3781 aaaatatttt tcacccctca caaccgcccg gatggccttc tccagaatat cccggcccga
     3841 atcttttaaa agataacccg aagcgccatg cttgaccaat tgttttacca gccgcttttc
     3901 atcatactgg gtaaggatca ggattttaac tttcggatac tgctttgaaa ccagttttgt
     3961 gcattgtaat ccatccattt ccggcatatt gatatccagc agaagaacgt caactgagag
     4021 ccctgcatct agttgatcaa taacctcttg accgttgtat gcttcaccgg caaccacaat
     4081 gtcctgtata tttgccaggg ccgttttaat cccgtcgata agcagttggt ggtcatcggc
     4141 aaccagtatt ttgatcattt tgacaggatt gtttttaaca aatttaatgc gaattactta
     4201 gtacatgaca aaaattaata acaacttgaa tatcaataat atatgttaa
//
LOCUS       DAOQXS010000256         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21265_length_4246_cov_3.979003, whole genome shotgun sequence.
ACCESSION   DAOQXS010000256 DAOQXS010000000
VERSION     DAOQXS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21265_length_4246_cov_3.979003"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKM34_07755"
     CDS             <1..1057
                     /locus_tag="PKM34_07755"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ83522.1"
                     /translation="SRLLTQLAREYRVATQMGNQGNSDEGIRQLCEWLWDGAIGEIRE
                     AHAWTNRPIWPQGLERPLETEAIPPTLDWDLFLGPAPLRPYHSAYHPWNWRAWWDFGT
                     GALGDMGCHIFDPVFKALRLRNPQYLYGSSTQVNTESAPKASVVHYEFPDRGEYKNLK
                     LVPVHVTWYDGGLLPPRPAELKDGEPFGDWSGGCLFIGTKGKLLCDSYGRNPRLLPEQ
                     LDKDYVRPVPYLRRIEKAMDGGHELDWIRACKESPDSRTECSSNFDYAGPMNEVVVMG
                     NLAVRLQDLKRKLQWDADKMIIPNISDDDEIRVVTTDKFTVINGHPHFDTQYATLKAK
                     PAAEEYIRHTYREGWKL"
     gene            1244..1960
                     /locus_tag="PKM34_07760"
     CDS             1244..1960
                     /locus_tag="PKM34_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNQ83523.1"
                     /translation="MMIIILSMITSGAMSQGKENMLTKKEKKEGWALLFDGKSANGWR
                     GYNKTAFPEKGWAVEEGTLHVIGSSRGEAGGGGDLLFDKKFRNFELSIEWKVSEGGNS
                     GIFYLAQEIPGEPVWKSAPEMQILDNEKHPDAKLGVDGNRAAGSLYDLIPGDFKAVKP
                     AGQWNQVRIMVYKGTVVHYVNGKAVLEYHLWTEDWKNMVLNSKFKDYEWFLNTAEEGY
                     IVLQDHGDDVWFRNIKIREM"
     gene            1975..3492
                     /gene="amrB"
                     /locus_tag="PKM34_07765"
     CDS             1975..3492
                     /gene="amrB"
                     /locus_tag="PKM34_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ83524.1"
                     /translation="MKCRLRLTGMILLPLFFGSGSHAQDGGSKSVTVDRKPAVAGQFY
                     PSDPVRLRTGLSALFAAAQPCETKNTVAIISPHAGYVFSGEVAASAFNQIDPDKNYDN
                     IFILASSHRMSFDGASVYTRGDYITPLGAVKVNRELASSLVKENPVFTDVPDAHLYEH
                     SLEVQLPFLQYRMKTEFRIIPIVLGTQSAASANKIAQALKPYLNDRNLFIISTDFSHY
                     PEYDDACLVDKATAGAIIKNSPEHLLKLLEENDRKGIDDLATSLCGWTSVVTLLYMTE
                     ERSDLIYREIKYMNSGDSPLYGNKDRVVGYFAIAVEQSSLSKQNDSFSLNEEERKLLL
                     QIARQTMEQYVSKGKVPPLDEKDLPARLRELSGAFVTLKKEGQLRGCIGRFEATSPLY
                     DVVQQMAVASSTQDSRFEPVETDEFDDIDIEISVLSPMRKISSVDEIVLGKHGIYMKK
                     GFYSGTFLPQVATETGWTLKEFLGHCARDKARIGWDGWKTAELFVYEAYVFGEKE"
     gene            complement(3494..>4246)
                     /locus_tag="PKM34_07770"
     CDS             complement(3494..>4246)
                     /locus_tag="PKM34_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ83525.1"
                     /translation="GTVIHQVLFQIFEPFTGKAVDAQSLTASLGAIETLTHKAFRKHY
                     PEGDTELGKNHLILRVVLLFLRNFIKSEIEQLAATDEKKSRLEILSLEKKLEYILTCH
                     PEGKTYDVKIKGHVDRIDRINGMTRIIDYKTGSVQARELSLKDWDQLSTDPKTGKALQ
                     LLIYGWLYLKNKTSDSTDLRVGNISLRRISAGFIPVKVPNEQGFQDDTSDQIEKQLIK
                     LLEEILDPGVPFRQTADAANCKYCPFTSICTR"
BASE COUNT         1077 a         1033 c         1126 g         1010 t
ORIGIN      
        1 atcacgtctt ctgacacaac tggcccggga ataccgggtg gccacccaga tgggcaacca
       61 ggggaactct gatgaaggca tccgccagct ttgcgaatgg ctgtgggatg gcgccattgg
      121 cgaaatccgt gaagcccatg cctggaccaa ccgcccgatc tggccgcagg gcctcgaacg
      181 tcctctggaa acagaagcca ttccgcccac actcgactgg gatcttttcc tcggtccggc
      241 cccgctgcgt ccttaccact cagcgtacca tccctggaac tggcgtgcct ggtgggactt
      301 cggcaccggc gcactgggcg atatgggctg ccatatcttc gacccggtct tcaaagcgct
      361 gcggctccgt aatccgcaat acctctacgg aagctccacg caggtcaata ccgaaagcgc
      421 acccaaagcc tcggtcgtac attacgaatt ccccgaccgg ggagaatata aaaacctgaa
      481 actggtgccg gtccatgtta cctggtacga cgggggcctc ctcccgccaa ggccggctga
      541 gctcaaagac ggcgagcctt tcggcgactg gagcggcggt tgcctcttta tcgggaccaa
      601 aggaaaactg ctctgcgatt cttacggccg caatccgagg ttactgccgg aacagctcga
      661 taaagactat gtccgccccg tgccatacct gcgaaggatc gaaaaagcga tggatggcgg
      721 gcatgaactc gactggatca gggcctgcaa agagagccca gacagccgga ccgaatgcag
      781 ctccaacttt gattacgcag gaccgatgaa cgaagtggtg gtgatgggta acctggctgt
      841 ccgcctgcag gacctgaaac gtaaactgca gtgggatgcc gataaaatga ttatcccgaa
      901 tatttccgat gatgatgaaa tcagggtggt aaccaccgat aaattcactg tcattaacgg
      961 gcatccgcat ttcgacaccc agtatgccac gctcaaagcc aaaccggcag cagaggaata
     1021 tatccggcat acgtacaggg aagggtggaa gctgtgagat ttggcgattt gacgatttgg
     1081 cgatttgatg atttgatgat ttggtgattt ggtgatttgg cgatttgatg atttggtgat
     1141 ttcgtgattt ggtgattttt agctttaaat agtctgtgaa gtctgtgaat gaatcagtgt
     1201 aaatctgtgg tgaataaaat aataatagaa tatgaaaaca acaatgatga ttataatctt
     1261 atcgatgatt acctctggcg cgatgagcca gggaaaggaa aatatgctga ctaaaaaaga
     1321 aaaaaaagag ggctgggcgc ttctgttcga cgggaaatcg gctaacggat ggcgtggata
     1381 caacaaaacg gctttccctg aaaaaggatg ggctgtcgaa gaaggaacgc tgcatgtgat
     1441 cggctccagc aggggagaag cgggcggagg cggcgacctg ctgtttgata aaaaattccg
     1501 caacttcgaa ctgtcgatcg aatggaaagt gtcggaaggt ggcaacagcg gcatcttcta
     1561 cctggcccag gagattcccg gagaaccggt ctggaaaagc gccccggaga tgcagatcct
     1621 cgacaatgaa aagcacccgg atgcaaagct cggagtcgat ggaaaccggg ctgccggctc
     1681 tctgtacgac ctgatccccg gtgattttaa agccgtgaag cctgcaggac agtggaacca
     1741 ggtcaggatc atggtctata aagggaccgt tgtccattat gtcaacggca aagcggttct
     1801 ggagtatcac ctctggaccg aggactggaa aaatatggta ttgaacagca aattcaagga
     1861 ttatgaatgg ttcctgaata cggccgaaga aggttatatt gtgttacagg atcatggaga
     1921 cgacgtctgg ttcaggaata ttaagatcag ggagatgtaa tatagatatt tccaatgaag
     1981 tgtagactaa gattgaccgg tatgatccta ttgcctttat tctttggttc cggcagccat
     2041 gcccaggatg ggggcagcaa gtccgtaacg gttgaccgga aaccggctgt ggccgggcag
     2101 ttctatcctt ccgaccctgt ccggttgcgc accgggctct ccgctttgtt tgccgctgcg
     2161 cagccgtgcg aaaccaaaaa tacggtggct atcatttcgc ctcatgccgg atatgttttt
     2221 tcgggcgagg tggctgcctc cgcctttaac cagatcgacc cggataaaaa ttacgataac
     2281 atcttcatcc tggcatccag ccaccggatg agcttcgacg gcgcttcggt ttatacccga
     2341 ggagactata tcacaccttt gggtgccgta aaggtcaacc gcgaactggc ttcatcactg
     2401 gtgaaagaaa atccggtttt tacggatgtt cccgatgccc acctctatga gcacagcctg
     2461 gaggtccagc tcccttttct ccagtaccgg atgaagaccg agttccggat catccccata
     2521 gtgctgggga cacaatcggc tgcatcggca aacaaaatag cgcaggcatt gaaaccttac
     2581 ctgaatgacc ggaacctctt catcatcagt accgattttt cacactatcc ggaatatgat
     2641 gacgcttgcc tggtcgataa agccactgcc ggtgccatta ttaaaaattc accggaacac
     2701 ctgcttaaat tacttgaaga aaacgaccgg aaagggattg atgacctggc tacaagtctc
     2761 tgcggctgga cttccgtggt gacattgctt tacatgacag aagaaaggag tgacctgatt
     2821 tacagagaaa taaagtatat gaactccggc gattcgccgc tgtatggcaa taaagacagg
     2881 gtagtgggtt attttgctat tgccgtagaa caaagttctc tgtcaaagca gaacgactcc
     2941 ttcagcctga acgaggaaga aaggaagttg ctgcttcaga ttgcacggca aacgatggag
     3001 caatatgttt cgaaaggcaa ggtgcctccg ctcgatgaaa aagacttgcc ggccaggctc
     3061 cgggaacttt caggcgcgtt tgttactttg aaaaaagaag gtcagctgcg gggttgcatt
     3121 ggccggtttg aagcgaccag cccgttgtac gacgtggtgc agcaaatggc cgttgcatct
     3181 tctacacagg attcgcgttt cgagcccgtt gaaaccgatg aattcgatga tatcgatatt
     3241 gagatatccg tcctttcgcc catgcggaag atcagctcgg ttgatgaaat agtactggga
     3301 aaacatggca tctatatgaa aaaggggttc tattccggca ccttcctgcc ccaggtggct
     3361 acggagacgg gatggaccct caaggaattc cttggccatt gcgcccgcga taaagcccgg
     3421 atcgggtggg acggttggaa aacagccgaa ttgtttgttt atgaagcata cgtgttcggg
     3481 gaaaaggaat agcctaccgg gtgcagatcg atgtaaaagg acaatatttg caattggcag
     3541 catcggccgt ctgacggaac ggtacccccg gatcaagtat ctcctccagc aacttaatta
     3601 gttgtttttc aatctgatct gatgtatcat cctggaatcc ctgctcattg ggaaccttaa
     3661 ccggtataaa cccggcgctg atccggcgca gcgaaatatt tccaaccctc agatcagtgg
     3721 aatccgatgt tttgtttttc aggtaaagcc atccataaat cagcaactgg agcgcttttc
     3781 ccgttttcgg atcggtcgag agctgatccc agtctttgag actgagctcc cgggcctgta
     3841 ccgaaccggt tttgtaatct atgatccttg tcatcccgtt tattctgtcg atcctgtcga
     3901 catggccttt gattttcaca tcgtatgtct tgccttccgg atggcacgtc agaatgtatt
     3961 ccagtttttt ttcaagtgaa agtatctcca gcctcgactt tttttcatcc gtggcggcta
     4021 attgttcaat ttcgctttta atgaaattcc ggaggaacaa caaaaccaca cgcaggatca
     4081 ggtggttctt cccaagctcc gtatcccctt ccggataatg cttccggaac gccttatggg
     4141 tgagggtttc aatagcgccc aggctggcag tcaggctctg agcatcaacc gctttgcctg
     4201 taaatggttc gaatatctga aataatacct ggtggatgac cgttcc
//
LOCUS       DAOQXS010000257         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21310_length_4239_cov_3.503585, whole genome shotgun sequence.
ACCESSION   DAOQXS010000257 DAOQXS010000000
VERSION     DAOQXS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21310_length_4239_cov_3.503585"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..2082
                     /locus_tag="PKM34_07775"
     CDS             139..2082
                     /locus_tag="PKM34_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83526.1"
                     /translation="MRVVNVLLCSALIVFSPSVLGQGPLSLDSTCRIIETSMVITPTT
                     CNGASGSITGLTFLGSPPFTFQWLNGAGDPYGSSIDAFGLPAGQYRLTAADGNGCQKN
                     SPYYSIVDAGNLQVTGVQITNPHCDRPDGQIIVQAFSPSGSTLEYSIDDGVSYQADGV
                     FSGLAGGVYVVRVKDVNGCYGFYPFNPIVLADIPGPQVQQIVVTDETDFLSNGSMEII
                     ATGSTATLYYSIDNGNTYQSNNGTFNNLTAGIYICIVKDENDCDTTFMIEIQNIILTY
                     IHIVTGEGGHCLGSTAMVPLDVNNFQSVASFHLRLSYNADKLQCEGFANVHPQLADSL
                     SGLVDPVAGDIHLAWNSSTPVTFIQPETLAELVFTTINPGQGDIQWYTGDTDSYFLDS
                     EGNPVPADFQNGEVMIYEPPEIMLDQSKTVCAGQFVSIMSIATGNHPPFSYRWIYPTG
                     DSTSSDPFFVSVTPADAGLYTLLATDQVGCTDQEIIELIVEEIPIAAFHGNDTLELHT
                     GDMLDAGAGLYSCLWNTGDTTAGIVINAAGMYIVEMESLWGCFGVDSVYVKLTYEEIT
                     ESNVPAILLYPNPAKDILTIQFEEMKDNAGVIIYDIRGKMMVSKRIQQEITTIDAGNL
                     DPGAYFVEISNNHFSIVRKLMVH"
     gene            complement(2246..2317)
                     /locus_tag="PKM34_07780"
     tRNA            complement(2246..2317)
                     /locus_tag="PKM34_07780"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2283..2285),aa:Met,seq:cat)
     gene            complement(2575..3522)
                     /locus_tag="PKM34_07785"
     CDS             complement(2575..3522)
                     /locus_tag="PKM34_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNQ83527.1"
                     /translation="MKKPSVIFLFILLAGSLFSQQEPQYNQHMFTRAIINPAAAGISQ
                     AVDATLFGRHQWIGYKDNDSSPVYPKLYGISLDIPIYAIHSGVGLNLRHYTAGAEKSL
                     DIRLQYAYHFTLNKRNTLSFGMSFNLLNKTIDYSQLLPAEFDPLLESSKAESGLVTDI
                     GLGIHYRYGDKFNAGISSASLLGNSAGIGGPDFKLERHYYGYGSYDIEIQWSGEALVL
                     TPGFLVKTARGAFNADIYALVTYNDFIWGGLMYRVESAAAILAGISYNGIKAGVSYDY
                     TMSKGFAAGSRHSVELFVKYSYPISPKVVKRSGYNTRNL"
     gene            complement(3527..>4239)
                     /locus_tag="PKM34_07790"
     CDS             complement(3527..>4239)
                     /locus_tag="PKM34_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83528.1"
                     /translation="NNVLSQKRAQSVVDFIVSQGIDPGRLVAKGYGKRIFRMLDKDIV
                     KDGYTFKAGTELNDDFVNRLPSKEAREAAYQLNRRTEFAVIAKDYKPGSSAGSTAVIE
                     VISDTSLAALDYNLTPEGKMAVEVYINDYSAGALIDRTINSSLIDEKIVLDLLQKGAI
                     DRNDFQGVFEQILIDDRITENTVILLRKIRLGGIMVGDHPVKVISDAGNKLIIGNDLL
                     EKAGSFIIDESNKKLIFK"
BASE COUNT         1050 a         1092 c          989 g         1108 t
ORIGIN      
        1 gttagcacca ttagtgaaaa ggtggaaact tctaccgcac taattgtcac aagtcgggac
       61 gccatccctt gggatggcgt cccaatttgt tgtatattta cctgttcata tggtattaaa
      121 cccccaaacc agaagaaaat gcgtgttgtg aatgtgcttc tttgttcagc cctgattgtt
      181 ttttcccctt ctgttcttgg tcaggggccc ctttcactcg attctacttg ccgcatcatc
      241 gaaacttcaa tggtaatcac ccctacaacc tgcaatggag ccagcggttc gattaccggg
      301 cttaccttcc tcggctctcc gcctttcacc ttccagtggc ttaacggggc cggtgatcct
      361 tatggctcat ccattgatgc gtttggcctc ccagccggac aataccggct caccgccgcc
      421 gacggcaacg gctgccagaa gaattctcct tattatagca tcgtagatgc agggaatctc
      481 caggtaaccg gggtccaaat aaccaatccc cattgtgacc ggccggatgg ccagattatc
      541 gtccaagcgt tctcgccctc cgggtccact cttgagtatt caatagatga cggcgtttcg
      601 taccaggccg acggcgtttt ctccgggttg gccggtgggg tttacgtggt gcgggttaag
      661 gatgtgaatg gatgttatgg tttttatcct ttcaacccga ttgttctggc agatattccc
      721 ggcccgcagg tacagcaaat cgtcgtcacg gatgaaaccg attttttgtc aaacggatcg
      781 atggaaatta tagcaaccgg aagcacagca acgctctatt actctattga caacggcaac
      841 acctaccaat cgaacaatgg aaccttcaac aatttaacag caggtatata tatctgcatc
      901 gtcaaagacg aaaacgactg cgacacgact ttcatgattg agattcaaaa catcatcctg
      961 acttacatac atatagttac cggcgaagga ggacattgcc ttggaagtac ggctatggtt
     1021 cctcttgatg tcaataattt ccaatccgtt gcgtcctttc atttgagatt aagttacaac
     1081 gccgacaaac tgcaatgcga aggcttcgcc aatgttcatc cgcaacttgc cgacagcctc
     1141 tcaggattgg tcgacccggt ggcgggagat attcatttag cctggaattc atctacaccc
     1201 gtaacattca tccaaccgga aaccctggca gagcttgtgt tcaccaccat taatccagga
     1261 cagggcgaca ttcaatggta cacgggtgat accgatagct atttcttaga ttccgaaggc
     1321 aatcctgttc cggctgattt ccagaatggc gaggtgatga tttatgaacc accggagatc
     1381 atgctggatc agagtaaaac tgtctgcgcc gggcagttcg tcagcataat gagtattgcg
     1441 acaggcaacc acccaccctt tagttaccgt tggatttatc cgacgggcga tagcaccagc
     1501 tccgacccgt tcttcgtcag cgtcaccccc gctgatgccg gcctctatac tctgcttgcc
     1561 accgaccaag ttggctgcac cgaccaggaa attatcgagc taatcgtaga agagattccg
     1621 atagctgctt ttcatggcaa tgatacgctg gaattgcaca ccggggatat gctggatgcc
     1681 ggggccggac tttattcctg tctgtggaat accggcgaca ccacggcagg tattgtaatc
     1741 aatgccgcag gaatgtatat tgttgagatg gagtctcttt ggggttgttt cggtgttgat
     1801 tcggtatatg tcaagctgac atatgaagaa atcaccgaaa gcaatgtgcc cgcgatcctc
     1861 ctctatccaa atcccgccaa ggatattctg actattcagt tcgaagaaat gaaagataat
     1921 gccggagtga tcatatacga tatccgggga aagatgatgg tttcaaaacg gattcagcag
     1981 gaaattacca cgattgacgc tggaaacctt gatccgggtg cctatttcgt tgagatatca
     2041 aacaaccatt tcagtatagt ccggaagctg atggtgcatt aaggaatttt atagtcgatc
     2101 ccatcctgcc tttttggaac cgacggcttt tgtcgtcaaa agaagaaccc cagttgaacc
     2161 aatcgggacg ccatcccaag ggatggcgtc ccgattggtt caacaaaaaa cccgacctgt
     2221 tgctataggc cgggcttttg ttttgtagcg ggggctggat tcgaaccaac gaccttcggg
     2281 ttatgagccc gacgagctac cactgctcca ccccgcaata tgtcttcagg gatcttcgga
     2341 atttgagtcc gacgatccgc cagccggcgg atccaccccg caatataacc gttcaattta
     2401 atccaaacgg ggtgcaaaga tacaataaat atttaataaa ttccaagcac caaattccaa
     2461 attccaaaca aaaatcaaat ccaaaaaaat aatttcaaaa caatttagat tttaaatttg
     2521 atattttgaa tttgcttgga atttggtgct tggaatttgg tgcttggaat tttgctaaag
     2581 gttcctcgta ttgtacccgc tgcgcttgac caccttcggt gaaataggat aggaatattt
     2641 tacaaataat tccacactgt gacggctgcc ggcagcaaaa cccttgctca tcgtgtaatc
     2701 gtacgacaca cccgccttta tcccgttata actaatgcct gccaggatgg cggcagcgct
     2761 ctcgacccgg tacatcagcc cgccccagat gaagtcgtta taagtcacca gggcataaat
     2821 gtcggcattg aatgcccctc tggccgtttt gaccaggaaa cccggcgtga gtactaaagc
     2881 ctctccgctc cactggattt cgatgtcgta actgccgtac ccatagtagt gtcgctcaag
     2941 tttgaaatcg ggaccgccga taccggccga attgcccagc aggctggccg atgaaatgcc
     3001 ggcattgaat ttatcgccgt aacggtaatg gataccgagc ccaatgtcgg tgaccagacc
     3061 gctctccgct ttactgctct ccagcagggg atcaaactcc gcggggagga gctgcgaata
     3121 gtcgatcgtc ttgtttaaca ggttgaacga catcccaaag ctgagggtgt tccttttatt
     3181 gagagtgaaa tgataggcat attgcagccg gatgtcgagg ctcttctccg ccccggctgt
     3241 ataatggcga agattcaacc caacgccgct gtggatggcg taaatgggaa tgtcgagaga
     3301 aataccgtac aacttcgggt agaccgggga actgtcatta tccttgtacc cgatccattg
     3361 atgcctgccg aaaagggtcg catcgaccgc ctgcgaaata ccggcggctg ccgggttaat
     3421 gattgcccgg gtgaacatgt gctggttgta ctgcggctcc tgctgcgaga acagacttcc
     3481 ggccagcaaa atgaaaagaa atatgacgga tggtttcttc atatttctat ttgaatatca
     3541 gttttttatt cgattcgtca ataataaacg aaccggcctt ttccagcagg tcgttgccaa
     3601 tgatgagctt gtttcccgca tcgcttatga cttttacagg atggtctccg accatgatcc
     3661 cgcccagcct tatctttctg agcaaaatga ccgtgttttc cgtaatccgg tcatcaataa
     3721 ggatctgttc gaataccccc tggaaatcat tccggtcgat cgctcctttc tgcaagaggt
     3781 ccagcacgat cttttcatcg atcagtgatg agttgatagt cctgtcgatc agtgccccgg
     3841 cgctgtaatc gttgatgtat acctcaacgg ccatcttgcc ttccggcgtt agattgtaat
     3901 ccagcgccgc cagcgatgtg tcggatatca cttcgataac cgctgtactt cctgctgaag
     3961 aaccgggctt gtaatccttt gcaattacgg caaattcggt ccgccggtta agctgatacg
     4021 ccgcttcacg ggcctctttc gaagggagac ggttgacgaa atcatcattc agctccgtgc
     4081 ctgctttgaa agtataacca tccttaacga tatctttatc cagcatcctg aatatccgct
     4141 taccgtatcc tttcgctacc agccgtcccg gatcaatacc ctggctgaca atgaaatcga
     4201 cgaccgactg cgcccttttc tgcgacagca cattgttgt
//
LOCUS       DAOQXS010000258         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21357_length_4234_cov_2.892558, whole genome shotgun sequence.
ACCESSION   DAOQXS010000258 DAOQXS010000000
VERSION     DAOQXS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21357_length_4234_cov_2.892558"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /locus_tag="PKM34_07795"
     CDS             complement(<1..2103)
                     /locus_tag="PKM34_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83529.1"
                     /translation="MTPFLQRLAREIVRMPVDDPGKVCVVLPNRRAGLYLKKYLAKEL
                     QKAAWAPATYSIEDFITTISGFGMADPAGLLFEFYKVYTDNVREKAQDFEHFTDWAEV
                     LLQDFDEIDRYMADPEKVFHYLDAARALSVWNLNAAPLTQQEQDYVEFYRSLNDLYRE
                     LRSRLTAQKQVYQGLAYRLTAENIEEIRKTLPWNKIIFAGLNALSVAEVQIIDSLVSG
                     SHAELFWDADAYYTDNPLMEAGVFLRQHFQKWPREKQSWIGTDFRDGAKKIKVTGIPG
                     IAGQARLAGDIIMKMKMDAEAPDHTAIVLADEKLLLPVLYTLPEDIGPVNVTMGYPFR
                     LTQLYHLISLFFRMQETAIQLSERRRSTDKAFYVKDILKLLAHPYLQMFAPESGSDAP
                     DFRQIADILKNRNRIFLRPGELLKFSASSGDEFRNLNELLFQFWESPSSGLRCLMALT
                     GLIRDKVIAMDSQPYIDRDLDIEYLYHFSGILNRSIAMAEQYPFIKSVSSLKKILFRL
                     LDNSRLPFTGEPLRGLQVMGVLETRAIDFENIVVLSVNEGILPSGRVPNSFIPFDIKT
                     EFGLPTFRQKDAVFAYHFYRMLQRAENIHLIYDTEGDQMKGGERSRFIMQLGFELKKY
                     NPGISFDESLEKPSSELSGNVKEIIIPKSETILERLSAKTLSGFSPTAFSLYITCPLK
                     FYFQEVLGLAETESVEETI"
     gene            complement(2100..2846)
                     /locus_tag="PKM34_07800"
     CDS             complement(2100..2846)
                     /locus_tag="PKM34_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ83530.1"
                     /translation="MKALILAAGLGTRLYPLTADTPKALVQVNGMTMLERAIRKLASA
                     GFNEIVVNVHHHAEKIKDFLRDLDLPGITTAISDESDLLLDTGGAILKARPFLDDRDP
                     FLVHNVDVISNIDLQALLIDHLECGNMATLSVIDRPTKRYFLFDSEMGLRGWTDTSTG
                     EIRWTGRPAVNVSRLAFSGIHIISPAFFKVLRQSGRFSIIQTYLDMAVQHPIRGHTQE
                     GAVWFDLGKPEQLTMASEYIKNHPELNIYP"
     gene            complement(2843..2986)
                     /locus_tag="PKM34_07805"
     CDS             complement(2843..2986)
                     /locus_tag="PKM34_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83531.1"
                     /translation="MSKKICKEDDRKVIAKKVKKAEFECKTCGAGANKEKYLCKPKRI
                     SGR"
     gene            complement(2991..>4234)
                     /locus_tag="PKM34_07810"
     CDS             complement(2991..>4234)
                     /locus_tag="PKM34_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNQ83532.1"
                     /translation="EHFRRNGLHVPEIYAIDPGREIYLLEDLGDISLFSVVDKSTNQE
                     ITAGIKNLYRKSLSELVRFQVTGHQGLDYSLCYPRQAFDKRAILFDLNYFKYYFLRLH
                     LDFHEDRLEDDFITLTDYLLKTDGNFFMYRDFQARNILIKNNQPYFIDYQGGRKGPLL
                     YDVASLLFQVKADLPFSLRKELLEFYLEELSEMMVIDKTSLRKKYEGFVLIRLLQVLG
                     AYGFRGLIEKKPHFIASIPYALKNLQWWIDQDSFELAIPELRRALAGLAALTQYRLPE
                     ISGSGKQSLMVEIKSFSYKNGYPVDLSKNGGGFVFDCRALPNPGREERYREFTGKDRE
                     IIDYLKDEPAAIAFLDEAEKLVGQSVENYIERGFQNLSVSFGCTGGQHRSVYCAETLA
                     GRLRRRFPAVGFPVTHTNLSD"
BASE COUNT         1023 a         1054 c         1070 g         1087 t
ORIGIN      
        1 gatggtctct tcaaccgatt ccgtttccgc aaggcccagc acttcctgga aatagaattt
       61 aagcgggcag gtgatgtata ggctgaacgc agttggcgag aacccggaaa gcgttttggc
      121 gcttaaccgc tccaggatgg tttccgattt tgggatgatt atttctttca catttccgga
      181 taactccgac gatggttttt ccagggattc gtcgaatgag atcccggggt tgtatttttt
      241 cagttcaaaa ccaagctgca tgatgaaacg gctgcgttcg ccccctttca tctggtcgcc
      301 ttcggtgtca taaataagat ggatgttttc ggcccgttgc agcatccggt aaaaatgata
      361 ggcgaatacc gcatcttttt gcctgaatgt cggaagcccg aattcagttt tgatatcgaa
      421 cgggataaat gaattgggga cccggcccga cggcaatatt ccttcattaa cagacagcac
      481 cacgatgttt tcaaagtcaa tggccctggt ttccagaacc cccatcacct gtagccctct
      541 cagcggttcg ccggtaaagg gaagccggct gttgtcgagc aagcggaaaa ggatcttttt
      601 caggctgctg acggatttga taaaaggata ctgttcggcc atggcgatac tccggttcag
      661 gattccggaa aaatgataga ggtattcaat atcgaggtcg cggtcaatgt aaggctggga
      721 gtccatagcg attaccttgt cgcggatcag gccggtcagt gccatcaagc agcggagccc
      781 agaggatggg gattcccaga attgaaacaa aagttcgttc agattccgga attcgtcgcc
      841 ggagcttgct gaaaatttca acagctcacc cggacggagg aaaatacggt tcctgttttt
      901 gagtatatca gctatttgcc ggaaatccgg ggcatcgctg ccggattcgg gagcgaacat
      961 ttgcaggtac ggatgggcca gaagctttag tatatctttg acgtagaatg ctttatcggt
     1021 tgatctcctc cgttccgaca actgtatggc cgtctcctgc atccggaaga acaggctgat
     1081 cagatgatag agctgggtaa gacgaaaagg gtagcccatg gtcacgttca ccggtccgat
     1141 atcttccgga agggtataaa gaacgggcag tagcagtttt tcatctgcca gtacgatagc
     1201 tgtatggtct ggcgcttccg catccatttt catcttcatg ataatatccc cggccagccg
     1261 tgcctgtcct gctatacccg gaatgcccgt taccttgatt tttttggccc cgtcacggaa
     1321 atccgtaccg atccaggatt gtttttctcg cggccatttc tgaaaatgtt ggcggaggaa
     1381 aacacctgct tccattaacg ggttatccgt gtaataggca tcggcgtccc agaaaagttc
     1441 ggcatgagat ccacttacca ggctgtcgat gatttgcacc tcagccaccg acagggcatt
     1501 cagcccggcg aaaatgattt tattccaggg aagggttttc cggatctctt caatattttc
     1561 ggctgttaac cggtatgcca gaccctggta aacctgtttt tgggctgtca gacgagagcg
     1621 cagttcacgg tacaggtcgt tcagcgaccg gtagaattcg acatagtcct gttcctgctg
     1681 tgtcagcgga gcggcattga ggttccagac cgacaaagcc cgggctgcat ccaggtaatg
     1741 gaacactttc tccgggtcgg ccatataacg gtcgatttcg tcaaaatcct gcaggagtac
     1801 ctccgcccaa tcggtaaagt gttcaaaatc ctgtgctttc tccctcacgt tatcggtgta
     1861 caccttgtag aattcgaata gcaaaccggc cgggtcggcc attccgaaac ccgatatcgt
     1921 ggtgataaaa tcttcaatgg aataagtagc cggggcccag gcggctttct gcaactcttt
     1981 ggccaggtat tttttcaggt agagtccggc acggcggttg ggcaaaacaa cgcacacctt
     2041 accgggatca tctaccggca tccggacgat ctcccgggcc aaccgttgaa gaaaaggggt
     2101 catggatata tattcagttc gggatgattt ttaatgtact ctgaagccat ggtaagttgt
     2161 tccggcttgc ccaggtcgaa ccagaccgca ccttcctgtg tatgtccgcg aatcgggtgc
     2221 tgcacagcca tatcgagata agtttggatg atgctgaatc tcccggattg gcgcaacact
     2281 ttgaaaaaag ccgggctgat aatatggatg ccgctaaacg ccagccgtga aacgttgact
     2341 gccggccggc cagtccaccg gatctcgccg gtcgaagtat cggtccatcc ccgcaatccc
     2401 atttcgctgt cgaacaggaa gtaacgcttt gtcggccggt cgatcaccga aagggtagcc
     2461 atattgccgc attcgagatg gtcaattaac aaggcctgaa gatcaatatt gctgatcaca
     2521 tcaacattat gcaccaggaa aggatcccgg tcgtcgagaa aagggcgcgc tttcagaatg
     2581 gcgccgccgg tatcaagcag caggtccgat tcatcggaga tggctgttgt aatgcccggc
     2641 agatcaaggt cccggaggaa atctttgatc ttttcggcat gatggtgcac attgacgaca
     2701 atctcattga aacctgcaga ggccagcttc cggatggccc gttccagcat tgtcatcccg
     2761 ttcacctgca ccagcgcttt gggggtatcg gccgttagcg gatagagccg ggtgcccagc
     2821 ccggcggcta aaatgagcgc tttcatcggc cggaaattct tttgggtttg cagaggtatt
     2881 tctctttatt tgccccggcc ccgcaggtct tgcattcgaa ttcggctttc ttcacctttt
     2941 tcgcgatcac tttccggtcg tcttccttgc agatcttttt cgacataaaa tcaatcactt
     3001 aaattagtat gtgtgacagg gaaaccgacc gcagggaatc gccggcgcaa tcttccggcc
     3061 aaggtctccg cacagtagac cgaccggtgc tgtccgccgg tgcagccaaa acttaccgaa
     3121 aggttctgaa agccccgttc gatatagttt tcgaccgatt gtccgaccag tttctccgct
     3181 tcatccagga aagcgattgc ggccggttca tccttcaggt aatcaatgat ctcccgatct
     3241 ttccccgtaa actccctgta tctttcctcc ctgcccgggt tgggcaaggc gcggcaatcg
     3301 aacacaaaac caccgccgtt ctttgaaaga tcgacagggt agccgttttt gtacgagaaa
     3361 cttttgattt ccaccatcaa agactgttta ccggaacccg aaatttcggg aagccggtat
     3421 tgtgttaatg ctgccagacc ggccagtgcc cggcgtaatt ccggaatagc taattcaaat
     3481 gaatcctggt caatccacca ctgcaaattt ttaagtgcat aaggaatact ggcaatgaag
     3541 tggggttttt tttcgatcag tccgcggaaa ccataagcgc ccagcacctg taagagcctt
     3601 atcagcacaa atccctcata tttttttctg agactggttt tatcaatcac catcatttca
     3661 gataattctt ccagataaaa ttcaagcaat tcttttctca gtgaaaaagg cagatcggct
     3721 ttcacctgga agaggagcga ggccacgtcg taaagcaatg ggccttttct tcctccctga
     3781 taatcaataa aatagggttg gttattctta atcaggatat tgcgtgcctg gaaatcgcgg
     3841 tacataaaga agttgccatc ggttttcaat aaataatcgg tgagggtaat aaagtcgtct
     3901 tccagccggt cttcatgaaa atcgagatgg agtctcagaa aataatactt gaaatagttg
     3961 aggtcgaata atatagcgcg tttatcgaat gcctggcggg gatagcacag cgaataatcg
     4021 agcccttgat gtccggtcac ctggaaccgg accagttcgc tgagcgattt gcggtaaaga
     4081 ttcttaatcc ctgctgtaat ctcttgattt gtcgatttat cgacaaccga aaacagggaa
     4141 atatcgccca ggtcttccag caggtagatt tcccgcccgg gatcgatggc atagatttca
     4201 gggacatgca gtccgttgcg acggaaatgc tctg
//
LOCUS       DAOQXS010000259         4229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21391_length_4229_cov_3.850982, whole genome shotgun sequence.
ACCESSION   DAOQXS010000259 DAOQXS010000000
VERSION     DAOQXS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21391_length_4229_cov_3.850982"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1353)
                     /gene="rimO"
                     /locus_tag="PKM34_07815"
     CDS             complement(55..1353)
                     /gene="rimO"
                     /locus_tag="PKM34_07815"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNQ83533.1"
                     /translation="MNSKSRARKINIVTLGCSKNLVDSEMLMGQLKAAKVDVAHESGL
                     PADVVVINTCGFINDAKEESIDTILQYAEAKKAGKVRKVYVMGCLSQRYRKELEAEML
                     EVDGFFGVNDLPQILRELGVDYRNELVGERMLTTPSHFAYMKISEGCDKKCSFCAIPL
                     IRGKHISRPLDELVRETALLAAKGVKEIILIAQDLTSYGIDLYGKRNLRILLEKLSEV
                     EGVEWIRLHYAYPSNFPIDILDLIRDNPKICKYIDIPLQHISDPVLRSMKRGVTQSKT
                     LRLLETIRTIIPQAAIRTTLIVGYPNETDADFEALKAFVRQQRFDRLGVFTYSPEENT
                     SSYKIPDNIPEDVKKSRMEEIMEIQQEISLEKNSGKIGNIYKVLIDRKEGENYIGRTE
                     YDSPEVDNEILIRSQQALQIGKFYPVRIDRADFFDLYGSV"
     gene            complement(1390..2349)
                     /gene="ftsY"
                     /locus_tag="PKM34_07820"
     CDS             complement(1390..2349)
                     /gene="ftsY"
                     /locus_tag="PKM34_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNQ83534.1"
                     /translation="MGLFSFFSKEKKETLDKGLDKTRQNVFSRISRAIAGKSRVDDEV
                     LDNLEEILVTSDVGVETTLKIIERIQQRVARDKYVGTEELNRVLKEETASLLNESNGD
                     EGFDFGSANLPVPYVIMVVGVNGVGKTTTIGKLAFQFKKAGKTVVLGAADTFRAAAVD
                     QLKIWGERAGVPVITQGMNADPAAVAFDTVKSAHSQGIEIVIIDTAGRLHNKINLMNE
                     LSKIRRVIQKVIPEAPHEVLLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKG
                     GVAIGISDQFKVPVKYVGLGEKIEDLQIFNRMEFVDSLFGEKA"
     gene            complement(2424..2576)
                     /locus_tag="PKM34_07825"
     CDS             complement(2424..2576)
                     /locus_tag="PKM34_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNQ83535.1"
                     /translation="MAKKVIATLQTQGKEFSKLIRMVRSPKTGAYVFKETMLPNDQVK
                     DFIAKK"
     gene            complement(2585..2773)
                     /gene="rpmG"
                     /locus_tag="PKM34_07830"
     CDS             complement(2585..2773)
                     /gene="rpmG"
                     /locus_tag="PKM34_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNQ83536.1"
                     /translation="MAKKSKDQRIQVILECTEHKNSGQPGTSRYITTKNKKNTPERLE
                     MKKYNPILKKVTVHKEIK"
     gene            2860..3762
                     /gene="xerD"
                     /locus_tag="PKM34_07835"
     CDS             2860..3762
                     /gene="xerD"
                     /locus_tag="PKM34_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNQ83537.1"
                     /translation="MNWNPYIKGFTSFLRLEKSLSGNSVEAYERDVVKLVEYLDINQL
                     ELQPKDITLAHLQDFVKWVNQLGMSARSQARIISGLKTFFKYLLLENTIRVNPAELLE
                     SPRTGRALPDTLNRVEIEQIFDAIDLSTDEGERNRTILEFLYGCGLRVSELTGLKISD
                     IRFNEGFLSVIGKGNKQRLVPVGQKALKQLDIYIHQIRSHIPVQRGFEDIIFLNNRGK
                     GLSRVMIFYIIKDLAEKAGVRKTISPHTFRHSFATHLIEGGADLRAVQEMLGHSSITT
                     TEIYTHLDRDYLRSNILQYHPRAK"
     gene            3827..>4229
                     /locus_tag="PKM34_07840"
     CDS             3827..>4229
                     /locus_tag="PKM34_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83538.1"
                     /translation="MFKHKAVILYFAAFILLITIYSCTPEGEKYLKAGIEKSQQGDYQ
                     GAIAEYDKAVKADTFLVEAYINRANAKHKLKDYRGAIRDYNKAVEMEPDRASGYFNRA
                     IVWNDYGDPRRAVYDYTKALEIEPGNARAYFN"
BASE COUNT         1074 a          994 c          973 g         1188 t
ORIGIN      
        1 ccgggatcat taataacaat cccataatga tttttgtttt catgatgaaa aaatttaaac
       61 cgaaccataa aggtcgaaaa aatctgccct gtctattctg accgggtaaa attttcctat
      121 ttgcaaggcc tgctgcgaac ggatcaggat ttcattatcg acctcggggg agtcgtactc
      181 agtccgtccg atataatttt caccttcttt tctatcgatc aacactttat aaatatttcc
      241 gatcttacct gaattctttt ccagtgaaat ttcctgttga atctccatga tctcttccat
      301 tcgggatttt ttaacatctt caggaatatt atcaggaatc ttgtacgatg atgtattctc
      361 ttccggcgaa taggtaaaaa cgcccaggcg gtcgaatctt tgctgcctta caaatgcttt
      421 cagcgcttcg aaatcagcat cggtttcatt ggggtaaccg acaattagtg tggtacgaat
      481 cgcggcctgc ggaattatag tccggatggt ttcaagcaag cgtaaggttt tgctttgtgt
      541 gacacccctt ttcattgaac gcaaaaccgg gtcgctgata tgctgcaacg ggatgtcgat
      601 gtatttgcat attttgggat tatcccggat caggtcgagg atatcaatgg ggaaatttga
      661 aggataggca tagtgaagcc gtatccactc tacaccctca acttctgaga gtttctccag
      721 caaaatcctc aggtttcttt tgccgtaaag gtctatacca taggaggtca gatcctgggc
      781 aatcagaatg atctctttaa caccttttgc tgccaggaga gcggtttccc tgaccaattc
      841 atccagaggc cgggagatat gttttccgcg aatcaacggg atcgcacaaa atgaacactt
      901 tttatcgcac ccctcggaga ttttcatgta agcaaaatgc gacggcgtgg tgagcatcct
      961 ttcacccacc agctcatttc ggtaatccac gcccaattcc cggagaatct gcggcaggtc
     1021 attcacacca aagaatccgt ctacttcgag catctccgcc tccagttcct tcctgtaacg
     1081 ctgcgacagg caacccataa cgtatacttt ccgcactttg ccggcctttt tagcttcggc
     1141 atactgaaga attgtatcga tcgattcttc ttttgcatca ttgataaatc cgcaggtatt
     1201 gattacgaca acatctgccg gcaaaccgga ttcatgggca acatccactt ttgccgcctt
     1261 tagctggccc atcagcattt ccgaatcgac caggtttttg gaacaaccca gggtgacaat
     1321 attaatttta cgagcccggg actttgaatt caagtctaaa gatttgagtt gagaatgttg
     1381 gatttctgtt tatgcttttt cgccgaagag gctatcgacg aattccatcc ggttgaaaat
     1441 ctggaggtct tcgatcttct cccccagtcc cacatatttg accgggacct tgaactggtc
     1501 ggaaataccg atagcaacgc caccttttgc ggttccgtcg agtttagtaa gtgccagggc
     1561 attgacttcg gtagcggcgg taaattgtct tgcctgttca atagcgttct gacctgtgct
     1621 ggcatccaaa acgagcagga cttcatgtgg cgcttccggg atgactttct ggatgacccg
     1681 cctgattttg gagagctcat tcatgaggtt gattttgttg tgaaggcggc ctgccgtatc
     1741 gataatcacg atttcgatcc cctgagagtg agccgatttc actgtatcga aggccactgc
     1801 tgcgggatct gcgttcattc cctgagtgat gacaggtacc cctgcccttt cgccccagat
     1861 cttgagctgg tcgaccgctg cagcgcggaa agtgtcggct gcgccgagga cgaccgtttt
     1921 tcctgctttc ttgaactgga aagccagctt accgatggtt gtagtttttc cgaccccgtt
     1981 aacaccaacg accataataa cataaggtac gggcaggttt gcggatccga aatcaaagcc
     2041 ttcatccccg ttgctttcat tcagcaggga ggctgtttcc tctttcaaaa cccggttcaa
     2101 ttcctctgtc ccgacatatt tatcccgtgc caccctttgc tgaattcttt cgatgatctt
     2161 cagtgtagtc tccactccca catcggaagt gaccaggatt tcttccaggt tatcgagcac
     2221 ttcatcatcg acgcgggatt tcccggctat ggcacggctg atccgtgaga acacattctg
     2281 gcgggtttta tccaggcctt tgtcaagggt ttccttcttc tcttttgaaa aaaatgaaaa
     2341 tagtcccatt ttgtatccat ttttaaataa aaaaagctct tccggcggga aaagcttttc
     2401 ggaaccggtt ttctataaac ccgttatttc ttcgcaatga aatccttaac ctgatcattc
     2461 gggagcattg tttccttaaa aacataagcc ccggttttgg gcgaccgcac catgcggatc
     2521 agtttggaga attcctttcc ctgggtttgc agggttgcaa taactttctt tgccatagct
     2581 cgacttattt aatttcttta tgaaccgtaa cttttttcag gataggatta tactttttca
     2641 tttccagcct ttccggggtg tttttcttgt ttttcgtggt aatgtacctc gaggttcccg
     2701 gctggccgct gtttttatgc tcggtgcatt ccagtataac ctgaatccgc tgatctttcg
     2761 atttcttcgc cattttaaaa gactcctttt taattttggg ctgcaaagat agaattttat
     2821 ttgaaaataa cctataattt tatcaaaaat tacccgacct tgaactggaa tccatacatc
     2881 aaaggtttta cgtcgtttct caggctggaa aaatcgttat ccggtaattc ggttgaagcc
     2941 tacgaacggg atgtggtcaa actggtagaa tatctggata tcaaccagtt ggagcttcaa
     3001 cccaaggaca tcacactggc tcaccttcag gattttgtca aatgggttaa ccagctggga
     3061 atgagtgcac ggtcgcaggc caggatcatc tcagggctta aaacattctt taagtacctg
     3121 ttactggaaa acacgatccg ggtaaatccc gcggagttgc tggaatcacc caggactggc
     3181 agggcattgc ccgacacatt gaacagagtg gagatagagc agattttcga cgcgatcgat
     3241 ttaagcacgg atgaaggcga aaggaaccgt accatcctgg aattcttgta cggatgcggg
     3301 ctcagggtca gtgaattaac ggggcttaag atatcggata tcaggtttaa tgaaggtttt
     3361 ctatccgtta tcgggaaagg aaataaacag cgactggtac cggtaggaca gaaagcgctc
     3421 aagcaactgg atatttacat tcaccagatt cggagccata tacctgtcca gcgggggttt
     3481 gaggatatca tattcctgaa taacaggggt aaaggcttat ccagggttat gatcttttat
     3541 attattaagg atctggccga aaaggcaggg gtccggaaaa ccatcagccc gcatactttc
     3601 aggcattctt tcgccactca cctgatcgag gggggcgccg atctgcgggc cgtgcaggag
     3661 atgctgggcc actcatcgat cacaacaaca gaaatttaca cacatctcga ccgggattac
     3721 ctgcggagca atattctgca gtatcaccca agggcaaagt agcagggtgg ttctaacctg
     3781 aatcgtgaaa catgaaactg aaactcgaaa ctggaaactc ataaatatgt ttaaacataa
     3841 agccgtaatt ctatatttcg ccgcattcat cctgctgatc accatatatt cctgcacgcc
     3901 ggagggagag aagtatttaa aggccgggat agaaaagtcg caacagggtg attaccaggg
     3961 ggctattgcg gaatacgata aagcggtgaa agccgacaca ttcctggtag aggcctatat
     4021 caaccgggcc aatgccaagc ataagctaaa agattaccgg ggggccatcc gggattataa
     4081 caaagcggta gagatggaac cggacagggc gtcgggctat ttcaaccggg cgattgtgtg
     4141 gaatgattac ggtgatcccc ggagagctgt ttacgattat accaaagcgc ttgagataga
     4201 gccgggaaat gcccgtgcct acttcaaca
//
LOCUS       DAOQXS010000260         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21624_length_4193_cov_4.414693, whole genome shotgun sequence.
ACCESSION   DAOQXS010000260 DAOQXS010000000
VERSION     DAOQXS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21624_length_4193_cov_4.414693"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PKM34_07845"
     CDS             complement(<1..1143)
                     /locus_tag="PKM34_07845"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNQ83539.1"
                     /translation="MNFEIKQSHFYLNNKKIFLNSGEIHYFRIKRELWDIHLDAAVEA
                     GLTTVSTYVPWAWHEPEEGQFDFTGKTTPERDLQGWLQRCKAHGLTCILKPGPFILAE
                     TRGAGLPGWFLQKHGREVKMRNSRGEEVASDGVNLFHPVYLEKVGRWYDRIMPFIRDH
                     EMENGGPVIMMQICNEIGVFSWLAHQGDYGEGVKERFGNYLMRKFGNVGEVNRLWGKN
                     YTGFKDVELPPDGREPYVSKGDRGRDYEWHCFWRTYYGDYLRLLTMMARDRGVTVPFY
                     HNLPGWIYGSGYEFPLNITMYDDLFGDRSEILFGVDHIPEYLSHRNQHDDRIINDITA
                     AMQGDKPLFAAEFQCGSREYHVVTNPREMELFYKASLAHGLNGWNFY"
     gene            complement(1219..3003)
                     /locus_tag="PKM34_07850"
     CDS             complement(1219..3003)
                     /locus_tag="PKM34_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ83540.1"
                     /translation="MEEQVKTAVHKTSPEDRIKFSQLAAYGAGGIIPIALFNIAGILV
                     GLMGNISLGLSAFWLGVILIIPRLWDAVSDPIIGHISDNTRTRFGRRRPFLLIGGILV
                     AVFFVVMWWIPKGETVHGWFPTEAGFQAFQLVYILIALLLFYTSTTIFEIPHGALGME
                     MTSDYHERTRLFSAKSFVGNLFAMSTPWLFALANMEIFRGVGGNEADGMRYVSLMIAA
                     LIIPLSFWWTYKLREPGFVRVVKQEKSPFWKDMKHAAGNRNFLMLTFTIFTLAMGFNF
                     VQLLGSYIPIFYVFGGDKVAGAYLLGINGTIWAVTGVLAVFPLNWISPKLGKRNTLVI
                     AILLMVLAQLSKIVCYNPDYPYLIIIPTMLLSAGMLFFFTLGSSMVGDICDEDDLKTG
                     KRTEGSFYSIFWWFIKMGTALASFVAGLLIVLTMFDETQVTKVDALQGSIRELRKEVQ
                     ANTGKGSQEVSLSDFLQKAQTKSSELMKHLEEKAASDKHNKEHFDALLQNMSGVNQRI
                     AGLDLSSSKESLDNEISGIENDITQLTIQSPFTLLMMRIVEIGLPVLLSLLSIFFVLR
                     YSLTEKRSHEIKELLKKRNEEISMGTND"
     gene            complement(3145..>4193)
                     /locus_tag="PKM34_07855"
     CDS             complement(3145..>4193)
                     /locus_tag="PKM34_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83541.1"
                     /translation="SQNQNILKVVDYIESLIAQRSTDYYRNGSDSLRAHYGLVPESIS
                     HEGYSAKPMHSYWDNFFTIKGLKDAAEIQAILGESENYDRIAALRDTFRKNLYNSIDL
                     AMKYKGIDYIPGCVELGDFDATSTTIALTPCNELKNLPKPQVYNTFDKYYRFFLDRRE
                     GRIDWINYTPYENRLIGSFILLDQPERAHELIDFFLNDQRPQGWHHWAEVVWKDDRYP
                     GYIGDMPHTWCGSDFLNAIRSLFVYENEYDQTLVLGAALYQEWIDSPDGMSVENLPTY
                     YGDISFSIKKEKNKYHFKISGDIDLPDNGMEIRNFNGSKKPKKVTVNGIEIEEFSDKK
                     IPVKVFPAEVLIYY"
BASE COUNT         1023 a         1074 c         1012 g         1084 t
ORIGIN      
        1 gtaaaaattc cagccattaa ggccgtgcgc caggctggct ttgtaaaaca gctccatctc
       61 ccgcggattc gtgaccacat gatattccct tgaaccgcac tggaattcgg cggcaaacag
      121 cggtttatcc ccctgcatgg ccgccgtgat gtcgttgatg atgcggtcgt cgtgctggtt
      181 gcggtgcgaa agatactccg gaatatgatc gacgccgaac aggatctcgc ttctgtcacc
      241 gaacagatcg tcgtacatgg tgatattcag cggaaactca tagccgctgc cgtagatcca
      301 gccgggaagg ttgtggtaga agggaacggt gacgcctctg tcgcgcgcca tcattgtcag
      361 cagccgcaaa taatcgccgt aataggtccg ccagaagcaa tgccattcgt agtcccttcc
      421 gcggtcacct ttcgaaacat agggttcccg accatccggc ggcagctcaa catctttaaa
      481 cccagtataa ttcttacccc ataagcgatt gacctccccc acatttccaa attttctcat
      541 caaataattt ccaaatctct ctttcacccc ctcgccatag tctccctggt gtgccagcca
      601 cgaaaagact ccgatctcat tacatatctg catcatgatc accgggccgc cgttttccat
      661 ttcatgatcg cggataaagg gcatgatacg gtcgtaccag cggccgactt tctccaggta
      721 taccggatga aacaggttta cgccgtcgct ggccacctct tcgccccggc tgttgcgcat
      781 tttcacttcg cggccatgct tttgcaggaa ccatccgggc aggccggcac cccgggtttc
      841 ggccaggata aaggggccgg gcttcaggat gcaggtaagg ccatgcgctt tgcagcgctg
      901 caaccagccc tgcagatcgc gttccggcgt ggtttttccg gtgaaatcga actgcccttc
      961 ttcgggttca tgccaggccc agggaacgta ggtgctgacg gtggtcagtc cggcctcaac
     1021 agcggcgtcg agatgtatgt cccacagttc ccgtttaatc ctgaaatagt gtatctcccc
     1081 tgagttcagg aaaatctttt tattgttgag ataaaagtgg ctttgcttta tttcaaagtt
     1141 catgacagct atttgtattt tctacccaac gacgactata gccgtcggtt tattgttcgc
     1201 agtaaggaac caaccgcttt agtcgttcgt tcccatactt atttcctcat tccttttttt
     1261 taataattct tttatctcat gcgaacgttt ctctgttaaa ctgtaacgga gaacaaagaa
     1321 aatcgacagc aggctgagca gtaccggcaa tccgatttcc acgattctca tcatcaggag
     1381 cgtaaaaggc gattgaatgg tcagttgtgt gatgtcgttc tcgatgccac tgatctcatt
     1441 atcaagtgat tctttggaag atgacagatc caggccggcg atccgctggt tgaccccaga
     1501 catattttga aggagtgcat cgaagtgttc cttattgtgt ttatccgacg ccgctttttc
     1561 ttccaggtgt ttcatcagtt cggaggattt tgtctgtgct ttctgcagaa agtccgacag
     1621 ggaaacttcc tgacttcctt tgccggtatt tgcctggacc tctttccgga gctccctgat
     1681 gcttccctgg agggcatcga cttttgtaac ctgcgtctca tcgaacatcg ttaaaacgat
     1741 aagcaaaccg gctacaaagc tggccagggc agtccccatc ttgataaacc accagaaaat
     1801 cgaataaaaa ctcccctctg tccgtttgcc ggttttcaaa tcatcttcat cacaaatatc
     1861 gcccaccatg gaagacccca gggtaaagaa gaacagcatt ccggcggata agagcatggt
     1921 cgggataatg ataaggtaag gataatctgg gttgtagcag acaatttttg aaagctgcgc
     1981 cagcaccatg agcaggatgg cgatcaccag cgtgtttctt ttacccagtt tcggactgat
     2041 ccagttcagg ggaaacacgg ccaaaacgcc ggtcacagcc cagatggtcc cgttgatccc
     2101 cagcaggtaa gcgccggcca ctttatcacc gccaaagaca tagaagatcg gaatatacga
     2161 tccgagaagc tgtacgaaat taaaacccat cgccagggtg aaaatagtga aggtcagcat
     2221 gagaaaattc cggttgccgg cagcgtgttt catatctttc cagaaaggtg acttttcctg
     2281 ctttaccacc cttacaaagc ccggttcacg gagtttataa gtccaccaga atgagagggg
     2341 gatgatcagt gcagcgatca tcagggaaac atatcgcatg ccatcggctt cattacctcc
     2401 gaccccccgg aagatttcca tattggcgag ggcaaaaagc cagggagtgc tcatggcaaa
     2461 caggttaccg acaaagcttt tggcgctgaa caaccgggtt ctttcatggt aatcggaggt
     2521 catctccatg cccagtgcac cgtgaggtat ctcaaaaatg gtagtggaag tatagaaaag
     2581 gagaagcgcg atcaggatat agacgagctg aaaagcctgg aaaccggcct cagtggggaa
     2641 ccagccgtgt accgtttcac ctttcgggat ccaccacatc accacgaaaa acacggcgac
     2701 gagaatccct ccgatcagca ggaagggacg gcgccgcccg aatcgtgtac gcgtattgtc
     2761 cgagatgtga ccgatgatgg gatcggaaac ggcatcccat aaacggggta tgatcaggat
     2821 caccccgagc cagaaagcgc tcaggcccag actgatgttg cccatcaatc cgaccagtat
     2881 cccggcaatg ttgaacaggg cgatggggat gatacctcct gccccgtagg ccgccaactg
     2941 cgagaattta atcctgtctt cagggctggt cttgtgcaca gccgttttta cctgctcttc
     3001 catatttgat ttttttaacc acaaagtaac acaaaggaac gcgaaggatt tttattggta
     3061 attatttact ttgtgcgcat ttgtgatatc ctttgtgcac ctttgcggtt cactttgttg
     3121 acaatctatg taaaattaac cactttaata gtaaatcaac acctcagcgg ggaatacctt
     3181 aacgggaatc ttcttatcgg aaaattcctc gatctcaatc ccgttcaccg taactttctt
     3241 cggcttcttt gacccattga aattcctgat ctccatgccg ttatccggca aatctatatc
     3301 accggaaatc ttaaaatgat atttattttt ttcttttttg atggaaaaag agatatctcc
     3361 gtagtaagtc ggtaaatttt ccaccgacat gccatcgggt gaatcgatcc attcctggta
     3421 aagggcagcg ccaagaacaa gtgtttgatc atattcgttt tcatagacga aaagggagcg
     3481 aatagcgttg agaaaatcgc tgccgcacca ggtatggggc atgtcgccga tgtacccggg
     3541 ataccggtca tccttccaga ccacttcggc ccagtggtgc catccctgcg gacgctggtc
     3601 gttcaggaag aaatcgatca gctcgtgggc cctttcaggc tggtcaagga gaataaagga
     3661 gccgatcagc cggttttcat aaggagtgta attgatccag tcgattctgc cttcacgtcg
     3721 gtcgaggaag aaccggtagt atttgtcaaa tgtgttgtat acctgtggtt ttggaagatt
     3781 tttcagctcg ttgcacgggg tcagcgctat ggttgttgat gtcgcatcga aatcgcccaa
     3841 ttccacgcag cccgggatat aatcgatccc tttgtacttc atggccaggt caatagaatt
     3901 gtacaggttt ttccggaaag tgtcgcggag tgcggctatc ctgtcgtaat tttctgattc
     3961 tcccaggatg gcctggatct cagcggcatc tttcaaacct ttgatcgtga aaaaattgtc
     4021 ccagtatgaa tgcatgggtt tagccgaata cccttcatgg ctgatcgatt cggggaccag
     4081 cccgtagtgc gccctgaggc tgtcgctgcc gtttctgtaa tagtcggtcg accgctgggc
     4141 aatcagcgat tcgatgtagt ccacaacctt caggatattt tgattctgcg acc
//
LOCUS       DAOQXS010000261         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21737_length_4176_cov_3.690852, whole genome shotgun sequence.
ACCESSION   DAOQXS010000261 DAOQXS010000000
VERSION     DAOQXS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21737_length_4176_cov_3.690852"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..3407)
                     /locus_tag="PKM34_07860"
     CDS             complement(66..3407)
                     /locus_tag="PKM34_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83542.1"
                     /translation="MKQIYKLLMTAVPVLPAAGLNGKISTSKRLLLMIVIMISSIGVM
                     YSQVTTSGINGKIYGSNYITLPGATVILVHTPTGTQYSTITDDKGIFRIPNMQSGGPY
                     EMKISYVGYKTLQKSDITLALGQTLTFNESLTEAAVGLTEVEVIEKRDALIDGNRTGS
                     STNVNSNQITTLPSINRSITDYTKLSPQANGNSFAGRDGRMNNITIDGANFNNNFGLS
                     SKALPGGDAQPISLDAIEQISINIAPFDIRQSNFTGANVNAVTKSGTNKWSGTAYTYI
                     RGKNFYGKKIEDKEIELTDQNNLNYGLSVGGPIIKNKLFVFANFERETSDFPGILWNP
                     SDPDNGVNADPDNYISRTTVADLSTMKEYLMTTYGYDAGTYQDFGNFQTKNYKILGKI
                     DWNINKNHRFSIRYNYVKSTNDQQVNATSSPGTRSAFGRIGEMSMSFNNANYGFLNTV
                     GSLSAELNSTWSKSANKFLFTYTQIRDTRSSESDIFPFVDIYKDGNPYMSFGYELFSL
                     DNDVKNNVLTFTDNFNYYLGQHTLTVGASFDKLFFGNSYKRYGTSYYRFASPEDFYNG
                     AAPTTFGITYPYAQAGDGYAELNFGYASVYGQDEWQVLDNLKITGGIRLELPIYFNEL
                     LPNEAITELTFKDLDGNDLILDAGLWPDPKMTVSPRLSFNWDAKNDKSLQVRGGTGIF
                     TGRLPFVWFTNQPTNSGTLQNTVEITNADDLAGLTFNADPFYHLTNTDLFPSAPTKAP
                     GSIAVVDKNFKMPQVWRTDLALDVKLPWNLIGTFEAIYSKAINDVIQYNANQAIADSM
                     FFGADDRERFMNRKINSTVSNAMVLTNTKEGYSYSLTAQLAREFENGFYGFLAYTYSA
                     TKDLTSNPGSAAASAWASNPTYTNQNAPTLSYSQFSVPHRVVGCVSYGIEYAKHLKTT
                     VSLFYQGSSQGRLDYIYSNDMNGDGNSADLMYIPKDESEIIFADITNGDGEVIATAAE
                     QGAAFWQYVDQDKYLSDHKGEYAERYGVVMPWVGRFDVKILQDVFTKIGKTKHTLQFS
                     LDFLNIGNLINSDWGIYRKQTLGNYDITLLKYKSSNSEGVPTYQLNYTKTDSDGKPVF
                     PTSTYSPVLGTTSTWGMQIGIRYIF"
     gene            complement(3600..>4176)
                     /locus_tag="PKM34_07865"
     CDS             complement(3600..>4176)
                     /locus_tag="PKM34_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83543.1"
                     /translation="FEDPSMVQGLDALHLGAEAELSRKIAGWLRMGVSLSFGNWKWKN
                     DVTAFVYNDDMVVTDTINVFADGLYVGDAPQTTVAVDAEIRIMRTVTLNAGWYYFDRY
                     YADFSPNTRTDPDDRSQPFRIPSYHLLDLQALCPFKIGNLKAEASLGCLNVLNNKYIV
                     RGQDGPSHTINDFTGFWGFGRNFFAALKVNF"
BASE COUNT         1092 a          936 c          960 g         1188 t
ORIGIN      
        1 taatttaaaa aaaaagaggc tgctcaaatg attgagacag cctccgggag ttatttggag
       61 attagttaaa agatgtacct gataccaatc tgcattcccc atgtactggt agtacctaat
      121 accggagaat aggtagatgt cgggaaaaca ggcttgccat ccgaatcagt tttcgtataa
      181 ttcagctgat aagtcggaac tccttccgaa ttggatgatt tgtatttcag taaggtgata
      241 tcataattac ccagggtttg tttgcgataa ataccccagt ccgaattaat caggttgccg
      301 atgttcagga agtctaaact gaattgtaag gtgtgttttg ttttaccgat tttagtaaat
      361 acatcctgta aaattttcac atcgaatcta cccacccagg gcatcacaac accatatctt
      421 tcagcatatt cccctttatg gtcagacaag tatttgtcct ggtcgacata ctgccagaat
      481 gccgcacctt gctctgccgc ggttgcaatc acctcgccgt cgccattggt aatgtcggca
      541 aaaatgattt cgctctcatc cttaggaatg tacataagat cggcagagtt accatcaccg
      601 ttcatatcat tggaatagat ataatccagg cgtccctggc tgctgccctg gtagaacaac
      661 gataccgttg ttttcaaatg tttggcgtat tcaatgccat acgatacaca accaaccacg
      721 cggtgaggaa cactgaactg cgaataactc agggtcgggg cattctggtt ggtataggtc
      781 ggattgctgg cccatgccga agcggctgcc gatcccggat tcgaggtcag atccttcgta
      841 gcagaatagg tgtacgccag gaaaccatag aagccatttt caaattctct ggccaattga
      901 gccgttaacg aataggaata cccttctttt gtattggtca gcaccatggc attgctaaca
      961 gttgagttga tcttccggtt catgaaccgt tccctgtcat ctgcaccgaa gaacatggag
     1021 tcggcaatcg cctggttggc attgtactgg atcacatcat tgatagcctt ggaataaatg
     1081 gcttcaaagg ttccgatcag gttccagggt aatttaacgt cgagagcaag atcagtcctc
     1141 cacacctgtg gcattttgaa attcttatcc acaacggcaa tcgagccggg tgccttggtc
     1201 ggagccgacg ggaacagatc ggtattggtc agatgataga acgggtcggc attgaaggtc
     1261 aaaccggcca gatcatcggc attggtgatt tcaaccgtat tctgcagggt tccgctgttg
     1321 gtcggctgat tggtgaacca tacaaagggt aagcggcctg tgaaaatacc tgtaccgcct
     1381 ctgacctgga gcgacttgtc atttttagca tcccagttaa agcttaaacg tggtgaaacg
     1441 gtcatcttcg gatcgggcca caggccggca tccaggatca ggtcatttcc gtcgaggtct
     1501 ttgaaggtca actctgtgat cgcctcattc ggaagcagtt cattgaaata gatcggtaat
     1561 tccagcctaa tgccaccggt aattttcagg ttatccagca cctgccattc atcctgtccg
     1621 taaacgcttg cataaccaaa attcagttca gcatatccgt cgccggcctg tgcatagggg
     1681 taggtaatac caaatgtggt aggagcggct ccattgtaga aatcctcggg cgatgcaaac
     1741 cggtaatagc tggttccgta tcttttgtaa gagtttccga agaataattt gtcgaacgat
     1801 gcacctaccg tcagggtatg ttgtcccagg taatagttga agttatcggt gaaggtcagc
     1861 acattgttct taacatcatt atccagtgag aagagttcat aaccaaacga catataggga
     1921 tttccgtctt tgtaaatatc gacgaacggg aaaatgtcgc tttcgctgct ccgggtatcc
     1981 ctgatctgag tataggtgaa caagaattta ttggccgatt tactccaggt gctgttcaat
     2041 tcagccgaaa gcgaaccgac agtattcagg aaaccataat ttgcattatt aaatgacatc
     2101 gacatttcgc cgatccttcc gaatgcggat cttgtaccgg gtgagctcgt tgcgttaact
     2161 tgctgatcat tggttgattt aacgtaatta taccttatgc tgaaccggtg attcttatta
     2221 atgttccagt caatctttcc taagatcttg tagtttttgg tttgaaaatt tccaaaatcc
     2281 tggtaggtac cggcatcata gccataagta gtcatcaaat attccttcat cgtgctcagg
     2341 tcagcaacgg tagtcctgga gatataatta tccggatctg cattaactcc attatccgga
     2401 tcgctgggat tccataagat gccggggaaa tccgaggtct ctctttcgaa atttgcaaag
     2461 acgaataatt tattcttgat aataggtccg ccgacagata atccataatt caggttattt
     2521 tggtctgtaa gctcaatttc cttgtcttca atctttttgc cgtagaaatt cttgccgcgg
     2581 atataagtat aggcagtccc gctccacttg tttgtaccgc ttttggtgac ggcattaaca
     2641 ttggcgccgg taaaattgga ttgcctgata tcaaacgggg caatatttat gctgatctgc
     2701 tcaatagcat ccagactgat cggctgggca tcaccgcccg gcagtgcttt gctgctcaat
     2761 ccgaagttgt tgttaaagtt ggctccgtcg atggtgatgt tgttcatcct gccgtcgcga
     2821 ccggcaaaac tgttgccatt ggcctgtggg ctcagtttgg tataatcggt gatgctcctg
     2881 ttaatggaag gcagcgtggt aatttgattt gaattgacat tggtcgaaga accggtacgg
     2941 ttaccgtcga tcagtgcgtc tctcttttca atcacttcaa cttctgtcaa tcctactgca
     3001 gcttccgtca agctttcatt aaaagtcaga gtctggccca gcgcgagagt gatatcgctt
     3061 ttctgtaaag tcttataacc gacatatgat atcttcattt catatggacc tccgctctgc
     3121 atgttaggaa ttctgaagat ccctttatcg tcggtaatgg tactgtactg ggttccggtc
     3181 ggtgtatgta ccagaattac agtggctccg ggcaaagtaa tgtaatttga gccataaatt
     3241 tttccgttaa ttccggatgt ggtgacctgt gaatacatca cgccgatgct gctgatcata
     3301 ataacgatca tgagaagcaa tctctttgac gtggaaatct ttccgttcag cccggcagca
     3361 ggtaaaaccg gaaccgccgt cattagtaat ttgtagatct gtttcatatc aacttttatt
     3421 taattttaaa tggtttattt gcggacgaat ttatagaatc aggccggatg acttgttaaa
     3481 attatgttaa caaataaggt tgataaaatg aagataacct gatatccaat tgaaaatcag
     3541 aaatttgttg aagaaatcat tgttaacaac ttgtataatc tgaaaatcaa tggattagat
     3601 cagaaattga cttttaatgc agcgaaaaaa tttcttccga aaccccagaa tccggtaaaa
     3661 tcattgatgg tgtgtgaagg gccatcctgg ccgcgtacaa tgtatttgtt attcaataca
     3721 ttcaggcagc ccaggctggc ttcggctttg agattaccaa ttttgaacgg gcataaagcc
     3781 tgcaggtcga gcagatggta ggagggaatc cggaaaggct ggctgcggtc atccggatcc
     3841 gttcgggtgt tggggctgaa atcggcgtaa taacggtcaa agtaatacca gccagcattc
     3901 aatgtcaccg tgcgcatgat ccggatttcc gcgtcgacgg ccaccgtcgt ctgcggtgca
     3961 tcgcccacgt acaacccgtc ggcaaataca ttgatcgtat ctgttactac catatcatca
     4021 ttataaacaa aggcggtcac atcattcttc cacttccagt tgccgaacga aagagaaaca
     4081 cccattcgca gccagccggc aatcttccgg ctcagttcgg cctcggcacc caggtgcagg
     4141 gcatccaggc cctgcaccat cgatgggtcc tcaaac
//
LOCUS       DAOQXS010000262         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21783_length_4170_cov_3.643256, whole genome shotgun sequence.
ACCESSION   DAOQXS010000262 DAOQXS010000000
VERSION     DAOQXS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21783_length_4170_cov_3.643256"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PKM34_07870"
     CDS             complement(<1..1541)
                     /locus_tag="PKM34_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNQ83544.1"
                     /translation="MKWFKAFRRLKPSVEKMEAFRRLKPSVPGILLLIFIGLSYCGYA
                     QEEAKTLSDGYQVFYYPNGNKSSEGTMRNGKPDGYWKTYNEAGKLVSEGNRKDFELDS
                     AWKFYDENGKKALEINYLKGKKEGIRKTWREDEIIEEMFKNDIKNGLTRYLYPDGKLK
                     KEVNFREGLEEGMSKEFDTDGRVITIITYKSGFITGREIINRYDNTGRKHGPWKYFYD
                     DGTLRMEGSYKHGMENGYFKEYDRNGNLLTTAKFADGVKQENVAELVKLEVRKDYYPD
                     GKIKVAATYNKEGKPEGIRREYAEDGTVEKSYIFRNGIMIGEGVVTEKGERDGFWKEY
                     YDDGKLRAQGKYNKDLKEGPWVFYHPNGQTEQEGSYFKGKPEGEWRWYYAGGQLLREE
                     TCFNGLADGDMKEYDEAGNIITRGKYIEGREDGDWYYKVGDNETEGSYTDGMRNGMWR
                     YRDLSPDGKQGTLRFEGRYIEDNPHGKHTYYWENGMKKEEGEYVNGRKEGDWIIYNPD
                     GTPFIV"
     gene            complement(1538..2503)
                     /gene="nadA"
                     /locus_tag="PKM34_07875"
     CDS             complement(1538..2503)
                     /gene="nadA"
                     /locus_tag="PKM34_07875"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNQ83545.1"
                     /translation="MAEPVSQNTKIDFKKEFIRLRKEKNAILLAHYYQVDEIQDIADY
                     VGDSLGLAQQAAKSDADIIVFAGVHFMAETAKILNPETKVLIPDMEAGCSLADNCPYE
                     PFTEFIKQHPGHIVISYINCSAAVKSVSDIICTSGNAVQIVESFPKDQKIIFAPDKNL
                     GGYINRITGRNMVLWDGTCEVHDILQTEAIIKLKIQNPDAKLIAHPECKAPILEIADF
                     VGSTTALLNFTINDDARKYIVATETGILFQMRKSSPEKEFIIVPSDETCSCNDCPYMK
                     LNTMQKLYLCLKNERPEIVMDPVLMEKARLPIERMLEISKKARLI"
     gene            3099..3548
                     /gene="rpiB"
                     /locus_tag="PKM34_07880"
     CDS             3099..3548
                     /gene="rpiB"
                     /locus_tag="PKM34_07880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNQ83546.1"
                     /translation="MKEKTSIIPIACDHGGYELKEFIKMNLLKDGYEIKDFGTDGPES
                     VDYPDFIHPLAKGVNDGVYPLGIIICGSGNGAQMVANKYPNVRAALCWNEEIARLARL
                     HNNANILSLPGRFVSKEEGWNMVKVFLETSFEGGRHQKRVEKINFKF"
     gene            3580..>4170
                     /locus_tag="PKM34_07885"
     CDS             3580..>4170
                     /locus_tag="PKM34_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNQ83547.1"
                     /translation="MELQKKFLKDAEKVAFDPEHRRKLNHNISKYQVNVEKGKKQFND
                     LELARKKAANTRHKILNDLDKYLIEFESNFEKNGGKVIWASTAKDAVKEIMAIMKNTK
                     AVSVVKSKSMMTEELELNEILEKKKIEAIETDLGEFIVQLAGEKPYHILTPAMHKSKE
                     DVAKLYHEKFGMPLDSTPAAIAAYLRDFKVTVIVGLP"
BASE COUNT         1026 a         1044 c          906 g         1194 t
ORIGIN      
        1 acgatgaacg gcgtcccgtc gggattgtag atgatccaat cgccctcctt ccggccgttc
       61 acatactccc cttcttcctt cttcatcccg ttctcccagt aataggtatg ctttccgtga
      121 ggattatcct caatgtaacg gccctcaaaa cgcaaggttc cctgcttgcc gtcgggcgac
      181 aggtcgcggt agcgccacat gccgttgcgc atcccgtcgg tatagcttcc ttccgtctcg
      241 ttgtcgccga ctttatagta ccagtcgccg tcttcccggc cttcaatata ctttccccgg
      301 gtgatgatgt ttcccgcttc gtcgtactcc ttcatatcgc catccgccag cccgttgaaa
      361 caggtctctt cccgcagaag ctgcccgccg gcgtagtacc accgccactc cccttccggt
      421 ttgcctttga aatagcttcc ctcctgctct gtctgtccgt tcggatggta aaacacccac
      481 ggaccctctt tcaggtcttt gttgtatttt ccctgcgccc tcagcttgcc gtcgtcgtaa
      541 tattccttcc agaaaccgtc gcgctccccc ttctccgtca caacaccctc gccgatcatg
      601 atgccgttgc ggaaaatgta cgacttctcc accgtgccgt cttcggcata ttcccgccgg
      661 atcccctccg gtttgccctc tttgttgtac gttgcggcaa ccttgatctt accgtcgggg
      721 tagtaatctt tccggacctc cagtttcacc agctccgcca cattctcctg cttcaccccg
      781 tcggcaaatt tcgccgtggt cagcaggttg ccgttgcggt catactcctt gaaatagccg
      841 ttctccatgc cgtgcttgta gctcccctcc atccgcaacg tcccgtcgtc gtaaaagtat
      901 ttccacgggc cgtgtttcct gccggtgttg tcgtaacggt tgatgatctc ccggccggtg
      961 atgaaaccgc ttttgtacgt gatgatcgtg atgaccctgc catcggtatc aaactccttc
     1021 gacatcccct cttccagccc ttccctgaaa tttacctcct ttttcagctt cccgtcagga
     1081 tataaatatc tcgtcaggcc gttctttata tcatttttga acatctcttc gatgatctcg
     1141 tcttcccgcc aggtcttccg gataccctcc ttttttccct tcagataatt gatttccagg
     1201 gctttttttc cattttcatc atagaatttc caggcgctgt cgagctcaaa atctttcctg
     1261 ttcccttccg aaaccagctt acccgcttca ttatacgttt tccagtaacc atccggcttc
     1321 ccgttgcgca tcgtcccttc gctcgattta ttcccgttcg gataatagaa aacctggtag
     1381 ccgtcagaaa gggtctttgc ctcttcctgc gcatatccac aatagcttaa accaatgaaa
     1441 atcaataata aaattccggg aaccgacggc tttagtcgtc ggaatgcctc catcttttca
     1501 accgacggct ttagtcgtcg gaatgcctta aaccacttca tatcaatctg gcttttttcg
     1561 aaatttcgag catccgttct atcggtaacc gcgccttttc catcaaaacc ggatccatta
     1621 cgatctccgg acgttcattt ttcaggcaca ggtagagttt ctgcatcgtg ttcagcttca
     1681 tgtaaggaca atcgttgcac gagcaggttt cgtccgacgg aacgatgatg aattcctttt
     1741 ccggcgacga tttcctcatc tggaaaagaa ttcccgtttc cgtggcgaca atatatttcc
     1801 tcgcatcatc gttgatcgta aaattcagca atgctgttgt tgatccaaca aaatcggcaa
     1861 tttcaagaat cggggccttg cattcgggat gggcgatcag tttggcgtcg gggttctgta
     1921 tttttagttt gatgatggct tctgtctgca ggatgtcatg gacttcgcag gtaccgtccc
     1981 ataaaaccat gtttcttccc gtaatgcggt tgatataacc gcccaggttc ttatccggag
     2041 caaaaatgat cttctggtcc ttcggaaatg attcgacgat ctgaacggca tttcccgaag
     2101 tacagatgat atccgatacc gattttaccg cagcgctgca atttatatag gaaattacaa
     2161 tatgtccggg gtgttgtttg atgaattcgg taaatggttc gtaaggacaa ttgtcggcca
     2221 gcgaacagcc tgcttccata tcaggaatca aaacttttgt ctccgggttg agaattttag
     2281 ccgtttccgc cataaagtgc accccggcaa aaacaatgat atccgcatcc gatttggcgg
     2341 cctgttgtgc caggcccagg ctgtcgccca cataatcggc aatatcctgg atttcatcta
     2401 cctggtaata gtgggccagg aggatggcgt tcttttcttt ccgtaaccgg ataaattcct
     2461 ttttaaaatc gatctttgta ttctgactga ctggttccgc catcttttat caattcctta
     2521 ttaatattaa agtttaaaga aatttattct ttttattatg cctgttgata atgatgaaaa
     2581 cttatccttc aaaaatttga caaaccgagt tattcaattt tgttaacagg tattttctgc
     2641 aaatttcttt attattattt gattttcgga aaattaagaa ttattaacaa cctgtcgata
     2701 acccggggtg ttaaaaaatt cagggttatg aacaggttga ggatgtttat aaagagggtg
     2761 aattttcttc atatctcatc ataaaaaccg cataaaatcg attttaacgc gaatttttta
     2821 tttgttaaca aaaccggtgc catttataca gggttattaa cagcttttaa caggtcaatg
     2881 ttagtttctg tttatgtctt aaaaatgttt aaccgcaaag gcgcacgaag ggccttcaca
     2941 aaggcacacc aagtcattga ttagcaggat aaaacctttg tgttccttag tgtattcctt
     3001 tgtgcgcctt tgtggttaaa taaaagactt ttaatacaac ctgaagagaa tttttatggt
     3061 gaataaaggt taactttgct taaaacaatc agttaatagt gaaagaaaaa acgagtatca
     3121 tcccgattgc ctgcgatcat ggcggatatg aactgaaaga gttcataaag atgaacttat
     3181 tgaaagatgg atatgagata aaagattttg gcaccgatgg tccggagagt gtcgattatc
     3241 ccgattttat ccatccgctg gcgaaagggg tcaatgatgg cgtttatccc cttggtatca
     3301 tcatctgcgg gagcgggaac ggcgcccaga tggtggccaa caaatacccg aacgtcaggg
     3361 cggcgctgtg ctggaacgaa gagatcgccc gcctggcgcg gcttcacaac aacgccaata
     3421 tcctttccct tcccggacgg ttcgtttcta aagaagaagg atggaatatg gtgaaggttt
     3481 tcctggaaac atctttcgaa ggcggcagac atcagaagag agtggaaaaa attaatttta
     3541 aattctgaat tttgaatttt taattgaact gttgttgtca tggaattgca gaaaaagttt
     3601 ctgaaagacg cggaaaaagt tgctttcgat ccggagcacc ggcggaagct caatcataac
     3661 atctcgaaat accaggtcaa tgtagaaaaa gggaagaaac agttcaatga tctggagctg
     3721 gcgcggaaga aagccgccaa cacccggcac aagatcctca acgatctcga caagtacctg
     3781 atcgaattcg agagcaattt tgaaaagaac gggggaaagg tgatctgggc ctctacggca
     3841 aaagacgccg ttaaagagat catggccatc atgaagaata caaaggctgt ttcggtggtc
     3901 aaatcgaagt cgatgatgac cgaggagctg gagttgaacg agatactgga aaagaagaag
     3961 atcgaagcca ttgagaccga cctgggcgaa ttcatcgtgc aactggccgg cgaaaagcca
     4021 tatcacatcc tgaccccggc gatgcacaaa tcgaaggaag atgtggcaaa gctgtaccac
     4081 gaaaagttcg gtatgccgct tgattcaacg ccggcagcca tcgcggccta cctgcgcgac
     4141 ttcaaagtga ccgtaatagt cggcttgccg
//
LOCUS       DAOQXS010000263         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21814_length_4166_cov_2.992703, whole genome shotgun sequence.
ACCESSION   DAOQXS010000263 DAOQXS010000000
VERSION     DAOQXS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21814_length_4166_cov_2.992703"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKM34_07890"
     CDS             complement(<1..777)
                     /locus_tag="PKM34_07890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ83548.1"
                     /translation="MTFEQTGLQENIVRATDELGFQQPTPIQERIIPLIINQTNDLIA
                     LAQTGTGKTAAFGLPLLQLTDMDAREIQSIVLCPTRELCMQITGDLKKYSKYIPGFKT
                     VAVYGGADIRAQIKALKNGCQIVVGTPGRVMDLINREVLKLSLIRFLVLDEADEMLNM
                     GFKEDLDIILAGTPQSKRTFLFSATMPDEIASIARNYMNEPEKVSVGKLNQGADNVRH
                     EFYMVQAKDRYAALKRIADIYPNIYGIVFCRTRRETQEVAD"
     gene            complement(945..3539)
                     /locus_tag="PKM34_07895"
     CDS             complement(945..3539)
                     /locus_tag="PKM34_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNQ83549.1"
                     /translation="MNRLYNCVFSILAIVTGLAGCNPEPEQVQPLEVALKSNWSFRQM
                     ETGPWYKAVVPGTVHTDLLLNNIIEDPYYRLNEKQLQWIDKKDWEYRTSFYVDKDVLN
                     NDRVELDFEGLDTYARVFLNGSLILEADNIFRRWTADVKGKIRAGDNELVVLLESPTR
                     KGLEALSAYGFQLAADNDQSENGEMGADRVSPYVRKATYHFGWDWGPRLVTSGIWRPV
                     KLRAWSGARIESVKITTIEITSEMASVEAEVEVEVARAGRYWINVRQRDGVPRDAIPR
                     DAIPGLHLVKIPLTIHNPKTWNPNGLGEQYIYHFDIELTNNDKVVDETEVTAGLRRVR
                     LVQEPDPDGKGRSFYFEVNGKPVFAKGANYIPNDIFLNRVTPDRYEFIVKSAADAHMN
                     MLRVWGGGVYEVDLFYELCDQYGIMVWQDFMFACAMYPGNDEFLENVRLEAIDNVKRL
                     RNHPCLVLWCGNNEIEAAWGPYDENRGWGWKQRYNEEQREIIWQAYDTLFHKILPGVV
                     QQYDPGTPYWHSSPSAGMGELASYETASGDIHYWGVWHGLHALTDFRHYRGRFMSEYG
                     FQSFPEFNSVKKYTLPEDHSIESPVMMAHQRSGIGNLRIRQYMETDYIIPADFEQFLY
                     VGQLLQAEAIKLAIESHRAEMPYCMGSLYWQINDCWPVASWSGIDYYGRWKALHYFVK
                     EAFKPEILVCFEQNDSIKVKIIPDDPGNESFQVKMTLMDFTGKVIWQNSRANIFPNEI
                     SSRPLSEILHNTDPASVVLVSELMRNDEILDRDLHYFVKPKDLKLIDPQIKMEFLERG
                     DDVAVTVSSKSLAKNVFLFYDGEEEQFSDNFFDVLPGESVKITFPKGRSTVQNYRSLK
                     ALHLFMTK"
     gene            complement(3603..>4166)
                     /locus_tag="PKM34_07900"
     CDS             complement(3603..>4166)
                     /locus_tag="PKM34_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83550.1"
                     /translation="NVMLTHLLISSGTINLNYDSAIFRPRIIFISSDSHQGSSYVDYD
                     EFGRYFDYGVSKAIANYSYFKLVLNTYATELSKYINRDGIRAGIHVICPGPVNSNIVK
                     EAPLLLRLILKGIFTIIFKSPAKACKPVVYLAISPDFEGMTNEYLHMFRPKRMDPKVY
                     LPEEGEKLWKRSLELWQSVDPMARIIP"
BASE COUNT         1098 a         1043 c          883 g         1142 t
ORIGIN      
        1 atcggcaact tcctgggtct ccctgcgggt tctgcagaaa acgataccgt aaatattcgg
       61 gtaaatatcg gcaattcgtt tcagcgccgc ataccggtct ttagcctgaa ccatatagaa
      121 ttcatgacgg acattgtcgg cgccctggtt gagtttgccg acagaaacct tttccggctc
      181 attcatatag tttcgggcaa tagaagctat ttcgtcgggc atagtggctg aaaacagaaa
      241 agtacgtttg gattgtggcg taccggccag aatgatatca aggtcttctt tgaatcccat
      301 attcagcatc tcgtctgctt catccagcac caggaaccgg atcagcgaca atttcaaaac
      361 ttcccggttt ataagatcca tcacgcggcc cggtgttcca acaacgatct ggcaaccatt
      421 cttcagggct ttgatctggg cgcggatgtc ggccccgcca taaacagcca ccgttttaaa
      481 tccgggaata tactttgagt actttttcaa atcgcccgtg atctgcatgc acaattcacg
      541 tgtcgggcac aggacaatac tctgtatctc cctggcgtcc atatcggtaa gctggagcaa
      601 aggcaagccg aaggcggctg tctttcccgt tccggtctga gccagggcta tcaggtcatt
      661 ggtttgattt atgatgagtg ggatgatccg ttcctggatc ggggtcggct gctgaaagcc
      721 aagttcatcg gttgcacgga cgatgttttc ctgcaacccg gtctgttcaa aagtcatgta
      781 acggtattta attgtagttc cttcaaggaa ggtaaaatcg gcggcaaagg tagttctatt
      841 ttattgatta tcaagaaaaa taatttggga ttaaacttta tattacagaa ttatgtgagt
      901 ataggattta tcagaaactg atgttttatt agttcgattg attatcattt tgtcataaat
      961 aaatgcaaag ctttaagcga ccggtaattc tgtaccgtac tcctgccctt cggaaaggtt
     1021 atttttactg attcaccggg gagaacatcg aagaaattat cggaaaactg ttcttcttct
     1081 ccatcataaa aaagaaatac attttttgcc aggcttttgg atgaaacggt aacagctacg
     1141 tcatcacccc tttccagaaa ttccatttta atctgaggat caattagttt cagatccttt
     1201 ggctttacga aatagtgaag atccctatcc agaatttcat cgtttctcat cagttccgat
     1261 acgagtacaa cagaagccgg atcggtgtta tgcaggattt cagatagcgg ccgggaagat
     1321 atttcatttg gaaatatgtt ggcccggcta ttctgccaga taaccttgcc ggtaaaatcc
     1381 atcaaggtca ttttcacctg aaaactttca tttcccgggt catccggtat aattttgact
     1441 ttaatggagt cattctgttc aaagcaaacc agaatttcgg gtttaaaagc ttctttcaca
     1501 aaatagtgca gtgctttcca ccggccataa taatcgatcc ccgaccagga agcgaccggc
     1561 cagcaatcgt tgatctgcca gtacaaggag cccatacaat aaggcatctc ggcccggtgc
     1621 gattcgatgg ccagtttgat ggcttcagcc tggagcaact ggccaacgta gagaaactgc
     1681 tcaaaatcag caggaatgat ataatccgtc tccatatatt gcctgatccg caggttacct
     1741 ataccgctgc gctgatgcgc catcatcaca ggtgattcaa tggaatgatc ttccggcagc
     1801 gtatactttt tcactgaatt gaactccgga aacgattgaa agccgtattc gctcataaaa
     1861 cggccacgat aatgcctgaa atcggtaagc gcgtgaagtc cgtgccatac gccccagtaa
     1921 tggatatccc ctgaagcagt ttcgtacgag gccagctcac ccataccggc tgaaggagat
     1981 gaatgccagt agggcgttcc gggatcatat tgctgcacta caccgggcag gattttgtga
     2041 aacaacgtat cataggcctg ccagatgatc tcccgctgtt cctcattata cctttgtttc
     2101 cagccccatc ccctgttttc atcgtacggg ccccatgccg cttcgatctc gttattaccg
     2161 caccataaca ccaggcaggg atggtttcgt aaccgtttca cattatcaat tgcttcgagc
     2221 ctgacatttt cgagaaattc atcatttccc gggtacatgg cgcaggcaaa cataaaatcc
     2281 tgccagacca tgataccgta ttggtcgcat aattcgtaaa acaggtcgac ttcataaaca
     2341 ccaccacccc agacacgtag catattcata tgggcatctg ctgccgattt gacgatgaac
     2401 tcataccggt cgggtgttac ccggtttaaa aatatatcgt tggggatata gttagctcct
     2461 ttggcaaaaa ccggttttcc gttgacttca aaataaaaac tccggccttt gccgtccggg
     2521 tcgggttcct gtaccagcct gacacgacgg agaccggccg taacttctgt ttcatcaacg
     2581 actttatcat tattggtcaa ttcaatgtca aagtgataaa tatactgctc tccgagcccg
     2641 tttggattcc aggtcttcgg gttatggatg gtcaatggga ttttcaccag atgaaggccc
     2701 gggatggcgt ccctcgggat ggcgtcccga gggacgccat ccctttgtct gacatttatc
     2761 caatacctcc ccgcccttgc cacctctacc tctacctccg cttcaacaga tgccatctca
     2821 gaggttattt caatggtcgt tatcttcaca ctttctatcc gcgcaccgct ccaggccctc
     2881 aactttaccg gcctccaaat ccccgaagtc accagcctgg gtccccagtc ccagccaaaa
     2941 tgatatgtcg cttttctcac atacggactg acccggtcag cacccatctc gccgttttcc
     3001 gactgatcgt tgtcggcagc cagttgaaaa ccatacgcac ttaacgcctc caatcctttc
     3061 ctggttggcg attccagcaa aacgacaagc tcattatccc ccgccctgat cttgcccttc
     3121 acatctgcgg tccaccgcct gaatatgttg tcggcctcca ggatcaaaga tccgttaaga
     3181 aaaacacggg catatgtatc cagcccttcg aaatcaagct caacccggtc attattaagg
     3241 acatccttat ccacataaaa acttgtcctg tattcccagt cttttttgtc aatccattgc
     3301 aactgctttt cgttcaaccg ataataaggg tcttcaatga tgttgttaag tagaagatcg
     3361 gtatgtacgg tccccggaac aacggcttta taccacgggc cggtttccat ctgacggaaa
     3421 ctccagttcg actttaatgc cacttccagc ggctgaactt gctccggttc cggattacaa
     3481 ccagccagcc cagtaacaat cgcaagaata gaaaagacac aattatacaa acgattcatt
     3541 ctcagttctc tatttatatt aggcttacaa acgggtttaa taaaaacagt tgcaataatt
     3601 gcttacggga ttatccttgc catgggatca accgactgcc aaagttccag cgaccttttc
     3661 cataatttct cgccttcttc cggaaggtat actttaggat ccatcctttt tggtctgaac
     3721 atgtgaagat actcgttcgt catgccttca aaatccgggg aaatagccag gtaaacaaca
     3781 ggtttgcagg cttttgcggg agatttaaag atgatggtga atatcccctt caaaataagt
     3841 ctcaataata aaggtgcctc cttgacaatg ttggaattga ccggaccggg acaaatcaca
     3901 tggatccctg ccctgatacc atcgcggttt atatacttcg acaattcggt tgcataagtg
     3961 ttaagcacta acttaaaata gctgtaattt gcaatcgctt ttgacacgcc atagtcgaaa
     4021 taccggccaa attcatcata atcaacataa gatgaaccct ggtgggagtc ggaggaaata
     4081 aagatgatcc tgggcctgaa aatagcggaa tcataattca gattgatagt tccggagcta
     4141 atcagcaaat gggtcagcat tacatt
//
LOCUS       DAOQXS010000264         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_21862_length_4160_cov_3.310110, whole genome shotgun sequence.
ACCESSION   DAOQXS010000264 DAOQXS010000000
VERSION     DAOQXS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21862_length_4160_cov_3.310110"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1443
                     /locus_tag="PKM34_07905"
     CDS             280..1443
                     /locus_tag="PKM34_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNQ83551.1"
                     /translation="MLKILVVDDERSIRNTLRDILTYEKYDVSDAESGIVALEMLRQS
                     EFDVILLDIKMPQMDGMEVLDHILAEYEVPVIMISGHGTIETAVDAIKKGAYDYVAKP
                     LDLNRLLITIRNALDKSRLMTETRSLKKRVSKKYEIIGETEVMNGIREMIDRVAPTHA
                     RVLITGDNGTGKELVARQLHEKSLRARGPFIEVNCAAIPSELIESNLFGHEKGSFTSA
                     IKQRKGDFELATGGTIFLDEIGDMSLTAQAKVLRALQENKIMRVGGEKEIPVDVRVIA
                     ATNKNLKDEIAAGRFREDLYHRLSVILIHVPPLRERVDDIPVLAHYFMEEICAEQGKP
                     LMFFTDEAFEELKMMKWTGNIRELRNVVERLIILCDKEITDSDVRRFAQPLVI"
     gene            complement(1448..2611)
                     /locus_tag="PKM34_07910"
     CDS             complement(1448..2611)
                     /locus_tag="PKM34_07910"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HNQ83552.1"
                     /translation="MRAKKLLSRFLLSYCLMALTFCGSEMNAQVRVSKVSDPSQLTGK
                     KGIFYSLPRTKIHINLGIVRTEQIAGPLAEYAKEMLGIDKVVEKNMVTYAIDHVAIMT
                     ESETDPEQLFLVEKEEKAQGEVWIAFGKREPVVALEKFDKASHPDGFTPWNGQHIPLA
                     DPQSLFRKYTDSPVREVIDTIVRKVSIDTLVMVDMICKRTLAEFSDLEKAQEAAARIR
                     QIEQDQYNLLIGYQETAYSIESLQFMYDKLEELRLEYLKLFTGLTLNTLMRYDLPVVP
                     SIGEDDQAYTLGGFSKSTGLVEAEGQNAIILKLISSESALSDLAKKQESFTGGIAYRL
                     PVKVKAAVFNQGKELVSKEIEVLQLGPVISLPSEFKRVEFDTETGNLRSVIIE"
     gene            complement(2629..>4160)
                     /locus_tag="PKM34_07915"
     CDS             complement(2629..>4160)
                     /locus_tag="PKM34_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNQ83553.1"
                     /translation="IDSALFAARPDLVLTGRTLFGHPSAKDQQLSDHYFGSIHERVAH
                     FMRDVEYESHGLGIPLKTRHNEVAPNQYECAPIFEEVNLAVDHNQLLMHLLKKVANHH
                     NFTVLLHEKPFASVNGSGKHNNWSMATDTGVNLLSPGKTAKTNLQFLVFIVNVLKAVH
                     EHAGLLRAGISSAENDNRLGGHEAPPAIISAFIGSHLTKILNEIEQKVRKGRISSDLQ
                     EELSVNISKIPEILRDNTDRNRTSPFAFTGNKFEFRAVGSSDTCARAMIILNTIMADQ
                     LVSFKNDVDKLLEEGVKKDEAIFKVLKRYITESKAIRFEGNSYSEEWVQEAGRRGLIT
                     ARSTPEAFKEYISPKTVKLFNDHKVFTERELLARYDIKLESFTRKLQIESRMMGDLAK
                     NHLIPTAIRYQNMLIENAMGLKQVLDAKTYVKLSKNQVDTIKDISDHVSQVKINVDLM
                     LEERKKANRAGSLEEMTCAYDEKVKPYFDIIRYHIDKLELLVEDNLWPLPKYREIMFL
                     K"
BASE COUNT         1069 a          962 c          985 g         1144 t
ORIGIN      
        1 cagtgattcc atggctgctg gatgcaggac cagttctcct gttttggcta tccaataatt
       61 acaacagtgt ataaaatcgt aattcttatg tatctcatct ggttctccat aaaatcttat
      121 aacgatttgg accttatcgc tcaaggtaat tgcattggcc gacataaata ccggcctgaa
      181 tggagcttta gaagcatcca tttcatcaat gtgatcagca atctcatctc cggtagtcag
      241 catgttgtcg gcatactgca ttccttcctc atctggccta ttctcaaaat actggtagtc
      301 gatgatgaga ggagtatccg taataccttg cgggatatcc tgacatatga aaagtatgat
      361 gtgagcgatg ccgagtcggg tatagtggcc ctggagatgc tcaggcaatc agaatttgat
      421 gttattctcc tggatatcaa gatgccgcaa atggacggta tggaagttct cgaccatata
      481 ctggccgaat atgaggttcc ggtgatcatg atttcgggtc atggaacgat tgaaacagcc
      541 gtcgatgcca tcaagaaagg ggcctatgat tatgttgcca aaccactgga tttaaaccgt
      601 ttgctgatta caatccggaa tgcgctggat aaatcgaggt tgatgaccga aacccggtca
      661 ttgaaaaaga gagtcagcaa aaagtacgag atcatcggtg agacggaggt gatgaacgga
      721 atccgcgaga tgatcgaccg ggtggcacca acccatgccc gggtactgat aaccggtgac
      781 aacggtacag ggaaggagtt ggttgccagg caactccatg agaaaagcct gcgggcacga
      841 ggaccattca ttgaagtcaa ctgtgcggca atcccctccg aactgataga aagtaatcta
      901 ttcggacatg aaaaaggttc atttacttcg gcgatcaagc agcggaaagg ggattttgaa
      961 ctggccacgg gcggaacgat ttttctggac gagataggcg atatgagcct tacggcccag
     1021 gcaaaagtat tgagggcgct gcaggaaaac aagatcatgc gtgttggtgg cgaaaaggag
     1081 ataccggtgg atgtcagggt gattgcggct accaataaaa acctgaaaga tgaaattgcg
     1141 gccggaaggt ttcgtgaaga tctgtaccac cggctgagcg tgatcctgat ccatgtcccg
     1201 ccgctcaggg aaagagtgga tgatattccc gtcctggcgc attattttat ggaagagata
     1261 tgcgcagaac agggcaaacc cctgatgttt tttacagacg aagcttttga agagttgaag
     1321 atgatgaagt ggaccgggaa catcagggaa ctccggaatg tggtagaaag attgatcatc
     1381 ctttgcgaca aggaaattac cgattctgac gtcaggcggt ttgcccaacc gctggtcatc
     1441 tgaagatcta ttcgataatc actgatctta aatttccggt ttcagtgtca aattccaccc
     1501 gtttgaattc ggagggcagg ctgatgaccg ggcccagttg caatacttcg atttcttttg
     1561 agaccagctc tttaccctgg ttaaaaacag cagctttgac tttaacaggc aaacggtaag
     1621 caatgcctcc cgtaaaagat tcctgcttct tcgccaggtc ggacagagcg gattcacttg
     1681 atatcagttt taaaatgatc gcattctgtc cttccgcttc gaccagaccg gtgcttttag
     1741 aaaagccgcc cagtgtatag gcctggtcat cttcaccgat agaagggact acaggaagat
     1801 cgtatctcat cagggtattc agggtcaggc ccgtaaatag cttaaggtat tccaagcgca
     1861 gttcttccag tttatcatac ataaattgca aagactcgat ggaataggcc gtttcctggt
     1921 acccgatcag caggttgtat tggtcctgtt cgatctgccg aatcctggca gccgcttcct
     1981 gcgccttttc caggtcggag aactctgcca gggtgcgctt gcaaatcata tccaccatga
     2041 ccaaagtatc gatacttacc tttcgcacta tggtatcgat cacctctctg acaggcgaat
     2101 cggtatattt cctgaaaagt gactgtgggt cggcaagcgg aatatgttga ccgttccatg
     2161 gtgtaaatcc atccggatga gaggccttat caaatttttc caacgcgacg accggctccc
     2221 tttttccgaa tgctatccag acttccccct gagctttctc ttccttttcg accaggaaaa
     2281 gctgctccgg atccgtttcc gactcggtca ttatggcaac atgatcaata gcataagtta
     2341 ccatgttctt ttccaccact ttgtcgatac ccagcatctc ttttgcatat tctgccagtg
     2401 ggccggctat ctgctcggtt ctgacgatgc ccaggttaat gtggatcttc gtcctgggca
     2461 gggaataaaa aatgcccttt ttacctgtca actgagaagg atcagaaacc tttgaaaccc
     2521 taacctgggc gttcatttcg cttccgcaaa atgtcaaagc catcaggcaa tacgataaaa
     2581 gaaatcggga taacaacttt ttggctctca taatcaagca attgaaatct atttcaggaa
     2641 catgatctcc cggtatttgg gcaaaggcca caaattatct tcaaccagca gttccagttt
     2701 atcaatatga taacgtatga tatcaaaata aggtttcacc ttttcgtcgt atgcgcaggt
     2761 catttcttcc aatgaaccgg cccggtttgc tttttttctt tcctccagca tcaggtcaac
     2821 atttattttc acctgactga catgatcgga aatgtcttta atagtatcca cctggttttt
     2881 agataactta acataggttt ttgcgtccag aacctgttta agccccatgg cattttcaat
     2941 cagcatgttc tgatagcgga tagcggtagg aatcaggtgg ttcttggcca ggtcgcccat
     3001 catcctggat tcaatctgca acttccgggt gaaagattcc agcttgatat cgtaccgtgc
     3061 caataactcc ctttcagtaa agactttatg gtcattaaac aatttaactg tcttaggtga
     3121 aatgtattct ttaaaagctt ccggggtgga acgcgccgtt ataaggcctc tccttccggc
     3181 ttcctgcacc cattcttcgc tatagctgtt cccttcaaaa cggatagctt tggattctgt
     3241 tatatatctt ttcagtacct taaagatcgc ttcatctttc tttacccctt cttcgagcag
     3301 tttatcgaca tcatttttaa aactaacaag ctggtcggcc atgatagtat tgaggatgat
     3361 catagccctg gcgcaggtat ctgacgagcc cacggccctg aactcaaatt tattgcccgt
     3421 aaaggcaaac ggagaggtac ggttgcggtc ggtattgtca cggaggatct ccggaatctt
     3481 tgaaatattg acgctgagtt cttcctgtag atcactggaa attcgacctt tcctgacttt
     3541 ttgttcaatt tcgttaagga tctttgtcag gtgagaaccg ataaatgctg aaatgatggc
     3601 cgggggcgcc tcatgaccac cgagcctgtt atcgttttca gccgacgaaa tgccggccct
     3661 caacagccct gcgtgttcat ggacggcttt cagaacatta acgataaaaa cgaggaactg
     3721 gagattggtt ttggccgttt ttcccggcga aagcaggtta actccggtat cggttgccat
     3781 cgaccagttg ttatgctttc cggacccgtt cacgctggca aacggttttt catgcaacaa
     3841 cacggtaaag ttgtgatgat tggctacttt tttcaggagg tgcatcagga gttgattatg
     3901 atccaccgcc aggttgacct cctcgaaaat cggagcacat tcgtactgat ttggtgcaac
     3961 ctcattgtgc ctggttttca agggtatgcc aagcccgtgt gattcatatt ccacatctct
     4021 catgaaatgg gctacccttt cgtggatact tccgaaataa tgatccgata attgctggtc
     4081 cttggcagaa gggtggccga agagcgttct tccggtcagg accaggtcgg gccgggcggc
     4141 aaataacgcc gaatcgatca
//
LOCUS       DAOQXS010000265         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22012_length_4137_cov_3.970848, whole genome shotgun sequence.
ACCESSION   DAOQXS010000265 DAOQXS010000000
VERSION     DAOQXS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22012_length_4137_cov_3.970848"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..768
                     /locus_tag="PKM34_07920"
     CDS             367..768
                     /locus_tag="PKM34_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNQ83554.1"
                     /translation="MTEENRNKLIHYWIDGSDEDFDTMLTLFDSKRYSWALFIGHLML
                     EKLFKAYFVKMNSDYPPYIHNLLRLAELSGLLLTQDQKERLLTITAFKINARYDDYKT
                     SFKLKCTPEFTARWLDELKEIRTWIKTLLIS"
     gene            741..1055
                     /locus_tag="PKM34_07925"
     CDS             741..1055
                     /locus_tag="PKM34_07925"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ83555.1"
                     /translation="MDKDIADILEKYLNLVRYRFSGIETAILFGSYAKGKAGSYSDID
                     VALIFSDLKETERFDLQVQLMLIAAQIDSRIEPHPISSAEFKSENHFVHEIQRTGIEI
                     KT"
     gene            complement(1487..2143)
                     /locus_tag="PKM34_07930"
     CDS             complement(1487..2143)
                     /locus_tag="PKM34_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNQ83556.1"
                     /translation="MKIAVLGTGMVGEAIGSKLIELGHSVRMGSRTATNEKALAFVAK
                     HPGGNASAGTFAGAAAFGEVIFNCTKGTESMNILRSAGIENLDGKVLVDVANPLDFSN
                     GMPPSLSVCNTDSLGEQIQREFPGVKVVKSLNTMWCGLMVNPGMLNGGDHHVFMSGND
                     AGAKAVVSGLLQSFGWRSENIIDLGDITTARGTEMMLPVWLSLYMAKNHGAFNIRIMG
                     "
     gene            2346..2570
                     /locus_tag="PKM34_07935"
     CDS             2346..2570
                     /locus_tag="PKM34_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83557.1"
                     /translation="MKNDRLENRMRMQHNKEKISDEKLLMGATCDKFPVVMDNGKTIV
                     YITDKSKEAETRLRYELLKKSRFPMQSDFK"
     gene            complement(2756..3919)
                     /locus_tag="PKM34_07940"
     CDS             complement(2756..3919)
                     /locus_tag="PKM34_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83558.1"
                     /translation="MYYSREIEKEVLKSIANNPVTAITGPRQCGKSTLAKHIAELSGK
                     DTLYLDLEKPSDLSKLDDPEWFLGLQKEKLICLDEIQRKPELFPLLRSLVDEWAGNGH
                     FLVLGSASRELLRQSSESLAGRISYKRLTPFLLSELNNEIQLPDYLSRGGFPRSVLAR
                     DNEQSLRWREDFISTFLERDLLLWSGFSPVTMRKLWQMLAHLNGQVVNFSLLGSSLGV
                     SHTTVRNYVELLQSTFMLHLLQPIASGTGKRLVKSPKVYLSDTGINNALLKIADFEQL
                     AGHPSFGTAWETTVLNNLTGTFPHFNFYFYRTGHGAEIDIVVENGEKRIAVECKASLS
                     PRLSAGTFIAIADVRPLVCLVVTPAEKGWPMKDGVEVVSIGEAIRKIRDLLSM"
BASE COUNT         1210 a          925 c          863 g         1139 t
ORIGIN      
        1 aactcatgcg tgtgatcccg agtgctcggg actgaaccaa ctgagctaac atcccataaa
       61 gaactcatgc gtgtgatccc gagtgctcgg gactgaacca actgagctaa catcccgtta
      121 ggggtgcaaa gttataaaaa agatgttatt accggaacat aatttctcgc agattttcgc
      181 ggatttagac gcagattccc gctgataata atcattacaa taaatttctg cgaaagcagc
      241 gctttgattt gcgtgaattg cattccagtt gtcagcatct ttttaagttg aattgtcaat
      301 gtcaacgggt tgaaatgatt atggttaatt acttattgga ttttatgtat ctttgaagta
      361 atgaaaatga cagaagaaaa ccggaataaa cttattcact attggattga tggttcggac
      421 gaagattttg atacgatgct gaccttgttc gattcaaagc gatatagttg ggcactattt
      481 atcggacatt taatgcttga aaaacttttt aaggcatatt ttgtgaaaat gaattcagat
      541 tatcctcctt atattcacaa tctgttaaga ttggcggagc tttcaggatt attattgacc
      601 caagaccaga aagaaagact acttacgata actgcattta aaatcaatgc cagatacgat
      661 gattacaaga ccagttttaa gctgaaatgc actccggaat ttacagcaag gtggctggat
      721 gaattaaaag aaataagaac atggataaag acattgctga tatcttagaa aagtatttaa
      781 atcttgttcg atatagattc agcggtatag aaacagctat cttgtttggt tcttatgcca
      841 aagggaaggc cgggagttat agtgacatag atgtcgctct catcttcagt gatttaaaag
      901 aaacagaacg atttgattta caggtacagt taatgctgat tgcagcacaa attgattccc
      961 gaattgaacc tcacccgatt tcatctgctg aatttaaatc tgaaaatcac tttgtccatg
     1021 aaatccaaag aacagggatt gaaattaaaa cctgacccga cattattttc aagtaaaaga
     1081 aattcatcct ttactttatt ttctcgcaga ttttcgcgga tttagacgca gattcccgct
     1141 tatttcccat cgaccatcga caatcgacca tcgacaatcg aatatcttct cttccatttc
     1201 gccatcaaca cactaataaa cattatcatt cacaatgggt attttttact ctttttttat
     1261 atggttgagg ttcggggaaa ctcacaaaaa aagatgtata aaatgaaaat gtcagggcat
     1321 ggaaagggtg aaaaattaaa aatgtcgggg caaagaggtt gtagtatatg actggcctac
     1381 tttacaccga aataggtggc cttgtttgtc cgaaataata agattaatcc tgtaagaaat
     1441 gaggtcaccg gttcgaattc agcacatccc acccctcatt cccgatctac cccataatcc
     1501 tgatgttgaa ggcgccatgg ttcttggcca tatagaggct tagccataca ggaagcatca
     1561 tttcggtgcc gcgggcagtg gtgatgtcgc ccaggtcgat gatgttctcg ctcctccagc
     1621 caaaactctg cagcaggccg gatacaacgg ccttagcccc ggcgtcgttg ccgctcatga
     1681 acacatggtg gtcgcctccg ttcagcatgc cgggattgac catcagtccg caccacatag
     1741 tgttgaggct ctttacgact ttcaccccgg gaaattcccg ctggatctgc tccccgagcg
     1801 aatcggtgtt gcaaaccgat aacgatggcg gcatgccgtt ggagaaatcg agcggattgg
     1861 ctacatcgac cagcactttg ccgtcgaggt tctctatgcc ggcactgcgg aggatattca
     1921 ttgactccgt ccctttcgtg cagttgaaga tgacctcgcc aaaagcggct gcgccggcaa
     1981 aagtccccgc cgaggcgttg cctcccggat gctttgccac aaaggccaat gctttctcgt
     2041 tggttgctgt tctcgagccc atcctgaccg aatggccgag ctctattaat tttgaaccga
     2101 tggcctctcc gaccatcccg gttcccagta cggctatttt catgagttaa agtttcacag
     2161 ataaacaacc gccaccggaa aaggttcagc aggccacaat caacacgtta agtaatattt
     2221 cattccattt gaccgtgatt taaaataatg ttaataaata aaattcagta aatatgataa
     2281 tgtaatatat attattcgta tcttgtgaga taatttaaat cacatatatt catataaata
     2341 tcaagatgaa aaacgaccgg ctggaaaaca ggatgagaat gcaacacaat aaagaaaaga
     2401 tctccgatga aaaattactg atgggggcaa catgtgataa attccctgta gtcatggata
     2461 atggcaaaac gattgtatat atcaccgata aaagcaagga agccgaaacg agactacggt
     2521 atgaattact gaagaagagc aggttcccga tgcagtccga ttttaaatga acgcttcagc
     2581 caatgcgatc accaaccgca tgacaggtca ttcgaataaa ttcagattcg ttgaaatgtc
     2641 gaatgaagat gaggcgaaca aggagatcaa ccgcagctgt tttatactca ttctttgcag
     2701 gtttacaaga gcaaagctgc tacgaatgag ttatgccgaa gggggaacat aatggtcaca
     2761 tactgagcag atccctgatc ttccggatcg cttcgccaat actgaccact tcaactccat
     2821 ccttcatcgg ccagcctttc tctgccggag tgactacaag acataccagc ggccggacat
     2881 ctgcaatagc gatgaatgta cctgcactaa gtctgggcga caaacttgcc ttgcactcga
     2941 cagcaattct tttttcaccg ttttcaacaa caatatcaat ttcagcccca tggccggtcc
     3001 tgtagaaata aaaattgaaa tgaggaaatg tccctgtcag attgttaaga acagtggttt
     3061 cccaagccgt tccgaaagaa gggtggccgg ccagttgctc aaaatcggca atcttaagca
     3121 aggcattatt tatccctgtg tcgctgagat aaactttggg cgactttacc agccgtttgc
     3181 ctgtgccaga tgcaataggc tgaagtaaat gaagcatgaa tgtcgattgc agcagctcca
     3241 cgtaattcct tacggttgta tgacttaccc caagcgaact acccagcagt gaaaaattta
     3301 caacctggcc attcaggtgg gcaagcattt gccataactt acgcatggta accggtgaaa
     3361 agcccgacca taacagaaga tccctttcca ggaaagtgga gataaaatcc tccctccatc
     3421 tcaatgattg ttcattatcc ctggctaaaa cgcttctggg gaaacctccc cttgacaggt
     3481 aatccggtaa ttggatttcg ttattcagct cacttaacag aaatggcgtc aatcttttat
     3541 aagagatcct ccccgcaagg ctttccgagc tctgccggag cagttcccgt gaggcagagc
     3601 ccaataccag aaaatgtcca ttcccggccc attcatcaac taatgaacgc agtaatggga
     3661 acagctccgg ctttctctgt atttcatcca ggcaaattaa tttttctttc tgcaatccta
     3721 aaaaccattc cggatcatcc agtttactga gatcagatgg cttctcaagg tcaaggtaaa
     3781 gagtatcctt cccggaaagc tccgcaatat gctttgccag cgttgatttt ccgcattgcc
     3841 tgggccctgt aattgcagta acgggattat tagcaatgct ttttaagacc tctttttcta
     3901 tttcccggga ataatacatg gatgattcta ttaccgtgca aatttaaagt ataattttca
     3961 tattgcacgt attattttca tattttatag ttggaggcac tggcataccc gtgcaaactg
     4021 acctgcgttt gtctttaccg gcagcttgca tttgcatccg gccgtcagca tccgatgcaa
     4081 cggtgatcag tttttgtttt accttcgcct ggctacaaaa tcaggacatg caccgcc
//
LOCUS       DAOQXS010000266         4116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22162_length_4116_cov_3.664122, whole genome shotgun sequence.
ACCESSION   DAOQXS010000266 DAOQXS010000000
VERSION     DAOQXS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22162_length_4116_cov_3.664122"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKM34_07945"
     CDS             <1..462
                     /locus_tag="PKM34_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNQ83559.1"
                     /translation="AIAFEYLKAHTDSLGIDPDVFTNWNVHIHVPEGATPKDGPSAGI
                     TMLTALASAFTQRKIRPELAMTGEITLRGKVLPVGGIKEKILAAKRAKIKNIILSQEN
                     RKDIEEIKKEYVEGLNFIYIDKMIDVIAHALLPDKIKNPLDFRIKPETFSS"
     gene            522..2162
                     /locus_tag="PKM34_07950"
     CDS             522..2162
                     /locus_tag="PKM34_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ83560.1"
                     /translation="MRRLAFYGLLLAMISSCAEQKPDESGLTVFRYNEFSNISSLDPA
                     FAKDQASIWACNQIYNGLLQLDEDLNILPCIAHSWNIDTTGRIYTFFLRNDVFFHDHP
                     AFPGGIGRKVTAHDFVYSLKRLQDPKLASPGIWVLNKLEKEDGEPAIAALNDTILQIR
                     LKEPFSPFPGILTMIYCSVVPKEVVAEKGIEFRIHPTGTGPFRFKMWKENVKLVLVKN
                     TGYFEKDSNQQRLPYLDAIAISFLIDKQAAFLEFIKGNLDFISGLDQSYKDELLTKDG
                     HLKAGYQDKFMMMTEPFLNTEYVGMLVDSLTDAGRNSPLMNKKVRQAINYGIDRRKMI
                     RYIRNNIGVPGLHGIIPPGLPAFDSSEVFFDYDPQKAQALIREAGYGPANPLPAITLH
                     TTAEYTDIFKYMQHQLSSLGISVKIEVNPAATLMQLKAYSRINFFRASWIADYPDEEN
                     YLSLFVSTNAAPAGPNYTRYNNPVFDSLYKASQLVGDPVIRKDIYRQMNGLVMEDAPV
                     IILYYDQVVRFSWTYIQNLGTNPLNMLNLKSVKKIQEE"
     gene            2223..2804
                     /locus_tag="PKM34_07955"
     CDS             2223..2804
                     /locus_tag="PKM34_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83561.1"
                     /translation="MALFNNIRLRIAGYILQKGITSLKRNKKLVSLNTAGSVGLLFEL
                     TDHSVYYAIQDFFQKLQENKIKVKALGYSGTKNATSQFLPVLSFDFISGKDLNWLLIP
                     KAKCVTDFIETDFDICINIASEDIFPLKYIAGMSKAKLKAGAYNNVAADDNFKALSQI
                     YDIMLKSEPIHNQISFLGIINEYLSILNSKGNV"
     gene            2797..3696
                     /gene="dapA"
                     /locus_tag="PKM34_07960"
     CDS             2797..3696
                     /gene="dapA"
                     /locus_tag="PKM34_07960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNQ83562.1"
                     /translation="MYDYLKGTGVAVITPFHDSGNIDFGSFEKIIEHLITGGINYIVA
                     LGTTGESAALSKDEKVAVLDFVIDTVNKRVPVVAGIGGNNTQEVINTIKATNFEGIEA
                     ILSVCPYYNKPQQKGLYNHYKAISGASQVPVILYNVPGRTSVNITAETTLKLAADFTN
                     IIGIKEASGNLMQCMEIINNKPSGFYVISGDDGLTLPLISLGADGVISVVANAFPKQM
                     TEMVNLCLRGKFTKARALHYNLLGFTNALFMDGNPSGIKAAMELLGLCKSNVRLPLAK
                     VNKNVHSMIAEHTDFLVKNQVEL"
     gene            3720..>4116
                     /locus_tag="PKM34_07965"
     CDS             3720..>4116
                     /locus_tag="PKM34_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83563.1"
                     /translation="MNKFRFYNTVLILLLFAFNISTFSQNSDSKSFLDSLYNNEIEQY
                     FGFDLRQAQGLEYFGKFISIDKVDNNGRVFAYANREGFSKFLETGLSFDLLPHPGDFS
                     GYLNMKEKVDIREISEWDFYPTYDAYIDMM"
BASE COUNT         1246 a          885 c          857 g         1128 t
ORIGIN      
        1 gctattgctt ttgaatacct gaaagcacat accgacagcc tgggaatcga tcccgatgta
       61 tttaccaact ggaatgtgca catccatgtc ccggaaggcg caacgccaaa agatggacca
      121 tcggccggga ttacgatgct aaccgccctg gcttcggcgt ttacgcagcg aaaaatcagg
      181 cccgaactgg ccatgacagg tgaaattacc ttacggggaa aggttctccc ggtaggagga
      241 atcaaggaaa agatcctggc tgccaaaagg gcaaaaatta aaaacatcat cctttcccag
      301 gagaacagga aagatattga ggagatcaaa aaagaatacg ttgaagggtt aaatttcatt
      361 tatatcgata aaatgattga tgtcatcgca catgcccttc tgcctgataa aataaaaaat
      421 ccgctggact tcaggataaa gcctgagaca ttttcatcct gataattgta aaggtctcag
      481 gggccgactt ttttcttatc attgccataa aatacctgga gatgagaaga ctggcatttt
      541 atggtctgtt attggcaatg atttcctctt gcgcggaaca aaagccggat gagtccggct
      601 tgacggtatt caggtataat gaattctcta atatcagttc actcgatccg gcttttgcaa
      661 aagatcaggc aagtatctgg gcatgtaacc aaatctacaa cgggctgttg caactggatg
      721 aagatctcaa tatccttcct tgtatcgctc actcctggaa tatagataca accggccgga
      781 tctatacttt ttttctgcga aacgatgttt ttttccacga tcaccctgca tttcccggcg
      841 gaattggcag gaaagtgact gcgcatgatt ttgtatatag cctgaagcga ttgcaggatc
      901 caaagctggc ctctcccggc atctgggtac tgaacaaact tgaaaaggaa gatggagaac
      961 cggctatagc cgctttaaat gatacgatcc tgcaaataag gctcaaagaa ccattttcac
     1021 cgtttcccgg catcctgacc atgatttatt gcagcgttgt accaaaagaa gtcgttgctg
     1081 aaaaagggat tgaattcaga atacacccga caggaaccgg ccctttccgt tttaaaatgt
     1141 ggaaagaaaa tgtcaaactg gttttggtaa agaacaccgg ttattttgaa aaggattcaa
     1201 atcaacaaag gttaccttat cttgatgcca tagccatatc atttctgatt gacaaacagg
     1261 ccgccttcct ggagtttatc aaaggcaacc tggatttcat ttccggtctc gatcagtctt
     1321 ataaagatga acttttaacc aaagacggac acttaaaagc cggatatcaa gacaagttta
     1381 tgatgatgac ggaacccttt ctcaacactg agtatgtggg aatgctggtc gacagtctta
     1441 cagatgccgg acggaacagt ccgttgatga ataaaaaggt caggcaggcc attaattatg
     1501 gcatcgaccg gcgaaagatg atccggtaca tcaggaacaa cattggcgtc ccggggcttc
     1561 atgggattat acctcccggt ctgccggcat ttgactcaag tgaggtattt ttcgactacg
     1621 atccgcaaaa agcacaggct ttaatccgtg aagccggtta cggcccggca aatcctcttc
     1681 cggcaatcac cctccatact acggcagaat ataccgatat attcaaatat atgcagcatc
     1741 aattaagcag tttgggtata tccgtaaaaa ttgaagtaaa tccggccgca acgcttatgc
     1801 aactaaaagc ctattccagg atcaattttt tcagggcctc atggattgcc gattatcctg
     1861 acgaagagaa ttatctctcc ttgtttgtca gcacgaacgc tgctccggcc ggccccaatt
     1921 atacccgata caacaatccg gtatttgatt ctctctataa agcttcacag ctggtcgggg
     1981 atccggtgat aagaaaggat atttaccggc aaatgaacgg tttagtgatg gaagatgcgc
     2041 ctgtgatcat tctttattac gatcaggtcg taagattttc atggacttat attcaaaatc
     2101 ttggcacaaa tcccctgaat atgctgaatt tgaaaagtgt taaaaaaatt caggaagaat
     2161 gacggctggc tgatgtacct gatcaaaatc ttgctacttt tacatctctt catcttgaga
     2221 tcatggcttt gtttaacaat ataagactca gaatagccgg ttatattctc caaaaaggga
     2281 ttacatcgct aaaaaggaat aaaaaactgg tcagcctgaa tacagcaggt tctgtcggtc
     2341 ttttattcga actcaccgat cattcggtat attatgccat tcaggatttt ttccagaaac
     2401 tgcaggaaaa taaaataaaa gtcaaagccc tgggatattc cgggacaaaa aatgccacca
     2461 gccagtttct gccggtctta tcattcgatt ttatcagtgg taaagatctg aactggcttt
     2521 tgatcccaaa agcaaaatgt gttacagact ttatcgaaac agattttgac atctgtatca
     2581 atattgcttc ggaagatatt ttccctttga aatatatcgc gggaatgtcg aaagccaaac
     2641 ttaaagccgg agcatataac aatgtagccg ccgatgataa ttttaaggcg ttatcacaga
     2701 tctacgacat tatgctaaag tcggaaccca ttcataacca gatatctttt ctcggcatta
     2761 tcaatgaata tttatcaatt ttaaattcaa agggcaatgt atgattacct aaaaggaacc
     2821 ggagttgcgg ttatcacacc cttccatgat tccggaaata tcgacttcgg ttcatttgag
     2881 aaaatcatag aacaccttat aacaggtggt attaattaca tcgttgcact tggaaccacc
     2941 ggagaatcag ctgctctgtc aaaagacgaa aaagttgctg tgctggattt tgtcatcgat
     3001 acagtgaaca aacgggtgcc tgtcgtcgcc ggaattggcg gtaacaatac ccaggaggtg
     3061 atcaatacga tcaaagccac caattttgaa ggaatagagg ctattttatc cgtttgccct
     3121 tattataaca aaccgcaaca gaaaggtctt tacaaccatt ataaagctat atccggtgcc
     3181 agccaggttc ccgtcatact atacaatgtc ccgggaagaa ccagcgttaa catcactgct
     3241 gaaaccacac ttaaattggc tgccgacttc accaatatca tcggtatcaa ggaagcttca
     3301 ggcaacctta tgcaatgtat ggagatcatc aacaacaagc catctggttt ttacgtcatt
     3361 tcaggagatg atggcctgac ccttccattg atctcgctgg gtgccgatgg cgtgatttcc
     3421 gttgtcgcca atgccttccc aaaacaaatg accgaaatgg tcaatctgtg cctgagagga
     3481 aaatttacca aagccagggc cctccattat aatttattgg gatttaccaa tgcactgttt
     3541 atggatggta acccgtcagg aattaaagca gccatggaat tactaggtct ttgcaaaagc
     3601 aatgtaaggt tacctcttgc caaggttaac aagaacgtgc attctatgat tgccgaacat
     3661 accgattttc tggtcaaaaa ccaggtagaa ctatagattg attttttatt attcacccga
     3721 tgaacaagtt cagattttac aacaccgttc ttattctgct gttgtttgca ttcaatatca
     3781 gcacgttcag tcaaaattcc gacagtaaat cattccttga cagcctttat aataatgaaa
     3841 tagaacaata cttcggtttt gacctccggc aagctcaagg tttggagtac ttcggtaaat
     3901 ttatttctat tgataaagta gacaacaacg gacgagtttt tgcctatgcc aacagggaag
     3961 gattcagcaa atttctggaa acgggtttgt catttgattt gttgcctcat cccggcgatt
     4021 tctccggata tctgaacatg aaagaaaagg ttgacatccg cgagatttcc gaatgggatt
     4081 tctatcctac ctacgatgcc tatattgaca tgatga
//
LOCUS       DAOQXS010000267         4115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22170_length_4115_cov_3.253695, whole genome shotgun sequence.
ACCESSION   DAOQXS010000267 DAOQXS010000000
VERSION     DAOQXS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22170_length_4115_cov_3.253695"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..416
                     /locus_tag="PKM34_07970"
     CDS             126..416
                     /locus_tag="PKM34_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83564.1"
                     /translation="MEDQTQFQNQNQNPYQNYNAMQNQQPIPVGDWFVTILITAIPLV
                     NIIMLFVWGFSSSTHPSKANWAKASLIWLAIVIVLYIIIFATIGAAMFSAYR"
     gene            complement(455..2701)
                     /locus_tag="PKM34_07975"
     CDS             complement(455..2701)
                     /locus_tag="PKM34_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83565.1"
                     /translation="MKNSRGHFRAGLKRTAIVLLIIAITVGWCELTHNTITSEAINLI
                     LLKNSHAYKEIELFGERMIINQGRRVNKLEDDFDRAYNHYGVNRGFFCKEYLLTPWVV
                     NLYKKHHTPPPASFDMTDFNSAYEWAYSGSVQKNPGDFYLGNEPWNWEGAVRSYDYSM
                     ISKEAAYERLGHVIHMLQDICQPDHFRRIDHPGSSFSTEELDELMKNIRKIRIIPDIV
                     WRAAGVKIPEPVPGAVGFEKLADLYEDARTHELSGLSIRDWKNVGLADGKLFNYFDSA
                     GQGSEYFRSRFGQPALDTALGLHALPVPVNYNIRAASLNTRWIPPLSVKVKIDHVPII
                     PFIVWDMPSNYPNINGYIKLSGNCTKISVEFSAGLIQLFHDIVRHPPYASKINIYQKN
                     KDGQVLTGLSYEAMWEETDDDRNRKFLKTNAEPGLFQACRQTYIEVTFGPSLSVGELK
                     QATQINPESVRVKVGVRNKSEITVDMVLKEGSDNDGSSPPVFTGSFVPPLSWAGSGDG
                     EKFQGYAKIIAEDIFNHYDYGGRAGANSGKILDALPETKARVTDFKPPYPWADYTPGW
                     DETHNDIGSFVLPPPNIEKCRIYTIGGKEFQQYIGTWDDKGCPFPEFIALDRFGGMLL
                     YDRLAAMEVEIYPEVLKIDQASVSVEIGVNGSLVVYTLNNGIRDFQVQDNGFSLKFQP
                     DLSWKSVEDAVKYGYVRIFLNDNSGHRIDANPATVPVIRSRADWDYYEEGPDLNHQGH
                     VFIEEKPE"
     gene            complement(2691..>4115)
                     /locus_tag="PKM34_07980"
     CDS             complement(2691..>4115)
                     /locus_tag="PKM34_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83566.1"
                     /translation="YISQPDKVMATFLSNAPAPVDVYILGVFEGENGIRVYTDPGYKM
                     YPPVTLLPGVPFNLNRFNLEQVFDEEHLVCQGVTLSELIHGQGLPEDNYTICLQAFDY
                     LTGETVSSESPLGCSNPFTVSDLEPPVILFPVSGQDIFAGDFQNINFVWTWPPGAPNS
                     TRFALKIIEVLPADRNINDAFRSATQPVFFEKIIASTNYLYGPSDPRLVPGKTYAFSI
                     TASDPLQKSFFRNNGESEIARFNFLPEEKPIPASPIEWDVVSSRIIYYNVTSDTLSED
                     GAPVSGQYAVKLDFRGNGDIIFQTDEKGETQTIACKMTPSGNLTWDNDRGMIPVPVLV
                     SLSALKDPTSLIVTGDTWSKISVTDGEMNRNIDELILNFKGDFEALTVLQTGEEELIC
                     IHTEVYNRIEPTDYSKDFYKNPNVTDGPFNWEKYLSGTVYFNIHLKCLERAEMILLPV
                     FKDQAPKSFKMERRIQNENKPDEK"
BASE COUNT         1074 a          999 c          892 g         1150 t
ORIGIN      
        1 taccctgtca agatatttct ttcctgaatt aaaataaatt tatatacctg gagctcggaa
       61 accagccatc aaacggggcg gaaccgaaaa gccacacgag taggacaacc aaaatccaaa
      121 ctaaaatgga agaccagacc caattccaga accagaacca gaatccttac cagaattaca
      181 atgccatgca gaatcagcaa ccgatcccgg tcggcgactg gttcgtgacc attttgatta
      241 ctgcgatccc gctcgtcaac atcatcatgc tgttcgtctg ggggttcagc agcagcaccc
      301 atccgagcaa agccaactgg gccaaggcat ccctcatctg gttggctatt gttatcgtgc
      361 tttatatcat catttttgcc acgataggcg cggctatgtt cagcgcttac agatgataaa
      421 accggccggg gaaccaaaat tacatctgta attgctattc aggcttctct tcaatgaaga
      481 catgaccctg atgatttaaa tcgggccctt cttcgtaata gtcccaatct gccctggacc
      541 gtataaccgg aacagtggca ggattcgcat ctatcctgtg accggagttg tcgttcagga
      601 atatcctgac ataaccgtat tttaccgcat cttcgaccga tttccagctt aaatcgggct
      661 gaaacttcaa ggaaaaacca ttgtcctgca cctgaaaatc cctgatgccg ttgttaagcg
      721 tataaactac cagacttccg ttaacaccga tttcaacact gactgatgcc tggtctattt
      781 taaggacttc gggatatatt tctacctcca ttgccgccag gcggtcatat aatagcatcc
      841 ctccgaaacg gtccagtgca atgaattcag gaaacggaca ccctttgtcg tcccatgtgc
      901 cgatgtattg ctgaaattct tttcctccaa tagtataaat cctgcatttc tcaatatttg
      961 gcgggggtaa cacaaatgat ccgatatcgt tgtgtgtttc atcccaaccg ggcgtatagt
     1021 ccgcccatgg atacgggggc ttgaaatcag tcacccgggc cttcgtctcc ggcaaggcat
     1081 ccagaatttt gccgctattt gcacctgccc ttcctccgta atcgtaatga ttaaatatat
     1141 cctccgcaat tatttttgca tacccctgga atttttctcc atcacctgaa ccagcccatg
     1201 acaagggagg aacgaaactt cctgtaaaaa caggcggtga tgatccgtca ttatcgctgc
     1261 cctctttcaa taccatgtca actgttattt cagatttgtt tctgacccct acttttaccc
     1321 tgaccgattc cggattgatc tgcgtagcct gctttaattc tcccaccgaa agagatgggc
     1381 caaaggtcac ttcaatgtag gtctgacggc atgcctggaa tagtccgggt tcagcatttg
     1441 tcttcaagaa ttttctgttc cggtcgtcgt ctgtttcttc ccacatggcc tcataactta
     1501 atccggtcaa cacctgcccg tctttatttt tctgatatat gttgatctta ctggcataag
     1561 ggggatgcct gacaatatca tgaaataact ggatcaggcc ggcggaaaat tcgacggaaa
     1621 tttttgtgca attgcccgaa agttttatgt agccattaat attcgggtag ttcgacggca
     1681 tatcccagac aataaagggg attatcggaa catgatctat ttttacttta actgataaag
     1741 ggggaatcca tcttgtattt agtgaagcgg cccggatgtt ataatttaca ggaacaggga
     1801 gtgcatgaag ccccaaggcg gtatccaaag ccggttggcc aaacctggag cgaaaatatt
     1861 cggagccctg gccagccgaa tcgaaataat tgaaaagttt tccgtcagcc aatccgacat
     1921 ttttccagtc cctgatactc agcccggaaa gttcatgtgt cctggcatcc tcataaagat
     1981 cggccagctt ctcaaatcca acagcaccgg gaactggttc cgggatttta acccctgctg
     2041 ccctccagac gatgtcaggt ataatccgga ttttgcggat gtttttcatt aattcatcca
     2101 gttcttcagt gctgaaactg cttccgggat ggtcaatacg ccggaaatga tcgggctggc
     2161 atatgtcctg caacatatgt atgacatggc cgagacgttc atatgccgct tccttggaaa
     2221 tcattgaata atcataagag cggacagccc cttcccagtt ccatggctca ttacccaggt
     2281 aaaaatcacc gggattcttc tggacagatc ccgaataagc ccattcataa gcgctgttga
     2341 agtccgtcat gtcaaaactg gctggcggtg gcgtatgatg ttttttataa agatttacta
     2401 cccagggagt aagcaaatat tctttgcaaa aaaatccccg gttaactcca taatggttat
     2461 aggccctgtc gaaatcgtcc tccaatttgt tgacccttcg accctgattg ataatcatcc
     2521 gctccccgaa taattcaatt tctttataag catggctgtt tttgaggagg atgagattga
     2581 tagcttccga ggtgattgta ttatgagtca gttcgcacca tcccacggtt attgcaatga
     2641 tcaaaaggac gattgcagtc ctctttaatc cggctctgaa atggcccctg ctatttttca
     2701 tctggcttgt tttcattttg tatcctcctt tccattttaa aagactttgg cgcttgatct
     2761 ttaaatacag gcaacaggat catttcggct ctttcaagac atttaagatg aatattgaaa
     2821 tacacagtgc cagacaggta tttttcccag ttaaatggcc catcggtcac attcggattc
     2881 ttataaaaat cttttgaata gtcagtcggt tcaatcctgt tgtaaacctc agtatgaatg
     2941 catataagtt cttcttcacc ggtttgtaaa acagtcaacg cctcaaagtc gcctttgaaa
     3001 ttcaggatca gctcgtcaat attccgattc atttcaccat ccgtgacact tattttactc
     3061 caggtatcac cggtcactat taaactggtg ggatctttca aagctgacag gcttaccaac
     3121 acaggtacag gaatcatgcc tctatcattg tcccatgtta aattgcctga aggagtcatt
     3181 ttacaggcga tcgtttgcgt ttcccccttt tcatcggtct ggaaaatgat atctccattt
     3241 cctctgaaat caagctttac agcgtattgt ccggatacgg gggctccgtc ttcggataaa
     3301 gtatccgagg tgacgttgta ataaatgatc cggctggata caacatccca ttcaatggga
     3361 gatgccggaa tgggtttttc ttccggaaga aaattaaaac gggcgatttc actctccccg
     3421 ttattccgga aaaatgattt ttgaagcgga tcggatgctg taatggagaa cgcataagtt
     3481 tttccgggta ccagcctggg atcggatggc ccgtaaagat aattcgttga agcaatgatt
     3541 ttctcgaaga ataccggctg tgtggccgat ctgaaagcat cattgatatt cctgtcggcc
     3601 ggaagtactt cgatgatttt taaggcaaac cgggttgagt tcggtgcccc gggaggccag
     3661 gtccacacga agttaatatt ttgaaaatcg cctgcgaaga tatcctgtcc ggatacggga
     3721 aacagaatca ccggtggttc aaggtccgat accgtgaacg ggttgctgca gcccagcggg
     3781 ctttctgatg ataccgtctc tcccgtgagg tagtcaaagg cctgcaggca gatcgtataa
     3841 ttatcttccg gaagcccctg accatgaatt agttctgaaa gtgtgacacc ctggcaaacc
     3901 aggtgctctt catcgaacac ctgctcgagg ttgaaacggt tgaggttgaa cggtaccccg
     3961 ggcaacaggg taaccggggg gtacatttta tatcccggat cggtatagac cctgatgccg
     4021 ttctccccct caaaaacacc gagaatataa acatctacag gcgccggggc attgcttaaa
     4081 aatgttgcca tcaccttgtc gggctgggag atgta
//
LOCUS       DAOQXS010000268         4108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22212_length_4108_cov_3.911424, whole genome shotgun sequence.
ACCESSION   DAOQXS010000268 DAOQXS010000000
VERSION     DAOQXS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22212_length_4108_cov_3.911424"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1238)
                     /locus_tag="PKM34_07985"
     CDS             complement(60..1238)
                     /locus_tag="PKM34_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNQ83567.1"
                     /translation="MTGENKHMKGDRVIWMVVILLSLLSLLAVYSSTGTLAYKYQEGN
                     TIYYMVKHMIILALGFGLMYLAHKVKYTYYSRIFQVALYVAILLLIVTLIFGKDVNEA
                     KRALQLPFGLTFQAADLAKLTLIIYLARELTKKQEVIHEFKSLFIPVMLPILVVCGLI
                     LPGNLSTAVMLFVTSLVLLFIGRIRILNLMKLVGIAAAAFLLFIGILMLLPQGSQGRL
                     KTWENRMESFLAGADQEEDDQVVQSKIAVVSGGIFGKMPGNSTQRNFLSHPYSDFIFA
                     IILEEYGLLGGSFVVLLYMILLFRAVRIVTKTPRTFGAFLTIGVAFSLVFQALINMGV
                     AVDLLPVTGQPLPLVSMGGTSIWFTSLSIGIILSVSKEVGKGTVENEPKEPEEQYVTA
                     "
     gene            complement(1246..2589)
                     /gene="murD"
                     /locus_tag="PKM34_07990"
     CDS             complement(1246..2589)
                     /gene="murD"
                     /locus_tag="PKM34_07990"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ83568.1"
                     /translation="MNRHDIIILGGGESGAGAAVLARLKGYHPMVSDNGIIKEKHKKL
                     LTDHGIEFEENKHSEATILDADEIIKSPGIPDKAPIIQSITAKGIPVISEIEFASRFT
                     DARLIFITGSNGKTTTTLLTHYILTKGGLNAGLAGNVGKSFALQVATEQHDIYVLEIS
                     SFQLDGMFKAKADIAVLMNITPDHLDRYNYNFQEYIDSKFRVINNQGHGDHFIFSFDD
                     PVILEHLSKKEVPSRHFPFTSGDKAFREGAFVRDNQVVFNINEKETSMTLEELALQGR
                     HNLYNSMAAGISARLLEIRKETIKQCLSDFQHIEHRLEFVASIHGVDYINDSKATNVN
                     STWYALESMHKTVVWIAGGLDKGNDYASLIPLVTAKVRVIICLGKDNRKIIETFGDLV
                     ETVIEVQTADDAVRAAYSLAHRGEAVLLSPACASFDLFENFEDRGNQFKKAIKNL"
     gene            complement(2592..3833)
                     /gene="mraY"
                     /locus_tag="PKM34_07995"
     CDS             complement(2592..3833)
                     /gene="mraY"
                     /locus_tag="PKM34_07995"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNQ83569.1"
                     /translation="MLYYLADYLQRCCDVPGAGVFQYISFRTAAAVIVSLLISLVFGT
                     KWIRYLNKRQVGETIRDLGLEGQLAKQGTPTMGGLIILASILIPTILFAKLDNIYITL
                     MLLTTVWLGSVGFLDDYIKVFKKNKNGLRGKYKILAQVILGIAVGATMFFSDGVVIRE
                     KVTQTSPSTEEITTSTGVSEKFSAVSVKSTKTTIPFVKNNEFDYAVLLRWLGTGYEKY
                     AFLIFIPLVIFIITAVSNGANLTDGMDGLATGTSAIIGATLGLLAWVSGNIIFADYLN
                     IMYLPNTGELTIFIGAFVGGCIGFLWYNSYPAQVFMGDTGSLALGGIIAVFAIIIRKK
                     LLIPILCGIFVMESLSVVMQVAYFKFTRKRFGEGRRIFKMSPLHHHFQVLGYPEPKIV
                     QRFMIVGIILAVITVITLKLR"
     gene            complement(3836..>4108)
                     /locus_tag="PKM34_08000"
     CDS             complement(3836..>4108)
                     /locus_tag="PKM34_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNQ83570.1"
                     /translation="TSDNPRLEEPEEIIAEMQAGISAELKRKVMVIVNRREAIRTACH
                     LAKPGDIILVAGKGHEKYQEIKGIRHHFDDKEILEEMLLNENQKPI"
BASE COUNT          985 a         1052 c         1020 g         1051 t
ORIGIN      
        1 tggctattgc cgggaaaata tgccccccgg tacctccgcc gctgataatg atatgtggat
       61 caggccgtga catattgctc ctccggttct tttggttcgt tttcaacggt ccctttgccc
      121 acttctttgc tgacgctcag gatgataccg atcgacaggc tggtgaacca gatcgaagtt
      181 ccgcccatgc tcaccagcgg aagtggctga ccggtcaccg gcagtaaatc gacagcgacg
      241 cccatgttga tcagcgcctg gaaaaccagg ctgaacgcca ccccgatggt caggaaggcg
      301 ccgaatgtcc ggggcgtctt ggtcacgatc ctcacggctc tgaaaagcag gatcatgtaa
      361 agcagcacga cgaaggagcc gcccagcagg ccatactcct cgaggatgat ggcaaagatg
      421 aaatcggagt aagggtggga gagaaagttc cgctgggtgc tgttacccgg catttttccg
      481 aatattccgc ccgaaacaac cgctattttt gactgaacga cctggtcgtc ttcttcctgg
      541 tcagcaccgg ctaaaaagct ctccatacgg ttctcccacg ttttcagacg gccctggctc
      601 ccctgtggaa gcaacatcag tatgccgatg aaaagtaaaa aagctgccgc tgcaatcccc
      661 accagtttca tcaggttcag gattcggatg cggccgatga atagcagcac caggcttgtc
      721 acgaacaaca tgaccgctgt ggagagatta cccggcagga tcagtccgca aaccaccagg
      781 ataggtagca tgaccggaat aaacaacgat ttgaattcat ggatgacctc ttgtttcttt
      841 gtcagttccc tggccaggta aataataaga gtaagcttgg ccaggtctgc cgcctggaag
      901 gtcaggccga agggtaactg caaagctctt ttggcttcat ttacgtcttt tccgaatatc
      961 aatgtcacta tcaatagaag gatggctacg taaagggcga cctggaaaat ccgtgagtag
     1021 taggtgtatt tcaccttgtg cgccaggtac atcaggccaa agcccagcgc caggatgatc
     1081 atgtgcttga ccatatagta gatcgtgttt ccttcctggt atttgtatgc cagcgttcct
     1141 gtcgagctgt aaacagccag cagggaaagc agcgatagca ggatgactac catccagatc
     1201 acacggtcgc ctttcatatg tttgttctcg ccggtcatct caggatcaga ggtttttgat
     1261 ggcttttttg aactggttgc cgcggtcctc gaaattttcg aacaggtcga aactggcgca
     1321 agccggcgac agcaatacgg cttcaccccg gtgggccagg ctgtaggctg ctcttaccgc
     1381 atcgtcggcc gtttgcactt cgataacggt ttccaccaga tcaccaaagg tttcgatgat
     1441 cttccgattg tctttgccca ggcagataat caccctgacc ttggccgtaa cgagcgggat
     1501 cagtgatgcg taatcgttcc ccttgtcgag cccgccggct atccatacaa ccgttttgtg
     1561 catgctttcg agcgcatacc aggtcgaatt gacattcgtg gctttggaat cattgatgta
     1621 atcaacacca tggatactgg ccacaaattc aagcctgtgc tctatgtgct ggaaatcgga
     1681 aagacactgc tttattgtct ccttgcggat ttccagcagc cgggcggaaa tacctgccgc
     1741 catcgaattg tacaggttat gcctgccctg taaggctaat tcttctagag tcatagaagt
     1801 ttctttttca ttaatgttga aaacaacttg attatccctg acaaaagctc cttctcggaa
     1861 agccttgtcg ccagatgtga atggaaaatg cctggatggg acctcttttt tgctcagatg
     1921 ttccaggatg accggatcat cgaaactgaa aatgaaatgg tcaccgtgtc cctggttgtt
     1981 gataacccgg aatttagaat cgatatattc ctggaaattg taattgtaac ggtccaggtg
     2041 atcgggtgta atgttcatca gaactgcgat atctgccttc gccttgaaca tgccgtcgag
     2101 ctgaaaactg ctgatctcca ggacatagat atcgtgctgt tcggtggcta cctgcaatgc
     2161 gaagctctta ccgacattgc ctgccagtcc cgcattcaga ccgccttttg tgagaatata
     2221 atgcgtgaga agggtagtgg tggtcttgcc gttgctcccc gtgatgaaga tcaggcgggc
     2281 atcggtgaag cgggaggcaa actcgatctc tgaaatgacc ggtatccctt ttgccgtaat
     2341 ggattgtatg ataggcgcct tatctggaat ccccgggctt ttgatgattt catccgcatc
     2401 caggattgtc gcttcggaat gtttgttttc ctcaaattca atcccgtgat cagttaaaag
     2461 cttcttatgt ttttctttta tgataccatt atcggatacc attggatgat agcctttcag
     2521 ccgtgccagg actgccgccc cggcgccgct ttcgcctcct cccagtataa tgatatcgtg
     2581 acggttcatc tttatctcag ttttaatgtg atgacggtga tgacggccag gatgatcccg
     2641 acgatcatga accgttggac aatctttggt tccggatagc ccagcacctg gaaatgatga
     2701 tgcaggggcg acattttaaa aatcctgcga ccttctccga acctcttccg tgtgaatttg
     2761 aaataggcaa cctgcatgac gaccgaaagg ctttccatca cgaatatccc gcaaaggatg
     2821 gggatcagga gcttcttacg gatgataatg gcaaaaacgg caatgatccc gcccagggcc
     2881 agactccccg tatcgcccat gaaaacctgc gccggatagg aattgtacca gagaaatccg
     2941 atacagcctc cgacgaaggc cccgatgaat atggtcaact cgccggtatt gggcaggtac
     3001 atgatattca ggtaatcagc aaagatgatg ttgcccgata cccaggccag cagcccaaga
     3061 gtggccccga tgatggccga ggtgccggta gccagcccgt ccatgccgtc ggtaagattg
     3121 gcgccgttgg atactgccgt aatgatgaaa atcaccagcg gtataaaaat caggaacgca
     3181 tacttttcgt agcctgtgcc taaccaccta agcaatacgg cataatcaaa ttcattgttc
     3241 ttgacgaatg gaatggttgt tttggtcgat ttgaccgata ccgcgctgaa tttctccgaa
     3301 acgccggtgg aggtagtgat ctcttcggtt gaagggctgg tctgtgtaac tttttcccgg
     3361 atgacaacgc catcgctgaa gaacatcgtg gcaccgacag caatgccaag aatgacctga
     3421 gccagtattt tatattttcc ccttaaacca ttcttgtttt ttttgaaaac tttaatgtaa
     3481 tcgtccagaa agccgaccga gccaagccat acagtcgtta gcaacatcag ggtaatgtaa
     3541 atgttgtcga gtttggcgaa caggatagtc gggatgagga tggaagccag gatgatgagc
     3601 ccgcccatgg tcggggttcc ctgcttggcc agttgcccct ccaggcccag gtcgcggatc
     3661 gtttccccga cctgcctttt gttcaggtat ctaatccatt tcgttccaaa aaccagcgag
     3721 ataagcaaag aaacgatcac tgccgccgcc gtacggaagg agatgtactg gaaaaccccg
     3781 gctcccggca catcgcaaca tctttgcaaa taatcggcta agtagtacag catgattata
     3841 tgggtttttg gttttcgttc agtaacatct cttccagaat ctccttatca tcgaaatgat
     3901 gccggatacc ttttatttcc tggtattttt catggccctt tccggcaacc aggataatat
     3961 cgccgggctt tgccagatgg caggccgtcc ggatcgcttc cctccggttg acgatcacca
     4021 tcactttccg cttcaactcg gcggaaatgc ccgcctgcat ttcggcaatg atctcttccg
     4081 gctcctccaa ccgcggattg tctgaagt
//
LOCUS       DAOQXS010000269         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22314_length_4096_cov_3.719624, whole genome shotgun sequence.
ACCESSION   DAOQXS010000269 DAOQXS010000000
VERSION     DAOQXS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22314_length_4096_cov_3.719624"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PKM34_08005"
     CDS             complement(<1..1430)
                     /locus_tag="PKM34_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ83571.1"
                     /translation="MHKGRLKRLRVILSLLFFAVLALLFLDFTGRIYGKASHLFLHLQ
                     FVPSLVQFAGTLSLAALGFAFIIILTLLFGRIYCSSFCPLGTLQDIVIYLSKKIKRKR
                     RIFKFKKPSYRLHYGILALVVLSLLTGSILLVNLLDPYSAFGKIFANLFRPLVVAVNN
                     LISIGLSYADIYSVPRIKWVPVNIAALFLSLAWLILVVYLSVRRGRIYCNTVCPVGAL
                     LGLFARFSLFRIVLDKTKCNSCGKCSAVCKAECINPKDQSVDFSRCVGCMNCLSPCPD
                     KGVKIRPFWVKDTVQVFEFDAGKRDFFKKSGLVVVGVFAGSKVFSEEKEKEVIKATVP
                     VVRQFPVTPPGAESQERFNKLCTACHLCVSACPTHVLQPTYFLYGMTGFLQPRMDYIT
                     SFCNFDCVLCGEVCPTGAILNITTEEKKRIQLGKSYFIKENCVVYTHNTACGACSEHC
                     PTKAVQMVPYKDKLTIPEVNNTICVG"
     gene            complement(1433..2371)
                     /locus_tag="PKM34_08010"
     CDS             complement(1433..2371)
                     /locus_tag="PKM34_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ83572.1"
                     /translation="MKRRDFIRKSAAAAAFTGAAISLPFGSKLFSSPSHPYNPSNPSP
                     FDLVAIKGGEVEAMFDQAIAAMGGMQAFVKKGQKVVVKPNIGWDVSPERAGNTNPALV
                     KRIVRHCLDAGAKEVYVFDNTCDKWDRCYATSGIEQAVKDAGGKLAPGNTENYYQEVQ
                     IPNGKKLKSTKVHELILDSDVFINVPVLKSHGSARLTISMKNLMGVVWDRGWWHRNDL
                     NQCIADYATYLKKPTLNIIDAYRVMMKNGPRGVSEADVSLMKSLIISPDMVAADAAAT
                     KMFGISPDDVPYIGIADSMGIGSMALDKLNVHRIIL"
     gene            2531..3655
                     /gene="hemW"
                     /locus_tag="PKM34_08015"
     CDS             2531..3655
                     /gene="hemW"
                     /locus_tag="PKM34_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNQ83573.1"
                     /translation="MAGIYIHIPFCKQKCHYCNFFSVASVKWRDEFTRALLKEMELRK
                     DYLQGEPVNTVYFGGGTPSLWSIAHLALCIAHLALHFNIEHGVEITLEANPDDINETS
                     LEEWRKAGINRLSIGIQSFFDDDLRYLNRVHDAGQAHRAIEAARRVGFDNLTIDLIYG
                     IPGLTEEKWAKNLETFFSLDIPHLSAYALTVEQKTPLALLIEKGKYAPVDEELSVRHF
                     KMLLEQAKANDFIHYEISNFAREGYYSKHNSLYWLGGHYLGLGPSAHSFNGHSRQWNV
                     SNISQYIKLDEYQSSVDEKEVLSTDQRYNEYVMTSLRTVWGCDTVHIRNVFGEPFENH
                     FIKKSHLFIKKNHLYREGTKYFLTEEGKLFADGIAAGMFR"
     gene            3701..>4096
                     /locus_tag="PKM34_08020"
     CDS             3701..>4096
                     /locus_tag="PKM34_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNQ83574.1"
                     /translation="MPIIGQLVKRAYELGRLPDIAKPKLDPYTAQNRVLKKLLRRAQF
                     TAFGEYYGFSDILKSPDPIKAFQAKVPTFTYNTMFRKWWYRALNGEGFVSWPGRVKYF
                     AVTSGTSEASSKHIPVTSEMIRAIRKVSIR"
BASE COUNT         1027 a         1005 c         1095 g          969 t
ORIGIN      
        1 catccaacgc aaatggtgtt gttgacttcg gggatggtga gtttgtcttt gtatggaacc
       61 atctggacgg ctttggtcgg gcagtgctcc gagcaggctc cgcaggcggt gttatgcgtg
      121 tagaccacgc agttctcctt tataaaatag gatttgccca gctgtatccg ctttttctcc
      181 tccgtggtga tattcagtat ggcgccggtg gggcagactt caccgcataa aacacaatca
      241 aaattgcaga agctggttat atagtccatc ctgggctgga ggaacccggt catcccgtaa
      301 aggaaatagg tgggctgaag gacatgcgtc gggcaggctg aaacacagag atgacaggct
      361 gtacagagct tgttgaaccg ctcctggctc tctgctccgg gaggggtaac gggaaactgc
      421 ctgacaacag gaacggtggc ctttatgacc tctttttcct tttcctcaga aaatactttg
      481 ctcccggcaa aaacgccaac gacgaccaat cccgattttt taaagaaatc acgctttcct
      541 gcatcaaatt caaacacctg cacggtgtct ttaacccaga aaggtctgat tttcacccct
      601 ttatccggac agggggagag gcagttcatg caccccacac accggctgaa atcgaccgac
      661 tggtctttcg ggttgataca ttccgccttg cataccgccg aacacttgcc gcaactgttg
      721 catttggtct tatcgagcac gatcctgaaa agcgaaaatc ttgcaaacaa gcccagcagc
      781 gccccgaccg ggcaaacggt attgcaatag atccttcctc ttcttaccga aagatagacc
      841 acaaggatca gccaggccag cgagaggaac agggctgcga tgttcaccgg cacccatttg
      901 attctcggaa cagaatagat atcggcataa gataagccaa tggatatcaa attattgaca
      961 gcaacgacca ggggccggaa aaggttggcg aagattttac cgaaagcgct gtagggatcg
     1021 agcaggttga ccagcaggat gcttcccgtg agaagcgaaa gcacgaccag ggccaggata
     1081 ccgtaatgaa gccggtatga aggtttctta aatttgaaga ttcttctttt tcgtttgatt
     1141 ttttttgaaa gatagataac gatatcctgc aaggtgccga gcgggcaaaa ggaggagcag
     1201 taaatccttc cgaaaagaag cgtcaggatg atgataaagg cgaatccaag tgcggccaga
     1261 gagagggtac cggcaaactg caccagtgaa gggacgaatt gcaggtgtaa aaacaggtga
     1321 gacgccttgc cgtaaattct cccggtaaaa tcgaggaaca ggagggcgag gaccgcaaaa
     1381 aacaggagcg acaggatgac acggagtcgt ttcagccggc ctttgtgcat gatcagagga
     1441 taatgcggtg gacgttcagc ttatcgagcg ccatagaccc gatgcccatg gaatcggcga
     1501 tcccgatgta ggggacgtca tctggggaga tgccgaacat tttggttgct gcagcatcgg
     1561 ctgccaccat atcgggcgag atgatcagcg atttcatcag gctgacgtcg gcttcggaga
     1621 cgccgcgcgg accgttcttc atcatgacgc ggtaggcgtc gataatattg agggtcggtt
     1681 tctttagata tgtcgcataa tcggcaatgc actggttcag gtcgttccgg tgccaccagc
     1741 cgcggtccca caccactccc atcaggtttt tcatagagat ggtcagccgg gccgaaccat
     1801 ggcttttcag gacgggcaca ttgataaaga catcggagtc gaggataagt tcatgaactt
     1861 tggttgattt cagttttttg ccatttggaa tctgcacttc ctggtagtag ttttctgtgt
     1921 tcccgggggc cagtttgccg ccggcatctt tcacggcctg ttcaatgcca ctggtggcat
     1981 agcagcggtc ccatttgtcg caggtgttat cgaaaacata gacctctttg gcgccagcat
     2041 cgaggcaatg ccttacgatc cttttcacca gggccggatt ggtattgccg gctctttcgg
     2101 gcgaaacatc ccagccgata ttcggtttaa cgacaacttt ctgacccttt ttaacaaacg
     2161 cctgcatgcc gcccatcgcc gcgatggcct ggtcgaacat ggcttccact tcaccgcctt
     2221 tgatggcgac gagatcgaaa ggggaggggt tggaagggtt gtaagggtgg gagggggagc
     2281 tgaagagttt ggagccgaag ggaagggaga tggcggcgcc ggtgaatgct gctgctgctg
     2341 cgcttttgcg tatgaaatca cgtcttttca tgatcgattt gattttttcg tatgctaaag
     2401 tacatatatt tgtcattatt gctgaaataa aaatccgtgt ttatccgcca ggatccgtgt
     2461 catccgcgtt ctatcaacag cacgcggata ccgcggatga ggcggatgta cgcggataat
     2521 tcgctttcaa atggctggaa tctatatcca tatacctttt tgcaaacaga aatgccacta
     2581 ctgcaatttc ttctccgtcg cttcggtaaa atggcgtgac gagtttaccc gggcgctatt
     2641 aaaagagatg gagctccgga aggactacct gcagggtgaa ccggtgaaca cggtctattt
     2701 cgggggagga accccgtccc tttggtccat tgcgcattta gcattgtgca ttgcgcattt
     2761 ggcattgcat tttaacattg aacatggggt ggaaattaca ctggaggcga atcctgatga
     2821 tattaacgaa acatcgctgg aagagtggag aaaggccggt atcaaccgtt tgagcatcgg
     2881 gatccagtcg tttttcgacg atgatctgcg ctacctgaac cgggtgcacg acgccggcca
     2941 ggcgcaccgg gccatcgagg cagcccggcg tgtgggtttc gataacctga ccatcgacct
     3001 gatttacggc attcccgggt tgacggagga gaaatgggca aagaacctcg aaaccttctt
     3061 ctcactcgat attccgcacc tttcggccta tgccctcacg gtggaacaga aaacgccgct
     3121 ggccctgctt atcgaaaaag gaaaatacgc gccggtcgat gaggagctgt cggtcaggca
     3181 tttcaaaatg ctgctggaac aggcaaaagc caatgatttc atccattacg agatatccaa
     3241 ttttgccagg gaagggtatt attcgaagca caacagcctt tactggctgg gcgggcacta
     3301 cctcggcctg ggcccttccg cccattcgtt caacggccac tcgcggcagt ggaacgtttc
     3361 caacatcagt caatacatca aactggatga atatcagtca tctgtggatg aaaaagaagt
     3421 gctttccacc gaccagcgct acaacgaata tgtgatgacc tcgctgcgga cggtatgggg
     3481 ctgcgacact gttcacatcc ggaatgtttt cggggagccg tttgaaaatc atttcataaa
     3541 aaaatcgcat ctgtttatca aaaaaaatca tctttaccgg gaagggacga agtatttcct
     3601 tacggaagaa gggaagctgt ttgccgatgg gatcgcggcg gggatgttca gatgataaca
     3661 ttaattctta cttttgtctg accattttga aattcaccag atgccgataa ttggacaact
     3721 tgtcaaacgg gcctatgaac ttggccgact gccggatata gccaaaccca aactcgatcc
     3781 gtacacggcg cagaacaggg ttcttaaaaa actcctccgg cgcgcccagt tcactgcttt
     3841 cggagaatac tacggttttt ccgacatcct taaaagcccc gatccgatca aagccttcca
     3901 ggcaaaggtg ccgacattca cctacaacac gatgttccgc aaatggtggt acagggcgct
     3961 caacggcgaa ggcttcgtga gctggcccgg gcgggtgaaa tatttcgctg tcacatcagg
     4021 cacttcggaa gcctccagca agcatattcc cgtcacttcc gaaatgatcc gggcgatccg
     4081 gaaagtcagc atccgg
//
LOCUS       DAOQXS010000270         4095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22319_length_4095_cov_3.000743, whole genome shotgun sequence.
ACCESSION   DAOQXS010000270 DAOQXS010000000
VERSION     DAOQXS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22319_length_4095_cov_3.000743"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PKM34_08025"
     CDS             <1..975
                     /locus_tag="PKM34_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ83575.1"
                     /translation="NKISEESHVFADKFEFAGGYGTSLLEEVSRYALVASALATSKDF
                     DLIHSHDWLTYPAGIAAKYVSGKPLVIHVHATEFDRSGENVNPPVFEIERKGMEEADK
                     IIAVSNFTRNIIIEKYGIPEEKVVTVHNAVEPAEIKEINGAKYVPEKVVTFLGRITFQ
                     KGPDYFIEAARLVLERDPNVRFVMAGSGDLLEKMIRRVAELRMGDKFHFTGFLKGTAV
                     DRMLAMSDVFVMPSVSEPFGIVPLEAMRSNVPVVISKQSGVSEVLKHALKVDFWDVHG
                     MADAIYSVLHYNGLNRMFTKYGKDEVESLKWEHAGLKVKDVYEEMLKK"
     gene            996..2354
                     /locus_tag="PKM34_08030"
     CDS             996..2354
                     /locus_tag="PKM34_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNQ83576.1"
                     /translation="MRSVCFYFQIHQPVRLRTYRFFDIGAYHNYYDDYQNRFILRRVA
                     DKSYLPMNKLLLELIKEYGPAFRVSFSISGLALEQMKMYAPDVLQSFKKLADTGSVEF
                     LAETYAHSLSSLRSRDEFISQVQKHREMIKELFGVEPVSFRNTELIYSDGIGEMAAEM
                     GFKAALTEGARHILGWKTPNFIYCNALNPKLKVLMRNYQLSDDIAFRFSERGWSEWPL
                     TTEKFVDWLNAFDRKHEVVNLFMDYETFGEHQWRETGIFDFMKALPARIFSHSDFTFN
                     TPSELADKLQPVSAIHVEYPISWADEERDLTAWLGNDLQDDAFDNLYALEPLVKQSDD
                     PEIRKDWCFLQASDHFYYMCTKWFSDGDVHKYFNPYGSPYDAYINYMNVLADFRLRLE
                     KRGSLYATRHAPANEPDAVVPAPVKKAKKDAKDIKPVTKTKQKTAKKPVLKKKDAPKK
                     PR"
     gene            2447..>4095
                     /locus_tag="PKM34_08035"
     CDS             2447..>4095
                     /locus_tag="PKM34_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNQ83577.1"
                     /translation="MEHPNKLKPDYVFEVSWEVCNKVGGIHTVISTKAPFMCKEYGEH
                     YIVLGPDVWKETLANPEFTEDIQLFRAWKEKAQSDGLNIRIGHWNIPCRPIAILVDFT
                     TFFSRKDQILAEFWETYQLDSISGGWDYIEPVMFGYGAAKVIENFYEFHFTSRERIIA
                     HFHEWMTGAGVLYLEKYVPQAGTVFTTHATMLGRAIAGNGKLLYENMEKLDAGALAHS
                     YQVTSKYSLERCASNVADCFTTVSEITSRECKLLLGKETDFVTPNGFDPGIIPGPGQM
                     AEKRAAARETLLEVTRKLTCTDIPDDSVMLLISGRYEFRNKGIDLFIESLGELNQLEG
                     APILACIMVPANVAGPNRALLEDCHETPSQKYLTHFIHHEEYDPVLHKIRESGLSNNP
                     DDRVKIIFIPAYMNGNDGIFNLHYYDLLAGFDLTVFPSYYEPWGYTPLESAAFGIPTV
                     TTTLAGFGLWIKSVYRKKNEAVKVLTRTDTNTAELLTELIRHIQAFTLKNPEEKESLK
                     SLAAGLSKTSLWDEFFGYYREAYSFALSKSDERFELFRDKR"
BASE COUNT         1095 a          995 c         1026 g          979 t
ORIGIN      
        1 aacaaaattt cggaagagag ccacgttttt gctgataaat tcgagtttgc aggaggatac
       61 ggcacgagcc ttctggaaga agtctcccgc tacgccctcg tggcctctgc tctcgcaaca
      121 tcaaaagatt tcgatctgat ccattcgcac gactggctga cttacccggc cggcatcgcc
      181 gcaaaatatg tcagcggcaa accgctggtc atccatgtcc atgctaccga attcgaccgt
      241 tccggcgaaa atgtgaatcc gcccgttttt gaaatcgaga ggaaagggat ggaagaggcc
      301 gataagatca tcgcggtaag caattttaca aggaacatca tcatcgagaa atacggcatc
      361 ccggaagaaa aagtggttac cgttcacaat gcggtggaac cggctgagat aaaagagatc
      421 aacggggcca aatatgtccc ggaaaaggtc gtgaccttcc tgggccggat cacattccag
      481 aaaggccccg attatttcat tgaagccgcc aggctggtac tggagcgcga tcccaatgtg
      541 aggtttgtta tggccggcag cggcgatctg ctcgaaaaaa tgatccgccg cgtggccgaa
      601 ctccggatgg gcgacaaatt ccacttcaca ggatttctga aagggacagc ggtcgaccgg
      661 atgctggcca tgtccgacgt cttcgtcatg ccgtcggttt cggagccttt cggtatcgtt
      721 ccgctggaag cgatgcgctc caatgtgccc gtggttattt caaagcagtc gggtgtatcg
      781 gaagtgctga aacatgccct taaagtcgat ttctgggatg tgcacggcat ggccgacgcc
      841 atctattcgg tccttcatta taatggcctt aaccggatgt ttaccaaata cggcaaagac
      901 gaggtggaaa gcctgaagtg ggaacatgcc ggcctgaagg tcaaagacgt ttacgaagaa
      961 atgttaaaga aataataaat ccaaaacagc acgaaatgcg ctcagtttgc ttttatttcc
     1021 agatccatca gcccgtgagg ctccggactt accggttttt cgatatcggc gcttatcata
     1081 attattatga tgattaccag aacaggttta ttctcaggag agtagcggat aaatcctacc
     1141 tgcccatgaa taaattgctg ctggaactga tcaaagaata cggtccggct ttccgggtga
     1201 gcttttctat ttccgggtta gcgctggaac agatgaaaat gtatgcaccc gatgtactgc
     1261 agagttttaa aaaactagcc gacacaggca gtgtggagtt tctggccgag acatacgctc
     1321 attcgctgag ctcgctgcga agcagggatg aattcatcag ccaggtacaa aagcaccggg
     1381 aaatgatcaa agaactcttc ggcgttgagc ctgtatcatt cagaaatacc gagcttatct
     1441 attccgacgg tatcggtgaa atggcggcgg aaatgggatt caaagcggcc ctcaccgaag
     1501 gggcccggca tatcctcgga tggaaaacac cgaactttat ctattgtaat gctcttaatc
     1561 ctaaactgaa ggtgcttatg cggaattacc agctatcgga tgatatcgcc ttccgttttt
     1621 cggaaagggg atggtcggaa tggccgctga caaccgagaa gttcgtcgac tggctgaatg
     1681 ctttcgaccg taagcatgag gtagtgaacc tcttcatgga ttatgagaca ttcggtgaac
     1741 accagtggag ggaaaccggt atcttcgatt ttatgaaagc attgccggcc agaatcttct
     1801 cgcattctga ctttacattc aatacaccgt cagaactggc agacaaattg cagcccgtat
     1861 cggccatcca tgtcgaatac ccgatctcat gggcggatga ggaacgggac ctgacggcct
     1921 ggctgggcaa tgaccttcag gatgacgcct ttgataatct ctacgctctc gaacctttgg
     1981 tgaaacagtc ggacgatccc gaaatcagaa aggactggtg ctttctgcag gcatccgatc
     2041 acttctatta catgtgcaca aaatggttct ccgacggcga tgtccataaa tatttcaacc
     2101 cttacggatc accgtatgat gcgtatatca actatatgaa tgtcctggcc gatttcaggt
     2161 taaggcttga aaaaagaggg agtctatatg ccacccggca tgctccggcg aatgagccgg
     2221 atgctgtggt gccggccccg gtgaagaagg ctaaaaaaga tgctaaagac atcaaaccgg
     2281 ttaccaaaac taaacagaaa acagctaaaa aacctgtgct caaaaagaaa gatgctccga
     2341 aaaagccgcg ctaatctgat tttttctgca ttatttcctg tttctttttc cgaaccttct
     2401 tttttgattt aaaaagatat ttttgcacag catttaatta cttttcatgg aacaccccaa
     2461 taaactaaaa cccgactatg tatttgaagt cagctgggaa gtctgtaata aagtcggtgg
     2521 aattcacacg gttataagca ccaaggcgcc gttcatgtgc aaagaatacg gcgagcacta
     2581 tattgtcctg ggtccggatg tgtggaaaga aaccctggcc aatcctgaat ttacggaaga
     2641 catacaactg ttccgtgcct ggaaggagaa agcacagtca gacggactca atatccggat
     2701 cggacactgg aacattccct gccgcccgat tgccatcctg gtcgatttta caaccttctt
     2761 ttcccggaaa gaccagatac tggcagagtt ctgggaaacc taccagctcg actcgatctc
     2821 cggcggatgg gattacattg agccggtcat gttcggctat ggcgcggcaa aagtgatcga
     2881 gaacttttac gaattccatt tcacgtcgag ggaaaggatc attgcccatt ttcacgaatg
     2941 gatgaccggc gcaggcgttc tctatctgga aaaatatgtc ccccaggccg gtacggtctt
     3001 tacgacacac gcgactatgc tcggaagggc gatcgccggt aatggaaagc tgctatatga
     3061 aaacatggaa aagctcgatg ccggtgcatt ggctcattcc taccaggtta catcgaagta
     3121 ttcactcgag agatgtgcat ctaatgtggc cgattgcttt acgaccgtat cggaaattac
     3181 cagccgggag tgtaaattgt tactgggcaa ggaaaccgat tttgtgaccc ccaacgggtt
     3241 cgacccgggg atcattcccg gacccggcca gatggctgaa aaacgggccg cagcccgcga
     3301 aacgttgctg gaggtaaccc ggaagctgac ctgcaccgat attcctgacg attctgtgat
     3361 gctgctcatc agcggacgct atgagttcag gaacaaggga attgaccttt tcatcgaaag
     3421 tttgggagag ctgaatcaat tggagggtgc gcctatcctt gcctgcatca tggtcccggc
     3481 taatgtggca ggcccaaacc gcgcactgct ggaggattgc catgagactc catctcaaaa
     3541 atacctgacc catttcattc accatgagga atacgatccg gtcttgcata agatcaggga
     3601 gtcggggctg tcaaacaacc cggacgacag ggtgaagatc atctttattc cggcctacat
     3661 gaacggtaat gatggtattt ttaacctgca ttattacgat ctcctggcag gtttcgatct
     3721 tacggtcttt ccttcttatt atgagccctg gggatatacc ccgcttgaaa gcgccgcatt
     3781 tggtatcccc accgttacca cgaccctggc cggcttcggg ctctggatca aatcggtcta
     3841 ccggaaaaag aacgaagccg tcaaagtact tacccgtacc gacacgaata cggcagaatt
     3901 gctgaccgag ctgatccgcc atatccaggc ctttacactg aaaaacccgg aagaaaagga
     3961 atcgctgaaa tccctggccg caggcctctc aaaaacttct ctttgggatg aatttttcgg
     4021 ttattacagg gaagcctatt cattcgcgtt atcgaagtcg gatgagcggt tcgaactgtt
     4081 ccgcgacaaa agaca
//
LOCUS       DAOQXS010000271         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22458_length_4078_cov_3.867263, whole genome shotgun sequence.
ACCESSION   DAOQXS010000271 DAOQXS010000000
VERSION     DAOQXS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22458_length_4078_cov_3.867263"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2967)
                     /locus_tag="PKM34_08040"
     CDS             complement(<1..2967)
                     /locus_tag="PKM34_08040"
                     /inference="COORDINATES: protein motif:HMM:NF037155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNQ83578.1"
                     /translation="MALLVAMAGCNKSTRTTDPVQFAEYVSGFTSGVISKADPVRIKL
                     ATGNLKFETGKDLPAGIISFEPSVAGTVSMYSEDLLIFKPSGEWPAGKTIKAFLRLDK
                     LMDVPPQFSTFNFQFSIITPSYSIYPGNLVSIGQGQNKMKKMEGKLVSADVMKLEEVD
                     KLLKARADGKRYKVKWAEGSGRNEFEFVIDSLPRNEQAYQLELEWNGSPLGIDHKGEL
                     AVTIPSIFDFIYLGYSIGQGEEQYLDIVLSDPVDPDQNLDGLVYLREGDPVRLVADNN
                     IIRLYPMERLERSRTLIVEASVRNDIRTSLKERVVQTVMFEELKPQAEFIGQGVIMPD
                     PAGLFLPVRAVSLRALDIVVYKIFENNIPYYLQENNFSDNHLYSLKQYGRPVYAKTLM
                     LDEDKSIDLHRWNNFSLDLSPLMEKDMGAIYRVRISFRKEYSVYHCDNKAETDILEFI
                     LDGSVPDDQLEKYDQPGWYSDYEWPDEYDWDEQGNPCHNSYYIPERFAQKLIMASKIG
                     VTAKSADTKQWIIHTTDLISGEPLTDVSVGLYNFQNQPVGSATTGPDGKAEVTAESRP
                     FFLKATKGKQTTWLRLDDGTSLSLSQFDISGAELQKGLKGMIYGERGIWRPGDTLFLT
                     FILDDELKRIPSDYPVLMEVYNSRGQIVYSQTRSDPAGQFYTFKVATLPDAPTGNWRA
                     VVKAGGAMFEKSLKVETVKPNRLKIKLDFDKEILNAFSGNPLGTLEVKWLHGAVAPRV
                     RTTVDMSFRKIKTGFRGYEKYAFDNPSGYFWWSEERNVLDQEMNDQGKGSFSLELPKE
                     NNAPGMMEAVFLVRAFEKGGDFSTDVLTKTFSPFKRYVGIKVPNGGTYDEMLETDEDQ
                     QIDIACVDWMGNPLTVRNLEVKVYRVDWRWWWSAGEDDLAYYVGGNDANLVYRGSADA
                     VNGKGTFRFRIDYPDWGRYLILVKDNEGGHQAGKAVYIDWPSFRSRAGRANPAGATML
                     TFTADKERYEPGDKAVI"
     gene            3237..4013
                     /locus_tag="PKM34_08045"
     CDS             3237..4013
                     /locus_tag="PKM34_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83579.1"
                     /translation="MVKISSVTVTLNEELNIERCLGSVRDVVDEMIVVDSFSTDRTEE
                     ICLENGAVFSKHPFAGYIEQKSYAISLASHDYILLIDADEALSDEMRDSIRKVKENWT
                     HDGYSFNRLNSFCGKWIKHSGWYPDRKVRLFDRRKNTVKGRNPHEEFVMNAGATLKHL
                     KGDLLHYTYLTVEDQVRQIDRFTAIQAREAFARGKKASWFLILFGPCYKFFRHYFLRL
                     GFLDGFYGYLICKNMAHSTFLKHAKLKALWIREKGIGKNN"
BASE COUNT          990 a         1073 c          990 g         1025 t
ORIGIN      
        1 gataacagcc ttatcgcccg gttcatacct ctccttatcg gcggtaaacg tcagcatcgt
       61 tgcgcccgcc gggttggccc gtcctgccct gcttctgaaa gagggccaat cgatatacac
      121 ggctttcccc gcctggtggc ccccttcatt gtctttcacc aggatcaggt agcggcccca
      181 gtcgggataa tcgatccgga aacggaaggt ccctttgcca tttacggcat ccgcactccc
      241 cctgtaaacc agattggcat cgtttccacc cacataatag gccaggtcat cctccccggc
      301 actccaccac caccgccagt caacccggta cactttgacc tccaggttcc tgacggtcag
      361 aggattgccc atccagtcga cacaggcaat gtcaatctgc tgatcctcat ccgtctccag
      421 catctcatca tacgttcctc cgttcggaac cttaatcccg acataccttt taaacggaga
      481 aaaagttttg gtaagaacat ccgtactgaa atccccacct ttttcaaaag cccggacaag
      541 gaaaacggct tccatcatcc ccggcgcatt gttctcttta ggcaattcga ggctgaaaga
      601 accctttccc tggtcattca tctcctggtc gagcacattc ctctcctccg accaccagaa
      661 atagccggaa ggattatcaa aagcatattt ttcatatccc cggaaaccgg ttttgatttt
      721 ccggaaactc atatcgaccg ttgtccttac ccttggagcc acagcgccgt gcagccattt
      781 gacctccagc gttcccaacg ggttgccgct gaaagcgttc aatatctctt tgtcgaaatc
      841 cagcttgatc ttcagcctgt taggtttgac cgtctccacc ttgaggcttt tctcgaacat
      901 cgcaccgccg gctttcacaa cagcgcgcca gttgccggta ggggcatccg gaagggttgc
      961 aactttaaaa gtgtagaact ggccggcagg atccgaccgc gtctggctgt aaacgatctg
     1021 gccgcgagaa ttatacacct ccatcagaac gggataatct gacgggatcc gctttaattc
     1081 atcatccagg atgaaggtca gaaagagggt atcgccaggc cgccagatac ctctttcacc
     1141 gtatatcatc cctttgagcc ctttctgaag ctctgccccg ctgatatcga actggctgag
     1201 cgacagcgag gtgccgtcgt cgagccggag ccaggtggtt tgtttgcctt tggtagcttt
     1261 gagaaaaaac ggccggcttt cggcggtaac ctccgccttc ccatccgggc cggtagtggc
     1321 cgatcctacg ggctgattct ggaaattata caacccgacc gatacatccg tcaaaggctc
     1381 acccgatatc aggtccgtcg tatgaatgat ccattgtttc gtatcggccg atttagccgt
     1441 cacgccgatc tttgaagcca tgatcagctt ctgcgcgaac ctttcgggga tgtaatagga
     1501 gttgtggcac ggatttccct gctcgtccca atcatattcg tcgggccact cgtaatcgga
     1561 ataccagccc ggctggtcat acttttccag ctggtcatcg ggaacggatc cgtcaaggat
     1621 gaattccaag atatccgttt cggctttatt atcacaatga tatacggaat attctttcct
     1681 gaaggagatc ctgacacggt agatcgcgcc catgtctttc tccatcagcg gcgaaagatc
     1741 gagggaaaaa ttattccacc ggtgcaggtc gatggatttg tcttcatcca gcatcagcgt
     1801 tttggcataa accggcctgc catactgttt aaggctgtat aaatggttat ccgaaaagtt
     1861 attttcctgg agataataag ggatattgtt ttcgaagatc ttataaacga cgatatcgag
     1921 ggcgcggagg ctgacggcgc gaaccgggag gaagaggccg gcgggatcag gcatgatcac
     1981 cccctggccg atgaattcgg cctgtggctt taactcctca aacatcaccg tctgcacgac
     2041 tctttctttc aatgatgtcc ggatatcgtt cctgaccgag gcttcgacga tgagcgtgcg
     2101 gctcctttcc agccgctcca tcggatagag gcggataatg ttattatcgg ccaccagcct
     2161 taccggatca ccttcccgca ggtaaaccag cccgtcgagg ttctggtccg ggtcgaccgg
     2221 gtccgaaagg acgatgtcga gatactgctc ttcaccctgg ccgatggaat atccgaggta
     2281 aatgaaatcg aagatcgatg gaatggttac cgcaagctct cctttatgat cgatccccag
     2341 cggggatccg ttccattcca gttccagctg ataggcttgt tcattccggg gcaaactgtc
     2401 gatcacaaat tcgaactcat tgcggcccga tccctcggcc cacttcactt tataccgttt
     2461 gccgtcagct ctggctttca gcagtttgtc aacctcttcc agcttcatga catcggccga
     2521 taccagtttg ccttccatct ttttcatttt gttctgtccc tggccgatcg agaccaggtt
     2581 ccccgggtag atgctgtaag atggggtaat gattgaaaat tgaaagttga aagtcgaaaa
     2641 ttgggggggt acatccatca gtttatccaa tctcaggaag gctttaatgg ttttacccgc
     2701 cggccattcg ccggaaggct tgaaaatcag caaatcttca gaatacattg atacggtccc
     2761 agcgactgaa ggctcgaacg agataatacc cgccggtaga tctttcccgg tttcaaattt
     2821 caaatttccg gtcgcaagtt taatcctgac cgggtcagcc ttggagatca ccccggaagt
     2881 aaatcccgaa acgtattccg cgaactgaac cggatcggtg gtccttgtcg atttattgca
     2941 accggccatc gcgaccagca aagccataat aaaacaaaaa ggtttggttt tcataacatt
     3001 agcctgattt taattaagat ggctttatat gccaccaagg ctccaaaaca ctaaataaca
     3061 ccaaaaaaat caatttggtg atctttggtg tcttggtgtt ttagtggcaa taatgccgca
     3121 aagcactatt cataacattt cttacaacaa agctccaagg tacgaataaa tcaaaggcaa
     3181 agaataaaaa ttgcatcttt atgaataact ttggccctcc gcaaaaattt tgcattatgg
     3241 taaaaatttc ttccgtaacc gttacgctga acgaggagct gaacatcgaa cggtgcctgg
     3301 gatcggtccg tgatgtggtg gatgagatga ttgttgtcga ttccttttcg accgaccgga
     3361 cggaggagat ttgcctggag aacggagctg ttttcagcaa gcatcctttc gccggatata
     3421 ttgagcagaa gagctatgcc atttcgctgg caagccatga ttacatcctt ttgatcgatg
     3481 cagacgaagc gttaagcgat gagatgcggg actctatccg gaaggtgaaa gagaactgga
     3541 cgcacgacgg gtacagcttt aaccggctga acagcttttg cgggaaatgg ataaagcaca
     3601 gcggctggta tccggatcgc aaggtaaggt tgttcgaccg gcggaagaat acggtcaagg
     3661 gccggaatcc ccatgaagag tttgtgatga acgccggcgc caccttaaaa catctgaaag
     3721 gcgacttgtt gcattacacg tatcttacag tggaggacca ggtgcggcag atcgaccggt
     3781 tcacggccat acaggcccgg gaagcttttg ccagggggaa gaaagcgagc tggttcctga
     3841 tcctgttcgg cccctgttac aagttcttcc ggcattattt tctccggctc ggattcctcg
     3901 acggtttcta tggttatctc atctgcaaaa acatggcaca ttccacgttt ttgaagcacg
     3961 cgaagctgaa agcgctttgg atcagggaaa agggaatagg gaaaaataat tagattaggg
     4021 gaagtgggat gtactggatt cgaaccagtg acctcatgcg tgtgaagcat gcgctctg
//
LOCUS       DAOQXS010000272         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22509_length_4072_cov_3.083645, whole genome shotgun sequence.
ACCESSION   DAOQXS010000272 DAOQXS010000000
VERSION     DAOQXS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22509_length_4072_cov_3.083645"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..868)
                     /gene="cdaA"
                     /locus_tag="PKM34_08050"
     CDS             complement(35..868)
                     /gene="cdaA"
                     /locus_tag="PKM34_08050"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNQ83580.1"
                     /translation="MLAILLTGLFQIRLLDVLDILLVAFILYELYNLLKGSVSINMFF
                     AIVAMFFIWRVTDALQMEMLREILGAFFSVGIIALFIIFQPELRQFLLTLGKPDFIQK
                     RRRRFLFWHFNEASPYVVDVDKIVHACQKMANSKQGAIIIITRLHELKIIKDTGQYLN
                     AEISVELLENIFFKNSPLHDGAVIISGNKIEAARCILPVSKSQDLTAGLGLRHRAAVG
                     VTEQSDAIAIVVSEQTGKISYAESGELVRNVQPSALRDLLEEKLQAVPKERENAVTET
                     V"
     gene            complement(956..1912)
                     /gene="folP"
                     /locus_tag="PKM34_08055"
     CDS             complement(956..1912)
                     /gene="folP"
                     /locus_tag="PKM34_08055"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ83581.1"
                     /translation="MIKKVDKNTSFSETKVFEYNGKTMDFRVPRVMGIVNVTPDSFYD
                     WTVGRFGDLTIGRLDDLAVGRFGDLAVGRFGDLAVGRFGDLMGRVKEMADSGAYIIDI
                     GAVSTRPGAPEVSVEEEKSRLLPVLKEVRKSFPEIIISIDTYRSEIAEIAAGEGADMI
                     NDISGGMFDPLMLSCIIKLKIPYIIMHIQGTPATMQQNPTYADVVMEVKDFLLGQVRR
                     LESAGHHKIIIDPGFGFGKTVEHNYQILSHLDEFVDTGYPVLAGLSRKSMINKVLGTK
                     PAEALNGTTVLNTIALMKGASILRVHDVKEATETVKLFIKAI"
     gene            1952..2506
                     /locus_tag="PKM34_08060"
     CDS             1952..2506
                     /locus_tag="PKM34_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNQ83582.1"
                     /translation="MNDTSRQFGLAITACKEVFVNKMKDYGSSWRVLRPESLTDQIYI
                     KANRIRSIEQKGQQKVQEGIRPEFIGIVNYSVMALVQLELGIGGNMDLAPEEAGQLYD
                     KHLQSARALMEDKNHDYDEAWRNMRVSSYTDIILMKLMRIKQIEDREGETLVSEGVDA
                     NYRDIINYALFALIKLDSVGQLDS"
     gene            2648..3757
                     /locus_tag="PKM34_08065"
     CDS             2648..3757
                     /locus_tag="PKM34_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNQ83583.1"
                     /translation="MKLFANICRLLVAAVFLFSGFVKGVDPLGTAYRIEDYFIVFGTG
                     WAIPLSLFLSIFLCAVEFTLGAALLFNFRLKIMAWPLLLMMAFFTILTLNDAIYNPVP
                     DCGCFGDALKLTNWETFYKNVVLIILVIIIFAYRNKYRSIFVPRIDNWGLVIVFLLFS
                     YFSFHQYRHLPWIDFLGWKVGADLVPDNPGQAKTYLMYRNIATGEEKEYLSPNYPWND
                     TVWQKEWEFVSQRTDDSGVLKGHSLQIFDAEGNDLTEVYVNNPGYQFLVASYDLKSAS
                     VKGFKKIDKLHADMVSDGHSLVVLTGSLETDIREFKKGFQPDLEFFNADDVELKMMVR
                     ANPGIILLNDGVVLGKWHWRDLPDYEELKEEFEGL"
     gene            3757..>4072
                     /locus_tag="PKM34_08070"
     CDS             3757..>4072
                     /locus_tag="PKM34_08070"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83584.1"
                     /translation="MIKFILNRLFYGLLVLLGVITLIFILFNILPGDPARMLLGQRAD
                     ISSVEMIRKDLGLDKPLPVQYAGFLNDISPVSGHNHVDQTSYWYLDKEKYSPFLTVLK
                     IGK"
BASE COUNT         1023 a         1050 c          997 g         1002 t
ORIGIN      
        1 caaaaagatt tgaaataaaa aggggttatt gctttcagac tgtttcagtc accgcatttt
       61 cccgctcttt gggaaccgct tgcagtttct cttccagcag atcgcgcagg gccgacggct
      121 ggacattccg caccagttca ccgctttcgg cataggaaat ctttccggtt tgttccgaca
      181 cgacaatcgc aatggcatcg ctctgttccg ttacgccaac ggctgcccgg tggcgcaggc
      241 ccagcccggc ggtcaggtcc tggctcttgg agacgggcaa aatgcagcgg gccgcttcaa
      301 tcttattgcc gctgatgatc acggcgccat cgtgcagcgg gctgttcttg aaaaagatgt
      361 tttccagcaa ttcgactgag atctctgcat tcaggtactg gccggtatcc ttgatgatct
      421 tcagttcgtg cagccgggtg atgatgatga tggccccctg cttgctgttg gccattttct
      481 ggcaggcatg gacgatctta tcaacatcga caacataagg gctggcttcg ttgaaatgcc
      541 agaaaaggaa ccggcgacga cgtttctgga tgaaatcggg cttgcctaat gtcaggagga
      601 actgcctgag ttcgggctgg aaaatgataa acaaggcaat gatacccacg ctgaagaaag
      661 cccccaggat ttcgcggagc atctccatct gcagcgcgtc ggtgacccgc cagataaaga
      721 acatcgccac gatggcaaag aacatgttga tagagaccga acctttcagg aggttataaa
      781 gttcatacag gataaacgca accagcagta tgtcaagtac atccagcagg cgaatctgga
      841 acaagccggt aagaaggatt gcaagcatgg gatacaaaag tagggaaatt aggtaatttg
      901 aaaattattc aatgtgagaa tttgataata acttcattat cacatcatca cattatcaaa
      961 ttgcttttat gaacagtttc accgtctccg tcgcctcctt cacatcgtgc accctcagga
     1021 ttgacgctcc tttcatcaat gcaatagtat taagcaccgt agtgccgttt aaagcttccg
     1081 caggtttagt ccccaagact ttattgatca tggatttgcg cgacaggccg gcaaggacgg
     1141 ggtaacccgt atcaacgaat tcatccaggt gcgacaggat ttgataattg tgctctacgg
     1201 tctttccgaa gccgaacccg ggatcgataa tgattttatg atggccggcc gactccagtc
     1261 ttcggacctg gccaagcagg aaatccttta cctccatcac cacatcggca taggtcggat
     1321 tctgctgcat ggtggcgggc gtcccctgga tgtgcatgat gatgtaagga atttttagtt
     1381 taatgataca cgaaagcata agggggtcga acattcctcc ggagatatcg ttaatcatat
     1441 cagcgccttc accggccgct atttcagcaa tttccgaccg gtacgtgtca attgaaataa
     1501 taatctccgg aaatgatttt cttacttcct taagaaccgg caacaatctg ctcttctcct
     1561 cttcaacgct tacttcaggc gctcccggcc gcgtcgaaac cgcccctata tcgataatat
     1621 acgctccact atcagccatt tccttcacac gccccattaa atcgccaaat cgtccaaccg
     1681 ccaaatcgcc aaaccgccca accgccaaat cgccaaatcg tccaaccgcc aaatcgtcca
     1741 atcgcccaat cgtcaaatcg ccaaatcgcc caaccgtcca atcataaaag ctgtcaggcg
     1801 tcacattcac gattcccatc acccgcggca cgcgaaagtc catcgtcttg ccgttgtatt
     1861 caaaaacttt ggtttcggaa aaagatgtat ttttatcaac ctttttgatc atatttaacc
     1921 ggattaatta attgcgaaga taatagaaat gatgaacgat acctcccggc aattcggact
     1981 ggcgatcact gcctgtaaag aagtgtttgt caataagatg aaagactacg ggagttcgtg
     2041 gcgggttctg aggcccgaat cgctgaccga ccagatctat atcaaggcca accgcatccg
     2101 gtcgatcgag cagaaagggc agcaaaaagt gcaggaaggg atccggcccg agttcatcgg
     2161 gatcgtgaac tactcggtga tggcgcttgt tcagcttgaa ctgggcatcg ggggcaatat
     2221 ggacctggcc ccggaagagg ccgggcagct ttacgacaag catctgcaaa gcgccagggc
     2281 actgatggaa gacaaaaacc acgattacga cgaggcctgg cgcaacatgc gggtgagctc
     2341 ctataccgat attatcctca tgaaactgat gcggatcaaa cagatcgaag acagggaagg
     2401 ggagacgctg gtgtcggaag gggtggatgc gaattacagg gatattatca attacgcatt
     2461 atttgcttta ataaaactgg attcagttgg acagttggac agttgacagc ttgtcccgag
     2521 cgaagcgagg gattggacaa ttggacagtt ggacaataga aattagaaat tagtaatttg
     2581 aacttggaac caggaacttg gaactttgga acctggaact ttggaaccct ggaactaatt
     2641 gactcatatg aaactctttg ctaacatctg ccgtctgctc gtagccgctg tattcctctt
     2701 ttcaggattt gtcaaaggcg ttgatccatt gggtaccgct taccggatcg aagactactt
     2761 tatcgtcttc ggcaccggat gggccatccc gttgtcgctc ttcctgtcga tctttctctg
     2821 tgccgttgag ttcacgctgg gcgccgccct cctgttcaat ttccggctga agatcatggc
     2881 ctggccgctg ctgctgatga tggctttctt tacgatcctc acgcttaacg atgccatcta
     2941 caatcccgtt cccgattgcg gctgctttgg cgatgcgctg aaactgacca actgggaaac
     3001 cttctacaaa aatgtcgtcc ttatcatcct ggtcatcatc atatttgctt accggaataa
     3061 ataccgttcc atatttgtcc cccggatcga caactggggt ttggtcatcg tattcctgtt
     3121 attcagctat ttctcctttc accagtaccg ccacctcccg tggatcgatt tcctgggctg
     3181 gaaggttggc gccgacctgg tccccgacaa tcccggccag gcaaagactt accttatgta
     3241 ccgcaacatt gctaccgggg aagagaaaga gtacctctcc ccgaattacc cctggaacga
     3301 cacagtatgg cagaaggaat gggaattcgt gtcgcagcgg accgatgact ccggcgtcct
     3361 gaagggccat tcgcttcaga tcttcgatgc ggaaggcaac gacctcacgg aagtctatgt
     3421 gaacaatccc gggtaccagt tcctggtagc ctcgtacgac ctgaaatcgg cttctgtcaa
     3481 gggctttaaa aagatcgaca agcttcatgc ggacatggtt tcggatggtc attcgctggt
     3541 ggtgctcacc ggcagcctcg agaccgatat ccgggagttc aaaaagggtt tccaacccga
     3601 cctggaattc ttcaacgccg atgatgtgga gttgaagatg atggtgcggg ccaacccggg
     3661 gatcattttg ctgaacgacg gcgtggtatt gggtaaatgg cactggcggg atctgccgga
     3721 ttatgaagaa ttgaaagagg agtttgaagg attgtagtga taaaattcat cctcaaccgg
     3781 ttattctacg gactgctcgt cctcctgggc gtcatcaccc tcattttcat cctcttcaac
     3841 atcctccccg gcgatccggc acggatgctg ctgggccagc gggccgatat ctcttccgtc
     3901 gaaatgatcc gcaaggacct cgggctcgac aaaccgctgc ctgttcagta cgccggcttt
     3961 ttgaatgaca tctccccggt ttcagggcac aaccatgtgg accaaaccag ctattggtac
     4021 ctggataaag aaaaatactc tccctttctt acggtgctga agatcgggaa aa
//
LOCUS       DAOQXS010000273         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22715_length_4044_cov_3.089496, whole genome shotgun sequence.
ACCESSION   DAOQXS010000273 DAOQXS010000000
VERSION     DAOQXS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22715_length_4044_cov_3.089496"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKM34_08075"
     CDS             complement(<1..310)
                     /locus_tag="PKM34_08075"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNQ83585.1"
                     /translation="MTIEEIIRKALVEDLGGGDHTSLATIPVGATGRSRLLIKQEGIL
                     AGIDVAREVFRQVDPRLAFEKLLDDGAAIRPGDVAFYLSGPGISMLSAERLALNFMQR
                     M"
     gene            complement(321..725)
                     /locus_tag="PKM34_08080"
     CDS             complement(321..725)
                     /locus_tag="PKM34_08080"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNQ83586.1"
                     /translation="MRAILLPLILFVLAQSLPASLKAQDEPFDDICKAIRESDARSLA
                     AFFSATIEISLPDAENTYSASQGEMVMKDFFNRIPADNLTLVQKGSLNTSSHYSISTY
                     HSGGKNYQLYMEIRKEKEKFRLFKIKLEEKIK"
     gene            829..1533
                     /locus_tag="PKM34_08085"
     CDS             829..1533
                     /locus_tag="PKM34_08085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83587.1"
                     /translation="MNIVVTGTSRGIGYETVKLFAAAGHNKILAVSRHKARLEKLRSD
                     CEKENETSKIFIVDVDIAGDNFESRVVREVRENLGTVDILLNNAGALVNEPLDRLTKG
                     DFDLMFGVNVKSAFLMIQNLLPYFNPGAHIVNISSMGGVQGSVKFNGLSLYSASKGAL
                     AVLTECLALELKDSGISVNCLALGAAQTEMLAEAFPGYQAPLGATEMAGFIADFSMNG
                     HKWFNGKILPVALSTP"
     gene            1547..3832
                     /locus_tag="PKM34_08090"
     CDS             1547..3832
                     /locus_tag="PKM34_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ83588.1"
                     /translation="MRRKILVLTVLMHFMLSGANAGKIVHVYEFNRPEISYSGPYGIV
                     YFEGTKLAGQTGKATLPFYPVKILLPPGEMAESISISYKDPVVLQEKIMLMPFQEVRP
                     LSDPGESLMAFDETFYQSTTPYPSYFKPHLETQFFNGCGIALSSFTPISYLPKENQIT
                     YYQRVEIEIITLTDPAQSHNKHFFGSEQKSLWLKDLVQNPEKISDYFSGREFLTSEYD
                     ILFITKNQYLGEFDTLKSFYELQGLKTKVAATETIGSSMSGIDLQQKIRNFIIQEYDD
                     NGISYVVLGGDTEIVPYRGFYCYVQSGGGYSSTNIPADVYYSALDGSWNNDGDARWGE
                     PDEDDLYPEVAIGRITFSDTAELHNILHKSFLSQCDPVEGELTKPLLAGEELYDDPYT
                     IGSDYLDLLIGYRTDNGYSTHGIPPSHPRDTLYDDSTYYWSKATLISHINSGRPWLHH
                     VGHANQSYVMKLSLVDITNANFAQTNGVTHNYTVVYTHGCICGGFDYTDCIGERMIGI
                     DNFASAFVGNSRYGWFNQGTTDGPSQHLHREFMDALYSDSLYRIGMAHLKSKSETAPF
                     VEIGNEEFEPGATRWCFYDNNVLGDPVMALRTEEPQGIALVSETMVPASSASVAVQLE
                     SSTGNVKGLSCALMKNDTLYGSALTDGSGLAVIYPDSVLAEGEYHLWITGYNILPQMI
                     PIHACNYWLGYSPDWGNPANWFTGHIPDSTSYIIIPSQPAGTKFPSFNSSTERHCRHF
                     MIEENAQFRIGPGETLKVWGD"
BASE COUNT         1015 a          998 c         1007 g         1024 t
ORIGIN      
        1 tcatccgctg catgaagttc agggccagcc gttctgctga aagcatcgag atgcctggcc
       61 cggacaggta aaaggccaca tcacccggcc ggatcgctgc tccatcgtcc agtagttttt
      121 caaaggcaag acggggatcg acctgccgga aaacttccct ggccacgtca atgcctgcca
      181 ggatcccttc ctgcttgatc agcagccttg accgccccgt tgcgccgacg gggatcgttg
      241 ccagcgaggt gtgatccccg ccgcccaggt cttctaccaa cgctttccgg atgatttctt
      301 cgatcgtcat cttagcgcaa ttattttatt ttttcttcca gtttaatttt gaacaaccgg
      361 aacttttcct tctctttccg gatttccata taaagctggt aattcttacc tcccgagtga
      421 tatgtagaaa tcgaataatg agaagaggtg ttgagcgaac ctttctggac aagagtgaga
      481 ttatcggccg ggatcctgtt gaaaaagtct ttcatcacca tctcgccctg cgaggcgctg
      541 taagtgtttt cagcatcggg aaggctgatc tctatcgtag cactgaaaaa tgctgccagg
      601 cttcgtgcgt ccgattcgcg gatagctttg cagatatcgt cgaacggttc gtcctgtgcc
      661 tttaatgagg ccggtaagga ctgcgccagg acaaacagaa tcaacggaag aagtattgct
      721 ctcatacccg gattttcatg caaaattaca ataattgtat cgtcaggtgt aatattcgtt
      781 gcgatgaaca gagcgttttt actatttttg taatataata tcgaatatat gaatattgtt
      841 gtcacgggaa caagccgggg aataggttac gaaacggtaa aacttttcgc cgctgccggg
      901 cataataaga tactggcggt ttcacgtcat aaggctcgac ttgagaagct gcggtcggat
      961 tgcgaaaagg agaatgaaac cagtaaaata ttcattgttg atgttgatat tgctggcgac
     1021 aatttcgaat cccgggtagt tcgcgaagtc agggaaaatc tcggtacggt ggatatcctg
     1081 ctaaacaatg ccggcgcgct ggtgaatgag cccctggaca gattgacaaa aggggatttc
     1141 gacctcatgt tcggtgtaaa tgtaaaaagc gctttcctga tgatacagaa tcttttacct
     1201 tacttcaacc ccggtgcgca cattgtcaat atcagtagta tgggtggggt tcagggtagc
     1261 gtcaagttca atggcctgtc gctctatagt gccagcaaag gggcgctggc cgtgctgaca
     1321 gaatgtttgg ccctggaact gaaagacagc gggatcagcg tgaattgcct ggcactggga
     1381 gcggcacaga cagagatgct ggccgaagct ttccccggtt atcaggctcc tctgggtgca
     1441 accgaaatgg ccggctttat cgccgacttt tcgatgaatg gtcataagtg gtttaacggg
     1501 aagattttac ctgtcgcact gtcgactccg taacctgaat attccgatga gacgaaaaat
     1561 actggtttta acggttctga tgcacttcat gcttagcgga gctaacgccg gaaagattgt
     1621 tcacgtatat gaatttaacc gccctgagat cagttattcc ggaccatacg ggatcgtata
     1681 ttttgaagga acgaaactgg ccgggcaaac gggcaaagcg acactgccgt tttacccggt
     1741 gaagatcctt cttccgccgg gcgagatggc tgaatccata tcgatttcgt acaaagatcc
     1801 tgtcgtcctg caagagaaga tcatgctgat gcctttccag gaagtgcgtc ctctgtctga
     1861 tccgggcgaa tcactgatgg ctttcgatga aaccttttac cagagcacca caccctatcc
     1921 atcctatttt aagccccacc tggaaactca atttttcaac ggctgcggaa tcgccttgtc
     1981 ttcttttacc ccaatttctt atctcccgaa agaaaatcag ataacttatt atcaacgtgt
     2041 tgaaatagag atcatcaccc ttaccgaccc tgcgcaaagc cataacaagc actttttcgg
     2101 atcagagcaa aaatcactct ggctgaaaga cctcgttcaa aatccggaaa agatcagcga
     2161 ttatttttcc ggaagagagt tcttgacgag tgaatatgat atcctgttta ttacaaagaa
     2221 tcaatacctg ggcgaattcg atacgttgaa atccttttat gaattacagg gactgaaaac
     2281 caaagtggcg gctactgaga ccattggctc ttcgatgagc ggcatcgacc tgcagcagaa
     2341 gatcaggaat tttattatcc aggaatatga cgacaacggc atcagttatg ttgttcttgg
     2401 aggagatacg gagatagttc cttatcgcgg attttactgt tatgtgcaat cggggggcgg
     2461 ctattccagc acaaacattc cggccgatgt atattattct gcacttgacg gttcgtggaa
     2521 caacgacggc gatgcgcgct ggggcgagcc ggatgaggat gacctctacc ccgaagttgc
     2581 catcgggaga attactttca gcgatacggc cgaacttcac aacatcctgc acaaaagctt
     2641 tctttcccag tgtgatccgg tggagggtga gctgacaaag cctcttctgg ccggcgaaga
     2701 gctgtatgat gatccttata cgatcgggtc ggattacctc gacctgctga tcggttaccg
     2761 cacagataac ggctatagca cgcacggcat cccgccatcc catccgcgcg acacgcttta
     2821 cgacgactcg acttattatt ggtccaaagc caccctcatc agccacatca acagtggaag
     2881 accctggctg caccacgtgg ggcatgccaa ccagtcgtat gttatgaagc tttccctggt
     2941 tgatattacc aatgctaact tcgcgcagac caacggggta acccataatt acacggtcgt
     3001 ttacacccac ggttgtattt gcggtgggtt tgattatacc gattgtatcg gtgaaaggat
     3061 gatcgggatc gataatttcg cctccgcttt tgtcggtaac tccaggtacg gctggtttaa
     3121 ccagggtacc accgacggcc cttcgcagca tcttcaccgg gaattcatgg acgcattgta
     3181 ttccgacagc ctttaccgga tcggcatggc ccatttgaaa tccaagagcg aaacagcgcc
     3241 atttgtggag attggcaatg aggagttcga gcccggagct acgcgctggt gtttttacga
     3301 caataatgtc ctcggcgatc cggtgatggc gctccgtacc gaagaaccac agggtatagc
     3361 actggtcagc gagaccatgg tgccggcttc ttccgcttca gtcgcagtgc aactggaatc
     3421 ttcaaccgga aatgtcaaag ggctttcctg tgcgctgatg aaaaacgaca cgctttatgg
     3481 cagcgcactg accgatggat cgggcctggc ggttatctat cccgactcgg tgctggccga
     3541 gggcgaatac catctctgga ttaccggcta taacattctg ccccagatga ttcctatcca
     3601 tgcctgcaat tactggctcg gctattcacc agactggggc aatcctgcaa actggttcac
     3661 cgggcacata ccggatagta cgtcgtatat tattattcca tcccaaccgg ccgggacaaa
     3721 attcccctcg tttaactcga gcacagagcg acactgccgt catttcatga tagaagagaa
     3781 cgcacagttc aggataggcc ccggtgaaac tttgaaagtc tggggagatt gattttccgt
     3841 tatctttgca gccgcttaac ggaatagaat catggcttcc gacacgatca tccacccggg
     3901 tgttattgac catatcgaag gcgataaagc ttttgtgaaa atactgtcac agtcggcatg
     3961 cggttcctgc catgccaaag gaatgtgtac cgtagccgaa gtcgaagaga aaatcgtcga
     4021 agtcgggata aaccccggcg ctgc
//
LOCUS       DAOQXS010000274         4039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22755_length_4039_cov_3.996486, whole genome shotgun sequence.
ACCESSION   DAOQXS010000274 DAOQXS010000000
VERSION     DAOQXS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22755_length_4039_cov_3.996486"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1281
                     /locus_tag="PKM34_08095"
     CDS             163..1281
                     /locus_tag="PKM34_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNQ83589.1"
                     /translation="MALINSAPGPSRFDYIDQVRGLVVVLMLLDHCSYYFNSVWDQID
                     PLDPLFDSWPQFALRYASYLCAPGFLILAGAMVWWSYTKQIEKGIERKKIIRQIMIRG
                     LFLVVLQVTWVNSSWGGFSEFKPGHLGIIACIGISMILLALVIRYHWMVRAALGLLIL
                     ALHPLLLRIPYDPATGWQEALMQTFIDAGEFNKYPVIPWFALALLGSAMASGWLQAWK
                     SDRAKISWSFGIGAAAILLALLVRMGRGYGNIFPFSDPGSYSFFFDQKYPPSLFLNLW
                     FFGWVLVITGIFLAINKISPKILYFFAIPGRVALFFYGMHIAIMGIFIKRIDFLYRDG
                     GVTETLIAFAVMMVVMIPLCLWFYNVKKRSRNWVVRMI"
     gene            1391..2218
                     /locus_tag="PKM34_08100"
     CDS             1391..2218
                     /locus_tag="PKM34_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83590.1"
                     /translation="MIKSIKYYVLINGFAIILAILMVGMVGGCAEKRDGIPAQMTFSR
                     FYYEKSGRQAAISNVFEMKEGENVVAVVDPGVSLMDPGRDRLFHIDWIGPGNRSVYLK
                     RIDLPAGDSTGKLVSAVSLDPSKRDAGIYTVRVYYFRDLIAEKQFEMVPEGTANHIEA
                     DIIFYKSLDKETGEMNGIDTVFEIRKKGILRARVNLRNMGIYEDDELPFRMEWYGPDG
                     KSFYDKKMMVNPADTVATLNGSVSITPDKRQPGDYELRVLLFDDIIVKRRFFLRSED"
     gene            2370..3914
                     /gene="cysS"
                     /locus_tag="PKM34_08105"
     CDS             2370..3914
                     /gene="cysS"
                     /locus_tag="PKM34_08105"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNQ83591.1"
                     /translation="MQQLYIYNSLSRKKELFEPLVPGHAGMYVCGPTVYGDPHLGHAR
                     PAVTFDLVFRYLKHIGFKVRYVRNITDVGHLEGDADAGDDKIGKKARLEQLEPMEVVQ
                     YYTNRYHELMARLNLLNPSIEPHASGHIIEQIAMVQKILDAGFAYERNGSVYFDIEKY
                     NQKHPYGILSGRKVDELLAFTRELDGQEEKKSSFDFALWKKATPDHIMRWPSPWGEGY
                     PGWHLECSAMGCKYLGETFDIHGGGMDLLFPHHESEIAQSMAATGKMPVRYWMHNNMI
                     TINGQKMGKSLGNAVSLEDFFNGNHSLLEKAYSPMTIRFFILQAHYRGTLDFSNEALQ
                     ASEKGLRKLLAALAIVQSSKFKVQNLQKLKFGNVYYEDLELWNPEPGTVTGPIAQIIA
                     NSYSAMNDDFNSPIVIAQLFEAARIINLVKEGKEKLSDNDMAALAFAYRTFMVDILGL
                     VPEEQSGQKDEIISGLMDTIIGIRADARAKKDFATSDQIRDKLLKVNIQIKDGKEGAT
                     WEIKED"
BASE COUNT         1001 a          976 c         1098 g          964 t
ORIGIN      
        1 cggctcagct tggcggaatg cagatcatcg atgtgaccaa tccggcggcg ccaaagctga
       61 tcggcgcggt caagacaaaa tatgccctgg gtatcgacgc cgatgaccag tatatctatc
      121 tggccgatga agatgaaggg gtgttgatca ttagcattcc ttatggcctt gattaattca
      181 gcaccgggac catcgcgttt cgattacatc gaccaggtcc gcggcctggt agtggtcctg
      241 atgctgctcg accattgttc ctattatttt aactccgtct gggaccagat cgatccgctg
      301 gatccgcttt tcgacagctg gccccagttt gcccttcgct acgcctccta tctctgtgcc
      361 cccgggttcc tgatcctggc aggggccatg gtctggtggt cgtatacaaa acaaatcgaa
      421 aaagggatcg aaaggaaaaa gatcatccgg cagatcatga tccgggggtt attcctggtc
      481 gtgctgcagg ttacctgggt caactcctcc tggggaggat tcagcgaatt caaaccgggc
      541 cacctgggga tcatcgcctg cattggaatc tcgatgatcc tgctcgccct ggtcatccgg
      601 tatcattgga tggtcagggc cgcactggga ctgctgatcc tggccctcca cccgcttttg
      661 ctccgcattc cgtatgatcc ggcaacaggt tggcaggaag ccctgatgca gacctttatc
      721 gatgcggggg agtttaacaa atacccggtc atcccctggt ttgcccttgc cctcctggga
      781 tcggccatgg ccagcggatg gctgcaggcc tggaaaagcg accgggcaaa gatatcctgg
      841 agcttcggta tcggagcggc agcaattctg ctggccctcc tggttcggat gggcaggggc
      901 tacggcaata ttttcccatt ctccgacccc ggttcctatt ccttcttctt cgatcagaaa
      961 tatccgccaa gcctcttcct gaacctctgg ttcttcggat gggtgctggt catcaccgga
     1021 atcttcttag ccatcaataa aatctccccg aaaattcttt actttttcgc cattcccggg
     1081 cgcgtggcgc tgtttttcta cggaatgcac atcgccatca tggggatttt tatcaaacgt
     1141 attgatttcc tttacaggga tggcggggtc accgaaacat tgatcgcatt cgctgtaatg
     1201 atggtcgtga tgatccccct ttgcctgtgg ttctataacg tgaaaaaaag aagcaggaac
     1261 tgggtggtga ggatgatatg agggtaggac ggtaggacgg taagacttta ggacggtaag
     1321 actgcaggac ggcagggttt taagggtttg gagggttgtc aggatgagtg gtaagttatt
     1381 caatttaagg atgataaaga gtatcaaata ttatgttttg attaatggtt tcgcgataat
     1441 ccttgcgatt ttgatggtgg ggatggttgg aggatgcgcc gaaaaaaggg atggcatccc
     1501 tgctcagatg acgttcagcc gcttttatta cgaaaaatcg ggcaggcagg ctgcaattag
     1561 caatgttttt gaaatgaagg aaggggagaa tgttgtggct gtggttgatc cgggtgtttc
     1621 cctgatggat ccggggcgcg accggttgtt ccatatcgac tggatcgggc ccggcaaccg
     1681 gtcggtctat ctgaaaagga tcgatctccc ggccggggat tcgaccggca aactggtcag
     1741 tgcagtctcc ctcgacccct caaaacgaga tgccggaatt tataccgtaa gggtttatta
     1801 tttcagggat ctgatcgcgg aaaagcagtt tgagatggtt cccgaaggaa cagcgaatca
     1861 tatcgaggct gacatcatct tctataaaag tttggataaa gaaacagggg agatgaatgg
     1921 catcgatacg gtatttgaga tcaggaagaa agggatcctc cgggcccggg ttaaccttag
     1981 gaatatgggt atttatgaag atgatgaatt gcccttccgg atggaatggt acggcccgga
     2041 tggaaaatcg ttctatgaca aaaagatgat ggtcaatcct gcggatactg tcgccacatt
     2101 aaatggttct gtatccatta ccccggataa acggcaaccg ggtgattatg aattgcgtgt
     2161 gttgttgttt gatgacatca tcgtcaagcg aaggtttttt cttagatcgg aggattgatt
     2221 taaccacaaa ggcgcacgaa ggatattcac aaaggcgcac gaagcatcag tttaaaagca
     2281 aagtgccaga cagttttaca ggaccactcg acgaaatcgg ataaaccgat tgctattaat
     2341 cactattttt gcctggaatc ttacttgata tgcaacaact ttatatctat aacagccttt
     2401 cccggaagaa ggaacttttc gagccactgg taccgggcca tgccgggatg tacgtctgcg
     2461 gtcccaccgt atacggcgat ccgcacctgg gccatgcccg gccggctgtg actttcgacc
     2521 tggtgttccg ttacctgaaa catatcggct tcaaggtgcg ctacgtgcga aacataaccg
     2581 acgtcgggca cctggaagga gacgccgatg ccggcgacga taagatcggc aaaaaggccc
     2641 ggcttgaaca gctcgaaccg atggaggtgg tgcaatatta caccaaccgc tatcacgaac
     2701 tcatggcgcg gctgaacctg ctgaatccga gcattgaacc gcacgcatca ggccatatta
     2761 tcgagcagat cgccatggtg cagaagatcc tcgacgccgg ctttgcttac gagcgcaacg
     2821 gctctgtgta tttcgacatc gaaaaataca atcagaaaca tccatacggc atcctttcag
     2881 gacgtaaagt ggatgaatta ctggctttta caagggaact cgacgggcag gaagagaaaa
     2941 aaagcagttt cgattttgcc ctgtggaaaa aggccacccc cgatcatatc atgcgctggc
     3001 cctcgccctg gggcgaaggt taccccggct ggcacctcga atgttcggcc atgggatgca
     3061 aatacctggg cgagacgttc gacatccacg gcgggggaat ggatctgctt ttcccgcacc
     3121 acgaatcgga aatagcccag tcgatggcag cgacgggcaa gatgccggtg cgttactgga
     3181 tgcacaacaa catgatcact atcaacgggc agaaaatggg taaatctctg ggtaatgccg
     3241 tttcgctgga agattttttc aatggcaacc acagcctgct ggagaaagct tacagcccga
     3301 tgacgatccg ttttttcatc ctgcaggccc actaccgcgg caccctcgat ttctccaacg
     3361 aagcgctgca ggcgagcgaa aaaggactgc gtaagctgct cgctgcgctc gcaatagttc
     3421 aaagttcaaa gttcaaagtt caaaatttgc agaagttgaa atttggaaac gtgtattatg
     3481 aagatttgga actttggaac ccggaacccg gaactgttac cgggccaatt gcacagataa
     3541 ttgccaatag ctactcggcg atgaacgacg atttcaacag cccgatcgtg atcgcccagc
     3601 ttttcgaggc ggcccggatc atcaaccttg ttaaagaagg caaagagaag ctttccgata
     3661 acgacatggc ggcgctggcc tttgcctacc ggactttcat ggtcgatatc ctgggcctgg
     3721 tgccggaaga gcaatcggga caaaaagatg agatcatcag cgggctgatg gacaccatta
     3781 tcggcattcg cgcggatgcc agggcgaaga aggatttcgc cacctccgac cagatcaggg
     3841 ataagttgct aaaggtcaat atacagatta aggacgggaa ggaaggggcg acgtgggaga
     3901 ttaaggaaga ttagacgatt ccgcattttt cgacaatgcc gaatgcgcaa tgccaaatcg
     3961 cgtaatgata ttgtccaact gcgatccttc gtccgccttt ggcggactca ggacatgctg
     4021 cccaactgtc caactgtca
//
LOCUS       DAOQXS010000275         4023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22864_length_4023_cov_4.050403, whole genome shotgun sequence.
ACCESSION   DAOQXS010000275 DAOQXS010000000
VERSION     DAOQXS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22864_length_4023_cov_4.050403"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKM34_08110"
     CDS             complement(<1..1074)
                     /locus_tag="PKM34_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83592.1"
                     /translation="MKKIHLLYIVPLVLLAACKPNIDEFAPSKGNADFSSFVAVGDSW
                     TSGFADGSLYISGQEYSFPNIMNGQFKKAGGGDFLQPLMLDEYGIGLATGIPKPKLEM
                     GFHPDCKGAVGLVPAYADVPVNMDNLNPLNTGIHYDNISTPGLKTFYMGVPGMNQLNP
                     YYKRFASEGAATMNDEIPLAAPTFFSLWLGSYDALAYAIAGGADPMNPVTTKEVFAGS
                     LQQTLAALTATGAKGVMANIPDVTDFPFFHTIPYNALPLDQASADALNAGYAQVNAII
                     KGLGRTDTIHFKSGANAVIIADATLPWGFRQVKPTEFLLLSLPQDSLKCAGWGSQKPI
                     PGQYVLDEAEIAITKNAVDEYNSQ"
     gene            complement(1093..2307)
                     /locus_tag="PKM34_08115"
     CDS             complement(1093..2307)
                     /locus_tag="PKM34_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNQ83593.1"
                     /translation="MKNKFTLIFALCLWSVATLGGGYQVRLQGHKQTGFGLIGTPFAH
                     DASSIFYNPGSLAFYEGKFSISAGASGIFSNVLFQMQGTDYQARTDNPTGTPFYGYVS
                     YKFKDMIAIGVGVYTPFGSTTKWDENWAGRYLIRDIKLSAIYIQPTIAYNYKDKFGIG
                     AGFVYATGSVELNKALPYSPDSKVNLSGKADNFGFNAGLFFKPTEKLNIGIDYRSEIS
                     MDLEAGDATFTVPVALQTMVPDTNYFDASLPLPANLDFGIAYQVNDKLTLAAEVNWII
                     WSSYESLDFTFENNPELLNSENPREYKDTWIPRIGGEYKLNDIFTLRAGAYFDPSPAS
                     EEYFTPETVTLNTIAFTLGASITPLKGLDIDISYLELHGLEAEKNYSPDNFAGTYETA
                     AFIPGIGLSYRF"
     gene            complement(2386..3402)
                     /locus_tag="PKM34_08120"
     CDS             complement(2386..3402)
                     /locus_tag="PKM34_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNQ83594.1"
                     /translation="MKKVLGLGNALVDIMTQLDHDDYLSAFELPKGSMILVDARRSHK
                     IYDDTIHLQKTVRSGGSAANTIHGLANLGVETGFIGKIGRDEMGKVFHDDMITAGINT
                     HLSQDDTATGRAIALISPDTERTFATYLGAAVELNRNDLSRETFSQYDFFHIEGYIVQ
                     NHSLLESALKMARECRMTISLDLASYNVVAENRDFLEEMIRKYVDITFANEDEARYLT
                     GLEPEEALEVLAGMCEIAVVKVGREGSIVGSRQSAVGSRQSAVGSRQIKIGVIPVKSV
                     DTTGAGDLYASGFLYGLANGYDLEKCGRIGAVAAGKVIEMIGPKLNDQKWREVRDFIR
                     FNIL"
     gene            complement(3498..>4023)
                     /locus_tag="PKM34_08125"
     CDS             complement(3498..>4023)
                     /locus_tag="PKM34_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83595.1"
                     /translation="QQLDQLIAEKEVLRRDLDNTIQVKDQLATQVNWLDSAVDRQNSI
                     IAGQQQELTEKEKALHTAYYRIDTYKTLRDQNILEKEGGFLGINRVTTLADQVDNNLF
                     SRIDTREVVRIPVAAKQWEIVTGQDPASYDVVFESKNNVEWINITDPEKFWGKSKYLV
                     VVVRENSSELASNR"
BASE COUNT          877 a         1037 c         1097 g         1012 t
ORIGIN      
        1 ctgcgaattg tattcgtcga cggcattttt cgtgatggcg atctcggctt catccagcac
       61 atactggccg gggatgggct tctggcttcc ccatccggcg catttaagcg aatcctgcgg
      121 caggctcagc aacaggaatt ccgtcggttt gacctggcgg aatccccagg gcaaggtagc
      181 atcggcaata attaccgcat ttgcacccga cttgaaatgg atcgtatcgg tccttcccaa
      241 acccttgata atggcattta cctgggcgta tccggcattc agcgcgtcgg cgctggcctg
      301 gtcgagcgga agggcattgt aggggatggt gtggaagaac ggaaaatcgg tcacatcggg
      361 gatgttggcc atcacgcctt tggcaccggt tgccgtcagg gcggccaggg tctgttgcaa
      421 tgatcctgcg aacacctctt ttgtcgttac cgggttcatc gggtcggcgc cacctgcgat
      481 tgcgtaggcc aaagcatcgt agctgcccag ccagagcgag aagaaggtgg gggctgccag
      541 tggtatctca tcattcatag tggctgcccc ttccgaagca aaccttttat aatacggatt
      601 aagctggttc atgccaggta cacccatata gaacgtcttc agcccgggtg tggagatgtt
      661 gtcgtagtgg atgccggtat tcagagggtt caggttatcc atattgacag gcacatcggc
      721 gtaagccggg accagtccta cagctccttt gcaatcggga tgaaatccca tttcgagctt
      781 tggcttcggg atgcctgttg ccaggccaat cccgtattca tcaagcatga gaggctggag
      841 gaaatcgcca cccccggctt ttttaaactg tccgttcata atgttcggga aggagtattc
      901 ctgcccggat atgtacaggg agccatcggc aaacccggat gtccaggaat ccccgacggc
      961 cacaaagctg ctgaaatcag catttccctt cgaaggggca aattcgtcga tgttcggctt
     1021 gcaggctgcc agcaatacca gcggaacgat ataaagcaaa tgtatctttt tcatattcgt
     1081 gagtatttta ggttaaaaac ggtagctgag gccgatgccg ggaatgaaag cggctgtctc
     1141 gtaggttccg gcaaaattat cggggctgta attcttttct gcttcgagcc cgtggagttc
     1201 gaggtaggaa atatcaatat caagcccttt cagcggtgta atggaggcgc ctaaagtgaa
     1261 ggcgatggta ttgagggtaa ccgtttcggg agtaaaatac tcctcacttg ccggggaggg
     1321 atcgaaatag gccccggcgc ggagggtgaa aatgtcgttc agcttatatt cgcctccgat
     1381 gcggggaatc cacgtgtctt tgtattccct cggattttcg gaattcagca gttcgggatt
     1441 attttcaaat gtaaaatcaa gtgattcgta gctgctccag atgatccagt tcacctctgc
     1501 cgccagtgtc agcttgtcgt tgacctggta agctatgccg aaatcgaggt tggccggcaa
     1561 gggcagggag gcgtcgaaat aattggtgtc gggaaccatg gtctggaggg caaccggtac
     1621 ggtaaaagtg gcgtcgccgg cctcgaggtc catcgatatc tcggagcggt aatcaatccc
     1681 gatgttcagc ttctcggtgg gtttgaaaaa gaggcctgca ttgaaaccga agttgtccgc
     1741 ttttccagaa agattgacct ttgaatcggg gctgtagggg agagccttgt tcagttccac
     1801 actgccggtg gcatacacga acccggcgcc gatcccgaac ttgtccttgt aattgtaagc
     1861 aatggtcggc tggatgtaga tggcgctgag cttgatgtcc cggatcaggt atcggccggc
     1921 ccagttctca tcccacttcg tcgtactgcc aaaaggagta taaaccccta cgccgatagc
     1981 gatcatgtct ttgaatttgt aggagacata gccgtagaat ggcgtgcccg tcgggttgtc
     2041 ggtccgtgcc tggtaatccg ttccctgcat ctggaacagt acattgctga agatgccgct
     2101 tgcgccggcc gagatgctga acttgccttc ataaaacgcc agcgaaccgg ggttgtagaa
     2161 gatggagctt gcgtcgtggg cgaaaggggt gccgatcagg ccgaagcccg tttgtttgtg
     2221 tccctgcagg cgtacctggt agcctcctcc gagggtggca accgaccata ggcataatgc
     2281 aaagatcaga gtgaatttgt ttttcataag gtgagttatt ggttagaaaa agggcgcaat
     2341 ttacatattt attcaatctt ataacaaaat atgtaaaaaa ttgaattaca gaatattgaa
     2401 acggataaaa tcgcggacct cccgccattt ctgatcattc agcttgggcc cgatcatttc
     2461 gatcactttt ccggccgcca ccgctccgat ccgtccgcat ttttccaggt cgtacccatt
     2521 agccagtccg taaaggaacc cggaggcata cagatccccc gccccggtgg tgtcgacact
     2581 tttcaccggt ataaccccaa ttttgatctg cctgctgccg actgccgact gcctgctgcc
     2641 gactgccgac tgccgactgc cgactatcga cccctctctc cccaccttca ccaccgctat
     2701 ctcacacatc cccgccagca cctccagcgc ttcttccggt tcgaggccgg tcaggtatcg
     2761 ggcctcgtct tcgttggcaa aggtgatatc gacatactta cggatcatct cttccaggaa
     2821 gtcgcggttt tcggccacca cgttgtagct ggccaggtcg agcgaaatag tcatccggca
     2881 ctcgcgggcc attttcaggg cgctttccag gagcgagtgg ttttgaacga tatagccttc
     2941 gatgtggaag aagtcgtatt gcgagaacgt ttcccgggag aggtcgttgc ggttgagctc
     3001 aacggcagcg ccgaggtaag tggcaaaggt ccgttcagta tccgggctga tcagggcgat
     3061 ggcgcggccg gtggccgtgt cgtcctggct gaggtgggtg ttgatgcccg ctgtgatcat
     3121 atcgtcgtgg aacacctttc ccatctcatc ccggccgatc ttaccgataa aacccgtctc
     3181 cacccccagg ttggccagtc cgtgaatagt attggcggct gagcctccgg agcggacggt
     3241 tttttgcaaa tggatggtgt catcgtaaat cttgtgagag cggcgcgcat ccaccaggat
     3301 catgctccct ttcggcagtt cgaatgcgct gaggtaatcg tcgtggtcga gctgggtcat
     3361 gatgtcgaca agggcattgc cgaggccgag gactttcttc attgcggcag agggtttaga
     3421 gttttgctgc cggcaaagat aatggataaa aaaaagcggg tgacgggtct ccccgccacc
     3481 cgggtcgata tatagaatta cctgtttgaa gccagttcac tgctgttctc acgaacgaca
     3541 acaaccaggt acttgctctt accccagaat ttctcgggat cggtgatatt tatccactcc
     3601 acattatttt tactttcaaa aacaacatcg tacgatgcag gatcctgccc tgtgacaatc
     3661 tcccattgct ttgctgcaac cggtatccga acaacttcac gggtatcaat cctggaaaag
     3721 agattattgt caacctggtc tgcgagtgtc gtaaccctgt taattccaag aaatccgcct
     3781 tctttttcta gaatattctg atcccgcaat gttttatagg tatcgatccg gtaataggcc
     3841 gtgtgcaacg ctttttcctt ttcggtcagt tcctgctgct ggccggcaat gatgctattc
     3901 tgccggtcaa ccgcgctgtc gagccagtta acctgcgtgg caagctgatc cttaacctgg
     3961 atggtgttgt ccagatctct ccgcaacacc tctttttcgg caatgagctg atcaagctgc
     4021 tgc
//
LOCUS       DAOQXS010000276         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_22899_length_4020_cov_3.112736, whole genome shotgun sequence.
ACCESSION   DAOQXS010000276 DAOQXS010000000
VERSION     DAOQXS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22899_length_4020_cov_3.112736"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKM34_08130"
     CDS             complement(<1..502)
                     /locus_tag="PKM34_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83596.1"
                     /translation="MAQLLVRFILRNRLANLLVILAMTGFMGYMATGLKISYDFQQML
                     PYTDSISIDYHHFKEVFGEDGSVMFIGIKSDNLFELEEFNDWWDLTYSIKAMEGVGEV
                     VSLARIYQLEKDDSLRKLNFDLVFQGKPQSQAELDSLKNVVLSIPFYEGFLINKETGA
                     TLMMVTL"
     gene            626..1702
                     /locus_tag="PKM34_08135"
     CDS             626..1702
                     /locus_tag="PKM34_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83597.1"
                     /translation="MKIKIQFLLSFFILVMMNVIFGQAPRTLDEDINRLAGKIVQYPA
                     REKYLTEIKSKYDEACRIDRERIQELRAGGQPDIWYEIFRTYQVLENRQAVVKRIPDA
                     SLQKTGIVIADYSKEMDEAKYKATAYLYAHALKLLETGSVPDSRQAYAELMKAASLNN
                     SYKEIDKLIRKAVLKGASDVEFEMDNRTGKVVSSSMIRYLSVIVWEFKKANYGHEKPG
                     KPDKSFPFTLRVVLDELQIGKDQVRELQYQEERDVMRDFMIVDTIRCQISETRQLKKA
                     VLSGRLEYIDRLTGKVVNRVPVKVESVFTNAYATLQGNPDAAGDETRRLLKSSRVAYP
                     SNEQMILDATEEFAKKATEIILGE"
     gene            complement(1706..2923)
                     /locus_tag="PKM34_08140"
     CDS             complement(1706..2923)
                     /locus_tag="PKM34_08140"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNQ83598.1"
                     /translation="MKESIVILAGGGPAPGINTVISSIAKVFLKDGYRVIGMHEGYKT
                     LFTSNPKTQDFDFELADKIKNQGGSALVMSRHKPKDSEFNTDFFVANNVKLLVTIGGD
                     DTASTANRISQFLSNHDIKIQNIHVPKTIDNDLPLPEGQPTFGYQSAKEEGVRIASTI
                     YEDSRTSGNWFIISAMGREAGHLAFGIGGACHLPMIIIPEMFNKTDISFDKITRMIIS
                     SILKRKILGISYGVAIISEGVFHFMTDEEIESTNISFTYDDHGHPELGNVSKAHIFNL
                     LLQRKLKELNISVKSRPEELGYELRCISPTAFDLMYCSLLGLGVKILYDQGMSACMVT
                     SDPKGDVYPLFLKDVTDEKGKVKPRMVNINSQKVTMVYEHNLQFLTEEDYEAARQYVP
                     DPENFDFRKILNW"
     gene            complement(2983..>4020)
                     /locus_tag="PKM34_08145"
     CDS             complement(2983..>4020)
                     /locus_tag="PKM34_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ83599.1"
                     /translation="FPNDSKEPEIRTGAQWNELERITGSEVTGTAAEIYDRMIVFGEL
                     ISSVIVSEYLNRQGIENTWCDIRTVVRTDSVFRDASIDWEVSEKQSDAILKPLLKSVG
                     GIRPVVVTQGFIGSDDLGRSTSLGREGSDYTASVLASLLDASEVITWKDVPGILNADP
                     RHFSQTIKLERISYSEATELAYYGAKVLHPKTIKPLQNKNIPLQVRSFEEPDQPGTLI
                     QEETGVESLVPSFIFKFGQVLMSVSAKDFSFIDEKVFRDVFSLLSKYAIHVNIIQNSA
                     LSLSFCTDSHGHLVELIDGLRDDYIVKYNEGLELITIRHSNDRASEIVLSGREVLLEQ
                     HNRVVSQYVVR"
BASE COUNT         1020 a          987 c          999 g         1014 t
ORIGIN      
        1 tcagcgtgac catcatcagg gtggccccgg tttctttatt gatcaggaaa ccttcgtaaa
       61 acgggatcga aaggacgaca ttcttaaggc tgtcgagttc ggcctggctt tgcggttttc
      121 cctggaagac caggtcgaaa ttcagcttcc gcagactgtc gtctttttcc agctggtata
      181 tcctggccag agatacaacc tctccaaccc cctccatcgc cttaatagaa taggtgaggt
      241 cccaccagtc gttgaattct tccagctcaa aaagattgtc actttttatc ccgatgaaca
      301 tcaccgaacc atcttctccg aaaacttctt tgaaatgatg gtagtcgatg ctgatgctat
      361 cggtgtatgg caacatctgc tgaaaatcat aggatatttt caacccggtc gccatatacc
      421 ccataaaacc tgtcatcgct aagattacga gcaggttggc aagcctgttt ctaagaatga
      481 accggaccag taactgggcc atgaaagaaa aatattttta ctttataata gttttgcaca
      541 aaatttggtg caattgaaag tctctgcttt atcgcgggtt caaatttaat tttaattagg
      601 acacacaacg aaaaaattac cggtcatgaa aataaaaatc cagttcctgt tatcattctt
      661 tatattggtc atgatgaacg taatattcgg acaggctccc cggacgctgg acgaagatat
      721 taaccggttg gcgggcaaaa ttgtacagta tcccgccagg gaaaagtatt tgacggagat
      781 taagtcaaaa tatgatgaag cctgcaggat cgaccgggaa cggatccagg aactgcgtgc
      841 cggcggccag ccggatatct ggtatgaaat cttcaggacg taccaggtac tggaaaacag
      901 gcaggcggta gttaaaagga tccctgacgc ttcgctccaa aaaaccggaa ttgtaattgc
      961 cgattattcc aaggagatgg atgaggccaa atacaaggca acggcctatc tttatgccca
     1021 tgcgctgaaa ctgctcgaaa caggcagcgt acccgattca cggcaggcat acgccgaact
     1081 gatgaaagcc gcttctctga acaattccta taaagagatc gataaactga tccggaaagc
     1141 cgttctcaaa ggcgcttccg atgtggaatt cgaaatggat aaccgtacag gcaaagtggt
     1201 cagctcttcc atgatccgtt acctctccgt catcgtctgg gagttcaaga aagcgaatta
     1261 cggccatgaa aaaccgggga agcccgataa aagtttccct tttaccctcc gggtagttct
     1321 cgacgagctg cagatcggca aagaccaggt cagggaactc cagtaccagg aggagagaga
     1381 tgtgatgcgc gattttatga tagtcgatac gatccggtgc cagatttccg aaacccgtca
     1441 gttgaagaaa gcggtattat ccgggaggct ggaatacatc gaccggctga cgggcaaggt
     1501 ggtcaaccgg gtgccggtga aggtggaatc ggtttttacc aatgcgtatg ctaccctgca
     1561 gggaaatccc gatgcagccg gcgatgaaac ccgccgcctg ctgaaatctt cacgggtggc
     1621 ttatccttcc aatgaacaaa tgatcctcga cgcaacggag gaatttgcaa aaaaagcgac
     1681 tgaaattatc ctgggtgagt aggacctacc agttcaggat ctttctgaaa tcgaaatttt
     1741 caggatcggg aacgtattgc cgggccgcct cgtaatcctc ttcggtaagg aattgcaggt
     1801 tatgctcata aaccatggtc actttctgtg aattgatgtt gaccattctc ggttttactt
     1861 tgcccttttc atccgtgaca tctttcagga aaagcggata tacgtcccct ttgggatcgg
     1921 aagtgaccat acaggccgac atcccctggt cgtagaggat ctttacgcca agcccgagca
     1981 atgagcagta catcaggtcg aaagcggtcg ggctgataca gcggagttcg taccccagct
     2041 cttccgggcg gcttttaacc gagatattca gttcctttaa ctttctttgc aacagcaggt
     2101 taaagatatg cgccttgctg acgttgccca gttccgggtg gccgtgatcg tcgtaagtga
     2161 agctgatatt ggtcgactcg atctcctcat cggtcatgaa atggaaaacg ccttcgctga
     2221 tgatggcaac gccataactg atccccagga tttttctttt caggatggaa gagatgatca
     2281 tccgggtgat tttatcgaag gaaatatcgg ttttgttgaa catctcaggg ataatgatca
     2341 tgggaagatg gcatgcgccg ccgatcccga aagcgagatg ccctgcttcg cgtcccatgg
     2401 ctgagatgat gaaccagtta ccgctggtcc gggaatcctc atatatggtc gatgcgatac
     2461 ggacgccctc ctccttggcc gactggtaac cgaatgtcgg ctgaccttcc ggtaagggca
     2521 gatcgttatc gatcgtcttc ggaacgtgga tgttctgaat tttgatgtcg tgattcgaca
     2581 ggaactgtga gatcctgttg gccgtcgaag ccgtatcatc cccgccaatg gtgaccagca
     2641 gttttacatt gttggccaca aaaaaatcag tgttgaattc gctgtctttc ggcttgtggc
     2701 ggctcatgac cagcgccgaa ccaccctggt ttttgatctt atcggccagt tcaaaatcaa
     2761 aatcctgtgt cttcggattg gacgtgaaca aagttttata tccttcatgc atcccgatga
     2821 cacggtaacc gtctttcaga aaaaccttgg caatgctgct gataacggtg ttgatgccgg
     2881 gtgcgggccc gccgccggct aaaatgacaa tggattcttt catatacgtg ggaaagtttg
     2941 tgtttgaagg tgcaaaaata gcaagatttg cgagacgcgg ccctatctga ctacatattg
     3001 cgaaaccacc cggttatgtt gttccagcag tacctcccgg ccgctcagaa ctatttccga
     3061 tgccctgtcg ttcgaatggc ggatggtgat cagctccagc ccttcattgt attttacgat
     3121 gtaatcgtca cgaaggccgt caatcaattc aaccaggtgc ccgtggctgt cggtgcagaa
     3181 cgaaaggctc agggccgagt tctgaataat attcacatgg atggcatatt tcgataaaag
     3241 ggagaaaaca tcccggaata ctttttcatc gataaaagaa aaatcttttg cggaaacact
     3301 catcagcacc tgtccgaatt tgaagataaa actggggacc agcgattcga cgccggtctc
     3361 ttcctgtatc agggtgccgg gctggtcggg ttcttcgaac gacctgacct ggagaggtat
     3421 gtttttgttt tgtaacggtt ttattgtttt aggatgaagg actttggcgc cgtaataggc
     3481 cagctctgtc gcctcgctgt aagaaatccg ctccagcttg atggtctggc tgaaatgcct
     3541 gggatcggca ttcaggatgc cgggcacatc tttccaggtg atcacctcac tggcatcgag
     3601 cagggaggcc agcaccgaag cggtataatc cgacccttcc cgacccagcg atgtgctccg
     3661 ccccaggtca tcgctgccga taaacccctg tgtcacgaca accggccgga tgcctccaac
     3721 gctctttagc agcggtttca aaatggcatc cgactgcttt tcactcactt cccagtcgat
     3781 cgaagcgtcg cggaaaacag aatcggtgcg aactacggtc cggatatcgc accacgtgtt
     3841 ttcaataccc tgccggttta aatattccga aacgatgacg ctggagatca actcgccgaa
     3901 aacgatcatc cggtcataaa tctcagccgc agtgccggtc acttcgctcc cggtaatcct
     3961 ttccagctca ttccattgcg ccccggtacg gatctcgggt tccttggagt catttgggaa
//
LOCUS       DAOQXS010000277         4005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23000_length_4005_cov_3.112658, whole genome shotgun sequence.
ACCESSION   DAOQXS010000277 DAOQXS010000000
VERSION     DAOQXS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23000_length_4005_cov_3.112658"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PKM34_08150"
     CDS             <1..1591
                     /locus_tag="PKM34_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNQ83600.1"
                     /translation="FFLVKFSGSLWMVYRLKNKSLYAVPEYWESRLRVLAQSIGVKGK
                     IKLAESALAHIPITIGYLKPVILVPVGTISGVPPQQLEAILLHEIAHIYRKDYILNIL
                     QCIVEMLFFYHPVVWWLSGLIRDEREHICDDIALSINNDQLNYIKALTLMEEMNVKSP
                     VIASAFTGSRKKLLGRVKRLIKPTRAKINPGAGITATMLLISLVFVISLTAFSFFPDS
                     YDLTGRESGERFYNILPSGVLKSTSSMSEEIKPVPAGAIPETAAGEPDTIIATSKSGK
                     VTIKVYTDTVDNDDQREIKVFVEALEEQLNEFDNQKKEIEEQVIVLKSMKDQVDSITK
                     TIVIRSGDSIRVITRDSTFILPEGFDTTFSSGEGFQLFGFEVPDIPEFPESPEMPDLR
                     YYYFDDQQIEAGRQFERALREQERKMKDFEWQQRKMERTNPDMPIIIDDPSRINHEWK
                     WVEGAPVPEMNPVEKIIRQELRDDGLISGWKKYVVEIGPKAMYLNGEKQPKEIFRKYR
                     KLVESLDQNVQENNSTFKIIF"
     gene            complement(1667..1984)
                     /locus_tag="PKM34_08155"
     CDS             complement(1667..1984)
                     /locus_tag="PKM34_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNQ83601.1"
                     /translation="MSDNKPKNQINIELGEDQAEGTYVNLAMISHSHSEFIVDFIKIM
                     PGMPKAKVKSRVILTPQHAKRLLRALKENIGKYEATFGQIETPEGSDGMPPFHLGGPT
                     AQA"
     gene            complement(2003..>4005)
                     /locus_tag="PKM34_08160"
     CDS             complement(2003..>4005)
                     /locus_tag="PKM34_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNQ83602.1"
                     /translation="NFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQ
                     DVIISEDDCGTLRGITIAALKKNEEIVESLYDRILGRVTLHDIFHPNSGELIAYAGQE
                     LTEEKCKMIEDSPIEAVEIRSVLTCESKQGVCARCYGRNLATGRMVQEGEAVGVIAAQ
                     SIGEPGTQLTLRTFHVGGTASNVAIISKIEAKYDGILEIDELRFVPFTNREGKSFDVV
                     IGRSAEMRIVDKHTRIVLASGHIPYGANLYFKENTEVRKGNLISDWDPFNAVILAEVK
                     GSIQYENIVEGKTYRIESDEQTGYVDKVVIEPRQKLKNPTINIISGDGEILKAYDIPV
                     GARIVVEDGQEIKSGEPLVKIPRAIGKTGDITGGLPRVTELFEARNPSEPAVVAEIDG
                     VVTINKKLKRGNREVIITSKTGEEKHYLIPTTKQILAQENDYIRAGTPLSEGEISPSD
                     ILAIKGPMKVQEYIVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVEVDDPGDTRFLEG
                     ELVNKTDFQDENDDVFGKKVVVDSGDSLSYRAGQIVSARKLRDENSMLKRKDKRLIEA
                     RDARPATGRQVIQGITRASLQTNSWISAASFQETTKVLTQAAIQAKSDELKGLKENVI
                     VGHKIPAGTGLRKYDDIIVGSREEYEEIMASKENFDDDMDVK"
BASE COUNT         1027 a          946 c          928 g         1104 t
ORIGIN      
        1 attcttcctg gtgaaatttt ccggatcctt gtggatggtt taccgactta aaaacaagtc
       61 tttatatgcc gttcctgagt attgggaatc caggctccgg gtattagcac aatcgatagg
      121 agtgaagggt aagattaaac tggctgaatc agccctggcc catatcccga tcaccatcgg
      181 ttaccttaag ccagttatcc tggtcccggt tggcacaata tcaggtgttc cgccacagca
      241 acttgaagct attttgctgc atgaaattgc ccatatatac cgtaaagatt atattttgaa
      301 tattttacag tgtattgtcg aaatgttgtt cttctatcac ccggtagtat ggtggttatc
      361 cggtttgata agagacgaaa gagaacatat ttgtgatgat atcgccctga gtatcaataa
      421 tgatcagtta aattatatca aagcgttaac tcttatggaa gaaatgaatg tcaaatcgcc
      481 ggtgatcgcc agtgccttta ccggtagccg aaaaaaacta ctgggccgtg ttaaaaggct
      541 gattaaacca acgcgtgcga aaataaaccc gggtgcaggg atcacagcca ctatgctgct
      601 gatcagcctt gtattcgtta tttccctcac agccttttca ttttttcctg attcctacga
      661 cctgacgggt cgcgaatcag gtgaacgatt ttataacatt ttgccgtcag gcgtgttgaa
      721 aagtacttct tcaatgtcag aggaaataaa accggttccg gccggggcga tccctgagac
      781 cgcagcagga gagccggata cgattattgc tacttcgaaa tcaggtaaag ttactataaa
      841 ggtatatacc gataccgtcg acaacgatga tcaacgggaa ataaaggtct ttgtcgaagc
      901 actggaagag cagttaaacg aattcgataa ccagaaaaaa gaaattgaag agcaggtgat
      961 cgtcctgaaa agtatgaagg atcaggttga cagcattacc aagacgattg tgatccgctc
     1021 aggcgattcg atcagggtga ttacccggga ttccacattc attttaccag aaggtttcga
     1081 tactactttt tcctccgggg aaggatttca gttattcggt tttgaagtac ctgacatccc
     1141 tgagtttccg gagtcgccgg agatgcccga cctgcgctac tattattttg atgatcagca
     1201 gattgaagct gggcgccagt ttgaaagagc attaagggaa caggaacgga aaatgaagga
     1261 ttttgaatgg cagcaacgta aaatggaacg gacaaatccc gatatgccca taataattga
     1321 tgatccgtca cgtataaacc acgaatggaa atgggtggaa ggcgcgccgg ttccggaaat
     1381 gaacccggtt gaaaagatta tccgtcagga gctaagggat gatggcctga tctccggctg
     1441 gaagaaatac gttgttgaaa tagggcccaa agccatgtat ctaaatggcg aaaagcaacc
     1501 gaaagaaatt ttcagaaaat accggaagct ggtcgagagt cttgaccaga atgttcagga
     1561 gaataacagc acgtttaaaa taatattcta aggtctttgg aaccgacggc tttagccgtc
     1621 ggaagaaaaa agaagaaccg acggctaaag ccgtcggttc ttctttttat gcctgagcag
     1681 ttggtccccc caggtggaag gggggcatgc catcagatcc ttccggcgtt tcgatttgtc
     1741 cgaaggtcgc ctcgtatttt ccaatattct ccttcagtgc ccgtaacagc cttttggcat
     1801 gctggggtgt caggatcact ctggatttta ctttggcttt gggcattccg ggcatgatct
     1861 tgataaaatc gacaataaac tccgagtggg aatgggagat catagccagg ttcacataag
     1921 tgccttcggc ctggtcttcg cccagttcga tattgatctg atttttaggc ttgttatctg
     1981 acatactctt caatattaag cattatttaa catccatatc atcgtcgaag ttctccttgc
     2041 tggccatgat ctcttcatat tcttcacggg agccgacaat gatatcgtca tattttcgga
     2101 gaccggtacc ggcgggaatc ttatgcccga caataacatt ctctttcagg cctttgagtt
     2161 catccgattt agcctggata gccgcctgcg ttaaaacttt ggtggtttcc tggaatgatg
     2221 cagcggaaat ccagctattg gtttgcagag aagctctggt aatcccctgg atcacctggc
     2281 gtccggtagc tggacgggca tcgcgcgcct cgatcaaccg cttgtccttc cgtttcagca
     2341 tggaattctc atcccttaat ttacgggcgc tgacaatctg gccggccctg taagagagcg
     2401 aatcaccgga atcaaccaca accttttttc cgaaaacatc atcgttttca tcctggaaat
     2461 cggttttgtt aaccagttcc ccttcaagga accgggtatc accgggatca tccacttcta
     2521 ctttgcgcat catctgacgg acaatgacct caaagtgttt atcattgatc ttcacaccct
     2581 ggagacggta aacttcctgt acctcattta caatatattc ctggaccttc atagggcctt
     2641 taatagcgag gatatcggaa ggagaaattt ctccttctga cagaggcgta ccggcgcgga
     2701 tatagtcatt ttcctgtgcc aggatctgtt ttgtcgttgg gatcaggtaa tgcttttctt
     2761 cccctgtttt agaggtgatg ataacttcac ggttacccct tttcagcttc ttgttgatcg
     2821 taacaacacc atcaatctcg gctacaacag ccggttcgga aggattcctg gcctcgaaaa
     2881 gctcggttac gcgcggaaga cctcccgtga tgtctccggt cttgccgatg gctctcggaa
     2941 tcttcaccag cggctcgccc gatttgattt cctgcccgtc ttccactaca atacgtgcgc
     3001 cgaccgggat atcataagcc tttaatattt ctccgtcgcc ggatatgata ttaatggtag
     3061 gattcttcag cttttgccgg ggttcaatga caaccttatc gacataaccg gtctgttcgt
     3121 ccgattcgat acggtaagtc tttccttcaa cgatattttc atactggatc gaacccttaa
     3181 cttcggcgag aattactgcg ttgaatggat cccagtcgct gatcaggttg ccttttctga
     3241 cttccgtatt ctctttgaaa tataagttag cgccatacgg aatatgccct gatgccaata
     3301 cgatacgggt atgtttgtcg acaatcctca tttcagccga cctgccgatc acaacatcga
     3361 atgatttgcc ctcacggttg gtgaaaggga cgaaccggag ctcatcgatt tcaaggatac
     3421 cgtcgtattt ggcttcaatc tttgaaataa tagctacgtt cgatgcagta cccccaacgt
     3481 ggaaagtacg aagtgttagc tgggtacccg gttccccgat ggattgggct gcgatgacgc
     3541 caaccgcctc tccttcctga accatccggc cagtcgccag gtttcgtccg taacaccgtg
     3601 cacaaacccc ttgttttgac tcacaagtca gtaccgaacg gatttcaacg gcttctatcg
     3661 gcgaatcttc aatcatcttg cacttttctt cagtcagttc ctgtccggca taggcaatca
     3721 attcacctga attcggatgg aagatgtcat gaagggtaac acgtcccagg atcctgtcgt
     3781 agagtgattc cacgatttct tcatttttct tgagagcagc aatggttata cctctaagag
     3841 tgccgcagtc gtcctccgaa atgatcacat cctgggccac atcgaccaga cgacgggtta
     3901 aatatcccgc atctgcagtc ttaagagcgg tatcggccag acctttacgg gcgccgtggg
     3961 ttgaaatgaa gtactccaga accgacaaac cctctttaaa gttgg
//
LOCUS       DAOQXS010000278         3995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23088_length_3995_cov_2.944670, whole genome shotgun sequence.
ACCESSION   DAOQXS010000278 DAOQXS010000000
VERSION     DAOQXS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23088_length_3995_cov_2.944670"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="mutL"
                     /locus_tag="PKM34_08165"
     CDS             complement(<1..948)
                     /gene="mutL"
                     /locus_tag="PKM34_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNQ83603.1"
                     /translation="MADIIQLLPDSVANQIAAGEVILRPASAVKELLENAIDAGASEI
                     NLIIKDAGKTLIQIVDNGSGMSERDARMSFERHATSKIKQASDLFAIRTLGFRGEALA
                     SIAAIAQIEMRTRRHEDELGTLLEIEGSRLKNQSDCSCPAGTSIMVKNLFFNVPARRS
                     FLKTETAELKHIVDEFFRVALVYNEVRFSLHHNQKMLYQLLPGNRKQRIVSLLGAGYN
                     ERLVPVEHITDAITITGFIGKPEFARKSRGEQFFFVNGRYIRHSYLSHSVENAYRELI
                     PVDSLPSYFIYINTDPGTIDINIHPTKTEVNFLDSKVIYS"
     gene            complement(952..1254)
                     /locus_tag="PKM34_08170"
     CDS             complement(952..1254)
                     /locus_tag="PKM34_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83604.1"
                     /translation="MSFFFFRKPKPRQFNYKPLYYDPDTEEAMERKKILEGLQSSDPG
                     ERLRAQIRRRWRKESAQADSRPQVIRFAFYVIIAAFAIYFIFFTDIINKIVTLFVR"
     gene            complement(1319..1876)
                     /locus_tag="PKM34_08175"
     CDS             complement(1319..1876)
                     /locus_tag="PKM34_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83605.1"
                     /translation="MSKLKLHSGLIFIISIIFTGALFRFIPHWPNFTPIAAMALFGGA
                     YLGRKHLAFLLPFAAMFLSDLILGLHKDMWAVYVAFGMTVTIGIAIRNRIGFTSVITA
                     SVVSSVLFFMVTNFAAWLSSPFYPQTFAGLMQSYVAGLAFFNNGSLGLSFFLNELTGS
                     LFYNGLFFGAYAGVKYWKPQLVKVS"
     gene            1994..3637
                     /locus_tag="PKM34_08180"
     CDS             1994..3637
                     /locus_tag="PKM34_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ83606.1"
                     /translation="MMKLSSILATLVIMVILNSCISKEKADLILFNGKIYTTDSAFSI
                     KEALAVSNGNFIATGTNEEILSAYSSEIIVDAGGKVILPGLIDGHCHFFGYAQNRYQG
                     VNLRGTSSFEEIVDKLKDFHSQYPEKWITGTGWDQNDWNDKAFPDNLILNTLFPDIPV
                     ILTRIDGHAVLANSYALQLARIDSKTVIPGGKVLLKDGKPTGMLLDNAADSMKKLIPA
                     PDQQSRALALQSAQEDCFRYGLTSVVDAGLDYNEIKLIDSLQNSGHLKIRINAMLSPT
                     DKNIREFLGKGSFRKERLTVNSLKLYADGALGSRGALLLEPYADDPGNYGVMVADESH
                     YRVLLSMAYNSGFQVNTHCIGDSANRMILNLYREVLNGPNDRRWRIEHAQVVNPGDFR
                     LFGDYRIIPSVQATHCTSDMYWAGERLGPDRIKYAYAYKKLLDENGWIINGTDFPIEE
                     VNPMLTFFASTGRMDKSGYPENGFQTENALSREETLRSMTIWAAKGSFEDDIKGSIEP
                     GKVADFVILDRDIMEVPFPEIPKTVVLKTYLNGELVFEN"
     gene            complement(3644..>3995)
                     /locus_tag="PKM34_08185"
     CDS             complement(3644..>3995)
                     /locus_tag="PKM34_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNQ83607.1"
                     /translation="GRLPYLAHGYMGRELLEKKGLKDIAPVCERHIGVGITIEEIKQN
                     NLPLPVRDMVPQTIEEKIICYADKFFSKSASNLSQPKPLDKVKRSIRKYGDTNWDVFE
                     EMMNLFGTESVYGK"
BASE COUNT         1170 a          895 c          904 g         1026 t
ORIGIN      
        1 ggaataaatg accttgctgt caagaaaatt cacctccgtc ttggttgggt gaatattgat
       61 atcaatggtt cccggatcgg tgttgatata gatgaagtag ctcggcaatg aatcgaccgg
      121 gatgagttca cggtaagcat tttcgaccga atggctcagg taggaatgcc ggatataccg
      181 tccgttgacg aaaaaaaact gttcgcctct gcttttgcgc gcaaactcgg gtttacctat
      241 aaatcccgta atggttatag catcggtaat atgttcaacg ggtaccagcc gctcattata
      301 accggctcca agcaacgaca cgatcctttg tttcctgttg ccgggcaaaa gctgataaag
      361 cattttttgg ttatgatgaa gggaaaatcg cacctcatta taaaccaatg cgacacggaa
      421 aaactcatcg acaatatgtt tcagttcggc ggtctcggtt tttaagaaac ttcttcgggc
      481 cggaacattg aaaaacaaat tcttgaccat gatggaggta ccggctggac aactgcaatc
      541 cgattggttt ttcagacgag aaccttcaat ttccaataag gttcccagtt catcctcatg
      601 acggcgtgtc cgcatctcta tctgggcaat cgcggcaatc gaagccaggg cttcaccccg
      661 gaatccgagt gtccggatgg caaacaggtc actggcttgc tttatcttgc tggtagcatg
      721 tcgttcgaat gacatcctgg catccctttc cgacatgcca ctgccattgt caacgatctg
      781 gatcaaggtt ttgccggcat ccttgataat gaggttaatt tccgaagcac cggcgtcaat
      841 ggcattttcc agcaattctt taactgctga agcaggcctg aggataactt caccggcagc
      901 aatctggttt gcgacggaat ccggaagcag ttgaatgata tcagccatta tttacctgac
      961 aaatagggtc acaatcttgt tgataatatc ggtaaagaag ataaaataaa tggcaaaagc
     1021 tgcaatgata acataaaatg caaagcggat cacctgtggc ctggaatcgg cttgagcact
     1081 ttcctttcgc catcttcgcc tgatctgcgc tctcaacctt tctcctggat ccgaactctg
     1141 aagcccttcc aggatttttt ttcgctccat agcctcttcc gtatcaggat catagtataa
     1201 aggcttataa ttgaactgcc tgggtttcgg tttcctgaaa aagaaaaaac tcatccttta
     1261 agtttttaac aaaaataaag aaaaacgtgg ctaaaaaatc cacgggcgac acaggtcgtt
     1321 aagaaacctt cacaagctgt ggtttccagt attttacccc tgcataggcg ccaaaaaaca
     1381 atccgttata aaacaacgac ccagtgagtt cattgaggaa gaaagacaaa cctaacgatc
     1441 cgttattaaa aaatgcaagc ccggcaacat aagattgcat caatccagca aaagtctgcg
     1501 gatagaaggg gcttgacagc catgccgcaa agttcgtcac catgaagaaa agcacggaag
     1561 atactaccga agctgtaatg accgaagtaa atccgatccg gttacggata gcgattccaa
     1621 tagttactgt catgccaaaa gcaacataga ctgcccacat atccttatgc aatcccagga
     1681 taagatcgct gagaaacata gcggcaaaag gcagaaggaa ggctaaatgt ttccttccca
     1741 ggtatgcgcc tccaaacaat gccatagcag ctatgggtgt aaagttcggc caatgaggga
     1801 taaaacgaaa caatgcaccg gtaaaaatga tggaaataat aaatatcagg ccagaatgta
     1861 atttcagttt gctcatgttc ccagttttaa atcggctaca aatataagaa ggatttacca
     1921 tctcctccgg tcaaatcgag atttatatta acaggctttt cttaattttg ttcgcaacgt
     1981 gaaagactta aatatgatga aattatcttc aatcctcgca actctggtca ttatggtgat
     2041 tcttaacagc tgcatatcca aagagaaagc cgatcttata ctattcaacg gaaaaatata
     2101 tacgaccgat tccgcattca gcataaaaga ggccctggca gtcagcaacg ggaatttcat
     2161 tgctaccggg acaaatgaag aaatcctctc agcctattct tcggaaataa ttgtagatgc
     2221 aggagggaag gtgattcttc ccgggctgat cgacggtcac tgccatttct tcggatatgc
     2281 ccagaaccgt taccagggcg taaatctcag aggaacatca tctttcgaag aaatagtgga
     2341 taaactgaaa gactttcatt cccagtaccc ggaaaaatgg atcacaggaa ccggttggga
     2401 ccagaacgac tggaacgaca aagcttttcc ggataatttg atactcaaca ctctttttcc
     2461 ggatattccc gtcatcctga cccggattga cggtcatgcc gttctggcca actcctatgc
     2521 tcttcaactg gcccgaatcg acagtaaaac tgtaatcccg ggcggaaaag ttttgttgaa
     2581 agatggtaaa ccgacaggta tgctgctcga taatgctgcc gattcaatga aaaaactcat
     2641 tccagcgccg gatcaacagt cgcgcgccct ggcattgcaa agcgctcagg aagattgttt
     2701 caggtatggt ctcacatcgg ttgtggatgc agggctggat tacaatgaaa taaagctgat
     2761 agattccctg caaaacagcg gacatttgaa aatcaggatt aacgccatgc taagccctac
     2821 ggataaaaat atccgtgaat ttctcggtaa aggatcattc cggaaagaga gattaacggt
     2881 taattcattg aaattgtatg ccgatggtgc gttgggatcg cgcggcgcat tattgctcga
     2941 gccctatgca gatgatcccg gaaattacgg ggttatggta gccgatgaaa gtcattacag
     3001 ggtactcctg tcgatggcat acaatagcgg attccaggta aatacgcatt gcatcgggga
     3061 ttcagctaac cggatgattt taaaccttta ccgggaagta ctaaatggcc cgaacgacag
     3121 gcgctggcgc attgaacacg cacaggtcgt caatcccggg gatttccgtc tttttggcga
     3181 ttaccggatc attccttccg tgcaggccac gcattgcacc agcgatatgt actgggcagg
     3241 agagaggctg gggcctgaca ggataaaata tgcctatgcc tacaaaaagc tgctggatga
     3301 aaacggatgg atcattaacg gcaccgattt tccgatcgaa gaggtcaacc cgatgctgac
     3361 attttttgca tccacgggta gaatggataa atccggttac ccggaaaatg gttttcagac
     3421 agaaaatgcc ctcagcaggg aagaaacgct acgaagtatg acgatctggg cagcaaaggg
     3481 aagttttgaa gacgatatca aaggcagcat tgagccggga aaagtagctg atttcgtgat
     3541 tcttgacagg gatattatgg aagtgccgtt tccggaaatt ccaaaaacag tggttcttaa
     3601 aacttatctt aacggggagc tggtctttga aaattgaccg aaattatttt ccgtatacag
     3661 attcggtccc gaacaaattc atcatctctt caaatacatc ccaatttgta tcgccgtact
     3721 tcctgatgct ccttttaacc ttatcaagag gtttaggttg agaaaggtta cttgctgatt
     3781 ttgagaagaa tttatcagca tagcagatga tcttttcctc gatggtctga ggaaccatat
     3841 ccctgacagg caatggcaag ttattttgtt tgatctcttc aatggtgatc ccgacaccga
     3901 tatgcctttc gcataccggg gcaatatctt tcaagccttt tttctccagc aattcccttc
     3961 ccatgtatcc atgggcaaga tagggcaggc ggccg
//
LOCUS       DAOQXS010000279         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23096_length_3994_cov_3.561056, whole genome shotgun sequence.
ACCESSION   DAOQXS010000279 DAOQXS010000000
VERSION     DAOQXS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23096_length_3994_cov_3.561056"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKM34_08190"
     CDS             complement(<1..929)
                     /locus_tag="PKM34_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNQ83608.1"
                     /translation="MKTLKYLLLLILFLSIGRQVSAAYLLIPMDDTQRNHLKAYGIAY
                     WVLTKDVEIYWLLNYRGGSYMFPHNKSFEDECIIRGVSYELIADVQSTAILEEIADPQ
                     VNMDAVKLHKAPKIAVYSPKNKLPWDDAVTLVLSYSEIPYDVIYDKEVIEGILPMYDW
                     LHLHHEDFTGQYGKFWSAYRSFPWYQEDVRINEETARALGFQKVSECKLAVAQKIRDY
                     VIGGGYLFAMCSAPDSYDVALSAEGVDICDIVFDGDPMDPDAQAKIHYDNCLAFTDFT
                     LSTNPYEYEISSIDVTETRPKNIEGKDFFTLFD"
     gene            complement(972..1847)
                     /locus_tag="PKM34_08195"
     CDS             complement(972..1847)
                     /locus_tag="PKM34_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ83609.1"
                     /translation="MTKQNKAYLLAGTAVFMWSTVGTAFKLTLSSLHPVIMLLYASLV
                     SIIVLFTILLIQGKLSLLLQVSRKDLLRSALNALLNPLLFYVVLFFSYDMLLTQEAMV
                     INFTWPLPLTLLSILILKQNISLKSVLAILISFLGIVVIATKGDILALHFENPAGVLL
                     ALSSTIIWSFFWIVNIRDKRDEVIKLFMNFSFGSIYVLLVAVFFKLPFTLSFNALLGI
                     GYIGIFEMGVAYVLWLKGLQLSETTAKVSNLLFIAPFFSLIFINIFVGERILWSTLAG
                     LAFVVTGIIMQRHIK"
     gene            1947..2891
                     /gene="arcC"
                     /locus_tag="PKM34_08200"
     CDS             1947..2891
                     /gene="arcC"
                     /locus_tag="PKM34_08200"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNQ83610.1"
                     /translation="MKKLGVVAFGGNALILGKQKGTIEEQEANAYQTCERLLVLIDNG
                     YDLVITHGNGPQVGNIMLANAAGEKLYGVPEMPLDISVAYSQGFIGYVIEQQLRNVLR
                     ENDIDRDIITIITQVLVNKDDPAFQNPVKPIGPFYTKEEAEKIRQASETIFAEDPRGR
                     GFRKVVASPRPLVIYNTKTIERIAREGQIVIAVGGGGIPAYYKTEYKLQGIDAVIDKD
                     HASALLASQIGAYRFFILTDVPKVCLNFNTPQEKTISRMNIAEAKRYLAEGHFSEGSM
                     APKIRAAIYFVEHSGRDAIITQTTLLGVDDGGTRITIV"
     gene            2990..3991
                     /gene="argF"
                     /locus_tag="PKM34_08205"
     CDS             2990..3991
                     /gene="argF"
                     /locus_tag="PKM34_08205"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ83611.1"
                     /translation="MSFNLRNRSFLKLLDFTPQEIRFLLDLSAELKRSKYAGSEQQRL
                     KGKNIVLLFEKDSTRTRCAFEVAAFDQGANVTYLGPSGSQMKKKESIKDTARVLGRMY
                     DGIEYRGYGQDIVEELAKYSNVPVWNGLTNEFHPTQVLADLLTMMEHSNKPLKEIAYC
                     YLGDARNNMGNSLLVGAAKMGMDFRSIAPKEFQPAKELVDKCLEIAKETGARITVTDN
                     VAEGVKGVDFVYTDVWVSMGEPDSAWKERLALMLPYQVNSQLMAKTGNPDVKFLHCLP
                     SFHSRETTVGEEIFQKFGVAEMEVTDEVFESPASIVFDQAENRLHTIKAVMVATLGS"
BASE COUNT         1189 a          886 c          971 g          948 t
ORIGIN      
        1 aaatcaaata aggtgaaaaa gtcttttcct tcaatatttt tgggccgggt ctctgtcaca
       61 tcaattgaag aaatttcata ctcgtacgga ttcgtactca atgtgaaatc ggtaaaagca
      121 agacagttat cgtaatggat ttttgcctgg gcatccgggt ccatcggatc accgtcaaaa
      181 actatgtcgc aaatgtcaac gccttcggcg gataaggcca catcataact gtcgggggcc
      241 gagcacatgg caaacaggta accgcctccg ataacgtaat cccttatttt ttgcgctacc
      301 gccagtttgc actcggaaac cttctggaaa cccagagcac gggccgtctc ttcatttatt
      361 cttacatctt cctggtacca ggggaaactg cggtatgccg accagaattt gccatactgc
      421 cccgtgaaat cttcgtggtg aagatgcagc cagtcgtaca tcggcaaaat gccttcaatc
      481 acttctttgt catagatcac atcgtatggg atctccgaat aggataacac cagcgttacg
      541 gcatcatccc acggaagctt gttctttggc gaatacacgg cgattttagg agccttgtga
      601 agctttaccg catccatgtt gacctgtgga tcggcgattt cctcaagaat ggctgtgctt
      661 tgaacatcgg cgatcagttc atagctgacg ccgcggataa tgcattcatc ctcaaaggat
      721 ttattgtgcg gaaacatata actgcccccg cggtaattaa gcagccagta tatctcgaca
      781 tctttggtca gtacccagta ggcaatgccg taggctttca aatggttgcg ctgggtgtcg
      841 tccatcggga tcagcaagta ggcagcagaa acctgccggc cgatcgaaag aaaaaggata
      901 agaagcaaca ggtattttaa tgttttcata actaacaaac ggatacaaaa gatttttgtt
      961 ttacaggaca atcacttgat gtgccgctgc atgatgattc ccgtgaccac aaatgccaga
     1021 ccggcaaggg tagaccagag aatcctttca cctacaaaaa tattgataaa aatcagggag
     1081 aaaaaaggag cgataaataa aaggttgctg actttggctg ttgtttcaga gagttgaagc
     1141 cctttcagcc ataaaacata agcaacaccc atttcgaaaa tcccgatata accaatgccc
     1201 agcagagcat tgaatgacag ggtgaacggc aatttgaaaa agacggcaac cagcaggaca
     1261 tagatagatc caaaagagaa gttcatgaat aattttatca cctcatcccg tttatcgcgg
     1321 atattcacaa tccagaaaaa agaccagata attgtgctgc taagagccag caatacgccg
     1381 gccggatttt caaagtgaag agcgagaata tccccttttg tcgcgataac aacaatcccc
     1441 aggaagctga tgaggatggc cagtacactt ttaagactga tattttgctt caggattagg
     1501 attgataata aggtaagagg cagcggccag gtgaagttga ttaccatggc ctcctgggta
     1561 agcaacatat cgtaggagaa gaaaagaaca acataaaaca gcagcggatt gagcaaggca
     1621 ttcagggcgg aacggagcag gtctttgcgt gaaacctgga ggagaaggct caattttccc
     1681 tggatcagaa ggatggtaaa cagaacgatt atcgaaacca gcgaggcata cagaagcata
     1741 attaccggat gcagcgacga cagggtcagc ttaaaggcag tgcccactgt cgaccacatg
     1801 aatacggccg tgccagccaa tagatatgct ttgttctgct ttgtcatccg gcgtattaga
     1861 acaggagcga agataaatat ttagctgaaa gatacagaat gagcctccaa atattattag
     1921 ctttgcagcc ttaaacggta ttttatatga aaaagcttgg tgtggttgct ttcgggggaa
     1981 atgccctgat tttgggaaag cagaaaggaa ctattgaaga acaggaagcc aatgcatatc
     2041 aaacctgtga acgcttgctg gtacttatcg ataacgggta tgacctggtg attacccatg
     2101 gcaacgggcc gcaggtaggc aatattatgc tggccaatgc tgccggtgag aaactgtacg
     2161 gtgttcctga aatgcctctg gatatcagtg ttgcctattc ccagggcttt atcgggtatg
     2221 taatcgaaca acagctcagg aatgtattgc gcgaaaacga tatcgaccgg gatatcatta
     2281 ctattatcac acaagttctt gtaaataaag atgatccggc ctttcagaat ccggttaagc
     2341 cgatcgggcc attctataca aaggaggaag cggagaagat cagacaggct tccgaaacca
     2401 tttttgccga ggatccgagg ggcaggggat tccggaaagt ggttgcttca cccaggcccc
     2461 ttgtgattta caatacgaaa accatcgaac ggattgccag ggaaggccag atcgttattg
     2521 ccgtcggagg tgggggaata cctgcctatt acaagacaga atacaagctt cagggcatcg
     2581 atgccgttat cgataaagat cacgcatccg cactgctggc ttcgcaaatt ggcgcttacc
     2641 ggttctttat cctgaccgat gtacccaaag tctgccttaa tttcaataca ccccaggaaa
     2701 aaacgatcag ccggatgaac attgcagaag cgaaacgtta cctggcggaa gggcattttt
     2761 cggaaggcag catggcgccg aagatcaggg cagccatcta ttttgtcgaa cacagcggcc
     2821 gcgatgccat catcacccaa accactctcc tgggtgtcga tgacggcggc acaaggatta
     2881 caattgtcta ataaacaagt gattagatac gatttccgat tgaaaattaa tattgaaaat
     2941 tgaaaattaa gaatttagaa ttaaaaattt agaactaaaa ttatatcgta tgtcattcaa
     3001 tttgcgcaac aggagctttt taaagctatt ggattttact cctcaggaaa tcaggttttt
     3061 attggatcta tcggcagaac tgaaacggtc aaaatatgcc gggagcgagc aacaaaggct
     3121 gaaaggtaaa aacattgtcc tgctttttga aaaagactcc acacgcaccc gctgtgcctt
     3181 cgaagtggcc gcatttgacc agggagcgaa tgtgacttat ctcggcccat ccggttcaca
     3241 gatgaagaaa aaagagtcga tcaaggatac cgccagggta ctcggtcgca tgtacgacgg
     3301 aattgaatac cggggatacg gccaggatat cgtcgaggaa ctggcaaaat attccaacgt
     3361 cccggtgtgg aacggcctga ccaatgaatt ccatcctaca caggtacttg ccgatttgtt
     3421 gacgatgatg gagcacagca ataagccatt gaaagagatc gcttattgtt acctcggcga
     3481 tgccaggaat aatatgggca attcgctcct ggtcggcgca gctaaaatgg gtatggattt
     3541 ccgttccata gcacccaaag agttccagcc ggccaaagag ctagtcgata aatgcctgga
     3601 aatagccaaa gagaccggcg cccgcatcac ggtgaccgat aacgtggccg aaggggtaaa
     3661 aggggtcgat tttgtttata ctgatgtctg ggtttcgatg ggcgaacccg attctgcctg
     3721 gaaagaaaga ctggcactca tgctgcctta ccaggtgaac agtcagctta tggctaaaac
     3781 aggcaatcct gatgtcaagt ttttgcactg cctgccctct ttccatagcc gtgaaactac
     3841 agtaggagag gagatattcc agaaattcgg cgttgctgaa atggaagtga ccgacgaagt
     3901 cttcgaatca ccggcatcca tagtgtttga ccaggccgaa aaccgcctgc ataccatcaa
     3961 agccgtaatg gtggctacgc tgggaagtta aaca
//
LOCUS       DAOQXS010000280         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23107_length_3993_cov_3.373540, whole genome shotgun sequence.
ACCESSION   DAOQXS010000280 DAOQXS010000000
VERSION     DAOQXS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23107_length_3993_cov_3.373540"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..474
                     /locus_tag="PKM34_08210"
     CDS             49..474
                     /locus_tag="PKM34_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNQ83612.1"
                     /translation="MTNDLRLMTNDPKSPGLTELVLDLMEQLKSYVNMRIDYVKLQVA
                     DYLIRFFSSLTLFIILFWIVFFAVFFGSFAFAYWFGEKTGMWSVGFLIVAGFYILLAV
                     LVYVFRRVLIIRPFTRLILEQLELNKFIEKENGKEERVP"
     gene            449..691
                     /locus_tag="PKM34_08215"
     CDS             449..691
                     /locus_tag="PKM34_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83613.1"
                     /translation="MEKKNEYRDIERLKAFQLGRIRQQEQAIKLSFKEVRDNITGEAL
                     KEKLKENLFSGPGLAFKLGYITVTLLQERFSKTKKK"
     gene            702..1706
                     /locus_tag="PKM34_08220"
     CDS             702..1706
                     /locus_tag="PKM34_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNQ83614.1"
                     /translation="MVSRFSDTFSLLSRITPRRMVNLLLLYFSYYAARTTGKVRHRGK
                     PFTVSIEPTTSCNLRCPECPSGLRQFTRPTGALSLELYRDIIDQLSPGLFYLILYFQG
                     EPFLNPLFFKMVEYARRKRIYTATSTNAHFLNDNFARKTVESGLDRIIISLDGLDQES
                     YEKYRTGGSVEKVFEGTRNLVRWKKELKSRTPYIILQFIVFSTNEHQVGELKKVAREL
                     GVDKLELKTAQVYNYEDGNDLIPSNPDYARYKKENGKFKIDNPLNNHCLRMWRGCVIT
                     WDGLVVPCCFDKDGSHRMGDLKINSFDEIWRGKAYDDFRRKLFTARSEIDICKNCTEK
                     "
     gene            1834..2028
                     /locus_tag="PKM34_08225"
     CDS             1834..2028
                     /locus_tag="PKM34_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83615.1"
                     /translation="MVLRKWEPTALVVGRKTHQEPTASVVGRKTHQEPTALVVGRKTH
                     QEPTASVVGARTFWFYIKQS"
     gene            complement(2015..2578)
                     /gene="frr"
                     /locus_tag="PKM34_08230"
     CDS             complement(2015..2578)
                     /gene="frr"
                     /locus_tag="PKM34_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ83616.1"
                     /translation="MTDESELLLQETEDQMNKSVQFLEKELHKLRTGKATPQMLEGIR
                     INYYGTPTPIEQTANITTPDARQIIIQPWDKSIIHDIDKAIMAANIGLNPKNEGEVLR
                     IIVPPLTEERRRDLVKKAKTEAENARISVRNIRRSSNDMAKDLEKEGVPEDEVKKLQE
                     EIQKLTTRFIDLVDKIVVQKEKDIMTV"
     gene            complement(2609..3274)
                     /locus_tag="PKM34_08235"
     CDS             complement(2609..3274)
                     /locus_tag="PKM34_08235"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNQ83617.1"
                     /translation="MRLHRKGFLPLLVTLAILLALVWMINLAFPVQTCIHNILYGIGI
                     VFYFLCIRFFRLPSRTVDEKHENIISSADGHVVVIEEIDETVFFHDRRIQVSVFMSPL
                     NVHVNWYPISGEIVHTKYHRGSHIPAYRPKASLENERMSVVIRGEHGHEVMMTQIAGL
                     VARRVFTNAKKGSYVFQGEEAGIIRFGSRVDLILPVGTDIRVKIGQKVRACETVIGIF
                     PEK"
     gene            complement(3271..>3993)
                     /locus_tag="PKM34_08240"
     CDS             complement(3271..>3993)
                     /locus_tag="PKM34_08240"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNQ83618.1"
                     /translation="YILMIAGMLEYFRIVSSGDVFPQRYNGLAAGSIVFLLPSLASLG
                     WISPKFLVILPTLVMLFFAAELFRNKPNSLQNLTFTLLPVGFIAIPMATLFLLMSPLV
                     TEDQPHWHLLFGFFIISWSYDTFAYLTGIWLGKHKLFEKISPKKTWEGTGGGAIFGLV
                     AAMVMSVFFPVLTPAEWIAGGLIIMVTGTLGDLSESLLKRNCHVKDSGTFFPGHGGVL
                     DRFDSILFAAPAFFCYLILLNL"
BASE COUNT          955 a          994 c         1048 g          996 t
ORIGIN      
        1 ttatgatgga gcgattgaga gattgagcga aggagtgaat agtgaatagt gactaacgac
       61 ctacgcctaa tgaccaacga cccaaaatcc cccgggctga ctgaactggt gctcgatctg
      121 atggagcaac tgaagtctta tgttaacatg cggatcgatt acgtcaagct gcaggtagcc
      181 gattacctga tccgcttctt ttcgagcctg acgttgttta tcatcctctt ctggatcgtc
      241 tttttcgccg ttttcttcgg ttcgtttgcc tttgcctatt ggttcgggga gaaaaccggc
      301 atgtggtcgg tcgggtttct tatcgtcgcc ggtttttata ttctcctggc cgtactggtt
      361 tatgttttta gaagggtatt gattatcagg ccttttaccc ggcttattct ggaacagctt
      421 gagttgaata aattcattga aaaggaaaat ggaaaagaag aacgagtacc gtgatatcga
      481 acgcctgaaa gcttttcaac tcggccggat caggcagcag gaacaggcga tcaaactgag
      541 ctttaaagag gtgcgcgaca atatcaccgg cgaagcgctg aaagagaagc tgaaggagaa
      601 cctgttttcc ggcccgggac tggcattcaa gctgggctat atcaccgtaa cgctgctgca
      661 ggaacggttt tccaaaacga agaagaaata gtcgtccggt cttggtttcc cgtttttcag
      721 atacattctc gctcctttcc cggatcacgc cgcggaggat ggtcaacctg cttctgttgt
      781 atttttccta ttatgccgcc cggacgaccg ggaaagtccg tcatcgcggc aaaccgttta
      841 ccgtttccat tgaaccgacc acctcttgca atctccgctg ccccgaatgc ccgtcgggcc
      901 tgcggcagtt tacacgtccg acgggcgccc tgagcctgga gctgtaccgc gatattatcg
      961 atcagctctc tcccggactt ttttacctga tcctctattt ccagggggaa ccttttctga
     1021 atccgttgtt tttcaagatg gtagaatatg cccgcaggaa gcggatttac acagccactt
     1081 ccaccaatgc gcactttctg aacgacaatt ttgcccggaa aacggtcgaa tcgggcctcg
     1141 accggatcat catctcgctc gacgggctag accaggagag ctatgaaaag tacaggacag
     1201 gagggagcgt ggagaaagtt ttcgaaggga cgcgcaacct ggtgcgctgg aaaaaagagc
     1261 tcaaatcccg cacgccgtat atcatcctgc agttcattgt tttcagcacc aacgagcacc
     1321 aggtcggcga actgaagaaa gtcgccaggg aacttggagt cgacaagctg gagttgaaaa
     1381 ccgcgcaggt ttataattat gaagatggca acgacctgat cccttccaat ccggattatg
     1441 cgcggtataa aaaagaaaat gggaaattta agatcgataa tccgctgaat aaccattgcc
     1501 tgcgcatgtg gcgcggctgc gtaatcacct gggacgggct ggttgtgccc tgctgcttcg
     1561 acaaagacgg cagtcaccgc atgggagacc tgaagatcaa tagctttgat gaaatctgga
     1621 gaggaaaggc gtatgatgat tttcgaagga aattattcac cgcccgaagt gagattgata
     1681 tctgtaagaa ttgtactgag aaatgagttc ctttttgaag taaaagatgc cttaaaattc
     1741 tgagcagccc ctttttattg gtagtagggc tttttaaaaa gtacaaatat tcattttata
     1801 ttggtagtcc aacgaataaa accgttggtt cttatggttt tacgaaagtg ggaaccgacg
     1861 gctttagtcg tcggaaggaa aacccatcag gaaccgacgg cttcagtcgt cggaaggaaa
     1921 acccatcagg aaccgacggc tttagtcgtc ggaaggaaaa cccatcagga accgacggct
     1981 tcagtcgtcg gagcacgaac tttctggttc tatatcaaac agtcataatg tccttctcct
     2041 tctgaacaac gatcttatcc accaggtcga tgaatctggt cgtcagtttc tggatctctt
     2101 cctgcagttt tttcacctcg tcttccggca cgccttcttt ctcaaggtct ttcgccatgt
     2161 cgttcgaaga gcggcggatg ttgcgtacgc tgatcctggc gttctcggct tcggttttgg
     2221 cctttttgac caggtcgcgc cggcgttctt cggtcagtgg cgggacgatg atgcgcagca
     2281 cttcgccctc attcttcgga ttcaggccga tgtttgccgc cattatcgct ttgtcgatat
     2341 catggatgat gctcttgtcc catggctgga tgatgatctg gcgggcgtcg ggcgtggtga
     2401 tgttagccgt ctgctcgatc ggtgttggtg ttccgtaata attgatcctt atcccttcca
     2461 gcatctgggg cgtagctttg ccggtccgca gtttgtgaag ctctttttcc aggaactgga
     2521 ccgatttgtt catctggtct tcggtttcct gcaatagtaa ttcagattca tccgtcatga
     2581 ttatcgatat taatttttta caaaaatatt atttctccgg gaaaatgcca atgaccgttt
     2641 cacacgccct gactttctgc ccgattttca cccggatatc ggtaccgacc ggcaggatca
     2701 ggtcgacacg ggagccgaaa cggataatgc ccgcttcttc gccctggaaa acgtaactgc
     2761 ccttttttgc attggtgaac acgcgccggg ccaccagccc cgcgatttgg gtcatcatca
     2821 cctcgtgccc gtgctcgccg cggatcacca ccgacatccg ctcgttctcc aggctggctt
     2881 taggtcgata ggccgggata tgcgaaccgc ggtggtattt cgtatgcacg atctccccgc
     2941 tgatcgggta ccagttgacg tgaacgttca gcggcgacat aaacaccgat acctggatcc
     3001 ggcggtcgtg aaaaaagacc gtttcgtcga tctcctcgat gaccaccacg tgcccgtcgg
     3061 cgctgctgat gatgttttcg tgcttttcat ccaccgtgcg gctgggcaac cggaagaacc
     3121 ttatacacag gaagtaaaag acgatcccga tgccgtaaag gatgttatga atgcaggtct
     3181 ggaccggaaa agcaaggttt atcatccaga ccagcgccag gagtatggcg agagtcacca
     3241 gcaggggcag aaatcctttg cggtgcagtc tcataggttc agcaggatca ggtaacagaa
     3301 aaatgccgga gcggcaaaca ggatcgagtc aaaccggtcg agcaccccgc cgtgccccgg
     3361 gaaaaaggtg cctgagtctt tgacatggca gttgcgtttg agcagcgatt cggaaagatc
     3421 gcccagtgtc cccgtcacca tgatgatcag ccctccggct atccattccg ccggcgtcag
     3481 cacggggaaa aataccgaca tcaccatcgc ggcaaccagt ccgaatatgg ctcctcctcc
     3541 tgtcccttcc caggtttttt tcggcgaaat cttttcgaac aatttgtgtt tacccagcca
     3601 gatcccggtc aaataagcaa aagtgtcgta actccaggag atgatgaaga atccgaacag
     3661 gagatgccag tggggctggt cttccgttac cagcgggctc atcagcagga acagcgtagc
     3721 catcgggatg gcaataaaac cgacaggcag aagggtaaag gttaaatttt ggaggctgtt
     3781 gggtttgttc cggaataatt cggctgcaaa gaacagcatg accagggtag gaaggatgac
     3841 gaggaatttg ggcgatatcc atccgaggga agccagggag gggagaagga agacgatgct
     3901 gccggcggcc agcccgttgt atcgttgcgg aaagacatcg cctgaactca cgatccggaa
     3961 atactccagc atgccggcta tcatcaggat ata
//
LOCUS       DAOQXS010000281         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23127_length_3990_cov_3.046760, whole genome shotgun sequence.
ACCESSION   DAOQXS010000281 DAOQXS010000000
VERSION     DAOQXS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23127_length_3990_cov_3.046760"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKM34_08245"
     CDS             <1..372
                     /locus_tag="PKM34_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83619.1"
                     /translation="ARSLTFAEVSSSEEDSLSYAVAENFYMQGRCDQAIASFKKYLDS
                     FAAGMYTTHAKFYQAQCFLKSNQLDPALARFKEVGQGPRSQFTEPALATASSMEFTLG
                     NYGLPYRSSSNWRPMPEIPIM"
     gene            339..863
                     /locus_tag="PKM34_08250"
     CDS             339..863
                     /locus_tag="PKM34_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83620.1"
                     /translation="METDAGDPDNVAAAITGQMRCHFKLGNYPAAALAAQKIMATGTA
                     TPELTGEARYILTKGYLAQGNIQAAESELVILSKMTGTEYGAEASYHLAEIAFNSDRL
                     QDAETLVYALSENYTAYDYWVARGFILLADVFTKSGNVFQARQTLQSIIDNYQGPELG
                     EIAREKLKSLEIKE"
     gene            891..2588
                     /locus_tag="PKM34_08255"
     CDS             891..2588
                     /locus_tag="PKM34_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83621.1"
                     /translation="MTRIIILRAILLPVLAVIMLAAGMPLASGQGKNEEVTIIAPYIP
                     SIGDATKISFKPEIKPEDNDTAKFSFSYTDRPYPVHIDPQPLEAIKYGQGDKPQLYRN
                     FAKIGIGNYYTPYLDFMATSLQSEKLLFGARLKHHSSQGKIKGYPVSAYSHNLIDIFG
                     KTFFKSHTLSLEAGYDRDVVHFYGFQPDSFPAFEFTKENIRQRFQHLDAGIEFSSNYR
                     ENDKLNHSIRLGVDHFTDLYGSRETEVGFKTRFDKSFKAGRSDFNHAVALDFGLDFLN
                     FRDSISSSSPFFMQFKPVYRLHFGQYRFMAGIDVNLAATDSPGGSSVGIDVFPLLKAE
                     VIVIPSQLSAYAEISGERTINSFRNLAGINPFIISNPQVSFTDEQFSIGGGITGNAAG
                     INFHVSAAYSYINDMALFVTDTALVPENRFKVIYDDLNRVKIQAGLGYVKTGRFSARM
                     NAAYYYYKPENELKAWHLPDYEVDLDASYTFLERYTAKLSLLALGSKYAPVYASGETA
                     SEKLNGALDLGAGAEYEISHMLAAWLDVSNILNQHYQRWQEYPVQGILIMAGVKLSF"
     assembly_gap    2792..2891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3000..>3990
                     /locus_tag="PKM34_08260"
     CDS             3000..>3990
                     /locus_tag="PKM34_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83622.1"
                     /translation="MTAEEKILQAQEYTADNIQVLEGLEAVRKRPAMYIGDISQRGLH
                     HLIHEVVDNSIDEALGGYCDRIDLTIHEGNSITVLDNGRGIPTGLHEKENRSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSKKLVATVYREGKIFRQEYNFGKPVYD
                     VQVIGDSDKTGTQVTFTPDDSIFTVTEFDYDLISARLRELAFLNKGIRITLTDERQPN
                     EDGSFHSTSFYSEEGLKDFIKYLDVNREALMPDPITIEGERGAIPMEIALQYNTSFAE
                     NIHAYVNNINTHEGGTHLAGFRRGLTRTLKNYAEKAGLLAKLKFDINGDDFREG"
BASE COUNT         1026 a         1029 c          922 g          913 t
ORIGIN      
        1 gcccgcagcc tgacttttgc cgaggtcagt tcttccgaag aagattcgct cagctacgct
       61 gtagccgaga atttttatat gcagggacga tgcgaccagg ctatcgcgtc ctttaaaaaa
      121 tacctcgaca gctttgcagc agggatgtat accactcatg caaagtttta ccaggcccag
      181 tgcttcctga agtctaacca gcttgatccg gcgttggccc ggtttaaaga ggtgggacag
      241 ggaccgcggt cgcagttcac cgaaccggcc ctggccacag cttcctcaat ggaatttacg
      301 ctgggcaatt acgggctgcc ctaccgctct tcgagcaatt ggagaccgat gccggagatc
      361 ccgataatgt agcggcagcc atcacaggtc agatgcgttg ccatttcaaa ctcggaaatt
      421 atcccgctgc agccctggcc gctcagaaaa ttatggctac gggtacggca acacccgaac
      481 tcacgggcga agcccgctat atcctcacca aaggttatct cgcacagggt aatatccagg
      541 ccgccgaaag cgagctggtg atcctcagca aaatgacagg tacggaatat ggcgccgaag
      601 ccagctatca cctggccgag atcgccttta attcagaccg gttacaggat gcagaaacac
      661 tcgtttatgc cctgtcggaa aactatactg cctacgatta ctgggtagca cgcggattta
      721 tcctcctggc cgatgttttt accaaaagcg gtaatgtttt ccaggcccgg caaaccttgc
      781 agagcatcat cgataactac cagggtccgg aactgggcga aatcgccaga gaaaaactca
      841 aatccctcga aatcaaagaa taactttccc cgataataga attagaaact atgacgcgga
      901 tcataatatt aagagcaatt ctgctgccgg tactggcggt catcatgctg gctgccggta
      961 tgccactggc ttcagggcag ggaaagaatg aagaggtgac gatcatcgca ccttacatcc
     1021 catcgattgg cgatgcaacc aagatttctt tcaaacccga aataaaaccg gaagataacg
     1081 atacggctaa attcagcttt tcctataccg accggcctta tcctgttcat atcgacccgc
     1141 agccgctgga agcgatcaaa tacggccagg gcgacaaacc tcagctatat cgcaattttg
     1201 caaaaatcgg cattggtaat tactataccc catacctcga ttttatggct acctcgctgc
     1261 aatccgaaaa attgcttttc ggcgcacggc tcaaacacca ttcgtcgcag ggaaagatca
     1321 aaggttaccc cgtcagcgct tacagtcaca acctgattga tatttttgga aagacattct
     1381 ttaaatcgca caccctgtca ctggaggcgg gatacgacag ggatgtcgtc catttctacg
     1441 gtttccagcc cgacagtttt ccggcgttcg agttcacaaa ggagaatatc cggcagcggt
     1501 tccagcatct ggatgccggt atcgaattca gcagcaatta ccgggagaac gacaaactga
     1561 accattcgat ccggctggga gttgatcatt tcaccgacct ttacggctcg cgcgaaaccg
     1621 aagtgggatt taaaacccgc tttgataaat ccttcaaagc aggccgtagc gacttcaacc
     1681 atgcagtggc actggatttc gggctggatt ttctgaattt cagagactcc atctcctcct
     1741 cttccccttt ctttatgcaa ttcaaaccgg tttaccggtt gcattttggc caataccgct
     1801 ttatggccgg aattgatgta aacctggcgg ctaccgattc accgggcggt tcatcggttg
     1861 ggatcgacgt attcccgctc ctgaaggccg aggtcatcgt catcccttcg caactcagcg
     1921 cgtatgccga aatttcggga gaacgaacga tcaattcttt ccggaacctg gccggcatca
     1981 acccgtttat catctcaaac ccgcaggttt catttactga cgagcagttc tccattggcg
     2041 gcgggatcac cggcaatgcc gccggcatca atttccatgt atcggccgcc tattcttaca
     2101 tcaacgatat ggccctgttc gtcaccgata cagccctggt gcccgaaaac aggtttaaag
     2161 tcatttacga cgacctgaac cgggtgaaga tccaggctgg actgggctat gttaaaacag
     2221 gccggttttc ggcacggatg aatgcggctt actattatta taaacctgag aatgaattga
     2281 aagcatggca cctgcctgat tatgaagtgg atctggatgc atcgtacact ttcctggaaa
     2341 gatatacggc aaagctttct ttgctggccc tgggctcaaa atatgccccg gtttacgcat
     2401 ccggcgaaac cgcttcggaa aagctcaacg gcgcactcga tttaggcgcc ggcgccgaat
     2461 atgagatcag ccatatgctc gcggcctggc tcgatgtcag taacatcctg aaccagcatt
     2521 accagcgctg gcaggaatat cccgtccagg gcatccttat tatggccggg gttaaattat
     2581 ctttttaaag gtaggaaccg acggctttag tcgtcggact acctcatctc tcacgacggc
     2641 tttagtcgtc ggactacctc atctctcacc gacggattta cccgtcggac aaccgaaccc
     2701 atctagctat ccttccatcc cttcttaaaa aacgatcacc aaattgttaa taaataggtt
     2761 gattttactg atgttttaac aactttaatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn ncaccaaatt gttaataaat aggttgattt tactgatgtt ttaacaactt
     2941 taatccggat aaaatccgta aatttgtaac cttatctatt tatttgtaac taattgatta
     3001 tgactgcaga agaaaagatt ttacaggctc aggaatatac agcagacaac atacaggttt
     3061 tggagggcct ggaggccgta cggaaacgcc cggccatgta catcggggat atcagccagc
     3121 gcggattgca ccacctgatc catgaagtag tagacaactc gatcgacgaa gcccttggcg
     3181 ggtattgcga ccggatcgac ctgaccatac acgaaggcaa ctcgattacc gttcttgaca
     3241 atggacgtgg tatccctaca ggcctgcacg aaaaagagaa caggtcggca ctcgaagtgg
     3301 tcatgaccgt cctccatgcc ggtggaaaat tcgacaaagg atcttacaaa gtgtccggtg
     3361 gtcttcacgg cgtaggggta tcgtgcgtga acgccctttc aaaaaagctg gtagcaacgg
     3421 tttaccgcga aggaaaaata ttccgtcagg aatataactt cgggaaaccg gtttacgatg
     3481 tccaggtgat tggcgactcg gacaaaacag gcacacaggt cacttttacc cccgatgatt
     3541 cgatctttac cgtcaccgaa ttcgattacg acctgatctc agccaggctt cgcgaactgg
     3601 cctttctcaa caaagggatc cggatcacgc tgaccgatga gaggcaacct aatgaagacg
     3661 gcagtttcca ttcgacttct ttttattcgg aagaagggct taaggacttt atcaaatacc
     3721 tcgacgtgaa ccgtgaggcg ctgatgcccg atcccatcac gatagagggg gaacggggcg
     3781 ccatcccaat ggaaatcgca ctccaataca atacctcatt tgccgagaac atccacgcgt
     3841 acgtcaataa catcaacacc cacgaaggcg gaacgcacct ggcgggtttc cggagaggac
     3901 tgacccggac cctgaaaaat tacgccgaaa aggccggcct gctggccaag ctgaagttcg
     3961 acatcaatgg cgatgatttc cgcgaagggc
//
LOCUS       DAOQXS010000282         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23301_length_3969_cov_3.422841, whole genome shotgun sequence.
ACCESSION   DAOQXS010000282 DAOQXS010000000
VERSION     DAOQXS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23301_length_3969_cov_3.422841"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="lpxK"
                     /locus_tag="PKM34_08265"
     CDS             <1..973
                     /gene="lpxK"
                     /locus_tag="PKM34_08265"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNQ83623.1"
                     /translation="ARSFHFPVIAVGNLSAGGTGKTPHIEHLIRLLKNHYKVAVLSRG
                     YRRKTKGFILCRPEHSQLEIGDEPAQYLNKFPDITLAVDEDRVRGIKRIMDERPETEV
                     ILLDDAFQHRYVKPGRSILLTDYHHLYTEDYLLPVGLLREKSKGAKRADFIIVTKTPK
                     VLSPITRRNLVKEIAPRPHQKLFYSYVSYDRPIPLKRCQNQKPAAEKYSYIIMVAGVA
                     NSYPLQEYLKTICNELIIFDFHDHHQYSRSDFEKILNEYHSIISKDKVIFTTEKDATR
                     LDTDEFSAYLDVLPVYYIPIRINFHDCDEIKFDKLILDYVRKSIGSR"
     gene            945..1772
                     /locus_tag="PKM34_08270"
     CDS             945..1772
                     /locus_tag="PKM34_08270"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNQ83624.1"
                     /translation="MFEKVSEAVDYIRTRTGLIPETGIVLGTGLGNLANEIESPVFIP
                     YDEIPHFPSSTVEGHAGRLIIGGLNGCRVVAMQGRFHFYEGYDLQDVVFPIRVMKFLG
                     INLLILSNASGGLNPGFQVGDIMIVEDHINLMNLMNENPLKGVHDERFGARFPDMSRP
                     YDAGLIKKAEILAEKMGIHYRKGIYAGVTGPTFETPAEYSYIRYLGADAVGMSTVPEA
                     IAAAQMQLPVFAVSVIADLGVKGKIVEISHKEVIDAASLAEPLMTRLIKALLGELFP"
     gene            complement(1776..2261)
                     /locus_tag="PKM34_08275"
     CDS             complement(1776..2261)
                     /locus_tag="PKM34_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83625.1"
                     /translation="MKKSVILWILLLILVRCHGQVFLLVENDKTLKNFKYYPGDVLSF
                     SLGPSQGRITDQITGMTDSTLIFEYTGEVGIDRLVTIYRSNWLMMTLSSLSMLAGAGY
                     IGLDSFNRLINREYPVIDAGTALVSAALIGGSFALMPFHDRRLKCHTRWKLKVLDQSS
                     F"
     gene            2348..3532
                     /locus_tag="PKM34_08280"
     CDS             2348..3532
                     /locus_tag="PKM34_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNQ83626.1"
                     /translation="MMAVRNIFIFRVLLLALIVLAAAGCQRDGKKSIKTTITGSFSQL
                     AGKSVSLSEFDITSATPLDTVKIDDKGNFRFKFRRKGAGFYLVKVDNRNFITLVLDQE
                     KNIRIQSSEANIRKNYSINGSPDSEIFRDFEMFTEVNRNKVDSLSKTFNNQMRSTAFR
                     SLKPELDKEYQEIFGQQVRYAQQFIENHCSSLSTLLVLNKRFGERRILTEEDNFTHFV
                     MADSCLRFHYPDNKHLKELNKKITIARQNRRIFDITEAQLAPGKTIPDISLGDVNGKQ
                     VRLYSFEGRPVILYFWASWDHSSRKANKVIRELAENSAQNPVVYAIALESYKEMWQEA
                     IRQDGLDNWINVSDLLNIKSSAKTMFNIPDTFPYFFYLDKDLYIRYKGNDFSRLETEL
                     NR"
     gene            3586..>3969
                     /locus_tag="PKM34_08285"
     CDS             3586..>3969
                     /locus_tag="PKM34_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ83627.1"
                     /translation="MINILEDNVSRSSGKIYFASDFHLGIPDHASSRQREEMLVSWLD
                     MIQSDASDIFLMGDVFDFWFEYKTVVPKGYIRLLGKLAQLTDQGLKVHLFTGNHDIWA
                     FSYLQDEAGIELHRKPEVRDMMGKTF"
BASE COUNT         1129 a          944 c          900 g          996 t
ORIGIN      
        1 ggccaggagt ttccattttc ccgtcatcgc agtcggaaac ctgagcgcag gaggaacggg
       61 aaaaacccct catatcgaac acctcatccg gttgctgaaa aaccattaca aggttgccgt
      121 cctgagccgg ggataccgca gaaaaaccaa aggatttatc ctctgccgac cggaacattc
      181 acagcttgag atcggcgatg aaccggctca atatttgaat aaatttccgg atattaccct
      241 cgccgtcgat gaagaccggg tcaggggcat caaacggatc atggatgaaa gacctgaaac
      301 agaagtcatt ctcctggatg acgcattcca gcaccgctat gtcaaacccg gaagatccat
      361 cctcctgacc gattaccatc acctgtacac agaagattat ctgcttccag tcgggctgct
      421 cagggaaaaa tccaaaggcg ccaaaagggc cgacttcatt atagttacca aaacccccaa
      481 ggtgctctcc cctatcaccc ggcgcaatct tgtgaaagag attgcccccc gtccccatca
      541 gaaactcttt tattcttatg tttcatacga caggccgata ccgttgaaaa gatgccagaa
      601 ccagaaaccg gccgccgaaa aatacagcta catcatcatg gtggccggag tggctaattc
      661 ttatcctttg caggaatacc tgaagacgat ttgtaacgaa ttgataatat ttgactttca
      721 tgatcatcac caatattccc ggagtgattt tgaaaaaata ttgaatgaat accacagcat
      781 aatctcaaaa gataaagtta tcttcaccac cgaaaaagac gcgacacgcc tggatactga
      841 tgaattctcc gcatatctcg atgtgttgcc ggtttattac atccccatca ggatcaattt
      901 ccatgattgt gatgaaataa aatttgataa acttattctg gattatgttc gaaaaagtat
      961 cggaagccgt tgattatatc agaaccagga ccgggctcat cccggaaacc ggtatcgttc
     1021 tcggtaccgg actcggaaac ctggccaatg aaattgagtc acctgttttt attccttacg
     1081 acgaaatccc ccactttccc tcttccaccg tcgaaggcca tgccggccgg ctcatcatcg
     1141 gcgggctgaa cggatgccgc gttgtggcaa tgcagggcag gtttcacttt tacgaaggtt
     1201 atgaccttca ggatgtcgtt tttcccatcc gggtgatgaa gtttctcgga attaatttac
     1261 tgatcctttc caatgccagc ggcggactaa atcccggttt tcaggtcggt gatattatga
     1321 ttgtggaaga tcatatcaac ctgatgaacc tgatgaatga gaaccccctc aaaggagttc
     1381 acgacgagcg cttcggagcg cgtttcccgg atatgagccg gccatacgat gccggattga
     1441 tcaaaaaagc cgaaatactg gccgaaaaga tgggaatcca ttaccggaaa gggatttatg
     1501 cgggagtaac cggccctacc tttgaaacac ccgcagaata cagctatatc aggtacctgg
     1561 gagccgatgc cgttggcatg tcgacagtcc ctgaagcgat cgccgccgcc cagatgcagc
     1621 tgccggtctt tgccgtatct gttattgccg acctgggggt caaaggaaaa atcgtcgaaa
     1681 tctcccacaa ggaggtcatt gatgccgcca gcctggccga acctctaatg acccggctga
     1741 ttaaagcgtt gctcggtgaa ttgttcccgt aattcttaaa atgaactttg atccagcacc
     1801 ttgagcttcc agcgggtatg gcatttcaat cgcctgtcat gaaatggcat cagggcaaag
     1861 gatccaccga tcagcgcagc ggaaaccagc gcagtaccgg catcgatcac cggatactcg
     1921 cggttgatca ggcgattgaa actgtcgaga ccaatataac cggcgccggc cagcatgctt
     1981 aagctgctca gggtcatcat cagccagttc gaccggtaaa tagtcaccag gcggtcaatt
     2041 ccgacttctc cggtatactc aaagatgaga gtagaatcag tcatcccggt gatttgatct
     2101 gtgatcctcc cttgcgatgg gccaaggctg aaactcaata cgtcgccggg atagtatttg
     2161 aagtttttaa gcgtcttatc attctcaacc agaagaaata cctggccgtg acatctgaca
     2221 agaatcagca ataatatcca gaggatgacg cttttcttca aattacccgg tgaaatgacg
     2281 atttaaacaa taaactcagg aacgaccttt atcgtatttt tgtctaaaat aataaaaatc
     2341 gggcctcatg atggcagtta gaaatatttt cattttccgg gttcttttgc tcgccctgat
     2401 tgtattagct gcagcaggat gccaaaggga tggtaaaaaa agtatcaaaa ccacaatcac
     2461 cggatccttt tcacaactcg caggaaaatc agtgtctctc agcgagtttg atattacctc
     2521 ggcgactccg ttggatactg tgaaaattga cgataaagga aatttccgct ttaagtttcg
     2581 acggaaaggc gccggatttt atctggtcaa ggtggataac cggaacttca ttactcttgt
     2641 gctcgaccag gagaaaaaca tccggattca atcatcggag gccaacatcc ggaaaaacta
     2701 ttccatcaat ggatcgcccg attccgaaat attccgcgat tttgaaatgt tcactgaagt
     2761 aaaccgtaat aaggtcgatt cgctgagcaa aacattcaat aaccagatgc gttcgaccgc
     2821 ttttcgctct ctgaaaccgg aactggataa agaataccag gaaattttcg gacaacaggt
     2881 gcgctacgca cagcagttca ttgagaatca ctgttcttcg ctgtccactt tgctggtgtt
     2941 gaacaagcgt tttggagaaa gaagaattct gacggaagaa gataatttca ctcattttgt
     3001 gatggcagat tcttgcctga gatttcatta tcctgataat aaacacctta aggaactgaa
     3061 taaaaagatt acaattgcca ggcaaaaccg ccgcattttc gatattaccg aagcacagct
     3121 ggcacccggc aaaacgatcc ctgatatctc attaggcgat gtgaatggaa aacaggtcag
     3181 gctgtattcc tttgaaggca ggcccgtcat cctctatttc tgggcatcct gggaccattc
     3241 gagccggaaa gccaataaag tcattaggga actggccgaa aattcggctc aaaatccggt
     3301 ggtatatgcc attgcgctgg aaagctacaa agagatgtgg caggaagcta tccggcagga
     3361 cgggctcgat aactggatca atgtttctga tctcctgaac attaaatcat cggcaaaaac
     3421 aatgttcaac atacccgata cgtttcctta tttcttttac ctggataaag atctgtatat
     3481 ccgttacaaa ggaaatgatt tcagcaggct ggaaactgaa ttgaatcgtt aagttgccac
     3541 gccgcaattg gattataagg ggaagtttag taatattgcg ttaaaatgat caatattttg
     3601 gaagataacg tttcccggtc atccggaaaa atctacttcg cttccgattt tcacctgggc
     3661 atccccgacc atgcatcgag ccgacaaagg gaagagatgc tggtttcctg gctcgatatg
     3721 atccaatccg atgcttcgga tatttttctc atgggtgatg tttttgattt ctggtttgaa
     3781 tacaagaccg ttgtcccaaa aggatatata aggttattgg ggaaattggc ccaactgacc
     3841 gaccagggtc tgaaagtgca cctttttacc ggtaaccacg acatctgggc tttcagctat
     3901 ttgcaggatg aagcaggaat agaacttcac cgcaagccgg aagtcaggga catgatgggc
     3961 aaaaccttt
//
LOCUS       DAOQXS010000283         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23412_length_3956_cov_3.743912, whole genome shotgun sequence.
ACCESSION   DAOQXS010000283 DAOQXS010000000
VERSION     DAOQXS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23412_length_3956_cov_3.743912"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..735
                     /locus_tag="PKM34_08290"
     CDS             250..735
                     /locus_tag="PKM34_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNQ83628.1"
                     /translation="MIQRIQSLFLILAALASILLFFFPLAGIYASHATYKFYIYELQN
                     QVPLEPGEQPIFTFMTTLPLLVLNIGVIILTVITIFLYKNRITQMKLVRFAVLLEIVM
                     IALILFIYASIIEKNLFVSPDYLSEAGIYFPLVALVFLILANRSILKDEKLVRSVERL
                     R"
     gene            complement(736..1551)
                     /locus_tag="PKM34_08295"
     CDS             complement(736..1551)
                     /locus_tag="PKM34_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNQ83629.1"
                     /translation="MKTPALFLAYLMLISFSGAINIYGQSSSPVKPFGEPEFIQDFLC
                     SEVIYPETELSEGIEGKVIISFTVEPDGQVSQFGLKENISPGLDAEAVRLFGMLLWEP
                     ATEFGNPVRSVGEFPVDFNIKKYKKHCKARGYERTELPFQPVDSSNIVYETGQTDRKP
                     YPLFSEPGMSFNRFISQNISYPETAYRQGISGTVTLKFVIETSGRASNIKIINPVGGG
                     CSQEAIRLLKLLRWMPGIKGQGAVRTFMKIDIGFKLPENSEMNMFENSQMNPN"
     gene            1641..2963
                     /gene="rmuC"
                     /locus_tag="PKM34_08300"
     CDS             1641..2963
                     /gene="rmuC"
                     /locus_tag="PKM34_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNQ83630.1"
                     /translation="MIILYSGIALIIGVLITWLIGRTVSGKKVAFLKEQLAESDKENG
                     ILNERLMQARSEILKLEKSLEDERSAKSELVAGHARQAGEIDNMREKLTTQKSELEEL
                     QKKFATEFENIANRLLRENSREFTHLNQKNIGDILVPLREKIEKFEKKVDDTYQKGLV
                     EQTQLKDAIENLHKLNVRISEEANNLTRALKSDSKKMGNWGELILDRILEQSGLEKGK
                     EYYTQYTDRSEEGTVLRPDVVIALPDKKHIIIDSKVSLVAYDRFINGSSDEEKEKWQK
                     AHLESVREHIRGLSEKNYTGALTLDTPDFVLLFMPLESAFSLAVQHDPELFGYAWQRR
                     IVIVSPTTLLATLKTVESIWKHEKQTQNAMEIARQGKNLYDKFVNFIEDMEKLGNQIN
                     SVQNTYQDAHKKLTSGKGNLVRQAQNLISLGVKTEKSLSDKILPEDEE"
     gene            2953..>3956
                     /locus_tag="PKM34_08305"
     CDS             2953..>3956
                     /locus_tag="PKM34_08305"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83631.1"
                     /translation="MKSNLVHRLLWLLFIGMGVLTGCGTTGKVSLQNLVYLYHPDKQF
                     ATLNNCVYHTSDSTSTLFVEVLFNDLVYLKDPFTGLYACNYRLSYNLKEGYEKKEVLH
                     SSAVISGDSLNYGKSAKKVHSFELKTFYPEKYLLEITLYDLNRSGGTTVYREVDKSSR
                     QTRQNYLLLDINNELIWKDQRVEGEPFRIVSNRNDLNFLIVNRYVRDFPVARPPYTEE
                     NEPSFDYKPDSLYLQPVFNGQTDWIRLAGPGFYHFRQDTISRDGVTIFLYPEGFPEVT
                     TAGQLRYPLRYITTRKEYDTLTESENVKAAVDAFWLKTARTPDRAQLLFKKYYSNVED
                     A"
BASE COUNT         1069 a          924 c          960 g         1003 t
ORIGIN      
        1 ttctctttgc gtgtaagctc caggaacagg atcagtcctc ccgccacaaa acccatcgcc
       61 acaaagaacc cataggtctg gaagggcagg tggagattgg ttcccaggag atcgtttatc
      121 aggtcgctca gttttggata cataaataga ggattaattt ctttcgtgcc ggcctgatgg
      181 caaaattaac aagttatctt tgcattcggg tttttactga attcaatttt tatctgtaag
      241 catcgcatta tgattcaacg tattcaatca ttgttcctga tcctggcggc cttagcctcc
      301 attttgctgt tctttttccc gctggccggc atatatgcca gtcatgccac gtataaattc
      361 tatatctatg aactgcaaaa ccaggttccg ctggaaccgg gagaacagcc gatatttacc
      421 ttcatgacca cgttgccttt gctggtgctg aatatcgggg ttatcatcct tacggttatc
      481 actatctttc tttacaaaaa caggataaca cagatgaaac tggtccggtt cgccgtgttg
      541 cttgagatag tgatgatcgc gctgatcctg tttatctatg ccagcattat cgagaaaaat
      601 ctgtttgttt cacccgatta cctgagcgaa gcgggaatct attttccgct ggtggcgctg
      661 gttttcctga tcctggccaa ccggtcgatt ttaaaggatg agaagctggt ccgttcggtc
      721 gagcgcctcc gctgatcagt tcgggttcat ctgggagttc tcgaacatat tcatttcaga
      781 attctccggc agtttaaagc cgatatctat cttcatgaaa gtcctgaccg ctccctggcc
      841 tttaataccg ggcatccagc gtagcaattt taagagtcgt attgcctcct gcgagcatcc
      901 tccacctacg ggattgataa tctttatgtt agaggccctg ccgctggttt ctataacaaa
      961 ctttagggta acggtgcctg agatcccttg cctgtaagcc gtttcagggt aacttatatt
     1021 ttgtgagatg aaccggttaa agctcatccc cggctcggaa aacaacggat atggctttcg
     1081 gtcggtctgg ccggtttcat aaacgatatt cgacgaatcg accggctgga aggggagttc
     1141 tgtacgttcg tatcccctgg ctttacaatg tttcttgtat ttttttatat tgaagtcaac
     1201 cggaaattca ccgactgacc tgacaggatt cccaaattcg gtggccggct cccagagcag
     1261 cataccgaac aacctgaccg cttcggcatc gagcccgggc gaaatattct cttttagccc
     1321 gaactgactg acctgtccat ccggctctac ggtgaacgat atgattactt ttccctctat
     1381 tccttccgac agttccgttt ccgggtaaat gacttccgaa caaagaaaat cctggataaa
     1441 ttcgggttca ccaaaaggct ttacaggact ggaactttgt ccgtaaatat tgatcgcccc
     1501 ggaaaaggat ataagcatca aatacgccag aaataatgcc ggtgttttca ttaccaaagg
     1561 tttgtggtaa atgtaaggaa atttgcaccg taatgcaaga atgactttcc gaaatggtta
     1621 tttttatcga aaaaatggtc atgatcattt tatattcggg aattgcatta ataatcgggg
     1681 tgttaattac ctggctgatc gggagaactg tttcgggcaa aaaggtcgct tttctgaaag
     1741 agcagctcgc ggaatcggat aaagaaaacg ggattttgaa cgaacggttg atgcaggcac
     1801 gcagtgagat cctgaaactg gagaaaagcc ttgaagatga gcgaagtgca aaatctgagc
     1861 tggttgccgg ccatgcccgc caggccggtg agattgacaa catgagagaa aagctcacca
     1921 cccagaagag cgaattggaa gagttgcaga agaagtttgc aaccgagttc gagaatattg
     1981 ccaaccggct tctccgtgag aattcccggg agtttaccca tctgaatcaa aagaatattg
     2041 gagatatcct ggtaccactg cgggagaaaa ttgagaaatt tgagaaaaag gtcgacgata
     2101 cttaccagaa aggactggta gaacaaaccc agcttaaaga cgccattgag aacctccaca
     2161 agctcaatgt caggatcagc gaagaagcca acaacctgac gcgggccctg aagtccgatt
     2221 ccaagaagat gggcaactgg ggcgagctga tcctcgaccg gatactggag cagtcagggc
     2281 tggaaaaagg caaggagtat tatacccagt acaccgaccg gtcggaagag ggtaccgtat
     2341 tacggcccga tgtcgtaatc gcactgcccg ataagaaaca tatcattatc gactcaaaag
     2401 tatcactggt cgcttatgac cggtttatca acggaagcag cgacgaggag aaggaaaaat
     2461 ggcagaaggc ccacctggaa tcggtcaggg aacatatccg gggattgagc gaaaagaatt
     2521 acacaggtgc tttgactttg gatacacccg acttcgtact gctgttcatg ccgctcgaat
     2581 cggctttcag cctggcagtc cagcacgacc cggaactgtt cggttatgcc tggcaacgga
     2641 ggatcgtcat cgttagcccg acaactttgc tggcgaccct gaaaacagtc gaatcgatct
     2701 ggaagcacga aaagcagacg cagaatgcga tggaaatagc gcgccagggg aaaaacctgt
     2761 acgataaatt cgtgaacttt atcgaagata tggaaaaact gggaaaccag atcaattccg
     2821 ttcagaatac ataccaggat gcgcacaaaa agctgaccag cgggaaggga aacctggtgc
     2881 gccaggctca gaacctgatc agcctgggag tcaaaactga gaagtcgctg tcggataaaa
     2941 tacttcccga ggatgaagag taatcttgtg caccggttac tttggctgct gttcatcgga
     3001 atgggggttc tgaccggctg cggcacgacc ggaaaagtca gccttcaaaa cctggtgtat
     3061 ctctaccatc ctgataaaca atttgccaca ctcaataatt gcgtttacca cacgtccgat
     3121 tccacttcaa ccctttttgt tgaggtgttg ttcaatgatc tggtttatct gaaagaccct
     3181 tttaccggtc tttatgcctg caattacagg ctcagctata atctgaagga agggtatgaa
     3241 aagaaggagg ttctgcatag ctcggctgtt atatccggtg attcgctgaa ctacggaaag
     3301 agtgcaaaaa aggtccattc gtttgaattg aaaacttttt atcccgaaaa gtacctgctt
     3361 gagattacct tatacgattt aaaccggagc gggggcacaa ccgtttaccg ggaggtcgat
     3421 aaaagttccc ggcagacgcg gcagaattat cttcttttgg atatcaataa tgagttgata
     3481 tggaaggatc aacgggtgga aggagagccg ttccggatcg tatcgaaccg gaatgacctc
     3541 aatttcctca ttgtcaaccg ttatgtccgg gactttcctg tagccaggcc tccttacacc
     3601 gaagaaaatg aaccatcctt tgattacaag cccgacagcc tttaccttca gccggtgttc
     3661 aacgggcaga ccgactggat caggcttgcc ggcccgggat tttaccactt ccggcaggat
     3721 accatttccc gtgacggcgt aaccattttc ctttatcctg aaggttttcc ggaggttacg
     3781 actgccgggc aactgcgata tccgctgcga tacattacta cgcgcaagga atacgatacc
     3841 cttaccgaat cggagaatgt caaagccgcc gtcgatgctt tctggctgaa aacggcccgc
     3901 actcccgacc gggcgcaatt gttgttcaaa aaatattatt cgaatgtcga agatgc
//
LOCUS       DAOQXS010000284         3955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23422_length_3955_cov_3.422308, whole genome shotgun sequence.
ACCESSION   DAOQXS010000284 DAOQXS010000000
VERSION     DAOQXS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23422_length_3955_cov_3.422308"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKM34_08310"
     CDS             <1..854
                     /locus_tag="PKM34_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ83632.1"
                     /translation="GPSRVVLETIYTRFGVEYTMVDTTDIEKVKTAFRPNTKLLFTET
                     PANPTMGISDLPALAELAHSHNVPLVVDNTFCSPYLQNPLDLGADIVLHSMTKFINGH
                     ADIVAGMIVAKKEEDYKKLRGIMTNLGCNMDPHQAFMTRRGLKTLAIRIDRAQENAMV
                     IAGYLEKHPKVAWVKYPGLKSHPQYELGKRQMKGPGAMISFELKGGLTAGKVLMDNVK
                     VMLLAVSLGGIETLIQHPASMTHSKVSAENRHHAGITDGLVRLSVGIEKVDDLLADLD
                     QALGKII"
     gene            1054..3378
                     /locus_tag="PKM34_08315"
     CDS             1054..3378
                     /locus_tag="PKM34_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNQ83633.1"
                     /translation="MTEKDLTADNIQQELPENTEITSEPSATPEAETTEIPAEEILTE
                     QAEIQEIITETIPTEEIPVQDIVPDDSPLAEALTDDVPVEISPAEVIPEVIAPVEEIP
                     VEEVPTENASDPEPAVEDVPVEEVAAEDVPEEKPVAEDAEPEPVAAEEQTATELHPEV
                     TADAALLQEIAEMDHSGEQETEEEEEDILEEHEEIVERYDTYDREKLVEILEETVKEE
                     NIVRIKTRVAMIKVAFLRLSKEFRQQLLDNHLTAGGTRENFTPEPDPLVEKFNAVFET
                     YKKNKIKFNEHQEVVKLRNLDAKKAILEELKELINSEETLKKTYDQFKELQTKWKEIG
                     LVPSTEINNLWQNYHFLVEKFFDKVKISKELRDLDLRKNLEHKIELCEKAEELLIEPS
                     IIKSFQQLQKYHEEWKEIGPVPQDKRDEIWERFKTATDKINERRREHYNILAKDQDEN
                     LAAKNALVEKAEQLASIEVDELKGWQEKTREVNELFRQWKTIGPAPKKENDEVWEKFK
                     AYLNAFFANKKEYFSRIKQEQLNNFNLKLDIVKQAEALKDSTDWRKTTQDLINLQKEW
                     KNIGPVPRKHSDKIWKRFRAACDEFFNNKSKHFSTVVESEKDNLDKKLALIKQVEEWE
                     AGKDKSANLEALKGFQREFMEIGHVPIKEKNKVHDAFRQAINKHFDNLKLSSYEGRPQ
                     SEYREYRSRQDSYDGDRYQRRGGSTGGGSSEWSALSTKVSKLREEIMLWENNIGFLAH
                     SKKADLLRAEFEKKIQDAKAELASLEAKLKSMQE"
     gene            3385..>3649
                     /locus_tag="PKM34_08320"
     CDS             3385..>3649
                     /locus_tag="PKM34_08320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ83634.1"
                     /translation="MSGNSIGISFKLTTFGESHGPATGGVIDGCPAGLPIDPEFIQQE
                     LDRRKPFYEGATGRKEADAVEFLSGIGNGISLGTPIAFIIRNED"
     assembly_gap    3650..3659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3660..>3955
                     /locus_tag="PKM34_08325"
     CDS             <3660..>3955
                     /locus_tag="PKM34_08325"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ83635.1"
                     /translation="TSDYQEIKDVFRPSHADYSYFMKYGRMATGGGRASGRETAARVA
                     GGAVAKLLLGKAGISVKGKVQSIGDGTAEGISKAIEQGDTLGGTVTCRIAGVPAG"
BASE COUNT         1181 a          972 c         1039 g          753 t
ORIGIN      
        1 atggcccgtc gcgcgtggtt ctcgaaacga tctatacccg gttcggcgtg gaatatacaa
       61 tggtcgacac caccgatata gaaaaagtga aaaccgcttt ccgtcccaac acaaaattgc
      121 ttttcaccga gactcccgct aatccgacca tggggatcag cgacctgccg gcgctggccg
      181 aactggccca cagccacaat gtaccgctgg tggtcgacaa tactttctgc agcccgtatc
      241 tccagaaccc gctggatctg ggtgccgaca tcgtcctcca ttcaatgacc aaattcatca
      301 acgggcatgc cgatatcgtg gccggaatga tcgttgccaa aaaagaggaa gattataaga
      361 aactccgggg tattatgacc aacctgggct gcaatatgga cccccaccag gcttttatga
      421 cccggcgcgg gctcaaaacg ctggcgatcc gtatcgaccg ggcgcaggaa aatgccatgg
      481 tcatcgccgg atatcttgaa aaacacccga aagtggcctg ggtaaaatat ccgggactga
      541 agtcgcatcc gcagtatgaa cttggaaaaa ggcaaatgaa aggccccggc gctatgatca
      601 gcttcgagct gaaaggcggc ctcactgcag gcaaagtgct gatggacaat gtaaaggtga
      661 tgctgctggc cgtatcgctc ggtggaattg aaacccttat ccagcacccg gcctccatga
      721 cccattccaa agtcagcgcc gaaaacaggc accacgccgg gattaccgac ggactggtgc
      781 ggctctcggt aggtatcgaa aaagtggatg acctgctggc cgacctggat caggcgcttg
      841 gcaaaataat ctgaaataat gaccggaaca atccgctaat ttttcatctt atttcattca
      901 ttttaaataa atgctatttt tgcagacctt aaaattttga gctatatcat ccgtaaagga
      961 taacatctat ttataattaa cacaacacag aaaccttatc tcactcttta gcctgaaaac
     1021 cagagaggtg agaacacaaa cagtaatttt gccatgacgg aaaaagatct gaccgctgat
     1081 aacattcagc aggaactccc cgaaaacaca gaaatcactt ccgaaccctc cgcaactccg
     1141 gaagcggaaa ccacggaaat cccagccgaa gaaattctta ccgaacaggc tgaaattcag
     1201 gaaatcatca cagaaacaat cccaacggaa gaaatcccgg ttcaggacat tgtgcccgat
     1261 gactcaccgc tggcggaggc tttgacagac gatgttccgg ttgaaatctc gccagcggag
     1321 gtaattcccg aagtaattgc tccggttgaa gaaattccgg ttgaagaagt tccgacggag
     1381 aatgcttctg atccagaacc cgcagtcgaa gatgtcccgg tagaagaggt tgctgctgaa
     1441 gatgttccgg aagaaaagcc tgttgcagaa gatgcggagc ccgaacctgt tgctgcagaa
     1501 gagcagactg caaccgaact gcaccctgaa gtaacagccg atgcagctct gttgcaggag
     1561 attgccgaaa tggatcattc cggtgaacag gagacagaag aggaagaaga agatatcctg
     1621 gaagagcatg aggagatcgt agagagatac gatacctacg accgggaaaa actggtggag
     1681 atccttgaag agaccgtcaa agaagaaaat attgtcagga tcaagacccg cgtggccatg
     1741 atcaaagtgg cgttcctccg cctgtcgaag gaatttcgcc agcagttgct cgacaaccat
     1801 ctgactgcag gagggacccg ggagaatttc accccggaac ccgatccgct ggttgaaaag
     1861 ttcaatgccg tttttgagac atacaagaag aataaaataa aattcaatga gcaccaggaa
     1921 gtcgttaaac tacgcaacct ggatgcaaaa aaagccatcc tggaggagtt gaaggagctg
     1981 ataaattcgg aggaaacact caaaaagacc tacgaccagt tcaaggaact ccagacgaaa
     2041 tggaaagaga tcggcctggt gccttccacc gaaatcaata acctctggca gaattatcac
     2101 ttcctggtcg agaagttttt cgacaaagta aagatcagca aagagcttcg cgatctcgac
     2161 ctgaggaaga acctggaaca taagatcgaa ctgtgtgaaa aagcggaaga gcttctcatc
     2221 gagccatcca tcattaaatc gttccagcaa ctgcagaagt atcacgaaga gtggaaagag
     2281 atcggaccgg tgccgcagga caagcgcgac gagatctggg agcggttcaa gacagctacc
     2341 gacaagatca acgaacggcg cagagaacat tacaacatcc tggccaaaga ccaggatgag
     2401 aacctggcgg ccaagaatgc cctggtggaa aaggcagaac aactggcttc aattgaggtc
     2461 gatgaactga aaggctggca ggagaaaacc cgcgaagtga acgaactctt ccgccagtgg
     2521 aagaccattg gccctgctcc caaaaaagag aacgacgagg tatgggagaa attcaaagct
     2581 tacctcaacg ctttcttcgc caataaaaag gagtacttct cccggatcaa acaggagcag
     2641 ctcaataatt tcaacctgaa gctcgacatc gtcaaacagg ccgaagcgct gaaagacagc
     2701 accgattggc gcaagactac ccaggacctg atcaatttgc agaaagagtg gaagaatatc
     2761 ggcccggtgc ccaggaaaca ttccgacaag atatggaaac ggttccgtgc cgcctgcgac
     2821 gaattcttca ataataaatc caaacatttc tccacagttg tcgaatcgga aaaagataac
     2881 cttgacaaaa agctggcctt gatcaagcaa gtggaagagt gggaagccgg gaaggacaaa
     2941 tcggccaacc tggaagccct gaaaggattt cagcgggagt tcatggagat cggacatgtg
     3001 ccgatcaagg agaagaacaa ggtacacgat gcttttcgcc aggctatcaa caagcatttc
     3061 gacaatctca agttaagctc ctacgaaggc aggccccagt cggaataccg ggaataccgt
     3121 tccaggcagg attcttacga cggcgaccgt taccagagac ggggcggcag taccggcggc
     3181 ggcagcagcg aatggagtgc cctgagcaca aaagtgagca agttgagaga ggagatcatg
     3241 ctgtgggaaa acaatatcgg tttcctggcc cattccaaaa aagccgacct gcttcgggcc
     3301 gagttcgaga agaagatcca ggatgcgaaa gcggagctgg cctctctcga agcaaagctg
     3361 aaatcgatgc aggagtgatc ttgcatgtcc ggtaattcca tcggtatatc gttcaaactg
     3421 actacattcg gcgaatcgca cggcccggct accggtggcg tgatcgatgg ctgcccggcc
     3481 ggattgccta ttgaccccga atttatccaa caggagctgg accggcggaa accgttctac
     3541 gaaggggcta ccggcagaaa ggaggccgat gccgtagaat tcctttccgg catcgggaac
     3601 ggtatttcat tgggaactcc catcgccttc atcatacgta atgaagatgn nnnnnnnnna
     3661 catcagatta ccaggagatt aaagatgttt ttcgcccctc gcacgccgat tacagctatt
     3721 ttatgaagta cggcaggatg gcaacaggag ggggacgtgc atcgggacgt gaaacggccg
     3781 cacgggttgc cggaggcgcc gtggctaagc tactgctggg aaaggccggt atatccgtta
     3841 aaggcaaggt gcaatcgatc ggtgatggca cagccgaagg catatccaaa gcaatagagc
     3901 agggcgatac gctcgggggt acggtgacct gccgcattgc cggggtacct gcggg
//
LOCUS       DAOQXS010000285         3948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23474_length_3948_cov_3.627793, whole genome shotgun sequence.
ACCESSION   DAOQXS010000285 DAOQXS010000000
VERSION     DAOQXS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23474_length_3948_cov_3.627793"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKM34_08330"
     CDS             <1..339
                     /locus_tag="PKM34_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83636.1"
                     /translation="DRIYDVLLALNRFPGHDTATTRVLFVNFGEKEELFCLGLASRLR
                     EEGISTEIYPDPDKLKKQMKYADQKQIPLVVLAGENEIGSGILTVKNMKTGEQMNLTP
                     GQLVNFCKET"
     gene            complement(336..674)
                     /locus_tag="PKM34_08335"
     CDS             complement(336..674)
                     /locus_tag="PKM34_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ83637.1"
                     /translation="MIIRIDKAEKLKEIVGTAENLLVYFLNDDCPPCLSLRPKVEQLV
                     SERFPLMDLVYVNAREYPALLAEYQVFSFPVLIFFFEGKEYLRYSKYVSMAELGESIG
                     RIYDLYHLQD"
     gene            923..2446
                     /locus_tag="PKM34_08340"
     CDS             923..2446
                     /locus_tag="PKM34_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNQ83638.1"
                     /translation="MTIVITGSNLKIKDVVNVARHNEKVELHPDAVKRIETCRAMLEK
                     KIVAREIMYGVNTGIGEFSEIVLTDEQVQQFQRYLIYNHAAGIGDPMPIEWVRGAMVG
                     RINVHAHGNSGNRPVITQTLVEMLNKGVTPYVCQKGSVGACGDLAPMSQIALLMMGEG
                     KAYYQGELLDGKEALDRAGIPVPGLQARDGLATINGSNVLTAMSALFLYDANNFLKQA
                     EIAASMSLEALKANMKPYNIKLHSLRGFPGAVRSAAAINKLVSGGDLKEGKIKCKVQD
                     AYSMRSTPQVIGAAHDAIAWAVKQVETELNGVGDNPIFIPEDNLTLTGANFQGSPVAL
                     PLDMAGAAITMVCVLSERRMNRLNNPALSVGLPAFLTKGAGMFSGLMLSQYTADMQIV
                     EQRILSAPASIQSIPAAADQEDFVSMGMNVALKNFQIIDNAYGILGIELMAAAQALDF
                     REFSFGNGVQKAKEVIRRHVAFLDVDRPLYDDHNTMKALVKSCEILTEVEKEIGSLE"
     gene            2484..2921
                     /locus_tag="PKM34_08345"
     CDS             2484..2921
                     /locus_tag="PKM34_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83639.1"
                     /translation="MTDNGSENIVTSFQKEIRKGSFICLVVQCMALTYFLIIALLFLA
                     GLLFSGTVNEAVGTYYGLEEEFASKYFLFIITGTVLFLTIVAGIIIFMMKYRAGFYIF
                     IAAILVTIILDLTFLKFDWVRYLIQSGFIFLLGIAHFSRKCYR"
     gene            3189..>3948
                     /locus_tag="PKM34_08350"
     CDS             3189..>3948
                     /locus_tag="PKM34_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ83640.1"
                     /translation="MRKFTLFAVFLLFVGMQAALAQMVVRGTITDAKDGTPIPGVSVV
                     VKGTLTGTVSDAMGKYELDVPAGYNELIFSFIGMRTKEMKIDNKSVVDIAMDEDVVGL
                     DEVVVTALGISREKKSLGYSTQEATGVEISKTANPNFQTALSGKFAGVEVRQSSGMPG
                     APSQIFIRGARAFSGDNTPMYVVDGMPIVSTPDYEQASGGVTGSYYSNRALDIDPNNI
                     ESINVLKGQAAAALYGMRASNGVIIITTKKGKVGQ"
BASE COUNT         1047 a          901 c          989 g         1011 t
ORIGIN      
        1 gaccgcattt acgatgtcct gctggcgctg aaccggtttc ccggccatga taccgcaaca
       61 acccgggttt tgtttgtgaa cttcggagaa aaggaagaac tgttttgcct tggcctggcc
      121 tcacggctca gagaagaagg gatcagcact gaaatttacc ctgatccgga caaattgaag
      181 aagcaaatga aatatgccga ccagaagcag atcccgctgg tggtcctggc cggggaaaat
      241 gagatcggca gcgggatttt gaccgtcaaa aacatgaaaa ccggcgagca gatgaacctg
      301 acgcccggtc agttggtcaa cttttgcaaa gaaacctagt cctgcaagtg gtatagatcg
      361 tatatccggc cgatcgattc acccagttcc gccattgaaa catatttgct gtaacgcaaa
      421 tactctttcc cttcaaaaaa gaagatcaat acagggaagc tgaacacctg gtattcagcg
      481 agtaaggcgg gatattccct tgcgttgaca taaaccaggt ccattaaggg gaagcgttcg
      541 ctgaccagtt gttcgacttt cggtcggagg ctaaggcagg ggggacagtc atcattcagg
      601 aaatagacga gcagattttc ggctgtcccg acgatctcct tcagcttttc tgctttatcg
      661 atccggatta tcatagggcg aattttcgcg gcaaaaatat atctttaata atatcaggga
      721 gattaatttt ttacttttgc cataaatgat ctatcaaatg tttagctgga ttcttaaatt
      781 tataataaag atcaaccgca ccgcactttg ctaaatttta aattagaaat tttgaatttt
      841 taattcggaa aactttcagg tttttcaatt ctaaattctt cctcattgca aattcattat
      901 aaataattta aaatttaaaa ttatgactat tgttataact ggttccaatt tgaagataaa
      961 agacgtggtc aatgtggccc gtcataacga aaaagtagaa ttacacccgg atgcggtaaa
     1021 aaggattgag acatgccgtg ccatgctgga gaagaagatc gttgcacgcg agatcatgta
     1081 tggcgtcaat acgggcatcg gtgaattttc ggagattgtg ctgacggatg agcaggttca
     1141 gcagtttcag cggtatctca tttataatca tgcagccggt atcggcgatc cgatgcccat
     1201 cgaatgggtg cgcggcgcca tggtaggccg gatcaacgta catgcccacg gcaattcggg
     1261 caaccggccg gtgatcaccc agacgctggt ggagatgctg aacaaagggg taacgcccta
     1321 tgtatgccag aaagggtcgg ttggcgcctg cggcgacctg gctcccatgt cgcagatcgc
     1381 cctgcttatg atgggcgaag gcaaagccta ttaccagggc gaactgctag atggtaagga
     1441 agcccttgac cgtgccggta taccggttcc gggattgcag gcacgggatg gcctggctac
     1501 gatcaacgga tcgaacgtgc tgacagccat gagcgccctt ttcctgtacg acgccaacaa
     1561 tttccttaaa caggctgaga tcgccgcttc catgtcgctc gaagcgttga aagccaatat
     1621 gaagccatac aatatcaagc tgcactccct gaggggattt cccggggctg taagaagcgc
     1681 ggcggctatc aacaagctgg tttccggcgg cgacctgaaa gaggggaaga tcaaatgcaa
     1741 agtccaggat gcctactcta tgcgatcgac gccgcaggtg atcggcgcag cgcacgatgc
     1801 catcgcctgg gcggttaaac aggtggaaac cgaactcaac ggcgttggcg acaacccgat
     1861 cttcatcccg gaagacaacc tgacactcac gggggccaat ttccagggga gcccggtggc
     1921 cttaccgctt gatatggccg gagcggctat tacaatggtg tgcgttttat ccgaaagaag
     1981 gatgaacagg ctgaataacc cggccctgag cgtagggctg cctgcatttc tcaccaaagg
     2041 cgccggcatg ttctccgggc tgatgctgag ccagtacact gccgatatgc agatcgtcga
     2101 gcagaggatc ctctcggctc cggcatccat ccaatccatt cccgccgcag ccgaccagga
     2161 agactttgtt tcgatgggaa tgaacgttgc gctcaagaat ttccagatca tcgacaatgc
     2221 atacggaatc ctgggcattg agctgatggc agccgcgcag gcgctcgatt ttcgcgaatt
     2281 cagctttgga aacggggttc agaaagccaa ggaggtcatc cgcaggcatg tggcattttt
     2341 ggatgtcgac cggccattgt acgacgacca caacaccatg aaggccctgg taaaatcgtg
     2401 tgaaatcctg acagaagtcg aaaaagagat cggcagcctg gaataatcat taacttagct
     2461 ggttcatttt cattcagatc atcatgaccg ataatggatc agaaaatatt gtcacatctt
     2521 ttcaaaagga gatcaggaaa gggtcattca tatgcctggt ggtacagtgc atggccctta
     2581 cctatttcct gatcattgca ctgctgtttc ttgcagggct tctcttctca ggtactgtca
     2641 atgaagcggt aggcacttat tacggcctgg aggaagaatt tgcgtcgaag tattttttgt
     2701 tcattatcac cggtactgta ttatttctga cgattgtagc cggaataatc atttttatga
     2761 tgaagtacag agccggattt tatattttta ttgccgctat cctggtcaca ataatccttg
     2821 atctcacttt tctgaaattt gactgggtcc ggtacctcat tcaatccgga tttattttcc
     2881 tgctgggtat tgcccatttt tccaggaaat gttatcgcta gtcattctaa ttcaatctgt
     2941 tcgaaataat aggttctgta aggggctgat ccggattttc gacgataaag taagactccg
     3001 tataaattaa tagtcagttt ttttcttcgc aaaaaggcta catatcacaa tttcaatacg
     3061 taaaataaaa ttagtgttaa tttttggtca aattttttca tctggtgcgc ttgtatttct
     3121 atatttatta ttgtaatatt gcagtagaag aagcgagttc aattttctat taaccaaaaa
     3181 ttacttttat gagaaaattt acactttttg ccgtatttct acttttcgta gggatgcaag
     3241 ccgctttggc ccaaatggtg gtcagaggga cgataactga cgcaaaagac gggactccaa
     3301 tcccgggggt ttcggttgtc gttaaaggca cgctgacggg aacagtctcc gatgccatgg
     3361 gaaaatatga attggatgtt cctgcaggct acaatgagct catcttttcg tttatcggga
     3421 tgcgcaccaa agagatgaag attgacaaca aatcggttgt cgacattgcc atggatgaag
     3481 acgttgtcgg tcttgatgaa gtggtcgtca cagctttggg tatctccagg gaaaagaaat
     3541 cactgggtta ttcgacacag gaagcgaccg gggtggagat atcaaagact gccaacccga
     3601 acttccagac ggcactttcc gggaaatttg ccggcgtcga agtccgccag tcatccggta
     3661 tgccgggagc accgtcacag atctttatcc gtggtgcccg tgcatttagc ggtgacaaca
     3721 ctcctatgta tgtggtcgat ggaatgccaa tcgtcagtac gcccgactac gagcaggcga
     3781 gcggtggcgt caccggctca tactacagca acagggcatt ggatatcgac ccgaacaaca
     3841 tcgagtcgat caacgttctg aaaggccagg ctgccgctgc cctttacggg atgcgtgcat
     3901 ccaacggcgt tatcatcatt acaaccaaaa aaggcaaagt cggccagg
//
LOCUS       DAOQXS010000286         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23518_length_3944_cov_3.817691, whole genome shotgun sequence.
ACCESSION   DAOQXS010000286 DAOQXS010000000
VERSION     DAOQXS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23518_length_3944_cov_3.817691"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKM34_08355"
     CDS             <1..720
                     /locus_tag="PKM34_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNQ83641.1"
                     /translation="EGKFEMADKGTLFLDEVADMSLTLQAKLLRVLQEGTFERLGSNK
                     VMKVDVRVISATNKNLENAIAGKNFREDLYYRLKVISITLPPLRVHPEDIPLLTNYFL
                     SRHCKEMKKPQVTVPPETMEALLRHSWPGNVRELENMLKRALLLSKNNVITPDLVHSE
                     IQSNVPVTKDQPHPAYPSLIPDNMEDFEGVLFKHVIEKVEKEIINKALAHCKGNQVKT
                     AKFLGISRAMLIERMKNYGLT"
     gene            964..1536
                     /locus_tag="PKM34_08360"
     CDS             964..1536
                     /locus_tag="PKM34_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ83642.1"
                     /translation="MAELFNFRYRVPSARANWWNYGDNGAYFITICTKYRVHYFGRII
                     NHQMDLSIIGQHAWDCWLQIPEHFPFVELGAFVVMPNHIHGIIIINKKNIEIPDKRDK
                     KGMVTDHHENAAKNQFGAQSQNIPSIVRGLKIGVTKFCKHENLPFGWQPRYHDHIIRN
                     AKEYERISNYILNNPAKWKEDIHNAGNRLL"
     gene            1734..2339
                     /locus_tag="PKM34_08365"
     CDS             1734..2339
                     /locus_tag="PKM34_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83643.1"
                     /translation="MKRNVLLTIAALAGMTLLFSSCAKLPEAEMAAAKAAVEAAKAAE
                     ADRYIPAEYRAVNDSLNVAMTEVENQKSKFVLFRSYKKVTVKLNNVVTLANTVKENAG
                     IRKEEVKNQAQQTLAESTTLVEEVKALILKAPKGKEGKEALEAIQSDLALVEASLAEV
                     STLINNGDYLTALDKVKAANEKAGSLKAELEEAIAKKTMKK"
     gene            2490..3614
                     /locus_tag="PKM34_08370"
     CDS             2490..3614
                     /locus_tag="PKM34_08370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNQ83644.1"
                     /translation="MKRKRKIKLSRVLIALGAFCALIALVFGVLLLTSSKPPVYAITI
                     CQTEISRAREVEADRYAAEMLIDAESTCQLAMNEWKYQNARVFFARDYDQMLNLAMAS
                     ANKARHASEQALHIKDSLQSGLSDKLKSVSHKIDHFDEYYAHLPLNGTTRQNFTTAKL
                     RYMESKEAYERGDYKQVGVNLDVASQLITRSVAEAHNYLSDYFTSLSKWKRWADETIA
                     WSKNNNAYAILVDKFAHKCYLYHDGKVKKEFTAELGPHWIGTKQYRGDKATPEGKYHV
                     TKKKANPHTKYYKALLINYPNDEDKARYEANVRKGNIPRRGIGNLIEIHGDGGKGINW
                     TDGCVALTNSDMDKLYGLVGVGTPVTIVGSLRSLKEINGF"
     gene            3643..>3944
                     /locus_tag="PKM34_08375"
     CDS             3643..>3944
                     /locus_tag="PKM34_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83645.1"
                     /translation="MDDRSIQWEVSEQTVRKNRPSFAQTMLKVLVVFIAGLSLSVLLS
                     WPVIKNFNRIQEYALKYKPKAEVQPTANRIGKGNIDTYSAALDKDLAGIEKKLAVF"
BASE COUNT         1188 a          889 c          935 g          932 t
ORIGIN      
        1 gaggggaaat tcgagatggc cgacaaaggc actttattcc tggatgaagt tgccgacatg
       61 agcctgacac tccaggccaa gctgctccgc gttttacagg agggaacatt tgaaaggctg
      121 ggcagtaata aagtgatgaa agtagatgtc agggtcatct cagctaccaa taaaaatctc
      181 gaaaatgcca ttgccgggaa gaatttccgc gaagatcttt actaccggtt aaaggtcatc
      241 tccattacgc tgcccccgct ccgggtccat ccggaggata ttccccttct cacaaattat
      301 tttctgtcga ggcattgcaa ggagatgaaa aagcctcagg ttaccgtgcc gcctgaaacc
      361 atggaggctc tgctacgcca ttcctggcca gggaatgtca gggagctgga gaatatgctg
      421 aagcgggcac tgttgttaag taaaaacaat gtcatcacac ccgatcttgt gcatagcgag
      481 atccagtcga atgtccctgt taccaaagac caaccccatc cggcctatcc ttctctgatt
      541 ccggataaca tggaggattt cgagggtgtc ttgtttaaac atgtcatcga aaaggtcgaa
      601 aaggagataa taaacaaagc ccttgcgcat tgcaaaggaa accaggtaaa aacagcaaaa
      661 ttcctgggca tttcccgcgc catgctgatc gaacggatga aaaattacgg attgacgtaa
      721 tcctgcccat tgcccaacct gaattaaaaa ttaatgtttt cacgccagcc gcccgtcgtg
      781 attgttgatt ataacaacga tttatggccc cattgtcggc ggttggtgac ctggtcggcg
      841 gttgttgacc cggttggcgg ttgttgaccc ggttggcggt tgttgacccg tagagacgca
      901 aaattttgcg tctctacaaa aatggttcat cgaaataatg gttgcaatta taatatcggg
      961 gaaatggcgg aattgtttaa ttttcggtac agggtgccat cggcacgggc aaattggtgg
     1021 aattatggtg ataacggtgc ctattttatc accatatgta cgaaataccg ggtccattat
     1081 tttggcagaa ttataaatca tcaaatggat ctgtcgatca ttggtcaaca tgcatgggat
     1141 tgctggctgc aaatcccgga acattttcca tttgttgaat taggggcatt cgttgtaatg
     1201 ccgaatcaca tccacggtat tatcattata aacaaaaaga atattgagat acctgataag
     1261 agagataaga aaggtatggt gaccgatcat catgaaaatg ctgcaaaaaa ccaatttggc
     1321 gctcaatctc aaaatattcc atctattgta cgaggattaa agatcggtgt aaccaaattt
     1381 tgtaagcatg agaacctgcc atttggttgg caaccccgtt atcacgatca tatcattcgc
     1441 aatgcaaaag aatatgaaag aatcagcaac tacatattaa ataatcctgc aaaatggaaa
     1501 gaagatatcc acaatgccgg taacagatta ttgtaagaaa ttcagtcagc agtgtacaga
     1561 ttgtgatcac ttatttctaa ggacaagaat tttgattttt ataacagatc acatacctca
     1621 caaaatcaat attatacaac atttgagaaa tctttggcac gccatttgtt aagagggtgc
     1681 cgtcaaaaaa attgttgttt gaaatttaat gtttaacctt aataattaga aaaatgaaaa
     1741 gaaatgttct gttaacgatt gctgcacttg ctggaatgac ccttctgttc tccagctgcg
     1801 caaaacttcc cgaagctgaa atggccgctg ccaaagctgc tgtagaagct gctaaagctg
     1861 ctgaagctga ccgttatatt cctgctgaat acagggctgt taacgattct ctgaatgttg
     1921 ccatgaccga agttgagaac cagaaatcaa aattcgttct gttccgcagc tacaagaaag
     1981 taaccgttaa attaaacaac gttgttaccc tggccaacac ggtgaaagaa aacgccggta
     2041 tccgcaagga agaagtgaag aaccaggctc agcagaccct ggctgaatcg accaccctcg
     2101 tggaagaagt aaaggccctc atcctgaaag cccccaaagg caaggaaggt aaagaagctc
     2161 tcgaagctat ccagagtgac ctggcactgg ttgaagcatc actggccgag gtcagcaccc
     2221 tgatcaacaa tggcgattac ctgaccgctc tcgacaaagt aaaggctgcc aacgaaaagg
     2281 ctggttcact gaaagccgag ctggaagaag ctattgctaa aaaaaccatg aagaagtaat
     2341 tatcaaagat tttttcggcc gtaaaatttt aaatttgcac agaatttgac aagccggaaa
     2401 atacttaaaa ccgatcaaat atcgttttag ctggagaccc cggacatgca tacgcatccc
     2461 ggggtctttg gcgataaaaa taaaaggata tgaagcgaaa gcgcaaaatt aaactcagcc
     2521 gtgtactcat tgccctcggg gcattttgcg ctttgatcgc cctggtcttc ggcgtcctgc
     2581 tccttacttc ttccaaaccg ccggtttatg ccatcacgat ctgccagacg gagatcagcc
     2641 gggcacggga ggtcgaggcc gaccggtacg ctgccgagat gctgatcgat gcggaaagta
     2701 cctgccagct ggctatgaac gagtggaagt accagaatgc ccgcgtcttt tttgcccgcg
     2761 attacgacca gatgctcaac ctggctatgg cttcggccaa caaggcccgg catgcctccg
     2821 agcaggccct tcatatcaaa gattcacttc agtcggggct gagcgataaa ctgaaatcgg
     2881 tcagccataa aatcgatcac ttcgatgaat attatgccca tctgcctttg aacggaacca
     2941 ccaggcagaa tttcaccaca gctaaactcc gctatatgga gtcgaaagaa gcatacgaaa
     3001 ggggcgacta caagcaggtc ggggtaaatc ttgacgttgc cagccagctg ataacgcggt
     3061 cggtggccga agcgcataac tatctctccg attattttac aagcctctcc aaatggaagc
     3121 gctgggcaga tgaaactata gcctggtcga agaataataa tgcctatgct atccttgtcg
     3181 ataaatttgc gcataaatgc tatctttatc acgacggcaa ggtaaaaaaa gaatttacgg
     3241 ccgaactcgg gccccactgg atcgggacca agcaataccg cggcgataag gccacacccg
     3301 agggcaaata ccacgtaaca aagaagaaag cgaatcccca tacaaaatat tataaagccc
     3361 ttcttatcaa ttatccgaat gatgaagata aagcccggta tgaggccaat gtgagaaaag
     3421 ggaatatccc cagaaggggt attggcaacc tgatcgaaat acacggtgac gggggcaaag
     3481 ggatcaactg gaccgacgga tgcgtcgccc tgacaaattc cgatatggat aagctgtacg
     3541 ggctggtcgg cgtcggtaca ccggtaacga tcgtgggatc tttgagaagt ttgaaagaga
     3601 ttaacggatt ttaaccgata tttgcaacaa ataagcaagt caatggatga caggagtatc
     3661 cagtgggaag tcagcgaaca gactgtccgg aaaaatcgcc cctccttcgc gcagaccatg
     3721 ctgaaggtcc tggtggtgtt cattgcaggc ctcagcctgt cggtgctgct ttcctggccg
     3781 gtcatcaaga atttcaacag gatccaggaa tatgcgctga aatataagcc gaaagcggaa
     3841 gttcagccga ccgccaaccg gatcgggaaa ggaaatatcg acacctattc ggcggcgctt
     3901 gataaagacc tggcaggcat tgagaagaaa cttgccgtct ttat
//
LOCUS       DAOQXS010000287         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23556_length_3938_cov_4.129539, whole genome shotgun sequence.
ACCESSION   DAOQXS010000287 DAOQXS010000000
VERSION     DAOQXS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23556_length_3938_cov_4.129539"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKM34_08380"
     CDS             <1..187
                     /locus_tag="PKM34_08380"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNQ83646.1"
                     /translation="MLCIQVKEIMKLFSFEESRLEFAKYAYGYTYDLSNFYIVNDAFS
                     FESSIDELNKYIRSQNN"
     gene            283..1983
                     /locus_tag="PKM34_08385"
     CDS             283..1983
                     /locus_tag="PKM34_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNQ83647.1"
                     /translation="MNNRRVISIIKILIWIAPLLPWSLLPAQELMPVLTHEMTPEEEL
                     RKGEIGRSFTPTSPPLAPARAIAEFEQMQSVLIRYPFGIPMSLIVEMADDCMVTTIVA
                     SQAQLDQVLNQYIAAGVDTSHCEWLIAPTNSYWTRDYGPWFVADSSHQVGICDFPYNR
                     PRPYDDNIPVVLASQIDIPLYGMNLIHTGGNWMCEGLGNASSTELVWTENPTLTHQEI
                     DGLVESFLGIDTYHVLPDPLGEYIQHIDCWGKFLDVDKVLIGQVAESDYRYDDYEYVA
                     GYFASQISAYGTPYQVFRVYTPGDYPYTPYTNSLILNKKVLVPVTGSPWDDEALGAYA
                     DAMPGYEVIGVLYSGWQNTDALHCRAIGIADTGMLYIHHMPLSGEQEFSIGYTISCSI
                     IPYTDEELISDSLLCIYKIGNGSFQTVPLASGGGSVFSASLPYIAPGSQVSYYLHAAD
                     SSGRSMSHPFIGSPDPHVFTVQNEVPGLWTGEVSGDWMNPSNWSNNAVPASPTQVVIP
                     PGSSHWPVFSGDLVVGSTCGNIIITGTAQMTVTGSVTLHPGKTITILNNGHLIQTQTP
                     "
     gene            2134..2586
                     /locus_tag="PKM34_08390"
     CDS             2134..2586
                     /locus_tag="PKM34_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNQ83648.1"
                     /translation="MKNNLFKLLFTTIAIFAYVSLFAQAPADAITGKWLNEDKDAHIE
                     VYKESGKYFGKIVWLKDPIDEVTGKPKLDDENDDEALQSRPILGLVILKDFIYDGDGE
                     WEDGTIYDPKNGSTYDCYIQFGDDKTLKIRGYIGISWIGRNTYWTRVE"
     gene            2629..3573
                     /locus_tag="PKM34_08395"
     CDS             2629..3573
                     /locus_tag="PKM34_08395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNQ83649.1"
                     /translation="MNAIYAIGETVYDIIFRNDQPVAARPGGSMLNTAISLGRSGLKV
                     EMITELGDDPVGKLVTAFLNDNGVSTSFIQPAGNYKTPVSLAFLDDSGDARYSFYKNI
                     PEKRLNIGQAEAGKGDVVLFGSFYSLDAGIRHEIISFINKSKQNGALIFYDPNIRKNH
                     LGEINKLMPSVEENISLADIIRGSDEDFFNLFGLDDQFEIAHKVIQSGCHHMVITRGK
                     HGADYLSRDEAIHFPAIETKAVSTIGAGDAFNAGIIYAIIKNDIPGIELVNINSATWK
                     TILRIGTIFAAEVCKSYDNYIGKDFASQMNEENYIDTD"
     gene            3560..>3938
                     /locus_tag="PKM34_08400"
     CDS             3560..>3938
                     /locus_tag="PKM34_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83650.1"
                     /translation="MIQIRAGLYGFFVPFKDLYKSIPSNSLTFILFLFIPVFYIAASS
                     IAYNARAPYYLFSIDPEYFYMYNGVILGAGNLSIQYYAHPGTPLHFLVAISTRITDLF
                     QQGSYMENFVNDPEMFIRSANLFI"
BASE COUNT          997 a          994 c          913 g         1034 t
ORIGIN      
        1 tatgctctgc atccaggtca aagagatcat gaaacttttc agttttgaag agtcacggct
       61 cgaatttgcc aaatatgctt atggttacac ctacgacctg agcaatttct acatcgtgaa
      121 cgatgccttc tccttcgaat cgtcgatcga tgaactgaac aaatacatcc ggtcgcagaa
      181 taactgaggc aacgatttgc tttcagattg tatctttctt gatcagtgca accttgtccc
      241 tttccggtct gaattgcatg cattttaact acttttgccc tgatgaataa ccgcagggta
      301 atcagcataa taaaaatcct gatctggatc gcccctctct tgccgtggtc tttgctccct
      361 gcccaggaac tcatgcctgt cctgacgcat gaaatgaccc cggaagaaga gctgcgcaaa
      421 ggggaaatcg ggcggagctt tacgccgacc agcccacccc tggccccagc ccgggctatc
      481 gccgaattcg agcagatgca aagcgtgctc atccgctatc cgttcggcat cccgatgtcg
      541 ctgatcgtcg aaatggccga cgactgcatg gtgacgacca tcgtagccag ccaggcacaa
      601 cttgaccagg tgctgaacca gtatatcgcc gccggcgtcg atacatcgca ctgcgaatgg
      661 ctcatcgcgc caaccaattc gtactggacc cgcgactacg gcccgtggtt cgtagccgac
      721 agctcacacc aggtgggcat ttgcgacttc ccctacaacc gtccccgccc atacgacgat
      781 aatatccccg tggtgctggc ctcccagata gatatccctc tctatggcat gaacctcatc
      841 cataccggcg gcaactggat gtgcgaaggg ctgggaaacg cctcgtccac cgagctggtc
      901 tggaccgaaa atcctacgct cacacaccag gaaatcgacg gcctggtgga gagtttcctg
      961 ggcattgata cctaccatgt tctgcccgat ccgctgggag aatacatcca gcatatcgat
     1021 tgctggggga agttcctgga tgttgataaa gtactgatcg ggcaggtcgc cgaatcggat
     1081 taccggtacg atgattacga atatgtggcc ggctattttg cttcgcagat cagcgcttat
     1141 ggtacgcctt accaggtctt ccgggtctat acgccgggcg attatccata tacgccgtac
     1201 accaattcat tgatactgaa caaaaaggtt ctcgttccgg taaccggcag cccgtgggat
     1261 gatgaggcgc tcggcgccta cgcggatgcg atgccgggtt acgaggtgat cggcgtcctt
     1321 tattcgggat ggcaaaatac cgatgccctt cattgccggg caatcgggat agccgatacc
     1381 ggcatgctct atattcacca tatgcccctc tccggagagc aggaattcag cataggttat
     1441 acgatcagtt gcagtatcat cccatatacg gatgaggaat tgatttccga ttcgctgttg
     1501 tgtatttata aaatcggtaa cggttctttc cagacggtac cgcttgccag tggcggtggt
     1561 tccgtctttt cggcatcact cccttatatt gcgcccggaa gccaggtctc ctattacctt
     1621 catgccgccg actcttcggg ccgttcgatg agccatccct tcatcggatc tcctgatccg
     1681 catgttttta cggttcagaa tgaggtcccc ggcctgtgga ccggcgaagt gtcgggcgac
     1741 tggatgaatc cctccaactg gagcaataat gccgttcccg catctccgac tcaagtcgtt
     1801 atccctcccg gatcatccca ctggccggta ttcagcggtg acctggtggt cggctctacc
     1861 tgcggcaata tcatcataac cggaacggca caaatgacgg taacgggcag tgttacctta
     1921 catcccggaa aaaccatcac catccttaac aacggccacc tgatccaaac ccaaacacca
     1981 taatcccgta accctggagc cctgccatac tggcactcct acggagttca ctggatttta
     2041 aactttggaa ccctggaaca tatgaactca gggaccctgg aacattggaa catttaaaac
     2101 atttccctat atttgcctct taaaacccac cctatgaaaa acaatctttt taaactactt
     2161 tttacgacta ttgcgatttt tgcttatgta tcgctgtttg cccaggctcc tgccgatgcc
     2221 attaccggaa aatggctgaa tgaagacaag gatgcccata tcgaagttta taaggagagc
     2281 ggcaaatatt ttggtaaaat tgtctggctg aaagacccga tcgatgaggt gacggggaaa
     2341 ccgaagctgg atgacgaaaa cgacgatgaa gcgctgcaga gccgcccgat cctggggctg
     2401 gtgatcctga aggattttat ctacgacggc gatggagaat gggaagacgg aaccatttat
     2461 gaccctaaaa acggtagtac gtatgattgt tatattcagt ttggtgatga taagacgctg
     2521 aaaatcagag gttatatagg gatttcctgg atcggacgga atacttactg gacgagggtt
     2581 gaatgaggtc aatagatttt cgattttcga tttaggataa aatgtggcat gaatgcaata
     2641 tacgccatcg gtgaaaccgt ctacgatatt attttccgca atgatcagcc ggttgcagcc
     2701 cgaccgggcg gatcgatgct caatacagcc atttcactgg gacggagcgg attgaaggtg
     2761 gaaatgatta ccgaactggg cgatgacccg gtgggtaaac tggttactgc ttttttgaac
     2821 gataacgggg tttcaacatc cttcatccag cctgcaggta attataaaac ccccgtttca
     2881 cttgcgtttc ttgatgattc gggcgatgcc cgttattcct tctataaaaa tattccagag
     2941 aaaaggctga acatcggtca ggctgaggcc ggtaagggtg atgtggtcct tttcggatcg
     3001 ttttattcgc tcgacgccgg aatcagacat gaaatcattt cattcattaa taaatcgaaa
     3061 caaaacggtg ctttgatctt ctatgacccg aatatccgca aaaatcacct cggggagatc
     3121 aataaactca tgccttccgt ggaagaaaat atttcccttg ccgatataat ccgcggctcg
     3181 gatgaggatt tttttaacct tttcggcctt gacgatcaat ttgaaatagc tcataaagtg
     3241 attcaatcag gctgtcatca catggtcatt acccgcggga aacatggggc cgactatctt
     3301 tcgcgggatg aagcgattca ctttcctgcg atagaaacca aagcggtcag tacgatcggc
     3361 gcaggcgatg cgtttaatgc cggaatcatt tatgcaatta ttaaaaatga cattcccgga
     3421 atagaacttg ttaatatcaa ttcagcgacc tggaaaacaa ttctgagaat tggaactatt
     3481 tttgccgcag aagtttgtaa aagttatgat aattacatcg ggaaagattt tgcaagccaa
     3541 atgaatgaag aaaattacat tgatacagat tagagcaggc ctgtacggct tcttcgttcc
     3601 gtttaaggat ctttataaat ccattccatc aaattcttta acttttattc tattcctctt
     3661 tatccctgta ttttacattg cagcttcctc cattgcttac aatgcccggg ccccctatta
     3721 ccttttcagt atcgatcctg aatacttcta catgtacaac ggagtgatcc ttggagccgg
     3781 taacctgagt atccagtatt atgcccatcc cggtacccct ttgcattttc ttgtcgccat
     3841 ttcaacacgg atcaccgacc tttttcagca aggaagctac atggagaatt ttgtcaatga
     3901 tccggaaatg ttcatccgtt ctgcaaattt gtttatca
//
LOCUS       DAOQXS010000288         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23728_length_3918_cov_4.140047, whole genome shotgun sequence.
ACCESSION   DAOQXS010000288 DAOQXS010000000
VERSION     DAOQXS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23728_length_3918_cov_4.140047"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="PKM34_08405"
     CDS             complement(<1..2126)
                     /locus_tag="PKM34_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83651.1"
                     /translation="MTTYVKGNITAVVAALVFLFFADSYSYAQCRYSQEVNEEFRASG
                     ILRGPEHYYRNLIIQDIALARNVTPEEVSLEFHYNARFSVNRCTSGKLEITVAPPLFY
                     SPALFYHDFDISSYIRPSSGQMVFHLLQDKTIIADSIIFKNIIPNQDTGLYVSMDYTI
                     PSSVNDFQAVFSKARFFFDKSSYEQFRDQILHVDNFLAAVYVGRNLLDSIGINDRSGE
                     QPRGDLILAWIIMDKANRQLNPVNFRDLFVPVSHKSNALDSIARLLAIHQKRIGSILI
                     MNRKRPQGIISQVTSREELANLYFSHLDHYHDFAYSGDFRFVNFIEKLAEPVYSNAYL
                     LHLREFLAGQAGMDDAAIFSEGKRITGKMIRKGEELALAGDQLRAMKYFAASARLAGS
                     IRLYESFVYSRDKVAALKDSIAVSYVTISRKSASVNNPVLAVQYLEKAVNIYSNGIEA
                     KSQPGWLSEYRHELFRGFSLQIQALIKEENYSKCMEYLVQIQNYCRNYPGFICPDEFH
                     DQMKTVRQGIYKELLNRSRLMLAAEEYNEAEKLLNEAVELRTAGGYNIGREIEESQLE
                     LSFRQIEYDLNIEEGYRFSDLHEYNSTLYYFNKAFAAGKSGIGKTDPDLDKNRLNAAR
                     QVIRQVLSEGRVKAWANDYEGAEEIIIRIETMLNDYHVATNDSLYAELALLKESVSGS
                     KCRKVQEQYRQKITQADSLIKAGNFI"
     gene            complement(2179..2262)
                     /locus_tag="PKM34_08410"
     tRNA            complement(2179..2262)
                     /locus_tag="PKM34_08410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2226..2228),aa:Leu,seq:cag)
     gene            complement(2274..2357)
                     /locus_tag="PKM34_08415"
     tRNA            complement(2274..2357)
                     /locus_tag="PKM34_08415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2321..2323),aa:Leu,seq:gag)
     gene            2546..>3918
                     /locus_tag="PKM34_08420"
     CDS             2546..>3918
                     /locus_tag="PKM34_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNQ83652.1"
                     /translation="MDQIRARGISTDEIERQINDFKQGYPFAKLHAAATRENGIRTFN
                     EKEVHELITHFERTAKDMKLVKFVPASGAATRMFKHLYEFRERQEKVKKPGDILIDED
                     FNSVGYFFAHLNKFAFYDDLKRVMSRAGFNIEECIKRSDFDTVLDYLLTPAGLNYGNL
                     PKGVLKFHHYGTFSRTATGEHMVEGAFYAANRAKKVNIHLTVSPEHLSNFKDLIAYVQ
                     PYYESMFGVSYDVSFSVQKPSTDTIAVDLNNEPFREKDGSLVFRPAGHGALIENLNDL
                     DCDLVFIKNIDNIVPDRLKAETYRYKKLLGGLLLNLQVRSFQYLRALDKKPPTEETIA
                     EAVQFCEKQISISLPSGFEYWEESKKVGFLQQALNRPIRVCGMVRNEGEPGGGPFWVK
                     SKQGEISLQIVESSQIDMANPAQKKILQTATHFNPVDLVCSIRNYKGEKFDLRQARAF
                     LFVAEEL"
BASE COUNT         1108 a          907 c          895 g         1008 t
ORIGIN      
        1 tggataaaat tccctgcttt gatcagtgaa tcagcctgag tgatcttttg cctgtattgt
       61 tcctgtacct tacggcattt ggagccgcta acagattctt tgagcaatgc cagttccgca
      121 taaaggctgt cgtttgtggc tacatggtaa tcattcagca tggtctcaat gcggatgata
      181 atctcttcgg ccccttcgta gtcgttggcc caggctttaa cccggccttc cgaaagcacc
      241 tggcggatca cctggcgtgc cgcattcaaa cgattcttat ccagatcagg atctgttttg
      301 ccgatccctg attttccggc ggcgaaagct ttattaaaat agtacagagt cgagttgtat
      361 tcgtgcaggt ctgaaaaacg ataaccttct tcaatgttca gatcgtactc aatctgacgg
      421 aatgaaagct ccagttgtga ctcttcaatt tccctcccga tattgtaacc gccggcagtc
      481 ctcagctcaa cggcttcatt caacagtttt tccgcttcat tgtattcttc ggcagccaac
      541 atcaatctcg agcggttgag caactctttg taaatgccct gtctgaccgt cttcatttga
      601 tcatggaact catcagggca aataaatccg ggataattcc ggcaatagtt ctgtatttga
      661 acaaggtact ccatgcattt ggaataattt tcctctttga tcaatgcctg gatctgaagg
      721 gaaaagcctc ggaacaattc atgacggtat tcactcagcc atccgggctg cgacttcgcc
      781 tctattccgt tgctgtaaat atttactgct ttctccaggt attggaccgc cagtaccgga
      841 ttgttaaccg atgccgattt cctggaaata gtaacatacg aaacggcaat actgtccttt
      901 aatgcagcga ccttatccct ggaataaaca aatgattcat ataaacggat acttccggcc
      961 agccgggctg atgcagcaaa atatttcatc gcccttaatt gatcacccgc taaagccagt
     1021 tcttctccct ttctaatcat tttaccggtt attctttttc cttcgctaaa aatagctgca
     1081 tcgtccatgc cggcctgacc ggccaggaat tccctcaaat gaagcaagta agcgttacta
     1141 tagaccggtt cggcgagctt ttcgataaaa ttcacaaacc ggaaatcgcc ggaataagca
     1201 aaatcatgat agtgatccaa atggctgaaa tatagattgg ccagttcttc ccggcttgtt
     1261 acctgtgata ttatcccctg aggccttttc cggttcataa tcagaataga gccgatccgt
     1321 ttctggtgaa tggcgagaag ccgggcaatg gaatccagcg catttgactt gtgactgacc
     1381 ggaacaaaca gatcccggaa attaaccgga ttcaattgcc tgttagcctt atccattatg
     1441 atccaggcaa ggatcaggtc tccccttggt tgttcccctg agcggtcgtt aataccgatt
     1501 gaatccagca ggttgcggcc cacatataca gccgcaagga aattatcaac atgcagtatt
     1561 tgatcccgaa actgctcata ggaagattta tcgaaaaaga atctggcttt agagaaaacc
     1621 gcctgaaaat cattaaccga agaagggatg gtataatcca tagatacgta caagccggta
     1681 tcctggttgg gtatgatatt tttaaagata atactgtcgg cgataattgt tttatcctgg
     1741 agaaggtgaa aaaccatctg acctgatgag gggcggatat aggatgaaat atcaaaatca
     1801 tgataaaaca gagccgggga gtaaaaaagc gggggagcta ccgtaatttc aagttttccg
     1861 gatgtgcacc ggttaaccga aaagcgggcg ttgtagtgga attccagtga gacctcctcc
     1921 ggggtgacat ttcgggccag ggcgatatcc tgaatgatca gattgcggta ataatgttca
     1981 gggcctctca ggataccgga agcccggaat tcctcattta cttcttgaga gtagcggcat
     2041 tgtgcatagg aataggaatc agcaaagaat agaaaaacaa gggcagcgac gacggcggtg
     2101 atatttcctt ttacgtaggt tgtcaaaggt aaaggattaa attaagatgc cggcctttcg
     2161 gccggcatct gattcgagtg cccaagacaa gactcgaact tgcacgggcg ttcgcccact
     2221 acaccctgaa tgtagcgcgt ctaccagttc cgccacttgg gcattttgct cggtgcccag
     2281 aacaagactc gaacttgcac gaccgagcgg tcacatggcc ctcaaccatg cgcgtctacc
     2341 acttccgcca tctgggctaa aagcggctgc aaaattaata atttatttga atcctgaaga
     2401 tttatttaag aataatttca ttgaagataa aaagtaaaat atttgagatt attttcaagg
     2461 atcactattt ttacattttg aaagatgaag attaacttaa ccgatctctt atacaaagca
     2521 aatgcatatg tttaatcaga acgacttgga ccaaataaga gcccgtggca tttcaacaga
     2581 cgaaatagag cggcaaatca atgatttcaa acaggggtat ccatttgcaa aactccatgc
     2641 ggcagccacg cgtgagaatg gcatcagaac gtttaatgag aaagaggtgc atgaactcat
     2701 tacccacttt gagaggacag caaaagacat gaaactggtc aagtttgtcc ccgcctccgg
     2761 agcggctaca cgaatgttca aacacctgta tgaattccgc gagaggcagg aaaaagttaa
     2821 aaaaccggga gatatattaa tagacgaaga tttcaacagc gtcggttatt tctttgccca
     2881 tctgaataaa ttcgccttct atgatgatct taaacgggtg atgtcgagag caggatttaa
     2941 catcgaagaa tgtataaaac gaagcgactt cgatacagtt ctcgactatt tgctcacacc
     3001 ggcgggattg aactacggca acctgcccaa aggagtactc aaatttcatc attacggcac
     3061 attttcacgg acagcaaccg gggaacatat ggttgagggg gcattttacg ctgccaaccg
     3121 ggcgaaaaaa gtcaacatcc atctgaccgt ttcccccgaa catctttcaa acttcaaaga
     3181 cctgatcgct tacgtgcagc cctactatga aagcatgttc ggggtcagtt acgatgtcag
     3241 cttctcggtt cagaagccct cgacagatac catagccgtc gatcttaata atgagccttt
     3301 ccgtgaaaag gacggatcgc ttgtctttcg tcctgccgga cacggggcat tgatagaaaa
     3361 cctgaacgac cttgattgtg acctggtatt tattaaaaat atcgataaca ttgtccccga
     3421 ccgtttgaaa gcagagacat accgttataa aaagctactc ggcggtttgc tgctgaacct
     3481 ccaggtgaga tcattccagt atttaagggc acttgacaag aaaccgccta cggaagagac
     3541 gatagcggag gcggtgcagt tctgtgagaa acagatcagt atatcattgc catcaggctt
     3601 tgagtactgg gaagagagca aaaaggtcgg atttctccaa caggctttga acaggcctat
     3661 ccgggtatgc ggaatggtca gaaatgaagg agagcccggt ggcggcccat tctgggttaa
     3721 aagcaaacag ggggaaatct ccctccagat cgtggaatcc tcgcagatcg acatggctaa
     3781 tcccgcacaa aagaagatac tccagaccgc aacacacttt aacccggtcg acctggtatg
     3841 tagtatccgg aactacaaag gcgagaaatt cgacctgcgc caggcgcgtg ctttcctgtt
     3901 cgttgccgag gagttgca
//
LOCUS       DAOQXS010000289         3892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_23951_length_3892_cov_3.156112, whole genome shotgun sequence.
ACCESSION   DAOQXS010000289 DAOQXS010000000
VERSION     DAOQXS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23951_length_3892_cov_3.156112"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="hutI"
                     /locus_tag="PKM34_08425"
     CDS             complement(<1..1152)
                     /gene="hutI"
                     /locus_tag="PKM34_08425"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNQ83653.1"
                     /translation="MQILVKNIRELVQVEVDPVKLKAGREMTGLNTIRNAYLVISNGR
                     IDSFGPMEGLAGKLGGYAGRWHRILDARGKMIFPAWCDSHTHVVFPASREIEYVDKIK
                     GLSYEEIAKRGGGILNSARRLHDTSEKKLFEDAMERIGEMISYGTGAIEIKSGYGLNP
                     EDEMKMLRVIRKLKEKSPATIIATFLGAHAVPEEYKGNRSAYVDVVINEMIPRVAGEE
                     LADFIDVFCDRGFFTVEDTDRILNAGMKYGLRPKIHANELGITGGVQVGVKYNALSVD
                     HLEHIGDEEIAALKDSETMPTVLPGASFFLGLPYSPVRKMIDAGLPVAMASDHNPGSS
                     PSGNMQFILSMGCIKYRMLPEEAINAVTLNGAYAMGLEEELGSIALGKEA"
     gene            1426..3534
                     /locus_tag="PKM34_08430"
     CDS             1426..3534
                     /locus_tag="PKM34_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNQ83654.1"
                     /translation="MKLKITFLALIALCTGLNAGAKQVSIDEAEKIARNFIYITINKY
                     DDGISFDRITLADPYIHQVDGKAVFYAFQMNPGFIIVSADDLFTPVIGYSFNGRFDLA
                     GASPNYRGFILNYADQITFARQNNVPASAEVNAAWAELRTDQIEAVSVTRDRDVEPLV
                     SSLWDQGDPYNYYCPADAAGPGGHTWVGCVATAMAQIMYYWRYPLNGTGDHCYIPGNY
                     SYGEQCADYANTYYQWDGMINSVDDKNPYGNAELQYHCAVSVNMNFNPNGSGAQSSNV
                     PNALNQYFRYNSAQYREKGNYTLSGWIALLKADIDVGKPVYYSGYSPSEGGHAFVCDG
                     YQGDNFHFNFGWSGYGNGYYSLSDVFGFYQGQACVRNFVPSDINYPYINTGSITVTNT
                     SGSITDGSGPVEDYTNNINAFWLIDPQSEYDSINNITISFSQFDLGSGDSVKVFNGES
                     INSPLLGAYGGTNMPPSLTTTGNKMLVQFKTNGSGTGSGWYAEYSTTSPTWCQGLVQY
                     TEPTGTFDDGSGEDFYYQNGATCMWRIKPPYANKIILTFNYFDTEEGIDKLNVYDGTT
                     LLAELSGNELPDPIEATSGSIFMTWSTNINTTRPGWEIYYEVDNIGISENSPVTELSV
                     FPNPANDKLHVEFNINSTEDLKIKLVNITGQTLYSETHPGFSGNYQNEIAVGSLPAGM
                     YFLEINAVTGTANKKIFINR"
BASE COUNT          996 a         1000 c          874 g         1022 t
ORIGIN      
        1 agcttcttta cccagggcaa tgctccccag ttcctcttcc agtcccatgg cgtacgcgcc
       61 gttcagggtg accgcattga tggcctcttc cggcagcatc ctgtatttaa tacatcccat
      121 ggaaaggatg aactgcatat tgcccgatgg cgatgatccg ggattgtggt cgctggccat
      181 agccaccggc agaccggcat cgatcatttt acgcaccggg gaatagggca atcccaggaa
      241 aaagctggcc ccgggcaaaa cggtcggcat ggtctctgaa tcttttagtg cagcaatctc
      301 ctcatcgcca atatgttcca ggtggtccac agaaagggca ttgtacttaa cccccacctg
      361 taccccgcct gtgatgccca gttcattggc atggatcttc ggccgcaggc cgtatttcat
      421 acccgcattc aatatacggt cggtatcctc cacggtaaag aaacccctgt cgcagaaaac
      481 atcgatgaag tcggccagtt cttcgccggc cacccgggga atcatctcat tgatcaccac
      541 atcgacataa gcagaccggt tgcctttata ctcttcggga acggcgtggg caccgaggaa
      601 agtagctata atagtcgccg gcgatttctc tttcagcttg cggatgaccc ggagcatttt
      661 catctcatct tccggattaa ggccataacc gctcttgatc tcgatagcgc ctgtgccata
      721 ggagatcatt tccccgatac gttccatagc atcttcgaac agcttcttct cagatgtatc
      781 atgcagtctg cgggccgaat tcaggatccc tccgcccctt tttgctattt cttcgtaaga
      841 taatcctttg atcttatcga catattcgat ttcacggctg gccggaaaaa cgacatgagt
      901 gtgagagtcg caccatgccg ggaagatcat tttcccccgg gcatcgagga tccggtgcca
      961 tcggccggca tatccgccaa gtttcccggc cagtccttcc atcggcccga acgaatcgat
     1021 ccggccatta ctgatgacca gataagcgtt ccggatggta ttcagaccgg tcatctccct
     1081 gcctgctttc agcttcaccg ggtcgacctc cacctgaacg agttccctga tattttttac
     1141 cagtatttgc atcgtcattt taaactatgc tgcgaaagta ataaaaaggc taaaaattat
     1201 tttcttcatg caaccttttc acacagtttc ctgtcatatt aaaagcaaat cggttgttct
     1261 ctgatttggt tttggtgaac taatttggtt tatttaatgg ttgtcgggca cctgtcgtaa
     1321 aaaagcaggt gcttcttttt tataacaaat aagttactgt aaaatatcct gcaaattcaa
     1381 gaattagtta tctttgcaac cttcattcaa atcaacatct tacatatgaa attaaaaatt
     1441 acattccttg cacttattgc gctctgcacc ggattaaacg caggggccaa acaagtgagt
     1501 atcgacgaag ccgagaaaat cgccaggaat ttcatttaca tcacaatcaa caagtatgat
     1561 gatggaattt cttttgaccg gatcacattg gctgatccat atattcacca ggtggatggc
     1621 aaagctgtgt tctatgcatt ccagatgaat ccagggttta tcattgtttc ggcggatgat
     1681 ctgttcaccc cggttatcgg ttattctttt aatggccggt ttgacctggc aggagcttct
     1741 ccgaattacc ggggattcat tctgaattac gccgatcaga tcacctttgc acggcagaac
     1801 aatgtccctg cgtcggccga agttaacgca gcctgggcgg aacttcgcac agatcagatc
     1861 gaagcggtat ccgttacccg cgaccgggat gtcgaaccgc tggtatccag tttgtgggac
     1921 cagggcgatc cgtataatta ttattgccct gccgatgctg caggtccggg aggacatacc
     1981 tgggtaggct gcgtagctac tgctatggcc cagatcatgt attactggcg ttatcctctc
     2041 aatggtacgg gtgaccactg ctatatcccc ggcaactact cctacggcga acagtgtgcc
     2101 gattatgcca acacttacta ccaatgggac ggtatgatca acagcgtcga cgataaaaat
     2161 ccttacggaa atgccgaact gcaataccat tgcgcggttt cggtcaatat gaacttcaac
     2221 cccaatggat ccggagcaca gagttccaat gtgcccaatg cacttaacca gtatttccgt
     2281 tacaacagcg cacaatacag ggaaaaagga aactatacgc tttcgggctg gattgccttg
     2341 ctgaaagccg atatcgatgt tggcaaaccg gtttactatt cgggatacag ccccagtgaa
     2401 ggaggccatg cttttgtttg cgacggttac cagggcgata acttccactt taatttcggc
     2461 tggagcggtt atggaaacgg ttattattcc ctgtcggatg ttttcggatt ctaccagggc
     2521 caggcctgcg tcagaaactt tgtcccttcg gatatcaact acccgtatat taacaccggc
     2581 agtataacgg tcaccaacac ctccggatcg attaccgacg gcagcggccc cgtggaagat
     2641 tacaccaata atatcaacgc tttctggctc attgacccgc agtcggaata cgattctatt
     2701 aataacatta cgatttcctt ttcacagttt gacctgggat caggagactc cgttaaagtc
     2761 tttaacgggg aatccatcaa ttccccgttg ctgggagctt atggcggaac taacatgcct
     2821 ccgtcattga ctactaccgg caataagatg ctggtgcagt tcaagaccaa cggaagcggt
     2881 accggaagcg gctggtatgc cgaatattcc actacttctc ccacgtggtg ccagggtttg
     2941 gtacagtata cagaacccac gggtacattt gacgacggca gcggcgaaga cttctattat
     3001 cagaacggcg ccacctgtat gtggaggatc aaaccgccct atgccaacaa gatcatcctc
     3061 acattcaact acttcgatac tgaagaagga atcgacaaac tgaacgttta tgacggcacg
     3121 acactgctgg ctgaactttc cggcaacgaa ctgcctgatc cgatcgaagc cacatccgga
     3181 agtattttca tgacctggag caccaatatc aataccaccc gccccggttg ggaaatctat
     3241 tatgaagtcg ataatattgg tatatcggaa aattccccgg ttaccgaact gagtgttttc
     3301 ccgaacccgg ccaacgataa gcttcatgtg gaattcaata taaacagcac agaagacctg
     3361 aaaatcaaac tggtcaacat tacgggccag actctttatt cagaaacaca tccgggcttc
     3421 agcggaaatt atcagaatga gatcgccgtc ggttccctgc cggcaggcat gtatttcctc
     3481 gaaattaatg ccgttaccgg cacggccaat aagaaaatct tcattaacag gtaatactga
     3541 gatttagaat tcattttctc tgaaaagagg ctatctgaaa agtcagatca gcctcttttt
     3601 tttatactca ttctttgcag gtttgcctca gcaaagctgc ttagaatgag ttgtgccggg
     3661 gagaacatct gttaaaattc gcaaaacagt tcagactcgg tataaacttc tttgtacatt
     3721 tatatcgtaa aatgaccagg atgaaaaagc tattactctt acccgccgcc attcttattc
     3781 ccatcttatg tatggcgcag ggcaggttca tcactcccgg tgaagccggc gctgttgctg
     3841 taaaatggta tatcgggcac atgcccggtc agcgctttac cgatccttcg ga
//
LOCUS       DAOQXS010000290         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24058_length_3882_cov_3.478181, whole genome shotgun sequence.
ACCESSION   DAOQXS010000290 DAOQXS010000000
VERSION     DAOQXS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24058_length_3882_cov_3.478181"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="PKM34_08435"
     CDS             complement(<1..2508)
                     /locus_tag="PKM34_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ83655.1"
                     /translation="MRYLFTLSVFCILFIFQSRAQNVSPGISDYSSYPYWIEMMSDAS
                     VNFYDVQEAFNTYYEGKDLTKLKGWKPFKRWEYSMLQGRIFPDGTRRPEDHIVKAYES
                     YLATHPGARSQAGDWTNLGPFVLPDGKGYKGLGRLNAIAFDPEDPDIVWVGSPSGGLW
                     KTTTHGGEWTSSTQELPSLGVSAILIDPTNPDVMYIGTGDRDAGDAYGIGVYRSTDGG
                     DTWEPWNLGMTNYVVGRLLMHPSNHMIIFAATSGGLFRTTNGGATWTRVQTGDFKDVV
                     FKPNNPDIVYAAANGNFYRSSNNGLNWTQITSGLPGSSRSAIAVTPDNPNYVYVILTY
                     GDSFMGLYRSTDAGLNFTMMSNSPNIMSWGCNGGDGGQAWYDLDIACDPNDADVIFAG
                     GVNCFKSVTGGSIWSISSHWWGDCGVPSVHADLHVLEYNPLNDRLYAGNDGGVYYTDD
                     QGETWTEITNGLPIGQEYKIGQSATMRDKTVNGYQDNGTSTYMGNYWQFILGGDGMEC
                     AVDPYDASYSYATVYFGYIARYYNNNSNGTVAENGFNGINESGAWVTPFILDEKNSNI
                     MFVGYKNIWRSTNIKAPSSQIAWQKISSNLGGSDSDDMRVIEQSPVNTDILYAARYDN
                     ELFRSDNCKAATPNWFTLTNLLPQSGGIYDIECDPFDENIVYLSQNNKIYKSIDKGQT
                     WEDISGTLPGIAFTSIAAYRNSHGGLYISSDIGVFYKDDFMTDWIMFSDGLPADASLN
                     EIEIYYDADPALDILRAGSYGRGLWESDVYHAAPDANFEARETTVPPGCEVDFNDLSS
                     GIPTEWLWEFEGATPSTSTERNPVGIIYENPGSYQVKL"
     gene            complement(2615..3472)
                     /gene="cyoE"
                     /locus_tag="PKM34_08440"
     CDS             complement(2615..3472)
                     /gene="cyoE"
                     /locus_tag="PKM34_08440"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNQ83656.1"
                     /translation="MPLINSYLELGKVKITFAVALTTITGYLLGGGSFDSGLILPTLG
                     IFLLACGSSAINHYQERERDALMERTRHRPIPSGRISPGAALAFAFLMSMAGSGLLYI
                     GSGILAMQLGILALIWYNVIYTPLKKKSAFAVVPGAVIGAIPPLVGWVAAGGSLTDPR
                     AMFMAFFFFIWQVPHFWLLMLKYGEEYTKAGYPSITSVYSSRQIKHITFIWILATAVS
                     TLMLPAFNVVESWVVMGGLLISSMWLVGQFTRLLSLKEMPFSPIRYFMKINYFVLAVI
                     IFLSIDRFI"
     gene            complement(3563..>3882)
                     /locus_tag="PKM34_08445"
     CDS             complement(3563..>3882)
                     /locus_tag="PKM34_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNQ83657.1"
                     /translation="VQSDIPADEHPGLSVLKQNGCIACHSLDGSKIVGPSFKGIYGHE
                     VEVITDGKERKIIVDDEYILKSIYEPNADVVKGFNPGLMISYKEQLKEEDVKKIIEYI
                     KGL"
BASE COUNT         1009 a          976 c          919 g          978 t
ORIGIN      
        1 cagtttcacc tggtagctgc cgggattctc gtaaatgatg ccaacaggat tcctttcagt
       61 tgaagtagaa ggagtagctc cttcaaactc ccatagccat tcggtcggga tccctgaaga
      121 aaggtcattg aaatcaactt cacagcctgg aggaacggtg gtttcacggg cttcaaaatt
      181 ggcatcgggt gcggcatgat aaacatccga ttcccataac ccacggccat aggatccggc
      241 tcgaaggata tccagggcag gatcagcatc ataatagatt tctatctcgt tcagtgatgc
      301 atcggccggc aatccgtcgg aaaacatgat ccagtcggtc atgaaatcat ctttataaaa
      361 aacaccaata tcggagctga tataaagccc gccgtggctg ttcctgtatg cggcaatgct
      421 tgtaaaggca atgccgggca gcgttccgga gatatcttcc caggtctggc ctttgtcgat
      481 cgacttataa attttattat tttgtgataa gtatacgata ttctcatcaa aaggatcaca
      541 ttcgatatca taaataccac cgctttgtgg cagtaaattg gtcagtgtaa accagttggg
      601 tgtggctgct ttgcagttat cagagcggaa aagctcattg tcgtaccggg ctgcatagag
      661 gatatccgtg ttgaccggtg attgctcaat gacacgcatg tcgtcactat cacttccccc
      721 caggttactg ctgatttttt gccaggcaat ctggctgctg ggtgctttga tgtttgtaga
      781 tctccagatg tttttatatc cgacgaacat gatattggaa ttcttttcat ccaggatgaa
      841 aggggtaacc caggctccgg actcattgat gccattaaac ccgttctctg ctacggttcc
      901 gttgctattg ttattgtaat accgggcaat gtagccgaaa tacacagtag catagctata
      961 gctggcatca taagggtcta cggcgcattc cattccgtcg ccgcccagga tgaactgcca
     1021 gtagttaccc atgtaggtcg atgtgccatt gtcctggtac ccgttgaccg ttttatcgcg
     1081 catcgtggcg ctttgcccga tcttgtattc ctgtccgata gggaggccat tggtaatttc
     1141 agtccatgtt tcaccctggt cgtctgtgta gtaaacccca ccgtcattac cagcatacag
     1201 cctgtcgttc agtggattgt attcgagcac atgcaaatcg gcatgcactg acggaacccc
     1261 gcagtcgccc caccaatggg agctgatcga ccagatcgat ccgccggtaa ccgatttaaa
     1321 gcaattgact ccaccggcaa agatcacatc ggcatcattc ggatcgcagg caatgtcgag
     1381 gtcgtaccag gcttgtccgc catcgccgcc gttacagccc caactcataa tgttagggga
     1441 attcgacatc atggtgaaat tcaggccggc gtcggttgac cggtacaaac ccatgaagga
     1501 atcaccgtat gtcaggatta cataaacgta attaggattg tcgggggtaa cggcaatagc
     1561 ggatcgcgaa cttcccggaa ggccgctggt aatctgggtc cagttcagcc cgttgttgga
     1621 agaacggtaa aaattgccgt tggccgcggc gtaaacgata tcagggttat tcggtttaaa
     1681 gaccacatct ttgaaatcgc ccgtctgcac ccgggtccag gtagctccgc cattggtggt
     1741 gcggaatagt cctccgctcg ttgcggcaaa gatgatcata tggttagagg gatgcatcag
     1801 gagccgtccg acaacgtaat tcgtcattcc caggttccat ggttcccatg tgtccccgcc
     1861 gtccgtcgaa cgatacacgc caattccgta agcatcgccg gcatcccggt cgcctgtgcc
     1921 gatgtacatc acatccggat tggtcggatc gatcagaatg gctgatacac ccagcgaagg
     1981 cagttcctgt gtcgaagagg tccattcacc gccatgcgtg gtggttttcc ataagccgcc
     2041 ggagggcgaa cccacccaaa cgatatcagg atcttccggg tcaaaagcga tggcgtttaa
     2101 ccttccaagc cctttatagc ctttgccgtc aggaagtacg aaaggtccca ggttggtcca
     2161 gtcgccggcc tgtgaccggg ctcccggatg ggttgccagg tatgattcat aagccttaac
     2221 gatatgatcc tcaggtcttc gtgttccgtc gggaaatatc cggccctgga gcatgctgta
     2281 ttcccacctt ttaaagggtt tccaaccctt cagcttggtc aggtcttttc cttcataata
     2341 cgtattaaat gcctcctgaa catcataaaa attcacagac gcatccgaca tcatttcgat
     2401 ccagtacgga tagctggagt aatcggatat accgggagaa acattttgtg cacgtgattg
     2461 aaaaataaac aggatgcaaa aaacggaaag agtgaataaa tatctcatct tcattaatat
     2521 aaatcaaaat ataggcaaat gccggattcc aaaaatacac atttttaatt actgaatgta
     2581 tttaaggtta aaaaacgttc ggaaaaaaaa cggtttagat gaaccggtcg atactgagaa
     2641 agataatcac agcaagtacg aaataattga ttttcataaa atagcggatg gggctgaaag
     2701 gcatctcttt cagggaaagc agccgggtaa actgtccgac cagccacatg gaagagatca
     2761 ataatccccc cataacgacc catgattcga cgacattaaa agcggggagc atcagggtgg
     2821 aaacggctgt ggccagaatc cagataaaag tgatatgttt aatttgccgg gatgaataca
     2881 ctgacgtgat ggatggatag ccggctttgg tgtactcttc gccgtatttt agcatcagga
     2941 gccagaagtg ggggacctgc cagatgaaaa agaagaaggc catgaacatc gccctgggat
     3001 ccgtaaggct tccgccggct gcaacccagc cgaccagggg aggtattgcg ccgatgaccg
     3061 ctcccggaac tactgcaaaa gcgctctttt tcttcagagg agtgtaaatg acgttgtacc
     3121 agatgagcgc caggataccc aattgcatgg ccagtatgcc cgaaccgatg taaagcaaac
     3181 cagagccggc catcgacata agaaaagcaa aagctaatgc agcccccgga gagatccttc
     3241 ctgaagggat cgggcggtga cgggtccgct ccatcagggc gtcacgttct ctctcctggt
     3301 agtgattgat ggccgacgaa ccgcaggcca gcagaaaaat acccaatgtc ggcaaaatca
     3361 atcccgaatc aaacgacccg ccacccagca ggtaccccgt gatcgtcgtc agcgccaccg
     3421 caaacgtgat ctttaccttt cccagctcaa gatatgaatt gatcagtggc attgttcaat
     3481 tgttcaattg ttctattgtt atttttttcc agtctgaccg tcctactgtc ttaacatctt
     3541 accgtcatac cgtcataccg tcctaaagtc ctttaatata ctcaattatc ttcttcacat
     3601 cctcctcttt caactgttct ttgtaggaga tcatcagccc cggattgaat ccttttacca
     3661 catctgcatt cggctcatag atcgatttta gtatgtattc atcatcaaca ataatcttcc
     3721 tctcttttcc gtcggtaatc acctcaacct catgcccgta tatacctttg aacgaagggc
     3781 ctacgatctt actgccgtcg agtgagtggc aggcaataca accattctgc ttcaaaacgc
     3841 tcaaccccgg atgttcatcg gcagggatat ccgattgaac ca
//
LOCUS       DAOQXS010000291         3879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24088_length_3879_cov_3.242678, whole genome shotgun sequence.
ACCESSION   DAOQXS010000291 DAOQXS010000000
VERSION     DAOQXS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24088_length_3879_cov_3.242678"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKM34_08450"
     CDS             <1..344
                     /locus_tag="PKM34_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83658.1"
                     /translation="GSAIKHFSVKWNFFTRFTLQGPVSIDENEPFAVISLDIFPNPVE
                     GLATVDISGLESDGFIFRLMDINGRVCKEQNNFREKRFLLDASLLPAGLYLAQIVQDR
                     NIVVSTKLMIR"
     gene            complement(345..1370)
                     /locus_tag="PKM34_08455"
     CDS             complement(345..1370)
                     /locus_tag="PKM34_08455"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNQ83659.1"
                     /translation="MSGVIDLRSDTVTRPSPAMLEYMFNAEVGDDVLGDDPTVIKLES
                     RIAGMFGKEDALFCPSGTMTNQIAVKVHTQPGDEVICESTSHVYRFEAGGMAFNSGCS
                     VRLIQGDRGRLKPHQVLENLNPDNVHYPQTRLVVAENTSNMGGGSIYDYADLVELSAT
                     CKEKNLRFHLDGARIFNALVETGQKPEDYGRLFDSVSVCFSKGLGAPVGSALIGSREF
                     ITKARRIRKVFGGGMRQSGYLAAAAIFALENNITRLKEDHRRAKVLSGHLAGMSYVEE
                     VYPAETNIIIFKLNSKYTQPQLLRLFEDRSLWAVGFGHDKIRLVTHFDFDDQLLARSL
                     EVFSSID"
     gene            1439..2011
                     /locus_tag="PKM34_08460"
     CDS             1439..2011
                     /locus_tag="PKM34_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83660.1"
                     /translation="MKSGKSHLYVLAVIVLVITACHEEWKTIVVAKGNHSSNEISPML
                     FQTDRIDFYFIADSSWYYPPPVSPGWNKIRGLSHGHHQNNSSARLVYQCIGNTLLVVG
                     AYCYADGISPQQNPALKGIIDTIQPGIKYFCSIHRKDDQYIFKFEDKTWIGPAGKYLS
                     WGYLLNPYIGGEFTLEKDWVVKIKDEKISE"
     gene            2096..2473
                     /locus_tag="PKM34_08465"
     CDS             2096..2473
                     /locus_tag="PKM34_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83661.1"
                     /translation="MAIIDAVIKLRAKYKPVVKDSKWRLFAMIVLHVQFIIGLILYFI
                     SPKVIFDAASMKNSMQRFFLVEHIALMIIAIVLITVGHVKSKRAVDNIRKQKLVIVYY
                     GIGLLLILLSIPWPFRNFGAGWL"
     gene            2618..>3879
                     /locus_tag="PKM34_08470"
     CDS             2618..>3879
                     /locus_tag="PKM34_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83662.1"
                     /translation="MKKLVILLIISVLTGTGYLGAQDQPIQPSIVKTGVYHGISPALR
                     DLPAITDAEFQQLVIKSEQKMLNKKLRERHYPYASTAQPQGPDPVWQDQMGKTPGMKA
                     PIMNFSGQTSPYYPPDANGSAGPNHYMQTINTVYTIYNKSTGALVAGPTNLNLLFSGV
                     TGAEYNDGDPICLYDEQADRWLVAEFSISGSNDYMLIAVSTTNDPTGTWHKYSFDVAD
                     MPDYEKFGIWQDGYYMGTNNSTGNDIYVFERSKMLDGLTAQMVGFNNPWRPTTIDGFM
                     CVPPVDNDGTFAPAGSPGLFITINDDAIGGGTDQLWIYELAVNWTTPSNSTFTRSQQI
                     NVPAFDSNFGSNWDNIKQPNTTRELDAIPMVIMNVPQYRNFGSYQTIVCCHTVDVDNT
                     DHAGIRWYELRRTTGTWTVRQSGTYAPDA"
BASE COUNT         1062 a          922 c          864 g         1031 t
ORIGIN      
        1 aaggaagtgc catcaagcac ttttccgtca agtggaactt tttcacccgg tttacccttc
       61 aaggaccagt gagtatcgat gaaaatgaac cctttgcggt aatcagcctg gatatttttc
      121 ctaatcctgt ggaaggcctg gctacggttg atatttccgg tttggagtct gatgggttca
      181 ttttcaggct gatggatata aatgggagag tctgcaagga gcagaacaat ttccgtgaaa
      241 aaagattctt gctggatgct tccttacttc ctgccggatt atatctggct caaattgtgc
      301 aagatagaaa tattgttgtc agcaccaagc tgatgatcag gtaatcaatc tatactgctg
      361 aaaacctcca ggcttcgggc gagtaattgg tcatcgaagt cgaagtgtgt taccagccgg
      421 atcttatcat ggccgaaacc cacagcccat agcgaccggt cttcaaacaa acgcagcaac
      481 tggggctgtg tgtatttgct gtttagcttg aaaatgatga tgttcgtttc ggcaggataa
      541 acttcttcaa cataactcat accggccaga tgccccgaaa gtaccttagc gcgcctgtga
      601 tcctctttta accgggtaat attgttttcc agtgcaaata ttgccgcggc ggccaggtag
      661 cccgattgcc tcatcccgcc accgaatact tttctgattc tcctcgcttt tgtgatgaat
      721 tccctgcttc cgattaaagc cgatccgacc ggtgccccca atccctttga aaaacaaaca
      781 gaaacagaat caaataatct tccgtaatct tccggcttct gacccgtctc caccaaagca
      841 ttgaatatcc gggctccgtc gaggtgaaac ctgaggttct tttctttgca agttgcgcta
      901 agttcaacca ggtcagcgta atcgtaaatg ctcccgccgc ccatgttgga tgtgttttca
      961 gcaactacca gccgggtttg aggataatgg acattatcag ggttcaggtt ttccaatacc
     1021 tgatggggtt tgagccgacc gcggtcaccc tggatcagtc gtacggaaca acctgaatta
     1081 aaggccattc ctccagcctc aaaccggtat acatgtgaag ttgattcgca gatcacttca
     1141 tcgcccggtt gtgtgtgaac tttaactgca atttgattgg tcatggtgcc ggagggacaa
     1201 aaaagcgcat cttctttgcc aaacataccg gcaatccgtg attccagctt aataaccgtc
     1261 ggatcatcgc ccaggacatc atctccgact tccgcattga acatgtattc aagcatggca
     1321 ggtgaggggc gcgttacagt atcgcttcgt aaatctatta cgccagacat attatctttg
     1381 tttaaaatca ggttataaaa ttataaatgt atgatgaggt aataccaaat taatcgaaat
     1441 gaaatcagga aaaagccatt tatatgtatt agcggtaatc gttttagtaa taacagcctg
     1501 tcacgaagaa tggaagacca ttgttgtcgc taaaggaaac cactcttcca atgaaatcag
     1561 cccgatgcta tttcaaaccg acagaattga cttttatttt atagccgatt ccagttggta
     1621 ttatccgcca cctgtttctc caggatggaa taaaatacgt ggtttgagcc atggtcatca
     1681 tcagaataac tcttcggcaa ggcttgtata ccaatgtatc ggcaacacgc tgctggttgt
     1741 cggcgcatat tgttatgctg atggaatcag cccgcagcag aatcctgcac ttaaaggaat
     1801 aatcgacaca attcagcctg gaattaagta cttttgcagt atacacagaa aggacgatca
     1861 atacatattt aagtttgaag ataaaacctg gattggcccg gctggaaagt atttatcatg
     1921 gggctacctg ctgaatccat atatcggcgg ggaatttacg ctggaaaaag actgggttgt
     1981 gaaaatcaaa gatgaaaaaa tcagtgaata acgaaaatct gaagccatga acctatatcc
     2041 catactgata catttgcaca gcatttttcg ctgggtagtg cttatattga ttgttatggc
     2101 cataattgat gcggtcataa agctgagagc gaaatacaaa cccgttgtta aagacagcaa
     2161 gtggagatta ttcgccatga tcgtcctgca cgttcagttt atcatcgggc tgatactcta
     2221 ttttattagt cctaaagtca tttttgatgc tgcctccatg aaaaacagca tgcagcgttt
     2281 tttcctggtc gaacacattg ccctgatgat aatcgcaatt gtgttgataa cagtagggca
     2341 tgtaaaatcc aaacgcgcgg tcgacaatat cagaaagcaa aagttggtca tagtctatta
     2401 cggaatcggt ttgctattga tcctgctttc cattccatgg cctttccgga actttggcgc
     2461 cggctggcta tagaaatctt ttttttgcat tctttccccg gaaccgaata acctttaccg
     2521 gtctttggtg attaatcatg tcgtgttgca accatctgca gggtgcaccg taaaagttta
     2581 aatcacttat gcaataacct taaaccaaaa atcaattatg aaaaagcttg taattttatt
     2641 aattatcagc gttttgacag gcacaggtta tcttggggca caagaccaac ccatccagcc
     2701 atccatcgtg aaaaccggtg tttaccatgg gatttcgcct gctttgcggg atcttccggc
     2761 tataacagat gcagagtttc agcagcttgt aattaagagc gagcagaaaa tgttgaacaa
     2821 aaaacttcgc gagcggcatt acccatatgc gagcaccgct cagccacagg ggcctgatcc
     2881 ggtctggcag gaccagatgg gcaaaacgcc tggaatgaaa gccccgatca tgaatttcag
     2941 tggtcagacg tcgccctatt atccgcctga tgccaatggc tcagccggac cgaaccatta
     3001 catgcagacg atcaatactg tgtataccat atataataaa tctaccggag ccctggttgc
     3061 cgggcccacc aatctgaacc tgcttttcag cggcgtgacc ggtgcggaat ataatgacgg
     3121 ggatccgatc tgtttgtatg acgaacaagc cgaccgctgg ctggttgccg agttctccat
     3181 cagcggatcc aacgactata tgctgatcgc cgtttctacc accaacgacc cgacagggac
     3241 ctggcacaaa tactctttcg atgtcgccga tatgccagac tacgagaaat tcggcatctg
     3301 gcaggacggt tattacatgg gaaccaataa cagtaccggt aacgatattt atgtttttga
     3361 aaggtcgaaa atgctggatg gactcaccgc acaaatggtc ggttttaaca acccctggag
     3421 gcccaccacg attgacggtt ttatgtgcgt tcctccggta gataacgacg gcacatttgc
     3481 tccggccggc tcacccggat tgttcatcac cattaatgac gatgctatcg gcggtggtac
     3541 cgaccagctc tggatttacg aactggcagt caactggacc acaccatcaa attccacatt
     3601 cacccggagc cagcaaatca atgtgcctgc attcgatagc aatttcggca gcaactggga
     3661 taatatcaaa caacctaaca caacccgtga actcgacgcc atacccatgg tgatcatgaa
     3721 tgttccgcaa taccggaact ttggatccta ccagacgatc gtttgctgcc atacggtcga
     3781 tgtcgacaat accgatcatg ccggtattcg ctggtatgaa ctccgccgga caactggcac
     3841 ctggactgtc agacaatcag gtacctatgc gccggatgc
//
LOCUS       DAOQXS010000292         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24257_length_3861_cov_3.720704, whole genome shotgun sequence.
ACCESSION   DAOQXS010000292 DAOQXS010000000
VERSION     DAOQXS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24257_length_3861_cov_3.720704"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKM34_08475"
     CDS             complement(<1..1178)
                     /locus_tag="PKM34_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83663.1"
                     /translation="MLLTKLHIPSPGDQTVHREDLFDKLNCGLNRKLILISAPAGFGK
                     TTLVCDWIHQQKIPAAWFSIDKGDNDPEEFLSYIISGIQGVRKEFGQSALRLLQSPNK
                     PSPEAIATLLINDILRIKHDFILVLDDFHHVSSREVVELINFILQYIPGNIHLVILTR
                     SDPALPLARIRSQHQMVELRLADLSFTAHDISALFNKKLKLGLSNEDVYLLESRTEGW
                     IAGLQLAALSVQQRRDVSEFIRDLQGENQYIMDYLMEEVLKTQPDDIREFLLKTSLLD
                     QMNASLCDWVLNRNDSRFIIEHLERNNMFVIPLDSERNWYRYHHLFSDLLKQRFQQQM
                     ITNAADIRTKACDWFEQHQMTDLAIENALAGQNYEKSLQLISKVVEEISIPNPAPLIW
                     S"
     gene            complement(1214..1897)
                     /locus_tag="PKM34_08480"
     CDS             complement(1214..1897)
                     /locus_tag="PKM34_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ83664.1"
                     /translation="MAVNCIVIDDTPLAVEKLEGFIRQVPLLNLLASFNNALDAISFI
                     KGNPVDLVFLDIQMEQFSGLQFLESLRSPPQIIIVSAYDQYAVSGFDYSVTDYLLKPY
                     SFERFLKAIDKIKVDPAHKPSKDYMFVKTEYRMERINFADILYIEGQGAYLRIVTPKN
                     RIMTLQNFQTMESQLPADNFIRVHKSFIVAINKIESIERAIIKIGAQRIPIGAGYREK
                     FKAALNLKD"
     gene            complement(1887..2801)
                     /locus_tag="PKM34_08485"
     CDS             complement(1887..2801)
                     /locus_tag="PKM34_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ83665.1"
                     /translation="MGAYFLSGSNLFGIIFNFYLYILLPVGIFYACYFLLKPHNLKGQ
                     IFKLLTIIILPFIITTIFKYLTIRVGFEFPYFLVMTGLFAIPFATFGLLFRKMEVWIT
                     HERRTQENLKSELALLKNQISPHFLFNTLNNIDSLIKTNADQASETLIKLSGIMRYMI
                     YDTNAGRVPLSLEIKHIEDYIDLQKIQFSNRELVSFNVSGDTDRIQIAPMLFIPFVEN
                     AFKHCTDKNTRNAIRFSFRIEGNTVIFESSNIFNEEQKISRDHAGGIGLKNIKRRLEL
                     LYPDSHFLNFIQENSTFKVNLTVKINGR"
     gene            complement(3113..>3861)
                     /locus_tag="PKM34_08490"
     CDS             complement(3113..>3861)
                     /locus_tag="PKM34_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ83666.1"
                     /translation="SSLNSGSNGNCYYVGNDHDAVLIDAGISCRETEQRLKRQGLSLT
                     KVRAIFISHEHSDHTRGVEVLSRKHRIPVCITPATHRNSLIRLEPQLLQPFSAGEELH
                     FGSLTVKAFQKHHDAADPHSFTVTGSGITVGIFTDIGSVCEQLIHHFSRCHAAFLEAN
                     YDETMLEEGRYPYFLKRRIRGGNGHLSNLQALELFNSYRAPFMQLLVLSHLSRQNNHP
                     QIVTDLFAPHANGTRVTIASRFGESEVFAV"
BASE COUNT         1070 a          838 c          926 g         1027 t
ORIGIN      
        1 gaccaaatga gaggagctgg attgggaatg gaaatttcct caaccacctt gctgatcagt
       61 tgaaggctct tttcataatt ctggccggcc agtgcatttt caatggccaa atcggtcatt
      121 tggtgctgtt caaaccaatc gcaagctttg gtgcgaatat ccgcagcatt cgtaatcatc
      181 tgctgctgaa atcgttgctt taacaaatcg gaaaacaaat gatgataccg gtaccagttt
      241 ctttccgaat ccaaaggaat aacaaacatg ttattccttt ccaggtgttc aatgatgaaa
      301 cggctgtcgt tcctgttcag aacccaatca cataacgaag cgttcatctg atccagcagc
      361 gatgttttca acaagaattc cctgatgtcg tccggttgtg ttttcagtac ctcctccatg
      421 agataatcca tgatgtactg gttctcgccc tgcaaatccc ggatgaattc cgacacatct
      481 ctgcgttgct gcaccgataa agccgctagc tgtaacccgg caatccagcc ttctgttctg
      541 gattccagca aataaacatc ttcattggat aatcccagtt tcagcttttt attgaacagg
      601 gcggaaatat cgtgtgccgt aaaactcaga tcggccaaac gtagttcgac catctgatgc
      661 tggcttctga tccttgccag aggtaaagcg gggtccgacc gggtcaggat cacaagatga
      721 atgtttcctg gaatatattg caggataaag ttgattaatt caacgacttc gcggctgctg
      781 acatggtgga aatcgtcgag gaccagtata aagtcatgtt tgatgcggag aatatcgttg
      841 atcagaaggg tggcaatggc ttcgggagat ggcttatttg gcgactgcaa caacctaagg
      901 gcggactgtc cgaattcttt ccggacacct tgtattcctg aaataatata gcttaagaac
      961 tcttccggat cattgtcgcc tttatcgatg gaaaaccatg cggcagggat cttttgctga
     1021 tggatccagt cgcataccag ggttgttttg ccaaaaccgg caggtgcgga gatcaggatc
     1081 agcttacggt tcagcccgca attaagcttg tcaaacagat cttcccggtg aaccgtttga
     1141 tcacccggag aggggatatg tagttttgtc agaagcatag gctcattaat ctttccaggg
     1201 tgatggccca tcgtcagtct ttcaggttca gggcggcttt aaatttctca cggtagcccg
     1261 caccgatggg aatacgctgg gcgcctattt taatgatcgc ccgctcgatg ctttcaattt
     1321 tattgatggc tacaataaag gatttgtgga cccgaataaa attatcggcc ggcaactggc
     1381 tttccatcgt ttgaaaattc tgaagggtca taatcctgtt tttgggcgtt acgatcctca
     1441 gatatgctcc ctggccttca atatacagga tatcagcaaa attaatgcgt tccatccggt
     1501 attccgtctt cacaaacata taatctttag aaggtttatg agccggatca accttaattt
     1561 tatcgatcgc tttcaggaac cgctcaaacg aatagggctt cagtaaataa tcggtgaccg
     1621 aataatcaaa tccgctgacc gcatattggt catacgccga tacaatgatg atctgaggcg
     1681 gactccggag agactcaaga aattgtaaac ccgaaaattg ttccatctga atgtcaagaa
     1741 aaaccaggtc aaccgggttg ccttttataa atgaaatggc atccagtgcg ttgttgaacg
     1801 atgccaataa attcaacagc gggacctggc ggataaaccc ctccagcttt tcaacggcca
     1861 gaggggtatc gtcgatgaca atgcaattaa cggccattaa tttttacggt taaattcacc
     1921 ttaaatgtac tgttttcctg aatgaaattt aaaaaatgcg agtcgggata aagcagttcg
     1981 agcctgcgtt ttatattttt caggccgatg ccgcccgcat gatccctgga tattttctgt
     2041 tcctcgttga aaatattgct gctctcaaaa attacggtgt taccttcaat ccggaacgag
     2101 aaacgaatgg cgttccgggt gtttttatcg gtacaatgtt taaaggcatt ctccacaaaa
     2161 gggataaaaa gcatgggcgc aatttgaatg cggtcagtat ctccggaaac gttgaaagat
     2221 accagttccc tgtttgagaa ttgtattttc tgcaaatcga tatagtcttc aatatgcttg
     2281 atttcaagag ataacggcac cctgccggca ttcgtgtcat aaatcatata ccgcatgatg
     2341 ccggagagtt tgatcaatgt ttcggatgcc tgatcggcat tggtttttat caggctgtcg
     2401 atattgttca gcgtattgaa gagaaaatgc ggactgatct ggtttttaag cagcgccagt
     2461 tcactcttaa ggttttcctg cgttcgcctt tcatgggtga tccagacttc catctttctg
     2521 aaaaggaggc caaacgtggc aaaggggatg gcaaaaaggc cggtcatgac caggaaatac
     2581 gggaactcaa aaccaacccg gatggtcaga tatttaaaga ttgttgtaat aataaatgga
     2641 aggataataa tcgtcagtaa tttaaagatc tgccctttta aattgtgggg ttttagcagg
     2701 aaatagcagg cgtaaaatat tcccacgggg aggaggatgt aaaggtagaa gttgaaaatg
     2761 atcccgaaca ggttggaacc tgaaagaaag taggcaccca tatccggggg ctcggttaaa
     2821 aaacggaagc tgtctgataa taaagaccag aagcttactt gatctgttcc catcatatag
     2881 ctgaaggtgc caaattcacg ggacccaacg gagataaccc taaaaatcgg gaaaatcatc
     2941 cagaaaccgg catgcaataa aattttcact gaattcttca tggttgaaaa tgattaaagt
     3001 taaacatgaa gcaaaattca tcggaaatat aaggaataaa aaatccgttt aacaaacgcg
     3061 gcctgtttat tcatgaaatg acgggattta tgattaaggg ccggaagcgc cattacacag
     3121 caaaaacttc tgattcgccg aaacgggaag caatggttac gcgggtgccg ttggcatgag
     3181 gagcaaataa atccgttacg atctgcggat ggttattttg ccgggagagg tgtgaaagca
     3241 ccagaagctg catgaacggg gcgcgataac tgttaaagag ctccagcgcc tgcaggtttg
     3301 aaaggtgccc attgcccccg cggattcgtc ttttcaaaaa gtacggatag cggccttctt
     3361 cgagcatcgt ctcatcgtaa ttcgcttcaa ggaaagctgc gtgacaccgg ctgaagtgat
     3421 ggatcagctg ttcgcatacc gaaccgatat cggtaaaaat gcccactgtg ataccgcttc
     3481 ctgtcacggt gaagctatgc ggatcggctg catcgtgatg tttttgaaag gctttaacgg
     3541 taaggctgcc gaaatgcaat tcctcaccgg cggaaaacgg ctgcaaaagt tgcggttcaa
     3601 gccggatcag gctgttccgg tgagtggcag gagtgataca gaccgggatc ctgtgcttcc
     3661 gggaaagcac ctccacgccc ctggtatggt cagaatgttc atgggagata aaaatggccc
     3721 tgacttttgt caaggacagg ccctgtcgtt tcagccgttg ttctgtctcc ctgcacgaga
     3781 tgcccgcgtc gataaggacg gcatcatggt cgttgccaac atagtagcaa tttccattgc
     3841 ttccggaatt gagcgatgag a
//
LOCUS       DAOQXS010000293         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24319_length_3854_cov_3.994999, whole genome shotgun sequence.
ACCESSION   DAOQXS010000293 DAOQXS010000000
VERSION     DAOQXS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24319_length_3854_cov_3.994999"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..440
                     /locus_tag="PKM34_08495"
     CDS             165..440
                     /locus_tag="PKM34_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNQ83667.1"
                     /translation="MKKNIHPKDYRFVIFKDMSNNYSFLTKSTAKSKETATWEDGKEY
                     PLIKLEISNTSHPYFTGKMKLVDTAGRVDKFKNRYKQHLEKRESASE"
     gene            522..1703
                     /locus_tag="PKM34_08500"
     CDS             522..1703
                     /locus_tag="PKM34_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HNQ83668.1"
                     /translation="MNFILFDNFRRSNLFPLTYLRPVADIRTGILTIREKWERYLGQK
                     TSTLTESYLRKKFPVVREEDNIMINGSVCPNKQLLEEILCLKTGEVLFHGDAIIAMRL
                     HAVELEKLDDEHIDDVNMVESKSDILKITHTWDIFSQNDRAIREDFNLLTHGRVSGPV
                     SKSNQVIGNEVFIEKNAKVECCILNSSTGPIYIGEDAEIMEGSLIRGPFGLGAHATVK
                     MGAKIYGPTTIGPHCKVGGEINNSVFFGFSNKAHDGFMGQSVIGEWCNIGADTNTSNL
                     KNTYETIKLWNYADQTFVETGLQFCGLIMGDHSKCGINTMFNTGTVVGINSNIFGSGY
                     QRNFIPSFSWGGPSGIKSYHIDRAVSVARKVYQRRGLEFDQTEEDILRDVYNLTKEPL
                     P"
     gene            1853..>3854
                     /gene="lon"
                     /locus_tag="PKM34_08505"
     CDS             1853..>3854
                     /gene="lon"
                     /locus_tag="PKM34_08505"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNQ83669.1"
                     /translation="MDDLFNINGLSDAVDNDTEFIPLLSSEEEELMHSEKLPESLPIL
                     PLRNTVLFPGVVIPITVGRDKSIRLIRESYRNDRTIGVVAQKDSAIEDPGEKDLYNVG
                     TVAHLIKILQMPDGNTTVIIQGKKRFEIEGFIQNEPYFRAKIKDLTDFDKNLEQDEHF
                     LALIGSLKDLAIQIIKLSPNIPSEAAFAIKNIDSPFFLINFVSSNLNIDLVDKQKLLE
                     TPDLTKRANEVLTAMARELHMLELKNQIQDKVKLDLDKQQRDYLLNQQLKTIQEELGG
                     SPHEQEINDLKAKAAKKKWTKKIAEIFDKELKKLQRMNPQAAEYSIQMNYLETVVELP
                     WNEFTNDDFNLKKAQNILDEDHYGLEKIKERIIEHLAVLKLKNDMKSPILCFVGPPGV
                     GKTSLGKSIARALGRNYIRVSLGGLRDEAELRGHRKTYIGAMPGRIIQSLRKAGSANP
                     VFVLDEVDKVSGNNFQGDPQAALLEILDPEQNFEFYDNYLEINFDLSRVMFIATANNM
                     STIHPALRDRMEVIDLSGYLLEEKVEIARKHLIPKQIREHGLKEKHVAFPKKTIEKII
                     DDYTRESGVRSLEKVIAKIVRNRARLVAMEEGYTPKLSNQDIETIMGAPRYQKDRDSR
                     IDVPGVVTGLAWTAAGGEILFIEVSRSRGKGNLTLTGNLGDVM"
BASE COUNT         1183 a          847 c          915 g          909 t
ORIGIN      
        1 cctttcggaa aaagccagat gcctgaatta tgggattatg ccgatggcat cgatacaatg
       61 gaattcctgt ttagcttata atttttaagt tttttcggta tcggatttgg ttattacaat
      121 aaaataacta cctttgcagc ccgaatttga aatggaataa gatcatgaag aaaaacattc
      181 acccgaaaga ttaccgtttt gtcattttca aggacatgtc gaataattat tcattcctta
      241 ccaagtcgac ggctaagtca aaagaaacgg ctacatggga agatggaaaa gaatatccgc
      301 tgatcaaact tgaaatatcg aatacatccc atccctattt caccggtaag atgaaactgg
      361 ttgatacggc cgggcgtgtt gataaattca agaacaggta caaacagcac ctggaaaaga
      421 gagaaagcgc atctgaatag acactttttt cacactagta taaaagccct ttgcctgcgc
      481 tgagggcttt ttttgtttta tttttgaaat aaaaagaaga tatgaatttt attttatttg
      541 ataattttcg cagaagcaac cttttccccc tgacatatct ccgtccggta gctgatatcc
      601 gcacagggat tctgaccatc cgggaaaaat gggaacggta cctgggccag aaaacctcca
      661 ctttaacaga aagctatctc cgtaagaaat tccctgttgt cagggaagag gacaacatca
      721 tgatcaacgg gtcagtctgc ccgaataagc aactgctgga ggagatcctc tgcctgaaaa
      781 ctggcgaggt actgttccat ggagatgcaa tcattgccat gaggttgcat gccgttgaac
      841 ttgagaagct tgatgatgag catattgacg atgttaatat ggtcgaatcc aaaagcgaca
      901 ttttgaaaat tacccatacg tgggatatat tttcacaaaa tgacagggca atacgtgagg
      961 atttcaattt actcacgcac ggcagggttt caggccctgt cagcaaatcc aatcaggtaa
     1021 ttggaaatga ggtatttata gagaagaatg caaaggtgga atgctgcata ctgaattcat
     1081 caaccggtcc tatatacatc ggtgaagatg cggaaatcat ggaaggcagc ctgatcagag
     1141 gccctttcgg cctgggggcg catgctaccg ttaaaatggg tgcgaaaata tacggcccta
     1201 ctacgatagg acctcactgc aaagtgggag gggagatcaa caacagcgtg tttttcggat
     1261 tctccaacaa ggctcatgat ggtttcatgg gacaatcggt gatcggcgaa tggtgcaata
     1321 ttggcgctga caccaataca tccaacctta aaaatactta cgaaacaatt aagctgtgga
     1381 attatgccga ccagactttt gttgaaaccg ggctgcagtt ctgcggcctg atcatgggcg
     1441 accactccaa atgcggcatt aatacaatgt ttaataccgg aacggttgtc ggcatcaatt
     1501 ccaacatatt cggatcgggt taccagagga actttatacc cagtttttcc tggggagggc
     1561 cttccggaat caaatcctac cacatcgacc gggccgtgtc cgtggccagg aaagtatacc
     1621 agcggcgcgg cctcgaattc gatcagacag aagaagatat cctgcgcgat gtttataatt
     1681 taaccaagga accgttgcca tagcctcacc gaacctttga ttctggcata gtagttgaaa
     1741 tacggcaggg caatttcccg gcaaccatca ggttatatcc atgtttgaag cagagaaagg
     1801 gcacaccgga tttgcatacc gaaaatacta ctgacaattt gacactcgtt ttatggatga
     1861 tctctttaat ataaatggcc tttccgacgc ggttgataac gatacggaat tcattcctct
     1921 tttatcgtca gaagaagaag agttaatgca ttcggaaaag ctgcccgaat ccctcccgat
     1981 tctgcccctt cgaaatacag tcctttttcc cggtgtggtt attcccatta cagtcgggcg
     2041 tgataaatcc atccgcctga tccgggaatc ctaccgtaat gaccggacca tcggcgtggt
     2101 ggcacaaaag gattcggcca tagaggaccc gggagaaaaa gacttgtata atgtgggcac
     2161 cgtcgcccat cttattaaaa tcctgcagat gcccgacgga aacacaacag tcatcattca
     2221 aggaaaaaaa agatttgaaa ttgaaggttt tattcaaaac gaaccctatt tcagagcaaa
     2281 aatcaaggac ctgaccgact tcgataaaaa tctggaacag gatgagcact ttctggccct
     2341 gatcggatcc ctgaaagacc tcgctatcca gataatcaag ctttcgccga acataccttc
     2401 cgaagccgca ttcgccatta aaaatatcga cagccctttt ttcctgatca attttgtatc
     2461 ctccaacctg aacatcgacc tggtggataa acaaaaactt ctggaaacgc ccgatcttac
     2521 caagcgggcg aatgaggtcc tgacagccat ggccagggag ctgcatatgc ttgagttgaa
     2581 aaaccagatc caggataaag taaagctcga tcttgacaaa caacaacgcg attacctgct
     2641 gaaccagcaa ttaaaaacaa tccaggaaga gcttggcggt agtccgcatg aacaggaaat
     2701 caatgacctg aaagccaaag cggcgaaaaa gaaatggaca aagaagattg cggagatttt
     2761 tgacaaagag ttgaaaaagt tgcaacggat gaatccgcag gcggcggagt attcgatcca
     2821 gatgaattac ctggaaaccg ttgtcgagct gccctggaac gaattcacaa acgatgattt
     2881 caacctgaaa aaggcccaga acattttgga tgaagaccat tacggtctgg aaaagataaa
     2941 agaaaggatc atagaacatc tggcggtttt aaagctgaaa aatgacatga aatcgcccat
     3001 tttatgtttt gtcggccccc cgggtgtcgg taaaacatcc ctgggaaaat cgattgcccg
     3061 ggcattggga cgaaactaca ttcgggtctc tttagggggg ctcagggacg aggccgaact
     3121 cagagggcac aggaaaacat atatcggcgc catgccgggc aggatcattc aaagccttag
     3181 aaaagcaggg tcggcgaacc cggtatttgt ccttgatgag gtggataaag tcagtgggaa
     3241 caatttccag ggagatccac aggcagcctt gcttgagata ctggacccgg aacagaactt
     3301 cgaattctat gataactacc tggagatcaa cttcgatctc tcgagagtga tgttcattgc
     3361 caccgctaat aatatgtcga ctattcatcc ggcactccgc gacaggatgg aagtaatcga
     3421 cctcagcggc tatcttctgg aagaaaaagt tgaaattgcc cgcaaacacc tgatcccaaa
     3481 acaaatcagg gaacacggat tgaaagaaaa gcatgtggca ttcccgaaaa agaccattga
     3541 aaagatcatc gatgattata caagggaatc gggcgtccgg tccttggaga aggtgattgc
     3601 gaaaatagtc cgtaacaggg cccgccttgt ggccatggaa gaaggttaca ctccaaaatt
     3661 atccaaccag gatattgaga cgatcatggg agcgcccagg taccagaaag atcgcgattc
     3721 ccggatagat gtgcccgggg tggtgacagg cctggcgtgg actgccgcag gaggggagat
     3781 actatttata gaagtcagcc gcagcagagg gaagggaaac cttaccctta caggaaacct
     3841 gggggatgtc atga
//
LOCUS       DAOQXS010000294         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24346_length_3851_cov_3.558219, whole genome shotgun sequence.
ACCESSION   DAOQXS010000294 DAOQXS010000000
VERSION     DAOQXS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24346_length_3851_cov_3.558219"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..925)
                     /locus_tag="PKM34_08510"
     CDS             complement(167..925)
                     /locus_tag="PKM34_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83670.1"
                     /translation="MIKTILITGATAGIGKATAFRFAQEGHRLIITGRRIKILQDIAT
                     GLKEKYNTETVCLAFDIRDRSAMEKSLDSLPEEWKAIDILVNNAGLAAGLDPVNTAST
                     DDWDTMIDTNVKGLLYISRKVIPWMIERGKGHIFNIGSIAGKEVYPKGSVYCATKHAV
                     DALTKGMRIDLLPHGIKVTQVSPGAVETEFSLVRFKGDHDRAGMVYKGYEPLVAEDIA
                     AVIHFISSLPPRVNINDILVMPQAQANATTFNKM"
     gene            complement(931..1797)
                     /locus_tag="PKM34_08515"
     CDS             complement(931..1797)
                     /locus_tag="PKM34_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ83671.1"
                     /translation="MKTEYILTRRKEKLSSLTRKKLLLAMIISPLLAFTQGGYRITPD
                     VVYGHKAGMALTYDVFQPAGPANGAGLIHLVSGGWNSRYNPPDSVIEDYKPYLDSGFT
                     VFALRHGSSPTFKLPETIDDVILGSWQIRRNAYRFGVDSLRLGIFGGSSGGHLSLMAG
                     LSGDRHPVSAIVAFFPPVDLRNIPDFMKTMFPAFDFDTTLAAKISPVLFASPDDPPTL
                     LIHGDKDFVVAPRQSERMIAALEENSVVSQLIVFEGMGHGNSYGAKGKYYEESTRETI
                     GWFRKYLVDPYK"
     gene            complement(1836..2726)
                     /locus_tag="PKM34_08520"
     CDS             complement(1836..2726)
                     /locus_tag="PKM34_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNQ83672.1"
                     /translation="MKQKNHTLSRFQSIVLSLLIYIIAGITAVLIARLFTGHHPLVMI
                     GAGDLAATVVVFLFSVILNNSSAYDPYWSVKPIVIAAAYVFIFHLDNLTIRQLLVLAG
                     IFLYALRLTSNFYRDWPGFHHEDWRYVNFRKSSGKAYWVVSFLAIHLFPTIMVYLGCM
                     AMFAVFSGEGRALNIWDIPAALILFGSVVYVFVADEQLRNFRKDSSNKGKTIMSGLWR
                     LSRHPNYLGEISTWWGLALFAIAAGCHFWWTLAGPVAITLMFIFASIPMIEKRNLQRR
                     EGYTEYRKNTPMLLPLKFFR"
     gene            complement(2775..3698)
                     /locus_tag="PKM34_08525"
     CDS             complement(2775..3698)
                     /locus_tag="PKM34_08525"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNQ83673.1"
                     /translation="MIRQAVIVLVSIIPTIIFSQNNMKYNKLTTEEEYIIIQKGTERP
                     WTGKYLNNKETGTYTCRRCDAPLYRSEDKFDSHCGWPSFDDEIKGAVKRIPDPDGMRT
                     EIICASCGGHLGHVFLDEGFTDKNTRHCVNSISMNFIPDSEAANQAVNRAIFAGGCFW
                     GVEYYMQKKPGVISVVSGYIGGHKDNPTYQEVCTGTTGHAEAVEITFDPSKVSYEELA
                     RLFFEIHDPTQVNRQGPDVGAQYRSEIFYLDENQKIITENLIGILKNKGFKVATKVTK
                     ATTFWPAENYHQDYYDNKGTLPYCHGYTKRF"
BASE COUNT          976 a          910 c          950 g         1015 t
ORIGIN      
        1 aaaaaggaag aagatcaaag agtctggtta cttccatgtg gtttgatttg ggatctaaaa
       61 agcgcgcaaa gatataaaaa tatttcctat atagtatttt atatatgagc tacttaaaaa
      121 gaggcctaaa agtaataaat cttgaaatga aaaaatcaag aataaatcac attttattaa
      181 aagttgttgc attcgcctga gcttggggca ttacaaggat atcattgata ttgaccctcg
      241 gcggaagcga ggagataaaa tgtatgaccg cagcaatatc ttctgccacc agcggttcat
      301 agcctttata aaccatcccg gcccggtcgt gatcgccttt gaaacgcacc agggagaatt
      361 ccgtttccac agcgccgggg ctgacttggg tcactttaat gccatggggg agcaaatcga
      421 tccgcatccc tttggtgagg gcatccacgg catgcttggt ggcgcaatag acgctacctt
      481 tcgggtacac ttcttttccg gcgatggatc caatgttgaa aatatgcccc ttgccccttt
      541 cgatcatcca ggggatcact ttacgggaaa tatacagcaa acccttcaca ttggtatcga
      601 tcatggtatc ccagtcgtcg gtcgaagccg tgttcaccgg atccagcccg gcagccaggc
      661 cggcattatt gaccaggata tcgatcgctt tccattcttc ggggaggcta tcgagactct
      721 tctccatagc tgatctatcg cggatatcaa atgccaggca aacagtttcg gtgttgtatt
      781 tttccttgag cccggtggca atatcctgca ggatttttat tctccggccc gtgatgatca
      841 ggcgatggcc ctcctgggca aaccggaatg cagttgcttt tcctatccca gccgttgcgc
      901 cggtaatgag tatggttttt atcatgaggt ttatttatat ggatctacga gatatttccg
      961 gaaccagccg atggtttccc tggtgctttc ttcataatat tttcctttgg cgccgtagct
     1021 gttgccgtgt cccatgcctt cgaagacgat cagttgcgaa accacgctat tttcctccag
     1081 cgcagcgatc atccgttccg attgcctggg cgcaacgaca aaatctttat caccgtgtat
     1141 cagcaatgtc ggagggtcat cagggctggc gaaaagcacg ggagatattt tggcggccag
     1201 ggtggtatca aagtcgaatg cggggaacat ggttttcatg aagtcgggga tattcctcag
     1261 gtcgaccggc gggaagaaag cgacaatagc gctgaccggg tgcctgtcgc ccgaaaggcc
     1321 ggccattagt gaaagatggc cgccggagct gcctccgaaa atgcccagcc tcagtgaatc
     1381 gacgccaaaa cggtacgcat tcctgcggat ttgccatgat cccaggatga cgtcgtcgat
     1441 ggtctcagga agcttgaaag tcgggctgct gccatggcgc aaggcaaaaa cggtaaatcc
     1501 cgagtcgagg taaggcttgt aatcttcaat gactgagtcg ggcgggttat agcgggaatt
     1561 ccatcccccg ctgaccaggt ggatgaggcc tgctccgttg gccggtccgg ccggctggaa
     1621 gacatcgtag gttaacgcca ttcccgcctt atggccataa accacatcgg gtgtaatacg
     1681 gtatccaccc tgggtaaagg ccagaagcgg gctaataatc atggcaagaa gtaacttctt
     1741 acgggtcagg gagctgagct tttccttccg acgggttaat atatattcag ttttcatggc
     1801 ggtataacaa acagaaatgg atgtttgtta aaggattatc tgaaaaattt caatggcagc
     1861 agcatcggtg tgttcttccg gtattccgtg tatccttccc tgcgctgaag gttgcgtttt
     1921 tcaatcatcg ggatgctggc gaaaatgaac atcagggtta tggcgaccgg gccggccagt
     1981 gtccaccaga aatggcagcc tgcggcaatg gcaaataaag ccagccccca ccaggtactg
     2041 atctctccca ggtaattggg atggcggctt aagcgccaca ggccggacat gatcgttttt
     2101 cccttgtttg agctgtcttt tctgaagttc ctgagctgtt catccgcaac aaacacataa
     2161 accacgcttc caaaaaggat cagggcggcc gggatatccc agatattcag tgcccttcct
     2221 tcaccggaaa atacggcaaa catagccatg cacccaaggt agaccataat ggtcgggaac
     2281 aggtggattg ccaggaaact caccacccag tatgcttttc cgctggattt cctgaaattt
     2341 acatagcgcc agtcttcatg atggaacccg ggccagtcgc ggtaaaaatt cgaagtaaga
     2401 cgaagggcat acagaaaaat accggcaaga accagcaact gcctgatggt caggttatca
     2461 agatgaaaaa taaaaacata agctgccgcg atcacaatag gttttacact ccaataggga
     2521 tcataagcgg aggaattgtt caggatgacc gaaaacagaa agacgaccac ggtggctgcc
     2581 aggtctcctg ccccaatcat gaccaaggga tgatgaccgg tgaataacct tgcaataagc
     2641 actgcggtta tgccggcgat aatgtagatc aacaggctga gcacaatgct ctgaaacctg
     2701 cttagagtat gatttttctg tttcattttt aattcatttg ccattcaggc acctaaactc
     2761 caattcatta aaaactagaa tctcttcgta tatccatggc aatagggcaa ggtgccttta
     2821 ttgtcgtaat aatcctggtg gtaattttct gccggccaga aggttgtcgc ttttgtgact
     2881 ttcgtggcca ctttgaagcc tttgtttttg aggattccga taaggttttc agtgattatc
     2941 ttctgatttt catccaggta aaaaatttcg gaacggtact gtgcgccgac atcgggaccc
     3001 tggcggttga cctgggtcgg gtcgtggatt tcgaagaaga ggcgggccag ttcttcgtaa
     3061 gacactttag aaggatcgaa ggtaatctct actgcttcgg catggccggt ggttcccgtg
     3121 cagacctcct ggtaggtagg attatcttta tgtccgccga tataacccga aacgacagag
     3181 atcacacccg gtttcttctg catatagtat tcgacgcccc aaaagcatcc tccggcgaat
     3241 attgcccggt ttacggcctg attggccgct tctgaatcag gaataaaatt catagagatg
     3301 gaatttacgc aatgacgtgt gtttttatct gtaaagcctt catccagaaa gacgtgcccc
     3361 aggtgtccgc cgcaactggc gcagatgatc tctgtccgca taccatcggg atcgggaatc
     3421 cttttgactg cccctttgat ctcatcgtcg aagctgggcc atccgcagtg ggagtcgaac
     3481 ttatcttccg acctgtaaag cggggcgtca caccggcggc aggtataagt acctgtttct
     3541 ttattgttca ggtattttcc agtccacggg cgctcagtcc ctttttggat gatgatatat
     3601 tcttcctccg tggttaactt attgtatttc atattgtttt gcgagaatat aatggttggg
     3661 atgatggaga ctaaaacgat tacagcttgc cggatcatta tcatcaattt taacacctaa
     3721 ccgttttgtg ccaattttgt ttaaaaattt gttgatcccc tccggggaaa aattacaaca
     3781 cgctcatttt tctataagaa ttgatcccct acggggaata taatacatcg atgatcgcct
     3841 gttcgcataa t
//
LOCUS       DAOQXS010000295         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24373_length_3849_cov_3.943595, whole genome shotgun sequence.
ACCESSION   DAOQXS010000295 DAOQXS010000000
VERSION     DAOQXS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24373_length_3849_cov_3.943595"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1184
                     /locus_tag="PKM34_08530"
     CDS             180..1184
                     /locus_tag="PKM34_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83674.1"
                     /translation="MTDRKKDHIDLAFKSKTGTDEIDRRFYYEPLLSHHPVNDLRPFR
                     FLGHTLRAPLWVSSMTGGTELAGPINRNLARVCREFGLGMGLGSCRPLLKSDEHFADF
                     DMREIIGKDLPFYANLGIAQLESFVLNGDITPVDRLVDKLKADGLIIHVNPMQEWLQP
                     EGDLFSQPPIDTIRSFLDMAAYPVIVKEVGQGMGPESLRALLQLPLEAIELAAFGGTN
                     FAKVELLRSDETRRQYYEPLSHIGHDAFEMTEMINTIVEEKISINCRQIIISGGIQTF
                     LDGYCLISRCKLPAIYGQASGFLKYASKSYDDLYTFVKYQVDGIKLANAYFVTKDLKK
                     "
     gene            1181..2524
                     /locus_tag="PKM34_08535"
     CDS             1181..2524
                     /locus_tag="PKM34_08535"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HNQ83675.1"
                     /translation="MNINGIINGFSKMSKEEKLKLVAGLFDHPEKVLKELKSYWHRDP
                     KKQTLFDEFSENTLSNFYFPFGIAPNFLINNKNYMVPMVIEESSVIAAASSSAKFWAE
                     RGGFHAEVVSSEKLGQVHFVWKGDPEKLKQAMPALKQELLAGTKAITANMESRGGGIL
                     DVELINMNDRIEDYYQLKVSFETVDSMGANFINSVLEEFARILKEFLVKSPVFNEDEK
                     DCEIIMSILSNYTPDCLVRAWVECDISRMHLGENDMNEEKFVWKFQKAIEIAKVDEYR
                     ATTHNKGIFNGIDALALATGNDFRAIEAGAHTYACRTGKYQSLTELTLDNGMFHYELK
                     VPLAMGTVGGLTSLHPMARFSLEMLGKPSARELMIIAACAGLANNFGAVKSLVTKGIQ
                     VGHMKMHLFNILNFYAATQEEKLKAVEYFKNKKVSFKSVTDFINNHRSQILGVKS"
     gene            2528..3448
                     /locus_tag="PKM34_08540"
     CDS             2528..3448
                     /locus_tag="PKM34_08540"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNQ83676.1"
                     /translation="MQTGQFYSNGKFLISGEYAVIHGAKALAVPLRFGQEMSVTEMDS
                     GGLIHWQTKVQGIDWFSAIFNRKRFEVTETSDPTTAGFIRQLLLEASSFQPELARGTS
                     SFFIENEIGFDINWGLGSSSSLISNISYWLDIDPYALYKELFRGSGYDLFCARTDHSI
                     VFQLKNGEPVVQTDVFNPPFQHHIHFVYSGRKQDSQSSVSNFLKEGRIDPGILHKISH
                     ITDRMTMASDVAEFMELMNEHETIISDLIGLPPVQRELFPGFDGAIKSLGAWGGDFIM
                     AVSPRDDKYVKDYFFSKNLPVIFAWHEIVR"
     gene            3432..>3849
                     /locus_tag="PKM34_08545"
     CDS             3432..>3849
                     /locus_tag="PKM34_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83677.1"
                     /translation="MRSSDKTVGNIAWRSPSNIALVKYWGKRPVQIPANPSLSMTLGK
                     AYTEMNFSYRRADKGEKKLDFLFEGLKNEKFALKIATFLDSLSGNHPEISNFHIQVSS
                     FNTFPHSTGIASSASSMSALGLCLAELDPDAMPGELT"
BASE COUNT         1036 a          942 c          942 g          929 t
ORIGIN      
        1 aggcctcccg ggttccgctt gagaaatggc tcgataaaaa ggcgcccggt tacaatccga
       61 aatgggaaac cggcctcgga caggtcgacg cttacatccg caacgaactg atcaaatcgt
      121 taaaggagaa gatgaaacct cccaaagagg tgaaagaaga catttggtaa cccgatatca
      181 tgaccgaccg aaagaaagac catattgacc ttgcattcaa atcaaagaca ggaaccgatg
      241 aaatagaccg ccggttttac tatgaaccgc tgttgtcgca tcaccctgtc aatgacctgc
      301 gtcctttccg gttcctggga cataccctgc gtgcccccct ctgggtttcg agcatgaccg
      361 gaggtacgga actggccggc ccgatcaaca ggaacctggc cagggtgtgc agggagttcg
      421 gcctgggcat gggccttgga tcctgcaggc cgctgcttaa aagcgacgag cattttgccg
      481 atttcgacat gcgggaaata attggcaaag acctgccatt ttatgccaac ctgggtatag
      541 cccagctcga atccttcgtg ctgaacggcg acattacacc ggtcgaccgg ctggtggata
      601 aactgaaagc cgacgggctt atcatccatg tgaacccgat gcaggagtgg ctgcagccgg
      661 aaggagacct cttcagccag cccccgatcg atactatccg gtcatttctt gatatggcag
      721 cgtatccggt cattgtcaaa gaagtcgggc aaggcatggg gcccgaaagc ctgcgtgcac
      781 tgctgcaact gccgctcgaa gccatcgaac tggccgcttt cgggggaacc aacttcgcca
      841 aagttgaact gctcaggtcg gatgagaccc gccggcaata ttacgaacca ctcagccata
      901 tcggccatga tgcttttgaa atgaccgaaa tgatcaatac tattgtggag gaaaagatca
      961 gcatcaactg ccggcaaatc attatttccg gcggtataca gacgttcctg gatgggtatt
     1021 gcctgatcag ccgctgcaaa ctgccggcca tctacggcca ggcatccggt tttctgaagt
     1081 acgcttcaaa atcgtacgac gatctttaca cttttgtaaa ataccaggtt gatgggatca
     1141 aactggccaa tgcctatttt gtcaccaagg atctgaaaaa atgaacatta acggcattat
     1201 caacggtttc tccaagatga gcaaagagga gaagctcaaa ctcgttgcag gccttttcga
     1261 tcatcccgaa aaggtgttga aagagctaaa atcatactgg caccgggatc cgaaaaaaca
     1321 aaccctgttt gatgagttca gtgaaaatac cctgtcaaac ttttattttc ctttcggaat
     1381 agcaccaaat ttcctgatca acaacaagaa ctatatggtc ccgatggtca tcgaggaaag
     1441 ttcggtgatt gccgcagcat cgagcagcgc taaattctgg gccgaaaggg gtggattcca
     1501 tgcggaagtg gtctcttcgg aaaaactggg acaggttcac tttgtctgga aaggtgatcc
     1561 ggagaagctc aaacaggcga tgcccgccct gaaacaggaa cttctggccg ggaccaaagc
     1621 catcacggcc aatatggaga gcaggggagg aggaatcctc gatgttgagc ttatcaatat
     1681 gaatgaccgg atcgaagatt attaccagtt gaaagtttct ttcgagaccg tcgattcgat
     1741 gggggctaat ttcatcaact ccgtcctgga agaatttgcc cgtattctca aggaatttct
     1801 tgttaaaagc ccggttttta atgaagatga gaaggattgc gagatcatta tgtcaatcct
     1861 ctccaattat acacccgatt gcctggtcag agcctgggta gaatgcgata tcagccggat
     1921 gcacctgggt gaaaacgata tgaacgagga gaagttcgtt tggaagtttc agaaagccat
     1981 tgagattgcc aaagtcgatg aatacagggc cactacccac aataaaggga tattcaacgg
     2041 gatcgacgca ctggccctgg cgaccggtaa cgacttccgt gcgatcgaag ctggcgccca
     2101 tacctatgcc tgccggaccg gcaaatacca gagcctgact gagctgaccc tcgacaatgg
     2161 aatgtttcat tacgaactca aagtgcccct tgccatgggt accgtcggag ggctgacctc
     2221 cttacaccct atggcacgct tctctcttga gatgctaggc aaacccagcg cccgcgaact
     2281 gatgatcatt gcagcctgtg ccgggcttgc caataatttt ggcgccgtta agtcgcttgt
     2341 caccaaggga atacaggtcg ggcatatgaa aatgcacctg ttcaatatcc tgaatttcta
     2401 tgccgctaca caggaggaaa aactgaaagc tgttgaatac tttaaaaaca aaaaggtttc
     2461 tttcaagtcg gttaccgatt tcattaataa tcacaggtca cagatcctgg gtgtaaaaag
     2521 ctgagccttg cagacgggac aattttattc caatggtaaa ttcctgattt ccggggaata
     2581 tgctgttatt cacggcgcta aagcactcgc cgtcccgctc cgcttcggcc aggaaatgtc
     2641 tgttacagaa atggattccg gcgggttgat ccattggcaa acaaaagttc agggaattga
     2701 ttggttttct gcaattttca atagaaaaag atttgaggta accgaaacct ccgaccccac
     2761 cactgccggc tttatccggc aattgctcct ggaagcatct tcgtttcagc cggaactggc
     2821 ccgtggaacc tcctcctttt tcatagaaaa cgagattggt ttcgatatta actggggatt
     2881 aggaagcagt tcgtcgttaa tctccaatat ttcctactgg cttgatattg acccatatgc
     2941 cctgtataaa gagcttttca ggggctcggg atacgatctg ttctgcgccc ggacagatca
     3001 ttccatcgtt ttccagctga aaaacgggga acctgtggtt cagaccgatg tattcaatcc
     3061 cccttttcag catcatattc atttcgtcta ttcaggacgt aaacaggatt ctcagtccag
     3121 tgtcagtaat tttctgaaag aaggccggat tgatcccggt attctccata aaatcagtca
     3181 tataacagat aggatgacca tggcgtcgga tgtagctgaa ttcatggaat tgatgaatga
     3241 acatgagact atcatctccg atctgatcgg tctgccgccg gttcaaaggg aactattccc
     3301 cggtttcgat ggcgcaatca agtcactcgg ggcatggggc ggcgatttca tcatggctgt
     3361 atcgcccaga gacgacaagt acgtcaaaga ctatttcttc agtaaaaatc ttcctgttat
     3421 cttcgcatgg catgagatcg tcagataaaa ccgttggaaa catcgcctgg aggagcccgt
     3481 cgaatatcgc cctcgtgaaa tactggggta aaaggccggt tcagataccg gctaatccct
     3541 ccttaagcat gaccctgggt aaagcttata cggaaatgaa tttttcctac cggagagcgg
     3601 acaaaggtga aaaaaagctg gattttcttt ttgaggggtt gaagaatgaa aaatttgctt
     3661 taaagattgc aactttcctt gactcattaa gcggcaacca tccggaaata tcaaatttcc
     3721 acattcaagt ttcctctttc aacaccttcc cccattcgac cggaattgcc tcttcagcct
     3781 cgagcatgag cgccctcggc ctctgcctgg ctgaattaga cccggacgcg atgccagggg
     3841 agctgacgg
//
LOCUS       DAOQXS010000296         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24495_length_3835_cov_3.921958, whole genome shotgun sequence.
ACCESSION   DAOQXS010000296 DAOQXS010000000
VERSION     DAOQXS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24495_length_3835_cov_3.921958"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKM34_08550"
     CDS             complement(<1..803)
                     /locus_tag="PKM34_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83678.1"
                     /translation="MIRIRNLSDSAIRIKPVLMLPDGWLSFTTPVNDTLIGANDSISI
                     PIRFQLPEKVSSEISHDIAFRAYSMQNKLLSETHCTVKPEAFHKWDVTLPDNRVFFYP
                     RNNNSDFTIFIENKGNTDEQIDLNIITDRKVELVSTGAWQPGQPINIKPFQDTTLKLN
                     AKYINDDQRVFDLSKIQVIAEAGEAKFNQSFMIEKYNDTYAPLIFDRTLPHQVELGIR
                     TFTGNQEFLPFIKARGIATIKSTSSFTYNFNYYSVTGAENIISNSYYNFL"
     gene            complement(1022..1651)
                     /locus_tag="PKM34_08555"
     CDS             complement(1022..1651)
                     /locus_tag="PKM34_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83679.1"
                     /translation="MDKLFKIRLLTTLLTGFSAIYAFSQETPMLISTEAKLDNVLLLK
                     MTPEKYVEFTIKKINENLYQITDQPDDVMFSVESTGNWNLSISAEDPYFTGVSDSSHK
                     IPVDFIGLFVENIGSNWDNGLFSNMANATKDTVVSLSPDKTSLLEAGVRGNVGGSLQN
                     AFILRWKFFYEDAALKTRKFTDYIIEDDTYKVGYYITLSESTANSKIDQ"
     gene            complement(1756..2376)
                     /locus_tag="PKM34_08560"
     CDS             complement(1756..2376)
                     /locus_tag="PKM34_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83680.1"
                     /translation="MKRIILLLICFTAIQLNSVTAQLTDDEEVVFNAILGGAFNLVVQ
                     DGDVQTATFGAADEYNLGISETMGNPGIDPGFTTVAMEATGNWYLKISAPDFTPIVGT
                     GVIPINNLGVWCEATGVHQFGTEVACAYQSADAALGLSNTDITLIDLLTDNSGDQTDN
                     LFVLHWLFGTMQGTMNPASMFSQLSDGIFSQGTYTSTVVLTMTEIP"
     gene            complement(2526..3146)
                     /locus_tag="PKM34_08565"
     CDS             complement(2526..3146)
                     /locus_tag="PKM34_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83681.1"
                     /translation="MKKIVLLFGLLFALQAAIMAQLTDQEVVVFNAHLNETFNLNVDG
                     VTQEITFAVAADYNNGVTEAGGIVPGFTLISVEATGDWELEISAPDFTPGGGPNPGTG
                     AIPINNLGVWCQATGTHQFGTEVTCAYQNAAAAMGLTNADQLLIGLGTTNAGDISDNA
                     FTLHWLMGTMQGSMNTESMFNQLALGTLFSTGDFTTTATLTLTEIP"
     gene            complement(3374..>3835)
                     /locus_tag="PKM34_08570"
     CDS             complement(3374..>3835)
                     /locus_tag="PKM34_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83682.1"
                     /translation="TGYSIQFVFDEIDEYINGIQNAGQSTFIRIGAIYDWKLQFKADQ
                     DMFYGTFDPAHQMELNNVGVTVNSIGTNLDDGTHILNYAKAAPLALEYTDVTLLTKGT
                     LSNKGYGIENTFTLDWEMGTRNGNMNQTRMLDQLIPADSYTLNIILTLSVY"
BASE COUNT         1015 a          810 c          937 g         1073 t
ORIGIN      
        1 aggaaattat aataactgtt gctgatgatg ttctcagccc ctgttacgga atagtaattg
       61 aaattatagg taaatgagct ggtgctcttt attgtcgcta ttcctcttgc tttgatgaac
      121 ggaaggaatt cctggttgcc tgtgaatgtt ctgatcccga gctctacctg atgggggaga
      181 gtcctgtcga agatcagcgg tgcatacgtg tcgttgtatt tttcgatcat gaacgactgg
      241 ttgaatttag cctcgcccgc ttcggcgatt acctgtatct tgctcaggtc aaatacccgc
      301 tggtcatcat tgatatattt ggcgttcagc ttcagggtcg tgtcctggaa cggttttata
      361 tttataggct ggcctggttg ccatgctccg gtactgacca gttccacttt tcggtcggta
      421 atgatattaa gatcaatctg ctcgtctgta ttgcccttat tttcaatgaa aatggtaaaa
      481 tcagaattgt tgttacgcgg ataaaagaat acgcggttat ccggaagtgt aacgtcccat
      541 ttgtgaaaag cctccggttt gacagtacaa tgtgtttccg aaaggagttt gttttgcatg
      601 gagtaggccc ggaaagcgat gtcgtggctt atttcggaac tgactttttc gggaagttga
      661 aaacggatcg ggatggagat ggagtcgttg gctccgatca gcgtatcatt aacgggtgtg
      721 gtaaaggaga gccagccgtc ggggagcatg agtaccggtt taattcggat agcagaatcg
      781 gaaaggtttc tgattcggat cacattaaaa ctaagagcgc cacttttcag gccgacattg
      841 ggtttgatga atgaagcgga aaggatggcg ccagcctgtc ctccgtcagg ttgccgggct
      901 tgtgagatca ggcttttccc cgataagaac aggagccctg aaaaaatgag tatcttaaag
      961 tcaagtttca gtttttatag cggataaatt accggtctct gccggcccct atgcagggat
     1021 tttattgatc aatcttactg tttgcggtac tttccgataa agtgatataa taccccacct
     1081 tgtatgtgtc gtcttcaatg atgtaatcgg tgaatttcct ggtcttcagg gccgcgtctt
     1141 cataaaagaa cttccaccgg aggatgaatg cgttttgaag cgagccgccg acatttcccc
     1201 tgacgcctgc ctcgagcaaa ctggttttat cgggcgacag gctgaccacc gtgtctttgg
     1261 ttgcattagc catgttggag aaaagaccgt tatcccagtt agagcctatg ttttcaacaa
     1321 acagcccgat gaaatcgacg ggaatcttgt gtgaagaatc gctaactccg gtaaaataag
     1381 gatcctctgc agaaatggaa aggttccagt tgccggtcga ttcgacgctg aacatcacat
     1441 cgtccggctg atccgttatc tggtaaagat tttcattgat ctttttaatg gtaaattcga
     1501 catatttctc cggcgtcatc ttcaataaca acacattatc gagtttagcc tcggtactga
     1561 tcagcatcgg ggtctcctgg gagaacgcat aaatagcgga aaaccctgtc agcagagtag
     1621 ttaagagcct tattttaaaa agtttgtcca ttcttccaaa gataatcgta atcctcaata
     1681 aaaaaaggaa atagaactat taagatgtta acatttaata gtttatttcc tttttaagtt
     1741 atgaagaaaa gatcgttagg gtatttcggt cattgtcagt acaacagtcg aagtataagt
     1801 tccctggctg aaaataccgt cagacagttg tgaaaacatg ctggcggggt tcattgtccc
     1861 ttgcatggta ccgaacagcc agtgcaaaac gaacaggttg tcggtctgat ccccggaatt
     1921 gtccgttaaa aggtcgatca gggtgatatc agtattggaa agcccaaggg cggcatcggc
     1981 actttggtaa gcgcaggcca cctcggttcc gaattggtga acgccggtag cctcacacca
     2041 gactcccagg ttattgatcg gaatgacgcc tgttcccact atgggggtaa aatcaggtgc
     2101 gctgattttc aggtaccagt ttccggtggc ttccatcgcg acggttgtaa aaccgggatc
     2161 gatacccgga ttccccattg tttcggaaat acccaggttg tattcatcgg cagcgccaaa
     2221 tgtagctgtc tggacatctc catcctgcac gaccaggttg aaagctcctc ccaaaatggc
     2281 attgaaaact acttcctcgt cgtcggtcag ttgtgctgtt acactgttta attggatggc
     2341 agtgaaacag ataagtaaca gaattattct tttcataatt ttaaatctga tgttggaacg
     2401 aagggtgata atcgggtgtg aaaaacactt ccctggtaat gtctgcgtgg gaaacattca
     2461 tcatgaaaaa aggaaacatg gcgtaattgg tcccatgtcc ccttctttca tatgcgttaa
     2521 aattcttacg gaatttcggt cagggtcaga gtggctgtgg tcgtgaagtc accggtcgag
     2581 aacaaggtac cgagagccaa ctggttgaac atactttcag tgttcatgga tccctgcatg
     2641 gtacccatca gccagtgcag cgtaaaggcg ttgtcggaga tgtccccggc gttggttgtt
     2701 cccagtccga ttagcaattg gtcggcattg gtaagaccca tggctgcagc agcattctgg
     2761 tatgcgcagg tcacttcggt gccgaattgg tgagtacctg tagcctggca ccatacaccc
     2821 aggttgttga tcgggatagc accggttccg gggttaggac caccacccgg tgtaaaatcg
     2881 ggggcgctga tctccagttc ccagtcgccg gttgcttcga cgctgatcag ggtaaatccc
     2941 ggaacaatac cgccggcttc cgtgacaccg ttgttgtaat cggccgcaac ggcgaatgta
     3001 atttcttgtg taacaccgtc aacgttcagg ttgaaagttt cattgagatg agcattgaac
     3061 acaaccacct cctggtcggt caattgagcc ataatggctg cctgaagtgc gaacagcaat
     3121 ccgaaaagaa gaacaatctt tttcataatt ttaattttaa gattaagatg atttattaac
     3181 aatttcttgg gaggtggtca ggttaatatt aatcagcatg ttgataaaag gttattccaa
     3241 acctgaaaaa atttaaccgg acactcctaa tttaaatcaa gtataaataa tgaactcttc
     3301 tgtaaaaaat ttaacgaggg aaaaatggat aggtttcaaa aaatcgcaga aatctcgaaa
     3361 aaaaaacgaa aacttaatat accgaaaggg tgagaataat gtttaaggta tatgaatctg
     3421 ccggaataag ctggtcgagc atgcgagtct ggttcatatt tccgttccgg gtgcccatct
     3481 cccagtcgag cgtaaaggtg ttttcaatgc catacccttt attagataag gtaccttttg
     3541 tcagaagagt cacatcggta tattccaggg ccaaaggtgc ggcctttgca taattcagga
     3601 tatgggtacc atcatccagg ttggtaccga tcgagttgac cgtaacaccg acattgttaa
     3661 gctccatctg gtgggcaggg tcaaaggtgc cgtaaaacat atcctgatcg gctttaaact
     3721 ggagtttcca gtcgtatatt gcaccgatac ggataaatgt cgactggccg gcattctgaa
     3781 tcccgttgat gtattcatcg atctcatcaa aaacaaactg gatgctgtac ccggt
//
LOCUS       DAOQXS010000297         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24566_length_3829_cov_3.028352, whole genome shotgun sequence.
ACCESSION   DAOQXS010000297 DAOQXS010000000
VERSION     DAOQXS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24566_length_3829_cov_3.028352"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2752)
                     /locus_tag="PKM34_08575"
     CDS             complement(<1..2752)
                     /locus_tag="PKM34_08575"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNQ83683.1"
                     /translation="MSCRNLLFTVLPVFLFLELNAQVFINEGSNRNYSLIADENGEFP
                     DWVELYNAGNETISLYNYSLSDNIDEPGKWTFPNVDIGPGEYRVVFCSGKDRKPVTGF
                     VNVAHTGPFVAQTGWNIHTFNTPFYWDGISNILVNTCSYSSTGYTTNSVFNQTPTTYW
                     STVFNNQDGSDASCYAAYGGRVSQRPNMKLNGHAVGTGQVQNSPYDYPAPYGNWYWGA
                     RHQMLIRASELQAAGLPVGNITSLGFDVVSTDPNTTYDYIDISLKLVTVSEITQEFTP
                     VDTNNFLHTNFKLKKNGETVYLFSPGHTLLGSLSIGCGDLDNSLGRQPDAGSETYLFQ
                     AGTPGASNNASATFTDYLEPPSFSIPSGFFNQPFSVSITNPNPGQTSVHYTIDGSEPN
                     ILSPLYTGQPIEVMYSIVLKAKAFGWGILPSRNTVASYLFGVNHTTPVISVVTDDDNL
                     YGERGIFDNWWTDWERTAYVEYFDSLQNLIFSQRTGMQIDGGWGGARYQPQHSFRLEL
                     DDGVLGDGPVEYPIIPDRPERNKYGQIYLRNGSNQYLVLPYKDACQVTAMGTGTYNYY
                     SAWRPVSVYMNGYYFGLYELREKIDAEYFEVYDDADNDEMDLLSLSAWYGFAFRALEG
                     SVDNFFEDYYYFSLLEPSDTGYWKQADNYFDLKYYTDYIIGESWMANNDWPGNNIKIY
                     RSDKTNYRWRFCLIDLELGMAPNSWTDCYTDHIRYMLDQDPNNPFINIWKRSIENEQY
                     RHYFINRFADVMNTAYLAENIISLENRMFDQVVGEMPKEYARWGDANHIPEQMLTFTE
                     NHNIFVSQLAERSTQVRNHIQSNFQLPNQVLVTLGVYPEGAGKINISTIAPDEYPWQG
                     VYFNGVPVRIEALANQGYEFIEWDNNLLLNDTLNPVFQGMLDAESVSFNAIFEEISIG
                     VAS"
     gene            2916..3659
                     /locus_tag="PKM34_08580"
     CDS             2916..3659
                     /locus_tag="PKM34_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GW dipeptide domain-containing protein"
                     /protein_id="HNQ83684.1"
                     /translation="MIKKISLYSLFIAITAVSCNFGQKPDEYAHQKAKVREVIQTTNY
                     TYLRVEKSKQDQWIAIGKSDIKEGSVIYFEDGLQMVNFESPELGRTFESILFVQQISD
                     KPIKHEDFHGEMPEGMGEMGGMDPAMMGDKPSKPVIDKMEINLEHPAGAIKIGDLYAR
                     RSEYAGKQVTVHGKVIKVNVGIMGKNWLHIQDGTSDGDNFDLTITTIYEPAVGAVVTY
                     SGTLAVKKDFGYGYFYEIIVEDAEPVKAL"
     gene            complement(3654..3815)
                     /locus_tag="PKM34_08585"
     CDS             complement(3654..3815)
                     /locus_tag="PKM34_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNQ83685.1"
                     /translation="MPPNLVRLCVGAEHPDDIIADLEQALAVLDGKKVSVTSPEYSAG
                     GASSARIRS"
BASE COUNT         1057 a          892 c          894 g          986 t
ORIGIN      
        1 gagaagcaac cccgatggaa atttcttcaa aaatggcatt gaaactgaca gattcggcat
       61 ccagcattcc ctggaatacc gggttcaatg tatcgtttaa caggagattg ttatcccatt
      121 cgataaattc atacccttga ttggccagtg cttcgatccg gacaggcact ccgttgaaat
      181 agaccccctg ccagggatat tcatcgggtg caatcgtgct gatattaatt ttccccgccc
      241 cttccggata aacccccagg gttacgagca cctggttggg caattgaaaa ttcgactgga
      301 tatggttcct cacctgtgtt gaccgctctg caagctggga aacgaaaata ttgtgatttt
      361 ccgtaaaggt cagcatctgt tcagggatat ggttagcgtc tccccaccgc gcatattctt
      421 taggcatttc gcccacgacc tgatcaaaca tccggttctc aagcgaaata atattttcag
      481 cgagatacgc ggtattcatc acatcggcaa accggttgat gaaataatgg cgatactgtt
      541 cgttttcaat acttcgcttc cagatattga tgaacggatt attcgggtct tgatccagca
      601 tataccttat atggtcggta tagcaatctg tccagctatt cggggccatc ccgagttcaa
      661 ggtcgatcag gcagaatctc caccggtaat tagtcttatc cgaccggtaa atcttgatgt
      721 tattcccggg ccagtcgtta ttggccatcc acgactcacc gatgatataa tcggtgtaat
      781 atttcagatc gaaataattg tccgcctgtt tccagtatcc cgtatccgaa ggttcaagga
      841 gggaaaaata gtagtaatct tcaaagaaat tatcgaccga gccttccagt gcccggaaag
      901 caaatccata ccaggcgctc agcgacaaca ggtccatttc gtcattatcg gcatcatcgt
      961 acacttcaaa atattcagca tcaatctttt ctcttaattc atataaacca aaataataac
     1021 cgttcatata aactgatacc ggcctccagg cggaataata gttataggtt cctgttccca
     1081 tagccgttac ctggcaagca tctttataag gtaataccag gtattggtta ctcccgttgc
     1141 gaaggtagat ctgaccgtat ttgtttcgtt caggcctgtc gggtatgatc gggtattcca
     1201 ccggcccatc gccaaggacc ccgtcatcca gctccagccg gaaagagtgc tggggttggt
     1261 atcgggcacc tccccacccg ccatcgatct gcatccctgt tcgttgcgaa aaaatgaggt
     1321 tttgcaggga gtcaaaatat tcaacatagg ctgtccgctc ccaatcggtc caccagttat
     1381 cgaaaatccc tctttcaccg taaagattat catcatctgt gacgacactg atcacggggg
     1441 ttgtatgatt taccccgaaa agataagagg ccacggtatt ccggctcggg aggatgcccc
     1501 atccgaaggc cttggctttc aaaacgatgg agtacattac ttcaatcggt tgaccggtat
     1561 acagaggcga tagaatgttc ggctcgctcc cgtcgatggt gtaatgaacc gatgtctgac
     1621 cgggattggg attggttata gacacactga agggctggtt gaagaaaccg gaaggaattg
     1681 agaatgaggg cggttccaga taatcggtaa aagtggccga ggcgttattg gaagctcccg
     1741 gtgtaccggc ctgaaaaaga taggtttcgg aaccggcatc gggctggcgg cccaggctgt
     1801 tatccaaatc gccgcacccg atcgaaagcg aacccagcaa ggtatggccg ggagaaaaca
     1861 ggtaaaccgt ctcgccattc ttctttaatt taaaattcgt atgcagaaaa ttattggtat
     1921 cgaccggagt aaattcttga gttatctcac tgacagtcac taatttcaaa cttatatcga
     1981 tataatcgta agttgtattg ggatcggtgg atacgacatc aaatcccagg ctggtgatat
     2041 ttcccaccgg taaaccggcg gcctgcagtt cggaagcccg gatcaacatc tggtgacgcg
     2101 cgccccaata ccagtttcca taaggggccg gataatcata cggagaattc tgtacctgtc
     2161 ctgtacctac cgcatggccg ttcagtttca tgttcggacg ctggctcacc cggccaccgt
     2221 atgccgcgta acaagatgcg tcgcttccgt cctggttatt gaataccgta gaccagtagg
     2281 tcgtaggggt ctggttaaat accgaatttg tcgtataacc ggttgaacta taagagcatg
     2341 tgtttaccaa tatattcgat attccatccc agtagaaagg cgtgttaaac gtgtggatat
     2401 tccatcctgt ctgagcaacg aacgggcctg tatgtgccac gttcacaaat cctgtcaccg
     2461 gtttgcggtc tttaccactg caaaacacaa ctctatattc tccgggtcca atatcaacat
     2521 tgggaaaggt ccatttaccg ggctcatcta tattatcact caacgaatag ttgtacaggc
     2581 taatcgtctc gtttccggca ttatacaatt caacccaatc ggggaattca ccattttcat
     2641 cggcaattaa tgaataattg cggttactgc cttcgttgat aaaaacctgg gcgttcaatt
     2701 caagaaacag gaaaaccggg agaactgtga aaaggagatt tcggcaggac atagagaaat
     2761 ttttgataaa gttaaaactt tatggaaaca gaagaccaat tatgatgtat tgctttaaat
     2821 ggcattgcag ggctcaaaaa taaacataca atattcaaaa tgtattgata tttttgcatc
     2881 cgaaagaata gttcaatcct aaaatctcat tcatcatgat aaaaaagatc agtctatatt
     2941 cgctgttcat cgctattaca gccgtttcat gcaatttcgg gcaaaagccc gacgaatatg
     3001 cacaccagaa agctaaagtc agggaagtga tccaaacgac caactacaca tatctcaggg
     3061 ttgagaaaag caaacaggat cagtggattg ccattggcaa atcggatata aaagagggtt
     3121 cggtcatcta ttttgaagat ggcctgcaaa tggtcaattt tgagagcccg gaactgggcc
     3181 ggactttcga gagtatttta tttgtgcagc aaatcagcga caaaccgata aagcatgagg
     3241 attttcatgg cgaaatgcct gaaggtatgg gtgaaatggg tggaatggat ccggcaatga
     3301 tgggggataa accctccaag ccggtcattg ataaaatgga aataaatctg gagcatccgg
     3361 ccggtgcgat aaagattggc gatctgtatg cccggagaag cgaatatgcc ggaaaacagg
     3421 ttaccgtaca cggaaaggtt atcaaagtca atgtcggcat tatgggcaag aactggttgc
     3481 atatccagga tggaacctcc gacggcgaca atttcgacct gaccatcact acgatctacg
     3541 agccggcagt cggggctgtc gtgacctata gcggcacact ggccgttaaa aaagactttg
     3601 gttacggata cttttatgag attatcgtag aagatgccga gccggtaaag gctttatgat
     3661 ctgatccttg ccgagctcgc tccccctgca ctatattccg gcgaagtcac ggataccttt
     3721 ttcccatcca gcacggccag tgcctgttcc agatctgcaa taatatcatc cgggtgctcg
     3781 gcgccaacgc acaaacggac caggtttggc gggatgtcgg cgattttcc
//
LOCUS       DAOQXS010000298         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24679_length_3817_cov_4.162148, whole genome shotgun sequence.
ACCESSION   DAOQXS010000298 DAOQXS010000000
VERSION     DAOQXS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24679_length_3817_cov_4.162148"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKM34_08590"
     CDS             <1..479
                     /locus_tag="PKM34_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83686.1"
                     /translation="YIGTDVGVYYRDDDIGDWVPFMNGLPAVMVFDLEINESAGVITA
                     ATYGRGFWRSELYSSCPTSYFLDQANDPGNPNYTGFQHYEASSTVISSRIITGGIGTD
                     VTYQAGTYVLLETGFHARAGNKFQAVLGPCSGTAPPAASPDRVLKVTGTYSGQKID"
     gene            805..1785
                     /locus_tag="PKM34_08595"
     CDS             805..1785
                     /locus_tag="PKM34_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83687.1"
                     /translation="MKSLKITFGFFALVSFLGLNEAVGQWAPNGSHIYNTNAGNVGVG
                     YNTPASLLHVGKNMTEPTIRVQNFGGAGGATFQMIDNASTADWKFKATNTGGFKIRDN
                     AFAMDVITVEPNSLANAFYIDAAGFIGMGTNAPSDNLHIISDSYPFIFVEGTLAGGNQ
                     GIAFRENSVTRGWIYYSGSADGVRINAHSSGGSRNDIFIKSDGRVCIGTTTAATGYAL
                     SVNGKAACTEVLVDNTADWPDYVFSDEYDLMSLEELEQSIKENNHLPGIPSATEVEEN
                     GILLGEMQKNLLEKVEELTLYTIMQDKQIKELQMKIEALEKSKSGKGKNR"
     gene            1853..2242
                     /locus_tag="PKM34_08600"
     CDS             1853..2242
                     /locus_tag="PKM34_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83688.1"
                     /translation="MKTIKYMFLFLFVCVFADVFAQQENKTEGITKTGTGEGSPALTN
                     TPANRPPEGHIIPSVVTPALNGTVQELVKNDLNRVDEPNAASPDPKATSSGSVFEGEI
                     KDRETGIKEDNEPNAASPPKGDIPEIQ"
     gene            2261..>3817
                     /locus_tag="PKM34_08605"
     CDS             2261..>3817
                     /locus_tag="PKM34_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83689.1"
                     /translation="MKKNDRNIKISLFLVFFLVMGLYWSSNAQVGQSSDFVDPYSIWL
                     NPGTDDFATIQQNVESYYANIDRSKKGTGYKQWKRWEYIQQNRLSPDGKIINYAARNL
                     EEFDTYYKGSGSREITATTYGYWGSLGADYFVDGNGWNGGIGRVNCITFHPSVANTFW
                     VGTPSGGLWRTTTGGTSWTPLTDGMPRIGVSGLAVNYNNTNIMYLLTGDGDGGDVSSI
                     GVMKTTDGGVTWYPTGLTWAATDFERGYKILMHPTNPAILFVVSTAGIFRTANSGTTW
                     TNVHSTAGSNYHDIEFKPGDPSIVYACAGSKFYRSLDGGVTWTQITSGVPTTATRMAI
                     GVSPNNDAYVYLFAGPSYTSGVFVGMYLSTSSGSTFSTRSTTPNILGYSSTGADDDDQ
                     TTYDHAIAVSRTDVADVIIGGINTWSSTNWGATWTLTSMWNNAPGPDYTHADIHALEI
                     NPLNNYLYCGSDGGIFRSTDFGANWTDLSDGLEITQSYRIAGYEPNEDLITNGTQDNG
                     SNKWSGGTTFL"
BASE COUNT         1067 a          868 c          913 g          969 t
ORIGIN      
        1 tctacatcgg taccgatgtc ggtgtttatt acagggatga cgatatcggc gactgggttc
       61 cgtttatgaa tgggcttccg gccgtaatgg tatttgatct ggaaatcaat gaatcagccg
      121 gagttattac tgcagcaaca tacggacgtg ggttctggcg ttcggaactc tattcgagct
      181 gcccgacctc gtacttcctg gaccaggcta atgatccggg taatccgaat tatactggtt
      241 ttcagcatta tgaggccagc agtactgtca tctccagccg aattatcacg ggaggtatcg
      301 ggacagatgt gacttatcaa gccgggacgt atgttctgct tgaaacgggc ttccatgcca
      361 gagccgggaa caaattccag gctgttctcg ggccctgttc cggtactgcg ccaccggccg
      421 cttcccccga cagggtgttg aaggtcaccg ggacttattc cggtcagaag atcgattgat
      481 atatttgttt gtaaactcac tccaacgact caagccagtg gttccaagta aattaaaaaa
      541 ccggaaccga cggctttagt cgtcggaagg aaatctttca aaaaaaccga cggctttagt
      601 cgtcggacta ttaacgaaaa aggaggttgc tcatggcttc gggcaacctc ttttttcata
      661 tcttaatttt tccttaaaat tcttaagtaa ttgttaaaaa tttgatatgg atatatatcc
      721 atacctacct ttgatcaaac cctgaccatt aatctgtcat taataacttc tttataataa
      781 tttataaact aaaaacaatt gattatgaaa tctttaaaaa ttacttttgg attttttgct
      841 ttagtctcat tcctgggatt aaatgaagca gtcggacaat gggctccgaa cggttcacac
      901 atttacaata ccaatgccgg taatgtaggt gtcggatata acactccggc ctcccttctt
      961 catgttggta agaatatgac cgagcccacg atccgtgtgc agaattttgg cggagccggt
     1021 ggcgcaacct ttcagatgat cgacaatgcc agtacagccg actggaaatt caaagccacc
     1081 aataccggcg gttttaagat ccgcgataat gcttttgcca tggatgtgat caccgttgaa
     1141 ccgaacagcc tggccaatgc attttatatc gatgcagcgg gttttattgg catgggtaca
     1201 aacgcccctt ccgataatct tcatatcatc agcgattctt atccgtttat tttcgtcgag
     1261 ggtacactag ccggaggaaa tcagggaatt gcctttaggg aaaatagcgt aacaagagga
     1321 tggatttact acagtggttc tgctgacggt gtgagaataa acgcacattc cagcggcgga
     1381 tcgcgtaatg atattttcat caagagcgat ggcagggttt gtatcggaac gactactgct
     1441 gcgaccggat atgctttgtc ggtcaatgga aaagccgcat gtaccgaggt attggtagat
     1501 aacacagccg attggcccga ttatgttttc tctgatgagt atgatctgat gagccttgaa
     1561 gaacttgagc agagcattaa agaaaacaac caccttcccg gaatcccctc agccactgaa
     1621 gttgaagaaa atggaattct actgggtgaa atgcagaaaa acctcctgga gaaagttgag
     1681 gagcttactc tttatacgat aatgcaggat aaacagatca aagaacttca gatgaaaatt
     1741 gaagctctgg aaaagagcaa atcaggaaaa ggtaaaaacc gctaattatc cctgaaatat
     1801 ttagtacagg aaggttagta ttatcctcat tataaatttt aatctgaaga atatgaaaac
     1861 aataaaatat atgttccttt tcttatttgt ctgtgttttt gcagatgtgt ttgcccagca
     1921 ggaaaataag acagaaggaa ttactaaaac agggacagga gaaggttcac ccgcattgac
     1981 caatacgccg gcaaatcggc ctcctgaagg ccatatcatt ccatccgttg taactcctgc
     2041 cctcaatgga acagtacagg agctcgtaaa gaacgattta aaccgggttg atgaaccgaa
     2101 tgcggccagc ccggatccga aagcaacttc atccgggtct gtgttcgaag gtgaaataaa
     2161 agacagagaa accggcataa aggaagacaa cgaaccgaac gcggcaagtc cgccaaaagg
     2221 tgatatcccc gaaatccaat aattcttaaa aatcaggact atgaaaaaga atgacagaaa
     2281 catcaaaata agtctatttc tggtattttt cctggtaatg ggattatact ggagttcaaa
     2341 tgcgcaggtc ggccaatcct ccgattttgt cgatccatac tcaatctggc tgaaccctgg
     2401 taccgatgat tttgcaacca ttcagcaaaa tgtcgaaagc tattatgcca atattgatcg
     2461 atccaaaaag gggaccggat acaagcaatg gaaacgctgg gaatacattc agcagaaccg
     2521 tctttctccc gatggaaaga tcatcaacta tgcagcccgg aatctggagg aattcgatac
     2581 gtattataaa ggaagcggtt caagggagat tacggcaacc acttatggtt actggggttc
     2641 tctcggagcc gactactttg tcgatggcaa tggctggaac gggggaatcg gacgggtgaa
     2701 ctgcatcaca tttcatcctt ccgtcgccaa tactttctgg gtggggacac catccggcgg
     2761 gctttggaga accaccaccg gaggtacaag ctggacaccg ctgaccgacg ggatgccgag
     2821 aatcggcgtg agcggtttgg cggttaatta caacaacacc aatatcatgt atctccttac
     2881 cggtgatggc gatggaggag atgtcagctc tatcggcgtc atgaagacta ccgacggtgg
     2941 ggtaacatgg tatccgactg gtttgacatg ggctgcaacc gatttcgaga gaggatataa
     3001 gatcctgatg caccccacca acccggcgat cttatttgtt gtgagcacag caggtatttt
     3061 cagaacagca aactcgggta ctacatggac caatgtgcat tcaaccgccg gcagcaatta
     3121 tcacgatatt gaattcaagc cgggtgatcc gtcgatcgtc tacgcctgtg ccgggtcgaa
     3181 attttaccgg tcgctggatg gtggtgttac ctggactcaa atcaccagcg gggttccgac
     3241 cactgcaacc aggatggcca tcggggtttc ccccaataat gatgcctatg tttacctgtt
     3301 tgccggtccg tcttatacca gcggtgtttt tgtcggaatg tacctttcca cctccagtgg
     3361 atctactttc tcaacccgct cgaccacacc gaatatactc ggttacagtt ccacaggggc
     3421 cgacgatgat gaccagacta cctatgatca tgccatcgcc gtttcaagga ccgatgtcgc
     3481 ggacgtaatc attggaggga tcaatacctg gagttcgaca aactgggggg ctacctggac
     3541 tttgacgtcg atgtggaaca atgctcccgg tcctgactat acacacgccg atattcatgc
     3601 acttgagatc aacccgttga ataactatct ctattgcgga agtgacggag ggatattccg
     3661 gtcgaccgat ttcggagcaa actggaccga cctgtcggat ggcctcgaga tcacccaatc
     3721 ataccggatt gccggttatg agccaaatga ggacctgatc accaacggca cccaggataa
     3781 cggttccaac aagtggtcgg gagggacaac tttcctt
//
LOCUS       DAOQXS010000299         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24849_length_3798_cov_4.216671, whole genome shotgun sequence.
ACCESSION   DAOQXS010000299 DAOQXS010000000
VERSION     DAOQXS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24849_length_3798_cov_4.216671"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKM34_08610"
     CDS             <1..721
                     /locus_tag="PKM34_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83690.1"
                     /translation="IAETLGRTIPAKEHLFTSENVIPDLLKKMADKRNTVTHFVDPEI
                     VSHEEMQGFRYIEHRENVSLALAVCLHAGVDRNTALQGMYKAVPDAGALMVFRVEAFR
                     KNLVFYNAFAANDPDSTFLVWRKISDETGMEGKRIVLLNTRQDRLYRAEQLAGMVAYR
                     LEKEVDYLVLIGQSTDVVESMAIRYGIPARKVVTVGWTTSENVFDRLLALTDHTATIV
                     AIGNMGGMGSGLVEFFEHRSI"
     gene            725..1177
                     /gene="pgsC"
                     /locus_tag="PKM34_08615"
     CDS             725..1177
                     /gene="pgsC"
                     /locus_tag="PKM34_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HNQ83691.1"
                     /translation="MIELAITLGLVLSLLSYEVFGLAAGGIVVPGYIALQLSHPDRLI
                     GILVVSLVTYLIIKVLGNYTFLYGRRQMVLSLLIGCLLAIFTRHFLTFNLGSSTLELE
                     AVGWVIPGLIAHWFAKQGIFKTISVLFVSSVLVRLVLILIFAGALLPK"
     gene            1186..2343
                     /gene="pgsW"
                     /locus_tag="PKM34_08620"
     CDS             1186..2343
                     /gene="pgsW"
                     /locus_tag="PKM34_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNQ83692.1"
                     /translation="MFKFKATSNLVLGLLFLLALLAFIAVENGKKDVKQDYYNEKLEA
                     ARLSQAAAGHIKQYRLQKGVFLDDVNDPNQTALIGQEYTMITTDQGNVESKLSATNPN
                     FAAVIVQLLKEAGLEEGDPVAIAFSGSFPGMNISVMAAIETLKLQPTVITSVGASNFG
                     ANDPYFTWLDIETLLNDAGIFHYKSVAASIGGGNDIGRGLSPEGRNLIISAIERNRVE
                     LINEKHLENSIARRMEIYEQQGKKNAAKAFINVGGGIASLGNTVNGQLIPSGLTERLP
                     MSNYPLHGVIIQMGRKGIPIIHLLNIDQLAAKYGLPANPVPMPEPGEGGIFIQKKYDL
                     VVTTIALAILVTVIIFVYFAERKRHQLGTDVVPVVPGQRKQQAEDEMIEEI"
     gene            2406..3314
                     /locus_tag="PKM34_08625"
     CDS             2406..3314
                     /locus_tag="PKM34_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83693.1"
                     /translation="MMIKKFGVFGFLLLLTGSICAQTESKTIKPSGFVKKITTVSAGK
                     NRSYYALSYADAAMVTVQGPGVLKVHTRAQFRPGDQVQARYTVIYTVDGGTQKSVSVS
                     GIERSRNATYTDGTLGVPGELKTFEIELSRGFHTVEFRLKDITANVAARYVFTPAKLK
                     KQDWMAFSPLRPSEPVDLISRETTTPYYRFTAEKPLRVEIIGPTELRVLIRTEHHFGM
                     KVRINYRVQVRENGSVINTYQLNVKVSDVAVYKDIDNLIPGTACEFVILIPDGKHLYE
                     ICPLDQDKNSLLGRLLIPGKDVGLEE"
     gene            3327..>3798
                     /locus_tag="PKM34_08630"
     CDS             3327..>3798
                     /locus_tag="PKM34_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83694.1"
                     /translation="MRIFIQKYYRCCLPTYFLIILFLVFGMQTAAAQKKSSKKEKHFE
                     AEIKFGSMYDDNILKYSGKYIDRFINRQDSGRFHIKTYDDIVFITAADLSATYRIFKD
                     LKTRINGSIYSNMYATNGVKNWYYFTLGLTQNVTKKASFRVSYSHIPRFYVRHFR"
BASE COUNT          954 a          925 c          986 g          933 t
ORIGIN      
        1 aattgctgag acactgggtc ggaccatacc cgcgaaggaa catcttttca cctctgaaaa
       61 cgtgattccc gacctcctga agaaaatggc cgataaacgg aataccgtga cccattttgt
      121 ggatccggaa attgtttcgc atgaggagat gcaaggtttc aggtatattg aacaccggga
      181 gaatgtttcg ctggcgctgg ccgtttgcct gcatgccggc gtcgatagga atacggcgtt
      241 gcaggggatg tacaaagctg ttccagatgc gggcgccctg atggttttca gggtggaagc
      301 cttccggaaa aacctggtct tttacaatgc ctttgccgcc aatgatcccg attccacctt
      361 cctggtctgg cggaagatca gcgatgaaac gggaatggaa gggaaacgga tcgtgttgct
      421 gaatacccgg caggaccggc tgtaccgggc agagcaactg gccggcatgg tggcttaccg
      481 gctcgaaaag gaggtcgatt atcttgtgtt gatcgggcaa tcgaccgatg ttgtggagag
      541 catggctatt cgctatggca ttcctgcccg gaaagtcgta acggtcggat ggacaacatc
      601 ggaaaatgtt ttcgaccggt tgctggctct gaccgaccac acggccacca ttgttgcgat
      661 cggcaatatg ggcggaatgg gatcgggtct cgtcgaattc tttgaacaca ggagtatata
      721 atttatgatt gaattagcca taacccttgg cctggtcctc agccttcttt catacgaagt
      781 attcggactg gcggccggcg ggatagtcgt tccgggctat atcgccctgc aactgtcgca
      841 ccccgaccgg cttatcggaa tcctggtggt cagcctggtg acttacctga tcattaaagt
      901 gctgggcaat tacacctttt tgtatggcag gcgccagatg gtcctcagcc tgttgatcgg
      961 ttgtttgctg gctattttca cccggcattt cctgaccttt aacctgggaa gctcaacgct
     1021 tgaactcgaa gccgtcggct gggtcatccc ggggctgatc gcccactggt ttgccaaaca
     1081 gggaatcttc aaaacgatca gcgtactgtt tgtttcatcc gtactcgtcc gcctggttct
     1141 tatcctgatc tttgcgggcg ctttactgcc caaataggag gtgatatgtt taaattcaaa
     1201 gcaacttcga acctcgtgct gggcctcctc ttccttctgg ctttgctggc ttttatcgca
     1261 gtggagaatg gaaagaaaga tgtcaaacag gattattaca atgaaaaact tgaggcagcc
     1321 cggctctccc aggctgcagc cggtcatatc aaacaatatc gcctgcagaa gggtgttttt
     1381 ctggatgatg tgaatgatcc caatcaaacg gccctgatcg gacaggaata caccatgatc
     1441 acgaccgacc agggaaatgt cgaatcaaaa ttatctgcta ccaacccgaa ctttgccgca
     1501 gtgattgttc agttgctgaa agaggcgggg ctggaagaag gcgatccggt ggccatagca
     1561 ttctcggggt ctttccccgg gatgaatatc tccgtaatgg cggccattga gacgctcaaa
     1621 ctgcaaccaa ccgtgatcac ctctgtcggg gcatccaatt tcggagccaa tgatccctat
     1681 tttacctggc tcgatataga gacactgctg aatgatgcag gcatttttca ctacaaatcg
     1741 gtagccgctt ccatcggcgg cgggaatgac atcggccggg gcctgagccc cgaaggccgg
     1801 aatcttatca ttagcgccat tgaaaggaac cgggttgaat tgattaatga gaaacacctc
     1861 gaaaactcca ttgccaggag aatggaaatt tacgagcaac agggcaaaaa gaatgcagcc
     1921 aaggcgttta tcaatgtcgg cggagggata gccagcctcg gcaatacggt aaatgggcag
     1981 ctcatccctt cggggcttac cgaacgcctt cccatgagca attacccgtt gcacggtgtg
     2041 atcatccaga tgggccggaa agggatcccc atcattcacc tgctgaacat cgaccagctg
     2101 gcagcaaaat acggactgcc ggccaatccg gtgcccatgc cggaaccggg tgaaggaggc
     2161 attttcattc agaaaaaata tgatctcgtt gtaacgacta ttgctctggc catcctcgtc
     2221 accgtcatca tttttgttta ctttgcggaa cgaaaacggc accagctggg aacggatgtt
     2281 gtgcctgtcg ttccgggaca gaggaagcag caggctgaag atgagatgat tgaagagata
     2341 tgatggtcga ttgtcgatgg tcgatggtcg attttcgatt tgtattgaga gttttatttg
     2401 atagtatgat gattaagaaa tttggagttt tcggtttttt attgctttta accggcagta
     2461 tctgtgccca gacggaatcg aagaccatta agccttcggg atttgtgaag aagattacga
     2521 ctgtgagcgc cgggaagaac aggagctatt atgcgctgag ttatgccgat gcggcaatgg
     2581 ttaccgtcca ggggccgggg gtgctgaaag tccatacccg tgcgcagttc cggccgggcg
     2641 accaggtcca ggcacgctat acggttatct atacggtcga tggcgggact caaaaaagtg
     2701 tctcggtttc agggatcgaa cgttcacgga atgcaaccta caccgatggc acccttggcg
     2761 tcccggggga gctgaaaaca ttcgaaatcg aactttcgcg gggatttcat acggttgaat
     2821 tccggttgaa ggatattacc gccaatgtgg ctgcccggta tgtttttacc cccgctaagc
     2881 tgaaaaagca ggattggatg gctttttccc ccctgcgccc ttccgagccc gtcgatctca
     2941 tctcccgtga aaccaccacg ccttattacc ggtttacagc agagaaaccg ctgcgtgttg
     3001 agatcatcgg cccgaccgaa ttgcgcgtcc tgatccgaac ggagcaccat tttgggatga
     3061 aagtacggat caattacagg gtccaggtca gggaaaacgg atcggtcatc aatacctacc
     3121 agttgaacgt caaagtgtcg gatgtagccg tttataaaga tatcgacaat ctcatccctg
     3181 gaacggcctg cgaatttgtt atcctgatcc ccgacggaaa acatctatat gaaatctgcc
     3241 cgctcgacca ggataaaaat tctttgctcg gacgattgct gattccaggg aaagatgtcg
     3301 gactcgaaga ataaaaacaa ctgaaaatga gaatattcat acagaaatat tatcgatgct
     3361 gcttacctac ctactttctg atcatacttt ttttagtatt cggaatgcag accgcagccg
     3421 cgcaaaagaa atcttccaaa aaggagaaac atttcgaggc cgagattaaa ttcggctcga
     3481 tgtacgacga caatatcctg aaatattccg gcaagtatat tgatcgtttc atcaatcgcc
     3541 aggattccgg caggtttcac atcaagacat acgatgatat cgtgttcatc acggctgccg
     3601 acctgtctgc aacataccgg atcttcaaag accttaaaac ccggatcaac ggaagcattt
     3661 acagtaacat gtatgccacc aacggcgtga aaaactggta ttatttcacc ctggggctca
     3721 cacaaaatgt caccaagaaa gccagcttca gggtttccta ctctcacatt ccccgctttt
     3781 atgtgaggca ctttaggg
//
LOCUS       DAOQXS010000300         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24930_length_3789_cov_3.091859, whole genome shotgun sequence.
ACCESSION   DAOQXS010000300 DAOQXS010000000
VERSION     DAOQXS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24930_length_3789_cov_3.091859"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKM34_08635"
     CDS             <1..613
                     /locus_tag="PKM34_08635"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNQ83695.1"
                     /translation="DRSAFSTSLGLEDQALTAMIARIDPGFPIFTLDTGRLFAETYDL
                     IDLTSLRYNVKIKVYFPDNQKVEEMVSSRGINLFYDSVENRKLCCHIRKIEPLQRALT
                     GVDFWISGLRREQSVTRENLTLAQYDPLHHKIKINPLINWTYEQVQSFIKINKVPYNP
                     LHDKGFPSIGCQPCTRAVEPGEDIRSGRWWWENPEMKECGLHK"
     gene            complement(620..1846)
                     /locus_tag="PKM34_08640"
     CDS             complement(620..1846)
                     /locus_tag="PKM34_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ83696.1"
                     /translation="MNLPSFIANRISGKDKENLSGPAVRVSVFTIALGLAIMILAVSV
                     LVGFQKEIRNKVIGFSAHIQIDNFDDNTSFESQPIPADQTFYSDLKATPGIRHIQVFG
                     LKAGIIKTRDQLQGVILKGVGPDHDWDFMKNHLAEGEIITLEDSAASDGILISRLLAS
                     KLKIITGDEIRMYFISGSDSQPRGRKFTVTGIYETGLEEFDELYVIGDIRHIRRLNNW
                     EPGMVSGFEVFIDDFENIDDLGEKVYQTIGYDLNAETIVQKYPQIFDWLRLMDINVVI
                     ILVLMILVAGITIISTLRILILERTSMIGILKALGTKDRDLRKMFIFLTGRIIARGLI
                     WGNLAGVGIGLLQYYFRIIPLDQESYYMAYVPVSLILWQILLINLVTVVTCLVMVLIP
                     GYIIGRITPVKAIRYR"
     gene            1937..3154
                     /locus_tag="PKM34_08645"
     CDS             1937..3154
                     /locus_tag="PKM34_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNQ83697.1"
                     /translation="MMIHQYILIVLSILWMAFSGIQTGNAQPSQSTAIRQVPYTAIRT
                     GAERSDVYVPLLKGKRVGIVCNQNSRIGTTHLVDYLLESGVKIEKIFAPEHGIRGDVE
                     AGGTIDHGTDERTGIRIVSLYGSNKKPAQQDLAGLDLIIFDIQDVGVRFYTYISTLAL
                     VMEAAAENDLPVVVTDRPNPNGFYTDGPVLDTNFKSFVGMHPVPVVYGMTIGEYALMV
                     NGEGWLGSRKCELTVIPMEGYDHNMLVTLPFRPSPNLTTWESIYLYPSTCFFEGTIVS
                     VGRGTLGPFQVFGHPGFLIGSYKFTPVKIPGMAEKPLFEGEECLGHEVTEYAHDFLNH
                     PNQLNLFWLIESYRYFSGRADFFNSYFDKLAGTDRLRLDILGGTGEDEIRKSWEPGLS
                     DFRAIREKYLIYP"
     gene            complement(3165..>3789)
                     /locus_tag="PKM34_08650"
     CDS             complement(3165..>3789)
                     /locus_tag="PKM34_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ83698.1"
                     /translation="KKYDLYLITDEVYREFVYDGHHHYSAMHLEGIEQNVILIDSVSK
                     RYSACGFRVGCLITRNKDVARTALKFAQARLSPPTFGQVAAEAAIDTPSEYFVRVLDE
                     YHARRNIVVDYINKIPGGYCPNPKGAFYVVARLPIDDSDRFCKWLLEDFNFNNETVML
                     APATGFYSNPSQGKDEVRISYVLKVEDLKKAMVCLEEALKVYPGRTL"
BASE COUNT         1001 a          928 c          910 g          950 t
ORIGIN      
        1 ggacaggtca gctttttcca ccagcctcgg cctcgaagac caggccctga ccgccatgat
       61 tgcccgcatt gatcccggtt tcccgatatt tacgctggat accggacggt tgttcgcaga
      121 aacgtatgac cttatcgacc tgacatcttt aagatataat gtaaaaatta aagtttattt
      181 tcctgataat cagaaggtag aagagatggt ttcaagcaga ggcatcaacc tgttttacga
      241 cagcgttgag aaccgaaaac tctgctgcca tatccggaaa attgagccgc tccagcgggc
      301 cctcaccggc gttgacttct ggatcagcgg cctgagaaga gaacagtcag ttacccgtga
      361 aaaccttacg cttgcccaat acgatcctct tcatcataaa attaaaatca acccgctgat
      421 caactggact tacgagcagg tgcaaagctt cataaagatc aataaagtcc cgtataatcc
      481 attgcacgac aaggggtttc caagtatcgg atgccagccg tgcacccgcg ctgtcgaacc
      541 cggggaagat atccgcagtg gccggtggtg gtgggaaaat cctgagatga aagagtgcgg
      601 cttgcacaaa taaccggttc taccggtagc ggatcgcttt taccggtgta atcctgccaa
      661 tgatgtaacc cggaatcaga accatgacaa ggcaggtgac cacagtcacc aggtttatca
      721 ggagaatctg ccataatatg agggatacag gcacataggc catgtaatag gattcctggt
      781 ctaacggaat gatcctgaaa taatactgca acaaaccgat accgaccccg gccaggttgc
      841 cccagatcaa tcctctggcg atgatccggc cggtaaggaa aataaacatt ttgcgcaggt
      901 cccggtcctt tgtacccagc gccttcagaa ttccgatcat gcttgtcctt tccaggatca
      961 gtatccgcaa ggtggagatg atcgtaatcc cggctacaag gatcatcagg acaagaatga
     1021 tgaccacgtt gatgtccatc agccttaacc agtcgaatat ctgcggatat ttctgcacga
     1081 tggtttccgc attcaggtca taacctatgg tttgatagac cttttctccc agatcatcaa
     1141 tattctcaaa atcatcaata aatacttcaa atcccgacac cattcccggc tcccagttat
     1201 tcagccgccg gatatgccgg atatcgccga tcacatacaa ttcgtcgaat tcttcgaggc
     1261 ccgtttcata aatacctgta accgtaaatt tcctccctct gggttgtgaa tcggagccgg
     1321 agataaagta catcctgatt tcatctccgg tgataatttt cagctttgaa gccagcagcc
     1381 ttgaaatcag gataccgtcg gaagcggcag aatcttccag ggttattatt tccccttcgg
     1441 ccagatgatt tttcataaaa tcccagtcat gatcagggcc aacccctttc aggatcacgc
     1501 cctggagctg atcccgggtt ttaatgatac cggctttaag gccgaagacc tggatatgcc
     1561 ttattcccgg cgttgctttc agatcggagt aaaatgtctg gtcagccggg atcggttggg
     1621 attcaaagga ggtattgtcg tcgaagttat cgatctggat gtgggctgag aatccaatca
     1681 ctttgtttct gatctccttc tggaaaccca caaggacgga cacagccagg atcattatag
     1741 ccagaccgag agcaatggta aatacagaga cccggacagc cggccccgac aaattttctt
     1801 tatctttacc gctgatgcga ttggctatga atgagggcag attcaaaact attcacgaat
     1861 taatcggtaa aaataagaac gaataacggt ttgattgcat ttcatgataa ctttaagaaa
     1921 aacaagggca atcaggatga tgatccatca atatatttta atagtccttt cgatcttatg
     1981 gatggcattc tccggtattc agacgggaaa tgcacagcca tcgcagtcga ctgcgatccg
     2041 gcaggtacca tacacggcca ttcgaaccgg agctgaacgg tctgatgttt acgttccatt
     2101 actgaaagga aaaagggtgg ggattgtctg caatcagaac tcgcgcatcg gaacaactca
     2161 tctagttgat taccttttag aaagcggcgt gaagatcgaa aaaatatttg caccggagca
     2221 cggtatccga ggcgacgtgg aagctggcgg caccatcgat cacggcaccg atgaaagaac
     2281 gggcatcagg atcgtttccc tttatgggag caataaaaag ccagcgcaac aggacctggc
     2341 gggtcttgat ctgattatct ttgacataca ggatgtcggc gtaagatttt acacctacat
     2401 ttccaccctg gccctggtca tggaggctgc cgctgaaaac gatctgccgg tggtggttac
     2461 cgaccggccg aatccgaatg gattttatac cgatggcccg gtgctcgaca cgaactttaa
     2521 atcatttgtt ggcatgcatc cggtgcctgt tgtctatgga atgactatcg gagaatatgc
     2581 gctgatggtc aatggagaag gatggcttgg ttcgaggaaa tgcgaactta cagtgatccc
     2641 catggaaggc tatgatcaca acatgctggt aaccctgcca ttcaggccct cacccaatct
     2701 gaccacatgg gaatcgattt acctgtatcc ttcaacctgc ttctttgaag gcaccatagt
     2761 cagtgtcgga agaggcacct tggggccttt ccaggtattc ggccaccccg gttttttaat
     2821 tggcagttat aaatttaccc cggtgaagat tccggggatg gctgaaaaac cactgtttga
     2881 aggagaggaa tgcctggggc acgaggtgac tgagtacgcc cacgactttc tcaaccaccc
     2941 gaaccaactg aatttgtttt ggctgattga atcgtaccgg tatttctcag ggagggccga
     3001 cttcttcaac tcctattttg ataagctggc cggtaccgac cggttaaggc tggatattct
     3061 cggaggaaca ggcgaagatg aaatccgaaa atcctgggag ccaggactct cagatttcag
     3121 agctataaga gaaaaatacc ttatttatcc ttagcgggaa atgatcagag cgtccgtccg
     3181 ggatacactt tcaaagcttc ttccaggcac accatcgctt tcttcaagtc ttccacttta
     3241 agaacatagg aaatccttac ttcgtcttta ccctgtgaag gatttgaata gaagccggtt
     3301 gccggtgcca gcattaccgt ttcgttgttg aaattaaaat cttcgaggag ccatttgcag
     3361 aaccggtcgg aatcgtcgat cggcaagcgg gccaccacat aaaacgcccc tttcggattc
     3421 gggcagtaac ctcccgggat tttgttgata taatcgacga cgatattgcg ccgggcgtgg
     3481 tattcatcaa gtaccctgac aaagtattcc gaaggcgtgt cgattgctgc ttcggctgcc
     3541 acctggccaa aggttggcgg gctcagcctg gcctgtgcga acttcagtgc tgtccgggca
     3601 acgtctttgt tccgggttat gaggcatccc acgcggaagc cgcaggccga atagcgtttt
     3661 gagaccgagt cgatcaggat cacattttgt tcaatacctt cgagatgcat tgcggagtaa
     3721 tggtgatgtc catcatacac aaactccctg taaacctcat cggttatcag gtataaatca
     3781 tatttcttc
//
LOCUS       DAOQXS010000301         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_24955_length_3787_cov_3.841640, whole genome shotgun sequence.
ACCESSION   DAOQXS010000301 DAOQXS010000000
VERSION     DAOQXS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24955_length_3787_cov_3.841640"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKM34_08655"
     CDS             <1..705
                     /locus_tag="PKM34_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNQ83699.1"
                     /translation="EEWLPDEVMQKIAMENNLSETAFYIRKEEGYHIRWFTPKVEVNL
                     CGHATLASAHIIFQTTGISDDTIPFQSRSGLLNVRKEGDLLVLDFPANKPQRTGLPED
                     FIHSLNITPIQCHRGKEDYLLLYKTQQEIEALLPDFKRLEKTDARAVIVTAPGNHVDF
                     VSRFFAPRVGVDEDPVTGSAHTVLIPFWAEKLGKNEMKALQLSRRGGSIFCRLRDDRV
                     DIGGKAVTYLKGEITI"
     gene            complement(802..1614)
                     /locus_tag="PKM34_08660"
     CDS             complement(802..1614)
                     /locus_tag="PKM34_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ83700.1"
                     /translation="MNDPHSNEMELKKTAVVTGATGAIGKAIVRQLAASGNYNVIMVA
                     RDESKAIRVVKDIQAKTGSLDIVYELADLSEKVSIYELASRINRPVHVLINNAAFTPQ
                     RRIENSEGIEMQFAVNVMGYFRMIDALTPQLKAGAPARIVNVASYWAGGLDLDDPEFR
                     KRKYENDTAYRQSKQANRMLTVAFARKLMPYRITVNSCHPGDVNSALSNSLGFGGHES
                     PDQGADTPVWLATSRETQEITGKYFEHRKEAPCSFSQDSQKVGLLYEYCSCR"
     gene            1655..1822
                     /locus_tag="PKM34_08665"
     CDS             1655..1822
                     /locus_tag="PKM34_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83701.1"
                     /translation="MIIGLFIYSLIFIAVGILFALRKKKLIAAMFFLLALLLAVVGVA
                     AIVVYPHIWPF"
     gene            1824..2522
                     /locus_tag="PKM34_08670"
     CDS             1824..2522
                     /locus_tag="PKM34_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83702.1"
                     /translation="MKKPALILLMVLLTCSGITQVNPTFIGLKTGVSVPVGQFGGHDL
                     VTGSFTQAGFQGAIEGAWFFKPWIGAGLHGFAALHPIDVGTLGYEKVIADPFMDDVTI
                     RSEPFRTFGGYAGLFFHVPLVKNLSVTGKALGGMIYAKTPYQLYKAEYYLVGEQWFEI
                     TSAADYEASFLAGVGIRYDINKSTAFCLDTEFTWNKADFEFITTGNDIRIEEKDISFL
                     NIGVGVIWKISAGE"
     gene            complement(2537..3253)
                     /gene="pepE"
                     /locus_tag="PKM34_08675"
     CDS             complement(2537..3253)
                     /gene="pepE"
                     /locus_tag="PKM34_08675"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNQ83703.1"
                     /translation="MKRLLLISNSTNPGEPYLGWPRPYITSFLAGTGVKNILFIPYAG
                     VNLSPEGLEKSYDIYEQRVQAVFKELGYGIYSIHHEQDPVEAVRKAEAIAVGGGNTFY
                     LVYMMHKMKLVDVIRDRANDGMHYMGWSAGANVACPTLRTTNDMPIVQPDSFRTCDLV
                     PFQINPHYLDANPEGHGGETRQQRIEEFLAVNRDITVIGLRESSLIQVEGDKYELKGK
                     RPMRVFRYGLEPVETVSFDY"
     gene            complement(3288..>3787)
                     /locus_tag="PKM34_08680"
     CDS             complement(3288..>3787)
                     /locus_tag="PKM34_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83704.1"
                     /translation="VWLEVPAAYQEQEAGLERRLNGPWHDYLKAFEQLRNDYSRNKLL
                     DYGIAFAPLIERIVASTDPDFLKDALFFYIELKDADNSFRVLKHIYSLGIPADPLREM
                     QHKLGKLMAMRDYSVNGSEKPWTTMESRQVSDKWFRDLKWSYKRTLLEKNRWKMKYWP
                     VIWKN"
BASE COUNT          943 a          905 c          889 g         1050 t
ORIGIN      
        1 gaagagtggc ttcccgatga ggtaatgcaa aagatcgcca tggagaataa cctctccgaa
       61 acagcttttt atatcaggaa agaagaaggc taccatattc gttggtttac gccgaaagtg
      121 gaagtgaacc tttgcggtca tgcaacttta gcatcagctc acattatttt tcagaccacg
      181 ggcatctccg acgatactat tcctttccaa tccaggagcg gccttttgaa tgtcaggaaa
      241 gaaggagatt tgctggtgct cgacttcccg gccaataaac cgcaacggac cgggcttccc
      301 gaagatttca tccattccct gaatatcact cctatccaat gccatcgggg caaggaagat
      361 tatctgctgc tgtataaaac gcagcaggaa attgaagcac ttttaccgga tttcaagcgg
      421 ctggagaaaa cggatgcgcg ggctgtaatc gtaacagctc ccgggaacca cgtcgacttc
      481 gtatcccgtt tctttgctcc ccgtgtgggt gttgacgaag atcccgttac cggctctgcc
      541 catacggttc ttatcccctt ctgggccgaa aagcttggga agaatgaaat gaaggccctg
      601 cagctttccc gccgcggggg aagcattttc tgccgcctgc gcgacgacag agtagatatc
      661 ggcgggaaag cggtgactta tctgaaaggg gaaattacaa tttagttcaa agtgcaaagt
      721 gcaaagtgca aagtgcaaag ttcaaaactt aaatcttaaa gtgtaaagtg taaattgcca
      781 ggattaagtt ttgcgactgg attatctgca ggaacaatat tcgtataaaa gcccgacttt
      841 ctggctgtcc tgtgaaaaac tgcaaggggc ttcctttctg tgctcaaaat atttacccgt
      901 aatttcctgc gtttcacggg aggtggccag ccaaacgggc gtatcggcac cctggtcggg
      961 cgattcatga ccgccaaatc ctaaagagtt acttaatgcc gaatttacat ccccgggatg
     1021 gcaggaattg actgtgatgc ggtatggcat cagtttgcgg gcaaatgcga ccgtaagcat
     1081 ccggttggct tgcttggatt gtcgataggc cgtatcgttt tcgtactttc tttttctgaa
     1141 ttcgggatca tccaaatcaa gcccgccggc ccagtagctg gccacattca cgatccgtgc
     1201 cggagcgcct gctttaagct gcggcgtcaa tgcgtcgatc atccggaagt agcccatgac
     1261 attgaccgca aactgcattt cgatcccttc tgaattttct atccgccttt ggggtgtgaa
     1321 tgcggcattg ttgatcaata catgcacagg cctgtttatc cgagaagcca attcgtatat
     1381 tgacactttt tcagacaggt cggcaagttc ataaacaata tcgagcgaac cggttttagc
     1441 ctggatatcc ttcacaaccc ggatggcttt ggattcatcg cgggctacca ttattacgtt
     1501 gtaattaccg gatgcggcta attgccgtac aattgcttta ccaatggctc ctgttgcacc
     1561 ggtaacaacg gctgttttct ttaattccat ctcattcgag tgaggatcat tcaaatatag
     1621 ttattttcgc ttttaaattt gttttgaaaa tatcatgata ataggcctgt ttatctattc
     1681 cctgattttt atcgccgttg gaatcctctt tgccttgcgg aagaaaaaac tgatcgcagc
     1741 catgtttttc cttctggccc tgttgcttgc cgtggttggt gtggctgcaa ttgtcgttta
     1801 tcctcatatc tggccctttt aatatgaaaa aaccagcact tatactgttg atggtattgc
     1861 tgacctgcag cgggatcacc caggtcaatc ccacttttat tggtctaaaa accggagtgt
     1921 ccgtcccggt aggacaattt ggaggtcatg atcttgttac aggcagtttc actcaggcgg
     1981 gttttcaagg cgccattgag ggggcctggt tttttaagcc ctggataggt gccgggttac
     2041 atggttttgc ggcattgcat ccgattgatg tcggaacgct tggttacgaa aaagtaatag
     2101 ccgatccgtt catggatgat gtcacgatcc ggtcagaacc tttccggact ttcggtggat
     2161 acgccggctt gtttttccat gttccgttgg ttaagaacct gtcggttacc ggaaaagccc
     2221 tgggggggat gatctatgcc aaaacgcctt accagctcta caaagccgaa tattacctgg
     2281 ttggagaaca atggtttgag ataacctcag cagccgatta tgaagccagt ttcctggccg
     2341 gtgtgggaat aaggtacgat atcaacaaaa gtaccgcatt ctgcctggac acggaattca
     2401 cctggaataa agctgatttt gagtttatta caaccggtaa cgatatccgg attgaagaaa
     2461 aggatatttc ctttctgaat atcggcgtcg gcgtgatctg gaagatttca gccggagaat
     2521 agcaaagtaa cagctttcag taatcaaaac tgactgtttc cacaggctcc agtccatacc
     2581 gaaaaaccct catcggcctt ttccccttca gctcatattt gtcgccttcc acctggatga
     2641 gagaggattc cctcagcccg atcacggtta tatcgcggtt taccgccagg aactcttcga
     2701 tccgctgctg ccgggtttca ccgccgtgtc cttccggatt ggcgtcgaga taatgcggat
     2761 tgatctggaa gggtaccagg tcgcatgtcc tgaagctatc gggttgaacg atcggcatat
     2821 cgttggttgt gcggagcgtc ggacaggcca cattggcccc ggcgctccat cccatgtaat
     2881 gcattccatc attggcgcga tcacggatca catccaccag tttcatctta tgcatcatat
     2941 ataccagata aaaggtattc ccccctccta ctgcgatggc ttctgctttt ctgacagctt
     3001 ccaccggatc ttgttcatga tgaatagaat atattccata gccgagttct ttaaaaacgg
     3061 cttgtaccct ttgctcataa atatcatagg atttttcaag cccttccggc gacaggttca
     3121 cgccggcata aggaataaaa aggatatttt tcacacctgt tcctgccagg aaggacgtaa
     3181 tataaggtct gggccaaccc aggtaaggtt cgccgggatt ggtcgaatta ctgatcaata
     3241 ataatctttt cataatataa tattaaggga ttgggaaacg aaactaatca atttttccaa
     3301 atgacaggcc agtatttcat tttccagcgg ttcttttcca gcaaggtccg tttatagctc
     3361 catttcaggt ccctgaacca tttatcgctg acctggcggg attccatggt tgtccaaggc
     3421 ttttcagaac cattcacact ataatcgcgc atcgccatta actttccaag tttatgctgc
     3481 atctccctta acggatctgc aggaatgccg agcgaataaa tatgtttcaa gacccggaaa
     3541 gagttgtcgg catctttcag ttcgatgtaa aaaaacaatg catccttaag aaaatccgga
     3601 tcggttgagg cgacgattcg ttcaatcaaa ggggcaaaag cgattccata atcgagaagt
     3661 ttattcctgg aatagtcgtt acgaagttgc tcgaaagcct ttagataatc atgccatggc
     3721 ccgttgagcc ttctctccaa gccggcctcc tgttcctggt aagcagccgg gacctccagc
     3781 cagactt
//
LOCUS       DAOQXS010000302         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25060_length_3776_cov_3.957270, whole genome shotgun sequence.
ACCESSION   DAOQXS010000302 DAOQXS010000000
VERSION     DAOQXS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25060_length_3776_cov_3.957270"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PKM34_08685"
     CDS             complement(<1..1396)
                     /locus_tag="PKM34_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83705.1"
                     /translation="MVASEKYTEDSIKSLDWREHIRLRPGMYIGKLGDGAAQDDGIYV
                     LMKEILDNCIDEHVMGFGSRIDVAIKEKTVVIRDYGRGMPLGKVIDCVSKINTGAKYD
                     SKVFKKAVGLNGVGSKAVNALSSYFKAQSVREGKTKIVEFERGDLIRDHEIAPCDLPG
                     GTEITFIPDDTIFGHYYYITEYVEKLLWNYAFLNNGLILTLNGEKFQAKNGLLDLLSL
                     YLNGNGGALYPTIHIKEEEFECAFTHSSKQYGEEYYSFVNGQYTPQGGTHLQSFREAI
                     VKTIREFYNKDYDTNDIRASIIAAISLKVQEPIFESQTKTKLGSQHMEPEGQTIRNYI
                     MDMVKKHLDNYLHKNPETAEALQLKILQSEKERKDMADIKKLAKESVKKASLHNKKLR
                     DCRVHYNTNHDDRLESTIFITEGDSASGSITKARNVSTQAVFSLKGKPLNVYGLSKKI
                     VYQNEEFNLLQSALN"
     gene            complement(1560..2165)
                     /locus_tag="PKM34_08690"
     CDS             complement(1560..2165)
                     /locus_tag="PKM34_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ83706.1"
                     /translation="MVSKKLKSPVCPDRYYHIYNRGNNKEKLFYHSGDYHFFLEKYKQ
                     YVTPYADTYAFCLLPNHFHFLIKTGEKTNASISAVSNQLRRLFISYTKRINNIHNRSG
                     SLFTKNYQRIEIDSDRYLTQVIRYIHKNPVKHNIQNDYENYHYSSYRIIVSDHETELK
                     RNEVIDWFINKKEFIDYHRNDQVDPTTFNLLSIEDENSPDG"
     gene            2379..3257
                     /locus_tag="PKM34_08695"
     CDS             2379..3257
                     /locus_tag="PKM34_08695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ83707.1"
                     /translation="MKKLFKAFRAIGLILRNPWLLNKILEDDQFWKKKINRDLGRKDV
                     LPVIGPDSLSGEFSAFIQPFAFGDGGSLPTDLSLLVKIAEMIPGCKYFEIGTWRGESV
                     ANVARVAKECYTLNLSDEEMLAMDLPLDYIAQHRVFSLGLSNVVHLQGNTKSFDFASL
                     NMKFDLIFIDGDHHFEMVKNDTQKVFQHLVHDKTIVVWHDYARSPETVRWEVLAGILA
                     GTPKEIQHHIYHVAHTLCAVYLPGFEKPDYLRPDLVKDFFQVEIKYFNETPSRKFEME
                     SHNPQTPSQPDQMESH"
     gene            3301..>3776
                     /locus_tag="PKM34_08700"
     CDS             3301..>3776
                     /locus_tag="PKM34_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83708.1"
                     /translation="MKSRIEFAQQELSKRNLYNSIIDGLLSADTLAALDALPLSYTRI
                     KPEWEALRKVYGFIQIACLEAGFDPGEIDGRWGPNTENAFNNYRYLLANGKPPAPWRP
                     EDLARINPNNWPPQYTAEFDLFYGPKGSNLVRIDLPYSMKLAWDPAVTVNSFSCHE"
BASE COUNT          946 a          833 c          863 g         1134 t
ORIGIN      
        1 cattcagcgc agattgaagc aggttgaact cttcgttctg gtagacgatc tttttagaga
       61 gcccgtagac gttgaggggt ttgcctttca ggctgaaaac ggcctgtgtg cttacattgc
      121 gggctttggt aatggaaccg ctggcgctgt cgccctctgt gatgaagatg gtactttcca
      181 gccggtcatc atggttcgta ttgtaatgga ccctgcagtc gcgcaatttc ttgttatgca
      241 ggctggcttt cttgacgctc tcttttgcca gctttttgat atcggccata tcctttcgct
      301 ctttttccga ttgcaggatc ttgagctgaa gcgcctcggc cgtttcggga ttcttgtgaa
      361 ggtaattgtc gaggtgtttt ttgaccatat ccataatata gttccggatg gtctgtccct
      421 ccggttccat atgctgggag cccagcttgg tcttggtctg cgattcgaat atcggttcct
      481 gcaccttcag gcttatggcg gcgatgatgg atgcgcggat atcgtttgta tcgtaatctt
      541 tgttgtagaa ctcgcggatc gttttgacga tggcttcgcg gaacgactgc aggtgtgtgc
      601 cgccctgcgg ggtgtactgt ccgtttacaa aggaataata ctcttcgccg tattgcttgg
      661 agctatgcgt aaaggcgcac tcgaattctt cctctttgat gtggatggta ggatagagag
      721 cgccgccgtt gccgttgagg tagaggctga gcaggtcgag cagcccgttc tttgcctgga
      781 atttttcacc gttgagggtg aggatcagtc cattattcaa aaatgcataa ttccagagca
      841 gtttttcgac atattccgtg atataataat agtgtccgaa aatagtatcg tcggggatga
      901 acgtaatttc ggtcccgccg ggcaagtcgc acggggcgat ttcatggtcg cggatcaggt
      961 cgccgcgttc aaattcaacg atcttggttt ttccttctct aacagattgg gctttgaagt
     1021 aggatgacag cgcattgaca gcttttgaac caacgccatt cagcccgact gcttttttaa
     1081 agactttgga gtcgtatttg gcgccggtat tgattttcga aacgcagtcg atcacctttc
     1141 ccagaggcat ccccctgccg taatcgcgta taacgactgt tttttccttg atcgccacat
     1201 cgatccggct tccaaagccc atgacgtgct cgtcgatgca attatcgagg atttccttca
     1261 tcaggacgta gattccgtcg tcctgtgcgg cgccatcgcc cagtttgcca atgtacatac
     1321 cgggtctgag ccggatatgt tcccgccagt cgagcgattt aatactatct tcggtatatt
     1381 tttccgaggc taccataatt cagccgcaaa tgtacaatca tgaatccttt attttacggg
     1441 cggtacatga aacttatgaa gaattttttg ttgataatcg gaattgagac tccatctgga
     1501 taaattgaga tggagtctca aagaagggag actccatctc aaaatcatga gactccatct
     1561 caaccatccg gagaattttc atcttcgatg ctcaataggt taaacgttgt agggtcaacc
     1621 tggtcattgc gatgataatc aatgaattcc ttcttgttaa taaaccaatc gatcacctca
     1681 ttccttttga gttcggtttc atgatcgctt acgattatcc tgtatgagct atagtgataa
     1741 ttctcataat cattctggat attgtgctta accggatttt tatgaatata gcgaattacc
     1801 tgggtgagat aacgatcact atcaatctcg attctttgat aattcttcgt aaaaaggctt
     1861 ccgctgcggt tatgaatatt atttattctt tttgtgtatg aaataaaaag ccttcttaat
     1921 tgattggata cggccgatat cgaagcattt gttttctcgc ctgtttttat caggaaatgg
     1981 aaatgattag gtaacaggca aaatgcatat gtatctgcat aaggtgttac atactgtttg
     2041 tacttctcca aaaagaagtg gtagtcgcct gaatggtaga ataatttctc tttattattg
     2101 cccctgttat agatgtgata gtatctgtct gggcaaaccg gtgactttaa tttcttgctt
     2161 accatggtgt taagtttttg gattaataat aatttttgag actccatctg gtttttttga
     2221 gatggagtct cacatccctc atattctatt aatccggaaa agcgatgaaa aatacccggt
     2281 cggttgaaaa tatttgagac tccatctgga taaattgaga tggagtctcg atttggtgag
     2341 ttgtaaattt ttataaattt ggtaaagaat tcatcaattt gaaaaaatta ttcaaagctt
     2401 ttcgcgccat tggattgatt ttgcgaaatc cctggcttct gaacaaaatc ctggaggatg
     2461 atcaattctg gaagaaaaaa ataaaccggg atcttgggcg aaaggatgta ttgcccgtaa
     2521 tcggtcctga ttcactttcc ggagagttct cggcatttat tcaacctttt gcattcggcg
     2581 acgggggctc gctaccgaca gacctctccc tcctggtaaa aattgccgaa atgattcccg
     2641 gctgcaaata cttcgaaatt ggcacatggc gcggcgaaag tgttgccaat gttgcacggg
     2701 tggctaagga atgctacacg ttgaacctgt cggatgaaga gatgcttgca atggatttac
     2761 ctttggatta tatcgcacaa caccgggtat tttccctggg gctttcgaat gtcgttcacc
     2821 ttcagggaaa tacaaaatca tttgattttg cgtcactgaa catgaaattt gatcttatat
     2881 ttattgatgg tgaccatcac ttcgaaatgg ttaagaatga tacacaaaaa gtgtttcaac
     2941 acctggtaca tgataaaacc atcgtggtct ggcatgatta tgcgcgcagc ccggaaacag
     3001 tcagatggga agtacttgcc gggatactgg ccggcacccc gaaagaaatc caacatcata
     3061 tttatcatgt tgctcatact ttatgtgctg tctatcttcc cggatttgaa aagccagact
     3121 atttacgacc tgatttggtt aaagattttt tccaggttga gatcaagtat tttaacgaga
     3181 ctccatctcg taaatttgag atggagtctc acaatcccca gactccatct caacccgacc
     3241 agatggagtc tcattaaatc gagatggagt ctcacttaaa catctaaaaa ccagtacccc
     3301 atgaaatcaa ggatcgaatt cgcccagcag gaactttcga aacgaaatct ttacaactcc
     3361 ataattgacg gattactaag tgccgatacg ctggctgccc tcgatgcttt gcctttgtca
     3421 tatacccgga tcaaaccgga atgggaagcg cttcgcaaag tttacggatt tatccagata
     3481 gcctgcctgg aagccggctt cgatccggga gagatcgacg gccgctgggg gcccaacacc
     3541 gagaatgcat ttaacaacta ccgctaccta ctggccaacg gtaaaccgcc agctccatgg
     3601 cggccggaag atttggcccg gatcaacccc aataactggc caccccagta cactgctgag
     3661 ttcgatttgt tctacggccc caaaggctcc aaccttgtca ggatagacct tccttacagc
     3721 atgaaactgg cctgggatcc cgctgtaacg gttaattcat tctcctgcca cgaaaa
//
LOCUS       DAOQXS010000303         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25094_length_3773_cov_3.282141, whole genome shotgun sequence.
ACCESSION   DAOQXS010000303 DAOQXS010000000
VERSION     DAOQXS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25094_length_3773_cov_3.282141"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>813
                     /gene="sprA"
                     /locus_tag="PKM34_08705"
     CDS             <1..>813
                     /gene="sprA"
                     /locus_tag="PKM34_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNQ83709.1"
                     /translation="TDVDTTIWGRVPTLQNLVESFDNNPASRPFQDVGYDGLRNEDER
                     DFYGISYIDRIEQVHGTASIAYFSALEDPSADDYNYFRDSDYDNDDKYSTILERYKKY
                     NGPDGNSPTDSQNDEEYPTSATAIPNVEDLNRDNTLSEAERYFQYVIDLDPNKMEVGE
                     NFITDIRTAKGVPLANGTTGEVNWYQFKVPVAQPSRVVGNISDFKSIRFLRMFMKDFE
                     KPVTLRFATLELVRGEWRRYKGDLLSAGEYIPDDIQSLTTFDIFSVNIEENGR"
     assembly_gap    814..823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <824..>3773
                     /gene="sprA"
                     /locus_tag="PKM34_08710"
     CDS             <824..>3773
                     /gene="sprA"
                     /locus_tag="PKM34_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNQ83710.1"
                     /translation="QPVPYVLPPGLEREQNIGTTTLVRLNEQSMVLKVCDLIDGDSRA
                     AYKTTDFDFRQYKNLEMFVHAEKSRNEDVVNDGDLTVFVRIGSDFTENYYEYEVPMTF
                     TPWGTPPSQADVIWPESNRFNIDLEKLVQVKLDRDIAARNGESNVSVNFPYVRQEGPN
                     RITVLGVPSVSDVMAIMVGVRNPKRQSLTGEDDGLSKCAEIWINELRLTGFNDKAGWA
                     GLIRVNSQLGDIGQVVFSTMYSSPNFASLEKKINDTQKESHLQWDIATDLNLGKFLPE
                     EAGVRIPMHIDYSQTIETPEYDPLSPDLKLKDELASYENKEEADSIRDIVQDYTDRFN
                     INFMNVRKDRVGAKKKPQFYDVENFSVSYAYTMLNMRNIDIEYDKKETHNGGLAYNFN
                     IAPKNIKPFQNIGFISKSEFLKLFKDFNFYFLPKQFGFRTDMMREEAERKLRNKSEGL
                     ILLRPTFARKWDWNRMYDLKFDLATSLTLQYRASVNSFLREPSGSLDPNSPWYDKAGA
                     DTIDVGRQFWQGGLRRNYNQTLDITYRIPIDKLPMMDWVTAQATYGTIYNWIASPLSV
                     QQRLGNVIENSRNIQLSGNVDLTKLYGKVPYLKKLGQSAKPDTRGRSGTVPKQQPPPQ
                     EEQDSVAKPNYFKKVADEFLKFVMMVKRGSITYSESTGTLIPGFMPSPQALGVDWGLN
                     APGLGFAFGSQKDIRSQAVQERWLTYDTLQNQAYANKFTSSLNARVSLEPFQGFKIEI
                     NADRTFARNHTEYFRADSSGVFDSYSPQDAGSFSMSYITWGTAFHTDYDTSISATFEE
                     MKRNRYDIAMRLAAENPNSNAMVYDSLSQQYYPLGYGPTSQDVLIPAFLAAYGNKSTT
                     EVYLGYFPSIPLPNWRVTYDGLTKIKFLQKFIKSAVLSHSYRSVYTISSYQSNLYYGE
                     TDGKPSALYEASNSYYPKYDLNQVTIQEQFAPVVGIDITLNNSLLTRFEFRKARTLTF
                     SMINKQLTDLS"
BASE COUNT         1028 a          936 c          958 g          841 t
ORIGIN      
        1 gaccgatgtc gataccacca tctggggaag ggtgcctacc ctccaaaacc tggtcgaatc
       61 gttcgacaac aacccggctt cccggccatt ccaggatgtg ggttatgacg gcttgcgtaa
      121 cgaagatgaa agggacttct atggaatttc atacatcgac cggatcgagc aggtccatgg
      181 tacggcatcg atagcctact ttagcgcatt ggaagatccg tcagccgacg attataatta
      241 tttcagggat tcggattatg ataatgatga taaatattcc actatccttg aaaggtacaa
      301 aaagtataac gggcccgacg gcaacagccc tacggattcg cagaacgatg aggaataccc
      361 gacatcggcc acggcgatcc ccaatgtgga agacctgaac cgcgacaata cactcagcga
      421 agcggaacgc tatttccagt atgtgatcga tcttgacccg aataaaatgg aggtcggtga
      481 gaattttatc accgacatcc gcacggcaaa aggagtgccg ctggccaacg gtacgaccgg
      541 tgaggttaac tggtaccagt tcaaggtacc ggtcgcccag cccagccggg tggtgggtaa
      601 tatcagcgat ttcaaatcga tccgcttcct caggatgttc atgaaggatt tcgaaaaacc
      661 ggtcacatta aggtttgcca ccctggagct ggtcaggggc gaatggcggc gctataaagg
      721 cgatttgctt tcggccggcg aatatatccc cgatgatatc cagagcctga ccactttcga
      781 tattttcagt gtaaatattg aagagaacgg acgnnnnnnn nnncagccgg tcccgtatgt
      841 cctgcccccc gggcttgaac gggagcagaa tattggcacc actaccctgg tccggctgaa
      901 tgaacaatcg atggtactga aagtttgtga cctgatcgac ggcgattccc gggcagctta
      961 caaaaccacc gatttcgact tccggcagta taaaaacctg gaaatgttcg tgcatgccga
     1021 gaaatccagg aatgaagatg ttgtcaatga tggcgatctg acggtttttg tcaggatcgg
     1081 ttccgatttt accgagaact actacgaata tgaggtgccg atgaccttta caccctgggg
     1141 aacgcctccc tctcaggcgg atgtcatctg gcccgaatcg aacaggttta atatcgacct
     1201 tgagaaactc gtgcaggtaa aactcgaccg ggatattgca gccagaaacg gggaaagcaa
     1261 tgtgtcggtc aattttccct acgtcaggca agaagggcca aacaggatca cagtgctcgg
     1321 cgttccttcg gtcagtgatg tcatggcgat catggtcgga gtgaggaatc ccaaacggca
     1381 atcgctgacc ggtgaagacg acgggctgtc gaaatgtgcc gagatctgga tcaatgagct
     1441 caggctgacg gggtttaatg ataaagccgg gtgggcagga ctgatccgcg ttaattccca
     1501 gctcggcgat ataggccagg tggtcttttc aaccatgtac agttcaccca attttgcttc
     1561 ccttgagaag aaaatcaacg atactcaaaa ggaatcgcac ctgcaatggg atatcgccac
     1621 cgacctgaac ctcgggaaat tcctccccga ggaggccggc gtgagaatcc ccatgcatat
     1681 cgactattcg cagacgatcg aaaccccgga atacgaccct ctcagccccg acctgaaact
     1741 gaaagatgaa ctggcatcct atgaaaacaa agaggaagcg gattcgatac gggatattgt
     1801 ccaggattat accgaccggt tcaatatcaa tttcatgaat gtccgcaagg atagggtagg
     1861 ggctaaaaag aaaccacaat tctatgatgt ggagaatttc agcgtaagtt atgcttatac
     1921 catgctgaat atgaggaata tcgacatcga atacgataaa aaggaaacgc ataacggggg
     1981 acttgcctat aatttcaata ttgcaccgaa aaatatcaag ccatttcaga atataggttt
     2041 tatctccaaa tcggagtttc tcaagttgtt taaagacttt aatttctatt ttctgcccaa
     2101 gcagtttgga ttccggaccg atatgatgag ggaagaagcg gaacgaaaac tcaggaataa
     2161 gagcgaagga ttgatattat tgcgtccgac ttttgcgagg aagtgggact ggaaccggat
     2221 gtacgacctg aagtttgacc tggcgaccag cctgacgctg cagtaccggg cctcagtgaa
     2281 ctcgtttctc cgggagccgt ccggctcgct ggatcccaat tctccctggt acgacaaagc
     2341 cggcgccgat acaattgatg tcggcagaca gttctggcag ggaggactgc gccggaatta
     2401 caaccagacc ctcgatatca cctaccggat tccgatcgat aagcttccga tgatggactg
     2461 ggttacggca caggctacct acggaacgat ctataattgg attgcgtcgc cgctttcagt
     2521 gcagcagagg cttggcaatg tgatcgaaaa ttcaaggaac atccagttat ccgggaacgt
     2581 cgacctgacc aaactttatg gaaaagtgcc ctacctgaaa aaactgggcc agtcggccaa
     2641 acccgataca cgcggacggt cggggaccgt gccgaaacaa cagccgcctc cccaggagga
     2701 acaggacagt gtggccaaac ccaactattt caagaaagtg gcggatgaat ttctcaaatt
     2761 tgtcatgatg gtcaagcggg gaagcattac ctattcggaa agcacaggaa cactgatccc
     2821 cggttttatg ccatcgccgc aggcgctggg agtcgactgg gggctgaatg cgccgggcct
     2881 tggatttgcc ttcggaagcc agaaagatat ccggtcgcag gctgtgcagg aaaggtggct
     2941 cacctacgat accctgcaaa accaggcgta tgccaataaa ttcacctcca gcctgaacgc
     3001 ccgtgtttca ctggagccat tccagggctt taagattgag ataaatgccg accggacctt
     3061 tgcccgcaac catacagagt atttcagggc cgattccagc ggggtctttg atagctattc
     3121 ccctcaggat gccggtagtt tcagcatgtc gtacatcacc tggggaacgg cattccacac
     3181 cgattacgat acctcgatct cagcgacatt tgaagaaatg aagagaaacc ggtatgatat
     3241 cgccatgagg ctggccgctg agaatccgaa ttcaaatgcc atggtctacg attccctttc
     3301 tcagcagtat tatccgctgg gctatgggcc gacgtcgcag gacgtgctca tcccggcatt
     3361 tttagccgct tacggaaata aaagcacgac ggaagtttac ctgggttatt tcccgagcat
     3421 ccccctcccg aactggagag tcacttacga tggcctgacc aagattaaat tcctgcaaaa
     3481 gttcataaaa tcggctgttt tatcacattc ctaccgatcg gtttatacca taagttccta
     3541 tcagtcaaac ctgtattatg gagagactga cgggaaaccg tcggcattgt atgaggcatc
     3601 caattcctac tatccaaaat atgacctgaa ccaggtgacg atccaggaac aatttgcacc
     3661 agtcgtagga atcgatataa cgctgaataa cagcctccta acacggttcg agttccggaa
     3721 ggcccgcacc ctgactttca gcatgatcaa taagcagctc accgatctga gta
//
LOCUS       DAOQXS010000304         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25181_length_3765_cov_4.258491, whole genome shotgun sequence.
ACCESSION   DAOQXS010000304 DAOQXS010000000
VERSION     DAOQXS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25181_length_3765_cov_4.258491"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKM34_08715"
     CDS             complement(<1..200)
                     /locus_tag="PKM34_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83711.1"
                     /translation="MTGYFKRVIFILLLIFAAGCGPNPKKGQIPETRIQKIKVKNYGQ
                     ALFSLDPMNVKQGLDSLSSEFS"
     gene            complement(234..830)
                     /locus_tag="PKM34_08720"
     CDS             complement(234..830)
                     /locus_tag="PKM34_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNQ83712.1"
                     /translation="MKKIIVIIFLLFIAAENYGQFTFGPKIGYNAAKLSTDFDTIKES
                     VRSTFQIGAFARLGKKLYVQPELYYATSGGTFRIEGTTLKQDIRMNNICVPVLIGYKV
                     INAKVINLRVMAGPVANFIINKKLETSDLIEYPLQDSDLKNIAWGLDLGAGADIFFLS
                     LDIRYEFGLNNIYIIPENATDNKIKSNMFIVSLGFKLI"
     gene            935..1630
                     /locus_tag="PKM34_08725"
     CDS             935..1630
                     /locus_tag="PKM34_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNQ83713.1"
                     /translation="MKKIFIFILFLFISAQAISQYKTVLFGLRAGANLAWLNPDTEGY
                     DSDGVKPGFSWGFTGEFYMMENYAITTGFNMNFTGGKMNYPYSLHIPGTDSTILSPGV
                     MNRDYSFKYLQIPLCLKMQTELSERVTIFGKIGLGTAFNLDAKATGHFEADDGQIHED
                     KKNIGDDIALMRESLLIGGGVEITIKGSTAVIIDFMYDNAFNNILSGDNPFVPDKPRA
                     VQNFVELGAGIVF"
     gene            1643..3286
                     /locus_tag="PKM34_08730"
     CDS             1643..3286
                     /locus_tag="PKM34_08730"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNQ83714.1"
                     /translation="MKIALAQLNYHIGNFDENVGKIKGAIRKAKALKADLAVFSELAV
                     SGYPPRDFLEFGDFIDRCEKAIGEIADECRGIAAVIGCPVRNPDVKGKNLLNAALFLE
                     EGQVRHAYYKALLPNYDVFDEYRYFEPGRKFGTVLFKGKKLALTVCEDLWDIGAEDPL
                     YVVNPMEEMILRNPDLIINIAASPFHYKQSEIRKGILTENAKKYHLPVFYVNHVGAQT
                     ELLFDGGSMVVGPDAQNLAELKYFEEDFAVFDLDEVISRKETVAGEEKSPVAWIHDAL
                     VMGIRDYFSKLGFSKAILGLSGGIDSAVTLALAAEALGKENVWAVLLPSAFSSDHSVS
                     DAIKLADNLDCRYDIIPITPSYDTLGKTLNPWFEGLPFGVAEENLQARTRGVILMALS
                     NKFGYILLNTSNKSEAAVGYGTLYGDMNGGISVLGDVYKTQVFEMAHFINRHQEIIPL
                     NTIMKPPSAELRHNQKDSDSLPEYDILDKILFQYIEMRLGPKEIIASGFDEAVVKRVL
                     WMVNTNEYKRHQTPPILRVSSKAFGMGRRMPIVARYLAV"
     gene            complement(3287..3535)
                     /locus_tag="PKM34_08735"
     CDS             complement(3287..3535)
                     /locus_tag="PKM34_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNQ83715.1"
                     /translation="MEENRNELEKKVQNVLEQIRPYLQSDGGDIKYINLTDDNIVNVE
                     LMGACGSCPYSLMTLKNGVETAIKKAIPAIKAVEAVNI"
     gene            complement(3553..>3765)
                     /locus_tag="PKM34_08740"
     CDS             complement(3553..>3765)
                     /locus_tag="PKM34_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNQ83716.1"
                     /translation="ENKYYLFGKHGGRRLADDERVNLLAEIPLVQSICESGDTGSPVA
                     LDEQSPVYKAFMNLAINIRAIIEHNN"
BASE COUNT         1009 a          788 c          891 g         1077 t
ORIGIN      
        1 atgctgaatt ccgacgatag tgaatccagc ccttgtttta cattcatggg gtcgagcgaa
       61 aacaaagcct ggccataatt tttaactttg attttttgaa tgcgggtctc tggtatctgg
      121 cctttttttg gattcggtcc acatcccgcc gcaaaaatca gcagcaggat aaaaattact
      181 ctcttgaaat agcctgtcat gcaataaaaa cgccggttaa aacctgttta ttgtcagatc
      241 agtttgaatc ccaggctgac aatgaacata ttacttttga tcttattgtc ggttgcgttt
      301 tcaggaatga tgtaaatgtt gttgagaccg aactcgtaac gaatgtcaag ggaaaggaag
      361 aagatatcgg cacctgcacc caggtcaagg ccccatgcta tatttttcag gtcgctatcc
      421 tgtagaggat attcaataag gtcgctggtt tcaagttttt tattaataat gaagttggca
      481 accggacctg ccatcacccg aaggttgatc acttttgcat tgatcacctt ataacctatc
      541 agcaccggga cacaaatatt attcatccgg atatcctgct tgagtgttgt accttctatt
      601 ctgaaggttc ctccgctagt ggcatagtac agttcgggct gcacgtagag tttctttccc
      661 agcctggcaa aggcgccgat ctgaaaagtg ctcctgacac tttccttgat cgtgtcgaaa
      721 tcagtggata atttggctgc attgtaaccg atcttcgggc caaaagtaaa ctggccgtaa
      781 ttttctgctg cgataaaaag caggaaaata atgacgataa tttttttcat aatagagcta
      841 tttggcaagc aaatgtaaaa agaaattcat tcattatacc aataaagcga tattacgatt
      901 ttaggattac ttttgttgga aaatcgatag tttcatgaaa aagatcttca tttttatcct
      961 gttcctattc atatcagccc aggctatttc ccagtataaa acggttttat tcggtttgcg
     1021 ggctggggcg aacctcgcat ggttaaatcc tgatacggaa ggttatgatt ccgacggcgt
     1081 taaaccgggt ttttcatggg gatttaccgg tgaattttac atgatggaaa actatgccat
     1141 tacaacgggt ttcaatatga acttcacggg cggaaaaatg aattatcctt acagtttgca
     1201 tattcccggt actgatagta ccatcctttc accaggggtt atgaacagag attacagctt
     1261 caaatacctt caaattcctt tatgtctaaa aatgcagact gagttatcgg aaagggtaac
     1321 gatcttcggg aaaattggcc tgggaactgc ttttaacctc gatgctaaag ccacgggtca
     1381 ttttgaagca gatgacgggc agattcatga ggacaagaaa aatatcggcg atgatatagc
     1441 tttgatgcgt gaatcacttc tgatcggagg aggggtggag atcaccatca aaggctcaac
     1501 agctgttatt attgatttta tgtacgataa tgcatttaat aatatactgt cgggtgacaa
     1561 tcctttcgtt ccggacaaac ccagagcggt acagaatttt gtggaactgg gcgccggtat
     1621 cgtcttttaa ttgtgggcaa aaatgaaaat tgcactggca cagttgaatt accacattgg
     1681 taattttgat gaaaatgttg gcaagatcaa aggtgcgatc cggaaagcca aagcattgaa
     1741 ggccgatttg gcagtcttct ccgaactcgc cgtatcgggt tatccgccac gggattttct
     1801 tgaattcggc gatttcattg acagatgcga aaaagcgata ggcgaaattg ctgatgaatg
     1861 caggggcatt gcagctgtta ttggttgccc ggtgagaaac ccggatgtta aaggtaagaa
     1921 tttgcttaat gccgctttgt tcctggagga ggggcaggtt cggcatgcat attataaggc
     1981 cctgttgccg aattacgatg tgttcgatga ataccggtat ttcgaaccgg gaagaaaatt
     2041 cgggacggtg ctgttcaaag gtaagaaact cgccctgaca gtatgtgaag acctttggga
     2101 tatcggagca gaagatcctt tgtatgtggt caaccctatg gaagagatga tcctccggaa
     2161 tcccgatttg attatcaaca ttgccgcttc gcctttccat tataaacagt cggagatcag
     2221 aaaaggtatc ctgactgaga atgcgaaaaa ataccatttg cctgtctttt atgtcaatca
     2281 cgtgggagcc cagaccgaac tgcttttcga tggcggatcc atggtggttg gccctgacgc
     2341 acaaaacctg gcagagctta agtattttga agaagatttt gctgtttttg acctggacga
     2401 ggtcataagc cgcaaggaaa cggttgccgg tgaggaaaaa agcccggtag cctggattca
     2461 tgacgcactg gtaatgggga tcagggacta tttctccaaa cttggattca gcaaagcaat
     2521 tctgggatta tcgggaggga tagactcggc tgtgacgctg gcccttgccg ccgaggcgct
     2581 gggaaaagag aacgtttggg ctgtcctgtt accatcggca ttctcttccg atcattctgt
     2641 aagcgatgcg ataaaactgg cggataacct cgattgccgg tacgatatca tcccgatcac
     2701 gccttcgtac gatacactgg gaaaaacatt gaatccctgg tttgaaggat taccctttgg
     2761 tgttgctgag gagaatctgc aggcacgtac caggggtgtg atacttatgg cactatccaa
     2821 taaattcggg tatattttac tcaatacttc caataagagc gaagcggcag tgggttacgg
     2881 tactctttac ggggatatga acgggggaat ttctgtgctg ggcgacgttt ataagaccca
     2941 ggtatttgaa atggctcatt tcattaaccg gcatcaggaa ataatccccc tgaataccat
     3001 catgaagcct ccttcggccg aattgaggca caaccagaag gacagcgatt cgctgcctga
     3061 atatgatatc ctggataaaa ttctttttca gtatatagaa atgaggctgg ggccaaaaga
     3121 aatcatcgca tcaggatttg atgaagcggt tgtaaagcgt gttctctgga tggtcaacac
     3181 gaatgaatac aaacgtcatc aaaccccacc gatactaagg gtttcctcca aagcatttgg
     3241 tatgggcagg cgtatgccga tagtggcccg ctatttggct gtctgatcag atattcacgg
     3301 cttccacggc ttttatcgcc gggatcgctt ttttgattgc cgtttccacg ccgtttttca
     3361 atgtcatcag gctgtacggg catgaaccgc atgcccccat cagttcgaca ttcacaatat
     3421 tatcgtccgt cagattgata tatttgatgt ctcccccgtc cgattgcagg taaggccgga
     3481 tttgctccag tacattctga actttctttt ccagttcgtt acgattttct tccattatat
     3541 tagagttgtt gcttagttat tatgttcaat gatagcccgg atgttgatcg ccaggttcat
     3601 aaatgcttta taaacaggag attgttcatc gagtgccaca gggctgccgg tatctcccga
     3661 ttcgcagatg ctttgcacca aagggatttc agccagcaaa ttgacccgct catcatccgc
     3721 aagcctcctg cctccgtgct taccgaatag gtaatacttg ttttc
//
LOCUS       DAOQXS010000305         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25233_length_3760_cov_3.119028, whole genome shotgun sequence.
ACCESSION   DAOQXS010000305 DAOQXS010000000
VERSION     DAOQXS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25233_length_3760_cov_3.119028"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..844)
                     /gene="mnmD"
                     /locus_tag="PKM34_08745"
     CDS             complement(179..844)
                     /gene="mnmD"
                     /locus_tag="PKM34_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNQ83717.1"
                     /translation="MEIIRTADGSSSLFVPEMGEHYHSVNGAITESIHIFINAGLKYV
                     MNDQNPVRILEIGFGTGLNAFLTWHEARAAKINVHYTAIEKYPLKQEVYHQLNYPELM
                     GYPFGEECFMKIHELHWDAPHEVMEHFILHKINTDIEDYYPGKDRFDLVYFDAFGPGF
                     QPDMWTTDLFKKMATALRPGGILVTYSTKGTVKRSVKEAGFRIEKLPGPIGKREILRA
                     LVI"
     gene            complement(871..2148)
                     /locus_tag="PKM34_08750"
     CDS             complement(871..2148)
                     /locus_tag="PKM34_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83718.1"
                     /translation="MMRKSLVAIILLGVVLFSCKKEQDTLPPVISFIAPGVNTIINIP
                     DTLDVKVQVNDDRIIHFVVISIVNEDKIPLTEAKSFAPFLAEFTAVHSFVIDSKTIAS
                     GVYSIQVLAFDGFNTKTGFLDIEINEIPRSLEGFMAVTAPLSFQSEIFRLGQDFSIDS
                     QMSFPQGCQFTAVHPVWERFLFAGGDPSVLTAINPFTFVTEWELPATPPRPQFTAIEA
                     GKDILFATANGDAGIVDIDGNVTLRTFPYNNMKILCLAADENYMYAAQQSTGGTNNEL
                     TLFYRETGAVKSRKLVAERISGIVALDDQALVFFSPGNRTEIFHYNPESFILNKLNEI
                     EGEILVSAEKVSDDQIIICTDAAIRLFRPGINRLDMLLEQPYRFCRYDALGNRLFLAK
                     DYEMEVRNFTTLDLTEEISFNDMIVDFRILYNK"
     gene            2236..2946
                     /locus_tag="PKM34_08755"
     CDS             2236..2946
                     /locus_tag="PKM34_08755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ83719.1"
                     /translation="MLKDIAIENVLFLDIETAPAFPEYSKAEERLKKLWDKKSEQLKR
                     EPDDTPESLFPRAGIYAEFGKVICISAGLFKGREFRIKSFYGDDEYQLLVQFCKMLNS
                     HYGGEKKMLCAHNGKEFDFPFLARRILIHGIPLPAILDVAGRKPWEVQFLDTMELWKF
                     GDYKHYTSLELLAAIFGIPTPKDDISGSDIHRVYWEDHDLERIRTYCQKDVLTVARLF
                     LKYQQQPVIEDDDVILVE"
     gene            3093..3758
                     /locus_tag="PKM34_08760"
     CDS             3093..3758
                     /locus_tag="PKM34_08760"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNQ83720.1"
                     /translation="MMPITKPTLIVNKEQCLANISAMAEKARMTETQFRPHFKTHNSA
                     HIGNWFRKMGIHKITVSSVSMAKFFSAYGWRDITVAFPVNLLELGQISELAAELTLNI
                     IGDTSAILPALENGLTYRAGFFIEIDTGHHRTGVFWKDLAEIDRMLEFLSGSNRLQFK
                     GFLTHAGHTYSADSHEEIIDIYKDSVRKMAYLKDFYKEAWPELIISIGDTPGCIRAYP
                     RSK"
BASE COUNT         1010 a          894 c          959 g          897 t
ORIGIN      
        1 ggagaagaag atatgctgga aggatttttg agctggtgcc gccgcgggcc gcaatgggcg
       61 agagtggagg gattcgaggt gcaggagcag ccggtgatgg ggtttgaggg gtttaaggtg
      121 agataggcga attattcacc acagattaca cagagttacg cagaggagca ctgagatatc
      181 aaattaccaa tgcccgtaga atctcgcgtt tgccaatagg tcccggcaac ttctcaattc
      241 taaaccccgc ctctttcaca cttcttttca ccgttccttt tgtcgaatac gtgaccagta
      301 tcccgcccgg cctcaatgca gttgccatct tcttaaaaag atctgtagtc cacatgtccg
      361 gttgaaaacc ggggccgaat gcatcgaaat aaaccaggtc gaaccggtcc ttacccggat
      421 agtaatcctc gatgtccgta tttattttat gaagaatgaa gtgttccata acctcgtgcg
      481 gtgcatccca gtgcagttcg tgaatcttca taaaacactc ttctccaaaa ggataaccca
      541 ttaattccgg ataattcagt tgatgataaa cctcctgttt taaaggatac ttttcaatgg
      601 ctgtgtaatg cacattaatt ttcgcggccc gggcctcgtg ccaggtcagg aaagcattca
      661 gcccggtgcc aaacccgatc tccaggatcc tgaccggatt ctgatcattc atgacatatt
      721 tcaggccggc attgataaag atatggatcg attcggtgat ggccccgttt acgctgtgat
      781 aatgttcgcc catttccgga acaaaaagag aggacgaacc gtcggctgtc cggatgatct
      841 ccacttccgg ggatttcgtg tcgttttcca tcatttatta taaagaatcc tgaaatccac
      901 gatcatatca ttaaaagaaa tctcctccgt caaatccagt gtggtgaaat tcctcacctc
      961 catctcatag tccttagcca ggaatagccg gttacccagt gcatcgtagc gacaaaaccg
     1021 gtaaggctgc tccaggagca tgtcgagccg gttgatgcct ggacgaaaga ggcggatagc
     1081 ggcatcggta caaataataa tctgatcatc agaaactttc tcggcagaca ccaggatctc
     1141 gccctcaatt tcattcaatt tattcaggat aaagctctcc gggttataat ggaagatctc
     1201 ggtccggttg ccgggcgaaa agaaaaccag cgcctgatcg tccaaagcta caattccgga
     1261 tattctttcg gcaacaagtt tccgggactt caccgcaccg gtttccctgt aaaacagggt
     1321 gagttcgttg ttggtcccgc cggtcgactg ctgggcggca tacatataat tttcatctgc
     1381 cgcaaggcag agtatcttca tgttgttgta cggaaatgtc cggagggtca catttccgtc
     1441 gatatccacg atccctgcgt cgccgttggc tgtagcgaac agaatgtctt taccggcctc
     1501 gatggcagtg aactgaggcc tgggcggcgt ggcgggaagc tcccattcgg tcacaaaggt
     1561 aaaggggtta atggctgtca ataccgaagg atctcctccg gcaaagagga accgttccca
     1621 gacgggatga actgctgtaa actggcaccc ctgagggaaa ctcatttgcg aatcgatgct
     1681 gaaatcctgg cccagccgga agatctccga ttggaagctg agtggagcgg ttacggccat
     1741 gaaaccctct aatgacctgg ggatttcatt gatctcgata tccaggaagc cggttttggt
     1801 attgaatccg tcgaaagcca aaacctggat tgaataaaca cccgatgcga ttgttttgga
     1861 atcgatcaca aacgaatgaa cggctgtaaa ctccgccaga aaaggggcaa aagatttggc
     1921 ttccgtaagg ggtatcttgt cttcattcac gatgcttatt accacaaaat ggatgatacg
     1981 gtcgtcgtta acctgcactt tcacatcgag agtatcggga atattgataa tggtgttcac
     2041 acccggggcg ataaaactga tgaccggagg cagtgtatcc tgttcctttt tacaggaaaa
     2101 taaaacaaca ccaagcagga tgatcgcaac taaagatttt ctcatcatct tacaaaaata
     2161 atgcggataa ctgaaaaatg aagctcaggg aatggcatcc cgtatgtttt tataatttta
     2221 gcaaaaattg aatttatgtt gaaagatatt gccatagaga acgtattgtt cctggacatt
     2281 gagacggctc cggcttttcc ggaatacagc aaggcggaag aaaggctgaa gaagttatgg
     2341 gacaagaaga gcgagcagtt gaagcgggaa ccggacgaca cgccggaaag tctattcccg
     2401 agagcaggca tttatgctga gttcggtaag gtgatctgta tttcggcggg tttgttcaag
     2461 ggcagggagt ttcggataaa atctttttat ggcgacgacg agtaccagct actggtgcag
     2521 ttctgtaaaa tgctgaattc gcattacggc ggggagaaga agatgctttg cgcacacaac
     2581 ggcaaggagt ttgattttcc gttcctggcg cggcgcatcc tgatccacgg aataccgttg
     2641 ccggccatcc tcgatgtggc gggacgaaag ccctgggaag tgcaattcct ggatacgatg
     2701 gagttatgga agttcggcga ctacaagcat tacacctccc tggaattgct ggctgccatt
     2761 ttcggtatac ccacaccaaa ggacgatatc agcggaagcg atattcaccg ggtttactgg
     2821 gaggatcatg acctggagcg gatcaggacc tattgccaga aggatgtcct gacggtcgcc
     2881 cggcttttcc tgaaatacca gcagcaaccc gtgatcgaag atgatgatgt gatcctggtg
     2941 gaataacggg tagcggtcta ttatgcccaa acccctacac ttcagaatac agaaaaatac
     3001 atacatttgt tttaccaacg gacaacggac aacggacaac ggacaacgga caatggacaa
     3061 cggacaatgg acaacggaca atccttactt gaatgatgcc gataacaaaa ccgactttaa
     3121 ttgtcaataa agagcagtgc ctggccaaca tcagcgcgat ggcggagaag gcccggatga
     3181 cggaaacgca attcaggccg catttcaaaa cgcacaattc ggcgcatatt ggcaactggt
     3241 tccggaaaat gggcattcac aagatcacgg tttcatcggt cagcatggcc aaatttttct
     3301 ccgcatacgg ctggcgcgac atcacggtag cgttcccggt caacctgctg gaactcggcc
     3361 agatcagcga actggcggct gagttgaccc tgaacattat cggcgacaca tcggctatcc
     3421 ttccggcatt ggagaacggg ctcacatacc gggccgggtt tttcattgaa atcgacaccg
     3481 ggcaccaccg gacgggtgtt ttctggaaag acctggcgga gattgaccgg atgctggaat
     3541 ttttatcggg ctcgaaccgg ctgcaattca aaggattttt aacccatgcc ggccatacct
     3601 actcggccga cagtcatgaa gagatcatcg atatttacaa agacagcgtc aggaagatgg
     3661 cctacctcaa agatttctac aaggaggcct ggcctgaact gatcatttcg atcggggata
     3721 cgccaggctg catccgggcg tatccccgat cgaaatgatc
//
LOCUS       DAOQXS010000306         3759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25243_length_3759_cov_2.953834, whole genome shotgun sequence.
ACCESSION   DAOQXS010000306 DAOQXS010000000
VERSION     DAOQXS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25243_length_3759_cov_2.953834"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1151
                     /locus_tag="PKM34_08765"
     CDS             240..1151
                     /locus_tag="PKM34_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83721.1"
                     /translation="MKKILIIYYTQTGQLRQIVDSITEPLKNHCLLFFEELRPVPSYP
                     FPWNGMPFFQVFPESVREIPCELEPLANNPDLDYNLVILAYQVWYLSPSIPMTSFLKS
                     EAAGKILKGKPVITVLGVRNMWIMAQERVKAMIAGAGGKYCGNIVMADPAGNLTSVIT
                     IVRWMMKGEKTPFRWMGVNFPAAGVPYEEIGRGREYGRLIMHYLISSFTGELQEDLVR
                     QGAVKVNPVLYKIEKTGKRIFGIWSKLVLAKGGYNDPAREGRLKAFQYYLFAVIYLVS
                     PIVAAVNRFLFFLNPGAARKIVDRFSR"
     assembly_gap    1419..1518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1521..2606
                     /locus_tag="PKM34_08770"
     CDS             1521..2606
                     /locus_tag="PKM34_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNQ83722.1"
                     /translation="MDNDRMEDFLGMIDGKPSKAKRIILRNNQITQRYYALDEKGKLT
                     HNNAQLTAAAIRLLFDKDFTAEDIELLACGTTSPDQTLPSHASMVHGELGGKPLEIIS
                     PSGSCATGMHAMKYAYLAVKAGEKNNAVCSGSELFSIMMQADKFEKETENLHLLDQNP
                     ILAFEKEFLRWMLSDGAGAALLQNSPNPDGLSLRIDWIESASFAGRLETCMYSGGEKM
                     PDGNVKGWKEFDSQDILNKSLFSLHQDVKLLGENIYQTCNMFLADIVKRRNLDLNQID
                     YFLPHISSEYFRYRIDADSIEKGVHIPQEKWFTNLVRLGNVGAASIYFMLEEIFHSGT
                     LKKGQKLLLGVPESARFSYVYSLVTVV"
     gene            2652..3092
                     /locus_tag="PKM34_08775"
     CDS             2652..3092
                     /locus_tag="PKM34_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83723.1"
                     /translation="MLVPKEKIMELLPQRPPMVMVDELVMCNEMEAVSRLFIRDDNIF
                     LGETGFSPSGMLEAMAQTAAARTGYLLKKQPESAHKSIPVGVIGSIKNFKLHFQPGSG
                     DTIFTTVFVEHEVLQATVIRAKVEAEGKLAAESNLQIFLSEEQT"
     gene            3095..3520
                     /locus_tag="PKM34_08780"
     CDS             3095..3520
                     /locus_tag="PKM34_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83724.1"
                     /translation="MKKKEVPQDEGLSDGLFEDICYALDDDGHYVVVPTTGFQPKTDA
                     LLQAWDVIHEKVEAVRQQVLAGNLSPIAYFMEKNIMDARLLADYTELPKWKVRRHLKP
                     KYFNRLDNSILDRYADTFGISREQLMDIRTIQEDKHEPG"
     gene            3507..>3759
                     /locus_tag="PKM34_08785"
     CDS             3507..>3759
                     /locus_tag="PKM34_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNQ83725.1"
                     /translation="MSQDKLLIDFQHKPAAHCENGATSNLLNHYNIRLSEPMVFGIGS
                     GLFFSYMPFLKLNGIPVVSFRPWPGTIFNRNTRRLGIEIQ"
BASE COUNT          983 a          887 c          891 g          898 t
ORIGIN      
        1 gcaaaagaat ttcgcaggag gatatcgaac cggtactgca ggattatgtt tctgcactgg
       61 agacgatgct gaagaagtac ccggagcagt ggttcaatta ctacgatttc tggattgatt
      121 aatttgtgaa aattcgttgc atccgtgatt attcgcgttc tcttattaga acacggatga
      181 tgcgaatttt acgaatcttc gcggatatat ttaattttga atcccatttc ggttagaaaa
      241 tgaagaaaat actgataatt tactataccc aaactggcca gttaaggcaa atcgtcgact
      301 cgatcactga accgttgaag aaccactgcc tgcttttttt cgaagaactc aggcccgtcc
      361 cctcctatcc tttcccctgg aacggaatgc ctttcttcca ggtcttcccg gaatcggtca
      421 gggagatccc ctgcgagctc gaaccgttgg ccaacaaccc ggaccttgat tacaacctgg
      481 tgatcctggc ctaccaggtc tggtatcttt caccatctat accgatgacc tcctttctga
      541 aaagtgaagc cgcaggtaag atactgaagg gcaaaccggt tatcaccgtc ctgggggtga
      601 ggaacatgtg gatcatggcg caggagaggg tgaaggcgat gattgccggg gccggaggaa
      661 aatactgcgg aaacatagtg atggctgatc cggccggcaa cctgaccagc gtgatcacca
      721 tcgtcaggtg gatgatgaaa ggggaaaaaa cgccgttccg ctggatggga gtgaactttc
      781 cggctgccgg tgtaccgtat gaggagatag ggagagggag agaatatgga agattgatta
      841 tgcactattt aataagtagc ttcacaggag aattgcagga ggatcttgtc cggcaaggtg
      901 cggtaaaagt taatccggtg ttgtacaaga ttgaaaagac cggcaaacgg atcttcggga
      961 tctggtcgaa gctggttctg gccaaaggcg gctacaacga tccggcccgc gaagggaggc
     1021 tgaaagcatt ccaatactac ctttttgcgg ttatttacct ggtctcgccc atcgttgccg
     1081 ccgtaaaccg cttcctgttt tttttgaatc ccggcgctgc cagaaagatt gttgaccgat
     1141 tctccaggtg agatggcatc tcgaattcca tgagactcca tctcaattgg ccagatggcg
     1201 tctcgtttca ttggagactc catctcaaaa atcgagatgg agtctcacta tttaaaatct
     1261 cccggaaaat tttaactttg cccactcaaa atactttgat gcggcttgta tatgatcaat
     1321 gaggtttaca tcacccgact ctccaaattc ctgcccaata aacctgtgga taacgaccgg
     1381 atggaagatt tcctgggaat gatcgacggg aaaccgtcnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnct gtggataacg accggatgga agatttcctg ggaatgatcg
     1561 acgggaaacc gtctaaagca aaacggatca tcctgagaaa caaccagatc acccaacgtt
     1621 attatgccct cgatgaaaag ggcaaactta cccataataa cgcacaactc accgcagcag
     1681 ccatccgcct gctgtttgat aaagatttta ctgctgaaga tatagagctt ctggcctgcg
     1741 gcaccacatc ccccgatcag acgctccctt cgcacgcctc gatggtgcac ggagagctgg
     1801 gcggcaaacc gctggagatc atctccccct ccggctcctg cgccaccggc atgcacgcca
     1861 tgaagtatgc ctacctggcc gttaaagcag gcgaaaagaa caatgctgtt tgttccggct
     1921 ccgaactttt ctcgatcatg atgcaggcag acaaatttga gaaagagacc gaaaatctcc
     1981 acctgcttga tcagaatcct atcctggcct tcgaaaaaga gttcctccgc tggatgctga
     2041 gcgacggcgc cggcgccgcg ttgttacaga acagtcccaa tccggatggc ctgtcgctca
     2101 ggatcgactg gattgagtcg gcatcctttg ccggccggct ggaaacctgc atgtacagcg
     2161 gcggcgaaaa aatgcctgac ggaaacgtaa aaggctggaa agagttcgat tcacaggaca
     2221 tcctgaacaa atccctcttt tccctccacc aggatgtgaa attgcttggc gaaaacattt
     2281 accagacctg caatatgttc ctggcagata tcgtgaaaag aagaaacctc gacctcaacc
     2341 aaatcgatta ttttctgccg catatctcat ccgagtattt tcgttatcgt atcgatgccg
     2401 acagtataga aaaaggagtt catatcccgc aggaaaaatg gttcacaaac ctggtcaggc
     2461 tgggcaatgt cggcgccgct tccatctact tcatgctcga agagatcttc cattccggaa
     2521 ctttgaaaaa agggcagaaa ctgcttctcg gcgttcccga aagcgcccgg ttctcttatg
     2581 tttacagcct ggtaactgtc gtttgacttc ttttattatc tttgccccca ctttgaaaat
     2641 gagccaccct gatgctggtt ccgaaagaaa agattatgga attgctgccc cagcgccccc
     2701 caatggttat ggtggacgaa ctggtaatgt gcaatgaaat ggaagcggtc tccaggcttt
     2761 ttatccgtga tgacaacatt tttttaggag aaaccggttt ttcaccttcc gggatgctcg
     2821 aagccatggc tcaaacagcc gccgccagaa cgggctacct gttgaaaaaa cagccggaga
     2881 gcgcacacaa aagtatacct gtcggtgtta tcgggagtat aaagaatttt aagcttcatt
     2941 ttcaacctgg atcaggtgat actattttta ctacggtttt cgtggaacat gaagttttac
     3001 aggcaactgt gatcagggca aaagtggagg cagagggcaa actcgctgcc gaaagcaacc
     3061 tgcagatttt tttatcggaa gaacaaactt aaccatgaag aagaaagagg ttccccagga
     3121 tgaaggtctg tcggacggtt tgttcgaaga catctgctat gcgctggacg acgatggaca
     3181 ttatgtcgtt gtcccgacaa caggctttca gcccaagacc gatgctttgc tgcaggcatg
     3241 ggatgttatc catgaaaaag ttgaagcggt caggcaacag gtattggcag gcaatcttag
     3301 cccaatcgct tatttcatgg aaaaaaacat tatggatgcc cggttgcttg ccgattatac
     3361 ggagcttccg aaatggaaag tcaggcggca tcttaaaccc aaatatttta acaggctgga
     3421 taattcgatc cttgaccgtt acgcggatac attcgggatc agcagggaac aactgatgga
     3481 catccggact atacaggagg ataaacatga gccaggataa attactcatc gactttcagc
     3541 ataaaccggc tgcacattgc gaaaacggag ccacttccaa tctgctgaac cattacaaca
     3601 tccgtctcag cgaaccgatg gtgttcggca tcggttcagg cctgtttttc tcctatatgc
     3661 cgtttttaaa actcaatggc atccccgtag tttctttcag accgtggccg ggaaccatct
     3721 ttaatcgcaa tacccgcagg ctcggtattg aaattcagc
//
LOCUS       DAOQXS010000307         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25290_length_3754_cov_4.487159, whole genome shotgun sequence.
ACCESSION   DAOQXS010000307 DAOQXS010000000
VERSION     DAOQXS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25290_length_3754_cov_4.487159"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2998)
                     /locus_tag="PKM34_08790"
     CDS             complement(<1..2998)
                     /locus_tag="PKM34_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNQ83726.1"
                     /translation="MYKKFIITLSCLSLIFPLTAAGQTPALHFDRITTENTLTVKGLS
                     QNSIYCLIQDSRGFIWIGTWDGLNRYDGYDYIVYNTANGLSHPTINSVIEDDEQNIWI
                     GTDDGLNRLNRSTGKITVFHHNPENPNTLSNDYVNHILQDKQGYLWISTADGLNRYDK
                     QQGTFTVYNFYSKNTDSTISNFISCVEEYDAGTIWIGTHSGIHRFDIKNQEFKKYKLP
                     GVPGSADFLRSNYIRDLVADDYGQVYAGTLNGVFLLNPIYGVILHLEATDHRNLQLSS
                     DQVNSLLLDRKGVLWIGTNKGLNMFNPITDKVDRIVSGINSYNLSNEDIRSIYQDNSG
                     TIWVGTYKGLNKVDLHPGRFLHLRNDPEDPNSLSDNIVYSILEDEKGLLWIGTYDGVN
                     LFDREKGQFTFLKNRPGDYRSLSSNKIRTLALDSAGFLWIGTESDGINRLDRQSGDIK
                     RFYNRENDNSTIAENNILSTMVDSKGRIWIGTVSKGLTIINSSGQVIRHISARPGSDP
                     RLSDDKIWVVYEDKQGNFWIGSNIGVHKLSPDLNLTGVYRHDSADPNSISSNMIFSIL
                     EDREGIFWMGTMGGGLNRYDPVTQTFKTYNENSGLPNNVVYTALEDEDGNLWITTNRG
                     VSKFDKRTQSFVNYDTQDGVQGNEFNTGAYFKNEKGEIFFGGMSGINIFHPNSIVLNK
                     IPPRMAFTGLKVLNDLVSTDLENGEVIRLNYNENFFAISFSGLDYTNPSKNIYRYKLE
                     NYDEEWIVAGASRRTAEYRKVEPGTYRFIVTGANNDGVWNEKGIGLTIVVRNPWWRTW
                     VFRLLSALFLITVAWSIVLVRIRSVRKKHDVEKKMLAIEKQVFELEQKALRLQMNPHF
                     IFNSLNAIQNFVLANDTDRAVNYLAKFSHLMRMILANSTASLITLKDELRALTHYIDL
                     EKLRFDDKFDYVITHDPDLDEEFVEIPPMLFQPYVENAIIHGLVNSPRKGLLEVSIKK
                     ISQGTLLCSIRDNGVGRDKAIELRNMS"
     gene            3153..3623
                     /locus_tag="PKM34_08795"
     CDS             3153..3623
                     /locus_tag="PKM34_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83727.1"
                     /translation="MKIKTLFISILFITLIGFGAGCGKDDGDRLPLPPTDITIDPNST
                     IYQELNIVGGWTYLGIQDGVEPPSRGIIVYRLSMDQFMAFERTPPFKPDSCCSNSGTV
                     CTSLVVDNFYPFVMDTCTNSKYLILDGSPVSGPSTMTLGIYITEYYANQLYIHD"
     gene            complement(3628..>3754)
                     /locus_tag="PKM34_08800"
     CDS             complement(3628..>3754)
                     /locus_tag="PKM34_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNQ83728.1"
                     /translation="EEVARLHLEHIGVQLTELSQEQADYIGVPKNGPFKPEHYRY"
BASE COUNT          948 a          906 c          835 g         1065 t
ORIGIN      
        1 ccgacatatt cctcagttcg atagccttat cccggccaac accgttgtca cggatactgc
       61 aaagcaacgt gccctggctg atctttttga tggagacttc tagtagtcct ttcctggggc
      121 tgttgaccaa tccgtgaatg atggcattct ccacataggg ctggaagagc attgggggta
      181 tttcgacaaa ttcctcatcc agatcgggat catgcgtgat cacgtaatcg aatttatcat
      241 caaaacggag tttctccaga tcgatataat gagtcagggc ccgtaattca tctttaagtg
      301 taatcagcga ggcggtcgaa ttggccagga tcatccgcat caggtgtgaa aatttggcca
      361 ggtagttcac tgcccggtct gtatcattgg ccagcacaaa gttctgaatg gcattcagtg
      421 aattaaagat gaaatgcgga ttcatctgca atctcagggc tttttgttcc agttcaaaga
      481 cctgtttttc gatggccagc atttttttct ctacatcatg tttctttctg acggatctga
      541 tacgtaccag gacaatactc caggccaccg tgatcaggaa gagcgcagaa agcagccgga
      601 agacccaggt cctccaccag ggattccgga caacaatggt caggccgatg cctttctcat
      661 tccagacccc gtcgttattg gcccctgtca caatgaaccg gtaagtgccc ggttccactt
      721 tacggtattc ggccgttcgc cggcttgcgc cggccacgat ccattcctcg tcataatttt
      781 caagcttata acggtatata ttcttcgacg gatttgtata atccaggccc gagaacgaaa
      841 tagcgaaaaa gttttcattg tagttcagcc ggatgacctc accgttttcc aggtcagtac
      901 tgaccaggtc attgagaacc ttcaggcctg taaatgccat ccggggaggg atcttgttca
      961 ggacgatgct gttaggatga aaaatattaa tgccgctcat accaccgaaa aatatttccc
     1021 ccttttcgtt tttaaaataa gccccggtgt tgaattcatt tccctgcacc ccgtcctgcg
     1081 tatcataatt gacaaatgac tgggtccgct tatcaaattt cgaaacaccc cggttggtcg
     1141 taatccacag gttcccatct tcatcttcca aagccgtata aaccacatta ttgggaaggc
     1201 cgctattttc attatacgtc ttaaacgtct gtgtaaccgg atcataccgg ttcagaccgc
     1261 ctcccattgt tcccatccag aaaattcctt ccctgtcttc cagaatcgaa aagatcatat
     1321 tgctgctgat cgaattcgga tcggccgaat catgcctgta aacgccggtt aaattcagat
     1381 cgggcgacaa tttatgcacg ccaatattcg acccgatcca gaaattgcct tgcttgtctt
     1441 cgtaaacaac ccagatttta tcatcagaca gcctgggatc ggagccgggc ctggcagaaa
     1501 tgtgcctgat cacctgcccg gaagaattaa taatggtcaa tcctttgctg accgtaccta
     1561 tccatatccg tccttttgaa tcgaccatcg tggatagaat attgttctcg gcaatggtgg
     1621 aattgtcgtt ttcacggtta tagaaccgtt taatatcacc tgattgccgg tctaaccggt
     1681 taattccatc actttccgtc ccaatccaga ggaaaccggc actatccagc gccagtgtcc
     1741 tgatcttatt gcttgacaag cttctgtaat cgccaggcct gttttttaaa aaggtgaact
     1801 gacctttttc cctgtcaaac aggtttacac cgtcgtaggt accgatccac aagagccctt
     1861 tctcatcttc caatattgag taaacaatat tatcggacag gctgttggga tcttccggat
     1921 cattcctgag atgcagaaac cggccgggat gcaaatcaac tttattcagg cctttgtaag
     1981 tgccgaccca gatggtcccc gaattgtcct ggtaaatact tctgatatct tcgttgctca
     2041 gattataact attaatgccg gaaacgatcc ggtccacctt atccgttatc gggttgaaca
     2101 tgtttaagcc tttattggtc ccgatccata ataccccctt ccggtctaaa agaagtgaat
     2161 tgacctggtc ggacgacagt tgcaggttgc ggtgatcggt tgcctccagg tgaaggataa
     2221 cgccgtagat cgggttgagc aggaagaccc cgttcagcgt tccggcgtat acctgtccgt
     2281 aatcatcggc aaccaggtcg cggatatagt tgctccgaag aaagtcagca gaaccgggca
     2341 caccgggtag tttatatttt ttaaattcct ggtttttaat atcaaaacga tggatgccac
     2401 tgtgtgttcc gatccagatg gttccggcat cgtactcctc cacacacgaa atgaagttgg
     2461 aaatggtact gtcggtattt ttggaataaa aattataaac agtaaatgtt ccctgttgct
     2521 tatcatacct gttgaggcca tcggcagtcg atatccagag gtatccctgc ttatcctgca
     2581 gaatatgatt aacataatcg ttactcaggg tattcgggtt ctccgggtta tgatggaaaa
     2641 ctgtgatctt tcccgtcgac cggttcagcc ggttcaggcc gtcatcggtt cctatccaga
     2701 tattttgctc atcgtcttca ataaccgaat ttattgtcgg atggctcagt ccgttggccg
     2761 tattgtaaac gatataatca tatccgtcat agcggtttaa tccatcccag gtgccaatcc
     2821 agataaaacc ccgtgagtcc tggatcaggc agtaaatgga gttttgagac agccccttga
     2881 ccgtaagcgt attttcagtg gtgatccggt caaaatgcaa tgcaggcgtc tgtcctgccg
     2941 cagtcaaagg aaagataagg ctcaggcagg aaagggttat gatgaacttt ttatacacgc
     3001 tcttccggag taaatataat gctaaaataa ataatttaac agtccggtta acggattggc
     3061 ctttccggcc gacaatatgc agaattttca gtagtctatg ccaaaggtat gatctaattt
     3121 actgtatttt tgaattttta accgggattc aaatgaagat caagactctg tttataagca
     3181 tactttttat cactttaatc ggtttcggtg cgggatgtgg taaagatgac ggtgaccggc
     3241 tgcctttgcc tccgaccgat attaccattg atccgaattc gacgatttac caggaactga
     3301 atattgtcgg gggatggaca tacctgggaa tccaggacgg ggtggaaccg cccagccgcg
     3361 gcattatagt atacaggctc agcatggatc agttcatggc ttttgaacgg acccctcctt
     3421 tcaaacccga cagttgctgc agcaattccg gcaccgtttg tacaagcctg gtagtcgata
     3481 atttttaccc gtttgtgatg gatacctgta ccaattccaa gtaccttatt cttgacggtt
     3541 cgccggtatc aggtccgtcg accatgaccc tgggcattta tatcacggaa tattacgcaa
     3601 accagcttta tattcatgac taagtaatta gtatcggtag tgttccggtt taaaggggcc
     3661 atttttgggt acaccgatat aatcagcctg ttcctgcgat aattcggtaa gttgaacccc
     3721 aatatgttcc aggtgcaaac gggcgacttc ttca
//
LOCUS       DAOQXS010000308         3752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25307_length_3752_cov_3.424398, whole genome shotgun sequence.
ACCESSION   DAOQXS010000308 DAOQXS010000000
VERSION     DAOQXS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25307_length_3752_cov_3.424398"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1066)
                     /locus_tag="PKM34_08805"
     CDS             complement(140..1066)
                     /locus_tag="PKM34_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83729.1"
                     /translation="MKTKSTLTLITAIWITILMIAGTRAQSQENIIVQIGESSMSKGL
                     QTCYSVRIPQAEIKAVQQGWIKHLQEDNKAKVKEIGHELHFSGGLKTEIFPDTISLYS
                     LLIATDSAINLNVFLEIDSVFFTPNSDKTDLASDKIDNSLRNYIRNFAVDQYRIAVED
                     ELKAAEKVLKDLQNDLEKLQKDEKNLLNDNVKMENEIESTELELTQFDNSLAIKNQEI
                     IDHNARMQNIALETEKEAAKAKQKELEKEKNQLEKSRGKAKELISDNKSQIEKNKKAI
                     KQSQEDQDLKQQDITKQNNLIMVVQNKLKGIK"
     gene            1213..2100
                     /locus_tag="PKM34_08810"
     CDS             1213..2100
                     /locus_tag="PKM34_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83730.1"
                     /translation="MKALFHKKFLEHNIDSDSEGAYRLLSFMDTYEAPEVLGEPYINL
                     VHSERYIEYIREACENGDFVAEVALTPASYEAACLAVGMTIRASEQGDFAVVRPPGHH
                     AGREKAAGFCFFNNIAIAVQKLVNEGKKVFIFDIDGHHGDGTQSIFYDSDRVLYCSIH
                     QHYAYPYTGFPDETGDGSGKGFNFNFPLISGSGDKDFLIAVDKAIDVARKFNPDVVAV
                     SAGFDAYYKDKLLGLQVTQRAFYECAFRTRRAFSNIFAVLEGGYHNDVRECIDNFIEG
                     IHNGAKPPVLKWDTDMSIG"
     gene            2103..3002
                     /locus_tag="PKM34_08815"
     CDS             2103..3002
                     /locus_tag="PKM34_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ83731.1"
                     /translation="MNLSEHYGELAALATAMFWTITALSFEFASKRVGSLAVNMIRLT
                     FAFILLGIFTLITRGMFLPLDATSFNWMWLALSGLIGLVLGDYFLFKSYTVISSRVSM
                     LIMTTVPPMTALIGWLLLGEKMIPMHILGMFLTVGGIFLAIFSRPVDGGRIRLSYPAK
                     GILFALVGAAGQAGGLVLSKYGMAGYNSFAASHIRLIAGMAGYAMLILFLGKGGLIRK
                     AIKDGKGMRAIGLGSFFGPFLGISFSLLAVKYTETGIAATIMAIVPILIIPPSVWLFK
                     EKVTWMEIAGAVISVGGVVLMFL"
     gene            3340..>3752
                     /locus_tag="PKM34_08820"
     CDS             3340..>3752
                     /locus_tag="PKM34_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ83732.1"
                     /translation="MKINIKYLRISPLIFIFIILTSIPVITNAQDGLKTKTKVRPTVG
                     LVLSGGGAKGFAYIGLLKVIHEAGLPLDYIGGTSIGSIMGGLYALGYHPDTIDTMIRS
                     IDWDALLADKIERKYISFEEKEFGEKFIITLPVKS"
BASE COUNT          874 a          872 c          889 g         1117 t
ORIGIN      
        1 aggtcattcc aggagatgat aacgtaggaa tcatcaaccg gtggtttatt tgcaaccaga
       61 acagcaatag ctcctgtaac aagcaggact gcaaaaactc tgaaagaagc ctttttcata
      121 ggtagttttt ttaattaatc tatttaattc ctttaagttt attttgaaca accatgatca
      181 aattattttg cttggtgatg tcttgctgct ttaagtcctg gtcttcctga ctttgcttta
      241 tcgctttttt atttttctct atctgtgatt tgttatcgga gatcagttct ttcgctttgc
      301 ccctgctttt ttccagctgg ttcttctctt tctccagttc tttttgtttg gctttggccg
      361 cctctttttc ggtttcaaga gcgatattct gcatccgggc attatggtcg ataatttcct
      421 ggttcttgat ggccaggctg ttatcgaatt gcgttaactc gagttctgtc gattcaatct
      481 cattttccat tttgacatta tcattcaaaa ggttcttttc atccttttgt aatttttcca
      541 gatcattctg caaatctttc aatactttct ccgccgcttt gagctcatcc tccaccgcaa
      601 tcctgtattg atcgacggca aagttccgga tataatttct aaggctgttg tcgatcttgt
      661 cgctggccag gtctgtctta tcggaatttg gtgtgaaaaa aacgctgtcg atttccagga
      721 acacattcag gtttatagca ctgtcggtag cgatcaacag gctgtaaagg cttatcgtat
      781 ccggaaaaat ttctgttttg agcccgcctg agaaatggag ttcgtggccg atctctttta
      841 ctttggcttt gttgtcttcc tgtaaatgtt tgatccagcc ttgctggact gcttttattt
      901 ccgcctgggg aattctgacc gaataacagg tttgtaaccc ttttgacatg gaggactctc
      961 caatctgtac aatgatgttt tcctgggact gtgcccgggt gccagcaatc atgagtattg
     1021 tgatccaaat ggcagtaatg agggtaaggg ttgatttcgt tttcataatc agtttattta
     1081 aaaggataaa tgtactgcat ttggatcggt tttccaagaa ttgagcccga taaatgccca
     1141 tactgtttaa aaacaattga attattgtaa tgaattagta aatttgttcg atctagatct
     1201 ggttaaagga gcatgaaagc attatttcac aagaaattcc tcgaacataa catcgacagc
     1261 gacagtgaag gagcctaccg tttgctaagc tttatggata cctatgaggc gccggaggtg
     1321 ctgggggagc cgtatatcaa cctggtccat tccgaacgat atattgaata catcagggaa
     1381 gcctgtgaaa atggggattt tgtggctgaa gtcgcactta ctcctgccag ttatgaggcg
     1441 gcttgcctgg ctgttgggat gacgatcagg gcttcggagc agggtgattt tgccgttgtg
     1501 cgccctccgg gacatcatgc cggacgtgaa aaagcggccg gtttctgctt tttcaacaat
     1561 atcgcgattg cggtccagaa actggtcaat gagggtaaga aggttttcat attcgatatc
     1621 gacgggcatc atggcgacgg aacacagtct attttctatg attccgaccg cgttctttat
     1681 tgttctattc accagcatta tgcatatcca tatactggct ttcccgatga aacgggagat
     1741 gggtccggta agggatttaa tttcaatttt cccctgattt cgggaagcgg agataaagat
     1801 tttctgattg ccgtcgataa agccatcgat gtggcgcgga agttcaaccc tgatgtggtc
     1861 gcagtttccg ccggattcga tgcttattac aaggataaac tcctgggttt gcaggtaaca
     1921 caaagggcct tttacgaatg cgctttccgc acccgccggg ccttttcgaa tatcttcgcc
     1981 gtactcgaag gcggttatca taacgatgta agggaatgta tcgataattt catcgagggt
     2041 atccacaatg gcgccaaacc gcctgtactg aaatgggata ccgatatgtc gatcgggtag
     2101 gaatgaattt gtctgagcat tacggtgaac tggccgcact ggcgacggca atgttctgga
     2161 ccataaccgc cctttctttc gaatttgcca gcaaacgcgt cggatcgctc gccgtgaaca
     2221 tgatccggtt gacatttgcg tttatcctgc tcggcatctt taccctgatc acccgcggca
     2281 tgttcctccc gctcgacgca acatcgttca actggatgtg gctggcgctg tccggtctca
     2341 tcgggcttgt cctgggcgac tacttcctgt ttaaatctta tacggtcatt tcctcccgcg
     2401 tctccatgct gatcatgacc accgttccgc cgatgacggc cctgatcgga tggctgctat
     2461 taggggaaaa gatgatcccg atgcacatcc tgggcatgtt ccttacggtc ggtggcattt
     2521 tcctggccat attttcccgt ccagtcgatg gcggccgcat ccgcctttcc tacccggcca
     2581 aagggatttt gttcgccctg gtcggagctg ccgggcaagc cggaggactt gtgctgagca
     2641 aatacggtat ggccggctat aactcatttg ctgcctccca catccggctg atcgccggca
     2701 tggcagggta tgccatgctc atcctgttcc ttggaaaggg aggactgatc agaaaggcaa
     2761 ttaaagacgg gaaagggatg cgagccatcg gacttggctc gttcttcggc ccgttcctgg
     2821 gaatctcctt ctccctattg gctgtcaaat acaccgaaac ggggattgcc gcgacgatca
     2881 tggctatcgt ccccatcctg atcattcccc cgtctgtctg gcttttcaag gaaaaggtga
     2941 cctggatgga gatcgccggt gcggtgatca gcgtcggtgg cgtggtgctg atgtttttgt
     3001 aaaaaggcgg atttcgaatt tcagatgcga aatgctttca gatccttcgc tgcgctacca
     3061 atgacaagtt agtaaatttc tactgaactg ctctcctccg gagtaatgaa aagctaatgt
     3121 catccaatat tgtggagttt gtaaaagtct gaaaactcag gatgacggtt gccgcgccga
     3181 ccattcgttt gcgcggttct gccgtcatcc tgagcgcagc gaaggaactg ccaatttgtg
     3241 gaaattcctt ccccaatcca gtatcgagtt tcgattatct taaaaactgt ccggcaaatt
     3301 tcaatatctt tacctgccga tcattcttgg ccaaacatca tgaaaatcaa tataaaatat
     3361 cttagaattt ctcctctcat tttcatattt attatcttaa catctattcc ggttatcact
     3421 aatgcccagg acgggctgaa aacaaaaacc aaagttcgcc ccaccgtcgg tctggttctc
     3481 agtggaggag gagcaaaggg ctttgcttac atcgggctgt taaaggtcat ccacgaagcg
     3541 ggcctgccac tggattacat cggggggacc agcatcggaa gcatcatggg cgggctctac
     3601 gccctgggat accacccgga tacgatcgat accatgatac gctccatcga ctgggacgcg
     3661 ctgctggccg ataagatcga acgaaaatat atctcttttg aagaaaaaga gttcggggaa
     3721 aaattcatta tcacccttcc ggtaaaaagt aa
//
LOCUS       DAOQXS010000309         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25474_length_3737_cov_4.016295, whole genome shotgun sequence.
ACCESSION   DAOQXS010000309 DAOQXS010000000
VERSION     DAOQXS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25474_length_3737_cov_4.016295"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..254)
                     /locus_tag="PKM34_08825"
     CDS             complement(18..254)
                     /locus_tag="PKM34_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNQ83733.1"
                     /translation="MKKGVSNFLLGFLAGAAAGALAGILLAPDKGSETRKNLKKKVKD
                     LSDEYNLGLSELMDDMEPEKAKAGKREKGKRNSE"
     gene            complement(258..1352)
                     /locus_tag="PKM34_08830"
     CDS             complement(258..1352)
                     /locus_tag="PKM34_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ83734.1"
                     /translation="MSQKYRVIVAATGVLLGLFMAWYFFNILVYIITAGVISLTGQPL
                     VEALSRIRIGRYSMPKSIAALITIVVLIGFLTGLVFLFVPAISRQAHIISMINVDEVV
                     KYFADVIDKMQRLLLSYDLIGTDQTLQSLLDQQIRSVIKVTDISNIIEKLVGATTQVF
                     IGIFSVLFLAFFFLRDRQLLRNGILLFTPDKFQEEVKNILHKTKTMLSRYFIGLVSEL
                     LIMMILISIGLSLFGIKNAVFIGFIGGLMNVIPYLGPIIGATIGAILGISADLSLGMY
                     DNALNTALIVVAVFSVANLIDNWVLQPVIYSRSVNAHPIEIFIVILMAGSLAGIPGMI
                     LAVPGYTVLRIVAKEFLSGFKLIDKLTENI"
     gene            complement(1395..1760)
                     /locus_tag="PKM34_08835"
     CDS             complement(1395..1760)
                     /locus_tag="PKM34_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNQ83735.1"
                     /translation="MKGLTTIGMLILSNAFMTMAWYGHLKFNGAKAGGTGLFIIILTS
                     WGIAFFEYCFQVPANRIGYQGFGGPFTLVQLKVIQEVITLVVFMVFTLLIFKNETIKM
                     NHLIGFVFLVLAVYFIFKK"
     gene            complement(1788..>3737)
                     /locus_tag="PKM34_08840"
     CDS             complement(1788..>3737)
                     /locus_tag="PKM34_08840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNQ83736.1"
                     /translation="PESGYRSAYAHANEMAQPAYYKVRLDDDGILAELTATERVGFHR
                     YTFSKNDTANIILDLIHGIYNYEDKNVWTFIRVENDTLVTGYRQTSGWARTRTVYFAM
                     AFDNPVVEYGHRKYDNAVYRGFYRKFDETKNFPEMAGEKIRAYFRFLLHEGESLRVKF
                     ALSSTSTEGALKNLQTEIPHWDFDRIVNESQAKWNKELGKVAVEMMTPADMTTFYTAL
                     YHTFLSPTIYMDVDGAYRGLDQNNHVADGFTNYTTFSLWDTYRALHPLFNLVQPSRNS
                     DMIRSMLAHYDQSVHPMLPVWSHHANENWCMIGYHAVSVIADAAVKNVGNFDRLHALE
                     ACVKTAGYDLFDGIKYYETLGYVPEDKNSSSVSKTLEYAYDDWTIAQLARITGNAEVE
                     KQFAERSLNFRKVYDKEIGFMRPRLASGEWKTPFDPLNTHGQGFIEGNAWNYSLYVPH
                     RIPDMITMMGGPERFTARLDSLFIMHLPDENFKDTEDITRDGIIGCYVHGNEPGHHIP
                     YLYNRAGKPWKTQEKVRMIMREMYGDTPDGLCGNDDCGQMSAWYVFSALGFYPVCPGS
                     DMYAIGSPLVVSAKITLENGRKVGISVENQSEENIFIRKIILNGDEYDKNYFSHSNLV
                     NGAEITFVMGARPEKSWGKISPEDE"
BASE COUNT          865 a          951 c          983 g          938 t
ORIGIN      
        1 tccttattcg ttattcacta ttcactattc ctttttccct tttccctctt ccctgctttc
       61 gccttctccg gctccatgtc gtccatcagt tcgctcaggc cgaggttgta ttcgtcggaa
      121 aggtctttga ccttcttctt caggttctta cgggtttcgc tgcctttgtc gggcgccagg
      181 aggatgccgg caagagcgcc ggcagcggcg ccggccagga aaccgagcaa aaagttagat
      241 accccttttt tcatcgctca aatattttcg gttaatttat caatcagttt aaatccgctc
      301 aggaactcct ttgccacgat cctcagaacg gtatagcccg gcacggcgag gatcatcccg
      361 ggaatgccgg cgaggcttcc cgccatcagg atcacaatga atatttcgat cggatgggcg
      421 ttgacgctcc gcgagtagat caccggttgc aatacccagt tatcgatcag gttggcgacg
      481 gaaaagacgg ccaccacgat cagggcggta ttcagggcat tatcatacat tcccaggctc
      541 aaatctgccg aaatgccaag gatggccccg atagtggccc cgatgatcgg ccccaggtaa
      601 gggatcacgt tcatcaaccc tccgataaaa ccgatgaata cggcattttt tatcccgaat
      661 aaagataatc ctatggagat caggatcatc atgatcagca actccgatac cagcccgata
      721 aaataccgcg acagcatggt cttggtcttg tgtaaaatat tcttcacctc ctcctggaat
      781 ttatccggtg tgaagagcag tatcccgttg cggagaagct gccggtcgcg caggaaaaag
      841 aatgcaagga agagtacgga aaagatcccg atgaacacct gggttgtagc gccgacgagc
      901 ttttcgatga tgttgctgat gtcggtcact ttgattaccg accggatctg ctggtcgagc
      961 aggctctgca gggtctggtc ggtgccgatg aggtcgtagg agagcaacag ccgctgcatc
     1021 ttgtctatca catcggcaaa gtatttcact acttcgtcca cgttgatcat ggagatgatg
     1081 tgggcctggc gggagatcgc ggggacgaag aggaagacca gcccggtcag gaaaccgatg
     1141 aggacgacga tcgtgatgag ggcggcgatg gatttgggca tggaatagcg cccgatcctg
     1201 atcctcgaca aggcttcgac cagcggctgt ccggtcagtg agatcacccc ggcggtgatg
     1261 atgtacacga ggatattaaa gaaataccag gccatgaaaa ggccaaggag aacgccggtt
     1321 gcggcaacga tcacacggta tttctggctc atcggatgaa ttcctcggtt aacaaaaata
     1381 agaatataat gacgttattt tttaaaaata aaatagacgg cgagcacgag aaacacgaat
     1441 ccgatcaggt ggttcatttt gatcgtttca tttttaaaga tcagcagggt aaaaaccata
     1501 aaaacgacaa gcgtgatcac ctcctggatg acttttagct gtaccagcgt gaacgggccg
     1561 ccgaatccct ggtagccgat ccggttggcc ggcacctgga aacagtattc aaagaaggca
     1621 atgccccagc tggtcaggat gatgatgaac aggcccgtgc cgcccgcttt cgccccgttg
     1681 aacttcaggt ggccgtacca ggccatggtc ataaatgcat tcgacaggat aagcatcccg
     1741 atggttgtca gacctttcat cgtaaaaata atttgattcc gaattattta ttcatcttcc
     1801 ggtgagatct tcccccatga cttttcagga cgagctccca tcacaaaagt aatttcagcc
     1861 ccgtttacaa ggtttgaatg cgaaaaataa tttttatcat attcatctcc gttaagaatt
     1921 atttttcgaa tgaatatgtt ttcttcggat tgattctcaa ctgaaattcc gaccttccga
     1981 ccattttcga gggtaatttt ggcggatact accagcgggc tgccgatggc atacatatcg
     2041 ctgccggggc atacggggta aaagccgagg gcgctgaaga cataccaggc cgacatctgg
     2101 ccgcagtcgt cgttgccgca aagcccgtca ggcgtatcgc catacatctc ccgcatgatc
     2161 atcctgactt tttcctgcgt cttccagggt ttcccggccc ggttgtagag atacgggatg
     2221 tgatggccgg gctcgttgcc atggacatag cagccgatga tcccgtcgcg cgtgatgtct
     2281 tcggtatcct tgaagttctc atccggcaag tgcatgatga acagcgaatc gaggcgggcg
     2341 gtgaaacgtt ccgggccgcc catcatggtt atcatatcgg gtatccggtg gggcacgtaa
     2401 aggctgtaat tccaggcatt tccctcgatg aacccctggc cgtgggtgtt cagcgggtcg
     2461 aacggcgttt tccattcgcc tgatgccagc ctggggcgca taaaacctat ctctttatcg
     2521 tacaccttcc tgaaattcag cgaccgttca gcgaactgct tttccacctc cgcattgcct
     2581 gtaatccttg ccagttgggc tatggtccag tcgtcgtaag cgtattccag tgtttttgat
     2641 acggaggatg agtttttatc ttccggaaca taacccaatg tctcataata ttttataccg
     2701 tcaaagagat catacccggc tgtttttaca caagcttcga gggcgtgcaa acggtcgaaa
     2761 ttccccacat tcttgaccgc ggcatcggcg atgaccgaaa cggcgtgata accgatcatg
     2821 caccagtttt cgttggcgtg gtgcgaccac accgggagca tcgggtggac gctctggtcg
     2881 taatgggcca gcatggagcg gatcatatcg ctgttgcggg aaggctgcac caggttgaac
     2941 agcgggtgca gcgcgcggta ggtgtcccag agcgaaaagg tcgtgtaatt cgtgaacccg
     3001 tcggcgacgt ggttattctg gtcgagaccg cggtaggcac catcgacgtc catgtagatt
     3061 gtcgggctaa ggaaggtatg atagagagcc gtgtagaagg tggtcatgtc ggccggggtc
     3121 atcatttcga cagcgacctt gccgagttct ttattccatt tcgcctggct ttcattgact
     3181 atcctatcaa aatcccagtg aggtatctcc gtctgcaggt tttttaatgc accttcggtg
     3241 gatgtggagg atagggcgaa tttcacccgt aaactttcgc cttcgtgcaa aagaaatctg
     3301 aaatacgccc tgatcttctc cccggccatc tccgggaagt tcttcgtttc gtcgaatttc
     3361 ctgtaaaacc cgcggtaaac ggcattgtcg tattttctgt gcccgtattc gacgaccgga
     3421 ttatcgaagg ccatggcaaa atagactgtc cgggtcctgg cccatccgct ggtttgccgg
     3481 taacccgtga cgagggtgtc gttttccacc cggataaagg tccagacatt tttatcctcg
     3541 taattgtaaa tgccgtggat cagatcgagg ataatgttgg cggtatcgtt ttttgaaaag
     3601 gtatagcgat ggaaacccac ccgttccgta gccgtcagtt ctgcaagaat gccgtcgtcg
     3661 tcgagccgca ccttgtagta agccggctgc gccatttcgt tggcgtgcgc ataggccgac
     3721 cggtagccgc tttcggg
//
LOCUS       DAOQXS010000310         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25684_length_3716_cov_3.517618, whole genome shotgun sequence.
ACCESSION   DAOQXS010000310 DAOQXS010000000
VERSION     DAOQXS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25684_length_3716_cov_3.517618"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PKM34_08845"
     CDS             complement(<1..1065)
                     /locus_tag="PKM34_08845"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNQ83737.1"
                     /translation="MKTIDNFNFNGKKALIRVDFNVPLDENYHITDTTRIDAAIPTLK
                     KILGDGGAVILMSHLGRPKEGPAEKYSLKHLLPYLNKTLNLTVKFAGDCIGEEAKEMA
                     GKLKGGEVLLLENLRFYKNEEKGDEEFAAKLAQLGDVYVNDAFGTAHRAHASTAIIAK
                     FFPENKLFGYIMEEEVKAIDKVLRNAERPFTAILGGAKVSGKIEIINNLIDKVDNLLI
                     GGGMMFTFIKALGGEVGSSLVEEDLLQLARETSDKAEANNVKIYIPQDAVIASAFQND
                     AEKMECGSTAIPEGWMGLDIGPKAIAAYREVILNSKTILWNGPMGVFEMPNFEPGTKA
                     IAEAVAEATQNGAFSLIGGGD"
     gene            1178..2518
                     /gene="trkA"
                     /locus_tag="PKM34_08850"
     CDS             1178..2518
                     /gene="trkA"
                     /locus_tag="PKM34_08850"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNQ83738.1"
                     /translation="MRIIIAGDGEVGLYLAQELSKEDHSITMIVPSEDLVKTIESHSD
                     VMATVGDPTLISVLKRSNVKKADLLISGLHEERINILTAALGKKIGAIKTIARINTME
                     YLSDENRRMFEELGVDHLVCPEDIAANEVVDLLRQTAATETFDFSQGKLSLFLLRLEK
                     NAPVLNKTLDQIAKEFVHLHFRAVAIHRDRKTIIPKGDDKFLENDLAYVITKPQGIND
                     LLKLSGKSKISIHNLMLLGGGRVGRTVARRLEHSMNIKLIEKDKDRAIQLANTLDHCL
                     VIHGDARDIQLLEEEDISGMDAFVALTDNSETNILACLHAKKFGVPRTIALVENIDYI
                     DISQNIGIDTIVCKKLSTASNIIRFTLISEVTSIKCLSGINAQVLEVVTKPHSPVTKA
                     PIRKLKFPPGAIIGGIIRGSDSFIAVGDFQIQEGDKVVVFALPDALHAVDNLFH"
     gene            2562..>3716
                     /locus_tag="PKM34_08855"
     CDS             2562..>3716
                     /locus_tag="PKM34_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNQ83739.1"
                     /translation="MFGKSEINFRQILRLIGVILVIEGVFMLTGLPFSLYYQDDKPFT
                     LLYSFIITSASGGILLFFTRSDKDNTINKREGFLVVSLAWVVMSLFGTVPFLLSGAIP
                     SFTDAFFETMSGFTTTGASILSDVEVLPKGILFWRSMTHWIGGMGIIVLTIIILPFLG
                     IGGMQLYVAEMPGVSKDKLHPKISETAKRLWGIYIVLTAIQTILLVLGKMPLLDSLCH
                     AFGSMATGGFSTRNASIGAYSPYIQYVVIVFMFLAGMNFTLHYFIISGRFKEVWKNEE
                     FRKYLYISLGFSIIIAAFLVIKTDAGYAKAFRDALFQVVSIVTTTGYATTDYLVWPGA
                     LWFFIFLMMFVGGMAGSTGGGVKVVRQLLLFKNAGKELKRAIHPQGVIPVR"
BASE COUNT          879 a          955 c          921 g          961 t
ORIGIN      
        1 gtcaccaccg ccgatcagtg aaaaagcgcc gttctgcgtg gcttcggcaa cagcttcggc
       61 aatggctttg gtccccggtt cgaaattcgg catctcgaaa acacccatcg ggccattcca
      121 caatatcgtt ttagagttca ggatcacttc gcggtaagca gctatggctt tcgggccgat
      181 atcaaggccc atccatcctt ccggtatggc cgtcgatccg cattccatct tttccgcatc
      241 gttctggaag gcgctggcaa tgacagcgtc ctgcgggata tagattttaa cattgttggc
      301 ttcggctttg tcgctggttt cgcgggccag ctgcagcagg tcttcctcta ccagcgagct
      361 gcccacctct ccgccaaggg ctttgatgaa ggtgaacatc atcccgccgc cgatcagcag
      421 gttatcgacc ttatcgatga gattgttgat gatctctatc tttcccgaaa ccttcgcccc
      481 gcccaggatc gcggtgaacg gcctctcggc gttccgcagc accttgtcga tggctttcac
      541 ctcttcttcc attatgtaac cgaacagttt gttttccggg aagaacttag cgatgatcgc
      601 cgtagaggca tgtgcgcggt gcgccgtccc aaaggcgtca ttcacgtaaa catcgcccag
      661 ttgtgccagt ttggcggcaa actcttcatc gcctttttct tcatttttat aaaaacgcag
      721 gttttccagt aacagcactt ccccgccttt cagcttgccg gccatctctt tcgcttcttc
      781 gccgatacaa tcaccggcaa attttactgt caggttaagc gttttgttca gatagggcag
      841 gaggtgtttc agcgaatatt tttcggcagg gccttctttc gggcggccca ggtgcgacat
      901 caggatcacc gcgccgccgt caccgaggat ctttttcagc gtggggatgg ctgcatcgat
      961 gcgggtagtg tcggtaatat gataattttc atccagggga acattaaaat cgacccgtat
     1021 cagggccttc tttccgttaa aattgaaatt gtctattgtt ttcatgtcga attggtgtta
     1081 attcactcga gatggagtct ccatcttcgg cactgcaaaa ataggaatat accgggaaaa
     1141 tactctacct ttgcccctta tttaattcgt cattacgatg agaatcatca ttgccgggga
     1201 tggagaagtc ggactctacc tggcccagga attatccaaa gaagatcata gcatcactat
     1261 gatcgtcccc agcgaggatc tggtcaaaac catcgaatca cacagcgacg ttatggcaac
     1321 ggtcggtgat ccgacgctga tctcggtgct gaaacggtcg aatgtcaaaa aagccgatct
     1381 gctgatctcc gggctgcatg aagagcggat caacatcctg accgccgcac tggggaaaaa
     1441 gatcggcgct attaagacca tcgcccgcat caacacaatg gaataccttt ccgatgagaa
     1501 caggaggatg ttcgaggagc tcggcgtcga tcacctggtc tgtcctgaag atattgccgc
     1561 aaatgaggta gtggacctgc ttcggcagac ggcggccacc gaaacgtttg atttttcaca
     1621 gggaaagctt tcccttttcc tgcttcgcct tgaaaagaac gccccggttc tgaacaagac
     1681 gctcgaccag atcgccaaag aatttgtcca tctccacttc agggccgtag ccatccaccg
     1741 cgaccggaaa acgatcatcc cgaaagggga cgataaattc cttgaaaatg acctggcata
     1801 cgtgatcaca aaaccccagg ggattaacga cctgctgaaa ctgagcggga aatcgaaaat
     1861 ctctatccat aacctgatgc tgctcggcgg aggaagggtc gggcgtaccg tagcccggag
     1921 gctggaacat tcgatgaaca ttaaactcat cgaaaaagat aaagaccgcg ctatccagct
     1981 ggccaataca ctcgaccact gcctggtgat ccacggcgat gcgcgcgaca ttcaactgct
     2041 cgaagaagag gatatttcag ggatggacgc atttgttgcc ctcaccgaca actcggaaac
     2101 caatatcctg gcctgcctcc atgccaaaaa gttcggtgtt cccaggacca tagcgctggt
     2161 cgagaatatc gactatatcg acatctcaca aaatatcggc atcgacacta tcgtgtgtaa
     2221 aaaactcagt acagccagta atatcatccg cttcactttg atttcggagg tgaccagtat
     2281 caaatgcctc agcgggatca atgcccaggt ccttgaggtg gtgacaaaac cccattctcc
     2341 ggtaaccaaa gctccgatcc ggaagctgaa atttcccccg ggggccatca tcggcgggat
     2401 tatccgggga tccgacagct tcattgccgt cggcgatttt cagatccagg aaggcgacaa
     2461 ggtggtggtc tttgccctgc ccgatgccct ccacgctgtc gataaccttt tccattagag
     2521 attcagactt tcggaaaaaa taacttgacc tatcccttgg tatgttcggc aaatcggaaa
     2581 taaatttcag gcagattctt cgccttattg gcgtgatcct cgttatcgaa ggtgttttca
     2641 tgctgaccgg attgcccttt tctctttatt accaggatga taaacctttc accctgttat
     2701 actcgttcat catcaccagc gcatcgggag ggattttatt gtttttcacc cgttctgata
     2761 aagacaacac gataaacaag agagaaggat tcctggtggt cagcctggcc tgggtggtaa
     2821 tgagcctgtt cgggactgtc ccgtttttgc tgagcggcgc tatcccttct ttcaccgatg
     2881 ctttcttcga aaccatgtcg ggatttacga caaccggagc ctccattctg agcgacgttg
     2941 aagtgctgcc aaaagggatc ctcttctggc ggagtatgac ccactggatc ggcggcatgg
     3001 ggatcattgt actgacaatt atcatcctgc cattcctggg aattggcggg atgcagcttt
     3061 atgtagccga aatgccggga gtttccaaag ataagctgca cccgaaaatt tccgaaacgg
     3121 ccaaacggtt gtgggggatc tatatcgttt taaccgctat acagacgatt ttgctggttt
     3181 tgggaaagat gcccctgctc gacagccttt gccatgcttt cgggtcgatg gctaccggtg
     3241 gattttcaac caggaacgcc agcatcggag cctattcgcc ttacatacaa tatgttgtga
     3301 ttgtttttat gttccttgcc gggatgaatt ttacccttca ttacttcatt atctccggaa
     3361 ggtttaaaga ggtatggaag aacgaggaat tccggaagta tctttatatc tcactggggt
     3421 tcagtattat aattgccgct ttcctggtaa taaagaccga tgcagggtat gcgaaagcat
     3481 tccgggatgc cctgttccag gtggtttcca ttgtcacgac tacaggatat gccacgaccg
     3541 attacctggt atggcccggc gcgctctggt tttttatatt tctgatgatg tttgtaggcg
     3601 gaatggccgg ctccacaggc ggtggcgtga aagtggtaag gcagttgctt ttatttaaga
     3661 atgcgggaaa ggaattgaaa agagcgatcc atccccaggg ggttattccg gtcagg
//
LOCUS       DAOQXS010000311         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25693_length_3715_cov_3.256557, whole genome shotgun sequence.
ACCESSION   DAOQXS010000311 DAOQXS010000000
VERSION     DAOQXS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25693_length_3715_cov_3.256557"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PKM34_08860"
     CDS             complement(<1..1509)
                     /locus_tag="PKM34_08860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNQ83740.1"
                     /translation="MSQPEYIKRANTDVPCRLEDISKSDYEVFYNLVAELLKKESNHC
                     LAYYPIENEGKLVFYCFIGDDSDQSVTILSHRHNKYGLILKSLTPLCNAMHIFEREIH
                     ETFGVAFEGHPWLKPVRYPFDRYDKAKVMDNYPFFEIESEELHEVGVGPIHAGVIEPG
                     HFRFICNGEKVLHLEIHLGYQHRGIEWLYLDKDSPLQRCILSECIAGDTAVGHALAHA
                     QLIESLAGIQIEEDLQIERCIALELERIAVHIGDTAALCTDVAYQLGQVVCEALRTTI
                     INTTQQWCGNRFGKGLIRPGGSHYPLSEDMATKIEKNLENTGTRFRKMTETLYSMPSV
                     LSRFDEIGTVTTQQALAVGAVGMAARTCNVPRDIRASHPFQYFKTYPVHPVVLETGDV
                     LARGMLRSMEVTESIKIIRELIATWRQKENKAKAPVYDYHLKPGSIAFSMTEGWRGEI
                     FHSAITNSKGQIVHYKVKDPSVHNWMALALAVRNQEISDFPVCNKSFNLSYCG"
     gene            complement(1520..2941)
                     /locus_tag="PKM34_08865"
     CDS             complement(1520..2941)
                     /locus_tag="PKM34_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ83741.1"
                     /translation="MTLTLFFILSAVMAILVFILKNRTITKNIIISYILLHIVFTAYC
                     WINLNSTELVYFTFDSAGVLMLAVLSLLLIPTVYHGFVYTKTDKTRNFNIYHTALIAL
                     VTSMTGAYLANGMTVLWIFVEATTLSVAALVYHDRTEKALEATWKYVFICSVGIAIAY
                     IGILLLSFIYGRGDAPILSFSSFLNLASIANPLYLKIAFVFVVVGFSTKMGLFPMHTV
                     TTDAHSVAPPPISALISTTLMNVGFLAIFRVYTLFSSTVILPFMNNVLILSGILSLLV
                     AAGYMLKAKHMKRMLAYSSLENMGLVAIALGIGGIGYYAAFLLIILHSLTKASLFFHM
                     GQFFRVLHTFRLDDCGKYMKLHPAGGLIMLTGLFGILAIPPSGLFISELMIFKGLVFN
                     NQWFLLIVTALLLCCIIYALSTRVMHIVFSDPRDIELQEPGIIKPAETISQFVLLGLV
                     FFMCFYQPPFLVEMINRSIVALP"
     gene            complement(2945..3559)
                     /locus_tag="PKM34_08870"
     CDS             complement(2945..3559)
                     /locus_tag="PKM34_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83742.1"
                     /translation="MVNYLIVLFAVTLIYISVAERFRIYAGLMGLQGFLLFGITLLEL
                     QEVNTTNLVIIASETVFFKGLVVPFLLFRIIKKTGVYKVHKLAMPGFYLLILTIIALI
                     LSLVLSKALVNPYINTEYMTIALFCLFTGMLLIVTHKYILSQVIGFLVIENAVFILSL
                     AVGNKNPMLINIGILLDIFVGVLILGMFALRLKPHTLELTRLKD"
BASE COUNT         1116 a          842 c          834 g          923 t
ORIGIN      
        1 tccgcaatag ctcaggttaa aactcttgtt gcaaaccggg aaatcggata tttcctggtt
       61 gcggacggcc agtgccagcg ccatccaatt atggacagaa gggtctttca ctttataatg
      121 aacaatttgt ccttttgaat tggtgatagc gctgtggaag atttctcccc tccagccttc
      181 ggtcatggaa aaagcgatac tgccgggctt gaggtgataa tcgtacaccg gggcttttgc
      241 cttattctct ttctgacgcc aggttgcgat cagttccctg atgattttaa tggattcagt
      301 gacttccata ctcctcagca taccgcgggc aagcacgtca ccggtttcaa gcacaacggg
      361 atgtaccgga tatgttttaa agtactggaa tgggtgcgag gcacggatat ccctgggtac
      421 attgcacgtt cgtgctgcca tgccgacagc cccgacagct agcgcctgct gcgttgtcac
      481 agttcctatc tcgtcgaagc gcgacagcac gctgggcatg gagtaaagag tttcggtcat
      541 tttccggaaa cgggtacccg tattttcaag attcttttcg attttagttg ccatatcctc
      601 ggatagcgga taatggctgc caccaggcct gatcaatcct ttcccgaaac ggttgccgca
      661 ccattgctgt gtagtattga tgatggtagt ccggagcgct tcgcatacga cctgcccgag
      721 ctggtaggcc acatcggtgc acaatgcagc cgtatcacct atatgtacgg caatgcgctc
      781 aagctcaagt gcaatgcaac gctcaatttg aaggtcctcc tcgatttgga tgccggcgag
      841 cgactcaata agctgggcat gagccagtgc atggccaaca gcggtatcac cggcgatgca
      901 ctcagacaag atacaccttt gcaacgggga atctttgtcg agatacaacc attcaattcc
      961 gcgatgctgg tagcccagat ggatctcgag atgcaaaacc ttctccccgt tacaaataaa
     1021 ccgaaaatga cccggttcaa tcaccccggc atggatcggt ccaactccca cttcgtgcag
     1081 ttcttcactt tctatctcaa agaaaggata attatccatc acctttgctt tgtcgtaccg
     1141 gtcaaatggg taccgtaccg gtttaagcca ggggtgaccc tcgaacgcaa cgccaaaggt
     1201 ctcatgaatt tcccgttcga aaatatgcat cgcgttgcac aagggagtta gcgatttaag
     1261 gatcagtccg tatttgttgt gcctgtgtga taaaatagta acgctttggt cgctgtcatc
     1321 gccgataaaa caataaaaaa ccagtttgcc ctcattctca atgggataat aagccaggca
     1381 atggtttgat tcctttttaa gaagttccgc cacaagatta taaaaaacct cataatcaga
     1441 ctttgatata tcttcaagcc tgcatggcac atcagtatta gctcttttaa tgtattctgg
     1501 ctgactcata aattcgctgt taaggcagag cgacaatgct ccggttgatc atttccacta
     1561 aaaatggcgg ttgataaaaa cacataaaga atacaagccc aagcaagaca aactggctga
     1621 tcgtttcagc gggttttatg ataccgggct cctgtaattc aatatcccgc ggatcagaaa
     1681 agacgatatg cataacacgg gtgcttaatg cataaatgat acaacaaagc agtaatgcag
     1741 tcacaattag caagaaccat tggttattga ataccagtcc tttaaagatc atcaattccg
     1801 aaataaatag tcctgacgga ggaattgcaa gaataccgaa taaaccggtc agcataatca
     1861 ggccgcctgc cggatggagc ttcatgtatt tgccgcagtc atccagcctg aaagtatgaa
     1921 gaacccggaa aaactggccc atatggaaga aaaggctcgc tttcgtcagc gaatgaagaa
     1981 tgatcagcag aaaggctgcg tagtaaccga tcccgccgat gccaagcgca atagcgacca
     2041 ggcccatgtt ttccaggctg gaataagcca gcatcctctt catatgttta gctttcagca
     2101 tgtaaccggc cgcaaccaaa agcgataaga tgccagaaag gatcaggaca ttattcataa
     2161 aaggtaaaat aactgtagaa gaaaaaagag tataaacccg gaaaatcgca aggaatccca
     2221 cattcatcag cgtggtggag atcaatgcgc tgatcggcgg gggagcaacg gaatgggcat
     2281 cggtcgtcac tgtatgcatt ggaaaaagcc ccattttggt actgaatcca accacaacaa
     2341 aaacaaaagc aattttaaga tagagcgggt tggcaatgct ggccaggttt aaaaaagagg
     2401 agaacgacag tattggcgca tcaccgcgtc cataaataaa gctcaggagc aatattccga
     2461 tataggcaat ggctatgcca accgaacaaa tgaatacata tttccatgtt gcttccagcg
     2521 ctttttccgt acggtcgtga taaaccaggg cagcgaccga caacgttgtc gcctcaacaa
     2581 agatccataa aacggtcatc ccatttgcca ggtaagcgcc tgtcatactg gttacaagag
     2641 caataagcgc cgtgtgataa atattgaagt tcctggtttt atcagtcttt gtataaacaa
     2701 aaccatgata aacagtggga atcagtagaa gggatagtac tgcgagcatt aagacacccg
     2761 cactgtcgaa tgtaaagtaa accagttcgg tggaatttaa attaatccag caataggctg
     2821 taaaaacaat atgtagcagg atataggaga taataatgtt tttagtaatg gtcctgtttt
     2881 tcaatataaa caccagaatc gccattactg ctgaaagaat aaaaaatagt gtaagagtca
     2941 tcttctaatc ttttaaccgg gttaattcga gggtatgggg tttcaaccgc agagcaaaca
     3001 tgcccagtat cagcacgccg acaaagatat cgagcaaaat gccgatgttg atcagcatag
     3061 gattcttatt tccaaccgcc agtgataaga tgaaaacggc attctcaata accaggaacc
     3121 cgatgacctg agataaaata tatttatggg ttacgataag caacatccct gtaaataagc
     3181 aaaagagcgc tatcgtcata tactcggtgt taatatatgg attaaccagg gcttttgaga
     3241 gcactaagct caagatcaac gcgataatcg taaggataag gaggtaaaat ccgggcatag
     3301 ccagtttatg aactttataa acccctgtct ttttgataat cctgaacaac aggaacggta
     3361 ccaccagtcc tttaaagaat acggtttcgg aagcgattat aaccaggttg gtcgtattga
     3421 cctcctgtag ttccagaagg gtaatcccga acagtaagaa tccctgcaga cccatcaggc
     3481 ctgcatagat cctgaaacgt tcggcaacgg aaatgtatat gagcgttaca gcaaacaata
     3541 cgatcaggta gtttaccata gtttaagtga ttagcttttc tgtcaatatt aaaacgacca
     3601 taaacgcaat cagtgctatg gccgacaaag tcaggatgaa tttcgggttt ttccccattt
     3661 tattgcgtgc tctgaatgat tccaggagcc cgatcatgac tgcaaataca acctg
//
LOCUS       DAOQXS010000312         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25801_length_3705_cov_2.994247, whole genome shotgun sequence.
ACCESSION   DAOQXS010000312 DAOQXS010000000
VERSION     DAOQXS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25801_length_3705_cov_2.994247"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..855
                     /locus_tag="PKM34_08875"
     CDS             19..855
                     /locus_tag="PKM34_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83743.1"
                     /translation="MKKFYILLIMMLSVSGFVFSQVSSQMDNPPAPVMASPESHVYRS
                     SGCNFLVFMDNLPWNSSAIQTILTANGESWVLQNSASMASTDFSMYDVIIIASDQIPS
                     FYDNFAANFAKFVTFVTNGGSLEVHAATCGWNSPCGYSVLLPGGVFTTEQYDDYNLVV
                     DAAHPIVAGVSSPFSGSYASHGYFSNLVAGTDIITETSSNNKPTTIQYVYGAGIVTAT
                     TCTYEFGYDFGQEAGEMLVNNLNYGCEHAIDAKVPVSDWAIAIGIFMILFAAVLRYRR
                     VF"
     gene            949..>3705
                     /locus_tag="PKM34_08880"
     CDS             949..>3705
                     /locus_tag="PKM34_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNQ83744.1"
                     /translation="MHKSYLLFSGFLFSFICQAQQIPEWEDPQVTGVNKVQPHATLIP
                     YPDERTAIENVKSKSPFYLLLNDKWKFNWVEKPFDRPAGFYNPDFDDSGWNTIPVPAN
                     WELLGYGVPIYVNISYEWTRNPNPPEVPHDYNPVGCYRHRFTLPENWKDKEIFIHFGA
                     VKSAFYLWINGKKVGYSEDSKTPAEFNITEYVKAGANILAMEVYRWSDGSYLECQDFW
                     RISGIERDIYLVARPPVYIRDFFVHTALTDDYRNGEFKLETDIGTNGGKPGGYTLKVK
                     LLTSDGKITVAAFSQEIKFSGKRESQTVFNQMIENPLKWTAETPDLYTLVLSVFNNKG
                     VCEESVSCKVGFKSYEMKNGQFLVNGIPVLIKGVNRHEHDPVTAHVISEESMLDDIRL
                     MKQFNINTVRTCHYPNDPRWYELCDQYGLYVIDEANIESHGMGYALERTLGNNPAFMK
                     AHLDRVEAMLERDKNHPSIIFWSMGNEAGDGVNFDTCYKWIKKRDPSRPIHYERAELR
                     HNTDVYCPMYPDIEYLEEYASQPQARPLIMCEYAHGMGNSTGNLRDYWDVIEKYPQLQ
                     GGCIWDWVDQGYEKKDQNGTAYFAYGGDYGPPGTPSDSNFCINGLVLPDRTPHPALYE
                     VKKVYQYIDVRAVDPDRGIISVKNNYDFIPLTGFDIHWKLKGDGAVMAFGTLEQPDIL
                     PREEKSYSIPLPEIKPEPGVEYFLDFSMTIREANGLIPEGHEVASEQLPLTLKADPLL
                     VNDKAFLEIEWSKDRKKVDITGVDFLVRFDTLAGQITRLEYNGRNYISQRPLPNFWRA
                     PIDNDFGNRMPKRCAVWKEASLERTVKSFAAKKAAQGIAVVSVIFELGKEKFQYRTNY
                     EIHSTGDIIITGEIKLDGRTTLPELPRFGMNFRIPAEFDHVKWFGRGPHENYQDRNTA
                     SFIGI"
BASE COUNT         1019 a          840 c          865 g          981 t
ORIGIN      
        1 aactttaaaa ctataactat gaaaaaattc tacattttat tgattatgat gctgtctgtc
       61 agcggctttg tgttcagcca ggtcagttca caaatggata atccgcctgc tccggtcatg
      121 gcctcaccgg aaagtcacgt ttatcgcagt tcaggatgta acttcctggt ttttatggat
      181 aatcttccat ggaatagcag tgccattcag acaattctga ccgccaatgg agaatcctgg
      241 gtgttgcaaa attctgcatc gatggcatca acagattttt ctatgtatga tgtgatcatc
      301 attgcttccg atcagatacc ttcattttat gataattttg cggctaattt tgcgaagttt
      361 gtaacttttg ttaccaacgg aggatcgctt gaggttcatg cagctacctg cggatggaat
      421 tctccatgcg gctactctgt tctgttacca ggaggtgttt ttaccacgga gcaatacgat
      481 gattataacc tcgttgttga cgctgcccat ccgattgtag ccggagtatc aagcccattt
      541 agtggtagtt atgcaagcca cggttacttc tcaaaccttg ttgccggcac ggatattata
      601 acagaaacct cttccaacaa taagcccacg accatacaat acgtttatgg agcgggaatc
      661 gtcactgcta ctacctgtac ttatgaattc ggctatgact ttggtcagga agcaggtgag
      721 atgctggtca acaacctgaa ctacggatgt gaacatgcca tcgacgctaa ggtgccggtt
      781 tccgactggg ccatcgccat cggtatcttc atgatccttt ttgccgctgt attgcgctac
      841 agaagagtat tctaataata gatatttaaa tatttgcaga gagtgtctcg tttaccgggg
      901 cactcttttt tttattttta tccaaaatat ccgcgataat tcatttttat gcataaaagt
      961 tatcttttat tctctggttt cctgttctca tttatctgcc aggcacagca aattccagag
     1021 tgggaagatc cccaggttac gggagtaaat aaagttcagc ctcatgccac tcttattccc
     1081 tatcctgatg agagaaccgc tatcgaaaat gtgaagtcaa agtccccttt ttatctcctt
     1141 ctgaatgata aatggaaatt caactgggtc gaaaaaccgt tcgatcgccc ggccggtttc
     1201 tacaatcctg attttgacga ttcgggctgg aatactatcc ctgtccctgc taattgggag
     1261 ttgctcggat acggtgtccc catctacgtt aatatatctt atgaatggac ccgtaatccc
     1321 aatccgcctg aagtaccaca cgactataat cccgtcggat gttaccgcca ccggtttact
     1381 ctccctgaaa actggaaaga taaagagata tttatccatt tcggggcggt gaaatctgct
     1441 ttttacctct ggatcaacgg aaaaaaggtc ggctacagcg aagatagcaa aacccctgct
     1501 gaatttaata ttacggaata tgtcaaggca ggcgcgaaca tcctggcgat ggaagtgtac
     1561 cgttggtccg acggatcata ccttgaatgc caggacttct ggcgtatcag cggcatagaa
     1621 agggacattt atcttgtcgc ccggcccccg gtttatatcc gcgacttttt cgtccataca
     1681 gcacttacgg atgactatcg gaacggtgaa ttcaaactcg aaacagacat tgggactaac
     1741 ggtggaaaac cgggcggtta cacattgaaa gtcaaactgt tgacatcaga cggcaaaatc
     1801 accgttgcag ctttttcaca ggaaatcaaa ttctctggta aaagagaatc acagaccgtc
     1861 tttaatcaaa tgattgaaaa tccattaaag tggaccgccg aaacaccgga tttatataca
     1921 ctggtgttgt ctgttttcaa taacaaaggc gtttgtgaag aatcggtcag ttgtaaagtg
     1981 ggttttaaat catatgagat gaaaaacgga cagttcctgg tcaacggtat cccggtattg
     2041 atcaaagggg ttaaccgcca tgaacacgat ccggtaacgg ctcatgtgat ttcggaagag
     2101 tcgatgcttg atgacatccg gttgatgaag cagttcaata tcaatacggt acgcacctgc
     2161 cattatccga atgatccccg ctggtatgaa ctatgtgacc aatacgggct ttatgtgatt
     2221 gatgaagcaa atatcgaatc acacgggatg ggatatgctc tggaacggac ccttggcaac
     2281 aacccggcat tcatgaaagc gcacctcgac agggtggaag ccatgctgga acgcgataaa
     2341 aaccatccgt ccataatttt ttggtcgatg ggtaatgaag ccggcgacgg tgttaatttc
     2401 gatacatgct ataaatggat taaaaagcgt gatccttccc ggccgataca ttatgaacgt
     2461 gcggaattgc gccataatac cgatgtgtac tgcccgatgt accccgatat tgaatatctt
     2521 gaagaatatg catctcaacc acaggcacgt ccgttgatca tgtgtgagta tgcccacggg
     2581 atgggcaaca gcaccggcaa ccttcgggat tactgggatg tgatcgaaaa gtatccgcaa
     2641 ctccaggggg gctgcatctg ggactgggtc gaccagggtt atgaaaagaa agaccaaaac
     2701 ggaacggcct attttgcata cggaggcgac tatggcccgc cgggaacgcc cagcgacagc
     2761 aatttctgca tcaacggcct cgtcttgccc gaccggacgc cccatccggc tctttacgag
     2821 gttaaaaaag tgtaccagta tatcgacgtt agagctgtag atcccgatag gggaattatt
     2881 tcagtgaaga ataattatga ctttattccc ctgacgggat tcgatatcca ttggaaactg
     2941 aaaggcgacg gagcggttat ggcattcggc accctggaac aacctgatat cctgccgcgt
     3001 gaagaaaaat cttattctat accgttgccg gaaatcaaac cagaacccgg cgttgagtat
     3061 ttccttgatt tcagcatgac catccgcgaa gccaatggcc tgatccccga aggtcatgaa
     3121 gtggcctcgg aacaactgcc tctgactttg aaggctgatc ctttgctggt aaatgataag
     3181 gcattccttg aaatcgaatg gagtaaagac agaaaaaagg tggatattac cggggtggat
     3241 ttccttgtaa gatttgatac cctggcaggc cagatcaccc ggctggaata taacggccgc
     3301 aattatatct cacaaagacc gctgccaaac ttctggcgtg cgccaatcga caatgacttt
     3361 ggtaaccgta tgcccaaacg gtgtgccgtc tggaaagagg ccagccttga acgaacggtt
     3421 aaatcgtttg cagccaaaaa agcagcacag ggtattgcag ttgtatcggt gatatttgaa
     3481 ctaggtaagg agaagttcca ataccggacg aattatgaaa ttcacagcac aggcgatatc
     3541 atcatcaccg gtgagattaa actggatgga agaacaaccc tgcccgagct tccccggttt
     3601 ggaatgaatt tcaggatccc ggcggaattt gaccatgtga agtggtttgg cagagggccg
     3661 cacgaaaatt accaggaccg caataccgct tcttttattg ggatt
//
LOCUS       DAOQXS010000313         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25846_length_3701_cov_3.583379, whole genome shotgun sequence.
ACCESSION   DAOQXS010000313 DAOQXS010000000
VERSION     DAOQXS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25846_length_3701_cov_3.583379"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1713
                     /gene="ppdK"
                     /locus_tag="PKM34_08885"
                     /pseudo
     CDS             1..1713
                     /gene="ppdK"
                     /locus_tag="PKM34_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
     gene            2024..3649
                     /gene="pckA"
                     /locus_tag="PKM34_08890"
     CDS             2024..3649
                     /gene="pckA"
                     /locus_tag="PKM34_08890"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNQ83745.1"
                     /translation="MDKVRKDLESYGVKDVAEIIHNPSYDYLYEAELDPVLEGYEKGY
                     LTESGAVNVLTGRFTGRSPKDKYIVLDEKTKDTVWWAAPNIRSDNKPIDHQTWEYCYK
                     LGAEQLSGRKLYVMDGYAGTNKDTRLNIRFVMEVAWQAHFVKNMFIRPTEEELENFKP
                     DFVVLNAAKTINPEWKNMKRTDGVPFNSEVFVMFNLTEGRAVIGGSWYGGEMKKGIFS
                     VMNYYLPLKGVAAMHCSANVGKAGDTAIFFGLSGTGKTTLSTDPKRALIGDDEHGWDD
                     EGIFNFEGGCYAKCINLDKESEPDIYQAIKRDALLENLVVDANGKIDYKDGSITENTR
                     VSYPIYHIDNIVKPVSKAGPAKKVIFLTADAYGVMPPVSILTPEQTQYHFLSGFTAKL
                     AGTEVGITTPQPTFSACFGAAFLLLHPTKYGEELVKRMEANGARAYLVNTGWNGTGKR
                     ISIKDTRKIIDAILDGSIDKAETKTIPVFNFKVPVALHDVDPNILDPRDTYQDKMEWY
                     KKAHLLGELFVKNFAQYTDNEHGKALVKAGPEV"
BASE COUNT         1051 a          884 c          965 g          801 t
ORIGIN      
        1 gtactttaca aagaattaga caagatccag caaaaactgg aagaccatta taaagacatg
       61 caggacctgg agttcaccat ccaggaaggc aagctgtggc tgctgcaaac ccgcaacgga
      121 aagcgtaccg gctccgccat ggtaaaaatt gccatggata tgctgaaaga gaaaatgatc
      181 gatacaaaaa ccgccctgtt gcggatcgaa cccaataagc tcgatgaact gctccaccct
      241 gtttttgata aagatgccat caaaaaagcc aaggtgctgg caaaaggact tcccgcctct
      301 ccgggcgccg caaccggtca gatggttttc tttgccgacg aagccgaaac ctgggctgct
      361 caaggaaaat ccaccattct tgtgcgtata gaaacgtctc ctgaagacct taaagggatg
      421 aacgttgcca atggcattct tacggcccgc ggggggatga cctcacacgc cgccgtcgtt
      481 gcccgcggaa tgggtaagtg ctgcgtctcc ggtgccggta gcattaagat cgattacaag
      541 agccgcacga tgaccatcga taaaaaagaa ttcaaggaag gcgactggat ctctctgaac
      601 ggatcgaccg gcgaggtgta cgaaggtaag attgccacac aggacccgga gctcagcggc
      661 gatttcggcg acctgatgga gctggccgac cgttttgccc gtatgcatgt ccgcaccaat
      721 gccgacaccc cgcatgatgc acaggttgcc cgaaacttcg gcgccaaggg catcggcctc
      781 tgccgcaccg agcacatgtt cttcgaaggc gaccggatca tcgccatgcg tgaaatgatc
      841 ctcgctgatg atgaagcagg gcgccggaaa gccctcgaca agctgcttcc gatccaacgc
      901 ggcgatttcc agggtatcct ggaggctatg cacgacctgc cggtgactat ccgcctgctc
      961 gacccgccac tgcatgagtt tgtaccgcac gaagatgaga accagaaggt tatggccaag
     1021 cagatgggcg tcagcgtcaa agcgatcaag cagaaagtgg atgaactgca cgaaatgaac
     1081 ccgatgctgg gtcaccgcgg atgccgcctt ggcaacacct atcctgaaat ctccgagatg
     1141 caggccaggg ctattatcga agcggccctc aacctgaaga aaaaaggggt gaacgccaag
     1201 ccggagatca tgatcccatt gacgggaaca ctgaaagaga tgaagatgca ggaagatatc
     1261 gtgaggtcga cggccgaaaa ggttttcgcc gaaaggaaag accggatcga ttacctggtc
     1321 ggaacgatga tcgaagtgcc gcgggcagca gtgacagccg atgaaatcgc cgaatcggcg
     1381 gagttcttct ccttcggcac gaacgacctg acccaaatga cattcggcta ttcgcgcgac
     1441 gacgccggaa aattcctccc ggtgtacctc aagaaaggca tcctgaagca cgatccgttc
     1501 caggtacttg accaggacgg tgtcggcaaa ctgatgaaga ttgcggtaaa gaaaggccgt
     1561 aagacccgcc cgaacattaa gatcggtatc tgtggcgagc atggcggcga gccatcctcc
     1621 gtagaattct gcgattccct tggcttcaac tatgtttcct gttcaccttt ccgtgttcca
     1681 atcgcccgtc tggccgctgc tcaagccgtg atccgcgaga aactggcaaa gaaatccgac
     1741 aagaaaggaa agaaggaaga ggaaaaatct tctaaaaaag gaaagaaagc gaaaaaatag
     1801 tagtgaatct caatcataag tttgaagagg atgtcttaat aaatgagcat cctctttttt
     1861 attgattgtc tgccggctac tggctttttt cagtttcggt tcctttggtt ccggttaact
     1921 gcgtaagaat acatacacca aaataccggt ataaaaagta acaggaatat ttcctaattt
     1981 tgcctgcttt attaacctta taatatctga agaaatggct aatctggata aggtcaggaa
     2041 agacctcgaa tcgtatggtg ttaaagatgt tgcggaaatt attcataatc cttcctatga
     2101 ttacttgtat gaagcggaac ttgatcctgt tctggaaggc tatgaaaaag gttacctgac
     2161 ggaatcaggc gctgtgaatg tactgacggg aagatttacc ggacgctcgc ctaaagataa
     2221 gtatatcgta ctggatgaaa agacgaaaga tacggtttgg tgggccgcgc ctaacatccg
     2281 ttccgataat aaacccattg atcaccagac atgggaatac tgctataaac tgggcgcaga
     2341 gcagctttca ggaagaaagc tttacgtaat ggatggttat gctggaacta acaaagatac
     2401 ccggctgaat atccggtttg tcatggaggt agcctggcag gcccattttg tgaaaaatat
     2461 gtttattcgc ccgactgagg aggaactgga gaattttaag cccgatttcg tagtcttaaa
     2521 tgcagccaaa acgatcaacc cggaatggaa gaacatgaag cgtaccgatg gtgttccttt
     2581 caattcggaa gtttttgtga tgttcaacct gacggaagga agagcggtga tcgggggaag
     2641 ctggtatggt ggcgaaatga agaagggtat tttttcggtg atgaactatt atctgcccct
     2701 gaagggcgtt gcggccatgc actgctcggc caatgtgggc aaagccggtg atacggccat
     2761 tttcttcgga ctttcgggta cgggaaaaac gaccctttcc accgatccga aacgggcact
     2821 gatcggcgat gacgagcacg gatgggatga tgagggaatt ttcaatttcg agggcggatg
     2881 ttatgcaaaa tgtatcaacc tcgataaaga gagcgagccc gatatctacc aggcaataaa
     2941 aagggatgca ctgctggaaa acctggtggt ggatgccaat ggaaagatcg actataaaga
     3001 cggctccatc acggaaaata cgcgtgtctc ctaccctatt taccatatcg acaacatcgt
     3061 caaaccggtg tcgaaagcgg gcccggcaaa gaaagtcatt ttcctgacgg ctgatgccta
     3121 tggggtcatg cctcctgtct ccatcctgac acctgaacag acccaatatc acttcctttc
     3181 gggatttacg gcaaagctgg ccggcacaga agttggcatt acaacaccgc aaccgacttt
     3241 ctcagcctgt ttcggcgcag cattcctact tcttcacccg acaaaatatg gcgaagagct
     3301 cgtgaagcgc atggaagcca acggagccag ggcctacctg gtaaataccg gctggaatgg
     3361 aaccgggaag cgtatctcca ttaaggatac gcgaaaaatc atcgatgcga tcctcgatgg
     3421 ctcaatcgac aaagcggaaa caaaaacgat cccggtattc aactttaaag tcccggttgc
     3481 actgcatgat gttgatccga atatcctgga tccgcgagac acgtaccagg acaagatgga
     3541 atggtacaag aaagcccatc tattgggaga acttttcgtt aagaactttg cgcagtacac
     3601 tgacaatgaa cacggtaaag cgctggtcaa ggcagggccg gaggtttagt tcaaagttca
     3661 aagttcaaag ttcaaagttc aaagttcaaa gttcaaagtt c
//
LOCUS       DAOQXS010000314         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25885_length_3697_cov_3.299561, whole genome shotgun sequence.
ACCESSION   DAOQXS010000314 DAOQXS010000000
VERSION     DAOQXS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25885_length_3697_cov_3.299561"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2962)
                     /locus_tag="PKM34_08895"
     CDS             complement(<1..2962)
                     /locus_tag="PKM34_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83746.1"
                     /translation="MSLRRVVLSFLALLASLSAISQGLSCADSDPFCTGTIYEFPAGT
                     TGAAEAGPYYGCLSTQPAPAWYHMKIGNPGPITIYMHSEPQEDIDFACWGPYTDPTSA
                     CPYGLTQSKMVDCSYSTSWQEYCDIPNGQTGQYYILLITNYSQDPCNIIFSQTAGSGT
                     TDCSILPPLVWSDSPLCVGETLHLMAETISNATYSWTGPAGFSSTQQNPEIPNVTLAM
                     AGDYSCIITVFSQSSPPAVTTVVINDLPDASLLNSSQTTCPGDTVMIALQLTGAAPFT
                     LTCFNGTIYTTYEDLYGPVDTIYLAPPGPVTYTFTQVDDTNCTKYLSGTVFTVYNFPP
                     ATGIMSGGETICSGDTSELVFNLTGTPPWEITYLTNGMNPVTVVAEYTPFSVFVAPQV
                     TTVYQFTQLSDLHCEGSASGQVQVSVDYPTGTLSGDNTICAGSGAILLFILSGYPPWT
                     ITYTENGGNGQEVTTYSSPYALTVYPVENTLYEFTLLSDNYCTGSASGQAMITINQPT
                     GVLSGESTICAGESSEITFDLGGVAPWHIIFEQNGSNPQELNANTTPFPVSVTPLLTS
                     IYTFTYFEDGACEGIPSGSAQVIVNPLPLAQAGNDKTIPNGTSTTLNGSVSGGSGSYA
                     VQWEPASKLVNPDILQPQTVNLFESTLFTLTITDDEGGCMGQDEALVTITGGTLSCFS
                     LSAPSVICRGETSQLQVIASGGSGTYTYLWQSDPPGFSSDLAVLQVNPEVTTTYTVTV
                     NDGFNVSQSQATVQVDPLPVPFAGDNQVIVYGTPTQLHGTASQGSGNYSFHWEPAYKL
                     IDPDSENPHTVNLYETTVFTLSVTDLETGCPCEAVDAVSVMINGSALSVNPTALLNEI
                     CLGDTVQLFSSSGGGTGIYSHTWTSLPEGFNSSEADPLVVPGNSMTYLVDVWDGYNWA
                     SGSVEVKVNPNPVVHMGNDTTVCVFDTITLDAGPDGASYLWFNGATGRYISISSTGIG
                     YEARQVSVEVASDKG"
     gene            complement(2999..3517)
                     /locus_tag="PKM34_08900"
     CDS             complement(2999..3517)
                     /locus_tag="PKM34_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83747.1"
                     /translation="MKTVLLCLALSVSSFWAFSQAQNTEELIRKFGKNSLENGATPYS
                     YCFGVNPKCHASGCSEVTVKTPKDSDVMVTIKTGDTVVRHAYIRSGNSYTFEMPNGTY
                     QVFFYYGTGWNPGKVMKSTGECEVKGGFVTDEYFGKDDPQVLDNTILEYELILQKNGN
                     FSTRPSDAGEAF"
BASE COUNT          984 a          880 c          939 g          894 t
ORIGIN      
        1 atcctttatc ggaggctact tcaacggaaa cctggcgtgc ttcatacccg ataccggtgg
       61 agctgatgct gatatagcgt ccggtcgccc cgttaaacca caaataggaa gccccgtcgg
      121 gaccggcatc aagcgttatg gtatcaaaaa cgcacaccgt agtgtcatta cccatatgca
      181 caaccgggtt cggattaacc ttcacttcca ccgatcctga tgcccagtta tagccatccc
      241 atacgtctac taaataggtc atactgtttc caggtacgac tagcgggtcg gcttccgacg
      301 agttaaaccc ttcgggtagc gatgtccagg tatggctata aatgcctgtt ccacctcctg
      361 aggaagagaa aagctgaacg gtgtcaccca ggcagatttc attcaggagt gctgtcgggt
      421 ttacacttag agcagaaccg ttgatcatca cggaaaccgc atccacagcc tcacacgggc
      481 atccggtctc caggtcggtc accgacaggg taaatacagt ggtctcatac aaattgaccg
      541 tatgcgggtt ctctgagtcg ggatcaatca gtttgtaagc cggttcccag tgaaatgaat
      601 aatttcctga gccctggctg gccgtaccgt gcaactgtgt gggcgttccg tatacgatga
      661 cctggttatc accggcaaaa ggaacgggta aaggatcaac ctgcacggta gcctgggatt
      721 gtgagacatt aaacccatcg tttacagtca cggtataagt ggttgtcacc tccggattaa
      781 cctgcaaaac ggccagatct gaagaaaaac cgggaggatc ggattgccac aggtaagtgt
      841 aagtacccga gccgccggaa gcaataacct gcaactggct tgtttcgccg cggcaaatca
      901 cgctgggcgc tgataaagag aaacaactca gcgtacctcc ggtgatagtc accagagcct
      961 catcctgtcc catgcaacct ccttcatcat cggtaatggt tagagtaaaa agggtggatt
     1021 cgaaaagatt aacggtctga ggctgaagaa tatcaggatt aaccagctta gaagccggtt
     1081 cccattgaac ggcataacta ccggaacctc ccgatacgga tccatttaaa gttgtggatg
     1141 tgccattggg aatagtctta tcattgcctg cctgcgctaa cggcaacgga ttgacaataa
     1201 cctgtgcaga gcctgaagga atcccctcac aagccccatc ttcaaaatag gtaaatgtat
     1261 aaatggaggt taataaagga gtgaccgata cagggaaggg agtggtattg gcattgagtt
     1321 cctgcggatt acttccgttc tgttcgaaaa taatgtgcca cggggcgact cctcccaggt
     1381 caaaggttat ttcagaggac tccccggcac agatcgtaga ttcaccgctc aatacacctg
     1441 taggctggtt aatcgtgatc atggcctggc cgctggccga gccggtacaa tagttatctg
     1501 acagcaaagt aaattcatat aaggtatttt ctaccgggta aaccgtaaga gcataaggag
     1561 atgagtaagt cgttacctcc tgtccgtttc ctccattttc cgtataagta atagtccagg
     1621 gtggatatcc tgaaaggatg aacagtaaaa tggctcccga tccggcgcat atagtattat
     1681 cgccactcag ggttcctgtt ggataatcca ccgaaacctg cacctgcccg gatgcggagc
     1741 cctcacaatg cagatctgac aactgtgtga actggtaaac ggttgtaacc tggggcgcta
     1801 caaaaactga gaaaggagta tattccgcca caaccgtaac cgggttcatc ccattggtaa
     1861 ggtaagtaat ttcccaggga ggcgttcctg taagattaaa taccagctcg gaagtatcgc
     1921 cgctacaaat cgtttcgcct cccgacatta ttcctgttgc cggaggaaaa ttatatacgg
     1981 tgaagaccgt tcccgacagg tacttcgtgc aattagtatc gtccacttgt gtgaacgtat
     2041 aagtgacagg gccgggtggc gccagataaa tggtgtctac cggaccgtaa aggtcctcgt
     2101 aggttgtata tatggtcccg ttaaaacatg taagagtaaa aggagcggcg ccggtaagct
     2161 gcagagctat catgacagta tcgccgggac aggtagtctg gctgctattt agcagggatg
     2221 catccggcag atcgttgatc acgacagttg ttacggccgg cgggctagac tggctgaaca
     2281 cagtgataat gcaggaataa tctcctgcca tggctaatgt tacattgggg atctccggat
     2341 tttgttgtgt cgagctgaat ccagccggcc cggtccatga ataggtggca ttggaaatgg
     2401 tctctgccat gaggtgtagt gtttctccaa cgcacaaagg gctgtcgctc cacaccagtg
     2461 gtggcaagat ggaacaatcg gtggtgccgg atcctgcagt ctgtgagaag atgatattgc
     2521 atggatcttg tgaataattc gtgatcagga gaatataata ctgtcctgtc tggccatttg
     2581 ggatatcgca gtattcctgc cagctcgtgg aataggaaca atcgaccatt ttactctgcg
     2641 tcagtccata tgggcaggcc gaggtcggat cggtataggg tccccagcat gcaaagtcta
     2701 tatcttcctg aggctcactg tgcatgtata tcgtaatcgg cccgggattg cctattttca
     2761 tatgatacca tgccggggca ggttgtgtgg aaaggcatcc gtaataggga ccggcttcag
     2821 cagcgccggt ggtgccggcg ggaaattcat agatcgttcc tgtgcagaaa ggatctgagt
     2881 cagcacagct caggccctgt gaaatagctg aaagagaagc caataaagct aagaatgaaa
     2941 ggacgaccct tctgagtgac atgaccaatt ttaattttca ataaaaataa gcaaatgatc
     3001 aaaacgcttc accggcatcg ctgggccgtg tcgaaaaatt tccattcttc tgaagaatga
     3061 gttcgtattc cagaatggta ttgtcaagaa cctgaggatc atctttaccg aaatattcat
     3121 cggttacaaa tcctcctttc acttcacatt ccccggtgct tttcatcacc ttgccgggat
     3181 tccatcctgt accgtaataa aagaaaacct ggtaagtgcc attcggcatc tcgaaagtgt
     3241 aagagttgcc cgaacggata taggcgtgcc tgaccaccgt atcgccggtt tttatcgtaa
     3301 ccatcacatc gctgtctttc ggggttttaa cggtaacctc ggagcagccc gatgcgtggc
     3361 atttcggatt aacgccgaaa caataggaat aaggcgtagc gccattttcc agtgaattct
     3421 tcccgaattt ccggataagt tcttccgtat tctgggcctg gctgaaagcc cagaaagacg
     3481 aaaccgacag tgcaaggcat aagagaactg ttttcatttt tagtctggct tttgatgaga
     3541 tgactaaatt actaaaaatg agaagacaaa aaaaacctct ggagagaaat ctccaaaggt
     3601 tgggctatgc ggtccggaca gcttgtcccg agtgctcggg aggactcgaa cccgcgacct
     3661 tcccgaggac tcgggacagg cattctaacc aagtgaa
//
LOCUS       DAOQXS010000315         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_25903_length_3694_cov_3.696070, whole genome shotgun sequence.
ACCESSION   DAOQXS010000315 DAOQXS010000000
VERSION     DAOQXS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25903_length_3694_cov_3.696070"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1428
                     /locus_tag="PKM34_08905"
     CDS             124..1428
                     /locus_tag="PKM34_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83748.1"
                     /translation="MLEHIKEIILDAQSGPLFTGTPRQTGLACIPGKASIIIGVRRSG
                     KSTVLNQEVDKWLSKGVLKENIFYINFFDDRLSGLKTIGLDIVLEAYYSLYPGKKNLE
                     TVYCFFDEIQVIPEWESFIDRILRTENVVVYLSGSSAKMLSKEMATQMRGRALAWELF
                     PFSFKEYTGHQNLKNDLPLNTQQRLFIENAFGEYWETGGFPEVAGMEKHLRIKIHQEY
                     YNSILFRDLIDRYDIPHPRALNDLSRHLLANVASMYSINSLTGMLKSLGYGVPKSVIA
                     EYISWFEDAYFLFTVRLYDASYRRSNANSKKIYCIDHALVRSITPGILTNSGHLLENI
                     VFLALRRVTAEIWYYRTTGNREVDFIYRNKIGEPVLIQVCESILIPGTRDREIKALRA
                     AMQELRITKGYIVTRNETEQITYPQGIIAVMPAWKFLLESWE"
     gene            complement(1468..2091)
                     /locus_tag="PKM34_08910"
     CDS             complement(1468..2091)
                     /locus_tag="PKM34_08910"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HNQ83749.1"
                     /translation="MKTLSIILVLLINGISSFSQSQIITAKDLPKLAAMMTGQFSSEE
                     QAAADKDFLHIRLSMTPIMPENKDGYWLYVEQAMASAMDKPYRQRVYRLSIADDTTIV
                     SKVFELKNPLAYTGGHEMPEKLAVITPDSLIDRQGCSIFLHKTGKKRFEGSTPGKECL
                     SSLRGATYATSEVVITPKQMVSWDRGWDREDKQVWGSEKGGYVFLKR"
     gene            2353..>3694
                     /gene="sufB"
                     /locus_tag="PKM34_08915"
     CDS             2353..>3694
                     /gene="sufB"
                     /locus_tag="PKM34_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNQ83750.1"
                     /translation="MPDTNEILDNLTKSEYKYGFVSDIDTETAPVGLDENTIRYISSR
                     KEEPEWMLEYRLKAYRHWLTMKEPEWAHISYPPINYQDIIYYAAPKKKQFTSLDEVDP
                     ELLATFDKLGIALEEQKRLTGVAVDAVIDSVSVKTTFQETLAKHGIIFCSFSEAVKNH
                     PDLVKKYMGSVVPYTDNYFAALNSAVFSDGSFCYIPKGVRSPIELSTYFRINAANTGQ
                     FERTLIVAEEGGYVSYMEGCTAPMRDENQLHAAVVEIMAMDNAEVKYSTVQNWYPGDK
                     EGKGGIYNFVTKRGLCKGANSKISWTQVETGSAITWKYPSCILMGENSIGEFYSVAVT
                     NNRQQADTGTKMIHIGKNTKSTIISKGISAGFSNNSYRGQVRITARAENARNFSQCDS
                     LLLGDKCGAHTFPYIDVDNRTAIVEHEATTSKISEDQLFYCQQRGIDMEKAISLI"
BASE COUNT          990 a          882 c          928 g          894 t
ORIGIN      
        1 ccccatacct gcttgtcttc cctgtcccag gtgattatta tttaacttat atttgtaatc
       61 ggtaattatt attactactt ttgctacaaa gtagatataa tattttcctg tttgatgatt
      121 gccatgttag aacatataaa ggaaatcatt ctggatgccc aatcaggtcc gttatttaca
      181 ggaacgccga ggcaaaccgg gttggcttgc ataccaggca aagcttctat tattatcggg
      241 gtcaggcggt ctggcaaatc tacagttcta aaccaggaag ttgacaaatg gttgtcaaaa
      301 ggtgtattga aagaaaatat tttctacatc aatttttttg acgaccggct atcggggctg
      361 aaaaccattg gcctggatat tgttctggaa gcctattatt cgctttatcc cggtaaaaag
      421 aacctggaga ccgtatactg tttttttgat gagatacagg taatccccga atgggagtca
      481 ttcattgaca ggatattaag aactgagaac gtggttgttt acctttccgg ttcatcagca
      541 aaaatgctat cgaaagaaat ggcgacacaa atgcggggaa gggcgctggc ctgggagctc
      601 tttccctttt cattcaagga atacacgggg catcagaatc tgaaaaatga tcttccgctg
      661 aacacacaac aacggctttt catcgaaaat gcatttggtg agtactggga aaccgggggc
      721 tttcctgaag tggcaggaat ggaaaagcat ctccggatta agatccacca ggaatactat
      781 aattccatcc tttttcgcga tctcatcgac cgatacgaca ttccgcatcc cagggcacta
      841 aacgaccttt cccgccattt gctggcaaat gtcgcatcca tgtatagcat caacagtttg
      901 acagggatgt taaaatcact gggatacgga gtgccaaaga gtgtcattgc ggaatacata
      961 agctggtttg aagatgccta ttttcttttc acagtaagat tatatgatgc ttcgtaccgt
     1021 cgttcgaatg caaattcaaa aaagatatat tgcatcgacc atgctctggt gagatcaata
     1081 acaccgggga ttttgaccaa cagtggccac ttactggaaa atattgtctt ccttgcgctc
     1141 agaagagtaa ccgccgagat atggtattac aggacgaccg gtaaccggga agtggatttt
     1201 atatatcgga ataagattgg tgaaccggtg ctgatccagg tgtgcgaatc gattttgatt
     1261 cccggcaccc gcgacaggga aataaaggca ctgagggctg ccatgcaaga gctcagaatt
     1321 acgaaaggat atattgtcac gcggaatgaa acggaacaga ttacataccc gcagggaatc
     1381 atcgcagtta tgccagcctg gaagtttctg ctcgaatcat gggaatagtt ataatttcat
     1441 atcctggatt cacatttgcc cggtgcacta tctcttgagg aagacatatc cgcctttctc
     1501 cgatccccag acctgcttgt cttccctgtc ccagccgcgg tcccaggaaa ccatttgttt
     1561 aggcgtgata acgacttctg aagtggcata ggttgcgccc cgcagcgaac tcaggcactc
     1621 tttgcccggg gtggaacctt caaagcgttt tttccccgtt ttgtgcagga aaatgctgca
     1681 tccctgccgg tcgatcagtg aatccggggt aataacagcc agtttctccg gcatctcatg
     1741 tccgccggtg taggccaacg gattcttcag ctcaaagact ttgctgacaa tcgtcgtgtc
     1801 gtccgcaatg ctcaggcggt agacccgctg gcggtagggt ttatccatcg ccgaggccat
     1861 cgcctgttcg acgtaaagcc agtagccatc cttgttctca ggcatgatgg gggtcatgct
     1921 cagccggata tggaggaagt ccttatctgc ggcggcctgc tcttcgctgc tgaactgccc
     1981 ggtcatcatg gcggcgagct tcggaaggtc tttggcggtg ataatttgtg actgggagaa
     2041 ggatgaaatc ccgtttatta ataacaccag gatgatacta agcgttttca tagacttatt
     2101 tttatagttt acaaaactat gtttttaagc ttatcaatag cacacggatg acacggatgc
     2161 gacggattaa cgcggattta tttcggattt tctattcact atgcacgagg gcgaaagaat
     2221 tgatgaaacc tggaacctgg aacattggaa ccctggaaca attctccccc ttctttgttt
     2281 catatgttac agtccaatcc gatgagaatg gttacttttg caatctcaat ttagattaaa
     2341 tcactgaagg cgatgccgga tacgaatgaa atactcgaca atctgaccaa aagcgaatac
     2401 aaatacggat tcgtatcgga tatcgatacg gagacggcgc cggtcggact ggacgagaac
     2461 acaatacgat atatctcttc gcgcaaggaa gaaccggagt ggatgctcga ataccgcctg
     2521 aaagcttacc gccactggct gacgatgaag gagccggagt gggcgcatat ctcctacccg
     2581 ccaatcaatt accaggatat catttactac gccgcgccga aaaagaagca gttcaccagc
     2641 ctcgatgagg tcgatcccga actgctggcc actttcgaca aactggggat tgccctggaa
     2701 gagcagaaaa gactgacggg cgtagccgtg gatgcggtga tcgacagcgt atcggtaaag
     2761 acgaccttcc aggagacgct ggcaaagcac gggatcatct tttgctcgtt cagcgaagcg
     2821 gtgaagaacc accccgacct ggtcaaaaaa tacatgggct cggtagtgcc ctacaccgac
     2881 aattactttg cggctttgaa ctcggccgtt ttcagcgacg gctcattctg ctacatccct
     2941 aaaggtgtcc gcagcccgat cgaactgtcg acctacttcc gcatcaacgc cgccaatacc
     3001 ggccagttcg aaaggaccct catagtggct gaagaaggcg gctatgtcag ttatatggaa
     3061 ggctgcaccg ccccgatgcg ggatgagaac cagctccatg ccgccgtggt ggagatcatg
     3121 gcgatggaca atgcagaggt gaaatattcg acggtccaga actggtaccc gggcgacaag
     3181 gaagggaaag gaggcattta caactttgtg accaaacgcg gtctttgcaa aggcgccaac
     3241 tcgaagatat cctggaccca ggtggagacc ggctcggcga tcacctggaa atacccgagc
     3301 tgcatcctga tgggcgagaa cagcatcggc gaattttact cggtagccgt taccaacaac
     3361 cggcagcagg ccgatacggg gacgaagatg atccatatcg ggaagaacac caaaagcacg
     3421 atcatttcca aggggatctc ggccgggttc agcaacaaca gctaccgcgg ccaggtgagg
     3481 ataacggcga gggcggaaaa tgccaggaac ttctcacagt gcgattcgct tctgctgggc
     3541 gacaaatgcg gggcgcacac gtttccctac atcgatgtgg ataaccgcac tgccatcgtc
     3601 gaacatgagg caaccacgtc gaaaatatcg gaagaccagc ttttctactg ccagcagcgg
     3661 ggtatcgaca tggaaaaggc tatcagcctg atcg
//
LOCUS       DAOQXS010000316         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26042_length_3680_cov_3.798345, whole genome shotgun sequence.
ACCESSION   DAOQXS010000316 DAOQXS010000000
VERSION     DAOQXS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26042_length_3680_cov_3.798345"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PKM34_08920"
     CDS             complement(<1..1716)
                     /locus_tag="PKM34_08920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ83751.1"
                     /translation="MESSILNQLNIIRSGERKKVLDQALALINNKGKDFEKPPVELIN
                     QLNRLSADIGMNNDFRRALENQDKLSNLLIKLYEGADPDLVQILPSTNDPAEFHRKMK
                     AYLPEQPVFKRLDPFFFLNKDVVVNKEMISVIVLNENGAPYLDDFFNSFAKFCSFNDY
                     RITMFDRASADNSIEIASSWCDRIPLEIIRFDERLTASYLYNTAVDNSSSQYILFVAN
                     SISFNQDILQKFLETIQKDESTGITGASLLLKDISGKSQEMELFGSILFRIEDIPGVP
                     DIPFPTINTRLLLKTGLDKHLKHYRKEGAVFVPPGLSMKGHKPFPASHTRKTGDEPVM
                     AVTGHALFCRRQDLIASGGFDLNFTDGFEEADLCLKFARRLKKKTILSHEIRVEMNNE
                     DSLKTMGGKFAIVNRYNLGTLINNHGGYLKRKYPADNTEPENQHIRIAIKTPAFDDER
                     TLMWGDYHFASSLKNAFVRKGYQARVDLHNFWYDNGYMTDDVVIVLRGTKRYHTRGSQ
                     VNILWNISHPEQVTEEEYKDYDYVFSASTFFADELKKKYGIMAENLLQCTDAGLFYPD
                     KENISE"
     gene            2092..>3680
                     /locus_tag="PKM34_08925"
     CDS             2092..>3680
                     /locus_tag="PKM34_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83752.1"
                     /translation="MAKSHEDDLSRKEAAWELALYHSVRKTKEDAKGCMKFLSSSTDE
                     NADMSRTIQAAIIRSECHNLLGEKHKAAEILITAIEKCPSPELLLAYANLAGDLKTKV
                     ECFNKAFMMSGLGPGLKESYSLLPYFFRIRSLEVIPEGIHADVARPLVSIIIPTYNCS
                     DTMGYVMDSVLSQTWKNIEVIVADDCSTDQTAGIVRGYLQKDKRVKFIRTEANSGLFV
                     AMNKALQIAKGEFVTCQDQCEWAHPEKISIQAGHLVGNPDYTANISRRIKVNSNLEPL
                     RAEHSGLFIQNNIRSLMFRRQRVMTSLGFWDSVRAGADEEMLFRLRRKFSDKRICYLE
                     TGPLAFLLEETSSFAKKRNPDVSPGALREYMESFKSFLAKNDSPSYEFPLKARRYPVP
                     EPFWPVAEKKTNGRRHFDVLLASEFRLPGGTTTSNVEQIKAQRAAGLKTGLVHLKRYD
                     INTSEPIRDKIRAEIDGDLVQMVVPGEKVSCDLLVVRHPPVLKDFQLFVPDIQAGHLL
                     VVINTSPLREYSIQGERVYDFR"
BASE COUNT         1016 a          799 c          847 g         1018 t
ORIGIN      
        1 ctccgatatg ttttccttgt cgggataaaa cagccctgca tcggtacact ggagaagatt
       61 ttccgccatt atcccgtact tctttttcaa ttcatccgcg aaaaaggttg aagcggaaaa
      121 cacgtaatca tagtctttgt actcttcttc ggtgacctgc tccggatggc tgatattcca
      181 taatatattg acctgggagc cacgcgtatg gtatcttttg gtccctctta aaactattac
      241 cacatcgtcg gtcatgtatc cgttgtcgta ccagaagtta tgcagatcga ctcgggcctg
      301 gtagcccttc cggacaaagg catttttaag cgaactggca aagtgataat cgccccacat
      361 taaggtccgt tcatcatcaa aagcgggtgt ttttatcgca atgcgtatgt gttgattttc
      421 cggctcagtg ttatctgctg gatattttct tttcaggtac ccgccatggt tattgatcaa
      481 tgtgccgagg ttatacctgt tcacaattgc aaattttcct cccatggttt tcaatgaatc
      541 ctcattgttc atttcaaccc ggatttcatg ggagagaatt gtcttctttt ttagcctcct
      601 ggcaaatttc aggcacaggt ctgcctcttc aaacccatcc gtaaaattca ggtcgaaacc
      661 gcctgatgcg atcagatcct gccgcctgca gaacagggca tggcctgtca cagccatcac
      721 cggttcatct cctgttttcc gagtgtgact cgccggaaaa ggtttatgac ctttcatcga
      781 caaaccgggt ggaacaaaaa ccgccccttc tttcctgtaa tgtttcaggt gcttatcaag
      841 gccggttttt aagagcaaac gggtattgat cgttggaaag ggaatatcag gcacaccggg
      901 aatatcttca atgcggaaca ggatactgcc gaacagttcc atttcctggg attttccgga
      961 gatatctttc aataacagac tggcgccggt aatgccggtc gattcatctt tttgtattgt
     1021 ttccaggaat ttttggagaa tatcctgatt gaatgagatg gagttagcaa cgaaaagaat
     1081 gtactgcgat gaactgttgt caacagcggt attataaaga taagaagccg ttaatcgctc
     1141 atcaaaccgg atgatctcca atgggatcct gtcgcaccag gaagaggcaa tttcaatgct
     1201 gttatctgcc gaagccctgt cgaacatggt gatccggtaa tcattaaagg aacagaattt
     1261 tgcgaatgaa ttaaaaaaat catcaaggta tggcgccccg ttttcattga ggacaattac
     1321 gctgatcatt tccttgttaa cgacgacatc tttattcagg aagaagaaag gatccaatct
     1381 tttaaaaacc ggttgttcag ggagataagc cttcattttc ctgtgaaatt ctgcaggatc
     1441 atttgtcgat ggcagaatct gaaccaggtc cggatcggcg ccttcatata attttatcag
     1501 cagattgctc agtttatcct ggttctccag tgcacgcctg aaatcattgt tcattccaat
     1561 atccgccgac agcctgttca gttggtttat cagctcaacc ggcggctttt caaagtcctt
     1621 acctttgtta tttatcagcg caagagcctg gtcaagaact tttttccgct ccccggacct
     1681 gatgatattt aactgattta aaatagaaga ctccataact aattaccccg tacgctgttt
     1741 atctgtctgt tataaaaaca tcgaaggtaa ggaaaccaga attaaaaatt tgtaccttgc
     1801 catatattat actcattata tttgatcgaa atggtgaatc taaaactgct tttgcgatca
     1861 ctaaaatacc aatttccggg aaatttaagg ctgataaaac tgccatcctt tcccgatata
     1921 tccgctaacc ggttgccagg acccgacaaa gaaccgaaag taatgaattc ggatgagttt
     1981 agtcctgacg atcttgaaat aattaagcgg aataaatggc gaattgcaaa tattgccagc
     2041 aaattaatga ccgatggttt tatagataga gcctatcctg aactgaatga aatggcaaaa
     2101 tcccatgagg atgatctctc gcgcaaggaa gctgcatggg aacttgcttt atatcattcc
     2161 gttcgtaaaa cgaaggagga tgcaaaagga tgcatgaagt tcttatcatc atccactgat
     2221 gaaaatgcgg atatgtccag gaccatccag gcagccatca tacggtcaga atgccataat
     2281 ttactgggag aaaagcataa agccgcagaa atactaatta cggcaattga gaaatgtcca
     2341 tcgccggaac tgttgctggc atacgcaaat cttgccggag atctgaaaac aaaggttgaa
     2401 tgcttcaaca aggcatttat gatgtcagga cttggccctg gccttaaaga gagttattca
     2461 cttttgcctt atttctttag gatcagatcg ctggaagtca ttcctgaagg tattcatgcc
     2521 gatgttgcca ggccgcttgt ttccataatc attcctactt ataattgcag tgacacaatg
     2581 ggttacgtca tggattcggt gttgagccag acctggaaaa acatcgaagt gatcgtggct
     2641 gatgactgca gcactgatca aactgccggt attgtcaggg ggtatttgca aaaagataag
     2701 cgggttaagt ttattcggac agaagctaac agcgggctgt ttgtagccat gaacaaggct
     2761 ttgcaaatag caaaaggaga atttgtgact tgccaggatc aatgtgaatg ggcgcatccg
     2821 gaaaagattt ccattcaggc gggccacctt gttgggaatc ccgattatac tgcaaatatt
     2881 tcccggcgga taaaggtgaa cagtaatctt gagccgctca gggccgaaca ttcagggctt
     2941 ttcattcaga ataatatccg atccttgatg ttcagaaggc aaagagtaat gacgagcctt
     3001 ggattctggg attctgtcag ggccggcgcc gacgaggaga tgcttttccg gctcaggcga
     3061 aaattcagtg ataaaaggat atgttacctt gaaaccgggc cattggcctt cctgttagaa
     3121 gaaaccagct catttgcaaa gaaaaggaac ccggacgttt cgcctggcgc tttgagagaa
     3181 tacatggaat catttaaatc atttttggcg aaaaatgatt ctccgtcgta tgaattcccg
     3241 cttaaagcga ggagataccc ggttccggag cccttctggc cggtggctga gaaaaagaca
     3301 aacggcaggc ggcattttga tgtcttacta gcgtcagagt tcaggttgcc cggcggtacg
     3361 accacgtcga acgtagagca gatcaaggct caaagggctg caggattaaa gacgggcctg
     3421 gttcacctta aacggtacga catcaataca tcggagccca tacgtgacaa gatcagggct
     3481 gaaattgacg gagaccttgt tcagatggtg gttcccgggg agaaagtatc gtgtgacctc
     3541 ctggtggtgc gacatccacc ggttttaaaa gatttccagt tatttgtgcc tgatatccag
     3601 gccgggcacc tgctggttgt catcaatact tcgccattac gggaatatag catacaggga
     3661 gaaagagttt atgatttccg
//
LOCUS       DAOQXS010000317         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26110_length_3675_cov_3.483702, whole genome shotgun sequence.
ACCESSION   DAOQXS010000317 DAOQXS010000000
VERSION     DAOQXS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26110_length_3675_cov_3.483702"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PKM34_08930"
     CDS             <1..1092
                     /locus_tag="PKM34_08930"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNQ83753.1"
                     /translation="HQGRAAENVLFSVLIKEGDYIPGNSHFDTTKGHIEFRKAHAVDL
                     TIDIASDTEAYHPFKGNVDIDKLDSYLAKNANKVPFVIVTVTNNSSGGQPVSMECLRE
                     VRRVADKYGKPVLYDSARFAENAYFIKMREKGYENKTIKEIVKEMFSYADFMTMSSKK
                     DAIVNMGGFIGFRNEELFRSASVFNILFEGYITYGGMSGRDMNALAQGLYEGTEFNYL
                     ETRVEQVAYLGKKLVEYGIPVQQPFGGHAIFVDARRFLPNVPKEQFQAQTLAVELYLE
                     GGVRGVEIGTLLADRDPVTRENRYPKLELMRLTIPRRVYTNNHMDYIAACLKNIYDRR
                     EEITSGLRIVREAPILRHFTIELDKFGDQ"
     gene            complement(1086..1661)
                     /locus_tag="PKM34_08935"
     CDS             complement(1086..1661)
                     /locus_tag="PKM34_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNQ83754.1"
                     /translation="MNSIKGTQTEKNLLKAFAGESQARNRYEFYAKEAKKEGLEQIAA
                     IFEETAENERSHAKQFFRFLEGGMVEITAAYPAGIIGNTLENLKAAADGENEEWTHLY
                     PEFARTAEQEGFKEVAAAFKIISKIEKAHEERYRKLYDNLESGKVFEREGKVVWKCRV
                     CGYLHEAKKPPLNCPACHHPQAYFELHTENY"
     gene            1799..2251
                     /locus_tag="PKM34_08940"
     CDS             1799..2251
                     /locus_tag="PKM34_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ83755.1"
                     /translation="MNKEERYQRIYNQLDELLETGHGPLSRMATIVAVLHHKMEYFFW
                     TGFYLIEKGELMVGPYQGSLACIRLKKDTGVCWAGINRAETVIVPDVHAFPGHIACDS
                     RSKSEIVVPLKDRSGIIMGVLDVDSKELASFDETDALWLQKITNLVYL"
     gene            2274..2831
                     /locus_tag="PKM34_08945"
     CDS             2274..2831
                     /locus_tag="PKM34_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HNQ83756.1"
                     /translation="MPYTILLFPLIISFIPLSVAVSTSIYRCVEWKEAFRTAIVFALF
                     QAAMMTIGWAIGYGVKGMLYSMAVPIGAVIVFFIGIRLMFDSRRLTRENRIMAVENRR
                     ILIGFALVSSINTALLGMGLGLLYPDILFLAAPVFGFTFLGVVAGVQAGKRGMVNLGR
                     NLELLGSLGLIIASIIIALQHLRIL"
     gene            2886..3332
                     /locus_tag="PKM34_08950"
     CDS             2886..3332
                     /locus_tag="PKM34_08950"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNQ83757.1"
                     /translation="MTIIRISKEFNFDMAHALLGYDGLCRNIHGHSYTLVVTIIGTPV
                     ADPASPKTGMLIDFKDLKNIIKTNIVDPYDHALVLNADTPKDLLDTLVRNYEKIVVTP
                     YQPTTENMIADLADRISRLLPGHVKLYSLKLRETPSSFAEWYAEDQ"
     gene            complement(3341..>3675)
                     /locus_tag="PKM34_08955"
     CDS             complement(3341..>3675)
                     /locus_tag="PKM34_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83758.1"
                     /translation="VGIDENADQDGFVIYPNPAGTWFSVGQHVLSPAKPGEGSHLDNS
                     APGLTSSKDHRIFIQNMLGERIREIEIQGNLDGIRIDVSECPNGLYFVSIYSDSVLKT
                     SNKLIILR"
BASE COUNT          920 a          899 c          903 g          953 t
ORIGIN      
        1 catcagggaa gggcagccga gaatgtgctc ttctcagtcc ttattaaaga gggcgattac
       61 atccccggca attcgcattt cgatactacc aaagggcata ttgagttccg caaggctcac
      121 gcagttgatt taaccatcga tattgcaagc gatacagagg cttaccaccc gttcaaaggg
      181 aatgttgata ttgacaaact ggatagctat ctggctaaaa atgccaacaa ggttcccttt
      241 gtcattgtaa cggttaccaa caactcatcg ggagggcaac ccgtttccat ggagtgtctg
      301 cgcgaagtga gaagggtggc agacaagtac gggaagcccg ttctctacga ctcggcccgt
      361 tttgccgaaa acgcctactt catcaaaatg cgtgaaaagg ggtatgagaa caagaccata
      421 aaagagattg tgaaagagat gttctcctac gccgatttta tgaccatgag cagtaagaag
      481 gatgcaatcg tcaatatggg cggatttatt ggttttcgga atgaagagct gttccgtagc
      541 gccagcgtgt tcaatattct ttttgaagga tacatcacct acggcggaat gtcggggcgc
      601 gatatgaatg ccctggcaca ggggctttat gaagggaccg agttcaatta cctggaaaca
      661 cgggtggaac aggtggctta cctcgggaag aaacttgtgg aatatggtat tccggtgcaa
      721 caaccttttg gcggacatgc aattttcgtc gacgcccgcc gcttcttgcc caatgtcccg
      781 aaagaacaat tccaggctca gacgctggcc gtggagttgt atctggaagg aggagtgaga
      841 ggggtggaga ttggcaccct gctggctgac cgcgacccgg tgacacggga aaaccggtat
      901 ccgaaactgg aactgatgcg ccttaccatc ccccggaggg tatatacgaa caaccacatg
      961 gattatatcg ctgcgtgcct gaaaaatatt tacgaccgcc gcgaagagat cacctcaggc
     1021 ctccggatcg tgcgagaagc ccccatactc aggcatttca caatagaact ggataagttc
     1081 ggggatcagt agttttcggt atgtaattcg aagtaggctt gcggatgatg gcaggccggg
     1141 cagttcagcg gaggcttttt ggcttcatgc aggtatccgc atacccggca cttccagacc
     1201 actttccctt cacgctcaaa cactttccct gattccaggt tgtcgtaaag cttcctgtaa
     1261 cgctcctcgt gcgctttctc tatcttcgag atgattttga aggcagcggc cacttcctta
     1321 aacccttcct gctcggcagt ccgggcaaat tcggggtata gatgggtcca ctcctcattc
     1381 tcgccatcgg cggccgcttt caggttctcg agcgtatttc cgatgatgcc ggcagggtaa
     1441 gcagcggtaa tttccaccat tccgccctcg agaaaacgga aaaattgctt ggcatgggag
     1501 cgctcatttt cagcggtttc ttcaaaaatt gcagcaattt gctccaatcc ttcttttttt
     1561 gcctctttgg cataaaattc atagcggttc cgtgcctgcg attcaccggc aaatgccttc
     1621 agcaggtttt tttcggtctg cgtccctttt atactgttca tgggtattgg tttttgttag
     1681 cgaataaaga tactgaatta tatttatttt tgatccctct tagtgtcttc gtgccttcat
     1741 ggtagaaatt aggagaagca acggcattta agatgatttc attattttaa ttatttaaat
     1801 gaataaagaa gaaagatacc aacggattta caatcaactc gatgagctgt tagaaacggg
     1861 ccatgggcct ttatcccgca tggcaacgat agtggcggtt ctgcatcata aaatggaata
     1921 tttcttctgg accggatttt acctaataga aaagggcgaa ctgatggtcg ggccttacca
     1981 gggcagcctg gcctgtatcc ggctgaaaaa agacaccggc gtttgctggg ccgggatcaa
     2041 ccgggcagaa acggtcatcg tacccgatgt gcatgctttc ccgggtcata tcgcctgcga
     2101 ttcgaggtca aagtcggaaa tcgttgtgcc actgaaggat cgtagcggta tcatcatggg
     2161 cgttctggat gtcgacagca aagagctggc ttcatttgat gaaaccgatg ccctgtggct
     2221 ccaaaagatt acaaacctgg tatatctctg atcattaaca tatagcctca gccatgccgt
     2281 atactatctt gcttttcccg ctcatcatca gctttatacc gctatcggtt gcggtaagca
     2341 ccagcattta ccggtgtgtg gaatggaaag aggcgttcag aacagcaatc gtctttgccc
     2401 tgttccaggc ggcgatgatg actattggct gggccatcgg gtatggcgtc aaaggaatgc
     2461 tttacagcat ggccgtgcca atcggcgcgg taatcgtatt ctttatcggc atcaggctta
     2521 tgttcgattc ccggcggctt acccgggaga accggatcat ggcggtggag aaccggcgca
     2581 ttttgatcgg attcgccctg gtatccagta tcaatacagc actcctcggg atggggctgg
     2641 gacttctcta cccggatata ctgtttttgg cggcccctgt cttcggtttt actttccttg
     2701 gcgtcgtagc cggcgttcaa gccgggaaac ggggtatggt caacctgggc cggaacctgg
     2761 agctcctggg aagcctgggc ctcatcatcg ccagtattat catagctctg caacaccttc
     2821 gcatattata aatcagcatt ttcaaattaa ctaattttca aatttccaaa ttatcacatt
     2881 tccaaatgac aatcatccgg atcagtaaag aattcaactt cgatatggct catgcactcc
     2941 tgggttatga cggcctgtgc aggaatattc acggacattc ctatacgctc gtggtcacta
     3001 tcatcggtac ccctgtggcc gatccggcat ctcccaaaac cggaatgctg atcgatttca
     3061 aagacctgaa gaacatcatt aaaacaaata ttgtggaccc ctacgaccac gcccttgtac
     3121 tgaatgccga taccccaaag gacttgctcg acacattggt caggaactat gaaaagatcg
     3181 tggtaacgcc ttatcagccg acaacagaga atatgatcgc cgacctcgcc gatcggatca
     3241 gccggttact gcccggccat gtgaaactat acagcctgaa actgcgggag accccaagct
     3301 cttttgcgga atggtacgcg gaggatcagt agtcagataa ttacctgaga ataatcagct
     3361 tatttgaagt ttttaatacc gaatcggaat agatagatac gaagtataag ccattcggac
     3421 attcggaaac atctatcctg atcccgtcaa gatttccttg tatttcaatc tcccggatcc
     3481 tttcacccag catgttttga ataaaaatcc tgtgatcctt tgaagaagtc aacccgggag
     3541 ccgaattgtc caagtgtgat ccttcgcccg gcttcgccgg actcaggaca tgctgtccaa
     3601 ctgaaaacca cgttccggcc ggattcgggt agatgacgaa accgtcctgg tctgcattct
     3661 catcaattcc tactg
//
LOCUS       DAOQXS010000318         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26126_length_3673_cov_3.926202, whole genome shotgun sequence.
ACCESSION   DAOQXS010000318 DAOQXS010000000
VERSION     DAOQXS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26126_length_3673_cov_3.926202"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKM34_08960"
     CDS             <1..667
                     /locus_tag="PKM34_08960"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83759.1"
                     /translation="AGRHGEPDILQEFSLREASYFSRLASGSACRSVYGGYTVWGESP
                     YVDGSHDEYAVPLPFEVHPVFRDIKDNILIVSSVEKKVSSRAGHSLMEGHPYAEARFR
                     LACQNIGILVSALSSGDIETFIRITESEALNLHALMMSSSSSYLLIEPNTVNIVNEIR
                     RFREETTIPVCFTLDAGPNVHLLYPAEYEENVKVWIGQVLVKYCENGRWIDDGTGTGP
                     IRL"
     gene            694..1644
                     /locus_tag="PKM34_08965"
     CDS             694..1644
                     /locus_tag="PKM34_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83760.1"
                     /translation="MKPEVFYAKIMLFGEYSVICDSMGLTIPYAHFTGSLRFINQEEY
                     TNYEMATESNRQLLNYLDHLKKLSREKEFKARMNLPKLESDIKDGLYFESNIPQGYGI
                     GSSGALVAAIYDQYSEGTQKHRKIYTSHEIPNLKETFSRMESFFHGISSGLDPLLCYI
                     RHPLLIRNKMQIETVGIPREKFGSDHAIFLIDTGELGKTGPLVNLFLERCKNDGFLNQ
                     VQNVFIPANNGCIETLLKGDINPFFGHLTELSAFQFLYLPEMIPDKMKSLWEAGLHRD
                     DFKLKLCGSGGGGFLLGISRDFRTTRKYLKQKGFEPITVF"
     gene            1654..2718
                     /gene="rlmN"
                     /locus_tag="PKM34_08970"
     CDS             1654..2718
                     /gene="rlmN"
                     /locus_tag="PKM34_08970"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNQ83761.1"
                     /translation="MNPRRNNIRTLSLTELETFFHSIGAEKFRARQVYEWLWKKAARS
                     FDQMTNISVETRAKLSESFTLDSITLNTTQKSEDGTIKMSFRLFDGHIIEGVLIPSGT
                     RMTACISSQVGCNLACKFCATGMIRYIRNLNFDEIFDQVAIIAETARHEYNLPLSHIV
                     YMGMGEPMLNYQNVLKSAERISAPDGLAMSPQRITVSTVGVAPMIRQLGNDKVKFHLA
                     LSLHSAINEKRNQIVPYNLRNSLGDLIEAVKYFHQQTRTRITLEYVMLKGFNDTMEDA
                     KALAEFCKNFPVKINLIQYNPVENVPYEDSGLERTEAFKAFLEKKNLVVNIRRSRGKD
                     IDAACGQLANKILKQNQENK"
     gene            2721..>3673
                     /locus_tag="PKM34_08975"
     CDS             2721..>3673
                     /locus_tag="PKM34_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ83762.1"
                     /translation="MDNSFSIRGLIVDVIGGRIFPGSVLVQDGRIQEIREHPVDETVY
                     ILPGLVDAHIHIESSMLIPSEFARLAVIHGTVATVSDPHEIANVLGIEGIKFMIENGK
                     KVPFKFCLGASSCVPATPFETAGASLGPKEVEELLRMDEIGYLSEMMNFPGVLKSDPE
                     VMQKLEIARKYGKPVDGHAPGLRGEDARNYISAGISTDHECFELDEALEKASMGMHIL
                     IREGSAARNFDDLSPLLESYPDQVMFCSDDRHPDDLLKRHINDIARRAINKGYDPVAV
                     IRACTLNPKKHYNLDTGLLQPGDPADLIVIDDLSSFHVLKT"
BASE COUNT          957 a          909 c          945 g          862 t
ORIGIN      
        1 cgccggccgg cacggagagc ccgacatcct tcaggaattt tcgctccggg aagcatccta
       61 tttctcccgc ctggccagcg gcagtgcatg ccgctcagtt tacggcggtt acaccgtctg
      121 gggcgaaagc ccctatgtcg acggctcaca cgatgaatat gccgttccgc tgccgtttga
      181 agttcatccg gttttcaggg acatcaagga taatatactg atcgtcagta gtgtggagaa
      241 gaaggtttcg agccgcgccg gccactcgct gatggaggga catccctatg cagaggcacg
      301 gttcaggctg gcctgtcaga atatcggaat tcttgtatcg gcattatcgt ccggcgatat
      361 cgaaacattt atccggatca ccgaatcgga agccctcaac cttcatgcgt tgatgatgtc
      421 gtcatcatct tcctatttat tgattgaacc gaatacggtt aatattgtta atgaaataag
      481 gagattccgt gaagagacga ccatccctgt ttgctttact cttgatgccg gaccgaatgt
      541 gcatctgctg tatcctgcag aatatgaaga gaatgtaaaa gtatggatcg gtcaggtact
      601 ggttaaatat tgcgaaaacg gcagatggat cgatgatggt accggtacag ggcccatcag
      661 gttataagga aattaaatta aaattaaaga taattgaagc cggaagtttt ttatgcgaag
      721 atcatgctgt tcggtgagta cagcgtcatt tgcgactcga tggggttgac cattccatat
      781 gcccatttca ccggttcgct caggtttatc aaccaggaag aatacaccaa ttatgagatg
      841 gcgaccgaat cgaaccggca actgcttaac tacctggatc atcttaaaaa attaagcagg
      901 gagaaggagt tcaaagcccg gatgaacctg ccgaaactgg agtcggacat caaagatggc
      961 ctttattttg aatccaatat tccgcaaggg tatggtattg gcagctccgg ggcactcgta
     1021 gcagcaatct atgaccagta ttcggaaggt actcaaaaac accggaaaat atataccagc
     1081 catgaaatac cgaacctgaa ggagactttt tccaggatgg aatctttctt tcacggcatc
     1141 agctcaggcc ttgatccgtt actttgctac atcaggcacc cgctgctcat ccggaataaa
     1201 atgcagatcg aaacggtggg cataccgcga gagaaattcg gttccgacca cgccattttc
     1261 ctgattgata cgggtgagct tggcaaaacg gggccactgg tcaatctctt cctggagcgt
     1321 tgtaaaaatg acggattttt gaaccaggtg cagaatgttt ttatcccggc taacaatggc
     1381 tgtattgaaa ctcttctcaa aggcgacata aacccgttct tcgggcatct gacagaactt
     1441 tccgctttcc agttcctgta tctgccggag atgatccccg ataaaatgaa atccctctgg
     1501 gaagccggat tgcaccgcga cgatttcaaa ctgaaactgt gcggttcggg tggcggggga
     1561 ttcctgctgg ggatatcccg ggatttcaga accacgagaa agtacctgaa gcaaaaaggt
     1621 tttgaaccga ttaccgtatt ttagaaaaag aaaatgaatc ccaggcggaa caatatcagg
     1681 acgctgtcgc tgacagaact ggaaaccttc tttcattcga tcggcgctga aaaattccgg
     1741 gcccggcagg tgtacgaatg gctctggaaa aaagcggccc ggtcgttcga ccagatgacc
     1801 aatatttccg tcgaaacccg ggcaaagctg agtgagagct tcaccctgga ttctatcacc
     1861 ctcaacacaa cccagaagag cgaagacgga acgatcaaaa tgtcgttccg gctgttcgac
     1921 gggcatatca tcgaaggggt gctgatcccc tccggaacga ggatgacagc atgcatctcc
     1981 tcgcaggtag gctgcaacct ggcttgcaag ttttgcgcga ccggtatgat ccgctatatc
     2041 cgcaacctga atttcgatga aattttcgac caggtggcca ttattgccga aaccgcccgc
     2101 cacgaataca atctcccgct ttcgcatatc gtttatatgg gaatgggcga acccatgctg
     2161 aattaccaga acgtcctgaa atcggcagag cggatttcgg cacctgacgg cctggcgatg
     2221 tcgcctcaac ggatcaccgt atcaacggtc ggcgtggcgc cgatgatccg tcagctcggc
     2281 aacgacaaag tgaagttcca cctggccctg tcgctccact ctgccatcaa tgagaaacgc
     2341 aaccagattg tcccttataa tttgcgcaac agcctgggcg acctgatcga ggcggtgaaa
     2401 tatttccacc agcagacccg tacccggatc acactcgaat atgtgatgct gaaaggattt
     2461 aacgatacca tggaggatgc gaaagcgctg gctgagttct gtaaaaattt cccggtcaag
     2521 atcaacctga tccagtacaa cccggtggag aatgtccctt acgaagattc cggattggag
     2581 cgaacagaag cgttcaaggc cttcctggag aagaaaaacc tggtcgtcaa tatccggcgg
     2641 agccgcggaa aagatatcga tgctgcctgc gggcaactgg ccaataaaat tctgaaacaa
     2701 aaccaggaaa ataaataacc atggataatt cattttcaat ccgcggcctt attgtcgatg
     2761 tgattggggg acggatcttc cccggcagcg ttctggttca ggacggtcgg attcaggaaa
     2821 tacgcgaaca tcctgtcgac gaaacggtat atattcttcc gggactggtc gatgcccata
     2881 tccacatcga aagttccatg ctgatcccgt cggaattcgc ccggctggct gtgatccatg
     2941 gaacggtggc aacagtctcc gacccgcatg agattgctaa tgtgcttggc attgagggga
     3001 ttaaattcat gattgagaat gggaaaaaag ttccctttaa attctgcctc ggggcctctt
     3061 cctgtgttcc tgccactccg ttcgagacag ccggtgcctc gctggggcca aaggaggtgg
     3121 aggaactgct ccggatggat gaaatcggtt atctctcgga aatgatgaac ttcccgggtg
     3181 tccttaaaag cgatcccgag gtaatgcaaa agctggagat agcccggaaa tacggcaagc
     3241 cggtcgatgg ccatgcgccg gggctcaggg gggaagacgc ccggaattac atctctgccg
     3301 gtatttctac cgaccatgag tgttttgagc ttgatgaagc gctcgaaaaa gcgtcgatgg
     3361 ggatgcatat ccttatccgc gaaggaagcg ccgcgcggaa tttcgacgat ttgtcgccgt
     3421 tgctggagag ttacccggac caggtgatgt tctgttccga tgaccgccat cccgatgatt
     3481 tgctgaagcg ccacatcaat gatattgcac gccgcgcgat aaacaaaggg tacgatccgg
     3541 ttgcagttat ccgtgcctgc accctgaatc ctaaaaagca ttacaacctg gataccggac
     3601 tgcttcaacc cggcgatccg gctgatttaa ttgtgatcga tgacctttca tctttccatg
     3661 tgctgaaaac cta
//
LOCUS       DAOQXS010000319         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26165_length_3670_cov_3.749931, whole genome shotgun sequence.
ACCESSION   DAOQXS010000319 DAOQXS010000000
VERSION     DAOQXS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26165_length_3670_cov_3.749931"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2443
                     /locus_tag="PKM34_08980"
     CDS             110..2443
                     /locus_tag="PKM34_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ83763.1"
                     /translation="MKKFITLTLIAFFATSLFAGRIIHSYNSISPTAKDVGNLNMIMF
                     EGMYLTGKTGEPVLPYQAVKLLLPPGEEAVSVIWKFENELIIAGNFTLYPQQPVQPYS
                     VGAQGIFQQNMDVYSSGNVYPAEAMGRYSTHFLNGHGFLLATFTPVKYIPATGQVSFF
                     QKVTIQVETRKTERAERAFRNINNNDRINSVIKDFAQNPEMISSYPEKLYRDGEYQYL
                     IITPEQFAPSFQALTDLYRVRGYITEIATTESIDATMPGQDLQEKIRNYIIAAVQANG
                     VEHVLLGGDVEHIPHRGFYCHVQSSSVYEDWNIPADLYYSALDGNWDTDGDGIWGEIG
                     EDDLLPDVAVTRMPISTSTQLQRMLNKTIMYQDQPVAGEMRDIISAGENLWSDPLTYG
                     EDYLELLFGWHDDNGYVTDGIPEDYDVNRLYDSQMYWDKYTLMNAINQGSSFIHHSGH
                     ANQTYVMRFDMSDISNENFAQVNGTDHNYALIYTHGCLCGAFDDSDCIGERMVLIDNL
                     AVAGGFNSRYGWFNEGQTEGPSAHLHREFVDALYHEKEWRIGAAHMISKIETSVWVNA
                     PGQWEQGALRWCFYDCNIFGDALLGVWTEEPVSIETTYPEEIYPGETTVQVSIANGDG
                     PAVGFNCVIMSAGEMIGCATTDESGQAEISVPEGFIDHLDAQLIVSGYNCLPHTYDLN
                     IVVGIDEPYVIDYLNIIPNPFRDQTSLVYQLEEAGSIEVTFVNLAGQMIEKMQFDGKK
                     GINRFSINTTEWPEGMIISKITGENRTDYYPLLHIKN"
     gene            2552..>3670
                     /locus_tag="PKM34_08985"
     CDS             2552..>3670
                     /locus_tag="PKM34_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNQ83764.1"
                     /translation="MNRILLLSSFILLMMSGLDAQTYRYDDSFGPQGFSLVSHDRLGV
                     TVNFSIREFSMEEMDIKGDRMQHISLPGHFLPGDEGTPDLPGDGRFIAVPNGAKPVIS
                     VKTARREVIRNVDIAPAPNLPLDNDDRPVVYKKNAKIYSTDAYFPAEPVKLSGKMSIR
                     GVDAFVLGITPFQYNPVKRELVILRDMEVEIRFEGGSGDFGEERFRSRWWDPLMQDMF
                     LNSADLPVIEYSKRVTSPNRATGCEYLIICPDGEEFIAWADTIRRFRNQQGILTEVFT
                     ISQVGGNNAMIIEDFINNAYYSWEIPPAACLLLGDYGSLPQNAITSSLLNDHPDDYNP
                     YISDNPYADVTNDLLPDIAFARITARNAAELELMIGKAL"
BASE COUNT          938 a          889 c          941 g          902 t
ORIGIN      
        1 catttaacct ttgaatgtat tactgatcat agaaatattt aatattgtaa cggtattttg
       61 aatggaattt taaaaatatt tagacgctca tttcaaatct ccaaccccga tgaaaaaatt
      121 cattaccctt accctgattg ctttctttgc cacatcgctc ttcgccggcc gcatcattca
      181 cagctacaat agtatatcgc ctacggcgaa agatgtggga aatcttaata tgattatgtt
      241 cgaggggatg tacctgacgg ggaagaccgg agagccggtt ttgccctacc aggcagtgaa
      301 attgctgctc cctccgggcg aagaggcggt ctcggttatt tggaaatttg aaaatgagct
      361 aattattgct ggtaatttta ccctgtaccc gcaacaaccg gttcaaccgt attctgtcgg
      421 cgctcaagga atatttcaac agaatatgga tgtttattct tcgggtaatg tttaccccgc
      481 agaggctatg ggacggtact ccacccactt tctcaatggt cacgggtttc tcctggccac
      541 ttttacaccg gtgaaatata tccctgccac gggtcaggtt tctttctttc aaaaagttac
      601 catacaggtt gaaacccgga aaacagagag ggcggagaga gcatttcgca atatcaataa
      661 caatgacagg atcaatagtg ttattaaaga ttttgcacag aatccggaga tgatttcatc
      721 gtatcctgaa aagttatacc gcgacggaga ataccagtac ctgatcatta cgccggaaca
      781 gtttgccccg tcgttccagg ctcttaccga cctgtacagg gtcaggggat acatcactga
      841 aatcgctacg accgagtcga tcgacgcgac catgcccggc caggatctcc aggaaaagat
      901 caggaactat attatcgctg ctgttcaggc caacggagtg gagcatgtcc tcctcggcgg
      961 ggatgtggaa catatcccgc accgcggttt ctattgccat gtccagtcaa gctccgttta
     1021 cgaagactgg aatatcccgg ccgacctgta ttacagcgca ctcgacggga actgggacac
     1081 cgacggcgac ggaatttggg gcgagatcgg cgaagatgac ctgctgccgg atgtggcagt
     1141 gacccggatg cccatcagca ccagcactca actgcaacgg atgctgaaca agaccatcat
     1201 gtaccaggat cagcccgttg ccggtgaaat gagagatatt atttctgcag gcgagaacct
     1261 ctggtcggat ccgctcacct acggagaaga ttacctggaa ctgcttttcg gatggcacga
     1321 cgataacggt tatgttaccg acggcattcc tgaggattac gatgttaacc ggctctacga
     1381 ctcgcaaatg tactgggata aatacacgct gatgaacgcc atcaaccagg ggagcagttt
     1441 catccaccat tcgggccatg ccaaccagac ctatgtgatg cgctttgata tgtcggatat
     1501 cagcaacgaa aatttcgcgc aagtcaacgg taccgaccac aattacgctt tgatctatac
     1561 gcacggatgc ttatgcggcg ctttcgacga tagcgactgc atcggggaac ggatggtgct
     1621 gatcgataac ctggcagtgg ccggcggatt caattcgcgt tacggctggt tcaacgaagg
     1681 gcagacggaa ggcccgtcgg cccacctgca ccgggaattt gtagatgcgc tttaccacga
     1741 aaaagaatgg cggatcggag ccgctcatat gatatccaaa atagagacct cggtttgggt
     1801 gaatgcaccc ggccaatggg aacagggtgc cttgcgctgg tgcttctacg attgcaatat
     1861 cttcggcgat gctttactcg gcgtttggac cgaagagccg gtgtccatcg agaccactta
     1921 cccggaggag atctacccgg gcgagaccac ggtgcaggtg agcattgcga acggcgacgg
     1981 gcccgccgtg ggctttaact gtgtgattat gtctgccggc gaaatgattg gctgcgcgac
     2041 taccgacgaa tcgggccagg ccgagatcag tgtgcccgaa ggtttcatcg accacctgga
     2101 tgcccagctc attgtttcag gctataactg tttgcctcat acctacgatc ttaacattgt
     2161 tgttggaatt gatgagcctt atgtgattga ttatcttaat attatcccga atccgttcag
     2221 agatcagaca tcgctggttt accaactcga agaggccgga agtatcgaag taacttttgt
     2281 aaacctggcg ggtcagatga tcgaaaaaat gcaattcgac gggaagaaag ggataaacag
     2341 attcagtatc aatactacgg aatggccgga aggaatgatc atttcgaaga ttaccggcga
     2401 aaaccgcacc gattattatc cactcctgca catcaaaaat tagcggagaa ttattttgaa
     2461 ttttctcttt tttttgatat aattttgtaa ttattcaaat tcaggattct gcatatgaaa
     2521 gaatcctgaa ttcaacataa cgattacaat tatgaacagg atacttttac tttcatcttt
     2581 tatattattg atgatgagcg gtctggatgc ccagacctac cgttatgacg acagtttcgg
     2641 cccgcagggt ttcagcctgg tttcgcacga ccggctgggt gttacggtga acttctccat
     2701 ccgggaattt tccatggaag agatggatat caaaggcgac aggatgcaac acatcagtct
     2761 tccggggcat tttcttcccg gtgatgaagg gactcccgat ctgccgggcg acgggcgctt
     2821 tattgccgtt ccaaacgggg caaaacccgt gatctcggtg aaaacggcca ggcgggaagt
     2881 gatccgcaat gtggatatag ccccggcacc gaacctgccg ctggataatg acgaccgacc
     2941 ggtcgtttat aaaaagaacg caaagattta cagcaccgac gcctatttcc cggcggagcc
     3001 ggttaaatta tcggggaaga tgagtatccg cggcgtggat gcatttgtac tggggatcac
     3061 accattccag tataacccgg taaagcgcga gctcgttatt ttgagggata tggaagtgga
     3121 gatcaggttt gaaggcggca gcggtgattt cggggaagaa cgctttcgca gccgttggtg
     3181 ggatccgctg atgcaggata tgttcctgaa ctccgccgat cttcccgtta ttgaatattc
     3241 gaagagggta acctcaccga acagggccac cggatgcgaa tacctgatca tctgccctga
     3301 cggtgaagaa tttatagcct gggccgatac gatccgcagg ttcaggaacc agcagggtat
     3361 cctgacggag gttttcacca tatcgcaggt gggtggcaac aacgccatga tcatcgagga
     3421 ctttatcaat aacgcctatt attcgtggga gattccgccc gccgcctgcc tgctcctggg
     3481 cgattacggt tcattgcctc agaatgccat cacttcgagc ctgctgaacg atcatcccga
     3541 cgattacaat ccttatattt ccgataatcc gtatgccgat gtgaccaacg acctgctgcc
     3601 cgatatcgct tttgccagga tcacggcccg gaatgcggct gaactggagc tgatgatcgg
     3661 gaaagcgttg
//
LOCUS       DAOQXS010000320         3658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26272_length_3658_cov_4.264502, whole genome shotgun sequence.
ACCESSION   DAOQXS010000320 DAOQXS010000000
VERSION     DAOQXS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26272_length_3658_cov_4.264502"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..800)
                     /locus_tag="PKM34_08990"
     CDS             complement(18..800)
                     /locus_tag="PKM34_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83765.1"
                     /translation="MSSKNKGVREILSFQLGLKYLIIFLLTIIGEQMTCAQNLNPGIC
                     LNPEINAEFKYGAEELNSFIANNLIVPYKARYYGINGDVELLYIVDTLGNIGEITVKI
                     ENIDLGPALYAREGFGNESFSGFFSNEAIRVIRLLSGLYKPALHQGMKVSSWLSITLS
                     FKTKQYDENERQVRVDKASKVKRIEWNFGIYTQEEPDYALIRYDLGVLKMEENKLEIA
                     CKYFEEALRMKSDYVDALFNLGIAYARLQQTEKACQMKSAST"
     gene            complement(897..1853)
                     /locus_tag="PKM34_08995"
     CDS             complement(897..1853)
                     /locus_tag="PKM34_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83766.1"
                     /translation="MKKTSMLFFAVLLSAAAFAQPVINKLYDFQPGDSYTYKKLAPGG
                     SIDYNSIETAGANLVWDLSFLQLEENPYTDTIIAYENSDWPNSFPGCNFVWKEYSGTE
                     QYYRKNGDSLLYMGNAVWAPSKFEPNPPTIVYPGNYTASGYMYSGFQSYLNSSALWTF
                     EARYNAYGTLVLPGNINVPNVALYSSYGGPSNSGYSDFMWVRENESLPIMRIQFYHSG
                     SSITVQYCYVLHAALTGIETIDETLVRAHVYPNPAKDFIYIESEQELSETKLTDLSGR
                     ILYQTKGSGNKVVIPAGDLTEGIYLVKYTDRNGRSAVTRVVR"
     gene            2006..2758
                     /locus_tag="PKM34_09000"
     CDS             2006..2758
                     /locus_tag="PKM34_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83767.1"
                     /translation="MKRLAILVSYVMVLCTGAMAQSNHTHGHMLSVNHLQIKESLNYG
                     LVFKGPGLGYKYSSLWENKRRIMEYTGALSLNVPLTRGIISGSVNLVPARFDYRIKFD
                     PEGKVSFGPYVILDYNMMIYPDLQSGYTFWLTNYSLGGVLSGWFTSKENRFDLCLHFT
                     VMGFTSRTEPYDDPYFFSGSFGDLIGDLRSDLTFNPGRYTVNEIELKWTPNPSSRLAY
                     GYSFRYHGYFQEPELNMINHSVIIHFLPKKDK"
     gene            2760..>3658
                     /locus_tag="PKM34_09005"
     CDS             2760..>3658
                     /locus_tag="PKM34_09005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNQ83768.1"
                     /translation="MKKICTLLCFVIIILAVPGCLLEDELKQSYTGFVPLEIGDGWAI
                     SSPVTENIDSAALENIYKEVYADDNLWMTKSMLVFRNGKLVSESYLKDDADRTRIDAV
                     WSCTKQVNAIITGIAIREGLITGVGATIEDYLPEYASKYPDKAGITLENLLMMAGGIS
                     FDNGYQSDVFRTHSTKNSLDFVLSMDLNFEPGDGFWYNDGEPQVVSGMVQAATGKPMD
                     EYAKEVFFDRIGMTNYEWVRYSDGVTLGGFGIMTSPRELAKIAQCVLDSGKAGGQQVI
                     PLDWIMEMTSPKVTVDELHSFGY"
BASE COUNT         1003 a          780 c          819 g         1056 t
ORIGIN      
        1 cttcggatga aatccgatta tgttgatgca cttttcattt ggcaagcctt ctcggtttgc
       61 tgtaatcttg catacgcaat cccaaggttg aaaagtgcat ctacataatc ggatttcatc
      121 cgaagggctt cctcaaaata tttacaagct atctctaact tgttttcctc catttttaaa
      181 acacccaaat cgtatcttat caacgcataa tcaggctctt cctgtgtata aattccaaag
      241 ttccattcta ttctttttac tttgctggct ttatcgaccc tcacctgtct ctcgttctca
      301 tcatattgct tggtcttaaa ggagagggtt attgatagcc aggaagatac tttcatacct
      361 tgatgcaaag cgggtttgta gagtccggac agcaacctga ttacacggat tgcttcatta
      421 ctgaaaaacc cggaaaatga ttcgtttcca aaaccttccc tcgcataaag cgccggaccc
      481 agatcgatat tttcaatctt caccgttatt tctccaatat tacccaatgt atcaacaata
      541 taaagcagtt cgacatctcc attaattcca tagtacctcg ctttataagg gacaattaaa
      601 ttattagcga tgaaactgtt taattcttca gctccgtatt taaattctgc attgatttca
      661 ggattgaggc aaatacccgg gttcaaattc tgagcgcagg tcatttgttc tccgataatt
      721 gtcagtaaga atataattaa atatttcagg ccaagctgga aagataagat ttctctgacg
      781 cctttgttct ttgacgacat aaactgcatt tttcaaattt acacagatta tatcgcaaat
      841 atatgtagaa ctaaccaaaa tgtaaaaaag cggcgtacat ggagtacagg gattcgttaa
      901 cggacgaccc tggtcactgc cgaccttcca tttcgatccg tatatttcac caggtatatg
      961 ccttctgtta gatcacctgc ggggataaca accttgtttc ctgatccttt tgtttgatat
     1021 aaaatccttc ctgacaaatc tgtcagcttt gtttcactga gttcttgttc agattcgatg
     1081 taaatgaaat ctttagcagg attaggatac acatgagccc ggacgagggt ttcatctatt
     1141 gtctcaattc cggttaaagc ggcatgaagc acataacagt attgaacggt aatggaagat
     1201 cctgaatggt agaactggat tctcattatg ggtaaggatt cattctcccg aacccacata
     1261 aagtcggagt aacccgaatt ggaggggcca ccgtagcttg aataaagggc aacattcgga
     1321 acattaatat tgcccggcag gaccagggtg ccgtaagcat tgtaccgggc ttcgaaagtc
     1381 catagcgcgg aagaattcag ataggattgg aagcctgaat acatataacc tgaggcagtg
     1441 taatttcccg gataaacgat agtgggaggg tttggctcaa atttcgaagg cgcccaaacg
     1501 gcattgccca tgtacaggag cgaatcgccg tttttcctgt aatattgctc cgtcccgctg
     1561 tattctttcc atacaaaatt acatcccgga aaagaattcg gccagtccga attttcataa
     1621 gcaataatcg tatctgtgta aggattctct tccagctgaa gaaatgacag gtcccagacg
     1681 agattggccc cggcagtttc aatactgttg taatcaatgc ttccgcccgg agccagtttc
     1741 ttgtatgtat aactgtcgcc gggctgaaaa tcgtaaagtt tgttaatgac aggctgtgca
     1801 aatgcggcag ccgacaaaag aacggcaaag aaaagcatag atgtcttttt cataatttga
     1861 tctgattaat taggttattt ttcgcaaaaa tagtcaattt agaaacgatc taaataaaaa
     1921 ttaatttaat actgttatgt ttcttgaaga agcatttatt tataacttgt gccattatta
     1981 tccaaaacaa aatcaacatc ttaccatgaa aagattagct attctggttt cttatgtgat
     2041 ggtgctttgt accggggcca tggcacaatc aaaccatact cacgggcata tgttatctgt
     2101 aaaccatctg caaatcaagg aatcattgaa ttatggcctc gtattcaaag gcccgggatt
     2161 gggttacaag tattcatccc tgtgggaaaa taagcgcagg ataatggaat atacgggcgc
     2221 gttgtcactg aatgtaccgc tcactcgtgg cattatttcc ggatcggtca atctggtgcc
     2281 tgcccggttc gattatcgca ttaaattcga tccggaaggc aaggtctcct ttgggcctta
     2341 tgttattctt gattataaca tgatgatcta ccccgatctg cagagcggtt acacattctg
     2401 gctgaccaac tacagcctgg gaggcgtctt gtcgggatgg tttacgtcga aagaaaaccg
     2461 tttcgatctt tgcctgcatt ttaccgttat gggatttacc tcaaggacag aaccctatga
     2521 tgatccctat ttttttagcg gttctttcgg tgatctgatc ggtgacctgc gctcggatct
     2581 gacgttcaat cccggacgat acaccgtcaa tgagatcgaa ctgaagtgga ctccgaatcc
     2641 ttcgtcaagg ctggcctacg ggtattcatt ccggtatcat gggtattttc aggaaccgga
     2701 gttgaacatg atcaaccatt ctgtaataat tcattttctt ccgaaaaaag ataaataaga
     2761 tgaaaaagat ctgtacttta ttatgttttg ttatcattat tctggcggtt ccgggatgtt
     2821 tgctggaaga tgagctgaag caatcgtaca ccggatttgt cccgctggag atcggcgatg
     2881 gatgggctat ttcatctccg gtaacggaga atatcgattc agcggcactg gagaatattt
     2941 ataaagaggt ctatgccgat gataatttgt ggatgacgaa aagtatgctg gttttccgta
     3001 acggcaaact ggtttcggag agctacctga aggatgatgc cgaccggacc cggatcgacg
     3061 cggtatggtc atgcactaag caggtgaatg ccatcattac aggtattgcc atccgggaag
     3121 gccttatcac cggcgtcggc gcaaccattg aggattacct gccggaatac gcctcaaaat
     3181 atccggacaa ggccggtatc acgctggaaa acctgctgat gatggccggc ggcattagtt
     3241 ttgacaatgg ttaccagtcg gatgtgttca ggacacattc cacgaaaaac agcctcgatt
     3301 ttgttctttc catggatttg aattttgaac ccggcgatgg cttctggtac aatgatggcg
     3361 aaccgcaggt cgtctcaggc atggttcagg ccgcaactgg caaaccgatg gatgaatacg
     3421 caaaagaggt gttttttgac agaataggga tgaccaatta cgaatgggtc aggtattcag
     3481 atggtgtcac cctgggtggt ttcgggatca tgacctcgcc gagggagctg gcaaaaatag
     3541 cccagtgtgt gctcgacagc gggaaggccg gcggacagca ggtgatcccg cttgactgga
     3601 ttatggaaat gacctcgccg aaagtgaccg tggatgagct ccattcgttc ggttatta
//
LOCUS       DAOQXS010000321         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26329_length_3652_cov_4.169864, whole genome shotgun sequence.
ACCESSION   DAOQXS010000321 DAOQXS010000000
VERSION     DAOQXS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26329_length_3652_cov_4.169864"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKM34_09010"
     CDS             complement(<1..470)
                     /locus_tag="PKM34_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ83769.1"
                     /translation="MHSFYILLTLVLMAVIFGIIEYFLHQKRISSIPIRIHVNGTRGK
                     SSVTRLIGAGLREGGITNITKVTGTFPRLILEDGTETYIHRKASANIIEQLSIVRFAA
                     SRKVKALVMECMALQPQYQTITEHRMIHSNVGVMTNIRMDHTDVMGHTLGEIAET"
     gene            complement(483..>3652)
                     /locus_tag="PKM34_09015"
     CDS             complement(483..>3652)
                     /locus_tag="PKM34_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ83770.1"
                     /translation="AINPRTALKRPIAPSFGKVYRSTIFNYQEVLNEQYGGREDGRDL
                     ILCIMPDEFYNSFQVYAEWKRQSGTDVHVTRFSDIGANANNPDIIKNHIADAYHNWEY
                     PPTYVLIVGDDGVFPKKIVTYPDYSFPNEDYFVEVDGNDYFPEMMIGRFTNQGDYRMQ
                     VMINKFLLYEKTPNTVTTDWFKKGTVCSNNAYESQKETKRFAAGMMLNYGHFTSVDTL
                     MSDGSGWGWDCTVSLDDVIDAINEGRSYLNYRGEGWTSGWQASCYNFETSDVTSLTNG
                     EHFTFVTSIGCGVAMFDTWGANNCFGEEWIEIGSLTAPKGGVAFVGPTSNTHTTYNNK
                     IDKGIYTGMFAEDMETPGQALLRGKLYMYNVYGDEYYVEYHYKIYCILGDPSIHIWRD
                     VPLAVTVDYPQTILVGQDQVEITVNFTASGQPAANAQVCLTGDEIFATGYCDSEGKVY
                     MDIVPETPDTLKLTVRGKNVYPFQADIIVIQPEELVEPDGGPALDDLDGNLDGLIDPN
                     ENGTATFTLKNWGSQTANNVMATLTSGMPDYMQVLTTVPVNYGNLASGAKITGNPFML
                     RVAENCPVGQLIPVQLHVTSTENSWDYSYDIVINGCNLLDEGFYVNDQGAAEPNYRMD
                     PGETVKLFIAVHNAGNDLAKDVVGILTSDDPFIDIEDGAGAFGTIAINETAINFYNYF
                     VVKIDPSCPADYFAQFNLQLTTSNGNYPYQKNLTLNLPVSHPVPSDYTGPDAYGYYAY
                     SSDDIFDQAPGYTWIEIDQAGTPINISGIGDYTETVSLPFTFKYYGVEYAQLRISTDG
                     WIAFGNGNQTAPVNTTLPNIDNINCMVAGFWDDLYDAETVEGNIYYYNDFSNHRVIIE
                     WDSIARNDFGSEPNVEIFQAVLYDPDFYPTLTGDGEIVFNYKQVRAPESSTIGIENQG
                     QNIGLLYLFNDAYNPGSNILKNEFSIKFTTEPPEASIITGIDDRKEGSMETSDHLSCF
                     PNPFVESTRISYTIDNPAAVSVKILDVEGRTVRILKSARQNAGTYEILWDGCNDAGLP
                     VSAGVYFCQIKSENFRETVKLFLLR"
BASE COUNT          887 a          922 c          843 g         1000 t
ORIGIN      
        1 gtctcagcaa tttcaccgag agtatggccc atcacatcgg tatggtccat ccggatattg
       61 gtcatgactc cgacattgga gtgaatcatc cggtgttccg taatcgtctg atattgtggc
      121 tgcaatgcca tacattccat caccagcgcc ttgacctttc gggatgcggc aaaccggacg
      181 atcgataatt gttcgatgat attggccgaa gctttccggt gaatgtaggt ttctgtgcca
      241 tcttccagga taagccgggg aaacgttccg gtcacttttg tgatattggt tattccgcct
      301 tcccttagcc ctgccccgat cagccgcgtc acactcgatt tccctctcgt cccgttcaca
      361 tggatcctga tcgggatgga tgaaatcctt ttctggtgca gaaaatattc gatgatcccg
      421 aatatgactg ccatcagtac cagggtaaga agaatataaa aactatgcat taataacgtg
      481 aactatctca ataagaacaa tttaacggtc tcccggaaat tttctgattt tatttggcag
      541 aaataaaccc cggcacttac cggtaaacct gcatcattgc atccatccca cagaatctcg
      601 taggttccgg cgttttgccg ggcgctcttt aatatcctca ccgttctgcc ctctacatcg
      661 agaattttca ccgatacggc cgccggattg tcaattgtat agcttatccg cgtcgactcc
      721 acgaacggat tcgggaaaca gctgagatga tccgacgttt ccatcgatcc ctctttcctg
      781 tcatcaatcc cggtgataat tgaggcttcc ggcggttcgg tcgtgaactt gatggaaaac
      841 tcgtttttca ggatatttga gccgggatta taggcgtcgt tgaacaggta tagcagaccg
      901 atattctgcc cctggttttc gatgccgatg gtgctgcttt caggtgcccg aacctgtttg
      961 taattaaata cgatctcacc gtcacctgtc aatgtgggat aaaaatcggg atcataaagc
     1021 accgcctgga aaatctccac attgggttcg gaaccgaaat cgttccgggc tatgctgtcc
     1081 cattcgatga taacccggtg attggagaaa tcgttgtaat aataaatatt cccctccacg
     1141 gtttcagcgt cataaagatc gtcccagaaa ccggccacca tgcagttgat attatcgatg
     1201 ttcggaaggg tggtgttgac cggtgccgtc tggttaccgt ttccgaaagc tatccagccg
     1261 tcggtactga tccggagctg ggcatattca acgccataat acttgaatgt aaaggggaga
     1321 ctgactgttt cggtataatc tcctattcct gaaatattga tcggtgtacc ggcctgatcg
     1381 atttcgatcc aggtataacc gggtgcctgg tcgaaaatat catcactgga gtatgcataa
     1441 tagccatagg catccggacc cgtgtagtcg gaaggcaccg ggtgcgatac aggcaggttc
     1501 agggtgagat tcttctgata tggataattc ccgttggaag tcgtcaattg aaggttgaac
     1561 tgggcgaaat aatcagccgg gcaggaaggg tcaattttta ccacaaaata attgtaaaaa
     1621 ttaatggctg tttcattgat tgcaatcgtt ccaaatgctc ccgctccatc ctcaatatca
     1681 ataaacggat catcggatgt caaaataccc accacatctt ttgccaggtc attgccggca
     1741 ttgtgaacgg ctataaaaag cttgaccgtt tcgcccgggt ccatccggta attcggttcg
     1801 gcggctccct ggtcattcac ataaaatccc tcatccaaaa gattgcaccc gttgataaca
     1861 atatcataac tgtaatccca ggaattttcg gtactggtaa catgcagctg aaccgggatc
     1921 aactgtccga cagggcaatt ttcagcaacc cgcagcatga atgggtttcc tgtaatcttt
     1981 gccccggagg caagatttcc gtagttgacc ggcacggtgg tcaatacctg catataatca
     2041 ggcatacccg atgtcagtgt tgccatgaca ttgtttgcag tctggcttcc ccagtttttc
     2101 agtgtaaagg tggctgtgcc gttttcgttt gggtcgataa ggccgtcgag gttgccatcc
     2161 aggtcgtcaa gcgccggtcc tccatccggc tcaacgagtt cttcgggctg gatcacgatg
     2221 atgtcggcct ggaaaggata aacatttttc cccctgacgg tcagttttaa agtatccgga
     2281 gtctccggga caatatccat gtagactttc ccctcactat cacaatatcc tgtcgcaaag
     2341 atctcgtcgc cggtaaggca cacctgtgca ttagccgccg gctgaccgga tgcggtgaaa
     2401 ttcaccgtga tctccacctg gtcctgtccc accaggatgg tttggggata atcaacggta
     2461 acggccaggg gaacatccct ccagatatgt atgctgggat cacccaggat acagtatatt
     2521 ttataatgat attcaacata gtattcatca ccataaacat tatacatata taactttccc
     2581 ctgagcaagg cctgaccggg agtttccata tcctcggcaa acatgcctgt ataaatccct
     2641 ttgtcgattt tgttattata ggttgtatgc gtattggaag taggcccgac aaaggctaca
     2701 ccgcccttgg gagccgtcag ggatccgatc tctatccact cttccccgaa gcagttattg
     2761 gctccccatg tatcgaacat cgctaccccg cagcctatgc tggttacaaa ggtaaaatgc
     2821 tctccgttgg tcaatgaggt tacatcagat gtttcgaaat tatagcagga ggcctgccat
     2881 ccgctggtcc agccttcccc gcggtaattc aggtagctgc ggccttcatt gatcgcatcg
     2941 atcacatcat caaggctcac cgtgcagtcc catccccatc ccgaaccgtc gctcatcaag
     3001 gtatccaccg aagtgaaatg tccatagttc agcatcatgc cggccgcaaa ccgtttggtc
     3061 tctttttgcg actcatatgc attattggaa cataccgttc cttttttgaa ccagtcggtg
     3121 gtcacagtat tgggcgtttt ttcgtaaaga aggaatttat tgatcatcac ctgcatccgg
     3181 taatctccct ggttggtgaa ccggccgatc atcatctcag gaaaatagtc gttgccatct
     3241 acttccacaa aataatcttc gttgggaaac gaataatcgg gataggtcac gatctttttc
     3301 ggaaaaaccc cgtcatcccc cacaatcagt acataagtcg gaggatattc ccagttatgg
     3361 taggcatcgg cgatatggtt cttgatgatg tcaggattat tggcgttggc gccgatatcg
     3421 gagaatctgg tgacatgcac gtcggttccg ctctgccgtt tccattcggc ataaacctgg
     3481 aaactattgt aaaactcatc aggcatgatg cacaggatca ggtcacgtcc gtcttccctc
     3541 ccgccgtact gctcattcag tacctcctgg taattgaaga tggtacttct gtaaactttt
     3601 ccgaaagaag gagcaatggg ccttttgagc gctgtccggg gatttattgc ct
//
LOCUS       DAOQXS010000322         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26489_length_3638_cov_4.399665, whole genome shotgun sequence.
ACCESSION   DAOQXS010000322 DAOQXS010000000
VERSION     DAOQXS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26489_length_3638_cov_4.399665"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKM34_09020"
     CDS             complement(<1..181)
                     /locus_tag="PKM34_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNQ83771.1"
                     /translation="MPEIKTTEIAILGAGAAGLTAAIRLGQLGIPCLLIDKGEFPRSK
                     ICGDGLSGKTLSALKM"
     gene            260..523
                     /locus_tag="PKM34_09025"
     CDS             260..523
                     /locus_tag="PKM34_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNQ83772.1"
                     /translation="METKYKSEFFVTGEELLGKVRELIHEGNVRKIIIKNEKGETYLE
                     IPVTIGVIGAILAPVFAAIGALAALAANFTIVVERKDDEPAAS"
     gene            complement(518..1270)
                     /gene="aroE"
                     /locus_tag="PKM34_09030"
     CDS             complement(518..1270)
                     /gene="aroE"
                     /locus_tag="PKM34_09030"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338490.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNQ83773.1"
                     /translation="MRIFGLIGYPLGHSYSKQYFDRKYLAENITDCRFENFPLNDISL
                     MNDLIINNPECAGFNVTIPYKSRIMEYLDWIEPGAREIGSVNCIKVEREGGQTIRKGF
                     NTDMPAFRETLRMFVPDAGISGALVLGSGGAAKSVCQALRETGIEPVIISRSAGMGQL
                     TYHSLSSEIIKKNRLIINTTPLGMWPETEAFPPLPYHLITSHHIFYDLIYNPEMTTFL
                     MKGAEAGAKIISGLHMFHIQAELSWKIWNSGL"
     gene            complement(1277..2689)
                     /locus_tag="PKM34_09035"
     CDS             complement(1277..2689)
                     /locus_tag="PKM34_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83774.1"
                     /translation="MESSFEHNETDLKALLERFEEMVRSDGAYFFDVEDLEDIVDYYL
                     DNQNLVKAKKAIDLAEAQHPGSTVFQLKKARYLILSEKTGKAISLLEEIEKIDPTNPD
                     IYMLKGSILSRLKKYDEAIGEYHRALFYSGDSEDIYNSIAFEYENAGNYDKAAEYLVR
                     ILEINPDNEAVIFELSFCYEITDNLEGAVDFLTQFLDRYPYNKIAWFNLGIAYNNLEL
                     FERAIEAYEFAIAIDERFSSAYFNLGNAYANMGEYRLSIKNYKEALKYEEPQALTYYY
                     IGECYEKLDRFPDAIQYYRLALEIDPALAEAWMGLGVCLDETGDPHTAARHMERALDL
                     EDDNAENWYIYGDLKYKLNDKEAAVRAFEKVSALDPMHSDIWLDLADIYLETGEMTKA
                     FDTLEEGIFQQPKNASLLYRMSSLLFRNGKQKQGLAVLSEGLETDFDKHSDLFEFAPE
                     LEQNRDVQELIQLHYNKMHN"
     gene            2800..>3638
                     /locus_tag="PKM34_09040"
     CDS             2800..>3638
                     /locus_tag="PKM34_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ83775.1"
                     /translation="MTLIKSISGIRGIVGDGLTPPEIVRFTSAYVNYIRQENAEKRLK
                     IVVGRDARVSGQMVSAMVCSILMSCGADVIDLGLATTPTVEMAVKSYGADGGIIITAS
                     HNPAQWNALKLLNEKGEFLSAAAGETILQLAERNDFGYMGYRDIGTCQQDNNYLVTHI
                     NKILALPLVNKIAIERAGFRIAFDAVNSVGGLALPALLKALGVKEIIPLNANPDGLFA
                     HNPEPLPEHLAGICSLVKQTGADAGFVVDPDVDRLAIITETGGFFGEEYTLVAVADYI
                     LKH"
BASE COUNT          942 a          865 c          855 g          976 t
ORIGIN      
        1 gcatttttaa tgcgctgagg gtttttccgc tcaggccgtc accgcaaatc tttgagcggg
       61 gaaattcccc cttatcgatt aacaggcacg gtattccaag ctggccaagc cggatggccg
      121 ccgtcaggcc cgctgcgccg gcccccagaa tagctatctc tgtcgtctta atttctggca
      181 tggaatttag tggttgccaa aattagataa aatcagccac gctgataatt tgaataattt
      241 tgaattaaat tttaaaaaga tggaaactaa atataaatcg gaattctttg tgacgggaga
      301 agagctcctg ggaaaagtca gggaacttat tcacgaaggc aatgtcagga aaatcattat
      361 caagaatgaa aagggggaaa cctacctgga gataccggtt accatcgggg tgatcggcgc
      421 gatactggcg cctgttttcg cggctattgg ggccctggcc gccctggccg ccaattttac
      481 cattgtggtc gagcggaaag acgacgaacc ggccgcctca taggccggaa ttccagatct
      541 tccagcttaa ttcagcctga atgtggaaca tatgaaggcc actgataatt tttgcccctg
      601 cttcagctcc tttcatcaga aatgttgtca tttcaggatt gtagatgagg tcataaaaaa
      661 tgtgatgtga agttattaag tggtagggaa ggggtggaaa tgcttctgtt tccggccaca
      721 tgccgagagg tgtcgtattg atgatcagcc tgtttttttt gatgatttcg gatgataaag
      781 aatgataggt aagttgaccc atacccgccg aacgtgaaat aatgaccggt tcaatgcccg
      841 tctccctgag cgcttggcag acagatttcg ccgccccacc tgatcccagg accagggcgc
      901 ctgaaatacc tgcatccggg acaaacatcc ggagtgtttc acgaaaagca ggcatatcgg
      961 tattaaaacc cttccggatg gtttgcccgc cttccctctc aactttaatg caattcaccg
     1021 atccgatctc ccgggcccca ggttctatcc aatccagata ttccattatt cgtgacttat
     1081 agggaatggt tacattaaaa ccggcacact ccggattgtt gataatcaga tcattcatta
     1141 acgaaatatc attaagtggg aaattttcaa acctgcaatc agttatattt tccgcaagat
     1201 atttacgatc aaaatattgt tttgaataag agtgaccgag ggggtaaccg atgaggccga
     1261 aaatcctcat gcgggatcag ttatgcattt tattgtaatg cagttggata agttcctgaa
     1321 catctctgtt ctgttccagt tccggggcaa attcgaacaa gtcggagtgt ttgtcgaaat
     1381 cggtctcaag tccttccgat aaaacagcca gtccctgctt ttgttttccg ttacggaaca
     1441 atagcgatga catccggtag agtaacgatg cgttcttagg ctgctggaaa atgccttctt
     1501 caagtgtgtc gaatgctttg gtcatttcac cggtttccag gtaaatatcg gccagatcaa
     1561 gccatatgtc actgtgcata gggtcaagtg cgctgacttt ttcaaaagcc cgaacggctg
     1621 cctccttgtc gttgagctta tatttcaggt caccgtaaat gtaccagttc tccgcgttgt
     1681 catcctccag gtcaagggcc cgttccatat gcctggccgc tgtgtgggga tcgccggttt
     1741 catcgaggca aacgcccagg cccatccagg cttccgctaa agccggatcg atctccagcg
     1801 ccagccggta atactggata gcatccggga agcgatccag cttttcataa cattccccaa
     1861 tatagtaata tgtcaaagcc tgtggctctt catatttcaa tgcctctttg taattcttta
     1921 tcgacagcct gtattccccc atatttgcat aagcattgcc caggttaaaa taggccgatg
     1981 aaaaccgctc gtcgatagca atggcaaatt cataggcttc gatggcccgc tcaaataatt
     2041 ccaggttatt ataggcaata cccaggttga accaggctat tttattgtat ggataacggt
     2101 caaggaactg cgtgaggaaa tcgacagctc cttccaggtt atcggtaatt tcataacaaa
     2161 acgataattc gaatataacc gcttcattat ccgggttgat ctccaggatt cggaccaggt
     2221 attcggccgc cttatcataa ttaccggcat tctcatattc gaaagcaatc gagttgtaga
     2281 tatcctccga atcgcccgaa tagaaaagcg cccggtgata ttccccgatt gcttcgtcat
     2341 actttttcag cctgctgaga atagaacctt tcagcatata gatatcggga ttggtaggat
     2401 cgatcttttc aatctcttca agcaacgata tggctttccc ggttttttcc gaaagaatca
     2461 ggtagcgggc tttcttgagc tggaaaaccg tggaaccggg atgctgtgct tccgcaaggt
     2521 ctatcgcttt tttcgctttg accagattct gattgtcgag ataatagtca actatatctt
     2581 cgaggtcttc cacatcaaaa aaataagccc catctgagcg gaccatttcc tcaaaccgct
     2641 ccagcaaggc tttcaggtct gtttcgttat gctcaaagga agattccatc aaaatactga
     2701 aaaatttgcc gcaaaaatac atattttctt tgtcctgccg acttgtcgtt ttcagcttca
     2761 atcacgctaa cttcattact tttgccaaaa atatcttgaa tgactctcat taaatccata
     2821 tccggcatcc ggggtattgt gggcgacggg cttactccac cggaaatagt caggtttaca
     2881 tcggcttatg ttaattacat ccggcaggag aatgcggaaa aacgcctgaa aatcgtcgta
     2941 ggccgtgatg ccagggtatc agggcaaatg gtttcagcca tggtttgcag tatacttatg
     3001 agctgcggcg ccgatgtcat tgacctgggg ctggcaacca cgccgactgt ggagatggct
     3061 gtaaaatctt acggagccga tggcggtatc atcattacag ccagccataa ccctgcacag
     3121 tggaatgctt tgaaattact gaacgaaaag ggcgaattcc tttcggctgc cgcaggcgaa
     3181 accatactgc aactggcaga aaggaacgac ttcggataca tgggataccg cgacatcggt
     3241 acctgtcagc aggataataa ttacctggta acacacatca acaaaatcct cgcattaccg
     3301 ctggtcaata aaatagctat cgagagagcc ggtttcagga tagcttttga tgccgtgaat
     3361 tctgtgggcg ggctggcgct tccggcttta ctcaaagcgt tgggcgtaaa ggagatcata
     3421 ccgctgaacg caaatcccga cgggcttttc gcccataatc ccgaaccgct tcccgaacac
     3481 ctggcaggga tctgcagtct cgttaaacaa accggtgcgg atgccggttt tgtggttgat
     3541 ccggatgtcg accggctggc tatcatcacg gagactggcg ggtttttcgg cgaggagtat
     3601 actttggtcg cagtggccga ctacatcctg aagcatga
//
LOCUS       DAOQXS010000323         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26541_length_3633_cov_3.978200, whole genome shotgun sequence.
ACCESSION   DAOQXS010000323 DAOQXS010000000
VERSION     DAOQXS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26541_length_3633_cov_3.978200"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PKM34_09045"
     CDS             <1..1277
                     /locus_tag="PKM34_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNQ83776.1"
                     /translation="TNGGVAPLDNSVWGTVVIGGETRSQCPFSATRQGLDGRASRGHV
                     DDYWIQSDNPGPDPFIVNGWTEHTYGDCTGDFMKTNQSTYGNVDGGTVFYNYVDGSPF
                     SDPGGGNNDGMYGLKLFYESRGYYVASHFNQYIMGYNGNGTGFTFADYKAEIDAGRPV
                     LIQVEGHTMLGMGYNDTGNTIYLHDTWDYTMHSMTWGGTYAGMLHYGVGVIRVYANPV
                     GPCASVIPINGTGAGNAKTYTGGGTGEWFQTGSNPCGYTSPGIEQVYSFTAPFTGTFN
                     IQVTAANGWVDYFWRAESCNPTGWTCISDIINPGAYGNLSWNSGTTYYILLDDEDNTA
                     GTHTFYINGVQTVPDNLTLQNITIASGQTECYGAAQTITTAGGGTTFTVQSGGTATLI
                     AGQNILMLPGTLLQSGSSVLGKITTNGTYCIP"
     gene            1341..2588
                     /locus_tag="PKM34_09050"
     CDS             1341..2588
                     /locus_tag="PKM34_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83777.1"
                     /translation="MVLFSGRKCLIVLGMHRSGTSAITGALSETGFEIGKNIMPPADE
                     NPKGFFENSSIVSLNDQILSELYVFWDDTLFIPDGWWKSEIFSGYKEKIRIILEDEYP
                     GDKPMLVKDPRMCVLLPLYLEVLQQMGTEPAFLVCVRHPLPVARSLGRRNNMPAEKAS
                     LLWADYQFKAELYSRGFSRLFVAYDEFLSDPLQVLYSIKDKLVPEMNIPDEVAGRIGS
                     FVDPALSNRFPESNRLYDFPYTGIANLYTIQQSSHLRDLKEDERVTTDGIRHQFENLN
                     RFFNGMPDQYRAALTVEFENGETIVLIAPIVFGTNTLEYPVSVQKKVKKLIFRPCNAK
                     TGIRWIEAEAYGPEENKIILAFTGTNATAKNAEKQVYIFETGLSKILFDFPEPFFASR
                     IIISIRYLVFGPAPCRKALWRKD"
     gene            complement(2585..3454)
                     /locus_tag="PKM34_09055"
     CDS             complement(2585..3454)
                     /locus_tag="PKM34_09055"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNQ83778.1"
                     /translation="MIGPIVIGGIGGSGTRVVAEILTCFGFYLGADLNGARDNLTFTL
                     LFKRPAWYIRNKDNFRHLETGLRILEKTMLTGKRLSLKEKFYLKRAVRDMSKYGHNRE
                     GQGKGEWALQRLPFIKNPVIPDPAVYTGWGWKEPNSHLLIPVMNNFFPGFKYIHTVRH
                     GLDMAYSSNQQQLFNWGPLFGVPLPVHENETPSASFRYWVEAHKRILALQKEIGRDRI
                     LILNFDRLCEDPERGIREILEFLGIQPSEELLARAAKLPVTPKTAGRWRQHPEDVFDE
                     NDLEFLRKMGFDG"
BASE COUNT          902 a          955 c          916 g          860 t
ORIGIN      
        1 cgacgaacgg aggcgtggca ccgctcgaca attccgtgtg gggaaccgtc gtcatcggcg
       61 gcgaaacacg gagccagtgc cccttcagcg ctacccggca agggctcgac ggaagggcat
      121 cccgcggcca tgtcgacgac tactggatcc aatcggataa ccccggcccc gaccccttca
      181 tcgtcaatgg atggaccgaa catacttacg gcgattgcac cggcgacttt atgaaaacaa
      241 atcagtcgac ttacgggaat gtggatggcg gcacggtttt ctacaattat gtggatggtt
      301 cccccttctc tgatcccggt ggcggaaata atgacgggat gtacgggctg aaacttttct
      361 atgaatccag aggttattat gtcgccagcc atttcaacca gtatattatg ggatataatg
      421 gcaatggtac cgggttcact tttgccgatt acaaagccga aattgatgcc ggaaggcccg
      481 tgctgatcca ggtcgaaggc catacgatgc tcggcatggg atacaacgat acgggaaata
      541 ccatctacct gcacgatacc tgggattaca ccatgcactc gatgacctgg ggaggcacct
      601 atgccggcat gctgcactat ggcgtcgggg tgatcagggt atatgcaaat ccggtcgggc
      661 cctgtgccag tgttattccg atcaatggca ccggtgccgg aaatgctaaa acttacaccg
      721 gcggcggaac cggcgaatgg tttcagaccg gttccaatcc ctgcggttac acttcgcccg
      781 ggatagaaca ggtttacagc tttaccgccc catttaccgg cacttttaat atccaggtaa
      841 cggcagccaa cggatgggtc gattatttct ggcgggcgga atcatgcaac ccaacgggct
      901 ggacctgcat ctccgacatt atcaaccccg gtgcttacgg aaatctttcg tggaacagcg
      961 gaacaaccta ctacatactg ctggatgatg aagacaatac cgccggaacg catacctttt
     1021 atataaacgg agtgcaaacg gttcccgaca accttacctt gcagaacatc accattgcca
     1081 gcggccagac cgaatgctac ggcgccgcgc agacgattac cactgccggc ggcggcacta
     1141 ccttcacggt tcagagcgga ggcaccgcca cactcatcgc cgggcagaat atcctgatgc
     1201 tgccgggaac gctattgcag tcgggcagca gcgtgctggg aaaaatcacg accaacggaa
     1261 cgtattgcat accataattc tgcgatccat aaggaaaatt gacgatttca tcctacattt
     1321 gtaaataatc acttttcctt atggtccttt tttccggaag aaaatgcctt atcgtgctgg
     1381 gcatgcaccg cagcggaact tccgcaataa ccggagcttt gtcagaaacc gggtttgaga
     1441 tagggaaaaa catcatgccc ccggccgatg aaaatcccaa agggtttttc gaaaatagca
     1501 gtatcgtcag cctgaacgac cagatcctgt cggaattgta tgttttctgg gatgatacct
     1561 tgtttatccc agatggctgg tggaaatcgg agatcttctc cgggtacaag gaaaaaatca
     1621 gaatcattct tgaggatgaa tatccgggag acaagccaat gctggttaag gatcccagga
     1681 tgtgtgtcct tttgcctctc tacctggaag ttctgcagca gatgggaacg gagcccgcat
     1741 tcctggtctg tgtccgccat cctctgccgg tggcccggtc attgggccgg agaaacaata
     1801 tgcctgccga aaaagcttcg ctcctgtggg ccgattacca gttcaaagcc gagctttatt
     1861 ccaggggatt ttcgaggctg ttcgttgcgt atgatgaatt cctgtcggat ccgctgcaag
     1921 tcctctattc aatcaaagat aagctggttc cggagatgaa tatcccggat gaagtggccg
     1981 gcaggatcgg ttcattcgtc gatccggcgc tctcaaaccg ctttcccgaa tccaatcgcc
     2041 tgtacgactt tccatacacc ggtattgcaa acctttatac tatccagcaa agctctcatc
     2101 tgcgtgatct aaaagaggat gagagggtta cgaccgatgg catcaggcat cagtttgaaa
     2161 accttaaccg atttttcaat gggatgccgg atcaataccg ggctgccctt accgttgaat
     2221 ttgagaatgg agaaaccatc gtattgattg cacccattgt tttcggcact aataccctgg
     2281 aatacccggt aagtgtacag aagaaggtta agaagctgat cttcaggccg tgtaatgcaa
     2341 agaccggtat ccggtggata gaagcggagg cctatggtcc ggaggagaat aaaataatac
     2401 tggcttttac aggaacgaat gcaacggcaa aaaatgcaga aaaacaggta tatatttttg
     2461 aaacaggcct ttcaaaaatt ttgtttgatt ttcctgaacc gttctttgcc agccggatca
     2521 tcatatcaat ccggtacctg gtcttcggac cggcgccctg ccgcaaggca ctgtggagaa
     2581 aggattagcc gtcaaacccc attttcctca aaaactccag gtcattttca tcaaaaacat
     2641 cctccggatg ttgcctccag cgtccggctg ttttcggtgt gaccggaagt tttgccgccc
     2701 gggccagtaa ctcctccgaa ggttgaatgc cgagaaattc caggatctcc ctgatccctc
     2761 tttccggatc ttcgcaaagc ctgtcgaagt taagaataag gatcctgtcg cgtccgatct
     2821 ctttctgcag ggcgagaatc cgcttatgcg cctccaccca gtagcggaag gaggcagatg
     2881 gagtctcatt ttcatgaacc ggtaacggca ccccgaacag cggcccccag ttgaaaagct
     2941 gttgctggtt gctgctgtag gccatgtcga gaccgtgcct cacggtgtga atatatttga
     3001 atcccgggaa gaaattgttc attacaggaa tgagaaggtg gctgttgggc tctttccagc
     3061 cccagccggt gtaaaccgca ggatcaggaa tgaccggatt tttgatgaag ggtaatcgct
     3121 ggagcgccca ctcccctttc ccctgccctt ctcgattgtg gccgtatttc gacatgtcgc
     3181 gcacggcacg cttaaggtag aatttctcct tcagcgacag ccgtttaccg gtaagcatgg
     3241 tcttttccag gatccgcagg ccggtttcca ggtggcggaa attgtccttg ttcctgatat
     3301 accaggcggg ccgcttgaaa agcagcgtaa aggtcaggtt atccctggcg ccgttgagat
     3361 cagcgccgag gtaaaaacca aagcaagtaa ggatttcggc gacaaccctg gtgccgctgc
     3421 cgccaattcc gccgataaca attgggccga tcataggatt gtttatttcc agggatgaaa
     3481 ttaggaaaaa tctgatgcgg gactattctg cttaaacgtc attttataac actccggcaa
     3541 tttacctcac cattactttc cccgaatacc tcctctcatt cctatccgtt accaccacca
     3601 catacatccc cggcggataa cccgacacat cca
//
LOCUS       DAOQXS010000324         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26741_length_3614_cov_3.774937, whole genome shotgun sequence.
ACCESSION   DAOQXS010000324 DAOQXS010000000
VERSION     DAOQXS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26741_length_3614_cov_3.774937"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PKM34_09060"
     CDS             complement(<1..1311)
                     /locus_tag="PKM34_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNQ83779.1"
                     /translation="MKKFFFIILVAILAPFSLIADWIPFDSKSTPHTPPGVTILSDDQ
                     NSTVIKIELGGIDLSDFQAEGKNYQVADLLTGTFTAEPGYPEVPYLANILAVPDRAAI
                     SLEVVETGETRIFSNILLPPSRISPVEGSPEPPYMENAGIYQSGILFPAEFAKADPPA
                     IFRDFRIARISVFPVHYLPAKKELQVVSSITVRVNYAAGEAINPKITAKRSIAPSYGQ
                     LYRSLILNYPQVLESCYGGREEGRELILCIMPDIFAESFQVYADWKRQSGIDVHVTKF
                     SDIGANASNPDIIKAHITDAYHNWEYPPTYVLLVGDDGVCPKKIVVYPDYSFPNEDFF
                     VEVDGNDYFPEMMIGRFTNEAEFRMQVMINKLLKYEKEPFVQDASWLKKGTCCSNNAY
                     PSQLETKRFAYHKMMDEGNFLAVDTLMSNGTGGSGCTVDLQDVTA"
     gene            complement(1564..>3614)
                     /locus_tag="PKM34_09065"
     CDS             complement(1564..>3614)
                     /locus_tag="PKM34_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNQ83780.1"
                     /translation="NCDADFFSWSTGQFGVSSITVSDSGSYWVRGRFNNSGCMGYDTI
                     HIGFHQTTIIDETNLNITPTTCNGASGSITGLTAIGMPPFFYGWKDLSEVEVGTDIEV
                     FDLPAGQYYLTITDANGCETTSDIYTIEDAGNLQVLDVELIQPHCGRPDGEIVVHGFN
                     PSGSALQYSINDGDSYQADSVFSGLVDTSYVVRVTDGGGCFGFYIDNPVVLEDIPGPQ
                     VVEVNVTDETDFLGNGAIEIIASGSTPVIYYSIDSGATYQSNNGTFNNLGSGIYDLLV
                     WDENGCDTAFTVEIQNNILTWLHAVTGEEGICEASTALIPVNVDNFNNVAEFHLKLGY
                     NADNIECEGFANVNVQLLDSLTGWVDQATGEIHLTWNGEFPLTFSQSELATDLVFTTK
                     NPGQGELSWYTGETESYFTNSSGNLIPAEFSTGQVTIYDPPEIILDQSKTVCTGQFAN
                     LMSIAVGNQPPITYRWIYPAGDSTSTDPLFFSITPADAGLYTLLATDRVGCTDQKSIQ
                     LIVSENPVAAFHGTDTLEMHMGDVLDAGEGMASYRWNTADSMQSIVIHAEGKYQVEME
                     SQIGCTGRDSIYIKIIEDEIPPESHIFIPNAFSPNGDGVNDVFVMKCDGLSIVDCRLS
                     IFDRWGGEIFSGDGISSGWDGRKNGKDCPGGVYVYKIVFSLDGVPGSRERVGTVMLVR
                     "
BASE COUNT          945 a          921 c          820 g          928 t
ORIGIN      
        1 cgccgtaaca tcctgcaggt cgacggtaca gccagagccg cccgtcccgt tgctcatcag
       61 ggtatcgacc gcgaggaagt tgccttcatc catcatttta tgataagcaa agcgctttgt
      121 ttccaattgc gatgggtagg cattgtttga acagcaggtc ccctttttca accaggaagc
      181 atcctgcaca aaaggctcct tctcatactt taacaactta ttgatcatga cctgcatccg
      241 gaattccgct tcgttggtga accgtccgat catcatttcc gggaaataat cattaccatc
      301 aacttccacg aaaaagtcct cattcggaaa ggaataatcg ggataaacca cgatcttctt
      361 cgggcaaacg ccgtcgtcgc cgaccagaag cacgtacgtc gggggatatt cccagttatg
      421 gtaagcatcg gtgatatggg ccttgataat atcgggattg ctggcatttg caccgatatc
      481 tgaaaatttc gtcacatgca catcgatccc gctctggcgt ttccagtcgg cataaacctg
      541 gaaactctcc gcgaatatat cgggcatgat gcaaaggatc agctcgcggc cttcttccct
      601 gccaccatag caggattcaa gcacctgcgg ataattcaga ataaggctgc ggtacaattg
      661 tccgtaggat ggagcaatgc tccttttcgc cgtgatcttc gggtttatgg cctcccctgc
      721 cgcataattc accctgacag tgattgatga aaccacctgc agttcctttt ttgcgggcag
      781 gtaatgtacc gggaaaactg aaatacgggc gatccggaaa tccctgaaga tggccggcgg
      841 gtcggctttt gcaaactctg ccgggaaaag tataccggac tggtatatcc ctgcattctc
      901 catgtaaggg ggttccgggc ttccttcgac cgggctaatc cgcgaggggg gcaacaaaat
      961 attgctgaaa atgcgggttt cgccggtctc aacgacttcc agagaaattg ccgccctgtc
     1021 gggaacagcc agtatattgg ccaggtaagg cacctccgga tagcctggtt ctgcggtaaa
     1081 ggttcccgtc agcaagtcgg cgacctggta atttttccct tctgcctgaa aatccgaaag
     1141 gtcaattccg cccagttcga ttttgatcac cgtgctgttc tggtcatcgc tcaggatggt
     1201 cactccgggt ggggtgtgag gggtactttt gctgtcaaaa gggatccaat ctgcgatcag
     1261 tgaaaatggc gccagtatag caaccagaat gatgaaaaaa aactttttca taataattct
     1321 tctgaaatta attcgattag tccggctaac gaacgagttt gcatctgttt gttcagggag
     1381 gaatattaat ttatccggat cgtaaaatta ttattaattg aaatgcggag gtcacaggat
     1441 gcacaatttt taaggataag tcaaagggat gagagaaagc aaattaattt gaagattagt
     1501 caatttggaa attagctaat tgtctcattt ccaaatttcc aaatttccaa atttccaaat
     1561 taatcatctt accagcatca ctgttcccac tctttcccgg cttccgggaa ccccatccaa
     1621 tgaaaacacg atcttataaa catacacgcc accagggcag tcttttccgt tcttcctgcc
     1681 atcccatccg gatgagatgc catctcccga aaatatttcc ccaccccacc tatcgaaaat
     1741 cgacaatcga caatcgacaa tcgacaatcc atcacacttc atcacaaaca catcattcac
     1801 cccatcgcca ttcggactaa acgcatttgg gataaaaata tgcgattccg gtggtatttc
     1861 gtcttctatt attttgatat atatggaatc tcgaccggtg cacccaattt gagactccat
     1921 ctcaacctgg tactttcctt cagcatggat cacaatgctt tgcatcgagt cagcggtgtt
     1981 ccaacgatag gacgccattc cttcccctgc atccaaaaca tctcccatat gcatttccag
     2041 cgtgtcggtt ccatgaaaag ccgcaacggg attttcgctg acgatcagtt ggatactttt
     2101 ctggtctgta cagccaacgc ggtctgtggc gaggagcgtg tagaggccgg cgtcagcggg
     2161 ggttatgctg aagaatagcg ggtcggtgct ggtgctatcg cccgctggat aaatccagcg
     2221 gtaagtgatc ggaggctggt tgccgacggc aatgctcatc aggttggcaa actgcccggt
     2281 gcaaacggtt ttgctctgat ccaggatgat ctccggcgga tcgtagatcg tgacctgccc
     2341 ggtactgaac tcggccggga tgagattgcc gctgctgttg gtgaaatacg actccgtttc
     2401 gcccgtgtac caggaaagct ctccctgccc cgggtttttc gtggtgaaaa ccaggtcggt
     2461 tgcgagttcc gactgactga aagtcagtgg aaattcacca ttccaggtca gatgaatctc
     2521 tcccgtggcc tgatcaaccc aaccggtaag gctatccaac aattgaacat taacattagc
     2581 aaagccttcg cattcaatgt tatcggcatt atatcccagc ttcaggtgaa attcagctac
     2641 attattaaaa ttgtccacat taacgggaat caatgctgta gaagcttcgc atattccttc
     2701 ttcgccggta accgcatgca accatgtcag aatattattc tgaatttcaa cagtaaacgc
     2761 ggtatcacat ccattttcat cccaaactag taaatcgtaa atcccagatc ctagattgtt
     2821 aaaggttcca ttgttcgatt gataagtcgc tccgctatcg atcgaataat aaatcaccgg
     2881 agtagagcca gaggcaatga tttcaatcgc tccattcccc aaaaaatccg tttcatcggt
     2941 aacattcact tcaacaacct gcgggccggg gatatcttcc aaaacaaccg gattatcaat
     3001 gtaaaatcca aagcacccac ctccatctgt aacccgcacc acgtaactcg tatccaccag
     3061 cccggagaac acactgtcag cctggtatga atcgccatca ttgatcgaat actgcaatgc
     3121 agaacctgac ggattaaatc catgaacaac gatctctcca tcgggcctgc cgcaatgagg
     3181 ctggatgagt tcaacatcca gaacctgcag gttgccggca tcttcaatgg tgtatatatc
     3241 ggatgttgtt tcgcaaccat ttgcatcagt gatagttaaa taatactgcc cggcaggaag
     3301 atcaaagact tcaatatcag taccaacctc gacctcggag aggtccttcc atccgtagaa
     3361 aaacggtggc ataccaatcg ccgtcagccc ggtaattgag ccgcttgccc cgttgcaggt
     3421 ggtgggggta atattcagat tggtttcgtc gatgattgtt gtttgatgga atccaatgtg
     3481 aatggtatca tagcccatgc agcctgagtt gttgaaccgt ccgcggaccc aatagctgcc
     3541 tgaatctgaa acggtaatcg atgaaacgcc gaactggccg gttgaccagg agaagaaatc
     3601 ggcatcgcag tttg
//
LOCUS       DAOQXS010000325         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26753_length_3613_cov_4.127600, whole genome shotgun sequence.
ACCESSION   DAOQXS010000325 DAOQXS010000000
VERSION     DAOQXS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26753_length_3613_cov_4.127600"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1315
                     /gene="tyrS"
                     /locus_tag="PKM34_09070"
     CDS             38..1315
                     /gene="tyrS"
                     /locus_tag="PKM34_09070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ83781.1"
                     /translation="MNFIEELRWRGMIHDMTPETEELFKKDEMVVGYVGIDPTADSLH
                     IGHLVSIMMLKHLQLAGHKPLALVGGATGMIGDPSGKSEERNLLSEEIIRHNEHCIKR
                     QLEKLLEFGNKPNAAEMVNNYDWMKEFTFLGFIRDVGKHITVNYMMAKDSVKKRLETG
                     ISFTEFTYQLVQGYDFLYLNEHKNCKLQMGGSDQWGNITTGTELIRRKNGGDAYALTC
                     PLITKADGGKFGKTETGNVWLDPVRTSPYKFYQFWLNTSDEDAEKYIRIFTLFTREEI
                     DEMIARHKEAPHQRELQKALAKDLTVRVHSEADYQAAIDASAILFGAGTTETLKGLSE
                     DLFLSVFEGVPQSEVSKVKLEQGIPLLEFLADETGIFPSRGEARRTVKENALSINKMK
                     VVEDKMVGVGDLLNEKYILVQKGKKNYFLVVAV"
     gene            1822..>3613
                     /locus_tag="PKM34_09075"
     CDS             1822..>3613
                     /locus_tag="PKM34_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ83782.1"
                     /translation="MKNFHLFRFIANSLKNWICTGLGICLVATFNVTSAQTVYQAGKY
                     SLLLENGMYYDISGPDTTKVTTNHMAIKLREGISENERSEFLSRHPLSYSGMGTTTSV
                     IIFNIDSLANFVNLIENIANDPDVELYDINHLVPICDTGKPNDGHDQVDYLLLNSYPN
                     LLWPYINTNLFEAWNFENGDSSIIVAEIDNGIWIDHPDISYGFDNYSNLWNNHDEING
                     ISNYDDDGNGFEDDFHGWNFTEGNGDLMHDNIFADKGTHGTIMSGIIAAKTNNELGIW
                     GTAGGYSTPGIRILPVKCNNLEDPNLLETDYIIAGIDYAIALGARVINMPFAGADFPG
                     IHNKISEYFNQGNVVFLAATGNDWQDDIDGEVYYPAKYEEVIGVGATECNPQDLNSLE
                     TRWILGHSWSSQYGVGIDICAPGSPFTTDFIWDGNQYSPTYNYYTIEDNGSTSRAVSF
                     ASGVVALILSANPCLTNEEVMDTLRNTAYKFPYPGLEWEYGWNKYYGYGRIDALKALQ
                     PVIIPPIEENTTWNSDMKIYNEINIENGAELTIQNCTISMSSWAKIIVKPGGTLILNN
                     CTLTGICNSIMWPGIEVWGYPNAPQFKYPLL"
BASE COUNT         1055 a          746 c          834 g          978 t
ORIGIN      
        1 taacgctttt tcctaccttt gcataaactt taggcttatg aactttatcg aagaactccg
       61 ctggcgcggg atgatccacg acatgacgcc cgaaacggaa gaactgttta aaaaggacga
      121 aatggtggtt ggttatgtgg gtatcgatcc gacggccgat tcgcttcata tcggccacct
      181 ggtgagcatc atgatgctga agcacctgca gcttgccggc cataagccgc tggcgctggt
      241 cggaggggca acgggcatga tcggcgaccc atcggggaag tcggaagaaa ggaatctgct
      301 tagcgaagag atcatccgtc ataacgaaca ttgtatcaaa agacagcttg aaaagcttct
      361 cgagttcggt aacaagccga atgccgccga aatggtcaac aactacgact ggatgaaaga
      421 gttcaccttc ctgggtttta tccgcgatgt cggcaagcat attacagtca attatatgat
      481 ggcgaaggat tcggtaaaga agaggctcga aacagggatc tcgttcaccg aattcaccta
      541 ccagctcgta cagggatatg attttctcta cctcaacgag cataagaact gcaaactgca
      601 gatgggcggc tccgaccagt ggggcaacat caccacgggc accgagctga tccggcgcaa
      661 gaacggcggc gacgcctatg ccctcacctg cccgctgatc accaaagccg acggcggcaa
      721 gttcgggaag accgaaaccg gcaacgtatg gctcgatccg gtacgcacct ccccatacaa
      781 attctaccag ttctggctga atacttcgga tgaggatgca gagaagtata tccggatctt
      841 tacgttgttt acgcgtgaag agatcgatga aatgatcgca cgccacaaag aggcaccgca
      901 ccagcgcgag ttgcagaaag cgctggcaaa ggaccttacg gtgagggtgc attccgaagc
      961 cgattaccag gctgcaatcg atgcgtcggc gatcctgttt ggcgccggca cgaccgaaac
     1021 attgaaaggc ctctccgagg atcttttcct ctccgttttt gaaggggttc cgcaaagtga
     1081 agtatctaaa gttaaactcg aacaaggcat cccgctcctg gagttcctgg cggatgagac
     1141 cggaattttc ccgtcccggg gcgaggcgcg gcggacggta aaagagaatg cgttatcgat
     1201 caataaaatg aaggttgtgg aagataaaat ggttggcgtg ggtgatcttt tgaatgaaaa
     1261 gtatatcctg gttcagaaag ggaagaagaa ttattttctc gttgtagctg tttgattttt
     1321 aaccacatag acacaacttg tcccgaaacc tcgggatagg gcacaatagg ttacacatag
     1381 atctgttgtg ctttctattg tgttcaaagg gtcagtgtgt tgaatagcga cgtctcatac
     1441 ggtgtgccct ggtaaaggaa gagataagga ttgagactcc atctgcccga aatgagatgc
     1501 catctcgatt ttgagatgga gtctccttgc aatcgagatg ccatctcatc caaaccagat
     1561 ggcgtctcaa actaaatagt tatcaaccgc acttgacttt ccattcaaat cagcttatct
     1621 ttaagccgat tagtccactt ccgcattttt ctatcaacaa aattaatcgc catgtctgct
     1681 tttggcactt ccgtagagag agagagagag agagagagag agagtttaaa ttctcacatt
     1741 cagcaggttt tttgttattc tgtcaacttt cgaatatcct atttattcct ttctgtttat
     1801 taaccgttaa tccgaataat tatgaaaaat tttcatttgt ttcgatttat tgccaatagc
     1861 ttaaaaaatt ggatctgcac cggacttgga atttgtcttg ttgcgacatt caatgtgact
     1921 agtgcacaaa ccgtgtatca ggcaggtaaa tattctttac ttttagagaa tggaatgtat
     1981 tatgacattt ccggtcccga tacaactaaa gtgacgacaa accatatggc gatcaaatta
     2041 cgtgagggca tttcagaaaa tgaacgttcg gaattccttt cacgtcatcc tctttcctat
     2101 tcaggaatgg gtacgactac cagtgtaata atattcaaca tcgatagcct ggcaaatttt
     2161 gtcaatctta ttgaaaatat tgcgaatgat ccggatgtgg aattatatga tattaatcac
     2221 ctggtcccaa tttgcgatac cggaaaacca aatgatggtc atgatcaggt tgattatttg
     2281 ctgctgaaca gctatcctaa tttactttgg ccatatataa atacaaatct ctttgaagcc
     2341 tggaatttcg aaaatggcga ctccagtata attgtagctg aaattgataa tggcatctgg
     2401 attgatcatc cagatatttc atatggattc gataattatt caaacctatg gaacaatcat
     2461 gatgaaatca acgggatatc gaattatgac gatgatggca atggattcga ggatgacttt
     2521 catggatgga attttacaga aggcaatggt gacttgatgc atgataacat ttttgctgat
     2581 aaagggacgc atggtacgat aatgtcagga ataattgcag ccaagacaaa taatgaactg
     2641 ggaatatggg gcactgccgg cgggtattcg actcctggta ttaggatttt accggttaaa
     2701 tgcaacaatc ttgaagaccc aaatcttctc gaaaccgatt atattattgc tggcattgat
     2761 tatgcaattg ccctgggagc aagagtaatt aatatgccat ttgccggtgc tgattttcct
     2821 ggtattcaca acaaaatatc tgagtacttt aatcaaggaa atgttgtttt ccttgctgca
     2881 accggaaatg actggcaaga tgatattgat ggagaagtat attatccggc aaagtatgaa
     2941 gaagtaattg gggtcggcgc tactgagtgc aaccctcaag acctgaatag tcttgaaaca
     3001 cggtggattc ttggacattc ctggtcctca caatatggag ttggaattga tatttgtgca
     3061 ccgggatcac cctttacaac tgatttcata tgggatggaa accaatattc acctacttat
     3121 aattattata ccattgaaga taatggttcc acttcaagag cagtatcatt tgcttcaggt
     3181 gtggtcgcat taattctttc cgcaaatccg tgtctcacca acgaagaagt tatggataca
     3241 ttaagaaaca ctgcttataa gtttccttat ccgggtcttg agtgggaata tggctggaac
     3301 aaatattatg gatatggaag gatagacgca ctaaaagctc tgcagcccgt tatcattcct
     3361 cccatcgaag aaaacacaac ctggaacagc gacatgaaga tttataatga aattaatatc
     3421 gaaaatggag cagaactgac cattcaaaat tgcaccattt caatgagtag ttgggcaaag
     3481 atcattgtta aaccaggagg gaccttgatt ctaaataatt gcactttaac cggtatatgc
     3541 aatagcatta tgtggccagg catcgaagtc tggggttatc cgaatgctcc gcaattcaag
     3601 tacccgcttt tac
//
LOCUS       DAOQXS010000326         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26888_length_3601_cov_3.909757, whole genome shotgun sequence.
ACCESSION   DAOQXS010000326 DAOQXS010000000
VERSION     DAOQXS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26888_length_3601_cov_3.909757"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKM34_09080"
     CDS             complement(<1..405)
                     /locus_tag="PKM34_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ83783.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALLDEFLRHDPESKVACETL
                     VTTGLVVTAGEVRTKGYVSIDEVVRNTIKKIGYTKAEFKFDADSCGVISTIHGQSDDI
                     YRGVERGSKTEEEKYRQGAGDQGMMFGFASTEM"
     gene            complement(650..1561)
                     /gene="gldA"
                     /locus_tag="PKM34_09085"
     CDS             complement(650..1561)
                     /gene="gldA"
                     /locus_tag="PKM34_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNQ83784.1"
                     /translation="MSIEVSNVIKIYDTQKALDNVSMSVKPGEIVGLLGPNGAGKSTL
                     MKIITCFIPPTEGAATICGYDIFENSLKVREKVGYLPEHNPLYLDMYVGEYLEFVAGI
                     HKIKSNVKSRIGEMIELTGLTDERKKKIGALSKGYRQRVGLAQALIHDPEVLILDEPT
                     SGLDPNQISEIRHLIRSVGATKTVMLSTHIMQEVEAICDRAIIIHKGRIVADDATRNL
                     TTGIKNRNRITVEFSGKIDSQDFKRISGITGIERKSEKVWALETAEGRDIREELFSLA
                     VSKGIAILSMNREELRLEEVFQILTQG"
     gene            1692..>3601
                     /locus_tag="PKM34_09090"
     CDS             1692..>3601
                     /locus_tag="PKM34_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83785.1"
                     /translation="MFLIIHCTVVNGQNESIIISSDSINPLTDNTYFNASLFGHLPFT
                     GTSDIFQLNPALYRNSAAGTFLIDGFSTSGDYTLFDGIPMDFIEEMPLRLIGEVKFND
                     FHDFLTCNNTITGYARLEPLRQSDSLTIRLEPSTRIIHGQFNDADLQLIISGPLIKQT
                     PGKNNSTSLNFTLAGRLFSASDPNPSYIGRDQASADYLDYLADESLRPSEFSGTYPNA
                     CFTHATNAVSSYFNSNAAKKGFSLYGSVSAEAGNGMFLKLGTYTVKKKETIPVYDNFF
                     FNQDKNPDQSTLYSNNYITLGQILKSGRSTRFTYQVQGNYSIQKNKIEDPDYGDDFFK
                     YGYVGKFETFKRPTYELGSIQVGDTYYEQALILNSWDYDTLVDFSPGEINPGLAAYTS
                     SYFDIFVGEPDNHYGNKDQIQLGGGILNGQNPDKVYGLWNNTGTVYNYYSKEDNRRIN
                     IAASGMLELDNHKIKIGFQFRRDSYSSYQANPVGLWGLMRSMTNFHINELDLSNPEVH
                     EGGLWDSVFFYRKYDAASQHYFDRNLREALGLDPDGTDFIDIDSYDFITSTINYFDKE
                     GLKHTIDVGKELFSLDMFSADELLNDGIASFVTYSGYDHLGNKNGNKVAFEDFFTATD
                     GEGEYIRPVGAYT"
BASE COUNT          907 a          846 c          779 g         1069 t
ORIGIN      
        1 catctcggta ctggcaaatc cgaacatcat cccctggtcg cctgctcctt gcctgtattt
       61 ttcctcttcc gttttcgatc ctctctccac gccccggtag atatcgtccg actgcccgtg
      121 gatcgtcgag atcaccccgc acgaatctgc gtcgaatttg aattctgcct ttgtataacc
      181 tatctttttt attgtattcc tgactacttc atcgatactg acataccctt tggtgcgcac
      241 ctcgccggcc gtcacaacca ggcccgtcgt taccagtgtt tcacaggcca ccttcgattc
      301 gggatcgtga cgtaagaact catccagcag ggcgtctgat atctggtcgg ctaccttgtc
      361 gggatgacct tccgacaccg attccgaagt aaataaatag ctcattatat cttatttttt
      421 aattatggaa ttagaggctg tctcaaaagt ctgatatttc gatttgattg cctgaaaaca
      481 gttcgtgctc caacgactaa agccgttggt tccgaatgtt ttatgagggt tggaaccgac
      541 ggcttcagtc gtcggactac ctaaaaaaag tgaatatata gccttttgag acaccctctt
      601 gcacattatt tttttaaggg cacaaatcta tgaataaaag caatatgaat cagccttgag
      661 tcagaatctg gaaaacctct tccagccgca gctcttcccg gttcatggaa agtatggcga
      721 tgcccttgct tacggccagg ctgaacaatt cctcgcggat atctcttcct tcggccgttt
      781 ccagagccca gactttttcg ctttttcttt cgatgccggt aatcccgctt atccgtttaa
      841 aatcctggct gtcgatttta ccgctgaact ctacggtgat ccggttcctg tttttaatgc
      901 cggtggtgag gttcctggtt gcatcgtcgg ccacgatcct gcctttatgg atgatgatgg
      961 cccggtcgca gatcgcttcc acctcctgca tgatgtgcgt cgacagcatc accgtttttg
     1021 tcgccccgac cgaccggatc aggtggcgta tctcgctgat ctggttggga tcaaggcccg
     1081 acgtcggctc gtcgaggatg agcacctccg ggtcgtggat cagcgcctgc gccagcccga
     1141 cgcgctggcg gtaccctttc gacaaagccc cgatcttctt cttccgctca tcggtcagac
     1201 ccgtcagttc gatcatttca ccgatcctgg actttacatt gcttttgatc ttgtggattc
     1261 cggcgacgaa ttccagatat tcgccgacat acatgtcgag atagagcggg ttgtgttccg
     1321 gcaggtagcc gactttttcc ctcactttca gcgaattctc gaaaatatcg tacccgcaga
     1381 tggtagcagc cccttccgtc ggcgggataa agcaggtaat gatcttcatc agcgtggatt
     1441 tcccggcgcc gttcggaccg agcagtccga caatctcacc gggtttgaca ctcatggaaa
     1501 cattgtccag ggctttctgc gtatcgtaaa ttttaattac attgctgact tcaatcgaca
     1561 tattgctttt tcgaataggg atgccgaaat tagtaaaata tttatattta tgctcttgaa
     1621 attattaacc ttttccttta agttctttcg gttttatgaa aaaaatcaaa tttattggat
     1681 cgattttgat catgtttttg atcattcatt gcacagttgt taacggtcaa aatgaatcaa
     1741 taattatttc atccgatagc ataaatccgt taacagataa tacttacttc aatgccagct
     1801 tattcgggca tcttcctttt accggaacgt ctgatatttt tcagttaaat ccggctcttt
     1861 accggaacag cgccgccgga acctttttaa tagatggatt cagcacttca ggcgattata
     1921 ccttatttga cgggatcccg atggatttta tcgaagaaat gccgctcaga ctgatcggtg
     1981 aagttaagtt taatgatttt catgattttc tcacctgcaa caataccatc accggttatg
     2041 cccggcttga gccgctccgg caatccgaca gcctgaccat ccgcctggaa ccatccaccc
     2101 gcataattca tgggcagttc aatgacgcag acctgcagtt aatcatttcc ggtccgttaa
     2161 tcaaacaaac acccggaaaa aacaattcca cttcactgaa ctttacgctg gcaggccgct
     2221 tgttttctgc atctgatcca aacccttcgt atatcgggag agaccaggca tctgcagact
     2281 accttgacta tctggcggat gaatcccttc gcccttctga atttagtggt acttacccaa
     2341 acgcttgttt cacccatgcc accaatgctg tttcgtctta ttttaattca aatgctgcta
     2401 aaaaaggatt ttcgctatat ggttctgtga gtgcggaagc tggaaatggg atgtttctga
     2461 aattgggcac atacacggtg aaaaaaaagg aaaccattcc ggtctatgac aattttttct
     2521 ttaaccagga taagaatcca gatcaatcaa ccttatattc aaacaattac attactctcg
     2581 gtcagattct taaatccggg agatcaaccc ggtttaccta tcaggtgcaa ggtaattact
     2641 cgattcagaa gaacaagatt gaagatcccg actatggcga tgattttttc aaatacggat
     2701 atgttggaaa atttgagaca ttcaaaaggc caacttatga gttaggctct atccaggtcg
     2761 gtgatacgta ttatgaacag gcattgatac tgaattcctg ggactacgat accctggtcg
     2821 atttttcacc gggtgaaata aatccgggat tagccgctta tacttcgtcg tattttgaca
     2881 tctttgttgg tgaaccggat aatcactacg gaaacaaaga ccagatccaa ttgggaggag
     2941 ggatactgaa tggtcagaat cctgacaagg tttatggtct gtggaataat accggcactg
     3001 tttataatta ttattcaaaa gaggataaca ggaggatcaa tattgctgcg agtggtatgc
     3061 tggagcttga caaccataag ataaaaattg ggtttcagtt ccgaagagac agttattctt
     3121 cctatcaggc caacccggtg ggtctgtggg gtttaatgcg ctctatgacc aattttcata
     3181 tcaacgaact cgatttgtca aatcctgaag tccatgaagg tggactttgg gactccgttt
     3241 tcttctacag gaaatatgat gcagccagcc aacattactt tgataggaac ctgagggaag
     3301 cgcttgggct tgacccggac ggaacagatt ttatcgatat cgattcctat gatttcatca
     3361 caagtacaat aaattatttt gacaaagaag gtttaaaaca cacgattgat gtcggaaagg
     3421 aattgttttc cctggatatg ttttctgctg atgagctgtt gaacgatggc attgcttcct
     3481 ttgttactta cagcggatat gatcatttag gcaataagaa cgggaataaa gttgcttttg
     3541 aggatttttt cacagctacg gatggggaag gagaatatat ccgtccggtt ggggcctata
     3601 c
//
LOCUS       DAOQXS010000327         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26901_length_3599_cov_3.939052, whole genome shotgun sequence.
ACCESSION   DAOQXS010000327 DAOQXS010000000
VERSION     DAOQXS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26901_length_3599_cov_3.939052"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..2212
                     /locus_tag="PKM34_09095"
     CDS             194..2212
                     /locus_tag="PKM34_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83786.1"
                     /translation="MKSKDQQQPPRRQKNRVPGNSLFSGRDRWRITGALLVILLISLL
                     SYLPVFHNELLTYDDQAYIQNNPMVYSMDLKEIFTQNVKSNYHPVTVLVLALEYRMFG
                     LDPTGYHVFNLFLHLINTLLVFYALYLLTRKAGIALVASLLFGVHPLHVESVAWVAEL
                     KDLLYTLFFLSSYIFYLKYLDDKSKKSYALALGLFLVSLLSKAMAASLPLVLILTDYF
                     KGRKIDRKSLIEKIPFFLFALVFGIVAIIAQKSTGAAGIVDLNIFQRIVFACYGFISY
                     LFKLVVPLNLSAYYSYPIGSGGTIPLLYYIFPVLVAGLVVLVYFSLRFTKKVIFAAGF
                     FLFTVFFVLQLLPVGGAVMADRYAYIPSIGIFYLAGEGFYYLWNRKQKLVPVILLSAF
                     TIFFAITTYSRSSVWKTDLSLWTDVIEHDPSVKLAYINRGNAFKNERKYDRAVADFSK
                     AMEIDPEYALAYFNRGSAYEDAKKADLALADYNKALELDPELAQAFNNRGNLFAGQNK
                     TELALADFEQAIRLDPGYSQAYNNRGTLYANANQPDLALADFTKAIELNPNNSKAYNN
                     RANIAVNRKKYDQAIEDFSRAIKLNPTFCQPYYNRGSVLSYLKKYDQAIADFTQAIAI
                     DANFMQAYFARGQAEYYSGKKAAACRDFRSAANLGFKPAADALLQLCN"
     gene            complement(2192..>3599)
                     /locus_tag="PKM34_09100"
     CDS             complement(2192..>3599)
                     /locus_tag="PKM34_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNQ83787.1"
                     /translation="RLIEKLPEPAAILLSYQDHPLMQDLSAQAIFGGTDINGRLPVTA
                     GNNYPAGTGKTTSSMRLKYTIPEELALDRTSLAPIDSIISDAISKKAFPGCQVFAAND
                     GKVFFSKSYGYHTYTNEIPVKNSDIYDIASLTKIAATTLAVMRLADEGKIDVDRKVET
                     YLPDFANTDKGNIVVREMMAHQARLRPWIPFYKATLKDGKPDSAFYRSTRSDKFPVRV
                     AENLFLTEAFDKIIFDSIKASSLLKSSQYKYSDLGFILLTPIIKNLTGLRLDSYAGKE
                     FYLPLGLSTTGYNPRERFDIQRIPPTENDTVFRRQLIRGDVHDQCAAMIGGVSGHAGL
                     FSNANDLGIIAQMLLNRGIYGGNQYLSEEIIGEFTRMQFPLNDNRRGLGFDRPLPVYA
                     EEGPCCRSASQSSYGHSGFTGTYIWVDPESELTFVFLSNRVYPNAENNKLSAMNIRPK
                     IHQVFYDALEKRSVTKLK"
BASE COUNT          863 a          868 c          867 g         1001 t
ORIGIN      
        1 tttcccctga ccgggtaggt cggaccggtg gtccgctggt aataggctat ggaaaccgtg
       61 aaaatgaagg aaaatatcca gattaaaatc ttttttgttt tcatatgtaa aagctgggat
      121 acaaaaataa aaaaaagcgg gtatccctcg cggggtattt tataaatttg tgcctgctac
      181 caaaaccggc tcaatgaaat caaaagatca gcaacaaccg ccccggaggc aaaaaaatcg
      241 tgttccagga aattcactgt tttccgggcg ggatcgatgg agaattaccg gtgcgctgct
      301 ggtcatttta ctgatctcgc ttctttccta ccttcctgtt tttcataatg aattattgac
      361 ttatgatgac caggcctaca tccagaataa cccgatggtc tattccatgg atctgaaaga
      421 aattttcacc cagaatgtca agagcaatta ccatccggtt actgttctgg tactggcgct
      481 ggaataccgg atgtttggcc tcgatccgac cggatatcat gttttcaacc tgttcctgca
      541 cctgatcaat accctgcttg ttttttatgc tctctacctg ctaacccgca aagccggaat
      601 tgccctggtg gcttcgctgc tgttcggcgt acatcccctg catgttgaat cggttgcctg
      661 ggtggctgag ctgaaggatt tgctttatac gcttttcttc ctgtcctcct atatctttta
      721 cctgaaatac cttgacgaca aaagcaagaa gagttatgca ctggcgctgg gactgttcct
      781 ggtatcgctg ctttcaaagg caatggccgc atcgctcccg cttgtcctga ttttgaccga
      841 ttattttaaa gggagaaaaa tcgacaggaa atccttgatc gaaaagatcc cttttttcct
      901 gtttgcactg gtctttggaa ttgtcgccat tatcgctcag aagtcgacag gggctgccgg
      961 tattgtcgat ctgaatatct tccaacgaat cgttttcgcc tgttatggct tcatttctta
     1021 cttgtttaaa ctggttgtgc cacttaacct gagcgcttac tattcctacc cgatcggcag
     1081 cgggggtacc atccctttgc tgtattatat tttcccggta ctggtggcag gtttggttgt
     1141 gctggtttac ttttcgctac ggtttacaaa gaaggtgatc tttgccgccg gattcttctt
     1201 atttaccgtt ttcttcgtgc tgcaattgct gccggttggt ggtgccgtta tggccgaccg
     1261 ttatgcgtat atcccgtcaa taggcatatt ttacctcgcc ggcgaaggat tttattattt
     1321 gtggaacagg aagcagaagc tagtcccggt catcctgcta agtgcgttca ctatcttttt
     1381 cgccataaca acctattcca ggagcagcgt ctggaagacc gacctgtcgc tctggaccga
     1441 cgttattgag catgatcctt cggtaaaact tgcctatatc aaccggggca atgcgtttaa
     1501 gaatgagaga aaatatgacc gggccgtggc cgatttcagc aaagccatgg agatcgatcc
     1561 ggagtatgcc ttagcttatt ttaaccgcgg atcggcttat gaggatgcaa aaaaagccga
     1621 tctggccctg gccgattata acaaagccct cgaactcgat ccggaactgg cgcaagcctt
     1681 taataaccgc gggaacctgt ttgccgggca gaataaaacc gagctggcat tagccgattt
     1741 tgaacaggca atacgtctgg atcccgggta ttcccaggca tacaataacc gcggcaccct
     1801 ttacgccaat gccaatcaac ccgatcttgc gctggccgat ttcaccaaag ccatcgagct
     1861 gaacccgaat aattccaaag cttataacaa cagggccaat atcgcagtaa accggaaaaa
     1921 gtacgaccag gcgatcgaag atttcagccg ggctatcaaa ctcaacccga ctttttgcca
     1981 gccatattac aaccggggca gtgtgctcag ttatttgaag aagtacgatc aggccatcgc
     2041 tgatttcacc caggctattg ccatcgacgc aaacttcatg caggcttact ttgccagggg
     2101 gcaggcagaa tactactcgg gcaaaaaagc ggcagcctgc cgtgatttca ggtctgcggc
     2161 caacctggga tttaaacccg ctgccgatgc gttacttcag ctttgtaact gatctttttt
     2221 caagggcatc gtagaatacc tggtggattt tcggccggat attcattgcc gaaagcttat
     2281 tgttttctgc attggggtaa acgcggttgg agaggaaaac gaatgtcagt tctgattcag
     2341 gatctaccca gatgtaggtt ccggtgaaac ccgaatggcc gtaacttgac tgcgaggcgc
     2401 ttctacaaca gggcccttct tcggcataca ccggcaaggg cctgtcgaat cccagcccgc
     2461 gccggttatc gttcaatgga aactgcatcc gggtaaattc cccgatgatt tcttccgaaa
     2521 ggtattggtt tccaccgtaa atcccccgat tgagcaacat ctgtgcaatt attccaagat
     2581 cattggcatt ggagaacaat ccggcatgcc cggaaactcc tcctatcatc gccgcgcact
     2641 ggtcatggac atcgccccgg ataagctgac gcctgaaaac agtatcgttc tctgtcggtg
     2701 gaatcctttg aatatcaaat cgttcgcgag gattataccc ggtggtcgac agacccaatg
     2761 gcagataaaa ttctttcccg gcatagctat ctaatcttag tcccgtcagg tttttaatga
     2821 tgggtgtgag aaggataaaa cccaggtcgc tgtatttata ctggctggat ttaagcaaag
     2881 atgatgcttt tatactgtcg aagatgatct tgtcaaaagc ttctgtcaaa aataaatttt
     2941 cagctacccg taccgggaat ttgtctgacc tggtgctccg gtaaaacgca gaatcaggtt
     3001 tgccatcttt cagtgtcgcc ttgtagaacg ggatccacgg tctcagccgt gcctggtgtg
     3061 ccatcatttc gcggactacg atatttcctt tgtcggtgtt tgcaaaatca ggcaggtagg
     3121 tttcaacctt ccggtccaca tcgatcttgc cttcatccgc aaggcgcata acagccaggg
     3181 tggttgcagc gatttttgtc agggaagcga tatcgtaaat gtctgaattt ttaaccggta
     3241 tttcgtttgt atatgtatga tatccgtatg attttgaaaa gaacacttta ccgtcattgg
     3301 cggcgaatac ctggcaaccg ggaaaagctt tctttgaaat ggcgtcgctg atgatcgaat
     3361 cgatgggggc aagcgaggtt ctgtcgagcg caagctcttc ggggatggtg tatttcagcc
     3421 gcatcgatga ggtggttttt cccgtaccgg cgggatagtt gtttcctgca gtaaccggta
     3481 acctgccgtt gatgtctgtt ccgccgaata ttgcctgtgc tgaaagatcc tgcatcagcg
     3541 gatgatcctg gtaggagagg agaatggccg ccggctccgg aagtttttcg atcagcctt
//
LOCUS       DAOQXS010000328         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_26953_length_3593_cov_3.581967, whole genome shotgun sequence.
ACCESSION   DAOQXS010000328 DAOQXS010000000
VERSION     DAOQXS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26953_length_3593_cov_3.581967"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PKM34_09105"
     CDS             <1..111
                     /locus_tag="PKM34_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNQ83788.1"
                     /translation="QLLYFPDENHWVLKPQNGILWQRTFFAWLDNWLKRK"
     gene            167..1129
                     /locus_tag="PKM34_09110"
     CDS             167..1129
                     /locus_tag="PKM34_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNQ83789.1"
                     /translation="MKKLLLVLIMMVPVMIFSQEPAPKKWAVTGISSLNLNQASFSYW
                     TAGGTNSLAFSALGKYSANYKQNKFSWNNNLNLMYGMVKNQGESLKKSEDLIELISVA
                     GLDLNPKWSVTGYMSFRTQFANAWDKDNDSIKVSQLLSPGYLTLSPSVRFKPNDNFYV
                     LISPVTAKFTFVTNQDLADKGSFGVTPAEYDSVGNQWVKVTDGDNMLLYLGPFLEAYY
                     KKTVVKNLTYETRFNVLYTLLNRDNLEGYDADVSWENYLNYSIAKYFNISLLLHLVYL
                     PGQPAIKFDNYEGAVRVKAIPNRHIQIKETIGFGISYIFPAEKK"
     gene            1129..1953
                     /locus_tag="PKM34_09115"
     CDS             1129..1953
                     /locus_tag="PKM34_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ83790.1"
                     /translation="MDEGLNIDELQEKSRLVRTDILRSLAEAGSGHTGGSLGLTDLFV
                     SLYFNILNHKPGVPDWDGRDRLILSIGHVAPVLYATLAHAGYFPKDELLTLRRLGSRL
                     QGHPGREHGLPGLELSAGSLGQGLSVAVGLALAAKADKKSWRVYSVHGDGEMQEGSIW
                     EAAMSAAHYKLDNLIAYIDRNGVQIDGRTGDVMEIEPLADKWRAFGWEVFECDGHDHR
                     RIIETTRNARRIISKPSVIILKTIMGKGVRSIEGDYRWHGKAPSKEELERFLRELA"
     gene            complement(1937..3013)
                     /gene="mnmH"
                     /locus_tag="PKM34_09120"
     CDS             complement(1937..3013)
                     /gene="mnmH"
                     /locus_tag="PKM34_09120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNQ83791.1"
                     /translation="MKKYPVVGKNIFKALGSRPLGRHPLGFRPGGRHPLGFRPGGLID
                     VRSPAEYQQGHIPGAFNLPLFDDRERAAVGTAYTRQGKDAALHLGLELAGPKLTYFVQ
                     KAGEIAQGEEILVHCWRGGMRSEAMAWLLNFAGMKATILPGGYKAYRRSVAELFKNDF
                     RFIVLGGMTGCGKTELIQWLHSIGEQVIDLEKLARHKGSAFGALGQPEQPTQEQFEND
                     LADCLEKLDPGQTIWIEDESRNIGKVIIPGAIYEAVCRSEVVLIERPFVERVNRLLNE
                     YGYFENRELSEIIIKISKRIGGDVAQAALKELESGEVRGAIEIILKYYDKTYAYGLSK
                     RPDDNITRISIEDFKNLAFIRPAP"
     gene            3048..>3593
                     /locus_tag="PKM34_09125"
     CDS             3048..>3593
                     /locus_tag="PKM34_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83792.1"
                     /translation="MPSKMIKTKVLPEKYYHIFNQAINNEFLFREDDDYQFFLLKIKE
                     LIIDYFELYAYCLLPNHFHILLKPLWEVNSIHDSSVNEKLRKLFQLYAQYYNKKYKRK
                     GSLYLKPFRRIEIDTEAYLTYLVFYIHYNPQKHGIIGDYRAYDRSSYKFFICDQETKL
                     QRDVVLKWFGDNPEDFVRFHQE"
BASE COUNT          952 a          820 c          858 g          963 t
ORIGIN      
        1 cagcttctct acttccccga tgaaaaccac tgggtattaa agccacagaa tggaatttta
       61 tggcagagga cattctttgc atggctggat aattggttga aaagaaaata actttatttt
      121 ttaattctta attcttaatt tttaattatt ccataaatat ctaattatga agaagttatt
      181 actagtttta ataatgatgg ttccggtgat gatattcagc caggaaccgg cgcccaagaa
      241 atgggcagtt acagggattt caagccttaa tcttaaccag gcatctttta gttactggac
      301 ggccgggggg acgaattcgc ttgctttttc ggccttagga aagtattcgg ccaattacaa
      361 acaaaacaaa ttttcctgga acaacaatct caacctgatg tacggcatgg tgaagaacca
      421 gggggaaagc cttaagaaga gcgaagacct gatcgaactg atttcggtcg ccggtctgga
      481 tctgaatcca aaatggtcgg ttacagggta tatgtcgttc agaacgcagt ttgccaatgc
      541 atgggacaaa gacaatgatt ccataaaagt ttcccagctc ctttctcccg gttacctgac
      601 attatctcct tcagtacgat tcaaaccaaa tgacaatttt tacgttctta tatcaccggt
      661 aactgccaaa ttcacttttg tgaccaatca ggatctggcc gataaaggct cttttggtgt
      721 gacaccggca gaatatgatt ctgttggaaa ccagtgggtc aaagtgacgg acggcgataa
      781 tatgttgtta tacctcggcc cttttctgga agcatattac aagaagactg tcgtaaagaa
      841 tcttacgtat gagacccgtt tcaatgttct ttacacattg cttaacaggg acaatctcga
      901 aggatacgat gccgatgtaa gctgggaaaa ttacctgaat tattcgatcg caaaatattt
      961 caatatcagc ttgttgctgc acctggtata tttgcccggc cagcctgcga tcaagttcga
     1021 caattacgaa ggggccgtca gggtgaaagc cattcctaac cgtcacatcc agatcaaaga
     1081 gaccatcggc ttcggtattt cgtatatttt tccggccgaa aagaaataat ggatgaagga
     1141 ttgaatatag atgagcttca ggagaagtca cgcctggtcc gtaccgatat tcttcgtagc
     1201 ctggccgaag ccgggagcgg gcatacaggc ggatcgctgg gattgaccga tctgtttgtc
     1261 tcgctgtatt tcaacatttt gaatcataag cctggcgtgc ccgactggga tgggcgcgac
     1321 aggcttatcc tttccattgg gcatgtagcg ccggttttgt atgctactct ggcccatgcc
     1381 ggttattttc ccaaggatga gttattgact ctgcgcaggc ttggctcaag gctgcagggg
     1441 catccggggc gggagcacgg tttgcctggg ctggagcttt cggccggatc gctgggacaa
     1501 ggattgtcgg tggcagtcgg cctggccctg gcagcgaaag cagataaaaa gagctggcgt
     1561 gtttacagtg ttcacgggga tggcgaaatg caggaaggat cgatctggga agcggccatg
     1621 agcgccgcgc attacaagct cgacaacctg atcgcttaca tcgaccggaa tggggttcag
     1681 atcgacggca ggaccggaga cgtcatggag attgagccat tggcggataa atggcgtgct
     1741 ttcggatggg aggtatttga gtgcgatggc cacgatcacc gccgcatcat tgaaactacg
     1801 cgaaatgccc gcaggatcat ttccaaacct tccgtaatca tcctgaaaac gattatgggc
     1861 aagggcgtca ggtccatcga aggcgattac cggtggcatg ggaaggcgcc atcaaaagag
     1921 gaactggagc ggtttctcag ggagctggcc tgataaacgc caaattctta aaatcttcaa
     1981 tgcttattcg ggtaatatta tcatctggcc ttttagacag gccgtatgca tacgttttat
     2041 cgtaatattt caggataatt tcaatcgctc cgcggacttc gcctgattcc agctctttca
     2101 gcgccgcctg tgccacatca ccgcctatcc ttttcgatat tttgataatg atttcggata
     2161 gttcccggtt ttcaaaatag ccatattcat tcagcaacct gttgactcgt tctacgaatg
     2221 gcctctcgat cagcacgact tctgaccggc acacagcttc ataaattgca ccgggaatga
     2281 ttactttccc gatattgcgg ctctcgtcct caatccaaat cgtttgtccg gggtccagtt
     2341 tctcgaggca gtcggccagg tcattctcaa actgttcctg cgtcggttgt tccggctggc
     2401 cgagtgcgcc gaatgccgat cctttatgcc gggccagttt ttccaggtcg atgacctgct
     2461 ctcctattga atggagccat tggatcagct ctgttttccc gcatcctgtc ataccgccca
     2521 ggacaataaa ccgaaaatcg tttttgaaca attcggcaac ggaccgccga taggctttat
     2581 atccacccgg gaggatggtc gctttcatgc cggcaaagtt cagcagccag gccatggctt
     2641 cacttcgcat cccgccgcgc cagcaatgga caaggatttc ttctccctga gcaatttcac
     2701 cagctttttg cacgaagtaa gtcagttttg gtccggctag ttcaaggccg agatggagtg
     2761 ctgcatcttt tccctggcgg gtgtatgcgg tgccgacggc ggcacgctcc ctgtcgtcga
     2821 acaggggaag gttgaaggcg ccggggatat ggccttgctg gtattctgcc ggggagcgga
     2881 cgtcgataag tcccccggga cgaaatccca agggatggcg tcccccggga cgaaacccca
     2941 aaggatggcg tcccaaagga cggcttccca aggctttaaa aatatttttc cccaccaccg
     3001 ggtatttttt catcaaaaac cggattaatt aataaaaata taggattatg ccatcaaaaa
     3061 tgattaaaac aaaagttttg cctgaaaaat attatcatat ttttaaccag gccatcaaca
     3121 acgaattcct cttcagggaa gatgatgatt accaattctt tctgctcaag atcaaagaat
     3181 tgataattga ctacttcgaa ctgtacgctt actgtttatt gcccaatcac tttcatatac
     3241 tactcaaacc attatgggaa gtaaacagta tacatgattc atcagtgaat gaaaagcttc
     3301 gaaagttgtt tcaactttac gcacaatatt acaataaaaa gtacaaaaga aaagggagct
     3361 tatatttaaa acctttcagg agaatagaaa ttgatacgga ggcatatctc acctatttgg
     3421 tcttctatat ccactataat cctcaaaaac acggtattat cggagactat agggcatacg
     3481 atcgtagctc ttataaattc tttatctgcg atcaggagac aaaacttcag cgggatgtgg
     3541 ttttaaaatg gtttggggac aatccggaag atttcgtaag gtttcatcag gaa
//
LOCUS       DAOQXS010000329         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27025_length_3586_cov_3.674880, whole genome shotgun sequence.
ACCESSION   DAOQXS010000329 DAOQXS010000000
VERSION     DAOQXS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27025_length_3586_cov_3.674880"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>452
                     /locus_tag="PKM34_09130"
     CDS             <1..>452
                     /locus_tag="PKM34_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ83793.1"
                     /translation="SFKQKDDVLAGKDGGKWIKYSIDQYIENVNNISYGLLKLGVQPG
                     DKIATISNNRPEWNFVDMAILQIGAVHVPIYPTISESDYKYILKHSEVSFVFISGRDI
                     LRKIEHILPEINAVQDVYTFKAIEGIKHLDELIALGQTNRQSEKLEKL"
     assembly_gap    453..462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <463..1713
                     /locus_tag="PKM34_09135"
     CDS             <463..1713
                     /locus_tag="PKM34_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNQ83794.1"
                     /translation="DDLATLIYTSGTTGFPKGVMLSHRNLLSNVMGVKHIFPVDENCK
                     GLSYLPLCHVYERMDIYTYHYLGLSIYYVENMGTIADNIREIRPEIFTTVPRLLEKVY
                     DKIIAKGSQLKGFKKAIFFWAVSLANRYELEGKGWLYHAQLRLANKLVFVKWREALGG
                     NVRVIVSGGAALQPRLARVYTAAGIPVLEGYGLTETSPVLAVNTLEEGGRMFGTVGKP
                     LKNTEVKIGPDGEILVRGANIMQGYFKEPGMTAEAIDADGWFHTGDVGILEPEGHLRI
                     TGRKKEIFKTSLGKYISPQLLENKFKESAFIDGILILGENQKYAAALVVPDFNHLKSW
                     CEHKNMPYTTDKEMIKNPLVVKRFKKEIDHFNKFFGSTEQIQSFELIDHEWSLETGEL
                     TANLKLKRSYILDKYKEEVSRLFR"
     gene            complement(1717..2715)
                     /gene="holA"
                     /locus_tag="PKM34_09140"
     CDS             complement(1717..2715)
                     /gene="holA"
                     /locus_tag="PKM34_09140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNQ83795.1"
                     /translation="MTYDQITGDLRRKIYRPVYLLHGEEPYFIDAITAMIEESVLTES
                     EREFNQTIVYGRDTDPGKIIDIARRFPMMANYQVVIVKEAQDIDKIENLKPYVEKPVP
                     TTILVLAHKHKKLDQRKGLHKIIEKTGVLFESKKLYDDKIPAWINGQVQAKGYTIKPD
                     ACQLLAEYLGADLGRIINELEKLVISLPAGTAIDSALIERNIGISKDYNFIELQNAIG
                     NRDIHKANRIVNHFTANLKQNPTIALVAILYGFFIKLMIYHQLKDKSPKNAASDMGIH
                     PFFYKDYEKAAKNYSFRRIRPIIAVLREYDLRLKGVNNGSTEEAGLLRELVYKIMH"
     gene            complement(2721..3491)
                     /locus_tag="PKM34_09145"
     CDS             complement(2721..3491)
                     /locus_tag="PKM34_09145"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNQ83796.1"
                     /translation="MKTKEEIVKNWLPRYTGRSLEDFGKYILLTNFDHYVELFANLHN
                     VEITGLDRPMPNATAGGITIINFGMGSANAATIMDLLSAIEPKAMLFIGKCGGLKKKN
                     EIGDLILPIAAIRGEGTSNDYFPPEVPALPAFNLQKAVSTTIREYNHDYWTGTVYTTN
                     RRVWEYDEEFKERLRRMRCMAIDMETATLFIAGFANSIPSGALLLVSDQPMIPEGIKT
                     EETDRQVIRDHVETHLRIGIDSLQLLINDGLTVKHLRF"
BASE COUNT          898 a          836 c          899 g          943 t
ORIGIN      
        1 cttcttttaa gcaaaaagat gatgtactgg caggtaaaga tggtggtaaa tggattaagt
       61 attcgatcga ccagtatatt gagaatgtga acaatatcag ctatggattg ctgaaactcg
      121 gggtgcagcc cggagataaa atcgccacga tctccaacaa ccgcccggaa tggaatttcg
      181 ttgacatggc aattttacag atcggggccg ttcatgttcc gatctatccg acgatcagcg
      241 agtcggatta caaatacatt ctcaaacact ccgaagtcag ttttgtattt atctccggaa
      301 gggatattct tcggaaaatc gagcacattc ttccggaaat taatgctgtt caggatgttt
      361 atacttttaa agccattgag gggatcaaac accttgacga gttgatagcc cttggccaaa
      421 ccaatcgcca atctgaaaaa ctggaaaaac tannnnnnnn nngacgatct ggctaccctg
      481 atatatactt ccggcaccac gggatttccg aaaggagtca tgctttcgca ccggaatctg
      541 ttgagcaatg tcatgggcgt caaacacatc tttccggtgg atgagaactg caaaggcctc
      601 agttatctgc cgctttgcca cgtctatgag cggatggaca tttataccta tcactatctt
      661 gggctttcga tttattacgt tgagaatatg ggcactatcg ccgataacat ccgcgagatc
      721 aggcccgaga tatttacgac cgttccccgg ctgctcgaaa aggtctatga taagatcatt
      781 gctaaaggca gccagctaaa gggatttaag aaggcgatat ttttctgggc tgtgagcctg
      841 gccaaccgtt atgaactcga agggaaaggc tggctctatc atgcacagct ccgtctggcc
      901 aataaattgg tgttcgttaa gtggagagag gcccttggcg ggaatgtccg tgtgatcgtt
      961 tcgggcggcg ccgcccttca gccccgtctt gcccgcgttt acaccgctgc cggcatccct
     1021 gttctcgaag ggtatggcct gacggaaact tcccctgtgc tggccgtgaa cacgctggaa
     1081 gagggtggcc gaatgttcgg aacggttggt aaacctctga aaaacacaga ggtgaaaatt
     1141 ggaccggatg gggagatcct ggtccgtgga gccaatatca tgcaggggta tttcaaagaa
     1201 ccgggtatga ctgctgaagc gatcgatgcc gacggctggt tccataccgg agatgtggga
     1261 atcctcgaac ccgaaggaca cctgcggatc accggaagga aaaaagaaat ctttaaaaca
     1321 tcgctgggga aatatatcag cccccagttg ctcgaaaata aattcaaaga atcagccttt
     1381 atcgatggca tccttatcct gggcgaaaat cagaagtacg cagcagcgct ggtcgttccc
     1441 gattttaacc acctgaaatc gtggtgcgaa cataagaata tgccttatac aactgataaa
     1501 gagatgatta aaaatccgct ggtggttaaa cggttcaaga aagagatcga tcatttcaac
     1561 aaattctttg gcagcacaga gcagattcaa agcttcgaac tgatcgacca tgaatggagc
     1621 cttgaaacag gggagctgac ggcaaacctc aagcttaagc ggtcatatat cctcgataaa
     1681 tataaggaag aagtcagcag gcttttccgc tagatatcag tgcatgatct tgtaaaccaa
     1741 ttccctgagc agccctgcct cctccgtaga accgttgttc acccccttaa gccgcaggtc
     1801 atattcgcgt aacacagcga tgatgggtct tatccggcga aagctgtaat tctttgccgc
     1861 tttttcataa tctttataaa agaaggggtg gatccccatg tcggatgcag cgttttttgg
     1921 ggatttatct ttcaattgat ggtaaatcat cagttttatg aagaaaccat ataaaatggc
     1981 aacgagcgcg atggtcggat tctgcttcag gttggccgta aaatggttca caatgcggtt
     2041 agctttgtga atatcgcggt taccgatagc attctgaagc tcgatgaaat tgtaatcttt
     2101 gctgatgccg atgttcctct ctatcagggc tgaatcgatg gcggtgccgg cgggaaggct
     2161 gatgaccagc ttttccagct cattgatgat ccggcccagg tcggcgccca ggtattcggc
     2221 cagcaactgg caggcatccg gtttgatcgt gtagcctttg gcctggacct gcccattgat
     2281 ccacgccgga atcttatcat cgtataattt cttcgactcg aataatactc ccgttttttc
     2341 aatgattttg tgcaagcctt tccgctggtc gagtttcttg tgtttatgcg caagtaccag
     2401 gatggtggtc ggaaccggtt tctcgacata gggcttcagg ttttcgattt tatcgatatc
     2461 ctgcgcttct tttacgatca ctacctggta attggccatc ataggaaagc gccgggctat
     2521 gtcgatgatc ttacccggat cggtatcacg tccatataca atggtctggt tgaactctct
     2581 ttcggattcg gtcagaacgc tctcctcgat catcgcggtg atggcatcga taaaataggg
     2641 ctcttctcca tgaagaaggt aaacgggacg gtagatcttt cgccggaggt cacctgtgat
     2701 ctggtcgtaa gtcatagtga tcagaagcgt aagtgcttga cagtgagtcc gtcgttaatc
     2761 aacaactgca gtgaatcgat accgatccga aggtgggttt ccacatggtc acggatgacc
     2821 tgcctgtcgg tttcctcggt tttaatccct tcagggatca tgggctggtc gctgaccagc
     2881 agcagggcgc ccgacggaat gctgttggca aagccggcga tgaagagggt agcggtctcc
     2941 atatcgatcg ccatacatct cattctgcgg agccgctcct tgaactcctc gtcatattcc
     3001 catacccgcc ggttagtggt gtaaaccgtg cctgtccaat agtcgtggtt atattcgcgg
     3061 atggtggtcg aaacggcttt ctgcaggttg aatgcgggca atgccggcac ctcgggagga
     3121 aaataatcgt tggaggtccc ttcgccgcga atggccgcta taggaaggat cagatcgcct
     3181 atctcgttct ttttcttcaa tccgccgcat ttgccaataa acagcattgc tttgggttcg
     3241 atggccgaca acaaatccat gatcgttgcg gcattcgcgc tccccatacc aaaattgatg
     3301 atcgttattc cgccggctgt cgcattgggc atggggcggt caagtcccgt gatctcaacg
     3361 ttgtggaggt tagcaaacaa ctcaacataa tgatcgaagt tggttagcag gatgtacttt
     3421 ccgaaatctt ccaacgaacg gcccgtataa cgcggcagcc agttctttac aatctcctct
     3481 ttggtcttca tccgttccgt ttttgcaaag gtaggatttt tgttaatttt gagatggaat
     3541 tccgtgctga agaactcccc gaaaataatg ataaaactga atttcc
//
LOCUS       DAOQXS010000330         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27143_length_3575_cov_4.299148, whole genome shotgun sequence.
ACCESSION   DAOQXS010000330 DAOQXS010000000
VERSION     DAOQXS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27143_length_3575_cov_4.299148"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1281
                     /locus_tag="PKM34_09150"
     CDS             358..1281
                     /locus_tag="PKM34_09150"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83797.1"
                     /translation="MKINPKYISFVALVFALSLSLTHAQPVVGIYPPQVHQQICQEST
                     YTRYLNVFNTGDSLLIFNAAFTPGPYSWVTAEPLQGEVAPGDTIQIAFTFNSAGLAID
                     NYYSYLLLSCNDPKNPEQSILGMLHVQDITIFLEPESDSICAGCSTKLTTIAFGCSEE
                     YQFHWTSDPPGFTSSEKSPVVAPLVNTTYTVKVTDGGGSAEKSVFIRVYGSSDVEENT
                     SLSKFSVFPNPTDGPFILSLYTEKEGSAMVHIYSVQGKEVYSHSIDLHRGDNEYFIDH
                     TGPGPGMYFIIVDKVTATADFHRFCGRIMVK"
     gene            1507..2682
                     /locus_tag="PKM34_09155"
     CDS             1507..2682
                     /locus_tag="PKM34_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNQ83798.1"
                     /translation="MMITYEEALEIVLRNTYLFDKETVRIQEAIGRALADDVFSDINM
                     PPFNRSAVDGFACRKADLGQPLEVIETVRAGLFPQKKIGTGQCTRIMTGAPVPEGADC
                     VMMVEETEETADRRISFRGSQTKTNISLFAEDVREGDCVLKAGTMIAPHHIAIMSAVG
                     CVRPVVSKKPRVAVIATGDEIVEPHLKPGISQIRNSNGYQLTAQALRSGAEANYMGIA
                     PDTEEDTFRILDNALDQADVILLTGGVSMGTFDFVPKILEKAGIEILFRTIALQPGKP
                     TVFGVKGDKRIFGLPGNPVSSFTIFEILVKPMLWKMMGITEPVRNMILTFGGHYQRKR
                     GDRMQWLPVKIKAGKVYPLEYHGSAHIFALADADAIAAIPLGITELNEGDLIDVRPV"
     gene            2666..3466
                     /locus_tag="PKM34_09160"
     CDS             2666..3466
                     /locus_tag="PKM34_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ83799.1"
                     /translation="MFDRFDRQINYLRISVTDRCNLRCTYCMPADGITLLRHEDILSY
                     DEIVEVAQIAAEMGVSKIRLTGGEPLVRKGITNLVKMLASIPGIVDLSMTTNGILLER
                     FAQELKDAGLQRVNISLDTLDAERFRKITRGGDIEEVKKGIMKAREVGLTPVKINCVT
                     GYEMDPEDAARVKEFSEANGFQVRFIRQMDLASGEFYVVDGGDGGNCSRCNRLRLTSN
                     GIVKPCLFNAIGYNVRELGAREAILQAVSNKPRCGTANSEEEFSWIGG"
BASE COUNT          944 a          830 c          868 g          933 t
ORIGIN      
        1 gtaattacag ttgctatagc cttgttgttg gtaatggggc aataattaca attcagtcac
       61 cgtatgtgct taatgtattg aatccatgat agatacttga tccccattta aaatcttcag
      121 ccccgccact ctattggcgg ggcttttttt gcgttttttt catgttccgt tttgcatttt
      181 tcattttgcg ttttgcattt catttttcat ttagcatttc atttttcatg ctaagtttca
      241 tttatcgtct atttaaaatg ggtcatgcaa aatgccatga aaaatgaaaa aagaaaaatg
      301 caaaaaggaa catatacctg ttctgatata tttttgtgcc tgaataatat ccgataaatg
      361 aaaatcaatc cgaaatatat ttcctttgtt gccctggtat ttgccctttc ccttagcctg
      421 acccatgccc agcctgttgt gggaatctat cctccccagg tccatcagca gatatgccag
      481 gaaagtacct atacgcgtta cctgaacgta ttcaataccg gcgattcgct ccttatcttt
      541 aatgctgcat tcacgcccgg gccttattcg tgggtcacgg cagaaccgct gcaaggcgaa
      601 gtggctccgg gcgacacgat acagatcgcc tttactttca attctgccgg actggccatt
      661 gataactact actcttacct cctgctttcc tgcaacgacc ccaaaaatcc ggaacaaagc
      721 atcctgggta tgctgcatgt ccaggatatc accattttcc tggagcccga gagcgacagt
      781 atctgtgccg gctgtagtac taaactgact acaatcgctt tcgggtgctc cgaagaatac
      841 cagttccact ggacctccga tccacccggt ttcacgtcat ctgagaaaag cccggtcgtt
      901 gccccactgg tgaatacgac ctatacggtc aaggttaccg acggaggcgg atcagccgag
      961 aaaagtgttt tcatccgggt atatggatct tctgatgttg aggaaaatac ttctttgtct
     1021 aagttttcag tttttccgaa tccgactgac ggccctttca tcctttcgct gtacactgaa
     1081 aaagagggat cagcgatggt gcatatttat tctgttcaag gaaaagaagt gtattcacat
     1141 tcaatcgacc ttcatagagg agataatgaa tatttcattg atcacacagg gcccggtccg
     1201 ggaatgtatt ttattattgt cgataaagta acggccacag ctgatttcca ccggttttgc
     1261 ggaaggataa tggttaaatg aagtattact ctgtgctact ctgtgtaaat ctgtatcact
     1321 ccgtggtaaa attagttttt gttacaccac agagtacaca gagaacacag agttatcata
     1381 aagagagtta actgcttatg atgaggcagg ctaaaaatcc ccgggaaccg gcttccgttt
     1441 cagtcgtcaa acttagccgt aagtttattt ggcttaatat tcctactttt acaggctcat
     1501 ttttttatga tgatcacata cgaagaggca ctggagatcg ttttaagaaa tacttatcta
     1561 tttgataaag aaacagttcg aattcaagag gcgattggaa gagcactggc agatgatgta
     1621 ttctcagata tcaatatgcc cccgttcaac cggtcggccg tggatggatt tgcctgtcgc
     1681 aaagcagacc tcggacaacc gctggaggtg atcgaaacgg tccgcgccgg cttgtttcca
     1741 cagaaaaaga ttggcacggg gcagtgtaca cgcattatga ccggagcgcc tgtccctgaa
     1801 ggggccgatt gcgtaatgat ggttgaagag acggaggaga cagccgatag acggatctcg
     1861 ttccggggat cacaaaccaa aacaaatatc tccctgtttg cagaagatgt aagagaagga
     1921 gattgtgttt taaaagccgg cacgatgatc gctccgcatc atatcgccat tatgtctgct
     1981 gtgggctgtg ttcggcccgt agtttccaaa aaaccgcgag ttgcagtcat cgcaaccggc
     2041 gacgaaatcg tcgaaccgca ccttaagccg ggcatctccc agatccgcaa cagcaatggt
     2101 tatcagctca ctgcccaggc cctaagatct ggcgctgaag caaactatat gggtattgcc
     2161 cccgatacgg aagaagatac attcaggata ctcgataacg ccctggatca ggccgatgtg
     2221 atcctcctta ccggcggcgt ttcgatgggc acatttgatt tcgtccccaa aattctcgaa
     2281 aaagcaggca ttgaaatcct tttcaggacc attgccttgc aacccggtaa accgaccgtt
     2341 ttcggcgtaa aaggagataa gcgcattttc ggattgcccg gaaatccggt ctcttcgttt
     2401 actatttttg aaatcctggt gaagccgatg ttatggaaaa tgatgggaat aacagaaccc
     2461 gtcagaaata tgatactgac ttttggaggg cattatcaac gaaagcgggg cgaccggatg
     2521 caatggctcc cggtaaagat taaagctgga aaggtttatc cgctggaata tcacggatcg
     2581 gcacacatat ttgcactggc cgacgccgat gccatcgccg ccatacctct cggaatcact
     2641 gaactcaatg aaggagatct gatcgatgtt cgaccggttt gaccgccaga tcaattacct
     2701 gcgcatctcg gtcactgacc ggtgcaacct gcgctgcact tattgcatgc ctgccgatgg
     2761 gatcacgttg ctgcgacacg aagatatcct gtcgtacgat gaaattgttg aagtagcgca
     2821 aatagctgcg gaaatgggtg tcagcaagat caggctgacc ggcggagaac cactggtgcg
     2881 caaaggaata accaacctgg taaagatgct ggcctcgatc cccgggatcg tcgatctgtc
     2941 gatgaccacc aatggcatcc tacttgaaag atttgcgcaa gagctgaaag atgccggtct
     3001 tcagcgggtg aatatcagcc tcgatacgct ggatgcggaa aggttcagaa aaatcacccg
     3061 gggaggcgat attgaagagg tgaagaaggg aattatgaaa gccagggagg tcgggttaac
     3121 cccggtcaag atcaactgcg ttacaggtta tgagatggat ccggaggatg cagcccgggt
     3181 gaaagaattc agcgaagcaa acggattcca ggtccggttc atccgtcaga tggacctggc
     3241 ttcgggtgaa ttttatgtgg tagacggagg cgacggagga aactgcagcc gctgcaaccg
     3301 tctccggctg acctccaacg ggatcgtcaa gccgtgcctg ttcaacgcca tcggctacaa
     3361 tgtcagggaa cttggggcaa gggaagctat tttgcaggcc gtcagcaaca aaccccgctg
     3421 cgggacagcc aattctgaag aggagttttc gtggatcgga ggatgagata agactatagt
     3481 cattagctca cttcgttcgc gtcattggct tgctgcgcaa gcgtcattgg aaatatcgaa
     3541 ttatctttga actttgaact ttgaactttg aactt
//
LOCUS       DAOQXS010000331         3567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27234_length_3567_cov_3.588554, whole genome shotgun sequence.
ACCESSION   DAOQXS010000331 DAOQXS010000000
VERSION     DAOQXS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27234_length_3567_cov_3.588554"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PKM34_09165"
     CDS             <1..176
                     /locus_tag="PKM34_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HNQ83800.1"
                     /translation="AASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVMIALVNV
                     AFWFKRTYFKPVE"
     gene            260..679
                     /locus_tag="PKM34_09170"
     CDS             260..679
                     /locus_tag="PKM34_09170"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNQ83801.1"
                     /translation="MGKKILVICTGNSCRSQMAEGFIRSFDKTLKVHSAGTHPADKVN
                     PNAVKVMKEIGIDISDHVPELVDKYVNEEWDYVITVCGGAKEVCPAFTGTVKHRLHIG
                     FDDPADAVGSEEEVMAVYRRVRDEIKVSFIEFYLKIR"
     gene            698..1522
                     /locus_tag="PKM34_09175"
     CDS             698..1522
                     /locus_tag="PKM34_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNQ83802.1"
                     /translation="MEDVKKIVREKYGEIARENAEPEKKTSCGCGCGCDDTYTIFSEN
                     YNGKDGYDPDADLGLGCGIPTDFVQIHKGDTVIDLGSGAGNDCFVARALVGEEGKVIG
                     IDMTKEMIELARKNADKLGYKNVQFRYGDIENMPVTSKKADVVISNCVLNLVPDKEMA
                     VSEICRVLKVGGHFCISDVIIKGELPEKIRNAAEMYAGCVSGALEREKYMELIARMGF
                     INISIHKEKEILIPDNILSGYLSKEEIEAYRKSGTGIFSITISGEKPAKGCVCCGT"
     gene            1798..3093
                     /locus_tag="PKM34_09180"
     CDS             1798..3093
                     /locus_tag="PKM34_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ83803.1"
                     /translation="MPINRRSFVRQFGATLGAASLGTALLPGFALGKEYEDPDYMNFP
                     PAGTPEDDFWAWVQQNYTVSPNIINLNNGGVSPSPRPVQDTFERYNRYCNEAPSYYMW
                     RILDQGREALRADLAALAGCSPGEIAINRNTTEAMDIVMYGLRLQKGDEVVLTKQDYP
                     NVINLWKMLEQRDGIVLKWVNLELPMEDDEALAQKYIDAFTGRTKVVNVTHIINWIGQ
                     IVPVKRIAAEAHKRGIDVLVDGAHTFGLLDFRIPDLDCDYFATSLHKWLGAPFGSGLL
                     YVKKEKISKIFPIYPDDKYMGDEIQKFERMGTRNFASEMAIGSALDFHLAIGSKRKEE
                     RLRYLKNYWMEQVKDIPGVNLHTSFKPEYSCVIGLFSIDGYTPGEIDTYLFDKHKIHT
                     VGIVWENISGVRVTPNVYTMERDLDKLVQGITQLAKQKK"
     gene            3116..3559
                     /locus_tag="PKM34_09185"
     CDS             3116..3559
                     /locus_tag="PKM34_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNQ83804.1"
                     /translation="MKKAGFSLLFIFLLFTGISQTIEKKIIQAPDAPKAIGPYSQAVM
                     AGNTLYVSGNIALNPENGQMDTLNLEAETRRVMSNLGAVLKEAGMSYENIVKATIYMT
                     DIRNYKEINRIYGEYFKEDPPAREAIQVVALPAGAHVEISCIAVK"
BASE COUNT         1016 a          746 c          946 g          859 t
ORIGIN      
        1 ccgcagcaag caacaatttt gaacttgcta tcgcggtggc tattgctgta tttgggatta
       61 attccggaga ggctttcgct gctgtgatcg gcccgcttgt tgaagtgccg gtaatgattg
      121 cgctggtaaa tgtggcgttc tggtttaaaa ggacctattt taaaccggtt gagtgaaatc
      181 atcttaaacg tatattcaaa ccaaaaccgc aaggagacgt taacattgag tacttttgcg
      241 tagaagttag catagtttta tgggtaagaa aattttagta atttgtacgg gaaacagttg
      301 ccgcagccag atggcggaag gttttataag gtcgttcgat aaaacgctga aggttcattc
      361 agcaggaacg catccggccg ataaagtcaa tcccaacgcg gtgaaagtga tgaaagagat
      421 cggcatcgat atcagcgatc atgttccgga actggttgat aaatatgtta atgaggaatg
      481 ggattatgtg atcaccgttt gcggcggtgc aaaagaagtc tgcccggcat tcaccggcac
      541 agtgaaacat cgactgcata tcgggtttga tgatccggca gatgcggtag gcagcgaaga
      601 ggaggtgatg gctgtgtacc ggagggtgcg ggatgagata aaagttagct ttattgagtt
      661 ttatttgaaa attagataat ttggaaattg ggtaattatg gaagatgtaa aaaagattgt
      721 ccgtgagaaa tacggcgaga tagccaggga aaatgccgaa cctgaaaaga aaacctcttg
      781 cggttgcggt tgcggatgtg atgatacgta taccattttc agcgaaaatt ataacggaaa
      841 ggatggctat gatccggatg ccgacctggg cctgggttgt ggcattccga ccgattttgt
      901 tcagattcat aaaggcgata cggtaatcga cctgggctcc ggcgccggca acgactgttt
      961 tgtcgcccgt gcactggttg gagaagaggg caaggtcatc ggcattgata tgacgaaaga
     1021 aatgatagaa ctggcaagga aaaatgcaga taaactgggc tataaaaatg tccagttccg
     1081 ctacggcgat atagagaata tgccggtgac atccaaaaaa gccgacgtgg tgatcagcaa
     1141 ctgtgtactt aacctggtcc cggataagga aatggctgtg tcggaaatct gccgggtgct
     1201 taaggtcggg gggcatttct gcatttccga cgtgatcata aaaggagaat tgcctgagaa
     1261 aatccggaat gcagctgaga tgtatgccgg atgtgtttcc ggtgcacttg aacgggagaa
     1321 atatatggaa ctcattgcca ggatgggatt tataaacatt tctattcata aagagaaaga
     1381 aatattgatt ccggataaca tattgtcggg ttatctttca aaggaagaaa tcgaggctta
     1441 caggaaatca ggtaccggaa tcttcagtat cactatctcg ggtgagaaac cggccaaagg
     1501 atgtgtttgc tgcgggacat aaagtcaaaa aaagccttta aaattaaaat caataattta
     1561 ctcgcggtag gtaagaggga agatcaatga tttagggcct ttccggaggg aagtcgaatt
     1621 agaatgagat ggcatcccaa atccaataaa ataactttgt aaccgtcaga aagacagatt
     1681 tataaccgag aatatcagtt attctgagat gccatctcat gagattcttt ttttggctga
     1741 cttgttgaat cattcgttct ttttatttcc taaatttgaa ccttaatact aactttaatg
     1801 ccgatcaacc gccgcagttt tgtcaggcag ttcggcgcca cgctcggggc ggcttccctg
     1861 ggcactgcgc tcctgccggg ctttgccctg ggcaaagaat atgaagatcc cgactatatg
     1921 aattttcctc cggccggtac gccggaagat gacttctggg cctgggtaca gcagaattat
     1981 acggtctcgc ccaatatcat caacctgaac aatggtggcg tgagcccttc gcccaggccg
     2041 gtgcaggata cgtttgagcg ctataaccgc tactgcaatg aggcgccgtc gtactacatg
     2101 tggcgcatcc tcgaccaggg aagggaagcg cttcgagccg acctcgccgc cctggccggc
     2161 tgctccccgg gcgaaattgc catcaaccgg aataccaccg aggccatgga cattgttatg
     2221 tacggcctcc ggttgcagaa aggcgatgaa gtagtgctta caaagcagga ctatcctaac
     2281 gtcatcaacc tctggaagat gctggaacag cgcgacggga tcgtgttgaa atgggtaaac
     2341 ctggagctgc ccatggaaga tgacgaagcc ctggctcaga aatatatcga cgcattcacc
     2401 ggcaggacca aagtggtgaa cgtcacccat atcatcaact ggatcgggca gatcgtcccg
     2461 gtgaaaagga tagcggcaga agcccataaa agagggatcg acgtcctggt tgatggtgcg
     2521 cataccttcg gcctcctcga tttcaggatt cctgacctgg attgcgacta ctttgccacg
     2581 agcctgcata aatggctcgg agcaccgttc ggatcaggtc tgctgtatgt aaaaaaggaa
     2641 aagatatcaa agatttttcc gatctatccc gacgataaat atatgggcga cgaaatccag
     2701 aaattcgagc ggatgggaac gcggaatttt gccagcgaga tggctatcgg cagcgcgctc
     2761 gatttccacc tggccatcgg ctcaaaaaga aaggaggaaa ggctgcgcta tctgaagaat
     2821 tactggatgg aacaggtgaa ggacataccg ggcgtaaatc tccatacatc gttcaaaccg
     2881 gaatattcct gcgtcatcgg gctgttttcc atcgacgggt ataccccggg agaaattgac
     2941 acatacctat tcgacaagca caagatccac accgtcggga ttgtctggga aaacatttcc
     3001 ggagttcgtg ttactccgaa cgtttatacc atggaacgcg atctcgacaa gctggtacag
     3061 gggattaccc aactggcaaa gcagaaaaaa taacatctga taatatattt taaccatgaa
     3121 aaaggcaggt ttcagtttgc ttttcatctt cctgttattc acaggaattt ctcaaaccat
     3181 cgaaaagaag atcattcagg cgccggatgc gccaaaagca attggaccct acagccaggc
     3241 tgtcatggcc gggaatacgc tctatgtttc gggcaatatc gctttaaatc cggaaaacgg
     3301 acagatggat acattgaacc tggaggcaga aacaagaagg gtgatgtcga acctgggcgc
     3361 tgtattgaaa gaagcgggca tgtcgtatga gaatattgtc aaagccacga tctatatgac
     3421 cgatatcagg aattacaaag agatcaaccg gatttacggc gaatatttca aagaagatcc
     3481 tcctgcccgt gaagccatac aggtggtggc actgccggcc ggagcacacg tggagatttc
     3541 gtgtattgcg gttaaatgag tttcgtg
//
LOCUS       DAOQXS010000332         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27301_length_3562_cov_3.691474, whole genome shotgun sequence.
ACCESSION   DAOQXS010000332 DAOQXS010000000
VERSION     DAOQXS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27301_length_3562_cov_3.691474"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1387
                     /locus_tag="PKM34_09190"
     CDS             377..1387
                     /locus_tag="PKM34_09190"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNQ83805.1"
                     /translation="MDLTTTYLGLKLKNPIIVGASNLVTDLNFLKKLEDAGASAIVYK
                     SLFEEQIQLENLENYHAEEDYGARHAEMSSMLPLIEDAGPAEFLYNLTQARKAVNIPL
                     IASLNAVYDDTWYEYARKIEQTGVDALELNFYAVPREFDIDDNSILQEQLDTLEGVKK
                     SVKIPVSVKLSPFYTNPLYCISELDKKGADGFVLFNRLFQPDIDLDKEGLHYPYMLSN
                     EEDNRLPLRFAGLLYSKIRGSICSNTGILNGKDVLKMILAGADAVQIVSTIYKHGPKQ
                     IGKILEEMEEWMKSKKYNSLADFKGKLAKVNIKDPYAYQRAQYVDILMKSNEIFKKYP
                     IR"
     gene            complement(1577..3556)
                     /locus_tag="PKM34_09195"
                     /pseudo
     CDS             complement(1577..3556)
                     /locus_tag="PKM34_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
BASE COUNT         1004 a          845 c          723 g          990 t
ORIGIN      
        1 agattgctgc cagttcgctg aacatgtatg caacatgagc cgccgcgtaa ttcccatcgc
       61 aggtaataaa cttcttattt ttcataatat attgtattta aacactttaa aacaataaaa
      121 tcctaatttc taaaatccag tgcgaaatta ctaaattata tccacgggga tggattggcg
      181 cttcaaaatc gcttttcaat agcctaattt ataaccattt tagataaatt taaaggctga
      241 aatatttccg aaatttcctt tttataccgc attctcattt ctatttcttt tataaaatct
      301 tataataatc cttgtatata taggtagctt tatctacttt tacgaccgtt ttcaaactta
      361 aaatacaggt aataaaatgg atttaacgac gacatatctt ggcctcaaac tgaagaatcc
      421 cataatcgtg ggtgccagta acctggtcac cgatctgaat ttcctcaaaa agctggaaga
      481 tgccggcgca tcggccattg tttacaaatc attgttcgaa gagcaaattc agctggagaa
      541 ccttgaaaat tatcatgctg aagaagacta cggtgcacgt catgcagaga tgtcatctat
      601 gctgcccctc atcgaagatg ccggcccggc ggaattcctc tacaacctga cccaggcgcg
      661 caaggctgtg aatatccccc tgatcgccag tctgaacgcc gtatatgatg atacatggta
      721 tgaatatgcc cggaagattg agcaaaccgg cgttgacgca cttgagctga acttctacgc
      781 agtacccaga gaatttgata tcgatgataa ttctattctt caggagcagc tcgatacact
      841 cgaaggggtt aaaaaatcgg ttaaaatccc cgtcagtgta aaactcagtc cgttctatac
      901 caatccgctt tactgtatca gcgaactgga taaaaaaggg gccgacggtt ttgtcctgtt
      961 taaccgcctg ttccagccgg atatcgacct ggataaagaa gggcttcact atccgtatat
     1021 gctctccaac gaggaagaca accgccttcc gctgagattt gccggattgc tttattcgaa
     1081 gattcgcgga agtatttgca gcaataccgg tatactgaac ggtaaggacg ttttaaagat
     1141 gatccttgcc ggagccgatg ccgtgcagat tgtcagcaca atttacaaac atggccccaa
     1201 acagatcgga aagatactgg aagagatgga agagtggatg aaatccaaaa aatataactc
     1261 actggccgat ttcaaaggta agctggccaa agtgaatatt aaggatccct acgcatatca
     1321 aagagctcaa tacgtggata tcctgatgaa atcaaacgaa atatttaaaa aatatccgat
     1381 ccgttaaatc catgccggat aaaaagaggg tgtctcaaaa gtacaaatat tcgcttttaa
     1441 ttggtagtca gacgactgaa gccgtcggtt cccaccttcg caaaacctta ggaaccaacg
     1501 gctttagtcg ttgaagtacg aactaataac aggccattat tgtgaaatat caggctattg
     1561 agacagcctc ttttttttat tctatcccga tctcatcgat aaaaagccag gccttctctc
     1621 ccgatccgac atgccattcc gggcaaacgc ccctgttctt cgcattaact tttatatacc
     1681 ttgccatcat acctacatcc ttaaggtcgt attctacaac ctgggctatc tctgatttat
     1741 cctcgaaaac cgatttccgg gtcccagctt ttttataatg gatattatct tctgaaagag
     1801 aaaattccac ctccccgggt aaaaatatcc acgaccgctg atcgtgcaag aacctggctg
     1861 aaagtgaatg aataaaagtc attctgccca ggtcgattac agcttcgaaa tcaacgccat
     1921 gatatccctg ccatgaaccg gttctaaagt tcgggcctcc atactgcctg tcaatcaggg
     1981 caatatctcc tcctcccgtg tactggctat tgtagagatg agttatagaa acgtgccatt
     2041 catggtcaat cttgtgaaaa gcagcattgg taattgaact tggcacctgg ccatccgttt
     2101 ctgcataggc agacaaattc aacgatacat cagaatgtat tggcgcggtg tacgttacaa
     2161 agttattttt atcggcgatt tcgcctgtat tgtaaaacaa ccgggcatcc ggatcgacat
     2221 gccggagttc tacgactata ctatcctgga aagaagccga ggtgctctta taataaggta
     2281 taggagtcag gatggggtcc agtatcgagg tctcaggaat gtcgtttccc ccttttcccc
     2341 attcaaggcc aggttccggc cccaatgtaa acgcgataac tccaccgttc atgatatcag
     2401 aattgtcgat atatgaacgg gtgtatgact tcccgtttaa tgacgcggat tgaatgtaga
     2461 tgtttctttc atcattgccc ctggcggata tttgaaaagt tttacctcct tcgagatgca
     2521 aaatgacctc atcaaacaag gggcttccga taacataata tccggatccc ggggttaccg
     2581 ggtaaaatcc catggcactg aggacaaacc atgcagacat ctggccgcag tcttcattcc
     2641 cgcataatcc atcgggaccg gtgccataaa gttcatccat gatttgcctg accagccgct
     2701 gggttttatc cgggctcccc gtataattat ataagtaggc catatggtga ctgggttcat
     2761 ttccatgggc atactgtccg attagtccgg ttatatcggc ttgttgccgt ccactaaggt
     2821 tctccggtgc tcaaaacatt tcatccagcc tattttcaaa ttgttctttg cctcccatca
     2881 gcgccattaa accggaaata tcctggggcg cataaaatgt gtattgccat gtgttggctt
     2941 cagtcagcat aaagttgact tcagccggat caaaaggttc ggcaaacatt ccatttcgtt
     3001 tccctctcaa aaacccggtt tcgggatcaa acagattttt ataatattga gctctcatta
     3061 tgaagttcct gtaatcttct tccctgccca tctgcgccgc cacttttgcg atgcaccagt
     3121 catcataggc atattccagt gtttttgata ctgcttctcc ttctgcttcg gccgggataa
     3181 aaccaaattt ccggtaacta tccaatccgt aatggtcctg tccggcgctc cccttcatcg
     3241 cagcaaatgc tttctcgtga tcaaatcctc tataaccttt cagataagca tcggcaataa
     3301 cagggactgc atggtagccg atcatgcaga atgtttcatt gccagccagt tcccaaaccg
     3361 gaagcaagcc ccccttttca taaatatcga tgaaagtttt gataaaatca ttggttcttt
     3421 ccggttgaac gatggtcatt aatgggtgta atgcccggta agtatcccag agggaaaaaa
     3481 cagtgtaaac atcaaacccg ccatggtgaa tggctccgtc gtgaccacgg taacggccat
     3541 ccgtatcaga aaacaggttc gg
//
LOCUS       DAOQXS010000333         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27331_length_3560_cov_3.160913, whole genome shotgun sequence.
ACCESSION   DAOQXS010000333 DAOQXS010000000
VERSION     DAOQXS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27331_length_3560_cov_3.160913"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKM34_09200"
     CDS             complement(<1..861)
                     /locus_tag="PKM34_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ83806.1"
                     /translation="MKHPDIEIIKSIIKDSHSVVVTSHHNPDGDAIGSVIAMVKLLEK
                     RVPQVTGIVPNDFPDFLKWMPGADRIVIYNQLEPKAKELIYNADLIFCLDYNALHRTD
                     AMAPALKSARAVKVLIDHHLDPLSEDFDYIISRIDISSTSELLFNFIEQCGWSDLIGQ
                     EVATGLFVGIMTDTGSFSYSCNYPETFQISALLIKTGIDPEQIHRNVYDTYSESRLRL
                     LGYCLGEKMTILPQYKTAYIALTKAELDRFQYKTGDTEGVVNYALSIDEIRFAVLLTE
                     RTNRIRLSFRS"
     gene            940..1359
                     /gene="ndk"
                     /locus_tag="PKM34_09205"
     CDS             940..1359
                     /gene="ndk"
                     /locus_tag="PKM34_09205"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ83807.1"
                     /translation="MNGNITLTMIKPEAVRNGHTGHILAKITEGGFGIVAMKYLKLTR
                     EQAEKFYEVHRERPFYKDLVEFMTSGPIVAAILKKDNAVEEYRSYIGSTNPKDAAPGT
                     VRALYGTNIQENAVHGSDSDENAAREASFFFSELERY"
     gene            complement(1353..1754)
                     /locus_tag="PKM34_09210"
     CDS             complement(1353..1754)
                     /locus_tag="PKM34_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ83808.1"
                     /translation="MIDRFRQLMADQGLSASEFADRIGVQRSSISHILSGRNKPSIDF
                     LEKIKTAFPEADLNWLVSGKKSATSGLSTSPAVSKTVPVSLPPTLFEAEENNEIQKKP
                     VHKDDEPVEQIIMIFRNGTFRVLKPESGKDQ"
     gene            complement(2165..>3560)
                     /locus_tag="PKM34_09215"
     CDS             complement(2165..>3560)
                     /locus_tag="PKM34_09215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNQ83809.1"
                     /translation="VTVFTDNPDVLEAIDALPYVAGITGYPQEKYQPGRIQNDKEKLF
                     FYHEMYNQGPGNSFKNKAQTGAFDYGMGFNQIQMLNGDVFHEMGYRGQGKVIAVLDAG
                     YFNANSVDAFDSLWMNGQILGTKDFVRGGDIQFDEHQHGTMVLSCMGANSPGQLIGTA
                     PMADYWLLRSEDGGSEYLIEEYNWVSAAEFADSVGADIINSSLGYSQFNDPTQDHTYE
                     DMDGNTTPATKGADMAAKKGILVVNSLGNEGNSSWYYLSAPSDGDSVMGIGAVDANGF
                     YVGFSSHGPSYDGRVKPDVATQGSMVYVVEPMGGGFTFGGGTSFSSPILAGMAACLWQ
                     ANPAMTNMQIADAIRKSASQYDNPDDMLGYGIPDFVLANNILTVINGPDKQHVSLNLY
                     PNPVRTSFTVSLDQVNANNADIIITDITGREIYTASLMGNEYTVDAKGLEAGVYFVSV
                     SANGRTFTGKFIKR"
BASE COUNT          974 a          804 c          763 g         1019 t
ORIGIN      
        1 cgaccggaat gatagtctga tcctgtttgt tctttctgta agtaaaacgg cgaaccggat
       61 ttcatctatg gatagagcat aatttacaac accttccgta tcacctgtct tatattgaaa
      121 ccggtcaagt tcggctttgg tcagggcaat gtaggctgtt ttgtattgtg gtaagatggt
      181 catcttctct ccaaggcaat aacccagcag acggagcctg ctttcagaat atgtatcata
      241 gacgtttcgg tgtatctgct ccggatcgat tcccgttttt atcaacaggg ctgatatttg
      301 aaaggtttca ggataattac atgagtaact gaatgatcct gtatcggtca ttattcctac
      361 aaaaagcccg gtagcaacct cctgtccgat caaatcagac caaccgcact gctcgataaa
      421 attgaaaagc aattccgaag tggacgagat atcgattctg gatataatat agtcaaagtc
      481 ttctgacaag ggatcaagat gatggtcaat caggacttta acagcacggg cgctttttaa
      541 agcgggtgcc atggcgtcgg tgcggtgaag tgcattataa tccaggcaaa agatcaggtc
      601 agcattataa atgagttctt tggccttggg ctccaactgg ttgtatatga ctatccggtc
      661 ggctccgggc atccatttta agaaatcagg aaagtcatta gggacaatac ccgtcacctg
      721 cggaactctc ttctccaaaa gcttgaccat ggcaataaca gaaccgatgg catctccgtc
      781 aggattgtga tggctggtca ccacaaccga atggctgtct ttaataatgc ttttgattat
      841 ttcgatatcc ggatgtttca aaaaatcgtt cttttagtgg gcaaagtaaa tgaaaatatt
      901 cctgatttcc ttatttttgc gcgctcaaac ttaaatcata tgaatggaaa tatcacatta
      961 accatgatca aaccggaggc cgtaagaaac ggtcacaccg gtcatattct cgcaaaaatt
     1021 actgaaggag gatttggaat tgttgctatg aaatatctga aattaacgcg tgaacaggct
     1081 gaaaagttct atgaagttca tcgcgaaagg cctttttata aagacctggt cgaattcatg
     1141 acatccggcc cgatcgtagc cgccattttg aagaaggaca acgctgttga agaatacagg
     1201 tcgtatattg gttcgacaaa tccgaaagac gcggccccgg gcactgttcg ggcactttat
     1261 ggaacaaaca ttcaggagaa tgccgtacac ggttccgaca gcgatgaaaa tgctgcccgg
     1321 gaagcctcct tcttcttcag cgaattggag cgttattgat cttttccgga ttccggtttc
     1381 agtacccgga aggtgccatt gcggaaaatc ataataatct gttcaaccgg ttcatcatcc
     1441 ttatgaaccg gttttttttg tatttcgtta ttctcttctg cttcaaagag agtaggcggt
     1501 aaggataccg gaacagtttt gctaactgcc ggacttgtac tcaaaccgga agtagctgac
     1561 tttttccccg aaaccagcca gttcaggtct gcctccggga aggcagtttt gattttttca
     1621 aggaagtcaa tactgggttt attcctgccg gaaaggatat gggaaatgct ggaacgctga
     1681 accccgattc tgtctgcaaa ttccgaggcg cttaatccct ggtctgccat tagttgcctg
     1741 aacctgtcga tcatgctttc attctatatt acaaatgtaa tgcaatattg tttacatgag
     1801 taatacattt gtaaacagta aatatcctcc atacgcaatc taatttactg taatacagcg
     1861 cctttctaca actaattaca attgtaattt acaaaagtaa acatttgtaa attggtagtt
     1921 atatcacatt tgtatttaaa ctaattattc cgattaggat attcaatatg ctatattcca
     1981 gatattagat gcatatatgc tttcttctgg ctttaattgc ggagtattgt tctttgtttt
     2041 ctactaagaa acacggggaa agaaaactgg aagtcaaaac gaaaggtccg ggcagaaata
     2101 tacctgcccc ttccctattt atgtgaggga gaattatctt tattaaaaaa aatatgaagg
     2161 taaattaccg tttgatgaat tttccggtaa aagttcttcc gttcgcagac acactcacaa
     2221 aataaactcc agcctcaagg ccttttgcgt cgacagtata ttcattaccc attaaagaag
     2281 ctgtgtaaat ttctcttccg gtgatgtctg taataatgat atcggcatta ttggcattga
     2341 cctgatcaag gctgactgta aacgatgtcc tgacgggatt tgggtataga tttaaactaa
     2401 cgtgttgttt gtcagggcca ttgatgaccg ttagtatatt attggccagt acaaaatcag
     2461 gaatgccgta gcccagcatg tcatcgggat tatcgtactg actggcactt ttcctgatgg
     2521 catcggcaat ttgcatgtta gtcatggctg gatttgcctg ccacagacag gcagccattc
     2581 cagccaggat gggtgatgag aaagaagtgc cgcctccaaa agtaaatcca ccgcccatcg
     2641 gttccaccac gtataccatt gatccttgtg tcgccacatc cggttttacc cggccatcat
     2701 aagaagggcc atgggagctg aaacccacat aaaaaccatt ggcgtccaca gcccctattc
     2761 ccattacgct gtcgccatca gaaggcgctg ataaatagta ccatgagcta ttgccttcat
     2821 tacccaggct atttacgacc aatattcctt ttttcgcggc catatcggcg ccttttgtgg
     2881 cgggagtcgt atttccgtcc atatcttcgt aggtatgatc ctgggtcggg tcgttgaatt
     2941 gagaatagcc cagtgatgaa ttgataatgt cggccccaac gctatcggca aattccgcgg
     3001 cagataccca gttgtattct tcgatcaagt attccgatcc accgtcctca gaccgcagaa
     3061 gccagtaatc ggccattggt gccgtcccga tcagttgccc gggcgaattt gcccccatgc
     3121 aggaaaggac cattgtcccg tgctggtgtt catcaaactg aatatcccct cccctgacga
     3181 aatcctttgt tcccaggatc tggccgttca tccagagact atcgaaagca tctacactgt
     3241 tggcattaaa atatccggca tccagaacgg ctataacctt cccctgtccg cggtaaccca
     3301 tttcgtgaaa aacatctccg ttcagcatct ggatctggtt aaatcccatc ccataatcaa
     3361 atgcaccggt ttgggcttta tttttaaagg agtttccggg cccctggtta tacatctcat
     3421 gataaaagaa cagcttctct ttatcgttct gaatcctgcc aggctgatac ttttcctgag
     3481 gataaccggt gatgccagcg acatacggca aggcatcgat tgcttccaga acatccggat
     3541 tatcggtaaa aacagtaacc
//
LOCUS       DAOQXS010000334         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27348_length_3558_cov_3.748787, whole genome shotgun sequence.
ACCESSION   DAOQXS010000334 DAOQXS010000000
VERSION     DAOQXS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27348_length_3558_cov_3.748787"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PKM34_09220"
     CDS             <1..1676
                     /locus_tag="PKM34_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83810.1"
                     /translation="LFHSSNNSNHTWEAGPSIEAYHSYRPPLTAGGSDAEYHAQKVRF
                     EYKSVIARALMLNMKTGISRINHIETDTLTYQRYDFHLMGGYNFGRGYSISFSYDYGP
                     MVNNGLYQFAGDIKNHSFNISPSMMKAYFKERINLTIFSNFIYRMDLGYISVNVNPKI
                     EAYLFNDWYAVASGTYHFTQQEYTDQTTRNSYVYAEVSIRKRFGKSDFNKWQKHTRRL
                     KVVMFKDDNGNGTKEDNEQGIPFVKTRLKLTNTDNPYVSTQFPVDIILLSNKAGAVNY
                     NKLPRGFYELTVTPLTDMKEYFYVNRSAEKLELIKNATYYVPFQKASKISGKVTVQRQ
                     KFIKTGEESLDLTNIKVTAYNKQGNSYSSFTLSDGSFTIYVPDNNSYYLRMGNVFGSN
                     FRITQNDINVDVAGGKVPEVVFNVVENSRQVKFKGAKPAVTDTAQAEPLKIKVLHGKF
                     YENSKEAAVNKNAVPVFNIPEAAVQEEPIIPGNFYVVTGSDSTRTQAVKIKRIMDENG
                     LDARLGYNESEGMYYIFTGYYPNKGDAKDELERLKAAGMADVEIIKFQE"
     gene            2051..2359
                     /locus_tag="PKM34_09225"
     CDS             2051..2359
                     /locus_tag="PKM34_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83811.1"
                     /translation="MNPAQNIVIALFLTIQLGIGENRIYIKPSVFTLPEIIFTNQANY
                     ELSKKNKIPRTDPPAIRIYHKNNVHHPEKSFQVKVTMPDLVAVFHRGNGLDNYNKKIK
                     "
     gene            2356..3222
                     /locus_tag="PKM34_09230"
     CDS             2356..3222
                     /locus_tag="PKM34_09230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ83812.1"
                     /translation="MKTRQTALITGATSGIGKAFAVCYAGMGYDLMVTGRRMDRLEQL
                     CSELSREYGIRAEAVKAELSEEEDMERLLEVIMRRDNIGILVNNAGFGSGIDFYQNSL
                     EEHLKMLDVHVKASIKLIYAVLPQMRMRKEGVIINVSSLGAFMPAPGSSMYSATKLFL
                     ASFSESLNMEVRRHGIKVRCVCPGFTHTEFHERRAQGKIRAGGKLMWMEADDVVRKTI
                     RSLKKNKIVVVPGIINRLLIAVTSIIPRRLYYHIMERSGKAKVGPGFIDRMKQLVEKP
                     VSFFFTTHTDQV"
     gene            complement(3121..>3558)
                     /locus_tag="PKM34_09235"
     CDS             complement(3121..>3558)
                     /locus_tag="PKM34_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83813.1"
                     /translation="LMRQIEYYWISGYFAKREDMMILLEKLEKWVLHIRKQAESGYKF
                     HYGQPAEGIPNSFMMYENEVVLNDNSIWVRVGDHMATYLTFDVISLLVTQDTAFCTNV
                     EQHMRQLMKKSNLISVSGEKERNRFFNKLLHTIDKARTDLGLS"
BASE COUNT         1030 a          781 c          869 g          878 t
ORIGIN      
        1 tgctttttca ttccagtaac aattccaacc atacgtggga agcaggcccc agcatcgaag
       61 cataccattc gtaccggcct ccgctgactg caggcggatc agatgccgaa tatcatgccc
      121 agaaggtccg gttcgagtac aagtcggtca ttgcacgggc tttgatgctg aacatgaaaa
      181 ccggcatcag ccgtatcaat catatagaaa cggatacgct gacttaccag cgttacgatt
      241 tccatctgat gggaggttac aatttcggcc ggggttacag catttccttc agttatgact
      301 acggaccgat ggtgaacaac gggctatacc agtttgccgg tgatattaaa aaccacagtt
      361 tcaatatcag ccccagcatg atgaaagcgt atttcaaaga gaggataaac ctgaccattt
      421 tctccaattt catctacagg atggatctgg gttacatttc cgtaaacgta aatcccaaaa
      481 tcgaggcata tttattcaac gactggtatg cggtggccag tggcacctat cattttaccc
      541 agcaggaata taccgaccag actacgcgga attcttatgt ttatgccgaa gtatcgatcc
      601 ggaagaggtt cggaaaatcg gacttcaaca aatggcagaa gcacacccgc aggctgaaag
      661 tggtaatgtt caaagacgat aacggaaacg ggaccaagga agacaatgaa cagggcattc
      721 cgttcgtcaa gaccaggctg aagctcacca ataccgacaa tccgtatgtc agcactcaat
      781 tcccggtgga tatcattctt ctttcgaata aagcgggagc ggtgaattac aacaaattac
      841 ccagaggatt ttacgaactg accgtcactc cgctgaccga tatgaaggaa tatttttacg
      901 tgaaccggag tgctgaaaaa ctggaactga tcaaaaatgc cacttattat gtcccgttcc
      961 agaaggccag caagatcagc ggcaaggtca cggttcagcg ccagaaattc atcaaaacag
     1021 gggaagagtc gctggacctg acaaatatca aagtgaccgc ctataataag caggggaaca
     1081 gctactcttc attcacgctg tcggacggca gctttacgat ctatgtccct gacaacaaca
     1141 gttattatct caggatgggg aatgttttcg gcagcaattt ccggattacg caaaacgata
     1201 tcaacgtgga tgttgccggg ggaaaagtac ctgaagtggt attcaacgta gtagagaaca
     1261 gccgccaggt caagttcaag ggggccaagc cggcggttac cgatactgcc caggcggagc
     1321 cgcttaagat caaggtgttg catggcaagt tctatgaaaa ctccaaagaa gcagcggtta
     1381 acaagaatgc cgttccggta ttcaatatcc cggaagccgc ggtccaggaa gaaccgataa
     1441 tcccgggaaa tttctatgtc gtaacaggat cggattccac ccggacgcaa gctgttaaga
     1501 tcaagcggat tatggatgag aacggcctgg atgcacgcct cggatataat gaatccgaag
     1561 gcatgtatta catcttcacc ggatattatc ccaataaagg ggatgcgaag gatgaactgg
     1621 aaaggttaaa ggcggcgggc atggctgatg tggagatcat taaattccag gaataatcct
     1681 attgcctttc ttaattttta ttaaaaatca caacttttca ccggaaatag aatccatacc
     1741 gcattttttg catttctgca aatttgaaag ttaattataa gatatagttc gtttcttccc
     1801 agacaaatgc cataaggatc aatttccctg atcggaccgg ctgatttaat attgtgattg
     1861 aatttcaaca tattaaatcg aaggccatga gaaccaggaa atttaattca atggtaaacc
     1921 gtcggcaggc cagcagtttc atctcagccc tgaggttatc gattacatgt accggtaaaa
     1981 tattgaataa aaagtgactt tcgggtccaa cttgtttcct tttaattatg acaagcaaac
     2041 aaatcagaaa atgaatccgg ctcaaaacat agtgattgca ctgttcctga cgatccagct
     2101 ggggatcggc gagaaccgga tttatatcaa accctctgtt tttacattgc ctgagattat
     2161 cttcactaac caggccaatt acgagctttc aaagaagaat aagattccca ggacagatcc
     2221 tccggctatc aggatttatc acaaaaataa tgttcatcat ccggaaaaat cgttccaggt
     2281 taaggttaca atgcctgatc tggtggctgt ttttcatcgc ggaaatggcc tggataacta
     2341 taataagaaa ataaaatgaa aaccagacag acagcattga taacgggagc cacgagcggg
     2401 atcgggaagg cttttgctgt ctgttatgcc ggtatgggat acgacctgat ggtcaccggg
     2461 aggaggatgg acaggctgga acagctttgt tcggaattat cccgggaata cgggatcagg
     2521 gctgaagcgg tcaaagccga gttgtcggag gaagaagaca tggagaggct gctggaagtg
     2581 attatgcgac gggacaatat cggaattctg gtcaacaatg ccggttttgg ttcagggatt
     2641 gatttttacc aaaacagcct ggaagagcac ctgaaaatgc tggatgtgca cgtaaaggca
     2701 tcgataaagc tcatatatgc agtattgcct cagatgagga tgagaaagga gggggtgatc
     2761 atcaatgtct cgtcgctggg ggctttcatg ccggcgccgg gaagttcgat gtattctgcc
     2821 accaagctgt tcctggccag tttctctgaa tcgctgaata tggaggtcag gcggcatggc
     2881 ataaaggtcc ggtgtgtttg tcccgggttt acccataccg agttccatga acgccgggct
     2941 cagggcaaga tcagggcggg cggcaagctg atgtggatgg aggcggatga cgtcgtgaga
     3001 aaaacgatca gatcgctgaa gaagaataag atagtggtag tgccgggaat catcaaccgg
     3061 ttattgattg ctgtgacgag catcatccca cgccggttgt attatcatat catggaaagg
     3121 tcaggaaagg ccaaggtcgg tccgggcttt atcgatcgta tgaagcaact tgttgaaaaa
     3181 ccggtttcgt tctttttcac cactcacact gatcaggttt gatttcttca tcagctgccg
     3241 catatgctgc tcgacattgg tacagaaggc cgtatcctgg gttacgagca ggctgatcac
     3301 gtcgaaagtc aggtaagttg ccatatggtc accgacgcgg acccaaattg aattatcgtt
     3361 cagcaccacc tcattttcgt acatcatgaa tgaattcgga atcccctcag ccggttgtcc
     3421 gtaatgaaac ttatatcctg attccgcctg ttttcggata tgcaagaccc atttctcgag
     3481 tttttccaaa aggatcatca tatcttcacg tttggcaaaa tatcccgata tccagtaata
     3541 ttctatctgc cgcatcag
//
LOCUS       DAOQXS010000335         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27574_length_3536_cov_3.816432, whole genome shotgun sequence.
ACCESSION   DAOQXS010000335 DAOQXS010000000
VERSION     DAOQXS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27574_length_3536_cov_3.816432"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..2283)
                     /locus_tag="PKM34_09240"
     CDS             complement(55..2283)
                     /locus_tag="PKM34_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83814.1"
                     /translation="MKFIFSYIALFTLTVLLAIPEGYGQHAPVRVVDGKTGQPVPFAH
                     VCFQPLDGTAQTHSITGEDGVVPNAVSSRSVVAVSFVGYETIYDTIDPGMPVTLSLRP
                     KIQEMNEVVVTAQYAPVRADQSIYKVKVISSKQIERKAANNLNDLLSSELNIRISQDG
                     ALGTSMSLQGLSGEHIKFLIDGVPVIGRMNGNIDISQLNLYNVDHIEVIEGPMSVVYG
                     SNALAGVINIITKENKNTRQSAYARGYVESAGVYNFDLGASRKFGDHVVSVTGGRNFF
                     DGFTDNDSLRSMRWKPKRHFFTDGYYLYARPRTKFKLSSSWFDEKLQSRGNLMPPYYE
                     TAFDSYFFTSRWTTRAEVNQKVGEHRYLNLTASYAYYLRQKETFFKDLTTLTETATTN
                     PEDYDTTRFYNSLLRGTFSKSDQKSRFNYQLGIDINLESGKGKRITENHQQIGDYAAF
                     FSIQYKPVKWMELQPGLRYIYNTNYKAPLVYSFNLKFTFLNNYSIRSSYSRGFRAPSL
                     KELYLYFVDVNHNVQGNPELKAENSHNINLSFQYNRETAKSFAGAEINLFYNNINDVI
                     TLAQSAGTLYTYINVDKYITQGFQAAVSYRLYPWATLRVGGGMTGRFNSLAEEQGVEE
                     RFYYSPDAVTTLTYKWLKYDMELTADYKFTGKLPQFYIVGDQVIEGYISAYHTVDLTL
                     QKTFLKNRFTVGAGVKNLFDNTTIPAAGGSGGVHTGGTTSYPVGWGRTYFIQASVNIN
                     KF"
     gene            complement(2287..>3536)
                     /locus_tag="PKM34_09245"
     CDS             complement(2287..>3536)
                     /locus_tag="PKM34_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83815.1"
                     /translation="SILFLNGFSQGYKAGAFESSLDSVQMGPGYANDVYYSFEEGVVA
                     VVPRTNWDIAFHTTLWSATILSNGASTINGVYLYTYPNADTAGWNTVDTVGMAGWPLL
                     YDSEEYWEEGAFVRNATGHPDYGWGKYNPISHDVVGDSIYILKTSAGQMKKLWIQRKH
                     SAANTYYIRHANMDGSDDIEQVLQINPYTTKNFVYFSFANNELIDREPDTAAWDIVFT
                     RYMAIQDNGTPYPVVGVCNNLEVYANEFYPVAPDFIDYMSMPFDSTKSPIGWEWKTFD
                     MGTFTWSVADSTAFFVHTRKDNVYKLVFTKFEGSTTGNIVFSKEALSSSAVNENIPDD
                     PVRIYPNPVRDRISVEFNRIIKGKAEIRVFDMTGREVYNTRLTADGKTFSFALPQNAD
                     MKGLYILRVSTEEGIYTTRFIAD"
BASE COUNT          899 a          885 c          806 g          946 t
ORIGIN      
        1 gaagttataa ggaaaagaaa caacatcaac aagctgatat ttaatgactt cattttaaaa
       61 tttgttgata ttcaccgatg cctggataaa atacgttcgc ccccagccga caggatatga
      121 ggtcgtccct cctgtatgaa ctccgcccga tccgcccgca gctggaatag tcgtgttatc
      181 gaataaattt ttgacaccgg cgccaaccgt gaaccggttc tttaagaaag ttttctgaag
      241 cgtcaggtcc accgtgtggt aagcgctgat atatccttca attacctggt caccaacaat
      301 gtaaaactgg gggagtttcc ctgtgaactt gtaatctgcc gtcaactcca tatcatattt
      361 cagccacttg taggtcaagg ttgtaacggc atcggggctg taataaaacc tttcttccac
      421 accctgctct tccgccagtg aattgaaccg gccggtcata ccgccgccca cccgcaaggt
      481 tgcccaagga tacaaccggt aactgacggc tgcctggaaa ccctgggtga tgtacttgtc
      541 gacattgata taagtgtaca atgttccggc ggattgggcc agcgtaatta catcattgat
      601 attgttgtaa aaaagattga tctccgctcc ggcaaacgat ttggccgtct cacggttata
      661 ctggaatgaa agattgatgt tgtgcgaatt ttccgctttc agttcaggat tgccctgtac
      721 attatgatta acatccacaa aataaagata cagttccttt aaggaaggcg ccctgaaacc
      781 ccggctgtat gaggaccgga tgctgtaatt gttcaggaaa gtaaatttca aattaaatga
      841 gtagaccagc ggggccttgt aattggtatt gtatatgtac cgcaaacccg gctgaagttc
      901 catccacttc accggtttgt actgaatgct gaaaaaggcc gcatagtcgc cgatctgctg
      961 atggttttcc gtgatccttt tgcccttgcc cgattcaaga ttgatgtcaa tacccagctg
     1021 gtaattgaaa cggctttttt ggtcactctt gctgaaagtc ccccttaaca ggctgttata
     1081 aaagcgggtt gtatcgtaat cttccggatt cgtggtcgct gtctctgtca gcgtggtcaa
     1141 atccttgaag aaagtttctt tctgcctcag gtaataggcg taggaagccg tcaggttcag
     1201 atagcggtgt tcgccgacct tttgattgac ctcggcccgg gtcgtccatc tcgatgtaaa
     1261 gaaatagctg tcgaaagccg tttcatagta tgggggcatc aggttccccc tgctctgcag
     1321 cttttcgtcg aaccacgatg acgacagttt gaatttggtt ctcggccggg catacaggta
     1381 atatccatcg gtaaaaaaat gtcttttggg cttccagcgc atcgatcgga gagaatcatt
     1441 atcggtgaaa ccgtcgaaaa aatttcttcc gcctgttaca gaaaccacat ggtcgccgaa
     1501 tttacgggag gcgcccaggt caaaattata caccccggcc gattcaacgt atcccctggc
     1561 ataagcgctc tgccgggtat ttttattttc tttggtgatg atattgataa ccccggccag
     1621 tgcatttgat ccgtatacca ccgacatcgg cccttcaatc acctcgatat gatcaacatt
     1681 gtaaagattt aactggctga tatcgatatt gccgttcatc cggccgatga caggtactcc
     1741 atcgatcaaa aacttaatat gctccccgga aaggccctgc aggctcatgc tcgtacccag
     1801 ggcgccatcc tgcgagattc tgatattcaa ttcgctgctc agcaggtcat tcagattatt
     1861 ggcggccttc cgctcaattt gcttgctgct gatcactttc accttgtaaa tggactgatc
     1921 ggcgcgaacc ggggcatact gggccgttac gaccacctca ttcatttcct gaattttcgg
     1981 ccgtaacgat aaagtgaccg gcatcccggg atcaatggta tcgtaaatcg tttcataacc
     2041 cacaaaagat acggccacca ccgacctgga tgataccgca ttggggacaa ccccgtcttc
     2101 gcctgtaatg gaatgggttt gcgccgttcc gtcgagtggc tgaaaacaaa catgggcaaa
     2161 cggaaccggc tggcccgttt taccatcaac aaccctgacc ggagcatgct ggccataacc
     2221 ttccggaatg gccagcagca cggtcaatgt aaacaacgct atataggaaa aaataaactt
     2281 caacgcttaa tcggcaataa aacgggtggt ataaatgcct tcttccgtac tcaccctgag
     2341 gatgtataaa cccttcatat ctgcattttg aggcaatgca aacgaaaagg ttttgccatc
     2401 ggctgtcagc cgggtgttat aaacctcgcg ccccgtcatg tcgaataccc ggatctctgc
     2461 ctttcctttg ataattctgt tgaattcaac gctgatcctg tccctgacag gattcgggta
     2521 aatccggacc ggatcgtccg gaatattttc gtttacggcg gaagatgaaa gggcctcttt
     2581 gctgaatacg atattccccg tggtactgcc ttcaaatttt gtgaatacca gtttataaac
     2641 attatccttg cgcgtatgga caaagaaagc ggtggaatcg gcgacactcc atgtaaatgt
     2701 ccccatatcg aatgttttcc attcccagcc gatcggcgac ttggtggaat cgaacggcat
     2761 actcatataa tcgatgaaat caggcgcgac cgggtaaaat tcattcgcat aaacttccag
     2821 gttattgcat acgcctacta caggatatgg ggttccatta tcctggatcg ccatgtaacg
     2881 tgtaaacacg atgtcccatg ctgccgtgtc aggctccctg tcaatcagtt cattattggc
     2941 aaaggaaaaa tagacaaagt ttttcgtcgt atacggattg atctgcagca cctgctcgat
     3001 atcgtcgctg ccatccatat tggcatggcg gatgtaatag gtattggcag ccgaatgttt
     3061 gcgctggatc cataatttct tcatttgtcc ggcactggtt ttcaggatgt aaatggagtc
     3121 cccgaccaca tcatgcgata tgggattgta ttttccccat ccataatcgg gatggccggt
     3181 tgcattgcgt acaaaagcgc cttcttccca gtattcttct gaatcataca aaaggggcca
     3241 tccggccatt ccgactgtat ccaccgtgtt ccatcccgcc gtatcggcat tcggataggt
     3301 ataaagatat actccattga tggtggaagc gccgttgctc agaatcgtgg ccgaccagag
     3361 cgtcgtgtga aaggcaatgt cccagttcgt tcggggcaca acggctacaa cgccctcttc
     3421 aaaactgtaa taaacatcat tggcataacc gggccccatc tgaaccgaat ccaggctgct
     3481 ctcaaaagcg cctgctttgt aaccctggct gaatccgttc aaaaataaaa tcgata
//
LOCUS       DAOQXS010000336         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27576_length_3536_cov_3.457053, whole genome shotgun sequence.
ACCESSION   DAOQXS010000336 DAOQXS010000000
VERSION     DAOQXS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27576_length_3536_cov_3.457053"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..605
                     /locus_tag="PKM34_09250"
     CDS             195..605
                     /locus_tag="PKM34_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83816.1"
                     /translation="MTKVVSLDVKCPHCDASFMDEGHLINNKASIKLLIHTPIGQKGF
                     IWLSSIYGDYNFISEFQIKEGEVVEFFCPVCKESLKRKKIECDVCNAPIVSLNCTIGG
                     RVNICSRSGCKNHYIVFEDLDTTIRKFYEDYGYH"
     gene            complement(627..1649)
                     /gene="hypE"
                     /locus_tag="PKM34_09255"
     CDS             complement(627..1649)
                     /gene="hypE"
                     /locus_tag="PKM34_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNQ83817.1"
                     /translation="MMLHNDTILLGHGSGGKLSHDLIGDLFVRHFHNIHLEKQTDSSI
                     LNIESGKIAFTTDSFVVDPIFFPGGDIGKLAVCGTVNDLAVSGARPLYLSAGFIIEEG
                     FLYTDLEKIVVSMADEARKAGVMIVTGDTKVVDRGKCDKVFINTSGIGILSERYSHIS
                     DGSKIRPGDKVIINGPVGEHGMAVLSARKDLNIKSSIVSDCASLNRMIAVVLEATDGV
                     RFMRDATRGGLATVLAELAEKNQLGISIEEERLPVTSQVRSFCELLGFDPLYVANEGK
                     VVIISSKESADKVILALKKSEYGKGAEIIGEICGDYPGKAYLNTIVGGRRIIDMLAGE
                     QLPRIC"
     gene            complement(1657..2736)
                     /gene="hypD"
                     /locus_tag="PKM34_09260"
     CDS             complement(1657..2736)
                     /gene="hypD"
                     /locus_tag="PKM34_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNQ83818.1"
                     /translation="MRFVDEYRDRALVLKLAENIRSISRRKIALMEVCGGHTMSIQRF
                     GIPALLPPTISLLSGPGCPVCVSSRRYIDLAIALSRIPGVIITTYGDLIRVPGSSTSL
                     DKEKSKGADIRIVYSPMDALAVAQKNPTAEIVFLGIGFETTAPASAAAIIGAERYGLS
                     NFYLLSTHKIMPPAMAALIDEGVKIDGYIAPGHVSAITGTSIYTDVAERYRLGCVIAG
                     FEPVDLMESILMLVRQIEESSQRIEIQYKRAVPAEGNPQARKLMDQVFELRDEWWRGL
                     GILPLSGLKLKNQYSRYDAEKVFGVEPEPTKEDKGCICGEILKGLKTPKECKLFGTAC
                     TPVNPAGPCMVSNEGACHAFFRYNR"
     gene            complement(2739..3230)
                     /locus_tag="PKM34_09265"
     CDS             complement(2739..3230)
                     /locus_tag="PKM34_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ83819.1"
                     /translation="MNRTLYLLATAILAILSLTACRTEESKRQETIRELMDIDRRFSD
                     LSLEKGSHEAFLHYIDDSCVLLRPNRNPIIGRANIEALYKVPDTTFILTWDPLSGDVS
                     RSGDMGYTYGIFTVKMDSPAGEKVEKKGTYVTIWKKGRDGNWKFVLDTGNQGIGSPNS
                     VSD"
     gene            complement(3280..>3536)
                     /locus_tag="PKM34_09270"
     CDS             complement(3280..>3536)
                     /locus_tag="PKM34_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNQ83820.1"
                     /translation="HLTVKDDLSNMSTTVRSESGINKVVLSGGTFQNRFLLKETARTL
                     NSHGFTCCFPEMIPANDGGIALGQLVIASARRKKMFIPDL"
BASE COUNT          901 a          853 c          804 g          978 t
ORIGIN      
        1 ccaggctttt tgtaactgat cggatctggt tgtccgcatc cgcaagttcc gcacatattc
       61 ttattttttg agcaaaggta agcattatca tcaggataga attgttctaa attgcaaatt
      121 tcttactgta ttccaaattt tgattaggaa aaatagtcaa taatcaatat atttgtttta
      181 gcaaatctat gatcatgaca aaagttgttt cattagacgt taaatgcccg cactgcgatg
      241 cctccttcat ggatgaaggt cacctgatca ataacaaagc cagcattaag ttgttgatac
      301 atactcccat cggacaaaag ggatttattt ggctaagctc aatttatggt gattataact
      361 ttatttcaga gttccagatc aaggaagggg aggtcgttga attcttctgc cctgtttgca
      421 aagaaagcct aaagaggaaa aaaatcgagt gcgatgtgtg taatgctccg attgtcagcc
      481 tgaattgcac tatcggaggg cgggtgaata tttgcagccg cagtggatgt aaaaaccatt
      541 acatcgtttt tgaagacctg gatacaacga tccggaagtt ttacgaagat tatggatacc
      601 attgatcgct gatcaccggg ttcggttcag cagatcctgg gtaactgttc gcccgccagc
      661 atatcgatga tccttcttcc cccgacgatg gtgttcaaat aggccttgcc gggataatca
      721 ccgcaaatct ctcctataat ttctgccccc ttgccatatt ctgatttttt cagtgcaaga
      781 attaccttgt cggcagattc ttttgatgaa atgattacta ctttgccttc attggcaaca
      841 taaagcggat cgaatcccaa taattcacag aaagacctga cctgggaagt cacgggcaat
      901 ctttcctctt caatacttat acccagttga tttttttctg ccagttcggc cagaacagtg
      961 gccaggcctc cccgggtggc atcacgcatg aaccgcacac cgtcggtggc ttccagcaca
     1021 acggcaatca tccggttcag cgatgcgcaa tctgaaacaa tactgctttt gatattgaga
     1081 tccttccggg ccgataaaac ggccatccca tgctctccaa ccggcccgtt aataatgact
     1141 ttgtcgccgg gccgtatttt actcccgtca cttatgtggc tgtacctctc gctgaggatg
     1201 cctattccgg aggtgttgat gaacacctta tcacatttgc ccctgtcaac taccttcgta
     1261 tcgccggtta cgatcattac tcctgccttt cgggcttcat cggccataga gacgacaatc
     1321 ttctccagat ctgtataaag aaaaccctcc tcgataatga atccggcact aaggtaaagt
     1381 ggccgggccc cggatacagc caggtcgttg accgtgccgc atacagccag tttgccaata
     1441 tcgccgcccg ggaagaaaat tggatcgacg acaaaggaat cggtcgtgaa tgcgattttc
     1501 ccgctttcaa tattcaaaat actcgaatcg gtctgctttt ccaggtgaat attgtgaaag
     1561 tgacggacaa acaggtcacc gataaggtca tggctcaatt tccctccgct tccgtgaccg
     1621 agtaaaatag tatcattatg caacatcatt cgggagttat ctgttataac ggaaaaaagc
     1681 atggcaagct ccttcattgg agaccatgca ggggccggcc ggattgaccg gggtgcaagc
     1741 ggtgccgaaa agcttgcact ctttcggcgt ttttaatcct ttcaggatct ccccgcagat
     1801 acatcccttg tcttctttcg taggttccgg ttccacgccg aaaacttttt cggcatcata
     1861 acgggaatat tgattcttca gtttcaaacc gcttaatggc aatataccca gcccgcgcca
     1921 ccactcgtcg cgaagctcaa aaacctggtc catcaacttt cgggcctggg ggttaccttc
     1981 cgccggtacg gctcttttat actgaatctc aattctttgt gaggattcct cgatttgtct
     2041 gaccagcata aggatcgatt ccatcagatc gacaggttcg aatcctgcga taacacagcc
     2101 caatcggtat ctttcagcca catcggtgta gatcgaggtg cctgttatcg cgctaacgtg
     2161 gcccggggcg atataaccgt cgattttgac cccttcatcg atcaatgctg ccattgccgg
     2221 gggcataatc ttatgggtgc taagcaggta aaaattactg agaccgtatc tttcggcgcc
     2281 aatgatcgcc gctgcgctgg caggggccgt ggtttcaaaa cctatgccca ggaatacaat
     2341 ttccgcagtc ggattttttt gtgcaacagc cagggcatcc attggagaat aaacgatccg
     2401 gatgtccgca ccttttgatt tttctttatc aagagaagta gaagacccgg gtacacggat
     2461 gagatctccg tatgtagtaa tgattacgcc cggaatcctt gataatgcta tggccaggtc
     2521 gatataacgc ctgctggaaa cgcaaaccgg gcagcccggg cctgacagca gggagatggt
     2581 cggtggtaac aatgccggga ttccgaatct ttggatggac atggtatgcc cgccacagac
     2641 ctccattaaa gctatcttcc gcctggaaat gcttcggatg ttctccgcca gtttgagtac
     2701 cagggcgcgg tccctgtatt catcaacaaa cctcattttt aatcgcttac actattaggg
     2761 gagccgattc cctgattgcc ggtgtcgagc acaaatttcc agttgccatc tctgcctttt
     2821 ttccagatgg tgacataggt tcctttcttt tcaacttttt caccggcagg agaatccatt
     2881 tttaccgtaa aaatgccata tgtatagccc atatcacccg acctggaaac atcgccggag
     2941 agaggatccc atgtaaggat aaatgtagtg tcaggtactt tatataaagc ttcaatattg
     3001 gcccggccga taatcggatt ccggttaggt ctgagtaata cgcagctgtc gtcgatgtaa
     3061 tgtaaaaaag cttcatggga acctttttcc agagaaagat cggagaagcg gcggtcgata
     3121 tccatgagtt cacggatggt ttcctgccgt ttactctctt ccgtccggca ggcagttaag
     3181 cttagaatgg cgaggatggc ggtggcgagc agatacagag ttcggttcat acgatttggg
     3241 atagcttttc agtcatataa aaatatataa aatgatcgat tataaatccg gaataaacat
     3301 ttttttccta cgggctgatg caataaccaa ttgcccaagg gcaatacccc cgtcattggc
     3361 aggtatcatt tccgggaaac agcatgtaaa accatgactg tttaatgttc tggcagtttc
     3421 tttcagcaga aacctgttct gaaaagtgcc accggatagc actactttgt tgatgcctga
     3481 ttcagacctg acggtcgtgg acatgttgct caggtcatct ttaaccgtca ggtgtg
//
LOCUS       DAOQXS010000337         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_27840_length_3511_cov_3.379630, whole genome shotgun sequence.
ACCESSION   DAOQXS010000337 DAOQXS010000000
VERSION     DAOQXS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27840_length_3511_cov_3.379630"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PKM34_09275"
     CDS             <1..2070
                     /locus_tag="PKM34_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83821.1"
                     /translation="VLPGANVSQASNNRIGIVWTGGGGGYNINGTLCTLIMHYNCGST
                     VLDGIEGIDTMSIVTPLFEEIPYTYINGSVSAGIYTINTYYVDAARPSSGDGLSWGTA
                     KKTIQEAANLSLKPGEQVLIKPGTYAETVTIKSNGGYLLRPTTGVVLSDNNKITFPSG
                     AVLPCIDLVNYPGQYYAYIYRSWVSNNGYYKITEVNTGSNFIRVDGASFIPESGTVNN
                     YGKVMAAIGRPIIFKKDPAASESQRVVVNTTTLGTIADAFYVGTPISPDFTADSANWH
                     IFEGIDITGGNGTKGLHIQCSSQNVFAKGKIYSSGTSGTNNLGVIISGNSSRGARFNI
                     IQNNEIYNTPYQGILIGYTNNSASYNFSHNNHVLDNNMYITGSTFSARFNNAVKIQQF
                     NKNNVIEGNNFHDIPIYTAGEAAVAVVTKADSTLIYNNIFRNIGKSASNSGVNAAIML
                     DSTIYKAHVYNNIIYNDDTVTNAVYAFRVYGRRHVGSKLAFNTVYKIDNAFYLQDNNA
                     NGAAIDFGIHNNIISPTVGYFNNVGTTGRFTVTYNLFRISPGTPYASGTGNIFGDPLF
                     IDPNGSSMYGLMLTPTSPAMDTGTVITNLTRDYIGDLRGSPEPTLGAFENTMTATWNG
                     SVSTDWNNVNNWTYKILPKNYSPVVIPNVTNDPVVSTGNATCNSISLSPGATLRVSPP
                     RTLTVNY"
     gene            2163..>3511
                     /locus_tag="PKM34_09280"
     CDS             2163..>3511
                     /locus_tag="PKM34_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ83822.1"
                     /translation="MSKNKRKYLLILGLVLLLIPAYFLFASANPKTAERFPFLVFLLA
                     VDFYFWYSIKDQFKKLNKVLKTILSVIYWLPLGAFVVLLALTSVHKYNLTHPALVSYI
                     FGFVMVIYLAKLITVIFFLFADIFRISLFTVRHARAKKTGRPIDHGTHMSRGKFIRTM
                     GLATGGLFFSGMLIGMVRWAHDFRVRRENLFLPNLPKSFNGLRIVQISDLHLGSWASA
                     DPIAEAASIINDLDPDVVLFTGDLVNYSTNEAYRFRNELGRIRARHGVFAILGNHDYG
                     DYVTWPDETAKLQNLESLYQFFDEIGWRLLRNENQVLERNGDKIAIIGVENWSASKRF
                     PKLGNMTKAISGTENIPVKILMSHDPTHWEKEISHKFNDIDLTLSGHTHGFQFGVELK
                     NFRWSFAQYVYKYWAGLYESTHKGKKQYLYVNRGLGMIGYPGRVGILPEITLITLNS"
BASE COUNT          944 a          844 c          789 g          934 t
ORIGIN      
        1 gtccttccgg gtgcaaatgt ctcccaggct tcaaacaaca ggatcggtat tgtctggaca
       61 ggcggcggcg gcggatacaa tataaatggt acgctatgca cccttattat gcactacaac
      121 tgcggttcaa ccgtcctcga cgggattgaa ggaatcgata ccatgtcgat cgttacgccg
      181 ttgtttgaag aaattccata tacctacata aacggctctg tttcagccgg catttatacc
      241 ataaacacct attatgtcga tgccgctcgt ccttccagcg gtgatggatt aagctgggga
      301 acagcaaaga aaacgataca ggaagcagca aacctgtctt taaagcccgg tgagcaggtg
      361 ctgatcaaac ccggtactta tgctgaaacc gtaacgatta aatcaaatgg cggctacctg
      421 ctcaggccga ccaccggtgt tgtgctttcg gataacaata aaatcacatt tcccagcggt
      481 gcggtattgc cctgcatcga cctggttaat tatcccgggc aatattatgc ctatatatac
      541 cggagctggg tgtcgaataa cggatactat aaaataacgg aagtcaacac tggttccaat
      601 tttatcaggg tcgacggggc ttccttcatt cctgaatccg gaacggtaaa taattatggc
      661 aaggtaatgg ccgctattgg ccgcccgatc attttcaaga aggatccggc tgcttctgag
      721 agccagcgtg tagtggtcaa tacgacaact ctcggtacaa ttgctgacgc cttttatgtt
      781 ggaacgccta tcagcccgga tttcacagcc gattcggcca actggcatat tttcgaggga
      841 attgatatta caggggggaa tggcaccaaa gggcttcata ttcaatgctc atcccagaat
      901 gtttttgcca aaggaaagat atattcatcg ggaacttccg gtaccaacaa cctgggggtg
      961 atcatctccg gtaacagcag ccggggagcc cggttcaaca ttatccagaa caacgagatc
     1021 tacaatacac cataccaggg aatactgatc ggttatacta ataactcagc atcttataac
     1081 ttcagtcata acaatcatgt gctggataat aatatgtaca ttacgggaag tacgttctca
     1141 gccaggttta ataatgctgt aaaaattcaa cagttcaata aaaataacgt cattgaagga
     1201 aataatttcc atgatatccc tatttatacc gccggtgaag ctgctgttgc cgtagtcacc
     1261 aaggccgaca gtacactgat ctacaataat atcttcagaa atatcgggaa atctgcgtct
     1321 aattcaggcg ttaatgccgc catcatgctc gacagtacca tttataaggc tcatgtttac
     1381 aataatatta tttataatga tgatacggta accaatgcgg tttatgcatt ccgtgtctac
     1441 ggccggcggc acgtcgggag caagctggct ttcaatacag tatataaaat tgataatgct
     1501 ttttacctgc aggataataa tgccaatggc gctgccatcg atttcggcat acataataat
     1561 attatcagtc ctaccgtcgg ttatttcaat aatgtcggga ccacgggccg gtttaccgtg
     1621 acatacaact tattccgcat atctcccgga acgccctatg cttcaggaac cgggaatatt
     1681 ttcggcgatc ctttattcat cgatcccaac ggcagcagta tgtacggcct gatgctcacc
     1741 ccgacttcgc cggccatgga caccggtaca gtgatcacca acctgacacg cgactatatc
     1801 ggcgatctca ggggaagccc ggaacctaca ctcggcgcat ttgaaaatac tatgaccgcc
     1861 acctggaacg gttcggtcag caccgattgg aataatgtga ataactggac ttacaagatt
     1921 ctcccaaaga actattctcc tgtcgttatt ccgaatgtta ctaatgatcc ggtcgtttca
     1981 accggcaatg ctacatgtaa ttccatcagc ctttcacccg gagcgacgtt gcgtgtgtct
     2041 cccccaagaa cccttaccgt caattattaa atattcttaa atttattcaa atcggataaa
     2101 tatttaaaat tttcaataca tactttttat acttttgcgc cctcaattaa cggataaggg
     2161 ttttgtcgaa aaataaaagg aagtatttac tgattttggg gctggttctg ctcctgattc
     2221 cggcttattt tctttttgcc agcgccaacc cgaaaacggc cgaacgattc ccgttcctgg
     2281 tctttctcct ggccgtcgat ttctacttct ggtattcgat caaagatcag tttaaaaaac
     2341 tgaataaagt actgaaaacc atcctgtcgg tgatatactg gttaccactg ggagcattcg
     2401 tcgtcctgct ggcgctcacg tctgttcata aatataatct cacacatccg gcgctggttt
     2461 cgtacatctt tggttttgtt atggttattt acctggcaaa actgatcacg gtgatctttt
     2521 tcctttttgc cgatatattc aggatcagct tgtttactgt caggcatgcc agggcaaaga
     2581 agaccggccg gccgattgat cacgggaccc atatgagccg gggaaaattt atccggacga
     2641 tggggctggc tacgggaggg ctttttttta gcggtatgct gatcgggatg gtgcgctggg
     2701 cccacgactt cagggttcgc cgtgaaaacc tcttcctgcc gaacctgccc aaatccttca
     2761 acggtcttcg aatcgtccag atctcggacc tgcatctggg aagctgggca tcggccgacc
     2821 ctattgcaga agccgccagt attatcaatg acctggatcc cgacgtggtc ctgtttacgg
     2881 gcgacctggt caactacagc accaatgaag cctaccggtt ccgcaatgaa ctgggccgga
     2941 tcagggcccg gcacggcgtt tttgccattc tgggcaatca cgattatggc gattatgtta
     3001 cctggccgga tgaaactgcc aaattacaga acctggaatc tttatatcaa tttttcgacg
     3061 agatcggctg gcgtctgctc cggaatgaaa accaggtact ggaaagaaat ggagataaaa
     3121 ttgccatcat cggggtggaa aactggagcg ccagcaaacg gtttcccaaa ttgggtaata
     3181 tgaccaaagc aatctcaggt accgaaaata ttccggtaaa gatcctgatg tcgcacgatc
     3241 cgacccattg ggaaaaagag atcagccata aattcaacga catcgatttg accctttcgg
     3301 gtcacaccca tggttttcag tttggcgtcg aattgaaaaa cttccggtgg agttttgccc
     3361 agtatgttta caaatactgg gcgggacttt atgaatcgac ccataaagga aaaaaacaat
     3421 acctatatgt caaccgcggc cttggaatga ttggctatcc cgggcgtgtg ggtatcctcc
     3481 ctgagattac cctgattaca ctgaattctt a
//
LOCUS       DAOQXS010000338         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_28057_length_3493_cov_2.526178, whole genome shotgun sequence.
ACCESSION   DAOQXS010000338 DAOQXS010000000
VERSION     DAOQXS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28057_length_3493_cov_2.526178"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKM34_09285"
     CDS             <1..389
                     /locus_tag="PKM34_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83823.1"
                     /translation="NLENFYKALKPGGIIAVSVPNKWWIFETHGANIPGLNKIPWNRV
                     PLFSWLPRPVHEKFANARIYTKRRIRKLLQKQGFHVLQTDYVTAPMDVLPEGGLKRFV
                     IRYLFNTDTTRLPFKATSIMVFAIKK"
     gene            465..614
                     /gene="rpmH"
                     /locus_tag="PKM34_09290"
     CDS             465..614
                     /gene="rpmH"
                     /locus_tag="PKM34_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ83824.1"
                     /translation="MKRTFQPSVRKRKNKHGFRERMSTKNGRKVLSNRRATGRKKLTV
                     SDEKR"
     gene            742..2985
                     /locus_tag="PKM34_09295"
     CDS             742..2985
                     /locus_tag="PKM34_09295"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNQ83825.1"
                     /translation="MKKIIFPMVFFSFLIQTVNFAQSTIIIQPGPEGKDAQLWSITPD
                     LNIGNTPKFLASGWTHGGEPGINRALIDFDLSEIPENAVITSAKLNLYFLCLEPNFMG
                     HTGQNSATLSLVTEPWDEATVTWNNQPATTADGQVHLPQSEAPYIDYTDIDITGLIQS
                     LLTSPEEYHGIMMRLQNENPYRCILFASGDYSDTISMRPRLEITYTSESNTIVIQPGQ
                     EGKDALVWSAYPLYHFGNSPKFTCMAWSHYKEICTTRSMVEFDLSVIPANATITDARL
                     SLYFVSQENLIEGHTGENESVLRLITEPWEEDDVTWANQPETTTDFQVNLPASTDPYQ
                     DFTNIDVTNLISKQFEQPGQYHGLLLRLADESIYKCLLFASGDYAESAELHPKLEVSY
                     TVSTVTIEIQPGEEGKDAQISTIVPGNNYGNSPIFKAAAWTHSGEPGTNRALVEYDLS
                     VLPENAVIEDARLNLYFANKEPTFYGHTGENDAWLQLITEPWEEMEVTWNNQPATTSQ
                     YQVYIPQSVEEFQDYTNIDVTEVITRLYNEPETYHGLLFRLIHEEYYRCLLFASSDYT
                     DTVENRPKLVITYSICQDPIAVYTFEKEDFSVYFHDQSLHGESLYWDFGDGYYSDLNE
                     PWHIYDNPGTYEVCLTVWNDCGVDSVCHMINIEQNAEPSDTYDNTISIYPNPAVHYFN
                     VTSTVEEEVSVLLLSPNGEAIVKRIENIDPDNGITIETSKLNPGIYFLKISGPSGHSI
                     KKIMVNH"
     gene            3103..>3493
                     /locus_tag="PKM34_09300"
     CDS             3103..>3493
                     /locus_tag="PKM34_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83826.1"
                     /translation="MIKSDALSFKKLLPVIVALAVFFLIIIIYFYPVLEGKKLSASDM
                     VHFKGMSKEVVDYRDKTGEEALWTNGMFGGMPAYQISVHYTQNLMTYIHRLLRLGFHL
                     PVGMIIIYFIGFYILMRVLKINPWLSIT"
BASE COUNT         1040 a          802 c          752 g          899 t
ORIGIN      
        1 aaaacctgga gaacttttac aaagccctta aaccgggcgg aatcatcgcg gtttcggtcc
       61 ccaataaatg gtggatcttt gaaacgcatg gtgcgaatat acccggtctt aacaaaatcc
      121 cctggaacag ggtcccgttg ttctcctggc tgccccggcc tgtacatgag aagttcgcaa
      181 acgccaggat atatacaaag cgcaggatca ggaagctgtt gcaaaaacaa gggttccatg
      241 tgctgcaaac agattacgtg actgctccta tggatgtttt acctgaagga gggctgaaaa
      301 gattcgtcat caggtatctc tttaataccg atactacgag gctgcctttc aaagcaacct
      361 ccattatggt tttcgccatt aaaaaataaa ttacaaagtc gggaggattt tatttggtaa
      421 atcaaaatta aattgtactt ttgcacctcc aaaattgaga agtgatgaaa agaacatttc
      481 agccatcggt aaggaaacgc aagaacaaac acggtttcag ggaaaggatg tcgaccaaga
      541 acggccgcaa ggttctttca aacagaagag ctaccggcag aaaaaaactg accgtatccg
      601 acgaaaagag atagaatcct tttaaaatat caggaacccg aaaagaagat aatcatggtt
      661 atcttctttt tttttgccag tgcaactcat cgtataaaat tcctgtctat atttatagcc
      721 ttacctgaaa aaccagacac catgaaaaag attatttttc cgatggtttt cttttcgttc
      781 ttaatccaaa ccgtcaattt cgcacaaagc acaatcatta tccagcccgg cccggaaggg
      841 aaagatgctc agttgtggag catcacgcca gatctcaata ttggcaatac tcccaaattc
      901 cttgcctcag gctggacgca cggcggtgaa ccgggcatca acagggccct gattgatttc
      961 gacctgtctg aaataccgga aaacgccgtt attaccagtg caaagctgaa cctctatttt
     1021 ctttgcctgg aacccaattt catgggccat acaggccaga acagcgctac gctgagtttg
     1081 gtcaccgaac cctgggacga ggccacggtg acctggaaca accagcccgc cacaactgca
     1141 gacggtcagg ttcatctgcc ccagagcgaa gctccttata tagattatac ggatattgat
     1201 ataacggggc ttatccagtc actgctgaca tcgccggagg aatatcacgg gatcatgatg
     1261 cggttgcaaa atgaaaatcc ttaccggtgt atactttttg cttcgggtga ttattccgat
     1321 acgatatcta tgcgtccccg gctggaaatc acctatacca gcgaatcaaa cactattgtc
     1381 attcaacccg gacaggaagg taaagacgca ctggtctgga gcgcataccc cttgtatcat
     1441 ttcggcaaca gccccaaatt cacctgcatg gcctggtcgc actacaaaga aatttgcaca
     1501 acccgttcga tggtagaatt tgatttatca gtcatcccgg caaatgccac catcaccgat
     1561 gcacgcctca gtctgtactt tgtcagccag gagaacctga tcgaagggca taccggtgaa
     1621 aacgaaagtg ttctccggtt aattacggaa ccctgggaag aggatgatgt aacctgggcc
     1681 aaccaaccgg aaaccacaac ggatttccag gtcaacctgc cggccagtac cgatccttac
     1741 caggatttta caaatattga cgttacaaac ctgatttcca agcaattcga acaacccgga
     1801 cagtatcacg gattgctgct gcgcctggca gacgaaagca tatacaaatg tcttttgttc
     1861 gcatcaggtg attatgcaga atcggcggaa ctacacccga aactcgaagt atcatacact
     1921 gtatcaaccg tcaccataga gatccagccg ggagaggaag ggaaagacgc tcagatcagc
     1981 accattgttc ccggtaataa ttatggtaat tcacccattt tcaaagcagc cgcctggacg
     2041 cattccggag aaccaggcac aaacagggcg ctggttgaat acgacctttc cgtattgccg
     2101 gaaaatgcgg tgattgaaga cgcaagactc aatctctatt ttgctaacaa agaacctaca
     2161 ttttacggcc atacaggtga gaacgatgcc tggctgcaac tgatcacaga gccctgggaa
     2221 gagatggaag ttacctggaa taatcaaccg gcaacaacca gccaatacca ggtttacatt
     2281 cctcaaagtg ttgaggaatt ccaggactac accaatattg atgtaactga agtcatcacc
     2341 aggttgtaca acgagcctga aacttaccac gggctgttgt tcaggttgat ccatgaagaa
     2401 tattaccgct gcctgctttt tgcatccagc gattataccg acacagttga gaacaggcct
     2461 aaactggtga tcacctattc catctgccag gatccgattg cagtttatac atttgaaaag
     2521 gaagattttt cagtatattt ccatgaccag tcgcttcacg gtgaatcttt gtactgggat
     2581 ttcggcgatg gttattattc ggatcttaat gaaccctggc atatctatga taatccggga
     2641 acatacgagg tatgcctaac ggtatggaat gactgcgggg tggactccgt gtgccatatg
     2701 atcaacattg aacaaaatgc agaaccatca gatacatatg ataataccat atccatatac
     2761 cctaatcctg cggtacatta ctttaacgtt acatcaaccg ttgaggaaga agtaagtgtc
     2821 ttattgctga gtccaaacgg ggaagctatt gttaaacgca ttgagaacat cgatcctgac
     2881 aacgggataa caattgaaac gagcaaactg aatccgggaa tttactttct gaaaatatca
     2941 gggccttcag gtcattctat taagaagatc atggtaaatc actgatatta agaattgata
     3001 tcctgtaact tacaaacagg tacatttaat ttaaaataag attaaccgaa tttctttaat
     3061 ttcgcgaggt taatacgtgt attaatcaat acctgtatcc agatgatcaa gtcagatgct
     3121 ttgagtttca agaagttact cccggtcatt gttgccctgg ccgttttttt cctcatcatc
     3181 attatttatt tttaccctgt cctggaaggt aaaaaactgt cggccagcga tatggtccat
     3241 tttaaaggga tgtcgaagga agttgttgat taccgcgata agaccggtga agaggcacta
     3301 tggacaaatg gaatgttcgg tgggatgccc gcttatcaga tctccgttca ctacactcaa
     3361 aatttgatga cttatatcca tcgcctgcta aggttaggtt tccatttgcc ggtaggtatg
     3421 attatcatct atttcatagg attttatatt ctgatgaggg tattgaagat caatccctgg
     3481 ctcagcatta ccg
//
LOCUS       DAOQXS010000339         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_28521_length_3454_cov_3.768167, whole genome shotgun sequence.
ACCESSION   DAOQXS010000339 DAOQXS010000000
VERSION     DAOQXS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28521_length_3454_cov_3.768167"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKM34_09305"
     CDS             <1..1358
                     /locus_tag="PKM34_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNQ83827.1"
                     /translation="SENPEKYFDQVIEINLTELEPHINGPFTPDLATPVSKFAREVEK
                     NGWPEKLEVGLIGSCTNSSYEDITRAASVARQAIDKKLKAKSEFTITPGSEQVRFTAE
                     RDGYLDIFEKIGGVVLANACGPCIGQWARHNAHKQEKNSIMTSFNRNFAKRNDGNPNT
                     HGFVASPEIVTAFAIAGTLKFNPVTDKLLNEEGKEVSLDEPVGIDFPVNGFAVEDNGY
                     QPPAEDGSAVNVIVDPKSDRLQLLAPFPAWNGEDLTGLHLLIKARGKCTTDHISMAGP
                     WLKYRGHLDNISNNLLIGAINDFNGKTNLVKNQMTGEYGEVPAVARFYKANGTGSIVV
                     GDENYGEGSSREHAAMEPRHLNVRAILVRSFARIHETNLKKQGMLALTFANPSDYDKI
                     KEDDRMDILGLKEMAPGKPLSIVLRHADGSVDQFPANHTYNKNQIEWFKAGAALNLFR
                     E"
     gene            1449..2525
                     /gene="porQ"
                     /locus_tag="PKM34_09310"
     CDS             1449..2525
                     /gene="porQ"
                     /locus_tag="PKM34_09310"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNQ83828.1"
                     /translation="MRTLIHYFILVLPLVFILEQAGGQVGGKGTYQFLNLPNSARIAA
                     LGGNFLSINDDDITLTLANPSLINEDMHNQLGFSFVDYYTDVNYGFVQYGRTFNKAGS
                     FVGTMQYIDYGKFQEADEAGNQYGTFHAGEYALNIGWGRKLTPRWAIGANFKMIYSGL
                     ASYHSFGLGVDVAGSYFSKDELFDASLLFRNIGYQVVAYQNGNHEPLPFEIQAGISKK
                     FEHIPFRIIALVTHLEKWDVRYESSVNPKGGLDPVTGEPNKISGVDEFADNLMRHVIF
                     GGEVTIAKTVSLQGSYNYQRRQEMKIPGKLSTVGFSWGIGIRVKQFQFNYARSAYHLS
                     GSPNFITVMIDMDGFPKKHQPQSN"
     gene            2541..>3454
                     /gene="recF"
                     /locus_tag="PKM34_09315"
     CDS             2541..>3454
                     /gene="recF"
                     /locus_tag="PKM34_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNQ83829.1"
                     /translation="MYLQKLSVTNFKNYSEATLHFSDKINCFIGDNGAGKTNLLDAIH
                     YLSFCKSYFNPIDTQNIRHHEDFFSLHGTYLKNGDSPDLVSCIQRSEQRKVFKINQKE
                     YERLADHIGLFPLVMVSPYDRDLINEGSEVRRRYLDGVISQFDKQYLDDLIRYNKALL
                     SRNSLLKQASEGKALNHSLIELLDMQLADYGQKIHEKRAGFLEAFSTVFQKYFAFISE
                     GKESVTLHYSSQLFSRSLGELLAVSLDKDRSARFTTQGIHKDDLEFRISGFPLKKFGS
                     QGQQKSFVIAIKLAQFDYTRDVKGFKPV"
BASE COUNT          949 a          819 c          818 g          868 t
ORIGIN      
        1 agagtgaaaa tccggaaaaa tatttcgacc aggtcatcga aataaatctg accgaactgg
       61 aacctcacat caatggacct tttacgcccg atttagccac tcccgtctca aaatttgccc
      121 gggaagtgga aaagaacggc tggccggaaa aactggaggt aggcctgatc ggttcctgca
      181 ccaattcgtc gtacgaggac attacgcggg cagcctccgt tgcccggcag gctatcgata
      241 aaaagctcaa ggcaaaatcg gaattcacta ttacgccggg ttccgaacaa gtccgtttta
      301 ctgctgaaag agacggttat ctcgatatct ttgagaaaat aggaggggta gtgctggcca
      361 atgcctgcgg accctgcatc ggtcaatggg cgcgccataa tgcccataag caggaaaaaa
      421 attccatcat gacctctttt aaccgcaatt tcgccaagcg gaacgacgga aatcccaata
      481 cgcacggctt tgttgcatca cccgaaatcg tcaccgcatt cgccattgcc gggaccttaa
      541 agttcaatcc tgttaccgac aaactcctca atgaagaagg aaaagaggtc agcctggatg
      601 agcccgtcgg tatcgacttt ccggtcaacg gttttgcggt ggaagataac ggctatcagc
      661 ctccggcaga agacggaagc gctgtgaatg tgatcgtcga tcccaaatcc gaccggcttc
      721 agttactcgc tccgttccct gcctggaatg gtgaggacct gacagggctg caccttctta
      781 ttaaagcacg gggcaaatgc acgaccgacc acatcagtat ggccggtccc tggctcaaat
      841 accgcggtca tctcgacaat atctccaaca acctgctgat cggcgcaatt aatgatttca
      901 acggaaagac caacctggtg aaaaaccaga tgaccggtga atacggtgaa gtgcctgccg
      961 ttgcccggtt ttacaaagca aacggaacgg gctcaatcgt tgtgggcgat gaaaactatg
     1021 gtgaaggctc atcgcgtgaa catgccgcta tggagccccg ccatctgaat gtgagggcaa
     1081 tcctggtccg ttcgtttgct cgtatccacg agactaatct caaaaagcag gggatgcttg
     1141 ctttgacatt tgctaatcct tctgattatg ataagattaa agaagatgac cggatggata
     1201 tccttgggct gaaagagatg gctccgggca aacctctttc gattgttctc cggcacgccg
     1261 atggttcggt cgatcaattc ccggcgaacc acacgtataa taaaaatcag attgaatggt
     1321 tcaaggctgg tgctgcgctt aaccttttca gggaatagtg aatagtgagt tggggataat
     1381 tggttttgcg aggatataat atttttaagt aattaccgtt tatggattgg aaccaaaaac
     1441 tgatctcatt gagaacttta atccattatt tcatcctggt ccttccgctg gtttttattc
     1501 tggaacaagc cggcggccag gtgggaggca aaggcaccta ccagttcctg aacctgccga
     1561 actcagccag aattgccgct cttggaggca acttcctatc cattaacgat gatgatatta
     1621 ccttgaccct ggcaaaccct tcattgatca atgaagatat gcataaccag ctagggttca
     1681 gttttgtcga ttattataca gatgtgaatt acggttttgt gcaatacggg aggacattta
     1741 ataaagctgg gagttttgtc ggcaccatgc agtatatcga ttacggcaag ttccaggaag
     1801 ccgatgaagc cggaaatcaa tacggtacct ttcatgcggg agaatacgca ctcaatatcg
     1861 gatggggaag gaaactgacc ccgaggtggg caatcggcgc taattttaag atgatctact
     1921 ccggactggc ttcgtatcat tcttttgggt tgggagttga cgtggcaggc tcttatttca
     1981 gcaaagatga actctttgat gcttcattgc tgttcagaaa tatcggttac caggttgtgg
     2041 cttaccagaa cggtaaccat gagcctttgc ctttcgagat ccaggccggt atttcaaaga
     2101 aattcgaaca catccctttt cgtatcattg ccctggtcac acaccttgaa aaatgggatg
     2161 tccgctacga aagcagtgtc aatcccaaag gaggtctcga cccggtcacg ggcgaaccga
     2221 ataagatcag cggtgtggat gaatttgccg ataacctgat gcggcatgta atcttcggag
     2281 gggaagtcac catagcgaaa accgtcagcc tgcaaggatc ttataactac cagagacgac
     2341 aggagatgaa gatccccgga aaactttcca ccgttggctt ctcgtggggc atcgggatcc
     2401 gggtgaaaca attccagttc aattatgccc gttcggctta tcacctctcc gggtcaccta
     2461 attttattac cgttatgatt gatatggacg gcttccctaa aaagcaccag ccacaatcga
     2521 attaagatta tttttgcgga atgtacctgc aaaaattatc tgtaaccaac tttaaaaatt
     2581 acagcgaggc cacgctgcat ttttcagata agatcaattg ttttattggt gataatggcg
     2641 caggtaaaac gaacctgctc gacgctattc attacctctc cttttgcaag agctatttca
     2701 acccgatcga tacccagaac atcaggcatc atgaagattt tttcagcctg cacggaactt
     2761 acctgaagaa tggcgacagt cccgaccttg tttcctgtat ccaaaggtcc gagcaacgga
     2821 aagtttttaa gatcaatcaa aaggagtatg aacggcttgc cgaccacatc ggattgttcc
     2881 cactggtcat ggtttctcct tatgatcgtg atctgatcaa tgaaggcagt gaggttagaa
     2941 ggcggtacct cgacggagtg atctcccagt tcgacaaaca gtacctggac gatctaatcc
     3001 gctacaataa agctcttttg agccgcaata gccttctgaa acaagcatcg gaagggaagg
     3061 cccttaatca ttccctcatc gaattactgg acatgcaact ggccgattat ggccagaaaa
     3121 tacacgaaaa gagagccgga ttcctggaag cattctccac ggtatttcaa aagtattttg
     3181 cctttatttc cgaaggtaaa gaatcggtga cattgcatta ttcttcccag ctgttcagcc
     3241 ggagcctggg tgagctcctt gccgtttcgc tcgataaaga ccgttctgca aggtttacca
     3301 cacaaggaat tcataaagac gacctggaat tcagaatttc cggatttccg ctgaagaaat
     3361 tcggttcaca gggacagcag aaatcatttg tcattgccat aaaactcgcc caattcgact
     3421 atacacgcga tgtgaaagga tttaaacctg tttt
//
LOCUS       DAOQXS010000340         3429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_28817_length_3429_cov_3.689389, whole genome shotgun sequence.
ACCESSION   DAOQXS010000340 DAOQXS010000000
VERSION     DAOQXS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28817_length_3429_cov_3.689389"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..703)
                     /locus_tag="PKM34_09320"
     CDS             complement(53..703)
                     /locus_tag="PKM34_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNQ83830.1"
                     /translation="MPQQKKESKTLARMIGSLSGNPTEIKKESLRLLASYREKYGDKL
                     TEEEIRRKAANKIISNYSYYSAFTGGATSLTGIVPGIGTAIAALGGVSADVVLSMKFQ
                     IDMVMAIAAVYGHDIEEEYVQQTSFLVAGIGKLNFAVDEGKRIGAKAFANIMNQYLKK
                     GSQQAAKEITKKLGIQVSGKALQKAIPFGVGVIIGFTANKTLTWYIGRKARDYFAG"
     gene            807..2912
                     /locus_tag="PKM34_09325"
     CDS             807..2912
                     /locus_tag="PKM34_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNQ83831.1"
                     /translation="MKFRIFSLSIMMVFAVVTVSSKPVNIQTARITAGNFLRQTERLY
                     GYSPAGSYVIDDKPFVQRNDGLPVFYLFNLDPGFILISGDDAYTPVIGYSFESGLDLE
                     NAPAHVKGFIDNYAAQIAFIRDRQIPPSEEIQNEWADLLSGTTGEGAGLRTSREVEPL
                     LFCKWDQGAPYNMLCPEDEAGPGGHVWVGCVATAMAQVMYYWRYPAAGTGSHCYWPGN
                     WQYGQQCADFGNTYYNWEGMINSIDLVQVYPNAELQYHCAVSVDMDFSPNGSGSYSHM
                     VPERLAAFWGYSDAQILWKDDYSHSEWVEILQEELDASHPMYYSGYSNEGGHAFVCDG
                     YQGEYFHFNFGWGGSANGYFTLDNVGGFSYGQACIRYFVPSDPAYPYYIDGDHIMTSH
                     SGSFTDGSGPVGNYTVNQNATWLIDLGDSGDSIAGISLSFSMFDLGAGDTVTIYNGTT
                     RTDPILGAYTGTSVPQQINSTGDAMLVAFSSDSQVTGGGFYAEYYANSAVFCAGMTTM
                     TDMEGSMNDGSGSYNYHSNSYCTWYIRPEEAGTITLTFHSFDTESGVDKVTVFDGNVK
                     VGEFSGQEVPEPVVAQNGSIFMTWLTDGDNTGQGWSASYEAEAVGITEDPSASGINIY
                     PNPASGKINIEIKADREEEMEIRLVTLLGVDKYKGMISVRPGTNRITISVEDIPAGQY
                     FVELGSPLQKTVRQIAVIH"
     gene            3199..>3429
                     /locus_tag="PKM34_09330"
     CDS             3199..>3429
                     /locus_tag="PKM34_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83832.1"
                     /translation="MKRVILFLTGILLLQLSLVAAVNSGNNEEKAANDNTIRVIVSPE
                     MRELAGSLAHDFTRMQGGADVEILPVENGDFQI"
BASE COUNT          836 a          783 c          895 g          915 t
ORIGIN      
        1 gtaagcaatt tcatcatgaa attgaaatgc aaaatgcaaa tggaacatga aattacccgg
       61 caaaatagtc ccttgctttc cggccgatat accaggtgag tgttttgtta gccgtgaacc
      121 cgataatgac gccgacgcca aacggaatgg ctttttgcag ggctttcccc gaaacctgta
      181 tccccagttt tttcgtgatc tctttggcgg cctgctgcga ccctttcttc aggtattggt
      241 tcatgatatt ggcgaatgct ttagctccga tccgcttgcc ttcatccacc gcaaaattca
      301 gctttccgat tccagctacc aggaaactgg tctgttgtac gtattcttct tcaatatcat
      361 ggccataaac cgccgcaatg gccatgacca tgtcgatctg gaacttcatt gaaaggacga
      421 cgtcggctga aacgccaccc agggctgcaa tcgctgttcc tattccggga acgatgccgg
      481 tgagagaggt ggccccgccg gtgaaggcgg agtagtaaga gtagttggaa atgatcttgt
      541 tcgccgcttt tcggcggatc tcttcctccg tcagcttatc accgtatttt tcacggtaag
      601 aggccaatag tctcaggctt tctttcttta tttccgtggg attgcccgac agggaaccga
      661 tcatcctggc cagcgtttta ctctctttct tttgttgcgg cattgtgtga caattcagat
      721 agctaatttc gctataattg gaatatcagg aagaaatgat ttttaagctt taaaagttct
      781 atttttacaa acccaaataa tgcgcaatga aattcaggat tttctccctc tccattatga
      841 tggtgtttgc cgtcgttacc gtttcttcaa agccggttaa tattcagacg gcccggatca
      901 ctgccggaaa tttcctccgg cagacggaaa ggctctatgg ctatagcccg gccggatcct
      961 atgtgatcga tgacaagcca tttgtacaac gaaatgacgg attgccggtt ttctacctgt
     1021 tcaacctgga tccgggattt atcctcattt cgggcgatga cgcctatacg ccggtgatcg
     1081 gctattcttt cgaaagcggc ctggacctgg agaatgctcc ggcccatgtg aaagggttca
     1141 tcgataacta tgccgcccag atcgccttta tccgcgaccg gcagataccg ccatccgaag
     1201 agattcagaa tgaatgggcg gatttgttgt caggtacaac cggagaaggc gccggtttac
     1261 ggacaagccg ggaggtggag ccgctgcttt tctgcaagtg ggaccaggga gctccttaca
     1321 atatgctttg cccggaagat gaggccggcc cgggcggaca cgtgtgggtg gggtgtgtag
     1381 ctaccgccat ggcccaggtg atgtactact ggcgctaccc tgcagcaggc acgggaagcc
     1441 attgctactg gcccgggaac tggcagtacg gccagcagtg cgccgatttc gggaatacgt
     1501 attataattg ggaaggaatg atcaacagca tcgacctggt gcaggtctat ccgaatgccg
     1561 agcttcaata ccactgcgcg gtgtcggttg atatggactt cagtcccaac ggttcgggtt
     1621 cctattcgca tatggttccg gagcgtctcg ctgcgttctg gggatatagt gatgcacaaa
     1681 tcctgtggaa agacgactat tcgcattcgg agtgggtcga gatcctgcag gaggagctcg
     1741 atgcgagcca tccgatgtac tattccggct acagtaatga aggcggccat gcgttcgtgt
     1801 gcgatggtta ccagggcgaa tattttcact tcaatttcgg ctggggcggc agtgcaaatg
     1861 gctatttcac gctggataat gtcgggggat tttcttacgg gcaggcttgt atccgctatt
     1921 tcgtcccttc cgatccggct tatccttatt atattgacgg cgaccatatc atgaccagtc
     1981 acagcggttc ttttaccgac ggaagcgggc cggtgggaaa ttatactgtc aaccagaatg
     2041 ccacctggct gatcgacctg ggagactcag gtgattcaat tgccggtatt tcgctctcat
     2101 tcagcatgtt cgatcttgga gcaggagaca cggttacgat atataacgga acgacaagga
     2161 ccgatcccat ccttggggcc tataccggga cgtcggttcc acagcagatc aattctacgg
     2221 gtgatgccat gctggtggct ttctcatcgg atagccaggt taccggcgga gggttttatg
     2281 ccgagtatta tgccaacagc gctgtttttt gtgcggggat gacaacgatg accgatatgg
     2341 aaggatctat gaatgacggc agcgggagtt acaattacca tagcaactct tattgtacct
     2401 ggtatatccg tcccgaagag gccggaacca taaccctgac ttttcattcg ttcgataccg
     2461 aaagcggagt ggataaggtc accgtttttg acggaaatgt taaagtcggt gagttttctg
     2521 gccaggaagt cccggagccg gtcgttgcgc agaacggaag catttttatg acctggctga
     2581 ccgatggcga caatacaggc caggggtgga gtgcaagcta cgaggctgag gctgttggca
     2641 ttacggagga tccaagtgcc agcggcataa atatttatcc taatcctgca tccggcaaga
     2701 taaatattga aataaaagca gaccgggaag aggaaatgga gatccggctg gtgactttat
     2761 tgggcgttga taaatataaa ggtatgatct cggtcaggcc gggaaccaac cggataacga
     2821 tttccgttga agacattcct gccgggcaat actttgtgga actcgggtcg cctcttcaga
     2881 agacggtgcg gcaaattgct gtcattcatt aacttatatt ccggttgttg attttttcaa
     2941 cggctgttga atttctttac atcctatcct aataacttta tattcataag attacaactg
     3001 atttgtaatt gtgttgttta aatattcaac actacagact ggtttttatc cgcaactttt
     3061 cccaaggtga attcttaaga gatagtaaat caatgttata gaattactgg catcaaaatt
     3121 gaccggctca gtttcaaaac aggtaaaatg aaatccggaa gcaatcaatt ttctaaaaac
     3181 attttaatca tcacgattat gaaaagggtg atcttatttt taacgggaat tctgttactc
     3241 cagttatccc tggtggcagc ggttaatagc gggaacaacg aagaaaaggc agcaaatgat
     3301 aataccatcc gggtgatcgt atccccggaa atgagggaat tggccggcag tttggcccat
     3361 gactttaccc ggatgcaggg gggagcggat gtggagattc tgccggtgga gaatggagat
     3421 tttcagata
//
LOCUS       DAOQXS010000341         3429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_28826_length_3429_cov_3.080913, whole genome shotgun sequence.
ACCESSION   DAOQXS010000341 DAOQXS010000000
VERSION     DAOQXS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28826_length_3429_cov_3.080913"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PKM34_09335"
     CDS             <1..1061
                     /locus_tag="PKM34_09335"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNQ83833.1"
                     /translation="QISAVLGPCAGGAVYSPAMTDYIIMAKDSSYMFVTGPEVIKAVT
                     HEEVTMEDLGGAMTHNAKSGVAHLIAENDEQALMMIRELLGYLPSNNMEDPPVESCTD
                     SITREDLSLQTLIPEDPNKPYDIKELITTVVDNHNFFEVQPHYAPNIVVGFGRLDGRS
                     VGIVANNPAHLAGVLDISSSVKGARFVRFCDAFNIPIITFVDVPGFLPGTNQEFGGII
                     KHGAKLLYAYCEATVPKITVITRKAYGGAYDVMSSKHIGADVNFAYPTAEIAVMGPEG
                     AVNILYKKENLNEEGRKAKVEEYKTTFASPYKAAELGYIDEIIYPKQTRFKLIQSLEM
                     TQNKSKSNPPRKHGNIPL"
     gene            1225..1560
                     /locus_tag="PKM34_09340"
     CDS             1225..1560
                     /locus_tag="PKM34_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HNQ83834.1"
                     /translation="MDSTRQHKMARLLQKEMGDIFEIDMRRLFPGAMITITKVNVTKD
                     LSVARFNLSIFALKDKKPTVEEIRAHIPEFRYKLGNKIRKQVRIIPQVEFYEDDSLDY
                     IENLEKLMK"
     gene            complement(1581..>3429)
                     /locus_tag="PKM34_09345"
     CDS             complement(1581..>3429)
                     /locus_tag="PKM34_09345"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNQ83835.1"
                     /translation="NPQRIDFIFFNEQFRTRSYEARKITYNGMFVSDHWPVLAQLECS
                     EKGARPAERSGEGPAGRGQGDTTIVQTFTFDSIVTRRAEFQFPGKEKRYEKILMYYTL
                     KCDSLTPHDQYPCGEWDYTTYVKVFVPTGRYDSLFYTRTDSLGNVVRSWFGESIEVRE
                     PFEIGRFITPYGKRLDLGLNGFTWIYDVTDYEPLLHGTVDLKAGNGQELLDLRFLFIE
                     GIPPRDVVSVRNIWPEGSYTYKELADDAKLQTVRQKLADNASTFSIRARISGHGHFGP
                     FNCCEWDPKTHTYLGNGENRFDWKVWRDCGMNPVHPQGGTWQFDRAGWCPGTFVDTYD
                     FEITPFVEPGEEVSIDYLIQPYDTANGEEGGNFEMAMHLIEYSSPNYKLDLELTDILA
                     PGNRHEYRRMNPVSLNPVIRIRSLGTDTVRNITFFYGLEKGEKITYDWQGKLGFMQSA
                     DIILPKPSWQGADDKSMFIAEIITVNGLQDERESNNTQVSAIVKPVILPDKFIIHVKT
                     QGFGRAADNAWKIKDETGRVVYERLDFLDDTVYDDPVSLPPGAYDFTFTDKNEDGLIR
                     HWWLHWEDPGKVGQNGELKILGSDEKEIMNLGYDFAEKRTLQFFVGEPE"
BASE COUNT          846 a          882 c          866 g          835 t
ORIGIN      
        1 cgcagatttc ggccgtactt ggtccgtgcg cggggggagc tgtttattct ccggccatga
       61 ccgattatat catcatggcg aaggactcgt cgtatatgtt tgtgacaggc ccggaagtga
      121 tcaaggcagt gacacatgaa gaggtgacga tggaagacct tggcggggcg atgacgcaca
      181 atgcgaaaag cggtgtggcc catctgatcg cggaaaatga tgaacaggcg ctgatgatga
      241 tcagggaact gctgggttac ctgccgtcga acaacatgga agacccgccg gtagagagct
      301 gcaccgacag cattacacgg gaggacctga gcctgcaaac cctgatccct gaagatccca
      361 acaaacccta cgatattaag gagctgatca caacagtcgt cgataatcat aatttctttg
      421 aagtccagcc tcattatgcg ccgaacatcg tagtcggttt tggccggctc gacggccgct
      481 ctgttggcat tgtggcaaat aaccccgctc acctggccgg tgtgctcgac atcagttctt
      541 cggtcaaagg agcacggttt gtcaggttct gcgacgcttt caatatcccg atcatcacgt
      601 tcgtggatgt tccgggcttt ttaccgggga ccaaccagga attcggcggg atcatcaagc
      661 acggggcgaa attactctac gcttattgcg aagccacggt gccgaagatc acggtcatca
      721 cccggaaagc ttacggcggt gcttacgacg ttatgtcgag caagcatatc ggagccgatg
      781 tcaactttgc ttacccgacg gcagagattg ccgtcatggg cccggaagga gcggtgaaca
      841 tcctctataa aaaggaaaat ctcaatgaag aagggcgaaa agccaaggtg gaggaatata
      901 agaccacttt tgccagtcct tacaaagcgg ctgaactggg atacatcgac gagatcattt
      961 acccgaaaca gacccgtttc aagctgatcc agtcactgga gatgacacaa aataaatcga
     1021 aatcgaatcc gccgaggaaa cacggaaaca taccacttta aaaattccag atccccagca
     1081 ccaaatccca aacaaatttc aaaaataaag accaagaata ttttaaatgt taatcttgta
     1141 gttttggatt tgtttggttc ctgggatttg gtgcctggaa tgtaatgaat tgtatttgtt
     1201 agaagaagaa ttattattac ttgaatggac tcgacaagac aacataagat ggcccggctg
     1261 ctgcaaaagg agatgggcga tattttcgag atcgacatgc gcaggctgtt cccgggcgct
     1321 atgatcacga taaccaaggt aaacgtgaca aaagaccttt cggtggcccg gtttaacctg
     1381 agcatctttg ctttgaaaga caagaaaccc acggttgagg agatcagggc gcacattccc
     1441 gaattccgat acaagctggg caataagatc cgcaaacagg tcaggataat tccccaggtt
     1501 gaattttatg aggatgattc actcgattat atcgagaacc tggagaaatt gatgaagtga
     1561 ttttgaaaga aatcatcaat ttattcgggt tccccgacga aaaactgcag tgtcctcttc
     1621 tccgcaaaat cgtaccccag gttcatgatc tccttttcat ccgaacccag gatctttaat
     1681 tccccgttct ggcctacttt cccgggatcc tcccagtgga gccaccagtg gcggatcagc
     1741 ccgtcctcgt tcttgtcggt gaaagtgaag tcgtaagcgc cgggtggcaa gctgacagga
     1801 tcgtcataaa ccgtatcgtc gagaaaatcc agcctttcgt aaacgaccct gccggtttcg
     1861 tctttaattt tccacgcgtt gtcggcggcc ctcccgaagc cctgcgtttt gacatggatg
     1921 ataaatttat ccgggaggat aaccggtttg acgatcgccg aaacctgggt gttattgctt
     1981 tccctctcat cctgcaagcc attgaccgta attatttcag cgatgaacat tgatttatca
     2041 tccgctccct gccaggatgg cttaggcagg ataatatcgg ccgattgcat gaaaccaagt
     2101 ttgccctgcc agtcgtaggt gatcttttcc cctttctcca gcccgtaaaa aaaggtgata
     2161 tttctgacag tgtcggtgcc gaggctcctg atcctgatca ccgggttcag gctgacagga
     2221 ttcatccttc ggtactcatg acggtttccg ggcgcaagga tgtcggtcag ttccaggtcg
     2281 agtttataat ttggtgagga atattcgatg aggtgcatcg ccatttcgaa attgcctccc
     2341 tcttcgccgt tggccgtatc gtacggctgt atcaggtaat caatcgatac ctcttcgccg
     2401 ggttcgacga atggggttat ttcaaagtca taggtgtcga cgaatgtccc ggggcaccag
     2461 ccggcacggt cgaactgcca ggtaccgccc tgcggatgga ccgggttcat cccgcaatcg
     2521 cgccatacct tccagtcgaa gcggttctcc ccgtttccca ggtacgtatg tgtcttcgga
     2581 tcccactcgc agcagttgaa tggcccgaaa tggccgtgcc cggaaatccg ggccctgatg
     2641 ctgaaggtgc tggcattatc cgccagtttt tgcctgacag tctgcagctt tgcatcatcc
     2701 gctaattctt tataggtata agaaccttcg ggccagatat tcctgaccga aaccacatcc
     2761 cgcggcggaa tcccttcgat gaaaaggaac cgcaggtcga gcagttcctg gccgttgcct
     2821 gccttcagat caaccgttcc gtgtagcaac ggctcataat cggtcacatc ataaatccag
     2881 gtaaatccgt tcaggcccag gtcgagccgc tttccgtaag gtgtgatgaa ccggccgatc
     2941 tcaaaaggct ccctgacttc aatggattca ccgaaccacg aacggaccac atttccgagc
     3001 gaatcggtcc gggtgtagaa caaggaatcg taacggcctg tcgggacgaa tactttgacg
     3061 tatgtcgtat aatcccattc cccgcagggg tattggtcgt gcggtgtcag cgaatcacac
     3121 ttcagcgtgt aatacatcag gatcttttca taacgctttt ccttgccggg aaactgaaac
     3181 tcagctcttc tcgtcactat ggaatcgaaa gtgaacgtct gaacgattgt cgtatccccc
     3241 tgacctctgc ctgcgggtcc ttccccgctg cgctcggcag ggcgggctcc cttttcgctg
     3301 cattccagct gagccagcac cggccagtgg tcggaaacaa acatgccgtt ataggtgatc
     3361 ttcctggctt cgtacgacct ggtcctgaat tgttcattaa agaaaataaa atcgatccgt
     3421 tgcgggttc
//
LOCUS       DAOQXS010000342         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_28898_length_3424_cov_4.064114, whole genome shotgun sequence.
ACCESSION   DAOQXS010000342 DAOQXS010000000
VERSION     DAOQXS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28898_length_3424_cov_4.064114"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKM34_09350"
     CDS             complement(<1..413)
                     /locus_tag="PKM34_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNQ83836.1"
                     /translation="MNYLRQYIIPFSGLKAGNYDYSFEIDGRFFEEFEYSEIREGKLH
                     VDCLLEKQDRMMIFSFRIKGAVRVICDRCAGEFDWPVEGKQQLIVKFGQEHTEEAEDI
                     LVITEKEHELDLSQYLYEYIHLLVPIRKIHGTDENG"
     gene            596..1507
                     /locus_tag="PKM34_09355"
     CDS             596..1507
                     /locus_tag="PKM34_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNQ83837.1"
                     /translation="MFNRMIAGLLPYMPKSFVWIFSKRYIAGKKIEDAIRVSRELNGQ
                     GIKVTVDLLGEFITRLSEAEKNKIEYLDIIDTFTKENIDGNFSLKPTMFGLLIDPRVC
                     YENIREIVARAASYNNFIRIDMEDSQCVDLEIDLYRKLKAEFPKNVGLVVQAYLKRTM
                     SDLENLMDIHSKEIPLNYRLCKGIYVEPAEIAYKKYDEINSHFLEDLEFMLKNGVYPG
                     IATHDKPLVDGAYKLIEKYNVPKNMYEFQMLYGVTPELRKSIVDKGHTMRVYVPFGEQ
                     WFAYSTRRLKENPKMAWLIIKAIFVRG"
     gene            1611..3119
                     /gene="atpD"
                     /locus_tag="PKM34_09360"
     CDS             1611..3119
                     /gene="atpD"
                     /locus_tag="PKM34_09360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNQ83838.1"
                     /translation="MENNMTGKIAQIIGPVVDVSFEDEAHLPNIYEALEVTNDKGEVV
                     VLECQQDIGENTVRTVAMDSTDGMRRGMEVVRTDKPISMPATEEIRGRLFNVIGKPID
                     GLGEVKGSKQYPIHREPPTFENLTTRQEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVIIMELINNIAKVYSGMSVFGGVGERTREGNDLLREMIESGVIQYGDEFLKDMEKG
                     GWDLSKVDREKLKDSNITLVFGQMNEPPGARARVALSGLTLAEYFRDGDEKSGGRDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKRGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLNRKIFEIGIYPAVDPLDSTSRILSPEIVGEDHY
                     RTAQRVKQILQRYKELQDIIAILGMDELSDEDKLTVSRARRVQRFLSQPFHVAEQFTN
                     IPGTIVPIEDTIKGFKMIMDGEVDEYPEAAFNLVGTIEEAIEKGKKMLAENE"
     gene            3121..3357
                     /gene="atpC"
                     /locus_tag="PKM34_09365"
     CDS             3121..3357
                     /gene="atpC"
                     /locus_tag="PKM34_09365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNQ83839.1"
                     /translation="MKLEIITPEQTLFTGEATLVQMPGIDGSFEILSNHAPMISVLSA
                     GRIKIQDAQKQLQYFDVKGGVVEVLNNKVLVLAE"
BASE COUNT          938 a          850 c          825 g          811 t
ORIGIN      
        1 ccgttttcat ccgtgccgtg aatctttctt atcggaacca gcaaatgaat atattcgtac
       61 aggtactggc tcagatccag ctcatgctcc ttttcggtga tgaccagaat gtcttctgcc
      121 tcttccgtat gctcctgccc gaatttcacg atcagttgct gcttgccctc cacaggccag
      181 tcaaattccc ccgcacaacg gtcgcaaatt actcttactg cccctttaat cctgaaacta
      241 aagatcatca tcctgtcctg cttttcaagc agacaatcta catgtaattt cccttccctg
      301 atttccgagt attcaaactc ttcaaagaac ctgccgtcaa tttcaaagga atagtcgtaa
      361 tttcccgcct ttaatccgct gaaagggatg atatattgcc tcaggtaatt cacgtatatc
      421 cctccttaaa aattggggtg caaatttagg gatattattt tgaataacaa aagaataatt
      481 tgaaatccaa aatttgcggc acttttgtta aagaattaac aaaagattaa ttttgcaccg
      541 tttcaatttc gaaatcgtgc atttaaagtg ttttaaatta taaatttcta aagagatgtt
      601 taacaggatg atcgccggcc tgctgccgta tatgccaaaa agctttgtct ggatcttttc
      661 caaaagatat attgccggca aaaaaattga agatgcaatc cgcgtttcgc gggaactgaa
      721 cggccaggga attaaagtca cggtcgacct cctcggggag ttcatcaccc ggctcagtga
      781 agccgaaaag aataaaatag agtacctgga tattatcgat acgtttacga aggaaaatat
      841 tgacggtaat ttttccttga aaccgaccat gttcggatta ctgatcgatc cccgggtttg
      901 ctacgaaaat atccgggaaa tagtggccag ggctgcatct tacaataatt ttatccggat
      961 cgacatggag gactcacagt gtgtcgacct ggagatagac ctttaccgga agctgaaagc
     1021 cgaatttccc aagaatgtcg gcctggtggt acaggcttac ctgaagcgga cgatgagcga
     1081 tcttgaaaac ctgatggata tccactcgaa agagatcccg ctgaattaca ggctctgtaa
     1141 ggggatttac gttgaaccgg cagagattgc ctacaagaag tatgacgaaa tcaacagcca
     1201 tttcctggaa gacctggaat ttatgctgaa aaatggcgtt taccccggca ttgccactca
     1261 cgataaaccg cttgtggacg gcgcatacaa gctcatcgaa aaatataatg tacccaaaaa
     1321 catgtatgag ttccagatgc tatacggcgt cacaccggag ctgaggaaat ccatcgttga
     1381 caaaggacac acgatgcggg tttatgttcc gttcggcgaa caatggtttg cttacagcac
     1441 ccgccgcctg aaggaaaatc cgaaaatggc ctggctgatc atcaaggcga tttttgtgag
     1501 aggataacga taatgtagag acgcaacatg tgttatgtcg taaagacgca aaatattgcg
     1561 tctttacgat aatgtattat ctttgccacg aaaaatcaca aagctatccc atggaaaaca
     1621 atatgaccgg taagatcgcc cagattatcg ggccggttgt cgacgtttct tttgaagatg
     1681 aagctcatct tccgaatatt tacgaagccc ttgaagtaac caacgataag ggtgaagtgg
     1741 tggtgctgga atgccagcag gacatcggtg aaaacaccgt ccggaccgtt gcgatggatt
     1801 ccaccgacgg gatgcgccgc ggaatggaag tcgtcaggac cgataaaccg atctccatgc
     1861 cggcaacgga agagatccgc ggccgtttgt tcaacgtgat cggcaaaccg atcgatggcc
     1921 tcggagaggt caaaggatca aagcagtacc cgatccaccg cgaaccgcct acctttgaga
     1981 accttaccac ccgccaggaa gtactttata ccggcataaa ggtgatcgac ctgatcgagc
     2041 cctactccaa aggaggcaaa atcgggttgt tcggtggcgc cggtgtgggc aagaccgtca
     2101 tcatcatgga gctcatcaac aacatcgcga aagtttattc cggtatgtcg gtgttcggcg
     2161 gtgtcggcga acgtacgcgc gagggcaacg acctgcttcg cgaaatgatc gagtcgggcg
     2221 ttatccagta tggggatgaa tttttgaaag atatggaaaa aggtggatgg gacctttcga
     2281 aagtcgaccg cgaaaagctg aaggattcca acatcaccct ggtattcgga cagatgaacg
     2341 aacctcccgg agcccgcgcc cgcgtggcat tatcggggct cacgctggcg gagtatttcc
     2401 gcgatggcga cgaaaaatcg ggcggccgcg atatcctgtt ctttatcgac aatattttcc
     2461 gtttcaccca ggccggatcc gaagtgtcgg ccctccttgg ccgaatgcct tcggcagtgg
     2521 gttaccagcc gacgctggct accgaaatgg gtatcatgca ggaacgcatc acctccacca
     2581 agcgcggttc catcacctcc gtacaggcgg tctacgtacc agccgacgac cttaccgatc
     2641 ccgcaccggc cacgactttt gcccacctgg atgccactac cgtactcaac cgtaagattt
     2701 ttgagatcgg tatctacccg gctgtcgacc cgctggattc aacttcccgc atcctcagcc
     2761 ctgaaatcgt cggcgaagac cattaccgga ccgcacagcg cgtgaagcag attctgcagc
     2821 gctataaaga attgcaggat atcatcgcca tcctcggaat ggacgagctc tcggatgaag
     2881 acaagctcac ggtgagccgt gcccgccgtg tccagcgctt cttgtcgcag ccgttccatg
     2941 tagcagaaca gtttaccaat attcccggca ccatcgtccc gatcgaagat accatcaaag
     3001 gattcaagat gatcatggat ggcgaggtcg acgaataccc ggaggcagct ttcaatctcg
     3061 taggcaccat cgaagaggct atcgagaaag ggaagaaaat gctggcagag aacgaatgat
     3121 atgaaactgg aaatcataac ccccgaacag acgctattca ccggtgaagc caccctggtt
     3181 cagatgccgg gcatcgacgg ctctttcgag atcctgagca accatgcacc gatgatctcc
     3241 gtgctgtcgg ccggccgaat caagatccag gacgcccaga aacagcttca gtatttcgac
     3301 gtaaaaggcg gagtggtaga ggtgctgaac aacaaagtgc tggttttagc cgaatagttg
     3361 ttgtaacccg tagaactaca ggtagttata aatctttttt ataactcttt gagatggcat
     3421 ctca
//
LOCUS       DAOQXS010000343         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29064_length_3410_cov_4.014009, whole genome shotgun sequence.
ACCESSION   DAOQXS010000343 DAOQXS010000000
VERSION     DAOQXS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29064_length_3410_cov_4.014009"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1496)
                     /locus_tag="PKM34_09370"
     CDS             complement(225..1496)
                     /locus_tag="PKM34_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ83840.1"
                     /translation="MNDMFINNNGLPFCKGCSHTTIAKNTEKALEKIGLKPLDVILVT
                     DIGCHGIIDKSFNTHTVHGLHGRSVALGAGVSAGLRDAGKKVIVFLGDGGATIGMQHI
                     IDAAHNGFNMTVVIHNNMLYGMTGGQPSEYTPYGFKTPTKPGGSTKMSYDICKIVAAA
                     GAPYVRRILAIGDISDALAEAFGRPGFSLVEVMEICPSYGVKSNPEMKLAKVVENSGL
                     ATTVVADNPAEAYRTNLREGLPSLLDAVPIIPAKAGISLPKEMRIMFCGSAGEGVQVA
                     AELLAVAGIRAGLEATKKGSYPVTVGVGFSAAEVILSSGEINYTGTVTPDYMIVTSQE
                     GLDYGRKAAGRMKAGKIFLDASLEPPVTGAEIVSFPFREKVTARNASFMGMFYFVNSS
                     GVFPLDVMKEAFNANKIAKKVDAGQFLELLA"
     gene            complement(1489..2607)
                     /locus_tag="PKM34_09375"
     CDS             complement(1489..2607)
                     /locus_tag="PKM34_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ83841.1"
                     /translation="MKDKQTVFIDSSRLITESLVRAGADVFIGYPITPANLLYAYSER
                     RFPTFFAAPDEITTLQWMSGFATTGHLPVTATSFPGYALMIESINMAYMMELPMVIVL
                     IQRLGPATGTATCGAQGDLNVIYGTLSGGFPLPCIAVSNMHDCWDLPPKALQMAVDLR
                     TPVVLLTSKEEVMTYKSFDLSSLNEIEPVTRTPYSGKSPYMPYAPMENLVPEFLPVTE
                     TGHQVRYTASTHDKTGIIQNTTPDALANSTRLARKITTNLENYLFDDYDRQEGAKTLL
                     ITYGITTQAAADAVKAWRSEGKKASLLVMKTLSPITKRYFDICKEYDNIVIAEENLDG
                     ELRRIMFGQGDQARITGVNKIGQMIEPGEIIKALESYE"
     gene            2864..>3410
                     /locus_tag="PKM34_09380"
     CDS             2864..>3410
                     /locus_tag="PKM34_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ83842.1"
                     /translation="MSATLYNPPDHYLVDDLYTEEHKIVRQAARDWVNRAIKPVIEEY
                     YEKAIFPLHLIKEMGENGLFGPFIPEEYGGAGLDHISYGLIMQELERGDSGVRSMASV
                     QTSLVMYPIFSYGSEEQKRKYLPKLASGELIGCFGLTEPNHGSDPGSMITRIKDMGDH
                     YLLNGAKMWITNSTIADVFIVW"
BASE COUNT          824 a          896 c          854 g          836 t
ORIGIN      
        1 ttctgagcga agcccgggtt gaaggtaaga gtgagggcaa tgcataaagc cccggctgcg
       61 gcagccgatc gttttaaatt gattttcagc attttgataa gttattaata aaaaataaaa
      121 ttaccggatg gaatggtaac ccaaaagtag gaaatagacg gatataaaac aattatgggt
      181 ttataaaagg atgattcggg tttgcaacag ggttggaaac cctgtcaggc caacagctcc
      241 aaaaactgcc ccgcatcaac tttcttcgca atcttgttcg cattgaacgc ctctttcatc
      301 acatccagcg gaaaaacccc cgaagaattg acaaaataga acattcccat gaatgaagcg
      361 ttacgggccg tcaccttctc cctgaacggg aagctgacga tctccgcacc ggtgacaggc
      421 ggctccaggc tggcatccag gaagatcttg cccgccttca tcctccccgc tgctttacgt
      481 ccgtaatcca gcccctcctg tgaggtcacg atcatgtaat cgggtgtgac tgtccccgtg
      541 taattgatct ctcccgatga aaggatgact tctgccgctg aaaaaccaac gccgacagta
      601 accggataac ttcccttctt cgttgcttcc agccctgcac ggatgccggc aacggccagc
      661 aattccgccg ccacctgcac cccctcgcct gccgaaccgc agaacatgat gcgcatctcc
      721 tttggcaagg agatccccgc tttcgcgggg atgatgggga ccgcgtcgag caatgaaggc
      781 agtccttcgc gcagattggt acggtaggct tccgccgggt tgtcggcaac aacagttgtt
      841 gccaaaccgc tgttctccac caccttcgcc agtttcatct caggattgga tttcacgccg
      901 taactcgggc aaatctccat cacctccacc aatgaaaatc cgggccggcc gaacgcttcg
      961 gccagcgcat cggaaatatc gccgatggcc aggatgcgcc tgacataggg cgcaccggcc
     1021 gcagccacga tcttgcaaat atcatacgac atcttcgttg aaccgcccgg tttggtcgga
     1081 gtcttaaaac catatggagt gtactcgctg ggctgcccgc cggtcatgcc gtatagcata
     1141 ttgttgtgaa tgacaaccgt catgttgaag ccgttgtgcg ccgcatcgat aatatgttgc
     1201 atgccgatgg tggcgccgcc atcgcccagg aaaacgatca ctttctttcc cgcatcgcgg
     1261 aggccggccg atacaccggc gccaagagct accgagcgcc cgtgcaatcc gtgtaccgta
     1321 tgggtgttaa aggatttgtc gatgatcccg tggcagccga tgtcggtcac caggatcaca
     1381 tccaggggct tcaacccaat cttttccagc gccttttcgg tatttttggc aatagtcgta
     1441 tgactgcatc ctttgcaaaa agggagtccg ttgttattta taaacatatc attcataact
     1501 ctccaaagct ttaatgatct cacccggttc tatcatctgt ccgattttat taacaccagt
     1561 gatcctcgcc tgatccccct gcccgaacat gatcctgcgc aattccccgt cgaggttctc
     1621 ttcggcaatg acaatgttgt catattcttt acagatatca aaataccttt ttgtaatcgg
     1681 gctgagcgtc ttcataacaa gtaatgacgc tttctttccc tcacttcgcc aggctttgac
     1741 agcatcggct gctgcctggg tcgtaatccc gtaagtgata aggagcgttt ttgccccctc
     1801 ctgcctgtcg taatcatcaa acaggtaatt ctccaggtta gtggtaattt tacgcgccag
     1861 gcgggtggaa ttggccagtg catcgggcgt cgtattctgg atgatcccgg tcttatcgtg
     1921 agtggacgcc gtgtacctga cctggtgtcc ggtctccgta acggggagaa attcggggac
     1981 caggttctcc atcggggcgt agggcatata gggtgatttg ccggaatacg gggttcgggt
     2041 tacgggttct atttcattta atgatgacag gtcaaaactt ttataggtca tgacctcctc
     2101 ttttgatgtc agcagaacga ccggcgtacg aaggtcaacc gccatctgca gggcttttgg
     2161 cggaaggtcc cagcagtcgt gcatattgct cacggcgatg cagggcagcg ggaaaccgcc
     2221 cgagagggtt ccgtagatga cattaaggtc gccctgggcg ccgcaggtgg ctgtaccggt
     2281 ggccggcccc agcctctgga tcagcacgat caccatcggc aattccatca tgtaggccat
     2341 attgatcgat tcgatcatca gggcatagcc ggggaaagag gtagctgtaa ccgggagatg
     2401 gcccgtggtg gcaaaaccgc tcatccactg cagggtggtg atttcgtcgg gggcggcgaa
     2461 aaaagtagga aacctgcgct cgctgtaagc atagagcagg tttgcaggtg tgatcgggta
     2521 accgatgaac acatcggcgc cggccctcac cagcgactcg gtgatcagcc tggagctgtc
     2581 gataaatact gtttgtttat ctttcatctt ataaattttc agacaatcat tattaaacac
     2641 aaaggccacg aagggtttca caaaggaaca caaaggaatg atgcaggtat ttcgttactt
     2701 tgtgcgcctt tgtgatatac tttgcgttac tttgtggtta atctttttaa cacaacatca
     2761 taataaaaag cgcgtcaaag ttagaaatat tattttctgt taataaatta acaattaaaa
     2821 gaaatctttc tatttttgtc tccaaattca agaaacaaac actatgtcag ctactcttta
     2881 caatccgccc gatcattacc tggtagatga tctctatacc gaagaacaca agatagtccg
     2941 gcaggctgcc cgcgactggg tcaaccgtgc tatcaagcct gttattgaag aatattatga
     3001 aaaagccatt ttcccgttgc atctgatcaa agagatgggc gaaaacggtc ttttcggccc
     3061 gttcatcccg gaagaatacg gtggagccgg gcttgaccat atttcgtacg gactgatcat
     3121 gcaggaactg gaaaggggcg acagcggtgt acgttcgatg gcgtcggtac agacctccct
     3181 ggtgatgtat ccgatcttct cctacggttc ggaagagcag aagcgcaagt atttaccgaa
     3241 gcttgcctcc ggcgaactga tcggctgttt cggcctgacc gagccgaacc acggctccga
     3301 cccgggcagc atgatcaccc ggatcaagga catgggcgac cattacctgc tcaacggcgc
     3361 caagatgtgg atcaccaatt caaccatcgc cgatgtattc atcgtatggg
//
LOCUS       DAOQXS010000344         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29068_length_3410_cov_3.331148, whole genome shotgun sequence.
ACCESSION   DAOQXS010000344 DAOQXS010000000
VERSION     DAOQXS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29068_length_3410_cov_3.331148"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PKM34_09385"
     CDS             complement(<1..918)
                     /locus_tag="PKM34_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83843.1"
                     /translation="MTKKFTIRILLFLALLAGLNFVIDWAFKSFSAHRWVNLKMDEQF
                     GEYDGTLTWLAMGNSHNCVNTRILDNSFNYGSPGENYIRTYYKLKYILEKTGHKPEYL
                     LLQADISSFGPKISNRYEYNYYWIKYVDYLELARIKNDRDIINYWIEGKFFSYAGNYK
                     DFWLSIVYRIKMKEVNMFRGYREHRDYRNFADVPDRQKVAWDKAQMILTRNEALDPAI
                     RVYFDMIMQLCREHQVKVLLIRFPEAKEFYEEQERLVPVGKLYEEVTAIAEKYTVFNG
                     ILDYHDMFFEHPEYFFDPDHLNVKGSDLFT"
     gene            complement(925..2376)
                     /locus_tag="PKM34_09390"
     CDS             complement(925..2376)
                     /locus_tag="PKM34_09390"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNQ83844.1"
                     /translation="MLFNSVDYLLFLPLVTALYFVIPVKWRWAFLLLASYFFYMCWKA
                     EYAILILFTTLVDYFVALKMDSLSTRRQKKPYLILSLAANLGMLAGFKYLNFFSESVN
                     FAMAGLDLPGRVPVLDILLPVGISFYIFQSLSYTVDVYRGTTKAERHAGKFALYVSFF
                     PQLVAGPIERSNNLLPQVNHPQAFSQVRLVSGLKLIAWGFFKKVVIADRLGVFVSYVY
                     ENPDEHKGITILLATVLFAFQLYGDFSGYTDIARGSARILGYDLMINFNRPMIARSLR
                     DFWNRWHISLTTWFRDYLLYSIPYVKNKKVMVSLMYRNLVITYLLMGLWHGAAWTFVI
                     FGLFHGILLVLENITENYRLRFFRWTGISRFPGVQTTLGMVATFILLVFSLFFFRAGS
                     IENSFILISNAFDFSTFKQTIGYIIRNNELIFGILMVIILLTIEYFHEKYNLVKVVLS
                     RPTVIRWALYAGFVIFILFFGVLYKQKFIYFQF"
     gene            2467..>3410
                     /locus_tag="PKM34_09395"
     CDS             2467..>3410
                     /locus_tag="PKM34_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ83845.1"
                     /translation="MSKRSLLRDVTGVVATRIFLITAGLLSSIVLSRNLGPENFGIYS
                     AILVLPLVVVSFAQLGMRASSIFHIGRKQYEPGEIAGSVVLILVMTSMLGILITALGY
                     LIMDEESFTGLYIILVLLSLPFRLAMAYFGGIFLGREQISRSNFINWFAEFLNLLAVL
                     VFVWLLGWGIKGALISLLASQATISTWALFVIRRDIGIRLKFHPEVLKSLLSMGVLFA
                     TSFGIIQLNYRIDILLLQKLSTMEEVGFYSLGVSIAEKLWQLPLAIGVVLMSRTANAD
                     DQQMINRISARLVRVALLAVFLASVVLFVISPWIVPAI"
BASE COUNT          892 a          810 c          828 g          880 t
ORIGIN      
        1 ggtaaaaagg tccgatcctt taacattgag atggtccggg tcaaaaaagt actccggatg
       61 ttcaaaaaac atatcgtgat aatccagaat cccattgaaa accgtatact tttcagctat
      121 tgcggtaacc tcctcataca attttccgac cggtaccaat ctttcttgtt cttcgtaaaa
      181 ctcttttgct tcgggaaaac ggatcagcag cactttcacc tggtgctccc ggcacaactg
      241 catgatcata tcaaaatata cccggatggc cggatccagg gcctcatttc gggtcaggat
      301 catttgcgcc ttgtcccagg ctactttctg acgatcaggc acatcggcaa aattccggta
      361 atccctgtgt tcacggtatc cgcgaaacat gtttacctcc ttcattttga tccggtacac
      421 gatcgataac cagaaatcct tgtagttgcc ggcgtacgaa aaaaacttcc cttcaatcca
      481 gtaattgatg atatccctgt cgtttttgat ccttgccagt tcaaggtaat ccacatactt
      541 tatccagtag taattgtatt cataacggtt ggatattttt ggtccgaaac tggaaatatc
      601 ggcctggagc aaaagatatt ccggcttatg gcctgtcttt tcgaggatat atttcagctt
      661 gtaataagtc cggatgtaat tctcccccgg agaaccgtaa ttaaaactgt tgtcgaggat
      721 acgcgtgttg acgcagttat gcgaattccc catggcaagc cacgtcagtg ttccgtcgta
      781 ttccccgaac tgctcatcca tcttcagatt gacccatctg tgcgcggaaa acgatttgaa
      841 agcccaatcg atgacgaagt tcaggcctgc cagaagggcg aggaacaaca ggatccgtat
      901 cgtgaatttt ttcgtcatgc cttatcagaa ctggaaatag atgaattttt gtttgtaaag
      961 cacaccgaag aagaggatga aaatcacgaa tcctgcatat aatgcccaac gtatgactgt
     1021 aggcctggaa agcaccacct ttaccaggtt gtatttttca tgaaaatatt caatggtcaa
     1081 taagatgatt accatcaata tcccaaaaat caattcattg ttcctgatga tgtaaccgat
     1141 ggtttgtttg aaggtgctga agtcaaatgc atttgaaatc agtatgaagc tgttttcgat
     1201 gctgccggca cggaagaaaa acagggaaaa gacgagcagg atgaacgtag cgaccattcc
     1261 gagggttgtc tgcacccccg gaaaccggga aattcctgtc cagcggaaga acctcagccg
     1321 gtaattttcg gtaatattct ccagcaccag caggattccg tgaaacaggc cgaagatcac
     1381 gaaagtccat gcggcgccat gccataatcc cataagcagg taggtgatga ccaggtttcg
     1441 gtacatcagc gaaaccatga cctttttgtt tttcacatac ggtatggagt acaggagata
     1501 atcccggaac caggtagtga gcgatatatg ccagcggttc cagaagtccc tgagcgatcg
     1561 ggcgatcatc ggcctgttga agttgatcat caggtcataa ccgaggatac gggccgaacc
     1621 gcgcgctatg tcggtatatc ctgaaaaatc tccgtaaagt tgaaatgcaa aaaggaccgt
     1681 agccaggaga atcgtaattc ctttgtgttc gtccggattt tcatagacat aactgacaaa
     1741 tacccccagc cggtcggcga tgaccacttt tttaaagaat ccccaggcta tgagcttcaa
     1801 tccgctgacc agccgcacct ggctgaaagc ctgcgggtgg ttgacctggg ggaggagatt
     1861 gttggatcgt tcgataggtc ctgccaccaa ttgcgggaaa aaggaaacat acagggcgaa
     1921 tttccctgca tgcctctcgg ctttcgtcgt cccccggtaa acatcgaccg tataactgag
     1981 cgattgaaag atgtaaaaag agataccaac cggcagcaga atgtccagta cgggtaccct
     2041 gcccggcagg tccaaacccg ccatcgcaaa attcacgctt tccgaaaaga agttcaggta
     2101 cttgaaaccg gccagcatgc ccaggttggc ggccaggctc aggatgagat agggtttttt
     2161 ctgccgcctt gttgaaagag aatccatttt cagggcgaca aaatagtcga ccagggtagt
     2221 gaagagaata aggatagcat attccgcttt ccagcacatg tagaaaaagt aactggcaag
     2281 cagtaaaaag gcccagcgcc atttgaccgg aataacaaag tacaatgccg taaccagggg
     2341 taggaacagg aggtaatcga ccgagttaaa cagcatgagc aactgaatat taggccgcaa
     2401 atgtaagtat tttcatcaag gcattcagcc cgggaaacat agcttcaaaa aaattacctt
     2461 tgcgccatgt caaaacgaag ccttctgcgg gacgtcaccg gggtagtggc cacaaggatc
     2521 ttcctgatca cggcagggct gctgtcaagc atagtccttt caaggaatct gggcccggaa
     2581 aattttggga tttattcggc catcctggtc ttgccgctgg ttgtcgtcag ctttgcccaa
     2641 ctgggcatga gggcctcttc catttttcat atagggcgta aacagtatga acccggtgaa
     2701 atagccggca gtgtggtttt gatactggtt atgacatcca tgctgggcat actgataacc
     2761 gcccttggtt acctgatcat ggatgaagaa tcgtttacag gactttacat catcctcgta
     2821 ctgctctccc tgcctttccg gctggctatg gcttatttcg gcgggatatt cctcggtcgt
     2881 gagcaaatca gccggtcaaa tttcatcaac tggttcgccg aattcctgaa tctgctggca
     2941 gtcctggttt ttgtctggct tctgggctgg ggcataaaag gcgccctgat aagcctgctg
     3001 gcctcacagg ctactatttc aacctgggca ctttttgtga tcaggagaga catcggaatc
     3061 cggttaaaat tccatcccga agtgttgaaa agcttattgt cgatgggcgt gctgtttgcc
     3121 acatctttcg gtatcatcca gttgaattac cggatcgata ttttgttgtt gcagaaactc
     3181 tctacgatgg aggaagttgg attttattcg ctgggcgttt cgatcgccga gaagctatgg
     3241 caactgccgc tggctatcgg cgtggtgctg atgagccgga cggccaatgc cgacgatcag
     3301 cagatgatca acaggatcag tgcccggctc gtcagggtgg ctttgcttgc ggttttcctc
     3361 gcctctgtcg tgctctttgt aatatctccc tggatcgtgc cggcgatttg
//
LOCUS       DAOQXS010000345         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29275_length_3393_cov_4.083583, whole genome shotgun sequence.
ACCESSION   DAOQXS010000345 DAOQXS010000000
VERSION     DAOQXS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29275_length_3393_cov_4.083583"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..>3393
                     /locus_tag="PKM34_09400"
     CDS             154..>3393
                     /locus_tag="PKM34_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83846.1"
                     /translation="MKNLSFLLLSLAIFLLFALQGLCQQDFLPPFNFRVDSTTLMATW
                     EAPKIVLLNEDFEGDMFPPEGWADTTLGSGWQCDENPLYHAWWMVPPHTGKVALVNDD
                     WIPNNNGSLDFLYFPEMDLTFADSFSLRFDSYFDGAYGQTAYVKYSVDDGDTWLLLKQ
                     MEPSLLWQRVEIDLSEFSGTNGISNFKLAFYTNDRPDNLGWWGSGWAVDNVAVISGET
                     TNQAFSYKVFLDTTLIDCVPSTYFQYSFEYNTTHICAVMACYNNYNSDPVEQSVHSAC
                     FPKPTELGGSAPDEAVILTWSPPMVMHDGPAISIAREHGDVLRNFPSPPPISSCYGIC
                     DDGTGLWVSDPNRSGITIFKVTYDGVNTGETITLSQGQSWIGDMVSDGNYLYCCLIGG
                     SNRIGKVYLPTGEIVGTIGGDFTVEPQMGLAADFENEEFYIGGWNSNMIWRTHFNGNT
                     ISTHNINNVSGLAWSPPGSNFPVGTLWVTTYASTSWIHRIDPNSNWIQFESFMIPGGE
                     PYAGAGIEIKTSGYFQWTSLWICNQVENRIYLADIEEILIPGPQPSVPDNLLGFNLYK
                     NGEFLDYIDYTAPDSCGYVDPISSSEYYEGSVLKYEVNAVYDMTPYGFPGETEESLPE
                     GPAEIILFIYWFELDFLEDWSAGNFDDNAWYITDSNWTVSQDIGNEAPCAVFMPAAPI
                     TDYEATLESYHFLELSAHELILEYDVSLSSVNPTGNEKLQVQVFDGTNRSWNTVKTFT
                     NAEGSFDWRKDSLDIAGAFNTGRLRIRFNATGVNSSDISYWAVDNIALTRICPAPDSI
                     ETTLIPSSEDSVQVTWAEPTPPIAEWRQWDDGVPANGIGFGTSKDSWFNFAIRWTPEL
                     LIELKGASITAVGFIPYDVSACFKIALWSGDDPVPFYTQAAGNLNALQWGVFQLDTPQ
                     KIDITKDLYVGYQISTYCDYPISVDNGPAVDSLGNLFRFGNGDQWTTLLEINPEFNWN
                     LNIKAFFERDGIQYESFYRVYRGIDGSEPELITETIENELIDPVGSGTGMYCYKVQTL
                     FYNGCESEFSPESCVLITKIPFIEEVETGTVKIYPNPASEVLYIESPEKI"
BASE COUNT          906 a          730 c          800 g          957 t
ORIGIN      
        1 tggtaagatg atccgtgatt atccgtccta ttcgtgtcat ccgtgttccg atttatagaa
       61 cacgaataaa acacgaataa aacggattga caaggattca attatttcat atatttacct
      121 gtatttatat tgattaacca taaaggattg aagatgaaaa atctatcctt tcttctctta
      181 tctcttgcca tattcctgtt gttcgcgttg cagggcctgt gccagcaaga cttccttcct
      241 cccttcaact tccgggttga ttccaccaca ttaatggcaa cctgggaagc gccgaagatt
      301 gtgctactga atgaggactt tgaaggagac atgtttccgc ctgaaggatg ggccgatacc
      361 acgctgggga gtggctggca atgcgatgaa aatcctcttt accatgcctg gtggatggtg
      421 ccgccacata ccgggaaggt cgctttggta aatgatgatt ggatccccaa caataacgga
      481 agcctggatt ttctctattt cccggaaatg gacctgactt tcgccgatag ttttagcctt
      541 cgattcgatt cttattttga cggagcctat gggcaaacag catacgtaaa atatagcgtt
      601 gatgatggcg atacctggct gttgctcaaa caaatggaac cttccttatt gtggcaaagg
      661 gttgagatcg acctttcaga attttccgga accaatggaa tcagcaattt taagctcgcc
      721 ttttatacca acgaccgacc ggacaacctg ggctggtggg gctccggatg ggcggtggat
      781 aatgtggcgg taatttccgg tgaaactacc aatcaagcct tctcttacaa ggttttcctt
      841 gatactacac taattgattg tgttccttca acttatttcc aatacagttt tgaatataac
      901 acaacccata tatgtgcagt gatggcctgt tataacaatt acaactcgga ccctgtggag
      961 caatccgtgc attcagcctg ctttccgaaa cccactgaac ttggtggttc ggcaccagat
     1021 gaagcagtga tattaacatg gtctccacca atggtgatgc acgatggacc cgcaatatca
     1081 atcgcccgtg aacacggtga tgttcttcgg aattttccat ctccgccacc catttcttca
     1141 tgttatggaa tttgcgacga tggaaccggg ttgtgggtga gtgaccctaa caggtcaggt
     1201 ataactatat tcaaagtgac ttatgatgga gtaaataccg gtgagacaat cacattaagt
     1261 caaggccagt catggatcgg ggatatggtc agtgacggta attatctcta ttgttgcctg
     1321 attggaggat caaacaggat cggtaaggta tatctgccta cgggtgaaat cgtcggtaca
     1381 ataggcggag acttcaccgt agaaccccag atgggcctgg ctgctgattt tgagaacgaa
     1441 gagttctata tcggcggctg gaacagtaac atgatctgga ggacccattt taacggaaat
     1501 actatctcaa cccataacat taataatgtt agcggactgg cttggagccc tccggggagt
     1561 aattttcctg tgggaacatt gtgggtgacg acatatgcaa gcacttcctg gattcatcgg
     1621 attgatccga actctaattg gattcaattc gagtctttta tgattccggg cggtgaaccc
     1681 tatgcaggtg ccggcattga aatcaaaaca agcggatatt ttcaatggac ttcactctgg
     1741 atatgtaacc aggttgagaa tagaatctat cttgcggata ttgaggaaat attaatccct
     1801 gggccgcaac cttctgtccc cgataacctg ttaggtttca acctctataa aaacggcgaa
     1861 ttccttgact acatcgatta tacggctcct gatagctgtg gttatgtcga cccgatcagt
     1921 agtagcgaat attatgaagg atcagtcctg aaatacgaag tcaatgcagt ctatgatatg
     1981 accccgtatg gttttcccgg ggaaacagaa gaatctttgc ctgagggccc tgcggaaata
     2041 atattattca tttattggtt cgagctggat ttcctggaag attggtcggc cggtaacttc
     2101 gatgacaacg cctggtatat caccgacagc aattggacgg tcagccagga catcggtaat
     2161 gaagctccct gcgccgtatt tatgcctgct gctccaataa ctgattatga ggccacacta
     2221 gagagttacc acttcctgga gctttcggct catgagttaa tccttgagta cgacgtgtct
     2281 ttatcgtcgg taaatcccac cggaaatgag aaattgcagg tacaggtatt cgatggcaca
     2341 aaccggtcat ggaatacggt aaaaacattt actaatgccg aaggaagttt tgactggcga
     2401 aaggatagcc tggatatcgc cggggcattt aatacaggga ggttaaggat caggttcaat
     2461 gccaccggtg taaattcttc cgatatcagt tactgggctg tggataatat tgctttaacc
     2521 cgcatttgcc ctgctcctga ttccatcgaa acaactttaa taccatcatc agaagattcc
     2581 gtccaggtga cctgggcaga gcccacacct ccgattgccg aatggaggca gtgggatgac
     2641 ggggttccgg ccaatgggat tggttttggt acttcaaaag attcatggtt taattttgcc
     2701 attcgctgga ctcctgaatt attgatagaa ttaaaaggtg ccagcattac tgctgtagga
     2761 tttattcctt atgatgtgtc agcctgtttt aaaatagcgc tttggtcggg tgatgaccct
     2821 gtgccttttt acacccaggc tgccggaaac cttaacgctt tgcaatgggg tgtctttcaa
     2881 ctcgatactc cgcagaagat agatattaca aaagaccttt atgtaggtta tcagatttca
     2941 acttattgtg attatccgat ttcggtcgat aacggcccgg ccgttgacag tcttggcaat
     3001 ttattccggt ttggtaatgg cgatcaatgg acgacattac ttgaaataaa tccggaattt
     3061 aactggaacc tgaacatcaa agcctttttc gaaagggatg gcattcagta tgaaagcttt
     3121 tatcgtgttt atcgcggcat agacggaagt gagccggaac tgatcacaga aacgatagaa
     3181 aatgaattga tcgatccggt cggctccgga acaggtatgt attgctataa agtacaaacc
     3241 cttttttaca acggttgtga gtccgaattc tctccggaat cttgtgttct gattacaaag
     3301 attcctttta ttgaggaagt tgaaaccgga acagtgaaaa tctaccccaa ccccgcgtcg
     3361 gaggtgcttt acatcgaatc tccggagaag atc
//
LOCUS       DAOQXS010000346         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29511_length_3375_cov_4.400904, whole genome shotgun sequence.
ACCESSION   DAOQXS010000346 DAOQXS010000000
VERSION     DAOQXS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29511_length_3375_cov_4.400904"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..948
                     /gene="fabD"
                     /locus_tag="PKM34_09405"
     CDS             55..948
                     /gene="fabD"
                     /locus_tag="PKM34_09405"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNQ83847.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLSEGSALARELFSKANSILGFNITE
                     IMFEGTDEDLRQTKVTQPAIFLHSVILSRVLGNEFKPDLVAGHSLGEFSALVANKVLS
                     FEDGLRLVSKRAMAMQKACELKPSTMAAVIGMEDTDVERVLSLIKDEIVVPANYNCPG
                     QLVISGSVQGIETAAQKLSEAGAKRVMTLNVSGAFHSPFMQPAAEELANAINTTKFSK
                     GICPIYQNVTGQGVTDPEIIKKNLIAQLTSPVRWSQSVKNMIASGVTHFTEVGPGTVL
                     QGLIKKVKKDVVTESAEVKRS"
     gene            complement(1017..1769)
                     /locus_tag="PKM34_09410"
     CDS             complement(1017..1769)
                     /locus_tag="PKM34_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83848.1"
                     /translation="MRISMVFGNIFSSARSSLAGFSALGTDMHSHLIPGIDDGARTLD
                     DSVNLIRSMAGLGFKKLITTPHIQHDFYRNTREIILNGLDTVRNAIRSENIPVEIEAA
                     AEYLLDDGFETLAENGDLLSFSGKHILVELSYFNPNPNLKNLVFNLQCDGYKVILAHP
                     ERYTYWFSDFGKFEDLKDRGIFFQLNLVSLTGFYPDPVKKFAEKLIDKGMYDFAGSDM
                     HNMNYMNALEKSLKEKSLKKLLESGKLRNSIL"
     gene            complement(1744..2811)
                     /gene="rfbB"
                     /locus_tag="PKM34_09415"
     CDS             complement(1744..2811)
                     /gene="rfbB"
                     /locus_tag="PKM34_09415"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ83849.1"
                     /translation="MTNKLHRTIIITGGAGFIGSHVVRLFVNKYPEYRIINLDALTYA
                     GNLENLRDVEGKPNYTFVRADISNEAEVRQVIRQFHPDGIIHLAAESHVDRSISNPTE
                     FVFTNIVGTVNLLNAAREIWKDNPEGKRFYHISTDEVYGSLGDTGFFTESTPYDPKSP
                     YSASKASSDHLVRAYHHTFGLPAVISNCSNNYGPNQFPEKLLPLFINNIRQNKPLPVY
                     GKGDNVRDWLYVEDHAAAIDLIFHRGRDGETYNIGGNNEWKNIDLIRLLCRLMDDRLD
                     RPPGTSEKLIAFVKDRAGHDLRYAIDSGKLQNELGWKPSVTFEQGLARTVDWYLANQQ
                     WLDHVSSGDYLKYYENQYGVR"
     gene            complement(2889..>3375)
                     /locus_tag="PKM34_09420"
     CDS             complement(2889..>3375)
                     /locus_tag="PKM34_09420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ83850.1"
                     /translation="LRYFNPIGAHESALIGEAPSDIPDNLVPYITQTATGKRPYLRVF
                     GNDYNTPDGTPVRDYLHVTDLAKAHLAALNRMNEGKMKEKFEVFNLGTGKGYSVMDII
                     SSFEKVAEMKLNYKVVARRPGDVEQIWADPTLANTELGWKAEKTLDEMLLSAWNWQKA
                     K"
BASE COUNT          810 a          813 c          900 g          852 t
ORIGIN      
        1 aagaaatttg cctacttttg gcgcacttat aaagagataa ttttagtacg acgcatgaaa
       61 gcatatgttt ttccaggcca gggggcccag tttgtcggga tgggtaagga tttgtcggag
      121 gggtcagcgc ttgcaaggga gctgttcagc aaagccaatt ccatactcgg attcaatatt
      181 accgagatca tgtttgaagg gaccgatgag gaccttcgcc agaccaaagt aacacagccg
      241 gccatatttc ttcattcggt gatcctgtcg cgtgttttgg gcaacgaatt caaacccgac
      301 ctggtagccg ggcattcgct gggtgaattt tccgctctgg tagccaacaa ggtactttcg
      361 ttcgaagacg gcctccggct ggtgtcaaaa cgggctatgg ccatgcagaa ggcctgcgag
      421 ttaaaaccct cgaccatggc ggctgtcatc ggaatggaag acaccgacgt agaacgggta
      481 ctcagcctga tcaaggatga gatcgtggta ccggccaact acaattgccc ggggcagctg
      541 gtcatttcgg gcagcgtgca agggattgag accgcggcgc aaaagctgtc ggaagcgggc
      601 gccaagcgtg tcatgacgct gaatgtcagc ggcgctttcc attcgccgtt catgcagccc
      661 gcagcggaag agctggccaa tgcgatcaat acgaccaaat tcagcaaagg catatgcccg
      721 atctaccaga atgtgaccgg ccagggcgtg accgatccgg agatcatcaa gaaaaacctg
      781 atcgcgcagc tcacctcccc cgtccgttgg tcgcagtcgg ttaaaaatat gattgccagc
      841 ggcgtcaccc atttcaccga agtaggcccg ggaacggtgc tccaggggct gatcaagaaa
      901 gtaaagaaag acgtggtgac tgagagcgcg gaggtgaaaa ggtcgtaagc cgttcgctgg
      961 tcccgaggag ttcgcgacga gggatcgttg gtcattagca ggtgcagcag gatatatcag
     1021 agtatactgt tccggagctt tcccgattcg agtaattttt tcagtgattt ttccttcagg
     1081 cttttctcca gcgcattcat gtaattcatg ttatgcatat cgctgccggc aaaatcatac
     1141 atacctttat caataagctt ttcagcgaat tttttaaccg ggtcagggta gaaaccggtc
     1201 aaggagacca ggttaagttg aaaaaagatg ccccggtctt tcagatcctc gaactttccg
     1261 aaatcactaa accaataggt atagcgctcc ggatgcgcga gaatcacctt gtatccgtcg
     1321 cattgtaaat tgaataccag gtttttaagg tttggattcg gattaaagta ggataattca
     1381 accaatatgt gcttcccgct gaaactcagc aaatcgccat tctcagcgag cgtttcgaaa
     1441 ccatcatcca ggaggtactc agcagccgct tcaatttcaa cggggatgtt ttctgaccgg
     1501 atggcatttc tgacggtatc cagtccattg aggatgatct cccgggtatt gcggtaaaaa
     1561 tcgtgctgaa tgtgtggcgt ggtgatgagt tttttaaaac ccaggccggc catgctccgg
     1621 atgagattga cggaatcatc cagtgtccgt gcgccgtcat cgataccggg aatcaggtgg
     1681 gagtgcatat cggtgccaag agcagagaag ccggccagag aagatcttgc tgatgaaaaa
     1741 atattaccga acaccatact gattctcata atatttcaga taatcgcctg aagaaacatg
     1801 atcgagccat tgctggttgg caaggtacca gtcgactgtt cgggcgaggc cctgctcgaa
     1861 tgtaaccgag ggtttccaac ccaattcatt ttgcagttta cccgagtcga tggcatagcg
     1921 caggtcatgg ccggcacggt ctttcacgaa agctatcagc ttttccgatg taccgggcgg
     1981 gcggtccagc cggtcgtcca tcagcctgca aagcaggcgg atcaggtcga tatttttcca
     2041 ttcgttattc ccgccgatgt tataggtctc tccatccctg ccccggtgaa agatcaggtc
     2101 gatagccgcg gcatggtctt caacataaag ccagtcgcgg acattatccc ctttaccata
     2161 aacgggtagc ggtttattct gtcggatatt attaatgaaa agaggaagca gtttttcggg
     2221 gaactggttg ggcccgtagt tattggaaca gttggagatc actgccggca ggccaaaagt
     2281 gtgatgatag gccctgacca ggtggtcgct gctggctttg gaggcagaat agggactttt
     2341 cggatcgtag ggagtggatt cggtaaaaaa accggtgtcg cccagcgaac cgtaaacctc
     2401 atccgttgat atatggtaaa accttttccc ttccggattg tccttccata tctcccgtgc
     2461 ggcattcagc aggttaaccg ttccgacaat attcgtaaaa acaaattctg tcggattgga
     2521 gatcgaccgg tcgacatgcg attcggcggc caggtgaata atgccatcgg gatgaaactg
     2581 acggattact tgcctgacct ctgcttcgtt tgaaatatca gccctgacaa aagtataatt
     2641 cggtttgccc tccacgtcgc ggaggttttc caggttaccg gcatacgtca gggcatcgag
     2701 gttgatgatc ctgtattccg gatatttgtt tacaaacagt cgaaccacat gcgatccgat
     2761 gaatccggcc cctccggtga tgatgatggt tcgatgcaac ttgtttgtca ttgtagttaa
     2821 ggtttgttta taattgtcat aagctcactt cgttcgcgtc agtggctcgc tgcgctcgcg
     2881 tcagtggttt attttgcttt ctgccagttc caggccgaga gcagcatttc atcgagtgtc
     2941 ttttcggctt tccagccgag ttccgtgtta gcgagcgtcg gatcggccca gatctgctcg
     3001 acatctccgg gccggcgtgc gaccacctta taattgagct tcatttcagc cactttttca
     3061 aaggaactga taatatccat tacggaatat cctttcccgg ttccgagatt gaaaacctcg
     3121 aatttctctt tcattttacc ttcattcatc cggttcaatg ccgccagatg ggctttggcc
     3181 aggtcagtaa catgaaggta atcccttacc ggcgttccgt cgggggtatt gtaatcgttg
     3241 ccgaaaaccc ggagataggg gcgtttgccg gtggcagtct gggtgatata gggcaccagg
     3301 ttatcgggaa tatcggaagg ggcctccccg atcagcgcag attcatgggc accgatcgga
     3361 ttgaaatacc ggagg
//
LOCUS       DAOQXS010000347         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29552_length_3372_cov_3.055170, whole genome shotgun sequence.
ACCESSION   DAOQXS010000347 DAOQXS010000000
VERSION     DAOQXS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29552_length_3372_cov_3.055170"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKM34_09425"
     CDS             complement(<1..670)
                     /locus_tag="PKM34_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ83851.1"
                     /translation="MKSAEEIYALFQKHPGISTDSRDIRPGTLFFAIRGSHFDGNRFA
                     NDALNNGAAYAIVDDPDSVSDQRYILVDDTLATLQEIAMIHRSGIRAKVIGITGSNGK
                     TTTKELTSRVLSNGYNTLATKGNFNNHIGVPLTVLSITPQTQLAVIEMGANHPGEIAA
                     LCQVARPDYGIITNIGKAHLEGFGSYDGVIKAKSELYDFLRTSGGTAIVNQDDPLLIK
                     LSKGL"
     gene            complement(765..2252)
                     /locus_tag="PKM34_09430"
     CDS             complement(765..2252)
                     /locus_tag="PKM34_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ83852.1"
                     /translation="MIRFKSLSVIFLAGVLLAFLASCQQQGASRKASRTTGWKYNDPS
                     VGGFEVSSAREQATGPGLVLVEGGRFTMGANFENLTYEWDNIPKTVTVPSFYLDQTEV
                     SNIDYLEYLYWLERVFGEDYPEVVRNARPDTLVWRERLAFNEPLVENYLRHPSYQDYP
                     VVGVNWIQANDYCLWRTDRVNEMILISEGYLDFDPDQKNRDNFNTDAYLAGQYIGVVN
                     KPKANLNPSSTGERSINLQDGILLPKYRLPTEAEWEFAALGLMGNTLYERVVERRIYP
                     WNGNLLRTDQKKYYGSFISNFKRSSGDYMGVAGNLNDGADLPAPVGSYWPNDYGLYNM
                     AGNVAEWVMDVYRPLSPEDVADYAPFRGNVFTKPATDADGFLLEPDSLGRMQRIPVTD
                     EESKDRVNYRKADNINYADGDYQSVSSSDWLTDPGKNTTDEMYEFGATSLITDKSRVI
                     KGGSWKDGPYFLSPGTRRFMDEKLSTNWIGFRCAMVRVGDPMKGR"
     gene            complement(2283..3287)
                     /locus_tag="PKM34_09435"
     CDS             complement(2283..3287)
                     /locus_tag="PKM34_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNQ83853.1"
                     /translation="MLQRILTILTVVTALLFNYKGQAQDPEFSQFYGNPLYLNPALAG
                     AEICPRAVLNYRNQWPGLAKSFVTYNASYDQYFKKLHGGVGVLFNMDNAGNGILKTTQ
                     ASLMYAFSLQASKNLFINMALQGTFYQKSLNWDMLDFYDEIDPQQGFIIPTSEVPPNN
                     NTVIQPDFSAGVVFGWKGILHGGFAAHHLTQPELAFYTENVNKLPMKYTGHLGANINL
                     EGGGMGFAEDVEPTFYIAPNLLYQQQGEFHQLNAGVYVIRLPIIVGAWFRHNFENSDA
                     VIALVGLNYKNLKVGYSYDITLSKLKSNTGGAHEISVAYQFNCIEKKRKIHAIKAPGF
                     "
BASE COUNT          824 a          784 c          812 g          952 t
ORIGIN      
        1 tcaaaccttt tgataatttt atcagcagag gatcatcctg gttgacaatg gcggtgccgc
       61 cgctggttct cagaaaatca tatagttccg attttgcttt gattacgcca tcatagctgc
      121 caaatccttc caggtgtgct ttccctatat tagtaatgat tccatagtca ggacgggcga
      181 cttgacaaag ggcggctatc tctcccggat ggttggcccc catttcgatt acggctagtt
      241 gtgtttgggg tgttatggaa agaacagtca gtggtacccc gatgtggtta ttgaagtttc
      301 cttttgttgc gagtgtgttg tatccgtttg agagcacacg ggatgtcagt tctttggtgg
      361 ttgttttgcc gttcgatccc gtaataccga tcacttttgc tctaatcccc gacctgtgga
      421 tcatggctat ttcctggagg gtcgccaggg tatcatcaac aaggatatat ctctggtcgg
      481 aaactgaatc cggatcgtcg acgatcgcat aagcggcccc gttgttcaat gcatcgtttg
      541 caaaccggtt accgtcgaaa tgagaacccc ggatagcgaa aaacagggtt ccgggccgga
      601 tgtcccgtga gtcggtggaa attcccgggt gcttttgaaa cagggcataa atttcttcgg
      661 cagatttcat agaaagaaat tcatagaaat tgacaaagat aaaaaaagag gctgttcaaa
      721 gattttaaac agcctccgga tttgaagctt caggctttaa tgttttatct ccctttcatg
      781 gggtctccga cgcgaaccat agcacaccgg aaaccaatcc agttggtgga aagtttttca
      841 tccataaatc ttcttgttcc cgggctgagg aaatagggac catctttcca ggaaccgcct
      901 ttgatcactc tggatttatc ggtgattagg gatgttgccc caaactcgta catctcgtcg
      961 gtagtattct tacccgggtc tgtgagccaa tccgaagagg agaccgactg atagtcgcca
     1021 tcggcataat taatattatc ggctttacgg taattaacgc ggtctttgct ttcctcgtcc
     1081 gtcacgggga ttctttgcat tctgcccagg ctgtcgggtt cgagcagaaa tccatcggca
     1141 tcggtagctg gtttggtaaa gacgttaccg cggaaagggg cataatcggc aacatcttcc
     1201 ggtgacaggg gacgatacac atccataacc cattcagcca cattaccggc catgttataa
     1261 agcccatagt catttggcca gtaactgcca acaggtgcgg gcaggtccgc tccgtcgttc
     1321 aggtttccgg caacccccat atagtcgccg ctcgaccttt tgaagttgga aataaagctt
     1381 ccgtagtatt ttttctgatc agtgcggaga aggtttccgt tccaggggta aatgcgtcgt
     1441 tcgacaaccc tctcatataa ggtgtttccc ataagcccca gcgctgcaaa ttcccattcg
     1501 gcttcggttg ggagcctgta tttgggtaag aggataccgt cctggagatt gatggagcgt
     1561 tctcctgtgc tgctagggtt gaggtttgct ttgggtttgt tgaccactcc aatgtattgc
     1621 cctgccaggt atgcatcggt attgaaatta tcacggtttt tctgatcggg gtcaaaatcg
     1681 agataacctt ctgaaatgag gatcatttca ttgaccctgt cggtacgcca caggcagtaa
     1741 tcgtttgcct ggatccagtt aacgcctacg accgggtagt cctgataaga cggatgcctg
     1801 aggtagtttt ccaccagagg ttcgttaaaa gccaggcgct cacgccagac cagcgtgtcg
     1861 gggcgggcgt tccggacaac ttccggatag tcttccccga agacccgttc cagccagtat
     1921 aggtattcca ggtagtcgat attgctcact tcggtctggt ccaggtaaaa tgagggaacc
     1981 gtaaccgttt tgggtatgtt atcccattca taggtcaggt tctcaaaatt agctcccatg
     2041 gtaaaacggc caccttcgac caataccagg cccgggcccg ttgcctgttc cctggcggat
     2101 gaaacttcga atccaccgac agagggatca ttgtatttcc atcctgtagt tcttgaggct
     2161 ttccttgagg caccctgctg ctggcaggag gccaggaatg caagcaagac gcctgccaaa
     2221 aaaattacgc tcaaactttt aaatctgatc atataaggta ttcattgaat ttcgacatat
     2281 aactaaaaac cgggtgcttt tattgcatga atctttcttt tcttttcgat acagttgaac
     2341 tgataggcaa ctgagatttc atgcgccccc ccggtattgc ttttcaattt ggacaatgtg
     2401 atgtcgtaac tatatcccac cttgagattt ttataattca gtccgacgag tgcgatgacc
     2461 gcatcagaat tctcaaaatt gtgccggaac caggccccga cgatgatcgg caggcggatc
     2521 acataaaccc cggcgttcaa ctggtgaaat tctccctgct gctgatacaa caggttaggt
     2581 gcaatataaa aggtaggttc gacatcttca gcaaatccca ttcctccacc ttcaaggttt
     2641 atattggctc caaggtgtcc ggtatatttc attggaagtt tattgacatt ttcggtataa
     2701 aatgccagtt ccggctgggt caggtgatgg gccgcaaaac ctccgtgcag aatccctttc
     2761 cacccgaata ccactccggc ggagaagtcg ggctgaataa cggtattgtt attcggcggg
     2821 acttccgagg tgggaatgat gaatccttgc tggggatcta tttcatcata aaaatccagc
     2881 atatcccagt tgagtgattt ttggtaaaag gtgccctgca aagccatgtt gatgaacaaa
     2941 tttttcgaag cctggaggct gaaggcgtac atcaggctgg cctgggttgt tttcagaatg
     3001 ccgttgcccg cattatccat attgaaaagt acgccgaccc caccgtgcag ttttttgaaa
     3061 tactggtcat aggaagcatt ataggtgacg aaacttttgg caagcccggg ccactgattc
     3121 ctgtaattga gtactgcccg cgggcagatt tcggctcctg ccaatgccgg attcaggtat
     3181 aacggattgc cataaaactg tgagaattcg ggatcctgtg cctgcccctt gtagttgaac
     3241 aaaagtgctg ttacaacagt taatatagtt aaaattcttt gcaacatcaa tccggcctta
     3301 aatttgaggg tacaattata caatattttt taattcaaaa attttttttt gaatttatta
     3361 caatgacaat at
//
LOCUS       DAOQXS010000348         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29565_length_3371_cov_4.190591, whole genome shotgun sequence.
ACCESSION   DAOQXS010000348 DAOQXS010000000
VERSION     DAOQXS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29565_length_3371_cov_4.190591"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PKM34_09440"
     CDS             complement(<1..1756)
                     /locus_tag="PKM34_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83854.1"
                     /translation="MIDNRIHFQDKNPGIPSDELLSIARPRPNHKFLGLARVKLYFYK
                     LGTHGKPESKFRTWLREKAGERPSVFDSASSLQSIAEMKQYLNKTGYFFSSVEFRTNH
                     LRKKKVVVDYLVTPSVPYRVKSVRYDIHDSIMLSFIEKSIADSRIITGDKYNAFNLDD
                     ERDRMTEILQNNGYFYFTRDYIYFEIDSAFRSNELEINVKVKPWMDDTLKGIAQVHKR
                     YFIRNVFITPGFNPMAPVTAKQDTIGLEIPGALRKKYSGDYFLIQEKDDHIHPRTIIQ
                     SVFIRPGDPFRQVNIGRTRSRLSEIGVFGYTNIRFKPVTGLPDTITDGILDCQIDLTK
                     RKPHSFTVETEVTNSGGRPGIGLNFTYTNSNIFRGSETFRMKARGALEAQKIFGSNAE
                     YLDGTPFFNTIESGLEMGLLFPRFLIPVRQERFPRYFRPKTTISLGIGYEKRPEFERW
                     LTNLTFGYDWKESERKRHQIFPFDWSLVNVNLSPDFKQVLEDEPNDRIKNQYTDNFIT
                     AIRYSYTYNTQDIKKIRDFFFFKGNLETAGNILNLGSNLLRAPVDSLGRYILFGIPFA
                     QYVKIDGDFRFFNVITRNT"
     gene            1985..2536
                     /locus_tag="PKM34_09445"
     CDS             1985..2536
                     /locus_tag="PKM34_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ83855.1"
                     /translation="MLALFAKAVLKIMGWRITGVIPPGTRKCVIAVAPHTSYWDFVIG
                     RLAYWVMGIKASFLIKKEVFRFPFKFLLLSMGGIPVDRGRSSKMVEQVAEQFRNADSL
                     YIVITPEGTRKPVRNWKKGFYYIAMQAQVPIALGYLDYARKEGGVGKVIFPNGDYDSQ
                     LKEIKEFYRSMTPKHPEKFILGD"
     gene            2968..>3371
                     /locus_tag="PKM34_09450"
     CDS             2968..>3371
                     /locus_tag="PKM34_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83856.1"
                     /translation="MKRILFSGLISIFLCSVAIAQVSINPNGSPPHPSAGLEISFPNK
                     GFLLPRLTFEQMNNIPEPAEGLLIYCVDCNTDGSGSLAIFQNQAWRAFNLNCAKPNTP
                     TSGIKVPATTSIEWKWNPVPIAEGYKWGLTPD"
BASE COUNT          885 a          731 c          765 g          990 t
ORIGIN      
        1 atgtattcct ggtaataaca ttaaagaacc tgaaatcgcc atcgatcttc acatactggg
       61 caaaggggat accaaataaa atgtacctgc ctaaagaatc aaccggtgcc cggaggaggt
      121 tacttcccag attgagtatg ttgccggcag tttccaggtt tcctttaaag aaaaagaaat
      181 cacgtatctt tttaatatcc tgtgtgttgt aagtgtaaga gtaccggatg gctgtgataa
      241 agttatccgt atactgattt ttgatcctgt cgttgggttc atcctctaag acttgcttga
      301 aatcgggtga gaggttcaca ttcaccaggc tccagtcgaa cgggaatatt tggtgccgct
      361 tccgttccga ctctttccag tcataaccaa aagttaggtt ggtcagccat cgttcaaact
      421 cgggccgttt ttcataaccg atacccaggc tgatggttgt tttgggtctg aaatacctgg
      481 gaaaacgctc ctgcctcaca gggatcagga accggggaaa taaaaggccc atttccaggc
      541 ctgactcaat agtattgaag aatggggtgc catccaggta ttctgcatta ctgccgaata
      601 ttttttgcgc ttcgagcgct ccgcgtgctt tcatccggaa tgtttccgat cccctgaaaa
      661 tattactgtt ggtataggtg aaattaagcc cgatccccgg ccttcctccc gaattggtca
      721 cttcagtctc aacggtgaaa gagtgcggct ttcgttttgt aaggtctatc tggcaatcca
      781 gaatcccgtc tgtgatcgtg tccggtaatc ctgtgacagg tttgaacctg atattggtat
      841 acccgaaaac accgatctct gacaacctgg agcgggttct tccgatgttc acctgcctga
      901 aaggatctcc cggccggata aaaaccgact gaataatggt acgtggatga atgtgatcat
      961 ctttctcctg gatgaggaaa tagtcaccgc tgtatttctt tcttaacgcc cccgggatct
     1021 caaggccgat agtatcctgt tttgctgtga ccggggccat tggattgaaa ccgggggtga
     1081 tgaacacatt gcgtatgaaa taccttttat ggacctgtgc aatacccttc agcgtatcat
     1141 ccatccaggg tttgaccttt acgttaattt ccagttcatt gctccgaaat gcactgtcga
     1201 tctcaaagta aatataatct cgtgtaaagt aaaaatagcc gttgttctgc agaatctcgg
     1261 tcatcctgtc ccgttcatca tccaggttaa aggcattgta tttatcccct gttattattc
     1321 tactatcggc tattgatttt tcaatgaatg aaagcattat actgtcatgg atgtcatatc
     1381 tcaccgactt tacccggtat ggtactgacg gagtgaccag gtaatctacg acaacctttt
     1441 tcttccttaa atgatttgtt ctgaattcaa cggaagagaa gaaatagcct gttttattga
     1501 ggtattgctt catctcggca atagactgca gcgatgaagc cgaatcgaaa accgaggggc
     1561 gttctccggc tttttccctg agccaggtcc tgaatttgct ttcgggtttg ccatgggttc
     1621 ccagcttgta aaagtatagt ttcaccctgg ccaggccgag gaatttatgg tttggacggg
     1681 gtcgggcgat cgaaagcaat tcatcggatg gaataccggg attcttgtcc tggaaatgga
     1741 tcctgttatc gatcaacaat tgctctccct cgcttaacag tcgcgtattt gagcatgagg
     1801 ataaaagtgc tgcaaggcag cagggcagaa ttatatatcg ggctaaatat gcgaatggaa
     1861 ccttcaccaa agcgtaccgc agcttaatgg gcaaaggtat ggatttttgt gaaaaaccga
     1921 atgaccatta tatactgtct gaccttccgg taaattttta attttgcaaa cgaatgccat
     1981 tattatgtta gcattgttcg caaaagcagt acttaaaatt atgggatgga ggattaccgg
     2041 cgttattcct ccgggcaccc ggaaatgtgt tattgctgtg gctccccaca ccagctactg
     2101 ggactttgtc attggccggc tcgcatactg ggtcatggga atcaaagcca gttttttaat
     2161 aaagaaggag gttttccggt ttccgttcaa gttcctgctc ttaagcatgg gtggaattcc
     2221 ggtcgacagg ggaagaagca gcaagatggt ggaacaggta gcagagcaat tcaggaatgc
     2281 cgattcgctc tatatcgtga tcactcctga gggaacacgc aaacccgtca ggaactggaa
     2341 aaagggattc tactatattg ccatgcaggc acaggtgccc attgccttag gctatctcga
     2401 ctatgcccgg aaagaagggg gagtgggtaa ggttatcttt cccaacgggg attatgacag
     2461 tcagttgaaa gaaataaaag aattttaccg gagcatgacc ccgaagcacc cggaaaaatt
     2521 tatcctcgga gattagaaaa caataatcgg ctaaatttca ttttaatctc cggttttagg
     2581 atttctctat tacttttgtc aacggaatat caatcggagg tccggaactc agattgcttt
     2641 cgagccacca tccaggcctt taaaacgatg cctttccggt tgaacatatt ggaaatcacc
     2701 aataacgaca ctttccagat tttgatatta cttttttggt ttttttcgat aaagttgcat
     2761 gaatgcgatt gaaacttcat ttatataccc tgatcactct gaaaggttct atttatactt
     2821 cttttccggg tcttttcctc agcggaaatc agatactaac aataatgggt tgataaataa
     2881 tcaattgcat ttattaattg ctggtttttc agaaatagct ttgttaagtt gatatgtttc
     2941 ttagtccttt aatctaaata taacatcatg aaaagaattc tattctcagg cctgatatcc
     3001 atttttctct gtagtgtcgc cattgctcag gtatcaataa accctaacgg tagcccgccg
     3061 catccttcgg cgggattgga aatttcattc cccaacaaag ggtttttact gcccaggctg
     3121 acattcgagc agatgaacaa tattcctgaa ccggctgaag gattgctgat ctattgtgtt
     3181 gattgcaata cggatggatc aggatcgctg gcgatattcc agaaccaggc ctggcgggct
     3241 tttaacctga attgtgccaa acccaatacg ccgacttccg gcattaaggt tccggctact
     3301 acctctatcg aatggaaatg gaatccggtt ccgattgctg aaggttataa gtggggtttg
     3361 acccctgatt a
//
LOCUS       DAOQXS010000349         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29681_length_3362_cov_4.006350, whole genome shotgun sequence.
ACCESSION   DAOQXS010000349 DAOQXS010000000
VERSION     DAOQXS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29681_length_3362_cov_4.006350"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKM34_09455"
     CDS             complement(<1..301)
                     /locus_tag="PKM34_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83857.1"
                     /translation="MVANQSGINTLLSFGVKSVNPEILEKLLIGRQKAADYLYDAAKS
                     ISEDGINQHILVIGQRGMGKTHLLRVLFHRIQPFIKEKKVVVAYFSEEEYGIANYF"
     gene            complement(277..1512)
                     /locus_tag="PKM34_09460"
     CDS             complement(277..1512)
                     /locus_tag="PKM34_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83858.1"
                     /translation="MKPYPLKPGRGTYPAERIVGREREITQLLRLLITQSVSVEELRR
                     MGKTLLVKKLEYICNRNLVPEEFKQEGFKVKYLSFEGIQTLGGLIDTLIREFEKIKDW
                     SKIDWARTYEFIRKIIKSQKVKIAEAEFSINLPEYKRTWQEAFFKVLEDIADAQETNG
                     SKLILIFDELPLMLWEWHKKGNAGEGIELLDILRKKRQELEEKGLRFVYCGSIGMNII
                     LKTYKKLYGYAGPATNDMEPFSVDPFTEEDAFFLCDCFEAGGFKTSNKEQCYPLIYQL
                     SNGLPFYISQMFNIIQIDFDKEVNEETVKSAYRIILNNPDYHNDFNQLKDRINIYYPE
                     NEAKVMLYILNFLSQKETIINQEEILSGLAIEDRIIKDQLYQMTKDHLLSRTQSESGT
                     WQYQFKHEIFRQWWQINQA"
     gene            1702..>3362
                     /locus_tag="PKM34_09465"
     CDS             1702..>3362
                     /locus_tag="PKM34_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ83859.1"
                     /translation="MRTYLFQLLTIAAFLYSINISAQHKTIDQKVDSVLSLMTLGEKI
                     GQLNQYNDDWGATGPVTLDTDKENQIRNGKVGSLLNCTGTVRTRLWQDIAMQSRLRIP
                     LLFGQDVVHGYKTTFPIPLAESCSWDPDAIEMAARVAAEEASACGIHWTFAPMVDIAR
                     DPRWGRVMEGAGEDPYLGSVIAFARVKGFQGNNPDGNGSLMACTKHYAAYGAAIGGRD
                     YNSVDMSERLLREVYLPPFKATVDAGVGTFMTAFNDINGVPATGNSLILRDILKGEWG
                     FEGFVVSDWGSVGEMIQHGNVKDEADAARCAITAGCDMDMESRCYIKTLEQQVNEGMV
                     PVSLIDAAAKRILKKKFELGLFADPYKNCDPERERMALENPSHTAAARDMARKSIVLL
                     KNENDLLPLSKDLKTIAFIGPLVKAVKQNKGFWDVEVPGVDSNFIVTQWEGVEAKVGA
                     KTKLLYARGCDIDGDSKDGFAEAVAVARQAEVVILSIGERRDLSGEARSRSDIGIPGV
                     QEELLKELLATGKPVVVLINAGRPLVFNYTADNAPAILYTWWLGS"
BASE COUNT          877 a          829 c          766 g          890 t
ORIGIN      
        1 caaaatagtt tgcaatgccg tattcctctt cggaaaaata ggcgactaca acctttttct
       61 ctttaatgaa aggttgaatc cgatggaaaa gaacccgcaa caaatgggtt tttcccatcc
      121 cgcgctgtcc aatgaccaga atatgctggt tgattccatc ttcagaaatt gatttcgcag
      181 catcatataa ataatctgcc gctttttgcc tcccgatcag aagtttttca aggatttccg
      241 ggtttacgct cttcaccccg aaggacagga gcgtgtttat gcctgattga tttgccacca
      301 ttgtctgaaa atttcatgtt taaactgata ttgccaagta cctgattcac tctgcgtcct
      361 gctaagtaag tgatctttgg tcatttggta aagctggtcc ttgataatcc gatcttcaat
      421 cgccagaccg gacaggatct cctcttgatt aataattgtt tctttttgtg aaagaaagtt
      481 taatatataa agcatcactt ttgcttcatt ttcaggataa taaatgttta tcctgtcttt
      541 caactggtta aaatcattat gataatccgg gttatttaaa atgatccggt aggcagattt
      601 aacggtttcc tcattgactt ctttatcaaa atcaatttga ataatgttaa acatctgtga
      661 gatataaaaa ggaagaccat tagacaattg ataaatcaac ggatagcact gctctttatt
      721 cgaagtttta aatccgccgg cctcgaaaca atcacataaa aagaatgcat cttcctcagt
      781 aaaaggatcg acactgaaag gttccatatc gttagtggca ggaccggcat aaccatatag
      841 ctttttgtat gttttaagga taatattcat cccaatagaa ccacaataaa caaaccgaag
      901 ccccttttcc tccaactcct gccttttttt cctgagaata tccagtaact cgatcccttc
      961 gccggcattc ccctttttat gccattccca aagcatcaaa ggcaactcat cgaaaatcag
     1021 gatcaatttg ctgccgttcg tttcctgtgc atcagcaata tcttccagga ctttaaagaa
     1081 agcttcctgc caggtccttt tatactccgg aagatttata gaaaattctg cctctgcaat
     1141 tttgactttc tggcttttaa ttatcttccg gataaactca tatgtcctgg cccaatctat
     1201 cttggaccag tcctttattt tttcgaattc acggatcagg gtatctatta atcctcctag
     1261 tgtttgtatg ccttcaaaag acaagtattt gactttgaat ccttcctgtt tgaattcttc
     1321 aggaacgaga tttctattac atatatattc caactttttt acaagaagtg tctttcccat
     1381 ccttcttaac tcttccacgg aaacactttg tgttataagt aatcttaaca gttgtgttat
     1441 ttctctttca cgtccaacta ttctctctgc agggtaagtt cctctcccgg gttttaatgg
     1501 atatggtttc ataattaaaa tttgagggac aaatatacga taattttatc ttacgtcaat
     1561 attatcgtac aatactttta tcgtatatta ttctactaag ctgttaaact atcaaaatca
     1621 tcagattaat tgtcctccgt tcatttatca tgatatttag taattttatt ttgataaaaa
     1681 ccagaaatta tccatttgaa tatgagaact tatttattcc aactgctcac aatcgcagca
     1741 tttctttact ccataaatat ctccgcccag cacaaaacca tcgaccagaa ggtcgattcg
     1801 gtgctgagcc tgatgacgct gggcgaaaag atcggccagc taaaccagta taacgacgac
     1861 tggggagcta ccggcccggt gacgctcgat accgataaag agaaccagat caggaacgga
     1921 aaagtaggat cgctgctcaa ttgtacaggc acggtgcgca cgcggctgtg gcaggatatc
     1981 gccatgcagt cgcggctacg gatcccgctg ctgttcggcc aggatgtggt gcacggctat
     2041 aagaccacgt ttcccatccc gctggccgaa tcgtgcagct gggacccgga tgcgatcgaa
     2101 atggcggccc gcgtggcggc tgaagaggcc agcgcctgcg ggatccactg gaccttcgcc
     2161 ccgatggtcg atatcgcccg cgatccgcgc tggggccgcg tcatggaagg ggcaggcgaa
     2221 gacccgtacc tgggatccgt gatcgccttt gcccgcgtta aaggcttcca gggaaacaac
     2281 cccgacggga acggatcgct gatggcctgt accaagcact atgccgccta tggcgccgct
     2341 atcggcggaa gagactacaa ctcggtggat atgagcgaac ggctgctccg ggaggtctat
     2401 ctgcccccgt tcaaagccac cgtcgacgcc ggcgtcggca cttttatgac cgccttcaac
     2461 gatatcaacg gcgttccggc taccggcaac agcctcattc tccgcgatat actcaaaggg
     2521 gaatggggat ttgagggatt cgtggtgagc gactggggct cggtcggtga aatgatccag
     2581 cacggcaatg taaaagatga ggccgatgcc gcccgctgcg ccattaccgc cggttgcgac
     2641 atggacatgg agagccggtg ctatatcaaa accctggagc agcaggtcaa tgaaggcatg
     2701 gtcccggttt ccctgatcga cgccgccgca aaaaggatcc tgaagaagaa gttcgagctc
     2761 gggcttttcg ccgatcctta caaaaactgc gacccggaaa gggaacgcat ggcgctcgaa
     2821 aacccgtccc ataccgccgc agcgagggat atggcgcgga aaagcatcgt cctcctcaaa
     2881 aatgaaaacg acctcctccc cctctccaaa gacctgaaga ccattgcctt tatcggcccg
     2941 ctggtcaagg cagtcaagca aaacaaaggt ttctgggatg tcgaggtccc cggtgtcgat
     3001 tccaatttca ttgtgacgca gtgggaaggc gtcgaagcta aagtcggcgc taaaacgaaa
     3061 ctcctgtatg ccaggggctg tgatatcgac ggtgacagca aagatggatt tgctgaagcc
     3121 gttgcggtag cgcgccaggc cgaagtcgta atcctgagca tcggtgagcg gagggacctg
     3181 tcgggcgagg ccaggagccg aagtgatatc ggaattcccg gagtgcagga agagctgttg
     3241 aaggagctgc tggcgacggg caaaccggtg gtggtgctga tcaacgccgg ccggccgctg
     3301 gtatttaact acacagccga caacgccccg gccatcctct acacctggtg gctcggcagc
     3361 ga
//
LOCUS       DAOQXS010000350         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_29882_length_3347_cov_3.305589, whole genome shotgun sequence.
ACCESSION   DAOQXS010000350 DAOQXS010000000
VERSION     DAOQXS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29882_length_3347_cov_3.305589"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKM34_09470"
     CDS             complement(<1..256)
                     /locus_tag="PKM34_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H1/viroplasmin domain-containing protein"
                     /protein_id="HNQ83860.1"
                     /translation="MAEKKFYVVWKGKKAGIYDTWKECEAQVKGVEGARYKSFPGKQE
                     AERAFSAGWVKPAGKRVSNPVSKAGVSNPVWDSIAVDAACS"
     gene            316..786
                     /locus_tag="PKM34_09475"
     CDS             316..786
                     /locus_tag="PKM34_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNQ83861.1"
                     /translation="MMNKYTLLLLLCIALLTSCGPRKIYEKHYDIPRITWSRFDVKTF
                     DVEIKDIGASYDFYIAIRHHTEIPFKNIETKLTIYTPSGEMRMNEQKIVLRDNEGKLL
                     GSGMGDLWDIKYPARKGLKFTEKGTCRVEISSTMSKADLPGIIQVGLIVEKGNN"
     gene            complement(844..1770)
                     /locus_tag="PKM34_09480"
     CDS             complement(844..1770)
                     /locus_tag="PKM34_09480"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNQ83862.1"
                     /translation="MKIYHSIDLFQPVPNPVVTVGTFDGVHRGHQAIFDAMRNEALRC
                     GGETVVVTFYPHPRLVIHPDSKSLRFINTQERKYEIISRSRIDHLIIIPFTKEFSNLS
                     SGEFVRRYLVEKIKIHQLIVGYDHHFGKDRLGGYNELKGLGKIHGFEVSEVPAMIIDG
                     IPVSSTKIRNALIEGNIRLANSLLGYNYSISGKVVYGNRIGRTIGFPTANIEQEDEYK
                     LISAVGVYACLVDHHGKTYKGMGNIGYRPTIDMGSLTIEVNIFDFDEEIYGDRIIIYF
                     IERIRDEVKFENLTALRDQLVSDRAKVLEILR"
     gene            complement(1847..2848)
                     /locus_tag="PKM34_09485"
     CDS             complement(1847..2848)
                     /locus_tag="PKM34_09485"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ83863.1"
                     /translation="MKICVVGATGLVGSEMVKVLDESGPGDFEFFPVASERSAGKMVS
                     FRGKEHPVIGMQEAVEKRADIALFSAGAAASRQWAPLFAAAGTTVIDNSSAWRQDPEI
                     PLIVPEINAHILNSSHKIISDPNCSTIQMVMVLAPLHRRFTIRRVVVSTYQSVTGSGA
                     KGYLQLENERKGIDGPKFYPHTIDLNVIPHGGDFTDNGYTTEEVKLDFETRKIIGDDS
                     IKVTATVVRVPVYGGHSESVNVEFEQPFETEEVLRILANEKGIAVIDHPAKAEYPMPI
                     TARGKDEVFVGRIRRDLTVANGLNMWIVSDNLRKGAATNAVQIAEYLLENKLVGLDM"
     gene            2949..>3347
                     /locus_tag="PKM34_09490"
     CDS             2949..>3347
                     /locus_tag="PKM34_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83864.1"
                     /translation="MKNHILFFVLLPVCLAAQVRDDFSDGDFTTGPAWQGDLGHFTLS
                     YSSAVPENQRPALQLNAPAAGISSLRLAEPFSGDLEWQFWIKMSFNTSSSNFARIYLM
                     TDSPDPQQPLNGYFIQAGGTSDSVSFWRQDS"
BASE COUNT          791 a          911 c          801 g          844 t
ORIGIN      
        1 cgctgcaggc ggcatctaca gcgatgctgt cccagacagg gttggaaacc cctgctttgg
       61 atacagggtt ggaaaccctt tttccggccg gctttaccca gcccgctgaa aatgcccttt
      121 ctgcctcttg ctttcccgga aacgacttgt accgggcccc ctcaacaccc tttacctggg
      181 cttcgcactc tttccacgtg tcgtatattc ccgccttttt tcccttccag accacataaa
      241 attttttttc agccatcccc gattgtttaa caggctaaaa taatcatcat ttccgttatt
      301 tttgttaaaa ggataatgat gaataaatat acgctgttat tattgctttg catagccctc
      361 ctcacctcct gcggcccccg gaaaatctat gaaaagcatt acgatatccc caggatcacc
      421 tggagccgtt ttgatgtcaa gacattcgat gtggagatta aagatatcgg ggcttcctat
      481 gatttctata ttgctatccg tcatcatacc gaaataccgt ttaaaaacat cgagacaaaa
      541 ctgacgatct acaccccctc cggggagatg cggatgaacg agcaaaagat cgtcctccgc
      601 gataatgaag gtaaactcct gggtagcggc atgggcgacc tgtgggatat aaagtacccg
      661 gcaaggaaag gactgaaatt cactgagaag ggaacgtgcc gcgttgaaat atccagcacc
      721 atgtcgaaag ccgacctgcc cggcatcatc caggtgggac tgatcgtgga gaaagggaac
      781 aattaaaagt gaacagtgaa cagtgaacag tgaatagtga aacctggaac ctggaacttt
      841 gaattatctg agtatttcca gcactttcgc ccggtcggat acaagctggt ctctcagggc
      901 cgtaaggttt tcaaacttga cctcatctct tatcctttcg ataaaataaa tgatgatccg
      961 gtcgccgtag atctcttcat caaaatcaaa aatattcacc tcaatggtca ggctacccat
     1021 gtcgattgtc gggcgatacc cgatatttcc catccctttg taagtcttgc cgtgatgatc
     1081 gaccaggcag gcatacacac caacggccga aataagctta tactcgtctt cctgttcgat
     1141 attcgcggtc ggaaagccga tggtgcgccc aatccggttg ccgtaaacca ctttgccgga
     1201 gatcgagtaa ttgtacccca gcagggaatt ggccaaccgg atatttccct caattaaagc
     1261 atttctgatc ttggtagagc tgacaggtat cccgtcgatg atcatggccg gcacctcgct
     1321 gacctcaaac ccgtgaattt ttcccagacc cttcagctca ttgtaccctc ccagccggtc
     1381 ttttccgaaa tgatggtcat agccgacgat cagctggtga attttaatct tctcgaccag
     1441 gtagcggcgc acaaattcgc ccgatgagag attggaaaat tctttggtga atgggatgat
     1501 gatcagatga tcgatccggc tccggctgat gatctcgtat ttccgttcct gggtattgat
     1561 aaagcggagg ctcttgctgt cgggatggat caccaggcgg ggatggggat aaaatgttac
     1621 aaccacagtt tcacctccgc accggagtgc ctcattgcgc atggcgtcaa aaatggcctg
     1681 atgcccgcga tggactccgt caaaggtccc caccgtcaca accgggttgg gtaccggctg
     1741 aaaaagatct atgctgtggt atatcttcaa agctttacga caactatacg actactttac
     1801 gactacttta tgacaacttt acgactactt tatgactact ttacgactac atatcaagtc
     1861 caaccagttt attttcgagc aaatactctg ctatttgcac cgcattggtc gccgcccctt
     1921 ttcgcaggtt atccgataca atccacatat tcagcccgtt agctactgta agatcgcgcc
     1981 tgatcctccc cacaaatact tcgtcttttc cccttgccgt aatgggcatc gggtattccg
     2041 ctttcgcggg atggtcgatt acggcaatcc ctttctcatt cgccagtatc ctcagcactt
     2101 cttccgtttc aaatggctgt tcgaattcca cgttgaccga ttcggaatgc ccgccataaa
     2161 ccgggaccct gacaactgtc gcggtcactt tgatcgaatc gtcgccgata attttccggg
     2221 tctcgaaatc gagcttgacc tcctccgtcg tgtagccgtt atcggtaaaa tcgccgccgt
     2281 gggggatcac gttcaggtcg atcgtatgcg ggtaaaattt cggcccgtcg atccctttcc
     2341 gttcattctc gagctgcagg tagccttttg cgccgcttcc cgtcaccgac tggtaggtgg
     2401 aaaccactac tcttctgatc gtaaaccgcc ggtgcagcgg ggcaaggacc atcaccatct
     2461 gtatcgtgga acagtttggg tcggagatga tcttgtgcga tgaattgagg atgtgagcgt
     2521 tgatttcggg gacaatgagc gggatttccg gatcctgacg ccatgccgag gagttgtcga
     2581 tcaccgtggt accggcagcg gcaaacagcg gcgcccactg gcgtgaagcg gccgccccgg
     2641 cggagaaaag cgcgatatcg gctctttttt cgaccgcttc ctgcatgccg atcaccgggt
     2701 gctccttgcc ccggaatgaa accatttttc ctgcggaacg ctctgaagcg accgggaaaa
     2761 attcgaaatc gcccgggcct gactcgtcga ggacttttac catttcactg cctacaaggc
     2821 cggtggcgcc gaccacacaa attttcatac ggggttgttt gaacggtgca aaaatagtga
     2881 atttccaaaa aaatttggaa gcgaccgggt ataaataggc caaaaatcgg attaacagag
     2941 aaaagactat gaagaaccac atccttttct ttgttctgct ccccgtctgc ctggcagcgc
     3001 aggtgcgcga cgatttctcc gacggtgatt tcactaccgg tccggcctgg cagggcgacc
     3061 tggggcattt tacgctgagc tattcatcgg cagtaccgga aaaccagcgg ccggcgctgc
     3121 agttgaatgc ccctgcggcc ggcatttcgt cgctccggct ggccgagcct ttctccggtg
     3181 acctggaatg gcagttctgg atcaaaatga gcttcaatac cagcagcagc aattttgcac
     3241 ggatctacct catgaccgat tcccccgatc cgcagcaacc cctgaacggt tatttcatcc
     3301 aggccggcgg aaccagcgac agcgtgagct tctggcgcca ggattcc
//
LOCUS       DAOQXS010000351         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30054_length_3334_cov_3.646234, whole genome shotgun sequence.
ACCESSION   DAOQXS010000351 DAOQXS010000000
VERSION     DAOQXS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30054_length_3334_cov_3.646234"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1242)
                     /locus_tag="PKM34_09495"
     CDS             complement(100..1242)
                     /locus_tag="PKM34_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNQ83865.1"
                     /translation="MNKIGATGLLAIALLMAACNEKEDVTLIPVFRPTPYEIEIPFGF
                     PTKLNIPDDNPMTVEGIELGRLLFYDGRISGQSEPGKQMSCATCHLQENAFECGLDHP
                     DFPGGKTFGVTGIPTPHYMLPLFNQVWNHSGYLWNGFVNDDNPDAMLRNIEALTYMSI
                     IAPHEMNSDTLSAVKAIKSIPGYANLFKKAFGSEEVTIDRMSKAIAQFIRTFVSADSK
                     FDQYLRGETQLSPSELNGYVLFVTEEGADCFHCHGGGGNPLFTTHLFYNNAKDSVFDD
                     PRDRFSVTGDPMMRGAYKAPSLRNIALTAPYMHDGRFKTLDEVIDFYSEGLVYTGLAD
                     PLMHHLLNGGTRLTPSEKADLKAFLLTLTDETFIKNPALSRPDSLP"
     gene            complement(1235..2077)
                     /locus_tag="PKM34_09500"
     CDS             complement(1235..2077)
                     /locus_tag="PKM34_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83866.1"
                     /translation="MSSRSGNNRVVFVALTTILLITAFTACKDPDKDKTPEYGKLTLY
                     FAHLFHDNWLVIDDSTYVNAAGNELIFNELQYFISDVVLHYSDGSEVPVNQWKEIHYV
                     DSDIPETMTWEIFDSLPPGAIDSISFVFGISEEKNQSYMYVNPPESLMFWPDILGGGY
                     HYLKLNGKWKNEENQLSPFNFHLGIGQVYDTAGHITGFIQNYFRTGARLPVYSSYALV
                     VEPGETTSVTLAMDVASWFETPDIWDFNYWGGDIMQNQEALHAGCRNGFDIFSLMSPL
                     FINE"
     gene            complement(2055..2405)
                     /locus_tag="PKM34_09505"
     CDS             complement(2055..2405)
                     /locus_tag="PKM34_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83867.1"
                     /translation="MIGIAAFMFLGSCVNENDRIVTYRITDSASGFTVRYLDESGTVI
                     SKNITVNSAQEVWNYSMISEDGGIVFVSANYKDPQSAIKVQVLIDGKIYKQSATRNDT
                     ISFVTVSGVIPVRE"
     gene            complement(2420..3310)
                     /locus_tag="PKM34_09510"
     CDS             complement(2420..3310)
                     /locus_tag="PKM34_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ83868.1"
                     /translation="MPGFTDSVESEYSNLSFSLGADWKIRDNLVIGLAAGRGVRSPDM
                     TERFIILLPIGYDNFDYLGNPGLKPEKNHQADLSVKWSVNKAGQIKAGVFYSYVTGFI
                     SGRLVPETVVKPQTKGVYGVKQFINLDKAWLTGFELEYQSPAENKWQLSAVASHTMGV
                     NPEAVRYIVSNGQVTGSEIIKNDPLSEIPPFEGTMRFSWKFFNGKLVPQANMRVVAAQ
                     ERISVSYNEKKTPAFTLAGLSVYYRFNAALEFNAGVDNLFDVAYYEHLNRNIVGSQTE
                     FYEPGINFFISAKFKIQSSK"
BASE COUNT          839 a          791 c          821 g          883 t
ORIGIN      
        1 ttcctatggt tttatgaagg tgggaaccga cggcttcagt cgtcggacta ccaattaaaa
       61 gcgaatattt gtatttttga gacagtcact gcgtcaaacc taaggtagtg aatccggtct
      121 cgaaagggcc ggatttttta tgaaagtttc atcggtgagg gtcagcagga aggctttcag
      181 gtcagctttt tctgaaggcg taagacgggt gccaccgttc agcaaatggt gcatcagcgg
      241 gtcggccagc ccggtgtaga caagcccttc ggaatagaaa tcgatgactt cgtcgagggt
      301 tttaaatctt ccatcgtgca tatatggagc cgtcagggcg atgtttcgaa gcgaaggcgc
      361 tttgtatgcg ccccgcatca tcgggtcgcc ggtcaccgaa aagcggtcgc gcggatcatc
      421 gaacacagag tccttggcgt tattgtaaaa cagatgggtt gtaaacagcg gatttccgcc
      481 gccaccgtgg caatgaaagc aatcggcccc ctcctccgta acaaacagaa catagccgtt
      541 caattctgac ggagagagtt gtgtctcacc acgaaggtac tgatcaaact ttgagtcggc
      601 ggaaacgaaa gtcctgatga actgtgcaat agctttgctc atccggtcga tcgtaacttc
      661 ctcactgccg aacgcttttt tgaaaagatt tgcatacccc gggatggatt ttattgcctt
      721 gacagccgaa agggtgtcgc tgttcatttc gtgcggcgcg atgatgctca tataggtcag
      781 cgcttcgatg ttgcgaagca tcgcatcagg gttgtcatcg ttaacaaacc cgttccacag
      841 gtatccggaa tggttccaga cctggttgaa cagtggcagc atgtaatgcg gggtagggat
      901 gccggtcacg ccaaaggtct ttccacccgg gaaatcggga tgatcgaggc cgcattcgaa
      961 ggcgttttcc tgcaggtggc aggtagcgca gctcatttgt ttgcccggtt cactttgtcc
     1021 ggatatcctg ccatcataaa acaacaaccg gcctaattca atcccctcca ccgtcattgg
     1081 attatcatcc gggatattca atttggtcgg gaagccgaag ggtatctcga tttcataagg
     1141 agttggcctg aagaccggta tcaaagtcac atcctccttt tcattgcatg cagccataag
     1201 gagggcaatt gccagtaagc cggtggcacc aatcttattc attaatgaac agaggtgaca
     1261 tgagtgagaa aatgtcaaat ccattacggc aaccagcatg caaagcttcc tggttttgca
     1321 taatatcgcc tccccagtag ttgaaatccc agatatccgg tgtttcgaac cagctggcta
     1381 catccatggc cagggttacc gaggttgttt ctcccggttc aacgaccagc gcataagatg
     1441 aataaaccgg cagcctggcg ccggtgcgga aataattctg gataaacccg gtgatatggc
     1501 ctgccgtatc gtaaacctgc ccgatgccaa gatgaaagtt gaacgggctg agctgatttt
     1561 cctcattttt ccattttccg ttcagtttca ggtagtggta accgccgccc aggatatcgg
     1621 gccagaacat cagcgattcg ggtggattga catacatata cgactggttc ttttcttcgc
     1681 tgatcccgaa aacaaagctg atcgaatcaa tcgcacccgg tggcaggctg tcgaatatct
     1741 cccaggtcat ggtttcaggg atgtcgctgt cgacataatg aatttctttc cactggttta
     1801 ccgggacctc gctgccatcc gaataatgaa ggacgacatc tgaaataaaa tactgcaatt
     1861 cgttgaatat aagttcattg cctgcggcat tcacatacgt tgaatcgtct atgaccagcc
     1921 agttatcatg aaacaggtgg gcgaaataaa gggtcagttt tccgtattcg ggggttttat
     1981 ccttgtccgg atctttgcat gccgtaaagg ctgtgatcag gagaatggtt gttaatgcaa
     2041 caaataccac ccggttattc cctgaccggg atgacacctg aaacggtgac aaaagagatc
     2101 gtatcgttcc gtgttgcgga ttgtttgtaa attttaccat cgattagtac ctgtacttta
     2161 attgctgact gggggtcttt atagtttgca gatacaaaaa caatgccgcc gtcttcagat
     2221 atcatcgaat aattccagac ctcctgtgcg ctgttgaccg taatattttt ggagataact
     2281 gttccggatt catcgaggta tctcacggta aaacccgatg cggaatcggt aatgcggtag
     2341 gtgacgatcc ggtcgttttc attaacacat gaacccagaa acatgaatgc tgcgattccg
     2401 atcaaatatc ttattttcat cactttgaac tttgaatttt gaactttgca ctaataaaaa
     2461 aattaattcc aggttcatag aattcggttt gggatccgac aatattcctg ttaaggtgtt
     2521 cataatatgc aacatcgaac agattatcaa cccctgcatt gaattccagt gccgcgttga
     2581 accggtaata aaccgataaa ccggccagtg tgaaagccgg ggttttcttt tcgttatatg
     2641 atacggaaat tcgttcctgg gcggcaacaa cccgcatatt ggcctgggga accagttttc
     2701 cgttaaaaaa tttccacgag aaccgcattg tcccttcaaa aggaggaatc tcgctcaggg
     2761 ggtcgttttt gatgatttcg ctcccggtaa cctgaccatt ggaaacaata tagcgcacag
     2821 cttcaggatt gacacccatg gtatggctgg cgacagctga gagttgccat ttgttttctg
     2881 ccggagactg gtattccagt tcgaatccgg tcagccaggc tttgtcgagg ttgataaact
     2941 gcttgacgcc gtaaacgcct ttggtttgcg gcttgacgac agtttcaggc accagccggc
     3001 ccgagataaa accggtgaca taagaataaa aaacgcctgc cttgatctgg cctgctttat
     3061 ttaccgacca tttcaccgaa aggtcggcct ggtggttctt ttccggtttt aaacccgggt
     3121 tgcccaggta atcgaaatta tcgtaaccga tgggcaacag gatgataaac cgctcggtca
     3181 tatcggggct ccttactcca cgtccggcgg ccagtccgat aaccaaattg tctcttattt
     3241 tccagtcggc tcccaggctg aagcttaaat tggaatattc cgattcaacg ctgtcggtga
     3301 atccgggcaa ctacctcgag cagcacctgc gcga
//
LOCUS       DAOQXS010000352         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30188_length_3325_cov_3.198165, whole genome shotgun sequence.
ACCESSION   DAOQXS010000352 DAOQXS010000000
VERSION     DAOQXS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30188_length_3325_cov_3.198165"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2644
                     /gene="uvrA"
                     /locus_tag="PKM34_09515"
     CDS             <1..2644
                     /gene="uvrA"
                     /locus_tag="PKM34_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ83869.1"
                     /translation="RRYVESLSAYARQFLGRMSKPRVEYIKGISPAIAIEQKVNTRNP
                     RSTVATSTEIYDYIKLLFARTGKTYSPVSGKLVKRHTVTDVTDFIMAQEEERRVTITC
                     PLIRKADRDMKTELTLLLQQGFNRLRINGDFIRIEDVIHSGIPGKEKDIEILIDRFSV
                     NRSDEDLAGRVADSVQTAFYEGHGICTVRVEVNGQETLIEFSNRFERDGIVFEEPSVN
                     LFSFNNPYGACKRCEGFGSIIGIDEDLVVPNKGLSVYEDAIACWKGEKMGEWKEQLVM
                     HAYKFDFPIHKPFYQLTDEQRLLLWTGNQYFQGLNEFFRFVEEQAYKIQYRVMLSRYR
                     GKTVCPECKGTRLRKDANYVKVGGKSVSEILLLPVSQALAFFKQVNLSDHDQKVSQRL
                     LTEIKTRLQFLEDVGLGYLSLNRQSSTLSGGESQRINLATSLGSSLVGSMYILDEPSI
                     GLHPRDTHRLINVLMNLRNLGNSVIVVEHDEEIIRASDYLIDIGPLAGSQGGEVVFAG
                     TFGQMQEDNKSLTSKYISGEMTIPVPKSRKPWKEYIEIMGARENNLKGIDVKFPLNVL
                     TVITGVSGSGKSSLVRNILYPALKKIYGGSSEKTGNYGRLEGDYNRIGQVEFVDQNPI
                     GRSSRSNPATYLKAWDDIRNLYSDLQLAKVRGYKPGYFSFNIPGGRCEECEGEGVVKI
                     EMQFMADLSLTCESCEGKRFKDEVLDVRFQDRNIVDILEMTVEQAIAFFSSSQTHIST
                     CRRIVEKLQPLSDVGLGYLQLGQSSSTLSGGEAQRIKLAYFLTKGGTSSPTLFIFDEP
                     TTGLHFHDISKLLDAFEALISKRHSVIVIEHNPEVIKCADWLIDLGPEGGDAGGHVVF
                     AGVPEGLVNVERSFTGKYLKEKIK"
     gene            complement(2708..>3325)
                     /gene="rlmB"
                     /locus_tag="PKM34_09520"
     CDS             complement(2708..>3325)
                     /gene="rlmB"
                     /locus_tag="PKM34_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNQ83870.1"
                     /translation="QLAKTYQIPFQFVPQEKLDRITRKNHQGVIAMISQISYGSLENL
                     VQMVFEQGKTPLFLILDGITDVRNFGAISRTAECAGVDGIIIPMQGSAQINEEAVRTS
                     AGALMRIPVCREKSLKSTAAFLRESGITLFAATEHGKEPYFSGDYSGPAAIIMGSEET
                     GISPELLRICDKMVQIPMQGEISSLNVSVAAGVLLFEVVRQRMGS"
BASE COUNT          856 a          822 c          881 g          766 t
ORIGIN      
        1 gaggcggtat gtggaaagcc tgagcgccta tgcacggcag ttcctgggaa ggatgagcaa
       61 accccgggtc gaatatataa aaggtatatc gccggctatc gctatcgaac agaaggtcaa
      121 tacgcgcaat ccgcgttcga cagtcgctac atcgaccgaa atctacgatt atatcaaatt
      181 gctgttcgcc cgcaccggta agacctactc gcctgtctcg ggcaagctgg tcaagaggca
      241 tacggtaacg gatgtcaccg attttatcat ggcgcaggaa gaagagcgcc gggtaaccat
      301 cacctgcccc ctgatcagga aagccgaccg ggatatgaaa accgaactca ctttgctcct
      361 gcagcaaggt ttcaaccgcc tgagaatcaa cggtgatttc atccggattg aagatgtgat
      421 ccattccggc attcctggca aggagaagga tattgagatc ctcatcgacc ggttttctgt
      481 taaccggtcc gacgaagatc tggccggccg ggtggctgat tctgttcaaa cggcatttta
      541 tgaaggccat ggcatatgta ctgtcagggt tgaggttaat ggccaggaga cccttattga
      601 attttccaac cggtttgagc gggacgggat cgtatttgaa gagccttccg ttaacctgtt
      661 cagcttcaat aatccttacg gcgcatgcaa acgctgcgaa ggtttcggca gcatcatcgg
      721 catcgatgaa gatcttgtcg taccaaacaa aggattgtct gtatatgagg atgccatagc
      781 ctgctggaaa ggggaaaaga tgggcgaatg gaaagaacag ctggtgatgc atgcctataa
      841 attcgacttc cccatccaca aaccctttta tcagctgacc gatgaacagc gcctcctgct
      901 ctggaccgga aatcagtatt tccaggggct gaacgagttt ttcaggtttg tggaagaaca
      961 ggcttataaa atccagtacc gggtgatgct gtcgaggtac cgcggcaaga ccgtttgccc
     1021 cgaatgcaaa gggacgcggc tccggaaaga tgcgaattat gtaaaagtcg gcggaaaatc
     1081 ggtgagcgag atcctgcttt tgcctgtttc ccaggcgctg gcatttttca aacaggtaaa
     1141 tttatcggat catgatcaga aagtttccca gcgcttgctg acagagataa aaacccgcct
     1201 gcaatttctc gaagatgtag gcctgggcta tctatcgctg aaccgccagt catcaacgct
     1261 atcgggagga gaatcccagc ggatcaacct ggccacttcg ctgggcagca gcctggtcgg
     1321 atcgatgtat atcctcgacg agcccagtat cggactgcat ccccgcgata cgcaccgact
     1381 gatcaatgtg ctgatgaacc tgagaaacct gggcaattcg gtgatcgttg ttgaacacga
     1441 cgaagaaatc atcagagctt ctgattatct gatcgatatc gggccgctgg ccggcagcca
     1501 gggcggtgaa gtcgtgtttg cagggacttt cggacaaatg caggaagaca ataaaagcct
     1561 cacctctaag tatatttcag gagaaatgac cattcccgtt ccgaaaagca ggaagccgtg
     1621 gaaggaatat attgaaatca tgggcgcccg tgaaaacaac ctgaaaggga tcgatgtgaa
     1681 gttcccattg aatgtactga cggttataac cggggtcagc ggatcgggca agtcgtcgct
     1741 ggtgcgtaat atcctgtacc cggccctgaa aaagatctat ggcggaagca gcgagaaaac
     1801 agggaactat ggacgactgg aaggcgacta caaccggatc ggacaggtgg agtttgtcga
     1861 ccagaacccg atcggccgct cttcacgttc taatccggct acatatctga aagcctggga
     1921 cgatatccgt aatttatatt ccgacctgca attggcaaaa gtcaggggat ataaaccggg
     1981 ttatttttca ttcaatattc cgggtggaag atgtgaagaa tgtgaaggcg aaggggtggt
     2041 gaagatcgag atgcagttca tggccgacct gagccttacc tgtgaatcat gcgaaggaaa
     2101 aaggttcaag gatgaggtcc tggatgtgcg gttccaggat agaaacatcg tcgatatcct
     2161 ggaaatgact gttgagcagg ccattgcgtt cttttcatct tcacaaactc acatttccac
     2221 ctgccggcgg atcgttgaaa agcttcaacc gctaagcgat gtgggcctgg gctatctcca
     2281 gctcggccaa tcgtcgagca ccttaagcgg tggcgaagcg cagcgtatca aactggccta
     2341 ttttctgacc aaaggcggaa cttcatcccc taccctgttc atcttcgatg aacccacaac
     2401 cggcctgcac ttccacgata tcagcaaact cctcgacgct ttcgaagccc tcattagcaa
     2461 aaggcactcg gtgatcgtga tcgagcataa cccggaggtg atcaaatgcg ccgactggct
     2521 gatcgacctg ggcccggagg gaggcgatgc cggaggacac gttgtttttg ccggcgttcc
     2581 cgaaggcctt gttaatgttg agcgttcgtt taccggaaag tacctgaaag aaaaaatcaa
     2641 atgaattcag tccacaatct tcaaataaga tccaagattg taaactgtaa actgtaaacc
     2701 gtaaatccta cgatcccatc ctttgcctta ccacctcgaa cagaaggaca ccggcggcta
     2761 cagagacatt caatgatgat atctcgccct gcatgggtat ctgtaccatt ttatcacaaa
     2821 tccgcaataa ttcgggtgaa atgccggtct cttccgagcc catgatgatc gcagccggac
     2881 cggaataatc gcccgaaaaa taaggttctt taccgtgttc ggtggcggca aagagcgtga
     2941 tcccgctctc cctgagaaaa gcagcggttg atttcaacga cttttcacgg cataccggaa
     3001 tgcgcatcag tgcgccggcg ctggtcctga ccgcttcctc gttgatctgg gcgctgccct
     3061 gcatcgggat aatgatgcca tcgacacctg cgcactcggc cgtgcggctt atggcgccga
     3121 agttgcgaac atccgtaatt ccgtcgagga tcaggaacag aggtgttttt ccctgctcga
     3181 acaccatttg caccaggttt tccagtgagc catagctgat ctgcgagatc atggcgatca
     3241 ctccctggtg atttttccgg gtaatgcgat cgagcttttc ctgcgggacg aactggaaag
     3301 gtatctggta cgttttggca agttg
//
LOCUS       DAOQXS010000353         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30374_length_3310_cov_2.699539, whole genome shotgun sequence.
ACCESSION   DAOQXS010000353 DAOQXS010000000
VERSION     DAOQXS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30374_length_3310_cov_2.699539"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKM34_09525"
     CDS             complement(<1..1253)
                     /locus_tag="PKM34_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNQ83871.1"
                     /translation="MKNISLLLISVLFVLNFNAQNWEFFTPENTGLPSDHVFSVATDS
                     MGTMWFGTRSGLSRFDGTTWTNYNILNSGLPSDMIYAITIDKFGNKWLGTTGGIVKYN
                     GADWIVYNKSNSGLPCDTISTIAIDHLNNKWFGITPKYEMNQGYIRGGILCFNDTTWT
                     LYNSENSLLPSNYITALAIEESGAKWIGTYAGLAKLDGSEWTLLNAENSDLPSDSISS
                     ISIDKYNHKWIGFSFTNQESGHCFGGDGIAKFNDLTWQFYTKENSGLPSNNIYSIFID
                     EFENKWIGTTAGLVLYDDETWAVFNSSNTAMPDDRVNAVCIDTNGNNWISILTDNWAG
                     YYLGAGIAQFDGSGWTLYDHANTGLPYYWISSVAIDHSGNKWVGTYKNGLSSFDGSKW
                     TNYYRSNSGLPSDTINKILIDSLGN"
     gene            complement(1265..2182)
                     /locus_tag="PKM34_09530"
     CDS             complement(1265..2182)
                     /locus_tag="PKM34_09530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83872.1"
                     /translation="MNEIEFSVVVPVFNSEASLEELFHRLTEVLKATGKPYEMIFVDD
                     GSGDRSWEMLKKIKAENPDGFTAIRLAKNFGQHSATFCGFSFAKGKHIITLDDDLQCP
                     PEEIPKLIRTMEETDAELVYGIYRKKRHSAARNISSRSFKSLSRLFGSQTEGSSFRLI
                     SGSIIQKILGHHQNFVFIDEILHWYTDFIGYATVEHHPRKYKRSGYSRGKIWRFFTEV
                     MYHYSTAPLKVMVYGGMVSSVIFFIFSIYFILRKILMNVPLGYTSLIVAILFSTSLIL
                     LSLGVIGEYLSRIYMVQNKKPLYSVKKVL"
     gene            2416..>3310
                     /locus_tag="PKM34_09535"
     CDS             2416..>3310
                     /locus_tag="PKM34_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83873.1"
                     /translation="MKTTKILMCIFVMLVFIGLNTTKGQWLYNGTSIYNSNSGNVGIG
                     NNSPGSLLYVAKNMTEPTITVRNLGGTGGAAYTMTDDASGANWKFKATNNGGFKIRDH
                     ANLLDVITIEPNSTANAIYINAEGNLGVRTSTPAANFHVAENNPSYTAAFGNMISGWS
                     DGTNVSIGNDDFNSILYVGQDMSHKGYLIWNHHPEPDAATFMIGTYDGTNPMILQPAG
                     GNVGIGTSTPYARLDVMNNLYDYSRIGNTATVANEFLNYEETATGDGQCAIMTVRHRA
                     EQNDGAAYNIGQTNASIIGYNV"
BASE COUNT          933 a          731 c          728 g          918 t
ORIGIN      
        1 atatttccaa ggctgtcgat taggatttta tttatcgtat cggaaggtaa acccgaattt
       61 gacctgtagt aatttgtcca tttacttcca tcgaaactgg aaagtccgtt tttataggtt
      121 ccgacccatt tattaccgga atgatcaatg gctactgagc taatccaata gtaaggcagg
      181 ccggtatttg catgatcgta taaagtccag ccggagccat cgaattgagc gatacccgcc
      241 ccaagatagt acccagccca attgtctgtc agaatactga tccagttatt gccattcgtg
      301 tcaatacaaa cagcatttac tctatcatcg ggcattgcag tattggaact gttaaacact
      361 gcccaggtct cgtcatcgta taggaccagc cccgctgttg tgccgatcca tttattttca
      421 aattcatcaa tgaatattga atagatatta tttgagggta aacctgaatt ttcttttgta
      481 taaaattgcc aggtaagatc attaaatttt gctattccat ctccgccaaa acaatgtccg
      541 gattcttgat tggtaaatga gaatccaatc catttatggt tgtacttatc gatcgatatg
      601 gaacttatcg aatctgaggg cagatcagag ttctccgcat tcaggagcgt ccattcggaa
      661 ccatccagct ttgctaatcc ggcatatgta ccgatccatt tagcgccgga ttcttcaatg
      721 gcaagagcag tgatatagtt ggagggtaat aacgaatttt cggaattata cagggtccag
      781 gttgtatcat taaaacacag gatcccgcct ctaatgtacc cctggttcat ttcatatttt
      841 ggtgtaatgc caaaccattt attgttcaga tgatcgattg caattgttga aatcgtatca
      901 catggcaggc ctgaattcga tttattatac acaatccagt cggccccgtt atatttcacg
      961 atcccgcccg tagtgccaag ccatttgttt ccaaatttgt caatggttat ggcataaatc
     1021 atatccgatg gtaacccgga gttcaaaata ttataattgg tccaggttgt tccgtcaaat
     1081 cgtgaaagtc ccgaccttgt gccaaaccac atagttccca tgctatcggt tgccacagaa
     1141 aatacatgat cggaaggcaa gcccgtattt tcaggtgtga aaaactccca attctgtgca
     1201 ttaaaattta aaacaaataa tacactgata aggagaagcg aaatgttttt catttcagtg
     1261 atatttacag caccttcttt accgaataca gcggcttctt gttctgaacc atatatatcc
     1321 ggctgagata ttccccgata accccgaggg aaagcaggat caggctggtg gagaatagga
     1381 tggcaacgat cagcgaggtg tatcccagcg gaacgttcat caggatcttc ctcaggataa
     1441 agtagatgga gaagataaaa aagatgacag atgataccat tccgccgtac accatcacct
     1501 tcagcggggc agtggaataa tggtacatca cctcggtgaa gaaccgccag atcttgcccc
     1561 ggctgtaccc cgaccgtttg tatttccgtg gatggtgctc cacggtggcg taaccgatga
     1621 aatcggtata ccagtgcaga atttcgtcga tgaaaacgaa attttgatgg tgtcccagga
     1681 ttttttgtat gatgctgccg gagatcagcc ggaatgatga tccctccgtc tggcttccga
     1741 acagccgtga gaggctcttg aacgaccggc tgctgatgtt cctggccgcc gaatgccgct
     1801 tcttccggta gatgccgtaa accagctcgg catcagtctc ttccatcgta cggatcagct
     1861 tgggtatctc ttcgggcggg cattgcaggt catcgtcgag ggtgatgata tgttttcctt
     1921 tggcaaagct gaaaccgcaa aatgtggcgc tgtgctgccc gaagttcttc gccagccgta
     1981 tcgccgtaaa tccatcgggg ttctccgcct tgatcttttt cagcatctcc cagctccggt
     2041 cgccgcttcc gtcatccacg aagatcattt catacggctt gcccgtcgcc ttcagaacct
     2101 ccgtcaggcg atggaaaagt tcctccagcg acgcttcgct gttgaaaacc gggactacga
     2161 ccgaaaattc gatttcattc acgcggatga tttttcacaa aggtagcaaa gagatgaaat
     2221 aattaaaaat tgaaatttga aaattgaaat ttaaaggaaa atccttgata agtaggaaaa
     2281 ttcgtaatac ctttcggcaa ctattactcc tctctgaaat cactaataaa agatgatcgg
     2341 ggatcatctg cagaccaaac ctgtaatctc tcatgttata aatactaatt attgattatc
     2401 attaaattaa agattatgaa aacaaccaaa atcttgatgt gcatcttcgt gatgcttgtt
     2461 tttattggtc ttaacacaac aaaaggccag tggctctaca acgggaccag tatttacaat
     2521 tctaactcag gcaatgtggg tattgggaac aattcaccgg gatcactgct ttatgttgca
     2581 aagaacatga ctgaacctac gatcactgtt cgtaatcttg gcggcaccgg tggcgctgcg
     2641 tataccatga ctgatgatgc cagcggggcg aattggaagt tcaaagcaac gaacaacggg
     2701 ggctttaaga tccgtgacca tgcaaatctg cttgatgtga taaccattga gcccaacagc
     2761 acggcaaatg cgatctatat caatgccgaa ggcaacctgg gcgtgagaac ttcaactccg
     2821 gctgccaatt ttcatgttgc agaaaataat ccaagctaca cggctgcttt tggaaatatg
     2881 atcagtggct ggagtgacgg tactaatgtg agcattggga atgatgattt taattcaatc
     2941 ttatatgtcg gccaggatat gagtcataaa ggatatctta tctggaacca tcatcccgaa
     3001 ccagacgctg ctactttcat gattggtact tatgatggga ctaatccaat gatactccaa
     3061 cctgcaggcg gaaatgtagg aattggcaca tctacgcctt atgcccgctt agatgtcatg
     3121 aacaatttat atgattacag cagaattggg aatactgcga ctgtcgcaaa tgagtttctt
     3181 aattatgagg aaacggccac aggggatggc caatgcgcaa ttatgacggt gaggcatcgt
     3241 gctgaacaaa atgatggggc cgcctataat ataggtcaaa ccaatgcgag tataataggt
     3301 tataacgttt
//
LOCUS       DAOQXS010000354         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30526_length_3298_cov_3.853839, whole genome shotgun sequence.
ACCESSION   DAOQXS010000354 DAOQXS010000000
VERSION     DAOQXS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30526_length_3298_cov_3.853839"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKM34_09540"
     CDS             complement(<1..488)
                     /locus_tag="PKM34_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ83874.1"
                     /translation="MTIGEKSVVSMTYTLREESSAGQVIQKVTEDRPFVYLFGVAGLL
                     PAFKANLEGKTSGEEFAFTLSNEDAYGLPSDENILSLDKKIFEIDGMFDEANIKVGEV
                     VPMEDENGYPLSGKILEIGTDSVLVDFNHPLAGMNLFFEGKILEVREATDEEMAHGHV
                     HGP"
     gene            612..1508
                     /locus_tag="PKM34_09545"
     CDS             612..1508
                     /locus_tag="PKM34_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ83875.1"
                     /translation="MKDIVLSINGLTKRYGSITAVSQLDLEIERGSVFGILGPNGSGK
                     TTTLGVLLDVTRPDSGSFSWFGGLPEKTQRKRIGAILEVPLFYPYLSGKQNLELVAKI
                     KDLPGDDVEEALQTVGLYDRMHSKFRTYSLGMKQRLALASTLIGKPEVLILDEPTNGL
                     DPRGIAEIRNIILNVAAQGVTIILASHILDEVQKTCSHVAVLEKGKKLFSGPVAQVLN
                     EAHWVELAAENMAGLQLALQEAEFIENLQEEGDRYIIKLKSDLSQADLNRYFFGKGIV
                     LSHLAIRKKSLEKYFLELLDKS"
     gene            1505..2323
                     /locus_tag="PKM34_09550"
     CDS             1505..2323
                     /locus_tag="PKM34_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ83876.1"
                     /translation="MMRLLQIELRKILPYKMFWVMCGIYLVSLLFFSWGLPSLIDYFT
                     MKSNVPEIKLLTKFLYNFPDIWQNLSWAASLRFFIKIFIGIIMIILVTNEYSYLTIRH
                     NVFNGLSRTDFLMSKVYVAILISVAATLLILLTGITLGLFYSSTISVQAFFGKMAFLA
                     GYFIELLAFLLFSLMVGILIKRTGFAIGVLFVYPILELIIQQKVPESVQPFLPLNAIN
                     HILRTPNTSLIQFSSPEFDIELQTALHAQDILVALAYAVLFVLVSLWVIRKRDL"
     gene            2522..2761
                     /locus_tag="PKM34_09555"
     CDS             2522..2761
                     /locus_tag="PKM34_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83877.1"
                     /translation="MKTLSLKLDDNVFRETEQVTGYLKKSRNRYINEALDHFNKVHQR
                     KILADRLEMESKLVAKESADVMNEFEAFEDESQAI"
     gene            2742..3083
                     /locus_tag="PKM34_09560"
     CDS             2742..3083
                     /locus_tag="PKM34_09560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ83878.1"
                     /translation="MKARQYEIWIADLNPRIGTEPGKVRPVVIVQTDLLNKHHPSTLI
                     CPVTTSVQPESEILRVHLAKGSSGLDEACDVMMDQVRAIDNARLLKKIGKLPSEAILK
                     VKENLSIVLDL"
BASE COUNT          853 a          761 c          770 g          914 t
ORIGIN      
        1 ggaccgtgca catggccatg ggccatctct tcatcggtag cttcgcgtac ttccaggatc
       61 ttcccttcaa aaaagaggtt catgccggcg agcggatggt tgaaatcgac taatacagaa
      121 tcggtgccga tctcgaggat cttacccgac agcgggtatc cgttctcatc ttccatcggt
      181 accacctccc ctactttaat gtttgcctcg tcgaacattc catcaatctc gaaaatcttc
      241 ttatccaggc tgaggatgtt ttcatcggaa ggaagcccgt aagcatcttc attacttaaa
      301 gtaaaagcaa attcttcacc ggaggttttt ccctccaggt tggctttaaa agccggaagg
      361 agtccggcca cgccgaacag gtagacaaat ggcctgtcct ccgtaacttt ctgaatcacc
      421 tgccccgctg agctttcttc acgcaatgtg taggtcatcg aaacgaccga tttttcacca
      481 attgtcatac cgtttggaat tttaagttat ggcggcaaag ataacaaagt ttgggtggaa
      541 aattattccg ttccgttgta atgaaatcaa tgcagtatcg ttttatattt gtttaacgca
      601 aatcaaaaca tttgaaagat atcgtccttt ccatcaatgg cctgaccaaa cgatacggat
      661 cgatcaccgc ggtcagccaa ctggacctgg aaattgagcg gggatctgtt ttcggcatcc
      721 tcgggcccaa cggaagcggt aagacaacaa ccctcggcgt gcttctcgat gtcacgcggc
      781 ccgactcggg ctcgttctcc tggttcgggg gactgccaga aaaaacacag cgaaagcgga
      841 tcggcgccat cctggaagtc cctctttttt atccttacct gagcggaaag caaaacctgg
      901 aactcgtggc gaagatcaaa gaccttccgg gcgacgatgt ggaggaagcg cttcagacgg
      961 tagggctgta cgaccggatg cacagcaagt tccggactta cagcctgggg atgaagcaac
     1021 gactggcgct ggcctcgaca ctgatcggca aacccgaagt actgatcctg gatgaaccca
     1081 ccaacgggct tgatcccagg ggaatagccg agatcaggaa cattatcctg aatgtagcgg
     1141 cacaaggtgt aacgatcatc ctggccagcc acatcctcga cgaggtgcag aaaacctgca
     1201 gccatgtcgc ggtcctggag aaaggcaaaa agttgttctc agggcctgtg gcgcaggtgc
     1261 tgaatgaagc ccactgggtg gaactggctg cggaaaatat ggccggtctg caattggcgc
     1321 tgcaagaggc agaattcatc gaaaacctgc aggaagaagg cgaccggtac atcatcaagc
     1381 tgaaatccga cctctcacaa gccgacctga accggtattt cttcggcaaa ggaatcgtgt
     1441 tatcgcacct ggcgattcgt aaaaaatctc ttgaaaaata ttttctcgaa ttattggaca
     1501 aatcatgatg agacttcttc agatcgaatt acggaaaata ttgccctaca agatgttctg
     1561 ggtaatgtgc gggatttacc tggtgtcgtt attattcttt tcctggggac tgccgagcct
     1621 gatcgactac ttcaccatga aatcgaatgt ccccgagatc aagctcctga ccaaattcct
     1681 gtataatttc cctgatatct ggcagaacct gtcgtgggcg gcctcgctcc ggttcttcat
     1741 caagattttt atcgggatca tcatgatcat cctggtcacc aatgaatatt cctacctgac
     1801 gatccggcat aatgtattca atggcctgag ccggaccgac ttcctgatgt cgaaggtata
     1861 tgtggccatt ttgatcagtg tggcggccac cttgctgatt ttactcaccg gaatcacatt
     1921 aggattgttt tattcttcga ccattagtgt ccaggcattt tttggcaaga tggcattttt
     1981 agccggctat ttcattgaat tgctggcatt cttactgttt tcgctgatgg tcggcatttt
     2041 gatcaagcgg accggttttg ccatcggtgt gcttttcgtt tatccgatcc tggagctcat
     2101 catccagcag aaggtgccgg agagcgtgca gccgttcctg ccgctgaatg ccatcaatca
     2161 cattctccgc acacccaaca cctcgctgat ccagttcagt tctcccgagt tcgacattga
     2221 gctgcagacg gcattgcatg cacaggatat acttgttgcc ctggcatatg ccgttttatt
     2281 cgtgctggtt tcgttgtggg tcatacggaa acgcgacctt tgaaatgttg aatgttgaat
     2341 gttgaatttt aaattcgggt tattttatta actgcttaga attctgcact taacacttct
     2401 ttgcgaacct ttgtttcact ttgcgcaact ttgcgtaaca gcttttgtgt agtttatgtt
     2461 gtaaaagaaa ttggttcaat tccggaaagt atatactttt gcaatatact gataacatat
     2521 catgaaaaca ctttctttaa aattagatga caatgtattc cgggagaccg agcaggtaac
     2581 aggatatttg aaaaaatcgc gtaaccgtta tattaacgag gctttggatc atttcaacaa
     2641 ggtccatcag cggaaaatac ttgctgaccg gctggaaatg gaatcgaagc ttgtcgcaaa
     2701 ggaatcggca gatgtgatga atgaatttga ggcgtttgaa gatgaaagcc aggcaatatg
     2761 agatctggat tgccgatctg aatccgcgga ttggaaccga gccgggaaag gtgcgtcctg
     2821 ttgttattgt tcagactgat ttactgaata agcatcatcc ttcaacgctt atttgtccgg
     2881 ttactacgag tgttcagccg gagagcgaga tattgcgggt tcatttagcc aaaggcagta
     2941 gtggacttga cgaagcctgt gatgtgatga tggaccaggt tagggcgatt gacaatgcaa
     3001 ggttattaaa aaaaattgga aagctgcctt cagaagcgat tctaaaagtg aaagaaaatc
     3061 tatcaattgt gcttgattta taataagata aactaatctc ttccttttca ttattcacta
     3121 ttcactattc gtttttcact tttttccatt cctaccgggt attttttcta ttttccggat
     3181 taagctattt gcaagattgt ttttcaccac atagacacca cctgtcctga aacctcagga
     3241 tagtgaatat tcctgcccaa actgtaccag tgattcctga gcaaagtgta ccagtaat
//
LOCUS       DAOQXS010000355         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30625_length_3291_cov_4.157293, whole genome shotgun sequence.
ACCESSION   DAOQXS010000355 DAOQXS010000000
VERSION     DAOQXS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30625_length_3291_cov_4.157293"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKM34_09565"
     CDS             <1..1300
                     /locus_tag="PKM34_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNQ83879.1"
                     /translation="WEAPVLVQRLCGICPVSHHLAAAKALDVIVGAGTGEGLTPTGEK
                     MRRLMHYGQMFQSHSLHFFHLVSPDLLLGIDADPAIRNVIGVAIHHRDLAVQGVMMRK
                     FGQEIIAATAGKKIHGTGAIPGGINKHLTIEERDKFLKGADPLNIDKMIEWSIGALDF
                     FKSYYKKNKEFIDSFAYFPSNHLSLVRKDGALDLYHGVLRAVDTDGKKILNDIDYQYY
                     ADYIEEEVRSWSYMKFPYLKELGPQKGWYRVGPLARLNTCDFIPTPLAQKEFEEFKSL
                     TNGKPNNHCMHYHYARLIEILHSAEEIKKLLNDPDLLEGPLVAKGQKLNEGVGLIEAP
                     RGTLFHHYRINEFDQITMANLIVSTTNNNEPMNQSVNRVAKSWMNGKTEITEPMMNAV
                     EVAIRAYDPCLSCATHALGQMPLDISLYDKDNNLIDRKRT"
     gene            1303..1782
                     /locus_tag="PKM34_09570"
     CDS             1303..1782
                     /locus_tag="PKM34_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNQ83880.1"
                     /translation="MAEFFNILIYGYGNPGREDDGLGIELVKQLEQWAAENELQGISF
                     DSNYQLNIEDAEAISDMDVVIFADASTEDIEDFILTKVTGETEVSFTTHAASPGYIVK
                     LCEEIFGKSPEVYLLHIKGYQWDFYEGLSDNAVKNLENALNFISNFILEKLKQNSKP"
     gene            1796..2230
                     /locus_tag="PKM34_09575"
     CDS             1796..2230
                     /locus_tag="PKM34_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83881.1"
                     /translation="MDLSTSYMGLKLRNPLIVSSSRITSTVDDVKKCADNGAGAIVLK
                     SLFEEQFLADSNRLMDQDQKYFWFPEAIDFINQHSREHGIKGYLKLIKGAKANTDVPI
                     IASVNCVTAQEWPRYSKELVDAGADGIELNVFIVPKNEHDKR"
     gene            2217..2771
                     /locus_tag="PKM34_09580"
     CDS             2217..2771
                     /locus_tag="PKM34_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNQ83882.1"
                     /translation="MTSAEIEDLYVQIVSEVRRNVNVPIAVKIGPFFSNGFSMVKRLN
                     DAGANAIVVFNRFYRPDIDIDNECITRPNYLSCPQEMGQPLRWVSLFSSRVNIDMAGN
                     TGIHEGSGMIKMLLAGAAAVQVCSTLYINGLGYIDTMIGDLKKWMAWKGYEKIDDFKG
                     KLTKRHENIAAFERVQFLKQALGE"
     gene            complement(2778..>3291)
                     /locus_tag="PKM34_09585"
     CDS             complement(2778..>3291)
                     /locus_tag="PKM34_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83883.1"
                     /translation="NSLFLKGASFEECTFFQNAYFRGSAYHEEARFQNAVFMQKANFL
                     NAEFDVTASFQSATFNSEAQFSSAKFLGYADFGLTRFNGNFFSNYATFGKQVIFNHAT
                     FYGQADMTSMTLKRSEMKNCRFFNEARFAKSIVEESLTLSNTFFLFGKPDLSSFDQQK
                     VIQDGLRTGE"
BASE COUNT          926 a          753 c          789 g          823 t
ORIGIN      
        1 ctgggaagcc ccggtgctgg ttcagcgact ctgcggtatc tgccccgtaa gccatcacct
       61 ggccgctgcc aaagcactcg atgtgatcgt cggagccggc accggcgaag gacttacccc
      121 gaccggcgag aagatgcgcc gcctgatgca ctacgggcag atgttccaat cgcattcatt
      181 gcatttcttc cacctggttt cccccgacct gctgctgggt attgacgccg atccggctat
      241 ccggaatgtc atcggcgtgg ccattcatca cagagatctg gccgtacagg gagtgatgat
      301 gcgtaaattc ggacaggaga tcatcgctgc tacagccgga aagaaaatcc atggtaccgg
      361 agccatcccc ggaggaatta acaaacacct gaccattgaa gagcgggata aattccttaa
      421 aggagccgat cctctcaata tcgataagat gatcgaatgg tcaatcggag ccctcgactt
      481 ctttaaatcc tactataaaa agaacaagga attcatcgat tcattcgcct atttcccgtc
      541 gaaccacctg agccttgtcc ggaaagacgg agccctcgat ctctatcatg gtgttttacg
      601 tgccgtggat acggatggaa agaaaatcct taatgatatt gattatcagt attatgctga
      661 ttatattgaa gaagaggtca ggtcctggag ttacatgaag ttcccttatc tgaaggagct
      721 tggcccgcaa aaaggatggt acagggtagg acccctcgct cgcctgaaca cctgtgactt
      781 tattccgaca cctctggctc agaaggaatt tgaagaattc aaatcgctga ccaacggtaa
      841 accgaacaac cattgcatgc attatcatta tgcacgcctg atcgaaatcc tgcattctgc
      901 cgaggaaatc aagaaattgc tcaacgatcc cgacctgctg gaagggccgc tggttgcaaa
      961 aggccagaag ctgaacgaag gggtgggcct catcgaagct ccccgcggaa cactcttcca
     1021 tcattaccgg atcaatgaat tcgaccagat caccatggct aacctgatcg tttccacaac
     1081 caacaacaac gagccgatga accagtcggt caatcgcgtt gccaaatcgt ggatgaacgg
     1141 aaagaccgag atcaccgagc cgatgatgaa tgcggtggaa gtagctatcc gcgcttatga
     1201 cccgtgcctg agctgcgcaa cgcatgccct gggccagatg ccactggata tttcgctgta
     1261 tgacaaggat aataacctga tcgacaggaa acggacataa ttttggcaga gttctttaac
     1321 attctcatat acggatacgg aaatcccgga cgggaagacg acgggctggg tattgaactg
     1381 gtaaagcaac ttgaacaatg ggctgctgag aatgaactgc aaggaatttc tttcgattcc
     1441 aactaccagt tgaatattga agatgctgaa gctatttccg atatggatgt cgttattttt
     1501 gccgacgctt ctacagaaga tattgaggat ttcatcctga cgaaggtaac gggggagact
     1561 gaagtttcat ttacgacaca cgcagcctct cccggctaca ttgtcaagct ttgcgaggaa
     1621 atcttcggaa aatccccgga agtttatctt ttgcatatta aagggtacca atgggatttc
     1681 tatgaaggat tatccgataa tgccgttaaa aaccttgaaa atgcgttgaa ttttatcagt
     1741 aattttatat tagaaaaact taaacagaat agcaaacctt aaaacctctc taattatgga
     1801 tttgtcaaca tcctatatgg gattaaagct gcggaatccc ttaattgtca gcagttcacg
     1861 aattaccagc acagtcgatg atgtcaaaaa atgtgctgac aacggagcag gtgctatcgt
     1921 cttgaagtcc ttgtttgagg aacaatttct tgctgattcc aaccgcttga tggaccagga
     1981 tcagaagtac ttctggtttc cggaagccat tgattttatc aatcagcatt ccagggaaca
     2041 tgggatcaaa ggatacctga aactgatcaa aggagccaaa gccaatactg atgttccgat
     2101 cattgccagt gtgaactgtg ttacggcaca ggagtggccg cgctattcga aagaactggt
     2161 tgacgcagga gcagatggca ttgaactcaa tgttttcatc gttccgaaaa acgagcatga
     2221 caagcgctga aattgaagac ctttatgtac agatcgtatc ggaagtccgg cgtaatgtca
     2281 atgttccgat tgcggtgaag atcggaccat tcttcagtaa tggtttcagc atggtcaaaa
     2341 ggttaaacga tgcgggtgcc aatgcaatag tcgtctttaa ccgtttttac cggcctgata
     2401 ttgatattga taatgaatgt attaccagac caaattacct gagctgccca caggaaatgg
     2461 gccagccttt gcgttgggta tccctgtttt ccagccgtgt taatattgat atggcaggta
     2521 atacgggaat acacgaagga tcgggaatga tcaaaatgct gctggccggc gcagccgccg
     2581 tccaggtttg ttcaaccttg tatatcaacg gtctgggcta tatcgatacg atgatcggag
     2641 accttaaaaa atggatggcc tggaaaggat acgaaaaaat cgatgatttt aaaggaaaac
     2701 tgaccaaacg ccatgaaaat attgcagcgt tcgaacgtgt ccagttcctg aaacaggccc
     2761 tcggagaata agcggcttta ctcgccggtt ctcaggccat cctgaataac tttttgctga
     2821 tcgaaactgc tcaggtcagg ttttccgaat aaaaagaagg tattggaaag cgtcaggctt
     2881 tcttcaacaa ttgatttggc aaacctggct tcgttgaaaa acctgcagtt cttcatctca
     2941 cttctcttca gggtcattga tgtcatatca gcctggccgt aaaaggtggc atggttgaaa
     3001 ataacctgtt tgccgaaagt ggcgtaattg gagaagaaat ttccgttgaa ccgggtcagg
     3061 ccgaaatcgg cgtatcccag gaattttgcg gaactgaact gcgcttcact gttgaatgtc
     3121 gcgctttgaa aactggcggt cacatcaaat tccgcattca ggaaattggc cttctgcata
     3181 aagactgcat tctggaaacg ggcctcttca tgatatgcgc tccccctgaa ataagcattc
     3241 tgaaaaaatg tgcattcctc aaagctggcc ccttttaaga agagggaatt c
//
LOCUS       DAOQXS010000356         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30646_length_3290_cov_3.377743, whole genome shotgun sequence.
ACCESSION   DAOQXS010000356 DAOQXS010000000
VERSION     DAOQXS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30646_length_3290_cov_3.377743"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PKM34_09590"
     CDS             <1..700
                     /locus_tag="PKM34_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83884.1"
                     /translation="QSCKNIEYIAIDGGSTDGTVEFLRSLGDCVRWVSEPDRGQSDAV
                     NKGMQMATGDIIGWLNSDDLYLPGALQTVVRHFHANPDCQWAYGRCIIIDKEGVERWK
                     YITRYKNIRLRKFTLDKLYRENFISQPAVFFRKDLWNQAGGLDLSLHYAIDYDLWLRF
                     ALRSPALVIDSDLAAFRRHGASKSETGFRDQFYEQYAVAKRYHPSGFNRLIHRFNIFK
                     IITSYRIIGLFFRF"
     gene            complement(614..1066)
                     /locus_tag="PKM34_09595"
     CDS             complement(614..1066)
                     /locus_tag="PKM34_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNQ83885.1"
                     /translation="MNESLGSIGKNIQTTQVIAILVSVGLFLFILYLVRKKKIKEEYS
                     LLWLFSSVVFIVFSIWREGLEYFAGLMGIAYPPAALFLILLLAIFLILIEFSINISKL
                     SEKNKVLAQEMALLQNELEKLKAEKQADDPVRGDDLEDVEPVDKPVES"
     gene            complement(1059..1775)
                     /locus_tag="PKM34_09600"
     CDS             complement(1059..1775)
                     /locus_tag="PKM34_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ83886.1"
                     /translation="MKKILVIVPAFNESENIREVISKLHSENAGWDILVINDASEDKT
                     SEIARATGQAEVIDLPFNLGIGGCVQTGFRYARRNGYDFALQFDGDGQHSADEIHKLL
                     ELVRSGEADVAIGSRFRQKHDGFKSSSSRRMGIRIFEWFSFILIRQRITDHTSGFRAY
                     NRKALKFLADYYPSDYPEPEVVILLGRNGFTIRETFTQMHERKGGVSSIPITKGPYYM
                     IKVMLSMFMSAIRSKVYRYE"
     gene            complement(1800..>3290)
                     /locus_tag="PKM34_09605"
     CDS             complement(1800..>3290)
                     /locus_tag="PKM34_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ83887.1"
                     /translation="PSNKKAWKPWAFYAGAFLAGFFGFLSKQNAITFPIAFLLVELFF
                     IRDKEMRIDKKFLIAASSIIGIIIVFGLIINGLPREFDRISRSDYLVTQFRVLVKYWQ
                     LLILPVNQHLDYYWPVSQSLWGVKELVSLGVLLLTVALGVWLFMKKWYIASFGIFWFY
                     LTLSLESSIIPIRDLIFEHRLYLATFGFGFVISYLAFYFLAPKNIKYPIIVLSILALA
                     YIGASLNRNSVWKNPYTLWTDSIKKSPKRERAHYWLATYYMEVKDNEKAMESYNTSIE
                     CNPGFPLAYNGRANLKKELGKNKEAIEDYNKAIKLDPNYITAYYNRGITYAAMNKFDK
                     AINDYNKSIQLGNNASSVYYNRANSKRKNKDMKGALEDYNTSIKIDPNFPLCYFNRGL
                     TRAQMGDHVEAIKDIDRAIKMDPRNHLFYNGKGVSQISLQQYKEAIGNFDKCIQLNPN
                     FGQAYYNRGYAKQKGFNDKDGACKDWEISVAKGYKAARSYIQQYCK"
BASE COUNT          783 a          822 c          845 g          840 t
ORIGIN      
        1 tcagagttgt aaaaatatcg aatatattgc tatcgacggc ggctcaaccg acggtacagt
       61 ggagttcctg cgctcgctcg gcgactgtgt ccgctgggtt tcagagcccg accgcgggca
      121 gtcggacgcc gtcaataaag ggatgcagat ggccacgggc gacatcatcg gctggctcaa
      181 ctccgacgat ctctacctgc cgggggcatt gcaaacggtc gttcgtcatt ttcacgccaa
      241 tcccgattgc caatgggcct atgggcgctg tatcatcatc gacaaagagg gcgtcgagcg
      301 gtggaagtat atcacccgtt ataaaaacat ccggttaagg aaattcacac tggataagct
      361 ttatcgcgag aacttcatct cccagccggc ggtctttttc cgcaaagatc tgtggaacca
      421 ggcgggaggg ctagatctct cactgcacta cgccatcgac tatgatctgt ggcttcggtt
      481 tgcccttcgc tcaccggcat tggtcatcga cagcgacctg gcggcgttcc ggcggcacgg
      541 cgcctcaaaa agcgaaacag gattcaggga ccagttctat gaacagtatg ctgttgcaaa
      601 acgttatcat ccctcaggat tcaaccggct tatccaccgg ttcaacatct tcaagatcat
      661 cacctcttac cggatcatcg gcctgttttt ccgcttttaa tttttccagt tcattctgca
      721 gcaacgccat ctcctgggcc agcactttgt ttttttccga aagttttgag atattgatgg
      781 aaaactcaat gaggatgagg aagatagcca gtaaaagaat aaggaacagt gccgcagggg
      841 gataagcgat acccatcagg ccggcgaaat attcgagccc ttcgcgccag atggaaaaaa
      901 caatgaagac gacgctggag aatagccaca ggagcgagta ttcttctttt atcttctttt
      961 ttcttacaag atacaggata aacaggaaca gcccgacact gaccaggatc gcgatgacct
     1021 gcgtggtctg gatatttttc ccgatagaac ccagcgactc attcataacg gtaaacttta
     1081 gaccggatgg ccgacataaa catagaaagc atcactttaa tcatgtaata tggccctttg
     1141 gtaatgggga tcgacgatac gccgcccttc cgttcgtgca tctgggtaaa ggtttccctg
     1201 atcgtgaatc cgttgcgccc caggaggatg accacttcgg gctcaggata atcgctcggg
     1261 taataatcgg ccaggaactt caatgctttc cggttgtagg ccctgaatcc cgaggtatga
     1321 tcggtgatcc gctgccggat aagaatgaat gaaaaccatt cgaatatccg gatgcccatc
     1381 cgtcttgatg aggaggattt gaacccgtcg tgcttctgcc ggaacctgga acctatggct
     1441 acatcggcct cgccgctgcg caccagctcc agcaacttgt ggatctcatc ggcactgtgc
     1501 tgcccgtcgc cgtcgaactg caaagcaaaa tcgtacccgt tcctgcgggc ataccggaag
     1561 ccggtctgca cacacccgcc gatccccagg ttgaacggca ggtcgatcac ctccgcctgg
     1621 ccggtcgcac gggctatttc gctggtctta tcttccgatg cgtcgttgat caccaggata
     1681 tcccagcctg cattctcgct gtgaagcttc gaaatgacct cccggatatt ctccgattca
     1741 ttaaatgcag gaacgatgac cagtatcttt ttcatcgcat tcttccggtt cggaaaattt
     1801 tatttgcaat attgctgaat gtatgatctg gcggctttat accccttcgc aaccgatatc
     1861 tcccagtcct tgcaggctcc gtctttatca ttaaatccct tttgtttggc gtacccgcgg
     1921 ttgtaatagg cctggccgaa attgggattg agctgaatgc acttgtcgaa attcccgata
     1981 gcttctttgt actgctggag cgaaatttgt gaaacccctt tgccgttgta gaacaggtgg
     2041 ttcctgggat ccatcttgat cgcgcggtca atatccttga tcgcctccac atggtcgccc
     2101 atctgcgccc gggtcaaacc ccggttgaaa tagcaaaggg ggaagttggg atcaattttg
     2161 atggaggtgt tatagtcctc cagcgcgcct ttcatatcct tgttttttct tttggaattt
     2221 gccctgttgt aataaaccga cgaagcattg ttgccgagct ggatactttt gttatagtca
     2281 ttaatagcct tgtcgaactt attcatcgcg gcataggtga ttccacggtt atagtaggct
     2341 gtaatatagt tcgggtcgag tttgatcgct ttgttgtaat cttctatggc ttctttattt
     2401 tttcccagtt ctttcttcag gttggccctg ccgttgtaag ccagcggaaa tcccgggttg
     2461 cattcgatgg aggtattata gctctccatc gctttttcat tgtcctttac ttccatataa
     2521 taggtggcaa gccagtagtg cgccctttcc cgcttgggcg atttcttgat gctgtcggtc
     2581 cagagggtgt atggattctt ccatacgctg ttgcggttaa gggaagcacc gatataagcc
     2641 agggcaagaa tggaaagtac aatgattggg tacttgatat ttttcggggc caggaaataa
     2701 aaagcgagat agctgatcac aaagccaaat ccgaacgtgg ccaggtagag cctgtgttcg
     2761 aagatgaggt cgcggatcgg gataatactc gattcgagcg acagggtcag gtagaaccag
     2821 aagatgccga aggaggcgat ataccatttt ttcatgaaca gccagacgcc gagagccaca
     2881 gtcagcagca atacgccgag gctcaccagc tctttaacgc cccagagcga ttgagagaca
     2941 ggccagtaat aatcgagatg ctggttgacg ggcaggatga gcagttgcca gtatttgacc
     3001 agcacccgga actgggtgac gagataatcc gaccggctga tccggtcgaa ctcccgtggc
     3061 aggccattga tgatcagtcc gaagacgatg atgatgccga tgatggaaga ggcggcgatc
     3121 aggaactttt tatcgatcct catttcttta tcgcggatga agaacaactc cacgaggagg
     3181 aaggcaatgg ggaaggtgat ggcattctgt ttcgacagga aaccgaagaa cccggccagg
     3241 aaggcaccgg cataaaaagc ccagggtttc caggcctttt tgtttgacgg
//
LOCUS       DAOQXS010000357         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_30721_length_3285_cov_2.617337, whole genome shotgun sequence.
ACCESSION   DAOQXS010000357 DAOQXS010000000
VERSION     DAOQXS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30721_length_3285_cov_2.617337"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..856)
                     /gene="map"
                     /locus_tag="PKM34_09610"
     CDS             complement(65..856)
                     /gene="map"
                     /locus_tag="PKM34_09610"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNQ83888.1"
                     /translation="MIYFKSKEEIELIRESSLLVGKTLAEVARVIAPGIRTIVLDKLA
                     EEFIRSHGAEPGFKGYNGFPFTLCISVNEQVVHGFPGDRVLQEGDIVSVDCGVLKNGF
                     YGDSAYTFAIGEVDSQVKDLMRKTKESLYLGIEAAVDGKRVGDIGYAVQNYVEEFGYS
                     VVRDLVGHGVGRNLHEKPEVPNYGRRGTGVRLKEGMVIAIEPMINLGGKNVVQERDGW
                     TIRTADRMPSAHFEHTIAIGTTRADVLSTFRYIEEELTKRGIEII"
     gene            complement(859..2217)
                     /gene="secY"
                     /locus_tag="PKM34_09615"
     CDS             complement(859..2217)
                     /gene="secY"
                     /locus_tag="PKM34_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ83889.1"
                     /translation="MKRLINTLRNIYKIEDLRKRILYTLGIIVIYRLGSYVVLPGVDP
                     NMLAQLQSQTSGGLLGLLSMFTGGAFSNASIFALGIMPYISASIVIQLLGMAIPYFQK
                     LQREGESGRRRINQITRYLTVVITAFQAPAYIRGVIIANLPPEAIAPFDPNATSPSTF
                     FWFSSIVILITGTMFVMWLGEKITDKGIGNGISLIIMIGIIARLPFALAGEFASRIAS
                     QGGGLVIFTIELVVLVFIILATILLVQGTRRIPVQYAKRVVGNKQYGGVRQYIPLKVN
                     AAGVMPIIFAQAIMFLPITIAGFTNTESMSAFAAAFANPYGLWYNVVYFLLIIMFTYF
                     YTAITVNPNQMAEDMKKNGGFIPGVKPGKKTAEFIDNVMSRITLPGSIFLGFVAIMPS
                     LVINMGVNSQFASFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLTKTGRIKGRSAY
                     TM"
     gene            complement(2226..2672)
                     /gene="rplO"
                     /locus_tag="PKM34_09620"
     CDS             complement(2226..2672)
                     /gene="rplO"
                     /locus_tag="PKM34_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ83890.1"
                     /translation="MDLSNLKPAKGSTKTSKRIGRGQGSGKGGTSTRGHKGAKSRSGY
                     SRKVGFEGGQMPLHRRVPKWGFKNRNRVEYRAINLDVLQKLAEDKNITAIDPEILFTN
                     GLVGKNDLVKILGRGELRAKIEVKAHAFSDKAKQAIEAVNGVASLI"
     gene            complement(2696..2875)
                     /gene="rpmD"
                     /locus_tag="PKM34_09625"
     CDS             complement(2696..2875)
                     /gene="rpmD"
                     /locus_tag="PKM34_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNQ83891.1"
                     /translation="MKKVKVTQIRSGIGQTLRQKRTLEALGLKRMHHTVEHEATPQIM
                     GMCKKVEHLVKVEEV"
     gene            complement(2894..>3285)
                     /locus_tag="PKM34_09630"
     CDS             complement(2894..>3285)
                     /locus_tag="PKM34_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ83892.1"
                     /translation="VGDGKGTVGHGLGKAREVSEAIGKAVKDAEKNLVKVPLHKGTIP
                     HEAFGKFDAGRVLIKPAAHGTGVIAGGAMRAVFESVGIKDVLAKSKGSSNPHSVVKAT
                     INALAKMKDARTVAQLRGVDLNTVFNG"
BASE COUNT          749 a          890 c          763 g          883 t
ORIGIN      
        1 tggcattccc caacgattcg atgaccgttc cgtcctgttc tatagaagcc tgtttagcca
       61 tatattaaat aatttcaatt cctctttttg taagttcttc ctcgatatac cggaaagtcg
      121 acagcacatc cgcccgggtc gtaccgatgg caatcgtatg ctcaaaatga gctgaaggca
      181 tccggtcagc ggtcctgatg gtccatccgt ctctttcttg aacgacattt tttccgccaa
      241 ggttgatcat cggttctatg gcaatcacca tcccttcttt aagcctgacc ccggtaccgc
      301 ggcgaccgta gttgggcacc tccggctttt cgtgcaggtt tcgccccacg ccatgtccga
      361 ccaggtctct caccacgctg tagccaaatt cctccacata attctgtacc gcatatccga
      421 tatctccgac ccttttcccg tcaacagccg cttcgatccc caggtatagc gattcttttg
      481 tttttcgcat caaatctttc acctggctgt ctacctctcc tattgcaaag gtataggcag
      541 agtcgccgta aaaaccgttt ttcagcaccc cgcaatcgac cgagacaata tctccttcct
      601 gcagcaccct gtcgcccgga aaaccgtgaa ctacctgctc gttgaccgag atgcaaaggg
      661 tgaaaggaaa tccgttgtat cctttaaatc cgggttcagc cccgtgactc cggataaatt
      721 cctcggccag tttatccagg acgattgtcc ggatacccgg tgcaataacc cgggcaacct
      781 cggctaatgt ctttccaaca agtaaagaac tctctcttat taattctatt tcttctttcg
      841 acttgaaata gatcatttct acatggtata tgccgaacgt ccctttattc ttccggtttt
      901 ggtcaaaccg tcgtagtgac gcatcagcag atgactttct atctgttgca gggtatccag
      961 cacaacgcct acgagaataa gcagtgatgt tcccccgaaa aatgatgcaa actgagagtt
     1021 aacgcccata ttgatcacca atgaaggcat tatggccaca aacccaagga aaatagatcc
     1081 cggcagtgtg atcctcgaca tcacattatc tataaattcg gccgttttct tcccgggttt
     1141 cacgccggga ataaatcccc cgttcttttt catatcctct gccatctggt tcgggtttac
     1201 ggtaatagcc gtatagaaat aggtgaacat aatgatcagc aggaagtaga ccacgttgta
     1261 ccagagcccg taaggattgg caaaagcggc ggcaaaagcc gacatgcttt cggtgttggt
     1321 aaatccggcg atggtgatcg gcaggaacat gatggcctgt gcaaaaatga tcggcataac
     1381 gccggccgca ttcactttca gcggtatata ctgcctgacg ccgccatact gtttgttgcc
     1441 gacgacccgt ttggcatact ggaccggaat tcgccgggtc ccctgcacaa gcaatattgt
     1501 tgccaggatg atgaacacca gcaccaccag ttcaatggta aagatgacca gaccgcctcc
     1561 ctggctggca atgcgtgaag caaattctcc ggcgagggca aagggcagac gggctatgat
     1621 cccgatcata atgatcaggg agataccgtt tccaatcccc ttatcggtga tcttttcacc
     1681 cagccacatg acaaacatgg taccggtgat caggataacg atagaagaga accagaagaa
     1741 agtcgaaggt gaagtggcat tcggatcgaa cggcgcaata gcttcggggg gaaggttggc
     1801 aatgataacc ccgcggatat atgccggtgc ctggaatgct gtaataacaa cggtcaggta
     1861 ccgtgtgatc tggttgatcc ttctccttcc gctttccccc tcacgctgca gcttctgaaa
     1921 atacggaatg gccataccca gcagctggat gacgatggat gcggaaatgt agggcatgat
     1981 accgagagcg aatatggaag cattcgaaaa tgcgccgccg gtgaacatac tcagaagccc
     2041 gagcaatccc ccggatgtct ggctctgtag ttgtgccagc atgttcggat ctacacccgg
     2101 aagcaccaca tatgaaccca gtcgatatat aacgatgatc ccgagagtat aaaggatacg
     2161 cttgcgaaga tcctcgatct tatatatgtt tcttaaggta tttattaatc ttttcattat
     2221 ttgcgttaaa ttaatgatgc aacaccattc accgcttcga tggcctgttt ggctttatcg
     2281 gaaaaggcat gggccttgac ttcgatcttt gctctgagtt cgccacgtcc caggatcttt
     2341 accaggtcgt tctttccaac gaggccattc gtaaagagga tttccgggtc aattgccgta
     2401 atatttttat cttcagccag tttctgcagc acatccaggt tgattgccct gtactccacc
     2461 cggttacggt ttttgaagcc ccatttgggc actctgcggt gaaggggcat ctggccgcct
     2521 tcgaaaccga ctttccggct gtaacccgac cttgacttgg cccctttgtg gccacgtgtg
     2581 gaggttccac ccttgccgga gccctgtccg cgtccgatac gtttgctggt ctttgtcgac
     2641 ccttttgccg gtttaagatt gcttaaatcc atcttctgtc aatatcgcta aatgattata
     2701 cttcttccac tttaacaagg tgttccacct ttttgcacat ccccatgatt tgcggggtgg
     2761 cttcatgttc gacggtatgg tgcatccgtt tcaggcctaa cgcttccaga gtgcgtttct
     2821 gccgcaacgt ttgtccgatc ccgcttctga tctgggtaac cttaactttt ttcattgtca
     2881 tttcctttcc agattatccg ttgaatactg tattgaggtc gacaccgcgc agctgggcca
     2941 cggtccgtgc atctttcatt tttgccagtg cattgatggt tgctttaacc acgctgtgcg
     3001 gattggaaga gcctttcgat ttggccagca cgtctttgat accaacgctt tcgaagacgg
     3061 cacgcatagc gccgccggca atcacccccg taccgtgagc tgcaggtttg atcagtacgc
     3121 ggccggcgtc aaatttacca aacgcctcgt gcgggatagt acctttgtgg agcggcactt
     3181 tcacgagatt cttttcggcg tctttgacgg ccttgccgat ggcttcggag acctcgcggg
     3241 ctttgccgag gccatggccc acggtgcctt tgccgtcgcc cacaa
//
LOCUS       DAOQXS010000358         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31075_length_3259_cov_3.090512, whole genome shotgun sequence.
ACCESSION   DAOQXS010000358 DAOQXS010000000
VERSION     DAOQXS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31075_length_3259_cov_3.090512"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PKM34_09635"
     CDS             <1..151
                     /locus_tag="PKM34_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ83893.1"
                     /translation="MDKGFIEKPPLDMIRKALEKYNGRRKNAARELGISERTLYRKIK
                     EYDIS"
     gene            138..647
                     /locus_tag="PKM34_09640"
     CDS             138..647
                     /locus_tag="PKM34_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNQ83894.1"
                     /translation="MIFRNITGIFLLTAVMLTAGCGVYSFTGASIPPEAKTISVSYFV
                     NNAQYVEPTLSQSLTDALRDRFQSQTSLTFIAEDGDLQLEGSITDYSTKPMAIQGNET
                     AALNRLSVTIKVRFTNSIDPAKDYEASFTRFEDYSSNQDLSAVKDQLIPLINEALVDD
                     IFNKAVVNW"
     gene            664..1386
                     /locus_tag="PKM34_09645"
     CDS             664..1386
                     /locus_tag="PKM34_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83895.1"
                     /translation="MNSQQFYRWMDDPTALDSESLAELQQVAAVFPYFSTGRILYLLN
                     LKKLDDYRFEQELKNAAVFISDRSRLRGWINFLDDNENKNGGQTEILPLEPEQDDSLR
                     EIENQIRDNIREIEQKQMRLKELMEEKKTLLNMESGVPEKDKEQGNLPRPLPKDDLLE
                     DFLLQQQSHPSNKKSFYNPEDYARKSIEENDGIFSETLARLVAAQGKKEKAIKIYQQL
                     MLKYPQKSSYFAAQIEKLKKES"
     gene            1399..1725
                     /gene="secG"
                     /locus_tag="PKM34_09650"
     CDS             1399..1725
                     /gene="secG"
                     /locus_tag="PKM34_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ83896.1"
                     /translation="MGLYILVSVLVLVACILLILVVLVQNSKGGGLASNFGSTGQIMG
                     VRKTADFLEKTTWSLAIALLALSLISILVIPRNATTGSTQDTELREKIENMQPIPSDQ
                     VPPEQE"
     gene            1865..2134
                     /locus_tag="PKM34_09655"
     CDS             1865..2134
                     /locus_tag="PKM34_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNQ83897.1"
                     /translation="MSIKPLADRVIVEPAIAEEKTAGGIIIPDTAKEKPQRGTIVAVG
                     PGKKDEPLTVKVGDEVLYAKYAGTEFSYEGKNFLIMRESDIVAVI"
     gene            2173..>3259
                     /gene="groL"
                     /locus_tag="PKM34_09660"
     CDS             2173..>3259
                     /gene="groL"
                     /locus_tag="PKM34_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNQ83898.1"
                     /translation="MAKEIIFDLAAREKLKKGVDELSNAVKVTLGPKGRNVIIQKSFG
                     SPTITKDGVTVAKEVELKDNAENMGAQMVKEVASKTSDLAGDGTTTATVLAQAIFTTG
                     LKNVTAGANPMEIKRGIDLAVEKVIKSLKEQTKEIGDSFEKIEQVATISANNDFAIGK
                     LIAEAMAKVKKEGVITIEEAKSMETYVDVVEGMQFDRGYISPYFVTDTEKMEAVYDNP
                     FILIHDKKISIMKDLLPILEKAAQTGRPLVIIAEDVESEALATLVVNKIRGALKVVAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGYKLEDANLGYLGNAEKVSVDKENTTIVG
                     GKGQKENIDARVKQIKMQIENTTSDYDR"
BASE COUNT          983 a          744 c          783 g          749 t
ORIGIN      
        1 gatggataaa ggattcatcg aaaagccacc cctcgatatg atccggaaag cactggaaaa
       61 atacaacgga cggcggaaaa atgccgcccg tgagctgggt atatcggaga ggaccctcta
      121 ccgaaagatc aaggaatatg atatttcgta acattaccgg cattttcctg ctgaccgcag
      181 tcatgctgac ggccggctgc ggtgtctatt cgtttaccgg cgccagcatt cccccggaag
      241 cgaaaaccat ctctgtcagc tattttgtga ataatgccca gtatgtcgag ccaacgttga
      301 gccagagcct gaccgatgcc cttcgtgacc ggttccagtc gcagaccagc ctcactttta
      361 ttgccgaaga cggtgatttg cagcttgaag gttccattac cgattattcc actaaaccta
      421 tggctatcca gggaaatgaa acggctgcac tgaacaggct ttctgtcacg atcaaagtta
      481 ggttcaccaa ttcgatcgat ccggcaaagg attatgaggc cagttttaca cggtttgaag
      541 attattccag caaccaggac ctctccgcgg tcaaagacca gttgattcct ctcatcaacg
      601 aagcgctggt ggatgatatt tttaataaag ccgtggtaaa ctggtaagct taaaattgtg
      661 cttatgaata gtcagcagtt ttaccgttgg atggatgacc ccaccgctct cgactcagag
      721 agcctggccg aattgcaaca ggtggctgcc gttttcccct atttctcaac aggccgtatc
      781 ctgtacctgc ttaaccttaa gaaactggat gattaccggt ttgaacagga gctgaaaaat
      841 gcagctgtct tcatttcgga ccggtcccgg ctaaggggct ggatcaattt tttggatgat
      901 aatgagaata agaatggcgg ccagacagag attctgccgc tggaacctga gcaggatgat
      961 tcgttgcggg aaattgaaaa tcagatccgg gataatatcc gtgaaattga gcagaaacaa
     1021 atgcgcctga aggagctgat ggaagaaaaa aagacgcttc tgaacatgga atccggcgtt
     1081 ccggagaaag ataaggagca gggtaacctg ccccgccctt taccaaaaga cgatctgctt
     1141 gaggatttcc tgttgcaaca acaatcgcat ccttcaaata aaaaatcttt ttacaatcct
     1201 gaggattacg ccaggaagag tattgaggaa aacgacggta tttttagcga aaccctggcc
     1261 cgtctggttg ccgcccaggg caaaaaagaa aaggcgatta aaatttatca gcaactaatg
     1321 ttgaaatatc cccaaaaaag tagttacttt gcagcccaaa ttgaaaaact caaaaaagaa
     1381 tcataaaaat ttagcgaaat gggtttatat attttagttt cggtgctggt cttagttgct
     1441 tgtattctcc tgatactcgt cgttttagtt cagaattcca aaggtggcgg cctggcttcc
     1501 aatttcggtt cgaccggtca gatcatgggt gtccgtaaaa cggccgattt tcttgaaaaa
     1561 acaacctgga gcctggctat tgcccttctg gccctcagcc tgatctccat cctggtcatt
     1621 ccccgcaatg caacaaccgg aagtacgcag gatacggaac ttcgtgaaaa gatcgagaac
     1681 atgcaaccca ttccgtcaga ccaggttccg cctgaacagg aatgattgtt tgaacatatt
     1741 ttttttatga aatgtcagaa tgtcataaga ttctgacatt tctttttttt atccggtttg
     1801 gcataaatta tgacaaacct gagcggaatt ttaataatct aaaaactcta tttatatgac
     1861 aacaatcagc attaaaccgt tagcagaccg ggtaatcgtt gaacccgcaa ttgcagagga
     1921 aaagacagcc ggagggatta tcattcccga cacagcaaag gaaaaaccac aacgcggaac
     1981 catcgtggcc gtgggccccg gtaagaaaga cgaacctctt actgtgaaag ttggcgatga
     2041 ggttctctat gcaaaatatg caggcactga atttagctat gagggaaaaa atttcctgat
     2101 catgcgcgaa tccgacatcg ttgctgttat ctaattgaaa tttattaatt taaacttaaa
     2161 tatctgaaca tcatggcaaa agaaatcatt ttcgacctgg ccgcaagaga aaaactgaag
     2221 aaaggtgtcg atgaactatc caatgcagta aaagtgaccc tcggcccgaa aggacgtaat
     2281 gtcatcatac agaaatcatt cggttccccg accattacca aggacggcgt taccgttgcc
     2341 aaagaagtgg aactgaaaga caatgccgag aacatgggcg cccagatggt gaaagaggtg
     2401 gcttccaaaa ccagcgacct tgccggtgat ggtactacca cggctactgt cctcgctcag
     2461 gcaatcttta cgaccggact gaaaaatgtc acggccggcg ctaacccgat ggaaataaaa
     2521 aggggtatcg acctggctgt tgaaaaggtc atcaaatccc tgaaagaaca gacaaaagag
     2581 atcggcgaca gcttcgagaa aatcgagcag gtagccacta tttctgccaa taacgacttt
     2641 gccatcggta aactgattgc cgaagctatg gccaaagtga agaaggaggg cgtgatcacg
     2701 atcgaggaag ccaaaagtat ggaaacctat gtcgatgtgg tggaaggaat gcagttcgac
     2761 cgcggttaca tctctcctta ttttgtgacc gataccgaga aaatggaagc ggtgtacgac
     2821 aatccgttca tcctcatcca cgacaagaag atctccatca tgaaagatct gcttccgatc
     2881 ctggagaaag cagcacagac cggccgtccg ctggtgatca tcgccgagga cgtagaaagc
     2941 gaagccctgg caaccctcgt agtcaataag atcaggggcg ccctgaaagt tgtggcggta
     3001 aaggccccgg gattcggcga cagaaggaag gaaatgctcg aggatatcgc catcctgacc
     3061 ggcggtaccg taatctccga agaaaaagga tacaaactgg aagacgccaa cctcggttac
     3121 ctgggtaacg ctgaaaaagt gtcggtcgat aaggaaaata cgacgatcgt aggaggcaaa
     3181 ggacagaaag agaacatcga cgcccgcgtc aaacagatca aaatgcagat tgagaacacc
     3241 acttccgatt atgaccgtg
//
LOCUS       DAOQXS010000359         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31237_length_3248_cov_3.180708, whole genome shotgun sequence.
ACCESSION   DAOQXS010000359 DAOQXS010000000
VERSION     DAOQXS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31237_length_3248_cov_3.180708"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKM34_09665"
     CDS             <1..689
                     /locus_tag="PKM34_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNQ83899.1"
                     /translation="DEYVIQGGHLDAYDVATGGVDDGSGATPSMEAARLIMAAGGKPK
                     RTILTILWAGEEFGLLGSQSWVDRNMDQLGKISNMFNRDGGPTVITGISVPAAIREDF
                     EQICAPINGINPDFPFRIEERQPSEMPKKAWGTDSGPFAVAGVPVLHFNTGDPKGYNF
                     NYYEIWHTERDTYDKSIKEYQDHVSIVTAIVVYGVANLDHLLNREGYWKAPEEEQGKG
                     KKEKGKGGKL"
     gene            769..1710
                     /locus_tag="PKM34_09670"
     CDS             769..1710
                     /locus_tag="PKM34_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNQ83900.1"
                     /translation="MKALNFPVIATLILSMASYAAAGQGISVNKTGAPPDSSAILDIQ
                     STSQGVLFPRMSTWKIRNIQDPETGLMVFNTDSLELYIFTGQKWESVRDKTDTITTWE
                     CGQEFLYGGQAYSTVLIGTQCWMSENLNIGVRVEGTVEQHDNDTIEKYCYNNDPANCD
                     VYGGLYQWAEVVQYLNGATNFTSWDPAPEGPVRGICPEDWHIPSYGEWMELVVYCDYW
                     GGRLKETGTEHWFPPNTDANNESGFTAFPGGYRHPSNGGYAEFRYMGTLWSTTGYEPY
                     ETYSVYFIRLYFNNGGITWAIGIEKSWGFSVRCVMDN"
     gene            1779..>3248
                     /locus_tag="PKM34_09675"
     CDS             1779..>3248
                     /locus_tag="PKM34_09675"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ83901.1"
                     /translation="MHKILIIIGLIGICANIYCQEEAYNWYFGEQAGLSFAQGDPVAL
                     TDGALFTGEGCSAISSSSGELQLYSDGRFVYDRNHSIMPNGSGLLGHSSSTQSCIIVP
                     RPANNSQYYVFTVDAKDNGLVNGLCYSLVDMTLNGGLGDVSPAEKNISLLPYTCEKVT
                     AVRHNDGASVWVITHQWGSNAFYSFLVTAEGVNMTPVISHAGQAVSGDMEAAKGYIKV
                     SPDGARIAMANNTAFTVGIFSFDDASGVVSHIVTDDSYVNPGGSDPGGPYGVEFSPDS
                     DLLYISEWKTNRKISQYDLSSNDPQTILNSRVTVATVNQNADPFGAIQLAPDKRLYIA
                     RMGSNYMSAINFPDTAGPGCNFNDNAIELTGGNSRYGLPAFVQSYFNDIAAFTATPLK
                     DYAPLTVQFTDLSSGSPTGWKWDFENDGTFDAFIQNPSHTYDVPGIYTVKLTALYGFA
                     SDVAMKTNYISAAGGPELCLVTVTSEDANKLVWEKQTEIP"
BASE COUNT          812 a          834 c          850 g          752 t
ORIGIN      
        1 ccgacgaata cgtcatccag ggcgggcatc tcgacgcgta cgacgtagcc accggcggcg
       61 tcgacgacgg atcgggcgcc accccgtcga tggaagcagc ccggctgatc atggcggcgg
      121 gcggcaaacc gaaacggacc atcctgacga tcctgtgggc cggcgaagag ttcggcctgc
      181 tcggctccca gagctgggtc gaccgcaata tggaccaact cggcaagatc tccaacatgt
      241 tcaaccgcga cggcgggccg acggtgatca ccgggatcag cgtgccggca gccattcggg
      301 aagactttga acaaatctgc gccccgatca acggcataaa ccccgatttt ccgttcagga
      361 tcgaagagcg gcagccttcc gaaatgccca aaaaagcctg gggcaccgac agcggcccgt
      421 ttgccgtcgc cggcgtccct gtgctccatt tcaacaccgg cgatccgaaa gggtataatt
      481 ttaactacta cgagatctgg cacaccgaac gcgacaccta cgacaaaagc atcaaagaat
      541 accaggacca cgtatcgatc gtgacggcta tcgtggttta cggcgtggca aacctggatc
      601 atctgctgaa ccgtgaaggg tactggaaag cgccggagga ggaacaggga aaagggaaaa
      661 aggaaaaagg aaaaggggga aagctctgaa aatttctaat ttctattttc taatttctga
      721 tttttactta tttatcttat ctttatgggt ctgatattaa actcagccat gaaagcatta
      781 aatttcccgg tgattgcaac gctgatttta tccatggcat catatgcagc tgccggccag
      841 ggcatttccg tcaacaaaac cggcgctccg cccgactcct ccgccatcct cgacatccaa
      901 tccacctcgc agggcgtcct ttttccacgg atgtcgacct ggaaaataag aaatatccag
      961 gatccggaaa cgggcctcat ggtcttcaac accgattcgc tcgaattgta catcttcacc
     1021 ggccagaaat gggaatcggt ccgggataaa accgatacga tcacaacctg ggagtgcggt
     1081 caggaatttc tttacggggg acaagcctac agcaccgtat tgatagggac tcaatgctgg
     1141 atgtcggaaa acctcaatat cggtgtcagg gtagaaggga cagtggaaca gcatgacaac
     1201 gataccattg aaaaatactg ttacaacaac gacccggcca attgcgatgt ctatggcggg
     1261 ctctaccagt gggccgaagt ggtgcagtat ctgaacgggg cgaccaattt cacctcgtgg
     1321 gatccggcgc ccgaaggacc tgtcagggga atctgcccgg aagactggca tatcccctct
     1381 tacggcgaat ggatggaatt ggtggtatat tgcgattatt ggggcggacg gctgaaagag
     1441 acgggaacgg agcattggtt tccgcccaat acggatgcaa acaacgagag cggttttacc
     1501 gctttcccgg gcggttaccg gcacccatcc aacggcggtt atgccgaatt ccggtatatg
     1561 ggcacgttat ggtcaaccac agggtatgaa ccctatgaaa cctactcggt atattttatc
     1621 cgcttatatt ttaataatgg cggaataacc tgggcgatcg gcatcgaaaa atcatggggg
     1681 ttctcggtga ggtgcgtgat ggataattaa gtcgttggcc gctgtcctgt gtccgccaaa
     1741 ggcggacgaa ggaactctgg tcgttagtca ttagaagcat gcataagata ctcattatta
     1801 taggattaat agggatttgc gccaatatct attgccagga agaagcgtat aactggtatt
     1861 tcggcgaaca ggcgggtttg agctttgccc agggtgatcc ggtggcattg accgacggtg
     1921 cactttttac aggtgaagga tgttcggcta tctcttcgtc ttccggggaa ctgcagctct
     1981 actccgacgg caggtttgtg tatgaccgca accattccat catgccgaac ggcagcggcc
     2041 tgctgggaca ttcttccagt acccagtcgt gcatcatcgt tccaagaccg gccaataatt
     2101 ctcaatacta cgtatttacc gttgatgcca aagacaacgg gctggttaac ggcctttgct
     2161 attcccttgt cgatatgacg ctgaatggcg gactcggcga tgtgtcgccg gcagaaaaaa
     2221 acatctccct ccttccgtat acctgtgaaa aagtaactgc cgtcaggcac aatgatggcg
     2281 cctctgtctg ggtcattacc caccaatggg ggtctaatgc attttattcg ttcctcgtca
     2341 ccgcagaagg tgtaaatatg acgccggtca tcagtcatgc cggtcaggcc gtttcaggcg
     2401 acatggaggc ggccaagggt tatatcaaag tttctcccga cggcgccaga attgctatgg
     2461 cgaacaatac ggcttttact gtcggtatct ttagcttcga tgatgcatcg ggagttgtga
     2521 gtcatatcgt cactgacgat agttatgtga atccgggagg atccgacccc ggtgggcctt
     2581 atggcgtaga attctcgccg gatagcgacc tgctctatat tagcgaatgg aaaacaaacc
     2641 ggaagatcag ccagtacgac ctctcgtcca atgatccgca gactatcctg aattcacgcg
     2701 ttaccgtcgc cactgtcaac cagaatgccg atcctttcgg agccatccag cttgctcccg
     2761 ataaacggct ttatatcgca cgaatgggaa gcaactatat gtcggccatc aattttcccg
     2821 atacggcagg ccccggctgc aatttcaacg ataatgccat tgaactgaca ggaggaaatt
     2881 caagatatgg gcttccggcc tttgttcaat cttactttaa cgatattgcc gctttcaccg
     2941 ctactcccct gaaggattat gcccctttga cggtccagtt cacagatctt tccagtggca
     3001 gcccgaccgg ctggaagtgg gattttgaga acgacggcac tttcgatgca tttattcaga
     3061 acccttcaca tacgtatgat gtgccgggta tttacactgt aaaactgaca gcgctttatg
     3121 gtttcgcttc cgatgtggct atgaagacaa attatatctc ggctgcagga ggtcctgaat
     3181 tatgcctggt gacagtcaca tccgaagatg ccaataaact ggtttgggaa aaacagacgg
     3241 aaattccc
//
LOCUS       DAOQXS010000360         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31454_length_3231_cov_3.576511, whole genome shotgun sequence.
ACCESSION   DAOQXS010000360 DAOQXS010000000
VERSION     DAOQXS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31454_length_3231_cov_3.576511"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1133)
                     /locus_tag="PKM34_09680"
                     /pseudo
     CDS             complement(21..1133)
                     /locus_tag="PKM34_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(1149..2204)
                     /locus_tag="PKM34_09685"
     CDS             complement(1149..2204)
                     /locus_tag="PKM34_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ83902.1"
                     /translation="MKNLNRSKSILAFIILIPIFFACNNKGNEPAKPAAAKPVEVRAV
                     IAEPKPVENKIFSTGSILANEEVEIRAEVPGRVTRINFQEGSAVKKGDLLVKINDNEL
                     LAELKKLELDEKLASDDVFRKEKLIELKAVSQEELDIARGQLGTIQAQIELVKARLEK
                     TEIFAPFAGRIGLRYISPGGYISSAMLIARLLQTDPVKIEFTVPEKYIGSVSQGMEVA
                     FTIAGSDSVFSGQVYAIEPRIDPSTRSFTVRARCQNPGGVLVPGAFAKVTAILERIPD
                     AIVLPSDALIPDIKGEKAYLCRNGKATAVYVTTGIRTETDVQITSGINPLDTVITSGL
                     LQIREQMSVTPRINGKN"
     gene            complement(2274..>3231)
                     /locus_tag="PKM34_09690"
     CDS             complement(2274..>3231)
                     /locus_tag="PKM34_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83903.1"
                     /translation="KFQRNGYQTVIVEDTTALEGYSILDVEMSGYYFIAGQVFADDNA
                     IGSGFAYWYKLVEEEVVNIGAGMVGELGWYEFSSMQAGNYIIKAEPAPGSDYYELYLP
                     TYYGDVLHWEEATIIELNLSTDDAHINLVAATVATEGPGTVSGKIESNTRTAEIPVFL
                     RTLDHAIVYMAVTGTDGAYSFNNIPYGTYEVFAEIPGKSITPKTIIIDELHTVIENID
                     MMIMDTEIIFLGIQESDIFIHEPFIYPNPAKETLNVFTGLKKKTRVEITIFDVAGRIV
                     TERKLEAGADETIRLDASFLPSGLYLLKLASENDLRVIEFLK"
BASE COUNT          717 a          824 c          836 g          854 t
ORIGIN      
        1 catctttatc ttcctccgct gaccagggag atcgggatgg cgatgaccgg tatgagggtc
       61 gtacgccagg tgcggagaaa gataaagatg accatgacca cgaggccaaa ggcgatcagc
      121 aatgtttcga atacctctgt gatggcatta cggatggtct tagtggtgtc gatggccagg
      181 cctacggaaa tatcttccgg caggtccagt tttatctgat ccagccgttt gtaacattca
      241 tcggcaatgg cgatctggtt ggccccgggc tgcggggtga tggcgactcc taccagcggt
      301 actgcattgt tgccccgcat gatagaacgt tcgttttcag gtaccaacac ggcttttccg
      361 acgtcgcgga acctaaccag ggtgccgtca gattcccgga tgatcatgtc gttgaactct
      421 tcaggagtcg tcaaacgccc taaggttcta attgtcagtt ccgtacgata gccctcgaca
      481 cggcctgagg ggagctcgac attttcgcgc gtcagtgcat tcctgacgtc gaccggggta
      541 agctggtaac tggccagttt tgccggatcc atcagcagtt tgatggcata tttcttttct
      601 ccccagatgc ggatttcgct gacccccgga accgtttgca gcctttcttt aaaaacattg
      661 ttggccaggt cggagagttc catcaggttt cgttcgttgc tctgaagcat caaaacgagg
      721 atggggctgg cgtcggcgtc cgattttgtc acaacgggcg gatcggtgtc gggcggcagg
      781 ctccggatcg cccgggagac gcggtcgcgg acgtcgttgg cggcggcctc catgtcaacg
      841 cccagttcaa actcaaccgt gatattgctt cgtccgtcgg agctggttga tgtcagtgaa
      901 cggatgccgg cgataccgtt gatggattcc tccaacggtt cggtgatctg cgattcgatc
      961 acatcggcat tggcgccgat ataactggtg gataccgtga ccaccggcgg atcgacgctg
     1021 gggaactcgc gaacgccgag aaaagtgtat ccgatggcgc cgaagagcac cagcaggatg
     1081 gagatcacgg tagccaggac cggacggtta atgctaatag atgaaatgtt cataaccctg
     1141 gccccgcttt agtttttacc gttgatacgg ggggtgacgc tcatctgttc gcgaatctgc
     1201 agcagtccgg atgtgatgac agtatccagc gggttgattc cggaggtgat ctggacatca
     1261 gtttcggtgc ggatgccggt ggtgacataa acggctgtcg cttttccgtt ccggcagaga
     1321 taggcttttt cccccttgat atccggaatc agggcgtcgg aggggagcac gatggcatcc
     1381 ggtatccgtt cgagaatggc tgtgaccttg gcgaaagccc ccggcacaag cactccgccg
     1441 ggattctggc accgggccct gaccgtgaaa gagcgggtgg atggatcaat ccttggttcg
     1501 atcgcatata cctggccgga aaacacactg tcgcttccgg caatggtaaa ggcaacctcc
     1561 atcccctggc tcacactgcc gatatatttt tccggcacgg tgaattcgat cttgaccggg
     1621 tctgtttgca acaggcgcgc gatcagcatg gctgacgata tgtacccgcc tgggctgata
     1681 tatctgagac cgatcctgcc tgcaaagggg gcaaagattt cggttttttc aagccgcgct
     1741 ttcaccagtt cgatctgggc ctgtattgtg cccagctggc cgcgggcaat gtcaagctcc
     1801 tcctggctta ccgccttcag ttcgatcagt ttttctttcc ggaaaacgtc atccgaagcc
     1861 agcttttcat ccagttcgag tttcttcagt tctgccagta actcattgtc attgatttta
     1921 accagcaaat cccctttttt aaccgccgat ccttcctgaa aattgatccg tgtaaccctt
     1981 ccggggactt cagccctgat ctcgacttct tcgttggcca ggatgctgcc cgtagaaaag
     2041 attttattct ctaccggttt cggttcagca ataacggctc tgacctccac cggctttgct
     2101 gcagcaggtt ttgccggttc attgccttta ttattacaag caaagaagat cggaatgagt
     2161 ataataaagg ctaggattga tttactcctg ttgagattct tcatatggtc gaattgagcg
     2221 aaaacaaccc gcaaaagtag ttattttact ttggaaggag aacccaaaaa ccgttacttt
     2281 aaaaactcta taacccggag atcgttttct gaggcgagtt tcagcaggta aagccctgaa
     2341 ggaagaaatg acgcgtcaag ccggatggtt tcatcggctc ctgcctcaag ttttctttct
     2401 gtaactattc ttcctgctac atcaaatatt gttatctcca cacgggtttt ctttttcagc
     2461 ccggtgaata cattcagtgt ttctttggca ggattgggat aaatgaaagg ctcatggatg
     2521 aaaatatccg attcctggat tcccaggaag atgatctccg tatccatgat catcatatca
     2581 atattttcaa tcacagtatg tagttcatcg atgattattg ttttgggcgt gatggatttc
     2641 cccggaatct cggcaaaaac ttcgtaggtt ccgtaaggga tgttgttaaa ggaataggcg
     2701 ccatcggttc cggtaaccgc catatatact atagcatggt caagggtccg gaggaatacg
     2761 ggaatttcgg cagtccgggt attgctttcg atcttgccgg aaaccgttcc cggtccttct
     2821 gtagcaacgg tggcggcgac aaggttgata tgtgcatcgt cggtactcag gttcaactca
     2881 atgatcgtcg cttcttccca atgcagcaca tctccgtaat aagtaggcag gtaaagctca
     2941 tagtaatccg atccgggagc cggttcagcc ttgataatgt agtttccggc ctgcatgctg
     3001 ctgaattcat accatcccag ctccccgacc ataccggccc cgatattcac aacctcttct
     3061 tccacaagtt tataccagta cgcaaatcct gatccgatcg cgttatcatc cgcaaacacc
     3121 tgtcctgcaa tgaaataata tccgctcatt tcgacatcga gtatggaata gccctctaat
     3181 gccgttgtgt cttcaacaat cacagtctgg tatccgtttc gctggaattt g
//
LOCUS       DAOQXS010000361         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31467_length_3230_cov_4.010079, whole genome shotgun sequence.
ACCESSION   DAOQXS010000361 DAOQXS010000000
VERSION     DAOQXS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31467_length_3230_cov_4.010079"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            619..1377
                     /locus_tag="PKM34_09695"
     CDS             619..1377
                     /locus_tag="PKM34_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83904.1"
                     /translation="MVSAQVPMVQDCLGAIPVCQDIYIEENSYSGDGNYHYEIYEDLP
                     CNSACPGSCLAGEINSVWYIWNVRESGMLRFTIDPVQAGDDYDWAVYDLTDLGCADIY
                     TEYPLMQKSCNASGGYSNGITGISSEEGGDSDCNNCGITNRWNADLPVYEGRTYVLVI
                     ENLSSSSMGYWLEFSDSTAIISDDSPPVLDSVFISGIRCGVSEVTFSFSERVACEAVN
                     PDDFLLTGPGGTYDILDVQGSVCMQGGEMDEEFT"
     gene            1361..1903
                     /locus_tag="PKM34_09700"
     CDS             1361..1903
                     /locus_tag="PKM34_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83905.1"
                     /translation="MRNLLDLGAPVVVDTGIVITPAGYGLNNGHITGLTVIGTEPLSY
                     RWFDHINDTVGTALDLHDVFAGNYFLQVTDTNYCVTDAGPYLVDLVEHVSEANSGPSG
                     IISVFPNPNGGTFTIHIGSDVARISVINMLGEIVYTFDNGRPYDQVISVDLRQNGNGI
                     YRIRVVSLSGQSVSKVVQVF"
     gene            complement(1911..3044)
                     /locus_tag="PKM34_09705"
     CDS             complement(1911..3044)
                     /locus_tag="PKM34_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ83906.1"
                     /translation="MDRIIYPRTLLDDIKTWMFKGKVLIIYGPRQVGKTTLAKILMSE
                     NQGALYLNCERPEVWEVLRSRNLERIMSYLGNTRMVVFDEAQKVPGIGELLKLLIDTY
                     PDTQYIASGSSSFNLISDLSEPLTGRNIKFMLYPLSLRELSHTFDPFSMDEKLPELLR
                     FGSYPDIVNRSESEKIRLLGELTSDYLFRDVLNFENIRKSDVLVSLLKAIALQIGQEV
                     SFREISGLLRLSVDTVQKYIQLLEKSFVIFSLSSFSRNLRKEIGTSRKYFFYDLGIRN
                     SLINNFNPPELRADTGQLWENYCILERIRHNQAVGRNVNYYFWRTYDQKEIDLIEETG
                     GMFTAFEIKWKSKKLKPVKDFLNAYPGSEFHLICKENYREFLV"
BASE COUNT          860 a          706 c          723 g          941 t
ORIGIN      
        1 tttaaatcaa ctatgaatga cagatccctg gtctagaaaa tggggagtac tttaccgaag
       61 ccggatactg cgccgatgat ggattgcggg atcgtattta cggacaaaaa gcatgaatac
      121 tttattcctc ttaaaaataa cagaccttag atgttaattc gcccacaggt tggggatgca
      181 tgacgaagaa atttaaaatt gccggcaatt tttataattt gtaaccgagt ggctaatcag
      241 ggtttggatt aatttttcca aatttataaa tttatattct gctgaaatga attttaaaaa
      301 tctaatctct ccctcccgcg gtaatttatt tgtaataagg cttatttccg attcactatg
      361 cacgatttcg aaaggatccc tgcaaccaaa atttcatcaa gcctcaacaa tcttacctca
      421 ttactcatta acccattact aattataaca ttacgcatta ctaattaatc attaaacatt
      481 aagcattaat ataacctcca attgcttata tttgaactca ctaattaatc cgcaacagcg
      541 atgaaaccgg ttttaatcaa ggtcaggttt tgtttattcc tcatttttca tttttcattt
      601 ttcatttttc atttttcatt ggtaagcgca caagtgccta tggtgcagga ttgcctgggt
      661 gccatcccgg tctgccagga tatttatatt gaagagaatt catacagcgg cgatggcaat
      721 tatcactatg aaatttatga agaccttccg tgcaacagtg cctgtccggg cagttgcctg
      781 gccggagaaa tcaacagcgt ctggtatatc tggaatgtca gggaaagcgg aatgctccgg
      841 tttaccatcg atccggtgca ggccggcgat gactacgact gggcagtgta tgacctgacc
      901 gacctgggct gcgcggatat ttacaccgaa tatccgctga tgcaaaaaag ctgcaatgcc
      961 tccggcggtt attcaaacgg aattaccggc atcagcagcg aggaaggcgg tgactcggat
     1021 tgcaataatt gcggcatcac aaaccgttgg aatgccgatc ttccggttta tgaagggagg
     1081 acttatgtgc tggtcatcga aaacttgagc agttcctcaa tgggttattg gctggaattc
     1141 agcgactcca cggcaattat cagcgatgat tcgccgcctg tgctcgattc tgttttcatc
     1201 tccgggatca ggtgcggcgt ttcggaagtt actttttcgt tttcggagag ggttgcttgt
     1261 gaggcggtta acccggatga ttttctcctc accgggcccg gcggcacgta tgacatcctg
     1321 gatgtgcagg gaagcgtgtg catgcaaggg ggcgaaatgg atgaggaatt tacttgacct
     1381 gggcgctccg gtggtcgtgg ataccgggat tgtcattact ccggccggtt atgggttgaa
     1441 caacggccac attaccggat tgaccgttat cggaacagaa ccgctgtcat atcgctggtt
     1501 tgatcacatc aacgatactg ttggaaccgc actggatttg catgatgtat ttgcgggcaa
     1561 ttattttctg caggtcaccg atacgaatta ctgcgtaacg gatgcgggac catacctggt
     1621 ggatctcgtg gagcatgttt ctgaagcgaa ttccggccca tcggggatta tttcggtctt
     1681 tcccaaccca aatggcggaa cttttactat tcacattggg agtgatgttg caaggatctc
     1741 cgttataaac atgctgggag agattgttta tacttttgat aatggccggc catacgacca
     1801 agtcatttca gtggatctgc ggcaaaacgg caacgggatc tatcggatcc gggttgtttc
     1861 cctcagcggt caatctgttt caaaggtggt acaggtgttt tgaggtgttt tcaaactaaa
     1921 aactcccggt aattctcttt gcagatcagg tgaaattcag agccgggata ggcatttaaa
     1981 aaatccttca ccggtttcag ttttttcgat ttccatttga tctcaaaggc ggtgaacatt
     2041 ccacctgttt cctcgatcaa atctatttct ttctgatcat acgtgcgcca gaaataataa
     2101 tttacatttc tgcccacggc ctggttgtgc ctgatccttt ccaaaatgca gtagttttcc
     2161 caaagctgtc ccgtatcggc ccgcagctcc ggtggattga aattattaat aaggctgttc
     2221 cggataccca gatcgtagaa aaaatacttt cgactggtgc cgatctcttt tctcaggttc
     2281 cggctgaaag aagagaggga aaaaataaca aatgattttt cgaggagttg gatgtacttc
     2341 tgtacggtgt caaccgataa tcttaacaat cctgatattt cccgaaacga tacctcctgc
     2401 ccgatttgca gcgcaatggc ttttaacagg ctgacgagaa catccgattt tcggatgttt
     2461 tcgaagttca aaacatcgcg gaacaggtaa tcgctggtca attcccctaa aagcctgatc
     2521 ttttccgatt cactgcggtt gacaatatcc ggataggaac cgaaccgtaa taattcaggc
     2581 aacttttcgt ccatcgaaaa tggatcgaag gtatgcgata gttccctgag tgaaagaggg
     2641 tagagcatga acttgatatt acggccggtg agaggctccg acagatcact gatcaggtta
     2701 aaactgctcg aaccacttgc aatatattga gtatcaggat atgtatcgat caatagctta
     2761 agcagttccc caatgcccgg taccttttgc gcttcgtcaa acacgaccat ccgggtgttg
     2821 ccaagatacg acatgatccg ttcgagattt ctggaacgca acacttccca gacctcagga
     2881 cgttcacagt tcaggtacaa cgccccctgg ttctcactca tgagaatttt tgcgagtgta
     2941 gtctttccaa cctgtcgcgg gccgtagata atcaaaactt tccctttgaa catccaggtt
     3001 ttaatgtcat ccaacagcgt cctggggtat atgatccgat ccataatgtt aaaattgtcg
     3061 ggtgtaattg acaaatatag caaaatatga ccaataggtc aaatgtttta ttaatcttat
     3121 gaatgaaagc atccggcgtt agtcgttagt cgttggttat tagtcattag tcattactca
     3181 ttaacccatt acgcattaat tcattactca ttaaacatta aacattaacc
//
LOCUS       DAOQXS010000362         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31507_length_3227_cov_3.329445, whole genome shotgun sequence.
ACCESSION   DAOQXS010000362 DAOQXS010000000
VERSION     DAOQXS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31507_length_3227_cov_3.329445"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PKM34_09710"
     CDS             complement(<1..948)
                     /locus_tag="PKM34_09710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ83907.1"
                     /translation="MHKKIDLLFALLLVSLAAFSQDKTPSFITDSLDNYIERSLRDWN
                     IPGAAVAVVHNDRVVVTKGYGFLEMGKKDKVDENSLFMIASNTKAFTGTAIAILESEG
                     KCSMDDRVQKYLPGFRMKDPWVAEHLTLTDVLSHRIGMETFQGDFMYWESELTSDQVV
                     EKFGQLTPVYDYRAKWGYCNAGFLIAGMCLEAMTGASWDAFIRTRFLQPLEMNRTLAL
                     TSEVKNATNLASAHTLVDGKLQVIPHCRIDNLAPAASISSSVSDMSHWLIALLDSGRY
                     GGRQVIPFKAIKSAQTPLSFVRKAGHPFNTAHYSLYGLGW"
     gene            1104..1358
                     /gene="rpsT"
                     /locus_tag="PKM34_09715"
     CDS             1104..1358
                     /gene="rpsT"
                     /locus_tag="PKM34_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNQ83908.1"
                     /translation="MANHKSALKRIRANENRKIQNRYHGKTMRNAIKKFRSIEAKEEA
                     TAQLPKLVSMIDKMAKRAVIHKNKAANLKSKLTRKVNAIA"
     gene            1519..2214
                     /gene="radC"
                     /locus_tag="PKM34_09720"
     CDS             1519..2214
                     /gene="radC"
                     /locus_tag="PKM34_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNQ83909.1"
                     /translation="MFYESRIPIKQWSEDDRPREKLLLKGKSALSNAELIALLLGSGN
                     RNESAVDLAKRVLDKSGQNLAELSRFQISDLIKIKGIGQAKGLTIIAALELGNRKRSQ
                     DALSREKVAGSHDVYELFHGELADAQYESFWVLLLNRANRVIRKVNISEGGISGTVAD
                     PKKIFKMSLEHNASSLILCHNHPSGNTQPSEADIRLTRKLKEAGLLLDLPVLDHLIVG
                     ADSYFSFADEGIL"
     gene            complement(2232..2639)
                     /locus_tag="PKM34_09725"
     CDS             complement(2232..2639)
                     /locus_tag="PKM34_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ83910.1"
                     /translation="MGFGFKDISQDILNNVLNDEDRCLAFIAEEKWRDGFVCRKCGHT
                     NYCKGKKPHSRRCTRCKQEESATSHTIFHGCHLPVTQAFRLAYQVCHNPSISTYELSR
                     QLETRQMTCWKLKKRMLECIETQGTLTVIPPNL"
     gene            2747..>3227
                     /gene="trxA"
                     /locus_tag="PKM34_09730"
     CDS             2747..>3227
                     /gene="trxA"
                     /locus_tag="PKM34_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ83911.1"
                     /translation="MKKPGYLIIFSTLLFISCSGTSNEKPANAAVNNTVQAKDGGDEQ
                     KETAAGKTIHLNAQTFKAKVMNYEKNQQQWVFEGDKPAIVDFYADWCRPCRMIAPIME
                     ELAAEYEGKIDIYKVDTDDERELAAVFGIQSLPSVLFIPMTGKPSLQKGAMSKEAYKQ
                     "
BASE COUNT          873 a          772 c          814 g          768 t
ORIGIN      
        1 ccatcccagg ccatacaaag aatagtgggc ggtattgaac gggtggccag cttttctcac
       61 gaatgatagc ggggtttgcg ccgatttaat ggctttgaaa gggatgacct gccggcctcc
      121 gtagcggccg ctgtcgagca gggcgatgag ccagtggctc atatcgctga ccgacgaact
      181 gatgctggca gccggagcca ggttatcaat ccggcagtgc ggaatgacct gaagtttgcc
      241 atcaaccagt gtatgggcgg aagccaggtt cgtggcattt ttcacttcgc ttgtcagggc
      301 cagtgtacgg ttcatctcca ggggctgcag gaagcgggtt cggataaaag catcccagct
      361 tgctccggtc atagcttcaa ggcacattcc ggcaatgagg aagccggcgt tgcagtagcc
      421 ccattttgcc cggtaatcgt agacgggtgt aagctgtccg aatttttcaa cgacctggtc
      481 gctggtcagt tccgattccc agtacatgaa atcaccctgg aaggtctcca ttccgatccg
      541 gtgcgacagt acatcggtga gtgtcagatg ctccgccacc caggggtctt tcatccggaa
      601 accgggaagg tatttctgca cccggtcatc catcgagcac ttaccctccg attcgaggat
      661 tgcgatcgcc gttccggtaa aggccttggt attggaagca atcatgaaaa gtgagttttc
      721 gtcgacctta tccttttttc ccatttcaag aaaaccataa ccttttgtga ccaccaccct
      781 gtcgttatgc accacggcca cggccgcgcc cgggatattc caatccctga gtgatcgttc
      841 gatgtaatta tccaggctgt cagttataaa agaaggtgtt ttatcctgag aaaaagcagc
      901 caggctcacc aggagtaatg caaacagcaa atcaatcttt ttatgcatac ttttttttcg
      961 gtaaaaataa tcataacccg ttaatttttc ctttggcttc cggtatcagc acccagtctt
     1021 ttcaaatatt ttcataaaac tttttctatt gtaaaaataa tctatacctt tgcaccccca
     1081 attggaatat aaaacgctaa aacatggcaa atcataaatc ggcgctgaag aggatcaggg
     1141 ccaatgaaaa caggaaaatc cagaatcgtt accatggcaa gaccatgcgt aatgccatta
     1201 agaaattcag atccattgaa gcaaaggaag aggcgactgc ccaactgccc aagctggtat
     1261 cgatgatcga caagatggcc aagcgcgctg tcatccacaa gaacaaagcg gcgaacctga
     1321 agtcgaaact gacccgcaaa gtcaacgcta tcgcttaaag aattttcaga ctgttttacg
     1381 gctgcctgtc gcccgacagg cagctttttt tatttccatt tgttattata aatcaatttg
     1441 ttatccgggt cataaaactt ttttttacca cgaccgggta ttttcagccc tttttccgga
     1501 taaacaggaa aaaagggtat gttttacgaa tcaagaattc caatcaagca atggtcggaa
     1561 gatgaccggc ccagggagaa gctattgctg aaaggcaaat cggctctgag caatgccgaa
     1621 ctgatcgctt tgctgctggg ttcaggcaac cggaatgagt cggccgtgga cctggctaaa
     1681 agggtcctgg acaaaagcgg gcaaaacctg gcggagcttt cgcggttcca gatcagcgat
     1741 ttgatcaaga tcaaaggaat cgggcaggcc aaagggctga cgatcatagc ggcgctggaa
     1801 ctgggcaacc gtaaacgaag ccaggacgcg ctatcaaggg agaaagtggc cggtagccac
     1861 gatgtgtatg agctgttcca tggcgagctg gccgacgcac agtatgaatc attctgggtg
     1921 ctgctgctga accgggcaaa ccgggtcatc cggaaagtca acatcagcga aggagggatc
     1981 tcgggtaccg tggccgatcc gaagaagatc ttcaagatgt cgctggaaca caatgccagc
     2041 tcactgatcc tctgccataa tcacccgagc ggcaacaccc agccgagtga agcggatatc
     2101 cggctgaccc ggaaactgaa ggaggccggg ctgctgctcg acctgccggt gctggatcac
     2161 ctgatcgtcg gtgccgacag ctattttagt tttgcggatg agggaatact ctagtgagtt
     2221 tgggttgcga gttataagtt tggagggatg acagtgaggg tgccctgggt ttcaatgcat
     2281 tccagcattc tctttttcag cttccagcat gtcatctggc gggtttcaag ctgccgggaa
     2341 agctcatagg ttgagatgga aggattatga caaacctggt aagccaggcg aaaagcctgt
     2401 gtaaccggca gatggcagcc atgaaaaatg gtgtgcgatg tggccgattc ttcctgcttg
     2461 caccgggtgc atctcctgga atggggcttc ttgcctttgc agtaatttgt atgcccgcat
     2521 ttccggcaca caaacccatc gcgccatttt tcttccgcaa tgaatgccag gcagcggtcc
     2581 tcatcgttca atacattatt taatatatcc tgtgatatgt ctttaaaacc aaatcccatg
     2641 cgggtaaaaa aatttttgac aaaaataaaa aaaatagtga tactaatgcg ctttaatatc
     2701 aaaatatatt ttacctttgc cgcatgattt aaaaaataaa aagaaaatga aaaaaccagg
     2761 atatctcatt attttttcga ccttgctgtt catcagctgc agcggaacat ccaacgaaaa
     2821 accggccaat gccgctgtaa acaacaccgt ccaggcaaaa gacggaggtg acgaacaaaa
     2881 ggaaactgca gccggaaaga ccattcatct caatgcccag acttttaaag ccaaggtgat
     2941 gaattacgaa aagaaccagc agcaatgggt ttttgaaggc gataagccgg caatcgtcga
     3001 cttttacgcc gactggtgcc gcccctgccg gatgattgcg ccgatcatgg aggaactggc
     3061 cgccgagtat gaagggaaaa ttgatattta taaggtggat accgacgatg agcgcgaact
     3121 ggcggccgta ttcggcatcc agagccttcc ctcggtgctc tttataccga tgaccggcaa
     3181 gccgtcgttg caaaaaggag ctatgtcgaa agaggcttat aaacaga
//
LOCUS       DAOQXS010000363         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31713_length_3213_cov_3.731476, whole genome shotgun sequence.
ACCESSION   DAOQXS010000363 DAOQXS010000000
VERSION     DAOQXS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31713_length_3213_cov_3.731476"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PKM34_09735"
     CDS             <1..1012
                     /locus_tag="PKM34_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNQ83912.1"
                     /translation="ICAIMGIEDFDIGDTIADAEAPEALIPIKVDEPTMSMLFTINNS
                     PFFGKDGKFVTSRHLRDRLFREIEKNLALRVEETGSPDSYMVFGRGILHLSILVETMR
                     REGFEFQLGQPQVILKEVDGVKHEPIEYLTVHVPEAFSGKVIDVVTGRKGEILHIETK
                     GDRIVLEFNIPARSIIGLRNTILTSTEGEAIIAHRFKGYEPWKGDLAVKRNGALIAME
                     TGLAIAYAIDKLQDRGSFFVEPNDPVYTGQVIGEHIHPNDLVINVCKTKKLSNMRASS
                     ADDKTNISPAIRFSLEEAMEYIRNDEYVEVTPKSIRLRKIILDENERKRKKIWAMQDA
                     QA"
     gene            complement(1045..2697)
                     /locus_tag="PKM34_09740"
     CDS             complement(1045..2697)
                     /locus_tag="PKM34_09740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ83913.1"
                     /translation="MRDEKSNQASKNLTENNLFVYICLAIAVIVVLIARLHLLSFPLE
                     RDEGEYAYFGKLILDGHAPYTLAYNMKLPGTYYMYALLMGIFGESIVGIHLGLTLIVT
                     ASMFLVFLIAKSFLSKIGAVMATAVFGIIGTSYTLLAQAAHATHFVVFFALIGFYILI
                     HHYKNNKRGWSVYFLAGLFFSFAFICKQSGLFFALFGATVVFVMEYGNISKAGMVKHL
                     ALFAAGFAALLLLMILYFALFADFDRFWFWTVKYLSKYNTQVPFSEAWVMLKKGVRNL
                     TADYTSAGYIVLWITSLIGLVTVFIRKTTMKTRILLISFFVFSFLTVVPGLYFRRHYF
                     ITLLPAAGFLVAFLFDYFNDIIINKLKKPALTIIPLLIFLIVAGSGVLANKEYLFTRD
                     PRVSCKQVYGSNPFVESVEIAEFIKRNTAPDDKIAILGSEPQIPFYADRYSATGYIYT
                     YNLVELHSYALNMQKEMIREIELNKPKLIIFVNVATSWLARPESEKYIFKWANEYLAR
                     NYKRVGLMDVYPNRASSLKVGDQLNNYKPQSGELIYVYERNP"
     gene            2842..>3213
                     /locus_tag="PKM34_09745"
     CDS             2842..>3213
                     /locus_tag="PKM34_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83914.1"
                     /translation="MTASKNISKREFIKASFFGGCGFCMAASGLNALAGSSFGNDSLS
                     HLAENGALPQDTPWKWSTPALYFVDTPKGIKCKLCPQKCEIEPGKAGDCRTNVNYNGV
                     LYSISYGNPCAVHIDPVEKKPL"
BASE COUNT          936 a          790 c          735 g          752 t
ORIGIN      
        1 aatctgcgcc atcatgggca tcgaagattt cgatatcggc gacacaattg ccgacgccga
       61 agctccggaa gcccttatcc ccatcaaggt cgatgaaccg acgatgagca tgctgttcac
      121 catcaacaac tcgccttttt tcggcaaaga cggcaagttc gtcacctccc gccacctgcg
      181 cgaccgcctg ttccgcgaaa tcgagaaaaa cctggctctc cgggtcgaag aaaccggctc
      241 acccgattcc tacatggttt tcggccgggg catcctgcac ctctccatcc tggtggaaac
      301 catgcgccgc gagggtttcg agttccagct cggccagccc caggtgatcc tgaaagaagt
      361 cgacggggta aaacatgaac cgatcgagta ccttacggtg catgtcccgg aagcattctc
      421 cgggaaagtc atcgatgtcg tgaccggaag aaaaggcgag atattacaca tcgaaaccaa
      481 aggcgaccgt atcgtgctgg aattcaatat cccggcaagg tcgatcatcg ggctgcgcaa
      541 taccatcctt accagtaccg aaggagaagc catcatcgcc caccgtttta agggttacga
      601 gccctggaaa ggcgacctgg ccgttaagcg caacggagcg ctgatcgcta tggaaaccgg
      661 actggctatt gcttatgcca tcgataagct gcaggaccgt ggttcgttct tcgtcgaacc
      721 caacgacccc gtctataccg gtcaggtcat cggggaacat atccatccca acgacctggt
      781 catcaacgta tgcaagacca agaaactcag taatatgaga gcttccagcg ccgacgacaa
      841 gacaaacatc tcccccgcta tccgtttttc actggaagaa gcgatggaat atatccggaa
      901 tgatgaatat gtggaagtta cgcccaaatc gatccgcctg agaaaaatca tcctggatga
      961 aaatgaaagg aaacgtaaga agatctgggc tatgcaggac gcccaggctt aaacccgttt
     1021 acaacagtat tacttcacct ggcctcaggg attcctttca tatacataaa tcagttcgcc
     1081 cgactggggt ttgtaattat tgagctgatc tccgactttt aatgaagatg cgcggtttgg
     1141 atatacatcc ataagcccca ctcgtttgta gttcctggcc aggtattcat tggcccattt
     1201 aaatatatat ttctcagatt ccggccgggc cagccacgat gtggccacat tgacaaagat
     1261 gatcagtttt ggtttattca attcaatctc ccggatcatc tccttttgca tgttcagcgc
     1321 atatgaatgc agctcgacaa gattgtaggt gtaaatatac cccgttgccg aataccggtc
     1381 tgcgtaaaaa gggatttgcg gctctgagcc cagtatggcg attttgtcgt ccggggcagt
     1441 atttcgcttg atgaattcgg ctatttcaac agattccaca aaaggatttg aaccgtaaac
     1501 ctgcttacat gaaaccctgg gatcgcgggt aaacaaatat tctttattgg ccagcacacc
     1561 gctgcctgct acgatcagga atatcaataa aggtatgatg gtcaatgcag gtttctttaa
     1621 tttattgata atgatatcat taaaataatc aaacaaaaaa gccaccagaa aaccggctgc
     1681 cggtaaaaga gtgataaaat aatgccttct gaaatacaac ccgggcacca cagtcaggaa
     1741 tgaaaacaca aaaaaggaga tcaggaggat ccgggtcttc atggttgtct tccggataaa
     1801 aacagtcacc aggccgatca gggatgtgat ccacagtaca atatatccgg ctgaggtgta
     1861 atctgccgta agattccgca cgcccttttt cagcatgacc catgcctccg aaaaagggac
     1921 ctgtgtatta tatttcgaca aatacttcac cgtccagaac cagaacctgt cgaagtcggc
     1981 aaacaaggca aaatacagga tcattaacag cagcagggcg gcaaatccgg ctgcaaaaag
     2041 ggccaggtgc ttcaccatcc cggcttttga aatatttcca tattccataa caaaaacaac
     2101 ggttgctccg aagagggcga aaaacaatcc cgattgcttg cagataaaag caaaggaaaa
     2161 gaataaccct gccaggaagt ataccgacca tcccctctta ttgtttttat aatgatggat
     2221 caaaatataa aaaccgatca aagcgaaaaa aacgacaaaa tgagtggcat gtgccgcctg
     2281 ggccagcaaa gtataagatg ttcctataat tccgaagacg gcggtggcca ttaccgcccc
     2341 tatttttgat aaaaaagatt tggcgatcag aaacaccagg aacatggatg ccgtgacgat
     2401 taaagtcaat ccaaggtgaa tcccgacgat ggattccccg aatatgccca tcagcaaggc
     2461 atacatgtaa taggtcccgg gaagtttcat gttgtaggcc agggtatagg gggcatgccc
     2521 gtcaaggatg agctttccga aataggcata ttccccttca tcccgctcaa gcggaaaaga
     2581 aagcaaatgc agcctggcaa ttagaacaac aataacggca atggccaggc agatataaac
     2641 aaacaggttg ttttccgtta aatttttact ggcctggttc gatttttcat ctctcataaa
     2701 tcattccggg gtattaagat gtttggaaat aataaaatca cttttatacg gatccatcga
     2761 ctgcaaatgt aacataaaga taccctttag cagtcatttg caaactttgc ctaactttat
     2821 gacttcattt ttccagatga gatgacggca tcaaaaaaca tatccaaaag ggaatttatc
     2881 aaggcctctt tctttggcgg atgcggtttt tgcatggccg cctcagggtt gaatgcgctg
     2941 gccggcagct ccttcggcaa tgatagtttg tcacatttag cagaaaatgg tgctttgcct
     3001 caggacactc cgtggaaatg gagtacgccg gctctgtact tcgtggatac ccccaaaggg
     3061 atcaaatgca aactgtgccc gcaaaaatgt gaaatagagc cgggtaaggc tggcgactgc
     3121 cggaccaatg tgaattacaa cggggtcctt tactccatca gctatggcaa tccgtgcgcc
     3181 gtccacatcg acccggtcga aaaaaaacct ttg
//
LOCUS       DAOQXS010000364         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31833_length_3205_cov_3.857778, whole genome shotgun sequence.
ACCESSION   DAOQXS010000364 DAOQXS010000000
VERSION     DAOQXS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31833_length_3205_cov_3.857778"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="priA"
                     /locus_tag="PKM34_09750"
     CDS             <1..1281
                     /gene="priA"
                     /locus_tag="PKM34_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNQ83915.1"
                     /translation="LPYHNLGLVIVDEEHDSSYKQYDPAPRYHARDAAIMLARLHGAK
                     TLLGSATPAIESYFNASNGKYGLVEMTERFGGVSLPEIVLANVREETRNKTMQSHFTS
                     ALINRIRDALALKEQVILFQNRRGFSLHLTCDSCDWVPMCKNCDISLTYHKRENKIKC
                     HYCGYSEALPQECPQCKSTRILMKGFGTEKVEEELGAIFPEATIRRMDLDSTRTKYAH
                     QRLISDFEERRTDILVGTQMVTKGLDFDNVSLVGILNADNMLNFPDFRSHERSFQLME
                     QVSGRAGRKAKQGQVIIQSYNPQHAIIQHVVHHDYHAFYRSQLTDRHKFQYPPFNRLI
                     ILKVKHRDYNVLNKAASDLAHDLRVDLGKRILGPEYPLVSKIQNWYIKHIMVKIERGN
                     NIAEMKKIILNRIENTGRIPAYRQVRVVIDVDPV"
     gene            complement(1285..1581)
                     /locus_tag="PKM34_09755"
     CDS             complement(1285..1581)
                     /locus_tag="PKM34_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83916.1"
                     /translation="MIMLRILKKIFLNRYFITLVAFAVWMIFFDTNSLKRQHALNQRI
                     TEIREMKAFYASEIARNNTLIEELINNPEAIEKFGREKYLMKRDSEDIYLVTRE"
     gene            complement(1749..2675)
                     /locus_tag="PKM34_09760"
     CDS             complement(1749..2675)
                     /locus_tag="PKM34_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNQ83917.1"
                     /translation="MIRVLSVNISEKKGTVKQPVDSICLDEWGVKNDAHAGPWNRQVS
                     LLAKESVDKFSKAAGREIRFGEFAENITTDGIILHETHPLDRFIIGETELEVTQIGKE
                     CHGDSCAIFREVGNCVMPKEGIFCRVIRPGIVRPGDPIIFKEKIYKPMVITLSDRASK
                     GIYEDKSGPRTIAILNDFFTQQNWKSEITHHIIPDDENELKSLLQKAKSDKTDIVITT
                     GGTGIGPRDITPEAVRSVIDKEIPGIMELIRIKFGMEKPNALLSRSVAGVAGSTLIYA
                     LPGSVKAVNEYMAEISKTLRHSVFMLHGIDSH"
     gene            complement(2759..>3205)
                     /gene="moaC"
                     /locus_tag="PKM34_09765"
     CDS             complement(2759..>3205)
                     /gene="moaC"
                     /locus_tag="PKM34_09765"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF008999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNQ83918.1"
                     /translation="SSFSHTDESGRARMVDVGHKPEQLRIATATGYIRLNPETIRLIR
                     ENEIKKGDVLTVAQIAGIQAGKKTADLIPLCHPLQITKIDVVTAIGDSGVTVTATAKC
                     VGQTGVEMEALTAASVALLTIYDMCKAVDKEMVIGEIRLVEKRKEE"
BASE COUNT          747 a          858 c          771 g          829 t
ORIGIN      
        1 ctgccctacc acaacctggg ccttgtcatt gttgatgaag aacacgattc atcgtacaag
       61 caatatgatc cggcgccgcg gtaccatgcc cgggatgcgg cgatcatgct ggccaggctc
      121 cacggggcaa aaacgttgct gggctctgct accccggcaa ttgaaagcta ttttaacgct
      181 tcgaacggaa aatacgggct ggtggagatg acggaacggt tcggcggggt cagcctgccg
      241 gagattgtgc tggccaacgt gagggaggag acgaggaata aaacgatgca gtcgcacttt
      301 acctcggccc tcatcaatcg catcagggat gcgctggcgc tgaaagagca ggtgatcctt
      361 ttccagaacc ggcgcggatt ctcgctgcac ctgacctgcg attcgtgcga ctgggtcccg
      421 atgtgcaaga actgcgacat ctccctcacc taccataagc gggagaacaa gatcaaatgc
      481 cactactgcg gttattcgga agcattgccg caggagtgcc cgcagtgcaa aagcacgcgt
      541 atcctgatga aagggttcgg aacggagaaa gtggaagaag aacttggcgc gatattcccg
      601 gaagccacca tccgccgtat ggacctcgat tccacgcgga cgaaatacgc ccaccagcgg
      661 ctgatctccg attttgaaga gcggcggacc gatatcctgg tcgggacgca gatggtgacc
      721 aaagggctgg atttcgacaa cgtcagcctc gtcggtatcc tgaacgccga taacatgctt
      781 aattttccag acttccggtc gcacgaacgg agttttcagc tcatggagca ggtcagcggg
      841 cgggccggga ggaaagcgaa gcaaggccag gtcattatcc agagctataa cccgcaacat
      901 gcgatcatcc agcatgttgt ccatcacgat tatcacgcct tctaccgctc acagctcacc
      961 gaccggcaca aattccagta cccgcctttc aaccggctga tcatcctgaa ggtcaagcac
     1021 cgtgattaca atgtgctgaa caaggctgcc agcgacctgg cccacgatct gcgggtggac
     1081 ctgggaaaaa ggattctcgg ccccgaatac ccgctggtct caaaaatcca gaactggtat
     1141 atcaagcata tcatggtcaa gattgagcgg gggaacaata tcgctgagat gaagaaaatt
     1201 attctaaaca ggattgaaaa taccggcagg atcccggctt acaggcaggt acgggtggtt
     1261 attgatgttg acccggtttg atcttcactc tctcgtaacc aggtagatat cctcgctgtc
     1321 gcgtttcatc aggtatttct cccggccaaa tttttcaatg gcttcgggat tattgatcag
     1381 ctcctcgatc aaggtgttgt tcctggcaat ttccgacgca taaaacgctt tcatctccct
     1441 gatttcggta atgcgctggt tcaatgcgtg ttgccgcttc aggctgttcg tatcgaagaa
     1501 aatcatccat acggcgaatg ctaccagtgt gatgaaatag cggttgagga atattttttt
     1561 caggatgcgg agcatgatca tttaatcagg atttaaagat aaggttgata atgcagatga
     1621 ttccttaaaa tagcaggaat ttttaacttg attttgttga taaccgatat gccatataaa
     1681 cactataatc cgttgaagcg ccattgcccg cgttttactt ccgacgactg aaaatgtcag
     1741 gtgataaatt agtgactatc aatgccatgt agcataaaaa ctgaatgcct caaggttttt
     1801 gagatttcag ccatatattc attgaccgct ttaacgctac ccgggagagc atagatcagg
     1861 gttgaaccgg ccaccccggc cacgctccgg ctcagcaatg cattcggttt ttccatacca
     1921 aatttgatgc ggatcagttc cataatgccc ggaatctctt tatcaatcac cgaacgcacc
     1981 gcctccggtg tgatatccct cggcccgatc cccgtgccac cggtggtaat aacgatgtcg
     2041 gtcttgtcgc tttttgcttt ttgcagtaat gatttcagtt cattctcatc atcggggatg
     2101 atatgatgag tgatttcgct tttccagttt tgttgtgtaa aaaaatcatt caggatggcg
     2161 atggtgcgcg gcccgctttt atcttcataa atgcctttgc tggccctgtc gctgagggtg
     2221 atcaccattg gcttataaat tttttcttta aagatgatcg ggtctcccgg acgaacgatt
     2281 cccgggcgga tcacgcggca gaaaatacct tctttcggca tcacgcaatt gccaacctcg
     2341 cggaagatgg cgcaactatc gccatggcat tctttgccga tctgggtcac ttccagttcg
     2401 gtttcgccaa tgatgaacct gtcgagggga tgggtttcat gcaggatgat cccgtcggtc
     2461 gtgatatttt cggcaaattc gccgaaccgg atctcccgtc cggcggcttt tgagaatttg
     2521 tcaacgctct cttttgccag caggctcacc tggcggttcc acggaccggc atgcgcatcg
     2581 ttcttcaccc cccattcatc caggcagata gaatctaccg gctgcttcac cgtccccttt
     2641 ttttccgaaa tattaaccga caatacccta atcataccaa tttcctattt ctgattcaaa
     2701 atgcaccatt tctttttcct ttttccctct tcactcttca ctcttcaccc ttcactcttc
     2761 actcttcctt cctcttctcc accagcctga tctccccgat aaccatctct ttatcaaccg
     2821 ctttgcacat gtcgtaaatt gtaaggaggg caacggatgc agcggtcagc gcttccattt
     2881 ccacgccggt ctgtccgaca cactttgccg tagcagtaac tgtaacaccc gaatcaccga
     2941 tagccgtcac gacatcgatc tttgtgatct gaagcgggtg gcatagcggg atcagatcag
     3001 cggtcttttt tcctgcctgg ataccggcga tctgcgcaac cgtcagcaca tcgccttttt
     3061 ttatttcgtt ttcccgtatc agccggatgg tctccgggtt caggcggata tagccagttg
     3121 ctgtagctat ccttaactgt tccggcttgt ggcccacatc gaccatcctt gcccggcccg
     3181 attcatcggt atgcgaaaag ctgct
//
LOCUS       DAOQXS010000365         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31908_length_3200_cov_3.247695, whole genome shotgun sequence.
ACCESSION   DAOQXS010000365 DAOQXS010000000
VERSION     DAOQXS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31908_length_3200_cov_3.247695"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKM34_09770"
     CDS             complement(<1..668)
                     /locus_tag="PKM34_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83919.1"
                     /translation="MKKLIMMIIAFSHTYLAILNASICRAQDSRKEAISLEDYIGDFF
                     EGEDEYMTGLGEWQEAVSRWLETPVCLNSDETEWLAEYRIIDPYQLSKLKEYRYIYGN
                     LLSVYELAFIEGWDEQSARKVFPLVTVLSGNNPSFIPGKFDLRSFRQSLSVKYRSNSG
                     KSRGYDKLYPGKSDSAVTHFRGSPAGIALRYDLQYRNRLELGIRMEKDPGEPMLQQTS
                     VLSQ"
     gene            complement(665..1501)
                     /locus_tag="PKM34_09775"
     CDS             complement(665..1501)
                     /locus_tag="PKM34_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83920.1"
                     /translation="MKIKMIGTLMAFLIIGGLGNLSWSRVFGQTGPAGGRCIAMGGVT
                     SVSEEIWAAANNPALLAGDCLPAISICLENKYLTGQMQYNSVAGILPAGHGGLGFTGK
                     LLLMSTYCDALMSLGYGRPFGEKVKIGINLVYHIQSAGEGFDPVHKVSYGLGTAVDLS
                     AKVSLSFSTFNPFRLYYKSSGYATLPSVFNLGMVYRYAPAFLVAIELEKEMDFPLCVK
                     TGMEYNFGETVFIRGGIRLLPFGYSFGTAWRKKRLLIEVSSSYHQYLGFSPVISFQYN
                     LK"
     gene            1587..2240
                     /locus_tag="PKM34_09780"
     CDS             1587..2240
                     /locus_tag="PKM34_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNQ83921.1"
                     /translation="MEKIIFDFLNELKTNNDREWFQSNKSRYEKAKKSFESFIDELIP
                     VIRGIDPNIDLITAKDCVFRIYRDVRFSHDKSPYKTNMGAYITRGGKSSPMAGYYVHC
                     EPGGSFLAGGIYMPQPDVLKKIRDEVFYKYDDFRKIIEDKEFVKTFGQIDDDQKMKSP
                     PRGYPKEFPGIELLKFRNYAVAHFVSDELALSEKYPEYAKKVFKTLYPLNSFFNNVF"
     gene            complement(2565..>3200)
                     /gene="pyrF"
                     /locus_tag="PKM34_09785"
     CDS             complement(2565..>3200)
                     /gene="pyrF"
                     /locus_tag="PKM34_09785"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNQ83922.1"
                     /translation="PNLAFYESTGLKGWISLEKTISYLHMKKDRVFLIADAKRGDIGN
                     TSRQYAKAFFEILDVDGVTVAPYMGSDSVQPFLTYPGKWVVLLALTSNKGASDFQYLR
                     HNDSYLFEEVLRTSSQWGSEENMMFVVGATQAAQLETIRGIVPGHFLLVPGVGAQGGS
                     LQEVVRYGKNNDIGLLVNASRSIIFASSKEDFAERAADEALKMQKEMASLM"
BASE COUNT          869 a          788 c          681 g          862 t
ORIGIN      
        1 tgttgtgata aaacagatgt ctgctgaagc atgggctcgc caggatcctt ttccatacgg
       61 atgcccagct ccagtctgtt tcggtattgc agatcataac gcagcgcaat tcccgcggga
      121 gatccccgga aatgcgttac cgcggaatcg ctctttccag gatacaactt atcatacccc
      181 ctggatttgc cggaattcga tctgtattta acggatagac tttgccggaa tgatctcaga
      241 tcgaattttc cgggaataaa ggagggatta ttgcctgata atacggtaac cagcgggaac
      301 actttgcgcg ctgactgctc atcccatcct tctataaaag ccagctcata tactgaaagc
      361 aggttgccgt agatgtaacg gtattctttc agcttactga gctggtaggg atcaatgatc
      421 cggtattctg ccagccattc cgtttcatca ctattcaggc agaccggggt ttcaagccac
      481 ctcgaaacag cctcctgcca ctccccgaga ccggtcatat attcatcctc cccttcaaag
      541 aagtcaccta tatagtcctc cagggatatg gcctctttcc ggctatcctg tgccctgcat
      601 atcgaagcgt taagtatggc aagatatgta tggctaaatg caattatcat catgataagc
      661 ttcttcattt cagattgtat tgaaaagata taacaggtga aaatcccaga tactgatgat
      721 aagatgagga aacttcgata agtaatctct ttttcctcca tgctgtaccg aatgaatagc
      781 cgaatggcag caagcggatg ccgccccgta taaatacggt ttccccgaaa ttgtattcca
      841 ttcccgtttt gacgcataag gggaaatcca tctccttctc cagctcgatt gcaaccagga
      901 aagccggagc atagcggtaa accatcccca gattgaaaac cgagggtaat gttgcatacc
      961 ctgaactttt ataatagagc cgaaatggat tgaaggttga gaaagagaga gataccttag
     1021 ccgacaggtc cactgctgta cccaaaccat agctgacttt gtgcaccgga tcaaatcctt
     1081 cgcctgccga ctgaatatga tagaccaggt tgatcccgat ctttaccttt tccccgaatg
     1141 gtctgccgta accaaggctc attaatgcat cgcagtaggt tgacatgagc aatagtttcc
     1201 cggtaaagcc cagtcctcca tggcctgccg gcaggattcc ggccaccgaa ttatactgca
     1261 tctgccccgt taagtattta ttttccaggc atatcgagat ggctggaagg caatcccctg
     1321 ccagcagtgc cggattattg gctgcagccc aaatctcctc gctgactgat gtgaccccac
     1381 ccatcgcaat acatcttccc cctgccggcc ccgtctgtcc gaatacccgg ctccatgaca
     1441 aattacccag acctccgata atcaaaaagg ccatcaacgt cccgatcatt tttattttca
     1501 taacacctcc tatattgctt aatccgtttt ttgccccaat tatacccggt cgatgcaaga
     1561 aaaaaataac tttgttaaaa aaaagcatgg aaaagattat ttttgacttc cttaacgaat
     1621 tgaaaaccaa taatgaccgt gaatggttcc agtcgaataa atcccgctat gaaaaagcca
     1681 aaaaatcgtt cgaatcattc attgatgaac ttattccggt tatcaggggt attgatccga
     1741 atatcgatct cataacagcg aaggattgtg tattccggat ttatcgcgat gtgaggtttt
     1801 cacacgacaa atcgccctat aaaaccaata tgggtgccta tattaccagg ggaggaaaga
     1861 gttcaccgat ggccgggtat tatgttcatt gcgagcccgg cggttctttc ctggcgggag
     1921 gtatctacat gccgcaacct gatgtgctga agaagatcag ggatgaggtc ttttataaat
     1981 atgatgattt caggaaaatt attgaagaca aggaatttgt aaagactttc ggacaaattg
     2041 atgatgacca gaagatgaaa agtccgcccc ggggatatcc aaaagaattt ccgggaatag
     2101 aactgctgaa attccggaat tacgctgtgg cgcattttgt ttcagacgaa ctggccttaa
     2161 gtgaaaagta cccggaatat gccaaaaagg tttttaaaac actttacccg cttaactcat
     2221 tctttaacaa tgttttttaa gaggtttttt ttaatgatga ccaggtaaga cattgccgtt
     2281 aacttgagaa tgattccgaa aactgattca aatcaaccgt taacttattt tatttgcatc
     2341 acgtgtagaa aaacttgtac ttttatctcg gattaattgg ttaattattt cctacttcgg
     2401 aggcttttga agatttggtt aattggttta ctttacttca aaagtccttt ctcacctcaa
     2461 actagtaaga cttggcaaaa gcaaccccga cgagctcggg gttgctcttt tatggcaacc
     2521 tgtaatttca ggatggttat aatttctgga aacagtcaag attatcacat gagagaagcc
     2581 atctctttct gcatcttcag cgcttcgtct gccgcccttt cggcaaagtc ctctttactg
     2641 gatgcgaaaa taatgcttcg cgaagcattg accagcaggc cgatgtcgtt gttcttcccg
     2701 tagcggacca cctcctgcag acttcctccc tgggcgccga cacccggcac aagcaagaaa
     2761 tgccccggga ctataccgcg tatggtttcc agttgtgcgg cctgcgtcgc gccaacaaca
     2821 aacatcatat tttcctccga tccccattgt gaggaggtcc gtagcacctc ctcaaaaaga
     2881 taagagtcgt tgtgccggag atactggaaa tcactggccc ctttgttgga tgtcagggct
     2941 aacagcacaa cccattttcc cgggtacgtc agaaatggct gcaccgaatc tgatcccata
     3001 tagggcgcta ccgtcacgcc atccacatcc aggatctcaa aaaaggcttt cgcatactgc
     3061 cgggaagtat tgccgatatc gccccgtttg gcatcggcaa tcaggaacac ccggtccttt
     3121 ttcatgtgca ggtacgaaat cgtcttctcc aggctgatcc accctttcaa accggtactt
     3181 tcataaaagg ccagattggg
//
LOCUS       DAOQXS010000366         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_31996_length_3194_cov_3.397897, whole genome shotgun sequence.
ACCESSION   DAOQXS010000366 DAOQXS010000000
VERSION     DAOQXS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31996_length_3194_cov_3.397897"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="PKM34_09790"
     CDS             <1..1857
                     /locus_tag="PKM34_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83923.1"
                     /translation="NNNGSPGGKSGSIGDGGATCTQCHTGGSATTVSGWITTNVPADG
                     YTPGQTYTITATGTHTGVVKFGFELTVESSTGAKVGTLQLTEPTRTKFTNANQAVTHT
                     AAGNVPSGNTNSWTMNWVAPTGVQGNIGIYAAFNAANGNGNNSGDVIYKSSTFISQYV
                     PPPTLVSIVPDEAEQGQTVQTTITGSNTQFSGSPSVSLSFSNNPNETISASNVVVVNA
                     TVIQAQFAVPGNASVGLWDLHVNLLSLDNAFTVIQAEPSIVFMEPNFAYQGDSFTGTI
                     YGENTTWSGTPSVTLTHVNNPNEIITGTNVTVVSPTQLTADFAIAEAGVIGNYNVNVD
                     DLMQAAGFTVLAALVPEIAGISPVEGEQGTMVTTTITAENTHFGQPAPVVSLTLHGNP
                     GESIPATSVTVINGTTLEADFDIPYEATPGLYDLQVDDLTLVNAFTVIDVVPFLVSIS
                     PNTADQGETVVTTITAADSRFTLDEPSVSLSFSGDPLETIAASGVTVISNFVVEAEFI
                     IPSNASVGTWDVHADEMTLSGAFTIDQAIGIADNLAGMIRIYPNPADQKFFVENGRGA
                     EITIFRTSGEQVLSLVAGNIRQEVNIETLTQGLYLVNIRMNGMVKTEKLLVK"
     gene            complement(1861..>3194)
                     /locus_tag="PKM34_09795"
     CDS             complement(1861..>3194)
                     /locus_tag="PKM34_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ83924.1"
                     /translation="ERIVETMGPFVGNTHTETSETGTLMTNAYHFAHQKIKEHVNASA
                     DDVIITAGFGMTAVVNKLQRMLGLKSCGQGSKHNCIDESDRPVVFITHMEHHSNHTSW
                     FETMSDVVQVPPANDLMIDLNRLRELLEQYKERKIKIGTFTACSNVTGIATPYHQMAR
                     LMHEYGGYCFVDFAASAPYVDIDMHPEDPMEKLDAIFFSPHKFLGGPGSSGVLIFDRN
                     LYSGHRPPDQPGGGTVDWTNPWGEYKYIDDIEAREDGGTPGFLQAIKAALAIGLKEKM
                     GTGNIKKRESELLSVAFAGFDRIPELHLLADENRERLGIISFYIENIHFNLAVKLLND
                     RFGIQVRGGCACAGTYGHYLLDVSYDKSHLITSLISSGDLSLKPGWIRLSLHPTMTRG
                     ELEYILDAIGQVQKNHKKWCDDYVYNRHTNEFRHRNEPEDKTVLVREWFEL"
BASE COUNT          795 a          840 c          781 g          778 t
ORIGIN      
        1 aacaacaatg gctcgccagg cggtaagtcg ggatcgatcg gcgacggagg agctacctgt
       61 acccagtgcc acaccggtgg tagtgcaaca actgtctcag gctggatcac gaccaatgtt
      121 ccggcggatg gttacactcc cggacaaacc tacacaatta cggcaacagg tacacatacc
      181 ggcgttgtaa aatttggctt tgagctgacc gtagaaagca gcaccggagc taaagtagga
      241 accctgcaac tgactgagcc gacccgcaca aagttcacta atgcgaatca agccgtaacc
      301 cacacagcgg caggaaatgt tccgtcagga aataccaact catggacaat gaactgggtg
      361 gctccgacgg gggttcaggg caatatcggc atctatgccg cattcaatgc agccaacgga
      421 aatgggaaca attcaggtga tgtgatttac aaatcaagca cctttatttc acaatatgta
      481 ccgcctccaa ctctcgtaag catcgtcccc gatgaggccg aacaagggca aacggttcag
      541 actacaatta ccggtagcaa tactcaattt tcagggtcgc catctgtttc cctctccttc
      601 agcaataatc ccaacgaaac tatttccgct tcaaacgtag tggttgtcaa tgcaactgtt
      661 attcaggcac aatttgcagt cccgggaaat gccagcgtcg ggctttggga tctgcacgta
      721 aacctgcttt cacttgataa cgcgtttacg gtcatccagg ccgaaccctc tattgttttc
      781 atggagccca actttgctta ccagggtgat tcattcacag gtacaatcta tggcgaaaac
      841 accacctgga gcggaacacc atcggttacc ctgactcatg tcaataatcc gaacgagatc
      901 attaccggta cgaatgtcac tgtggtcagc ccgacccagc tgaccgccga ctttgcaatt
      961 gctgaggcag gtgtgatcgg aaactataat gtcaacgtcg atgacctcat gcaagctgca
     1021 ggtttcaccg ttctcgccgc tctcgttccg gaaattgccg gtatcagtcc ggttgaaggt
     1081 gagcagggaa cgatggtcac caccacgata accgctgaaa atacccattt cggacaaccg
     1141 gctccggtgg ttagcctgac gttgcatggc aatccgggcg aaagtatccc ggcaaccagc
     1201 gttaccgtta ttaatggaac caccctggaa gccgatttcg atattcctta cgaagcaacc
     1261 ccggggctgt atgaccttca ggtcgacgat ctgacgctgg ttaatgcgtt taccgttatc
     1321 gacgtggtgc ctttcctggt cagtatttct cccaatacag ccgatcaggg ggaaaccgtc
     1381 gtgacaacga tcacggcagc cgacagccga tttaccctgg atgaaccatc tgtttctctg
     1441 agtttttcgg gtgatccgct tgaaaccatt gctgcttcgg gcgttacagt tatcagtaat
     1501 ttcgtcgtcg aagctgaatt cattattccg tcaaatgcat ctgtcggaac atgggatgtc
     1561 catgccgatg aaatgaccct cagcggagcg tttaccatcg accaggccat cgggattgcc
     1621 gataatcttg cgggcatgat ccgaatctat ccgaatccgg ccgatcagaa gttcttcgtt
     1681 gaaaatggcc gcggagctga aattaccatc ttccgcacaa gcggcgaaca ggtgttgagc
     1741 ctggttgccg gcaatatccg gcaggaagtc aatattgaaa ctctgacaca gggattgtac
     1801 ctggtcaata taaggatgaa tgggatggtg aaaacggaga aactgctcgt aaagtaaaaa
     1861 ttacagttcg aaccattccc tgacaagaac ggttttgtcc tcaggttcat tccggtgcct
     1921 gaactcattg gtgtggcggt tataaacata atcgtcacac cattttttat ggtttttctg
     1981 aacctgcccg attgcatcca ggatatactc cagctcgcca cgggtcatcg tcggatgcag
     2041 ggagagcctg atccatccgg gcttcaggga taagtcgcct gagctgatga ggctggtgat
     2101 cagatgggat ttatcatagg aaacatccag gaggtaatgc ccgtaagtgc ctgcacaggc
     2161 gcagcctccg cgcacctgga taccgaaccg gtcgttcagc agtttgacgg ccaggttgaa
     2221 atgaatattc tcaatgtaaa aagagatgat tccgagcctc tcccggttct cgtcggccag
     2281 caggtgaagc tcgggaatac ggtcaaaacc tgcaaaagcc acagataaca gttcgctttc
     2341 cctttttttg atatttcctg tgcccatctt ttctttaagc ccgattgcca atgccgcttt
     2401 gatagcctgg agaaacccgg gtgtcccgcc atcctcgcgg gcctcaatat cgtcgatgta
     2461 tttatattcc ccccagggat ttgtccagtc gaccgtccct ccgccgggct gatcgggcgg
     2521 acggtgaccg ctgtaaagat tcctgtcgaa aatcaaaacg cctgaacttc ccgggccgcc
     2581 gagaaattta tgcggtgaaa agaaaatggc atccagcttc tccatcggat cttccgggtg
     2641 catatcaata tcgacataag gcgctgatgc agcaaaatcc acaaaacaat agccgccgta
     2701 ttcgtgcata agcctggcca tctggtgata tggagtggcg atgcccgtca cattggagca
     2761 ggcagtaaat gttccgatct tgattttccg ttctttatat tgctccagta actcccgtaa
     2821 ccggttcaaa tcgatcatca ggtcgttggc cggcggaacc tgaacgacgt ccgacatggt
     2881 ctcgaaccag gaagtatgat tggaatgatg ctccatatgg gtgatgaaaa ctaccggccg
     2941 gtccgattca tcgatgcagt tgtgcttcga tccctggccg cagcttttta acccgagcat
     3001 ccgttgcaat ttattcacaa ccgctgtcat tccgaatcca gccgtgatga tcacatcatc
     3061 tgccgatgca ttgacatgct ccttgatctt ctgatgggca aaatggtagg cattggtcat
     3121 caatgtgccg gtttcactgg tctcggtgtg tgtattaccg acaaacggtc ccatcgtttc
     3181 tacaatacgc tctt
//
LOCUS       DAOQXS010000367         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32103_length_3186_cov_4.842542, whole genome shotgun sequence.
ACCESSION   DAOQXS010000367 DAOQXS010000000
VERSION     DAOQXS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32103_length_3186_cov_4.842542"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PKM34_09800"
     CDS             <1..966
                     /locus_tag="PKM34_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ83925.1"
                     /translation="DIYGLKRMHNLRNILIIVVSISILLMLVSGWIYAGRALYPISRI
                     ISKGNEISITSLNLRLDEGNQKDEIAQLAGTFNKMLDRLETAFKSQKRFIANASHELR
                     TPLTAITGQLEVLLMQDRTTEEYKRAVNSVLEDMKNLNSISNRLLLLAQASNDLSELN
                     VKPIRIDETIWQCREELIRRNENNHIIVHLDNSLEDDAALTFMGDEQLVRTMIINLMD
                     NGCKYSPDHTVEVTIGADQLQLLLTFTDRGIGIPEEDLAHVFEPFRRGQNAYPVKGHG
                     IGLSLVERIVILHNGSIEVDSQPGEGTTFRVFLPQKTDPEFLTTF"
     gene            1188..2057
                     /locus_tag="PKM34_09805"
     CDS             1188..2057
                     /locus_tag="PKM34_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83926.1"
                     /translation="MKMNRERVIAATTTILLVASLIGTGYYYRTSGTLKSGLNNEKLK
                     SESLLSEKLALAKEIEKLKSDISQWQGKSQESDKLLAEAREKIEAMEKTMSWLRKENA
                     TVASLKKELNELKQLRTDLEAQLAELERKNKDKDNQIEALHAEVIALKSERDGLKSDV
                     AALKANITDDFRVETLRGKKNQKLTLNARKTKKLMVSFEIPQSMSDNVRFTIETPNGK
                     EIKSDDKSLTYNVVDDGRNMIANLSPYTGEFEISRRIEMAYKPDQKLEPGIYKIKIYH
                     KDTYTGSCQVRLK"
     gene            2226..>3186
                     /locus_tag="PKM34_09810"
     CDS             2226..>3186
                     /locus_tag="PKM34_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83927.1"
                     /translation="MRLLTVSILASLLLLSCATGKKAYERGDYFNATIQAVNRLRSNP
                     GSEKAQEALRRSYPAALGYFQEKIENALRSNRQFRYSEVVDYYVMMNHMADEIRRCPA
                     ALSLFPQPKSFESELAEAKYKAAEEHYQAGLDQEKLNTRTSWKSAYLNFNEADCFVPG
                     FKDAKERMRIAYLNATWKVIVEPIPVPNAYQLTSDFFRNQVIEYLRNERPNEFVEYYT
                     PESAEKAGIKKPDQVLRMSFDQFVIGQVYDKETITELSRDSVVTGTVRLPDGTEHKVY
                     GTVKAKLRTFRREVISNGVLDVSVIDWPLNQLATERKFPGQFIW"
BASE COUNT          969 a          757 c          755 g          705 t
ORIGIN      
        1 gatatttatg gcctgaagag aatgcataac ctgcgcaata tcctcatcat cgtagtgagc
       61 atcagcattc tccttatgct ggtgtcgggc tggatctatg caggcagagc gctttatccc
      121 atctccagga ttatttcaaa aggcaacgaa atttcaatta ccagcctcaa tctgcgcctg
      181 gatgaaggga atcaaaaaga tgagatcgcc cagttggccg gaaccttcaa taaaatgctg
      241 gacaggctgg aaacagcatt caaatcgcag aaacggttca tagccaatgc ttcccatgaa
      301 ctgagaacgc ccctcacggc cataaccgga cagcttgaag tgcttctgat gcaggaccgg
      361 acgaccgaag aatataagcg tgcggtcaac tccgtattag aagatatgaa aaaccttaac
      421 tctatctcaa acaggttatt gctgttggct caggccagca acgacctgtc ggaattgaat
      481 gtcaaaccga tccgaattga tgaaacgatc tggcagtgcc gcgaagaatt gatcagaaga
      541 aatgaaaata accacatcat agtccatctt gataactccc tggaagatga tgcagctttg
      601 accttcatgg gcgatgaaca attggtcagg accatgataa tcaacctgat ggacaatggc
      661 tgcaaatact ctcccgatca tacggtggaa gtgacgattg gagcggatca actgcaactg
      721 ctgctgactt tcaccgaccg gggtattggt atacctgagg aagacctggc gcatgttttc
      781 gaacccttcc ggcgcggcca gaatgcttat cccgtcaagg gccacggcat aggtctctcc
      841 cttgtagaaa ggattgtgat acttcataac ggatccattg aagtcgattc acagcctggc
      901 gaaggcacaa ctttccgggt ctttctgccc caaaaaactg atcctgaatt tttaaccaca
      961 ttttaatccc ggtttcattc cgtttttatc ctgcagtaat agttttgctt ccagatttag
     1021 tgttaattgg gactaaggag ccgcttatcc tgaaaccagc atgaaatgaa tgaagcggta
     1081 aaatccatta aaaccaacaa gaactacctg atcgttggca tgctggtttt cttttacatt
     1141 gaatcagaaa aacaattatt gtataatcat ttaaaacaag catggtcatg aaaatgaaca
     1201 gagagagagt gattgccgct actacaacca tacttcttgt agcatcactg ataggaacgg
     1261 gatattatta ccgcaccagc gggacgttga aaagcggtct gaataacgaa aaactgaaat
     1321 cggaatcctt gctgtctgag aaactggcac tggccaagga aatcgaaaaa ttgaaaagcg
     1381 atatctcaca atggcagggg aaaagccagg aatcggacaa attgctggca gaagccaggg
     1441 aaaaaatcga ggcgatggaa aaaaccatga gctggctcag aaaagaaaat gcgacggtag
     1501 cctctttgaa aaaggagctg aacgaactca aacagctcag aacggacctg gaagcccagc
     1561 tcgcggaact ggaaaggaaa aacaaagata aagacaatca aattgaagca ttgcatgccg
     1621 aggtcattgc cctcaagtcg gaacgcgacg gactgaaatc ggatgtagcc gctttaaagg
     1681 caaatattac cgacgacttc cgggtcgaga ccctcagggg caagaaaaat caaaagctga
     1741 ctctgaacgc caggaagacc aaaaagctca tggtcagctt tgagatcccc cagtcgatga
     1801 gtgataacgt aagattcacc attgaaacac caaacggcaa agagattaaa agtgatgaca
     1861 aaagcctgac ctacaatgtt gtcgatgacg gacggaacat gattgccaac ttatcgcctt
     1921 ataccggtga atttgagatc tccaggagga tagaaatggc atacaaaccg gatcaaaaac
     1981 tggaaccggg tatctataag atcaaaatct atcataaaga tacctatacc ggaagctgcc
     2041 aggttcgtct gaaatagaag aagcgtatat ctcaatatat tttcatggta aattttctca
     2101 ccccgggcta ccgctgaatc ccagccggtg gcccggtttt ttcatggcac gattttttca
     2161 atgttcaacc caaggatttc acccaaaata agaaaatttg cttactttgt aaaaattgcc
     2221 gaattatgag actattaacc gtttccatcc tggccagtct tttgttgctc tcttgtgcaa
     2281 cggggaaaaa agcgtacgaa agaggagact atttcaatgc cactatccag gcggtgaacc
     2341 ggctgcgcag caatcccgga tcggagaaag cacaggaggc cctccggcgc agctaccccg
     2401 cagcgctcgg atatttccag gaaaagatcg aaaatgcgct tcgatccaac aggcagttca
     2461 ggtactccga agttgttgac tattacgtca tgatgaacca tatggccgat gagatcaggc
     2521 gatgcccggc cgcgctgagc ctgtttcccc agcccaaatc ttttgagagc gaactggccg
     2581 aagcaaagta caaagccgcc gaagagcact accaggccgg gcttgaccag gaaaagctca
     2641 acacaaggac atcctggaaa agcgcttatc tcaactttaa tgaagccgat tgttttgtcc
     2701 ccggcttcaa agatgcaaaa gaacggatgc ggatcgccta cctgaatgcc acctggaaag
     2761 tcatcgtcga gccgatcccc gttcccaatg cctaccagct tacctccgat tttttccgca
     2821 accaggtcat cgaatacctg aggaatgaac ggccgaatga atttgtagag tattacacgc
     2881 ctgaatcggc cgagaaagcg ggaatcaaaa agcccgacca ggtattgcgg atgagcttcg
     2941 accagttcgt catcgggcag gtttacgata aggagaccat tacggagctg tcgcgcgaca
     3001 gtgtagtgac cggcaccgtc agactgcccg acggaacgga acacaaagtc tacggcacgg
     3061 tgaaggcgaa gctgcgcaca ttccgcaggg aagtgatctc caacggggtg ctcgacgtct
     3121 cggtgatcga ttggccctta aaccaattgg ccactgaacg caagtttccc ggccagttca
     3181 tctggt
//
LOCUS       DAOQXS010000368         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32121_length_3185_cov_3.438339, whole genome shotgun sequence.
ACCESSION   DAOQXS010000368 DAOQXS010000000
VERSION     DAOQXS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32121_length_3185_cov_3.438339"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKM34_09815"
     CDS             <1..1140
                     /locus_tag="PKM34_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83928.1"
                     /translation="SNRFNYLTYALMGIGVITIIVGLFVNPVKTWANYLMNNYYFLSL
                     AIGATFFVALQYITQSGWSSAFVRIPQALSNVIPVIAVLMIPILVFGLPQLYHWAHAG
                     AADHDPIIAHKSPYLNLPFFIIRYVVFFAAWIFLTQYLKKLSLKEDRIGGMANFYKSE
                     LFSKIYIFTLAITFSLATFDWIMSVDVHWFSTIFAVRNFAMSFYHGTVVITLVIIILN
                     KLGYLPFLNKSHLKDFAKYIFILSIIWTYMWFAQYILIWYANIPEETVYYLPRTKGEF
                     TPLFYLELFLNFAIPFALLLSNYLVSHKNTLLAICIVIIFGHWVDLYLQIIVGTYGKL
                     EIGMIEIGTFLGFAGMFAFIVARALKSAPLVAKNHPYLEESLSHH"
     gene            1277..>3185
                     /locus_tag="PKM34_09820"
     CDS             1277..>3185
                     /locus_tag="PKM34_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNQ83929.1"
                     /translation="MMRKFFDFIFSMQFAGTLMLIFAAAVGTATFIENDFGTLAAKSV
                     VYSAKWFEALLALTAVSLIGSVFKYKLYERKRYSVLFFHLSFVVILIGAALTRYIGYE
                     GTMMIREGEASNQIISESPYVQVAVNKNGQSWNFDEEYLFTEYKKNRFRESFHFNDES
                     FKLQFKEYIPNAAEAVVEAKNGIPMMTFVTVDRGSRRTSLLKAGEVKTVRGLALSFNA
                     PTDDSTAIRINYSPETGLTVRTPFPSTVMNMMAGTTDTLRKDSTYTLFQMQLYDFMGM
                     QFVVRQFYPSGKTQLVSSPHESSGVAAIVMNLSYNGKTEEVGMLGGKGMVGQAVTTSI
                     DGTEFSLSYGSKYIGLPFYIKLRDFILERYPGSNSPSSFASEVTVIDPSENLEMPYRI
                     FMNNILNYGGYRFFQSSYDKDEKGTVLSVNSDYWGTLFTYIGYAILAAGLLYNFFSRN
                     SRFRKLARMTSELKKKSAMVVIAILIMVGFSAENAGAQHDGNFNPLNISKEHAKKFGE
                     LIVQDQDGRMKPVNTLSSELLRKIYRKDTYKGLNSEQVFLGIISNPEYWQGIPLIKVS
                     DDELKTAIGADGKYASFNDFLDNRQGFYKLGQHIEAANNKKPVERTSFDKDVIKVDER
                     VTQGQAVGIIAF"
BASE COUNT          820 a          781 c          730 g          854 t
ORIGIN      
        1 tcaaacaggt tcaattacct gacctatgcc ctgatgggta tcggtgtgat cacaatcatc
       61 gtcggattat ttgtcaaccc ggttaaaacc tgggccaact acctgatgaa caattactat
      121 ttcctgtcgc tggccatagg agcgactttc ttcgttgctc tacagtacat cacacaatcg
      181 gggtggtcga gtgcgttcgt gcgcatcccc caggcgttat cgaacgtcat cccggttatt
      241 gccgtgctca tgatccctat cctggttttc ggacttcccc agctctatca ctgggcccat
      301 gcgggagccg ccgaccacga tccgatcatc gcacataaaa gcccttactt aaacctgccc
      361 tttttcatta ttcgctatgt cgtctttttc gctgcctgga tcttcctgac ccagtatctt
      421 aaaaaactga gcctgaaaga agaccggatc ggcggaatgg ctaacttcta taaaagtgag
      481 ctgttctcaa agatttacat ttttaccctg gccattacct tttcactggc cacgttcgac
      541 tggatcatgt cagtagatgt ccattggttc agtacgatct tcgccgtcag gaactttgcc
      601 atgtcgttct atcacggaac ggtggtgatc acactggtca ttatcatcct gaacaagctg
      661 ggatacctgc ctttcctgaa taaatcgcac ctgaaggatt tcgccaaata catttttatc
      721 ctcagcatta tctggaccta tatgtggttt gcccagtata tcctgatctg gtatgccaat
      781 atcccggaag aaacagttta ttatttaccc aggaccaaag gcgaattcac tcccctgttt
      841 tacctggagc tgttcctgaa ttttgccatt ccgtttgccc tgctcctgtc gaattacctt
      901 gtcagccaca agaacaccct gctggcgatt tgtatcgtga tcatattcgg gcattgggtg
      961 gatctttatt tacagatcat cgtcggcacc tatggcaagc tggagatcgg catgattgaa
     1021 atcggcacat tcctgggatt cgccggcatg tttgctttta ttgtcgcccg ggccttgaaa
     1081 tcagccccgc ttgtggcaaa gaaccatcca taccttgagg agagcttaag tcatcattga
     1141 tttcaagcaa ggctatattt atttaaactc attctttata ataaggatga tttggttatt
     1201 tttgcatcgc agactatagt acgataggac tgttggacga gaacaatcga acaattgaac
     1261 aattgaaccc tgatttatga tgcgcaaatt ctttgatttc atattctcca tgcagtttgc
     1321 cggcacgttg atgctgatct ttgcggcggc ggtcggaacg gctactttta ttgaaaacga
     1381 cttcggtacc cttgccgcta aatcggtcgt atacagcgca aaatggtttg aagcgctgct
     1441 ggctctgaca gccgtaagcc tcatcggcag cgtattcaaa tacaagctat acgaacggaa
     1501 acggtacagc gtactgttct tccatctttc ttttgtcgtt atcctgatcg gtgcagccct
     1561 tacacggtac atcggttatg aagggaccat gatgatccgc gaaggcgaag catccaacca
     1621 gatcatctcg gaaagtcctt acgttcaggt ggctgtgaat aaaaacgggc aatcctggaa
     1681 cttcgacgag gaatatcttt ttacagaata taagaagaac aggttccggg agtcgtttca
     1741 ttttaatgat gaatcgttta aactccaatt taaggaatat attccaaatg cagccgaagc
     1801 agtggtagag gctaaaaacg gcataccgat gatgactttt gtcacggtag accgtggcag
     1861 ccgccggacc agcctgctca aagccggcga agtcaaaacc gtcagagggc ttgcgcttag
     1921 ctttaatgct cccaccgacg attcaacagc catccggatc aattactcac ctgaaacagg
     1981 gctgactgtg cgtaccccct tcccgtcgac cgtgatgaat atgatggccg gtactaccga
     2041 cacccttcgg aaagattcta cctacaccct tttccagatg caattgtacg atttcatggg
     2101 catgcagttc gttgtccggc agttttatcc ctcgggaaaa acccagctgg tctcttctcc
     2161 ccatgaatcg agcggcgtgg cagctattgt catgaacctt tcctataacg gtaaaacaga
     2221 agaagtcggc atgctgggcg gaaagggaat ggtcggccag gcggtgacaa catcgatcga
     2281 cggaaccgaa ttctcattgt cgtacggctc caaatacatc ggcctgcctt tctatatcaa
     2341 gctcagggat ttcatcctgg aaagataccc gggatcgaac agcccttcgt cgtttgccag
     2401 cgaagtcacc gtcatcgacc cttcggaaaa cctggaaatg ccctaccgta tcttcatgaa
     2461 caatattctg aattacggcg ggtatcgttt cttccagtca tcctacgata aagatgaaaa
     2521 agggacagtc ttatccgtta acagcgatta ctggggtacg ttgtttactt atatcggtta
     2581 tgcaatcctt gctgccgggc tgctgtacaa tttcttcagc cggaacagcc gtttccgcaa
     2641 gctcgccagg atgacttcag aactcaaaaa gaagagcgca atggtggtga tcgcgatact
     2701 gatcatggtt gggttttcag cggaaaatgc tggcgcacag cacgacggca acttcaatcc
     2761 cttgaatatc agtaaagaac acgccaagaa gttcggagag ctgatcgtac aggaccagga
     2821 cgggagaatg aagcctgtca atacgctatc ttccgaatta ctaaggaaga tttaccggaa
     2881 agatacttac aaagggctga acagcgaaca ggtcttcctt ggcattatct ccaatccgga
     2941 gtactggcag ggcatcccgc tcattaaagt atctgatgac gaacttaaaa cagctattgg
     3001 tgcggatggc aaatatgcat cttttaacga ttttcttgac aacaggcaag gcttctacaa
     3061 gcttgggcaa catatagaag cggctaacaa taaaaaacct gttgagcgaa ccagttttga
     3121 taaagatgtg atcaaggtcg acgagcgggt gacgcaaggc caggcggtgg gtattatagc
     3181 ctttg
//
LOCUS       DAOQXS010000369         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32203_length_3179_cov_3.747439, whole genome shotgun sequence.
ACCESSION   DAOQXS010000369 DAOQXS010000000
VERSION     DAOQXS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32203_length_3179_cov_3.747439"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKM34_09825"
     CDS             <1..850
                     /locus_tag="PKM34_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83930.1"
                     /translation="GNFPGAPNTLGSIVLFDSLFNYISADSLPYGLHFQHTAQWLNDS
                     VYLVTGEKFLDGGGKRDIGICKMTEDDQFIAGNHFGKGGDTITKAGVSSNMDFNDSGN
                     IYFGGITNVNIAYGIYQHDSWLILENIDSNLNQNWQRFYGGDAGYYLWALKATADGGC
                     VMMATRYDPEVQDGETDVTIIKVDSNGLLTSTGNGPAIPAQQLAIHPNPASRKITIAY
                     PDIFKFQQKEILIYNIYGNKIYQHDITADISRSYADISSYPNGLYIVVLSVEGKKIAS
                     GKVIKY"
     gene            1242..2663
                     /locus_tag="PKM34_09830"
     CDS             1242..2663
                     /locus_tag="PKM34_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ83931.1"
                     /translation="MKKSLLIILCSLFTLHGSLAQNERMQWFKDAKLGIFIHWGIYAV
                     NGIDESWSFYNELLPYEDYLKQLDGFGAENYDPQAWAGLIRESGARYAVLTSKHHDGV
                     ALWDTDAGDLSVVKRTPAGKDLIGPFCSALRAEGLKVGLYYSLLDWSHPDYPNFTRNR
                     KRYERDSIPSRWDNFDRFNRAQITEITTRYNPDLVWFDGDWEQSAEVWKAAEIREMLL
                     NNNTNVIINSRLQGYGDYATPEQGIPVSRPKDEYWELCMTMNDSWGFQPNDTNYKPVN
                     LIIRIFADVIGMGGNLLLDIGPKADGTIPEEQVAILKELGRWTSKHSEAIYGTLAGLP
                     KDCYYGSSTLSADSTVLYLFIPSNSKSQVSNSKSQVPNSKQNPNLKSENPIENPQILK
                     SPNPQISQSSYSPAILLKGITNKINSIRVVGNGTKLEQTTWLKPWWSSNPGLIGISIP
                     AEAMDPDMTVIAVEFEGKLKVKF"
     gene            complement(2758..>3179)
                     /locus_tag="PKM34_09835"
     CDS             complement(2758..>3179)
                     /locus_tag="PKM34_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HNQ83932.1"
                     /translation="LIASALGARVYPNKTKEIGWFPVTAIKPPQISNFQFPASFTTLH
                     WHGETFDLPERAALLASSEATKNQAFQYGKNVLAIQFHPEANMQTLQDFCFHFKKELV
                     PGRFIQSEEEIVKYADEYLSSMDDLIDRLMLYLVGDE"
BASE COUNT          898 a          763 c          752 g          766 t
ORIGIN      
        1 tggaaatttt ccgggagctc caaataccct tggatccatt gttttattcg attccctttt
       61 caattacata tctgctgatt ccctgcctta cgggcttcat ttccagcata cggcgcaatg
      121 gttaaatgat tcggtgtatc tggttaccgg agagaaattt ttagacggag gtggaaaaag
      181 agatattggc atttgcaaaa tgacggaaga tgatcaattt atagccggca atcattttgg
      241 gaaagggggt gatactatta caaaagcagg ggtatcttct aatatggatt ttaatgactc
      301 agggaacata tactttgggg gcataaccaa tgttaatatc gcctatggca tttatcagca
      361 tgattcgtgg ctcatccttg aaaatatcga ttcaaacctc aatcagaact ggcaaagatt
      421 ctacggtggg gatgccggct attatctttg ggcacttaag gccacggccg acggcggatg
      481 cgtaatgatg gccacccgtt acgatcccga agtacaagat ggcgaaaccg acgtcaccat
      541 tatcaaagtc gattccaatg gattgcttac atctaccggg aatggaccgg ctatccccgc
      601 ccaacagctt gccatccacc cgaatcccgc atccagaaaa atcacaatcg cataccctga
      661 tatctttaag tttcaacaaa aagaaatcct gatttataac atttacggaa acaagatcta
      721 tcagcatgac atcactgccg atatatcaag atcatatgcc gatatcagta gttatcccaa
      781 cggattgtat atcgttgtac tcagcgttga aggaaagaag attgcatccg ggaaagtgat
      841 aaaatattga tgaaggccgg cagcatttcc tctaagtgcc tcttagtgaa ctcagtgtct
      901 aagttgttat caaaataaga agcaaccgct taataaccat tagcgacaca aaccttccgg
      961 taaaaattca acttatccac ctcaaatcgg agcagataca ctccggcacc gaatcgggcg
     1021 gacaaatggt aagcacaccg ttttcatagg aaatgcagcc cggaggagga taaacctggc
     1081 aaagggcaaa acctgaaatg acgattaatg cgcaacaaat taagaatgat ttcattgtaa
     1141 aaatgttagc ctggtatctg cctgctaaag atacgaattc tcaggtgata tttctgaatc
     1201 atccggaatt caatataaat ttgattgaaa tataaaccat tatgaaaaaa tctttactaa
     1261 taatactctg ttcactcttc acccttcacg gttcacttgc tcagaatgag cggatgcaat
     1321 ggttcaaaga tgccaaactc ggaattttca tccactgggg gatctatgcc gtgaatggca
     1381 tcgacgagtc gtggtcgttt tacaacgaat tacttcccta tgaagattac ttgaagcaac
     1441 ttgacggctt tggcgccgaa aattacgatc cgcaggcctg ggccggcctg atccgggaaa
     1501 gcggcgcccg ctatgccgtt ctgacttcga aacaccacga cggcgttgcg ctgtgggaca
     1561 ccgatgccgg cgacctgtcg gtggtaaaga gaacgccggc gggaaaagat ctcatcggtc
     1621 cgttctgcag cgcactgagg gcggaagggc tgaaagtcgg gctgtactat tcgctgctcg
     1681 actggtcgca tcccgattac ccgaacttta cccgcaaccg gaaacgctac gagagggact
     1741 ccatcccctc gcgctgggat aatttcgacc ggttcaaccg ggcccagatc accgagatca
     1801 caacgcgcta caatcccgac ctggtgtggt tcgacggcga ctgggaacaa agcgcggagg
     1861 tgtggaaagc ggcggagatc agggagatgc tgctcaataa caataccaat gtcattatca
     1921 atagcaggtt gcagggatat ggagattatg ccacgccgga gcaggggatc ccggtttcaa
     1981 ggccgaaaga cgaatactgg gaactgtgca tgaccatgaa cgactcctgg ggtttccaac
     2041 ccaacgatac gaactacaag ccggtaaacc tcatcatccg catctttgcc gacgtgatcg
     2101 gcatgggcgg caacctgctg ctcgacatcg gcccgaaagc cgacggcacg ataccggaag
     2161 agcaggtcgc tatcctgaaa gaactgggcc gttggacttc gaaacacagc gaagcgatct
     2221 acggcacgct ggcggggctg cccaaggatt gctactatgg cagttcgaca ttatctgctg
     2281 acagcactgt tttgtatctc tttattccgt caaattccaa atcccaggta tcaaattcca
     2341 aatcccaagt accaaattcc aaacaaaatc caaatctcaa atccgaaaat ccaatcgaaa
     2401 atccccaaat cctcaaatct ccaaatcccc aaatctccca atcatcttac agccctgcca
     2461 tcctgctgaa gggaattaca aacaagatca acagcatccg ggtcgtcggc aacggcacaa
     2521 agctggagca gactacctgg ctgaagccgt ggtggagcag caatcccggg ctgatcggga
     2581 tttccattcc cgcagaggcg atggacccgg atatgacagt gatcgcggta gaattcgaag
     2641 ggaaacttaa ggtgaagttc tgataattat ttaccacata ggcacataga gcacatagga
     2701 tcacatagga tctaatttgt tttctatgtg ttctatgtgc ctatgtggtg aaaataatca
     2761 ttcatctccg accagataaa gcatcagtct atcaataaga tcatccattg aagaaaggta
     2821 ttcatccgca tatttgacaa tttcttcctc actctgaatg aaccttcccg gcaccagctc
     2881 cttcttgaaa tgaaaacaga aatcctgtag cgtctgcatg ttagcttcgg ggtggaactg
     2941 gatggccagg acattttttc cgtactgaaa ggcctggttt tttgtggctt cgctgctggc
     3001 caggagcgct gccctttcgg gcaggtcgaa cgtttcgccg tgccagtgca aggtcgtgaa
     3061 tgaagcagga aattggaaat tggaaatttg gggtggcttt attgccgtca cagggaacca
     3121 gccgatctct ttggttttgt tgggatagac ccgggcgccg agggcgctgg caatgagct
//
LOCUS       DAOQXS010000370         3160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32516_length_3160_cov_3.011916, whole genome shotgun sequence.
ACCESSION   DAOQXS010000370 DAOQXS010000000
VERSION     DAOQXS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32516_length_3160_cov_3.011916"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="nuoK"
                     /locus_tag="PKM34_09840"
     CDS             complement(<1..264)
                     /gene="nuoK"
                     /locus_tag="PKM34_09840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNQ83933.1"
                     /translation="MIEGIPIVHFLIVTTIMFFVGVYGFLVRRNLITILMSLELMLNS
                     VAINFVVFNKYLYPGHLQGHFFSLFIIAVAAAEAAVAIAIIINV"
     gene            complement(283..792)
                     /locus_tag="PKM34_09845"
     CDS             complement(283..792)
                     /locus_tag="PKM34_09845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNQ83934.1"
                     /translation="MNILMFYLFASVIVIFSILTVTSRRILRSAVFLLFVLIATSAFY
                     FMLRYTFLAAAQLALYAGGIVVLIIFSILLTSHISERFPQITFKKAFFSALVAGAGAV
                     LSIVTLLQFDFDQQYDGGQTAELPVRDIGKSLMDFGEEGFVLPFEVISILLLAAMIGA
                     IVIAKKKKD"
     gene            complement(794..1276)
                     /locus_tag="PKM34_09850"
     CDS             complement(794..1276)
                     /locus_tag="PKM34_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ83935.1"
                     /translation="MKGIVNYIREIIQGVITLIKGMAVTGKYFVTSPKTIVTQKYPEN
                     RKELEMFDRFKGEVIMPHNENNEHRCTGCGICEMNCPNGSIEIITKTEEIDGKKKRVI
                     DKHIYHLSMCTMCALCVKTCPSNALAFGQTFEHAVYDRKQLTKVLNKPGSVLTKEIKE
                     "
     gene            complement(1313..2323)
                     /gene="nuoH"
                     /locus_tag="PKM34_09855"
                     /pseudo
     CDS             complement(1313..2323)
                     /gene="nuoH"
                     /locus_tag="PKM34_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
     gene            complement(2420..>3160)
                     /locus_tag="PKM34_09860"
     CDS             complement(2420..>3160)
                     /locus_tag="PKM34_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNQ83936.1"
                     /translation="DLGALTTFFYGLRDREKILDIFEETCGGRLLLSYNTPGGVMYDI
                     HPNFQKRVKEFIKYFKERLPEYDQLLTGNVIFQERTKGIGRLTREEAVNYGVTGPSGR
                     GSGFSCDIRKKFPYSAYPYVDFKEILGVDGDALSRYKVRMEEMWESMHIIEQLIDNIP
                     EGPYTAKMKAVLKLPAGEYFQRVETARGELGVYVISDGNKTPYRMKFRSPNMCNLSAL
                     DHISRGAKIADLVAIGASFDFVIPDIDR"
BASE COUNT          809 a          793 c          814 g          744 t
ORIGIN      
        1 aacattgatg atgatggcga tggccactgc cgcttccgct gccgccaccg cgatgatgaa
       61 cagggaaaag aaatgtccct gcaagtgccc cgggtagagg tatttattga aaacgacaaa
      121 attgatggcg accgagttca gcatcagttc cagcgacatc aggatggtga ttaggttgcg
      181 gcgcaccagg aagccataga ctccgacaaa gaacatgatc gtagtgacga tcaggaaatg
      241 gacgattgga atgccttcta tcatgatgat tggttttaca ggttaatctt ttttcttttt
      301 tgcgatgacg attgctccta tcattgcggc cagaagcagg atggaaatga cctcaaacgg
      361 caggacaaat ccttcttccc cgaaatccat caggctcttt ccgatatccc tgacaggtaa
      421 ttcggcggtc tggccgccgt cgtactgctg atcgaagtcg aactgaagca aggtgactat
      481 gctcagaacc gccccggcac cggctaccag ggctgagaaa aaggcctttt taaaggtgat
      541 ttgcggaaac cgctcgctga tatggctcgt cagaagaatg gaaaagatga tcagaacaac
      601 gatgccgccc gcatacagtg ccagctgagc cgctgccagg aaagtgtacc gcagcatgaa
      661 ataaaatgcc gaggtggcga tcagcacaaa caaaaggaaa acggctgaac ggagaatcct
      721 ccggctcgta acggtcagga tcgaaaaaat gacgatcacg ctcgcgaaaa gatagaacat
      781 caatatattc atactattct ttgatttctt tggttaatac tgagccgggt ttgttcagca
      841 ctttcgtcag ttgcttccgg tcataaacgg catgctcgaa tgtctgcccg aaggcaaggg
      901 cattggaagg gcatgtttta acacataagg cgcacatggt gcacatggag agatgataaa
      961 tgtgcttgtc gatcacccgc ttcttcttac cgtcgatttc ctcggtttta gtgatgatct
     1021 cgatagagcc gttggggcag ttcatttcgc agattccgca accggtgcaa cggtgctcgt
     1081 tgttttcgtt gtgtggcatg atgacttccc ctttgaaccg gtcgaacatt tcgagttctt
     1141 tccggttctc agggtatttc tgcgtgacga tggtcttggg gctggtgaca aaatatttgc
     1201 cggtcaccgc catgcctttg atcagggtga tcaccccctg gattatctct cttatataat
     1261 tgacaatgcc tttcatatgt tcatttttac gttgaaagat cagctctacc ggttagaaat
     1321 gccatttcat catggtcagg aaagcgatca ggaccgtatt caccaggttg atcggcagca
     1381 aatatttcca ttcgagcgtc agaagctggt cgatcctcaa ccgggggaag gtccatttga
     1441 accacatcat taaaaagatc acgaaagcgg tctttcccat ataccatact accggcggga
     1501 tatagtccat tacccggtta aaggcgtcaa aaccccagaa gtgcaggggc atccagcctc
     1561 ccaggaaaac cgtggcggcg atggaagcga caatgaacat gttcatgtat tcggccagga
     1621 agaagaaggc gaatttgatc ccggagtatt cggtgtggta gccggccgtc agttccgatt
     1681 cggcttcggc caggtcaaag ggtccgcggt tagtttcggc agtggctgcg atcaggaaga
     1741 tcacaaagga gatgaccgcc gggatgtgcc ctttgaagat ccaccaggca tcggcctggc
     1801 tctctacgat ctctgagaat tgcagcgtgc ctgaaaagat aacgaccgaa agcagcgcca
     1861 ggccgaccga cagctcgtag ctgacaatct gcgcaccgct ccggatggcc ccgatcagcg
     1921 aatacttgtt gttgctcgac catcccgcaa ggagaatacc cacaacaccc atggatgata
     1981 ccgccatcac ataaaaaacg cctatgttaa aatcgatggc atggagccct tttgcaaatg
     2041 gaattgcagc gatggccatg aaggaggcga tgatgacgat aaacggagcc aggttgaaca
     2101 ggaacttgtc ggcctccttg atctttacca gctctttcat caacagcttg ataaagtcgg
     2161 cgatggtctg cagcaatccc caggggccga cccggttggg tcccagccgg ttttgcatga
     2221 aggcacagac acgcctctcg gcatagacca ggatcaggcc ggtaacggca taaaaaatca
     2281 ggaataccag gccgatgatg accatctccc aggcaatggc ccagaatggg gtgaacgttt
     2341 cctggagcca ggcttcgatg ccggcggtca gggaagtgaa atcgtataac cagtgatctt
     2401 tcataaggta atgcgaatat tagcggtcaa tatctggaat aacaaaatca aaggaagccc
     2461 cgatggccac caggtcggca atctttgcgc cgcgcgagat gtggtcgagg gccgaaaggt
     2521 tgcacatatt cggcgagcgg aatttcatcc ggtaaggggt cttgtttcca tcgctgatga
     2581 cgtacacgcc aagctctcca cgggctgttt ccacccgctg gaagtactcg ccggcaggca
     2641 gtttcagcac cgccttcatc ttggcggtat aaggcccttc cgggatatta tcgataagct
     2701 gctcgatgat gtgcatcgat tcccacatct cctccatcct caccttgtag cggctcagcg
     2761 catctccatc cactccgagg atctctttga aatcaacata aggataagca ctgtaaggga
     2821 attttttacg gatatcgcat gaaaagcccg aaccgcgacc ggaaggccct gttacaccat
     2881 aatttacagc ttcttcccgc gtcagtctgc cgattccttt ggtccgttcc tggaaaatga
     2941 cattgccggt cagcaactgg tcgtattcag gaagcctttc tttgaaatat ttgatgaact
     3001 ctttgactct tttctggaaa ttcggatgga tatcgtacat cacgccaccc ggagtgttgt
     3061 agctgagcag caaacgcccg ccgcaggtct cttcgaaaat gtccaggatc ttttcgcggt
     3121 cgcgtaaacc atagaaaaaa gtggtcagcg ccccgaggtc
//
LOCUS       DAOQXS010000371         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32721_length_3147_cov_3.074386, whole genome shotgun sequence.
ACCESSION   DAOQXS010000371 DAOQXS010000000
VERSION     DAOQXS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32721_length_3147_cov_3.074386"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKM34_09865"
     CDS             <1..198
                     /locus_tag="PKM34_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83937.1"
                     /translation="AKERMIVLSAFSLFFISHWIILSFAANKMKENDLAVLSPIYEVL
                     LLIISPLLYISNIVHKQERWK"
     gene            189..809
                     /locus_tag="PKM34_09870"
     CDS             189..809
                     /locus_tag="PKM34_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ83938.1"
                     /translation="MEISQRMTGKAIRDFDLVKAALEKGDQNAYAELMKNYRDALYFM
                     MLKMTNNPHDAEDLTIEAFGKAFRNLRQYTPDYAFSTWLFKIASNNCIDFIRKKKLNE
                     NISFNLNDLPDSGDGLADILPTTSRNPEEDIIRKQKIETLRLVIDKLKPNYRRLIELR
                     YYEEKSYEEISEALDLPIGMVKAQLFRAREFLYHFLSNRHDKLYEE"
     gene            893..1768
                     /locus_tag="PKM34_09875"
     CDS             893..1768
                     /locus_tag="PKM34_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83939.1"
                     /translation="MKRLKILIIILMGALMITSCGKKKEQQILDLQAKNAELLAESNR
                     KDSLMNELFTSINDIERNLADVTAREKLITDAPVSEQRMNEDVRTKIMDEIILINNIM
                     EENKKQIAALKDQLKKSGAKVAALQETINLLTQRLEEKEMEIADLKGQLVRQNFRIEE
                     LNATVDTLQEEKASQSRIISQQDALIGEMNTIWYVSGPKKELLEKGIIEKAGGMLSGK
                     MKMSDLINPGYFVTADMRKISVIPFEGDKAEMVTVHPEGSYKFLMEGDTFKGIEILNP
                     GEFWKSSRFLVIMTK"
     gene            1771..>3147
                     /locus_tag="PKM34_09880"
     CDS             1771..>3147
                     /locus_tag="PKM34_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNQ83940.1"
                     /translation="MKLIDFQKAIATGIGDVLPEPKPYDRSVSHAPRRKDILTPEEKR
                     LALRNALRYFKPEHHPVLASEFATELERFGRIYMYRFRPDYPMFARPIGEYPHRSLQA
                     AAIMLMIQNNLDPAVAQHPHELITYGGNGAVFQNWAQYLLTMRYLSEMTNEQTLVMYS
                     GHPLGLFPSHPDAPRVVVTNGMVIPNYSSQDDYERFNALGVSQYGQMTAGSYMYIGPQ
                     GIVHGTTITILNAGRRLDPGNGSLAGKVFVTSGLGGMSGAQAKAAVIAGAVGVVAEVN
                     PAAVSKRHEQGWVDEVFTDLDLLLARIQKARTLREAVSIAYQGNIVDLWEKMAEQQFD
                     VELGSDQTSLHNPWAGGYYPAGITFEESNRLMAEDPARFRELVKGSLIRHVKAINTLA
                     ARGMYFWDYGNAFLLESGRAGADVFRPDGSYRYQSYVQDIMGPICFDYGFGPFRWVCT
                     SGKAEDLRT"
BASE COUNT          896 a          693 c          789 g          769 t
ORIGIN      
        1 gcaaaagaga ggatgattgt tctgtccgct ttttcgctgt tttttatcag ccactggatc
       61 atcctctctt ttgctgctaa taaaatgaaa gagaacgatc tggcggtatt atctcccatc
      121 tacgaggtgc tgctattgat tatcagtcct ttactttata tttccaatat cgttcacaaa
      181 caggagagat ggaaataagc cagcggatga ccggtaaagc gatcagggat tttgacctgg
      241 ttaaagctgc cctggaaaag ggagaccaga acgcctatgc cgaactgatg aagaattacc
      301 gtgacgcttt gtatttcatg atgctgaaaa tgaccaataa tccgcatgat gcggaagacc
      361 ttactattga ggcctttgga aaagctttca ggaatctgcg tcagtatacc ccggattatg
      421 ctttcagtac atggctcttc aagatcgcct caaataattg catcgatttt atccgcaaga
      481 aaaaactgaa tgaaaatatc tcattcaatc tgaatgactt accggattcc ggtgacggac
      541 tggccgatat tcttcctacg acatccagga atccggaaga agatatcatc cggaaacaaa
      601 aaattgaaac cctgcgcctt gtgatcgata aactcaaacc gaactaccgc aggctgatcg
      661 aattaaggta ttacgaggag aaatcgtacg aggagatatc ggaagcactt gatctcccta
      721 ttggtatggt taaagctcag ctcttcaggg ccagggaatt tctttatcac tttctgagta
      781 accgtcacga caaactatac gaggagtgat cccggaaatc aatatttttt attcggataa
      841 tatatttgta ttaatttagt tatttcgaat gagttaatta aaacttcagc acatgaaaag
      901 actaaagatt ttgatcatta ttctgatggg agcattgatg atcacttcct gtggtaagaa
      961 aaaagaacag cagatcctgg atctccaggc aaaaaatgcc gaattactgg cagaatccaa
     1021 ccggaaagac tcactgatga acgaattatt tacttcgatt aatgacattg aaaggaacct
     1081 ggcggatgtc acggcccgtg aaaagctgat caccgatgca cccgtatcgg agcagcggat
     1141 gaacgaagat gtacgcacaa aaattatgga tgagattatc ctcatcaata acataatgga
     1201 agaaaataaa aagcagattg ccgctttgaa agaccagctg aagaaatcag gcgccaaagt
     1261 ggctgctctt caggaaacca tcaatctttt aacccagcgc ctcgaagaaa aggaaatgga
     1321 gattgctgac ctgaaaggtc aacttgtacg tcaaaatttc agaattgaag aattgaatgc
     1381 taccgttgat acccttcagg aggaaaaagc ctcgcagtcg aggatcatca gtcagcagga
     1441 cgccctcatc ggggagatga acacgatctg gtacgtttca ggtccgaaaa aagaacttct
     1501 ggaaaaaggg ataatcgaga aagccggggg aatgttgagc ggaaaaatga agatgagcga
     1561 cctgatcaac ccgggatatt tcgttaccgc cgatatgcgg aagatttcgg ttataccttt
     1621 tgaaggtgac aaagcggaaa tggtaacggt tcatcccgaa ggttcataca aatttttgat
     1681 ggaaggcgat acatttaaag ggattgagat tctgaatccg ggggaattct ggaagtcatc
     1741 ccgctttctg gtgattatga ctaaatgatg atgaaattga ttgattttca aaaagctata
     1801 gcaactggta tcggagatgt cctgcccgaa ccgaagcctt atgatagaag tgtaagccac
     1861 gcacccagaa gaaaagacat cctgacgccg gaagagaaaa ggctggctct gcgcaatgct
     1921 cttcgctatt ttaaaccgga acatcatccg gtgctggcat ccgaatttgc gacagaactt
     1981 gagagattcg gccgcattta catgtaccgg ttcaggccgg attacccgat gtttgcacga
     2041 cccatcggcg aatatcccca tcgctctttg caggccgccg ccattatgct gatgatccag
     2101 aataacctcg atccggctgt tgcgcagcat ccgcatgaac tgatcaccta tgggggtaac
     2161 ggggctgtat ttcaaaactg ggcgcagtac ctgctgacaa tgagatattt gtcggaaatg
     2221 accaatgaac agaccttggt aatgtattcg ggtcatccgc tcggcttatt tccgtctcac
     2281 cccgatgcac ccagggtggt ggttaccaat ggaatggtca ttccgaatta ttcttcgcag
     2341 gacgattacg aaagatttaa tgcgctcggc gtttcgcaat atgggcagat gactgccggt
     2401 tcgtacatgt atatcggccc gcaggggatt gtgcacggta caacgattac catcctcaat
     2461 gcaggacgca ggcttgatcc gggaaacgga agcctggcag ggaaggtttt tgttacttcc
     2521 ggcctgggcg gtatgtcggg tgcgcaggct aaagcagcgg taattgccgg cgcagtggga
     2581 gtcgttgcag aagttaaccc cgcggcagtc agtaaaaggc atgaacaggg atgggtagac
     2641 gaagtgttca ctgatcttga cctgttactc gccaggattc aaaaagccag aaccctccgg
     2701 gaagccgtat ccattgctta ccagggaaat attgtcgacc tgtgggaaaa gatggcggaa
     2761 cagcaatttg acgtggaact gggctccgat cagacctcgc tgcataaccc ctgggcaggc
     2821 gggtattacc cggccgggat cacttttgaa gaatctaacc gcctgatggc ggaagatccg
     2881 gcccgtttca gggaattggt gaaaggatcg ctcatccgcc atgtgaaagc tattaacacc
     2941 cttgcagcca ggggaatgta cttctgggat tacggcaatg ccttcctgct ggagtcggga
     3001 agggccgggg cggatgtctt ccgccccgac ggtagttacc ggtaccagtc atacgttcag
     3061 gatattatgg gcccgatctg ctttgactac ggtttcggcc cgttccgctg ggtctgtact
     3121 tccggcaaag cggaagacct cagaacg
//
LOCUS       DAOQXS010000372         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32802_length_3141_cov_3.545690, whole genome shotgun sequence.
ACCESSION   DAOQXS010000372 DAOQXS010000000
VERSION     DAOQXS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32802_length_3141_cov_3.545690"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKM34_09885"
     CDS             <1..1261
                     /locus_tag="PKM34_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83941.1"
                     /translation="ERHFFPPSKYRPSGLITSVLLLSIAGLMPWILIPANDQDEKKPV
                     LPEGNFKRGYLIETITPVEIKPDGNGDYRISARSFTVRPTVYEEKEAIPEAGASEQFI
                     ASINDPADDPAIRPILIANRQHIADLAEPAIPNTAVKEGLLKDASIAFLESHPVGLIQ
                     YSRYIEEMKISAELRKTSLTPYHEKSYVRKGEFSAGLNFNPSIVFYDPNPYNKMLGGD
                     ATISYKMFSFSIMTGIGYSRMEDVSTYKVDYMTNDSVGYFIRVIGFIPDPRNPGEITY
                     ITRQEAIYDSVPHYTIEDKTNYYSYIDIPFSVGYTLLQTHRISLTIDAGIKFSLLVDR
                     DEPDIDFRISDAELINVERQVPARLNTNWRFTAGVDFGYILSDRFSLHLEPVFEQYIS
                     PVYAKQPGYLPRKPYLTGVKAGIRYNF"
     gene            1291..2583
                     /locus_tag="PKM34_09890"
     CDS             1291..2583
                     /locus_tag="PKM34_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ83942.1"
                     /translation="MILSAMMVLPAMEIVGQISSLPDKTQTDQRDHALSTDDACQASF
                     KATPDSLSSFPYLYHFNDLSTGIGLVSSWHWDFGDGTTSTERNPSHQYEAPGTYRVCL
                     VISGQNDTVNCDDEFCFDVATLNYYSLGGLVYAGDYPLNNPDNQGDVGIASLYRLVND
                     QIVFVEDQTFQEYGYFWFGYLFPGDYMVKVGLTPESPSYNEYFTTYYGDEVSWTKADI
                     LSVSSVSQFDAEVHLAPVQELSSGAGTIKGYVKFEQGNQYNMPPIAQTNVILADKNHV
                     PLLFTHPNAAGYFEFSSLPVDSYYLSADATGKPSSTVLVTLTQAVPVVEGINLTVFGS
                     NISFIPDETSGNQLMIKLFPNPAGDNLYISMYSVYSDPIEVTICDITGKIQHRSSVVF
                     EPGTNTWVIPVNNLPAGIYLLRIQQPGILQPITARFIK"
     gene            2682..>3141
                     /locus_tag="PKM34_09895"
     CDS             2682..>3141
                     /locus_tag="PKM34_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83943.1"
                     /translation="MKKLIILILTGILVTGFSQGQGGWREYEMEVKIVFGRLEDASKL
                     GALNLNGDIYSAKGYAILYVTPDELDLINSKGFKTEILKQDLNLYYHDFWTSRDQYHT
                     YDEIIDVIDSLVETFPQIAKKYTYGTSLGGRQLCALKISDNVYADEPEPEV"
BASE COUNT          816 a          766 c          740 g          819 t
ORIGIN      
        1 cgaaaggcat tttttcccgc cttccaagta caggccatcc gggttgatca cctcagtcct
       61 gctcttatct attgccggac ttatgccgtg gatactgatc ccggctaatg accaggatga
      121 gaagaagccg gttttgccag aaggcaattt caaaagagga tatctgatcg agaccattac
      181 cccggttgag atcaaacccg atggaaacgg cgattaccgg atatcggcca gaagttttac
      241 tgtcaggccg actgtttatg aagagaaaga ggccattcct gaggccggtg cttccgaaca
      301 gttcattgct tcaatcaatg atcccgctga cgatcctgcc attcggccca tcctcatagc
      361 caaccggcag catattgccg atctggccga accggctata cccaatacag ccgttaagga
      421 agggttgctg aaagatgcaa gcattgcttt cctggagtcg catcctgtcg gactgataca
      481 gtacagccgt tatattgaag aaatgaagat cagtgccgaa cttcgtaaaa cttcgctgac
      541 tccctaccat gagaagagtt atgtcagaaa aggtgaattc tcggccggtc ttaatttcaa
      601 cccttccatc gttttctatg atccgaaccc gtataataaa atgctcggcg gggatgcaac
      661 cattagctat aagatgttct ccttcagcat tatgaccggt atcggttatt cgcggatgga
      721 agatgtgagc acttataagg tggattatat gacaaacgac agcgtggggt attttatccg
      781 cgtcatcggt tttatccccg acccgagaaa tcccggagaa atcacctata tcacccgcca
      841 ggaagcgatt tatgactccg tgcctcatta cacaatagaa gataaaacca actactattc
      901 ctatatcgac attccgttct ctgtgggtta cacattatta cagacccaca ggatttcgct
      961 gaccatcgat gccgggatca agttcagcct gctggttgac agggatgaac ccgatatcga
     1021 tttccggatt tcggacgccg aactgatcaa tgtggagcgg caggtgcccg cccggctgaa
     1081 taccaactgg aggtttacag ccggtgttga tttcgggtat atcctatcag accggttcag
     1141 cctgcatctc gagccggtat tcgaacaata tatcagtcct gtttacgcaa aacaaccggg
     1201 ttatcttccg agaaagcctt atttaacggg agtaaaagca ggtataaggt ataactttta
     1261 agcgtcgatc tcatgaaaac cttacacgca atgatattat ccgcaatgat ggtcctgcct
     1321 gcaatggaga ttgtgggaca gatttcatcc ttgcccgaca aaacacaaac tgatcagagg
     1381 gaccatgcgt tgtcgacgga tgatgcctgc caggcatcgt tcaaagccac gcccgattcg
     1441 ctgtcaagtt ttccatacct gtaccatttt aatgatcttt cgacaggtat cgggctggtg
     1501 agttcctggc actgggactt cggagacggg acgaccagca cggaaagaaa tccgtcgcac
     1561 caatacgaag cccccgggac ataccgggtt tgcctggtca tttcgggtca gaatgatacc
     1621 gttaactgtg atgacgaatt ctgttttgat gttgccacgc tcaactatta cagcctggga
     1681 ggcctggtgt atgccggtga ttatccgctg aataacccgg ataaccaggg tgatgtcggt
     1741 atcgcttcgt tgtaccggct ggtcaacgac cagatcgttt ttgtggaaga ccagactttc
     1801 caggaatacg gatatttctg gttcggttat ctattccccg gcgattatat ggtgaaagtg
     1861 ggattgacgc ctgaatcgcc gagttataac gaatatttca ccacttatta cggggatgaa
     1921 gtatcctgga cgaaagcaga tatcctctcc gtttcatccg tcagccagtt tgatgcagag
     1981 gtacaccttg ctcctgtaca ggaactttca tccggtgcag gcactatcaa aggatacgtt
     2041 aaattcgaac agggcaacca gtacaatatg ccgcccattg cccagaccaa tgttatcctg
     2101 gctgataaaa accacgttcc gttgttgttt acccatccca acgcggccgg ttacttcgaa
     2161 ttttcctcat tgcctgtcga cagctattat ctgagtgccg acgccaccgg aaaaccttcg
     2221 tcgaccgttc ttgtgaccct tactcaagcg gttccggtgg tggaagggat caacctgacg
     2281 gtgttcggtt caaatatcag ttttatcccc gatgaaacaa gtggtaacca gcttatgata
     2341 aagctatttc ccaacccagc cggtgataac ctttacatca gcatgtattc ggtttactct
     2401 gatccaatag aggttaccat ttgcgatatc acgggaaaga tccagcaccg gtcatcagtg
     2461 gtttttgaac cgggaacaaa cacctgggtc attcctgtga acaacctccc ggccggaatt
     2521 tacctgctcc gcattcagca gccgggtata ttacagccta ttacggcacg ctttataaaa
     2581 taataggccc cccgggggaa tttctttcgt acttttgagc gtggatcaac cccggcaggt
     2641 tcatctttat gaactgctga ccggcgaagc attattttca aatgaaaaaa ctcatcatac
     2701 ttatacttac cggtatattg gttaccgggt tttcgcaagg gcaggggggc tggcgcgaat
     2761 atgaaatgga ggtaaagatc gttttcggcc ggttggaaga tgccagtaaa ttgggtgcac
     2821 tgaatcttaa cggcgatata tactctgcta aaggctacgc tattctgtat gtaacgcctg
     2881 atgaactgga ccttattaat agcaaaggtt tcaaaacgga aatcctgaaa caggacctga
     2941 acctatatta ccatgacttc tggacttccc gggaccagta tcacacatat gatgagatca
     3001 ttgatgtcat tgattccctg gtggaaactt ttccgcagat cgctaaaaaa tacacttacg
     3061 gcacttcttt aggcggcagg cagttgtgtg ccctgaaaat ttcagacaat gtttatgccg
     3121 atgagccgga gccggaggtc a
//
LOCUS       DAOQXS010000373         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32953_length_3132_cov_3.181995, whole genome shotgun sequence.
ACCESSION   DAOQXS010000373 DAOQXS010000000
VERSION     DAOQXS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32953_length_3132_cov_3.181995"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1261)
                     /locus_tag="PKM34_09900"
     CDS             complement(128..1261)
                     /locus_tag="PKM34_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNQ83944.1"
                     /translation="MKHPVIIIDDHIPFIKGVLEPFARVTYAPGGVISKDIASKADGL
                     IIRTRTRCNAELLDGIPVKFIASATIGTDHIDIQYCNNRSIEWYHAPGCNSGSVMQYM
                     ASAFSRLSTKYHISLAGKTIGIIGAGNVGKKIALLAEILGMSPLLNDPPRARNEGQQG
                     FVSLQKIKEECDLITFHVPLNLGGDDRTFHLANDAFFNSLAKKPLIINTSRGAVVDGS
                     ALKVAHQKGMIRGFVADVWEDEPHIDRELLAIADIATPHIAGYSVEGKANGTAAVVRA
                     ASRYFGFGIDEWYPENLPGPGEPVIEIKSAGKSEERIIDEAILGTYDVLKDDRTLRND
                     PLQFEYLRNYYPVRREFSAYTLHTKGLNHELRLKLNQLGFDGG"
     gene            complement(1301..2413)
                     /locus_tag="PKM34_09905"
     CDS             complement(1301..2413)
                     /locus_tag="PKM34_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83945.1"
                     /translation="MKKLAITVAILTASFTSGGQQGNPELQKQVDELKARVKQLEKEN
                     IVFIAGNKTEDSLDYALCRAEIYKIFLHLQKLDGDYQHTSDKLAVTGLFSKLIQAGNP
                     TSDILGFRFTDVIMTSVDKHFIQYLKDEKDKKRLINVIGKIMDNPVVSNLANTNPVTS
                     VIASVIHIVSDFSTTSVELKKNGRKVTGAGVNEQDIFDSPSIKNFRASLQIYIDFYDV
                     LNQITSEFVKSLDDLNKEYHTLNTRISLFKSDLYNTLNVNDENLFLRLSQALPDPSAD
                     RINNEIMITRAVVKDCYRFSDRFAVLREDADEYEIRYKTLLYSFLDDYIKTLGLINDF
                     PDSSIDKSRARELADEIKCYIKSRKLVDAVLLNVDE"
     gene            complement(2458..>3132)
                     /locus_tag="PKM34_09910"
     CDS             complement(2458..>3132)
                     /locus_tag="PKM34_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNQ83946.1"
                     /translation="ANKSEDVSDVLVKFMIRKPDFLPYGLYQESVYLKVYKMFQEQIL
                     SDFRLLDTNEIEIPGYYYMYERSGTHEHNMEMDSAIAVEPDTSTVYLSDLDLFTLEGD
                     KFEMYADGLVLLDLWYVGCAPCMKSAPVLEKLYKQYGSMVHFLSINEVDRDTARIKLF
                     KEKMGITVPALLGGNNKIANRVSQYRGYPVFILFDASTGKVLWHIAGYSEELEKVIGE
                     VIEKNL"
BASE COUNT          851 a          706 c          634 g          941 t
ORIGIN      
        1 ccgcggcgag gtcaacgatc ggtttgctca tcgcaaagtt atattttggt taagatttgc
       61 gataaaaata gatcagaatt cgggagcggt tcaagtatcg ttgataaatt ctgaaaaaaa
      121 catgcggcta tccaccatca aatcccagct ggttgagctt cagcctaagt tcatgattta
      181 agcctttggt gtgcaatgta taagcgctga attcacgccg cacagggtag tagtttcgca
      241 gatattcgaa ctgcaacgga tcatttctca atgtcctgtc atcttttaaa acatcataag
      301 tgcccagaat agcttcatcg atgatccgct cttctgactt acctgctgat ttaatctcaa
      361 ttacgggttc tcctggccca ggtagatttt ccggatacca ttcatcgata ccaaaaccga
      421 aataccggga ggctgccctg accacggctg ctgtgccgtt ggcttttcct tcgaccgaat
      481 aaccggcgat gtgaggcgtt gcaatatcgg ctattgcaag caattccctg tcgatatgcg
      541 gttcatcctc ccagacatcg gcaacgaaac cccggatcat gcccttttga tgcgccactt
      601 ttaatgcgga accatctaca accgctccgc gagaagtgtt gataatcaat ggcttcttag
      661 ccaggctatt gaaaaaggca tcgtttgcca gatgaaaagt ccggtcatca cctccaaggt
      721 taagcggaac atggaaagta attaaatcac actcctcttt gatcttttgc agcgaaacaa
      781 aaccctgttg cccttcgttt ctggccctgg gcggatcatt gagcaatggg ctcattccca
      841 atatttccgc caataatgcg attttctttc cgacattgcc tgcgccgata ataccaattg
      901 tttttcctgc aagactgata tgatattttg ttgagagcct tgaaaaggca gaagccatat
      961 actgcatgac cgatccggaa ttacagccgg gagcgtggta ccattcaatg cttcggttat
     1021 tacagtattg aatatcaata tgatcggttc caatggtggc ggatgcgatg aatttaaccg
     1081 gtataccgtc aagcaattca gcattgcatc gggtccgggt ccttatgatc aggccgtcgg
     1141 ctttggatgc aatatctttg ctgattacgc cgcccggagc ataagtcact cttgcgaatg
     1201 gttccagaac acctttaatg aacggaatat ggtcatctat tataataacc ggatgtttca
     1261 aatataaaag ctatttatct tttgacaaaa ttaaaacaat tcattcatcc acatttaaga
     1321 gaacagcatc tactaatttc cggcttttta tatagcattt tatctcatct gcaagttccc
     1381 gggccctgga tttatcaata ctggaatcgg gaaaatcgtt aattaaacca agagttttaa
     1441 tatagtcatc cagaaaactg tataacaaag tcttatatcg gatctcgtat tcatcggcat
     1501 cctctcttaa tacggcgaac ctgtctgaaa accggtaaca atctttaacg actgcccggg
     1561 taatcattat ttcattattt atccggtcgg ccgagggatc gggaagggct tgtgataacc
     1621 ggagaaacag gttctcgtca ttaacattta gcgtgttata caaatcactc ttgaataacg
     1681 agatccgggt attcaacgta tggtattctt tattaagatc atccaggctt tttacgaact
     1741 ctgatgtgat ttggttcaat acgtcataaa agtcgatata tatctgcaat gaggcccgaa
     1801 aatttttgat ggacgggctg tcaaagatat cctgttcgtt gactccggct ccggtgactt
     1861 ttctgccgtt ttttttcaat tccactgaag ttgttgaaaa atctgaaaca atgtggatta
     1921 ccgatgctat gaccgatgtc accgggtttg tattagccag gtttgaaacc accggattgt
     1981 ccattatttt cccgatcacg ttgatcagtc ttttcttatc tttctcatct ttaagatatt
     2041 gaataaagtg tttatcgacc gaagtcataa taacatccgt aaaacgaaag cccaggatgt
     2101 cgctggtggg attgccagcc tgtatcaatt tggaaaataa acctgtcacc gctaatttat
     2161 ctgatgtatg ctgataatcg ccatcgagtt tttgcaggtg caggaatatt ttatagattt
     2221 ctgcacggca cagtgcataa tccaggctgt cttccgtttt atttccggca ataaaaacaa
     2281 tattttcctt ttcaagctgt ttgaccctgg cttttaattc atctacctgt ttttgcaact
     2341 ccggattacc ttgctggcct ccggaagtaa atgaggcagt gagaattgct acagttatgg
     2401 ccaatttctt catacacaat attcttcccc attgccatgt tgaaatagtg aaacggttta
     2461 aaggttcttt tcgatcacct ctccgataac tttttccagt tcttcggaat atcctgcaat
     2521 atgccacaat accttcccgg tagaagcatc gaatagaata aataccggat atcccctgta
     2581 ttgtgaaacc cgattagcga ttttattatt acctcccagc aaagcaggaa cggtaattcc
     2641 cattttttcc ttaaagagtt tgatacgggc ggtatcccgg tcaacctcgt tgatgctgag
     2701 aaagtgaacc atactgccgt attgcttgta aagtttctcc agcaccggtg ccgacttcat
     2761 gcagggagcg cacccgacat accagaggtc aagaagaacc agcccgtcag catacatctc
     2821 aaatttatcg ccttccagag taaaaagatc caggtcagac aaataaacag tacttgtatc
     2881 gggttccacc gcaatagccg aatccatctc catgttatgt tcgtgcgtgc cggacctttc
     2941 atacatgtaa taatatccag ggatttctat ctcattagta tccagcagtc taaagtcaga
     3001 tagtatttgt tcctggaaca ttttatagac tttcagatat acggattcct ggtacaatcc
     3061 atagggcagg aaatcgggtt ttcttatcat gaatttaacc aggacatcgg agacgtcctc
     3121 ggatttgttg gc
//
LOCUS       DAOQXS010000374         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_32981_length_3130_cov_3.470244, whole genome shotgun sequence.
ACCESSION   DAOQXS010000374 DAOQXS010000000
VERSION     DAOQXS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32981_length_3130_cov_3.470244"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKM34_09915"
     CDS             <1..746
                     /locus_tag="PKM34_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNQ83947.1"
                     /translation="PREIRKLSVIAEKQRNVKPTWSLGAYFNPEMTSYSDESLENSIG
                     FNLAILPTISFSHFYVQSGFNARFTHDKGDYAIDFNRYLGSYEDVYEVTFDSTENGVI
                     PIYHTQTVDVYDTVDHYSISETKATYTYLEVPVLFGYRHSFGRFGIRAQAGPAASFLI
                     LKDIPAASVPEENARIVNVNSLIPVRSTINWQLVVGAGFDYELADRVIFSLEPTFRYA
                     LDPDFTMPENRNGRTHSFGIRAGLNYRFK"
     gene            753..2366
                     /locus_tag="PKM34_09920"
     CDS             753..2366
                     /locus_tag="PKM34_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ83948.1"
                     /translation="MKRIMNIIQKQYRTKAIICAAVVTLMSFIVLPVSSQIIAGQKNI
                     CLSGQLTSTNTGAPIADHEIYITSDSLTNGGFGYYAVTRTDVNGFYWDTLTTTTNDGI
                     IKIYVYDFDNNRIELDRYYRFIWENQYLMFADFAIFDPEANPDLQANFIPTPDTLDDN
                     PMKIIFRDQSIGSSIKSWYWDFGDGKSSTVQDPDHIYDASGIYMVSLTITTMPPEYGV
                     SESSTITKQVQVGLREFHHLGGHVFASYFPIDYGLAYLYLYDDHNNLIPVDTTRIDTL
                     GYYFFYEVPVGKYLTKTRLESNSVHYGQFMPTYFRNAIDWNDATPIIVDDNDNWECDI
                     NLRPSAGLGAGSGEILGQISYDTSLVVRTLVPAGNIEIVLMDAKGGCLTCTLSDMEGY
                     FLFNDLAYGTYQLFPDVAGVTSTPMYITISEENPALDDISLVIYPGEITFSINENVSD
                     YVGNAMMVYPNPVNDQARLSLEIKKNTTATVVITDIAGRMVYRQETRLSEGMQNIVIP
                     VRDLQKGIYQVTLIPEDRVLVSGKFLKMD"
     gene            2457..3047
                     /locus_tag="PKM34_09925"
     CDS             2457..3047
                     /locus_tag="PKM34_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ83949.1"
                     /translation="MNSDEQIISGCREGKRKAFSKLFDTYAPVMMGVCMRYCKNRIDA
                     EDVMQDGFIKVFGQIHKFRGDGSLEGWIKRIMINAAIDSYQANLKHAFHEDVTENKLS
                     AQIADMDDHCDDDLPPELQIPHKKLMEMIQELPDGYRMVFNLFAIENYSHKEIAALLG
                     ISENTSKTQLLKARKALRRKIEAIIEHSHINRISTV"
BASE COUNT          846 a          785 c          733 g          766 t
ORIGIN      
        1 ccccgagaga aatcaggaaa ttgtcggtca tagctgaaaa acaacgtaat gtaaaaccga
       61 catggtcgct cggtgcatat ttcaacccgg aaatgaccag ctacagcgat gagagcctgg
      121 aaaattcaat cggatttaac ctggcaatcc tgccgactat cagcttcagc cacttctatg
      181 ttcagagcgg attcaacgcc cggtttacac acgataaagg agattatgcc atcgatttca
      241 accgttattt ggggtcttat gaagatgttt acgaagtaac cttcgattca actgaaaatg
      301 gtgttatccc tatctatcat acccagacag tcgatgtata tgatacagtg gatcactaca
      361 gcatctccga aacaaaagcc acctatacct atctggaggt cccggtgctg tttggttaca
      421 ggcattcgtt cggaaggttc ggaatcaggg cacaagccgg cccggctgct tccttcctca
      481 ttctgaaaga tatccctgct gccagtgtgc cggaagagaa tgcaagaata gtcaatgtca
      541 attccctgat accggtccgt tcgaccatca actggcagct cgtcgtgggc gccggatttg
      601 attacgaact ggccgacaga gtaatattca gccttgaacc cacgttccgt tatgccctcg
      661 atcccgattt caccatgcct gaaaacagga acggcaggac gcattcgttc ggaataaggg
      721 caggcctgaa ttacaggttt aaataattga tcatgaaaag gataatgaat atcattcaga
      781 agcaataccg cacgaaggcc atcatctgcg ccgccgttgt gacgctgatg agtttcatcg
      841 tccttccggt ttcatcccag atcatcgccg ggcaaaagaa catctgtctg agcggacagc
      901 tgaccagcac caatacaggg gcgcccatcg ccgaccatga aatatacatt acctccgatt
      961 cactgaccaa cggaggcttt ggctattatg ccgtaacaag gaccgacgtc aacggttttt
     1021 actgggatac ccttaccact actactaacg acgggattat caagatctat gtgtatgatt
     1081 ttgataataa caggatcgaa ctcgaccggt actaccggtt catctgggaa aaccagtacc
     1141 tgatgttcgc cgatttcgcc attttcgatc cggaagccaa cccggacctc caggctaatt
     1201 ttataccaac cccggatacc ctcgacgata acccgatgaa gatcattttc agggaccagt
     1261 cgatcggcag cagcatcaag tcgtggtact gggattttgg tgatggcaag tcttccaccg
     1321 tccaggatcc tgaccatatc tacgatgcat cgggcattta catggtttca ctgacgatca
     1381 ccacgatgcc gccggaatat ggcgtttccg aatcgagcac catcaccaag caggttcagg
     1441 tcgggctgcg cgaattccac cacctgggcg ggcatgtgtt cgccagctac ttcccgatcg
     1501 attatggtct ggcttacctc tacctgtatg atgatcacaa caatctgatc ccggtagata
     1561 cgacacggat tgataccctg ggatattatt tcttctatga ggtcccggta ggaaaatatc
     1621 tcaccaagac ccggctggag tcgaattcgg tgcattatgg ccagttcatg ccaacctatt
     1681 tccgcaatgc catcgactgg aacgatgcca ctcctatcat tgtcgatgat aatgataact
     1741 gggaatgtga tattaattta cgtccgtcgg caggattagg cgctggttcg ggcgagattc
     1801 ttggccagat atcctatgac acctcgcttg tagtcagaac cctggtccct gcaggtaata
     1861 ttgagattgt tctcatggat gccaaagggg gctgcctcac ctgtaccctg tcggatatgg
     1921 aagggtattt cctgttcaac gacctggcat acgggacata ccagttgttc ccggatgtag
     1981 ccggcgttac ttccaccccg atgtatatca ccatctccga agaaaatccg gcactggatg
     2041 atatcagcct ggttatttat cccggagaaa taaccttctc catcaatgaa aatgtttcgg
     2101 attatgtagg aaatgccatg atggtttatc cgaacccggt gaacgaccag gccaggcttt
     2161 ccttagaaat aaagaaaaac acgacagcta ctgttgtcat caccgatatt gccggccgga
     2221 tggtttaccg ccaggaaacc cggctctccg aaggaatgca gaatatcgtc atccccgtaa
     2281 gggatctgca aaaaggaatt taccaggtca ccctcatccc ggaagaccgg gtgctggttt
     2341 caggcaaatt cctcaaaatg gactgatgtt ttttcccgat tatttttggt atttacagca
     2401 cctccgggca acttttctta tttattcccg tctgacttaa tgtaagatct tacaggatga
     2461 attccgatga gcaaatcata tccggttgcc gtgaaggcaa gcgaaaagct ttcagcaagc
     2521 ttttcgacac ctatgcaccg gtgatgatgg gtgtttgcat gcgatactgc aaaaaccgga
     2581 tcgatgcgga agatgtgatg caggatggat ttatcaaggt attcggccag attcacaaat
     2641 ttcgcggcga cggctccctt gagggatgga tcaaacggat catgatcaat gccgctatcg
     2701 acagttacca ggccaatctt aagcacgctt tccacgagga tgtgacggag aataagctca
     2761 gcgcccagat agccgatatg gatgaccact gtgatgacga cctgccgccg gaattacaga
     2821 tcccgcataa aaaactgatg gaaatgatcc aggaactgcc cgacggttac aggatggttt
     2881 tcaacctctt cgccattgaa aattacagtc ataaggagat tgccgctctg ctggggattt
     2941 cggagaatac atcgaaaacc cagcttctga aagcgagaaa agcactccgc cggaaaatag
     3001 aagccattat agaacacagt catatcaaca ggatatcaac ggtatgaaca atatgaacaa
     3061 aatagacgat cttttcaggg aagccatgac cggctataag atgacgcctg cccccggtct
     3121 ctggctgagg
//
LOCUS       DAOQXS010000375         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_33111_length_3122_cov_3.109227, whole genome shotgun sequence.
ACCESSION   DAOQXS010000375 DAOQXS010000000
VERSION     DAOQXS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33111_length_3122_cov_3.109227"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PKM34_09930"
     CDS             complement(<1..1523)
                     /locus_tag="PKM34_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ83950.1"
                     /translation="MAPIHNRNLKIPHFHSLRVYLVSTILYFFLVLPVASILALKYYI
                     PDYLYQKSEPAAIANPDISTDRQVYDPTPDTLGKTVDSIRRAENNSLVTVRFSTPDSP
                     QFQITTGENTSENSRFGGTISVLMKLLFVSFILGLFFNLPFKIYFSKKRNLKPIKPGV
                     FQYCRRLILKVPLINTGILALPYLITLLYMLYFILFRRDEDPLDRQFYTQYFFITLVA
                     GILTLMLVFFWLKHRVHIRYLEHIYSPDELKKRIFKGKPGRIKNRLWISSAMTTLFPL
                     IIVVFYIVLSISSVEELSLLNLSDENQKILLGKYIAFDLGLDLPTLNEFFYVNAVNSF
                     LMVVGIGTGVFIALIYVVLFVRWTTEDIIYPVNELLANMQRTGRGELDIYSIVRTNDE
                     VGALTEGYNEMTGKLKSYFESLDKINRSNARFVPRQFLDFLGKENITEIQLGDQVQQE
                     MTIMFSDIRDFTSISESMTPKENFDFINNYLGYMEPVIRTNNGFIDKFMGDSIMALFP
                     "
     gene            complement(1583..2128)
                     /locus_tag="PKM34_09935"
     CDS             complement(1583..2128)
                     /locus_tag="PKM34_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ83951.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKIMAYSGIMQDQEVSWMPSYGPEMR
                     GGTANVTVILSDERISSPILTRYDTAIILNQQSMDKFEPMVKPGGTLIYDANGITRHP
                     ERKDIKIYRVDAADEASKMGLSKIFNMIVLGGFLRIRPIISLENVIQGLKKSLPARYH
                     HLIPDNEKALKRGMEIIAQVN"
     gene            complement(2165..2956)
                     /locus_tag="PKM34_09940"
     CDS             complement(2165..2956)
                     /locus_tag="PKM34_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ83952.1"
                     /translation="MDEKLYNEIVAEENLVYKKTSLMTEKVLSYCPGCGHGTAHRIIM
                     EVLDEMGLQENTIGVAPVGCSVLAYEFMNVDMQQAAHGRAPALATGIKRTWPNKVVFT
                     YQGDGDLAAIGTAETIHACNRGENIIIFFINNGIYGMTGGQMAPTTLPNMKSSTSPYG
                     RDTQIMGHPLKITELVAQLPGTYFVTRQAVHTPANVRKTKKAVRMAFENQLGNKPGLS
                     FIEIVSNCNSGWKMTPVQANQWMVDNMFPFYPLGDIKVDGALVAK"
     gene            complement(2961..>3122)
                     /locus_tag="PKM34_09945"
     CDS             complement(2961..>3122)
                     /locus_tag="PKM34_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNQ83953.1"
                     /translation="GILTVEMSAGQMVEDVLLAVAGKVKVEHYGRMGGMIPSPEDVVT
                     AMEKKIIGG"
BASE COUNT          789 a          737 c          746 g          850 t
ORIGIN      
        1 ggaaagagcg ccataatgga gtcgcccatg aacttgtcga tgaaaccgtt atttgtccgg
       61 atgaccggtt ccatatatcc caggtagttg ttgataaaat caaagttctc ctttggggtc
      121 atcgattccg agatagaggt gaaatcacgg atgtcggaga acatgatggt catctcctgc
      181 tgaacctggt cgcccagttg gatttcggta atattttcct tgccgagaaa atcgagaaac
      241 tgccggggta caaagcgggc attcgaccgg ttgattttgt caaggctttc aaaataactt
      301 tttaattttc cggtcatctc attgtagcct tccgtcaggg cgccaacttc gtcattcgtt
      361 ctgacaatgc tgtagatatc cagttcgccg cggcctgtcc gctgcatatt ggccagcagt
      421 tcgttaacgg ggtagattat atcctccgtt gtccatctga caaacagaac gacatagatc
      481 agcgcgatga atacgccggt cccgataccg acaaccatca ggaagctgtt cacggcgtta
      541 acatagaaga attcattgag cgtgggcagg tcaagtccca ggtcaaacgc tatgtatttg
      601 cccagcagga ttttctggtt ttcgtccgaa aggtttagca ggctaagttc ttcaaccgag
      661 gagatgctca ggacaatata aaatacaacg ataatgagcg gaaacagggt ggtcatggcg
      721 ctggaaatcc agagccggtt tttaatcctc cccggcttgc ctttaaagat ccttttcttt
      781 aattcatccg gagaatatat atgttcaaga tatcggatat gaacacggtg tttgagccag
      841 aagaacacaa gcatcagagt cagtattcct gcaaccagtg tgatgaaaaa gtactgcgtg
      901 taaaattgcc ggtccagcgg atcttcatct cttctgaaca aaataaaata aagcatatac
      961 agcagtgtga tcaggtaggg aagggcaagg atacccgtat tgatgagggg gactttcaaa
     1021 atcagccggc ggcaatattg aaacacgccg ggtttaatgg gtttgagatt tctttttttg
     1081 ctgaaataga ttttgaaagg aaggttgaaa aaaagaccca gtataaagct cacaaaaagc
     1141 agcttcatca gaacggagat tgtgccaccg aaacggctgt tttcgctggt gttctcaccg
     1201 gtagttattt gaaactgcgg tgaatcgggg gtggagaacc tgacggtgac cagagaatta
     1261 ttctccgccc ggcgaatgga atctacggtt ttaccaaggg tatcaggtgt cggatcatac
     1321 acctgacggt ctgtagaaat atcaggattt gcgattgcag caggctcact tttctggtaa
     1381 agatagtcgg gtatataata tttcaatgca agaatactgg ccaccggcag gaccagaaaa
     1441 aagtagagga ttgtggaaac caaatatacc ctgaggctgt gaaaatgggg tattttcagg
     1501 ttcctgttat gaattggcgc catgcaaaca gtattatgca agcgctaaag tataaaaaat
     1561 tgtgattgag tctgacaggg ttttagttca cctgcgcgat gatctccatg cctcttttaa
     1621 gggctttttc attatccggg atcaaatggt gataccgggc cgggagtgat ttcttcaatc
     1681 cctggatcac attttccagc gaaatgatcg gcctgatccg gagaaaccct cccaggacga
     1741 tcatgttgaa gattttggac aaacccattt tcgaggcttc atcggctgca tcaacacggt
     1801 aaatctttat atctttcctt tcgggatgcc gggtgatgcc gttggcgtcg taaatcagcg
     1861 ttccgcccgg cttcaccatc ggttcgaact tgtccatcga ttgttggttc aggataatcg
     1921 cggtgtcgta ccgggtcagg ataggggagc tgatcctttc atcgctgagt atcaccgtta
     1981 cgtttgctgt tccgccgcgc atttccggcc cgtaggaggg catccagcta acctcctggt
     2041 cttgcatgat accggaatag gccatgatct tacccatcga cagtacaccc tgtccgccaa
     2101 aacctgctat gataatttct tctgtcataa tttattaatt tgactaacga ccaatgacta
     2161 atgactattt cgcgaccagc gctccatcca cctttatatc gccaagcgga tagaaaggga
     2221 acatattatc caccatccac tggttagcct ggacgggtgt catcttccac cctgagttac
     2281 agttggatac aatttcaatg aatgataagc ccggcttatt gcctaactgg ttctcgaatg
     2341 ccattctgac ggcttttttg gtttttctca cattagccgg agtatggaca gcctgccggg
     2401 taacaaagta agtacccggg agttgagcca ccagttcggt aattttcagc ggatgtccca
     2461 taatttgagt atcccttccg tacggagaag tggacgactt catattgggc agcgtggtgg
     2521 gcgccatctg gccacccgtc ataccatata taccattgtt aataaagaag ataataatgt
     2581 tttcaccccg gttgcaggcg tgaatggttt ctgcggtacc gatggcagcc aggtcgccgt
     2641 cgccctggta tgtgaaaacg actttgttcg gccatgtacg cttgatacct gtggccaggg
     2701 caggggcccg gccatgcgcc gcctgttgca tatccacatt catgaattca taggccagaa
     2761 cggagcaacc gaccggtgca acgccaatgg tgttttcctg caaacccatt tcgtccaaca
     2821 cttccatgat gatccggtgg gccgtcccat gcccgcatcc cgggcagtag ctcagcactt
     2881 tctcggtcat caggctggtt ttcttataaa ccaggttttc ttcggctacg atttcgttat
     2941 ataatttttc gtccataatt tcaacctccg attatttttt tctccatagc ggtcactaca
     3001 tcttccggtg atgggatcat tccgcccata cgaccgtaat gttcaacctt aaccttgccg
     3061 gctacggcca gcaatacatc ttcaaccatc tggccagcgc tcatttccac tgtcagtatc
     3121 cc
//
LOCUS       DAOQXS010000376         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_33516_length_3097_cov_3.536160, whole genome shotgun sequence.
ACCESSION   DAOQXS010000376 DAOQXS010000000
VERSION     DAOQXS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33516_length_3097_cov_3.536160"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..500)
                     /locus_tag="PKM34_09950"
     CDS             complement(108..500)
                     /locus_tag="PKM34_09950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNQ83954.1"
                     /translation="MKIYFNGNKQVFADFNEFTVKTDQSARGGGDGLYPEPFALFLAS
                     LGTCAGIFVKSFCDQRGIPTEKIHLTMEPKFDPVRHMIGEFDMTIHVPADFPEKYDQA
                     VIQTASLCTVKRHLRDDIRFNVVVNRLS"
     gene            624..2039
                     /locus_tag="PKM34_09955"
     CDS             624..2039
                     /locus_tag="PKM34_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ83955.1"
                     /translation="MTIQGEIFRNFPFEPTRGQQNLADELAKFIGDPEPWSVFILKGY
                     AGTGKTSFVSAMVRILPVIRKKAVLLAPTGRAAKVLSGYAGQPAWTIHKRIYFQRVGR
                     DGSMNLVLQKNLYRNALFIVDEASMIAGNQAERDQLFSSRNLLDDLVEYVYSGESCRL
                     LLIGDTAQLPPVGMAISPALDLDYLKSRFNLKIFRHELTEVMRQSLESGILSNATALR
                     QKLAAGETTLPLFAINGMRDILTVPGNELEELFHSAFAPDRISGSVVICRSNKRANLF
                     NKEIRNRILFRENELSAGDHLMVVKNNYYWLDPDSAPGFIANGDIIELQNVRNMEDLY
                     GFRFADVTVRMIDYPLEPAFDVKILLDVLMAESPSLPDLRQKELFGKVLEEVNDIPQK
                     SVRLEKVRSNPYYNALQVKFAYALTCHKTQGGQWENVFIDQGYLKAEQVDAEYLRWLY
                     TALTRATQKVYLLGFMENFFI"
     gene            2122..2883
                     /locus_tag="PKM34_09960"
     CDS             2122..2883
                     /locus_tag="PKM34_09960"
                     /inference="COORDINATES: protein motif:HMM:NF037852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83956.1"
                     /translation="MKNLVILSQLVIIVFFSQNIFSQVAINTSGADPDPSAVLDINAP
                     DKGLLIPRVTLLSKVNNNAPIQNPATGLMIYNLGGNNLDAGFYIWNGMTWSALATVEQ
                     VMNLANAPQPAAVFGELYEYHNIGDFTTIQVASGGTWMPWNTAQEGDTSEMAASASTL
                     TILNPGTYTVSFNAALQMSSGGKITEAALFVNGIRQDDMRTRCWFKEGSKAQNFSFSG
                     LIELNEGDVVSVKFTVNENGTIRLEMANLNLTRLN"
     gene            complement(2897..>3097)
                     /locus_tag="PKM34_09965"
     CDS             complement(2897..>3097)
                     /locus_tag="PKM34_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83957.1"
                     /translation="TGFFYEDKTKGNRKFITVGAGLRYSVFGLDLSYLIPFEQQHPLE
                     NTLRFTLHFNFDPDKESDLPRK"
BASE COUNT          809 a          781 c          741 g          766 t
ORIGIN      
        1 tcttgtcatg ctcgaaaccc tgtcagaaca ggccgaaccg gtggctgccg caaaaaagat
       61 tgccctcctt tgagagccgg caatcctctc atgcaactct atcggattca tgacaatcga
      121 ttcacaacaa cattgaaccg gatatcatca cgcaggtggc gtttcaccgt gcaaaggctg
      181 gccgtctgga tgaccgcctg gtcatatttc tcaggaaaat cagccggaac atggatggtc
      241 atgtcaaact ctccgatcat atgacggaca ggatcgaatt tgggctccat cgtcaagtgt
      301 atcttttcgg tgggtattcc cctctggtcg cagaaagatt taacaaagat acctgcgcag
      361 gttccaagag aagccaggaa aagggcaaaa ggctccgggt aaaggccgtc gcctcctccg
      421 cgggctgact ggtcggtttt tactgtaaac tcattaaaat cggcgaaaac ctgtttgttt
      481 ccgttaaaat aaatcttcat agtatgtcga tatttaagtt aaaagatgcg gttgtcagca
      541 atttccaggc cagcggaata ttcgtgtccc ggtgatgaca ttatggcacc ccgatccggg
      601 ggatttgtta attttgggcc acaatgacta tccagggaga aatattccgc aattttccat
      661 ttgagcctac gcggggccag caaaacctgg ccgatgaact ggcgaaattt atcggcgatc
      721 ccgagccctg gtcggttttt attctgaaag gctatgccgg aaccgggaaa accagttttg
      781 tcagcgcaat ggtacgaatc cttccggtta tccgcaaaaa agctgtcctc ctggccccga
      841 ccggaagggc tgccaaggtt ctctctggct atgccggcca gcctgcgtgg accattcaca
      901 aacggatcta tttccagcgt gtcgggcgcg acgggagcat gaacctggta ttgcagaaga
      961 acctgtaccg gaatgccctg tttattgttg atgaagcctc catgatcgcc ggtaatcagg
     1021 ctgaacgcga tcaactgttt tcatcacgga acctgctgga tgacctcgta gaatatgttt
     1081 actccggtga atcctgccgg ctgctgctga taggcgacac agcccagttg cctcccgtcg
     1141 ggatggctat cagcccggcc ctcgacctgg attacctgaa atcgaggttt aacctgaaga
     1201 tctttcgcca tgaactgacg gaagtcatgc ggcaatcgct cgaatcgggc attttgtcga
     1261 atgcaacagc gctcaggcag aaacttgccg ccggtgaaac cacactcccg ctgtttgcca
     1321 tcaatggaat gcgcgacatc ctcacagttc cgggcaatga actggaagag ctctttcaca
     1381 gcgccttcgc ccctgaccga atctccggat cggtcgtgat ctgccggtcg aataaaaggg
     1441 ctaacctgtt caacaaagaa atccggaacc ggatcctctt cagggaaaat gaattatcag
     1501 ccggagatca cctgatggtc gtcaaaaaca attactactg gcttgacccg gattccgcac
     1561 ccggttttat tgccaacggt gatatcatcg aactccagaa tgtcaggaat atggaagacc
     1621 tgtatggttt ccggtttgcc gatgttaccg tcagaatgat tgattatccc ttagaaccgg
     1681 cgtttgatgt gaaaatcctg ctggatgtcc tgatggctga atcaccatct cttcccgatt
     1741 taagacaaaa agaactcttt ggcaaagtcc tcgaagaggt caatgatatc ccgcaaaaat
     1801 cggtcaggct ggaaaaggtc aggtcaaatc cttattacaa cgcgctacag gttaaattcg
     1861 cctacgccct cacctgccac aaaacgcagg gcggacagtg ggaaaatgtc tttatcgacc
     1921 aggggtatct gaaagcggaa caagtggatg ccgaatacct ccgttggctt tatacagccc
     1981 ttaccagggc cactcaaaaa gtatatcttc tcgggtttat ggaaaatttt ttcatataaa
     2041 tgtaaaagca ataaattaaa tactaccttt gcaccgcttt tttagaccga accatctgct
     2101 aaacccaaaa cccccaaggt catgaaaaac ttagtgatac taagccagtt ggttattatt
     2161 gtattctttt cgcagaatat cttttcgcag gttgccatca atacatcggg tgccgatccc
     2221 gatcccagcg cagtcctcga tattaacgct cctgacaaag ggctcctgat ccccagggtc
     2281 accctcttaa gtaaagtcaa taacaatgct ccgatccaaa atccggcaac cggattgatg
     2341 atttataacc tgggaggaaa taacctcgat gcaggctttt atatctggaa cggtatgaca
     2401 tggtcggccc tggccaccgt tgaacaggta atgaatttgg caaatgctcc gcagccggca
     2461 gccgtttttg gcgaattata cgagtaccat aatatcgggg atttcacaac aattcaggtc
     2521 gcttcaggcg gaacctggat gccctggaat acggctcagg aaggagacac cagtgaaatg
     2581 gccgcttctg cttccacttt aacgatttta aacccaggca catataccgt ctcattcaat
     2641 gcggcgctcc agatgtcgag cggtggcaag atcactgaag cagcgctctt cgttaatggt
     2701 atccggcagg atgacatgcg cacccgctgc tggttcaaag aaggcagcaa agctcagaat
     2761 ttttcgttct caggattgat tgaactgaat gaaggcgatg tggtttccgt taaattcacc
     2821 gttaatgaaa acggcacgat ccgcctcgaa atggcaaacc tcaatctgac ccgcctgaat
     2881 taattgttcc cggtttttat ttcctgggga gatcgctctc tttatccgga tcaaagttga
     2941 aatggagggt gaaccggagc gtgttttcca atggatgctg ctgttcaaag gggatcaggt
     3001 acgacagatc cagaccgaaa acactgtagc gcaagcctgc accgaccgtg ataaatttcc
     3061 ggttgccctt ggtcttgtct tcataaaaga atcccgt
//
LOCUS       DAOQXS010000377         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_33689_length_3086_cov_4.237875, whole genome shotgun sequence.
ACCESSION   DAOQXS010000377 DAOQXS010000000
VERSION     DAOQXS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33689_length_3086_cov_4.237875"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKM34_09970"
     CDS             complement(<1..1033)
                     /locus_tag="PKM34_09970"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNQ83958.1"
                     /translation="MKANTEMNPNPLVQYLNKPAEEFTRQDIIKYVEDNNIEMINFRY
                     VGWDGRLKTLNFIINGRDHLETILTMGERVDGSSLFSFIEAGSSDLYVIPKYRTAFIN
                     PFTEIPTIDIICSYFNKDGEPLESSPEYILKKADRALKEKTGYELEVMGELEYYIISP
                     KDDLFQAVDQRGYHESTPFTKWEHLRSEAMQAIAQAGGQIKYGHSEVGNFEVGDLMYE
                     QNEIEFLPCPLESAAEQLLIAKWILRSLAYKYGVTISFAPKITLGKAGSGLHIHTRLM
                     KDGKNVMIENGRLSDTARRMIAGFLDLAPSLTSFGNINPTSYFRLVPHQEAPTNICWG
                     DRNRSVLVRV"
     gene            1167..2219
                     /locus_tag="PKM34_09975"
     CDS             1167..2219
                     /locus_tag="PKM34_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNQ83959.1"
                     /translation="MEISNEVIFFSGFIVLILFLLFIDLGVFSKNSHVVSLRESVSWT
                     ILWVSLSIGFYFFIRHYGNLIHGYDSIADIQASIDKFKHPINIEGLTFDQALQVFNKN
                     LSIQYITGYIIEYSLSLDNVFVILLIFLSFNVPEKYYKRVLFWGILGAIIMRFIFIFS
                     LSAIIYKFSWVMYIFGLFLVFTAIKMFLDFIKKKEEKIDVEHHPMVRLTKRFFPVHPK
                     FEGNKFWIRKNRIVYVTPLFIALMVIEFSDLIFAVDSVPAIFAVTRDPYIVFFSNIFA
                     IMGLRSLFFLVSHFFSKFFYLKVGLAALLAFIGLKMLTHDFYKISTDASLIVISAILG
                     LSIIASLVHNKREQQV"
     gene            complement(2235..>3086)
                     /locus_tag="PKM34_09980"
     CDS             complement(2235..>3086)
                     /locus_tag="PKM34_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83960.1"
                     /translation="SPFLDVPANTQVTIAIKGADSQDPSNPLWSKSYTFTEDEKYILV
                     AGGILSPTGYDPQKPFTVFDYEGALEQAAETTNTDVLVFHGVTDALLVDVVVVGDGIG
                     TIVDDLDYGLFDGYMQLETGNYLLSVRDESGTTEVAYFETNFQDLNLQGKSLTLLASG
                     FLDPTANNDGPAFGLLGVKADGSTVLFTNTLGIPEPSVNLTGLNVFPNPANDYFNVTF
                     VMMSKGIVTVELIDIMGRKAAEHYFGELNAGKVNEKITLDGIQDGYYLLRINAGDDQV
                     VRRLIIK"
BASE COUNT          742 a          754 c          707 g          883 t
ORIGIN      
        1 gcaccctgac cagcaccgag cggttccggt cgccccagca aatgttggtc ggcgcctcct
       61 ggtgcggcac caggcggaaa tacgaagtcg gattgatgtt cccgaacgag gtgagcgacg
      121 gggcaaggtc caggaacccg gcgatcattc tccgggcagt atcgctcagc cgtccatttt
      181 cgatcatcac atttttcccg tccttcatca gccgggtatg gatatgtagg ccgctgcccg
      241 cttttcccag ggtgatcttg ggcgcaaatg agatcgtgac gccatatttg taagccagcg
      301 accggaggat ccattttgcg atgagtagtt gttctgctgc gctttccagc gggcagggaa
      361 gaaattcgat ttcgttctgc tcatacatca ggtcaccaac ttcaaaattg ccgacttccg
      421 aatggccgta cttgatctgt ccgcccgcct gggcaatggc ctgcatagct tcggaacgaa
      481 gatgttccca cttggtgaaa ggtgtggatt catggtagcc tctctggtcg actgcctgga
      541 agagatcatc tttcgggctg atgatgtagt attccagctc gcccatcacc tccagctcat
      601 accctgtttt ctccttcagg gcccggtcgg ctttcttcag gatatactcc gggctgcttt
      661 ccagaggctc gccatctttg ttaaaatagg aacatataat atcgatagtt ggaatttccg
      721 tgaaagggtt aataaatgcc gtcctgtatt tgggaataac atacaagtcg ctcgatccgg
      781 cttcaataaa agagaaaagg ctcgacccat cgaccctctc acccattgtc aaaatggttt
      841 ccaggtgatc ccggccgtta atgataaagt tcagtgtttt cagtcttcca tcccacccga
      901 catatcggaa atttatcatc tctatattat tgtcttccac atacttgatg atatcctgac
      961 gggtaaactc ctcggctggt ttattcagat actgcaccag cggattcgga ttcatttcgg
     1021 tattcgcttt cattattatc atctttataa ttagtattta acaaaggacg gcaaacatat
     1081 aaatttttga actataaaaa gggccggcca tgtaaattag attgtttcaa aacaataact
     1141 aattttgttg aaatttttgt gtcaagatgg aaatcagcaa cgaggttatt tttttttcag
     1201 gatttatcgt attaatcctt ttccttttat tcatcgacct cggggttttc agcaagaaca
     1261 gccacgtagt atcgctgcgg gagtcggtat catggacgat actgtgggta agcctttcga
     1321 tcgggttcta cttttttatc cggcattacg gaaacctgat acacgggtat gattccatcg
     1381 ccgatataca agccagcata gataagttca agcacccgat aaatatcgaa gggctgacat
     1441 ttgaccaggc actgcaggtt tttaataaaa atttatcgat ccagtacatt acaggttata
     1501 tcatcgaata ttctttaagc ctggataatg tatttgtcat tctgctcatc ttcctctctt
     1561 tcaatgtccc ggagaaatat tacaagcggg tcctgttctg ggggatcctg ggagccatca
     1621 ttatgcggtt cattttcatc ttttccttat cggccattat ttacaagttt tcatgggtga
     1681 tgtatatctt cgggctcttc ctggttttca ccgcaataaa gatgtttctg gatttcatta
     1741 aaaagaaaga agaaaagatt gatgtggagc accacccgat ggtcaggctg accaaaagat
     1801 tcttcccggt tcacccgaag tttgaaggga ataaattctg gataaggaaa aacaggatcg
     1861 tttacgtaac gcccttgttt attgccctga tggttatcga attcagcgat ctgatctttg
     1921 cagtcgattc cgtcccggcc atattcgccg tcacccgcga tccgtatatc gttttcttct
     1981 cgaatatttt tgcgatcatg ggactgcggt cactgttctt cctggtgtcg catttcttca
     2041 gcaagttctt ctacctgaag gtcgggctgg ctgccttgct ggcgttcata gggctgaaaa
     2101 tgctcacgca tgatttctac aaaatctcca cagacgcttc gctgattgtc atatcggcaa
     2161 tactgggact gagcattatt gcctcgctgg tgcacaataa aagggaacag caggtctaag
     2221 accgatgccc gggattattt aatgatcaat ctcctgacca cctggtcatc cccggcattt
     2281 attcttaaca aataatatcc atcctggatt ccgtccaacg tgatcttttc gttcactttt
     2341 ccggcattca gttcaccaaa gtagtgttca gcagctttcc ttcccatgat atcgatcagt
     2401 tcaacagtaa ctattccttt cgacatcatt acaaaagtca cgttgaaata atcgttggcc
     2461 ggatttggaa atacattcag gccggtcagg ttgaccgacg gctcagggat acccagcgta
     2521 ttcgtaaaca gtaccgtact gccatcggct ttgacgccga ggagcccgaa tgcagggccg
     2581 tcgttattag ccgtaggatc caggaagccg gatgcgagta aggtcaacga tttgccttgc
     2641 aggttcagat cctggaagtt ggtctcgaaa taggcgacct cggtggttcc cgattcgtcc
     2701 ctgacactga gcaaataatt gcctgtttcc agttgcatgt agccatcaaa caggccatag
     2761 tccaggtcat ccacgatcgt tccgatgccg tcgccaacta caacaacatc caccagcagt
     2821 gcatcggtca ctccatgaaa gacaagcaca tcggtattgg tcgtttctgc cgcctgctcc
     2881 aacgctccct cgtaatcgaa cacggtgaat ggcttttgag gatcatagcc ggtcgggctc
     2941 aggataccgc cggccaccag gatatatttt tcatcttcgg taaaggtata gctcttcgac
     3001 cacagcggat tggaaggatc ctggctgtcg gctcccttga tggcgatggt tacctgggtg
     3061 ttggccggaa catcgaggaa cgggct
//
LOCUS       DAOQXS010000378         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_33984_length_3067_cov_4.082337, whole genome shotgun sequence.
ACCESSION   DAOQXS010000378 DAOQXS010000000
VERSION     DAOQXS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33984_length_3067_cov_4.082337"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKM34_09985"
     CDS             complement(<1..182)
                     /locus_tag="PKM34_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83961.1"
                     /translation="MNNDLSAADDFRRFAGGKKFATILADPPWQFENRTGKMAPEHKR
                     LARYSTLTLAEIKKIPV"
     gene            complement(172..795)
                     /locus_tag="PKM34_09990"
     CDS             complement(172..795)
                     /locus_tag="PKM34_09990"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNQ83962.1"
                     /translation="MTYTDYIPPDIKSLYEVHDFKHAAAILANEFPGEFEEICDAMRK
                     FRFSLNDIMEAGGSESHIPKIFSGILRPMEWKEKNLSAELKVYEVKGKSSELKSTINH
                     GSHMVDYLKGRVAFDLEWNSKDQTFDRDLYAFRAFFEYDAISVGVLVTRSNELDPLFR
                     KLGIMPKYGASTTHMGKLLPRLEAGRNGGCPVLVFGITTKLITDYEQ"
     gene            complement(859..1437)
                     /locus_tag="PKM34_09995"
     CDS             complement(859..1437)
                     /locus_tag="PKM34_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83963.1"
                     /translation="MAKLYKNRYRIKSARCPNWDYGSKGAYFITICTKGRNHYFGEIF
                     NKTMILSKAGLIVDNCWHELPCHFPNIQIDAFIVMPDHVHLIIIIHQNPNKPGLAEDK
                     TTARNKDGTGEINQVIPEKDGYMSKISPKSGTVSTIVRSYKSACTNAINKSLPAINFG
                     WQPRYYDIIVRDYRSLDRIRRYIRLNPKYWKG"
     gene            complement(1665..>3067)
                     /locus_tag="PKM34_10000"
     CDS             complement(1665..>3067)
                     /locus_tag="PKM34_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNQ83964.1"
                     /translation="LNIREKCIQGRRTGIGITAEGDMLAALGLRYGSEEAISFSTEVH
                     KMLALEACRSSVDLARDRGPFPVYSTEREKNNPFINRIREADPELYEDMKKYGRRNIA
                     MLTIAPTGSVSICTRTSSGIEPVFSVSYRRRRKVNPNDLDVRIDFVDEVGDSWEEYNV
                     FHPKFEEWLKINGYDTEEVKAYHEDELNRLIELSPYNRATSNDIDWVSKVTMQGAIQK
                     WVDHSISVTVNVPKETTEDLVSQIYMTAWESGCKGMTIYRDGSRAGVLIKDEKKKEQD
                     NKEEAQFHETSAPKRPRVLDGEVLHFMNDNQKWVAVIGILDNRPYEIFTGEAEGFVVP
                     DWVKSGQIIKNKLDEHNSRYDFQYQNKYGMKVTLEGLSIQFNAEFWNYAKLISGILRH
                     GMPLPFVVHLVDNLQLESESINTWKNGVVRALKKYIPDGTKVAKEKCPECGEKGTLIY
                     KEGCVTCTACGDSKCG"
BASE COUNT          716 a          785 c          667 g          899 t
ORIGIN      
        1 accgggattt tctttatttc ggccagcgtt aaggtagaat accgcgcaag gcgtttatgt
       61 tccggcgcca ttttaccggt gcggttttca aactgccagg gcggatctgc aaggatggtt
      121 gcaaacttct tcccgccggc aaatctcctg aaatcatccg ctgcagatag gtcattgttc
      181 atagtcagta attaattttg tagtaattcc aaaaaccaat accgggcatc ccccgttccg
      241 tcctgcctcc agacgcggaa gcagtttccc catatgtgtc gtactggctc cgtatttggg
      301 cattataccc agctttctaa aaagcggatc aagttcattg ctgcgcgtaa ccaatacgcc
      361 aacactgatg gcatcatact caaaaaaggc ccggaaggca taaagatccc ggtcgaaggt
      421 ctggtctttg ctattccact caagatcgaa tgcaacgcgt cctttcaggt aatctaccat
      481 atgagaaccg tgattgatcg tgcttttcaa ttcgctcgac tttcctttga cctcataaac
      541 cttcaattca gccgacaggt tcttctcttt ccactccatc ggcctcaata ttccggaaaa
      601 tatcttcgga atatgacttt cgctcccacc agcctccata atatcattca aactaaacct
      661 gaactttcgc atagcatcgc aaatctcttc gaattctccc ggaaattcat tagccaggat
      721 agctgcggca tgcttgaagt catgaacctc gtaaagagac ttgatgtcgg gcggaatgta
      781 gtcggtataa gtcattattc aataccattt taattaattc caaatttaca caaatatccc
      841 gttgggctta tttaattttc atcccttcca gtacttggga ttcagcctga tatacctgcg
      901 aattctatca agcgatctgt aatcccggac aataatatca taatatcgtg gctgccagcc
      961 aaaatttatt gccggcaatg atttgttaat cgcattcgta catgcggatt tatatgatct
     1021 gactattgtt gaaaccgttc ctgactttgg agatattttt gacatatacc catctttttc
     1081 aggtataacc tgattgattt caccagtgcc gtctttatta cgggctgttg ttttgtcttc
     1141 tgccaatcca ggtttatttg gattttgatg aataattatt attaaatgaa catgatctgg
     1201 catgacgata aacgcatcga tctgaatatt gggaaaatgg caaggtaatt cgtgccagca
     1261 attatcaaca atcaaaccag ccttcgacaa tatcatggtt ttattgaaaa tttccccaaa
     1321 ataatgattc ctgcccttgg tgcatattgt gataaaatat gcgccctttg aaccataatc
     1381 ccaattgggg caccgcgctg atttgatcct gtatcggttt ttatacaatt tcgccatttc
     1441 cgatattttt tacatttccg ccattttcgc catttccgcc atttccgcca ttgtagagac
     1501 gttgcatgca acgtctctac aatatcaaca acaacggtta tcaacaacaa cggttatcaa
     1561 caacaacggt tatcgcaata tatcatccat ggcaacgcat tttttaatgc attacgatga
     1621 acaattacaa taaatggttg ggaatgatgg ggataattaa aaccctaccc gcatttcgaa
     1681 tcgccgcagg ctgtgcaggt cacgcaaccc tctttgtaga tcagggtccc tttttcaccg
     1741 cattctgggc atttctcctt ggccaccttc gtaccgtcgg gaatgtattt tttcagcgcc
     1801 ctgactaccc cgttcttcca ggtgttgatc gattcggatt ccagttgcag gttatcgacc
     1861 aggtggacga caaacggcag cggcatacca tggcgtaata tccccgagat cagtttggcg
     1921 taattccaga attcggcatt gaactggatc gaaaggcctt cgagggtcac tttcatcccg
     1981 tatttgttct ggtactggaa atcgtaccgt gaattgtgct cgtcgagttt gtttttgatg
     2041 atctggcccg atttgaccca gtcggggacg acaaatcctt cggcctcgcc cgtgaagatt
     2101 tcataaggcc ggttgtcgag gataccgatt acggcaaccc atttctggtt gtcgttcata
     2161 aaatgcaaaa cttctccatc gaggacacgt ggccgtttcg gggcgctggt ctcgtggaac
     2221 tgcgcctctt ctttgttatc ctgctccttt ttcttttcgt ccttaatcaa aacgcccgcc
     2281 cgggatccgt cacggtaaat cgtcatccct ttgcagccgc tttcccacgc tgtcatatat
     2341 atctggctga ccaggtcctc ggtggtctct ttcggcacat tgaccgtcac gctgatgcta
     2401 tggtcgaccc acttctgaat agcgccctgc atcgtcacct tgctgaccca gtcgatatcg
     2461 ttgctggtcg cccggttata cggcgaaagt tcgatcagcc ggttcagctc atcttcgtga
     2521 tatgctttta cctcttcggt gtcgtacccg ttgattttca gccactcctc gaatttcgga
     2581 tggaatacgt tgtattcttc ccacgaatcg ccgacttcat caacaaaatc gatccggacg
     2641 tcgagatcgt tcgggtttac tttccttctg cggcggtaag aaacggagaa taccggttcg
     2701 atgccggagg aggtacgggt gcagatgctc acactaccgg tcggggcgat ggtgagcatg
     2761 gcaatatttc gccgcccgta ttttttcata tcctcataca gctcgggatc ggcttcgcgg
     2821 atacgattga tgaacggatt attcttttcc cgttcggtgc tgtagaccgg gaacgggccg
     2881 cggtcgcggg ccaggtcgac cgaggagcgg catgcctcca gggccagcat tttatgcact
     2941 tcggtagaga agctgatggc ttcttcggaa ccatagcgca gcccgagtgc ggccagcata
     3001 tcgccttcgg cggtgatgcc aattccggtg cgcctcccct ggatgcactt ctcgcggatg
     3061 ttcagcc
//
LOCUS       DAOQXS010000379         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34009_length_3065_cov_4.180731, whole genome shotgun sequence.
ACCESSION   DAOQXS010000379 DAOQXS010000000
VERSION     DAOQXS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34009_length_3065_cov_4.180731"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..2273
                     /gene="ppk1"
                     /locus_tag="PKM34_10005"
     CDS             165..2273
                     /gene="ppk1"
                     /locus_tag="PKM34_10005"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNQ83965.1"
                     /translation="MELNDQYFFNREISWLHFNERVLQEAIDPATPLLEQVKFLGIYS
                     NNRDEFFRVRVATLKRMIKLEQNETVPDLKYKVILDEVLEIVGGQEKLFIKAYNKVVR
                     ELARNDIYIINEKQVTPEQGEFITQFYKEKVRPNLFPLMLGNVRSSLSLQDASIYLTV
                     ILKKSAEPTVEDYALIQVPSDINGRFLVLPMDGRKSFIILLDDVIRYCLKDIFSAFGY
                     DRFESYTIKFTRDAELEIDNDVSKSFLELISVSVKKRKLGEAVRFVYDNTMPDDLLKI
                     LIKKLNIAKKDTIRGGGRYHNFKDFMDFPRIGDEKLRYPPAPPVPHRDLVNQPSMFKA
                     IRRKDIMLFFPYQSFQYIIDLLREASIDPDVRSIKMTFYRTTRNSAAMNALINAARNG
                     KYVTVFMEIQARFDEEANIYWTRRLQDEGIKVIQTIPGYKVHAKLILIRRRENGQNRL
                     YANVSTGNYNETTAKVFSDFSLLTCDPRICDDVYNIFELLESKFILPTFKNLIVSPFN
                     IRGFFIDMLDREIQNAKKGLEAWAIIKLNSLVDRRAAAKLYEAARAGVQINLINRGIC
                     VIKPGMPGVSLNINAFSIVDKYLEHSRVYIFCNGGYPEYFISSADWMPRNFDHRIEVV
                     CPIYDTGIQQELMDVLKIQMQDNTKSRYLGPEQLNRYRTTDDPVKHRAQFEIYDYYHK
                     KVKIKPHGQPGKSKSNCKEG"
     gene            2230..2697
                     /locus_tag="PKM34_10010"
     CDS             2230..2697
                     /locus_tag="PKM34_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83966.1"
                     /translation="MVNPENQNQTARRDKKIHIPSPARKIGYIVVIIILIVILYLIRN
                     YEKWNLTFLTKEFSKCLFYIELSIYINIGANILFIFYDNKWFKHLLQAITDVAGALSL
                     IMIYVIYPLNISNGDYDKWIRTGLLIIFVLTVISILINLIKGIRYLVREPEAV"
     gene            2800..>3065
                     /locus_tag="PKM34_10015"
     CDS             2800..>3065
                     /locus_tag="PKM34_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83967.1"
                     /translation="MRFELTQAQYFSLGTDAASVKWDRIKTENFSVIFPRQLDSQALR
                     TANTLEYFREMALEGRNIRTGRWPVILHNGTVIPNATTPFAPKR"
BASE COUNT          857 a          753 c          701 g          754 t
ORIGIN      
        1 cccctttgtt gcaagcataa caatatttgt ttgaataaaa aaacaaacat ataagttcca
       61 cgaaggactt ttaagccacc ctcttcatat ttcaactgac tatcaaagta aattcataaa
      121 tttgtgagat aaattcaaaa tgaaggaccg gataaaatca gcttatggaa ttgaacgatc
      181 agtatttttt taaccgtgag ataagctggc ttcatttcaa tgagcgggtt ctgcaggagg
      241 cgatcgatcc cgccactccc ctgcttgaac aggtgaaatt tctcgggatt tattccaaca
      301 accgcgatga gtttttcagg gtcagggtgg ctaccctgaa aaggatgatc aaactggagc
      361 aaaacgagac tgtacccgat ttaaagtaca aagttatcct ggatgaagtg ctggagatcg
      421 ttggcgggca ggagaagctg ttcattaaag cctataacaa ggtagtaagg gagctggcca
      481 ggaacgacat ttatattatc aatgaaaaac aggtcactcc ggaacagggt gaatttatca
      541 cgcagtttta taaagaaaag gtacgtccca accttttccc gctgatgctc ggcaatgtga
      601 ggagttcgct gtcattgcag gatgcttcga tctatctgac ggtcatttta aagaaatctg
      661 ccgagcccac ggttgaagat tatgcactga tacaggttcc cagcgatatc aacggccggt
      721 tcctggtcct gccgatggac ggccggaagt cgttcatcat cctgctggat gatgttatcc
      781 gttattgcct taaagacata ttctccgcct ttggatatga caggtttgaa tcctacacca
      841 tcaaatttac acgcgatgcg gaactcgaga tcgataacga cgtttcgaaa agtttcctgg
      901 aactgatcag tgtcagcgtg aaaaaaagga aactgggaga ggctgtgcgg ttcgtttacg
      961 ataataccat gccggatgat ctgctgaaaa tcctgatcaa gaagcttaac attgccaaga
     1021 aagacacgat ccgcggaggt ggaaggtacc acaatttcaa ggatttcatg gactttccga
     1081 ggattggcga cgaaaagctg aggtatccgc cggcgccacc ggtcccgcac cgcgacctgg
     1141 ttaaccagcc cagcatgttc aaggccatcc ggcggaaaga catcatgctg ttttttcctt
     1201 accagtcgtt ccagtatatc atcgatttgc tgcgggaagc atcgatcgat ccagatgtcc
     1261 gttcgatcaa aatgaccttt taccggacca cgcgcaactc tgcagccatg aatgccctga
     1321 tcaacgcggc acgcaacggg aaatacgtaa cggtttttat ggagatccag gcccggtttg
     1381 atgaggaggc aaatatctac tggacccgca ggctccagga cgaaggcatt aaggtaatcc
     1441 agaccattcc cggctacaag gtgcacgcca agctgatttt gatcaggcgc cgcgaaaacg
     1501 ggcaaaaccg gctctacgcc aacgtcagca caggcaatta caatgaaaca acagcaaaag
     1561 tattttccga tttcagcctc ctcacctgcg acccgagaat ttgcgatgat gtatacaata
     1621 ttttcgagct gcttgaaagc aaattcattc tgccgacatt caaaaacctg atagtatcgc
     1681 cattcaatat acgcgggttt ttcatcgata tgctcgaccg ggagatacag aatgcgaaga
     1741 agggtttgga ggcatgggcc ataatcaaac tgaacagcct ggtcgaccgc agggcagcag
     1801 ccaagctata cgaagccgcc cgggcaggcg tacagatcaa tttgattaac cggggcattt
     1861 gtgtcattaa gccgggaatg ccaggtgtca gcctgaatat caatgccttt tccattgtcg
     1921 ataaatacct cgagcacagc cgggtctata ttttctgcaa cggtggctac ccggagtatt
     1981 tcatctcttc ggccgactgg atgccgcgga atttcgacca ccgcatcgaa gtcgtttgcc
     2041 ctatttacga caccggcata cagcaggaac tgatggatgt tctgaaaatc cagatgcagg
     2101 ataacactaa atcgcgctac ctgggaccgg agcaactgaa ccgctaccgc accacggatg
     2161 atcctgtaaa acaccgtgcc cagtttgaaa tctacgacta ttatcataag aaagttaaaa
     2221 tcaagcccca tggtcaaccc ggaaaatcaa aatcaaactg caaggaggga taaaaagatc
     2281 catatccctt cgccggcccg gaaaatcggg tatatcgtcg tgatcatcat cctgatcgtc
     2341 atcctttatc ttatccgcaa ttatgaaaaa tggaacctga cgttcctcac gaaagaattc
     2401 agcaaatgcc tgttttatat tgaattatcc atatacatta acataggggc caatatcctt
     2461 tttatctttt acgataacaa atggttcaag cacctgctgc aggcgattac cgatgtagcc
     2521 ggcgccctct ccctgatcat gatctatgtc atctaccctt tgaacatcag caacggcgac
     2581 tacgataaat ggatcaggac gggattgctg atcatctttg tccttaccgt tatttccata
     2641 ctgatcaatc tgatcaaggg gatcaggtac ctggtgcggg agccggaagc cgtctgacaa
     2701 gcaataaaga attggaaagg tatgcagctc cccgtctccc ggtctccccg tcttcaaatc
     2761 tcctggcatt cctttcttct cctctttgta ttttgcgctt tgagatttga actaacccag
     2821 gcgcagtatt tttcactggg aaccgatgcc gcttcggtaa aatgggatcg gatcaaaacg
     2881 gaaaacttca gcgtcatctt tccccggcag ctcgattcac aggctctgcg caccgccaat
     2941 accctggaat acttccgcga aatggcactg gaaggtcgaa atatcaggac tggccgctgg
     3001 ccggtcatcc tgcataacgg aactgtcatt cccaatgcta ccaccccttt tgccccgaaa
     3061 aggat
//
LOCUS       DAOQXS010000380         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34068_length_3062_cov_3.088793, whole genome shotgun sequence.
ACCESSION   DAOQXS010000380 DAOQXS010000000
VERSION     DAOQXS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34068_length_3062_cov_3.088793"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1429
                     /locus_tag="PKM34_10020"
     CDS             272..1429
                     /locus_tag="PKM34_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ83968.1"
                     /translation="MGFISLIYVVFASIALFACVLQLIVTFKKENDLTFLVGAFLSLV
                     VFGEFLFLVLCTTRFGIECEPGLILRISLLFNIAVFICMMGVIAHLFKEKRRIIWLST
                     VALLLLIFLFVLSLPDEILFGSGTTIRKTWLTYGDRVILSGQGSSVWRILLSLSVLLF
                     LAFSGLIFFRHPFKPGRSSFLFILVGSGLIFITGTADALIDLEWVKFSYVMPLGIFSY
                     YLILIFIPFIRFIDEINNQQVFVREEKRYQHLASKAELIVVSLNRTGHVEFINPYFYR
                     LTGFKPEEVIGKDWFEFFIPPKEHYQMQGAFLELLHTENHPRHINPILTKDNKQIMIN
                     WFNVRTHDQSGTITGSLSIGVDISEHVEENRSLKQKLRDAEKLIEKLKAER"
     gene            complement(1434..>3062)
                     /locus_tag="PKM34_10025"
     CDS             complement(1434..>3062)
                     /locus_tag="PKM34_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNQ83969.1"
                     /translation="DLESYMDTLIGYIAAAQEPDGYLYTNRTIDPKNTHEMAGPERWI
                     NEEAHSHELYNAGHLYEAAVAHYQATGKRELIDVALRNADLIDNIFGWGKIEKAPGHQ
                     EIEIGLVKLFRTTGEVKYLDLAKFFLDVRGQAGEEYNQMHMKVKDQNEARGHAVRAQY
                     MYSAMADIAALTADTGYLEAIGRIWQDVTEAKTYITGGVGSVRQTEGFGPPFHLPNEE
                     AYCETCAAIANALWNYRMFLLHGDSKYYDVFEKVIYNGFLSGVSLGGDQFFYPNPLSC
                     EGQCRRKPWFGCACCPVNITRFLPSLPGYTYATYGSVLYINLFIGSAALINLADRRFT
                     IHQETEYPWEGRVTFRVETEQPIVFTMLIRIPGWAKNEAFPTGLYRFADTLAEKPELE
                     INGVKEEIILDRGYARIERKWSDRDSVVLALPYQPRKVIADQRVEAVRGKTALQCGPV
                     VYCLEDIDQPDSSIIQGKTQGSEVIIFGYDQYLLGGIGTLNGHIQTNDNQENNSSAVI
                     YEKFRAIPYYSWANRDQNKMDVWIGMNKDRKKQE"
BASE COUNT          764 a          718 c          651 g          929 t
ORIGIN      
        1 ttcactttcc gatatactgc tgattaaaat gggtccgttt ttatactcct tagcgagttt
       61 taccagggcc agaatagaat attgggcttt tttacttaac atctatcgta aaaaccaaaa
      121 tcccgccatt gaaggaatat ggcttattat taattagtta attaaataca gtaaaacaaa
      181 gtatacgatt gtaaaagtaa taaatacttg actaaagaca atcatttgtt taactttgtc
      241 acagttttgc caattcttcg tttatttggg aatgggtttc atctcgctca tatatgtggt
      301 ttttgcttcc attgcgctat ttgcgtgtgt ccttcagctt attgtcacat tcaagaaaga
      361 aaatgacctg acttttctcg tcggagcatt cctttccctg gttgtttttg gtgaattttt
      421 attcctggtt ctatgcacga cccgttttgg cattgaatgt gaaccgggtt tgattctcag
      481 gatttcactg cttttcaata ttgccgtttt tatatgtatg atgggggtta tagcgcatct
      541 gttcaaagaa aaacgaagaa tcatctggct ttcaaccgtt gcactcctgc ttttaatctt
      601 cctgtttgtc ctttcattgc cggatgagat ccttttcggc agtggcacta ccatccggaa
      661 aacatggctt acgtatggcg acagagtcat cctgtcaggc cagggtagtt cggtttggag
      721 gatcttactg agcctttctg tcttactttt ccttgctttt agcggcctta ttttctttcg
      781 ccatcccttt aaacccggca ggagttcatt tttgtttatc ctcgtaggca gcgggctgat
      841 ttttatcacc ggaacggcag atgcccttat cgaccttgaa tgggtgaaat tctcctatgt
      901 gatgccactc ggtatatttt cttattatct tattctgatt tttattccat tcataagatt
      961 tatagacgag atcaataacc agcaggtctt tgtcagggaa gagaagagat atcagcatct
     1021 tgccagtaag gctgaattga tagtcgtttc cttaaatcgt acagggcatg tggaatttat
     1081 caacccatat ttttacagac tgacaggttt taaaccggaa gaggtaatcg gcaaagactg
     1141 gtttgagttt tttattcctc ctaaagaaca ttaccaaatg cagggggcat ttttagagct
     1201 gcttcacaca gaaaatcacc cacggcatat taatccgatt ctgacgaaag ataataagca
     1261 gatcatgatc aattggttta atgtcagaac ccatgaccaa tcggggacca ttacaggtag
     1321 cctgagtata ggtgttgata tctctgaaca tgttgaggaa aaccggtcgc tgaaacagaa
     1381 actccgggat gccgaaaagc tgattgagaa attaaaagcc gaacgataac aggctattcc
     1441 tgctttttcc tgtccttgtt cattccaatc cagacatcca ttttgttttg atccctgttt
     1501 gcccaactgt aatagggaat ggccctgaac ttctcgtaaa taacagcact tgagttattc
     1561 tcctggttat cattagtttg gatatgaccg tttaacgttc cgatcccgcc gagcaggtac
     1621 tgatcgtacc cgaatataat gacttctgat ccttgagttt taccttgtat tatcgatgaa
     1681 tctggctggt cgatatcctc caggcaataa actaccggcc cgcattgaag cgctgttttc
     1741 ccccggacgg cttcaacccg ctggtctgca ataaccttgc gtggctgata ggggagtgct
     1801 aaaacgacag aatcccggtc cgaccatttc ctttcgatac gtgcatatcc ccgatccagg
     1861 attatttctt ctttcactcc gttgatctcc agctccggct tttctgccag tgtgtcggca
     1921 aaccgataaa gcccggtggg gaacgcttca ttttttgccc atccgggaat cctgatcagc
     1981 atggtgaaaa ctatgggttg ctcggtttca acccggaatg tcacgcggcc ctcccatgga
     2041 tattccgttt cctggtggat tgtaaatcga cgatcagcca gatttatcaa agcggcgctg
     2101 ccgataaata aattgatata taatacactg ccatatgttg catatgtgta acccggcagt
     2161 gatggcagga accgggtaat gttgaccggg cagcaggcac agccgaacca gggttttcgc
     2221 cggcactggc cttcacacga aagcgggttc ggataaaaaa attggtcgcc gccgagtgaa
     2281 actccggaca gaaagccatt gtatatcact ttctcgaaaa catcgtaata ttttgaatca
     2341 ccatgcagga gaaacatccg gtagttccat aatgcattgg ctatagcagc gcatgtttca
     2401 caataagctt cctcgttggg aagatggaac ggagggccga atccttcggt ctgcctgacc
     2461 gaacccacac caccggtaat ataagtcttg gcctcggtaa catcctgcca tatccggcca
     2521 atagcttcca aataaccggt atcggctgtc agggcagcaa tatcagccat cgccgaatac
     2581 atatactgcg cccgcactgc atgtcccctg gcttcattct gatctttcac tttcatatgc
     2641 atctggttgt attcctctcc tgcttgtccc ctgacatcca gaaaaaactt cgccagatca
     2701 aggtatttca cttctccggt cgtacggaac aatttcacaa gcccgatctc tatctcctgg
     2761 tgaccgggcg ctttttctat cttaccccaa ccgaaaatat tatcgattaa atcagcattt
     2821 cgcaatgcga catcaatcaa ttccctttta ccggtggcct ggtaatgggc gacggcggcc
     2881 tcataaaggt ggccggcgtt gtaaagctcg tggctgtggg cctcttcgtt gatccacctt
     2941 tccggcccgg ccatttcatg cgtatttttc ggatcgatcg tccggttggt gtaaagatac
     3001 ccatcaggct cctgggctgc agctatataa ccaatcaggg tatccatata ggattctaaa
     3061 tc
//
LOCUS       DAOQXS010000381         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34098_length_3060_cov_3.820965, whole genome shotgun sequence.
ACCESSION   DAOQXS010000381 DAOQXS010000000
VERSION     DAOQXS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34098_length_3060_cov_3.820965"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1198
                     /locus_tag="PKM34_10030"
     CDS             155..1198
                     /locus_tag="PKM34_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83970.1"
                     /translation="MSYTRHYRETVSTTVSISYPKSENGGTVSKTVNIPVDVNIHVDT
                     QPFDASVHQVNTSVDLLTGAVVATEAAEIISREKNSKKVANTILDGFFSYIRSEISQQ
                     VAELSQSIDAHFMHLQELMHSCLKKKAQMEGDYQRISSRYTKVFEDLNQELSNRIYEL
                     DKPAFMFKRETDSHKIRSVENDLVSTIAIFGFESSELQTRISASVAKKRAVDTLMQAR
                     LFLMQQKRLNYTIQHSMLNEEYSSTVYIPVCYMETRNDKEQIDQKIYYSSGIPVFNQD
                     KMKNDMVSRFASKTVTWTKVDNDTSGNLGLYFTSVLNAGIQSNDMHSSRVIEMIRRIA
                     DIDKIYVIKISNQ"
     gene            1203..2177
                     /locus_tag="PKM34_10035"
     CDS             1203..2177
                     /locus_tag="PKM34_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83971.1"
                     /translation="MKELKELRFNETNIQLKDNLVKGSILPEKIAELNRTITIQGNSV
                     IEGAVYAYKLEVQNGDSDIQGAVFTQHEMYVNSEAKGNISFKKSVGSANSIVSRAQKC
                     NLNFYSDINAKSVTLYNAFVAGSIYADEIILQNCVVIGGVFATQSIDLTNSIIGTFNT
                     PSVKASQTVWLLLPSAFSIERINVIPGTRFYNISLADLGSLYKEMKESENSGKIEMNL
                     EADEVKTTLSSHDTQKTLRSYTVIGKVLAADLLDTDKFQNHFLLTAASLGSQLLKTYD
                     LGMNDKGEPIMLTFEKIRDFFFNILNGKIEIQNIEGKFNISDLTGKFN"
     gene            complement(2242..2991)
                     /locus_tag="PKM34_10040"
     CDS             complement(2242..2991)
                     /locus_tag="PKM34_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNQ83972.1"
                     /translation="MIREFNILRSHGIQYVPDLKKAELTPLFRGRPVISPSVTDDEKT
                     MLRKLCPSGAILENDFGIDLGKCVFCKECAFACPGKISFTNDYKIASNNRNGLIIRPG
                     NESVGFDETRIRKEVRKLFRNSLKLRQISAGGDNSCEMELNASGNVNFDMGRYGIEFV
                     ASPRHADGIVITGPITENMAAATQICYDAVPHPKIIILVGTDAISGGLFSESKAINRS
                     FLDKYPVDLYVPGNPAHPLTFINGLLKLQGK"
BASE COUNT          951 a          670 c          648 g          791 t
ORIGIN      
        1 attgcactgg tcagctaaag aagccccttc agccgaggtc agaagcgaat tcagcaaatt
       61 tatctccact tctggtaaac ctcagcgcat taaagacatg gctatgaaac tgtaccagtc
      121 atcctctttt caaaccctca aataacttac gcatatgagt tataccaggc attacaggga
      181 gaccgtttct acaaccgtct caatctcata tcctaaatcc gaaaacgggg gaacagtttc
      241 aaaaacggtc aatataccgg tagacgtcaa catacatgtc gatacccaac catttgatgc
      301 aagcgtgcac caggtgaata cgagtgttga ccttttgaca ggggcggtag tcgctacaga
      361 ggccgcagag atcatatcgc gcgagaagaa cagtaaaaaa gtagccaaca ccatactgga
      421 tgggttcttc agctatatcc ggtcggaaat cagccaacag gtggccgaat tgtctcaaag
      481 catagatgcc cattttatgc atctccagga attgatgcat agctgtctga agaaaaaagc
      541 acagatggaa ggtgattatc agcgaatatc aagccggtat acaaaagtct ttgaagacct
      601 gaaccaggaa ctatcgaaca ggatttatga actcgataaa cccgctttta tgtttaagcg
      661 ggaaacggac agccataaaa tcaggtccgt agagaacgat ctggtgagta ccattgctat
      721 tttcggattt gaaagcagtg agctccaaac ccggatcagc gcttctgtcg ccaaaaaaag
      781 agccgtggat accctaatgc aggcccggct gtttttaatg caacagaaaa gactgaacta
      841 cactatacag cactctatgc tgaatgagga atacagctca accgtctata taccggtttg
      901 ttatatggaa acccggaatg ataaagaaca gatcgatcag aagatctatt attcatccgg
      961 gattccagtc tttaaccagg ataaaatgaa aaacgacatg gtatcccggt ttgcatcaaa
     1021 aacggttacg tggacaaagg tcgataatga tacctcagga aacctcggac tctattttac
     1081 ttctgtattg aatgccggaa tacagtcaaa tgacatgcat tcctcccggg taatagagat
     1141 gattagaaga attgccgata tcgataagat ttatgtcata aaaatttcta accaataaaa
     1201 taatgaaaga attaaaagaa ttaaggttta atgaaaccaa tatacaatta aaggataacc
     1261 tggtaaaggg ttctatccta cctgaaaaga tagccgaact gaaccggacg ataactatcc
     1321 agggaaattc tgtcatagag ggagctgttt atgcctataa acttgaggtt cagaatggtg
     1381 attcagacat acagggtgcc gttttcacac agcacgagat gtatgtgaac agtgaagcta
     1441 aaggcaacat atcgtttaaa aagagtgtcg gttccgccaa ttcaatcgtt tcccgcgctc
     1501 agaaatgcaa cctgaacttt tattctgaca tcaatgccaa gagtgttaca ctttacaatg
     1561 cttttgtggc cggaagcatt tatgccgatg agatcatatt gcagaattgt gtcgtcatcg
     1621 gcggagtttt tgccacccaa agtatcgacc tgacaaattc tataatcgga acattcaata
     1681 cgccatcagt caaggcatcc cagactgtat ggctcctgct tcccagcgca ttctccattg
     1741 aaaggatcaa tgttatcccc ggaaccagat tctacaatat cagcctggca gatttgggtt
     1801 ctctttataa agaaatgaaa gaatcggaaa attcagggaa gatagagatg aaccttgaag
     1861 cagatgaagt gaagacaaca ttgagcagtc acgataccca gaaaacactc cggagctata
     1921 cagtcatcgg aaaagtactg gcggcggacc tgcttgatac ggacaaattc cagaaccatt
     1981 ttcttttaac agctgccagc cttggatcgc agcttctaaa gacttatgac ctgggaatga
     2041 atgataaagg agagcccatc atgcttacat ttgaaaagat aagggatttc tttttcaata
     2101 ttctgaatgg aaagattgaa atacagaata tagagggtaa attcaacatt tcagacctta
     2161 caggcaagtt caattgaaac gggactgaaa aatttcgtgc aattttttat tgcccggaat
     2221 tagaccaaat tgagatacaa actattttcc ttgcaatttc aaaagtccat tgataaatgt
     2281 aagcggatgg gccggattgc cgggtacata gagatcaaca ggatatttgt ccagaaatga
     2341 gcggtttatt gctttgcttt cagagaaaag ccccccactg atggcatctg tacccaccag
     2401 gattataatt ttgggatgag gcaccgcatc gtaacagatt tgtgtagctg ctgccatgtt
     2461 ttcggttatc gggccggtta tcacgatacc atccgcatgc cggggcgatg caacaaattc
     2521 gatcccatac cgccccatgt cgaaattgac attaccggaa gcattcagct ccatctcaca
     2581 actattgtcc cctccggctg agatctgcct cagttttagc gaattcctga acaatttccg
     2641 gacttctttc ctgatccgag tctcgtcgaa gcccacactt tcattccccg gcctgataat
     2701 cagtccatta cggttgttcg aggctatttt ataatcgttg gtaaagctga tctttcccgg
     2761 gcaagcgaaa gcacattctt tgcagaaaac acatttcccg aggtcaatac cgaagtcatt
     2821 ctccaaaatg gcgccggaag ggcatagttt cctgagcatc gttttttcat catcggtgac
     2881 cgaagggctg atgaccggcc ttccccggaa caatggcgtc agttctgctt ttttaaggtc
     2941 aggtacatac tgaatgccgt gactgcgcaa tatattaaac tctctgatca tctgttataa
     3001 atcatatccg caatagctca ggttaaaact cttgttgcaa accgggaaat cggatatttc
//
LOCUS       DAOQXS010000382         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34123_length_3059_cov_3.575899, whole genome shotgun sequence.
ACCESSION   DAOQXS010000382 DAOQXS010000000
VERSION     DAOQXS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34123_length_3059_cov_3.575899"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKM34_10045"
     CDS             complement(<1..716)
                     /locus_tag="PKM34_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNQ83973.1"
                     /translation="MKKLLLLILIFSGCYAFSQDTFSIVAVDTVTGEIGSAGASCIDE
                     SQIEGGALIISDVMAGRGAIHTQSYWNATNQQNAHNRMVEGMSPQEIIDWLTTHDAQG
                     NPAVRQYGVVDFDEEGHARAAAFTGNNCMNYKNHIVGPNYAIQGNILLGQQILDSIEA
                     RFLNTQGSFAERMMSALQGANVVGADTRCTNNGTSSLSAFIRIARPQDTTGTFYCDLN
                     VPSVPDGMEPIDSLQVIFDE"
     gene            complement(925..>3059)
                     /locus_tag="PKM34_10050"
     CDS             complement(925..>3059)
                     /locus_tag="PKM34_10050"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ83974.1"
                     /translation="TDDNGYYEDTAEIPGYNGYGFVTAETYDSCLGYTVSQGQFIAPG
                     TTLMPMDFFLCTVINPECQAYFYYYQSNPVDPYTFTFENMSMGEYTEFFWDFGDSTYS
                     YEMYPQHTFPGPGYYNVCLTIGGTSECNDTYCESIYVGGGPSGCENSFYYYNENDPFT
                     LTFEGFLMNGQYAQYYSWDFGDGTTGSGQTVTHTYDPMGNVNLYMVSLTTIVMDSMGS
                     DSCFYTSYQEVWISNSTGCESFIIPMNMSGLTVEFEGYTVSPYETQYTWEFGDGVTGT
                     GQIVSHTYPSPGMYTVMLNTIDANGCAYDSFTQIWLDSTNNTGCSAYFMYDQTDSTTF
                     TFNGVIYLNNGTIYPDSSTTYSWDFGDGTFGSGQTVTHYFQENPVGGYTVCLTATMIM
                     NDGTACTSTYCEYINLVTPAFNIFGYVYLGNYFADQATVHLMTMDTLWQGVVEVQSTT
                     IDSGGYYSFPDIPLENYRVYYVQAELNEGSAYFGEYLPTYHLCALSWEEAFPVMPIMN
                     WPADIVMIAATEAATGSGMISGVVTDLGTRGNMENVEVVLMDSEKNPIRYMRSDDQGM
                     FVFDNLGLSTYIIHAEIMGIHTVQAAVTLTEQNPSTSVEVQVSGDEANIVFGIPDHPV
                     SFTNVGEIYPNPVKDAAAMDITLSKPALVTVRIVSVTGQLMDLRTYDLGKGTQRIELE
                     ALSFANGFYMMQITSDSGDSVIKKFLKQ"
BASE COUNT          789 a          755 c          735 g          780 t
ORIGIN      
        1 cattcgtcga aaataacctg gagcgagtcg atcggttcca tcccgtcggg taccgaaggg
       61 acattcagat cgcagtaaaa agtgccggta gtgtcctgcg gacgggcgat acggataaat
      121 gcagagagcg atgaggtgcc gttgttggtg caccgggtgt cggctccaac aacgtttgct
      181 ccctgcaggg ccgacatcat tcgctctgca aaacttccct gcgtattaag aaaccgtgcc
      241 tcaatactgt cgaggatctg ctgtcccagg aggatatttc cctgtatggc ataattcggt
      301 ccaactatat gatttttgta attcatgcag ttgttcccgg tgaatgcggc agcacgtgca
      361 tgcccctctt catcgaaatc gacgacacca tactgcctga cggccggatt cccctgcgca
      421 tcatgtgttg tgagccagtc gatgatctcc tgggggctca tcccttccac catccggttg
      481 tgggcattct gctggttggt ggcattccag tacgactggg tatggatagc tccgcgtccg
      541 gccatcacat cgctgatgat cagggcgccc ccttcgatct ggctctcgtc gatacaggat
      601 gcgccggcgc tgccgatctc acccgtcacc gtatctacgg ctacgatcga gaaagtgtcc
      661 tgggaaaaag cataacagcc cgaaaatatc agaatgagaa gcagtagttt tttcataata
      721 atgaagatta tcttatcaaa aataataaaa aaaagaggct gtctcaaaag cattaaacca
      781 caaaggaaca ccaaggaatt cacaaaggcg cacaaaggta tgttaattga tattcaacta
      841 cttagtgttc ctttgtgatg tactttgtgc gccttagtgg ttaataaaaa accttttgag
      901 acagcctctc cgagtatcta ttacttactg tttaaggaat ttcttgatta ccgagtcccc
      961 gctgtcactg gtaatttgca tcatataaaa gccattggcg aaggaaagag cctccagttc
     1021 gatccgctgt gtgcctttgc caagatcata ggtccgcaag tccatcagct gaccggttac
     1081 cgaaactatc ctgaccgtta ccaaagcagg cttggagagt gtgatatcca tagcagccgc
     1141 atccttgacc ggattcgggt atatttcgcc aacgttcgtg aaagagaccg gatgatccgg
     1201 gataccgaag acgatattgg cttcatcgcc gctgacctgc acctccacgc tggtcgaagg
     1261 attctgttct gtaagggtta ccgcggcctg cacggtgtgt attcccatga tctcggcatg
     1321 gatgatatag gtgcttaatc ccaggttatc aaaaacaaac attccctggt cgtcgctccg
     1381 catataacgg atcgggtttt tctctgaatc catcagcacc acctccacat tttccatatt
     1441 tccccttgtg cccaggtcgg taacaacacc ggagatcata ccgctgccgg tggctgcttc
     1501 ggtggccgcg atcataacaa tatcggcagg ccagttcata atgggcatca cgggaaatgc
     1561 ctcctcccag cttagcgcac aaaggtggta agtcgggaga tattccccga agtatgccga
     1621 tccttcgtta agctcggcct ggacataata tacacggtag ttttcaagcg gaatatcggg
     1681 gaagctgtaa taaccgcccg aatcgattgt ggtggattga acctcgacca cgccctgcca
     1741 cagggtatcc atggtcatca ggtgaacggt ggcctggtcg gcaaagtagt tgccgagata
     1801 aacatatccg aaaatattga atgcaggggt gacaagattg atatattcac aatatgttga
     1861 agtgcaagca gttccatcgt tcatgatcat cgtcgcggtc aggcatacgg tataacctcc
     1921 aaccggattt tcctggaaat aatgtgttac tgtttgcccg cttccgaagg ttccgtcgcc
     1981 aaaatcccag gagtaggttg tggaagaatc ggggtaaatg gtcccattat tcagatagat
     2041 aacaccgttg aaggtaaacg tcgtgctgtc ggtctgatca tacataaaat aagcgctgca
     2101 tcctgtatta ttggtcgaat caagccatat ctgcgtaaaa ctgtcatagg cgcagccgtt
     2161 ggcatcgatg gtgttgagca tcacggtata cataccgggc gacggatacg tgtgcgaaac
     2221 gatctgtcct gtccccgtca ccccgtctcc gaattcccag gtgtactgcg tttcataagg
     2281 actgacggtg taaccctcaa actcaacagt aagcccgctc atgttcatcg gaatgataaa
     2341 gctctcgcat cccgtggaat tgcttatcca gacctcctgg taagaagtgt aaaaacaact
     2401 gtcggagccc atcgaatcca tgacaatggt tgtcagtgaa accatgtaca ggttcacatt
     2461 tcccataggg tcataggtat gggtgacggt ctgtccgctg cccgtagtgc cgtccccgaa
     2521 atcccagcta taatactgcg cgtattggcc gttcatcagg aatccttcaa atgtcagcgt
     2581 aaacggatca ttctcattat aataatagaa tgaattttca caaccggagg ggcctccgcc
     2641 aacatatata gattcacagt aggtgtcgtt gcattcgcta gtcccgccaa tcgtcaggca
     2701 tacattataa tatccaggtc ccggaaaagt atgttgcgga tacatttcat aagaataggt
     2761 gctgtcgccg aaatcccaga agaattcggt gtattctccc atcgacatgt tttcgaatgt
     2821 gaatgtgtaa ggatcgacgg ggttactctg ataataatag aaataagcct ggcattccgg
     2881 gttaataacc gtgcagagga aaaaatccat tggcatcaat gttgtccccg gggcaatgaa
     2941 ttgcccctgc gaaacagtat atccaaggca tgagtcatag gtttcggccg taacaaatcc
     3001 atacccgttg taacccggta tttctgcagt atcttcatag taaccgttgt catcggtat
//
LOCUS       DAOQXS010000383         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34610_length_3029_cov_3.117687, whole genome shotgun sequence.
ACCESSION   DAOQXS010000383 DAOQXS010000000
VERSION     DAOQXS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34610_length_3029_cov_3.117687"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKM34_10055"
     CDS             <1..417
                     /locus_tag="PKM34_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83975.1"
                     /translation="LIINNQGFLWGEHGPGARSVYKTLAESGFKVKYYFAGDDKNSGD
                     IHFIASPADFRLPMEIPQNINACCREYPVDYPALVSAIEFGSAANIPDLRDAHILTDD
                     KPLLLTLYNYSNEEWRQNTMKSLLRGQSPPPVTFFH"
     gene            438..2135
                     /locus_tag="PKM34_10060"
     CDS             438..2135
                     /locus_tag="PKM34_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNQ83976.1"
                     /translation="MHKHPGISDKWIRASVIGTTWAASEIVLGSFLHNLRIPFSSNIL
                     AGIGIIALVSVSYKWQQKGLFWRAGLICALMKTMSPSAVIFGPMIAIFSQALMLEIFT
                     RVLGRNFFGFAIGAMFATTWNLFQKIINFIIFYGFDIVDVYSGLLSYAQKQLNIRLDL
                     VWSPVILLAIIYSSLGLVSAIIGMRIGRKLLSQEPVAVKAGNNANLSFLKPKTQSFPY
                     SIGWLFLDMALIITAFILLNFGSWMVWGSGIIIITTIWVIRYKRALRQLARPTFWLYF
                     VLITMGTAFVINRVQSREFVEGLIIGIQMNFRAALVILGFSVLGTELYNPRIREFFLR
                     TSFKQLPMALELSFDSLPIMISAFPDIKEIVKSPVSVIYRVISQIDFRLEEIRRKLQR
                     KVFIISGPVGGGKTTQIRKIIEELWKKNIQVDGIYSPRVMEDGLTAGYDVVDVETGQR
                     EPFLRKAADSAHAAVGQYDIHPAGLELGLAALTRSHEKQCRLVVIDEVGRLEVNDRGW
                     SEKLALLIGQAQVLVLTVRDTYSDEVIKKWGLKDYEVFTATGDLYVKITDKIAGLTG"
     gene            complement(2233..>3029)
                     /locus_tag="PKM34_10065"
     CDS             complement(2233..>3029)
                     /locus_tag="PKM34_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83977.1"
                     /translation="EMKKIYVGDKLVWEKQTEIPVNYFNIYRETSQPGEYEVIGTVDY
                     EDSCVFADTTANPQEQPYKYRLSAFSLQGTESLPGNLHSTVHLSVNSVPGGCELEWTP
                     YEGFVFDKYYIYRGNEADNLTLLDSAAGGVNSFTDADPPFGPLFYAIEIILDGGCFPG
                     GDEIYHTSRSNAIFAGNIGIPEDAGSTFRIFPNPVHDILNIQAGTGMIRQDAEVSIYS
                     VYGIELISCQLTTGRTAIDIRELAPGIYFINLKSRNQIKTGKFVVQ"
BASE COUNT          717 a          736 c          797 g          779 t
ORIGIN      
        1 ctgatcatca acaaccaggg gtttttatgg ggagagcacg ggcccggcgc ccggtcggtt
       61 tacaagacgc tggcggaatc gggcttcaag gtgaagtatt atttcgccgg cgacgataag
      121 aattcaggcg atattcactt cattgcctct ccggccgatt ttcgcctgcc catggagatc
      181 ccgcaaaaca tcaatgcctg ctgcagggaa tacccggtgg attacccggc cctggtgagc
      241 gccattgaat tcgggtcggc tgcgaacatc cccgatttgc gcgatgcgca tatcctgacc
      301 gatgataaac cgctgctgct caccctgtac aattattcca acgaggaatg gcggcagaat
      361 accatgaaaa gcctgctgcg gggccagagt ccgccgccgg tgacgttttt tcactaatgc
      421 ggaaaaacct gtcgtgaatg cataaacatc cggggatcag cgacaaatgg atcagggctt
      481 ccgttatcgg caccacatgg gccgcctcgg agatcgtcct gggaagtttc ctgcacaatc
      541 tccgcattcc tttcagttcg aatatcctgg ccgggattgg gattattgcc ctggtatcgg
      601 tgagttacaa atggcagcag aaagggttgt tctggcgggc cgggctgatc tgtgccctga
      661 tgaagaccat gtcgcccagc gcggtcattt tcggcccgat gatcgctatt ttcagccagg
      721 ccctgatgct ggagatcttt acccgcgtgc tgggcaggaa tttcttcgga tttgccatag
      781 gggccatgtt cgccactaca tggaatctct tccagaaaat cataaacttc attatttttt
      841 acggttttga tattgtggat gtgtattccg gcttgctgag ctatgcgcag aaacaactga
      901 atatccggct cgacctggtc tggtcgccgg tcattctcct ggccatcatt tacagcagcc
      961 tgggccttgt ttctgctatc atcgggatgc ggatcgggag aaaactgctg tcgcaggaac
     1021 ctgtggccgt taaggccggc aacaatgcca atttatcctt cctgaaaccc aaaacacaat
     1081 cttttcctta ttccatcggc tggttgtttc tcgatatggc cctgatcatc accgccttca
     1141 tcctgctgaa cttcgggtca tggatggttt ggggatcagg tataatcatc attacaacga
     1201 tctgggtcat tcgttataaa agggcgctca ggcagctggc ccgccccact ttctggctct
     1261 atttcgttct gataaccatg ggtacggcgt ttgtcattaa ccgggtccag tcacgggagt
     1321 tcgtcgaggg gctgatcatc ggcatccaga tgaatttccg ggcggccctt gtcatcctgg
     1381 gattctccgt actgggcacc gagctttaca atccccggat cagggagttt ttcctgagga
     1441 cctctttcaa acagcttccg atggccctgg agctctcctt cgacagcctg ccgatcatga
     1501 tttctgcctt tccggatata aaggaaattg taaaaagccc ggtatcggtc atttaccggg
     1561 tgatctcaca aatagatttc aggctggagg agatcaggag aaaactgcag cgaaaggttt
     1621 ttataatctc cggaccggtt ggggggggaa agacaacaca gatccggaaa ataattgagg
     1681 aactctggaa aaagaacatt caggtcgatg gtatttattc tccgagggta atggaagacg
     1741 gcctgactgc gggttatgat gtggtggatg tggagaccgg ccagcgggag ccgtttttga
     1801 gaaaggcggc ggatagtgcg cacgcggccg tcgggcaata cgatatccat ccggccggac
     1861 tggagctggg cctggcggcg ctgacccgct cgcacgaaaa gcaatgcagg ctcgtcgtta
     1921 tcgacgaagt cggacgcctg gaggtgaatg acaggggatg gtcggagaag cttgctttgc
     1981 tgatcggcca ggcccaagta ctggttttaa ccgtcaggga tacttattcc gatgaggtaa
     2041 taaagaagtg gggattaaag gattacgaag tttttaccgc gactggcgat ctttatgtta
     2101 agattaccga taagatagcc ggtttgaccg gttgatattc agggatatta attagatagt
     2161 atttcatttc aaaaccgtat tgctttgctt ctgaaataag ttgaccaaca acctaaagtg
     2221 aagaaggcag catcactgca ccacgaattt ccctgttttt atctggttcc gggatttcag
     2281 atttatgaaa tagataccgg gggccagctc ccggatgtcg atggcagttc ttccggtcgt
     2341 taactggcag gagatcagtt cgataccata tacgctataa atggatacct ctgcatcctg
     2401 ccggatcatt ccggttccgg cctggatatt caggatatca tgaacagggt ttggaaagat
     2461 ccggaatgtt gatccggcat cttccggaat accgatattt ccggcaaata tcgcattgga
     2521 gcgggaggtg tggtaaattt catccccgcc agggaaacaa ccaccatcaa gaataatctc
     2581 aattgcatag aacaggggcc cgaaaggcgg atcggcatcg gtaaaggaat tgactccacc
     2641 tgcggccgaa tcgagcaaag tcaggttgtc cgcttcgttc ccgcgataaa tgtaatattt
     2701 gtcaaataca aatccttcat agggcgtcca ttccagttca cagccaccgg ggactgaatt
     2761 aacggataaa tgaaccgtgc tatggagatt tcccggcaat gattccgttc cctggagtga
     2821 aaaggccgat aacctgtatt tataaggctg ctcctgcgga ttcgcggtag tatcggcaaa
     2881 cacgcatgaa tcctcataat cgacggtccc gatcacttca tattctccgg gttgcgacgt
     2941 ttcccggtaa atattgaagt aattcacggg aatttccgtt tgtttttccc aaaccaattt
     3001 atcccccacg tatatttttt tcatttcgg
//
LOCUS       DAOQXS010000384         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34675_length_3025_cov_3.425926, whole genome shotgun sequence.
ACCESSION   DAOQXS010000384 DAOQXS010000000
VERSION     DAOQXS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34675_length_3025_cov_3.425926"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..781
                     /locus_tag="PKM34_10070"
     CDS             143..781
                     /locus_tag="PKM34_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83978.1"
                     /translation="MFTTTRENSRDHQSAMSRTICTFLLRISISVLLLSDHSLTIGQP
                     GIIKMPDNAVIVDNGRVGLILPPRSRESDKAFTEALKELKPSLLLIYDADTLLKDTPQ
                     IFLSKSVADTIIPMEIVFIENCVKVLPELDGKRSTLLDFGVFDIDGRRYRYKTIQPPD
                     PSDGHIKMMFYFMKDDYSNSLYELGIIFKKEYFDFVEPMIISIAASFEFLEP"
     gene            1194..1814
                     /locus_tag="PKM34_10075"
     CDS             1194..1814
                     /locus_tag="PKM34_10075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ83979.1"
                     /translation="MAIQDWNERYVGGDLPWDITEPDDNLVKLVGSGIVPPGKALEIG
                     CGTGTNALWLASKGFSVLGVDIAPEAIEMAHRKKNGQELSCDFAVMDFLEEGNLIKKY
                     DFVFDRGCFHSFDKESDRKLFAANVSRSLSPGGIWASLIGSTEGAARETGPPRRTASE
                     VIASVEPYLEIVELKSVLFRTNFPNAPKAWLCVSRQRKEPAQPSGR"
     gene            complement(1937..>3025)
                     /locus_tag="PKM34_10080"
     CDS             complement(1937..>3025)
                     /locus_tag="PKM34_10080"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNQ83980.1"
                     /translation="YFTDVENGWMVGNNGTAVGTADEGATWQTLGFGDGTLFDVTFTD
                     ADHGWIVGSAGTILHTVNGGSDWDYQASPTTGTLYDVCFVNNLRGWIGCDNGILLHTF
                     DGGLTWTEQDCGIEDYSDIKSVCFTDENNGWIALYSIPCELLHTNDGGQTWISQYTDN
                     SARFNSIFFTDSLNGWAAGNKVLHTGNGGLTWEVQLPLSVYQYYEVMFIDPNTGWIGP
                     PLRYTLDGGLTWKSYTCLEGPSDLWSFYFLDELNGWGAWADAVFHTTTGGFVDLPERE
                     RITSIKHFCYPNPFPEKIEICFNVGRPEKTGIQILDMMGKQVYRLIYDSTEGENIISL
                     DLNALSKGLYVYRIATKEKSVTGKIIKL"
BASE COUNT          831 a          680 c          685 g          829 t
ORIGIN      
        1 tgtccaactg tccaactgcc caactgtcca actgcccaac tgcccaactg cccggctgcc
       61 cggctgcctt aattatcccg ataaatcaga cctggagaat aactggaaaa gcttatcttt
      121 attcatcaat taaataatta ttatgtttac aacgacccgt gaaaacagta gagatcatca
      181 atctgccatg agccggacga tatgcacttt ccttctcaga atctctattt ctgtcctttt
      241 attgtcagat cattctctga caattggtca acctggtatt attaaaatgc ccgataatgc
      301 tgtgatcgtc gataatggaa gagtaggatt gatcctgcct ccgcgatccc gcgaatcaga
      361 taaagcattc acagaggcat taaaagagct taagccttca ttattattga tatatgacgc
      421 tgatacactg ctgaaagaca ccccacagat ttttctaagc aaatctgtcg cggacacaat
      481 tatcccgatg gagattgtct ttatcgagaa ttgtgtcaaa gtacttcctg agcttgacgg
      541 aaagcgctca actcttctcg attttggggt cttcgacatt gatgggcgca gatacaggta
      601 taagacaatc cagccgccag atccttcaga tgggcatatt aagatgatgt tttactttat
      661 gaaagatgat tattctaaca gtctttacga attgggaatt attttcaaga aggaatattt
      721 tgattttgtt gaacctatga ttatcagtat agctgcaagt tttgaatttc tcgaaccctg
      781 atgggagaac tttctttggt gttataacga ttcttattgt gagacatgca cgaaggcgaa
      841 ggtagttcct ttcgaaatcg tgcatagcgc atcggaaata agaaatcaat ccctgccctg
      901 gcataataaa aaaatacctt attggcagaa tttaatgctt aatgcttaat gtttaattag
      961 taatgagtaa tgtaataatg agtaatgttt taattagtaa tggggtaatt agtgatggct
     1021 aatggctaat ggctaacgac taacgaccaa ccctccttcg tctccgcaag ccggcgggtc
     1081 ctcggattgg accgtcgatg aacctttcgg gatttatttt gttttttaac ctatctttca
     1141 atatcctttc gggtgatgaa tggattacat tttaaagagg aggagcaatt tctatggcaa
     1201 tacaggactg gaatgagcgg tatgttggcg gcgatctgcc gtgggacatt accgaaccgg
     1261 atgataatct agtgaaactg gtcggatcgg ggatcgttcc gccgggcaaa gccctggaga
     1321 tcggatgcgg aacgggcacg aatgcgttat ggctggccag caagggattt agcgtgctgg
     1381 gcgtcgatat tgctcctgaa gccatcgaaa tggcccaccg caaaaagaac ggccaggagc
     1441 tgtcatgcga ctttgctgtg atggattttc ttgaggaggg gaatttaata aagaagtatg
     1501 atttcgtgtt tgaccggggg tgctttcaca gttttgacaa ggaaagcgac cgcaagcttt
     1561 ttgccgccaa tgtgagccgt tcactgtcgc cgggtgggat ctgggccagc ctgatcggca
     1621 gcacggaagg cgctgcaagg gaaaccggac caccacgccg gacagccagt gaggttatcg
     1681 cttctgttga gccgtacctt gagattgtag aattaaaatc agtgctcttc cggacaaact
     1741 ttccgaatgc ccctaaagcc tggctttgcg tatcaagaca gcgaaaagag ccggcacagc
     1801 cgtcgggcag gtaagggaat gttcaattaa tgtttaatgc ttaatgttta atgagtaatg
     1861 tattaatgcg taatgggtta attagcaatg gggtaattag tgatgactaa cgactaatga
     1921 ctcatagcca ataaacctac aacttaataa tcttaccggt aactgatttt tctttcgtgg
     1981 ctatcctgta aacatataat ccttttgata atgcattcaa atcaagtgaa ataatgtttt
     2041 caccctccgt ggagtcataa atcaggcgat aaacctgttt tcccatcata tcaagaattt
     2101 gaatccctgt tttttcagga cggccgacat taaaacagat ttcgatcttt tcggggaaag
     2161 ggtttgggta gcagaaatgc tttattgatg tgatcctttc tctttcaggc aggtcgacaa
     2221 agccaccggt ggttgtatgg aaaacggcat cagcccatgc gccccaaccg ttcagctcat
     2281 ccagaaaata gaaagaccag agatccgacg gaccttcaag gcatgtgtaa gatttccagg
     2341 tcaggccgcc atccagcgta tatcgcagcg gcgggccgat ccaacccgta ttcggatcga
     2401 taaacatgac ctcgtagtat tggtatacgc ttaacggaag ttgcacctcc caggttagtc
     2461 caccgttgcc ggtatgcaac actttatttc ctgcggccca gccattaagg ctatctgtaa
     2521 agaagatgga attgaaacgg gcagaattat ccgtatactg agaaatccag gtctgtccgc
     2581 catcgtttgt atgcaataat tcacacggaa tactgtacaa cgctatccat ccattgtttt
     2641 catcggtaaa gcataccgat ttaatgtcac tgtaatcttc aatgccacaa tcctgctcag
     2701 tccatgtcag ccctccgtca aaggtatgca ggagaatccc attgtcgcac ccgatccatc
     2761 cccggaggtt attcacaaag cacacatcat aaagagttcc tgttgtcggg ctggcctggt
     2821 aatcccagtc ggaaccaccg ttaacggtat gcaggatcgt tccggcagat ccgacgatcc
     2881 agccatgatc agcatcggta aatgttacgt cgaataaagt tccatcccca aagccaagag
     2941 tctgccaggt agctccctca tccgcagtac ccacggcagt gccgttgttt ccgaccatcc
     3001 agccgttctc cacatccgta aaata
//
LOCUS       DAOQXS010000385         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34773_length_3020_cov_3.496796, whole genome shotgun sequence.
ACCESSION   DAOQXS010000385 DAOQXS010000000
VERSION     DAOQXS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34773_length_3020_cov_3.496796"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKM34_10085"
     CDS             <1..946
                     /locus_tag="PKM34_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83981.1"
                     /translation="TGGWKREAFAVSAGQKTFKWAYEKNNMGSGGSDCAWLDYIILPS
                     PFALTIWAGSDNSVCNGNSYEINDAYGTDYELIEWISSGSGTFDDNTSMHPLYTPSGQ
                     DLSTGQVALTLNLWDDEGSMVTDEMQLGFRDVPSAPEQPDGLSYVDVATVETSDYTTD
                     IMEEADDYNWTIDPPVAGVIIGNENTATVSWNAEFTGVAYISVTGGNACGNGEVSAAL
                     EVQVVNSIVGLTEPDDPAVSIFPNPASGIIHVNLYSIGSYNPVVRLVNMIGIELFNSG
                     VVNNVSDIVIPVSGYAPGVYLLSVNTGDRQLTRKIIIK"
     gene            946..2727
                     /locus_tag="PKM34_10090"
     CDS             946..2727
                     /locus_tag="PKM34_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83982.1"
                     /translation="MIYRICAFSVILILNLSAGGQNRVTGISLLNYTGEIRYVPDVMG
                     FVVVADNLNLSVDQKQIQRDEIILFPGWPVSIGGSNERGGVYANLDGDEDFELIYPAG
                     TMLYALNIDGTAVEGWPRQLDHPTDGSAAFGDIDGDGAGEIVVTTHENATFAIGSVYA
                     FETDGTMVTGFPVATEGGGIRTPALSDLDLNGALEIVMTVRKWPDGFVYVFKGDGTMF
                     PGWPQRMDYVPGSAAAAGDIDGDDYPEIIAESYYRLHAYNRFGDLLPGFPYLPGENRV
                     FSYSTPVLADLDEDGNREIICGDHSIGDGTGAVHIVRHNGLSLPDWPRFTVSWVYSPP
                     SVADIDGNGLLDVAVGDQTLSETPVNRIYAWTAMTGEALPGFPVENVHGINSQILLAD
                     VDGDGMVELITDDNTSNGKYAGFNHDGTVAEGWPLSLDGTTFFINPFVTDINFDGTLD
                     ISGAGYIVGAGLTNVYLWNTGKDYVEENNPLPILQYNTRRNGVFGDTLMVGIRDHKEK
                     MTGKWSLSPNPARNIVSVNPPTGHSGNPVNITLFNSSGNKYFTQDYKENPPYLINLTG
                     FPPGIYYLKIEDEPTRRKILKLIILPK"
     gene            complement(2779..>3020)
                     /locus_tag="PKM34_10095"
     CDS             complement(2779..>3020)
                     /locus_tag="PKM34_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83983.1"
                     /translation="RDSYEYGDGDYNYDYYGYYNWDSFFVVEPGVEIELNVIKFFRIG
                     VGASYRITTNLSMDGLPKDMLNGFNAAVTFKFGKF"
BASE COUNT          750 a          731 c          782 g          757 t
ORIGIN      
        1 gacaggggga tggaagcggg aagcgtttgc tgtcagcgcc ggccagaaaa ctttcaagtg
       61 ggcttatgaa aagaacaaca tgggcagcgg cggttccgac tgcgcctggc tcgattatat
      121 catcctccca tcgccctttg ccctgacgat ctgggccgga agcgataact cagtatgcaa
      181 tggcaacagt tatgaaatta acgatgccta tgggaccgat tatgagctga ttgaatggat
      241 ctcctcggga agtggaacct tcgacgacaa taccagcatg catccgttgt atactccaag
      301 cggccaggat cttagcaccg gccaggttgc gctgacactg aacctgtggg acgatgaagg
      361 cagcatggtg accgatgaaa tgcagctcgg attccgtgat gtcccatcag ctcctgaaca
      421 acctgatgga ttgtcttatg ttgatgttgc cacagtggag acttctgact atacgacaga
      481 cataatggaa gaggctgatg attacaattg gactattgat cctccggtgg ccggcgtgat
      541 catcgggaac gaaaatacgg cgaccgtgag ttggaatgcc gaatttaccg gtgttgcata
      601 tatttcggtg accggcggca atgcatgcgg caacggcgaa gtttcggctg ccctggaggt
      661 gcaggtggtt aattccatcg ttggtttaac ggaaccggat gatccggctg tttccatctt
      721 ccccaatccg gcatccggaa tcattcatgt gaacctttat tctattggaa gttacaatcc
      781 ggttgttcgg ctggtgaaca tgatagggat tgagttgttt aattcgggag tggtgaataa
      841 tgttagcgac attgttattc ccgtttccgg atatgctccg ggagtttatc tcctctcggt
      901 caataccggt gaccggcaat tgacccgaaa aattatcata aaataatgat ctacaggata
      961 tgcgcttttt cggtcatctt gattctgaat ctctccgctg gcggacagaa tagagtgacc
     1021 ggcatatcct tgctgaacta taccggtgaa atccgctacg ttcccgatgt catgggattt
     1081 gtggtggtgg cggataatct caatctttct gttgatcaga agcaaattca gcgtgacgaa
     1141 atcatcctct ttcccggctg gcctgtgtcg attggtggaa gcaatgaaag gggaggggtt
     1201 tatgccaacc tcgacggtga tgaggatttt gagttgattt accctgccgg aacaatgtta
     1261 tacgcattaa atatcgacgg aacggcagtt gaagggtggc cccggcaact ggatcaccct
     1321 accgacggat cagcggcttt cggggatatc gatggcgatg gagcgggcga gatcgtggtg
     1381 accacccatg aaaatgccac ctttgctatc ggatcggttt atgcgttcga gacagatgga
     1441 acaatggtaa ccggattccc agtggcgacc gagggcggcg ggatccgcac cccggccctg
     1501 tcggacctcg acctcaacgg agcgctggaa atcgtgatga cggtcagaaa gtggccggat
     1561 ggcttcgtct atgttttcaa aggagatgga acaatgttcc cgggctggcc gcaacggatg
     1621 gattatgtcc ccggttcggc ggctgcggca ggagatatcg acggggatga ttaccccgag
     1681 atcattgcag aatcgtatta ccggctgcat gcctacaacc gtttcggcga cttattgccc
     1741 ggattccctt atcttcccgg cgaaaaccgg gtattttcct attccacgcc agtattggcc
     1801 gatcttgacg aagacggtaa ccgtgagatc atctgtggcg atcatagtat cggggatgga
     1861 acgggtgccg tgcatattgt acggcacaac ggcctttcat tacccgactg gccaaggttt
     1921 accgtaagct gggtctacag cccgccgtcg gttgccgata tcgacggcaa cggcctgctt
     1981 gatgtggctg tcggcgatca gacactaagc gaaacccctg tgaaccggat atacgcctgg
     2041 actgccatga ccggcgaagc gctgcctgga tttcccgttg aaaatgtcca cggtatcaac
     2101 agccagatcc tcctggccga tgtcgatggc gatgggatgg tcgaactcat taccgacgat
     2161 aacacctcga atggaaaata tgccggtttc aaccacgacg gaaccgttgc cgagggctgg
     2221 cccctttcac tggatggcac tacctttttt ataaatccct tcgtaaccga tatcaatttt
     2281 gacggaactc tcgacatttc cggtgcagga tatattgtgg gcgccggtct tactaacgtt
     2341 tacctctgga ataccggcaa ggattatgtg gaagagaaca atccccttcc catattgcaa
     2401 tacaataccc ggcgcaacgg tgtttttggc gatacgctca tggtcgggat ccgggatcat
     2461 aaagaaaaaa tgaccgggaa atggagctta agtccaaacc cggccaggaa tattgtttca
     2521 gtaaacccac cgacaggtca ttccggaaat cctgtaaaca tcactctatt caattcttca
     2581 ggaaataagt attttacaca agattataag gaaaatccac cctaccttat taatctgacc
     2641 ggtttcccgc caggtattta ttatttaaag atcgaagatg aacccacccg ccgcaagatc
     2701 ctaaaactga ttatacttcc aaagtaagtc gaatgaaccg acggctttag tcgtcggttc
     2761 atattgatta taaattcatc agaactttcc gaacttgaac gtcacggctg cgttgaaccc
     2821 gtttagcatg tctttcggca gcccgtccat actcagattg gtggtaattc tgtatgatgc
     2881 gcccacaccg atgcggaaga acttgatcac attgagttcg atttcaactc ccggttcgac
     2941 cacaaagaat gaatcccagt tataataacc ataataatca taattgtaat caccatcgcc
     3001 gtattcatat gagtccctgt
//
LOCUS       DAOQXS010000386         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_34877_length_3013_cov_3.705882, whole genome shotgun sequence.
ACCESSION   DAOQXS010000386 DAOQXS010000000
VERSION     DAOQXS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34877_length_3013_cov_3.705882"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKM34_10100"
     CDS             complement(<1..561)
                     /locus_tag="PKM34_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83984.1"
                     /translation="MKKQFLSSFFKLTLLVLMGTLIFVGCKDDDDDDPPIVVLDGFYI
                     KGAATALPDLADNGRMSVARNEVNQTDRADLFEMYIPIKAGTDGFNIIMVKGDTLKTY
                     GPGADFALVPEAELDVEEPKEGLWKGSLVETETKFTVPEDALYHVMFDRELMKIAIAK
                     VVWGVIGGATPGGWSESTPMTAGTFGT"
     gene            complement(683..>1893)
                     /locus_tag="PKM34_10105"
     CDS             complement(683..>1893)
                     /locus_tag="PKM34_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNQ83985.1"
                     /translation="DEESVNRMWEKFWTGITTCNQVLDMMKELPQNDALVAKMREVEV
                     LRAFYYYLLIDNYGDAPYLSSAANAPEKPYKAKREDIFDSLVTTVKNSLPYLKAIDNK
                     YMANRYMAYALLTKLYLNAEVYTGTPMWEEALIYCDSILAGPYAMEETNFMAPFLTNN
                     EKNKEIIFSIPYDEDNFQGFRLHMRTLHYQHNLKYDMPVGPWNGFCVVPTFFDTYDDS
                     DLRKSKYHIYGMQYDTKGDAIIDGETGQPLDISPYVPALYMQAADYTPQQIRTTGARI
                     GKYEIKTGAKENLSNDFPLFRITDFNLIKAELEIRLGRSGDEWIMPVRERAGVAELPP
                     TSGWTLDQLLAERGRELYVEGHRRQDLIRYGKWEDAWWEKEAHGTDRRTFPIPKWATD
                     ANPNLLENPR"
     assembly_gap    1894..1903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1904..2193)
                     /locus_tag="PKM34_10110"
     CDS             complement(<1904..2193)
                     /locus_tag="PKM34_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83986.1"
                     /translation="MKKISLLYLALFITLFSCTKLDEEVYDKIPGDLYPENENQIANL
                     SVEAYAKLKPLCDDEGWWFLAQEISSDELCGPTRGSDWFDGGKWVNVHRHTWS"
     gene            complement(2207..>3013)
                     /locus_tag="PKM34_10115"
     CDS             complement(2207..>3013)
                     /locus_tag="PKM34_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83987.1"
                     /translation="MSNRGIELYLQAFIISNTNFSYKSSLTASHNKTEILDLGDYFNE
                     EDGVRKEGYISGRGMVGEEYYVTGIIVGEETGSFYVPTFITLKDGKFIYESKTGGFTD
                     VLSDAKRTVVATAAPKVELGWSNTLTFRKRWNLDFALRSWIGNHVYNATEMFFDNPGN
                     LPSLNALPSAIEWYDQDRVTGSTIADIYVENASFLKLDYISLSYEFDVSKVNWLQGLS
                     LYVSANNVLTITGYSGVDPETTVNGLSFGIDQYNVYPKTRAYTAGLKVNF"
BASE COUNT          754 a          736 c          658 g          855 t
ORIGIN      
        1 tgtgccgaaa gttccggcgg tcattggagt gctttcgctc cagccacccg gggtagctcc
       61 accgatcacg ccccaaacca cctttgcaat agcaatcttc atgagttccc tgtcgaacat
      121 gacatgatac agagcatcct caggaacggt gaatttggtt tcagtttcaa cgagcgaacc
      181 tttccataag ccttctttcg gctcttccac gtcgagttcg gcttccggca ccagggcgaa
      241 gtcggctccg ggtccgtagg ttttcagggt gtcgcccttc accatgatga tgttgaatcc
      301 gtcggtaccg gctttgatcg gaatatacat ttcgaaaagg tcggcgcggt cggtctggtt
      361 aacttcattg cgggcaacgg acatacggcc gttgtcagcc aggtcgggaa gcgcggttgc
      421 ggctccctta atgtaaaaac cgtcaaggac tacgattggc ggatcatcgt catcatcatc
      481 cttacatcca acgaaaatca atgttcccat cagaacaagc agggtcagtt taaagaagct
      541 gcttaaaaat tgttttttca tacttaattt agttagatta gttaaaaaat tgaattgcaa
      601 taacattaat ttcaaactct ttggctttca aatgcgaaat tacaaataaa ttgattttat
      661 ttcaatattt aattcattca atttaccggg gattttccag taaattcgga ttggcgtccg
      721 ttgcccattt cgggatcggg aatgtcctcc ggtcggtccc gtgagcttct ttttcccacc
      781 aggcatcctc ccattttccg taacggatga gatcctggcg cctgtgccct tccacataaa
      841 gctctcttcc tctctcagcc agcagttgat ccagtgtcca accgctggtt ggcggaagct
      901 cagcgacacc ggcacgctcc ctgaccggca tgatccattc atctccgctt ctaccgagcc
      961 tgatctccag ttcagcttta atcaggttaa aatcggtgat cctgaataac gggaaatcgt
     1021 tgcttaaatt ttccttggcc cctgttttta tctcgtattt tccgatcctg gcgcctgtcg
     1081 tcctgatctg ctgcggagta taatcggctg cctgcatata aagcgccgga acataaggac
     1141 tgatatccag gggctgtccc gtttctccgt cgataatcgc atcacccttg gtatcatatt
     1201 gcattccgta aatatggtat ttggatttcc gaaggtccga atcatcatat gtgtcaaaga
     1261 acgtcggaac gacgcaaaaa ccgttccatg ggccgactgg catatcatat ttcaggttgt
     1321 gctgataatg gagcgtacgc atatgcagcc ggaaaccctg gaaattatct tcatcgtaag
     1381 gaattgagaa aatgatctcc ttgttctttt cgttatttgt caggaacggt gccataaaat
     1441 tggtctcttc catggcataa ggacccgcca ggatgctgtc gcaataaatc aaggcttctt
     1501 cccacatagg cgtacccgta taaacttcag cattcaggta aagctttgtg agcagtgcat
     1561 acgccatata gcggttggcc atgtatttgt tatcgatggc tttcaaatag ggtaatgagt
     1621 tttttaccgt agtaacaaga ctgtcaaaga tatcttcccg ttttgccttg taaggttttt
     1681 ccggtgcatt ggccgcgctc gacaggtacg gggcatctcc ataattatca atcagcagat
     1741 aataatagaa cgctctcaac acctccactt cgcgcatttt ggcgacgagg gcatcattct
     1801 ggggaagctc tttcatcata tccaggacct ggttgcaggt tgtaatgcct gtccagaatt
     1861 tttcccacat tcggttcacg ctctcctcat cgtnnnnnnn nnngaccatg tatgccggtg
     1921 aacattcacc catttacctc cgtcaaacca atcgcttccc cgtgtcgggc cacaaagttc
     1981 gtcggatgaa atttcctggg ccaaaaacca ccatccttca tcatcgcaca acggtttaag
     2041 tttggcatac gcctcaacgc tcaggttggc aatctggttc tcattttccg gatatagatc
     2101 gccagggatt ttatcataca cctcttcatc cagtttggtg caggagaaaa gggtaatgaa
     2161 caatgctaaa tataataaac tgatcttttt catagctttt ctgggattag aagttaacct
     2221 tcaggccggc cgtataagcg cgggttttgg gataaacatt gtattgatcg atgccgaatg
     2281 acaggccgtt gacagtggtt tcaggatcca caccggaata gccggtgata gtaagtacgt
     2341 tgttggcaga cacatagagt gaaagtccct gcagccagtt caccttcgac acatcaaact
     2401 cgtacgacag tgaaatatag tccagcttaa ggaatgaagc gttctcaacg tagatgtctg
     2461 cgatcgtcga tccggtaacc cgatcctggt cataccattc aattgccgag ggcagtgcat
     2521 tgagtgaagg taaattgccc gggttgtcga aaaacatttc cgttgcattg taaacatggt
     2581 ttccgatcca ggaacgtaag gcaaaatcga gattccagcg tttgcggaag gtgagggtat
     2641 tcgaccatcc aagttcaact tttggcgctg cagtggctac tacggtccgt ttggcatcgc
     2701 tcagcacgtc agtaaatcct cctgttttgg attcatagat aaatttccca tccttgagtg
     2761 ttataaaagt cggaacatag aaagaaccgg tctcttctcc aacaataatc ccggtaacat
     2821 aatattcttc accgaccatc cctcttcctg aaatatatcc ctcttttctg acgccgtctt
     2881 cttcattaaa ataatcccca agatcaagaa tttcggtttt attatgagaa gccgtcagac
     2941 tcgatttata gctaaaattg gtatttgaaa tgataaatgc ctgcaggtaa agctctatcc
     3001 cccggttaga cat
//
LOCUS       DAOQXS010000387         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_35244_length_2990_cov_2.886882, whole genome shotgun sequence.
ACCESSION   DAOQXS010000387 DAOQXS010000000
VERSION     DAOQXS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35244_length_2990_cov_2.886882"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKM34_10120"
     CDS             complement(<1..872)
                     /locus_tag="PKM34_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HNQ83988.1"
                     /translation="MKNIISVLLFLVFLAGTHAQSQPDSVVQKIIEIGKADNRTMEHL
                     DVLCNRIGGRPIGSSAYESATIWAATKFDEWGMEVILDKVGELPVGFNRGPWSGRMLS
                     DEGMILHFATPSYTAGTKGVQRGHVLIEPRTQAEFDRMKGQLEGAWVLILGENDGWPI
                     DISAHADSLRDSIKAANAAIEEENNKIRRENRDNKEKGLPEKELKELNEEPALFYREM
                     REAGILGIIQSSKTPIRVMYDRKNIMDMTFDNLPAVPDIKLDEHQYAVIKQMAEERRY
                     FQLEFDIRNHFRPGP"
     gene            1077..>1309
                     /locus_tag="PKM34_10125"
     CDS             1077..>1309
                     /locus_tag="PKM34_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ83989.1"
                     /translation="MKSLIVALLLVISAGLTTAQESKNIVLNPPGADRGLSIMKSLSL
                     RASVNEYKPDDLALQDLSDLVWAANGINRPENG"
     assembly_gap    1310..1409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1410..1583
                     /locus_tag="PKM34_10130"
     CDS             <1410..1583
                     /locus_tag="PKM34_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ83990.1"
                     /translation="AAMDAGIVSQNIALFCAGTGLGTRPRAWMDKDLLRDALHLKDSQ
                     HLMLNHPVGYLKD"
     gene            complement(1803..>2990)
                     /locus_tag="PKM34_10135"
     CDS             complement(1803..>2990)
                     /locus_tag="PKM34_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNQ83991.1"
                     /translation="KLALSPDEKQLAICGNSNSIMVYDFPSAFLKGELTDEHKKDPIF
                     ITFNKYGDQLVSLGLDKKIVYWDLNRFKSVTSFEISPNTISGSGFDVLSREASHDKQI
                     LVTGIEEIKLAKGGNSMVFKYFYSFYNLEDHSEIKLLEGNTKKLEIICMTPDKKYMIS
                     DNSSLQTAKISFWNIQTGVIDYDYSFKVNAEVTSIAVSRSGKWLAVSYTRDNALSASE
                     INLFEMTGIGAYDNQSFTDNVMESSGSGFGSSIRYTTPKTPLIEIGQKRVIAVINLDN
                     IGLTDDVAKTATYLLESKLGNNDKVVLTERNQVEKLVAEMQYQQSGMTTSDVVSLGEQ
                     LNANYILTGSLNKLGNTIILSIKLINIETAQIIGTREVQCDNATVEDIPDMIMLIAPS
                     IVK"
BASE COUNT          682 a          702 c          667 g          839 t
ORIGIN      
        1 ggcccggggc ggaaatggtt gcggatgtcg aattcgagct ggaaatagcg gcgctcttcg
       61 gccatctgct ttatgaccgc atactggtgc tcatccagct ttatatcggg aaccgccggc
      121 aggttatcaa aggtcatgtc catgatgttc ttccggtcgt acattacgcg gatcggcgtc
      181 ttcgacgact ggatgatgcc caggatgccg gcttcgcgca tttcgcggta aaatagcgcc
      241 ggttcctcgt taagttcttt cagctctttt tcgggcaacc ctttctcttt gttatcccgg
      301 ttctcccgcc tgatcttgtt gttctcctct tcgatcgcgg cattggctgc tttgatcgaa
      361 tcgcgcaggc tgtcggcatg agccgagata tcgatgggcc agccgtcgtt ctctcccagg
      421 atcagcaccc atgcgccttc cagttgtcct ttcatccggt cgaactcggc ctgggtgcgc
      481 ggttcgatca gcacatggcc gcgctgcacg cctttggtcc ctgcggtgta agagggggtg
      541 gcgaagtgga ggatcatccc ttcatccgac agcatgcgcc ctgaccacgg gccgcggttg
      601 aagccgacgg ggagttcgcc cactttatcc aggatcacct ccattcccca ttcgtcgaac
      661 ttcgtggcag cccagatggt tgcgctttcg taggcgctgc tgccgatcgg ccgcccgccg
      721 atccggttgc aaagaacgtc gagatgctcc atcgtacggt tgtcggcctt tccgatttcg
      781 atgatctttt gaacgacgga atcgggttgg gactgggcat gagtgccggc aaggaagact
      841 agaaagagca ggacgctgat gatgtttttc ataatatcgg gttatattca ctgtgtatga
      901 caaaattacc aagtaaaacc ggatgaaaat taaaataatt cgcgtaaatt cgttgcattc
      961 gcgttcgatt atagaacacg aattacgcgg atttagacga attttcacga ataatattag
     1021 ttccagaaca agatgaaatc acgtttctca ctcaacagtt tcaaaataca attctcatga
     1081 aaagtctgat agttgcgtta ttactggtca taagtgcggg tttgaccacc gcccaggaat
     1141 ccaaaaacat cgtgttgaat cctcccggcg ccgaccgggg cctcagcatc atgaaatcct
     1201 tatcgctgag ggcatcggtg aatgaataca agcccgacga cctggccctg caggatcttt
     1261 ccgacctggt gtgggcggcc aacggcatca accgcccgga gaacgggaan nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggcaatgga cgctggcatt gtttcgcaga
     1441 atattgcgtt gttctgcgcc ggaacaggac tgggaacgcg cccccgcgcc tggatggata
     1501 aagaccttct ccgcgatgcg ctgcacctga aggattcgca gcacctgatg ctgaaccacc
     1561 cggtagggta tttgaaggac tgaccggcag ggattgaata atttatttgc ttttttaccg
     1621 gttcttgggt atttttccgg tactaaaacc gtggttgcat tttaatcagc caatataaac
     1681 ttcttgccat aaacgcctct gacagaaaaa tgattttgcc atcgataaat tggctgggtt
     1741 ttccgaaacg gaaggaatag attcagaatc agggaactaa atagaaatga cagctattaa
     1801 tttcatttca caatcgacgg ggcgatcagc atgatcatat ccggtatatc ctctaccgtt
     1861 gcattatcgc actggacttc ccttgtgccg atgatctgcg ctgtttcgat attaatcagt
     1921 tttatgctta gaatgattgt atttccaagt ttattgaggc ttcctgtcag gatatagttt
     1981 gcattcaatt gttcaccaag gcttacgaca tccgatgtgg tcatacccga ctgctggtac
     2041 tgcatttcag caaccagctt ttcgacctgg tttctttctg taagaacaac tttatcatta
     2101 ttacccagtt ttgactccag caggtatgtg gccgttttcg ctacatcatc cgtcaggcct
     2161 atattatcga gattgatcac tgctatgact ctcttctgtc ctatttcgat caaaggtgtt
     2221 tttggtgtgg tataccttat ggaactcccg aaaccggaac cgctgctttc catgacatta
     2281 tccgtaaacg actggttgtc atatgccccg atgccggtca tttcaaacag gtttatttca
     2341 gaagcagata aagcattatc ccttgtatag cttacggcaa gccatttgcc gctccgtgat
     2401 accgctatgc tggttacttc agcgttaact ttaaatgagt agtcgtagtc gatcacaccg
     2461 gtttgaatat tccagaacga tattttagca gtctgcaaag aagagttgtc agaaatcata
     2521 tatttcttat caggtgtcat acatatgatc tccagtttct ttgtatttcc ttccaggagt
     2581 tttatttcac tgtgatcttc caggttataa aatgaataga agtatttaaa gaccatgctg
     2641 ttgccgcctt ttgccaattt aatctcttca atcccggtaa ccagtatttg cttgtcatga
     2701 cttgcttccc tggataacac atcaaaccct gatccactaa tggtattcgg acttatttca
     2761 aatgaagtca ctgatttaaa gcggttcaaa tcccagtaga caatcttttt atccagccct
     2821 aacgatacca gctgatcacc atatttatta aatgttataa atattgggtc tttcttgtgt
     2881 tcatctgtca actccccttt gaggaatgct gaagggaaat cgtaaaccat tatactgttg
     2941 ctgttcccgc aaattgccag ttgcttttca tcgggactaa gcgccagttt
//
LOCUS       DAOQXS010000388         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_35518_length_2973_cov_3.620973, whole genome shotgun sequence.
ACCESSION   DAOQXS010000388 DAOQXS010000000
VERSION     DAOQXS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35518_length_2973_cov_3.620973"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKM34_10140"
     CDS             <1..1131
                     /locus_tag="PKM34_10140"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNQ83992.1"
                     /translation="IDGNDTITGFMVQDTLNGFNNVFDFSFSSSLSTKVYGLVQFGRK
                     FPVRAIRHVLTPSVSFSYRPDFSTDFWGYFDSYIDKDGKEKVYSKYEGAIFGAPPMGE
                     SGAIGFSFANNLEMKVRSRKDTITGTKKVSLIDNFTIGFSYDFARDSLNLSTILMQGR
                     TRLFKNLDIGYRADLDPYVLDSAGKNLNRFEWDVNRRLLRLKQTNWSVSLNWRLNSKE
                     LGKNKEKPGGTPVTTGAPQVPTGDESEMTEIADNPDQYIDWSIPWDLTLSYSFNYTVV
                     RRYPEYILYKDKKIVQTLGVSGNISVTPKWKIGFMTGWDFEDKDLSYTSISIYRDLHC
                     WEMRFNWIPTGYQKSWNFQINAKASLLQDLKLNKKKDFRDNF"
     gene            1183..1563
                     /locus_tag="PKM34_10145"
     CDS             1183..1563
                     /locus_tag="PKM34_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNQ83993.1"
                     /translation="MKKIISTPDAPKAVGPYSQAVEFNGMLFISGQIPIDPAIGKIVD
                     GGIREQTEQVMKNIGVILKEAGYDYKDVVKSTCLLNDMDNFAAMNEVYARYYPENPPA
                     RAAYGVVRLPLGAMVEIETIAMKK"
     gene            1702..2973
                     /locus_tag="PKM34_10150"
                     /pseudo
     CDS             1702..2973
                     /locus_tag="PKM34_10150"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
BASE COUNT          819 a          685 c          795 g          674 t
ORIGIN      
        1 attgatggaa atgataccat cacggggttt atggttcagg ataccctcaa cgggttcaac
       61 aatgtgtttg atttcagttt ttcatcttct ctcagcacaa aggtttacgg actggtgcag
      121 tttgggcgga agttcccggt cagggcaata cggcatgtgc ttaccccctc ggtctctttt
      181 tcttaccggc ccgatttcag taccgatttc tggggttatt ttgacagtta tattgataaa
      241 gacggaaagg agaaagttta ttccaaatat gaaggggcga tattcggggc tccgccgatg
      301 ggtgaatcgg gcgcgatcgg gttcagtttt gccaataacc tggagatgaa ggtgagatcc
      361 aggaaagata ctatcaccgg tacaaaaaag gtcagcctga tcgataattt caccatcgga
      421 ttttcgtacg attttgccag ggattcactc aacctgtcga ctatactgat gcagggaagg
      481 acccggcttt tcaagaatct tgacatcggc taccgtgccg atcttgaccc ctatgttctc
      541 gattcggcgg ggaagaacct aaaccggttt gaatgggacg tcaaccggcg actgctcagg
      601 ctgaagcaga ccaattggtc ggtcagtctc aactggcggc tgaattcgaa agaactgggc
      661 aagaacaagg aaaaacctgg tggaacaccg gttacaacag gagccccgca ggttccgacc
      721 ggggatgaga gtgaaatgac ggagatcgcc gataatccag accagtacat cgattggtcg
      781 atcccgtggg acctgaccct gagctattct ttcaattata cggtggtgcg gagatatccc
      841 gaatacatcc tgtacaagga taaaaagatc gtccagacac tgggagtatc aggcaatatc
      901 agcgttacac cgaagtggaa gatcgggttt atgaccggtt gggattttga agataaagac
      961 ctgtcgtata cttcaatcag catctaccgc gaccttcatt gctgggagat gcgtttcaac
     1021 tggatcccca caggttacca gaagagctgg aacttccaga tcaacgccaa agcctctttg
     1081 ctgcaagacc ttaaactcaa caagaaaaag gatttcaggg ataatttcta attctgaatg
     1141 aacctgaata tttataaaat aaaacattaa aaatactaca ttatgaaaaa aatcatcagt
     1201 actcccgacg ctccgaaagc cgtaggaccg tatagccagg ctgtagaatt caacggaatg
     1261 ttatttattt ccggccagat cccgatcgat ccggccatcg ggaagatcgt cgacggaggg
     1321 atcagggaac aaaccgaaca ggtaatgaaa aatattggcg tgatcctgaa agaagccggt
     1381 tacgattaca aagatgtcgt caaatcaacc tgcctgctga acgatatgga caatttcgct
     1441 gccatgaatg aagtgtatgc ccggtattat cccgaaaacc cgccggcccg ggcagcgtat
     1501 ggcgtggtcc gcctgcctct cggagccatg gtggagatcg agacgattgc aatgaaaaaa
     1561 taaacattaa gaattaaaaa ttaggaatta atgaaaaagt gtatcataac tcttctgtta
     1621 ggtttcttgc tctctaacct gtcaattgcc gatgaaggaa tgtggattcc cagcctgatc
     1681 gggcagcaga agctggacga tatgaagaaa cggggcctga agctttccgc cgaggatatt
     1741 tacagcatca accagtccag tttgaaagat gccgtggtgc tcttcggtcg cggttgcacc
     1801 ggggcgatcg tatccgctga aggcctgctg ctcaccaacc accattgcgg tttcggcagc
     1861 ctccagcgcc acagcgcagt ggagcacgac tatttgcgcg acggattctg ggcgatgagc
     1921 cgcgaagaag agctgcccaa tgcaggactc tccgtaactc tgctggtcag gatggaggac
     1981 gtaacggatc gtgtcctggc atcggttaaa gaggggatga gcgaggccga ccggcagaaa
     2041 gccatcgatg atgcagccaa ggcgattgtg gaggaagcgg tcaaagaaac acactaccag
     2101 gccgaggtcg aaccgctgtt caacggaaat cagtattttc tctatgtttc ggaggttttc
     2161 aaggatgtcc gcctggtagg ggcacccccg tcggcagtgg gcaattttgg cggcgatacc
     2221 gataactgga tgtggccgag gcatacgggc gacttcagcg tcttccggat tatgccgaca
     2281 gcaacaatat acccgcggag tattcgaaga ataacaagcc ttatcagcca aaaaagtatt
     2341 ttgaagtttc actggatggc gtcgataagg gtgattttac gatggtctac ggatatcccg
     2401 cccggacaca gcaatatctg ccatcttaca tggttgaact gatagccgaa caatcgaatc
     2461 cggagaagat agccatacgt cgtgaaaagc tcgacatcat gggagctgca atgaactccg
     2521 accagaagat caggattcag tatgccagca aatatgccgg ggtggcgaat gcctggaaga
     2581 agtggatggg tgaaagcaaa gggttgcaac gtttcgatgc cgtagagaaa aaacaggcat
     2641 ttgagaagga tttttcggcc tgggccagta tgaacgcacc gcagtacagc gggatccttc
     2701 ctgcttacca ggcgctgacg gatgaactga caccctgcca gctctgggtc gaccgttttt
     2761 cagaagccgt atggagcctc gatatcatcc gcctggcagc cggctacagg aacctggccg
     2821 gaatggacaa aacagccggc gaagacctgc ggaaagaagc ggaccggctt aaaggcaatc
     2881 tgaacggctt ctttaaggat tatgatcagg caacggataa gaagctcttt gcctccatgc
     2941 ttgggttttt ccggaataat tcgcaacaga agg
//
LOCUS       DAOQXS010000389         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_35898_length_2950_cov_3.936097, whole genome shotgun sequence.
ACCESSION   DAOQXS010000389 DAOQXS010000000
VERSION     DAOQXS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35898_length_2950_cov_3.936097"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKM34_10155"
     CDS             complement(<1..464)
                     /locus_tag="PKM34_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ83994.1"
                     /translation="METNESKGKCPVTGATSRHSVGSGGTKNRDWWPNQLKLNILRQN
                     SSKSDPMGEDFNYADEFKKLDYEGLKNDLRKLMTDSQEWWPADFGHYGPLFIRMAWHS
                     AGTYRTGDGRGGAGSGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGRKISWA"
     gene            complement(618..1037)
                     /locus_tag="PKM34_10160"
     CDS             complement(618..1037)
                     /locus_tag="PKM34_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ83995.1"
                     /translation="MKSTEYRNKLVECGLKVTPQRMAILQAIIKLDNHPTADQIIELI
                     RKNNPNISTATVYKVLDTFAACGLIKRVTTATGIMRYDAHLDSHHHLYYTDSDKIEDY
                     RDEELSRLLETYFKQKNIPGFEIEDLKLQITGKHTDN"
     gene            complement(1147..2379)
                     /locus_tag="PKM34_10165"
     CDS             complement(1147..2379)
                     /locus_tag="PKM34_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ83996.1"
                     /translation="MVKYYLLSIFVFLSALSTAQWGPVNNGISNLSFGVKLLGSSDAY
                     IFSGTLADARMYRSGDNGNNWDEIDPPVTGNVPECGFFFEDKYFAGLNSSQECIFYST
                     DDGSSWNSVSGGPQTTWVRGFFSLSGDIFAFTSSAGIYKSTDGGSNWEPANSGLDNYN
                     VIRMESINSKLLAATIGGGVFASTDNGETWFPSNNGIAGSDLNATLVWRMGAGLFYME
                     QGGAAYTSNDEGANWSAWIKPAVMGLAVNEIYRTGNHLYMESRHFAGGLKDSVYYSIN
                     EGFSWDNITDNLSASDLNASGITEFEGYAFIAYNIISPGLGIYRRGISTGTVEMNNPV
                     SIEIYPNPFREKISFASFSRNNIKQINLFDNLGRLVLSENGSETVNTSGLPGGTYIVA
                     VVLADNTTIRRKLIKENR"
     gene            complement(2596..>2950)
                     /locus_tag="PKM34_10170"
     CDS             complement(2596..>2950)
                     /locus_tag="PKM34_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNQ83997.1"
                     /translation="SASYISGCDFTGSDMTGIVVKSGGFEKNILTDAVLDRSSFIDTQ
                     LIDVIFEGTLKDCYFENCSFNRVKFQDARIINTFFKNKSLKRIRFINCQVDRMTYAFL
                     KNGKADLTGIMLLEE"
BASE COUNT          759 a          676 c          658 g          857 t
ORIGIN      
        1 gcccaggaga tcttccggcc atatttctgc ttgatcggcc agagcagccg gcgggctttg
       61 tcgaggttca cgttgtcggg ccagctgtta aggggtgcaa aacgctgctg tccgctcccg
      121 gcgccgccgc ggccgtcgcc ggtgcggtat gtgccggcgc tgtgccaggc catccggatg
      181 aacaacgggc cgtaatggcc gaaatcggcc ggccaccact cctgcgagtc ggtcatcagc
      241 ttccggaggt cgtttttcaa tccttcataa tcgagctttt taaactcatc ggcataatta
      301 aaatcttcac ccatcgggtc ggatttcgat gaattctgcc ttaaaatatt cagctttaac
      361 tggtttggcc accagtcacg gttctttgtc cctcccgaac ccacactgtg cctggaggta
      421 gccccggtta cagggcattt tcctttgctc tcatttgttt ccattattta taagtttaat
      481 ttaagttatt tgtaaatagt tcattattca tttttatgcc accaaaacac taaagatatt
      541 tgattgatgt aaagcatttt gtgagctttc ctgttttaat gtttttgtgg cttataattc
      601 ttcttgtttt ttaaaaatca attatctgta tgttttccgg tgatctgaag cttcagatct
      661 tcaatttcaa aacccgggat gtttttctgc ttaaaataag tttcgagcaa ccggctgagt
      721 tcctcatctc tgtaatcctc gatcttgtcg gaatcggtat agtagaggtg gtgatggctg
      781 tcgagatggg catcatatct cataatgccg gttgccgtgg tgaccctttt gatgaggccg
      841 catgcggcga aggtatccag cactttgtag accgtggcgg tagagatatt cggattgttt
      901 ttacggatca gttcgatgat ctgatcggcg gtaggatggt tatccagttt gatgatcgcc
      961 tggaggatgg ccatccgttg aggcgttact ttcaaaccgc attccaccag tttattcctg
     1021 tattccgttg acttcataat gaattattat catataagaa taattctctt ttaagaatta
     1081 acaaagataa taataaaaag ttcagaagtc aagtctttcg ggacgccatc ctttgggacg
     1141 ccatccctac ctgttttctt taatgagttt ccgccggatg gtcgtattat cggccagaac
     1201 cactgctacg atataggttc cgccaggtaa ccccgaggta ttcactgttt cgctgccatt
     1261 ttccgaaagc acgagcctcc ccaggttgtc gaacagattg atctgtttga tgttgttacg
     1321 ggagaaactg gcaaatgata tcttttcacg gaagggattg gggtagattt caatgctgac
     1381 cggattattc atttccactg taccggtaga gatacctctg cgatatatcc ccaggcccgg
     1441 tgaaatgata ttataggcga taaatgcata tccctcaaac tccgttatac cggaagcgtt
     1501 caggtctgaa gcggataaat tatccgttat attgtcccag gagaatcctt catttattga
     1561 ataatacaca ctgtctttca atcctccggc aaagtgacgc gactccatgt aaagatgatt
     1621 gcctgtgcga tatatttcat tgactgccag gcccataacc gcaggtttga tccaggccga
     1681 ccagttagct ccttcatcat tactggtata agcagcgccg ccttgctcca tatagaacaa
     1741 accggcaccc attctccaga ccagtgtggc attcaggtcg gaaccggcaa taccgttatt
     1801 tgagggaaac caggtttcgc cgttatctgt cgaagcaaaa acgcctccgc cgatggttgc
     1861 ggccaggagt tttgaattga tcgattccat ccggatcaca ttgtaattat ccaatccgga
     1921 attagcgggt tcccagtttg agccgccatc ggtcgattta taaattcccg cactggatgt
     1981 aaaagcaaag atatctcccg ataaactgaa aaaacctctt acccaggtgg tctgcggccc
     2041 tcccgaaacg ctgttccagg aggaaccgtc gtcggtggaa tagaaaatgc actcctggga
     2101 tgagtttaat cctgcaaagt atttgtcttc aaaaaagaag ccgcattccg gcacattgcc
     2161 cgttaccggt ggatcgattt catcccagtt attcccattg tcgcctgaac gatacattct
     2221 ggcgtcggcc agggttccgg agaaaatata ggcatcggat gaaccaagta attttactcc
     2281 gaaggacaaa tttgaaatgc cgttgttgac cggcccccac tgggcggtgc ttaaagcgct
     2341 gagaaagaca aagattgata agaggtagta ctttaccata ttcggctaaa tattaatgat
     2401 tagttgttta actggtcatg aaaacaactc atcagccgaa aatgttcatc cggatttctt
     2461 tttcagattt cgaaacatgc cgcttaaatt gtcgagaaaa gacaggaaaa aaaagagtta
     2521 atacggttta tttcttacag tatgtttaac aaacaggata ttgaagttgt tcagtaataa
     2581 aaaccggatt ccctttcatt cttccagcag catgataccg gtcaggtcgg ctttcccgtt
     2641 tttcaggaaa gcataagtca tccggtccac ctggcagttg ataaagcgga tccgcttcag
     2701 gctcttgttc ttaaagaaag tatttatgat ccttgcatcc tggaatttca ccctgttaaa
     2761 ggaacagttc tcaaaatagc aatctttcag ggtgccttcg aaaatgacat caatgagctg
     2821 agtatcaata aaggaggaac ggtctaacac agcgtcagtc aaaatatttt tttcgaaacc
     2881 acctgattta acaacaatcc cggtcatatc tgaaccggta aagtcgcaac cgctgatgta
     2941 gctggctgaa
//
LOCUS       DAOQXS010000390         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36247_length_2932_cov_4.397636, whole genome shotgun sequence.
ACCESSION   DAOQXS010000390 DAOQXS010000000
VERSION     DAOQXS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36247_length_2932_cov_4.397636"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..411)
                     /locus_tag="PKM34_10175"
     CDS             complement(94..411)
                     /locus_tag="PKM34_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ83998.1"
                     /translation="MKTSYVYIITNKNRTVFYTGLTANLTNRMIRHKAGKGSKFCAKY
                     NVNTLVWYETHIHIMNAIRREKQIKHWNRQWKIDMIRARHPEMKDLLAKQGGSGAGGS
                     SPG"
     gene            complement(737..1966)
                     /gene="hydF"
                     /locus_tag="PKM34_10180"
     CDS             complement(737..1966)
                     /gene="hydF"
                     /locus_tag="PKM34_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNQ83999.1"
                     /translation="MKADKGRELKPHIGIFGRRNNGKSSLINALAGQDVAIVSAQPGT
                     TTDPVRKSVEIFGIGPAILVDTAGIDDSGDLGELRIRKTLQVIKAIDLAIIVITDNSI
                     SQFENELIRQFGNYEVPYIFVHNKSDLVELSEETRKIVQKEFTTDITDFSTLNPANKE
                     QLIGMIIRHMPETAYTSQSLLGGLLNRGDIVMLITPIDNEAPEGRMILPQVQAIRDVL
                     DNDCINVVLKETEVEHFLKTTGIKPRLAVTDSQMFGRIDKIIPKDIPLTGFSVLLARH
                     KGPFGKYMEGTPKIDQLNDGDRVLILESCTHQVNCDDIGRYKIPTWLSEYTGKKLEFD
                     IVAGLDSFESDFRKYALIVQCGGCMITRKQVHSRLKEAIEAGIPITNYGMAIAWTKGI
                     YQRAIEPFAAVSPLARG"
     gene            complement(2090..>2932)
                     /locus_tag="PKM34_10185"
     CDS             complement(2090..>2932)
                     /locus_tag="PKM34_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ84000.1"
                     /translation="DDEVIDRALFALRRDFGSVVIQSGERTDRVFIDMLTRVIKAIRE
                     RSEGTLGITLSCGEQSLETYQQWFDAGAHRYLLRIETSSEELFRKIHPDNEFHRFEDR
                     MQALENLRKAGFQTGTGVMIGLPGQTPENLADDLLFFRSFDIDMIGMGPYIPHADTPM
                     AGWKTRLLKPADRFELSLRMIALARIMLKDVNIAASTALQTLDPDGRIKAILAGANVF
                     MPNMTPKIYAGDYNLYEGKPQVMDDPMIIVNELEKRLRGLGESIGYSKWGDSKHFFKR
                     VIKN"
BASE COUNT          586 a          796 c          759 g          791 t
ORIGIN      
        1 ggaattgcga attgctatgc cgtttgcagc cgcgccgccc tggttcctct ctccacgcgc
       61 caaccccgtc attcccaagc cagcgtgaat tccttagcca ggcgatgacc cgccagcccc
      121 ggagcctcct tgcttcgcga gcagatcttt catctccgga tgcctggccc ggatcatatc
      181 gatcttccac tgccggttcc agtgcttgat ctgcttttcg cgcctgatgg cattcatgat
      241 atggatatgg gtttcgtacc agaccagagt atttacgttg tattttgcgc aaaacttcga
      301 tccctttccg gccttgtggc ggatcattcg gttagtgaga tttgcggtca gcccggtata
      361 aaataccgtc cggtttttat tggtaataat gtatacgtat gatgttttca tacctattaa
      421 tccggaattg tgctaaaaaa tacctggtag ggttgaaata attttgtatg tagttggcat
      481 tgagtgtgat gcggaggctg gtagtcccct ctttcgaggg gatgacgggt tccctctttc
      541 gaggggatga cgggttccct ctttcgaggg gatgacgggt tccctgaatt taggtggttc
      601 cccgcttacg cggggatgac gttgtaccgc tggaaataga ggaaacagag cggcggcgat
      661 gcaaagttga ttgctattat aaaagtcatc agcatcgccg ccgctatttt tttctctttt
      721 atccggtgca gtagcgtcat ccccgcgcca gcggggacac ggcggcaaac ggctcaatcg
      781 ctcgctgata gatccctttc gtccacgcaa tcgccatccc gtagttcgtg atgggaattc
      841 cggcctcaat cgcttctttc agccggctgt ggacctgctt tcttgtgatc atgcacccgc
      901 cgcactggac gatcagggca tatttcctga aatcgctctc gaagctgtcg agcccggcga
      961 cgatgtcgaa ttcgagcttc tttccggtat attcgctcag ccaggtgggt attttgtacc
     1021 ggccgatgtc gtcgcaattc acctggtgcg tgcagctctc caggatcagc acccggtcgc
     1081 cgtcgttgag ctggtcgatc tttggcgtcc cttccatgta tttgccgaac ggacctttgt
     1141 gacgcgccag cagcacgctg aaaccggtca gcgggatatc tttcgggatg attttgtcaa
     1201 tcctcccgaa catctggctg tcggtcacgg ccagccgggg ctttatgccg gtggttttca
     1261 gaaaatgttc gacctcggtc tcttttaaaa caacgttaat gcaatcattg tcaagcacat
     1321 cacggatagc ctgcacctgc ggcaagatca tccttccctc gggcgcttcg ttgtcgatcg
     1381 gggtgatgag catcacaata tccccgcggt tcagtaatcc gcccagcaat gactggctcg
     1441 tgtacgccgt ttcgggcata tgccggatga tcatcccgat cagttgttct ttattcgccg
     1501 gatttaaggt ggagaaatct gtgatatccg tggtgaattc tttctgcacg atcttcctgg
     1561 tttcttccga taattcaacc agatcagatt tattatgaac gaaaatatag ggaacttcat
     1621 aatttccaaa ttgccggatt aactcatttt caaattgact gattgaatta tccgtaataa
     1681 cgatgatggc caggtcgatg gccttgatca cctggagggt tttgcggatg cgcagctcgc
     1741 ccaggtcgcc gctatcatct atcccagcag tatcgaccag gatagccggg ccaatgccga
     1801 agatctcgac cgatttgcgc accgggtcgg tggtggtgcc gggctgcgcg gagacgatgg
     1861 ctacgtcctg cccggccagg gcgttgatca gggagctttt cccgttgttg cgcctgccga
     1921 atatgccgat atgcggcttg agttcacgtc ctttgtctgc tttcattgtt ttgttttaac
     1981 ttacagtttc ctctccgcct tcggcggatc ggaatgacga tgcttgctat atagaaagaa
     2041 aagcggcgcg gcatccgtca tcccggcgta agccggaatc tctatatttt caattcttaa
     2101 taactctctt aaagaaatgc ttcgaatcgc cccacttgct gtagccgatc gattctccca
     2161 gccctctcag cctcttttcc agttcattca cgatgatcat cgggtcgtcc atcacctggg
     2221 gtttgccctc atagagattg taatcgccgg catagatctt cggcgtcatg ttcggcatga
     2281 acacattggc cccggccagg atcgccttga tccggccgtc ggggtccaat gtctgcaaag
     2341 cagttgatgc agcaatgtta acatctttca gcatgatccg tgccagggcg atcatgcgca
     2401 ggctcagctc gaagcggtcg gccggtttca atagcctggt tttccaccct gccatcggtg
     2461 tgtcggcatg cggaatatag ggccccatgc cgatcatatc gatatcgaag ctgcggaaaa
     2521 acagcagatc atcggcgagg ttttccggag tctggccggg caggccgatc atcaccccgg
     2581 tgccggtctg gaatccggcc ttgcgcaggt tctccagcgc ctgcatacga tcctcaaacc
     2641 ggtggaattc attgtcggga tgtattttcc ggaaaagttc ttcggatgag gtctcaattc
     2701 tcagcaaata gcggtgagcg cccgcatcga accattgctg gtaggtttcc aggctttgtt
     2761 caccgcaact cagcgttatc ccgagcgtgc cttccgatcg ttccctgatg gctttgatca
     2821 cccgggtcag catatcgatg aacacacggt ccgttctttc cccggattgg atgaccacgg
     2881 agccgaagtc cctgcgcaaa gcaaaaaggg ccctgtcgat aacctcatca tc
//
LOCUS       DAOQXS010000391         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36280_length_2931_cov_3.170723, whole genome shotgun sequence.
ACCESSION   DAOQXS010000391 DAOQXS010000000
VERSION     DAOQXS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36280_length_2931_cov_3.170723"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1073
                     /locus_tag="PKM34_10190"
     CDS             84..1073
                     /locus_tag="PKM34_10190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ84001.1"
                     /translation="MAVLAFQKPEKVIMVETDTSKGVFEFRPLEPGFGVTIGNALRRI
                     LLSSLEGFAITSIKIDGVSHEFSTITGVVEDVSEIILNLKKVRFKRQIETENSEKVTV
                     VIGDQDSFKAGDINKFLSVFQVLNPEVEICHLDPSKLTIEINIEKGRGYVPSEENKVM
                     NTDINMIFIDSIHTPIRNVKYKIDNYRVEQKTDYEKLTLEIETDGSIMPKEALKEAAK
                     ILIHHFMLFSDEKITLDTEEKAVAEEFDENSLHIRQLLKTKLVDMDLSVRALNCLKAA
                     DVETLGDLVQFNKNDLLKFRNFGKKSLTELEDLVKTKGLEFGMNVAKYKLDKE"
     gene            1080..1733
                     /gene="rplQ"
                     /locus_tag="PKM34_10195"
     CDS             1080..1733
                     /gene="rplQ"
                     /locus_tag="PKM34_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNQ84002.1"
                     /translation="MRHGKKINHLSRKSAHRKAMLSNMATSLIMHKRITTTLAKAKAL
                     RVYVEPIITRSKEDTTHSRRVVFSYLRSKEAVTELFRDVSAKVADRPGGYTRIIKLGL
                     RLGDNAEMAMIELVDFNLNLLGEKAPAKAKTTRRGRRGGAKKAGAEEVADTVAAPAKP
                     AKAKAKKEEEPVAVAAPAPQSVPAEMPEPEAEPVVVADAELPAEEILPEEAQPADET"
     gene            1903..>2931
                     /gene="eno"
                     /locus_tag="PKM34_10200"
     CDS             1903..>2931
                     /gene="eno"
                     /locus_tag="PKM34_10200"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ84003.1"
                     /translation="MSAIIDIHARQILDSRGNPTIEVDVFTESGVIGRAAIPSGASTG
                     IHEAVELRDGDKEYLGKGVLKAVHNVNNVIKEEIVGYNVTDQGDIDQHMIELDGTPNK
                     AKLGANAILGVSLAAAKAGAMETGQYLYRYVGGVNANTLPIPMMNILNGGSHADNSID
                     FQEFMIMPAGAESFSQALRMGAEVFHTLKGVLKKKGYSTNVGDEGGFAPNLGSNEEAI
                     QVVLEAIEKAGYKPGEDIYIALDPASSEYFLTDEKVYHLKKSSGEKLTPAQMVDYWAE
                     WVSKYPIISIEDGMAEDDWEGWKLMTDKMGSKIQLVGDDLFVTNTKRLQEGISKGVAN
                     SILVKVNQI"
BASE COUNT          906 a          702 c          745 g          578 t
ORIGIN      
        1 gaaaatatcc gggaacagct catcgtcgag ttgtactcca aataatcatc tgaatttttt
       61 cttttaatta taaaaaaacg aacatggcag ttttagcatt ccaaaaaccc gaaaaagtca
      121 tcatggtcga aaccgacacc tcaaaaggtg tgtttgaatt cagacccctg gagcccggtt
      181 tcggtgtgac gatcggaaat gcattaagaa gaatattgct atcgtccctc gaaggttttg
      241 ccattacgtc catcaagatc gatggcgttt cacatgagtt ttctaccatt acaggagtgg
      301 tggaagatgt ttcggaaatt atcctgaacc tcaaaaaggt ccggttcaaa cgtcagattg
      361 agacggaaaa cagcgaaaaa gtaaccgtcg tgatcggtga ccaggactca ttcaaagccg
      421 gcgatattaa caagttctta tcggttttcc aggtgcttaa cccggaagtg gaaatctgcc
      481 acctcgaccc ctctaaactg accatcgaga tcaatatcga gaagggccgc ggttatgttc
      541 ccagcgaaga gaataaagtg atgaacaccg atatcaacat gatatttatc gattctatcc
      601 atactcccat ccggaatgtg aaatacaaga tcgataatta ccgtgtggaa caaaaaaccg
      661 actacgaaaa gctgaccctg gagatcgaaa ccgatggatc catcatgcca aaggaagcgc
      721 tgaaagaagc agctaagatt ctgatccacc atttcatgct cttttcggat gaaaaaatca
      781 ccctggatac ggaagagaaa gccgtagccg aagaattcga tgagaattcg ctgcacatcc
      841 ggcagttgct taagaccaaa ctggtagata tggacctcag cgtcagggca ctcaactgcc
      901 tgaaagctgc agacgtggaa acactcggcg acctggtgca gttcaataaa aacgacctgc
      961 tcaaattcag gaacttcggg aagaagtcgc tgaccgaact ggaagacctg gttaagacca
     1021 aaggacttga gttcggaatg aatgttgcaa aatacaaact tgataaggaa taaatcgcga
     1081 tgagacacgg aaagaaaatc aatcacctga gtagaaaaag cgctcaccgg aaggcaatgc
     1141 tgtcgaatat ggcaacatcg ctcatcatgc ataaacggat cacgacgacc ctggcgaagg
     1201 ccaaagccct cagggtgtac gtcgagccga tcatcacccg ttcaaaggag gatacaaccc
     1261 actccagaag agtggtgttc agctacctgc ggagcaagga agctgtcacg gaacttttca
     1321 gggatgtctc tgccaaagta gccgaccgcc cgggtggtta cacaaggatc atcaaactgg
     1381 gactgaggct gggcgacaat gctgaaatgg cgatgatcga actggttgac ttcaacctca
     1441 acctgctggg tgagaaagct cctgcaaagg ccaagacgac ccgtcgtggc agaaggggcg
     1501 gagcaaagaa agccggcgcc gaagaagtgg ccgataccgt tgcagccccg gccaaaccag
     1561 ccaaagccaa agcaaagaaa gaagaggaac cggtagccgt tgcagccccg gcaccccaga
     1621 gtgtccccgc agagatgccc gaaccggaag cagaaccggt cgtggtagct gatgcggaac
     1681 taccggcaga agaaatactg cccgaggagg cacagccggc tgatgaaacc taaccggaag
     1741 atccggaacc cgaagaagaa acaaaagctt aacattacat gaaatatcaa tggaaaggat
     1801 aaggtggaag cgctttatcc ttttttagtt taaagttttc ataaaacatc tcaatcttat
     1861 agataattaa gaatcaagaa ttaaaaatta agaattaaaa atatgagtgc aataatcgat
     1921 atccatgccc gtcagatcct ggattcacgg ggcaatccaa ccattgaagt ggatgttttt
     1981 accgaatcgg gcgtcatcgg aagggccgcc atcccctcgg gcgcatcgac cgggatccac
     2041 gaagccgtcg aactgcgcga cggtgacaaa gaataccttg gaaaaggtgt cctgaaagct
     2101 gttcacaatg tgaataatgt tataaaagaa gagatcgtcg gatacaatgt gacggaccag
     2161 ggcgatatcg accagcatat gatcgaactc gatggtaccc cgaacaaggc caaactgggc
     2221 gccaacgcca tcctgggcgt ttcgctggcc gctgccaaag caggcgccat ggaaacgggc
     2281 cagtatcttt accggtatgt cggcggggtg aatgccaata cgttgcctat accgatgatg
     2341 aatatcctta acggtggttc acatgccgat aacagcatcg atttccagga gttcatgatc
     2401 atgccggccg gggcggaaag tttcagccag gcgctccgga tgggagcaga ggtattccat
     2461 acgctcaaag gcgtcctcaa gaaaaagggc tattcgacca atgtcggcga cgaaggcggt
     2521 tttgccccga acctgggcag caacgaagaa gccatccagg tagtgctcga agccatcgaa
     2581 aaagcgggat acaaaccggg cgaagatatc tatatcgccc ttgacccggc atcttcggag
     2641 tatttcctga cggatgaaaa agtataccac ctgaagaaat cctccggcga aaagctcact
     2701 cccgcccaaa tggtcgacta ctgggctgaa tgggtcagca agtacccgat catctcgatc
     2761 gaagacggca tggccgaaga cgactgggaa ggctggaagt tgatgaccga caaaatgggg
     2821 agcaaaatcc agctcgtggg cgacgacctg ttcgtgacca acaccaagcg tttgcaggaa
     2881 ggcatcagca aaggcgtggc caacagcatc ctggtaaaag tcaaccagat c
//
LOCUS       DAOQXS010000392         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36445_length_2922_cov_3.293338, whole genome shotgun sequence.
ACCESSION   DAOQXS010000392 DAOQXS010000000
VERSION     DAOQXS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36445_length_2922_cov_3.293338"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..913)
                     /gene="rfbA"
                     /locus_tag="PKM34_10205"
     CDS             complement(47..913)
                     /gene="rfbA"
                     /locus_tag="PKM34_10205"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNQ84004.1"
                     /translation="MKGIILAGGSGTRLYPITMAISKQLMPIYDKPMIYYPLSVLMQA
                     DIREILIISTPYDLPNFEKLLGDGSQIGCRFAYAEQAIPNGLAQAFVIGGKFIGNDKA
                     ALILGDNIFYGTGLHKLLLENNDPDGGVVFAYHVSDPERYGVVEFDDKKQAISIEEKP
                     RQPKSNYAVPGLYFYDNTVVEVAKTMKPSARGEYEITDVNRWYLERGKLKVGILDRGT
                     AWLDTGTFSSLLQASQYVEVIEQRQGLKIGCIEEVAFQMGFIDEKQLVKIAEPLLKSG
                     YGQYLLGLIERK"
     gene            complement(964..>2922)
                     /locus_tag="PKM34_10210"
     CDS             complement(964..>2922)
                     /locus_tag="PKM34_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ84005.1"
                     /translation="EGFLINKETGATLMMVTLNKEEIKNRNRVRLVKDIVDLTDKFGQ
                     NHNIKVHYSGLPYIRIKIAEKLQKEQFMFVLSALALTVLILLAFFRSFKIALFTLIIV
                     LINVAWILGLNVVLGYKLTILTAILPPLLIVIVVENCIFMLTKFHHEFKLHRNKVKAL
                     SRMIMRLGTTNLLTNAATAAGFATFIITGNKILTEFGILASLSILVAYLLTLMLVPII
                     FSYLPAPEQRHIKHLETGPVLRIINTIVNIVSKRRTVIYIITVIIVIVGIAGITQLKS
                     AGKIVDDVARRDPIYKDLAFIEENFKGVMPFEISIDTKKKRGVMKYTNIEKLEQVQKV
                     LAEYPEISKSLSVVDVVKFARQAFYNGDTSYYGLPNTQELNFMIRYLPKMEEGKRTIM
                     NSFLDTNMQVTRITSQLGNIYTYDIERIRDEIRPRIDSIFPAPEYDVLITGTSIVYLE
                     GSKYLMKHLLMSLVLAIILISALMAMLFSSARMIIISLIPNLIPLIMTAGMMGFIGIT
                     IKPSTIIIFSIALGISVDNAIQYLGRYRLFLIYNKWKIRPSVISALKETGFSMIYSSS
                     VLFFGFAIFIFSTFGGTSALGYLISFTLLMALLCNLFILPSFLLTLDKFITTRRFKEP
                     LIDIFDEELDIELEELVVEQQDKKRSEE"
BASE COUNT          720 a          717 c          697 g          788 t
ORIGIN      
        1 attcagcagt tcgtccgaag tatgcatatt aacttttatt ccggtgttat tttctttcga
       61 tcaatccgag cagatactgg ccataaccgc tcttgagcaa aggctctgct atttttacca
      121 gttgtttttc atcaatgaaa cccatctgga atgcaacctc ttcgatacag cctattttca
      181 acccctgccg ctgctcgata acctctacat actgggatgc ctgcagtaat gaactgaagg
      241 tacctgtatc cagccaggcc gttccccggt caaggatccc cactttcagc ttccccctct
      301 ccaggtacca cctgttcaca tccgttatct catattcgcc cctggcgctg ggcttcatgg
      361 tttttgccac ttccaccacc gtattgtcgt aaaaatacag tccgggaacg gcataatttg
      421 atttcggttg cctgggcttt tcttcaatcg atatagcctg ctttttgtcg tcaaactcaa
      481 ccaccccgta tctttcggga tctgatacgt ggtaagcaaa taccacccct ccgtcgggat
      541 cattgttctc gaggagaagc ttgtgcaaac cggttccgta aaagatattg tcgcctaaga
      601 tcagcgctgc cttatcgttg ccgatgaatt tcccgccaat gacaaaagcc tgagccagtc
      661 cgttgggaat agcctgctca gcgtaggcaa aacggcaccc gatctggctt ccgtcgccca
      721 gcaatttttc aaaattcgga agatcatatg gggtagaaat gatcaggatt tcgcggatat
      781 cggcctgcat cagcaccgaa agcgggtaat agatcatcgg cttgtcgtaa atgggcatca
      841 gttgcttgct gatcgccatg gtgattggat aaagccttgt tcctgagcct cctgcgagaa
      901 ttattccctt catctttatg atattatggt tagatgatta ccactgattc tgatggaaaa
      961 tgcctactcc tccgacctct ttttatcttg ttgttccacg acaagttctt ccagctcaat
     1021 atcgagctct tcatcgaaaa tgtcgatgag cggttcctta aatcttcgtg tggtgataaa
     1081 tttgtcgagt gtcaataaga atgatggcag tatgaacagg ttgcaaagca atgccatcag
     1141 caaagtgaac gatatcaggt agccaagggc agaggtgcca ccgaaggtgg agaagatgaa
     1201 gatggcaaag ccgaaaaaca atacgctgga agagtagatc atgctaaagc ccgtctcctt
     1261 cagcgcagat atcaccgaag gtctgatctt ccatttgttg taaatcagga acagcctgta
     1321 acgacccagg tactggatgg cgttatccac cgagatcccc agggcaatgc tgaaaataat
     1381 gatagtagat ggcttgatgg ttatcccgat aaatcccatc atcccggcag tcatgatcaa
     1441 ggggatcagg ttggggatca gggagataat gatcatccgg gccgaactga agagcatggc
     1501 catcagggcc gatatcagga tgatagccag gaccaggctc atcagcaggt gtttcatcag
     1561 gtatttggag ccttccaggt aaacgatgct ggtgccggtg atcaaaacgt cgtactccgg
     1621 ggccgggaaa atggaatcga tccgtggcct gatctcgtcc ctgatccgct cgatgtcgta
     1681 agtgtaaata ttgccaagtt gcgaggtgat ccgggtcacc tgcatgttgg tatccaggaa
     1741 cgagttcatg atcgtcctct ttccttcttc cattttcgga agatagcgga tcataaaatt
     1801 cagttcctgc gtgttcggaa ggccgtaata gctggtgtcg ccgttataga aagcctggcg
     1861 ggcaaatttt accacatcta ctaccgacag cgatttggag atttcggggt attctgccag
     1921 cactttttgc acctgctcca gtttctcgat gttggtatac ttcatcaccc cccttttctt
     1981 ttttgtatcg atggaaatct caaaaggcat caccccttta aaattttctt cgatgaaggc
     2041 gaggtccttg taaatggggt ctctccgggc cacgtcatcc acgatcttcc ctgcggattt
     2101 aagctgggta atgcctgcta tacctacaat aacaataatt acggtgatga tgtaaataac
     2161 cgtcctcctt ttgctgacta tgtttacaat cgtattgatg atcctaagga cgggacccgt
     2221 ttcgagatgt ttgatgtgcc tttgttccgg ggcgggcagg taactgaaga tgatcgggac
     2281 cagcatcagg gtcagcaggt aagccaccag gatgctcagg gaggccagga tgccgaattc
     2341 ggtcagaatt ttattcccgg tgatgataaa cgtggcgaaa cctgccgccg ttgccgcatt
     2401 ggtcaacagg ttggtcgtcc ccagccgcat gatcatccgc gaaagcgcct ttaccttatt
     2461 ccggtgcaac ttaaactcgt ggtgaaactt ggtgagcatg aagatgcagt tctccacgac
     2521 gatcacgatc agcaatggcg gtaaaatagc cgtcaggatc gtcagcttgt accccaggac
     2581 aacattcagt cccaggatcc aggctacatt gatcaggact ataatcaatg tgaacagcgc
     2641 gatcttgaac gaccggaaaa aagccagcag gataagtacg gtcagggcaa gggccgagag
     2701 gacaaacata aactgctctt tctgaagctt ctcggcgatc ttgatccgga tatagggtaa
     2761 tcccgaatag tgtaccttga tgttgtggtt ctgaccgaac ttatcggtta aatccacgat
     2821 atctttgacc agcctgaccc ggttcctgtt tttgatctcc tctttgttca gcgtgaccat
     2881 catcagggtg gccccggttt ctttattgat caggaaacct tc
//
LOCUS       DAOQXS010000393         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36550_length_2917_cov_3.206150, whole genome shotgun sequence.
ACCESSION   DAOQXS010000393 DAOQXS010000000
VERSION     DAOQXS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36550_length_2917_cov_3.206150"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PKM34_10215"
     CDS             <1..1199
                     /locus_tag="PKM34_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNQ84006.1"
                     /translation="ALGSKIYKITQMQGQKSQIPSASWRTKNQTNSKSINLETLAYSL
                     SIDSLTGAISSFIWHPGETPSETSRLHLEKPDGVSSVPSNLVDLSVYSGLNEYLYTSG
                     RDPEKSSTGKLLEIRTIDDGPLVKTFSMELEAPGCESLNVTIRITDALGQVEIINTVR
                     KKKVVEPEAVRFIFPWNVAGGEMTYDLAYGFCRPEKDQLPGANRNYLAMEHWIDISGK
                     QGGVTVVCPDAALFETGKPGMDAIVTGWVDTLPSSQAFLSYAMNNYWETNFAASQEGP
                     CEFSYVVYPHEAFDPALAEQRAIGYRQPLITRKGGGWMKARPSLVQFMNRNLVITSVK
                     PLDNGKALLVSVYNAGMKKEMPRLQNQGKLQVSDPDGINGKPLDPAESIPAGGIKHFI
                     ISAEHW"
     gene            1376..2209
                     /locus_tag="PKM34_10220"
     CDS             1376..2209
                     /locus_tag="PKM34_10220"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84007.1"
                     /translation="MKKLYKTLTLTAIILALTAFTGRSQNAWLNEIHYDDFGTDTVEF
                     IEIVIQDVENFDLSSFEVVLYNGNNGESYDTKSLDEFTVGQVENVFTFFYRVYPTNGI
                     QNGEADGLALVYDGTVIPGQFLSWEGTLTAVDGPASGMTSVDIGVAEAGELEGKSLQL
                     SGESNEYDGFTWQMPSEETMGDVNNDQFFLHFGLKEPGLSHVDFFPNPGNGEGQIFNP
                     FSNNLVLNVFSVDGRMIRQITVKPGENLIRLENLASGIYLINLQEENGRPIGNDKLVI
                     R"
     gene            complement(2213..>2917)
                     /locus_tag="PKM34_10225"
     CDS             complement(2213..>2917)
                     /locus_tag="PKM34_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ84008.1"
                     /translation="SLNIFPNPGLTSETGWSAEIGIRQGFQRGNWYGSLDVAGFWTEY
                     QDMMEFTFGLYLPDSNTLPTLDHIGFKSVNIGVARIAGIDISLNGQVKTGAFLLSYFA
                     GYTYLDPIDHSVDSIENEILKYRYRHSAKGNAEVEYRHYNLGITINYQSFTERIDEAF
                     ETVILGQEFFPGLKDYREANNKGAVVIDIRTGYQLTASSEISLIIKNIFNKEYMGRPG
                     DIQPPRTIALQYVLKI"
BASE COUNT          779 a          715 c          715 g          708 t
ORIGIN      
        1 ccgcccttgg gtcaaaaatc tataaaatca cccaaatgca gggtcaaaaa tcccaaattc
       61 catccgccag ctggcggacc aaaaaccaaa caaattccaa atccataaat ctcgaaacgc
      121 tcgcttattc gctttccatc gattctctga cgggagcaat atcatcgttt atatggcacc
      181 ctggcgagac tccatctgaa acatcgagac tccatctcga aaaaccagat ggcgtctcct
      241 ccgttccctc caatctcgtc gatctttcgg tttactccgg ccttaacgaa tatctctaca
      301 cctcaggaag agatcccgaa aaatcatcca ccggaaaatt actggaaatc aggacgatcg
      361 acgatgggcc gctggtaaaa acgttcagca tggagctgga ggcgccgggc tgcgagagcc
      421 tgaacgttac cattcgcatc acggatgccc tcggccaggt tgaaatcatc aatacggtgc
      481 gtaaaaagaa ggtcgttgag ccggaggcgg tgcgcttcat cttcccgtgg aatgttgccg
      541 gcggcgagat gacatacgac ctggcttatg gcttttgccg gcctgaaaaa gaccagttac
      601 ccggagcgaa ccggaactac ctggccatgg agcactggat cgatatatcg ggcaaacagg
      661 gaggagtgac tgtggtttgt ccggatgcag cattatttga aaccggtaaa ccggggatgg
      721 atgcgattgt gacgggctgg gttgataccc tcccctcctc ccaggctttt ctttcctatg
      781 ccatgaataa ctactgggaa accaacttcg ctgcaagcca ggagggccca tgcgagttca
      841 gctatgtggt ctatccgcat gaagccttcg atccagccct ggccgaacag agagccatcg
      901 gttaccgcca gccgctgatt acccgcaaag ggggcggatg gatgaaagca agaccttcgc
      961 tggttcagtt catgaacagg aacctggtga ttacgtcagt gaaaccgctt gataatggaa
     1021 aagctttgct ggtttcggtt tacaatgcag ggatgaaaaa agagatgccc cggctgcaga
     1081 atcaaggaaa acttcaggtt tcagatcccg acggaattaa cgggaaacct ctggatccgg
     1141 cagaatccat cccggcaggc ggaataaagc attttataat ttcagctgaa cactggtaat
     1201 taagaaaaga aattcgtgtt cattcgctta aattcgcgtt tattcgtgtg ccaatgatcg
     1261 aacgcgaatt aacgcgcatg aaacgaattt gcacgaattt ttgaaactgt aagtaatgtt
     1321 gttttatctt tgagatcggt cttagaaccg gcaagaacat aaaattccaa atcaaatgaa
     1381 aaaactttac aaaaccctga ccctcaccgc catcatcctt gctttaaccg cctttaccgg
     1441 acggtcacaa aatgcctggc tgaatgagat ccactacgat gatttcggga ccgacaccgt
     1501 cgaatttatc gagatcgtga tccaggatgt ggaaaatttc gacctgtcct cattcgaggt
     1561 tgtgctatat aacggcaata acggcgaatc ctacgacaca aaatcgctgg atgagttcac
     1621 cgttggccag gtagagaacg tgtttacatt tttctatcgt gtttacccga ccaacgggat
     1681 ccagaacgga gaagccgacg gactggcgct ggtttatgac ggtaccgtga tcccggggca
     1741 gtttttaagc tgggaaggga cgctcactgc cgttgacggc cctgcatcgg ggatgacttc
     1801 cgtcgatatc ggcgttgcag aagcgggtga actggaagga aaatcgctgc aactctccgg
     1861 ggaatccaac gaatatgacg gtttcacctg gcaaatgcca tctgaagaaa caatgggaga
     1921 tgtgaacaat gatcagttct ttcttcattt tggcctgaag gagccgggtt tgagccacgt
     1981 tgattttttc ccgaatcccg gaaacggtga aggacaaatc ttcaatcctt tctccaataa
     2041 tctggtctta aatgtgttta gtgtagatgg aaggatgata aggcaaataa cggttaaacc
     2101 cggagaaaat ctcattcgcc tggaaaacct tgcctccgga atctacctga tcaatttaca
     2161 ggaggaaaac ggaagaccca tcggtaacga taagctggta atccggtagc ctttagattt
     2221 tcagtacata ttggagggca atggtccggg gtggttgaat atcacccggc ctgcccatgt
     2281 attccttatt aaatatattc ttgattatca ggctgatttc agatgatgcg gtgagttgat
     2341 acccggtgcg gatatcgatc accacagctc ctttattatt tgcttcgcga taatctttca
     2401 ggccgggaaa gaactcctgg ccaaggataa ccgtttcgaa agcctcgtcg atccgttccg
     2461 taaagctctg gtaattgatc gtaattccca ggttataatg cctgtactcc acctccgcat
     2521 tgcctttggc cgaatgcctg taacggtatt tcagtatctc attttctatg gaatcaaccg
     2581 aatggtcaat cggatcaaga taggtgtagc cggcaaaata gctgaggaga aaagccccgg
     2641 ttttcacctg tccgttcagt gagatatcga tccccgcaat gcgggccaca ccgatattga
     2701 ccgatttgaa cccgatatgg tccagggttg gaagggtatt gctgtcgggc aggtacaacc
     2761 cgaaggtaaa ttccatcata tcctggtatt cggtccagaa accggccaca tccagtgacc
     2821 cgtaccagtt tccccgctga aaaccctgcc ggattccgat ctccgcgctc cagcccgttt
     2881 cggaggtcag gccgggattg ggaaaaatat tcaggct
//
LOCUS       DAOQXS010000394         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36595_length_2914_cov_3.194124, whole genome shotgun sequence.
ACCESSION   DAOQXS010000394 DAOQXS010000000
VERSION     DAOQXS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36595_length_2914_cov_3.194124"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2892)
                     /locus_tag="PKM34_10230"
     CDS             complement(<1..2892)
                     /locus_tag="PKM34_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84009.1"
                     /translation="MKKALLLAGFVIMLAGNAALSGQPLSPGLKVLPHDQHLTAVNGS
                     HEDPLYLNYGSPAFNHPSYYYAAVASPATGIFYRQAGLSPDGTKIIAQKSFTEGSFNR
                     TEIVLMDADGTDEIIISAGNSGEGDIYGYMNPFWSDDGNYIGFCEVHNTNANKVMRYQ
                     LSASTTEYIYEPVDNDVSNPDFLGTSITSIVFWAYGPAGGADFFTWDGTTLTNITNTA
                     NYKEYEPVSNPDGTKILFWSGETTAEPVNTTHTLTYSGGVWTKDVGFTPIPDTYWSYW
                     TDNSCTEIGTTVMSTKDIAIYDDEGTFMYDLTGPGYSGGSNQWNFFGSGIKGLNGEML
                     FTSNAGRAEAGRDIVMANPRAKLFVDDNGSDAYPGTVNAPFATIGKGISEALSGTTLT
                     IESGTYNETINGDNKNNLSIIGNATNEPVINGSTHFSPCNDLTLKNLILKNTVSTPIA
                     YIGGINLTMDNVTVDGEDITGHHGISGGHINGDVTVTNCSFLNIKNWVAFDTRSGSGG
                     PTTGALLEDVIFTGNLIDDCQGHINFRGTIGFETDNVTITGNTVQNTGSSTNSFGAAI
                     KVLYATNCIFSGNTIKDVGTSGYNPAGEAPYGAGFMPRNVASLTVTGNTFENNNQAIA
                     IEPRNSVQGGYPDGVLPAGTIANNIFNNNTYGIYIPATLYPNSNPAGLSLENHNKFNN
                     SGEALHFGYSAGMINAEENYWGTEVPDEVELEISGNADYDPWCNADLSVCGFTETSNI
                     YNENQETYFLTVQDAINAASIGDVIEITGTHNVKNLVVNKGLKIAAAAGGAHFSGLDA
                     SRSGTGVVVTDDARITGITFSDYDIAVDIQSGAASIANCTFYNNVIAVKNLAANPVAD
                     ADSCFWGDPTGPSDNSPAGLYNPLGAGDEVTDYIGYQPWYNDIGQTLLAVDIKITNPS
                     CNTLNVYLKSNQAFAGNLTNIQFAVKWDASVYNGIVTPVFKYGSAGEGYI"
BASE COUNT          666 a          705 c          743 g          800 t
ORIGIN      
        1 gatataccct tcaccggcgc tgccatattt aaagacaggg gtgacaatac cattatatac
       61 cgaagcatcc catttgacgg caaactggat attggtcagg tttccggcaa aggcctggtt
      121 gcttttcaga tagacattca gggtattgca ggatggattg gtgattttga tatccaccgc
      181 gagcagggtc tggccgatat cgttgtacca gggctggtag ccgatataat cggtcacctc
      241 atcacccgcc cccagcgggt tgtaaaggcc ggcggggctg ttgtcggatg gccccgtggg
      301 atctccccag aagcaggaat cggcgtcggc aaccgggttg gcggccaggt tcttcacggc
      361 aatcacattg ttgtagaatg tgcaattggc gatcgatgca gcgcccgact ggatgtcgac
      421 ggcaatatcg taatcggaga aggtgatgcc ggtgatccgg gcatcgtcgg taaccacaac
      481 ccctgttccg ctgcgggatg cgtcaagccc gctgaagtga gctccgcctg ccgcggccgc
      541 gatcttcaac cctttgttca ccaccaggtt tttcacattg tgggtgcccg taatttcaat
      601 cacatcgccg atcgaagcgg cattgatggc atcctggaca gtcaggaagt aggtctcctg
      661 gttttcgttg tagatattgg aagtttccgt aaatccgcaa acgctcaggt cggcattgca
      721 ccagggatcg taatccgcat tgccggaaat ttccagttca acctcgtcag ggacttcggt
      781 accccaatag ttttcctctg cgttgatcat tcctgcactg taaccaaaat gcaatgcctc
      841 gccggaattg ttgaatttgt tgtggttttc gagtgataga cctgcaggat tgctgttcgg
      901 atagagggtg gccggaatgt aaattccgta agtattatta ttaaaaatgt tatttgctat
      961 ggttccagcg ggcaatacac catccggata acctccctgg acactgttcc tgggttcaat
     1021 ggcgattgcc tgattgttat tttcaaaggt gttgccggtt acagtcaggc tggccacatt
     1081 gcgcggcatg aaacccgcac cgtacggagc ctcacccgcc gggttatagc cgctggttcc
     1141 gacatctttt atcgtatttc cggaaaatat gcaattagtg gcataaagga cttttatagc
     1201 tgccccgaag ctgttggttg acgacccggt gttctggaca gtgttcccgg taatagtaac
     1261 gttatcggtt tcaaatccga tggtaccacg gaaatttata tgaccctgac aatcatcaat
     1321 aagattgcct gtaaatatta catcctcgag caatgcgcct gtagttgggc caccgcttcc
     1381 gcttcttgta tcaaaagcca cccagttttt tatattcagg aagctgcagt tggtgacagt
     1441 cacatcaccg tttatatgac cgcctgaaat accgtggtgt ccggttatat cctcaccatc
     1501 aacagttaca ttgtccatgg tcaggtttat accgccgatg taagctattg gtgttgatac
     1561 tgtattcttg aggatcaggt tcttaagggt aagatcattg cagggtgaga aatgggtact
     1621 gccattgatg acaggttcgt ttgtggcatt gccgataatg ctgaggttat tcttattatc
     1681 gccattgatg gtctcattat aagtccctga ttcaatggtc agtgtcgttc cggataaggc
     1741 ttcgcttatc cctttgccaa tcgtggcaaa cggcgcattg accgttccgg ggtatgcgtc
     1801 tgaaccgtta tcgtccacaa aaagttttgc tctcgggttg gccatgacaa tatcccttcc
     1861 ggcttccgct ctgccagcat tcgatgtgaa cagcatctcg ccgttcaatc cctttattcc
     1921 cgagccgaaa aagttccact ggttgcttcc cccgctgtac cccgggccgg taagatcata
     1981 catgaaggtg ccctcatcgt cataaatggc aatatccttt gttgacatga ccgttgtacc
     2041 gatctccgtg caactgttat cggtccagta cgaccagtag gtatcgggaa ttggggtaaa
     2101 accgacatct ttggtccaaa ctccgcccga ataggtaagc gtatgggtgg tgttcaccgg
     2161 ttctgcggtg gtttcccccg accagaaaag gatcttcgtg ccatcaggat ttgaaaccgg
     2221 ctcgtactct ttataattgg cggtgttggt gatgttggtc agcgtggtac cgtcccaagt
     2281 gaaaaagtct gcgcctcccg cagggccata agcccagaaa acaatggaag taatggaagt
     2341 tccgagaaaa tcgggattcg aaacatcatt atctacaggc tcgtaaatgt attccgtcgt
     2401 ggaagcagat aattgatagc gcattacctt attagcattg gtattgtgca cttcgcagaa
     2461 gccgatgtaa ttgccgtcat cgctccagaa tgggttcata tacccgtaaa tatcaccttc
     2521 tcctgaattg ccggcggaaa tgatgatttc gtcagtcccg tcggcatcca tcaggacaat
     2581 ttctgttcgg ttaaacgaac cttctgtgaa tgatttctga gctatgattt ttgttccatc
     2641 gggactcagt ccggcttgcc tgtaaaagat gcccgttgcg ggcgaggcca ccgccgcata
     2701 gtagtacgaa ggatggttaa aggccggaga accgtagttc aggtaaaggg gatcttcgtg
     2761 tgatccgtta acagcggtta ggtgctgatc gtgggggagg actttcagcc ccgggctcag
     2821 cggctggcct gataaagcgg cattgcctgc caacattatg acaaaccctg ccaatagtag
     2881 cgcttttttc ataattcggt tttttaatta ttta
//
LOCUS       DAOQXS010000395         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36612_length_2913_cov_3.606718, whole genome shotgun sequence.
ACCESSION   DAOQXS010000395 DAOQXS010000000
VERSION     DAOQXS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36612_length_2913_cov_3.606718"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKM34_10235"
     CDS             complement(<1..437)
                     /locus_tag="PKM34_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ84010.1"
                     /translation="MSHGRFDNPVVASMAGFWDRFRKQELESHFTEKDRFDGKTVLVT
                     GANSGLGFAISTEIARRGGRVIMACRRQIPEAGEKVKAISGSENVEMRHLDLTRIDSI
                     HAFADGLVRDGIKLDVTVLNAGVTPPKARRTQSGLDEMFLVNYL"
     gene            complement(518..1540)
                     /locus_tag="PKM34_10240"
     CDS             complement(518..1540)
                     /locus_tag="PKM34_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNQ84011.1"
                     /translation="MIIRSKAPLRLGLAGGGTDVAPYSDLYGGATLNATINMYAYATI
                     IPKDDGKIVFKSIDKDKYFESPLTDSLEIDGNLNLLKGIYNRIVKDYSHAPLAFELST
                     YVDAPPGSGLGTSSTLVVAVLGAFAEWLRLPLGEYDLARLAYEIERVDLRMAGGKQDQ
                     YAATFGGVNYMEFYKDDKVIVNPLRIKSVYLNELSNNLVLYHTKTSRVSSAIIERQSK
                     NIQDNKLKFIEATHKLKEQAIMMKEAILKGRMDDIGPVLDYGWKFKKQIASGITNHLI
                     DDIYIAAMENGATGGKVSGAGGGGFMVFYCPNNSKYAVIEILKKFGGTSHPYEFTTSG
                     MTAWTI"
     gene            complement(1537..2808)
                     /locus_tag="PKM34_10245"
     CDS             complement(1537..2808)
                     /locus_tag="PKM34_10245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ84012.1"
                     /translation="MDKIVVFGPGPRFKGGIANYNTSLAKALDKLDVAEIYIISWTQQ
                     YPAIIPRDFIDRSSKSDLLEGTKIKVNYITNYNNPFSWNKTVKLIREINPKMVIFQWA
                     IAIQGLPMGYIAKKLKKIKGLEVVFDLHFVIQKEGSKIDKIFTRMGIRHADSYVVHAY
                     KTFDELKKLFPAVGFKLIDPGNRTEGMGKRVVKLFHPVYDMFKPDPDFDVEEEKRRLG
                     LKKNVFLFFGFIRKYKGLHDVIRAFAKIAGQRDDVSLLIVGESFWQTLDTTKLSTRVK
                     NAMFSAAKSVFLRKQDDERNYRPLDLIGELDLKDKVTVVNAFVPNEDVHRYFQVSDAI
                     MLYYLTATPSGVESIGYNFRMPILATRVGHFPETVKDGYNGYLAEPGDLDSMAEVMVK
                     AIEQPISRENVSETSREMSWLNYAKAILNER"
BASE COUNT          718 a          748 c          630 g          817 t
ORIGIN      
        1 agatagttga ccaggaacat ttcatcaagt cccgattggg tcctgcgtgc tttcggcgga
       61 gtgaccccgg cgtttaagac agtgacatcc agtttaatac catccctgac aagtccgtcg
      121 gcaaacgcat ggatgctgtc gatacgggtc aggtcaaggt gccgcatttc aacattttcc
      181 gacccgctga tcgcctttac cttttcaccg gcctcgggga tctgccggcg acaggccatg
      241 atcacacgcc cgcctctccg ggcaatctcg gtactaatgg caaaacccag ccccgaattc
      301 gccccggtaa ccagcactgt tttcccgtca aaacgatcct tttcagtaaa atgtgactcg
      361 agctcctgct ttctgaacct gtcccaaaaa ccggccatcg aagcaactac cggattatca
      421 aatcgtccgt gcgacacaaa agagaatttt aaatttttaa ttcttgattt ttaattgacc
      481 ttgaactttc cactttgaac tttgaacttt gaactgatca gatagtccat gccgtcatgc
      541 cactggtggt aaattcatac gggtggctgg ttcctccgaa ttttttaaga atctcaatga
      601 cagcgtactt tgaattattg ggacagtaaa agaccatgaa accgccacca cctgctcccg
      661 agactttacc tcctgtcgca ccattctcca tggctgctat ataaatatcg tcaatcaggt
      721 gattggtgat gcctgatgca atctgctttt taaatttcca gccgtaatcc agcacagggc
      781 cgatatcatc catcctgccc ttgaggatag cctctttcat catgatggcc tgctccttca
      841 gcttatgagt tgcttcaatg aatttcagct tgttatcctg gatatttttc gattggcgtt
      901 cgataatggc tgatgagacc cggctcgttt tcgtatgata aagaactaaa ttattggata
      961 attcattaag atagacactt ttaattctca gaggattgac aataacttta tcatccttat
     1021 agaattccat ataattcacc ccgccgaatg tcgctgcata ttggtcctgt ttgccgccag
     1081 ccatgcgcaa atcaacccgt tcaatttcat atgccaggcg ggccaggtcg tattctccca
     1141 acggcaatct gagccattct gcaaaagccc ctaaaacagc taccacaagc gtcgaagaag
     1201 ttccaagtcc tgatccggga ggagcatcta cataagttga aagttcaaag gccaaaggtg
     1261 catgcgaata atcctttacg atccggttgt atataccttt cagcagattg aggtttccat
     1321 caatttccag gctgtcggtc aggggcgatt caaagtattt atccttgtcg atggacttaa
     1381 agacgatctt tccgtcgtct tttggaatga tcgtggcata agcgtacata ttgatagtgg
     1441 cattgagtgt ggccccgcca tacagatccg aataaggagc gacatcagtg cctccaccgg
     1501 ccaggcccag cctgagaggt gctttgctac ggattatcat cgctcgttaa ggattgcttt
     1561 tgcataattc aaccagctca tttcacggga tgtctccgac acattctccc tggagatcgg
     1621 ttgttctatc gctttgacca tcacctccgc catcgaatcc aggtcacccg gttcagccag
     1681 gtaaccgtta tagccatcct tgaccgtttc agggaaatgc ccgacccggg tagccaggat
     1741 cggcatccgg aaattatagc cgatcgattc aacgccggac ggggttgctg tgagatagta
     1801 aagcataatg gcatcactca cctggaaata ccggtgaaca tcctcgttgg gaacaaaggc
     1861 attaaccacg gtcactttat cctttagatc cagttcaccg atcaaatcta atggcctgta
     1921 gttgcgttcg tcatcctgtt tacgcagaaa aacggatttg gctgctgaaa acattgcgtt
     1981 cttgacccgg gtggaaagtt ttgttgtatc cagtgtttgc cagaacgatt cgccgacaat
     2041 gagcagggaa acatcatccc tttgcccggc tattttggca aaagcgcgga tgacatcatg
     2101 caagcctttg tatttcctga taaatccaaa aaagaggaaa acattttttt tcaatccaag
     2161 ccgccgcttt tcctcctcca catcaaaatc aggatccggt ttgaacatgt cataaaccgg
     2221 gtggaaaagc ttaaccaccc ttttacccat tccttccgtt cggttccccg ggtctatcag
     2281 cttaaagccg accgccggga acagcttttt caactcatcg aaagtcttat aggcatgcac
     2341 gacataggaa tccgcatgcc gtattcccat ccgggtaaat attttatcga ttttgctccc
     2401 ctctttctgg atgacaaaat gcaagtcaaa gaccacctcc agccccttta tcttcttgag
     2461 ttttttagcg atataaccca tgggaaggcc ctggatggct atggcccact ggaagatgac
     2521 catctttgga ttgatctccc ggattagctt taccgtttta ttccagctga atgggttgtt
     2581 ataattggtg atgtaattga ctttgatctt cgtcccttcc agcaagtccg atttactgct
     2641 cctgtcgatg aaatcccgcg ggatgatggc aggatattgc tgggtccagg aaatgatgta
     2701 tatttcggca acatccagtt tatcgagggc ttttgccagg gaagtgttat aattggcaat
     2761 gccaccttta aaccgggggc cggggccgaa tacgacgatt ttatccattt cggcccggat
     2821 cagagttttc cttctttctt cagtctttcg acggcagcct tgtaaacacg ggtcatccct
     2881 tcccgcaggg tgatcttcgg ttcccatccg aat
//
LOCUS       DAOQXS010000396         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36632_length_2912_cov_3.092405, whole genome shotgun sequence.
ACCESSION   DAOQXS010000396 DAOQXS010000000
VERSION     DAOQXS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36632_length_2912_cov_3.092405"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /locus_tag="PKM34_10250"
     CDS             <1..2182
                     /locus_tag="PKM34_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84013.1"
                     /translation="IQNKSMEDEYKNREADIPEGFVDPVLQKNYSANREISGPVVNMD
                     GIGNLCSCYPPDTDGDVGPNHYFQMINLSFAIWDKQGNLLYGPVDNSTLWDGFIGPWT
                     GTNDGDPIVLYDEMADRWMASQFAVNTNNGSYWQLIAVSTTPDPTGPYHRYAFQMVAF
                     NDYPKLSVWPDGYYATWNMFGSYTRVGVAAFERDAMLAGDPDAQMVYFNQPSGTFSML
                     PADVDGTPPPEDAPCYFGYIRMFSTQNFEVYEFDVNWEFPNMSTFTMVNSMSTAAFNP
                     NLDGIPQPNTGTTLDDLGMMMMFRLQYRNFGSYQTLVANHSVNAGSNRAGIRWYELRK
                     ETDDWYIYQQGTYAPNDGQHRWMGSIAMNGNGDIALGYSLSSTTTYPSIYYVGRRADA
                     PLGEMNLDEVKIMSGTGSQSGINRWGDYSCLSVDPVDDTTFWFTTEYNRNGWKTRIAS
                     FDFGPILPPTIDAGPNDTICEDVLFLADATASYQQSVLWQSSGDGFFPDPNVVDVTYL
                     RGNGDLANGQVTLWATVTGYIEGQTDTDSVTVYFTKTSKAFAGPDTTICDDDVIQLSG
                     SAIFQDSIYWTTEGDGTFDDPGIFTPVYTPGSEDISKGYAWLRLTAYDSLPCDNSHFD
                     RVKITIDECTGISEPEMGKLGLRVVPNPATSRLNFEVTGMEAGTEAVLNLTNQQGAPV
                     FTMKLPATVGSYSNSMNTVNFPRGVYYLKVTGLKEQAIQKVILQ"
     gene            2485..>2912
                     /locus_tag="PKM34_10255"
     CDS             2485..>2912
                     /locus_tag="PKM34_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNQ84014.1"
                     /translation="MNSVLIFAVVSLSAIGLISAVVLYFIAQKFKVVEDPRIGTVEEA
                     LPAANCGGCGFAGCRNFAEALVKKADDEKSLEGLNCPVGGAEVMKKVAAVLELEAVET
                     EPMIAVVRCQGSYKNAPSKVRYEGAATCAFAHNLYAGTSG"
BASE COUNT          731 a          709 c          739 g          733 t
ORIGIN      
        1 catacagaat aaatcaatgg aggacgaata taaaaacagg gaagctgata ttccggaagg
       61 atttgtcgat cctgttttac agaaaaatta cagtgcaaac cgcgaaatca gcgggcctgt
      121 tgtcaatatg gacgggatcg ggaacctctg cagttgttat cctcccgaca ccgacgggga
      181 tgtggggcct aaccattatt tccagatgat caatctttcg tttgccatct gggataaaca
      241 ggggaacctg ctctatggcc cggtcgacaa cagcacgttg tgggatggct ttatcggccc
      301 ctggaccggc acgaacgacg gagatcctat cgttctttac gatgaaatgg ccgaccgctg
      361 gatggcttcg cagtttgccg taaataccaa taacggttcc tactggcagc tgattgccgt
      421 cagtaccaca cccgacccca ccgggcctta tcaccgctat gctttccaga tggtagcctt
      481 taacgattat ccgaaacttt cggtttggcc cgacggctat tacgccacct ggaacatgtt
      541 cggaagctat acccgcgttg gtgttgccgc atttgagcgt gatgccatgc tggccggtga
      601 tcccgatgcc cagatggtct attttaacca gccttccggg accttcagca tgctcccggc
      661 cgacgtcgac ggaaccccgc caccggaaga tgctccctgc tattttggct atatccggat
      721 gttttcaacc cagaactttg aagtgtatga attcgatgtc aactgggaat tcccgaacat
      781 gtcgactttt accatggtca attccatgtc aacagccgct ttcaatccaa atcttgacgg
      841 gatcccgcaa cctaacaccg gtacgacact ggatgacctg ggtatgatga tgatgttccg
      901 cctgcagtac aggaactttg gttcctacca gacgctggtg gccaaccatt cggtgaatgc
      961 gggcagcaac agggccggga tccgatggta tgagctgaga aaagagaccg acgactggta
     1021 catttaccag caggggacct acgccccgaa cgacggccag caccgctgga tgggaagtat
     1081 cgccatgaac ggcaatggcg atattgcgct gggttacagt ctgtcgagca ctactactta
     1141 tccctccatt tattatgtag gaagaagagc tgatgctcca ctgggcgaga tgaacctgga
     1201 tgaggtcaag attatgtcgg gtaccggatc ccagagcggg atcaatcgct ggggcgatta
     1261 cagttgtttg tcggtcgacc cggtcgatga tacgaccttt tggtttacaa cggaatataa
     1321 ccggaatgga tggaaaaccc ggatcgcctc ctttgacttt ggcccgattc tgccaccaac
     1381 tattgacgcc gggcccaatg acaccatttg tgaagatgtc ctgtttcttg ccgatgccac
     1441 tgcatcctac cagcagagcg tcctgtggca gtcgtccgga gatggcttct ttcccgatcc
     1501 gaatgtcgtg gatgtaacct atctcagggg taatggcgac cttgccaacg gacaggtgac
     1561 actctgggct acggtgacag gttatatcga aggccagacc gatacggatt ctgtaaccgt
     1621 ttatttcaca aagacttcca aggcttttgc cggccccgac acgacgattt gcgacgacga
     1681 tgtcatccag ctctccggtt cggccatttt ccaggattcc atctattgga ctaccgaagg
     1741 cgacggtaca ttcgatgatc cggggatttt tacaccggtt tatacgcccg gatccgaaga
     1801 tatatcaaaa ggatacgcct ggttgaggtt aactgcttat gattcattgc cctgtgataa
     1861 ttcgcacttc gacagggtca agatcactat cgatgaatgt accggtattt cagaaccgga
     1921 aatgggtaaa ctggggctca gggtggtacc gaacccggcc acatcgaggc ttaatttcga
     1981 agtaaccggt atggaggccg gtactgaagc cgtgttaaac ctgacaaacc agcaaggcgc
     2041 acctgtgttt accatgaaat tgcccgctac cgtgggtagt tattccaatt caatgaacac
     2101 cgtgaatttc ccgagagggg tttattatct gaaagtaacc ggcttgaaag aacaggctat
     2161 ccaaaaagtc attcttcagt aaacagaaaa taataattgg ataaaccggg caatatgcag
     2221 gatcatccga cttaaaaatg attaagattc aataatttag acggtttcta aatttttaca
     2281 ggaaccccgt gattttcaga agcttcagga acaatatttc tgaagcttct atttttatcc
     2341 gatagaatat taataatcag tattttatac ccaatccgat tttcgatcct tattgttact
     2401 gttttcacaa ttgtttttac atttgctcat cctaaaccgg ccttaagccc ttaatgcgta
     2461 aggcagtatt aaaaagagaa caaagtgaac agtgtattga tttttgccgt tgtgtcgctc
     2521 agtgcgattg gtcttatctc ggcggtagtc ctctatttca tcgcacaaaa gttcaaagtg
     2581 gtcgaagatc cccggatcgg aaccgtagaa gaggctttac ccgctgccaa ttgtggcggt
     2641 tgcggttttg ccggctgccg gaactttgcc gaggcgcttg tgaagaaagc cgatgatgag
     2701 aagtcgcttg aaggattgaa ctgcccggta ggcggagcag aggtcatgaa aaaagtcgcg
     2761 gctgttctgg agctggaagc ggtggaaaca gaacccatga tcgccgttgt gcgttgccag
     2821 ggttcgtata aaaacgcccc gtcgaaggtc agatatgaag gcgccgcaac gtgtgctttt
     2881 gcccataacc tgtatgccgg tacgagcggc tg
//
LOCUS       DAOQXS010000397         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36648_length_2911_cov_3.789216, whole genome shotgun sequence.
ACCESSION   DAOQXS010000397 DAOQXS010000000
VERSION     DAOQXS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36648_length_2911_cov_3.789216"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..>2911)
                     /locus_tag="PKM34_10260"
     CDS             complement(357..>2911)
                     /locus_tag="PKM34_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84015.1"
                     /translation="MLTIPPQDQYGQDWLDQLVIHEFRHSVQYATLDKGFTRAMRILF
                     GQQAVPAVLGLFVPFWYIEGDATVTETAMTRTGRGRSPSFEMRLRAQVIEKGIYSYDK
                     AYNGSYRDFIAGHYEMGYLLVGSGMLKYGGSNLIKPLQKSGTLPFTFVPFSHALYKAT
                     GTGKRKFYRNTLTELGEKWKEEDRNLDTTACSRIREGISRTYTNYTSPIPLPDGLVAA
                     LRSSLDDITRIVLIDKDGRETIACTPGPMIDPVISARGNMICWAELENDPRWALRDYS
                     VIKVHDQQSGRTRQITHKSRYFSPDPDPSAKAIAVVEVDMQNSYYITLVNAADGHAIK
                     RFPVPGNHYPSHPVWSNDGRRIAVILTRSEGKCIAVLDIETAKFEIALPFSYTEISKP
                     TWHEHYILFTGAYTGIDNIFALDLNTGELYQATSARFGATDAAISADGKTLYYASYSS
                     GGYDIVATGFSALSDASHPGDPDSLNPWNPQNNYSFELADGLSEQVGTRFDASAVPDS
                     GYTIRRYCKGLNLFNFHSWAPLYLDIDHLGIQPGVTLMSQNLLASSFATIGWAYDLNT
                     ESGKYMLKYSYEGLYPAFDINLDYGLRHGVIDHTIQENIIYKYHELNTGGWVRVPLNW
                     NIGSWFAGVQPFAGYSYIFRRMLPDQEVEFRKDRFHSVNGRIYAYVQRRMSARDLYPR
                     WAAVTDLNFRHTLFEADTSSSLYSAELSLYLPGLFKHHSLRLYGGYQQRIYNYYPYSD
                     QVSVPRGHSGIALPEIACFSVDYRFPAAYPDWSVGPLVYIKRIKAGIFYDYALQTGTS
                     SGKMYQTVGADLTFDFHLLRHFTPFEAGIRIDYLPQYKQAVFEILYSIGLGN"
BASE COUNT          738 a          707 c          731 g          735 t
ORIGIN      
        1 caacaccagt agcatatcgt agaatttgac ctttgtttga ataataaata cacgctttat
       61 ttccgacctt caacaacttc atagggaaga aaatattagt caaaaataag tcaaatatat
      121 cagataatcg aataaacttt gagtttaatt atatgcattc tctaataatt atgttactga
      181 tattcaatgt gaatttaatt tgcgaataat tctggctttc taatatcaca tctcaccact
      241 ccgacaggtc aattggacaa taggcagttg gacaacttgt cccgagcaga gcgagggata
      301 agacaactgc tttttttatt gccctcattc caaccgggga ggaatctttc ctgccattaa
      361 tttcccagcc cgatgctgta taatatttcg aacacagctt gcttgtattg aggcaggtaa
      421 tctatccgga taccggcttc aaacggggta aaatggcgga ggagatgaaa gtcgaaggtc
      481 agatctgcgc caacggtttg gtacattttg cctgacgaag tcccggtttg caaagcgtaa
      541 tcgtagaata ttccggcttt aatccgttta atgtaaacca atggcccgac gctccagtcg
      601 ggataggctg ccggaaagcg gtaatcgacc gaaaaacagg cgatctcagg caaggcgatg
      661 ccgctatgcc cgcgcggaac cgacacctgg tcgctgtaag gataataatt gtagatccgt
      721 tgctggtacc cgccgtaaag cctcaggctg tgatgcttga aaagacccgg aagataaagg
      781 ctcagctccg ctgaatacaa agagctggag gtgtcggctt caaataaggt atgcctgaaa
      841 ttcagatcgg ttacagcggc ccatcgcggg tagagatcgc gtgccgacat ccgccgttgt
      901 acataagcat aaattcggcc attgaccgaa tgaaaccggt ctttcctaaa ctccacctcc
      961 tggtccggca acatccgacg gaatatatag gaataacccg caaatggctg aaccccggca
     1021 aaccagcttc cgatgttcca gttgagcggc acccgaaccc atccgcctgt attcagctca
     1081 tggtatttgt aaataatatt ttcttgaatg gtatgatcaa taacgccatg ccgtaagcca
     1141 taatccagat tgatgtcaaa tgccgggtaa agaccttcat aactatactt cagcatgtat
     1201 ttgcccgatt ccgtatttaa gtcataagcc cagccgatcg tggcaaagct cgaagccaga
     1261 aggttctgcg acatcagggt gacgcccggc tggatcccca ggtggtcaat atccaggtag
     1321 aggggagccc agctatgaaa gttgaacagg ttcagccctt tacaatagcg ccggatggta
     1381 tagcccgaat ccggtactgc agaagcatca aaccgggttc ccacctgttc cgacagcccg
     1441 tcggccagtt cgaaggagta attgttctgc gggttccagg ggtttaaaga atccggatca
     1501 cccggatggc tcgcgtcaga tagcgctgag aatccggttg cgacaatatc gtaaccgccg
     1561 gatgaatagc tggcgtaata cagcgtcttt ccatctgccg aaatggccgc atctgtggcg
     1621 ccgaaacgcg ccgaggtggc ctggtacaat tcccctgtat tcagatcgag tgcaaaaata
     1681 ttgtcgatgc cggtataggc tccggtgaaa aggatataat gttcatgcca ggttggtttt
     1741 gatatttccg tatatgaaaa cggaagggca atctcaaatt ttgcggtttc aatatccagt
     1801 actgctatgc attttccttc gctgcgggtc aggatcacgg ctattcgccg gccgtcgtta
     1861 ctccacaccg ggtgcgacgg ataatgattg cctggaacgg gaaagcgttt gatggcatgg
     1921 ccatcagccg cattcaccag ggtaatgtag taactgtttt gcatatcaac ttccaccacg
     1981 gcaattgctt tcgcagatgg gtcaggatct ggtgaaaaat agcggctttt gtgggttatc
     2041 tgccgggtcc tgcccgattg ctggtcatgg accttgatta ccgaataatc gcgcagcgcc
     2101 cagcgagggt cgttttccag ttcggcccag cagatcatgt tcccccgggc tgatataacc
     2161 ggatcgatca tcggaccggg agtgcaggca atagtttccc gaccgtcctt atcgatcagc
     2221 acaatgcggg taatatcatc cagcgaagat ctgagagccg caaccaggcc gtcgggcagt
     2281 gggatggggc tggtgtaatt cgtgtacgtc ctggagattc cttccctaat gcgcgaacag
     2341 gccgtggtat caaggtttcg gtcttcttct ttccattttt ccccgagttc ggtcagggta
     2401 tttctgtaaa actttctttt tcctgtaccg gttgctttgt ataacgcatg tgaaaatggg
     2461 acaaacgtaa aaggcagcgt gcccgatttt tgcaaaggtt ttatcaggtt tgagccacca
     2521 tatttcagca tgccgcttcc aaccagcagg tatcccatct cgtaatgacc ggcgatgaaa
     2581 tcacggtatg aaccattata agctttgtca tacgaataga tgcctttttc aatgacctgt
     2641 gccctgagcc tcatttcgaa ggaaggcgac cggcctcttc cggtacgggt catggccgtt
     2701 tcggtcaccg tagcatctcc ttcgatatac caaaagggga caaataagcc caggacagcg
     2761 gggacggcct gctggccgaa caggatacgc atggcgcggg taaatccttt atcgagcgtg
     2821 gcgtattgca cgctgtgcct gaattcatgg atgaccagct ggtcgagcca gtcctgcccg
     2881 tattggtcct gggggggaat ggtcagcata t
//
LOCUS       DAOQXS010000398         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36729_length_2906_cov_3.661873, whole genome shotgun sequence.
ACCESSION   DAOQXS010000398 DAOQXS010000000
VERSION     DAOQXS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36729_length_2906_cov_3.661873"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..228)
                     /locus_tag="PKM34_10265"
     CDS             complement(43..228)
                     /locus_tag="PKM34_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84016.1"
                     /translation="MFQGSLVGQVNFIDDEDDRPFSFYYPFGDVVLFGKYSGTEVKIN
                     NEEHLIMREDDILAVVE"
     gene            142..714
                     /gene="era"
                     /locus_tag="PKM34_10270"
     CDS             142..714
                     /gene="era"
                     /locus_tag="PKM34_10270"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNQ84017.1"
                     /translation="MAERIIKTERPVILIINKIDLTDQAALENKINRWQEIFPTADII
                     PVSALLKANLQSVFRSILEKLPENPPYYDKDELTDKTVRYFVAEKIREKILLNYQKEI
                     PYSVEVAVDQFKESDNLIHIIAYIFIARESQKAILLGHQGSAIKRVGTQARRDLEEWL
                     GKKIFLELSVKVLKDWRDSEKELRKFGYDL"
     gene            742..2046
                     /gene="der"
                     /locus_tag="PKM34_10275"
     CDS             742..2046
                     /gene="der"
                     /locus_tag="PKM34_10275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNQ84018.1"
                     /translation="MPNIVAIVGRPNVGKSTFFNRLVKSRQAIVEETAGVTRDRHYGK
                     SDWNGIEFSIIDTGGYVEGSDDVFEEEIRRQVELAIGESDIIIFMVDVKDGLTEMDKD
                     VAQLLRKSRKKIFLAANKVDNTYRTFDVNEFYALGLGQVYGVSSINGSGTGELLDELV
                     KEFKDTVAAEIPDLPKIAVVGRPNVGKSSLINALLGEERHIVTPIAGTTRDTIYTPYN
                     SFGFEFLLVDTAGLRKKGKVTENIEFYSVMRAIRAIEQSDICLLMINAEEGLESQDLN
                     ILSLIEKNHKGVVILVNKWDLVEKENNTMRDMEAFIKSRTAPFTDLPVVFTSVTNKQR
                     IFKAMEWARRVYENKYRQVSTSKLNELLLPIIQATPPPQTKGKLVKIKYITQLKMHYP
                     TFAFFCNTPQYIKEPYARFVENKLRENFEFTGVPIQIFFRQK"
     gene            2056..2463
                     /locus_tag="PKM34_10280"
     CDS             2056..2463
                     /locus_tag="PKM34_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNQ84019.1"
                     /translation="MPQEKHDSDEVRIDKWLWAVRVYKTRSLATEAIRNGRVKVGGQV
                     VKPSREVHLNDQIDIHLGVYHKTVKVVALLHNRVGAALVPNFLEDLTPEEEYAKLKIQ
                     QEMKPEFRPRGIGRPTKKHRRLIDRLKKTKDFD"
     gene            2591..>2906
                     /locus_tag="PKM34_10285"
     CDS             2591..>2906
                     /locus_tag="PKM34_10285"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ84020.1"
                     /translation="MRKNIISIYTFIILTLNIQLALSQPNEGKFFRIAFYNVENLFDT
                     IDDPMTDDQDFLPGSRIPWTAERYETKLDHLAEVIADLSEGQPVAVFGLCEIENRKVL
                     DDL"
BASE COUNT          816 a          738 c          735 g          617 t
ORIGIN      
        1 tctttactcc catcttcgga gtctcctttc tttaggttaa gcttactcca ccacggcgag
       61 gatgtcgtcc tcgcgcatga tgaggtgctc ttcgttgtta atcttcactt ccgtgccgga
      121 gtactttccg aacaaaacga catcgccgaa aggataataa aaactgaacg gccggtcatc
      181 ctcatcatca ataaaattga cctgaccgac caggctgccc tggaaaataa aatcaaccgg
      241 tggcaggaaa ttttccctac agccgacatc atcccggtat cggccctgct gaaagccaac
      301 ctgcaatcgg tcttccgatc catcctggaa aaactccccg aaaaccctcc ctattacgac
      361 aaagacgagc tgacagataa aacggtccgc tacttcgttg ccgaaaagat cagggaaaag
      421 atcctgctga actatcagaa ggagatcccc tactccgttg aagttgccgt ggatcaattc
      481 aaggagagcg acaacctgat ccatatcatt gcttacatct ttatcgcccg cgaatcgcag
      541 aaggccatac tgctcggcca ccagggcagc gctatcaagc gggtaggcac acaggcccgc
      601 cgggatctcg aagagtggct gggtaagaaa atattcctcg aactgagcgt taaagtgctg
      661 aaagactggc gcgacagtga aaaagaactc cgtaaattcg gatatgacct ctgattgaaa
      721 atcaatcgtt acctttgcag catgccaaat atcgtagcca tcgtaggccg gcccaatgtc
      781 ggcaaatcca cgtttttcaa ccggctggtg aagagccgcc aggccatcgt cgaagaaacc
      841 gccggcgtaa cgcgcgaccg ccattacggt aaatcggact ggaacgggat cgaattctca
      901 atcatcgata ccggaggcta cgtggaagga tcggatgatg tttttgaaga ggagatccgc
      961 cgccaggtgg aactggctat cggcgaatcc gatatcatca tcttcatggt cgacgtcaag
     1021 gatggactga cggagatgga caaagacgtg gcccagctgc tgcgcaaaag caggaaaaag
     1081 atattcctgg ccgccaataa ggtcgataac acataccgga ctttcgatgt caatgagttc
     1141 tacgccctgg gcctcgggca ggtatatggc gtctcctcca tcaacggtag cgggacaggt
     1201 gagctgctcg acgaactggt caaagaattc aaagatacgg tagccgccga aattccggac
     1261 ctgccgaaga tcgcggtagt cggaaggccg aatgtcggga aatcatcgct gatcaacgcg
     1321 ttgctgggag aagagcggca tattgtcacc cctatcgccg gtacaacccg cgacacgatc
     1381 tacactcctt acaacagctt cggattcgaa ttcctgctgg tggatacggc aggccttcgc
     1441 aagaaaggga aagtgacgga gaatatcgag ttctactcgg tgatgcgcgc catccgggca
     1501 attgagcaaa gcgacatttg cctgctgatg atcaatgcgg aagaaggact ggagagccag
     1561 gacctgaata tcctgagcct gatcgagaaa aaccacaaag gggtggtgat cctggtcaac
     1621 aaatgggatc ttgtggagaa ggaaaacaac accatgcgcg acatggaggc cttcatcaag
     1681 tcgcgcacag cgccatttac cgatctgccg gtggtattca cgtcggtgac taacaagcag
     1741 cggattttca aagcgatgga gtgggcgagg agagtttacg aaaacaaata ccgccaggta
     1801 tcgacatcga agcttaacga gctgctcctg ccgatcatcc aggccactcc cccgccgcaa
     1861 accaaaggca aactggtaaa aataaaatat atcacgcagc ttaagatgca ttatccgacc
     1921 tttgcctttt tctgcaatac gccgcaatat atcaaggagc cctatgcccg gtttgtcgaa
     1981 aacaagctga gggaaaactt tgaatttaca ggggtaccga tccagatctt cttcaggcaa
     2041 aagtaaaacc ggaccatgcc ccaggaaaag cacgacagcg atgaagtccg catcgataaa
     2101 tggctctggg cagtcagggt ttataaaacc aggagccttg ccacggaagc catccgaaac
     2161 ggaagggtga aggtcggcgg gcaggtggtg aaaccttccc gcgaggtgca tctgaatgat
     2221 caaattgaca ttcacctggg cgtttaccat aaaacggtaa aagtcgttgc cctgctgcac
     2281 aatcgcgtcg gcgcggccct ggtccccaat tttcttgagg atttaacgcc agaagaagaa
     2341 tatgccaaac tgaagatcca gcaggagatg aagccggaat tcaggcccag aggcattgga
     2401 agaccaacga agaaacaccg ccgcctgatc gatcgcttaa aaaaaacaaa agattttgac
     2461 tgaatgacta tatttacggc ctgttattag ttcgtgctcc aacgactaaa gccgttggtt
     2521 cctattgttt tataaaggta ggaaccgacg gcttcagtcg tcggaagaaa ttcaataaaa
     2581 tgatccaacc atgaggaaaa acattatctc aatctatacc ttcatcattc tcactttgaa
     2641 cattcaacta gctcttagcc agccaaacga aggtaaattc ttccgcatcg ctttctacaa
     2701 cgtggagaac cttttcgata cgatcgacga tccgatgacc gacgaccagg attttctgcc
     2761 cgggtcgagg attccttgga cggctgagcg ctacgagacg aagctggatc acctggccga
     2821 agtgattgct gatttatctg aaggccagcc ggttgctgtt ttcgggctgt gtgagattga
     2881 gaaccggaag gtgctcgacg acctgg
//
LOCUS       DAOQXS010000399         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_36908_length_2897_cov_3.251935, whole genome shotgun sequence.
ACCESSION   DAOQXS010000399 DAOQXS010000000
VERSION     DAOQXS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36908_length_2897_cov_3.251935"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..584
                     /locus_tag="PKM34_10290"
     CDS             3..584
                     /locus_tag="PKM34_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNQ84021.1"
                     /translation="MAHINYWTITLLMAIESSFIPFPSEIVVPPAAYKAAQGELNLFL
                     VIFFSTLGAMIGALFNYYLALTIGRAAIYSFARTRFARMMLVEPKSIQKAEDYFVKNG
                     KISTLIGRLIPAIRQLISIPAGLAKMPLKYFLLYTFIGATGWNIILALLGYFAPKELF
                     ELYYKEISFVMIGLGVLFVMYLVYKGFFQKKSV"
     gene            841..2643
                     /locus_tag="PKM34_10295"
     CDS             841..2643
                     /locus_tag="PKM34_10295"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ84022.1"
                     /translation="MEKTIKLLGALLAFLAFTTFIACDKEEEEPDVIASFTFAVDQTD
                     FMKVQFTNQSTNFSALSWNFGDNSAVSAETNPVHIFPDLGDFVVTLTATSLNGKVTDV
                     FTATVTISDPNAELTKLVGTESKTWKLIRDVSTGRYPLLVFPYNVEDPFTPTTIWWAM
                     GLNNDELANRPCLLNDEWTFYRDGTLEFNNGGDTWAEGGVFKAPDNKCIDDTEAWIGV
                     NDEDISAWGSGTHAFELIAGTPQKLKAIGNGAHIGFFKSATEYEVMKLNPMVQDEVTY
                     DVVKLTDGTTDTLIVQCNYYFEVGDAAFGGSWRYTLVHYDDPNDEPPIPTNNPNPNFS
                     YVVDGLTVTLTNNSLYCDSYLWDFGDGGTSTEAAPTHTYAAEGIYVITLTGTNANGTA
                     SITSEVWISATVLTEEILKGGGTPWKVKVGEKTVFVGSGLGQSNWWAVPAADLQEGGG
                     WACMTDDEFTFLDGGVFTYDGKGVVRNDGYLAEGQGCIEEGTVTGNGAAFLSCTDHSY
                     VFTPATGTTRPIIDLTNGTDRAAFIGFYKGYYGGENGAAPGAEQPLPNGGLTTNKYEI
                     MGYVNDGTKEYILVSVDITADHNGTAAWSVILER"
BASE COUNT          776 a          680 c          678 g          763 t
ORIGIN      
        1 atatggccca tatcaactac tggaccatta ccctgctcat ggctattgaa agctcgttta
       61 tcccgttccc gtcggagatc gtcgtcccgc cggctgctta taaagcagcc cagggtgagc
      121 taaatctgtt ccttgttatt ttcttttcca ccctgggcgc catgatcggg gcgctgttca
      181 actattacct tgcgcttacc atcggaaggg cggcgatata ctcgtttgcc aggacccgct
      241 ttgcccgcat gatgctggta gagccgaaat cgatacaaaa agccgaagac tattttgtta
      301 aaaacggaaa aatatccacc ctgatcggcc gtctgatacc agccatccgg cagcttatct
      361 cgatcccggc cggcctggct aaaatgcctc ttaaatattt tcttttatac acattcatcg
      421 gggcgaccgg ttggaatatc atcctggccc ttttaggcta tttcgcgcca aaagagctgt
      481 ttgaactcta ttacaaagag atcagctttg ttatgatcgg gctgggagtg ctttttgtga
      541 tgtacctggt ttataaagga ttctttcaga agaaaagcgt gtagggataa tttgaaaatt
      601 tgaaaatttg aaaatgcgaa aatttgaaaa tgcgaaaatt tgaaaatgtg aaaatgtgat
      661 attatgaggt atttataggt gtggtcaaat attttcaaaa aaaatccttg aattgcatta
      721 ttgaattatc atttcacata atattgtatt aattttggta taagttagaa aaaactttgt
      781 gatttcgacc aaacataatg aaattcaatc cactaataat taacactaaa ttttacaaaa
      841 atggaaaaaa cgattaaatt gttgggtgcc ttattggcat tcctggcgtt taccacattt
      901 atcgcttgcg ataaggaaga agaagaaccg gatgtaattg ccagctttac ttttgcggtt
      961 gaccagactg actttatgaa agtgcagttc accaaccagt cgaccaactt ctcggcattg
     1021 tcatggaact tcggcgataa ctcagccgtt tctgccgaaa ccaacccggt acacatattc
     1081 ccggatctgg gcgactttgt tgttactttg acggcaacat ccctcaatgg taaggtaacc
     1141 gatgttttca ccgctacagt tacgatcagc gaccccaatg ccgaactgac caaactggtc
     1201 ggcactgagt cgaagacatg gaaactgatc cgcgacgtca gcacgggccg ttacccgttg
     1261 ctcgttttcc cctacaatgt tgaagatcct tttacaccga ccacgatctg gtgggctatg
     1321 ggcctgaaca acgacgaact ggccaaccgc ccctgcctcc tgaacgatga gtggaccttc
     1381 taccgcgacg gtacgctgga attcaacaat ggtggcgata cctgggctga aggtggcgta
     1441 ttcaaagcgc ctgataacaa atgcatcgac gataccgaag cctggatcgg tgtcaatgat
     1501 gaagatatct ctgcctgggg cagtggtaca cacgctttcg aacttatcgc cggaaccccg
     1561 cagaaactga aagcaatcgg caacggcgct catatcgggt tcttcaaatc agctaccgaa
     1621 tatgaggtga tgaaactcaa tcctatggtt caggacgaag tgacatacga tgtcgttaaa
     1681 ctgaccgatg gcacaaccga caccctgatc gtgcagtgca actactactt cgaagtagga
     1741 gatgccgctt tcggtggctc ctggagatat accctggttc attatgacga tccgaacgat
     1801 gagcctccga tcccgaccaa caaccctaac ccgaacttca gctatgtggt ggatggcctg
     1861 acagttaccc tgaccaataa ctcattgtac tgcgattcgt atttatggga tttcggcgat
     1921 ggcggaactt ccactgaagc cgctccgacc catacctacg ctgcggaagg tatttatgtg
     1981 attacgctga ccggaaccaa tgccaatgga acagcttcga taacatccga agtgtggatt
     2041 tcggcaacgg ttctcaccga agaaattctt aaaggcggcg gcaccccatg gaaagtgaaa
     2101 gtcggtgaaa agactgtatt tgtcggtagt ggcctcggcc agagcaactg gtgggcggta
     2161 ccggcggctg acctgcagga aggcggcggc tgggcttgta tgaccgacga tgaatttaca
     2221 ttcttagacg gtggtgtatt cacgtatgac ggtaaaggcg ttgtccgtaa cgacggatac
     2281 ctggcagaag gtcagggatg tattgaagaa ggtacggtta ccggtaatgg agccgctttc
     2341 ctctcctgca ccgaccactc ctatgtattt actcccgcta caggaactac ccggcctatt
     2401 attgacctca ccaatggtac cgacagggct gctttcatcg gattctacaa gggatactac
     2461 ggcggtgaaa atggcgctgc cccgggtgcc gaacaacccc tgccgaatgg tggcctgacg
     2521 accaataagt atgaaatcat gggatatgtc aacgacggaa cgaaagaata tattctggtt
     2581 tctgtcgaca tcaccgctga tcacaacggc accgctgcct ggtcagtgat cctcgaacgt
     2641 tagagaattt atttttggaa ttcttttcag gagcgggcag taatgtcccg ctcctgtttt
     2701 ttttatattt ttatctttta acaactcaat tttaaacatc ttgatattca gaattataag
     2761 taaatatcta ataagttcag tatgaaaaaa gtctacattt tgttattatt cctggccctg
     2821 gcggcacatg catttcccca ggggaggact ataaccggaa gagtaaccga tgaatcggga
     2881 actccgcttc caggggt
//
LOCUS       DAOQXS010000400         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37382_length_2874_cov_4.214970, whole genome shotgun sequence.
ACCESSION   DAOQXS010000400 DAOQXS010000000
VERSION     DAOQXS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37382_length_2874_cov_4.214970"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1322)
                     /locus_tag="PKM34_10300"
     CDS             complement(120..1322)
                     /locus_tag="PKM34_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNQ84023.1"
                     /translation="MIVFLDTVRERKKGTSSVRIQIIYRRYPKFYSTRIQMLPEDWLK
                     MLGLKPRGDLKEKKIIIYEMLRKVLTIIYGMEEFSFEGFEKLYIGEEKTKDDVYAAYD
                     RYIKELKESGRLGSENFYAYSRDSVKKFAGDKRLSFKSINIQFLKKYEAHMLLKGDAL
                     TTVAMYLRCLRFLYNNAIKEGIVRQQDYPFGRGKYEIPQPQNIKKALKLSDIGKIFHY
                     KPESEQESYYRDIWMFSYLCNGINLKDICLLKYSDFQGDHIYFRRAKTINTNRNSKPI
                     DIIVTDEVRQIIDRWGNKPVQPDSFIFPVLTNDLSAEKKHADIAQAVQMTNRYIRRIA
                     KKIGIEANVTTYTARHSFATVLKRSGVNVTYISEALGHSDIKTTENYLDSFEDEQKKE
                     IAKKLTAW"
     gene            2076..2294
                     /locus_tag="PKM34_10305"
     CDS             2076..2294
                     /locus_tag="PKM34_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84024.1"
                     /translation="MLSRRLIVFGFGLFAGIGCYSQSCLPEGIEFVRQGQVDSFPLIY
                     PGCEVIEGNIYIGGLWEQPCKDSTTWIR"
     gene            2252..2521
                     /locus_tag="PKM34_10310"
     CDS             2252..2521
                     /locus_tag="PKM34_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84025.1"
                     /translation="MGTTLQGLDNLDSIGGDLQLGTTSWLADLKGLNSLFFIGGDLSV
                     DYNESLADISALSSLTCLHGDLERYNNDVLTTPSGLKGRLPFSVR"
BASE COUNT          740 a          623 c          642 g          869 t
ORIGIN      
        1 actcctcaat taagccattt tgaaccatat agaataattt tcaagtcaaa gttatgcatt
       61 ttggtaaatt aattttcaaa atgatatgca ttttggtaaa aatattttca gaatgcattt
      121 taccacgctg tcagtttctt ggcaatttct ttcttctgct cgtcttcgaa gctgtccagg
      181 tagttctcgg tggtcttgat gtcggagtgg cccagggctt cgctgatgta agtcacgttg
      241 acacccgacc gtttcaggac cgttgcaaac gaatgcctgg cggtgtaggt ggtgacgttt
      301 gcttcgatgc cgatcttttt ggcaatccgc cggatatacc ggttggtcat ctggactgcc
      361 tgcgctatgt cggcgtgctt cttctccgcc gaaagatcgt tggtcaagac aggaaagatg
      421 aaactgtcag gctggacggg cttgttcccc caccggtcaa tgatctgccg cacttcatcg
      481 gtcacgatga tatcaatggg ttttgaatta cggttggtat tgatcgtctt agccctccgg
      541 aagtaaatgt gatctccctg gaagtctgaa tacttcaaca ggcagatgtc tttcagattg
      601 atgccgttgc acaggtaaga aaacatccag atatcccggt aataggattc ctgctccgat
      661 tcaggcttgt agtggaatat cttcccgata tcggacagct tcaacgcttt cttgatattc
      721 tggggctggg ggatctcata cttgcccctg ccgaaaggat aatcctgctg cctgacaatg
      781 ccctctttga tagcattatt ataaagaaac ctcaggcaac gcaggtacat ggccacggtt
      841 gtcagggcat cgcctttcag gagcatatgc gcctcatact ttttcaggaa ttgtatattg
      901 atgctcttga acgaaagtct tttatccccg gcaaacttct ttacagaatc cctggaatag
      961 gcgtagaagt tttcactgcc cagccgccca ctttctttta attcctttat ataccggtcg
     1021 taagctgcgt aaacatcatc ttttgtcttc tcttcgccga tgtaaagttt ttcaaaccct
     1081 tcaaaagaga attcctccat gccgtaaata atggtcagca ccttacggag catttcatag
     1141 ataatgatct tcttttcctt cagatctccc ctgggcttca atccaagcat tttcagccaa
     1201 tcctcaggca gcatttgaat tctagtggaa tagaattttg gatatcgcct gtaaatgatc
     1261 tggatcctga cgcttgaagt cccctttttt ctttccctga ctgtatctaa aaatacgatc
     1321 acggacactc ctttgttttt aagcaggtca atattttgtt ctggatgggt cagggtcggt
     1381 aatttttcct ttttcttcgg ggtattattt tcccccgaaa ccggattttt atcatttttt
     1441 acaaattgag ggtctcggtt tcccttgatt ttactagctt taccagatga tgccatcgtg
     1501 tgtcctttta cagataagtg atacaaaaaa aataactaac aaaataacta acaaatttaa
     1561 ttaaattatc ggtaataata caaaataaat tataaggaaa tttgttaaaa cattgaatat
     1621 caataataat agaaggataa aaagaggcaa cagatagggt aaaatgtcaa ctggcagtca
     1681 agaggtcatc ggttcgactc cgatatactc cacatcttca ataagttgga tagggtgtcc
     1741 cgactgggtc gggaaggtca tcggttcgac tccggtatac tccacaggat gcacgattta
     1801 tgaaactcga aacacgattg aagaaggaat tcggcaatcg tgtttcgagt ttctggtttc
     1861 tggttccagg ttcgtttgtt cgctggttcg tttgttccct tgttcccttg ttcctcatgc
     1921 ccaatgccat gaaaaatgct aaacgcaaaa cgcaaaacgg aacatgaatc gctttcctga
     1981 gatttcatcc tgtttctgtt ttggcttaat ccgttcgtct tttgattccc ggactatatt
     2041 ttttatattt atatgctcaa atcaaaaagc gattgatgct cagcagaaga ttgatcgtat
     2101 tcggttttgg attatttgcc ggcatcggat gctattccca atcctgcctt ccggaaggga
     2161 tagagtttgt caggcaggga caggtcgact ctttcccgct catttacccg ggttgtgaag
     2221 tcattgaggg aaatatttac atcgggggat tatgggaaca accctgcaag gactcgacaa
     2281 cctggattcg ataggcggtg atcttcagct tgggacgaca tcctggctgg ccgatttgaa
     2341 aggattgaac agtctgtttt ttatcggcgg cgacctgtcg gttgattaca atgaaagcct
     2401 ggcagatata tcggccctgt cgtcgctgac ctgcctccat ggtgacctgg aaagatataa
     2461 caatgatgtt ctgaccacgc cgtcaggctt gaaaggaagg ttacctttca gcgtacgatg
     2521 aaggagaacc atacagggtc aaaatcatct tcaaaaaaga ttttttcaac ggaccgggta
     2581 tttttttcat tttttccgat taatatatat caggaggcct taactggagg catcctcaga
     2641 tatcctgccc ggtgcagagt ggctgcggca tattggcgga tcgttgcgca aatctgcagc
     2701 caatgataaa agtagatcat acgaaaatta tggaaatgtt ttgtgaaacc gatctgttgg
     2761 gactgatccg gcagataaac acaacaaccc gcaattccgg catggaagag aacatgatga
     2821 ttgcggcagg gaaagcggcg gaggcgctgg ctggccggct taattgcagc ccga
//
LOCUS       DAOQXS010000401         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37386_length_2874_cov_3.715857, whole genome shotgun sequence.
ACCESSION   DAOQXS010000401 DAOQXS010000000
VERSION     DAOQXS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37386_length_2874_cov_3.715857"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKM34_10315"
     CDS             <1..990
                     /locus_tag="PKM34_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNQ84026.1"
                     /translation="FFIIVGARFDPASLTEYGTSIYLFLVLLVILFYIVKIIPSFIWI
                     RQFGKRLSLAAGFILAAHLGLVIAASSVGLELGAITPGANASFVIMVVITCLASPLMY
                     GQINRKKAYSDDHTIIIGGSSVGVLLARRLKMLGKSSLIIENSPQRYNEIKSNGLDII
                     AGDGGDPAIYRSANLKPHCYVVVLTANESQDMQICEMLRKELHHERIISIPGNTVIED
                     KMLGLGVHILDARRILASTIENIILRPGAYQSLIETFEEFSVEDITVLNGKIEGKKIM
                     DLPLHKDAMIMLLTRGNEKNVPHGNTYLKMGDVLTIFGTGTGIEEIRDVISRN"
     gene            complement(974..2515)
                     /gene="rny"
                     /locus_tag="PKM34_10320"
     CDS             complement(974..2515)
                     /gene="rny"
                     /locus_tag="PKM34_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNQ84027.1"
                     /translation="MDVLYYLVDGLAALIIGIVIGTFILKRLNSKRFDAMRLEAEEKA
                     EVIKKEKILQAKEKFLELKAEHERAVNERNNAVLINENRIKQKESQISQKFEEIQRKQ
                     AELNTIRENLNNQLEIVSKKQAEYDRIIKEQVEKLETIAGLSAEEAKSQIVETLKEEA
                     KSDAMVYIKDTVEEAKITAQREAKKIVIETIQRTAAEHAIENTVSVFPLENDDIKGRI
                     IGREGRNIRALEALTGVEIIIDDSPEAIILSGFDPVRREIARLSLHKLVTDGRIHPAR
                     IEEVVAKTTKQIEQEVIEVGKRTCIDLGIHNMHNDLVRMIGRMKYRSSYGQNLLAHSI
                     EVAKLSATMAAELGLNPKMAKRAGLLHDIGKVPDNEPDLPHAILGMKMAEKFKEHPEI
                     CNAIGAHHDEIEMESLIAPIVQVCDAISGARPGARREVAEAYIQRLNKLEELALSYPG
                     VVKTYAIQAGRELRVIVGAEKVSDNEASKISYELSRKIQDEMTYPGQIKITVIRETRA
                     VNFAK"
BASE COUNT          650 a          675 c          676 g          873 t
ORIGIN      
        1 ttttttatca ttgtcggggc ccggttcgat cctgccagcc ttacagaata tggaacctcc
       61 atatatcttt tcctggtttt actggtgatc ttgttttata ttgtaaagat tatacccagt
      121 tttatctgga tcagacagtt cggaaaaaga ttatcgctgg cagcgggatt tatcctggct
      181 gcacatctgg gcctggtcat agctgcttct tcggtaggtc tggagcttgg ggcaataacg
      241 ccgggagcta acgcaagttt tgttattatg gtcgtcatca cctgcctggc atcgcccttg
      301 atgtatggac agatcaacag gaagaaggcc tattcggacg accataccat catcatcggt
      361 ggcagcagtg tgggcgtttt attggcccga aggcttaaaa tgctcggaaa atcgtcgttg
      421 atcattgaga atagtccaca aagatataat gagataaagt caaacgggct cgatattatt
      481 gccggtgacg gaggtgaccc tgccatctac cggagtgcta acctgaaacc gcactgctac
      541 gtggttgttc tcacagctaa tgaatcgcag gatatgcaga tatgcgagat gctccggaag
      601 gagttgcatc atgaaaggat aatatccata ccggggaata cggtcataga ggacaagatg
      661 ctgggtttgg gcgttcatat cctggatgcc cgccgtatac tggcttctac cattgaaaat
      721 ataatcctgc ggccgggtgc ttaccagtcg ctcatcgaaa ccttcgagga attctctgtc
      781 gaagatatca ccgttttaaa cggtaaaatt gaggggaaga aaattatgga cctgccattg
      841 cataaagacg ctatgatcat gctcctgacg aggggaaatg aaaagaacgt cccccatggc
      901 aatacttatt tgaagatggg agacgttctg accattttcg gtaccggaac cggtatcgaa
      961 gaaatccgtg atgttatttc gcgaaattaa ctgcgcgggt ttcccggatg acggtaattt
     1021 taatctgacc cggataggtc atttcatcct ggattttcct tgacagctca tacgaaattt
     1081 tactggcttc gttatcggaa actttctcag cgccgacgat aacccgcaat tcccgccccg
     1141 cctggatagc gtaggttttc accacgcccg ggtaggacag ggccagctct tcgagcttat
     1201 tgagccgctg gatgtaagct tccgccactt cgcgcctggc cccgggcctg gcgccggaga
     1261 tggcgtcaca tacctggacg atgggggcga tcagagactc catctcaatt tcgtcgtggt
     1321 gtgcgccgat ggcgttgcaa atttcaggat gctccttgaa cttttctgcc attttcatac
     1381 ccaggatagc atgtggcaga tcgggctcat tatcaggtac ttttccgata tcatgcaaca
     1441 aaccagcgcg tttggccatc ttgggattaa ggcccagttc agcagccata gtagcgctca
     1501 gtttggccac ctcgatcgaa tgggctaaaa ggttctgacc atatgaagaa cggtatttca
     1561 tccgcccgat catcctgaca aggtcgttgt gcatattgtg aatgccaaga tcgatacagg
     1621 ttcgcttacc gacctcgatt acctcctgtt cgatctgctt ggtggttttt gccaccactt
     1681 cttcaatacg ggcaggatgg attcgtccgt cggtgaccag cttatgcagc gataaacggg
     1741 ctatctccct tctgaccgga tcaaaacccg acaggataat ggcttccggc gagtcgtcga
     1801 tgattatttc aacgcctgtc aaagcttcca gagcccggat atttcttcct tcacggccga
     1861 tgatccgccc tttgatgtca tcattttcca gcgggaaaac cgaaacggta ttttcaatgg
     1921 catgttcggc agctgttcgc tggatcgttt caattacgat cttttttgct tcccgctggg
     1981 ccgtgatctt tgcttcttct accgtatctt ttatataaac cattgcgtct gacttggctt
     2041 cttctttaag tgtttcaacg atttgggatt tcgcttcttc cgctgaaagt ccggcaatag
     2101 tttcgagttt ttctacctgc tctttgatta tgcggtcgta ttcggcctgc tttttagata
     2161 caatctcaag ctggttgttg aggttttccc ggatcgtatt cagttctgcc tgttttcgtt
     2221 gtatttcttc gaatttttgt gagatctggg attctttttg ctttattctg ttttcgttga
     2281 tcaggacggc attgtttctt tcattgaccg ctctttcgtg ttcggccttt aattccagga
     2341 acttctcttt ggcttgcagg atcttctctt tttttatgac ttcggctttt tcttccgctt
     2401 ccaggcgcat agcgtcaaaa cgtttggaat tcagcctttt caaaataaaa gttccgatca
     2461 caatcccaat gattagtgct gccagtccat ctactaggta atataataca tccatggttc
     2521 actaacgatt agatttatat gatttatttg ctaatggtca aaaaaatacc cgccatttat
     2581 ccaagcgttt gggataaacc catttcgtaa cgcttagggt tgccccggca ggtcgaactt
     2641 ctgctgtccg ccggctggcg gatcccggtt ttcgccgggt tgcggcctgt tctagctccg
     2701 gtaagtcaaa ccctgatgtt gattgtgttg atttatcaaa ccagaattgg ataaatgcgg
     2761 gtaaatttcg tatggttaat tttcaaagaa ctatagggct tgaattgtca atcaagatgg
     2821 tgtgtgatca attcctccat cttttgaagc cgcgacacca ccatattttc gttc
//
LOCUS       DAOQXS010000402         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37711_length_2857_cov_3.411849, whole genome shotgun sequence.
ACCESSION   DAOQXS010000402 DAOQXS010000000
VERSION     DAOQXS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37711_length_2857_cov_3.411849"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKM34_10325"
     CDS             <1..465
                     /locus_tag="PKM34_10325"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ84028.1"
                     /translation="HLSGDGCWGVENSTAKPGLLGFLPDWAWACSYPHNALQKGVLMP
                     GCVTDYCHVRPHPVYPTSLYESVFSFIVFSLLWSVRKKVNVTGSLIVIFILLNASFRF
                     LIEFIRINPKYNFLGCHFSQAQLISILLFFITLTGYYYFRRRSQKQKKQTSF"
     gene            573..2078
                     /gene="accC"
                     /locus_tag="PKM34_10330"
     CDS             573..2078
                     /gene="accC"
                     /locus_tag="PKM34_10330"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNQ84029.1"
                     /translation="MIRKILVANRGEIAVRVMRSCREMDILTVAVYSDADGGAMHVRY
                     ADEAYHIGPAPSSESYLKIDKIIEVAKRCGAEAIHPGYGFLSENAAFSDRCKEEGIIF
                     IGPSGDSIRSMGDKITARKNMIAAGVPVVPGTTEPISDEDKALSVIREIGLPVMIKAS
                     AGGGGKGMRLVKEENEILNAIRAARSEARSAFGDDSVYIEKYIESPHHIEFQILADNH
                     GNTIHLFERECSVQRRHQKVVEETPSPLLTPSVREEMGKFAVAAARAVNYSGAGTIEF
                     IVDESLNYYFLEMNTRLQVEHPITERVVGVDLVKEQIRIANNEVMALKQEELKQRGHA
                     IECRIYAEDPDRNFMPSPGVIKHITEPHGLGVRCDGYVYEGYEIPLYYDPMISKLICW
                     GKTRDEAIARMKRALYEYKITGVKTSIRLLVRIMDTPDFKEGKYNTHFIEKNRDLLFT
                     MPPINPRVEDVAIMTVFMDYLAKVENFQTKVVTNKTKSNWKACGRRKNMSE"
     gene            2191..2700
                     /locus_tag="PKM34_10335"
     CDS             2191..2700
                     /locus_tag="PKM34_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNQ84030.1"
                     /translation="MRSLEIQIGDKISKVELLGRKGSELKMKVDDRIYEPDIIMVERG
                     VYSILLDGRSYNVELIETTGPKQYQVNTLFYTFDAEVVDAETKYLKSRRKIDGLEDNI
                     ISSPMPGKVVKILVRPGEEVKAGETVVIVSAMKMESEYKVKKDRLIKEIRVKEGDTVS
                     ANQPLIIIE"
BASE COUNT          837 a          666 c          710 g          644 t
ORIGIN      
        1 cacctttcag gcgacggatg ctggggagtg gaaaattcaa cggcaaaacc cgggttgctt
       61 ggctttttgc ccgattgggc ctgggcttgc agctacccgc ataatgcact ccaaaaaggg
      121 gttctgatgc ccggttgcgt gacagactat tgccatgtgc gcccacaccc cgtttacccg
      181 acctcattat acgaatcggt gttttctttc attgttttct ccctgttatg gtcggttcgt
      241 aaaaaagtaa atgtgaccgg tagcctgatc gtgattttca tcctgctgaa tgcctcattc
      301 cgctttttaa ttgaatttat ccgcatcaac ccgaaatata attttttagg ttgtcatttt
      361 tcccaggcgc aactaatttc aattctttta ttttttatta cactgacggg ctattattat
      421 ttccggcgaa gatctcaaaa acagaaaaaa caaacatcat tttaaaaatt attatcttta
      481 caatcctttt ctaatcgccg caagatgata atggactaac aatgagacac atataatcat
      541 tttgcttatt tgaaattaat ttaaataaag atatgatcag aaaaattctc gttgccaaca
      601 ggggtgaaat tgcggtaagg gtcatgcggt cgtgccgtga aatggatatc ctgacggtag
      661 cggtttattc ggacgccgac gggggcgcca tgcatgtcag gtatgccgat gaagcttacc
      721 acatcgggcc ggcgccatcc agcgaaagct acctgaaaat cgacaagatc attgaagtgg
      781 caaagcggtg cggcgccgag gcgatccacc cgggatacgg attcctgtcg gaaaatgcag
      841 cattttcaga ccgttgcaag gaggaaggga tcattttcat cggcccttcg ggcgattcga
      901 tccggtcgat gggcgataag ataacggcga gaaaaaacat gatcgcagcc ggggtgcccg
      961 ttgttccggg gacgaccgaa ccgatcagcg acgaagacaa agccctttcc gtgatccggg
     1021 aaatcgggct gccggtgatg atcaaagcat cggccggcgg cggaggaaaa gggatgcggc
     1081 tggtcaagga agagaacgaa atactgaatg ccatccgggc ggcccgctcc gaagcccggt
     1141 cggccttcgg cgacgactcg gtttacattg aaaaatacat cgaatcgccc caccatatcg
     1201 agttccagat cctggccgat aaccacggca acaccattca cctcttcgag cgggaatgtt
     1261 cggtgcagcg ccgccaccag aaggtggtcg aagagactcc atctcctttg ctgacccctt
     1321 cggtaaggga agagatggga aaatttgccg tcgcggctgc cagagcggtg aattactcgg
     1381 gcgccggcac gatcgagttc atagtcgacg aaagcctgaa ttactacttt ctcgagatga
     1441 acacccgcct gcaggttgag cacccgatca ccgaacgcgt cgtcggtgtc gacctggtga
     1501 aagaacagat caggatagcc aataatgagg tcatggcact gaaacaggag gagctgaaac
     1561 agcgcggcca cgccatcgaa tgccggatct atgcggagga tcccgaccgt aacttcatgc
     1621 ccagtccagg ggtgatcaag catatcaccg aaccccacgg cctgggtgtc aggtgtgatg
     1681 ggtatgttta cgaaggctac gaaatcccgc tatactacga cccgatgatc tccaaactca
     1741 tctgctgggg aaaaacccgt gatgaagcga tcgccaggat gaaaagggcg ctttatgaat
     1801 acaagatcac gggggttaaa acatccatcc ggcttctggt caggatcatg gacacgcccg
     1861 atttcaagga aggtaaatac aatacgcact ttatcgaaaa gaacagggac ctgttattta
     1921 ccatgccccc gatcaatccg cgcgtagaag atgtggccat tatgacggta tttatggatt
     1981 acctggccaa ggtcgaaaac ttccagacca aagtcgtgac aaacaaaaca aaaagcaact
     2041 ggaaagcctg cgggcgaagg aagaacatga gcgaataaca attcaagttc aaagttcaaa
     2101 gttcaaagtt caaagttcaa agagaaatat tcatcaggcc tcaaattaaa aattaagaat
     2161 taaaaattaa gaattcaaca ttatctaatt atgcgttcac tcgaaattca gatcggagat
     2221 aaaatttcaa aagttgagct gttgggccga aaaggcagcg agcttaagat gaaagtggat
     2281 gaccggatct acgaacccga tatcattatg gttgagcggg gtgtttattc tattctgctg
     2341 gatggccggt cgtacaatgt ggagttgatc gagaccaccg ggccgaagca ataccaggtc
     2401 aatacgctgt tttacacctt cgacgccgaa gtggtggatg cggagaccaa gtacctgaaa
     2461 agccgccgga agattgacgg gctggaagac aatatcatct cttcgccgat gcccggaaaa
     2521 gtagtgaaga tccttgtcag gccgggagaa gaggtcaaag cgggtgaaac cgtggtcatc
     2581 gtttcggcca tgaagatgga gagtgaatat aaagtaaaga aagacaggct gatcaaggag
     2641 atcagggtga aggagggcga tacggtgagc gcgaatcaac cactgatcat cattgagtaa
     2701 attccatccg ccagatggca gacaaaattc caaattccaa actccacatt ccaaataaaa
     2761 tccaaactcc aaattccaaa ctgacgacaa atcctaaatc ctgatttcta aatcctaaac
     2821 aaatcctaat taccaaaatc caaatgatcc aaacatg
//
LOCUS       DAOQXS010000403         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37869_length_2849_cov_3.735147, whole genome shotgun sequence.
ACCESSION   DAOQXS010000403 DAOQXS010000000
VERSION     DAOQXS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37869_length_2849_cov_3.735147"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1093
                     /locus_tag="PKM34_10340"
     CDS             173..1093
                     /locus_tag="PKM34_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84031.1"
                     /translation="MVRSGWRTIRNYFIILLYFLAIPLQGQNLTDLLPNPAELPGWTL
                     QSQTVMNGDELYMLINGGADIYHEYGFSRVVSALYSDPGQNVIRAEIYEMTDDGAAYG
                     VYSINHLKASWTGEFGKQSTVTDDFIAFWKSRYYVILSWKSKKNGGKPLLGQLAAIID
                     GKVSTSGDIPGILGLCSAQASSSNKAYLRGNIGLSNFYYIDYRNPFAFKEAMVFSRED
                     YHVILLEYTNSTESFENMQSARQSLSENKRFTDLTNTYQGFSCKDNKGNRIVVRQENR
                     YLILLVGLSPDAPLPAKMEEISLEIDNLNH"
     gene            1106..1927
                     /locus_tag="PKM34_10345"
     CDS             1106..1927
                     /locus_tag="PKM34_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNQ84032.1"
                     /translation="MCGRYVLVQKVELIEKRFNVEAPQGFDFQPNYNIVPGSYAPVIT
                     NENPRQLELFRFGLTPFWSKKPLLLINARAEGDHNPDDNPEYTGARGIITKPSFRKPI
                     RSQRCLIPADCFIEGTTKEKLSRPYVLYLREGERPFAFAGVWDTWANPDTGEIIRSFA
                     IITTVANELLRKIPHHRSPVILPRSLEKAWLSDDLPLSDVTGMLRPYPAELMNAYPVD
                     PAIKSPRAAGPQLLKPVGQRLTAEFDVRKSQDVNLQGMGMNKRLKDQQDYLEFEL"
     gene            complement(1967..2812)
                     /gene="nfo"
                     /locus_tag="PKM34_10350"
     CDS             complement(1967..2812)
                     /gene="nfo"
                     /locus_tag="PKM34_10350"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ84033.1"
                     /translation="MKYVGAHVSAAGGVENAPLNAYKIGAKAFAFFTKNQKQWKTAPL
                     SEKNIREFKANCLKYNYLPESILPHDGYLINPGSPDKEMLEKSREALLDEMQRCEQLG
                     LKMLNFHPGAHLGKISEEDCIKIIAESINIVLSKTSDVTAVIENTAGQGSAIGHRFEH
                     LRAIIDQVDDKSRVGVCLDTCHAYAAGYDLKNPEGYDKAFDEFGKIIGFNYLRGFHLN
                     DARKGLASRVDRHESLGKGDIGEDLFIRIMNDPRFDNMPIILETPNEEIWPEEIRWLY
                     ETVGI"
BASE COUNT          662 a          649 c          768 g          770 t
ORIGIN      
        1 gacatgcctc gatgctgctc cggccgatat cctgactgta tacatggcgt atgaacatgg
       61 gctcggggag atgaacctgg agaaactggc tgttgccgaa tttgaagttt gatttgaatt
      121 ttccggaaag aattttattg agatggagtc tctgagattt agaatggttt ttatggtgag
      181 aagtggttgg cgcacaatcc ggaattattt cattattcta ctttattttc ttgccatacc
      241 tcttcagggt cagaatctga cagatctgtt gcccaatccg gcagaattac ctggctggac
      301 tttgcagtcg cagacggtca tgaacgggga tgagctatat atgctgatca acggcggggc
      361 cgatatttat catgagtatg ggttcagccg cgtggtatcg gccctttatt ccgatcccgg
      421 ccagaatgtt atccgtgcgg agatttatga gatgacggat gacggtgccg cgtacggtgt
      481 ttacagcatt aatcacctaa aggcatcatg gaccggggag tttggcaaac aatcgacggt
      541 tactgatgat ttcatcgcct tttggaagag ccggtattat gtgatcctgt catggaaatc
      601 gaaaaagaat ggcggaaagc cattactggg acaattagct gctataattg acggaaaggt
      661 ttcaacctcc ggcgacatac ccgggatact gggactttgc tcagcgcagg cttcatcttc
      721 aaacaaagct tatctgcgcg gaaatatcgg cttgtctaat ttctattaca ttgattaccg
      781 caatcccttt gcttttaagg aggctatggt attttccagg gaggattacc atgtcattct
      841 gcttgaatat accaattcca ctgaatcgtt tgaaaatatg cagtcggcgc ggcagagtct
      901 ttctgaaaac aagcgattta ccgatctgac caatacctac cagggatttt cgtgtaagga
      961 taacaaagga aacaggatag tagtgagaca ggagaacagg tatctgatcc tgcttgtggg
     1021 attgtcgccg gatgcgccgc taccggcgaa gatggaagag atttcgctgg aaatcgacaa
     1081 tttaaaccat taacgaaagc ggattatgtg tggccggtat gttttggttc aaaaggtgga
     1141 attaatcgaa aagcggttta acgtcgaagc gccgcaagga tttgactttc agcctaacta
     1201 caatatcgta ccgggaagct atgcaccggt gatcacgaat gaaaacccca ggcaactgga
     1261 gctttttcgt ttcgggctga cacctttctg gtcgaagaaa ccgcttctgc tgatcaacgc
     1321 cagggcagag ggggatcata atcctgacga caatcctgag tataccggcg cccgggggat
     1381 catcaccaaa ccctctttcc gcaaaccgat ccgttcgcag cgatgcctta tccccgccga
     1441 ttgcttcatt gaagggacga caaaagaaaa gctctcgagg ccttacgtgt tatacctgag
     1501 agaaggggag agaccatttg catttgccgg cgtctgggat acctgggcta atccggatac
     1561 gggtgagatc atccgctcct ttgccatcat tacgaccgta gccaatgaac tcctccgtaa
     1621 gataccgcac caccgttcgc cggtcatttt gcccaggtcg cttgagaaag cctggttgag
     1681 cgatgatctg ccgctgtcgg atgttaccgg tatgctgcgc ccttacccgg cggaattgat
     1741 gaatgcctat ccggtcgacc cggcgatcaa gagcccccgt gcagcagggc cccaattgct
     1801 caaaccggtt ggccagcggc tcactgcgga attcgatgtt agaaagtcgc aggatgttaa
     1861 cctgcagggg atgggaatga ataaacgatt gaaagatcag caggattacc tggagtttga
     1921 gctatagtca gctataagca gtcttttcct ttttcccttt tcccttctag attcccaccg
     1981 tttcatagag ccagcggatc tcttcaggcc agatctcttc gttgggggtt tcgaggatga
     2041 tgggcatgtt atcgaagcgc ggatcgttca tgatccggat aaacaggtct tcgccgatat
     2101 cccctttgcc gaggctttcg tgccggtcga cgcgggaagc gagtcctttg cgggcgtcgt
     2161 tcaggtggaa tcctctcagg tagttgaacc cgatgatctt accgaactca tcgaacgctt
     2221 tgtcgtatcc ttccgggttt ttcaggtcat agccggcggc ataggcatgg caggtgtcga
     2281 ggcaaactcc tacacggctt ttatcgtcga cctggtcgat gatggcccgg aggtgttcga
     2341 accggtgacc gatcgctgaa ccttggccgg ctgtattttc gatcactgct gtcacgtcac
     2401 tggttttcga caggacaata ttgatggatt cggcgatgat tttgatgcaa tcctcttcgc
     2461 tgatctttcc caggtgtgcg ccgggatgga agttgagcat tttgaggccg agctgttcgc
     2521 agcgttgcat ttcatccaga agggcttcgc gtgatttttc gagcatctct ttgtcggggc
     2581 ttccgggatt gatgaggtag ccgtcgtgcg gcagtatgct ttcaggtaga taattatatt
     2641 tcaggcagtt agccttgaac tcccggatgt ttttttctga tagtggtgcg gttttccact
     2701 gtttttggtt tttggtgaag aaggcaaagg ctttcgcacc tattttataa gcgttgagcg
     2761 gggcattttc gacgccacct gctgcgctga catgagcacc gacgtatttc ataggggttg
     2821 gaattttggc aaaggtagga aaaaagggg
//
LOCUS       DAOQXS010000404         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37874_length_2849_cov_3.247316, whole genome shotgun sequence.
ACCESSION   DAOQXS010000404 DAOQXS010000000
VERSION     DAOQXS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37874_length_2849_cov_3.247316"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKM34_10355"
     CDS             complement(<1..318)
                     /locus_tag="PKM34_10355"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNQ84034.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDVLTPGYDIIARFQGGPNAGHTIE
                     FNHKKFILHTIPSGIFYPEKTNLVGNGVILDPFIFLKEINNLADHGIDATANLVISKR
                     THLI"
     gene            complement(342..815)
                     /locus_tag="PKM34_10360"
     CDS             complement(342..815)
                     /locus_tag="PKM34_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ84035.1"
                     /translation="MKEREQSDSFETVKKIFTEYLEQRGHRKTPERFTILKEIYNTDG
                     HFDIETLYIRMKNNKYRVSRATLYNTIELLLDCSLVTKHQFGNNCAQFERSYKFKKHD
                     HFIDTTTGKVIEFSDPRIDDIRRFVEDTLKVRVSHHSLTFYGSSLDEHKEPALTH"
     gene            complement(977..>2849)
                     /locus_tag="PKM34_10365"
     CDS             complement(977..>2849)
                     /locus_tag="PKM34_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNQ84036.1"
                     /translation="QPATSQQAENFRKILLTLARDVRVILIKLADRLHNMRKLEALPV
                     DKQLKIASETIYLFAPLAHRLGLFSIKSELEDLALRATEPEIYHTLQKKLQETRTDRI
                     RFTNKFIYPIKKGLRERGVKFQIFTREKSIYSIWQKMKNKEIPFEEVYDLFAVRIVLD
                     VPIPMEKSECWQVYSIITDHYRPNHSRLRDWISIPKANGYEALHTTVMSHAGKWVEVQ
                     IRTRRMDEIAERGYAAHWKYKDTIDSETGVEEWLKKVKDMLREEEPDAVDFVNDFKLN
                     LFADEIYVYTPRGEVRTLPSGSSVLDFAYVVHTEIGNHCIGAKVNQVLVSRKHILKNG
                     DQVEVLSSNSTLPEADWLQYAMTAKARANIKQWVREQQRKMTMLGKEKLGVLFQQYNL
                     PDDKASVQRFMSFHNIIKEDDLYYMIASGDLTADDFKNFRVEGEKKNFFSYLSRPFTR
                     QKPGKNRTLTETIVEKLKEKPESLLLSDDITQIKYVLSRCCSPIPGDDVVGFLNENGS
                     IQIHRTNCPDTIDLLSKYGKRIVKAKWKNKESIGFLTGIKIQGVDKKGFIRKVTEVIT
                     EKHNINIRSFHLNTSEGMTEGTIMLYLHDTQTLQKLIDNLMKIKEIIKVTRIDRFNE"
BASE COUNT          638 a          717 c          653 g          841 t
ORIGIN      
        1 tatcagatgt gtccttttcg aaataaccaa attggccgtc gcatcgatac catgatcggc
       61 caggttgttg atctctttga ggaaaatgaa aggatcgagg ataacgccgt tgccaaccag
      121 attcgtcttc tccggataaa agatacccga agggatggta tgcaggatga attttttatg
      181 gttgaactcg atggtgtggc cggcattcgg accgccctgg aaccgcgcaa tgatgtcgta
      241 tccgggtgtc aggacatcca caatcttccc cttgccttcg tcaccccact gaaggccaag
      301 aataacatct actttcattt caattggtct ggtgtataaa tctaatgggt aagtgcgggt
      361 tctttgtgtt catccaggct cgacccgtaa aaagtgagcg aatggtgcga aaccctgact
      421 ttcagtgtat cttccacaaa ccggcggata tcgtcgatcc ggggatcgct gaattcgatc
      481 acttttcctg tggttgtatc gataaaatgg tcgtgcttct tgaacttgta cgaccgttcg
      541 aactgtgcac agttgttgcc gaactggtgt ttggtgacca ggctgcagtc gagcagcagt
      601 tcgatggtat tgtataatgt tgcgcggctg acccggtact tgttattctt catccggata
      661 tagagggttt cgatatcgaa gtgcccgtcg gtattataaa tttccttcag gatggtgaac
      721 cgttccggcg ttttcctgtg gcctcgctgc tccaggtatt ccgtgaaaat cttcttaacc
      781 gtctcgaatg aatcgctttg ttctctttct ttcatacaca ttacagaaaa gtgcataaaa
      841 gtagaaaaat tattattact atttggtata aataaaaata attttttaac atgtttcccc
      901 ctccgtcgta atgacgccaa ccctcaccct ggcaacggaa ctgccgactg cctactgccg
      961 actgcctact gccctcctac tcattgaacc tgtcgatcct cgtcactttg atgatctcct
     1021 ttatcttcat caggttatca atgagcttct gtagtgtctg agtatcatga agatagagca
     1081 tgatcgtccc ttccgtcatc ccttcggaag tattcaggtg gaaagaacgg atattgatgt
     1141 tgtgcttttc ggtgatcact tcggtcacct tgcggatgaa tcccttttta tccactcctt
     1201 gtatctttat ccctgtcaaa aagccaatcg actccttatt tttccatttc gctttcacga
     1261 tccgcttgcc atatttcgaa agcaggtcga tcgtatccgg gcagttggtc cggtggatct
     1321 ggatcgagcc gttctcgttc aggaaaccga ccacatcatc accggggata ggactgcagc
     1381 agcgcgacaa aacatatttg atctgggtga tatcatcact cagaagcaat gattcgggct
     1441 tctccttcag tttctcgaca attgtttcgg tcagggtcct gtttttcccc ggtttctgcc
     1501 gggtgaacgg ccggctgaga tagctgaaga agttcttctt ctccccttcc accctgaaat
     1561 ttttaaaatc atcggcggta aggtcgccgc tggcaatcat ataatacagg tcatcttcct
     1621 taatgatgtt gtggaagctc atgaaccgct ggaccgaagc tttatcatcg ggcaggttat
     1681 attgttggaa taacacgccc agtttttctt tcccgagcat ggtcattttc ctctgctgtt
     1741 ccctgaccca ttgctttata ttggcccgcg ctttagccgt catggcgtat tggagccagt
     1801 cggcctccgg cagggtactg ttggaagata agacctccac ctggtcgccg ttcttcagga
     1861 tatgcttacg ggaaaccagt acctggttga cttttgcccc gatgcaatga ttcccgatct
     1921 cggtgtggac tacgtaggca aaatcgagca cggaagaacc cgaaggcaat gtccttactt
     1981 caccccttgg ggtataaaca tagatttcat ccgcaaaaag gttcagttta aaatcattga
     2041 cgaagtctac tgcatccggt tcttcttcac gcagcatgtc ctttaccttt ttcaaccatt
     2101 cctccacgcc tgtctcgctg tcgatggtat ccttgtattt ccagtgggca gcatatcctc
     2161 tttccgcgat ctcgtccatc ctccgggtcc gaatctgcac ctccacccat ttaccggcat
     2221 ggctcatgac ggttgtatga agggcctcat aaccgttggc tttgggaatg gagatccagt
     2281 cgcgcaggcg gctgtgattc ggccggtaat gatcggtaat gatcgaatag acctgccagc
     2341 actccgattt ttccatggga atgggaacat ccagtacaat cctgacagca aataaatcgt
     2401 acacctcctc gaaagggatc tctttgttct tcattttctg ccagatggag taaatgctct
     2461 tctcccgggt aaagatctgg aacttgacgc ccctttccct gagccctttt tttatcgggt
     2521 agatgaattt attggtaaaa cggatgcggt ctgtccgcgt ttcctggagt tttttctgaa
     2581 gcgtatgata aatctcaggt tcggtagccc tcaaagccag gtcttccaat tcgcttttta
     2641 tcgaaaacag tcctaaccgg tgggccagcg gcgcgaacag atatatcgtt tcggaagcga
     2701 ttttcaactg tttgtcaacc ggaagggcct ccagctttct catgttatgc aaccggtcgg
     2761 ccagtttgat caaaattacc ctgacatccc gggccagcgt cagcaggatc tttcggaaat
     2821 tctccgcctg ttgcgacgtc gccggttga
//
LOCUS       DAOQXS010000405         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_37961_length_2844_cov_3.596271, whole genome shotgun sequence.
ACCESSION   DAOQXS010000405 DAOQXS010000000
VERSION     DAOQXS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37961_length_2844_cov_3.596271"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..408
                     /gene="crcB"
                     /locus_tag="PKM34_10370"
     CDS             31..408
                     /gene="crcB"
                     /locus_tag="PKM34_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNQ84037.1"
                     /translation="MLKMFLITGAGGFLGSGLRYLVQRIIAIYLPVTFPFATLFINVF
                     GSLLIGIIYALSDKTSMLNPEMRIFLAIGVCGGFTTFSTFSLDTYSLVKDGAYLFVSL
                     YIFASVILSIAAVFAGIWIVKSL"
     gene            411..773
                     /locus_tag="PKM34_10375"
     CDS             411..773
                     /locus_tag="PKM34_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNQ84038.1"
                     /translation="MKSLTGSATLLRIYVGELDKYHHQPLYEAIVLFAKRQGLAGATV
                     LRGLMSYGANSMIHTSKLFALSEDLPIIIEIVDEEDKIQDFIQKLQPYFESAKYGGLV
                     TLEEIRVIYYQNSKTTQD"
     gene            791..2500
                     /locus_tag="PKM34_10380"
     CDS             791..2500
                     /locus_tag="PKM34_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNQ84039.1"
                     /translation="MKIPLNQFEQYIDETILKRGLSYFKKGLVEDPEELSPGFFEAIV
                     EGSESYRVTVSIKNEIITDHSCTCPYDLGPVCKHIAALLFALQAEKLGINKQNKITAA
                     KNSTEMTRKKGRKTVIEQVDEILTKLPPNHIAEFIREQCIQNPAFRRLFIARFITLFE
                     GESKVVYAQQVKAILNSAKGRHGFIEWNRAGAVGKAVYEMLVNARKQLEVKNYQTVML
                     ISCAVLEEMFKALQFADDSDGDIGGGVSEAVEILFLVSEEDLPEENRKWLFDYTIQAI
                     KKRLYNGWDWDLDMFALAANLVKGEKEASALLELIESMAFSEFMEEHVQEIRVKILRK
                     SGREDEADAFEAKHLNNPIFREQAIKKAIAANDLDKAGQIAHEGIKQDKKDKPGLVGD
                     WVDWLLKIAQKRNDRQMIITHARYLFDMANRDHKYYYKILKETVDQAEWPSFTDELIR
                     QLSDHRRWGDPYLLAWICIEEEKWETLLKVCDRGFTLGGVLEYEKHLAKHYPEELAAI
                     YKRKILELLDRSTGRNHYQEAARFIRRMKKIGATAQAEQLVGEIKQKYPQRRALLEEL
                     EKV"
     gene            2559..>2844
                     /locus_tag="PKM34_10385"
     CDS             2559..>2844
                     /locus_tag="PKM34_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84040.1"
                     /translation="MKLDFTKGVFVQVGGELGKHNSLPIDNFIQFAQNLQNTILNIAK
                     HDLPLDQPIDPDNFRLEITDFKKGSAVPQFAFTHRYQETLGETLSIHREKV"
BASE COUNT          852 a          564 c          678 g          750 t
ORIGIN      
        1 gactcctgtt cttgaactaa acgaattcct atgttgaaga tgtttttaat cacaggggca
       61 ggaggttttt taggaagcgg gctcaggtac ctggtacagc gaattatcgc catctatctc
      121 ccggtcacct tccctttcgc caccttgttc atcaatgttt tcggttccct gctcatcggg
      181 ataatatatg ccctgagcga caaaacaagc atgttaaatc ccgaaatgcg gatttttctg
      241 gctataggtg tttgcggcgg attcaccact ttttcgacct tttcgctcga cacttacagc
      301 ctggtcaaag acggtgccta tttatttgta tccctctata tttttgccag tgtaatttta
      361 agtattgccg ccgttttcgc cggaatctgg atcgttaaat cgttgtagtc atgaaatcac
      421 ttaccggaag tgccacttta ttgcgcattt atgttggaga actcgataag tatcatcatc
      481 agccgcttta cgaagccatt gtcttgtttg ccaaacgaca ggggctggcc ggcgccaccg
      541 tattgcgcgg gcttatgtcg tacggggcga acagcatgat ccatacttca aaattatttg
      601 ccctttcgga agatctaccc atcatcatcg aaatcgtgga tgaagaggat aaaatacagg
      661 atttcatcca aaagcttcaa ccctatttcg aaagtgcaaa gtacggcggg ctggtgacac
      721 tggaagagat cagggtgatt tattatcaga acagtaaaac aactcaggat taaacataaa
      781 agcgaacctc atgaaaatcc ctttaaacca gtttgagcag tatatcgatg aaactatcct
      841 gaaaagaggt ttaagttatt tcaagaaagg cctggtggag gatcctgagg aattatcgcc
      901 gggattcttc gaagctatag ttgaaggatc tgaatcatac agggtaacgg tttctattaa
      961 aaacgaaatc attacggacc attcttgtac ctgcccatac gatctgggtc cggtgtgcaa
     1021 gcatattgca gcgctcttat ttgctttaca ggctgaaaag cttggaatta acaagcaaaa
     1081 taaaataacc gctgctaaaa attcaacaga aatgaccaga aagaaaggtc ggaaaacggt
     1141 cattgagcag gtagatgaga tcctgaccaa actgcctcca aatcatattg cggaatttat
     1201 acgggaacag tgcatacaga atcctgcatt tcgcaggctg tttattgccc ggtttattac
     1261 cctgtttgag ggtgaatcga aagtggttta cgctcaacag gtaaaggcga tacttaattc
     1321 ggcaaaaggg cgtcatggat ttatagaatg gaacagggcc ggagcggtag gaaaagctgt
     1381 atatgaaatg ctggtcaatg cccggaaaca gttggaagtg aaaaactatc agacggtcat
     1441 gctcatttcc tgcgctgttc tggaggaaat gttcaaagcg cttcaatttg cggatgacag
     1501 cgacggcgat atcggaggag gtgtcagcga agctgtggag atcctgtttc ttgtttcaga
     1561 ggaagacctg cctgaagaga accggaaatg gctgttcgat tatacgatac aggcaataaa
     1621 gaagaggctt tacaatggat gggactggga cctggatatg tttgcccttg ccgccaattt
     1681 ggtgaaagga gaaaaagaag cttctgcttt gctggaattg atcgaatcga tggcattttc
     1741 tgaattcatg gaagaacatg tccaggagat cagggtgaaa atactcagga aaagtggtcg
     1801 tgaggatgaa gcagatgcat ttgaagcaaa gcatttaaat aatccgattt tcagggaaca
     1861 agctatcaag aaggctattg cagccaatga tcttgacaaa gccgggcaaa ttgctcatga
     1921 aggcataaag caggacaaga aagacaaacc ggggcttgtc ggtgattggg tggactggct
     1981 tttaaagatc gctcagaaga ggaacgaccg gcaaatgatc attacccatg ccaggtatct
     2041 atttgacatg gctaatcgcg atcacaaata ttactacaaa atattaaagg aaaccgttga
     2101 tcaggctgag tggccctcat ttacagatga actgatccgt cagctttctg atcacagacg
     2161 ttggggagat ccctatctgt tagcctggat atgtatagaa gaagaaaagt gggaaacttt
     2221 acttaaggtt tgtgaccgtg gtttcaccct cggcggggtc ctggaatatg aaaaacatct
     2281 tgcaaaacac taccctgaag agcttgccgc aatttataag aggaaaatac tggaacttct
     2341 ggaccggagt accggaagga accattacca ggaagccgca cgctttatcc ggagaatgaa
     2401 aaaaataggt gcaacggcgc aggcagaaca actggttggc gaaataaagc aaaaatatcc
     2461 acagcgcagg gcgctgttgg aggagctgga gaaggtgtga ggaattttcc cagataattt
     2521 caaggaatta tttaatttta ctactgatta atcccaccat gaaattagat ttcacgaaag
     2581 gtgtttttgt gcaggtcggt ggtgaactgg gaaagcataa cagtttgcca atcgataatt
     2641 ttattcagtt tgcccaaaat cttcaaaata ctattctcaa tatagctaag catgatctac
     2701 ctttggatca accgattgat ccagacaact tcaggcttga aattactgat tttaaaaaag
     2761 gaagtgctgt acctcagttt gcttttactc atcgttatca ggaaaccctt ggcgaaaccc
     2821 tgtcaatcca tcgcgaaaag gttt
//
LOCUS       DAOQXS010000406         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_38167_length_2834_cov_3.840590, whole genome shotgun sequence.
ACCESSION   DAOQXS010000406 DAOQXS010000000
VERSION     DAOQXS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38167_length_2834_cov_3.840590"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKM34_10390"
     CDS             complement(<1..678)
                     /locus_tag="PKM34_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84041.1"
                     /translation="MQNFELLQPGKFYHVYNCGINGEDLFRNNADFERFLRLFEKYIM
                     PVCETYAWVLMKNHFHFLVRIKENIGYKYSMNDFRDDKEKFNELKWETTNIVLTCRHP
                     EDLKVIKPTPHLHFSHLFNAYAKYFNIKYKRHGSLFERPFHRKKIDNKKYFKNVIVYI
                     HQNPVHHGFCDYPDEWGWSSYLTVISIKPTQLDREEVIGWFDNVGNFKDCHNNIIDRL
                     EFERYLGL"
     gene            914..1858
                     /locus_tag="PKM34_10395"
     CDS             914..1858
                     /locus_tag="PKM34_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ84042.1"
                     /translation="MPELSLVISVYNEEENILSLSEKIREALTGLDYEVIFVDDGSRD
                     KTVPMILSIVDPRVILLKFKKNYGQSSALSAGIDFASGDYIVTLDGDLQNDPADIPMM
                     LKVAREGDWDLVASNRKNRKDGMLIRKIPSRIANRIIRKTTGVKMKDLGCALKVFKKD
                     TAKNLGLYGELHRFIAVMAALDGATITQVDVNHFPRIHGQSKYGLSRTFKVISDLILL
                     LFIKRYLQKPMHFFGKWGLLILLPGVAINIYMLILKILGHDIWGRPLLILGIMLVVAG
                     FQMITSGIIAELVMRTYYESQQKKPYRVSNIFKGGKQA"
     gene            1842..2729
                     /locus_tag="PKM34_10400"
     CDS             1842..2729
                     /locus_tag="PKM34_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ84043.1"
                     /translation="MGSKLKRLLKTLLKFAVTAAALYFVIRKIELKEVLALYSQSNLV
                     YITLALIFFALSKLVSAYRLNIYFRTIDIHLKEKTNIELYLLGMFYNLFLPGGIGGDG
                     YKVYLLQKKYQAGTKKIIGAVLSDRISGMVALVVLALLGISILNPEVIGRWSLDVSRG
                     LLFSSVVIAIVTVFLAYYLFTKIAYPYFLKINILTFTYGFLVQLLQVACAWFLLLALG
                     EHENHLSYLVIFLVSSAVAVLPVSIGGMGVRELTFLYGSQILNVDMNVAVGISFLFYL
                     ITATVSLTGVWYIFRPVKL"
BASE COUNT          771 a          648 c          594 g          821 t
ORIGIN      
        1 caaccccagg tatctttcaa actccaatct gtcaattata ttattatggc aatctttaaa
       61 attccctaca ttatcaaacc atcctataac ttcttcccta tctaattgtg tcggtttaat
      121 ggagattaca gtcaggtacg atgaccatcc ccattcatca gggtaatcac aaaaaccatg
      181 atgaacagga ttctggtgaa tatagactat aacattttta aagtatttct tattatctat
      241 tttctttcga tggaatggtc tttcgaaaag gcttccgtgt cgcttatatt taatattgaa
      301 atatttcgca taagcattga acagatgtga gaaatgaaga tggggtgttg gttttatcac
      361 tttcaggtcc tcaggatgcc gacaggttaa tacaatattg gttgtttccc atttcaattc
      421 attaaatttt tccttatcat ctctaaaatc attcattgaa tatttataac caatattctc
      481 cttaatcctt accaaaaaat gaaaatgatt cttcatcaac acccatgcgt atgtctcgca
      541 gacaggcata atatacttct caaataagcg gagaaatctt tcaaaatcgg cattgttcct
      601 gaaaagatct tctccgttaa ttccacaatt ataaacatgg tagaatttac ctggttgaag
      661 taattcaaag ttttgcatgt ttccttcctt tctgctgttg taaccccgtc aggtccttcg
      721 gacgccgtca ggtttgaaac taattaccag gttggaataa tatggaaggt ctgtttagat
      781 tatatccagg cagctaagat aagaaaaatg tcttcaatga aaaatcattt ttcgaaatcc
      841 ttcaaacctg acagcgtccg aaggacccgt cagcgtttga aaaataaata tctttgcacc
      901 aaaaccagac accatgcctg aactctccct ggtcatttca gtttataatg aggaagaaaa
      961 tatcctttcc ctgtcggaga agatcaggga agcgctgacc ggtttggatt atgaggtgat
     1021 ctttgtggat gatggctcca gggacaaaac cgtgcccatg atcctgtcga tcgtcgatcc
     1081 ccgcgtcatc cttctgaaat tcaagaagaa ttacggccag agttcagcct tgtcggccgg
     1141 gatcgatttc gcctccggcg attatatcgt caccctggat ggcgacctcc agaacgatcc
     1201 ggccgatatc ccgatgatgc tcaaagtggc cagagagggc gactgggacc tggtggccag
     1261 caaccggaaa aaccgcaagg acggcatgct gatccggaaa ataccgtcgc gtatcgccaa
     1321 ccggatcatc cgtaaaacaa ccggggtcaa aatgaaagac ctgggttgcg cactgaaagt
     1381 ctttaaaaag gatacagcca aaaacctcgg attgtatggc gaactgcacc gctttatcgc
     1441 cgtcatggcg gccctcgacg gcgccacgat cacacaggtc gacgtgaacc acttcccgcg
     1501 gatacacggc cagtcaaaat acggcctttc aaggactttc aaagtgatca gcgacctgat
     1561 cctgttgctt tttatcaagc ggtacctgca gaaaccgatg cacttcttcg gaaaatgggg
     1621 attgctgatc ctgttgcccg gcgtggcaat caacatctat atgctgatcc tgaagattct
     1681 gggacacgac atctggggcc gcccattgct gatcctggga atcatgctgg tagtcgccgg
     1741 tttccagatg atcaccagcg gcatcattgc cgaactggta atgcggacct actacgaatc
     1801 acaacaaaaa aagccgtacc gcgtcagcaa tattttcaaa ggtgggaagc aagcttaaac
     1861 gactgctgaa aacactgctc aaattcgctg tgactgcggc ggcattgtac tttgttatcc
     1921 gaaaaataga actgaaagag gtcctggccc tgtacagcca gtccaacctg gtatatatta
     1981 ccctggctct cattttcttt gctctttcga aactcgtatc ggcctacagg ctgaatattt
     2041 atttccgcac catcgatatt cacctgaaag aaaaaacgaa tattgaacta taccttctcg
     2101 ggatgtttta caacctgttc ctcccgggtg gaatcggcgg agacggatac aaggtctatc
     2161 tccttcagaa aaaataccag gccggaacga aaaaaattat cggcgcggtt ctgtccgacc
     2221 gcatttccgg catggtcgcg ctggttgtgc tggctttgct gggtatatcc attctgaatc
     2281 ctgaagtcat tggtcgatgg tcgctggacg taagtcgcgg attattattt agttccgtgg
     2341 ttattgccat tgtcacagtg ttccttgctt attacttgtt taccaagatc gcatatccct
     2401 atttcctgaa gatcaatatc ttaacattta catatggctt cctggttcaa ttgttacaag
     2461 ttgcctgtgc ctggttttta ctcctggcat tgggtgaaca tgaaaaccac ctctcttacc
     2521 tggttatttt cctggtttca tcggccgtgg ctgttttgcc ggtatctatc ggtggtatgg
     2581 gggtcaggga actgactttt ctttacgggt cgcagatact gaatgtcgat atgaatgtag
     2641 ccgtcgggat cagcttcctg ttctacctga tcacggcaac tgtttcactg acaggagttt
     2701 ggtatatatt tcgaccggtt aaactctgaa attccaaatt ccaaacacca agcaccaaac
     2761 aaattagaaa ttcaaaaaat caaagttaag cgtttttaat tttaaatctt ggtgtttgaa
     2821 tttgtttggg attt
//
LOCUS       DAOQXS010000407         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_38307_length_2828_cov_3.212405, whole genome shotgun sequence.
ACCESSION   DAOQXS010000407 DAOQXS010000000
VERSION     DAOQXS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38307_length_2828_cov_3.212405"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PKM34_10405"
     CDS             complement(<1..117)
                     /locus_tag="PKM34_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNQ84044.1"
                     /translation="MKIICIGRNYVEHARELNSPLPEKPVFFLKPDTALVIRN"
     gene            complement(286..1890)
                     /locus_tag="PKM34_10410"
     CDS             complement(286..1890)
                     /locus_tag="PKM34_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNQ84045.1"
                     /translation="MKFPALDQIPERFRLAEPVFQRDYLINGEILEWNGDLQEVFSPV
                     CIEDSNGQASPILIGSYPVMTARESLVALEAACNAFDHGRGEWPTLSVEGRIAHLEDF
                     AIRMIEQRDPVINLLMWEIGKSYDDSVKEFDRTVEYIRDTIEALKELDRNSSRFQIQQ
                     GIIGQVRRSPLGVVLCMGPFNYPLNETFTTLIPALIMGNTVIFKPPKYGVLLHHPLLK
                     AFRDSFPPGVVNTVYGEGPVVVAPLMESGKVDVLAFIGTSRVASILKKQHPRPHRLRS
                     VLGLEAKNPAIVLANADIDLAVSECLLGSLSYNGQRCTALKIIFVHESIAGEFNNKMV
                     AGLEKMKQGMPWEDNVRITPLPEAGKTEYLKGLIGDAVSKGAKIINPGGGMVSKTFFS
                     PALVYPVNREMKLWREEQFGPVVPIVPFREIEEPLEYVTSSDYGQQVSIFGKDAADIA
                     HLIDPLVNQVCRLNINSQCQRGPDTFPFTGRKDSAEGTLSVSDALRVFSIRSLVAAKS
                     NTLNKQIITTILKEGSSNFLSTDFIF"
     gene            complement(2000..2803)
                     /locus_tag="PKM34_10415"
     CDS             complement(2000..2803)
                     /locus_tag="PKM34_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ84046.1"
                     /translation="MELSLTRPLAVFDLETTGTNISTDRIVEICIVKAMPDGSRQIYS
                     KRINPVIPISKESISVHGITDLDVKDCPRFNDLAVELDQFLTDCDLSGYNAIKFDIPL
                     LVEEFLRAGIDFSLMDRNIVDVQNIFHKMEPRTLKAAYRFYCGKELEMAHSAEADTLA
                     TLEVLQAQLDKYEGVDYNDNEGLISKPVRNDVKALHDFSFYTRNVDLVGHIVFNDKQE
                     EVLNFGKYKGQSVEQVFKKEPSYFDWMMKSQFPLSTKRVIQMIWKRMGK"
BASE COUNT          726 a          671 c          633 g          798 t
ORIGIN      
        1 gtttcgtatc accaacgctg tatccggttt cagaaagaaa acaggctttt ccggtaaagg
       61 gctgttcagc tccctggcat gttcaacata attacggcct atgcagatga ttttcatggt
      121 cgttagtcat tagtagttag tcatcagtag tagtcatcag tagtttatca ttagccttaa
      181 atcataagtc ataagttatt aatattcatc cattaagaat taagaattaa gaattaagaa
      241 ttataaatta taaattcaac attactaatt aaacattaaa cattattaaa agataaagtc
      301 tgttgataaa aagttcgatg atccttcttt caggattgtc gtaatgatct gcttgttcag
      361 cgtattgctt ttggcagcaa ccaggcttcg gatggaaaat acccgcaacg catcggacac
      421 tgaaagggtg ccttcggctg aatctttgcg gccggtaaac gggaaggtgt cgggcccgcg
      481 ctggcactgg ctgttgatgt tgagcctgca aacctggttg accagcggat cgatcagatg
      541 ggcaatatcg gcggcatcct ttccgaagat gctcacctgc tggccataat cggatgaggt
      601 gacatactcc agcggctctt caatttccct gaaaggtaca atggggacga ccggcccgaa
      661 ctgttcttcg cgccagagct tcatttcgcg gtttaccggg taaaccaggg ctggggagaa
      721 gaaggtcttg ctaaccatac cgccgccggg attgattatt tttgctcctt ttgatacagc
      781 atcaccgata agtcccttca agtattccgt cttgcctgct tcaggcaatg gggtgatcct
      841 gacattatcc tcccagggca ttccctgttt cattttttcc agtccggcaa ccatcttgtt
      901 attgaattcc ccggctatgg attcatggac aaaaattatt ttcagggcgg tacacctttg
      961 tccattgtaa gataaacttc ccagcaggca ctcgctgact gccaggtcga tatctgcgtt
     1021 ggccagtacg atcgccggat ttttcgcctc aagtccgagt acgctgcgaa gcctgtgtgg
     1081 cctcggatgc tgctttttca ggatggatgc aacgcggctg gttccgataa atgccagcac
     1141 atccaccttt cccgattcca tcagcggggc taccactacc ggcccttcgc cataaaccgt
     1201 gttgacgacc cctggaggga atgaatcgcg gaaagctttt agcaatgggt gatggagcag
     1261 caccccgtat ttcggaggtt tgaaaattac ggtgttgccc atgatcaggg cagggatcag
     1321 tgtagtaaat gtctcgttca gaggataatt gaaaggtccc atgcagagca cgactccaag
     1381 cggcgatcgc ctgacctggc caatgatccc ctgttgtatt tgaaaacggg aagaattccg
     1441 gtcaagctct tttaaggctt caatagtatc acggatgtat tctaccgtac ggtcgaattc
     1501 tttgaccgaa tcgtcgtatg attttccaat ctcccacatc agcagattaa taacaggatc
     1561 acgctgttca atcatccgga tagcaaagtc ttccaggtgt gcaattctcc cttcaactga
     1621 taaggtcggc cattcgcccc ggccatggtc aaaagcatta caggctgctt cgagggcaac
     1681 taaagactcc cgggccgtca tgaccggata ggagccgatc aaaatgggag aggcttgccc
     1741 attgctatcc tcaatacaaa cgggtgaaaa gacctcctgg agatcgccgt tccactccag
     1801 gatctccccg ttaatcaaat aatctctttg aaaaaccggc tctgctaacc ggaatctttc
     1861 cgggatctgg tctaaagcag gaaacttcat caatgtttat ttaataaaaa gttaatgtca
     1921 tcaaaaccct caggaacact ttacaatttt cagtttacag tttacaattt gcactttgaa
     1981 ctttgaactt tggactaact tactttccca ttctcttcca gatcatctgt atcacccttt
     2041 tggtcgacaa ggggaactgg cttttcatca tccagtcgaa atatgagggt tcctttttaa
     2101 aaacctgctc aaccgactgt cctttgtatt ttccaaaatt cagtacctct tcctgtttat
     2161 cattgaatac tatgtgtcct accaggtcta cattccgtgt gtaaaacgaa aaatcgtgca
     2221 gcgctttaac gtcatttctt accggtttgc ttatcagtcc ttcgttatcg ttgtaatcca
     2281 ctccttcgta cttatcgagc tgggcttgca ggacctccag tgttgcgagt gtatcagcct
     2341 cggccgaatg agccatctca agctccttac cgcaatagaa gcggtatgcg gcttttagcg
     2401 tccggggttc cattttatgg aagatattct ggacatctac aatgttacgg tccataagag
     2461 aaaagtcgat gccggccctc aaaaactcct caaccaggag cggaatatcg aatttaatgg
     2521 cgttgtaccc tgaaaggtcg caatctgtta aaaactgatc cagttccaca gccaggtcat
     2581 tgaaccgggg gcagtcttta acatccaggt cggttattcc atgaacggat atagattctt
     2641 ttgaaatggg aataacggga ttgatccgtt tgctgtagat ctgccggctg ccatcgggca
     2701 tcgccttcac aatacaaatc tcaacaattc ggtcggtgga tatgttggtt ccggttgttt
     2761 caaggtcaaa tactgccagc gggcgggtga gagataattc catcaattta aaatttaaag
     2821 tttaaggg
//
LOCUS       DAOQXS010000408         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_38637_length_2813_cov_3.245830, whole genome shotgun sequence.
ACCESSION   DAOQXS010000408 DAOQXS010000000
VERSION     DAOQXS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38637_length_2813_cov_3.245830"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKM34_10420"
     CDS             complement(<1..554)
                     /locus_tag="PKM34_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84047.1"
                     /translation="MKQFIAFIFGSICFLFFTVNVNSQWAFNGTHIYNTNAGNVGIVT
                     NAPGTLLDVAKSMTEPTIRVYNLGGNGGATYQMKDALSGADWKFKATNLGGFKIRDHA
                     LSLDVMVIEQNSAANCIYIQQGGNVGIGTNSPLEKLDVNGAVRIGNTANSNAGAIKWD
                     GTNFLGYNGTSWINLDFTWNDPWIL"
     gene            705..2162
                     /locus_tag="PKM34_10425"
     CDS             705..2162
                     /locus_tag="PKM34_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNQ84048.1"
                     /translation="MSDIYHLKPERLWYFFGEVLKIPRPSKKEEKIIQYLIDFGQNHQ
                     LETIKDEVGNVVIRKPGSHGHENAPWVCLQGHVDMVCEKDADIIHDFDKDPIQAYVED
                     GWLKARGTTLGADNGIAVATMLALLEAKDIAHGPLECLFTIDEETGLTGAFGLDENLL
                     KSRILLNLDSEDDGEFFIGCAGGKDTVIEMNLIKEDIPAGYQTMRMKIFGLQGGHSGD
                     DIHRNRGNANKILNRFLWESAQKFGIRLCSFDGGNLRNAIAREAIGIFLVPAGKTNQV
                     AEFAAGYLEQVRFEFRVSEPDLNLEIEEFETPGFVMAKSTQEYLLNALYACPHGVIAW
                     SQDIPNFVETSTNLASVKMQNGKIRIATSQRSSIESAKEDICNMVASVFKLTGASISQ
                     SDGYPGWAPNPNSKVLELCVSTYEEMFGQKPVVRAIHAGLECGLIGDKYPGMDMISYG
                     PTMRGVHSPTERLEISTVDKFWVFSLELLKKLALV"
     gene            complement(2159..2740)
                     /locus_tag="PKM34_10430"
     CDS             complement(2159..2740)
                     /locus_tag="PKM34_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84049.1"
                     /translation="MVYNRMKTIWLTVFLILCLVPDMISQDTVRMPGRRARSDFWERV
                     DVGGNLGAQFGRVTVLNIEPRISYDITDHLYAGIGVTYLFYRDKRYAPAISLSYYGGS
                     IFGGYYIWRDLYAHVEYAPLYIPDFYDYFIPPIQGMEDQPPWAHDIYLGGGYRQWIGE
                     KIYVNLMILFNINETIYSPYSNPIIRIGFGAGL"
BASE COUNT          785 a          715 c          646 g          667 t
ORIGIN      
        1 agaatccatg gatcattcca tgtaaagtca aggttaatcc agctggtccc attatagccc
       61 aggaaattgg tcccatccca cttaatcgct ccggcattgg aattggccgt gttaccgatc
      121 cttacggcac cgttcacatc cagcttttcc agagggctgt tcgtacctat gcctacattg
      181 cccccttgct ggatgtagat acaatttgca gcagaattct gctcaatgac cataacatcc
      241 agacttaacg catgatcacg gattttaaaa ccacccaggt tggtggcttt aaacttccag
      301 tcggctcctg aaagcgcatc tttcatctgg tatgtcgccc cgccgttccc tccgaggtta
      361 taaacccgga tggtcggttc tgtcatgctt ttggcaacat ccagcagagt tcccggcgcg
      421 ttggtgacaa tacccacatt tcctgcattg gtattgtaaa tatgcgtgcc gttaaacgcc
      481 cactgactat ttacatttac ggtaaaaaac agaaagcaga tgctcccgaa aataaaggcg
      541 atgaactgtt tcatggtgag ttttttttaa ttgataatga agactatgac ccaaacaaat
      601 atacgaaaaa gaaatagata tgtcaaggaa ttcaccattt attttcaata ttcattgcat
      661 caggaaaata ttaactttac agcattaaaa caacatgcaa aacaatgagt gacatctatc
      721 atttgaagcc cgaaaggcta tggtactttt tcggcgaggt attaaagatt ccccgccctt
      781 caaaaaaaga agagaagatc atccagtacc tgatcgattt tggtcagaat caccaactgg
      841 aaacgatcaa agatgaagtt ggcaatgtcg tcatacgcaa gccgggttca cacgggcatg
      901 aaaatgcacc ctgggtatgc cttcaggggc atgtcgatat ggtgtgtgaa aaagatgccg
      961 acatcatcca cgatttcgac aaagatccga tccaggccta tgtggaagat gggtggctga
     1021 aagcgcgggg cactaccctc ggcgctgata atggaatagc tgtggccacc atgctggccc
     1081 tgcttgaagc aaaagacatc gcacacggcc cgctggaatg ccttttcacc atcgacgaag
     1141 aaaccgggct gaccggtgcg ttcggcctgg acgaaaacct cctgaaaagc cgtatcctgc
     1201 tgaacctcga ctcggaggac gatggagaat tcttcatcgg ttgtgccgga ggaaaagata
     1261 ccgtcatcga aatgaatttg ataaaagaag atattcctgc cggataccag accatgagga
     1321 tgaaaatatt cggtttgcag ggcggacatt cgggcgacga tatccatcgc aacaggggca
     1381 atgccaataa aatcctcaac cgtttcctct gggaatcggc tcaaaagttc ggaatcaggc
     1441 tctgttcctt cgacggtgga aatctccgca atgctatagc ccgcgaagcc atcggaatct
     1501 tcctggtgcc ggccggcaaa acaaaccagg tggcagaatt cgctgccggc tacctggaac
     1561 aggtccggtt cgaattcagg gtatcagaac ccgatctgaa cctggagatc gaagaattcg
     1621 agacacccgg gttcgtcatg gcaaaatcga ctcaggaata tcttctgaat gctctctatg
     1681 cttgtccgca tggcgtaatt gcctggtcgc aggacatccc gaactttgtg gaaacctcca
     1741 ccaacctagc ctctgtaaaa atgcagaacg gcaaaatcag gatcgctacc agccagcgga
     1801 gttccatcga atcagccaaa gaagatatct gcaacatggt cgcttcggtg tttaagctga
     1861 ccggagcctc catcagccag tccgacgggt atcccggctg ggcgcctaat ccgaattcca
     1921 aagttctgga actttgtgta agtacctatg aagaaatgtt cgggcagaaa cctgtggtca
     1981 gggctatcca tgccggcctg gaatgcggcc tcatcggcga caaatacccc gggatggata
     2041 tgatcagtta cggaccgaca atgcgtggcg tccactcccc taccgaacga ctggaaataa
     2101 gcactgttga taaattctgg gtattctcgc tggagctgct caaaaagctg gccctcgttt
     2161 agagacctgc gccgaatccg atccggatga taggattgct gtacggcgaa taaatcgtct
     2221 cattgatatt gaaaaggatc atcaggttca cgtagatctt ttcaccgatc cactgcctgt
     2281 accccccgcc caggtatata tcatgtgccc agggcggctg gtcttccatt ccctgtatag
     2341 gaggtataaa ataatcatag aaatccggaa tataaagcgg agcatattcc acatgagcgt
     2401 aaagatcccg ccagatgtaa taacctccga aaatgctgcc cccataataa cttagtgata
     2461 tagccggggc ataacgctta tccctgtaaa ataaataggt caccccgata ccggcataca
     2521 gatggtcggt gatatcatag ctgatccggg gctcgatatt caacactgtc acccttccaa
     2581 actgggcgcc caggttcccc ccgacatcca cacgctccca gaaatcactc ctggctcgcc
     2641 gcccgggcat tcgcaccgta tcctgcgaaa tcatgtcagg aacaagacaa aggatcaaaa
     2701 atacagtcag ccagatcgtt ttcatcctat tgtagaccat taccggatcg tttgatttga
     2761 aactgtaaat ttaccaatat atttttccgg cgatcacgat tgcacaataa agc
//
LOCUS       DAOQXS010000409         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_38876_length_2803_cov_3.420670, whole genome shotgun sequence.
ACCESSION   DAOQXS010000409 DAOQXS010000000
VERSION     DAOQXS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38876_length_2803_cov_3.420670"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1553
                     /gene="guaB"
                     /locus_tag="PKM34_10435"
     CDS             81..1553
                     /gene="guaB"
                     /locus_tag="PKM34_10435"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNQ84050.1"
                     /translation="MTQDNQKFLGEALTYDDVLLVPAYSEVMPREVEINTFFSRNIHL
                     NIPVVSAAMDTVTESMMAIAIAQEGGIGVIHKNMTIEDQANQVRKVKRAENGMILDPV
                     TLPVNALVRDAFMLMKEYGIGGIPVVQGNKELVGIVTHRDLRFERNLERPIAEVMTKD
                     NLITTNEFVSFEKAAEILQKHRIEKLPVVNDHNRLVGLITYKDIIKIQAHPNAYKDSQ
                     GRLRVAAAVGTAPDTLDRAAALVEANVDAIVVDTAHGHTRGVIAIAKRIKKEFPGIDL
                     VVGNIATASAALALAEAGADAVKVGIGPGSICTTRVIAGVGVPQLSAIYDVSRALKGT
                     GIPVIADGGIRYSGDIAKALAAGADTIMAGSLFAGVDESPGETIIYDGRKYKAYRGMG
                     SIEAMQKGSKDRYFQDVEDDIRKLVPEGIVGRVPFKGTLSEVITQLVGGLRAGMGYVG
                     ARNLEMLKQAKFIKITHAGVVESHPHDVSITSEAPNYSRI"
     gene            1596..>2803
                     /locus_tag="PKM34_10440"
     CDS             1596..>2803
                     /locus_tag="PKM34_10440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ84051.1"
                     /translation="MRKIRFIATMSVLLVFMTGGLHAQKTDNRILLNIAGEEVTAGDF
                     MYVYKKNNINREDQGLDSISKYLQLYINFRLKVREAEELGLDTAKAFITELEGYRKQL
                     AQPYFYDSTISEKLIREAYERKQWDLRASHILFTCDKNALPQDTLKAFQSAMAARNRV
                     MAGEDFSKVSVELSQDPNARDSEASQYRPARKGNLGDLGYFTVFDMVYPFESAAYNTP
                     VGTVSMPVRSDHGYHVIKVTDRKPALGQVQVAHIFVSLAAGSTAQDSIQKKIKIDAAY
                     EKIMAGESFEDIVKEFSEDKGSAANGGKLPWFGSNRLVPDFVDAARNLKDSGDISKPF
                     STIYGYHIIKLLGKKPVGTFEEEEFGLKTRIEKDMRNKLSEEAVISRIKQENNFKEYP
                     KAVEAVYATL"
BASE COUNT          768 a          657 c          763 g          615 t
ORIGIN      
        1 aggtcaaagg caacgtggtt gtgatttatt gagttcatta agggattttt actacatttg
       61 caaactaaat ttttctggat atgacgcagg ataaccaaaa attcctcggc gaagccttaa
      121 catatgacga cgtcctcctg gtgccggcct attcggaagt gatgccacgg gaagttgaga
      181 tcaacacctt tttttcgaga aatattcacc tgaatatccc ggttgtatcg gcggcgatgg
      241 acacggtgac cgagtcgatg atggctattg ccattgcgca ggagggtgga atcggtgtta
      301 tccataaaaa tatgaccatt gaagaccagg ccaaccaggt gaggaaggtc aaacgtgccg
      361 aaaacgggat gatcctcgac ccggtgacct tgccggtcaa tgcgctggtt cgcgatgcct
      421 tcatgttgat gaaagaatac ggtatcggcg gcattccggt agtgcaaggc aacaaggaac
      481 tggtcgggat cgtcactcat cgtgatttgc ggttcgagcg caatctcgag cggcccattg
      541 ccgaagtgat gaccaaagac aatctcatca ctaccaatga gttcgtctcc ttcgagaaag
      601 cagcagagat ccttcaaaaa caccggatcg agaagcttcc ggtggtaaat gatcacaacc
      661 gcctggttgg gcttatcacg tataaagata tcattaagat acaggcacat ccgaatgcat
      721 acaaagacag ccagggtcgg ttacgtgtag cggctgcggt agggaccgca cccgatacac
      781 tcgacagggc tgccgctttg gtggaagcca atgtcgacgc gatcgtggtt gatacggccc
      841 atgggcatac ccggggcgtt atcgctatcg ctaaacggat taaaaaggaa tttccgggca
      901 tcgatctcgt cgtcggaaat attgctacgg cttcggccgc gctggcgctg gcagaagcgg
      961 gcgccgatgc ggtcaaggtt gggatcgggc cgggatccat ttgcacaacc cgtgttattg
     1021 cgggggttgg cgtaccgcag ctttcggcca tttacgatgt aagccgggca ctgaaaggga
     1081 ccggtattcc agtcattgcc gatggaggga tccggtattc gggcgacatt gccaaagcat
     1141 tggcggccgg cgccgatacc atcatggccg gttccttgtt tgccggcgtg gatgagtcgc
     1201 cgggtgaaac gattatctat gacggaagaa aatacaaagc ctatcgtgga atgggctcca
     1261 tcgaagccat gcagaaagga tcgaaggacc gctatttcca ggatgttgag gatgacatcc
     1321 ggaaactggt accggaaggg attgtcggcc gggtgccctt taaaggcaca ctgtcggaag
     1381 tcattaccca gctggtaggc ggactcagag ccgggatggg atacgtaggg gcccgaaacc
     1441 tggagatgct caagcaggca aaattcatca aaataacgca cgccggagtc gtcgaaagcc
     1501 atccgcacga cgtatccatt accagcgagg caccgaatta cagcaggatt taatcgcaga
     1561 catatttata aaataacaga aaatcagctt acaccatgag aaaaattagg tttattgcaa
     1621 ccatgtcagt ccttctcgtt tttatgaccg gcgggctcca tgcgcaaaaa acggataaca
     1681 ggatactgtt gaatattgca ggcgaagagg tgacagccgg cgatttcatg tacgtttata
     1741 aaaaaaataa tatcaaccgg gaagaccagg gattggacag tatctccaaa tacctgcagc
     1801 tttatatcaa tttcaggttg aaagtcaggg aagcggaaga gctggggctc gatacggcca
     1861 aagcttttat taccgaactg gaaggatacc ggaaacaatt ggcccagccc tatttttatg
     1921 attccaccat cagcgagaaa ctgatcaggg aggcttatga gcgcaaacaa tgggatctcc
     1981 gcgccagcca tatattgttt acgtgtgata aaaatgcact gcctcaagat acattgaaag
     2041 ccttccagag tgcgatggct gcccgcaaca gggtgatggc cggcgaggat ttttcaaagg
     2101 tgtcggtaga actttcacag gatccgaacg caagggattc tgaagccagc cagtaccgcc
     2161 cggcccgcaa aggcaacctg ggcgacctgg gctattttac ggttttcgat atggtttacc
     2221 cgttcgaatc ggcggcctac aacacccccg tcggaacggt ctctatgccg gtccgcagcg
     2281 atcatggtta ccatgtgatc aaggtaaccg accgaaaacc agcactgggc caggtacagg
     2341 tggcccatat cttcgtcagc ctggcagccg gttcgacagc ccaggattcc atccagaaaa
     2401 agattaagat cgatgctgct tacgaaaaga tcatggccgg tgaaagtttt gaggatatcg
     2461 tcaaggaatt ttcggaagat aaaggatcgg cagcaaacgg gggaaaactc ccctggttcg
     2521 gatcaaaccg gctggtcccc gattttgtcg atgcggccag aaacctgaaa gattcaggcg
     2581 atatcagcaa acccttcagt accatttacg gctatcatat cattaaacta ttgggtaaga
     2641 agccggtagg tacttttgag gaagaggagt tcggccttaa aacgcgtata gaaaaagaca
     2701 tgcgcaataa gctcagtgaa gaggccgtaa tcagtcgtat caagcaggag aataacttca
     2761 aagaatatcc caaagcggtc gaagccgttt atgcaaccct cga
//
LOCUS       DAOQXS010000410         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_39296_length_2782_cov_3.137880, whole genome shotgun sequence.
ACCESSION   DAOQXS010000410 DAOQXS010000000
VERSION     DAOQXS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39296_length_2782_cov_3.137880"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKM34_10445"
     CDS             complement(<1..314)
                     /locus_tag="PKM34_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNQ84052.1"
                     /translation="MSTVYLYPIWTRLWHLFNALLCLALIFTGLSMQYSNPEYPLIRF
                     DIAVSIHNISGILLSINYLMILIGNLATGNGKYYWIRIKGFYNELLAQGKYYMFGMFR
                     GE"
     gene            complement(320..1282)
                     /locus_tag="PKM34_10450"
     CDS             complement(320..1282)
                     /locus_tag="PKM34_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNQ84053.1"
                     /translation="MFKNYKYLLFLLICITGHRAPAQEESEIAPITIEKNNECLVCHG
                     YKYFSYFNEYYGRDVKERMNPLYIVDSTEFYLSNHRTFMCTDCHSTEYGDEFPHPNHL
                     RFEPSFSCMDCHEGNDEFARFNFETIRDEFVKSVHSEKHSEDFTCWMCHNPHSYHISA
                     RNSQNLTETIRYDNQICLDCHSDEGRIGLLSDREIFNMLDKHEWLPNHLAHFKSVRCI
                     ECHAEVNDDLMVAHNILPKEKAVKKCVECHSQNSILMASLYKHQVQEKRSSRGYFNAA
                     IMTETYVIGANRNIFLNIASISIFGLAFIGVIIHAILRKIYAKK"
     gene            complement(1491..2405)
                     /locus_tag="PKM34_10455"
     CDS             complement(1491..2405)
                     /locus_tag="PKM34_10455"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNQ84054.1"
                     /translation="MKKYILFILVSFSVLSLMAQDTETPKKENKNKSLLGYFILNDSK
                     PLTTYEGGMVIDNQTSYIPSAKTLELLMQHRFGSMNNGLSDFLGIYGAANARMALNYS
                     VTDWLQLGLGTTKNYKLQDLILKVNLLRQSRDKKSPVDVTFYGNYSVDARDESFFGED
                     YQFSDRMAFFNELLVSRQVTKWFTVSAGGSFTHFNQVDSLFEHDKIALHFLGRFKFSS
                     QSAIIVNCDLPLKIDGIKEWLEFTDPPKYNLGIGYEVVTATHVFQIFAGSATSLVPQY
                     NVMLNQNDFFKGTDNIFIGFNISRVWNF"
     gene            complement(2437..>2782)
                     /locus_tag="PKM34_10460"
     CDS             complement(2437..>2782)
                     /locus_tag="PKM34_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84055.1"
                     /translation="GIMVWQSCQYDWYDPVDYIPPPPGDTTYFSLEIIPIFNNSCNSG
                     SCHGAGGIPPDLSPANAYADLFARNQIDTVTPENSKLYIKCAPGGSMNKYCQPGDPEL
                     ILQWIKEGALNN"
BASE COUNT          729 a          584 c          682 g          787 t
ORIGIN      
        1 ttttcacctc gaaacatccc gaacatgtaa tattttccct gggcgagcaa ttcattataa
       61 aatcctttga tcctgatcca gtaatatttt ccgttgcctg ttgccaggtt tccgatgagg
      121 atcatcaggt aattaataga taagagaata ccactgatat tatggatgct gaccgcaata
      181 tcaaagcgga tcaacgggta ctcgggattg gaatattgca tgctcaggcc tgtaaagatc
      241 agggcgaggc agaggagtgc attgaaaagg tgccagaggc gtgtccagat agggtagagg
      301 tatacagtcg acatttcagt tattttttag cgtagatttt tctcaggatt gcatgaatga
      361 tcaccccgat gaaagccagc ccgaagatgc tgatactggc aatattcagg aagatgttcc
      421 ggttggcacc aatgacgtaa gtttctgtca taattgcggc gttgaaatac ccccgtgaag
      481 atctcttttc ctgcacctgg tgtttgtaaa gcgatgccat caggatggag ttctgggaat
      541 ggcactcgac acatttttta accgcttttt cttttgggag gatattatgg gcaaccatga
      601 ggtcatcatt cacttcggca tggcattcaa tgcaccggac acttttaaaa tgggccaggt
      661 gattgggaag ccattcgtgt ttatcgagca tattaaaaat ttcccggtcg gagagcaggc
      721 cgatccggcc ttcatcggag tggcaatcca ggcagatctg gttgtcgtac cggatagtct
      781 cggtgaggtt ctggctgttc cgggcactga tatgatatga gtgcggattg tggcacatcc
      841 agcaggtaaa atcttcgctg tgtttctccg aatgaacact tttcacaaat tcatcccgga
      901 tagtctcaaa attaaaccgg gcgaattcat cgttgccttc gtggcaatcc atacacgaaa
      961 aactgggctc aaagcgcagg tggttcggat gcggaaactc atccccgtat tctgtggaat
     1021 ggcaatcggt gcacatgaag gtacggtggt tcgacaggta gaattccgtg gaatcgacga
     1081 tatagagcgg attcattctt tccttcacat ccctcccgta atattcattg aaataggaaa
     1141 agtatttata tccgtggcaa acaaggcatt cgttattttt ttcaatggtg ataggtgcaa
     1201 tttcagattc ttcctgtgcc ggagcccgat ggccggtaat gcagatgagc aggaagagca
     1261 ggtatttata gtttttaaac atttataaat cgttaaagtg gtttatacag tcaatttaga
     1321 aggtgtctca tacaccttct atttaaccac aaaggcgcac aaagtactca cgacggaaca
     1381 caaaatattt aattataaaa gtagtgatct ttgtgttcct ttgtgtaatc ctttgtgttc
     1441 ctttgtggtt ttatcttttt ttatacaaac tctaaaaaaa tcaaacctca tcaaaaattc
     1501 cagacacggg aaatattaaa cccgataaaa atattgtcgg tccctttgaa aaaatcattc
     1561 tggttgagca tgacattata ctgcggaacg agtgaagtcg ctgatccggc gaagatctgg
     1621 aaaacgtggg ttgcggtaac aacctcgtag ccgattccca ggttatattt cggggggtcg
     1681 gtaaattcaa gccattcctt gatgccgtcg attttcagcg ggaggtcaca attcactata
     1741 atagccgatt gggagctgaa tttgaaccgt ccgagaaaat ggagggcgat tttgtcatgt
     1801 tcaaagagcg aatcgacctg gttgaaatgg gtaaagcttc ccccggccga aacggtgaac
     1861 cattttgtta cctgcctgga aaccagcaat tcattgaaga aagccattct gtcggagaac
     1921 tggtaatctt cgccgaagaa actttcgtcg cgggcatcaa cggagtagtt tccatagaag
     1981 gtcacgtcga cgggcgattt cttatccctg ctttgccgca ggagattgac tttcaggatc
     2041 aggtcctgga gtttgtaatt tttggtggtg cccaggccca gttgcagcca gtcggtaacg
     2101 gaatagttaa gcgccatgcg tgcattggca gcgccgtata tcccgagaaa atcggagagc
     2161 ccgttattca tcgaaccaaa acggtgttgc atcagcaact ccagtgtttt ggccgaaggt
     2221 atgtaactgg tctggttatc gatcaccatt ccgccttcat aggtagtcag gggcttgctg
     2281 tcatttaaaa taaagtagcc cagcaggctc ttgttcttgt tttccttctt gggtgtttca
     2341 gtatcttgag ccattaaaga aaggacacta aaactgacca ggatgaaaag aatatacttt
     2401 ttcatgttca ttcggttttg aatttttgaa ttccggttaa ttattcaaag cgccttcttt
     2461 tatccactga aggataagct caggatctcc gggctggcaa tatttgttca tggagccacc
     2521 cggagcgcat ttgatgtaca atttgctgtt ttccggtgta acggtgtcga tctggttcct
     2581 tgcgaacagg tcggcgtagg cattggccgg tgaaaggtcc ggcgggatgc ctccggcgcc
     2641 gtggcaactg cccgaattac agctattgtt aaagatcggg ataatttcaa gcgagaaata
     2701 agtggtgtcg ccgggtggtg gtgggatgta atccaccgga tcataccagt cgtactggca
     2761 cgactgccaa accatgatcc cc
//
LOCUS       DAOQXS010000411         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_39634_length_2766_cov_3.231280, whole genome shotgun sequence.
ACCESSION   DAOQXS010000411 DAOQXS010000000
VERSION     DAOQXS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39634_length_2766_cov_3.231280"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1271)
                     /gene="dnaJ"
                     /locus_tag="PKM34_10465"
     CDS             complement(117..1271)
                     /gene="dnaJ"
                     /locus_tag="PKM34_10465"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNQ84056.1"
                     /translation="MAKRDYYEVLDIPRSASADEIKKAYRKMALKYHPDKNPDDPSAE
                     AKFKEAAEAYEVLSDSDKKQRYDRYGHDGVKGMNGGGFNMSMDDIFSHFGDIFGGGFG
                     SAFGEQFGFGTRGRTTRRVNRGTNLRVKVKLTLEEIASGVEKKIKVSKYIPCDHCNGT
                     GAEKGASMNTCPTCHGQGRVTRVTSTFLGQMQTSSTCPTCDGEGTIIGTKCNKCFGNG
                     IVKGEEVIAVRIPAGVADGMQLSMSGKGNAAARGGIPGDLLILIEEHEHEHFTRDNNN
                     LVYEHFISLPDAALGGSVEIPTLDGKARLKIDPGTQSGKILRLKNKGLPDINGYGRGD
                     LLVNLTVWTPRELTREEHQIMEKLRESENFKPHPSKKDRGFFDRMKDLFQ"
     gene            complement(1273..1872)
                     /locus_tag="PKM34_10470"
     CDS             complement(1273..1872)
                     /locus_tag="PKM34_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNQ84057.1"
                     /translation="MTSKEDKNKKTETTEDQSVKTGSDSHSGPASEESPAKKDKKKKH
                     DDSEELNRQWQAKYDEVNDKYLRMYSEFDNYRKRTLKEKVEFSKTASEEIIVALLPVI
                     DDLERALATGFKTEDGKEVVMMEGLTLIYQKFKGLLAQRGLEPVPSVGEAFDVDFHDA
                     LTNIPAPSDELKGKVIDEIEKGYKLNGKVIRYSKVVVGN"
     gene            complement(2055..>2766)
                     /locus_tag="PKM34_10475"
     CDS             complement(2055..>2766)
                     /locus_tag="PKM34_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ84058.1"
                     /translation="LQLCRMLNSMGARISGVGSNLLTITGVDYLAGCIHTMMPDMIEV
                     GSFIGLAAMTQSELTISGVRLEDLGLIPTVFNRLGITVEQAGENIHIPQHTRYEVETF
                     MDGSIMTIADAPWPGFTPDLISIVLVVATQAKGSVLIHQKMFESRLFFVDKLIDMGAQ
                     IILCDPHRATIIGLNREHMLRGIDMVSPDIRAGVALLIAALSAEGRSVIHNIEQIDRG
                     YQNIDSRLAALGADIRRI"
BASE COUNT          581 a          725 c          653 g          807 t
ORIGIN      
        1 tgctccgttt ggcccgagaa ggccgtagat gcttcctgcg gggacatcga gggtgacttt
       61 ttccagcgcg gtgtgtccgg aaaaacgctt ggtgacttct tctacctgta ttatgttcac
      121 tggaataaat ctttcattct gtcaaaaaat cctctgtctt ttttcgaagg gtgcggctta
      181 aagttttccg attccctgag cttttccatt atttggtgtt cttcgcgggt cagctccctg
      241 ggagtccaga cggtcaggtt gaccagcagg tcgcctcttc cgtacccgtt aatatcgggc
      301 agccctttgt ttttcaaacg cagtattttt ccgctttgcg ttcccggatc tattttcagc
      361 cttgcttttc cgtcgagagt cgggatttca actgaaccgc cgagggctgc atcgggaagg
      421 ctgataaaat gttcatagac caggttgtta ttgtcgcgtg taaagtgctc atgttcatgt
      481 tcttctataa ggatgagcag atcccctggg ataccgccac gggctgccgc attgcctttg
      541 ccgctcatag agagttgcat tccgtcggct acgcctgccg gaatcctaac agcgatcacc
      601 tcttctccct tgacaattcc gttgccaaag catttgttgc acttggtgcc gatgatcgtc
      661 ccttcgccgt cgcaggtcgg gcaggtggat gatgtttgca tctgtcccag gaaggttgag
      721 gttacccggg tcacccttcc ctggccatgg cacgtcggac acgtattcat cgaggctcct
      781 ttttcggcgc ccgttccgtt acaatggtca caggggatgt atttgctgac ttttatcttt
      841 ttctctactc cagacgcaat ctcttccagg gtcagtttga ccttcaccct caggttggtt
      901 cccctgttca cccttcgggt ggtcctgccc cgcgttccga aaccaaactg ttccccaaag
      961 gcgcttccga aacctccccc gaaaatatcc ccgaaatggg agaatatatc gtccatgctc
     1021 atattgaagc cgcccccgtt catccccttc accccgtcat gcccgtaacg gtcgtaacgt
     1081 tgttttttat cagaatcact aagcacctca tatgcttctg ccgcttcctt gaatttagcc
     1141 tcggccgatg ggtcatccgg atttttatcc ggatggtatt tgagggccat tttccggtag
     1201 gctttcttga tctcgtcggc agaagccgaa cggggaatat caagaacttc gtaataatcg
     1261 cgttttgcca tcttaattcc ccactacaac ttttgaatac cggatcactt taccattcag
     1321 tttataccct ttctctattt cgtcgatcac tttacctttc agctcatcgg aaggggcagg
     1381 gatattggtc agcgcatcat gaaaatcaac atcgaaagct tccccgacgg aagggacggg
     1441 ttcaagcccg cgctgagcaa gtaatccttt aaatttttga tagatgagcg taagcccttc
     1501 catcatcacc acctctttgc catcttcggt tttaaagccg gtggccaggg ccctttccag
     1561 atcatcgata acaggtaaga gggcaacaat tatctcttcg gatgcggttt tgctgaattc
     1621 taccttttct ttcagtgtcc gtttacggta attatcaaat tctgagtaca tccggagata
     1681 tttgtcatta acctcgtcgt atttagcctg ccactgccgg ttcagttctt cggaatcatc
     1741 atgtttcttt ttcttatctt tttttgcagg gctttcttca cttgccggtc ctgaatggga
     1801 atcactgccg gtttttaccg attgatcttc ggtggtttcc gtttttttat ttttatcttc
     1861 tttgctcgtc atggtattta tctggctata ataataaata caccctttaa agattgatat
     1921 tcaataaatt ccttctttac aagggtgcaa aaatagtgaa acttcgcgct ttgcgatcaa
     1981 attgtttgcc aaccgcgata aaaggacaat ttggcagggc taagtccccg acaggaaaag
     2041 acggcatgca gatttcagat gcgcctgata tcggcaccca gggcggccag tcgtgaatcg
     2101 atgttctggt acccccggtc gatctgctcg atattatgga tcacgctacg gccttcagcc
     2161 gagagcgcag ctatcagcaa ggcgacgcca gcccgtatgt cgggcgatac catatcgatc
     2221 cccctgagca tgtgttcgcg gttcaggccg atgattgtag cgcggtgcgg gtcgcagaga
     2281 atgatctggg cgcccatgtc gatcagttta tcgacaaaga aaaggcggct ctcgaacatt
     2341 ttctggtgga tgagtacact tcctttagcc tgtgtcgcta ctaccagcac aatgctgata
     2401 aggtcggggg tgaatccggg ccacggtgca tcggcaatgg tcatgatgga tccatccata
     2461 aaggtttcca cttcgtagcg ggtatgttgc ggaatatgaa tattctcacc tgcctgctcc
     2521 accgttatgc ccaggcggtt gaagacggtc gggatcaatc ccaggtcttc cagcctgacg
     2581 ccggaaatgg tcagttccga ctgtgtcata gcagcaagac cgatgaaact gcccacttcg
     2641 atcatatcgg gcatcatggt atgtatgcat cccgccagat aatcgactcc tgtaattgtg
     2701 agcaggttgg agcctactcc gctgatccgg gcacccatgc tgttcagcat acggcataac
     2761 tggagg
//
LOCUS       DAOQXS010000412         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_40492_length_2728_cov_3.512159, whole genome shotgun sequence.
ACCESSION   DAOQXS010000412 DAOQXS010000000
VERSION     DAOQXS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40492_length_2728_cov_3.512159"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /locus_tag="PKM34_10480"
     CDS             <1..2059
                     /locus_tag="PKM34_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84059.1"
                     /translation="IYPNITSPATEHVNQFNWQYDYIPGLPYGLPAETNCISTLSSYA
                     PKSIIPWVGMDAPGTFSCPVSVVDFLNIKSFSLIFRYDPLVMTYISNTPNPVFNATNG
                     YLTVTDASAAGGLRRITMTFNGLNTISLPDNSILCGFQFNYIGGEAAIGWRTNSSGCT
                     YYGPGGILKCDQPYSNYFIDGGIVSMLAPVTKVDSAALSPGEFGTFTVRVWDFQDIRA
                     GILTLGYNPAALIYQETIPNPALGEEFEANLVNPGLLQFSWSEAGITLADASIIVHLT
                     FQYLGGNSTLYWINDGTSCQYFHSNIPSPMTDIPTSSYYFNGNVENAVYIWSGDNSSE
                     WGSDGNWENGEAPNQNMDVIVDPTVNPDNWPVFDGDFTLGEHCRNLIINGNAQLIING
                     NLNVLPGRALSLTGNGILKVDGNWTNSGIFYPGDGTIEFTGTTNAAIAAGDPPGNYVS
                     NYSLTPFSSTFIPLSGGLPGPAGNDSHSDVPIGFPFTYLGVSYSMARINTNGWLSLNL
                     SGEDTGSGNNLALFETAGASTALAPWWDDLSADAGASVTYLTEGIAPFRVFTTEWYNV
                     LSYSTGSASRLNFQVKLYETLNIIEFYYGTAISGYNSPDEGASIGIKDAMGGQGHYIE
                     ATQNSTDIILAFLNSSTDWPAQNFRFIPPLTTDMEIFHKIIVNKPGANLSIQKDIKIT
                     GLE"
     gene            2121..>2728
                     /locus_tag="PKM34_10485"
     CDS             2121..>2728
                     /locus_tag="PKM34_10485"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84060.1"
                     /translation="MKKISLIMLITGFVFSGFYAQSQRYDKGAQYCSHRKSMLKNTPA
                     NVRSENSPRHRFDVLNYKLNMDLWDNFQNPFPQAFDATETVTFRVDTALNQIKLDAVN
                     TSLTIHSVGLAGTGYTHLDDTLTVQLDRIYNPGEIAEVSIDYSHKNVEDEAFYVSGGF
                     VFTDCETEGARKWFPCYDRPADKATVDITAKVPGNVLLGSNGR"
BASE COUNT          692 a          721 c          617 g          698 t
ORIGIN      
        1 gatctatccc aatatcactt ctccggcaac ggaacacgtc aaccagttca actggcaata
       61 cgattacatt ccgggattac cctacggatt acccgctgaa accaattgca tttccacact
      121 cagttcatac gccccgaagt cgatcatacc ctgggtgggc atggatgccc cggggacatt
      181 ctcctgccct gtcagcgtag tagattttct gaacatcaaa tctttctcac tgatcttccg
      241 gtatgacccc ctggtaatga cctatatcag caatacgccc aatccggtct tcaatgcaac
      301 aaacggatac ctgaccgtta ccgatgcctc ggctgcaggt ggcctgagaa gaattacgat
      361 gacatttaat ggtttgaaca ccatttcgct tcctgataac tcgatccttt gcggatttca
      421 gttcaattat atcggtggtg aagcggctat tggctggcgg accaactcca gcggttgcac
      481 ctattatggc ccgggtggga ttttgaaatg cgatcaaccc tattccaatt atttcatcga
      541 cgggggtata gtatcgatgc tggccccggt gaccaaagtt gattcggcgg cactgtcgcc
      601 cggcgaattc ggcactttta ccgtcagagt atgggatttt caggatatac gtgctggtat
      661 tctcactctg gggtacaacc cggcagccct gatataccag gaaaccatac ccaatcccgc
      721 cctcggggag gaatttgaag ccaacctggt caaccccggg ctgctccagt tcagctggtc
      781 tgaagcaggc attactcttg ccgatgccag cattattgta cacctcacct ttcaatacct
      841 tggcggaaat tcgacactgt actggattaa tgacggcacc tcctgccagt atttccatag
      901 taatataccc tcacctatga ccgatattcc gacatccagt tattacttta acggtaacgt
      961 cgaaaatgct gtatatatct ggtcgggtga taattcaagc gaatggggtt cggacgggaa
     1021 ctgggaaaac ggtgaagccc cgaaccagaa catggatgta atcgtcgatc cgactgtcaa
     1081 tccggataac tggcccgtat tcgatggcga ttttacactc ggagaacatt gccggaacct
     1141 gatcattaac ggcaatgcac agctgatcat caacgggaac ctgaatgtac tgccgggtcg
     1201 tgccctaagt ctgacgggaa acggcatctt aaaggttgac ggcaactgga ccaattccgg
     1261 gattttttac ccgggcgacg ggaccattga atttaccgga acaaccaatg ccgcaattgc
     1321 tgcgggcgat ccaccgggaa actatgtttc caattactca cttaccccgt tctcttcaac
     1381 ctttataccc ttgagcggag gactgccggg gcctgccgga aacgattccc attcggatgt
     1441 tcccatcggc ttcccgttta cttacctggg agtcagttac tctatggcca ggataaatac
     1501 caatggctgg ctatcgctta acctcagcgg agaagatacc ggctcgggaa ataatctggc
     1561 cctttttgaa accgcaggcg cctcaacggc ccttgctccc tggtgggatg acttgtcggc
     1621 cgatgccgga gcatccgtca cttacctcac tgaaggtatt gcccctttca gggtgtttac
     1681 aaccgaatgg tacaatgttc tgtcgtattc aacaggttcg gcttcccggc tcaatttcca
     1741 ggtaaagctg tatgaaacgc tcaacatcat cgaattttat tacggcacgg caatatcagg
     1801 ttacaacagc cccgatgaag gagcctctat cggaatcaaa gacgctatgg gcggacaagg
     1861 ccattatatc gaagccacac agaattccac cgatattatc ctggcattcc tgaacagcag
     1921 caccgactgg cccgctcaga atttcaggtt tattcctccc ctgacaaccg atatggaaat
     1981 cttccataaa attatcgtca acaaaccggg cgctaatctc tccatccaga aggatataaa
     2041 aataacggga ctggaataaa ttcttaccag gctccgttca aatctttttt aggattcttt
     2101 cattaccttt gagttttttt atgaaaaaga ttagtttgat catgctgatc actggatttg
     2161 ttttcagtgg tttttatgcc caatcccaac gttacgataa aggggcacag tattgttccc
     2221 acaggaagtc gatgctgaaa aacactcctg ctaatgtcag aagtgaaaat tcaccccgtc
     2281 accgcttcga tgttctgaat tacaaattaa atatggatct ttgggataac ttccagaatc
     2341 ctttcccgca ggcctttgat gcaacggaaa cggttacctt cagagtcgac acagcgctga
     2401 accaaatcaa gctcgacgct gtcaacacct ccctgaccat tcattcggtt gggctggcag
     2461 gtaccggtta tactcatctc gatgatacgc ttactgttca actggaccga atctacaatc
     2521 ccggggaaat cgccgaggtt tccattgatt acagccataa aaacgtggaa gatgaagcgt
     2581 tttatgtcag cggcggtttt gtttttaccg actgcgaaac ggaaggcgcc cggaagtggt
     2641 ttccctgcta cgaccggcca gccgacaaag ccactgtgga tatcactgca aaagttcccg
     2701 gcaatgtcct cctgggttca aacggccg
//
LOCUS       DAOQXS010000413         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41090_length_2704_cov_3.591544, whole genome shotgun sequence.
ACCESSION   DAOQXS010000413 DAOQXS010000000
VERSION     DAOQXS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41090_length_2704_cov_3.591544"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKM34_10490"
     CDS             complement(<1..181)
                     /locus_tag="PKM34_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ84061.1"
                     /translation="MVKIGVLGAGHLGKIHIKCIRDIKNFELVGFHDADVKTASNVKS
                     EFGLYHFENIEELIDS"
     gene            297..947
                     /locus_tag="PKM34_10495"
     CDS             297..947
                     /locus_tag="PKM34_10495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNQ84062.1"
                     /translation="MIQDTFRQKGLRKKLVDILRDKGISDERVLNAINRVPRHLFLDS
                     SFLEFAYQDKAFPIGSGQTISQPYTVAFQTELLQVREGDKILEIGTGSGYQSCVLIEM
                     GAKVFTIERQKNLFDRTKAFLPGIGYFPKMFYGDGYKGLPSFAPFDKILITAAAPGIP
                     DELIRQLKNDGIIVAPVGSGESQTMMAMEKINDQQKKISEHGKFRFVPMLNKKAKD"
     gene            1063..1965
                     /locus_tag="PKM34_10500"
     CDS             1063..1965
                     /locus_tag="PKM34_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84063.1"
                     /translation="MKKYLYFIGAFVIASSLFLSSCTEDEDPVDLPPSISFVVEDGFI
                     SGNTTLTINTPFNVKLLAVANATSASKIASIEINRIYNNQTIWDTTLTYNDATVNLTV
                     GFVALANAGVENIEFIATDKDGQTDKINLQITTEEPAGGPINLWETRTLGSWNNLTIG
                     SSFGSINGNVYMLNEAFANQALIDFIYWWGASTDATIGAPNDANAALVFNTGAYALNN
                     WTTRNATMFKTTTVNPAAFDAIDDATECIDIATGADQTRIGTLAADQVIAFVTVTGKH
                     GLIKVKSIETGSAGSITIDVKVEQ"
     gene            2109..>2704
                     /locus_tag="PKM34_10505"
     CDS             2109..>2704
                     /locus_tag="PKM34_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84064.1"
                     /translation="MNEILGKLPHHLMQLVIDQPYNSYTAQDHAVWRYVMRQNVRYLS
                     KVAHGSYLDGLKRTGISIDSIPHMYGMNRILKEIGWAAVAVDGFIPPAAFMEFQAYNV
                     LVIAADIRPIDQIEYTPAPDIIHEAAGHAPIIADPEYAEYLKLFGRIGSRAFASARDH
                     EIYEAVRHLSILKADPYTNPESILQAGKELAEAEAIHET"
BASE COUNT          762 a          607 c          606 g          729 t
ORIGIN      
        1 cagaatcaat aagttcttca atattctcaa aatgataaag accgaattcc gattttacat
       61 tggatgccgt tttaacatct gcgtcgtgaa atccgactag ttcaaaattt ttaatatccc
      121 ggatgcactt gatatggatt ttacccaggt gcccagctcc caatacaccg atctttacca
      181 tttattattt tttgtgcaaa tgtagctttt tgaccataac catcgaagga aaggtttgtc
      241 atcttttcaa cggaattatt attttcgcgg caacaagaag gttaattgtt ttcatcatga
      301 tccaggacac atttcgtcaa aaagggctca ggaaaaaatt ggttgatatt ttgcgggata
      361 aaggcatctc cgatgaaagg gtattgaatg ccatcaaccg ggttcccagg cacttgttcc
      421 tggattcgtc attcctggaa ttcgcttacc aggataaggc atttccgata ggatcgggtc
      481 agacgatctc gcaaccttat accgtcgcat ttcaaacgga gctattacag gtaagggaag
      541 gcgataaaat attggaaatt gggaccggct cgggttacca gtcgtgtgtt ttaatcgaaa
      601 tgggagctaa agtttttacg attgagcgtc aaaagaattt attcgacagg acgaaagcgt
      661 tccttccagg tatcggttat tttccaaaaa tgttttatgg tgatggatac aaagggctcc
      721 cctctttcgc cccttttgat aaaatactca ttacggctgc tgcgccgggc attccggacg
      781 aactaatccg tcaactcaaa aatgatggca taattgttgc tcctgtcggg tccggtgaat
      841 ctcaaaccat gatggccatg gagaagatca atgatcaaca aaaaaaaatt tcggaacacg
      901 gcaagttcag atttgtgccc atgttaaata aaaaggctaa ggattgattg attggttaaa
      961 tttcaacatg tacttagaat gattttaagt aaatttgctg cggaataaca attaatctgt
     1021 aatcacgtta aactctatta ttaactaata attaaaaaga aaatgaaaaa gtatttgtat
     1081 ttcatcggag cgtttgtcat cgcctcatca ttgtttttat cctcatgtac ggaagatgaa
     1141 gatccggtag atcttccacc aagcatcagc ttcgttgttg aagacgggtt tatttcaggc
     1201 aataccacgt taacgatcaa cacaccgttt aatgtaaagt tgctggccgt tgccaatgcg
     1261 acctctgcat cgaaaatcgc aagcattgaa attaaccgta tttacaataa ccagacgatc
     1321 tgggatacga cgctcactta taatgacgca acggtcaacc tgactgtcgg ttttgtagcc
     1381 ctggcgaatg ccggcgttga gaacatcgaa tttatcgcta ccgataagga cggacagact
     1441 gacaagatca accttcagat cacgaccgaa gaaccggccg gtggtcccat caacctgtgg
     1501 gagacccgta cgctcggatc atggaacaat ctgacgatcg gcagttcatt cggatccatc
     1561 aacggaaacg tttatatgct gaacgaagca ttcgccaacc aggcactgat cgatttcatt
     1621 tactggtggg gcgcttcaac agatgcaacc attggtgcgc ctaatgatgc caatgctgcc
     1681 ctggtattca acacgggagc ttatgccctg aataactgga ccacacgcaa tgcgaccatg
     1741 tttaaaacca ctacggtcaa tccggctgcc tttgatgcca tcgacgatgc gactgagtgc
     1801 atcgatatcg ctaccggcgc agaccagacc cgtatcggca ctcttgctgc cgaccaggtc
     1861 attgctttcg tcacggtgac gggaaagcat ggtttgatca aggtgaagag cattgaaacc
     1921 ggttctgccg gatccattac cattgatgta aaggttgagc aataggatat tataaatctc
     1981 cggaagaggc tgcccggttc atcggacagc ctcttttttt ttaacatttt tttggttaaa
     2041 ttttcgttaa tcaatttcgg agaagcctga aagtacatga aaaatggtaa ttttggaaga
     2101 tgatggaaat gaatgaaata cttggcaaac tcccgcatca cctcatgcaa ctggtcatcg
     2161 atcagcccta taattcctat acggcgcaag accatgccgt ttggcgttat gtgatgaggc
     2221 agaacgtcag gtatctgagt aaagtggcac acggctccta cctcgacgga ttaaaaagga
     2281 ccggcatctc catcgacagc attccgcata tgtacggtat gaaccggatc ctgaaagaga
     2341 tcggatgggc ggcagttgcc gtcgacggtt tcatcccgcc tgccgcattc atggaattcc
     2401 aggcctacaa tgtgcttgtt attgcagctg atatccggcc gatcgaccag attgaataca
     2461 ccccggcccc tgatattatt catgaagcag ccggacacgc acccatcatc gctgatccag
     2521 aatatgcgga atatctgaag ttgtttggcc ggatcgggtc aagagccttt gcttctgcca
     2581 gggatcacga gatttacgaa gccgtcaggc atctttctat tcttaaagcc gatccttata
     2641 ctaatcctga atccattctg caggccggga aagagctggc tgaggccgaa gcgatccatg
     2701 agac
//
LOCUS       DAOQXS010000414         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41259_length_2698_cov_3.708664, whole genome shotgun sequence.
ACCESSION   DAOQXS010000414 DAOQXS010000000
VERSION     DAOQXS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41259_length_2698_cov_3.708664"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PKM34_10510"
     CDS             complement(<1..1619)
                     /locus_tag="PKM34_10510"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNQ84065.1"
                     /translation="MLKRLVFLFILIPLTGILMAQYNHAEQDDAPYCYHVKSAAMAAN
                     EYYAPVVTNPLLEKYDVTFYFLDLNVENDTVAVSGSVTTKAIVTGTLMDTIVYELIDE
                     MTVDTVYVNGIQHSFVHNNDLILVPIEPIPNGVYFTTKICYHGTPPTGGFFSGVTTAY
                     STPFEKNVTWTLSEPYAAKDWWPTKQDLTDDADSVWVFLTTSAENKAGSQGLLTAVTP
                     VPGGKLRYEWKSYYPIDYYLISFAVADYQEYNIYAYPAGTEDSVLIQNYIYDHVNCLP
                     LYQVGIDRTAGFIELFSDLFGLYPFIDEKYGHCLTQLGGGMEHQTMTTIGGFSFNLVA
                     HELGHMWFGDKVTCATWSDIWINEGFATYSDYLAVSNLLSPAEDSAWLRSKHNQVKSA
                     PGGSVYVPEEELDNIYRIFNGRLSYSKGALLLHMIRFELQDDEMFFNVLRTYMEEYGD
                     SVATGADFREWLEYISGEDFTDFFDQWYYGEGYPIFDIVWNQNNGNLNVSSTQTTSTT
                     VTQLFKMKVPYHVVYSAWCRYHRNKPHDKGLS"
     gene            1739..>2698
                     /locus_tag="PKM34_10515"
     CDS             1739..>2698
                     /locus_tag="PKM34_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84066.1"
                     /translation="MTEDNYSILIRKLDEFIRKFYRNRLIRGAIFYVAIVTIAYLLIT
                     LTEFFGRFSPGIRTLFFYSFLVLSAGFLWYYILVPVIRLLGFGKIISHEEAAEIIGKH
                     FPEISDKLLNTLQLKKLQEHHTDEFPIELIRAGIDQKIEKLRPVPFALAIDFRKNGRY
                     LRYALPPVIIIILLLIMSPRVITEPSERIVKYQTVYLPPLPFTVQILNEKLESFQQED
                     FLLELKAEGEELPAELFVETGGVRYRMERSSPVLFRYNFRNLQETKLFIISNDLFKSE
                     EYQLTVYPKPIVLSFEISLDYPVYTGKSDEKSENTGDLIVPEGT"
BASE COUNT          714 a          641 c          607 g          736 t
ORIGIN      
        1 gaaagtccct tatcatgtgg tttattccga tggtaccgac accacgctga ataaaccaca
       61 tgataaggga ctttcatttt gaaaagctgc gttacagttg tcgatgtggt ttgggtcgat
      121 gaaacgttca ggttcccgtt attctggttc cagacaatat cgaaaatcgg gtatccttca
      181 ccgtaatacc actggtcgaa aaagtcggta aaatcttcac ccgatatata ctccagccac
      241 tccctgaaat cagctcccgt tgccactgag tcaccgtatt cttccatgta ggtgcggagc
      301 acattgaaga acatttcgtc atcctgcaat tcgaaccgga tcatgtgaag cagcaatgcc
      361 cctttgctgt aagataacct gccgttgaaa atccggtaga tattgtcgag ttcctcttcc
      421 ggaacatata cgctgccgcc gggagcgctc ttcacctggt tgtgcttgct tctcagccag
      481 gccgaatctt cggcgggcga cagtaagttg gagacggcaa gataatcgct gtatgtggcg
      541 aatccttcgt tgatccagat atcgctccag gtcgcgcagg ttactttgtc gccaaaccac
      601 atatggccca gttcatgtgc gacaagattg aagctgaatc cgccaatggt tgtcatggtc
      661 tggtgctcca tcccgccacc cagttgggta agacaatgac cgtacttttc atcgatgaaa
      721 ggataaagtc cgaataaatc ggagaaaagc tcaataaaac ctgccgtccg gtcaattccc
      781 acctggtata atggcaggca gtttacatga tcataaatat aattctggat gaggaccgaa
      841 tcttccgtcc ctgccggata agcataaatg ttgtattcct ggtaatcggc tacggcaaag
      901 gaaatcaggt aataatcaat gggataataa cttttccatt cgtaacggag ttttccgccg
      961 ggtaccggcg taacggcagt cagcagtccc tgtgaacccg ccttgttttc ggcgctcgtc
     1021 gtcagaaata cccaaaccga gtcggcatcg tcggtgagat cctgcttcgt cggccaccag
     1081 tctttcgcgg cataaggttc agataaggtc caggtaacgt ttttttcaaa tggcgtgctg
     1141 taagccgtcg tcacccctga gaaaaagccg ccggttggag gtgtaccatg ataacaaatt
     1201 tttgtcgtaa aataaacgcc gtttgggatt ggttcgatcg gaaccaggat caggtcatta
     1261 ttgtggacga acgaatgctg gataccgttg acatagaccg tatctaccgt catctcgtca
     1321 ataagttcat agacaatggt atccatcagg gtaccggtca ctatggcctt tgtggtaaca
     1381 cttccgctta cggctaccgt atcgttttcc acattcaggt ccaggaaata aaacgtaaca
     1441 tcgtattttt ccagcaatgg atttgtcaca acgggcgcat aatattcatt cgcagccatt
     1501 gccgctgatt tgacatgata acagtaaggt gcatcatcct gttcggcatg attgtactga
     1561 gccatcagaa ttcctgttaa cgggatcaga atgaaaagaa aaaccagtct ttttaacata
     1621 gaaaaaataa tttaaccgga aggtcgtgaa actataacta actttgtata agcgaacaaa
     1681 gataatattt atttgttcgt taccgttagg gttctatacc ctgaaaggct tgcatctcat
     1741 gacagaagac aactattcca tattgatccg taaactcgat gaatttatcc ggaagtttta
     1801 ccgaaaccgg ctgatcagag gagccatatt ttatgtggcc attgtaacga tagcctatct
     1861 cctcataacg ctcaccgagt tttttggacg gttcagcccc ggcatccgga ctctcttttt
     1921 ttattcattt ctggtccttt ctgccggttt tctgtggtat tatatcctgg tgcccgttat
     1981 ccggttgctg ggattcggaa agatcatttc tcacgaagag gcggcggaaa ttatcggcaa
     2041 acatttcccc gagatcagtg ataagctcct gaatacttta cagcttaaaa agctgcagga
     2101 acaccatacc gatgagtttc caatcgagct tatccgcgcc ggcatcgacc agaaaattga
     2161 aaaactgaga ccggtgcctt ttgccctggc catcgatttc cggaagaacg gccgttatct
     2221 ccggtatgcc ctgcctccgg tcatcattat tatcctgttg ctgatcatgt cgccccgtgt
     2281 gatcaccgaa ccgtcggaaa ggatcgtaaa gtatcagacg gtttatttgc cccccctgcc
     2341 gtttaccgtt caaatcctca acgaaaaact ggaatctttt cagcaggaag attttctgct
     2401 ggaattaaaa gccgaggggg aagaattacc ggccgaattg tttgttgaaa ccggaggagt
     2461 ccgctacagg atggaaaggt caagcccggt cctttttcgc tataatttcc gcaatctgca
     2521 ggaaaccaaa ttgtttatca tcagtaatga ccttttcaaa tcggaagaat accaactgac
     2581 tgtttacccg aaacccattg tgctcagctt tgagatttca ctggattatc ctgtttatac
     2641 cggaaaatca gatgaaaaat ctgaaaatac gggcgatctg attgttcccg aggggacc
//
LOCUS       DAOQXS010000415         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41433_length_2690_cov_3.354839, whole genome shotgun sequence.
ACCESSION   DAOQXS010000415 DAOQXS010000000
VERSION     DAOQXS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41433_length_2690_cov_3.354839"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKM34_10520"
     CDS             complement(<1..812)
                     /locus_tag="PKM34_10520"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNQ84067.1"
                     /translation="MISEIKIERIDENRLLIPKSGNMKVPGIIYANENIQRLLKSDES
                     ARQVINVAQLPGIVNYSLAMPDVHWGYGFPIGGVAAFDLSEGVVSPGGVGYDINCGCR
                     LMATKLTLFDVKDKIQDLTLALYNHIPTGVGSTGHVKLAGKDQKKVLIEGANWAIVNG
                     FGSPEDLETTEDGGVMAGADPEKVSARALERGKQQLGTLGAGNHFLEVEYVQEIFDEK
                     AASAFGLQKDQIVVMIHTGSRGLGYQICDDYLALMVKHAHETGIELPDRQLA"
     gene            complement(802..1236)
                     /locus_tag="PKM34_10525"
     CDS             complement(802..1236)
                     /locus_tag="PKM34_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNQ84068.1"
                     /translation="MKKYKLIDHTADIGCEIYGGTRKELFANSVVALFELMLGRKTIS
                     RTNENPALETKSLTVEGEDLEDLFINFLREVLYLFNGEGWVVVSCQPLQLTTKHIVAR
                     LTGEPYISTKHQVKMEIKAVTYHGLRVKKTKTGWVGRVIYDI"
     gene            1611..2357
                     /locus_tag="PKM34_10530"
     CDS             1611..2357
                     /locus_tag="PKM34_10530"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNQ84069.1"
                     /translation="MKVESGFYEGLSQEDLARLAVDMLHRTIVHHIFWFKEVEHQMGT
                     EEALKVLSDAYTKSYNIQMNRLAKIFGFEMVNGVPKLLLDMPKDKLLDVLTNLGANWL
                     ANDGIWFQAVEFKHGMNDAKRCNDTCWAKFSPFEAWSIKRYLNLEDKPGLEGLKKALQ
                     LRMYARINVQSIMDESPTSIVFQMNDCRVQSARKRQGLDDYPCKSAGLVEYGSFARAI
                     DSRIRTECVGCPPDAHPDEWFCAWRFILEE"
BASE COUNT          689 a          652 c          646 g          703 t
ORIGIN      
        1 caggccagtt gccggtcggg caactcgatc cctgtttcgt gcgcgtgctt gaccatcaac
       61 gccagataat catcgcaaat ctggtagccc aaaccgcgcg aaccggtgtg aatcatgacg
      121 acgatctgat ctttctgcag gccaaaggct gaggctgctt tttcatcaaa tatttcctga
      181 acgtactcga cttccagaaa atgattaccg gcgccgagcg tgcccaactg ctgtttgccc
      241 cgctccaggg cgcgcgcgga tactttctcg ggatcggctc cggccatgac gccgccatct
      301 tccgttgtct ccaaatcttc cggtgatccg aaaccgttga caatcgccca gttggcgcct
      361 tcgattaaga cctttttctg atctttaccc gccaatttaa catgacccgt cgagccgacg
      421 ccggttggta tatgattata caatgccagc gtcaggtcct ggattttatc cttaacatcg
      481 aaaagcgtaa gtttggtggc catcagacgg caaccgcaat tgatgtcgta accgacgccg
      541 cccggcgaaa cgacgccttc agacagatca aaggccgcca cgcctccgat ggggaaacca
      601 tacccccagt ggacatcggg catcgccaga gaataattaa caataccggg aagctgagca
      661 acgttgatca cctgccgggc gctttcgtcg ctctttaaga ggcgctgaat attttcgttg
      721 gcataaataa ttccgggaac cttcatgttt ccgctcttgg gaatcagcaa acggttttcg
      781 tctattcttt caatcttaat ttcagatatc atatatgacc cttcccaccc atccggtttt
      841 cgtcttttta accctcagac cgtggtatgt aactgctttg atttccattt tcacctgatg
      901 cttcgtgctg atataaggtt ctcccgtcag tcgggcaacg atgtgtttgg tggttagctg
      961 taaaggttga cagcttacca ccacccaccc ttcaccgttg aataagtaga ggacttcccg
     1021 caggaaattg ataaagaggt cttctaaatc ttcgccttcc acggtcaggc ttttcgtttc
     1081 caaggccgga ttttcatttg tcctggaaat cgttttccgt cccagcatca attcaaataa
     1141 cgcgacgacg ctattggcaa aaagttcctt tcttgtaccg ccgtatattt cgcagccgat
     1201 atcggcggta tggtcaataa gcttgtattt tttcattata taagtatagc tgaatatcac
     1261 ggaatgaaca agtgcggagg ctgctcacaa aatggtcgtc gtgccgggca atcgttcccg
     1321 gcgcgtgagt aataccattc ttcttttttg ttgccatatg cgacactttg gtaagtaatt
     1381 gtcaaatata atatttattt ttgtcatatc tggcaagcat ttggtgtcat cgccgtaagc
     1441 aattgattta cctgcttatt aatttggcat gatccctgct aaaattggaa gcaggagata
     1501 gcaggaagag gtaagaataa gtacacaacc gcaaacgtcg ctggtcgact cccaccagtt
     1561 tgcgccgggc ggcaaatgta tttgtaaatt tttaatcaag gaggaaaagc atgaaagttg
     1621 aatccggttt ttatgaagga ctatcacagg aagacctggc cagacttgcg gtagacatgc
     1681 tgcacaggac catagtacat cacattttct ggtttaagga agttgagcac cagatgggaa
     1741 ctgaggaagc cctaaaggtc ttgagcgatg cctataccaa aagctacaat attcaaatga
     1801 acaggctggc caagatattc ggctttgaga tggttaatgg cgtgcctaag cttttgctgg
     1861 acatgcccaa agataaatta ttggacgttc tcaccaatct cggcgccaac tggctggcca
     1921 atgatgggat ctggttccag gcggtagaat tcaaacacgg aatgaatgat gcaaaacgct
     1981 gcaatgacac ctgctgggcc aaattttccc cgtttgaagc ctggtcgatc aagcgttacc
     2041 tcaacctgga ggataaaccg ggcctggagg gactaaaaaa agctctgcag ctcaggatgt
     2101 atgcccgcat caatgtacag tcgattatgg atgaaagccc caccagcatt gttttccaga
     2161 tgaacgattg tcgagtccag tccgcccgca aacggcaggg tttggatgat tatccctgta
     2221 aatcagccgg tttggttgaa tacggcagtt ttgccagggc cattgattcc agaatccgca
     2281 ccgagtgtgt agggtgcccg cccgatgccc atcccgatga atggttctgc gcctggaggt
     2341 tcatcctgga agaataacga ggcgattgta ttgattcgaa gaatgaagtt aagaaagtaa
     2401 tcctgatgtt cggctaattc caaaggttgc attttgatag tcgctacgct cggtggtatt
     2461 gccggagcca aaagaatgac ccagatcgcc gggaagaccg gtttgggacg ggagagtttg
     2521 tacaaagcat tatcagcggc gggtaatccg gaattttcaa cagttctgaa agttgtgcaa
     2581 gccctcggtc tacaattaca cgcccgatcc ggaaaaacac gaaaagcggc atagtaagaa
     2641 gtccatttcg ctgtcattgc gagcaaagcg aagcaatctc atgatttata
//
LOCUS       DAOQXS010000416         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41489_length_2688_cov_3.514242, whole genome shotgun sequence.
ACCESSION   DAOQXS010000416 DAOQXS010000000
VERSION     DAOQXS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41489_length_2688_cov_3.514242"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKM34_10535"
     CDS             complement(<1..816)
                     /locus_tag="PKM34_10535"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNQ84070.1"
                     /translation="MKKLFFAILLVLLVNAGFSQARIQVIHNSADAALETVDIWLDET
                     LLLDNFSFRTASAFTDAVPGNVSIVVAEADSQNPDNPLWSGNVELTDNETYVIVAEGM
                     ISQTGYNPYVPFDLAVYSGAREIATSEEMTDMITHHGSTDAPDLDINEIAIGLGTIVD
                     NIGYSEFDGYLELATKDFMFDVTEAAGSPLIDTYTAYFRILGFKGMAITVLTSGFLDP
                     AGNSNGPAFGLWVATAAGGDLIELPPYNPTARVQIIHNSADAAASTVDVWMNDT"
     gene            952..1389
                     /locus_tag="PKM34_10540"
     CDS             952..1389
                     /locus_tag="PKM34_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNQ84071.1"
                     /translation="MGEIKTKPTESDVKLFIDSVENEKKRNDSYAILEMMSEVTGEKP
                     VLWGTSLIGFGKYHYKSKSGQEGDWFLTGFSPRKQNISLYLMAGYVRGEDEVFDDLMN
                     KLGKYKTGAGCVYINKLEDINVLYLKELIRLSVNFLKNKYPEI"
     gene            1465..>2688
                     /gene="nhaA"
                     /locus_tag="PKM34_10545"
     CDS             1465..>2688
                     /gene="nhaA"
                     /locus_tag="PKM34_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNQ84072.1"
                     /translation="MKDREKFHFSNFNISSYIREESLGGFLLLLTAVIAVIWANSGFA
                     DAYDKLWHEIKLGISFGDFQMKYSLQHWINDGLMAVFFFMVGLEIKREVIAGELSTLK
                     KASLPIAAAAGGMLFPALIYVAFNAGNPEHLSGWGVPMATDIAFALGILSLLGSRVNI
                     NLKIFLTALAIADDLGAILVIALFYTDTINIMQLVNAGIFLVILTVASRLGVRRTSFY
                     AIVGFLGVWISFMFSGVHATIAGVLIALTIPVKTKITEKEYVDELCVLVDKFERENPN
                     DKDLLTQRQSHLVSKIGKLSDDANTPLQKLEHALHPVTAYIILPLFALANAGVKIEGN
                     ILDMMFHPVALGIIAGLAVGKILGISLFSRLMVRLKLAELPEGVSWRQVYGAGMLAGI
                     GFTMSIFISGLAFKSE"
BASE COUNT          714 a          590 c          660 g          724 t
ORIGIN      
        1 ggtatcgttc atccagacat cgacagtcga agctgctgcg tcggcagagt tatggatgat
       61 ctgcacacgg gcggtaggat tatagggagg aagctcgatc aggtcaccgc cggcagccgt
      121 ggcaacccac aggccgaaag ccgggccgtt gctgttgccg gccggatcga gaaagccgga
      181 agtcagcacg gttatggcca ttcctttgaa tccaaggatg cggaaatagg ccgtataggt
      241 gtcgatcagc gggcttccgg ccgcttccgt cacatcgaac atgaagtctt tggtagccag
      301 ttccaggtac ccgtcaaatt cggaatatcc gatattgtcg acgatagtgc caagtccaat
      361 ggctatttca ttgatatcca ggtcaggggc gtcggtggaa ccatgatggg tgatcatatc
      421 ggtcatctct tcggatgtcg ctatctcccg ggctccactg taaacagcca gatcgaacgg
      481 gacatacgga ttgtaaccgg tctggctgat catcccttcg gccactataa cataagtttc
      541 attatcagtc agttcaacgt ttcccgacca gagggggttg tcgggattct ggctgtcggc
      601 ttcggccacg acaatcgaca cattaccggg aacggcatct gtgaacgcag aggctgtccg
      661 gaatgaaaaa ttatccagca atagcgtttc atccagccag atatcaacag tttcgagtgc
      721 agcgtcggcc gagttatgga tgacctgtat cctggcctgt gaaaatcctg catttacaag
      781 cagtacgagt agaatagcaa aaaatagttt tttcatttta taacggatta taacatttca
      841 aatatcttca aaatttattt ttacaaatat aggataagtc ggaatgcaat ttttctccat
      901 atttgaacaa tgactaacaa atttggttta ataacataca attttaccgt tatgggagaa
      961 ataaagacaa aacctaccga atcggatgta aaactattta ttgactcggt agaaaatgaa
     1021 aaaaaaagga acgacagtta cgcgatcctg gaaatgatga gcgaagttac cggtgagaag
     1081 cccgtattgt ggggcacttc gctgatcgga ttcggaaaat accattacaa atccaagagc
     1141 ggccaggaag gcgactggtt cctgaccggt ttttcacccc ggaagcagaa catatcactg
     1201 tacctcatgg ccggttatgt caggggcgag gatgaagtat ttgacgacct gatgaataaa
     1261 ctgggcaaat acaaaacagg ggccggttgt gtttatatca acaagctgga ggatatcaac
     1321 gtgttgtatt tgaaagaatt gatccggttg tcggtcaatt ttctcaaaaa caaatatcct
     1381 gaaatataat atggatggtt ttacatcccg ccaaatcaga attcttcatt atttttgata
     1441 gtaaagatga tgttttataa aaagatgaag gatcgcgaaa agttccattt cagcaatttt
     1501 aatatttcca gttacatccg tgaagaatcg ctgggtggat tccttttact tcttactgct
     1561 gtaatcgccg ttatctgggc caattcggga tttgctgatg cttatgataa attatggcat
     1621 gagataaaac tgggaatatc tttcggggat tttcaaatga aatattcact tcagcactgg
     1681 atcaacgacg ggctgatggc tgtgttcttc tttatggtcg ggctggagat aaaacgggaa
     1741 gtgattgctg gagaattatc cacattaaaa aaagcatctt tgcccatagc cgcagccgca
     1801 ggcgggatgc tattccctgc gctgatctat gttgcattca atgccggcaa tccggaacac
     1861 ctctccggct ggggcgttcc catggctacc gatattgctt tcgcactcgg gatcctttcc
     1921 cttctcggga gcagggtgaa catcaacctg aagattttcc tcactgccct tgccattgcc
     1981 gacgacctgg gagctatcct ggtcatcgcc ctgttctata ccgatacgat aaatatcatg
     2041 caactggtca acgccgggat tttcctggtc attttaaccg ttgccagccg gctcggggta
     2101 aggaggacct cattctacgc catagtcgga ttcctcggag tttggatttc gtttatgttc
     2161 tccggtgtac atgcgacgat agccggtgtt ctgattgccc tgaccattcc ggttaaaacg
     2221 aaaattacag agaaggaata cgtcgacgaa ttgtgtgtcc tggtcgataa gtttgaacgg
     2281 gaaaatccta atgataagga tctgctcacg caaaggcagt cgcacctggt gtcaaaaatc
     2341 ggcaagctca gcgatgatgc aaatactccg cttcagaaac tggaacacgc tcttcaccct
     2401 gtaacggcct atatcatcct tcccttattt gctttggcca atgcaggggt gaaaatcgaa
     2461 ggaaacatcc ttgacatgat gtttcatccg gttgcccttg gcattatagc cggtctggca
     2521 gtgggtaaga ttttagggat ttcattgttt tcccggctga tggtcaggct gaaactggcc
     2581 gaattacccg aaggtgtttc atggagacag gtttatggcg caggaatgct ggccgggata
     2641 gggttcacta tgtctatctt tatttcaggc ctggctttta aaagcgaa
//
LOCUS       DAOQXS010000417         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41825_length_2672_cov_4.840275, whole genome shotgun sequence.
ACCESSION   DAOQXS010000417 DAOQXS010000000
VERSION     DAOQXS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41825_length_2672_cov_4.840275"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKM34_10550"
     CDS             complement(<1..418)
                     /locus_tag="PKM34_10550"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HNQ84073.1"
                     /translation="MKYEFKYVVPVSQLDALREAIKPYVEVDPYAEATHLNEYTVRSI
                     YYDTARFDYFFEKVDGYKMRKKIRIRGYNDQKGGDTVFMEIKRKFKEPIEKDREKLTF
                     EVMKRLMAGEGAAAYGDVPDDHGVNGAGKFLYHVYRN"
     gene            complement(421..1479)
                     /locus_tag="PKM34_10555"
     CDS             complement(421..1479)
                     /locus_tag="PKM34_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84074.1"
                     /translation="MRISRRHILIILFVLIVITIFLPIPVRYSFIATAKIYPFREWKL
                     MRGTEEGFWSQTFNYETDALGDFKNYRFERGDIAELEINEKLVFDMPVSQHDTIAYIR
                     SFFIENEITRLENLRDVEAANKDVVSTGEKRPLVEQAQRQYNYALQQLDLEKKNFARQ
                     EKLFRDSVITPAEFDLYENTLKLAEINAQVAFEALTSLQTGEKDPVVNLSEQKITSYE
                     KEVERLKTQKAQYTIVTPISGILSYNTEIGGIIKVSDSEKLLLKIPVAYEHSSYLNNL
                     YKVTFSTPDNKITVNASFKGFDESVNQIQNKQFVIARAVTTGPTTGIYPGMVVQCRIY
                     CDKVYLLEYLKRNFSVSF"
     gene            complement(1567..2250)
                     /locus_tag="PKM34_10560"
     CDS             complement(1567..2250)
                     /locus_tag="PKM34_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ84075.1"
                     /translation="MITAIALDDEPPALRIVENFCGKVDFIRLEKSFTHPHEALKHLE
                     KFPADLLFLDIRMPSMSGVEFAKKVPGELMVIFTTAYSEYAVEGFNLNAVDYLLKPFT
                     FERFMQAVNKAGDYYRFLHQSEQALQQYLFVRADYSLVRIILDEILYIEGLDDYLRIF
                     LTNRKPVVARMTMKTMLEKLPADRFARVHRSYIVPVSRIDKVRNKIIFIGDKQIPIGN
                     SYEKGIFRE"
     gene            complement(2247..>2672)
                     /locus_tag="PKM34_10565"
     CDS             complement(2247..>2672)
                     /locus_tag="PKM34_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ84076.1"
                     /translation="SEASKEMVPLDREIGYITDYIELQRIRFGETVKIDYHPCETAPG
                     KVIAPLLLIPFIENAFKYGVNPEADSNITVRVILYENDLHLHVFNYKVQDRKEDEVPG
                     GVGVNNARKRLGLIYPGKHKLDINENEFEYKVDLYIDLG"
BASE COUNT          638 a          692 c          603 g          739 t
ORIGIN      
        1 tgttacggta aacatgatag agaaacttgc cggccccgtt gaccccgtga tcatcgggca
       61 catcgccgta agcagccgct ccttcgcccg ccatcaggcg tttcatgact tcaaaggtca
      121 gcttctcccg gtccttctcg atcggctcct tgaatttcct tttgatctcc ataaagaccg
      181 tatctccgcc tttttgatca ttataccccc tgatccggat cttcttcctc attttatatc
      241 cgtcgacctt ttcaaaaaaa taatcgaacc tggccgtatc ataataaatc gacctgaccg
      301 tgtattcgtt aagatgggtt gcttcggcat acggatccac ctccacgtaa ggctttatcg
      361 cctccctgag cgcatcgagc tggctcaccg gcaccacata tttaaactcg tacttcatga
      421 tcagaaagat acggaaaaat tccgtttgag gtactccagc aggtaaactt tatcgcaata
      481 aatacggcat tgcaccacca tccccgggta aatccctgtc gttggcccgg tcgtcaccgc
      541 acgggctatc acaaactgct tgttctggat ctggttcacg ctctcgtcga agcccttaaa
      601 ggaggcattg accgttattt tattgtcggg agtcgaaaaa gtgactttgt aaaggttgtt
      661 cagataagaa gagtgttcgt atgcaacagg gatcttcaac aaaagctttt cgctgtcgct
      721 cacctttatg atcccgccga tctccgtatt gtagctcagg atgccggata tgggggtaac
      781 gatcgtgtat tgcgctttct gtgttttcaa acgttcaacc tctttttcgt atgaggtgat
      841 cttctgttcc gacaggttta cgaccgggtc tttttccccg gtctgcagcg atgtcagggc
      901 ttcaaaagct acctgtgcat tgatctcggc cagtttcagg gtgttttcat acaagtcgaa
      961 ctccgcggga gtgatcacgc tgtcgcggaa aagcttttcc tgccgggcaa aattcttctt
     1021 ctccaggtcg agctgctgca aagcgtagtt gtattggcgc tgtgcctgct ccaccagcgg
     1081 tcgcttctcg ccggtcgaca ccacatcctt attggcggcc tcaacatcgc gaaggttttc
     1141 aagccgggtg atctcatttt caataaagaa agaacggata tatgcgatgg tatcatgctg
     1201 gctcacaggc atatcgaaaa ccagcttctc attgatctcc agttcggcaa tatcaccccg
     1261 ctcaaaccgg taattcttga aatcgcccag ggcgtcggtc tcgtaattga aagtctggct
     1321 ccagaatccc tcttccgttc cgcgcatcag tttccattcc ctgaacgggt aaatctttgc
     1381 cgtggcaata aaactgtacc tgaccggaat agggagaaag atggtgatga cgatcagcac
     1441 aaagagaatg atgagaatat gacggcggga aatgcgcatc ctgaatatta aaactggggc
     1501 aaagttaaat tattattaat ttctttacga tttgattatt tttccctttt cccatttcct
     1561 ttttaactat tccctgaaaa tccccttttc atagctattt ccgatcggta tctgcttatc
     1621 tccgataaaa atgattttgt tcctcacctt atcgatcctg gaaacgggga cgatatagga
     1681 ccggtgcacc ctggcgaacc ggtcggcggg cagtttctcc agcatcgttt tcatcgtcat
     1741 ccgggcaacg accggcttgc ggttcgtgag gaagatcctc aggtaatcat ccaatccttc
     1801 aatatacaga atctcatcca gtatgatcct gaccaggctg taatcggccc tgacaaagag
     1861 atattgctgc agggcctgtt ccgactggtg aaggaaccgg taatagtctc cggctttgtt
     1921 cactgcctgc atgaaacgct cgaatgtaaa gggcttgagc agataatcga ccgcattgag
     1981 gttgaaacct tcaacggcat actcactgta cgcagtggta aaaatgacca tcagttctcc
     2041 gggcactttt ttagcaaatt ccacgcccga catggagggc atccggatat cgagaaagag
     2101 caggtcggcc gggaacttct ccaggtgctt caacgcctcg tgaggatggg taaagctctt
     2161 ctccaggcgg ataaaatcaa cctttccgca gaagttttcc acgatcctca gtgcgggagg
     2221 ctcatcatcg agtgcaatgg cagttatcat cccaggtcaa tatatagatc aactttatac
     2281 tcaaattcat tttcgttgat atccagcttg tgtttgcccg gatagatcag ccccagcctt
     2341 ttacgggcat tgttcacacc tactcccccg ggaacttcat cctctttccg gtcctggact
     2401 ttgtaattga aaacatgcag gtgaagatcg ttttcataga gaatgaccct gacagtgata
     2461 ttggaatcag cttccgggtt gacgccatat ttgaaggcgt tttcgatgaa cgggatcagc
     2521 agaagcggcg caattacctt acccggagcg gtttcacagg gatgataatc gattttaacc
     2581 gtttccccga agcggattct ttgcaactcg atataatcgg taatataccc gatctcccgg
     2641 tcgagcggca ccatctcctt actggcctcc ga
//
LOCUS       DAOQXS010000418         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_41859_length_2671_cov_2.978976, whole genome shotgun sequence.
ACCESSION   DAOQXS010000418 DAOQXS010000000
VERSION     DAOQXS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41859_length_2671_cov_2.978976"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PKM34_10570"
     CDS             <1..1584
                     /locus_tag="PKM34_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84077.1"
                     /translation="NTGTDPFITNGWTEHTYGECTGDYMKTNQSNYGNSDGSTTFWYY
                     NDGSPYAGNYNNNDGMYGIKLFFESRGYTVTSYYNQYIYGYLGNTLGFTFNQYMAEID
                     AGRPVLIQVDGHTMLGMGYNETGSIVYLHDTWDYSTHQMTWGGSYSGMAHYGVGVIQL
                     QVITNDPCSNISVIGGCGSGYSQTYTGGGSGQWYTTTANPCGYLTPGIEKIYSFVAPY
                     NGTYSIQVTAASGYVDYLWKSGSCASTGWNCIDDITSPGAYGAMTWSAGTTYYILLDD
                     ENSTTGTHTFYIDFPICYDCPSYNLNLAPSTSWQTHSSSHVSYGCKTYRISVTSGLPY
                     IFKTGCGNGATASYNTLLELYNSGCTLVASNDNGCESNRSSISWTANYTGYAYLKVKG
                     VGTASGSYTLAYINNSVPANRLVQNVTVLSGQSPCYDATQNVITAGSGTTFIVQSGGD
                     VSLIAGSSVLMLPGTLIQSGAYLLGSITTNGSYCYTQAAPGQEQPVIRTIELIPGVMA
                     IPVVGVESEEREQGTGNRE"
     gene            1627..2340
                     /locus_tag="PKM34_10575"
     CDS             1627..2340
                     /locus_tag="PKM34_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84078.1"
                     /translation="MKKNVILVLVLFMMAGLANAQWAVNGSHIYNTNAGNVGIGMNAP
                     TSLLDVAKSMTEPTISVRNLGSNGGATYRMYDQLSGADWKFKATTTGGFKIRDNAFSL
                     DVMIVEANSAANVIYINAAGNVGIGTAAPSEKLAVNGNIKCKQVEVSLAGWSDFVFED
                     DYDLMPLAELEAYINQHNHLPDVPSAEEVISNGNNLGEMDAILLQKIEELTLYVIELK
                     KENEALKQAIGIPAEKIVH"
     gene            2491..>2671
                     /locus_tag="PKM34_10580"
     CDS             2491..>2671
                     /locus_tag="PKM34_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84079.1"
                     /translation="MKRITPLIILMLMAALLIAQQSMPEGKYIADRYIDENGREVVII
                     IVPGTPPGDHREPAAI"
BASE COUNT          722 a          657 c          652 g          640 t
ORIGIN      
        1 aatacaggaa ccgatccctt catcaccaat ggatggaccg agcacaccta cggggaatgc
       61 accggcgatt acatgaaaac gaaccaatcc aattatggaa attcggatgg atcgacaact
      121 ttctggtatt ataatgatgg ttcgccatat gcaggcaatt acaacaacaa cgacgggatg
      181 tacgggatca agttgttttt tgaatcacga ggctatacgg tgacatccta ttacaaccag
      241 tatatttatg gctatctggg caacacactg ggcttcacct ttaaccagta tatggcggaa
      301 attgatgccg gacggccggt tctgatccag gtcgacggtc acacaatgct gggtatgggc
      361 tacaatgaga cgggcagcat agtttacctc catgacacat gggattattc cacccaccag
      421 atgacctggg gtggttctta ctccgggatg gcccattatg gtgtaggcgt gatccagttg
      481 caggttatca ccaacgaccc ctgctccaat atttccgtta tcggaggatg cggttcagga
      541 tattcccaga cttataccgg gggcggcagc ggccagtggt ataccactac cgccaatcct
      601 tgcggctacc tgactccggg gattgaaaag atatacagtt ttgttgcgcc atacaacggc
      661 acctatagta tccaggttac ggccgccagc ggttatgtcg actacctgtg gaaaagcggc
      721 tcctgtgcct caacaggatg gaactgtatc gatgatatca cctctccggg agcctatggc
      781 gccatgacat ggtcggccgg aaccacttac tatattttgc tcgatgacga aaacagcacc
      841 accgggactc atacctttta tatcgacttt ccgatctgct acgattgtcc ttcttataac
      901 ctgaatttag ctccatccac ttcgtggcag acccattctt cctctcatgt ttcatacgga
      961 tgcaagacct acaggatcag tgtaacctcg ggattgccct atatctttaa aacaggatgt
     1021 ggtaatgggg caaccgccag ttacaatacg ttactggaat tgtataattc cggatgtacg
     1081 ctggtggctt ccaatgataa cggatgcgaa tccaaccggt catccatctc atggacagcc
     1141 aactacaccg gatatgccta tctgaaagtt aaaggtgtag ggacagcttc gggttcatat
     1201 acactggctt atatcaataa ttccgtaccg gcaaaccggc tcgtacagaa tgtaacggtg
     1261 ctttcaggcc agtcgccttg ctatgatgcc acgcaaaatg tcatcactgc cggcagcggc
     1321 acaacattta tcgttcagag tggcggcgat gtcagcctga tcgcaggtag ttccgtcctg
     1381 atgcttccgg gcacgctcat tcagtcgggc gcatatcttc tgggtagtat caccaccaat
     1441 ggttcctatt gttacacgca ggccgccccc ggtcaggaac agcctgtcat ccgcacaata
     1501 gaactaattc ccggtgtgat ggcgattccg gtggtcggtg tggaaagcga agaaagggaa
     1561 cagggaactg ggaatagaga atagtgaata gtagaatctg ataataaaaa ataaatattc
     1621 taaaccatga aaaaaaatgt aattcttgtg ttagtcctgt tcatgatggc agggcttgcc
     1681 aatgcccagt gggcggttaa cggatctcat atttacaaca ccaatgccgg caacgtgggg
     1741 atcgggatga acgctcccac atcgcttctg gatgtggcaa aaagcatgac ggaaccaacg
     1801 atctctgtcc gcaacctggg aagcaacgga ggtgcgactt accggatgta cgaccagctc
     1861 agcggggccg actggaaatt caaagccaca accactggcg gattcaagat cagggataat
     1921 gcattcagtc ttgatgttat gattgtcgaa gccaacagtg ctgctaatgt gatctacatc
     1981 aatgctgccg gcaatgtcgg gatcggaacg gctgcaccat cggaaaaatt agcagtgaac
     2041 ggcaatatca aatgcaagca ggtggaggtt tcccttgcgg gatggagcga tttcgtattt
     2101 gaagatgact atgacctgat gccgctggcc gagctggaag cctatatcaa ccagcacaac
     2161 cacctgccgg atgtgccatc tgccgaagag gtcatcagca atggcaacaa cctgggcgaa
     2221 atggatgcca tcctcctgca aaagatcgaa gaactcaccc tctatgtgat cgagctgaaa
     2281 aaggagaatg aagcgctgaa acaagctatc ggcataccgg cagaaaaaat cgtccattga
     2341 gtcttttttt agccacaaag gcgcactaag gatgtcacaa aggcgcacaa agtgattaaa
     2401 tatctgatca atacctttgt gttactttgt gaagcccttt gtgttctttg tggttaaccc
     2461 ctattaatac aacttaatag gatcttcgca atgaaaagaa ttacccctct tattattttg
     2521 atgctgatgg cagccctgtt gattgcccag caatccatgc ccgaagggaa atatatcgcc
     2581 gaccggtata ttgatgaaaa cggccgggaa gtcgtcataa tcatcgtccc gggaacgccc
     2641 cccggcgacc accgcgaacc tgcagctatt c
//
LOCUS       DAOQXS010000419         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_42477_length_2645_cov_2.970270, whole genome shotgun sequence.
ACCESSION   DAOQXS010000419 DAOQXS010000000
VERSION     DAOQXS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42477_length_2645_cov_2.970270"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKM34_10585"
     CDS             complement(<1..272)
                     /locus_tag="PKM34_10585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNQ84080.1"
                     /translation="MSDEQNREFMEEAIRLAALNMDATHGGPFGCVIVKDGRIIASAY
                     NEVLRTNDPTAHAEIVAIRRACEKLNSYQLAGCEIYTSCEPCPMCLG"
     gene            complement(286..963)
                     /gene="aat"
                     /locus_tag="PKM34_10590"
     CDS             complement(286..963)
                     /gene="aat"
                     /locus_tag="PKM34_10590"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNQ84081.1"
                     /translation="MPVYLLNDEMRFPDPRLADTEGLLAVGGDLSPERLLLAYEKGIF
                     PWFSDYSPVLWWSPDPRMVLFPKEFKRSKSLRQTMRSRHFEIRFDSDFGSVITNCAST
                     RRKHEDGTWIVPEIIDAYSCLHAKGYAHSVETYREGKLVGGLYGVSLGKAFFGESMFY
                     LERDASKAALAALVERMTEWDFHFIDAQQNTEHLGRLGARPVQRSVFLHLLQEAVSYP
                     TVLGKWE"
     gene            complement(1067..1501)
                     /locus_tag="PKM34_10595"
     CDS             complement(1067..1501)
                     /locus_tag="PKM34_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ84082.1"
                     /translation="MLPALRKKSTFPSLFDEFFSGDVFPGFFLDFDKRMATPAVNIIE
                     SKDDYRIEVAAPGLDKADFRINIENNILTISAEKEEKQEEKDEKVMRREFNYSSFSRS
                     FTLPDSMDTEKISARHKDGVLNVILPKKEEAKEKPARTIKIS"
     gene            1854..>2645
                     /locus_tag="PKM34_10600"
     CDS             1854..>2645
                     /locus_tag="PKM34_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84083.1"
                     /translation="MRTIFRFMGIILLIFSGAGQTLAQWAANGNNIYNTNSGNVGIGT
                     NSPATLLHVAKSMTEPTITVQNLGGSGGATYVMLDNASGANWKFKATLSGGFKIRDHA
                     NLMDVIVIEPNSFANAIYISGSQSIGIGTSNPNSSALLDISSNVKGFLPPRMTLAQIE
                     GIPGPASGLMVFCTTDEKLYIYVNSAGLWKEVPYGTETINPNETCGSPTVRNHVAGTV
                     APVDKTVTYGTVLSDLSGIQQCWITQNLGADYQATTVNQTTEPPAG"
BASE COUNT          686 a          614 c          595 g          750 t
ORIGIN      
        1 cccaggcaca tggggcaggg ctcgcaactc gtataaattt cacaccctgc cagctggtat
       61 gagttcagtt tctcgcaggc gcggcgtata gccacaatct ccgcatgggc agtcggatcg
      121 ttggtccgga gcacttcatt ataagctgaa gcgatgatcc ggccgtcttt gacaatgaca
      181 catccgaaag ggccgccgtg ggtcgcatcc atattcaatg cggccagccg gatcgcctcc
      241 tccataaatt cccgattctg ttcgtcactc atcttgtaat attatttact cccatttccc
      301 taagaccgtt ggatagctga ccgcttcctg caacaggtgc agaaagacgg atctttgcac
      361 agggcgggcg cccagccggc ccagatgttc tgtattttgc tgggcatcaa taaaatgaaa
      421 gtcccattca gtcattcgtt caaccagggc agccagggca gcttttgatg catctctttc
      481 gaggtagaac atcgattccc cgaaaaaagc tttccccagt gacacgccgt acaacccacc
      541 gaccagtttg ccctccctgt aggtttccac actgtgggca taccctttcg catgcaggca
      601 cgaataggca tcgatgattt ccggaacgat ccaggtgccg tcttcatgtt ttcgccgggt
      661 tgaagcacag ttcgtgatga cactgccgaa gtctgagtcg aatcggattt caaaatgcct
      721 gctgcgcata gtctgacgca gactttttga tcttttgaac tcttttggaa aaagcaccat
      781 cctcgggtca ggcgaccacc ataagaccgg cgaatagtcg ctgaaccagg ggaatatacc
      841 cttctcgtat gccagtaaca acctttcggg cgacaggtca ccgccaacag ccagcaatcc
      901 ttcagtatca gccagcctgg gatcaggaaa gcgcatttca tcgttgagta aatataccgg
      961 cacgtacgtt tctttgagat gtcaaaagta caaaacaaat aaccggcgga tttacccgtc
     1021 ggttatttgt tttgtactcc gacgggtaaa tccgtcggtg gtgtgtttag gaaattttga
     1081 tggtcctggc cggtttttcc ttggcctctt cttttttcgg gagaatgacg ttcaggacgc
     1141 cgtccttgtg tcgggctgaa atcttttcag tatccatgct gtcgggcagc gtgaacgacc
     1201 gcgaaaatga ggagtaattg aactcccggc gcatcacttt ttcatctttt tcttcctgtt
     1261 tttcttcttt ttcagcgctg attgtcagga tgttgttttc gatattgatc ctgaaatccg
     1321 ctttatcgag cccgggcgcg gcgacctcaa tccggtaatc atctttgctt tcgataatgt
     1381 tgacggcagg tgtggccatc cttttgtcaa aatcaaggaa aaaaccgggg aaaacatcac
     1441 cgctaaagaa ctcatcaaac agactcggaa acgtgctctt ttttcttaat gctggtaaca
     1501 tggcttgttc ctcctttact ttaagttaat gtttaatttt taattcttaa ttcttaatta
     1561 tgaatgactt aaattaagca tttgaaaata tcaaagtttg ggccgaagga ataattagtc
     1621 aaatatatgc cataatggct gatatttaca aatgatctag attttaaaaa gacataatgg
     1681 catggttcta taattatttg acatttatat ttttatttct tatcttagtc acggtgaata
     1741 tttattcaat aatcccctct ttgccaaagt cacgattatt ggttaacgac gaccatccaa
     1801 atgattgatt ctgaaatgta ttcaattatt gttaaccata aacccaagtg attatgagaa
     1861 caatttttcg tttcatggga attattttac tgatcttttc cggagccggt caaaccctgg
     1921 cacaatgggc tgccaacggg aacaacatct acaataccaa ttccggtaat gtcgggattg
     1981 ggaccaattc tccggccact cttcttcatg tcgcaaaaag catgaccgag cccacgatta
     2041 cagtacagaa tttagggggt tcgggaggtg cgacgtatgt gatgcttgac aatgccagcg
     2101 gtgctaactg gaaattcaag gcaaccttat caggcggatt caagatccgg gatcatgcga
     2161 acctgatgga tgttatcgtc atagagccga acagttttgc caatgcgatt tatatcagcg
     2221 gttctcaatc tattggtatt gggacgtcaa atccaaatag ttctgctttg ttagatataa
     2281 gttcaaatgt gaaagggttt cttcctccaa ggatgacact tgcccagatc gaaggaatac
     2341 ccggacctgc atcaggatta atggtctttt gcacaacgga tgagaaactt tatatttatg
     2401 tgaatagtgc aggattatgg aaagaagtgc cttatggaac ggaaacgatt aatcccaatg
     2461 aaacttgtgg ttccccgact gtcaggaatc atgtggctgg aactgtagca cctgttgata
     2521 aaactgtgac gtacggaacc gtcttatcgg acttaagcgg tatacaacag tgctggataa
     2581 ctcagaactt gggcgcagat taccaggcta ccactgttaa ccagaccact gagcctccgg
     2641 ccggg
//
LOCUS       DAOQXS010000420         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_42629_length_2638_cov_3.173442, whole genome shotgun sequence.
ACCESSION   DAOQXS010000420 DAOQXS010000000
VERSION     DAOQXS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42629_length_2638_cov_3.173442"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2638)
                     /gene="porU"
                     /locus_tag="PKM34_10605"
     CDS             complement(<1..>2638)
                     /gene="porU"
                     /locus_tag="PKM34_10605"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNQ84084.1"
                     /translation="AREYAAHSVLREGDWFRIAVRQTGVHILTYQDLVDMGFNPGQSD
                     PRKISLFGNGNGMLPEPNSMDRPDDLTENAIFVAGEEDGRFDPDDYILFFGLGAVKVY
                     FNNFYDYFEHEVNFYTDETYYFLTQRPVNGKRIQEESAIYTEPTHEVTTFQDFVYYEK
                     EAVNLIKSGKIWYGEVFDQKLDYTFPFHLPGKVNEEPVILKLSLAGRTTSRTNFEVMV
                     SDQLQMELDVPSIVLNSQIYGRPILSGFEPFYTSNDSAVIRIIYNKTGTIDKGWLNYI
                     ELNYIRNLEHTGNQLLFRDMRIVGDSVVARYHLSGAAPGTTVWDITDPAMVLNIPVTA
                     ESNGVTITTSADSGREFVAFSPGAYYSPEFVERVENQDLHAMGSADYIIVAHPVFLEQ
                     ANRLADFHRQYSGMQVHIVTPRQIYNEFSSGAQDVSAIRDFVKMIYDRAGDGEEPRYL
                     LLFGDASYDYKRFLEIDNNFVPAYQSRESLKAAASFVTDDFFGCMDIDEGSNGSGTMD
                     LGVGRFPVHTLEQAKAMVDKTIQYMSPVRENFGPWRNSICFIGDDEDNNTHFGQAEGL
                     VKIADSLGPAFNVNKIYLDAYSQLKTPSGNRYPDVNKAIDQVVGEGALIVNYTGHGGE
                     IAWAEERILDIPAIQSYTNLNSLPTFVTATCEFSRYDDPGLVSAGELVFLNPEGAGIG
                     LFTTTRLAFSQSNYALNKRFYYESFIIDPLTGEYPRQGDLIRVAKTPSNQNIKNFVLL
                     GDPALMLAYPKMKVSALNVLNERSGRMTDTISAQSKVTIEGQVEDLQGNRLEGFNGTV
                     HVTVFDKPVLYTTRANDPSSKVADFYMQDKKIFRGEASVANGLFSFSFIVPVDISYQF
                     GDGKISYYALDTNNLIDAQGYDH"
BASE COUNT          666 a          693 c          593 g          686 t
ORIGIN      
        1 tatggtcata tccctgggca tctatcaggt tatttgtatc cagcgcataa taactgatct
       61 taccatcgcc gaactgatac gaaatatcga ccggtacaat gaaagaaaaa ctgaaaagac
      121 cgttggccac agatgcctct cccctgaaaa tctttttatc ctgcatataa aaatcggcta
      181 ctttactgct gggatcattc gcccgtgtcg tatagagcac cggtttgtca aatacggtca
      241 catgaacggt cccgttaaat ccttccagcc ggttgccctg cagatcttcc acctggcctt
      301 ctatcgttac tttcgattgt gcactgatag tgtctgtcat ccggcccgat cgctcgttga
      361 gcacattgag cgcactcacc ttcattttcg gataagccag catcaatgcc ggatccccca
      421 ggagcacaaa attcttgata ttctggttcg aaggcgtttt ggctacacgg atcagatcgc
      481 cttgcctggg gtattccccg gtcagagggt cgattatgaa tgattcgtaa taaaaccgct
      541 tattaagcgc gtaattggat tgtgaaaaag ccaggcgcgt cgtggtaaac aaaccgatcc
      601 cggcgccttc cggattgagg aaaaccagct caccggccga aaccagcccc gggtcgtcat
      661 accggctgaa ttcgcatgtt gccgtgacaa atgtgggtaa tgaattcaga ttggtgtatg
      721 attgaatggc cgggatatca agaattctct cttcggccca ggctatttcc ccgccgtgtc
      781 cggtataatt gactatgagg gcaccttcac cgactacctg gtcaatggct ttgtttacat
      841 ccggatagcg gtttccgctc ggtgttttta actgcgaata agcatccagg tagatcttgt
      901 tcacgttgaa ggcaggaccc agcgaatcgg caattttcac cagcccttca gcctggccga
      961 aatgggtatt gttatcttca tcatccccga taaaacagat ggaattccgc caggggccaa
     1021 aattctcgcg caccggcgac atatattgga tcgtcttatc caccatcgcc ttcgcctgtt
     1081 caagcgtgtg caccgggaaa cggcccacac ccaggtccat cgtccccgat ccgttactgc
     1141 cttcatcaat atccatgcaa ccgaaaaagt cgtctgtaac aaatgaagcg gcggctttca
     1201 atgattccct cgactggtat gccggcacaa agttgttgtc aatctccagg aatcttttat
     1261 agtcatagga agcatcgcca aacagcaata ggtaccgtgg ttcttcacca tccccggccc
     1321 tgtcgtatat cattttaaca aaatcccgta tggccgacac atcctgagcc cctgaagaaa
     1381 actcattgta aatctgccgg ggtgtcacga tatgcacctg catgccgctg tattgcctgt
     1441 ggaaatcggc cagccggttt gcctgttcga gaaacaccgg gtgagctaca atgatgtaat
     1501 ccgcactgcc cattgcgtgc aaatcctggt tttcgacccg ctcaacgaat tcgggcgaat
     1561 aataggcgcc tgggctgaat gcgacaaatt cccgcccgga atctgctgat gttgttatag
     1621 taaccccatt ggattccgct gttaccggaa tattcaggac catcgccgga tcagtgatat
     1681 cccaaacggt tgttccgggc gcggctccgg aaagatgata ccgggccaca acactgtcgc
     1741 cgacaatcct catatccctg aaaagcagct ggttcccggt gtgttcaaga ttccggatat
     1801 aatttaattc gatataattc agccaccctt tgtctattgt tcccgtttta ttgtatatta
     1861 tccggatgac agcagaatcg ttactggtat agaacggctc gaatcccgac aggatcggcc
     1921 ggccgtaaat ctgtgaattc agcacaatgg acggtacatc aagttccatt tgaagctggt
     1981 ctgaaaccat aacctcgaaa tttgtgcgtg atgtcgtcct gccggccagg ctaagcttca
     2041 gaataacagg ttcttcgttt actttgccgg gcaaatggaa tggaaaagtg taatcgagtt
     2101 tctggtcaaa gacttcgcca taccatattt tacccgattt tatcaggttc acagcctcct
     2161 tttcataata cacgaaatcc tggaaagtag tcacctcgtg tgtcggttcc gtatagatgg
     2221 cagactcttc ctggattctt ttcccgttca ccggccgctg agtcagaaaa taataggtct
     2281 cgtcggtata gaaattgacc tcatgctcaa aatagtcata gaaattgttg aaatagactt
     2341 tcacggctcc cagtccaaaa aacaggatat aatcatcagg gtcgaaccgc ccgtcttcct
     2401 ctccggcaac aaaaatggca ttctccgtca aatcatccgg cctgtccatg gaattaggtt
     2461 cggggagcat tccattgcca tttccaaata aagatatctt acgggggtcc gactggccgg
     2521 gattaaatcc catatcaaca aggtcctggt aagtaagaat atgcacaccg gtttgtctga
     2581 cagcaatccg gaaccaatct ccctcgcgca aaaccgaatg agcagcatat tccctggc
//
LOCUS       DAOQXS010000421         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_42723_length_2634_cov_4.029469, whole genome shotgun sequence.
ACCESSION   DAOQXS010000421 DAOQXS010000000
VERSION     DAOQXS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42723_length_2634_cov_4.029469"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2603
                     /locus_tag="PKM34_10610"
     CDS             <1..2603
                     /locus_tag="PKM34_10610"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNQ84085.1"
                     /translation="RRKYSPTLDIENFGLNNEDLSKSFEAGHEIGIGKATLKEITDHL
                     KETYCQSVGAEFFYIRNPEVKTWLQDKMERSRNSYPFTLDDRKYIYRDLARAVLFEKF
                     IHKKFPGQKRFSLEGAEALIPALDAIMEKGALLGTEEFIIGMPHRGRLNVLANILRKP
                     YSHIFSEFRGMKYEDETLLGDVKYHLGYTIERYTTAGKKMKLTLAPNPSHLEAVDPVV
                     EGIVRARADMDYKGDFNRIAPILIHGDASVAGQGIVYEVIQMSQLPGYKTGGTIHVVV
                     NNQIGFTTNYLDARSSTYCTDVAKVVQSPVFHVNGDDVEAIVATIQLAMEFRAKFQRD
                     VFLDILCYRKYGHNEGDEPRFTQPILYKIIETHPNPMEIYRRQLIEKGFLEASYFDEV
                     EAKIHETLEKDLEESRKIDKANIRSFLDNVWKDIRKATPADFDTSPETGVDLKKIKSI
                     GRRIAHVPEGENYFRKLVRLQESRLEMIEKTGQVDWAMGELLAYGTLLEEGFPVRISG
                     QDSIRGTFSHRHAVLKIEDSEKEYYPLAHISDRQARFEVYNSPLSEYGVLGFDYGYSL
                     ASPQTLTIWEAQFGDFANTAQVIFDQFICSAEDKWNVMNDLVILLPHGYEGQGPEHSS
                     ARIERFLILSAEYNMQVANVTTPANFFHLIRRQLHREFRKPLIVFTPKSLLRHARCIS
                     PLTDLASGSFREVIDDPDADQANIKKVVFCTGKVYYDLLEEKEKMKYNDTAIIRIEQL
                     HPFPYKQIRNILDKYLHTDDILWTQEEPANMGAWTFIQTKMQEFNLRLIARPASGSPA
                     TGSPEFHELQQRKVIEKTFSSCDCPKVAEECRMMCIGNRWKTFEEQIMKTGQHISSKS
                     VSAINKLQNK"
BASE COUNT          743 a          653 c          645 g          593 t
ORIGIN      
        1 cccgccggaa atactcccct accctcgata ttgaaaactt cggcctgaat aatgaagacc
       61 tctctaaatc atttgaagcc ggtcacgaaa ttggcatcgg taaggccacg ctgaaagaaa
      121 taactgacca cctcaaggag acatattgcc agtcggtagg cgcagaattc ttttacatcc
      181 gtaatcctga ggttaaaacc tggttgcagg acaagatgga aaggtcacgc aattcatacc
      241 catttactct cgacgacagg aagtacatct accgtgacct ggccagggcc gttctgttcg
      301 aaaaattcat tcacaagaag ttccccggtc aaaagcggtt ctccctggaa ggagcggaag
      361 cgctcattcc ggctttggat gccatcatgg aaaaaggagc cctgctgggt accgaagaat
      421 ttatcatcgg gatgcctcac aggggcaggc tgaatgtact ggccaacatt ctaaggaaac
      481 cttacagcca tatattcagc gaattccggg gaatgaaata tgaagatgaa accttgctgg
      541 gtgatgtcaa ataccatctg ggttatacga tcgaaagata taccacggca gggaaaaaaa
      601 tgaaactgac gctggcaccc aatccctccc acctggaagc cgtcgatccg gtagttgaag
      661 gtattgtcag ggccagggca gatatggatt acaaaggcga tttcaaccgg atagccccca
      721 tcctgatcca tggcgatgcc tctgtcgcgg gacagggcat tgtttacgaa gtcatccaga
      781 tgtcgcagtt acccggatac aagaccggag gaacgatcca tgtcgtggtt aacaatcaga
      841 tcggttttac gactaactat ctcgacgccc ggtcgagcac ctattgcacc gatgtggcca
      901 aagtggtcca gtcgcctgtt tttcatgtga acggcgacga tgtggaggcc atcgtggcaa
      961 ccatccagct ggcgatggaa ttccgggcca aattccagcg cgacgttttc ctcgacattt
     1021 tgtgttacag gaaatatggt cataatgaag gggatgaacc ccgttttaca cagcccatcc
     1081 tatataaaat cattgagacc catcccaacc cgatggagat ctatcgccgg cagctgatcg
     1141 aaaaagggtt cctggaagca tcttatttcg atgaggtgga ggccaaaatc cacgagacgc
     1201 tggaaaagga cctggaagaa tcgcggaaga tcgataaagc caatatccgg tcattcctcg
     1261 acaatgtctg gaaggatatc cgcaaagcca cacctgccga tttcgacact tcaccggaga
     1321 ccggtgttga tctgaagaaa ataaaatcca taggccgcag gatagcccat gtaccggagg
     1381 gggaaaacta cttccgcaag ctggtcagat tgcaggagag ccgtttggaa atgattgaaa
     1441 aaacaggcca ggtcgactgg gccatggggg aattactggc ctatggcacg ttactggaag
     1501 aaggattccc tgttcgtatc agcggccagg attcgatccg cgggactttt tcgcaccgtc
     1561 atgctgtttt aaagatcgaa gattcggaaa aagagtacta tcccctggcc catatcagtg
     1621 atcgccaggc ccgttttgag gtgtacaatt cgcccctttc ggaatacggc gttcttggct
     1681 tcgattacgg ttattcgctc gcttctcctc agacgctcac aatttgggaa gcccagttcg
     1741 gggattttgc caacacggcc caggtcattt tcgaccagtt catttgcagt gccgaagaca
     1801 aatggaatgt gatgaacgac ctggttattt tgctgccgca cggttatgaa ggccaggggc
     1861 ccgaacattc gagcgcccgg atcgagcggt tcctgatcct cagtgccgaa tacaatatgc
     1921 aagtcgccaa tgtgacgacc ccggccaatt tcttccacct tatccgacgc cagttacacc
     1981 gcgaatttcg caaaccgctt atcgtattta cacccaaaag cctgcttcgt catgcccggt
     2041 gcatatcccc actgaccgat ctggcatcgg gaagcttccg ggaagttatc gacgatccgg
     2101 atgccgatca ggctaatatt aagaaagtcg tgttctgcac cggtaaagtt tattatgatc
     2161 ttcttgaaga aaaagagaag atgaaataca atgatacggc catcatccgg attgaacaac
     2221 tgcatccttt cccttataaa caaatcagga acatccttga taaatacctt catacagatg
     2281 acattttgtg gacacaggaa gagccggcca atatgggtgc atggacattc atccagacaa
     2341 aaatgcagga attcaacctg cgactgatag cccggccggc cagtggaagc ccggcaaccg
     2401 gttcaccgga atttcacgaa cttcagcaac gcaaagtcat cgaaaaaaca ttcagcagtt
     2461 gcgattgtcc taaggtggct gaagaatgcc ggatgatgtg catcggcaac cgctggaaaa
     2521 catttgaaga gcaaatcatg aaaacaggtc agcacatttc aagcaaatcc gtttcagcca
     2581 taaacaaact gcaaaacaaa taggtttagc gtatgatcat agaggtaaaa gtgc
//
LOCUS       DAOQXS010000422         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_43090_length_2619_cov_4.344384, whole genome shotgun sequence.
ACCESSION   DAOQXS010000422 DAOQXS010000000
VERSION     DAOQXS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43090_length_2619_cov_4.344384"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKM34_10615"
     CDS             complement(<1..893)
                     /locus_tag="PKM34_10615"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNQ84086.1"
                     /translation="MILNFLKTTNPIQKFKKLLVGLSLVLLPTLLYPQGTQELNIYKM
                     INPDSPLPDSLYLIDIIKYIEDTLFLGGARISDIQYIGNPKGVGLFTDGADIGMNDGL
                     ILSNGWVGTTTIVDGGQNKTGAQAIPAHDSLGPYRAEPNGPPPLQDDDMDWLSGLFTP
                     NDPKPDTAYDPSIITFKFKPYYNSIKLEYVFASEEYLYEQPPFPPPPPPPIDVDMTGA
                     PVSDFMAVFIKRYPSEQNYNMIASLIGQDGDPSWIPVCVKTLNHTTPPGYYQPNYDRS
                     FIFDGASLPRQIFPFVSEGINV"
     gene            complement(971..2605)
                     /locus_tag="PKM34_10620"
     CDS             complement(971..2605)
                     /locus_tag="PKM34_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ84087.1"
                     /translation="MEGRNNRALYFFLALAALLYLPAFLINLNMVQLIRDEAIRAIVA
                     FEMLQRNDFITPTIGGEPYLMKPPLFNWILAGFFQLTGSYSETVIRMPVIITIALYAG
                     TIYLFLRKPFGNLFALVNALAFVTYGRIIFYESLHGLIDLTFSWLTYCFFMLSWHFFE
                     RKKFLALFLVAYAITAVSYLLKGLPSLYFTAVTLLILFIQGKKFRMLFNWRHFLGIGL
                     LILVVGGYYLLYFSRNAIEPERVFEVLTGEVTRRTVVRFGIWKTILQLFTYPFENIYH
                     FLPWSALVILFIRKGSLKLIRENKRLWYMLLVFVFNIIPYWTSPETYPRYILMLVPLF
                     MTILFFLYFEYSKQDNWNKRAVDYILGIILGLAAIAAVIFLIHPATRDLPHVLPVSIG
                     LFITMSAISFFYWRKATFRLFWVIIAILVFRIAFNYTIIPSWEKTHPVVATKKLASEL
                     AAETRDRDLYVYWNPSFKPDPYFQYRYNNEIFTFYLSTARGRITEVKTEKLPGALYLA
                     QWEHLRNMKYKVIRKIEPAWQVPVVLIEFSEPKVKF"
BASE COUNT          773 a          552 c          622 g          672 t
ORIGIN      
        1 acattaatgc cttctgatac aaaaggaaat atctggcgtg gcagggatgc cccgtcaaaa
       61 ataaaactcc tgtcgtagtt gggttggtag taccctggag gggtagtatg gttcagggtt
      121 ttgacacata ccggaatcca ggaaggatct ccatcctgcc cgataaggga agcaatcata
      181 ttgtagtttt gctcgctggg gtatctttta ataaaaacag ccataaaatc cgagacaggc
      241 gctccggtca tatcgacgtc tatcggcggt ggaggcggtg gtggaaaagg tggctgctcg
      301 tagagatatt cttcagaagc aaatacatat tccagtttta tagagttgta atagggtttg
      361 aacttaaaag taataataga agggtcatat gccgtatccg gtttcggatc gttgggagta
      421 aacaatccgg agagccagtc catatcatca tcctgtagcg gcggcggccc gttgggctca
      481 gcccggtaag gccccaggct gtcgtgggcg ggtatagcct gtgctccggt tttattctgg
      541 ccaccatcaa caatagtagt cgtacccacc cacccatttg agaggatcaa cccgtcattc
      601 atcccgatat ccgcaccatc ggtaaataaa ccgacacctt tgggatttcc gatatattga
      661 atgtcggaga tacgcgctcc gccaagaaac agcgtatctt caatatactt tataatatcg
      721 atcagataaa gggaatccgg cagcggagaa tcgggattga tcatcttata aatattcaat
      781 tcctgtgttc cctggggata gaggagtgtg ggcagtaaaa ccaggctcaa tcctactaaa
      841 agctttttaa acttttgaat cgggttggtg gtttttaaaa aattcaagat cattttgaaa
      901 atgttttctt tatgtgaaat aataaacctg taaagttaaa cacacttttt taatatgtgt
      961 atttcaaatg ctaaaatttg actttaggct ctgaaaattc aatcaaaaca accgggacct
     1021 gccaggctgg ctcaattttc ctgataactt tatatttcat gtttcgcaag tgttcccatt
     1081 gcgccaggta tagcgctccg ggcaatttct ccgttttcac ttccgtaatc cgtcccctgg
     1141 cagttgaaag atagaaagtg aatatctcgt tattatacct gtactgaaaa taaggatcgg
     1201 gtttgaatga gggattccaa tagacataca ggtccctgtc ccgggtttcc gcagccagct
     1261 ctgatgccag cttcttggta gccacaaccg ggtgggtctt ctcccatgaa gggataatcg
     1321 tgtagttaaa agctattctg aacacaagta ttgctattat aacccaaaat agccggaaag
     1381 ttgccttgcg ccagtaaaaa aaactgatcg ctgacattgt tatgaataaa ccgattgata
     1441 ccggcaaaac atgcggaaga tcacgcgttg cagggtgaat aagaaatata acagctgcaa
     1501 tggctgccaa tcctaagatt attcccaata tataatccac agctctttta ttccagttat
     1561 cctgttttga gtattcaaaa taaagaaaga aaaggatagt cataaataga ggcaccagca
     1621 tgaggatata acggggataa gtttcaggcg aggtccagta cgggatgata ttgaagacaa
     1681 aaacaagaag catataccac aggcgcttgt tctccctgat aagtttcagt gagcctttcc
     1741 tgataaatag aatgaccagg gctgaccagg gaaggaaatg ataaatgttt tcgaaggggt
     1801 aggtgaacag ttgaaggatg gttttccata tcccgaaacg cactacggtc cggcgtgtta
     1861 cttcaccggt cagcacctcg aatactcttt cgggttcaat cgcattgcgg ctgaaataaa
     1921 gcaggtaata tcctccgaca accaggatca gcaaaccgat ccccaggaaa tgccgccagt
     1981 tgaaaagcat cctgaacttt ttcccctgga tgaaaaggat taacagtgtg acagccgtga
     2041 aatacaggct tggcaatcct tttaacaggt atgaaacagc cgtgatggca taggcaacta
     2101 aaaacagggc caggaatttt tttctttcga agaaatgcca tgaaagcatg aaaaagcagt
     2161 aggtaagcca cgaaaaagtt aggtcgatta agccgtgcag cgattcgtaa aatattatcc
     2221 tgccataggt tacgaatgcc agcgcattga caagggcaaa taagttgccg aatggcttcc
     2281 gcaggaacag gtagatagtt ccggcataaa gagcgatcgt aatgatgacc ggcatccgga
     2341 tcaccgtctc cgaataactg cccgtgagct gaaagaagcc agccagtatc cagttgaaca
     2401 acgggggttt catcaggtaa ggttccccac cgatggtggg tgtgatgaaa tcgttgcgct
     2461 ggagcatttc gaacgccacg atggcgcgga tggcttcatc gcggatcagt tgaaccatgt
     2521 tcaggttgat caggaaagcc ggcaggtaga gtagagccgc cagtgccagg aagaaataaa
     2581 gtgccctgtt atttcttcct tccataattt atataccaa
//
LOCUS       DAOQXS010000423         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_43168_length_2616_cov_2.987895, whole genome shotgun sequence.
ACCESSION   DAOQXS010000423 DAOQXS010000000
VERSION     DAOQXS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43168_length_2616_cov_2.987895"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKM34_10625"
     CDS             complement(<1..386)
                     /locus_tag="PKM34_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84088.1"
                     /translation="MKRVIVISLSLLMIGPVLLSAQESQRKPATLTASEIFLAEGDQD
                     QNAGLPLTPHDASGNRDLNYPIDQIQPLAGAYMAQFYQTDLAQSFVPSADKICGAGIG
                     IYNFYNNTGDVTIMLYDNLPNAGGNLL"
     gene            550..2058
                     /locus_tag="PKM34_10630"
     CDS             550..2058
                     /locus_tag="PKM34_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNQ84089.1"
                     /translation="MKTRIKFQTTLTTLITLTILTTLTTLPLTAQIPVQIMISDARTA
                     AGNQLSRHGRVDRTLQMETREIRVNDTKMAYVFDLDPTGYLVIGASSGLPPVIAYSFE
                     SDFGQMDEQNPLYTLVKTDLASRWTFVRTPGYSSEGYYENRWESILNEVVLQKTAPLF
                     EQWPAAGDGWLKTNWTQNPPYNNFCPMDPVTGTRSYAGCPATAMAQIMNFHNTTNLTR
                     FDDTDDYYHNYAGRQYWIDDDAETVDFPSFPELNTYLDTLDAHYLAGTTLTDADKAAL
                     TFACGTAATQVYTSQGSGTFGVSQAHEAYLRFGCNSVALLDSDDADLFDRLKQNIKDT
                     LPAHLAVVNESWTTGHNVVVDGYNTDDYYHLNFGWGGSYNGWYLLPDEIPYELTVIEG
                     VVVDIMKKSPVGIDESADGSWQSAVRIYPNPATGLIFIELPANYFRFQAYPESKFQIS
                     IFGINGQKPVQYQNMEQSMAFDATGLAPGIYFVKVTDGSGLRVGKVAKFVKK"
     gene            complement(2003..2578)
                     /locus_tag="PKM34_10635"
     CDS             complement(2003..2578)
                     /locus_tag="PKM34_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84090.1"
                     /translation="MSCTKYSGTNLIEEVKWEYKFSSWSPYLKISYLYNEDQLVESDY
                     YTYQNDWTISAKDVAVLFDGRIDQVIMYNCLSGDCTDDYLFKIFYNSDRVSRIEEYYK
                     FGSTWDTTGLYQFRYDAHKNLTSERFTGEGEDNSIEYKYEAGPGNFLQIKIPGGGLIN
                     TGNYPVPSCTYRNVTSLQTLRPFQPLARFHQ"
BASE COUNT          716 a          618 c          588 g          694 t
ORIGIN      
        1 agcagatttc ccccggcatt cggcaggttg tcataaagca tgatagtaac atcgcccgta
       61 ttgttataaa aattgtaaat accaatgccg gccccgcaga ttttatctgc tgaggggaca
      121 aacgattggg caaggtctgt ctggtagaac tgggccatgt atgcgcctgc cagcggttga
      181 atctggtcga taggatagtt aagatcccgg ttgccgcttg catcatgcgg agtcaatggt
      241 aagccggcat tctggtcctg atctccttcg gcaaggaata tctcactggc cgtcaaggta
      301 gcgggttttc tttgactttc ctgtgctgag agtaaaaccg gaccgatcat cagcaaagaa
      361 agtgaaataa caattactct cttcataatt aagttgttaa ggtttgatta atttgtgaca
      421 aggaaactta tcgacgaaat taagatataa tcttcacaaa aacaaataaa tttgttctca
      481 agggttggaa acccttcttt gaaaaccgta tattcgaaaa ccaaaccaaa ataaatttca
      541 tcacctgata tgaaaacaag aatcaaattc caaaccaccc ttacaaccct tatcaccctt
      601 acaatcctta ccacccttac aacccttccc ttaacagcac agattccagt gcaaataatg
      661 atttcggacg cccggaccgc tgccggcaat cagctttccc gacacggccg ggttgatcgg
      721 acacttcaga tggagaccag ggaaatccgg gtgaacgata ccaaaatggc ttatgtcttt
      781 gacctggatc ccaccggtta cctggttatc ggggcctctt ccgggctgcc tccggtgatt
      841 gcctattcct ttgaaagtga tttcgggcaa atggatgagc agaatccgtt gtacaccctg
      901 gtgaagaccg atctggcaag ccgttggaca tttgtacgta ctcctggcta ttcatcggaa
      961 ggatactatg aaaatcgctg ggaatcaata ttaaatgagg tggtcctaca gaaaaccgct
     1021 ccgcttttcg agcaatggcc ggctgcggga gacggatggc ttaagaccaa ctggacccag
     1081 aatccgccgt acaacaattt ctgcccgatg gacccggtta ccggcacccg aagctatgcc
     1141 ggctgtccgg ctaccgccat ggcacagatc atgaacttcc acaacaccac caatctcacc
     1201 cggtttgatg atactgacga ctattaccac aattatgccg ggcggcagta ctggattgat
     1261 gatgatgccg aaacggtcga ttttccctcc tttccggaac tgaatactta cctcgacacc
     1321 cttgatgcgc attaccttgc cgggacgacc ctgacggatg ccgataaagc agcattgact
     1381 tttgcctgtg gtacggcagc gactcaggtt tacacatcgc agggttcggg cacttttggc
     1441 gtcagccagg cccatgaagc ctacctgcgc ttcggttgca attcggtggc cttgctcgat
     1501 tccgatgatg ccgatctgtt tgatcgattg aaacaaaata tcaaggatac gttgccggcc
     1561 cacctggccg tggttaacga atcgtggacg acgggccaca atgtggtggt cgatggctat
     1621 aataccgatg attattacca tctcaacttc ggatggggcg gcagttacaa tggctggtac
     1681 ctgttaccag atgaaatccc atacgaactc actgtgatcg aaggcgttgt tgtagatatt
     1741 atgaagaaat cgccggttgg aattgatgag tcggcagacg gcagttggca gtcggcagtc
     1801 aggatttatc ccaatccggc taccgggttg atcttcatcg aactgccagc aaattatttc
     1861 aggttccagg catatccgga gtccaaattt caaatctcca ttttcggtat aaacggccaa
     1921 aaacccgttc aataccagaa tatggaacaa tcaatggcct ttgatgccac agggctcgct
     1981 ccggggatct attttgttaa agttactgat ggaagcgggc taagggttgg aaaggtcgca
     2041 aagtttgtaa agaagtgaca tttctatagg tacaggaagg aaccgggtaa tttcccgtat
     2101 tgatcaggcc acctccaggg atctttattt gcaggaaatt tccaggtcct gcttcatatt
     2161 tatactcaat ggaattatcc tcgccttcgc cggtgaatct ttctgaagtg agatttttat
     2221 gcgcatcgta acggaactga tacaagcctg tcgtgtccca cgtggaacca aatttgtaat
     2281 attcttcaat tcttgatacc cgatcgctgt tataaaaaat cttgaacaga taatcatcag
     2341 tacaatcacc ggagaggcag ttatacataa taacctgatc gatcctgcca tcgaaaagaa
     2401 ccgcaacatc ttttgcactt atcgtccagt cattttgata ggtgtaataa tccgattcta
     2461 caagttgatc ctcattatac aggtaagata tctttagata gggtgaccat gaactgaatt
     2521 tgtattccca tttgacctct tcgataaggt ttgttcctga atacttagta catgacaaaa
     2581 attaataaca acttgaatat caataatata tgttaa
//
LOCUS       DAOQXS010000424         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_43283_length_2612_cov_2.728588, whole genome shotgun sequence.
ACCESSION   DAOQXS010000424 DAOQXS010000000
VERSION     DAOQXS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43283_length_2612_cov_2.728588"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKM34_10640"
     CDS             <1..527
                     /locus_tag="PKM34_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84091.1"
                     /translation="FSRDSRGDQLGVFGWHDTTGPRVEERHGHVFVNNLMYMSGENTS
                     PMLQTQQSSSLCERLSTPHLKSLNNNIFIRGDAAGENKIPTIRWTPYPDDDCSIDIFS
                     PEELSKIIPEFCTKCQYFENYQGPLFADIKNNDYRLVKAFPGIKAAAEIPDRIVRLSG
                     LKGTGKPFPGAFAP"
     gene            804..>2612
                     /locus_tag="PKM34_10645"
     CDS             804..>2612
                     /locus_tag="PKM34_10645"
                     /inference="COORDINATES: protein motif:HMM:NF020768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HNQ84092.1"
                     /translation="MKKKKNRLLVVLALALIMPATMMMVISIVGAGCNVVTPPRPEGP
                     CDIYAAAGFPCVAAHSSTRALYASYNGPLYQVIRQSDGKTLDIGVVKPTRDDPGGYAD
                     AAAQDKFCANTYCWISILYDQSGKGNNLIQSPRGGAGNPTAMGGFNSLPIADMAPVTL
                     MGHKVYGIFIEPGMGLRQDDPKGTAVDDQAEGQYWVINGHHYNGGCCFDYGNAEIDSR
                     DDGDGTMETTYYGNAVTWYYGQGPGPWIMTDQENNIVGCVTDSPANKYCPDLPTITWR
                     FVTATFDGEPGHWRTMGGDAQRGDLSIMFDGPRVQNEKATRPELISSYDPMRKQGAID
                     LGNGGDNGNWSQGTMYEGAMTAAGTFPTEETNQKVQANIVAAGYDVPRLSVAPANATD
                     MPPGLQTFSPGSSQNTTLTFTNTTGAPVKGLNLSLTLPGGWKAVVQNSTETSKSFPDQ
                     IEPGASVNATFTVTSGYKAFNGDLVGKASWKNTANGKSQTETAIEKVRNVSPVKINEF
                     RISDGSNTTNSFVELYNAGSSEVDISNWTLTMRPYQMPIFSSVNIPSGTKLASNGFYL
                     LGLSTSGLAVPAKAGESVIYVRSTTGMSAGDVIEIGE"
BASE COUNT          753 a          608 c          622 g          629 t
ORIGIN      
        1 aattcagccg cgatagccgt ggcgatcagc ttggcgtttt tggctggcac gatactacag
       61 gaccccgggt ggaggaacgc cacggccatg tctttgtcaa caatctgatg tatatgagcg
      121 gcgaaaacac ttcgccgatg ttacaaacac agcagtcatc gtccttgtgc gaaagactaa
      181 gcacacccca tctgaaaagc ctcaacaata atatattcat caggggagat gcggcaggag
      241 agaataaaat tccaacaata cggtggaccc cgtatcccga tgatgattgc agtatcgata
      301 tcttctcacc cgaagaactc agtaaaatta tccctgaatt ttgcacaaag tgtcagtatt
      361 ttgaaaatta ccagggtccg ttgtttgctg atataaaaaa caatgattac cggctggtaa
      421 aagcatttcc cggcattaag gctgctgcag aaatacctga tcgtatcgtt agattatccg
      481 gattgaaagg aacagggaaa cctttcccgg gtgcctttgc accgtaatag tgtccggcat
      541 taaaaggttc aggtaataaa gcaagtcaat tttgtctttg gtttcgttga tggcaaaaga
      601 aacactatcg acggccgatg ttttgatgtt atcactcctg aagacttcat gaatcggaaa
      661 gagcgacaac gatgttaaca atcaaataat tgatattttt tgtcatgtac taaacagatt
      721 atttattata attggaatgc ttagtataaa tgaatctatt taaaacctca tcatttaccg
      781 ttaaaaaccc aggagaataa aacatgaaaa agaaaaaaaa tcgtctctta gttgttcttg
      841 cattggctct cataatgcct gccaccatga tgatggtgat ttcaatagtt ggagcaggat
      901 gcaatgtagt tacaccaccc agaccggaag gtccatgtga catttatgca gcagcaggct
      961 tcccttgcgt ggctgcccac agcagtaccc gtgcgttata tgcatcatat aacggacctc
     1021 tttaccaggt catacgtcag tcggacggta aaaccctgga cataggtgta gtaaaaccta
     1081 ccagggacga tccgggcgga tatgctgacg cagccgccca ggataaattc tgtgccaata
     1141 cctactgctg gatctcaatt ctatatgacc agtcaggtaa aggcaacaat cttattcagt
     1201 caccccgtgg tggtgcaggt aaccccacgg ccatgggcgg atttaacagc ctgccgatcg
     1261 ccgacatggc tcctgttaca ctcatgggtc acaaagtcta tggcatcttc atcgagccgg
     1321 gtatgggcct tcgccaggac gatccgaagg gtactgcagt tgatgatcag gcagagggac
     1381 agtattgggt tattaacggt catcactata acggtggctg ttgcttcgat tacggcaacg
     1441 ctgagatcga cagccgcgat gacggcgacg gtacaatgga aaccacctat tatggcaacg
     1501 ccgttacgtg gtattatgga cagggacctg gtccatggat catgaccgac caggagaata
     1561 acattgtagg ttgtgtaacc gactcacctg ccaataagta ttgccctgat ctacctacaa
     1621 ttacctggcg ttttgttaca gccacatttg atggagaacc aggccactgg aggacaatgg
     1681 gcggcgacgc tcagagaggc gatctgtcaa taatgttcga cggaccccgc gtccaaaatg
     1741 aaaaagcgac gcgtccggaa ttaataagca gctatgatcc tatgcgcaag cagggagcta
     1801 tcgacctggg taacggtggc gataatggca actggtccca gggaactatg tacgaaggcg
     1861 caatgacagc tgcaggtaca ttcccgacag aagaaaccaa ccagaaggta caggccaata
     1921 tagtggccgc cggctacgat gtcccccgtc tgagcgtcgc tccggccaat gcaaccgaca
     1981 tgcctccggg tcttcagact ttctcaccgg gatcgtcaca gaacacgacc ttaacattta
     2041 caaatactac aggcgcaccg gtaaagggtc ttaacctgag ccttaccctt cccgggggat
     2101 ggaaggctgt tgttcaaaac tccacagaga cttcaaagag tttcccggat cagatagaac
     2161 caggggccag cgtaaatgca actttcactg taacttcagg atataaggct ttcaatggtg
     2221 accttgttgg caaagcctca tggaaaaata cagcaaacgg taaatctcaa accgagacag
     2281 caattgagaa agttcgcaat gtaagtccgg taaagataaa tgaattccgt atcagtgacg
     2341 gatccaatac tacgaactct ttcgttgagc tctataacgc cggcagcagt gaagttgata
     2401 tttcaaactg gacacttact atgcgtccat accagatgcc aatcttttca tccgtaaata
     2461 taccgtccgg gacaaaactg gcctcaaatg gtttctacct gttaggtttg tccacttccg
     2521 ggctggctgt tcctgcaaaa gccggtgaat cggtcattta tgtaagaagc acgacaggta
     2581 tgtcggcagg tgatgtaatt gaaatagggg aa
//
LOCUS       DAOQXS010000425         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_43302_length_2611_cov_3.880282, whole genome shotgun sequence.
ACCESSION   DAOQXS010000425 DAOQXS010000000
VERSION     DAOQXS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43302_length_2611_cov_3.880282"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PKM34_10650"
     CDS             complement(<1..1320)
                     /locus_tag="PKM34_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNQ84093.1"
                     /translation="MTNTFNLEWIDYAVMIIYFAFVLGIGWALKKYMKDATAFLEAGR
                     SMPAWVAGLAFISTNLGALEVMGMTGSGLKYGMLTTHFYWVGAIPAMVFLALFMMPFY
                     YGSKARSVPEYLKLRYDEKTRGLNAILFAVMTILASGISMYALAILMEKVLGWNFHLS
                     LILSAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPVVFLSLKDVGGWQGLQEQLAP
                     IATEKGFAPDTWTTTWKYTGSPGSNPMGVEWYSIFAGLGFVLSFGYWCTNFLIVQRAM
                     SAHSIAAARKTPLIGAIPKMFIPFLIIIPGIIALVLMNTPGKGFELPLNDAGQPIYDY
                     TIIMLLKQYLPAGVLGLGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATGNKA
                     ADEKHYLGVGRLATIFGVAVSIAAAYLASLFGNIMDFLQTIFSMINAP"
     gene            1435..2571
                     /locus_tag="PKM34_10655"
     CDS             1435..2571
                     /locus_tag="PKM34_10655"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNQ84094.1"
                     /translation="MTAINRNYLLIILLPAILYLNGCRHSHTPKNRKMITISKTPFGQ
                     VDGKPVDLYTLTSENMVVSITNFGGIVTSVKVPDRNGRMADVVLGFDNIDGYAGTHPY
                     FGCIVGRYGNRIARAGFSIDGTYHRLNANNGKNHLHGGISGFDKKIWNAAEIYREDEA
                     GIVLTCVSHDGDEGFPGELKVKVTYTITLNNELKIEYYAETDKPTPVNLTHHSYFNLK
                     GAGEGDILGHKLMIDADRYTVVDDELIPTGELRPVANTPLDFRNPAEIGRGIDSVPGG
                     YDHNFVLNGDGKLKKVARLSEESTGRLMEIYTDQPGLQFYSGNFLDGTIQGKQGMIYR
                     KHYGLCLETQHFPDSPNRPEFPSTILRPGETYTSTTIYKFSIIP"
BASE COUNT          724 a          642 c          637 g          608 t
ORIGIN      
        1 cggcgcattg atcatcgaga aaatggtctg taggaaatcc atgatgtttc cgaagaggct
       61 ggccaggtag gctgcagcga tactgacggc cacaccgaaa atggtggcca ggcggcccac
      121 cccgagatag tgtttctcat cggctgcctt attccccgtt gccggacgta tataactctg
      181 gtaaatatca taagtccaaa cggtgttgaa tgcggaaaca ttcccggcca tccccgacat
      241 aaaggaggcc agcagcgcgg taatacctaa tcccagcacc ccggccggca ggtattgctt
      301 caatagcata atgatcgtgt aatcgtatat gggttgtccg gcatcgttaa gcgggagctc
      361 aaatcctttt ccgggggtat tcatcaggac cagggcgatg atgccgggga taatgatcag
      421 gaaggggatg aacattttcg gaatggctcc gatcagggga gttttacggg ctgccgcgat
      481 ggagtgcgcc gacatggccc gctgcacgat cagaaaattg gtgcaccagt atccaaatga
      541 aaggacaaat cccagcccgg caaagatact gtaccactcc actcccatcg gatttgagcc
      601 gggcgaaccg gtatatttcc aggtagtggt ccatgtgtct ggggcaaagc ccttttcggt
      661 tgcaatcggg gccagttgtt cctgcaagcc ctgccatccg ccgacatctt tcaggctaag
      721 aaaaaccact ggcagcaagc cgatgacaat gatgaaaaac tgcaggacct cgttgtaaat
      781 cgcggatgac aaaccgccaa ggtaagtata gcccagcacg atggccgccg aaaggatcag
      841 gctgaggtga aaattccagc ccagcacctt ttccatcagg atcgccaggg cgtacatgga
      901 aataccggag gccaggatcg tcatcacggc aaacaggatc gcattcaatc cccttgtttt
      961 ctcatcgtat cttaatttca aatactccgg taccgatctg gcttttgaac cgtaataaaa
     1021 aggcatcatg aacagcgcca ggaataccat ggccgggatg gcacccaccc agtaaaaatg
     1081 tgtggtcagc atcccgtatt ttaatcctga accggtcatt cccattacct ccagtgcgcc
     1141 aaggttggtc gaaatgaaag ccaggcctgc tacccatgca ggcatcgacc gtcctgcttc
     1201 aagaaatgcg gtggcatcct tcatgtattt ctttaatgcc catccaatac cgagcacaaa
     1261 ggcaaagtag atgatcatga ccgcgtagtc gatccattcg agattaaatg tgttcgtcat
     1321 atcctttggt tttatcaatg taaaattaaa gatatatctg aattatgaca gcattatata
     1381 aaaagcgggg attttttttg acaattttac ctttacattt acatcttgtt aaatatgacc
     1441 gctattaacc gtaattatct gctgataatc ctgcttccgg ctatcctgta cctgaacggg
     1501 tgccgtcaca gccatacgcc caagaaccgc aaaatgatca ctattagcaa aacgcctttc
     1561 ggacaagtcg acggaaagcc cgtagatctc tataccctga catcggaaaa catggttgta
     1621 agtatcacca acttcggtgg gattgttact tccgtaaagg ttcccgaccg gaacgggaga
     1681 atggccgatg tagtcctcgg atttgacaac attgatggct atgccggtac ccatccctat
     1741 ttcggctgta tcgtcggcag gtatggtaac cggattgccc gtgccgggtt cagtatcgat
     1801 ggaacctacc acaggttaaa tgccaacaat ggtaaaaatc atcttcacgg aggcatatcg
     1861 ggatttgata aaaaaatctg gaacgccgca gagatttacc gcgaagatga agcggggatc
     1921 gtgctgacct gtgtcagtca tgatggcgat gaaggctttc ccggggaatt gaaggttaag
     1981 gtgacctata ccattacgtt gaacaatgaa ttgaagatag aatattatgc cgaaacggat
     2041 aaacctacac cggtcaatct tacccaccat agttatttta acctgaaagg cgcaggagaa
     2101 ggtgatatcc tgggccataa actgatgatc gatgccgacc ggtacacggt tgtcgatgat
     2161 gaactcattc ccaccggtga gctgaggcca gtggcgaaca caccactgga tttcagaaat
     2221 cctgccgaaa tcggaagggg cattgattcg gttccggggg gatacgatca taactttgtt
     2281 ttgaacggtg atgggaagtt gaaaaaagtt gcccggctaa gtgaagaatc gaccggaaga
     2341 cttatggaaa tctacaccga ccagcccgga ctgcagtttt acagcggcaa tttcctggac
     2401 ggaactatcc agggaaaaca agggatgatt taccggaaac actatggatt atgccttgag
     2461 acacagcatt ttcccgactc gcccaaccgg ccggagttcc cttctactat actccgcccc
     2521 ggcgaaactt acacatccac aacgatttac aaattttcca taatacccta aggcacaaaa
     2581 ccagcctggc tgggcaaaga ttggaaaata c
//
LOCUS       DAOQXS010000426         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_43961_length_2586_cov_4.260371, whole genome shotgun sequence.
ACCESSION   DAOQXS010000426 DAOQXS010000000
VERSION     DAOQXS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43961_length_2586_cov_4.260371"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKM34_10660"
     CDS             <1..1195
                     /locus_tag="PKM34_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84095.1"
                     /translation="AIPYGGTENYSYSWSSSPPGFTSDLPEVTVQPATTTTYSVSVSD
                     GLSEMTASVTITVHPNPQPDAGPDQLIPYGTNTTLSATASGGSGSYAYHWEPAALVSD
                     PDIPVTQTQNLIIATVFTLTVTDLETGCVSNPDPITISIDGGPLAVVITSDKPKICRG
                     EEAVLTAHASGGNQGNYIYSWSDNLGNSYPSLAQIKVSPEDTTVFHVSVDDGYNVNGY
                     DFTLVVFPSAEFGWADGSDHVFACPNDSVLLQPVPRDPSWNFLWSNGATTPEIFVTVT
                     GIGFSLQDYSLTTTTVDGCTFTDTILVSFDFSYCSGIEDEGETTVMISPNPASGAFKV
                     SWKKPGDFEAIRVYNGLGKMISQKETGHYNHVIFDHFKYSPGLYYVHLIGAKSSETRK
                     VLLMP"
     gene            1291..2250
                     /gene="menA"
                     /locus_tag="PKM34_10665"
     CDS             1291..2250
                     /gene="menA"
                     /locus_tag="PKM34_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNQ84096.1"
                     /translation="MEDQAKLSFFKKWWISARPFSFPASTMPVIFGTVLAYVYGGHEF
                     KPLLFILAFIAMVILHSGANILSDIFDFEKGLDKVPGPTSGGVVRGIISIREAWIGSV
                     SMLVFGTIIGLYLTWVSGPWLLAIGILGLLVGTFYTSHSMVNMKYNGLGDLAVFLNFG
                     ILGSLGAYYVQSGALSWIPVIWAIPMSMLVIAILHANNWRDIQSDKEIKIFTIASLLG
                     DKNSLRYYGVLIYGPFIIIVGLILVPFFLFPNMTAMPFTFLITLAALPLAFRVWKKAL
                     NRKTPAQPLDFIALDGASAQLNLLFGSLCTLALILQAVIDWAK"
     gene            2332..2550
                     /locus_tag="PKM34_10670"
     CDS             2332..2550
                     /locus_tag="PKM34_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84097.1"
                     /translation="MPSHKIINIRPAILISCLAVLMFSPMFVLRQVGPIDFWWWMTIN
                     LSILISLSIITDWEYYYILSKDFTKLYR"
BASE COUNT          593 a          682 c          596 g          715 t
ORIGIN      
        1 cgctataccc tacggaggta ctgaaaacta ttcctattcc tggtcatcct ccccacccgg
       61 ctttacatcc gatctcccgg aagtcaccgt tcaaccggct actacgacaa cctattccgt
      121 ttccgttagc gacgggctga gtgaaatgac tgcatcggtc acaattaccg ttcatccgaa
      181 tccccagccg gatgccggcc ctgaccagtt gatcccatac ggcaccaata ccacgctatc
      241 ggccactgca tcaggaggta gtggcagcta tgcataccac tgggaaccgg ccgctttggt
      301 ctcagacccg gatatacccg ttacgcagac ccagaacctg atcatcgcta ccgtttttac
      361 cctgacggtc accgaccttg aaacaggctg tgtcagcaat cccgatccca ttaccatcag
      421 catcgacggc ggcccgctgg cggtcgtcat caccagcgac aagccaaaga tctgccgggg
      481 cgaagaggcc gttctcaccg cacatgccag cggcggcaac cagggaaact atatctactc
      541 ctggagcgat aacctgggca attcatatcc ttcgctggcc cagatcaagg tatcgccgga
      601 agatacgacg gtctttcatg tttcggtcga cgatgggtac aatgtcaatg ggtatgattt
      661 caccctggtc gtctttccgt cggctgaatt cggatgggct gacggctctg accatgtgtt
      721 tgcctgtccg aatgattcgg tgttgctgca acctgttccc cgtgatcctt cctggaattt
      781 tctctggtcg aacggcgcga ctacccccga aatctttgtg accgtcacgg gaattggctt
      841 cagcctgcaa gattattcac taacgactac cacagtggat ggatgcacat ttaccgacac
      901 gatcctggtc tctttcgatt tttcatattg ctcaggtatt gaggatgaag gagaaacaac
      961 ggtgatgatc agcccgaacc ctgcctcggg ggctttcaaa gtttcctgga aaaaacccgg
     1021 tgattttgag gcgattagag tttataacgg tttgggtaag atgatttcac agaaggaaac
     1081 cggtcattac aatcatgtta ttttcgatca tttcaaatac tcccccggac tctattacgt
     1141 ccatttgatc ggtgccaaat catcagaaac ccggaaagtc ctcctaatgc cctgatccgg
     1201 aaattcttcc gaaaatcaaa tttcattatc tttgcagcct tgaacaaatc ttactcagta
     1261 ttgtttgagt atcaaccgga taataattta atggaagatc aggcaaaact ctcattcttt
     1321 aagaagtggt ggatatcggc caggccgttt tcattccccg cttccaccat gcccgttatt
     1381 tttgggacgg tgctggccta tgtttatggc ggacacgaat tcaagccgct gctgtttatt
     1441 ttagctttta tagcgatggt catccttcac tcgggggcaa atattctgag cgatatattt
     1501 gattttgaga aagggctcga taaggtcccc ggcccgacca gcggcggagt tgtcagggga
     1561 attatttcca tacgcgaagc atggatcggt tctgtctcca tgctggtttt cgggacgatc
     1621 atcgggctgt acctgacctg ggtgagcgga ccctggttgc tggctatcgg tattctcggc
     1681 cttttagtgg gtacgtttta cacctcccat tccatggtca acatgaaata caacgggctt
     1741 ggcgacctgg ccgtattcct caatttcggc atcctgggct cgctgggcgc ctattatgtg
     1801 caatccggcg ctctatcatg gattcctgtc atctgggcca tcccgatgtc gatgctggtc
     1861 attgccatcc tgcatgccaa taactggagg gatatccagt cggacaaaga gatcaagata
     1921 tttaccatcg cctcattact gggagataag aattcattac ggtattatgg cgttttgatt
     1981 tacggaccgt ttatcatcat agtgggcctg atcctggtcc cgtttttcct gttcccgaac
     2041 atgaccgcca tgccgtttac attcctgatc acgctggccg cgcttccgct ggctttcagg
     2101 gtctggaaaa aagcgctgaa ccggaaaaca ccggcccagc cgcttgattt cattgccctc
     2161 gacggcgcat cggcgcaatt gaacctgctt ttcggatcgc tctgtaccct ggccctgatc
     2221 ctccaggctg ttattgactg ggcaaaatga gatggcatct caaatcaacg tcgtattgtt
     2281 tataactaac tataaataag tttattggct tcaacaaaat agggattcgg gatgccatct
     2341 cataagatca ttaatatccg acctgccatt ctcatctcct gcctggccgt cctgatgttc
     2401 agcccgatgt ttgtgttgcg ccaggtcggt cccatcgact tctggtggtg gatgacgatc
     2461 aacctgtcaa tcctcattag tttgtcgatt atcaccgact gggaatatta ttacatctta
     2521 tcgaaagatt tcacgaagtt ataccgttaa tttcctaata taaattcaaa attattttat
     2581 ttttga
//
LOCUS       DAOQXS010000427         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_44144_length_2580_cov_2.513663, whole genome shotgun sequence.
ACCESSION   DAOQXS010000427 DAOQXS010000000
VERSION     DAOQXS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44144_length_2580_cov_2.513663"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PKM34_10675"
     CDS             complement(<1..1461)
                     /locus_tag="PKM34_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNQ84098.1"
                     /translation="MPENKSVRTIQSLTEKAWWKEAVVYQVYPRSFKDSDGDGVGDLK
                     GIIEKLDYIKSLGVDVVWLNPIFASPNDDNGYDISDYRAIMKEFGTMEDFDNLLQGMH
                     DRGIKLVMDLVVNHCSSEHPWFVEARSSRENTFRDYFHWWPAEKGKPAKRYSFFDVNN
                     DAWKYDSLTDSYYLHYFSVKQPDLNWENPKVRTEIYDMMKFWFDKGIDGFRMDVIPFI
                     SKDTTFPELPAEYNGDMVRYYANGPHLHEYLQEMNREVLSKYDIMTVAEGAGITYREA
                     LDFVDPERKELNMAYHFDGMNIGYLPNQFKVMNPEGYDLLEFKRVYSLWDSVFAEKGW
                     GTIYLGNHDQPRMVTRWGNDSPEFRELSSKMLTTFLLTMRGTPYYYFGDELGMKNIRF
                     DHIEDYRDIESVNMYQQVKERGGDLQAFLEAQKISARDNGRTPFQWDDSPNAGFTSGT
                     PWLKVNPDYITVNAAAQENDPNSVLNYFRKLIILRKN"
     gene            complement(1710..2219)
                     /locus_tag="PKM34_10680"
     CDS             complement(1710..2219)
                     /locus_tag="PKM34_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84099.1"
                     /translation="MNSPIGKITFWGSDLTYTYNYVGCIPSEGKTFTFAFYENAEGAV
                     GNLVTSFQAIPAKAYCLPPFAYQDAPIFKYEVELPAPITMTDGWLSILGTGGTDGCWF
                     GWCIASSGDNFMYQACYSSFSYYELDLAFTLGPPPENPGVVIPISNWALMLALGLILT
                     FAVFRFRKL"
     gene            complement(2244..2453)
                     /locus_tag="PKM34_10685"
     CDS             complement(2244..2453)
                     /locus_tag="PKM34_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84100.1"
                     /translation="MKNFSLILLLLLAITVSGQVVPNEGLLIENSSANLRTAIEPCTL
                     CDEGSVFCNTPSPYAGVGLSDDQRC"
BASE COUNT          666 a          620 c          611 g          683 t
ORIGIN      
        1 gtttttgcgg aggatgatca gtttcctgaa ataattcagg actgaattgg gatcattttc
       61 ctgggctgcg gcattgactg taatataatc cgggttgact ttcagccagg gcgttccact
      121 ggtgaagcct gcattgggcg aatcatccca ctggaaggga gtgcggccgt tatcgcgggc
      181 tgagatcttc tgcgcttcaa gaaatgcctg caggtctcct cccctctcct tcacctgttg
      241 gtacatgttg accgactcaa tatcccggta atcctcaatg tgatcgaacc tgatattctt
      301 catgcccagt tcatcgccaa aataataata tggcgtgccg cgcatggtca gcaggaaggt
      361 cgtgagcatt ttggaagaaa gttcgcggaa ttccgggctg tcgtttcccc agcgggtgac
      421 catgcggggc tggtcgtggt tccccagata gattgtgccc cagccttttt cggcgaagac
      481 actgtcccag agcgaataaa cgcgcttgaa ttccagcagg tcgtaacctt ccgggttcat
      541 caccttgaac tggttgggca ggtaaccgat gttcatcccg tcgaaatgat aggccatgtt
      601 cagttctttc cgctcaggat ccacgaaatc gagggcttcg cgataggtga tcccggcgcc
      661 ttcggcaacg gtcatgatat cgtattttga aagcacttcc ctgttcatct cctgcaggta
      721 ttcgtgcagg tgcggcccgt tggcatagta tctcaccatg tcgccgttgt attcggccgg
      781 cagctccggg aaagtcgtat ccttgctgat gaaagggatc acatccatcc ggaagccgtc
      841 gatcccttta tcgaaccaga atttcatcat gtcgtagatc tccgtcctga ccttcgggtt
      901 ttcccagttc aggtcgggtt gtttgaccga gaaatagtgc aggtagtacg aatcggtcag
      961 gctgtcgtat ttccacgcat cgttgttaac gtcgaagaag ctgtaccgtt ttgccggctt
     1021 acccttctcg gccggccacc agtggaaata gtcgcggaaa gtgttttcgc ggctgctgcg
     1081 ggcctccaca aaccaggggt gttcggagct gcagtggttg accaccaggt ccatcaccag
     1141 tttgatcccc cggtcatgca tcccttgcag aaggttatca aagtcctcca tcgtgccgaa
     1201 ctctttcatg atcgcccggt aatcactgat gtcgtacccg ttgtcgtcgt tcggcgaggc
     1261 gaagatggga ttaagccaga ctacatcgac accgaggctt ttaatgtaat cgagcttttc
     1321 gatgatccct ttcaggtcgc cgacgccatc gccgtctgaa tctttaaagc tgcgggggta
     1381 gacctggtag acgaccgctt ctttccacca ggctttttca gtaagtgatt gaatggttct
     1441 aactgattta ttttcaggca tctgacagga ggagattaat atagatatca gaaagatgat
     1501 tttacaaaac gataatttga ttttcatgat aaattgataa agccaatatt ggaaccggcg
     1561 catcgggatg caaagatata tccggaaatc aaatgaaagg aagaatcgtg caaatccgtt
     1621 taatccgtct cattcgtgtt ccattaattg aacacgaatc acacggataa aacgaattat
     1681 cgcggatgta ttatggaaat gctgaccgtt tacagttttc tgaagcggaa aacggcaaaa
     1741 gtcaggatca agccaagtgc cagcatcaac gcccagtttg agatggggat gaccacgccc
     1801 gggttctccg gtggcggacc cagcgtaaat gccagatcaa gctcataata ggagaaagat
     1861 gagtaacacg cctggtacat aaaattatca ccggaagagg caatgcacca tccaaaccag
     1921 caaccatccg taccacccgt tcccagtatc gacagccatc catcggtcat ggtgattgga
     1981 gcaggcaatt ctacttcata tttaaaaatt ggagcatcct ggtatgcaaa agggggcagg
     2041 caataagcct ttgcgggaat agcctggaaa gaggtaacca ggtttcctac cgcgccttcc
     2101 gcattctcat aaaaagcaaa ggtaaaagtt ttgccttcag aaggtatgca acccacataa
     2161 ttataagtat aggtcagatc acttccccag aatgtaattt ttccaatagg tgaattcaca
     2221 ttgtaaaaat tatcgtagca cactcaacac cgctggtcat cacttaatcc aaccccagcg
     2281 taaggacttg gcgtattgca gaacacagaa ccttcgtcac acaatgtgca tggttcgata
     2341 gcggttctta aatttgcact gctgttttca attagtaatc cctcattggg aacaacctga
     2401 ccggaaacag tgatcgccag taataacaat aaaatcaggc taaaattttt catggtatgt
     2461 agtttttggt ttgattggaa gtaagattgg ttaaaaatac aataaatttc aaaacaccat
     2521 attatttttt cttaaagaag atatatccgc ctttctccga tccccagacc tgcttgtctt
//
LOCUS       DAOQXS010000428         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_44884_length_2552_cov_4.048859, whole genome shotgun sequence.
ACCESSION   DAOQXS010000428 DAOQXS010000000
VERSION     DAOQXS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44884_length_2552_cov_4.048859"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..536)
                     /locus_tag="PKM34_10690"
     CDS             complement(30..536)
                     /locus_tag="PKM34_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ84101.1"
                     /translation="MTVIEFNYKLLSMQSYLQYFAKKLTNNEEDANDLVQETNYKALL
                     FREKYVHHTNFKAWLFTIMKNVFINNYRRTKKARTFIDNTDNLHYIHQHIDVHPVTPD
                     SELREKEVRKAVSQLEDEQRIPFEMHTQGFKYKEIAEELDISIGTVKSRIFFGRKKLM
                     DKLEGYGN"
     gene            complement(747..1631)
                     /locus_tag="PKM34_10695"
     CDS             complement(747..1631)
                     /locus_tag="PKM34_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ84102.1"
                     /translation="MANYSIKDLERISGVKAHTIRIWEKRYGIVEPKRTDSNIRYYCD
                     HDVKKLMNVAILLNHGYKISKLAGLDCSEISKKVLEVSMVANGYDSQIENLVIAMIEL
                     DESRFEKLLNQSIIKDGFENTVFKVLYPFFERIGVLWQAGSINPAHEHFITNLVKQKI
                     YVAIDSIPVPAGAEVRKFLLFLPEWDLHELGLLVYDYILKSRGFKVIYLGSNVPEEDV
                     YSVADYLKPDYFLTAFANAIEPEKLESYINRVAGRFPGSKIFITGFQAQAMPPAFPHN
                     VVRVNSAQQFSSEILPGL"
     gene            1757..>2552
                     /locus_tag="PKM34_10700"
     CDS             1757..>2552
                     /locus_tag="PKM34_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNQ84103.1"
                     /translation="MKRLDKEIQDIVVIGSGNVAYHLICAFTGIGISVSQIYSRNEKT
                     AALLSGKFGIPYIVDPNLLNRNAGLYILAVQDSNIREAALSLNLEDHLLVHTSGFSPL
                     GILEGTAAFTGVIWPVQTLTSGKDTDYRSIPIFTEGSCNETRIMLNNLAGRISHKVVL
                     TDTPTRQRVHLAAVISSNLVNHLYSVSASILESVNLPFDILRPLIHETAIKATAALPS
                     DAQTGPAARHDMEVIRQHLEMLRDNPLQRDIYQLITNSILTSGPDKH"
BASE COUNT          691 a          593 c          527 g          741 t
ORIGIN      
        1 agccaaaaac gttccctttt cccttttccc taattcccgt acccctcgag cttgtccatc
       61 agcttctttc tgccaaagaa tatcctgctt ttcactgtgc cgatcgatat atcgagttct
      121 tcggcaatct ccttgtattt aaaaccctgc gtgtgcattt cgaacggaat cctttgctcg
      181 tcttcgagct ggctgacggc ttttcttacc tctttctcgc gaagttcgga atcgggtgtt
      241 acaggatgca catcaatgtg ctgatgtata tagtgcaggt tatccgtgtt atcgataaaa
      301 gtccgcgctt ttttcgtccg cctgtagttg ttgatgaaca cattcttcat aatagtgaac
      361 aaccaggctt tgaaattggt atgatgcaca tatttttcac gaaacaataa ggctttataa
      421 ttcgtttcct gaaccaggtc gttagcatct tcctcattgt tggtcagctt tttagcaaag
      481 tactgaagat aactctgcat gctgagaagc ttataattga attctattac tgtcatggct
      541 cgtatatttg tttaattatt atgcgacaaa gttaaacata aatacaatca cttgtcaagt
      601 tttttaacat ttttttaaga attattctaa ataagaatat tgttagcatt gttattcaat
      661 gctttaaagt atttaaagta atcagaattt aaatagaatt gttaaacgat gttaaaatat
      721 cagccgataa ggtttaacag aaacggttat aaaccgggga ggatttcgga agaaaactgc
      781 tgagccgaat ttacccggac tacattgtga gggaatgccg gcggcatagc ctgagcctga
      841 aaaccggtaa taaagatttt tgaaccggga aagcggccgg caaccctgtt gatatatgat
      901 tccagttttt caggttcaat tgcattggca aatgcagtca gaaaataatc gggcttcaga
      961 taatcagcca ctgaataaac atcctcctcc ggcacattcg agcccaggta gattacttta
     1021 aaaccccggc ttttaaggat atagtcgtaa accagaagac ctaactcatg cagatcccat
     1081 tcgggaagga acagtaaaaa tttcctgact tcggctccgg caggcacggg aatactgtcg
     1141 atggcgacat aaattttctg cttaacgagg ttagtaatga agtgctcgtg cgctggattg
     1201 atgctcccgg cctgccacag cacgccaatt ctttcaaaga aagggtaaag caccttgaac
     1261 actgtatttt caaatccgtc tttaatgata gactgattca gcagtttctc gaacctggat
     1321 tcatccagtt cgatcatggc gatgaccagg ttctcaatct ggctgtcgta cccattggca
     1381 accatcgaaa cttccagtac ctttttggaa atctcgctgc aatcgagacc ggcaagtttg
     1441 gagatcttat agccatgatt cagcaggata gcgacattca tcagcttttt cacatcatgg
     1501 tcacaataat agcggatatt ggagtcggtc ctctttggct ccacgatgcc ataccgcttt
     1561 tcccagatac ggatggtatg tgcttttacg ccgcttattc tctcaagatc tttaatgcta
     1621 tagttcgcca tggaatgatg aatacgtcgg gataaacaat acgaatatga ttttgttcac
     1681 ttttaatgca aagcaaatta cgcacaattt ctgaaatcaa accatccggt caaatatatc
     1741 ggttaatttt gcacgaatga aaaggttgga caaagagatt caggatattg tcgttatcgg
     1801 atcgggaaat gttgcctatc atttaatatg cgcttttacc ggcattggca tttccgtctc
     1861 ccaaatctat tcccgaaacg aaaaaactgc cgctcttctg tccggcaaat ttggtatacc
     1921 ttatatagtt gaccccaacc ttctcaaccg aaatgccggc ttgtacatac tggcggtcca
     1981 ggacagcaac atccgggaag cggcgttaag cctcaacctg gaggatcatc tgttagttca
     2041 cacatcgggt ttctccccgc tcggcatcct ggaaggcaca gcggctttta ccggcgtgat
     2101 ctggccggtt cagaccctca cttccggaaa agataccgat taccgttcca tcccgatttt
     2161 tacagagggt tcctgcaatg aaaccaggat catgctgaat aacctggccg gcaggatcag
     2221 ccataaagtt gttttaaccg acactccgac gcggcaaagg gttcacttgg ccgcggtcat
     2281 ttcatccaac ctggtcaatc atctgtattc agtttcagca tccattctcg aatcggttaa
     2341 ccttccgttc gatatcctgc gtccgttgat tcatgaaact gcaataaagg ccaccgctgc
     2401 cttgccctcc gatgcacaga caggtccggc ggcccggcac gatatggagg tgatccggca
     2461 acatctcgaa atgctgcggg ataatcccct tcagcgggat atatatcaat tgataaccaa
     2521 cagtatttta acctcaggcc ctgataagca tg
//
LOCUS       DAOQXS010000429         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_45348_length_2536_cov_3.241838, whole genome shotgun sequence.
ACCESSION   DAOQXS010000429 DAOQXS010000000
VERSION     DAOQXS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45348_length_2536_cov_3.241838"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..595)
                     /locus_tag="PKM34_10705"
     CDS             complement(197..595)
                     /locus_tag="PKM34_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNQ84104.1"
                     /translation="MSEIAGCGIDIEELARFSRHLPVNESIPDLWHMISTEEEISINR
                     QVSPHVSFPLSFSCKEAMFKALGRSWTNSNISWKEIELIFQDHQLGGDFLIRLSGHAL
                     ERYRALNCTRIESAFSFTDDYVIFQIILHS"
     gene            complement(606..911)
                     /locus_tag="PKM34_10710"
     CDS             complement(606..911)
                     /locus_tag="PKM34_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNQ84105.1"
                     /translation="MIEITDEIRSDVREIVCQFFAEQCEVEIGSINDQTNIITDLEGD
                     SLMFLELIEIFKKKYNLDIELKTVGKYVLKHPVETIGQLIETQLLMIQYGNQILEMK"
     gene            complement(948..2162)
                     /locus_tag="PKM34_10715"
     CDS             complement(948..2162)
                     /locus_tag="PKM34_10715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNQ84106.1"
                     /translation="MPKKVVITGMSLITSLGLDLQTNWDNLIAGRNGVKHISLFDPTD
                     LQTQVAAQVSEDFEACSTKYLKKRTADQMTRVTRMAFVCATDAIKDAGVDFEAQDRTR
                     CGVILGVVSTGNTSAEKGTTARNRVLKSMNNAMPAWISLEHKLMGPNFTVSSACSSSA
                     YAIGLGYDLIRNGAADIVITGGADSIINREEIEGFNELYALSVNNDPETACRPFSLDR
                     DGFVIGEGAGILVLESEESAKKRDARIYAELAGYAITSEAYNIMAPMKDGEGICHTIH
                     EALKNAGVKPEEVEYINAHGTSTILNDRYETMAIKKVFGEGAYRIPVSSTKSMIGHTV
                     GAAGAIEGIVTILSIYHDVITPTIHLDQPDPELDLDYVPHTSRNQLVNCALSNSFAFG
                     GHNATLVFKKYA"
     gene            2293..>2536
                     /locus_tag="PKM34_10720"
     CDS             2293..>2536
                     /locus_tag="PKM34_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84107.1"
                     /translation="MWNNSALISAPTTKAVGSEQVKKMKRLSQKSIYSLFKGSPTTEA
                     VGSNPNKTFGTNGFSRWSTNCLQVIKSKCQIFETPSL"
BASE COUNT          620 a          611 c          604 g          701 t
ORIGIN      
        1 tgtgatttca gctcgtcatt cagcatcggg ttttcagcaa aatcaaacct ggtaagatct
       61 gtatccaggg tatcggaggc cagggcgacc aactggcgga aatgggcttc ccggccccgg
      121 agcgcctggc cctctttgcc ggtatactca cccggaaatg caggaaaaat gatacaggtt
      181 ccgataacgc cagattttaa gagtgtaaaa ttatctgaaa aattacatag tcatccgtga
      241 aggagaaagc ggattcgatt ctcgtgcagt ttaaagcccg gtaacgttcc agggcatgac
      301 cggacagccg gatgagaaaa tctcccccga gttgatgatc ctgaaagatc agttcgatct
      361 ctttccagga aatattgctg ttggtccagc tccttccgag ggctttgaac atggcttcct
      421 tacaggaaaa tgagagggga aatgaaacgt ggggcgaaac ctgccggttg atactgatct
      481 cctcttcggt agagatcatg tgccatagat caggaatgga ttcgttaacg ggtaaatgcc
      541 tggagaaccg tgccagctct tcaatatcga tcccacatcc ggcaatttca ctcatcgggt
      601 tgcaactatt tcatttccaa aatctggttc ccgtattgta tcatcagcaa ctgggtttcg
      661 atcaattgtc cgatggtttc caccgggtgt ttcaggacat atttcccgac ggttttcagt
      721 tcgatatcga gattgtattt ctttttaaag atttcgatca attccaggaa catgagcgaa
      781 tcgccttcga gatcggtgat gatgttggtc tggtcgttaa tggagccgat ctccacttca
      841 cactgttcgg caaagaactg gcagactatt tctcgtacat cacttctgat ttcatctgtt
      901 atttcgatca tatacaggtt atgttttttg ggattaagag ggtaaaatta ggcatatttc
      961 ttaaatacca gcgtggcatt atgtcccccg aaggcaaatg aattggataa ggcacagttt
     1021 accaactggt tgcgcgaagt gtgcgggaca tagtcgaggt ccagttcagg atccggctga
     1081 tcgagatgga tcgttggcgt gatcacgtca tgataaatgc tcagaatggt cacgatccct
     1141 tcaatggcgc cggcagctcc cacagtatgc ccgatcatcg actttgtcga tgaaacggga
     1201 atccggtaag ccccttctcc gaaaaccttt ttaatggcca tggtttcgta gcggtcgtta
     1261 agaatcgtcg acgtcccgtg ggcattgata tactccactt cctccggttt tacgccggca
     1321 ttttttaacg cctcatggat ggtgtggcag ataccttccc cgtctttcat gggggccatg
     1381 atgttgtaag cttcgctggt tatggcatac ccggccagct cggcgtaaat ccgggcatct
     1441 cgttttttag ccgactcttc cgattcgaga acaaggatac cggcgccttc cccgatgaca
     1501 aacccgtcgc ggtcaagcga aaagggacgg caggcggttt cggggtcatt gtttaccgac
     1561 agggcataca gttcgttgaa tccctcgatc tcttcgcggt tgatgataga gtcggccccc
     1621 ccggtgatca cgatatcggc cgcgccgttg cggatcaggt cgtatccgag gccaatggca
     1681 tatgccgacg aagagcaggc agaggatacg gtaaaattag gccccatcag tttgtgctcc
     1741 agagagatcc aggccggcat ggcattgttc atgcttttta gcacccggtt ccgtgccgtc
     1801 gtaccttttt cggcggatgt gttaccggta cttaccacac cgagaatgac accgcacctc
     1861 gtcctgtcct gagcctcaaa atccactccg gcatctttta ttgcgtcggt tgcgcataca
     1921 aatgccatcc gggtgaccct tgtcatctgg tcggcggtcc gcttcttcag gtattttgtc
     1981 gagcaagcct caaaatcttc ggaaacctgc gctgcaacct gcgtttgcaa atccgtggga
     2041 tcaaacagcg aaatgtgttt aacgccgttc ctgcccgcta tcagattatc ccagtttgtc
     2101 tgaagatcca atcccagcga agtaatcagg ctcattcccg taatgaccac ttttttcggc
     2161 atttttccgg ttttatgatt gagtaaatga ttccgttttc aacttctggc aaaataaata
     2221 aaactttcct tctatttttg tacggggaaa ttctttaaaa ttatttttga gctgaaaatg
     2281 aaattattca ctatgtggaa taattcagct ttaattagtg ctccaactac taaagccgtt
     2341 ggttccgaac aggttaaaaa aatgaagagg ctgtctcaaa agtccatata ttcacttttt
     2401 aaaggtagtc cgacgactga agccgtcggt tccaacccta ataaaacatt cggaaccaac
     2461 ggctttagtc gttggagcac gaactgtctt caggtaatca aatcgaaatg tcagattttt
     2521 gagacaccct cactac
//
LOCUS       DAOQXS010000430         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_45437_length_2533_cov_2.710654, whole genome shotgun sequence.
ACCESSION   DAOQXS010000430 DAOQXS010000000
VERSION     DAOQXS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45437_length_2533_cov_2.710654"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PKM34_10725"
     CDS             <1..1326
                     /locus_tag="PKM34_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNQ84108.1"
                     /translation="SYKLIIIAFFAIMTLESFSQRSTIELTFTAVDDNAYVQLDSIRI
                     MNLTQGGDTLLFWPDTVLVLYYTGLGESLDRNGTFRVFPGYPNPAADQAIVSLFVPEK
                     DEVTITVTDLPGRVMISSERILEKGKHAFRFTPGGEGLFFFTARWRGRGSTIKILKTG
                     FGQLRLSSLDYLGSEGFSPRLKAGSADQGFSFTPGDELLYIGYHNGLQSGILDFPQES
                     TGYTFQFAANIPCPGTATVEYEGQVYNTIQIFSQCWLKENLNAGVRINGTAEQLNNGI
                     IEKYCYNDSPDSCIKYGGLYQWNEMMQYTTGQPVRGICPPGWHIPTDGEWKILAGAVD
                     SQYGIGNEEWNGADWLGYDAGSNLKAADGWSGNGNGTDLFGYSGLPGGGYYLLIEFYY
                     VGYYGSWWSSVENNEYDSWGYTFGYDKTEISRYNFSRSSGFSVRCLKDN"
     gene            1412..>2533
                     /locus_tag="PKM34_10730"
     CDS             1412..>2533
                     /locus_tag="PKM34_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Por secretion system protein"
                     /protein_id="HNQ84109.1"
                     /translation="MKKIIPALLFGLFSFSLFAQLISTEPPLPVITQPVVVTFNAAEC
                     SGGLAGYDGDVYAHTGLITETSGSGSDWKYVKTNWGVNTPDTKLTRIGQDLYELEISP
                     SIREYYGVPASETVLKMAFVFRSGVQVEGAWLEGKTEAGGDIFVDVYEAGLSVSFSKP
                     LYFPVIVQINESFTVEVNANEADNVSLYIDETLVKSMAGNYLSDTITASAYGKYLVKA
                     VATNDTGVVADSFYYHVRPAVTVETLPAGIRDGINYTGDQTAILSLFAPYKNFIYVIG
                     DFNNWDIDADFFMKKTPDGKRFWLEIDGLTPGQEYVFQYYIDGQIRVGDPYADKISDP
                     WNDEYISEETYPGLIPYPEGKTTGIATVLQTAQTPYAWEV"
BASE COUNT          618 a          608 c          678 g          629 t
ORIGIN      
        1 tcttataaat tgatcattat tgcttttttt gcaatcatga ctttggaatc gttttcccag
       61 cgttcaacga tagagctgac tttcacggct gtcgacgaca acgcctatgt tcagcttgac
      121 agcatcagga tcatgaacct tactcagggc ggagatactc tgctgttctg gcccgatacg
      181 gtccttgtgc tttattatac aggcctgggt gaaagcctgg accgtaacgg tacattccgg
      241 gttttccccg gctatcctaa tcctgcggcg gatcaggcca ttgtatcgtt atttgtgccg
      301 gaaaaagatg aggtcaccat caccgttacg gatctcccgg gtcgtgtaat gataagtagt
      361 gagcggatat tggaaaaagg gaagcatgct ttccgtttca cgccgggcgg ggagggcctg
      421 ttctttttca cggccaggtg gagggggcga ggcagcacca tcaaaatcct gaagacggga
      481 tttggtcaat tacgattgag ttcccttgat tatcttggca gtgaaggttt ttcgccccgg
      541 ttgaaagccg gttcagccga ccagggcttt tcttttaccc ccggtgatga gttgttgtat
      601 attggttacc ataacggtct ccagtcaggc attctggact tcccgcaaga gagcaccggc
      661 tatacttttc aatttgccgc taatatcccc tgcccgggaa cggccacggt ggaatacgaa
      721 ggtcaggtgt acaatactat ccagatattc agccagtgct ggttaaagga aaacctgaac
      781 gccggcgtca ggatcaacgg aacggctgaa caactgaata atggaattat tgaaaagtat
      841 tgttataacg atagtcccga cagttgcata aaatatggcg ggctttacca gtggaatgag
      901 atgatgcagt acacgaccgg gcagcctgtc cggggcattt gcccgccggg ctggcacatt
      961 ccgacggacg gggaatggaa aatcctggca ggagccgtcg acagtcagta tggaattgga
     1021 aatgaggaat ggaatggagc agactggctg ggatatgatg cgggttcaaa cctgaaggct
     1081 gccgatggct ggtcgggtaa cggcaacggg acggatcttt tcggttattc cggtctgccc
     1141 ggaggcggtt actatcttct tattgaattt tactatgtag gatattatgg gagctggtgg
     1201 agttcagtag agaacaatga atacgactca tggggttaca cctttggcta cgataaaaca
     1261 gaaatcagcc ggtataattt cagcaggagc agcggtttca gtgttcgttg cttgaaagac
     1321 aattgattat ttcctccgac gtattacgaa tagacagggc atcttccacc ccggaaattc
     1381 attacctttg cctggattat caatccctgc tatgaaaaaa atcatccctg ccctgctatt
     1441 tggcttattc agtttttccc tcttcgccca gttgatctca acagaaccgc cattgccggt
     1501 tatcacccag ccggtggtcg tcacttttaa tgcggcagag tgctcgggag gcctggccgg
     1561 ctacgacggc gatgtctatg cccatacggg cctgatcacc gaaaccagcg gcagcggctc
     1621 cgactggaaa tatgtcaaaa cgaactgggg agtcaatacg ccggatacaa agctgacacg
     1681 gatcggccag gatctctacg agctggagat ttcaccgtct atcagggaat attacggtgt
     1741 tccggccagc gaaacggtgc tgaaaatggc ttttgtcttc cgcagcggtg tccaggtcga
     1801 aggcgcctgg ctcgaaggga aaaccgaagc gggtggagat atctttgttg atgtctacga
     1861 agccggcctt tcggtctcct ttagcaagcc gctctatttc ccggtcatcg tgcagatcaa
     1921 tgagtcgttt accgtggagg tcaatgccaa tgaggctgac aatgtttcac tttatatcga
     1981 tgaaaccctg gtaaaatcga tggcgggaaa ttatctatcg gataccatta ccgcttcggc
     2041 atacggaaaa tacctggtca aagcggtggc cacgaacgat acgggcgttg tagccgattc
     2101 gttttattat catgtcaggc cggctgttac cgtcgaaacc ctgcccgccg gtatccgcga
     2161 cgggatcaat tatacaggcg accagacggc cattctcagt ctctttgcac cctacaagaa
     2221 ttttatctac gtaatcggcg atttcaacaa ctgggatatt gatgccgact tttttatgaa
     2281 gaaaacaccc gacggaaaac ggttctggct tgagatcgac gggctgacgc cgggacagga
     2341 atatgtgttc cagtattaca tcgatggcca gatcagggtc ggcgatccct atgccgataa
     2401 gatcagcgat ccctggaacg atgaatacat cagcgaggag acctacccgg ggctgatccc
     2461 atacccggaa gggaaaacaa ccggcatagc caccgtcctg caaaccgccc agacgcctta
     2521 tgcctgggag gtg
//
LOCUS       DAOQXS010000431         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_45628_length_2526_cov_3.698503, whole genome shotgun sequence.
ACCESSION   DAOQXS010000431 DAOQXS010000000
VERSION     DAOQXS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45628_length_2526_cov_3.698503"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKM34_10735"
     CDS             complement(<1..330)
                     /locus_tag="PKM34_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84110.1"
                     /translation="MKIRLSYTLILLVISLFTNAQVAEYYFTQQNGTYTEINGGTELW
                     ATSFDNEVSGAVTIPSFTYDGTAYTAIYISVNGFITFGSAPAGTVYNPIGNTATYSGA
                     ISAFGRDL"
     gene            complement(470..790)
                     /locus_tag="PKM34_10740"
     CDS             complement(470..790)
                     /locus_tag="PKM34_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84111.1"
                     /translation="MDDNEQLAGLINKDLGDPDEGLLIPVSGIDRSEIHQKLSGMIAY
                     LIERHFERLCQAMYRLDVSERKFHQVMNEKPLDEIPGALADLVIEREIQKIHTRRMYK
                     SGQL"
     gene            complement(783..968)
                     /locus_tag="PKM34_10745"
     CDS             complement(783..968)
                     /locus_tag="PKM34_10745"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNQ84112.1"
                     /translation="MPVPSPCKLICRYDSDGLCAGCRRTREEITRWISYSDHEKLEVY
                     KKITLRQQSVSNAQHHG"
     gene            complement(1046..1918)
                     /locus_tag="PKM34_10750"
     CDS             complement(1046..1918)
                     /locus_tag="PKM34_10750"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNQ84113.1"
                     /translation="MKIYLIPVLLLIFSTVSSGQDLMDMLGDDEANTTDYTYATFKAT
                     RIINGQSIELPPKGALNFVISHHFGRINQGAYEFFGLDQANIRFGFEYGFTDWLSAGI
                     GRTSVNKTYDGSLKAKILRQSSGKRSMPITLTWFSNATISSLKWQEPDRKNYFTSRME
                     FAHQLLIARKFGSRVSLQLMPTYVHRNLVETIEDENDVFAVGAGGRIKITNRMAVTGE
                     YYYVLPGKTADDFHNSFSIGLDLETGGHVFQVYLTNSQGLIEEQFIPRTSGNWGDGDI
                     HIGFNINRTFQIVK"
     gene            complement(1955..2398)
                     /locus_tag="PKM34_10755"
     CDS             complement(1955..2398)
                     /locus_tag="PKM34_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84114.1"
                     /translation="MKKVLLIIIPVVVLLGAGGYFGYSYFFAEKPVAEVVLGPAEVKV
                     NAKRLWVDYSMNPDIAGPRYTGKIAEVTGSIMRVEAVDDEIVVVFAFKKGDAGDEGIR
                     VTMLPSFRQAARGINPFKNVTIKGVIKEYDRQNIIMEHGSIIGKQ"
BASE COUNT          591 a          637 c          621 g          677 t
ORIGIN      
        1 cagatcgcgg ccaaatgccg aaatagctcc ggaataggtt gcggtattgc caatgggatt
       61 ataaactgtc cccgccgggg cgctcccgaa agtaataaac ccattaacac tgatataaat
      121 ggctgtgtag gccgtaccgt cgtatgtaaa cgaagggatg gtcaccgccc cggatacttc
      181 gttgtcaaaa gatgtagccc ataattccgt cccaccgttg atttcggtat aggtaccgtt
      241 ctgttgtgta aaatagtact ccgctacctg cgcattggta aacaggctga taactaataa
      301 gataagggta taacttaacc ggatcttcat ggcgcttgct gttttatttt ggttggatta
      361 ggacaaagca ataagatctc gtttttgagt tcttaaaaat aatgaaaatt caatgaaaat
      421 aaaaagaaat atcttgaatt ttcagtaata acagggaacg cgataaagtc tacaattggc
      481 cgcttttata catccggcgg gtatggatct tttgaatttc gcgttcgatg accaggtcgg
      541 ccagggcgcc gggaatctca tcgagcggct tttcgttcat cacctggtgg aatttgcgct
      601 ccgacacatc gagcctgtac atggcctggc agagccgctc aaagtggcgc tcgatcaggt
      661 aagcgatcat cccggagagt ttctgatgaa tttcgctccg gtcgatgccg gagacaggga
      721 tcaacaggcc ttcgtcagga tcgccgaggt ctttgttgat caatccggcc agttgttcgt
      781 tatcatccat gatgctgcgc atttgataca ctttgttggc gcaaagttat ctttttatag
      841 acttccagtt tttcgtggtc ggaatagctg atccagcggg tgatctcttc tctggtccgc
      901 ctgcacccgg cacaaaggcc gtcggagtcg taccggcata tcagtttaca aggacttgga
      961 acgggcattt tttttgttgc taaacgattg catacataaa attgttcata tgccggtaaa
     1021 tccttctatt agtagtccga cggctttatt ttacaatctg gaatgtccgg ttgatattga
     1081 acccgatatg aatgtcgcca tcgccccagt tgccgctggt tctgggaatg aactgctctt
     1141 cgatcagccc ctgcgagttg gtcaggtaaa cctggaagac atgcccgccg gtttccaggt
     1201 ccaggccgat ggagaatgaa ttgtggaaat catcggctgt tttgccgggc agcacgtagt
     1261 aatattctcc cgttacggcc atccggttgg tgatcttgat cctgccgccg gcgccaactg
     1321 cgaaaacgtc gttctcgtct tctatcgtct ccaccaggtt ccggtggaca tacgtcggca
     1381 tcagctggag cgatacacgc gacccgaact tgcgggcgat cagcagttga tgggcaaact
     1441 ccatgcggga agtgaaataa ttcttccggt cgggctcctg ccatttcagg gaagaaatgg
     1501 tggcattgga gaaccaggtc agggtaatgg gcatgctccg ctttccgctg ctctgcctga
     1561 gtatcttggc ctttagcgac ccgtcgtagg tcttgttcac cgacgttctc ccgatgccgg
     1621 ccgaaagcca gtcggtaaag ccatattcga acccaaaccg gatattggcc tggtcgagcc
     1681 cgaaaaattc atacgccccc tggttgatgc gcccgaagtg gtgcgagatg acaaaattca
     1741 gcgccccttt cggcggcagt tcgatcgact gcccgttgat gatgcgcgtc gccttgaatg
     1801 tggcgtaagt gtaatcggtg gtattcgcct cgtcgtcgcc cagcatatcc atcaggtcct
     1861 ggccggatga aacggttgaa aagatcaata gtaaaacggg gataaggtag attttcattt
     1921 tcatggatga acagcttggt atgaataatt ttatttattg ttttccgatg atggagccgt
     1981 gctccatgat gatattctgc cggtcgtatt ctttgatcac ccctttgatg gtgacatttt
     2041 tgaacgggtt gatccctctg gcagcctggc ggaaggatgg cagcatggtc acccggatac
     2101 cttcatcgcc ggcatcgcct tttttgaagg caaaaaccac cacgatttcg tcatccacgg
     2161 cctccacccg catgatactg ccggtcactt cagctatttt cccggtgtac ctgggcccgg
     2221 caatatcagg gttcatcgaa taatcgaccc acagccgttt cgcattgacc tttacctccg
     2281 ccggccccag caccacctca gcaaccggct tttcagcgaa aaagtaggag taaccaaaat
     2341 atccccctgc ccccagcaat acaaccacag gaataattat cagcagaact ttcttcattt
     2401 caactcaaaa cttattatta actcaatact agttgttcgg tcttccggcg tcaatccagg
     2461 cctttacctg tttgatcctg caatccgaaa acttcgtccc gtttttaggc atcggcgagt
     2521 tgcccg
//
LOCUS       DAOQXS010000432         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_45910_length_2516_cov_2.996749, whole genome shotgun sequence.
ACCESSION   DAOQXS010000432 DAOQXS010000000
VERSION     DAOQXS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45910_length_2516_cov_2.996749"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PKM34_10760"
     CDS             complement(<1..553)
                     /locus_tag="PKM34_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84115.1"
                     /translation="MLLSNLALTAVVGPSVVPSIGIIRVEFILFGLTLIGVALFHKKT
                     MYVALTGLVVVLLFKLIFDPAFDFGHYILGGHGEEGEWRVLLNLMGLLFGFAILAKHF
                     EESRVPEILPKFLPDDWKGGLVLLVMVFILSAFLDNIAAAMIGGTIAMVVFKGKVHIG
                     YLAAIVAASNAGGAGSVVGDTTTT"
     gene            complement(734..>2044)
                     /locus_tag="PKM34_10765"
     CDS             complement(734..>2044)
                     /locus_tag="PKM34_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ84116.1"
                     /translation="KQPQVRLTLRTWFFSESAAGVPSLGPIRVEFILFGLTLIGVAIF
                     HRKTMYVALTGLVAVLIFKFIFDPAFHFGGHIFGSAGHEGEWRILLNLTGLLFGFAIL
                     AKIFEDSRVPDVLPGLLPDDWKGGLLLLFIVFILSAFLDNIAAAIIGGTIAMVVFKGK
                     VHIGYLAAIIASSNAGGAGSVVGDTTTTLMWIDGVSFNWVLPAYVASFSGFLIYGIVG
                     SMQQNRFQMIIKDIQPGTTIDWWRIFIVALILVGTILTNVFFDFPALGVWIAILIGSL
                     VRRPPWKELKHAWQGTVFLVALVTIASLMPVEELPPASWQSAFILGFVSAVFDNIPLT
                     KLCLDQGGYDWAILAYAVGFGGSMIWFGSSAGVALSNIYPKIRNSGKYVMNGWHVIVA
                     YVISFFVMLAILGWNPHEPHKKEHEPGTSSSINMTLNDNPDVSH"
     assembly_gap    2045..2054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          785 a          611 c          513 g          597 t
ORIGIN      
        1 aggtcgtcgt cgtgtcgccg acaacacttc ctgctccacc ggcattactg gcagccacaa
       61 tggcagccag gtatccaata tgtacctttc ctttaaaaac aaccatcgct atggttccgc
      121 caatcatggc ggctgcaatg ttatccagaa aggcagaaag gatgaaaacc attaccagca
      181 ggactaaccc gcctttccag tcatcaggaa ggaatttggg cagaatctcg gggactcttg
      241 attcctcgaa atgctttgcc aggatggcga aaccgaacag caatcccatc aggttcagta
      301 gtaccctcca ctcgccttct tcaccgtgtc cgcccaggat atagtgccca aagtcaaacg
      361 caggatcaaa aatgagttta aataacaaaa cgaccaccag ccccgtcaag gccacataca
      421 tcgtcttttt atgaaagagg gccacgccga tcagggtcag gccaaacagg atgaattcaa
      481 cacgaataat tccgattgaa ggtaccacag atggaccaac aactgcggtt aaggcaagat
      541 tgctcagcag cataagtcaa aatttcaata aagtaattgg ataaatgctt tctgatgttt
      601 ttgacaggag aaatcggatg caaaaataga gtggaatatt aatctctttt acaaaacagg
      661 taaaaaatat ttttcagcaa ataaaattcg taatgacttc tataacagta tattaactta
      721 cctgtaacag aattcaatgg ctgacatccg gattatcatt cagcgtcata ttaatggagg
      781 aggacgttcc gggttcgtgt tccttcttat gcggctcatg ggggttccaa cccagtatag
      841 ccaacatcac aaagaaactg atcacatagg ctacgatcac atgccacccg ttcatcacat
      901 atttacctga atttctgatt tttggataaa tatttgacaa agccacgcct gcagatgagc
      961 cgaaccatat catcgacccc ccgaaaccta cagcataggc aagaatagcc cagtcatacc
     1021 ccccctggtc aaggcagagt ttagtaaggg gtatgttatc aaaaacagca gatacaaatc
     1081 caagaatgaa agccgactgc caggatgccg gcggaagttc ttcaacaggc atcaaagagg
     1141 ctatcgttac cagagctacc agaaacactg ttccttgcca cgcatgtttt agctctttcc
     1201 atggcgggcg tctcaccagg cttccgatga gaatggcaat ccaaaccccc aatgccggaa
     1261 aatcgaaaaa tacattcgtc aatatcgttc caaccagaat aagcgctacg ataaaaatcc
     1321 gccaccagtc gatcgtggtt ccgggttgga tgtccttaat aatcatctga aaccggtttt
     1381 gttgcattga gccgacaata ccgtatatca agaaacctga aaatgaagcc acataagccg
     1441 gtaaaaccca attaaagctt acgccgtcga tccacatcag tgtagttgtt gtatcgccca
     1501 ctacgctgcc agcgccaccg gcattgcttg aggctatgat ggcagccagg taccctatat
     1561 gcaccttccc tttaaataca accattgcaa tggtacctcc gatgatagct gcggcaatat
     1621 tatccagaaa tgccgatagg atgaatacga tgaacaaaag caataatcct cctttccaat
     1681 cgtcgggaag caatcccggc aaaacatccg gcacgcgtga atcttcaaaa atcttggcaa
     1741 ggatcgcaaa tccaaataaa aggccggtca gattcagcaa tatcctccat tctccttcat
     1801 gcccggccga tccaaagata tgacctccaa aatggaaagc cggatcgaat atgaatttaa
     1861 atatcaaaac agccacaagt ccggttaacg ctacgtacat ggtttttctg tgaaagatgg
     1921 ctaccccgat cagagtcagg ccaaataaga tgaattcaac ccggatcggg ccgagagatg
     1981 gaaccccggc agcgctttca gagaaaaacc atgtacgtag cgttaaccgg acttgtggct
     2041 gtttnnnnnn nnnnccccag cagcgctttc agccgcctta gtaactatat tcgctaatac
     2101 catacgtatc tgattgagca ggcaaagata gaagggaaat tattctggtg aaagattgtt
     2161 gcagaaattc aaggtaataa tggtcatcct ggtcggttaa gaacatattt gaataaataa
     2221 acgttgtcgc tgatcattag cctgatcaga tacagtcccc tggagttgaa tgaaatgtca
     2281 aataccttct tataattttc ctgacttgtg cacctaaact catcatacaa aatctttctg
     2341 ccgaaggaat caaaaatgat taagctggcc ggtcccgaca atttggaaaa ttcgaaagtg
     2401 atcacaccat ttccgggatt aggaaatatc cggaacgatg gatgcccctc cggatcttca
     2461 actcccgaac aggcggtaaa atcaaaattt attgttttag acgacctggt cataca
//
LOCUS       DAOQXS010000433         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_45968_length_2514_cov_3.787312, whole genome shotgun sequence.
ACCESSION   DAOQXS010000433 DAOQXS010000000
VERSION     DAOQXS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45968_length_2514_cov_3.787312"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="purB"
                     /locus_tag="PKM34_10770"
     CDS             complement(<1..1165)
                     /gene="purB"
                     /locus_tag="PKM34_10770"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNQ84117.1"
                     /translation="MELTSSNAISPVDGRYRKQTEPLAYYFSEAAYINYRVFVEIEYF
                     IALCNIPLPQLKDFDRNMFEELRAISRNFTFADAEEVKQIEKTTNHDVKAVEYYVKKR
                     FEKLKLKQYKEFVHFGLTSQDINNTAVPYATRLALHDVLLPLVNEVEETLTRKAKEWK
                     EIPMLARTHGQPASPTRLGKEIYVFADRLKNQLSLLKTVPFGAKFGGATGNFNAHKAA
                     YPEIDWAAFGDQFVNKSLGLTRQKVTTQIEHYDNLAALFDNLRRINTILIDLCRDMWT
                     YISMDYFKQKIKADEVGSSAMPHKVNPIDFENAEGNFGMANAIFSHLAEKLPISRLQR
                     DLTDSTVIRNIGVPIGHMMIAIKSLLKGLEKVILNEEKIKADLQDNWPVIAEAI"
     gene            1342..2133
                     /locus_tag="PKM34_10775"
     CDS             1342..2133
                     /locus_tag="PKM34_10775"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNQ84118.1"
                     /translation="MNIKQNIKDLIYTLKKEQISNSSAFLLCYLKHFWNPKNELERIL
                     DNLFKSKPGAFILQVGANDGFVNDPLYKFIKKYKASGILIEPQKKPFSVPKSIYHKDD
                     IEVINCAIDRVNGIRKLYRLSFSDERWASGLSSFNYDQMVKVINSGMIEHKAKKYKIK
                     LPADKNDWISFDEVESLNFETLANNFHIEAVDLLMIDTEGFDAEIIKMFDFAKFRPLV
                     VVFEKTHLSDEDYLYCKGLLKSFTYELIEENANCIGIYNHKEWIN"
     gene            2121..>2514
                     /locus_tag="PKM34_10780"
     CDS             2121..>2514
                     /locus_tag="PKM34_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84119.1"
                     /translation="MDKLIKILVISDYNKRVSSRPEAEMFIKLAKDPQFRITVMTYPG
                     SYYYGKFRDSGIDLIDFHPDKKLDKKEVEFIRNDIMKKGYDIMHLFNGKAIINGLKAA
                     RGLKVKVVLYRGYTGNIHWYDPAAYLKVL"
BASE COUNT          671 a          526 c          603 g          714 t
ORIGIN      
        1 ggatggcctc ggcgattacc ggccagttgt cctggagatc ggctttgatc ttttcttcat
       61 tcagaataac tttttccagc cctttcagaa gggatttgat ggcgatcatc atatggccta
      121 tcgggacgcc aatattccgg atcaccgttg agtcggtcaa atcgcgttgc aggcgcgaga
      181 tcggcagctt ttcggcaagg tggctgaaaa tggcattggc cataccgaaa tttccttccg
      241 cgttttcaaa gtcaatggga ttcactttgt gaggcatggc agacgaaccg acttcgtctg
      301 ctttaatttt ctgtttgaaa taatccatcg agatatacgt ccacatgtcg cggcaaagat
      361 cgatcaggat cgtattgata cgtcgcaggt tatcgaacag cgcagccagg ttgtcgtaat
      421 gctcgatctg tgtcgttacc ttctgccggg tcaggccgag cgacttgttg acgaactggt
      481 cgccgaacgc ggcccagtcg atttcgggat aggccgcctt atgcgcgttg aaattgccgg
      541 ttgccccgcc gaatttggct ccgaacggga ccgtcttaag cagggaaagc tggttcttca
      601 gccggtcggc aaaaacgtaa atctctttgc cgagccgggt tggcgatgcc ggctgtccat
      661 gcgtcctcgc cagcatggga atttccttcc attccttcgc tttgcgggtc agtgtttctt
      721 ccacttcatt gaccagtggc agcagtacgt cgtgcagagc cagcctggtg gcatacggaa
      781 cggccgtgtt gttgatgtcc tgcgaggtca gcccgaaatg gacgaactct ttgtattgct
      841 ttaacttcag cttctcaaag cgttttttca catagtactc cacggctttc acatcgtggt
      901 tggtcgtttt ctcaatctgt ttgacctctt cggcgtcggc aaacgtgaag ttgcggctga
      961 tggcccgaag ttcctcgaac atgttcctgt caaagtcttt gagctgtggc agagggatgt
     1021 tgcacagcgc aataaagtac tcgatctcaa cgaatacgcg gtaattgata taggcggctt
     1081 ccgaaaaata ataggccagc ggctcggttt gtttgcggta cctcccatcg acgggcgata
     1141 tggcattgga tgaagtcagt tccatgattt cagcattaaa gattcaccgg gtaaatttat
     1201 atacaaacca ttcgaaatga caagtaataa ttgttttttt ttaccaaaat actacaattg
     1261 cgctctgtcg cttgtttttg aatggtttca aaagcatctc attgcgtatt ttaaaatatc
     1321 tttgttattc gattggaagc gatgaatata aagcagaata ttaaagacct tatatacact
     1381 ctgaagaaag agcagatcag caattcatct gcctttcttt tatgctatct taaacatttc
     1441 tggaatccta aaaacgaact tgaacggatc ctggataacc ttttcaaatc aaaaccaggt
     1501 gcgttcattc tccaggttgg cgccaacgac gggttcgtga atgatccgtt atataaattc
     1561 ataaaaaaat acaaggcttc aggcatcctt atcgaaccac agaagaaacc cttttcggtc
     1621 ccgaaatcta tctatcataa agatgacatt gaagtgatta actgtgcaat tgaccgggtg
     1681 aacgggatcc ggaaactgta caggctgtcc ttctcggatg agcgatgggc cagcggactg
     1741 agttcattca attacgacca gatggtgaag gttattaact ccgggatgat tgagcataaa
     1801 gcgaagaaat acaaaatcaa gcttccggcg gataaaaacg actggatcag ttttgatgaa
     1861 gtggagtccc tgaattttga aacacttgcg aataattttc atatcgaagc cgttgatcta
     1921 ttgatgattg atacggaagg atttgatgct gagattatta agatgtttga ttttgctaaa
     1981 ttcaggccct tggtggtagt atttgagaaa acccacctgt cggatgagga ttatttgtat
     2041 tgcaaaggtc tgctgaaatc gtttacctat gagttgatcg aagagaatgc gaattgtatc
     2101 ggaatttata atcataaaga atggataaat tgattaagat tcttgtgatc agcgattata
     2161 ataaaagggt ctcgtcgagg cctgaggctg agatgtttat aaaactggca aaggatcctc
     2221 aatttcgcat cactgtgatg acctacccgg gatcgtacta ctacgggaaa ttccgggatt
     2281 ccggaatcga tcttatagac tttcatccgg ataaaaaact ggataaaaag gaggttgaat
     2341 ttatccggaa tgatatcatg aagaagggtt atgatatcat gcatttattt aacggaaaag
     2401 ctattatcaa tggcctgaaa gcggccaggg gactgaaagt gaaagtagtt ctctatcgcg
     2461 ggtacaccgg aaatatccac tggtacgatc cggcagcata tcttaaagtc ctca
//
LOCUS       DAOQXS010000434         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_46186_length_2506_cov_4.539372, whole genome shotgun sequence.
ACCESSION   DAOQXS010000434 DAOQXS010000000
VERSION     DAOQXS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46186_length_2506_cov_4.539372"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1742
                     /locus_tag="PKM34_10785"
     CDS             234..1742
                     /locus_tag="PKM34_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNQ84120.1"
                     /translation="MALINSVLYWLIKKRIHQIDLFIKYPHDVQAEWFRRLITTAKDT
                     EWGKEYGYSSISNVGQYRERVPVSTYEDLKGRIDLMRQGKQNILWPTDIRWFAKSSGT
                     TDDRSKFIPVSQESLEECHYKGGKDMLSLYCNNFPNTALFSGRGLAIGGSHKISEINA
                     ESYYDGDLSAIIIQNLPFWAEFIRVPRKSTALMDNWEEKIEMMADETIPHNLTSLSGV
                     PSWMLLLLRRVLEKTGKSDIMEVWPNLEVYFHGGVNFTPYREQFRQIIRAGEMNYMET
                     YNASEGFFGLQDCSDSSELLLMLDYGIFYEFIPVSELDNDHPRACNLDEVDTETHYAM
                     VISTNAGLWRYLIGDTVKFTSINPFRIRITGRTKNFINAVGEEVIIDNAEKALAVACR
                     KCHAVVNEYTAAPLFLEKEKKVAHEWLIEFDTPPPDLEYFTETLDNALKSLNSDYEAK
                     RFHDLILLPPVIRIMAPGTFYNWLKNKGKLGGQNKVPRLSNHRTIIEEILTV"
     gene            1747..2361
                     /locus_tag="PKM34_10790"
     CDS             1747..2361
                     /locus_tag="PKM34_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNQ84121.1"
                     /translation="MHIAIAGNIGAGKTTLTELLSRHFGWEPHFEDVEANPYLNSFYE
                     DMQRWSFNLQIFFLASRFRQILNIHASGKNVIQDRTIYEDAYIFAPNLHAMSLMETRD
                     FENYSSIFELISSLIKPPDLLIYLRSTVPNLVKQIQKRGRTYEESIRLDYLKSLNERY
                     ETWISRYKQGKLLIINVDEINFADNPHDLGVVIEKIQAEIHGLF"
     gene            2368..>2506
                     /locus_tag="PKM34_10795"
     CDS             2368..>2506
                     /locus_tag="PKM34_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNQ84122.1"
                     /translation="MNCLAIIPARYGSSRFPGKPLAMINGRSMILRVCDRVKSAGVFI
                     DQ"
BASE COUNT          689 a          629 c          597 g          591 t
ORIGIN      
        1 ttttactaat gagggtgact actgtttttg caacagtagg tgacctcccc ccctacctgg
       61 ccgcctggat tccaaatatt ttctttggga tcattggcct ttacctgctg aaatcggcgc
      121 ctaaataagc cggtcttaca tctccggcgc taaagcaaaa agtttgaaca atcgctccct
      181 ttgtttacga aaatgttatt tttgaagatg aatcgcacaa gcagaaatcc gaaatggcgc
      241 taatcaattc cgtactttac tggctgatca agaagcggat ccaccagatc gacctgttca
      301 tcaaataccc gcatgatgtg caggccgaat ggttccgcag gctgatcaca acggcaaagg
      361 ataccgaatg gggcaaagaa tacggctatt cgtccatttc aaatgtcggc cagtacaggg
      421 agagggtacc tgtcagcacc tacgaagacc tgaaaggccg gattgacctg atgcgacagg
      481 gaaaacagaa tattctgtgg ccaaccgaca tccgttggtt tgccaaatcg tcgggcacga
      541 cggacgaccg gagcaaattc attccggtca gccaggaatc gctggaggag tgccattata
      601 aagggggcaa agacatgctt tcgctctatt gcaacaattt tcccaatacg gctttgttca
      661 gcgggcgtgg cctggccatc ggtggaagcc ataagatcag tgagataaac gctgagtcgt
      721 actatgacgg cgacctttcg gccatcatca tccagaatct gccattctgg gccgaattca
      781 tccgggttcc cagaaaaagc acagccctga tggataactg ggaggaaaag atagagatga
      841 tggccgatga aaccattccg cacaacctga cgagcctttc cggcgttccg tcgtggatgc
      901 tcctgctttt gcgaagggtc ctcgaaaaaa cgggcaagag cgatatcatg gaagtctggc
      961 cgaacctgga agtatatttt catggcgggg tcaatttcac gccttaccgg gaacaattca
     1021 gacagatcat ccgggccggg gagatgaatt acatggagac ctacaatgcc tctgaaggat
     1081 tcttcggtct gcaggactgc tccgactcca gcgaactgct gcttatgctg gattacggca
     1141 ttttctacga gtttatcccc gtcagtgaac tggacaacga tcatcccagg gcctgcaacc
     1201 ttgatgaagt ggataccgaa acccattacg caatggtcat ttccaccaac gccggcctgt
     1261 ggcgttacct gatcggcgat actgtcaaat tcacatccat taatcctttc aggatcagga
     1321 ttaccggcag aacaaaaaac ttcatcaacg cagttggtga ggaagtgatc atcgacaatg
     1381 cggaaaaagc gctggccgta gcctgtagaa aatgtcacgc tgttgtaaat gaatatactg
     1441 ctgcgccgtt attccttgag aaagagaaaa aggtggctca tgagtggctg atagaatttg
     1501 acactcctcc acccgacctg gaatatttca cggaaacatt ggataatgcc cttaaatcgc
     1561 ttaactcgga ttatgaagcc aaacggtttc atgatctcat tttactgccc ccggtcatcc
     1621 ggatcatggc accgggaact ttttacaact ggctgaaaaa caaaggcaag ctgggtggac
     1681 agaataaagt tccccgatta tcgaaccatc gtacaattat tgaagaaata ttaaccgtat
     1741 aacactatgc atatagctat cgcaggcaac ataggcgccg gcaaaacaac actcactgag
     1801 cttctttcaa ggcatttcgg atgggaaccg cactttgaag acgtagaagc gaatccttat
     1861 ctcaacagtt tttatgaaga tatgcagcgc tggtcgttca acctgcaaat cttcttcctg
     1921 gccagccgct tccggcagat cctgaatatt catgccagtg ggaaaaatgt catccaggac
     1981 aggaccattt acgaagatgc ctatatcttc gctcctaacc tccatgccat gagcctgatg
     2041 gaaacaaggg atttcgagaa ttacagctcc atcttcgagc tgatcagttc gctcatcaaa
     2101 cctcccgacc tgttgatcta tctccggtcg acggtcccga acctggtcaa acagatccag
     2161 aaaagaggca ggacctatga agaatcgatc cgtttggact acctgaagag ccttaacgaa
     2221 cggtatgaaa cctggatctc ccgttataaa caaggcaaac tcctaataat caatgttgat
     2281 gagataaact ttgccgacaa cccgcacgac ctgggcgtgg tgatcgagaa gatacaggct
     2341 gaaatccacg ggcttttctg ataatctatg aactgtctgg caatcattcc ggcccggtac
     2401 gggtcatcgc ggttccctgg aaaacccctg gcgatgataa atggccgctc catgatcctc
     2461 cgcgtctgcg accgggtgaa atcggcaggc gttttcatcg accagg
//
LOCUS       DAOQXS010000435         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_46652_length_2490_cov_3.631211, whole genome shotgun sequence.
ACCESSION   DAOQXS010000435 DAOQXS010000000
VERSION     DAOQXS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46652_length_2490_cov_3.631211"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..623
                     /locus_tag="PKM34_10800"
     CDS             276..623
                     /locus_tag="PKM34_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84123.1"
                     /translation="MIILAKIFHSEVQVYSIERPGFVAPEQLKKNLQRTVEIFDSEGI
                     KHQRINEKQTVLSVGFATQTLQFAVKSGAQMLAVMSVPSEEYHYFAQQDKENLLTNRE
                     GIPVLCASNQGNE"
     gene            complement(655..>2490)
                     /locus_tag="PKM34_10805"
     CDS             complement(655..>2490)
                     /locus_tag="PKM34_10805"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ84124.1"
                     /translation="KGVILSPSLYQGQEIELLLNRYFSPAFSIKDFDRLPTPFLCMGT
                     DLFTGAEVVLDSGYLPKAIRASMSIPGYFSPVDYGSYFLGDGGVVNNYPVKEVKERGI
                     DIMIGGDVQPPLRTTREEFNSLTDVIDQIITFNRGSANQIGRELTDIYVPLNTRYGII
                     DFAEYDSLIAFGERVARPFFSRFKALADSLNQIEYRAPLTFNTRPVSEVFINSVQIKG
                     YKKFAGSYFRNYFNLKEDTVFTLDEIERSIRQIYGTRFFEKVEYEVNTDGHRSDLVIR
                     VREADPGYLSAGLHYDNDYQASLLITGSFRNVLGKRTKLFADLVLGQNPRLRAFYMLD
                     NGAQPGIGARFETYSLKFDIYEREKKINRIVFSNYKTSAFINYSLKNLFNFRAGFDYE
                     YFRFRQDVAADTALEAFSDFSSYGSFFAEFSADSRDRAYFTRSGSKSELRLEYVMPLS
                     KNWSRDLFTSSFILYWKYDVSEEVAPKLVLRPGIFLGSTMSSENRPPLQHNFGFGGLN
                     PNQYVNTILPFTGLQFIQKTGYHAIILRMRLQYNFYKKLYATALFDGGAAEEIFDDVF
                     EPANFIAGYGAALGYDSFIGPLEITFMGSNRNSRPMVFLNLGFWF"
BASE COUNT          656 a          641 c          608 g          585 t
ORIGIN      
        1 gcctttaaca ccatgagtgc ctatgatggt aaggtaatat tttcaccgct ataccggaag
       61 aggccgacga cagcagctat taccttacca tcataggcac tcatggtgtt aaaggcctca
      121 ggcagaagct cctgggagcg gatattctac gcctggttac taaaattccc ggtccgacgc
      181 tggtcgtaca atcatcttca gatatagcgg cggcatatga gaaaatcatc ctcccggttg
      241 ccactcacca gtcgtatgag aacatcctca aatcagtgat catcctggca aaaatattcc
      301 attcggaagt gcaggtatat tccatcgaaa ggcccggctt cgtggccccc gaacaattga
      361 agaagaacct tcaacgaacg gttgagatct ttgattcaga aggaataaaa caccagcgga
      421 tcaacgaaaa gcagaccgtg ctttcggtgg gttttgccac acagactcta caatttgcag
      481 tcaaatcagg cgctcaaatg ttagctgtta tgtcggtgcc gtcagaggaa taccattact
      541 ttgcccagca ggacaaagaa aatcttctga caaaccggga agggattcct gtgctttgtg
      601 caagcaacca ggggaacgag tgataagggt tgtattacaa cctttccaac cctctcaaaa
      661 ccaaaacccc agattcagga acaccatcgg tctgctgttc ctgttcgatc ccatgaaagt
      721 gatctccagc gggccgataa aactgtcgta tccaagggcc gcgccataac cggcaatgaa
      781 gttggcgggt tcgaaaacat catcgaaaat ctcttcggcg gcaccgccgt cgaataacgc
      841 ggtggcatac agcttcttat aaaaattata ttgcagtctc atcctcagaa ttatagcatg
      901 atacccggtt ttctggatga actggagacc ggtgaacgga aggatcgtat tgacgtactg
      961 gttaggattc aacccgccaa aaccaaaatt atgctgcagc ggaggcctgt tctccgacga
     1021 catggtactg cccaggaaga ttcccggtcg caggaccagt tttggcgcaa cctcttcact
     1081 cacatcgtac ttccagtaaa ggataaagga actggtgaac aggtcccggc tccagttttt
     1141 cgacagcggc atgacatact ccaatctcag ttccgatttc gaaccgctgc gggtaaaata
     1201 ggccctgtcg cgggaatcgg cgctgaattc ggcaaagaac gacccgtagc tgctgaaatc
     1261 actgaaggct tccagggcgg tatcggccgc gacatcctgc ctgaagcgga aatattcgta
     1321 gtcaaatccg gcccggaaat tgaacaggtt tttcaggctg taattgataa aggctgatgt
     1381 cttataattg gagaaaacga tccggttgat ctttttctcc ctttcataaa tatcaaattt
     1441 taaagaataa gtctcgaacc tggcgcctat tcccggctgg gcaccgttat ccagcatata
     1501 gaatgcacgc agccggggat tctgtcccag gaccaggtcg gcaaacagct ttgtccgttt
     1561 gcccagcaca ttccggaagg agcctgtgat aagcaggctg gcctggtagt cgttatcata
     1621 gtgcagcccg gccgagaggt atcccggatc ggcttccctg acacgtatca caagatcgct
     1681 gcggtgacca tctgtgttca cctcgtattc aaccttttcg aaaaaacgtg tgccgtatat
     1741 ctgccgtatg ctcctttcga tctcatccag cgtgaaaacc gtatcctcct tcagattgaa
     1801 atagttcctg aagtaggatc cggcaaactt tttatagcct ttgatctgca ccgaattgat
     1861 aaaaacttcg ctgaccggcc gggtattaaa agtcaacgga gcgcgatact caatttggtt
     1921 cagggaatcg gccagcgctt tgaagcggct gaaaaacggg cgcgcaaccc tctctccgaa
     1981 agcgatcagc gagtcgtact cggcaaaatc tatgatcccg taccgggtgt tcagcggcac
     2041 atagatatcg gtaagctccc gtcctatctg gtttgcgctg ccccggttaa aggtgatgat
     2101 ctggtcgatc acgtcggtca gcgaattgaa ctcttcacgg gttgtccgga gaggaggctg
     2161 aacatcgccg ccgatcataa tatcaattcc cctctctttt acctctttga ccgggtaatt
     2221 attcaccacc cctccatcgc cgaggaagta actgccataa tcgacggggg aaaaatagcc
     2281 cgggatcgac atgctcgccc ggatggcttt gggcagataa cccgaatcca ggacgacttc
     2341 agctccggta aacagatcgg tgcccatgca gaggaaaggc gttggcagcc ggtcaaaatc
     2401 cttgatggag aatgccggag aaaaatagcg gttcagtaaa agctcaatct cctggccctg
     2461 gtaaagtgac gggctcagga taacgccttt
//
LOCUS       DAOQXS010000436         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_46761_length_2486_cov_3.710819, whole genome shotgun sequence.
ACCESSION   DAOQXS010000436 DAOQXS010000000
VERSION     DAOQXS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46761_length_2486_cov_3.710819"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="rpoB"
                     /locus_tag="PKM34_10810"
     CDS             complement(<1..427)
                     /gene="rpoB"
                     /locus_tag="PKM34_10810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065193.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ84125.1"
                     /translation="MASKKAERFNFGSSKVKAAYPDFLEIQLQSFKDFFQLETNPEDR
                     VNEGLYKVFSENFPITDARNNFVLEFLDYFVDPPRYSIDECMERGLTYSIPLKAKLKL
                     YCTDPEHEDFETIIQDVYLGMIPYMTPKGTFVINGAERVV"
     gene            complement(570..953)
                     /gene="rplL"
                     /locus_tag="PKM34_10815"
     CDS             complement(570..953)
                     /gene="rplL"
                     /locus_tag="PKM34_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNQ84126.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKDEYGIEPAAAAVAVAAP
                     GAAAGGDAPAVEEKTTFDVILKHAGQAKLGVVKLVKELTSLGLKESKELVDAAPKAIK
                     EGVTRDEAEALKKQLEEAGAEVEIK"
     gene            complement(996..1517)
                     /gene="rplJ"
                     /locus_tag="PKM34_10820"
     CDS             complement(996..1517)
                     /gene="rplJ"
                     /locus_tag="PKM34_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNQ84127.1"
                     /translation="MTKEEKDQLIDSLAETLKSSNTVYLTDISNLNSSNSTRLRALCF
                     KRNVQLQVVKNTLLKKAMERTDKNFEELYKILNGPTSVMIAESGNVPAKLIKEFRKSS
                     DKPILKGAFVQDMVFIGDDQLDILISLKSKNELIADIVALLQSPVKNVVSGLQSGGHK
                     LSGILKTLSEKEA"
     gene            complement(1541..2236)
                     /gene="rplA"
                     /locus_tag="PKM34_10825"
     CDS             complement(1541..2236)
                     /gene="rplA"
                     /locus_tag="PKM34_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNQ84128.1"
                     /translation="MAKLTKKQKESLSKFDKTKSYGLNEAVEIVKGITYTSFDASVDL
                     DVRLGVDPRKANQMVRGIVTLPHGTGKTIRVLALVTPDKESEATAAGADFVGLDEYIE
                     KIKQGWTDIDVVITMPQVMPKIGPLGKILGPRGLMPNPKTGTVTMDVGKAIKEVKSGK
                     IDFKVDKYGIIHASVGKVSFANDQLVENARELLQTIIKLKPTAAKGTYMKSVYMSSTM
                     SPGVQIEPKSLTV"
     gene            complement(2285..>2486)
                     /locus_tag="PKM34_10830"
     CDS             complement(2285..>2486)
                     /locus_tag="PKM34_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ84129.1"
                     /translation="EMAKITKGSAESNRNKVGSVTWDQVRTIAEDKMVDLNCFTVESA
                     ISMVAGTARSMGITIKGESPIN"
BASE COUNT          552 a          595 c          541 g          798 t
ORIGIN      
        1 cgacgactct ttctgcgccg ttgatcacaa aggttccttt cggggtcatg taagggatca
       61 tgcccaggta cacatcctgg atgatcgttt caaaatcttc atgctccgga tccgtgcaat
      121 aaagcttcaa tttggctttc aacggaatgg agtaggtgag accgcgttcc atgcattcgt
      181 cgatagaata ccgtggcggg tcgacaaaat aatcgagaaa ttccaggacg aaattgttcc
      241 gggcgtccgt aatgggaaaa ttctcgctga aaaccttata taatccttcg ttgacgcgat
      301 cttccggatt ggtctccaac tggaaaaagt ctttaaagga ttgtaactgg atttctaaaa
      361 aatctggata agccgctttg acttttgaag atccgaaatt gaatctttca gctttttttg
      421 atgccaaggt tttttgtttt atgccctgtc agggcggttt cgcacataat gttataatag
      481 atgatgaaaa aggggacgag cctgatccgc cagctggcgg aaacaggccc ggtaagtaat
      541 aggctaaatc gttagcctat tcatcagatc tacttaattt caacttctgc gccagcttct
      601 tccagctgtt tcttgagtgc ttctgcctca tccctggtga ctccttcttt gatggctttt
      661 ggggcggcat caacgagttc tttggattcc ttcaggccaa ggctggtcag ttcctttacg
      721 agttttacca caccgagttt agcttgtccg gcatgtttca ggatcacgtc gaacgtggtc
      781 ttttcttcca cagccggggc atctccgcct gctgctgcgc ccggagctgc aacggctacg
      841 gctgctgctg ccggttcaat accatattcg tctttaagga tctgtgctaa ctcgttgact
      901 tcctttacgg tcaagttaac caactgttct gcgaaagctt ttaaatctgc catttttatt
      961 tattttttaa tgtttcaaat ttgttttaat tctttttatg cttccttttc cgataaggtc
     1021 ttcaggattc ctgagagctt atggccgcct gactgcaggc cggaaacaac attcttaacg
     1081 ggcgactgca gcagggctac aatatccgcg atcaactcat tctttgattt gagtgagatc
     1141 agaatatcca gctgatcgtc tccgataaat accatatcct gtacaaaggc tcctttgagg
     1201 atcggtttat cggaactctt cctgaattct ttgattagtt tcgccgggac attgcctgat
     1261 tcagcaatca tcacagatgt cggaccattg aggatcttgt aaagttcttc aaaattcttg
     1321 tcagtccgct ccatcgcctt cttcaacaac gtatttttca ccacctgtaa ctgaacgttc
     1381 cttttaaaac ataaggcacg cagacgggtc gaattacttg aattcaggtt ggatatatct
     1441 gtgagataaa ctgtattgct gcttttcagt gtttccgcca atgagtcgat caattgatct
     1501 ttttcttctt ttgtcatgac tctgttttct tatttggtta ttacactgtt aaggatttgg
     1561 gctcaatttg aacgcccgga ctcatcgtac tcgacatata cacgctcttc atgtaggtgc
     1621 cctttgctgc ggtcggcttc agtttgataa ttgtctgaag caattccctg gcgttttcca
     1681 ccagctggtc gtttgcaaag gaaacttttc cgacggaggc atggataatg ccatatttgt
     1741 caactttgaa atcgatctta cccgatttga cttccttgat agccttgccg acatccatag
     1801 taaccgttcc ggtttttgga ttgggcataa ggcctcgcgg accgaggatt tttccaaggg
     1861 gcccgatctt gggcatgact tgaggcatag tgatcacaac atctatatct gtccatccct
     1921 gcttgatctt ttctatgtat tcgtcaagtc cgacaaaatc ggcgccagcc gctgtggctt
     1981 cgctctcctt atcgggcgtt accagggcca gtaccctgat cgttttccct gtcccatggg
     2041 gaagggttac gataccacgt accatctggt ttgcctttcg gggatctaca cccaggcgga
     2101 catcgaggtc aacggaagca tcaaagcttg tataagtaat ccccttgacg atttccacag
     2161 cctcattcag gccgtatgac ttcgtcttgt cgaatttcga aagggattcc ttctgttttt
     2221 ttgttagttt agccattgta ttacctcgtt atttaattct cgaattggat tcaacttatt
     2281 gcttttagtt aatcggcgat tcgcctttaa tggtgatacc catgctccgg gctgtccctg
     2341 caaccatgga tatggccgat tcgactgtaa agcaattcag gtcgaccatc ttgtcttcgg
     2401 caattgtcct gacctgatcc caggttaccg atcctacctt gttccggttc gattcggctg
     2461 accctttggt aatcttggcc atttcc
//
LOCUS       DAOQXS010000437         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_46882_length_2482_cov_3.903585, whole genome shotgun sequence.
ACCESSION   DAOQXS010000437 DAOQXS010000000
VERSION     DAOQXS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46882_length_2482_cov_3.903585"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2482
                     /locus_tag="PKM34_10835"
     CDS             <1..>2482
                     /locus_tag="PKM34_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNQ84130.1"
                     /translation="ENDKVIPAEYRAGRAQYLWKSTELSAQGLEAGPVTGLKLNLAEG
                     SNGSGDFMIRMKQVSLDELTPDTLIAGLDTVFQNRISFSTGWNDFSFFRPFDWDGVSD
                     IVVEFCYTGSGSSGEVMARAEDPGFNCGISSAGDNYGLDLDGVSDFLKLQPGTYFNSD
                     FTFETWFFKRNNNNWSRIFDFGNGPDRQNVIVALSTGTSGRLSFHVFMDGLNRSFELD
                     NPTPLYEWTHLAVRLTAHIAWVYINGNLVKLGLLQQPPNLERSINYIGRSNWNGDKAA
                     DVLLDEFRLWKTALDPATIKSHCRKGIADPAADTNLVFYYDFDEGSGNILHDRSINGH
                     HAEGFGRPGFYPVRGPEVYMGFEQDNDRPNIIFERIVSGTVDVSEVTVMDSLMDAPLQ
                     IVLFSDPDNPAFPTDTIEKWVAGFKPVYENWMVVDSVWTAPEEVIRKEMIPYYGEPFE
                     VLEEFEIGRFITPYGIGLTLGDNGFTWVYDVTDYAPLLKGMVDLSAGNQQELIDLKFM
                     LIKGTPPREVKQIDRIWGGLRSFYYKDLDDDLQMSAVTLPLRPDASQFRVKTRLTGHG
                     HNSNTGEYPHCCEWKDNEHYLRVNGQQAGAWHIFQYHDCSLNPVFPQGGTWSGAREGW
                     CPGDLVKDHDFEITDFITGSQVTIDYDITPVPEDNLGMGWGNYVTNMDLVQYGENHFN
                     VDAEVYNVITPSNVGYYSRVNPVCYGLKALLRNNGSAPLTSAKFSYGVSGGPQETFDW
                     SGSLQPHTIDTVDLPVSGDLFWIGDENHTFTVTVSEPNGTDDQYTANDTYRSKFNMPD
                     LIDIPIVLELRTNKQAYRFSLEARDISGN"
BASE COUNT          608 a          606 c          699 g          569 t
ORIGIN      
        1 agaaaacgat aaagtcatcc ctgcagaata ccgggccggt cgtgcacagt acctgtggaa
       61 atcaacggaa ctctcagccc agggtctgga agcaggtccg gtaacaggtc taaagcttaa
      121 cctggcggaa ggttcaaacg gatcgggaga ttttatgatc cggatgaagc aggtcagcct
      181 ggatgaactg acaccggata cccttatcgc aggcctggat acagtattcc aaaaccggat
      241 cagcttttca accggctgga atgatttcag tttcttccgg ccgtttgact gggatggcgt
      301 ctcagacatt gtcgtggagt tctgttatac cggttcgggg agtagcggag aagtgatggc
      361 ccgggcggag gaccccggtt ttaattgcgg aatatcatcg gccggtgata attatgggct
      421 ggatctcgac ggagtctctg atttcctaaa actgcagcca ggcacttact tcaatagcga
      481 ttttaccttc gagacctggt ttttcaagcg aaataacaac aactggagcc gtattttcga
      541 tttcggcaac gggcccgaca ggcagaatgt gatcgttgcc ctttctacgg gaaccagcgg
      601 gaggctttca tttcatgttt tcatggatgg cctgaaccgg tcgttcgagc tggataaccc
      661 aacgccactt tatgaatgga cccacctggc cgtcaggctg acggctcata tcgcctgggt
      721 ttacattaac ggaaacctgg tgaagctggg cctgctgcag caacctccaa acctggaacg
      781 aagcatcaac tatatcggga ggagcaactg gaacggcgac aaagcggcag atgtgctcct
      841 ggatgaattc cgcctctgga aaaccgccct ggacccggcg accatcaaat cgcactgtag
      901 gaaagggatc gccgatccgg cagccgatac gaatcttgtt ttttattatg attttgatga
      961 aggatcggga aatattctcc acgacagatc aataaacggc catcatgccg aagggtttgg
     1021 ccggccgggt ttttatcccg tccgggggcc ggaggtttac atgggatttg agcaggataa
     1081 tgaccgtccg aacatcattt tcgagaggat tgtatcagga accgttgatg tcagcgaagt
     1141 gactgtcatg gactcgctga tggatgcgcc gctccagatc gtgctgttca gtgatccgga
     1201 taatcctgcc tttcctacag atacgattga aaaatgggta gcgggcttca aacctgtgta
     1261 tgagaactgg atggtggttg attccgtctg gacagcgccg gaagaggtga tccgtaaaga
     1321 gatgatcccg tattacggag agcctttcga agtgctggaa gaatttgaga taggacgttt
     1381 tatcacccct tacgggatcg ggcttaccct tggtgataac ggatttacct gggtctatga
     1441 tgtgacagac tatgccccgt tgttgaaggg aatggtcgat ctgagcgccg gcaaccagca
     1501 ggaactgatc gacctgaaat ttatgctcat caaaggcact ccacccaggg aggtgaagca
     1561 gatcgaccgg atctggggcg gtctgaggtc gttctactac aaagacctgg atgatgacct
     1621 gcagatgagc gccgtcaccc ttcctttgcg tcccgatgcc agccagttca gggtgaagac
     1681 acggctgacg ggccacggcc acaacagcaa taccggtgag tacccgcact gctgcgaatg
     1741 gaaagacaat gagcactacc tgagagtaaa tggccagcag gcaggcgcct ggcatatctt
     1801 tcagtatcac gattgttcgc ttaacccggt atttccgcag gggggcacct ggtcgggtgc
     1861 acgggaagga tggtgcccgg gcgatttggt gaaagaccat gattttgaga ttacggattt
     1921 cattaccggc agccaggtta cgatcgatta tgacattacg cctgttccgg aagacaacct
     1981 ggggatggga tggggaaact atgtgaccaa tatggatctt gtccagtatg gcgagaatca
     2041 ctttaatgtg gatgccgagg tgtacaatgt gatcacaccg agcaacgtgg gctactattc
     2101 acgggtcaac ccggtgtgct acggtttaaa agccctgttg cggaacaatg gctcagcccc
     2161 gctgacttcg gcgaagttca gttacggtgt ctccggcggc ccgcaggaaa cgtttgattg
     2221 gagtggcagc ctccagcctc ataccatcga cacggtcgat ctgcccgttt ccggcgacct
     2281 cttctggatc ggcgacgaaa accatacgtt cacagtgacg gtcagcgagc cgaacggaac
     2341 ggatgatcaa tacacagcta atgatacata ccggtcgaag ttcaatatgc ccgacctgat
     2401 cgacattccc atcgtactcg aactacgcac aaataagcag gcctaccgtt ttagcctgga
     2461 ggccagggat atatccggga at
//
LOCUS       DAOQXS010000438         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47106_length_2475_cov_3.564876, whole genome shotgun sequence.
ACCESSION   DAOQXS010000438 DAOQXS010000000
VERSION     DAOQXS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47106_length_2475_cov_3.564876"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKM34_10840"
     CDS             <1..860
                     /locus_tag="PKM34_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNQ84131.1"
                     /translation="LGLVFTGLFVIVIFGLLHVKSVPDNFMNFSDLTSGTVTVSSFFT
                     AMLAAFWAYQGWVSVGFVGGEIKDARKNIPRGIIIGVFIVIAIYLLVNLTYMALLSIP
                     QLEQIYASTNQIAAVEAVRSFWGSGGALFIGLLILLTTLGCTNASILTGARPYYAMAN
                     DRLFLSSIGKLNKANVPGYSLLWQGIWASVLVLSGTFDQLTDMIIFAVFIFYGATSLG
                     VIILRRRMPDAPRPYKVWGYPVVPAIYILFCIYLCFNTIVTRPREAAIGTALILAGIP
                     VYLLLKRKN"
     gene            896..>2475
                     /locus_tag="PKM34_10845"
     CDS             896..>2475
                     /locus_tag="PKM34_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ84132.1"
                     /translation="MLLDNKTKTEDGEFFKVFDYIKNYTEKGGLDIVTGFFSIHALAL
                     MKDEVDQAEKFRLILGNLIQEESQINKVIDLLNGDPGISSTLTLSASAQKAVEFIKQE
                     KVLVKTIQRSFCHAKAYIYNDKDARKNYKIIGSSNLTDAGLGIKESSNIELNTASTGN
                     DNDFKEIKKWFRHQWDNIAQDKIELPDKTKMDVKEYLIELISNLWKPWDPIDLYYKVL
                     YELFKDDLLALSADSEFKREIAHLEETIIYKTLYPYQQKGAISLIKMLQKFNGAVLAD
                     AVGLGKTWTALAVMKYFETKGYTVVLLCPKKLRNNWEKYQSGNGSRFDKDEIEYAVRN
                     HTDLQNDRLNINYYYDLTLTKIQRKQKLLLVIDESHNLRNDKSSRYKYLVDHVLLPEH
                     TKRDVKVLHLSATPINNKLLDIRNQFKLMTKGMDDGFKDTELEIESLESIFKNAQKDF
                     AEWTSQDHRKIADFINRLPVKFEKLTDALIVARTRKLIEGEFGEMNFPKKDSPVNEYI
                     TPEKIGSLESFDDILNALR"
BASE COUNT          713 a          541 c          546 g          675 t
ORIGIN      
        1 tcctgggact ggtctttacc ggtctttttg tgatcgtgat cttcggattg ctgcatgtga
       61 agtcggttcc ggataatttc atgaatttca gcgacctgac atccggaacg gtgacggtct
      121 cttcattctt tactgccatg cttgccgcgt tctgggctta ccagggctgg gtctccgtcg
      181 ggtttgtcgg aggcgagatc aaagatgccc ggaagaatat acccaggggg atcattatcg
      241 gcgtctttat cgtgatcgcc atctacctgc tcgtcaacct gacgtacatg gccctgctgt
      301 cgattccgca actcgaacag atctatgcct ctaccaacca gatcgcggcc gtggaggcgg
      361 tccgcagttt ctggggcagc ggcggcgccc tgttcatcgg cctgctcatc ctgctcacca
      421 ccctgggctg caccaacgcc agcatcctca ccggcgcccg cccctattac gccatggcca
      481 acgaccggct cttcctcagc agcatcggga aactcaacaa agccaatgtc cccggctatt
      541 cgctcctgtg gcagggcatc tgggccagtg tgctcgttct ctccggcacc ttcgaccagc
      601 tcaccgacat gatcatcttt gctgttttca tcttctacgg cgccacctcg cttggcgtca
      661 tcatcctgcg ccgccggatg cccgatgccc cgcgtcctta caaagtctgg ggatacccgg
      721 tggtgcctgc gatctatatc ctgttttgca tctacctgtg ctttaacacg attgtcaccc
      781 ggcctaggga agccgctata ggtaccgcgc tgatcctggc cggcatcccc gtttacctgc
      841 tgctgaaaag aaaaaattaa gcgctatatt tacatctcat tttaaagtga atgctatgtt
      901 gttagataac aaaactaaaa ctgaagacgg cgaattcttt aaagtgttcg actatattaa
      961 aaattacaca gaaaaaggtg gattggatat agtcactggt ttcttttcga tacatgcact
     1021 ggcattaatg aaagatgagg tcgatcaggc tgaaaaattc agattgatcc tgggaaacct
     1081 gatacaggaa gaatcacaga tcaataaagt aatcgatttg ctgaatggtg acccgggcat
     1141 ctccagtacc ctgactttaa gtgcttcagc gcaaaaagcc gttgaattta ttaagcagga
     1201 gaaggtcctg gttaagacaa ttcagcggag tttttgccat gcaaaagctt acatatacaa
     1261 tgataaggac gcaagaaaaa actataagat aattggaagt tctaatttaa ccgatgcggg
     1321 cctcggaatt aaagaaagca gcaatattga gctgaataca gcatctaccg gtaatgataa
     1381 tgattttaaa gagataaaaa aatggttcag gcatcaatgg gacaatattg cccaggataa
     1441 aatcgaattg cctgataaaa cgaagatgga tgttaaagaa tatttgattg aactgatatc
     1501 aaacttatgg aaaccctggg acccgattga tctgtattac aaagtccttt atgagttatt
     1561 caaggatgat ttacttgccc tttctgccga ttcggaattt aaaagagaga ttgcccatct
     1621 ggaagaaaca attatttata aaactttata tccttatcag caaaaaggcg cgatcagcct
     1681 gatcaagatg cttcagaaat ttaacggcgc tgttttagct gatgccgttg gtttagggaa
     1741 aacctggaca gcccttgctg taatgaaata ttttgaaaca aaaggctata cggtggtttt
     1801 attatgccct aaaaaattga ggaataattg ggaaaaatac cagtccggta atggttccag
     1861 atttgataaa gatgaaattg aatacgcagt caggaaccat actgatttac aaaatgaccg
     1921 attgaatatc aattattatt acgatcttac tttaaccaaa atccagagga aacagaaatt
     1981 gctccttgtt atcgacgaga gccataatct tagaaatgat aagtcttctc gttataaata
     2041 tttggtagat catgttttat tgcctgaaca taccaaacgt gacgttaaag tcttgcacct
     2101 ttctgctaca ccgattaaca ataaactcct ggatatcagg aatcagttta agctgatgac
     2161 gaaggggatg gatgatggtt ttaaagatac cgaactggaa attgaaagtt tggaaagcat
     2221 ttttaaaaat gcacaaaaag actttgcgga atggaccagc caggatcatc gaaagattgc
     2281 tgattttatc aatcgtttgc ctgttaagtt tgaaaaactg acagacgctt tgattgttgc
     2341 ccgaacaaga aaactcattg aaggtgagtt cggtgaaatg aatttcccta agaaagattc
     2401 tcctgttaat gaatatatta ctcccgagaa aattggcagc ctggaatctt tcgatgacat
     2461 tctcaacgca ctccg
//
LOCUS       DAOQXS010000439         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47162_length_2474_cov_2.451013, whole genome shotgun sequence.
ACCESSION   DAOQXS010000439 DAOQXS010000000
VERSION     DAOQXS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47162_length_2474_cov_2.451013"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PKM34_10850"
     CDS             complement(<1..1207)
                     /locus_tag="PKM34_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84133.1"
                     /translation="MPDEGKDRLNFIIKADSLYGRREKVSLSLGASGEISRSLLAAAR
                     ISVAVTYAGVPEYRSWKKYGTELPSPGNAVREFENSGHILSGTVRNRDSGSSIRPDFL
                     FMSVQGKVAEFRYADIDASGRFSFILPPGSAKRNLILQPDHAAGSMILNIDPAFPYVT
                     MPSGYTKETPGENLPEGFSEMSFNFQASRIYGKTYRRDPDENMQDTLKRRRFYGIPEM
                     EVFLDDYIMLPDMEEVFFELLPGILLRKSGDGYEVRITNPLTGVFYKDSPLVMIDGVI
                     INDLNVLASLNPELVEKIEVVMTPYLIGDLILHGIVNVITRQGDFSSIDMPEYAVILP
                     YRVTEPQPVFISPDYSGRDDRESRIPDLRNTLYWNPSIITDKNAEAEISFWTSDQPGE
                     YTVIVRGISP"
     gene            1173..2075
                     /locus_tag="PKM34_10855"
     CDS             1173..2075
                     /locus_tag="PKM34_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84134.1"
                     /translation="MKFNRSFPSSGKTEKLTGSTVFLMNAAGKMYTRLYDYDTSGQNP
                     VLSYSYKREKREDTTREKTRSLPPEDWLLQPEIAGKITSLITIFQTGEGNSSFELRVE
                     GIDEDGQTGYFAKPIYGPEWSFYKTGHPLIGSLIDKNIAAAKLYARPKTRSFSGTISR
                     NQLKIDAELLDFWPFSPPAAVRFMAAGQVFVARLHMRKYHEKKNGRLAFAATVELPRE
                     FVESKNPECRKLFNGLFNNSQLIDMVMVSTGAKVHGAEDLHLIGISELADNIVSLMSK
                     NGPAGEIKAAFNKRLKFEFNSEKH"
     gene            2206..>2474
                     /locus_tag="PKM34_10860"
     CDS             2206..>2474
                     /locus_tag="PKM34_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84135.1"
                     /translation="MKRKGICLLLALMGIFAGFESLSYAEKASKDAKNKKTIRFHVVI
                     TDKCNSDFSKRLLESVKEAVNGKVFVLTEEFGVVEKDVAEREKMAL"
BASE COUNT          640 a          584 c          608 g          642 t
ORIGIN      
        1 cgggtgagat ccccctgaca ataactgtat attctccggg ctgatcagat gtccagaagg
       61 atatctcagc ttctgcgttc ttgtcagtaa ttattgaagg attccagtaa agggtgttcc
      121 tgagatcggg aatcctgctc tcccggtcgt ccctgcctga ataatcggga gagatgaaga
      181 cgggttgcgg ctcggttacc ctgtagggta gtataacggc atattcaggc atgtcgatgg
      241 aactgaaatc accctgcctc gtaataacgt tgacgatccc gtgaaggatc agatcgccta
      301 tcaggtaagg ggtcatgaca acttcgatct tctcgaccag ctcaggatta agggaggcaa
      361 gaacattaag gtcattgatt attactccgt cgatcattac caggggagaa tccttgtaaa
      421 agactcctgt caggggattg gtgatcctga cctcatagcc atcgccagat tttctgagca
      481 ggatgcccgg aagaagttcg aagaacacct cctccatatc cggaagcata atgtagtcgt
      541 cgaggaacac ttccatttcc gggataccgt aaaacctcct tcttttaagc gtatcctgca
      601 tattttcatc aggatctctc cggtaggttt ttccataaat ccttgaggcc tgaaagttga
      661 agctcatttc agagaatccc tccggcagat tctctcccgg ggtctctttc gtataacctg
      721 aaggcattgt aacataaggg aaagccggat caatgttgag gatcatgctt cccgcagcat
      781 ggtcgggctg taatatcaga tttcttttag cggaacctgg aggaagaatg aagctgaacc
      841 ttcctgaagc gtctatatcg gcatagcgga attcggccac tttgccctga acagacataa
      901 aaaggaaatc cggacggata ctgctgccgg aatccctgtt ccttacagtt cctgaaagga
      961 tatggccgct gttctcgaat tccctgacag cattccccgg agacggaagt tcggttccgt
     1021 attttttcca gctcctgtat tccggcactc cggcatatgt tactgcaacg ctgatgcggg
     1081 cagccgccag gagacttctg cttatttctc cggaggcacc caggctgagg gatacctttt
     1141 cgcgccttcc gtaaaggcta tcggctttga ttatgaaatt taaccggtct tttccctcat
     1201 ccggcaaaac ggaaaagctt accggttcaa ccgtttttct gatgaatgcc gccggaaaaa
     1261 tgtataccag gctttatgac tacgatactt ccggccagaa tccggtgctt tcttattcgt
     1321 acaaaaggga aaagcgcgaa gataccacca gagagaaaac gaggagtctg ccgcccgaag
     1381 actggctgct gcagcccgag attgccggga aaattaccag cctgatcacc attttccaga
     1441 ccggcgaagg caattcgtct tttgaattac gcgtcgaagg aatcgacgaa gacggtcaga
     1501 ccggttattt cgcaaagccg atctatggcc cggaatggtc tttttataaa accggccacc
     1561 ctcttatcgg ctctttaatc gacaaaaata ttgcggcggc aaagctttat gcgcggccga
     1621 aaacccgttc attctcagga acaatcagca gaaaccagct taaaatcgat gcggaactgc
     1681 ttgatttctg gcctttcagc ccaccggccg cagttcgctt catggctgcc gggcaggttt
     1741 ttgttgccag attacacatg aggaaatacc acgagaaaaa gaacggcaga ctggcttttg
     1801 ccgcaactgt tgagcttcct cgcgagtttg tcgagtcgaa gaaccctgaa tgtcgtaagc
     1861 tttttaacgg gcttttcaat aattctcagc tgatcgatat ggttatggtc agcaccgggg
     1921 caaaagtgca tggcgccgaa gatttacatc tgatcggaat ctcggagctg gccgacaata
     1981 tcgtcagtct gatgagcaag aacggcccgg ctggtgaaat taaagccgct tttaataaac
     2041 gtctcaagtt tgagttcaac agcgaaaagc actgactttg ttgccgtcgg ggcgagatgc
     2101 ggggacatct tacttaacta ttgtccggtg tccaatttta ttttaccggt ccagcttgtc
     2161 gtggattgat ctttttgaaa aatcgtttac ggggggaaac attccatgaa aagaaaaggt
     2221 atttgtctgc tgttggcgct tatgggtatt tttgccggct ttgaatcgtt gtcttatgct
     2281 gaaaaagcct ctaaagatgc aaaaaacaaa aagaccatcc ggtttcacgt tgtgataacg
     2341 gacaaatgca acagcgactt ctcgaagcgt ctcctcgaat ctgtcaaaga agctgtcaat
     2401 ggaaaagtat tcgttttgac agaagaattt ggcgtggtgg aaaaagatgt tgccgagcgc
     2461 gaaaaaatgg ccct
//
LOCUS       DAOQXS010000440         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47320_length_2468_cov_4.186075, whole genome shotgun sequence.
ACCESSION   DAOQXS010000440 DAOQXS010000000
VERSION     DAOQXS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47320_length_2468_cov_4.186075"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKM34_10865"
     CDS             complement(<1..1041)
                     /locus_tag="PKM34_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNQ84136.1"
                     /translation="MMTNMFKRYLLFFVLIVLIAFQSAGQGLLCEESEPFCTGSIYTF
                     PAGTTGQAQYGAYYDCLSTQPAPAWYHLLIDDPGSITIYMYSTPLVDIDFCCWGPFTD
                     PWEPCVQGLTANTVVDCSYSPAPTEYCVIPNGQTGQYYILLITNYSQQPCEITFSQTA
                     GNGSTDCTILPPPVGNNGPLCVGETLQLYADTINNAQYYWSGPNGFLSTQQNPIIPNV
                     TLNNAGAYSCVITVNGQSSDPAITNVVIYSLPGAVLTSPDTTVCPGEPAYAIFNFTGW
                     GPFKVTYNNGSNTFIQPGLYGPVDTIFLYPPVPTMYTFTKVEDLHCAKMLLFMDMYVN
                     TYPETSGTMTGSA"
     gene            complement(1038..1445)
                     /locus_tag="PKM34_10870"
     CDS             complement(1038..1445)
                     /locus_tag="PKM34_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84137.1"
                     /translation="MSAFSFGQTEVTNTSVQDLVHYDLSFNQDDFGARFSVLSHSDDN
                     YDYYAVDLTKFNDDFERVYFMVLTFTDKRIVNLDSDILRKDQTWFKSYYTSKEEEITC
                     LFNELREKTTEAGLKMSKEEKDVWMTRNNKYKK"
     gene            complement(1584..>2468)
                     /locus_tag="PKM34_10875"
     CDS             complement(1584..>2468)
                     /locus_tag="PKM34_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84138.1"
                     /translation="NLYGTTVFTLKVTDINTGCLSLADTVVVNLSGGPLGVSVTTTDD
                     TICKGESTTITAYGFGGNYGTYTITWFDGAEIVKVDYQSPSTLIVSPDDEGPHIYNVE
                     IDDGFNTSSNFLKITVFPSPVFNILGSPQIISCPTDTVRLSPDQLIPGAEYYWSNGST
                     DVSLPVVSTGIGFNSRTFDLTVTNLQGCVHKDTVTVVFDFTACTGIDEFGEDPGINLY
                     PNPSEGIINVEFLDASGLNTMQIINPYGVVVLNRPITNPGNDFLKTEINLAGLPSGIY
                     FFKALFNRNLSYHKVIIR"
BASE COUNT          686 a          530 c          587 g          665 t
ORIGIN      
        1 cgccgatccg gtcatggtgc ccgatgtctc cggataggtg tttacataca tatccataaa
       61 caaaagcatt ttcgcacaat gcaggtcttc tacttttgta aaggtataca tagttggtac
      121 agggggatag aggaagatag tatccaccgg gccgtataat ccgggctgga taaaggtatt
      181 tgagccatta ttataggtta ctttgaaagg cccccatcct gtaaaattga aaattgcata
      241 agccggttca cccgggcaga cagttgtatc gggacttgtt aagactgctc ccggcaggct
      301 gtaaatgacg acgttggtta ttgccggatc gctgctttgt ccgtttacgg taattacaca
      361 ggaataagcc cctgcgttat ttagtgttac attggggatg attggatttt gctgggttga
      421 caggaatccg ttcggcccgc tccagtagta ctgggcatta ttgatcgtat cggcataaag
      481 ctggagggtt tcgccgacac aaagcggccc gttattccct acgggaggcg gaaggatcgt
      541 acagtctgtg ctaccgttgc cggcggtctg gctgaaagtg atctcgcagg gctgctgtga
      601 ataattggta atgaggagga tataatactg accggtctgg ccgttgggaa taacacaata
      661 ctcagtcggc gcgggtgaat aactgcaatc tacaaccgta ttagccgtaa gtccctggac
      721 gcaaggctcc cagggatcgg taaacgggcc ccagcagcag aaatcgatgt cgaccagcgg
      781 ggtgctgtac atgtaaatcg taatacttcc gggatcatcg atcagcaaat ggtaccaggc
      841 aggagccggc tgggtggaaa gacagtcgta ataagcacca tattgcgcct gccctgtcgt
      901 accggccggg aaggtataaa tcgaacccgt gcagaaaggt tccgactctt cgcataacaa
      961 accctgcccg gcgctttgaa aagctataag aacgatcaga acaaaaaaaa gtaaatatcg
     1021 cttaaacatg ttcgtcatca ttttttatat ttattgttcc tggtcatcca aacatctttc
     1081 tcctctttcg acattttcaa tccggcctct gttgtctttt ccctcaactc attaaagaga
     1141 caggtaattt cttcttcctt actcgtataa taacttttaa accaggtctg gtccttccgt
     1201 aaaatatcgg aatccaggtt tacaatcctt ttatcggtga aggtcagtac catgaaatag
     1261 acccgttcaa aatcatcgtt aaattttgtc agatcaactg cataataatc ataattatca
     1321 tcggaatggc tcaaaaccga aaacctggca ccgaaatcat cctgattaaa ggaaagatca
     1381 taatgcacca ggtcctgaac agaagtattg gtaacttccg tctgtccgaa tgaaaatgcg
     1441 gacatgattg cgaaaagtat gatcaaacaa gttctcatta agggtaaaat gtttaagaat
     1501 gaatatatct ttttttaaag cgggctaaga taactatatt tttaatcaag ggtatgatat
     1561 tgaaccttat cgggtaaaaa agtttacctt ataatgacct tgtgataact taaatttctg
     1621 ttaaataaag ccttgaagaa ataaataccc gatggcaggc cagccagatt gatttcggtt
     1681 ttcaggaaat cattaccggg atttgtgatt ggccggttca gcactaccac tccgtatgga
     1741 ttgataattt gcatggtatt caaaccggag gcatccagga attcaacgtt aataatacct
     1801 tcggaagggt tggggtaaag attgattccg ggatcctcgc caaattcgtc gattcctgtg
     1861 caggcagtaa agtcaaacac aaccgtaacg gtatctttgt gaacacatcc ctggagattt
     1921 gtaacggtca ggtcgaatgt cctggaatta aatcctatgc cggttgagac gaccggcagt
     1981 gagacatcgg tacttccgtt cgaccagtaa tattctgcgc cggggataag ctgatcgggc
     2041 gaaagcctga cggtgtcggt cgggcaggat atgatctgcg gagagccgag gatattgaag
     2101 acaggtgaag ggaatactgt tattttcaga aagttggagg atgtattaaa tccatcgtcg
     2161 atctccacat tataaatatg tggtccttcg tcatccgggc taacgatcag cgtagaaggg
     2221 ctttgataat cgaccttaac aatctcggcg ccatcaaacc atgttatcgt ataggttcca
     2281 taattaccac caaacccgta tgccgtgatg gtggtgcttt cccctttaca aatcgtatca
     2341 tcggtagttg tgactgacac gcccagcggg cctccgctaa gattgaccac cactgtatcg
     2401 gccaggctga ggcaaccggt attgatatcg gtcactttta gggtaaagac agtcgttcca
     2461 tagaggtt
//
LOCUS       DAOQXS010000441         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47364_length_2467_cov_2.974710, whole genome shotgun sequence.
ACCESSION   DAOQXS010000441 DAOQXS010000000
VERSION     DAOQXS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47364_length_2467_cov_2.974710"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..789)
                     /locus_tag="PKM34_10880"
     CDS             complement(154..789)
                     /locus_tag="PKM34_10880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ84139.1"
                     /translation="MSVLVGKKAPLFEADAVVNGGEFVEKFSLEQFIGKKYVVFFFYP
                     LDFTFVCPTEILAFQEKLEEFDRRNVAVVGCSVDSKYSHWAWLNTEVKEGGIKGVKFP
                     LVSDLSKTISENYDVLAGEYEYNEDGEMDFVGDAIAYRGLFLIDKKGIVRHQLVNDLP
                     LGRSIDEAIRMVDALQCYEEYGEVCPANWHAGDEAMTATHEGVSSYLAKKH"
     gene            complement(932..2416)
                     /locus_tag="PKM34_10885"
     CDS             complement(932..2416)
                     /locus_tag="PKM34_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ84140.1"
                     /translation="MKIKIISFLSIVLLLKIPVFSQEKMVSLTLEEAIMIAKEQSADA
                     LIARHRFRMNYWQFRSFKADYLPSVNLDASLPSFNRSYGSVRQNDGTYKFQYNTYADY
                     YGALSINQKVGLTGGSVYLRSDLTRHDEFFDDSTQSEYWSTPVVVGYKQPIFKYNNYR
                     WARQLEPLKYEKAKRQYLEDVEQVAMTTTSHFFNLLLAQIEKGISEKNLHNYDTLYRI
                     AQGRYQLGKIAENDLLQLELNYLRAKASVDNASLNYENMLFRLKSYLRIKDDGDIRLT
                     PPEQTWHGTISASTALAEARKNTALSIEFQERLINAESELRYAKMNGRFDADLSLEYG
                     LNQSAQEIPDAYKSPLERQKVNLALSVPILDWGRARGEIKLKESNYELEQTSVEQEVL
                     DFEQNVFLNVMEFNMQKDQLFIAAKADTVAQKRYDVTQKRYMIGQVNDILDLNYAQID
                     NDNAKKGYFSALRNYWINYFQLRKMTLYDFRENKLLIFDIRDTM"
BASE COUNT          628 a          580 c          534 g          725 t
ORIGIN      
        1 tacctatatt tatacagcga gtccgaatga cattttcgtg acggcccatg tccagatcac
       61 tgctgaaata aggaatgcag ggaatgtata ttatcacggg gatccccagt cggtatccac
      121 tcaattgatt gggagcggga gactttacaa gttctaatgc tttttggcca ggtagctgga
      181 aacgccttca tgcgttgccg tcatggcttc atcgccggcg tgccagtttg ccgggcaaac
      241 ttccccgtac tcctcatagc actgtagtgc gtcgaccatc cggatggctt catcaatgct
      301 gcgaccgagc ggcagatcat tcacgagctg atgcctgacg atcccttttt tatcgatcag
      361 gaacaaaccc cggtaggcaa tggcatcacc cacaaaatcc atttcgccgt cctcattgta
      421 ttcatattct ccggcaagga catcataatt ctctgatatg gtttttgaaa ggtcagacac
      481 cagggggaat ttcacccctt tgatccctcc ttctttaacc tcagtattca gccatgccca
      541 gtgggaatac ttgctatcga ccgaacaacc gacaacggct acattccttc tgtcgaactc
      601 ttcaagtttt tcctggaaag ccagaatttc ggtgggacac acaaaagtaa aatccagcgg
      661 ataaaagaag aatacaacat acttcttacc gataaactgt tccagtgaga atttctcgac
      721 aaactctccg ccgttaacca ccgcatccgc ttcgaacaaa ggggcttttt tgcctactaa
      781 gactgacatt ttaatgtaat ttttaatttt taatttttaa ttcttaatta ttaattctta
      841 atctttaatt cttaatgata ttgtttgata taataaaatg aggtttggaa attttgggca
      901 aaaataatca atcctgcaaa taaacaacac gttacatggt atccctaata tcgaaaatca
      961 ataatttatt ttcacgaaaa tcataaaggg tcatttttct tagttgaaaa tagtttatcc
     1021 agtaattccg cagagcggaa aagtaccctt ttttggcatt atcgttatca atctgagcat
     1081 agttcaggtc gagaatatcg tttacctgcc cgatcatgta acgtttctgg gtcacatcat
     1141 atcttttctg agcaaccgta tcggccttgg ctgcgataaa aagctggtct ttctgcatat
     1201 tgaattccat cacattcaaa aagacgttct gctcgaaatc taatacctcc tgttcgacag
     1261 atgtttgttc caattcatag ttcgactctt tgagcttaat ttcaccccgc gccctgcccc
     1321 agtcaaggat tgggacactt agcgccaggt tgaccttttg cctttcaagc ggcgacttat
     1381 atgcatcggg aatttcctgt gcggattgat tcagtccgta ttccagcgac aggtcggcat
     1441 caaagcggcc gttcatttta gcataccgga gttcgctttc cgcattgatc agacgctcct
     1501 ggaattcgat cgacagggca gtgttcttcc tggcttccgc cagggctgtt gaagcgctga
     1561 tggtgccatg ccaggtttgt tcgggcgggg tgagccggat atcgccatca tccttgatcc
     1621 ggaggtacga tttcaaccgg aacagcatat tttcatagtt caggctggcg ttgtcaactg
     1681 aagctttagc tctcaggtag ttaagttcca gctgcaaaag gtcattttcg gcaattttcc
     1741 ccagctggta ccttccctgg gctatcctgt ataaagtatc gtagttgtgc aggttcttct
     1801 cagaaatacc cttttcgatc tgggccagga gaaggttaaa gaaatgactg gttgttgtca
     1861 tggctacctg ctcgacatct tccaggtatt gccgtttggc tttttcatat ttcagcggct
     1921 caagctgccg tgcccaccgg taattgttat atttaaagat cggttgttta tatccgacca
     1981 ccaccggtgt cgaccagtac tcggattggg tactgtcgtc gaagaactca tcatggcgcg
     2041 tcaggtccga tcgcaggtac acggaaccgc cggttaatcc caccttctgg ttgatcgata
     2101 aagcgccata gtaatcggca taggtgttgt actgaaattt gtaggtcccg tcgttttgcc
     2161 ttacactacc gtaggaacgg ttaaaagagg gcaggctggc atcgaggttg accgaaggca
     2221 ggtaatcggc tttgaatgac cggaattgcc agtaattcat cctgaacctg tgcctggcga
     2281 tcaaagcgtc ggcagattgt tcttttgcaa tcatgatcgc ttcttcaagg gtcaggctga
     2341 ccattttttc ctgtgaaaac accggtatct ttaacaacag gacgatcgac aggaaagaaa
     2401 ttattttaat cttcatacga aagaaaatta ccattattca taacgtagcg attcgacagg
     2461 atcctgg
//
LOCUS       DAOQXS010000442         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47615_length_2458_cov_3.755306, whole genome shotgun sequence.
ACCESSION   DAOQXS010000442 DAOQXS010000000
VERSION     DAOQXS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47615_length_2458_cov_3.755306"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="PKM34_10890"
     CDS             <1..2063
                     /locus_tag="PKM34_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84141.1"
                     /translation="SGGYDYSAHPGQNGISTTEQRTTLGSVVFNGLTLMCVESGGGSD
                     WETVKTWNYFGDPSVQARTAMPLQLTVSNTVVMVGIPFTTMVTTSQGPVANAMVTLSQ
                     DGLYFTGVTDDDGNVAIEQALNPGTAKLVITAFNTGTIYQDVTVTPPDGPFVTISSVE
                     INDIQGNNNQLLDYGETVYLTISLTNLGTVGAEDVSAVIATTDEFITLTDVEEDYGTI
                     PAGEVVTITSGYELSALESLPDMHIALFNLEITGTAARETWSSSFVLPGHAPVLGMAG
                     YTIDDASGNGNGRLDPGETALLQIGAVNDGSSGAFNVEGTLTCDNEFVTINGATVTYG
                     DIDAGASVMQSFEVVISPDADAGTAAQFIFDLAGDMNITGQDDIIEYVGQIPVLLLDW
                     DGNNNSADDMELCFANLEVGYDKMDVIPEERNLYTSIFVCLGTYDNNHVLSAEEGELL
                     AEYLDNGGNLYMEGADTWYYDPQFTPTPLHAMFNILGVEDGADDLEQLIGQPGSIVED
                     MFFGYNGENSYMDHIDAIPPAQVMFMNEAPSYGTMVSYDGGTYRTVGSSFEFGGLVDG
                     DMTKDDLMIRILDFFGVDGIWTGQDERAAGDISTGSYPNPFDQTTVISFKLTEPGSVM
                     VDIFDISGRNIINLFEGQATTGVTEIQWNGRDHTGKDVAPGIYFYRVDAGLSRSTGKM
                     MKIR"
     gene            complement(2065..>2458)
                     /locus_tag="PKM34_10895"
     CDS             complement(2065..>2458)
                     /locus_tag="PKM34_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84142.1"
                     /translation="KSFGVSDVHEIPEKYIEWMRGLEFFIELNSYILVHAGLNFGIDN
                     PFDDRHSMLWIKEFTVDMEKTGNRKIIHGHVPVSLDFIGVLRTSSTFKFIDLDNGVYM
                     TGKEGFGNLVALELDSLEWKIQPNADIL"
BASE COUNT          609 a          602 c          630 g          617 t
ORIGIN      
        1 gttctggcgg gtatgactat tccgctcacc ccggccagaa cgggatcagc accaccgagc
       61 aaaggacaac cttaggctct gtcgtgttca atggccttac cctgatgtgt gttgaatcgg
      121 gtggcggttc cgactgggaa acggtcaaaa cctggaacta tttcggcgat ccatcggtcc
      181 aggcccgtac cgctatgccg ctccagctta ccgtttccaa tacggttgtc atggtcggta
      241 ttccttttac cacgatggtt acgacttcgc agggccccgt tgccaatgcc atggtcaccc
      301 tcagccagga tggactttat tttacgggtg tgaccgatga tgatggcaat gtagctatcg
      361 aacaagccct caaccccgga acggcaaaac tggtcattac ggcattcaat accgggacta
      421 tttatcagga tgtgaccgtt actcctcccg acggcccctt tgtcaccatc tcatctgtag
      481 aaatcaacga tatccaggga aataacaatc agctactgga ttatggcgaa acagtttacc
      541 tgactatcag tctcaccaat ctgggtactg tcggtgcaga agatgtatcc gccgttatcg
      601 caaccaccga tgaattcatc actttaactg atgttgaaga ggattatggc actattcctg
      661 ccggggaagt ggtgaccatt accagcggat atgagcttag cgcccttgaa tccctgcccg
      721 acatgcatat cgcactgttt aatcttgaga taacaggtac agcagcccgt gagacatggt
      781 ccagcagttt cgtccttcca gggcatgctc ccgttctggg aatggccgga tatacgatcg
      841 acgatgcctc gggcaacggc aacggaaggc tcgacccggg tgaaaccgcc ctccttcaga
      901 tcggcgcggt caatgatggc tcatccggcg ctttcaatgt tgaaggtacc cttacctgcg
      961 acaatgaatt tgttacaatt aacggtgcaa cagtaactta cggcgacatt gatgccggag
     1021 cttccgtgat gcagtcgttt gaggtcgtca tcagcccgga tgccgatgca ggaaccgctg
     1081 cgcagttcat tttcgacctg gccggcgata tgaatattac cggacaagat gatattattg
     1141 aatatgtggg gcagatcccg gttcttcttc tcgactggga cggcaataac aactctgccg
     1201 acgatatgga gctgtgcttt gccaacctgg aggtcggata cgacaaaatg gatgtgatcc
     1261 ccgaagaacg caatctgtat acctccatct tcgtttgtct cggcacctac gacaataatc
     1321 atgtgttatc ggccgaagaa ggggagttgc tggcggaata ccttgacaat ggcggtaatc
     1381 tttatatgga aggggccgat acctggtatt acgacccgca gttcaccccc acgcctctgc
     1441 acgccatgtt caatatcctg ggcgtggaag atggggcaga tgacctggag cagttgatag
     1501 ggcagcccgg ttcgattgtg gaagatatgt tctttggtta taacggtgaa aacagctata
     1561 tggaccatat cgacgctatt ccgcccgcac aggtcatgtt tatgaacgaa gctccttctt
     1621 atggaacaat ggtttcctat gatggaggaa cctatcgtac cgtgggttct tcatttgaat
     1681 tcggcggtct ggtggatgga gatatgacca aagatgacct tatgatccgt atccttgact
     1741 ttttcggagt ggatgggatt tggaccggac aggatgaaag agcggcaggc gatatttcca
     1801 ccggcagcta ccccaatccg ttcgaccaaa cgacggtcat cagttttaaa cttaccgaac
     1861 ccggttccgt tatggtagat atttttgata ttagcggaag gaacattatt aacctgtttg
     1921 aaggacaagc tacaaccggt gtgacggaga ttcaatggaa tggcagggat catacaggaa
     1981 aagatgtcgc ccccggaatt tacttttaca gggtggatgc cggtttaagc cggtcgaccg
     2041 gaaaaatgat gaagatcaga taattcagag aatatcggcg ttgggctgga ttttccactc
     2101 gagagagtcc agttccagcg ctaccaggtt gccgaatcct tctttcccgg tcatataaac
     2161 gccattgtca agatcgatga acttaaaggt gctgctggtt ctgagtaccc cgatgaaatc
     2221 aaggctgacc gggacatgcc cgtgaatgat cttccggttg ccggtcttct ccatatcaac
     2281 ggtgaattct ttgatccaga gcatcgaatg gcggtcgtca aagggattat caataccgaa
     2341 attaaggccg gcatgcacca ggatgtaaga attaagctcg atgaaaaatt cgagcccgcg
     2401 catccattcg atatattttt ccggaatttc atgaacgtcg gaaacgccga aacttttc
//
LOCUS       DAOQXS010000443         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47705_length_2455_cov_3.701667, whole genome shotgun sequence.
ACCESSION   DAOQXS010000443 DAOQXS010000000
VERSION     DAOQXS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47705_length_2455_cov_3.701667"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKM34_10900"
     CDS             complement(<1..946)
                     /locus_tag="PKM34_10900"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HNQ84143.1"
                     /translation="MSNVIRISKGLTINLIGEAEKTISEIVPKTCAIKPTDFIGVFPK
                     LLVQEGDAVKAGTPLFIDKYRDKIVFTSPVSGTVKEIRRGEKRVLLEVVIENDGKDTG
                     IDFGKGDPEKMTREEITGKLLASGLWPFLRQRPYSIIAEPASKPKAIFISAFDTAPLA
                     PDYDLIVHKNGDVFQTGINAIAKLTDGAVHLNLNASTTSSGVFTNCKNVQINRFSGPH
                     PAGNVGTQIARLDPINKGEIIWYLRPQDVLHIGRLFAAGKIDATHLVAVTGSEIKRPH
                     YIKTKLGAAVEDIIKDNLSGEHVRYISGNPLTGKKVERY"
     gene            1037..2338
                     /locus_tag="PKM34_10905"
     CDS             1037..2338
                     /locus_tag="PKM34_10905"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNQ84144.1"
                     /translation="MKKAVILFISALLSFNLLPFNLVSQNEPYYDAGYIRNDNAVYRE
                     TVRTVLLYREGFELAPPLIEFGSGEKLVLSFDDLDPQYKDYRYTVVHCDADWNTSDLR
                     QMEYLEGYFEDFIEDYAYSFNTTIPYVNYTLVFPTDDLRLKKPGNYILKVFLDSDKDE
                     NIILTHRFMVYEPKVAVEGKTNNSIDLDLRYTHQQLEFRLLAGDYNMSDVHRNLHVVI
                     MQNGRWDNAIRNIQPRNYSGNIYDFTLINGLVFPAGNEFRYFDMKTLRYNTDRMQLLQ
                     YDTDGYHVYLMTDLPRASGSYISDEDINGRRLIAANETRDPYSEGDYAWVHFLLPYWP
                     PLASGNLYIAGAFSDWTYNRENLCIYNFDLKAYEAKILLKQGYYNYAYAFVANNTAVG
                     DLTWVEGSFWETKNEYSILVYYREPGDTYDKLVGVGYVNRE"
BASE COUNT          596 a          590 c          611 g          658 t
ORIGIN      
        1 tgtacctctc cacttttttg cctgtgagcg ggtttccgct gatataccgg acatgctctc
       61 cggaaaggtt atcctttata atatcttcaa ccgcggcccc taacttcgtt ttgatgtaat
      121 ggggccgttt gatttccgat ccggtaactg cgaccaaatg agtcgcatca attttcccgg
      181 cagcaaataa ccgcccgata tgcagcacat cctgcggcct gagataccag ataatttcac
      241 ctttattgat tggatcgaga cgggcgatct gtgtgcctac attaccggcc gggtgtggcc
      301 cggagaaacg gttgatctgc acattcttac agttcgtaaa tacgccggaa gaggttgtac
      361 tggcattcag gttgagatgt acggccccat cggtcagctt agctatagca ttgataccgg
      421 tctggaaaac atcaccgttt ttatggacga taagatcgta atccggggcc aggggtgccg
      481 tgtcgaaggc ggaaatgaag atggctttcg gcttcgaggc aggttcggca atgattgaat
      541 aaggccgttg cctgaggaaa ggccataagc cgcttgccag gagcttaccg gtgatctctt
      601 cacgggtcat tttctccgga tcccctttcc cgaaatcgat gccggtgtcc tttccgtcgt
      661 tctcgatgac aacctccagc aggacgcgct tttcgccgcg cctgatctct ttcaccgttc
      721 cgctgacggg tgaggtgaag acgatcttgt ccctgtattt gtcgatgaat aaaggcgttc
      781 cggctttcac ggcatcccct tcctggacca ggagtttggg aaacacaccg ataaaatcgg
      841 tcggtttgat ggcacaggtt ttggggacga tctcggaaat ggttttttcg gcttctccta
      901 tcaggttaat ggtgaggcct ttgctaatcc tgattacatt tgacatatta aaaatagtct
      961 atattttcgg cagtgcaagt gtacaaaatt ttgcgaaatc ttgcgacctt tgttgtaaat
     1021 tttacccgtg aaagatatga aaaaagccgt aatccttttc atttccgcgc tactttcttt
     1081 taacctgctg ccattcaatc tggtatcaca gaacgaaccc tattacgatg ccggatatat
     1141 ccggaatgac aatgcggttt accgggagac ggtacggact gtgctgcttt acagggaagg
     1201 atttgagctg gctccgcccc tcattgaatt tggctccgga gagaaactgg tgctgtcgtt
     1261 cgacgacctg gatccgcaat acaaggatta ccgttatacc gttgtccatt gcgatgccga
     1321 ctggaacacc tccgatctcc gtcagatgga atacctcgag ggttattttg aagattttat
     1381 cgaagattat gcttattcgt tcaatacgac cattccctat gtaaattaca cccttgtatt
     1441 cccgaccgat gatctgcgct tgaaaaaacc gggcaattat atcctgaaag ttttcctcga
     1501 ttcggataaa gatgagaata tcatcctgac ccaccggttc atggtgtatg aaccgaaagt
     1561 tgcagttgag gggaagacca acaattcgat cgacctggat ttgcgttaca cccaccagca
     1621 actggagttc aggctcctgg ccggcgatta caacatgtcg gatgtccacc ggaacctgca
     1681 tgtggtcatc atgcagaatg gccgctggga caatgcgatc cgaaatatcc agcccaggaa
     1741 ttactcggga aatatatatg atttcaccct gatcaacggc ctcgtctttc cggccggcaa
     1801 tgaattccgg tacttcgaca tgaagacgtt gcggtacaat accgaccgga tgcagttgct
     1861 gcagtatgat acggatggtt accatgttta tctgatgact gacctccccc gggccagcgg
     1921 cagttacatt tctgacgagg atattaacgg ccggaggctg atagcggcca acgaaacccg
     1981 cgatccttac agtgaaggcg attacgcctg ggtgcatttc ctactgcctt actggccgcc
     2041 gctggccagc gggaatctct acattgccgg cgccttctcc gactggacct ataaccggga
     2101 aaacctgtgc atctacaact ttgatctcaa ggcctatgag gctaaaatac tgctgaagca
     2161 gggctattac aactatgctt atgccttcgt ggccaacaat acggctgtgg gcgatttaac
     2221 atgggtggaa gggagtttct gggagacgaa gaatgaatat tcgatcctgg tctattaccg
     2281 cgagccgggc gacacatatg ataagctggt gggggtggga tatgttaaca gggaataggg
     2341 aaaatgacaa agcctaaaat aggtgtggtg atacgacttg gttcaattca attaataatt
     2401 accagttttt ttgcatccgg cttttcctgt gctacggaca ctaacagata ataaa
//
LOCUS       DAOQXS010000444         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_47796_length_2452_cov_3.583646, whole genome shotgun sequence.
ACCESSION   DAOQXS010000444 DAOQXS010000000
VERSION     DAOQXS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47796_length_2452_cov_3.583646"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKM34_10910"
     CDS             <1..528
                     /locus_tag="PKM34_10910"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNQ84145.1"
                     /translation="LILNANSEPLKNPYACGNAWFVGDFKIVADADEEIASLNGLDPA
                     RTAVIDKRFESFVAGFRPSAEPSGNIDLLSYSPNQLVYEYDAPADQLAVFSEIYYDKG
                     WNAYLDGRKSPHFRANFILRAMILPAGQHKLEFRFEPKSYYLGGNISLASSLILLLLI
                     AGYFAKEFFINRKKA"
     gene            525..1853
                     /locus_tag="PKM34_10915"
     CDS             525..1853
                     /locus_tag="PKM34_10915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ84146.1"
                     /translation="MKKVLVILYYWPPAGGSAVQRWLRFCKYLPEYGWEPTVVAPENA
                     FYTDLDKELVAEIPEKLNVLKIPIREPHRIYKAFVGLKKEENIGAYMSMKTEPKGFAR
                     IKNDLSVWIRSNFFVPDARIFWIRPVTRFLKKYLASHTFDAIVTTGPPHSIHLIGLKL
                     KASTTIPWVADFRDPWTSTDLHSALKLTRYAEKKHIRLESKVIRAADHIIAIGNDMKN
                     EFLKIGAENITVITNGYDESDIRAEYTERDRKFSIVHLGTLPATRNSSALWKVLSEKV
                     VTDKQFAKDLSIHLIGTVDYKVVQEIEALNIKDYLSITQFVKHNEGMEILRRSAVLLL
                     LINRSKNARGVVTGKIFEYMAVRRPVLLIGPVDGDAAAIIRETECGLCAEFDDEQMIR
                     TLIDHYYNLFQKNELEARPGKVSQYSRKSLAATLAQLLDKLQGDISNQVN"
     gene            1976..>2452
                     /locus_tag="PKM34_10920"
     CDS             1976..>2452
                     /locus_tag="PKM34_10920"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ84147.1"
                     /translation="MMEIWPAVAERYLDAGCGFGLKTYIFSEYFQHSTGIDFSHNVAE
                     ICKILNNKPDSLEFQITDIENLEGVGYDLITAFGLSYFNISDMGILAARIEKQVDKLL
                     NRGGVFLITTRTGPSSNAGTGWHLLNKTEIKVVKHTLRNNLRDCRIDLFEPDKSWTY"
BASE COUNT          726 a          496 c          573 g          657 t
ORIGIN      
        1 cttatcctga atgcaaacag tgagccgtta aagaatccct atgcttgcgg aaacgcatgg
       61 tttgtcggtg actttaaaat agtggctgat gccgatgaag aaattgcttc gctcaatggc
      121 ctggatcctg caagaacggc ggtcattgat aaacggtttg aatcctttgt ggccggtttc
      181 agaccgtcgg cagaaccttc cgggaacata gatctactat cctactcccc caatcaactg
      241 gtttacgaat atgatgcacc tgcggaccaa ctggccgttt tctcagagat ttattatgat
      301 aaaggatgga acgcttacct ggatggcaga aaatcaccgc atttcagggc taatttcatt
      361 ctgagagcca tgatccttcc tgccgggcag cataaacttg aattcaggtt tgaaccgaaa
      421 tcttattatt taggaggaaa tatctccctg gcaagttcat tgatattatt acttttaatt
      481 gcgggctatt ttgcaaaaga attcttcata aataggaaga aagcatgaag aaagtcctgg
      541 tgattttgta ttactggcca cctgccggcg gaagcgccgt gcaaagatgg ctcaggttct
      601 gcaaatattt gcctgaatac ggttgggaac caaccgtagt agcccctgaa aatgcttttt
      661 ataccgatct ggataaagaa ctggttgcag aaatccccga aaagctgaat gtccttaaga
      721 tcccgatccg tgagcctcac cgaatatata aagcttttgt gggattgaag aaagaagaaa
      781 atatcggggc gtatatgtcg atgaaaacag agccgaaggg ctttgccagg ataaaaaatg
      841 atctgtcggt ctggatccgg tctaatttct tcgttccgga tgcccgaatt ttctggataa
      901 ggcctgtcac cagatttctg aagaaatacc ttgcctcaca taccttcgat gcaattgtaa
      961 cgaccgggcc gcctcatagt attcacctga tcgggttaaa actcaaagct tcaactacga
     1021 tcccgtgggt tgctgatttc agagatccct ggacgagtac cgatttgcac tccgcgctga
     1081 aattgacacg gtatgctgaa aaaaagcata tcaggctgga atcgaaggtc attcgagctg
     1141 ctgaccatat cattgccatc ggaaatgata tgaagaacga gtttctgaag atcggagctg
     1201 aaaatatcac ggtgatcacg aatggatatg atgaatcgga cattcgtgca gaatatactg
     1261 aaagagatcg aaaattctcc attgttcatc ttggaacact gcctgcaacc cggaactctt
     1321 ccgcattatg gaaggttctg tccgaaaagg tcgtaaccga taagcaattt gcaaaagatt
     1381 tatcaattca tttaataggg accgttgact ataaagtggt ccaggagatt gaagccctta
     1441 acataaaaga ctatctcagc atcactcaat tcgtgaaaca taatgaaggc atggaaattc
     1501 tgcgaagatc agccgtactg ctgctcctga tcaaccggtc gaaaaatgca aggggcgtcg
     1561 taaccggcaa gatattcgaa tacatggctg tccggcgccc tgttttactg atcgggcccg
     1621 ttgacggcga tgctgcggcc atcatccggg aaacggaatg cggtttatgc gcagagtttg
     1681 atgatgaaca gatgattcgt actttgatcg atcattatta caaccttttc caaaagaatg
     1741 aattagaagc cagaccgggg aaagtgagcc agtactcgcg taagtcgctg gcagccaccc
     1801 tggctcaatt gcttgacaaa ttgcaaggtg atatttcaaa tcaggtgaat taaataaaca
     1861 ggacaggcca ttggatataa aaatttttta cgatcagaaa tacgctacat ttgaatcttt
     1921 gccgggattt caagcagaac gggaatattg catccgtatt attcaatgga tgaagatgat
     1981 ggaaatctgg ccggcggtag ctgaaaggta tctggatgcc ggttgtggat tcgggcttaa
     2041 gacttatatt ttctcagaat attttcaaca ttccacaggt attgatttca gccacaatgt
     2101 tgcagaaatt tgtaaaatac tgaataacaa accggattca ctggagtttc agataactga
     2161 cattgaaaac ttagagggtg tcggttatga tctgataacc gcgtttggtt tgagctattt
     2221 taatatttca gatatgggta ttctggctgc cagaattgaa aaacaggtcg ataaactgct
     2281 gaacaggggt ggtgtttttc tgataactac ccggacaggt ccgtcatcga acgcaggaac
     2341 cggatggcac cttttaaata aaactgaaat aaaagtcgta aaacatacgt tgaggaacaa
     2401 cctgagggat tgccggattg atctttttga accggataaa agttggactt ac
//
LOCUS       DAOQXS010000445         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_48327_length_2436_cov_3.639647, whole genome shotgun sequence.
ACCESSION   DAOQXS010000445 DAOQXS010000000
VERSION     DAOQXS010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48327_length_2436_cov_3.639647"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PKM34_10925"
     CDS             complement(<1..1309)
                     /locus_tag="PKM34_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNQ84148.1"
                     /translation="MNKFTLRIYFVSLIVLLLPALSGACLHGQTQADTSGKNIDNYRE
                     QIKRLMGFLEFSLNTLGGSDASVKEKEIIINESFLKAFMNEKVQVEDDLDENREILTY
                     KDVQAYLKDVDFFFKEAKFDFDVQDIQELKNEKGMTYFKVTANRNLNGVTVNDEIINT
                     NKTRYIEINLDDEEQVLKIASIYTTRLNESQELMTWWNGMPKEWKDFLGAAYPLGDEL
                     LLSQVDFLNDTTFLFIHEGYEIIPRETFTYIGSDSLLIIVNDTIYKEIFDTIVYGKNN
                     GIAMLKDLTRRESLDVSGNLVIADLYPADQMTELRSLNISNTSISDLFPARNLTKLVS
                     LNISGTAVSDLSPLRYNTRIRELYLDSTRVKSLTSIRDYSALEILHFSRTPVDSLHIV
                     KFLGGLKDLRFNNTRIKDLSPLADLGAIENIFLAGNSVSDLSAL"
     gene            complement(1302..2141)
                     /locus_tag="PKM34_10930"
     CDS             complement(1302..2141)
                     /locus_tag="PKM34_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84149.1"
                     /translation="MNRLVIILTIILLALTFKTEAQVVGNFVEDETDLYASTKQVNQF
                     FRRFNGEETVTGDRLYPEDKGYRDPDLRRKYFGNLFNNDNSFLKENVRNEFITYVLDK
                     KNPVYLDFHGGDWFAEVTTTFTYMGRDQMLTLFLILEKDTIGSKWVINNVYFEPFAKI
                     FADQDSLRRTGQKFLHPMSHELDFMNLVRTFEDKSSLEHYLQWKYRPDYLTLFVYEFK
                     RGNLKFKTINKVKFHFFQIDGWYFEIADFNRKGNNTGWLISALVRVNQEEKELMRKYI
                     CHE"
BASE COUNT          649 a          537 c          537 g          713 t
ORIGIN      
        1 gcagtgccga caggtcagac actgaatttc ccgcaagaaa aatgttttct atggcgccaa
       61 ggtcggccag tggcgataag tccttgatcc gggtgttgtt aaaccggaga tccttcagcc
      121 ccccgaggaa tttgacgata tggaggctgt cgaccggcgt tcggctgaaa tggagtatct
      181 caagcgctga ataatcacgg atggaggtga gtgattttac cctggtgctg tcgaggtata
      241 gttcccggat gcgggtatta taacggagag gggagaggtc ggagacggct gtgccggaga
      301 tgttaagcga aaccagtttt gtcaggttac gggcaggaaa caggtcggaa atgctcgtat
      361 tggaaatatt cagtgagcgt agctcggtca tctgatcggc agggtagagg tcagcgatga
      421 ccagattacc cgatacatcc aggctttcac gccgtgtgag gtctttcagc atggctatgc
      481 cattgttctt tccataaacg atcgtatcga aaatctcttt atatatggta tcgttgacga
      541 taatcagaag actgtcggaa ccgatataag taaaagtttc cctgggtatg atctcgtatc
      601 cttcatgaat aaacagaaat gtcgtatcgt tcaggaaatc gacctggcta agcaacaatt
      661 catcgccaag aggatatgcc gcgccgagaa aatctttcca ctctttcggc atgccgttcc
      721 accaggtcat cagttcctgg gattcattca gccgggtggt gtaaatgctg gcaattttca
      781 ggacctgctc ctcatcgtcc aggttgatct caatataccg tgttttattg gtattgatga
      841 tctcatcgtt gacggtcacc ccattcaggt tgcggttagc ggtgactttg aaataggtca
      901 ttcctttctc attcttcagt tcctggatat cctgaacatc aaaatcgaat ttcgcctctt
      961 tgaaaaagaa atccacatcc ttcagatagg cctgtacatc tttataagtc agtatttccc
     1021 ggttttcatc cagatcgtct tccacctgta ctttttcatt cataaaggct ttgagaaaac
     1081 tttcgttgat gatgatctcc ttttccttta cagatgcatc cgatcctccc agggtattca
     1141 gcgaaaattc gagaaatccc atgagacgct tgatctgttc gcggtaatta tcaatgtttt
     1201 tccctgaggt gtctgcctgg gtctgtccat gcaaacaggc tccggaaagg gctggcaaca
     1261 ggagaacaat aagggaaaca aaataaatcc tgagggtaaa tttattcatg gcagatgtat
     1321 tttctcataa gctctttttc ttcctgattt accctgacca gcgctgaaat aagccaaccg
     1381 gtattatttc ctttccggtt aaaatcagct atctcaaaat accagccatc gatctggaag
     1441 aaatgaaact tgactttgtt tatcgtcttg aatttgaggt tgcccctctt gaattcatag
     1501 acgaacaaag ttaaataatc cggacgatat ttccactgaa ggtaatgctc caggcttgat
     1561 ttatcttcaa aagttcggac caggttcata aaatcaagtt catggctcat cgggtggagg
     1621 aacttttgcc ctgttcttct caggctgtcc tggtcggcga aaattttcgc aaaaggttca
     1681 aaataaacat tattgatcac ccattttgat ccgatcgtgt ctttttcaag gatgaggaac
     1741 aatgtaagca tctgatctct tcccatataa gtaaaagtgg tggtgacctc tgcaaaccag
     1801 tcaccaccgt gaaaatcaag ataaacaggg ttctttttgt ccagaacata tgttatgaac
     1861 tcattccgga cattttcttt taaaaatgaa ttgtcgttat taaacaggtt tccaaagtac
     1921 ttcctgcgaa ggtcgggatc acggtaaccc ttatcttccg ggtaaaggcg atcaccggtt
     1981 acggtttcct cgccgttgaa cctccgaaaa aactggttga cttgcttggt gctggcatac
     2041 aggtcggttt catcttctac aaaattacca actacctgtg cctccgtctt gaacgtcaga
     2101 gccagaagta taatagtcag tataataaca agtctgttca tttttaaaca aagtatttcc
     2161 gggataagat aatgtacttc aacgactgaa gccgcagggt tcccgctaat aattaccaaa
     2221 ttggcagagg ttgtctcaac ggtcttaata attgcttctt ttggtagtcc gacgactgaa
     2281 gccgtcggtt ccaaccttca taacactgta ggaaccaacg gctttagtcg ttggagcacg
     2341 aactaatgtc aggccgtcat ttgacatatc agacttttga gacaccctca ctgcaaatat
     2401 atatttgaga atctatcttt ctgtttcagc tacacg
//
LOCUS       DAOQXS010000446         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_48622_length_2426_cov_3.709827, whole genome shotgun sequence.
ACCESSION   DAOQXS010000446 DAOQXS010000000
VERSION     DAOQXS010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48622_length_2426_cov_3.709827"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PKM34_10935"
     CDS             complement(<1..1704)
                     /locus_tag="PKM34_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ84150.1"
                     /translation="MDTARIEQTQKKLNEWSYNWTPLDKGYTDKILYVNVGSGEIKDK
                     AVPPLMKEKFIGGKGYGLKLLWDATKPDTKWDDPDNEINISSGPVGGITQYSGTGKSI
                     CVSISPQTDIPIDSNVGGFFGPYLKFSGFDAIEIQGKAEKDVIVFIDGIHHKVTISEI
                     NVDHVDSHLLAEYLTEIYADDEKDKKNIAVVSSGAAADHSLIGMLNFSFYDSRRKLVR
                     LKQAGRGGIGTVLRNKKIRAIVAKIPGVKGNLNNVVDLEAIMERGKRFNAEMRELDDS
                     QAEMRTKGTAHLTNIMNDYDLLPTHNYKYGSHPDANAIHSEVWKARFTQGIPDGCWIG
                     CNMACAKGVDNYELRTGPYKGQKVIVDGPEYENAGGLGSNCGIFNPDYIIEANFYCDT
                     YGICTISWGTIMAFVMECYENGILNEQRTGGLKLNFGNADSAMELLHQLARGEGMGMI
                     AGMGVRKMKQMFIENGWGDPQFLQDIGMENKGLEYSEYVSKESLAQQGGFAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFENKAEALHYFPMFRTWFGLVGLCKLPWNAVEPSNNAETD
                     EP"
     gene            2010..>2426
                     /gene="thiF"
                     /locus_tag="PKM34_10940"
     CDS             2010..>2426
                     /gene="thiF"
                     /locus_tag="PKM34_10940"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HNQ84151.1"
                     /translation="MLLPDQHIRDQIKTKTVGIAGCGGLGSNCAVALARVGVGKLILA
                     DFDVVETSNLNRQYFFRSQVGMPKVFALADNINRIDEGIQIDTHHLHLSAENIPVIFG
                     DCDVIVEAFDKAEMKQMIVETVLDMMPGKKLVCGVGL"
BASE COUNT          581 a          566 c          577 g          702 t
ORIGIN      
        1 cggttcatcg gtttcggcat tattggaagg ctccacggcg ttccagggca gcttgcagag
       61 gccaaccagg ccgaaccatg tgcggaacat cgggaaatag tgcagcgctt cggctttgtt
      121 ctcaaaagtc gggatctggt tgttgaccat atccatgaag atcagccagg cttcgtcgtg
      181 ctgcggccct ttgttggtca tggcaaaacc gccctgctgc gccagcgatt ctttggaaac
      241 atattcagag tattccagcc ctttgttctc cattccgata tcctgcagaa actgtggatc
      301 gccccatccg ttttcgataa acatctgttt catctttctg acccccattc cggcgatcat
      361 gcccattcct tcgccacggg ccagctggtg gagcaattcc atagccgaat cggcattgcc
      421 gaaattcagc ttcaggcctc cggtacgttg ttcattgagg attccgtttt catagcattc
      481 catcacaaac gccataatcg ttccccagct gattgtgcag atgccgtaag tatcgcaata
      541 aaaattggct tcgatgatat aatcggggtt gaagataccg cagttggaac ccagtccgcc
      601 ggcattttca tattcggggc catcaacaat caccttctgg cctttgtaag ggcccgtgcg
      661 taattcgtaa ttatcgacgc ctttggcaca ggccatgttg catcctatcc agcaaccgtc
      721 gggaatccct tgtgtgaagc gggctttcca gacttccgaa tggatggcgt tggcatcggg
      781 atgactaccg tatttgtaat tatgagtggg cagcaggtcg taatcgttca tgatattggt
      841 caggtgtgcg gtccctttcg tccgcatttc tgcctgtgag tcatcaagtt cgcgcatctc
      901 ggcattgaac cgttttccgc gctccataat ggcttccagg tcaacaacat tattcaggtt
      961 gcctttcaca cccggtatct ttgctacaat agccctgatc tttttattgc gtaaaacagt
     1021 cccaatcccg ccacgtcctg cctgtttgag ccggacaagc ttacggcggc tgtcgtaaaa
     1081 gctgaaattc agcatgccga taagggaatg gtcagcagca gctccggaag atacaacggc
     1141 tatattcttc ttatcctttt catcatctgc atatatttcg gtgagatact cggccagtaa
     1201 atgtgaatcc acgtggtcga cattgatttc gctgatggtt actttatggt ggatgccatc
     1261 aataaaaaca atgacatctt tttccgcttt accctggatc tctatggcat cgaagccgga
     1321 gaacttcagg taggggccaa agaaaccgcc gacgttgctg tcgatcggga tatcggtctg
     1381 cggagaaata gatacgcaaa tagattttcc agtaccggag tactgcgtga taccgccgac
     1441 aggaccggat gagatattta tctcgttgtc aggatcatcc cactttgtat cgggtttggt
     1501 ggcatcccag agaagcttta atccgtatcc cttacccccg ataaattttt ctttcatcag
     1561 tggcggaacc gctttatctt taatttctcc cgagccgaca ttcacgtaaa ggatcttgtc
     1621 ggtgtaacct ttatcgagcg gcgtccagtt gtacgaccat tcattcagtt tcttctgcgt
     1681 ctgttcgatt cttgcggtat ccatataaat tgatttgttg ttaatttttt agcaatgcaa
     1741 aattatgaat ttttatatcc tgatttctat taaatgcttt attttcaata tgcaagcgat
     1801 tgcataacgg tgttatggtt ataccatttc attcccaaat aattaacagg ttgtttggaa
     1861 agattatgaa ttgatccgaa taaagaataa tcgttatttt tgtgaatgaa acggccataa
     1921 gtcagaatta tatcaggaca ggcactggaa ccttggaaca ctggaacttt ggaacactgg
     1981 aaccctaaat ctttgaacta aacagcgctg tgcttttgcc cgatcaacat atccgtgacc
     2041 agataaaaac aaaaaccgtt ggtattgccg gttgtggggg cctgggctcg aattgtgccg
     2101 tagcgctggc cagggtagga gtggggaagt tgatcctggc cgacttcgat gttgtcgaaa
     2161 catctaacct gaaccggcag tattttttca gatcacaggt cgggatgccg aaggtatttg
     2221 cgctggcaga taatatcaac agaatcgatg aaggtattca gatcgataca catcacctgc
     2281 atctgtctgc agagaatatc cctgttattt tcggtgattg tgatgtgatc gtggaggcgt
     2341 tcgataaggc tgagatgaag cagatgatcg ttgaaacggt gcttgatatg atgccgggga
     2401 aaaagctggt ttgcggcgtt ggcctt
//
LOCUS       DAOQXS010000447         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_48758_length_2422_cov_3.008027, whole genome shotgun sequence.
ACCESSION   DAOQXS010000447 DAOQXS010000000
VERSION     DAOQXS010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48758_length_2422_cov_3.008027"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKM34_10945"
     CDS             <1..846
                     /locus_tag="PKM34_10945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ84152.1"
                     /translation="YDIIHLFNGKAIINGLKAARGLKVKVVLYRGYTGNIHWYDPAAY
                     LKVLSPRVDKVMCNSQSVKDQIDRQLFFDRTKTVVITKGHDPHWYSDIKPADLAEFGI
                     GQDDFVVTTTADGRKMKGTEYLLRAFELMPPGLNIRLLIMGGGEEFIRRFSGRIKNNA
                     GKVIFTGFRKDNLDIMSSCDVFISPSIKGESFQKSVAEAMNLGVCPVITDIPGNEGMV
                     IHNKCGIIIPARDAEAIKDALLDLYSDRARCREFATAAKKHISESFSLEKTVNTLAGF
                     YQSLV"
     gene            965..>2422
                     /locus_tag="PKM34_10950"
     CDS             965..>2422
                     /locus_tag="PKM34_10950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNQ84153.1"
                     /translation="MIKYWGHSAFRPMQEEIIRSALDGKDTLALLPTGGGKSVCFQVP
                     ALAMQGVCLVITPLIALMKDQVENLKKKGIKAVAVYSGMHRNEIQMAFDNSVFGDMKF
                     LYLSPERLQTEQFQQALRRMKVCLLAVDEAHCISQWGYDFRPPYLKIADIRQFLPGVP
                     VLALTATATPGVISDIQKNLAFRAENVFRKSFERKNLTYAVIREEDKNSRLLRILKKV
                     PGVGIIYVRNRKRTVETATFLRKQGIKADFYHAGLDMAARSRKQEAWIREDIRIICAT
                     NAFGMGIDKPNVRLVVHIDLPDSIEAYFQEAGRGGRDEKRSYAVTLYNAADIAKLKEN
                     LEKEYPPVDLIKAVYQSLGNYFQLPVGSGKDESFDFELYAFCRNYGYNPVVTYNALRF
                     LEREGYIAMNESLETPSRIHFEADRESIYRYQIENQSMDAFVKLLLRSYSGVFTEFVQ
                     VNEVELARRAGLKEENVVANMVKLQKAGILSYAQRP"
BASE COUNT          642 a          566 c          652 g          562 t
ORIGIN      
        1 tatgatatca ttcatttatt taatggaaaa gccattatca atggcctgaa agcggccagg
       61 ggcctgaagg tgaaagtagt tctctatcgc ggctatacag gaaatataca ctggtacgat
      121 ccggcagctt accttaaagt cctcagcccc cgggtcgata aagtgatgtg caattcgcag
      181 tcggtcaaag accagatcga caggcaacta ttttttgata gaacaaagac cgttgtcatt
      241 acgaaaggcc atgatccgca ttggtactcg gatatcaaac cggccgacct ggctgagttc
      301 ggaatcgggc aggatgattt tgtggtgact acgactgccg acggccgcaa gatgaaaggt
      361 acggaatacc tgctcagggc ttttgagtta atgcctcccg gtttgaatat ccggttattg
      421 ataatgggag ggggagaaga gtttatccgg agattcagcg gaaggataaa gaacaacgcc
      481 ggtaaggtaa tttttacggg tttccgcaag gataacctgg atattatgtc ctcctgcgat
      541 gtttttatct cgccgtccat caaaggcgaa tcatttcaaa aatcggtcgc agaagccatg
      601 aaccttggtg tttgcccggt cattaccgac attcccggca acgaaggcat ggttatccat
      661 aataagtgcg gaataataat ccctgcccgg gatgcggaag ccataaaaga tgcgctcctg
      721 gacctgtatt ccgaccgggc caggtgcaga gagtttgcca ctgcagccaa aaaacatatt
      781 tccgaaagct tcagcctgga gaaaaccgtc aacacgctgg ccggcttcta tcaaagcctg
      841 gtgtgatcag ggtgttatgt ttagtggatt ttcgtgtttt cttgatttgg tggctacaaa
      901 aggattttta gaaagggctc aacaattata aaacaaaaag gatctgaccg aaatccgcaa
      961 catattgata aaatactggg gccattccgc tttccggccg atgcaggagg agatcatccg
     1021 gtcggcactg gacggaaagg ataccctcgc tttgttgccc acgggcgggg gcaaatcggt
     1081 ctgtttccag gttccggcgc tggccatgca aggggtctgc ctggttataa ccccattaat
     1141 tgccctgatg aaggaccagg tggaaaacct gaaaaagaaa gggataaaag cagtggcggt
     1201 ctattccggt atgcaccgta acgagatcca gatggcattc gataacagcg ttttcgggga
     1261 tatgaaattc ctctacctct cgccggagcg gttgcagacc gagcagtttc agcaggccct
     1321 gcgccggatg aaagtctgcc tcctggctgt cgatgaggct cactgcatct cccagtgggg
     1381 atacgatttc cggccaccgt accttaagat cgccgatatc aggcagtttt tgcccggagt
     1441 acctgtcctc gccctgacgg ccactgctac tcccggcgtg atcagcgata ttcagaagaa
     1501 cctggccttc cgggctgaaa atgtcttccg gaagagcttc gaacggaaaa acctgaccta
     1561 tgccgtcatc cgggaggagg acaagaacag ccgcctgttg cgcatcctca aaaaggtccc
     1621 gggtgtaggt attatctatg tcaggaaccg taaacggacg gtagaaacgg ccaccttcct
     1681 gcgcaagcag gggatcaaag ccgattttta ccacgccggg ctcgatatgg cagccaggag
     1741 ccggaagcag gaggcctgga tcagggagga tatccggatc atatgcgcta ccaacgcttt
     1801 cgggatggga atcgacaagc cgaatgtcag gctggtggtc catatcgacc tgcccgacag
     1861 catcgaagcc tacttccagg aagcgggaag gggagggcgc gatgagaaaa gatcctatgc
     1921 cgttacattg tataatgcgg ccgatatcgc caagctgaaa gaaaacctcg aaaaagagta
     1981 tccgcctgtc gatttgatta aagcagttta ccaatcactg ggaaattatt tccaactccc
     2041 tgtcggaagc ggcaaagatg agagttttga ttttgagctg tatgcatttt gcaggaatta
     2101 cggttataac ccggttgtta cttataatgc actccggttc ctggaaagag aggggtatat
     2161 tgctatgaat gaatcgctgg aaactccttc acggatccat tttgaagccg accgggaatc
     2221 gatctaccgc taccagatcg agaaccagtc gatggatgct tttgtcaaac tgctgctaag
     2281 atcctattcc ggggtcttta ccgagtttgt ccaggtaaat gaggtggagc tggccaggag
     2341 agcaggattg aaagaggaaa atgtagtggc gaacatggta aaactgcaaa aagccggcat
     2401 actttcctat gcacagcgcc cg
//
LOCUS       DAOQXS010000448         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_49272_length_2405_cov_3.404255, whole genome shotgun sequence.
ACCESSION   DAOQXS010000448 DAOQXS010000000
VERSION     DAOQXS010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49272_length_2405_cov_3.404255"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PKM34_10955"
     CDS             <1..1499
                     /locus_tag="PKM34_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNQ84154.1"
                     /translation="ADVRDRLPYDLLAVDLDGTLLDSQHALPAANRDALHRAHDAGMR
                     IVLCTGRAFAETRPILEQIGLDLDATVTVFGALVTDVPTGRTLERTPIPLDTAHAATA
                     WLQRHGYPVLWLTDPNEAGTDEIIYTDYINVSDTVMPLVDFIADRTSICTNGTVHFTD
                     LSAYCPGTWQWSFSPVSVEYREGTDEFSRNPVVEFRESGPYDVTLSVTNNNGESVLTR
                     PGYIQSGGYNLPFVETFESGTLEERYWTVLNPDFDYTWTDYLIEETGNHTVRMKNYAY
                     FNMGERDQLISPYFNFSELSNVYLTFDHAYAQRFTLRDSLIIYISGECEGAWTRVWAN
                     GPDGNGIFETAPATPYEFVPLQNDDWCGLGWGADCFTIDLSDWAGENNVRIIFEGYNN
                     LGNNLFLDNITVSNTTGHQDIKPAIGSFTLFPNPGNGIFTLRSSGITGIIQLDILNSQ
                     GQMVLSESIVNHSNTYEETLDLTSLAKGIYIVRLAGSTGLQVKKLIIR"
     gene            1515..2219
                     /locus_tag="PKM34_10960"
     CDS             1515..2219
                     /locus_tag="PKM34_10960"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNQ84155.1"
                     /translation="MNLFYAPGVTAGLYTLDDEESRHCKMVLRLSEGAVIHMTDGLGN
                     LYESRIVDSKSKRITVEITGKQEAYGRMAGYLHIAVAPTKNIDRMEWFLEKSTEIGID
                     EITPLVCDHSERRQLRSERLEKVITAAMKQSYKAYRPKLNELTDFNKFIARDHPEGRF
                     IAHLEGENPILLKRAYKKDSHSVILIGPEGDFSQQELKLASEHGFQCVSLGDCRLRTE
                     TAALVACHTIRLLNQE"
     gene            complement(2229..>2405)
                     /locus_tag="PKM34_10965"
     CDS             complement(2229..>2405)
                     /locus_tag="PKM34_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HNQ84156.1"
                     /translation="AMLPRTNLSTAFVFATEDKFFAYPNNFNYYVNFYRNTFQHGGIS
                     LEEMLVPVAYLRAK"
BASE COUNT          603 a          615 c          619 g          568 t
ORIGIN      
        1 tggcagacgt gagggaccgg ttgccgtatg acctgctggc cgtcgatctg gacggaacgt
       61 tgctggactc ccaacacgcg ctgccggccg cgaaccgtga cgccttgcac cgggcacacg
      121 acgcgggcat gcggatcgtg ctgtgtaccg ggcgggcctt cgcagagacg cgcccgattc
      181 tggagcagat cgggctggat ctggacgcca cggtgacggt tttcggggcg ctggtgacgg
      241 atgtccccac cggccgcacc ctcgaacgca cacccatccc gctggacacg gcccacgcgg
      301 ctacggcctg gcttcagcgg cacggctatc cggtcctgtg gctgacggac cccaacgaag
      361 ccggcaccga cgaaattatc tacacggatt acatcaatgt cagcgatacg gtgatgccgc
      421 tggtggattt catcgctgat aggaccagta tctgcaccaa cggcaccgtt cattttaccg
      481 atctttcagc ttattgcccc ggcacctggc aatggtcgtt ttcacctgtt tcggttgaat
      541 accgggaagg taccgatgag ttttccagga acccggtcgt tgaattccgt gaatcgggtc
      601 cgtatgatgt tacattgtcg gtaaccaaca acaacggcga atcggtactg acccggcccg
      661 gctatataca gtcgggagga tataacctgc ctttcgtaga gacatttgaa tccggcaccc
      721 tggaagagcg ctactggacc gtgctcaatc ccgatttcga ttacacatgg accgactacc
      781 tgatcgaaga aacaggcaac cataccgtca ggatgaagaa ttacgcctat ttcaacatgg
      841 gtgagcgcga ccagttgatc agcccctact tcaatttcag tgaactgagc aatgtttatc
      901 tgacattcga tcatgcttat gcccagcggt ttacactgag agattcactg atcatttata
      961 tttcaggtga atgtgaaggg gcatggacac gcgtttgggc caatggtccg gacggcaacg
     1021 gtatttttga aacagcgccg gcaacaccct atgagttcgt tccgcttcaa aatgatgact
     1081 ggtgcggact gggctgggga gccgactgtt ttacgatcga cctgagcgat tgggccggcg
     1141 aaaataacgt caggattatc tttgaaggct ataacaacct gggaaacaat ctatttttag
     1201 ataatattac cgtctctaac acgaccggtc accaggatat aaagcccgcg atcggaagtt
     1261 ttaccctctt ccccaatccg ggtaatggta tctttacatt gcgttcttcc ggtataacag
     1321 gcattatcca gttggatatc cttaattcgc agggacaaat ggttctttca gaaagtattg
     1381 taaatcatag taatacttat gaagagacgc ttgacctcac atctctggcc aagggcatct
     1441 atatcgtcag gcttgcgggt agtaccggtt tgcaggtcaa aaaactgatc atccggtaat
     1501 tcagtcgtgc cgtcatgaac ctgttctatg cgcccggcgt aacagcaggc ctatataccc
     1561 tcgatgacga agaatcacgc cattgtaaaa tggtcctccg ccttagcgaa ggcgctgtga
     1621 tccatatgac agacggattg ggaaaccttt atgaatccag gatcgtcgat tcaaaaagca
     1681 aaaggatcac cgttgagatt accggaaaac aagaagccta tgggcgcatg gccggctatt
     1741 tgcatattgc cgtagccccg acaaaaaata ttgaccgtat ggaatggttc ctggagaaat
     1801 caaccgagat cggtattgat gagatcaccc cgctggtgtg cgaccattcc gaacgccggc
     1861 agctccggag tgagcggctt gagaaagtga tcacggctgc catgaaacaa tcttacaaag
     1921 cttacagacc gaagctgaac gaattgaccg attttaacaa atttatcgcc cgggatcatc
     1981 ctgagggccg gtttatagcc catctcgaag gagaaaatcc gattttatta aaaagagcct
     2041 ataaaaagga ttcccattct gttattttga tcgggcccga aggtgatttc agtcaacagg
     2101 aattgaaact ggcttcagag catggttttc agtgcgtttc gctcggcgat tgccgactgc
     2161 gcaccgaaac cgcagccctg gttgcgtgcc atacgatacg gctgttaaac caggaatgat
     2221 cccttcccct atttcgccct gagataagct acaggtacca gcatctcttc cagagagatc
     2281 ccgccatgct gaaaggtgtt cctgtaaaaa ttaacataat agttgaagtt gtttggatag
     2341 gcaaagaact tgtcctcggt ggcaaacaca aacgcagtgc tgaggttggt ccttggcaac
     2401 atcgc
//
LOCUS       DAOQXS010000449         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_49403_length_2401_cov_3.549446, whole genome shotgun sequence.
ACCESSION   DAOQXS010000449 DAOQXS010000000
VERSION     DAOQXS010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49403_length_2401_cov_3.549446"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PKM34_10970"
     CDS             complement(<1..1086)
                     /locus_tag="PKM34_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ84157.1"
                     /translation="MNFLGAQDIKAIRNFMKMLYDVAPAGQEPRYLLLFGDASFDYKD
                     RIQDNSNLVPTWEDEESLVIVYSIASDDYYGFLDGPGDNLLDIGIGRLPVRTVEQATV
                     AVDKIIHYATNTQEVMKEWRNYICFVADDEDGNLHIRQAEEMATFIDTTYGAYNIDKI
                     YIDAFPQVSTPGGQRAPDVNRALNNRIDKGCLVMNYTGHGGEVGWGHERFLENTDINS
                     WTNYDHMPIFITATCEFSRYDDPERISAGEYTFLNPLGGAIAMFTTARATFGGSNFNL
                     NTALFDYMFEKDNGEHYRFGDLIRLAKNKGGVDANDKKFILLGDPALHLAYPEHEVVT
                     TRINGEDISGVPDTLQALRKITIEGEVR"
     gene            complement(1058..>2401)
                     /locus_tag="PKM34_10975"
     CDS             complement(1058..>2401)
                     /locus_tag="PKM34_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNQ84158.1"
                     /translation="EILIAFDLVVSAGGSLPDTRQTDGNYIQNSVLATGTWYKLAVTE
                     TGIYRISYPDLQNFGINPSGIDPRNIRIYGNGGGMLPESLNEFRHDDLAENRIFVSGE
                     SDGKFDPDDYILFYGESPHEWKYQSFSQAFHHFQNIYSDSTYYFLTTGLGPGKRVVSV
                     QSSNEPPDVYISRFTDYDYHEVDLINLTNIGRVWYGEVFDVNTIYEFPFSFPNIDLES
                     QAYFRAYAAAKSESVTSFSFYDNGSKILSSNISGIPPSSNTYARAYIGSNWFTPSSSS
                     LIIKAEFNKNISSSIGWLNFLELNVTRQLIFTGGQMSFREPSAASPGTIAEFALGNAG
                     NAVQIWNVTDPTSASRIETITSGNGKLFRIAQDTMSEFIAFDGSNYLKPVYAGITGNQ
                     NLHGSGPQDMIIITHESFREQADRLAGFHREHDGLSVLVAGIEEVYNEFSWCSGY"
BASE COUNT          635 a          594 c          553 g          619 t
ORIGIN      
        1 cctgacttcc ccttcgatcg ttatttttct cagtgcctgg agcgtatcgg gcacgcccga
       61 aatatcctcc ccgttgatac gggtagtcac tacctcatgt tccggatatg ccagatgcaa
      121 ggcaggatca cccagaagga tgaatttttt atcgttggca tccacacccc ccttgttttt
      181 agccagccgg atcagatccc cgaaacggta atgctccccg ttatcttttt caaacatata
      241 gtcaaaaaga gcagtattaa gattgaagtt ggaaccgcca aaggttgccc gggcagtggt
      301 aaacatggca atagcccctc ccaggggatt cagaaaggtg tattccccgg ccgaaatcct
      361 ttccggatca tcatagcggc tgaactcgca ggtagcggta atgaagatgg gcatatggtc
      421 gtaattcgtc caggaattaa tatcggtgtt ttccaggaac ctctcgtgcc cccagccgac
      481 ctctcccccg tgccctgtgt agttcatcac caggcagcct ttatcgatcc tgttgttcaa
      541 tgcgcggttg acgtccggcg ccctttggcc accaggcgtc gaaacttgtg gaaaagcatc
      601 gatatagatt ttatcaatat tataggcacc gtaggttgtg tcgatgaagg ttgccatctc
      661 ttccgcctgc cggatatgca aattaccatc ttcatcgtcg gcgacaaagc agatgtagtt
      721 ccgccattcc ttcatcactt cttgcgtatt ggtggcataa tgaatgatct tgtcgaccgc
      781 taccgtggcc tgttcaaccg tacgtacagg caaccggccg atcccaatat ccagaaggtt
      841 atccccgggg ccatccagaa aaccgtaata atcatccgaa gctatagaat atacaatgac
      901 cagcgattcc tcgtcttccc atgttggcac cagattggag ttatcctgaa tcctgtcctt
      961 gtaatcaaaa gaagcatctc caaacaacag gagataccgg ggctcctggc cggcaggggc
     1021 aacatcgtaa agcattttca taaagttgcg gatggcttta atatcctgag caccaagaaa
     1081 attcattgta aacttcttca attccagcga ctaaaaccga taagccatcg tgctccctgt
     1141 gaaagccggc cagtctgtcg gcctgctccc tgaacgactc gtgcgtgatg atgatcatat
     1201 cttgaggtcc ggagccatga aggttctgat tgcccgttat gccggcatac acaggcttaa
     1261 gataatttga accgtcgaaa gcaataaatt cggacattgt atcctgggct atgcgaaaca
     1321 actttccgtt tccggatgtg atcgtttcaa tccttgaagc actggtcgga tcggtcacat
     1381 tccagatctg gactgcatta ccggcattgc caagggcaaa ctccgctatt gttcccggag
     1441 aagcggcgga gggctcacga aacgacattt gtccgccggt aaaaatgagt tgacgggtaa
     1501 cgttcaattc caggaaattc agccacccga ttgaactgga tatgttttta ttaaattccg
     1561 ctttgataat caggctggac gatgaagggg taaaccagtt gctgcctata tatgccctgg
     1621 cgtatgtatt ggaagatggc ggaataccgc tgatgttact gctgaggatc ttacttccgt
     1681 tatcataaaa actgaaagat gtaacagatt ccgattttgc cgccgcatag gcccggaaat
     1741 aggcctggct ttccaggtca atattaggaa atgaaaacgg aaactcataa atggtattga
     1801 catcgaaaac ctcaccatac cataccctgc cgatattggt caggttgata agatcaacct
     1861 catggtagtc gtaatccgta aacctggaaa tataaacatc aggcggttca ttcgaagact
     1921 gaacggagac aacccttttt cctggcccaa gaccggttgt taagaaataa taagttgaat
     1981 cgctgtatat gttttgaaaa tggtgaaacg cctggctgaa cgactggtat ttccactcat
     2041 ggggagattc gccgtaaaat aaaatataat catcgggatc aaattttcca tccgattcgc
     2101 ccgatacgaa tatccggttc tcagcaaggt catcatgcct gaattcgttg agcgactcgg
     2161 gcagcatacc accgccgtta ccataaatcc ggatatttcg gggatctatt cctgaaggat
     2221 tgataccgaa attctgaagg tcaggatatg atatcctgta tataccggtc tcagtaacag
     2281 ccagtttgta ccacgtcccg gtcgcaagga ccgaattctg gatataattg ccgtccgttt
     2341 ggcgtgtgtc aggtaatgat cccccggctg aaacaacaag atcgaaagcg atcagaatct
     2401 c
//
LOCUS       DAOQXS010000450         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_49540_length_2397_cov_3.187447, whole genome shotgun sequence.
ACCESSION   DAOQXS010000450 DAOQXS010000000
VERSION     DAOQXS010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49540_length_2397_cov_3.187447"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKM34_10980"
     CDS             complement(<1..408)
                     /locus_tag="PKM34_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNQ84159.1"
                     /translation="MEQEKFYYDNRIVKYFAYATLFWAIIGMLVGLLVALQFIFPSLN
                     FDLAYTTFSRIRPVHTNAVIFAFVGNGIFTGVYYSLQRLCKARMFNDRLSYIHFWGWQ
                     LIIVLAALTFIFGITTSKEYAELEWPVDILVALI"
     gene            576..791
                     /locus_tag="PKM34_10985"
     CDS             576..791
                     /locus_tag="PKM34_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84160.1"
                     /translation="MAFTIWNFLNDVFLIIKKHPDGRCIFVVELTGTDSPDEGGQEAD
                     GNQQTDGNEDKDDVHGWLSEIGSGKQI"
     gene            complement(861..>2397)
                     /locus_tag="PKM34_10990"
     CDS             complement(861..>2397)
                     /locus_tag="PKM34_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ84161.1"
                     /translation="YQALSFERDYKSYFPVAVTILDDDGKESTIPLKNLRPGNRIMVR
                     NQELVPADAILVSGQGHIDYSFVSGESTPVHKQVKDRIFAGGRQVGGMIELIVEKEVV
                     QSYLTELWNQDLHRKTNSKGLNPVVDSVSRYFTLVIITISLGTGIFWWMTDPSQAMNA
                     FTSVLIVACPCALALTIPFTLGNTMRIFGRWGFYVKKTEVLEDLSRIDTIVFDKTGTI
                     TQNDVFHVDFSQVELTEKEMALVRSLARHSSHPLSQAIYGSIKTGNLLPVEDFSEQPA
                     LGLSGFVENTAIRIGSELYITGLEEETKNYSSRVFVSIEGQIRGFITIRNEYRPGMEK
                     VIGALRNRHELHLLSGDNDAEKGHLMPVFGDERSLHFRQSPAGKLEYIKNLKDAGKKI
                     LMVGDGLNDAGALKESHVGITIADNVYHFSPACDGILDADRFGRLNTFISFSRTAMKI
                     VYAGFTLSFLYNIIGISFAAAGLLTPLLSAILMPLSSVSAVAFATLATWLLAKRRLRV
                     LYI"
BASE COUNT          588 a          582 c          601 g          626 t
ORIGIN      
        1 gatcagggct accaggatgt cgaccggcca ttccagttct gcatactcct tgctggtggt
       61 aataccgaaa ataaaggtca gtgcggccag aacgatgatc agttgccatc cccagaagtg
      121 gatatagctg agtctatcat tgaacatacg ggctttgcaa aggcgttgaa gcgaatagta
      181 aacaccggtg aatatcccgt tgccgacaaa ggcaaatatc acggcattcg tatgcaccgg
      241 cctgatcctg ctgaatgtgg tataggccag gtcgaaattg agtgatggga aaataaattg
      301 caaagccaca agcaatccga caagcattcc gataatcgcc cagaacagcg tggcataggc
      361 gaaatacttg acgatcctgt tgtcgtagta aaacttttcc tgttccataa attgttgatt
      421 gttattggtt tttggttata agttattagt tcttagttcc gggttccagg ttattaatta
      481 gtggtttttg gtcaggtgat tgtttttttg ctttgttttt tttgtttttg ttttgtttct
      541 tttttttgat ttcgaatttg acttttgttt ggcatttggc atttaccatt tggaattttc
      601 tcaatgatgt ttttctcatc atcaaaaagc accctgacgg acggtgtata ttcgtcgtcg
      661 aactgaccgg tacggatagt ccagatgaag gcggccagga agccgatggc aaccagcaga
      721 ctgacggcaa tgaggataaa gatgacgttc acggatggtt aagtgaaatt ggttccggaa
      781 aacaaatata aatgagaata aggtaggatg acgggatcag tctgccgaaa tatgtatgtt
      841 tattgatatt tagaagggtt ctaaatatat aatactctca gccgccgttt tgccagcagc
      901 caggtggcca atgtcgcaaa agccacagcc gagaccgagc ttagcggcat caggatggcg
      961 gagagcagcg gagtgagcag gccagccgcc gcaaacgata tgccgatgat gttgtaaaga
     1021 aatgagaggg tgaaaccggc gtaaacaatc ttcatggcgg tccggctgaa gctgatgaag
     1081 gtattcagcc tgccgaaccg gtcggcatca aggataccgt cgcaggcagg ggagaagtgg
     1141 tagacattgt cggcaatagt aatcccgaca tggctctcct tcagggcgcc ggcatcgttc
     1201 agcccgtcgc ccaccatcag tatcttcttc ccagcatctt tgagattttt gatgtattcg
     1261 agcttgccgg ccggcgactg gcggaaatgc aggcttcgtt catctccgaa aacaggcatc
     1321 aggtggccct tctctgcatc attatcaccc gaaagcaggt gtaattcatg cctgtttctc
     1381 agagcgccga tcaccttctc cataccaggc cggtattcgt tgcggatcgt gataaagccc
     1441 ctgatttgcc cttcaatcga gacaaacacc cgcgacgaat aatttttcgt ctcctcttcc
     1501 agcccggtaa tatataattc cgatcctatc cttatggctg tattctcaac aaatccgctc
     1561 agccccaggg ccggctgctc gctaaaatcc tctaccggta gcaggtttcc ggtcttaata
     1621 ctgccgtaaa tagcctggct cagcggatgt gacgagtgcc gcgccagcga cctcaccagg
     1681 gccatctcct tttctgtcag ttcaacctgg ctgaaatcga catggaagac atcgttctgg
     1741 gtaatcgttc ccgttttatc gaaaacgatg gtgtcgatcc gtgaaagatc ttccagtact
     1801 tccgttttct tcacataaaa accccatctt ccgaagatcc gcatggtatt gcccagggta
     1861 aaaggaatgg tcagcgccag ggcgcagggg caggccacga tcagcaccga ggtgaaggca
     1921 ttcatcgcct gcgaaggatc ggtcatccac cagaatatac cggttccgag acttatggtg
     1981 atgataacca gcgtaaagta gcggctgacg ctgtcgacca ccgggttcag acctttgctg
     2041 ttcgttttgc ggtgcagatc ctggttccag agctcggtaa gataactctg taccacctct
     2101 ttctcaacga tcagctcaat catcccgccg acctgtctgc cgccggcaaa gatcctgtct
     2161 ttcacctgtt tgtgaaccgg agtggattcg ccgctgacaa agctgtaatc gatatgccct
     2221 tggccggaaa caaggatagc atcagccggc accagctcct ggttccggac catgatccgg
     2281 ttgccgggtc tgagattctt cagcggaata gtcgattcct tcccatcatc atccagaatg
     2341 gtgaccgcca cggggaaata ggatttgtaa tcacgttcga acgacagcgc ctggtag
//
LOCUS       DAOQXS010000451         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_49848_length_2387_cov_4.828902, whole genome shotgun sequence.
ACCESSION   DAOQXS010000451 DAOQXS010000000
VERSION     DAOQXS010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49848_length_2387_cov_4.828902"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKM34_10995"
     CDS             <1..437
                     /locus_tag="PKM34_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNQ84162.1"
                     /translation="RHYGKKAVLLVILAGLMIVISDQTSVHLFKNVFLRLRPCHEPSL
                     AGMVHLVDGRCGGKYGFISSHAVNSFALATYLSFLLGKRIRYFTPLILFWAFLLSYSR
                     IYLGVHYPGDVIVGGLWGAGIGAAIFIISIRIPFHPSSQPSP"
     gene            complement(391..852)
                     /locus_tag="PKM34_11000"
     CDS             complement(391..852)
                     /locus_tag="PKM34_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ84163.1"
                     /translation="MKNRQITNKEHQDAIIRKCQFCHVAMTTPDGKPYVIPMNFGYDG
                     EFVYFHGSAKGKKADILKINPDVCIAFSTDHELRYVNEEVACSWSMRYKSVLVYGKAE
                     FIEDAGSKIECLNRIMAQYADRDFEYNEPAVREVMVFRVKVEKMDGRVYGY"
     gene            complement(827..1207)
                     /locus_tag="PKM34_11005"
     CDS             complement(827..1207)
                     /locus_tag="PKM34_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNQ84164.1"
                     /translation="MKVLIRLLVYTCAVLLTAWLLPGVSVENFTTAVMVAISLAILNT
                     FVRPILVFLTIPVTIVTLGIFLLVINALIILFIGKVIPGFTVDGFWWALLFSVILSIV
                     ASLFGTSSTTIVRRNEYEKPADNQ"
     gene            1277..2218
                     /gene="trxB"
                     /locus_tag="PKM34_11010"
     CDS             1277..2218
                     /gene="trxB"
                     /locus_tag="PKM34_11010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNQ84165.1"
                     /translation="MEHIHCLIIGSGPAGYTAAIYAARADLHPVIYEGIQPGGQLTTT
                     TEVENFPGYPDGIMGPQMMEDMKKQAARFGTDIRFGIVTEVDLSKRPFTVKADDGKTM
                     LADTLIIATGATAKYLGLESEERFMGAGVSACATCDGFFYRGKDVAIVGGGDTAAEEA
                     SYLAKLCRTVHLIHRREELRASKAMQKRVTTTPNIVLHWNCVVKEIVGTEEGFNKAVT
                     GVRIENIKTGEQELLPVDGYFSAIGHKPNSDLFKGVLDMNEVGYLITKPDSTETNVPG
                     VFACGDVQDWKFRQAVTAAGSGCMAALEAERFLAAQE"
BASE COUNT          579 a          626 c          607 g          575 t
ORIGIN      
        1 accggcatta cgggaaaaag gcggtgctac tggtgatcct cgccggtctg atgatagtga
       61 tcagcgatca gacctccgtt caccttttta agaatgtttt cctgaggctc aggccctgcc
      121 acgagccatc gctggcagga atggtacacc tggtcgacgg gcgctgcgga gggaagtacg
      181 gtttcatctc ttcccatgcc gtaaactcct ttgccctggc tacttacctg tcgtttttac
      241 tgggaaaaag aatccggtac tttacgccgc tgatcttatt ctgggctttt ctactgagct
      301 acagccggat atacctgggg gtgcattatc cgggcgatgt gattgtcggc ggcctctggg
      361 gagccggcat cggcgctgca atatttataa ttagtatccg tatacccttc catccatctt
      421 ctcaaccttc accctgaaaa ccataacctc tctcaccgcc ggttcgttgt attcaaaatc
      481 ccggtcagcg tattgcgcca tgatcctgtt caaacactct attttactgc cggcatcctc
      541 aataaattca gcctttccgt ataccaggac actcttgtag cgcatgctcc agctgcaggc
      601 cacttcttca ttaacatacc gtaactcatg atcggtgctg aaagcgatac acacatccgg
      661 gttaatcttc agtatatccg ctttcttccc ttttgccgag ccgtggaaat agacaaactc
      721 accgtcgtag ccgaaattca tcggaatgac atacggtttc ccatccggcg tggtcatggc
      781 cacatgacag aactggcatt tccggatgat cgcatcctgg tgctctttat tggttatctg
      841 ccggtttttc atattcgttt cgcctgacaa tcgtagttga acttgttccg aacaaggaag
      901 caacaatact caaaatgacg ctgaacagca gggcccacca gaacccgtcg accgtaaatc
      961 cgggaatgac cttcccgatg aacaggatga tcagcgcgtt gatcaccagc aggaagatcc
     1021 ccagggtgac gatggttacc gggatcgtca gaaataccag tatcggcctg acaaatgtgt
     1081 tcaggatagc cagcgaaatg gctaccatta cggccgtagt gaagttttca acactgactc
     1141 cgggcagcag ccaggcggtc agcaatacgg cgcatgtgta aactaagagt cggatcagta
     1201 ctttcatctt acaaagatat actttttact acctttgcat cctcaacttt caaaaccaaa
     1261 taagattaac gaaaacatgg aacatattca ttgcctgatc attgggtccg ggcctgccgg
     1321 atataccgca gctatttatg ccgccagggc agatcttcat cccgtcattt atgaaggaat
     1381 tcagccgggt ggacaattga caacaaccac ggaagtggaa aacttccccg gttatccgga
     1441 tgggatcatg ggtccgcaga tgatggaaga catgaaaaag caggccgccc gtttcggcac
     1501 cgatatccgt tttggcatcg tcactgaggt tgacctgtcg aaacggccat ttacagtgaa
     1561 agccgatgac ggcaagacga tgctggccga tacactgatc attgccaccg gagccacggc
     1621 caaatacctc ggcctggaat cggaagagcg gttcatgggg gcgggcgttt cggcctgtgc
     1681 tacctgcgac gggttcttct accgcggaaa agatgtggca attgtcggcg gcggcgatac
     1741 ggcagcggaa gaggcctcct acctggccaa actctgccgc accgtacacc tgatccaccg
     1801 ccgtgaagag ttgcgtgctt cgaaagccat gcagaagcga gtgaccacca cgccgaatat
     1861 cgtccttcac tggaattgtg tagtgaaaga aattgtcggc accgaagaag gttttaacaa
     1921 agcggtgacc ggcgtccgta ttgaaaatat caaaacaggt gagcaggaac tgctaccagt
     1981 cgatggttat ttttccgcta tcggccacaa accgaattcc gacctgttta aaggggtatt
     2041 ggatatgaat gaggtgggtt atctcattac caaacccgat tcaaccgaaa cgaatgttcc
     2101 gggtgttttt gcctgcggcg atgtccagga ctggaaattc cgccaggcgg tgacggcggc
     2161 agggagcggc tgtatggctg cccttgaggc cgaaaggttc ctcgcagcac aggaataaga
     2221 tggaaaaccg ggagaagcag atcgtccgtg cctcctgggt cagcatcgcc ggaaacgctc
     2281 tcctgtcggt gcttaaaatc tttatagggt tgatttccgg atccatggcc gttttggcgg
     2341 atgggatcga ttcggccggc gatatcatcg catcagctat caccctg
//
LOCUS       DAOQXS010000452         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_50149_length_2377_cov_3.287683, whole genome shotgun sequence.
ACCESSION   DAOQXS010000452 DAOQXS010000000
VERSION     DAOQXS010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50149_length_2377_cov_3.287683"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2242)
                     /locus_tag="PKM34_11015"
     CDS             complement(<1..2242)
                     /locus_tag="PKM34_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84166.1"
                     /translation="MSLIENPGLFSISGILVGTGAAWTLQLARDGMIYCGNMLDYPYY
                     SMNVISKPWISGPGCSFEMCNVSTYPGELEEQFNNILVDYLLRFEWTGEQCQGYPIQF
                     KPNFIPTPQTIQWDFDDGPGSNSWQLSPAYAFKNPGLHEVKVDVWYPSGRYEHTSREI
                     EISPSPQPDLGPDTLICNGASITLNANCEADFFSWSSGQFGVSSITVSDSGTYRVRCR
                     FNESGCMGYDTIHVGFHLPTLIDETNLVIIPTTCNGASGSITGLTALGSTPFAYQWRD
                     LSGNDYGVNIDASGLPAGQYYLTIIDGNGCETESPVYTIEDAGNLQVLNVELTQPHCG
                     RPDGQVFVHAFSPSGSTLEYSINDGADYSSDSIFTGLSGGIYVVHIRDVNGCEGFYLA
                     NPVILADIPGPQVAQVNITDETDFLGNGSVEIIATGSTSQIFYSIDNGGTWQTNNGTF
                     NDLSAGTYTCIVKDENDCDTSFTVDVQNIILTYLHAVTDEGEHCLGNTAMVPVNVDNF
                     NNVADFHLKLGYNADNLECEGFANVNTQLADSLTGWIDPIAGDIHLVWHSPAPVTFSG
                     TEKVADMVFTTRNPGQGELAWYTGETESYFTNASGNPIPAQFQTGEVTIYEPPMIILD
                     QSKTVCTGQFVSMMSIAVGNQPPLDYQWIYPDGDTTGNDPFFFAVTQTDAGIYTLLAT
                     DLVGCTDQKSIELIVSDNPVAAFHGTDTLEMQAGDVLDAGTGLSSYRWNTGDSTESIV
                     INAEGMY"
BASE COUNT          601 a          577 c          596 g          603 t
ORIGIN      
        1 agtacattcc ttcagcattg atgacaatac tttccgtgct gtcgcccgta ttccagcgat
       61 aggatgaaag tccggttcct gcatcaagca catcccctgc ttgcatttcc agcgtatccg
      121 ttccatggaa ggctgctacc ggattatcgc tgacgatcaa ctcaatgctt ttctggtcgg
      181 tacagccgac aagatcggtg gcgagtaagg tgtaaatgcc tgcgtcagtt tgggtaacgg
      241 cgaagaagaa cggatcgttg ccggtggtat caccatcggg gtagatccat tgataatcaa
      301 gggggggctg gttgccgaca gcaatgctca tcatgctgac gaactggccg gtgcagacgg
      361 ttttgctctg atccaggatg atcatgggcg gctcatagat cgtgacctcg cctgtctgga
      421 actgggcggg aataggattg ccactggcgt tggtgaaata cgattcggtt tctccggtgt
      481 accaggccag ttctccctgc ccgggatttc tcgtggtaaa caccatgtcg gcgacctttt
      541 ccgtcccgct gaatgtcacc ggtgccggtg aatgccagac cagatgtatg tctcctgcga
      601 tcggatcaat ccagccggtc aggctgtcgg caagctgggt attcacattg gcaaagcctt
      661 cacattccag gttatccgca ttatagccca gtttcaggtg aaaatcggcc acgttgttaa
      721 aattatcgac attaaccggg accatagcgg tattgcccag gcagtgttct ccttcatccg
      781 taacagcgtg taaataagtc aggataatat tttgaacatc aaccgtaaag gacgtatcgc
      841 agtcgttttc gtctttgacg atacaggtat aggtgcctgc agaaagatca ttaaatgtcc
      901 cgttattcgt ttgccaggtg cctccgttgt caatggaata gaagatttga gatgtgctac
      961 cggttgcaat aatttcaacc gagccgttcc ccaggaaatc tgtttcatcg gtaatgttaa
     1021 cctgtgccac ctgcggtccg ggaatatcgg ccaggataac cgggtttgca agataaaatc
     1081 cttcgcagcc attgacatcc ctgatatgga cgacgtaaat accgccagat aatccggtga
     1141 aaatgctatc cgatgaatag tccgctccat cattgataga atattcaagt gttgatcctg
     1201 aaggagaaaa agcatgcaca aagacctggc catccggcct tccacagtgt ggttgagtga
     1261 gttcaacatt taaaacctgc agattgccgg catcttcgat ggtgtaaacc ggagattccg
     1321 tttcgcaacc gttgccatca ataattgtca ggtaatattg ccctgcgggg aggcccgatg
     1381 catcgatgtt cacgccgtaa tcgtttcctg acaggtcgcg ccattgatat gcgaagggcg
     1441 tagagccaag agcagtgagt ccggttattg aaccgcttgc tccgttgcag gtggtgggga
     1501 ttataacaag attggtttcg tcgatgagag ttggtaggtg gaagcccaca tggatcgtgt
     1561 cataacccat gcagccggat tcgttgaaac gacatcgaac ccggtaagtt ccgctatcag
     1621 aaacggtaat tgacgaaacg ccgaattgtc cgctggacca ggaaaagaaa tcggcttcgc
     1681 aattagcatt gagcgtaatg gaagcgccgt tgcaaatgag tgtatccgga ccaagatcgg
     1741 gttgaggcga tggggagatt tctatctctc ttgaagtatg ctcatatctc ccggaaggat
     1801 accacacatc aaccttcact tcatgcaagc ctgggttttt aaaagcatag gccggtgaaa
     1861 gctgccatga gttgcttccc ggtccgtcat cgaaatccca ctggatagtt tgtggagttg
     1921 gaatgaagtt cggcttaaac tggattggat atccctggca ctgttcgccg gtccactcga
     1981 agcggagaag atagtccacg aggatgttat taaattgttc ctctaattca cccgggtatg
     2041 tgcttacgtt acacatctca aaggagcatc ccggcccgct tatccagggt ttgctgatca
     2101 cattcataga ataataagga tagtccagca tatttccgca ataaatcatt ccatcccttg
     2161 caagttgcag ggtccatgca gctcctgtgc caaccaggat gcccgaaatg ctgaacaagc
     2221 ccggattctc gatcaaactc atgggaaact gataaacatc aaccgaatcc caacccccaa
     2281 aagcaacgta cataaatttc gaatccggcg aaaattcaac tccccgtgga gcaattcgtc
     2341 cgttcatgct attcaatgaa tacatgtatt cagcgct
//
LOCUS       DAOQXS010000453         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_50460_length_2368_cov_3.443147, whole genome shotgun sequence.
ACCESSION   DAOQXS010000453 DAOQXS010000000
VERSION     DAOQXS010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50460_length_2368_cov_3.443147"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKM34_11020"
     CDS             <1..376
                     /locus_tag="PKM34_11020"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNQ84167.1"
                     /translation="TALFDKDNLKWKDLVDPGTPIPTPWNKEPFDRFGYRIQQERDSL
                     RAAKVPEELMEALFMRQMKQEDEFFAAEKYREQVGAFEGAGYLAKGLYRPQIDCIMYT
                     RHMEFCKVCRRSLEDVINMYSR"
     gene            382..1248
                     /locus_tag="PKM34_11025"
     CDS             382..1248
                     /locus_tag="PKM34_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ84168.1"
                     /translation="MKLTGFIFFRLFSGFIGLLPFPVLYSFSFFLSFILKRVIKYRKR
                     IILQNLTGCFPRMDERALNDTMGRFYLNLTDVMLEGLKAFTMSGRQVLARYRIRNAEV
                     IKPYVDAGRSVICVTGHYGNWEWGALSPPLQTRFRFIAFYKPLKYKRFDSYARNNRSR
                     FGTYLAPIRETTKTFEENRGNPAVYLMAADQNPSNREMAYWVDFLNRETAFLHGPEKH
                     ARINNYPVVFAAIRRTARGYYELELSVLADDPSVLPEGEITTRYAASMEKLIREDPAN
                     WLWSHNRWKYSR"
     gene            complement(1240..>2368)
                     /locus_tag="PKM34_11030"
     CDS             complement(1240..>2368)
                     /locus_tag="PKM34_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ84169.1"
                     /translation="SLCFLPLSHIFERTWTFYLMHCGAVNVFLENPREVIKVLPLVNP
                     TVMCTVPRFFEKTYDGIQVEKEKWPKIKQNIFNWAVATGLRCNEHKKSSSKIPVVLNF
                     KLKLADLLVLKKLRKVFGNKMRQMPCAGAAIREDLLRFFHAAGLFVNYGYGATETTAT
                     VSCFKKEEYSFQTCGTAMPGLDIKFTEEGEIMVKGPTVFRGYYRKPAETEMVLRDGWY
                     MTGDKGHMTKEGDLVMTDRLKDMFKTSVGKYVSPQKLELLLGQEKLIEQVIAVGDNRK
                     FVSALIVPSFDNLKTTAENLGIDINDRHKVVSHDTVQKIFRDKLEELQQELTPYERVV
                     KFTLLTEPFSIENNAMTSTLKLKRKVITEKHIAVIEEMYSS"
BASE COUNT          589 a          567 c          553 g          659 t
ORIGIN      
        1 taccgcgctc tttgataaag ataacctgaa atggaaagac ctggttgacc ccggtacccc
       61 cattcctact ccctggaata aagaaccgtt cgaccgtttt ggttacagga tccagcagga
      121 acgcgacagc ctgcgtgccg ccaaagtgcc cgaagaactg atggaagcgc tttttatgcg
      181 ccagatgaag caggaagacg agttcttcgc cgctgagaag taccgtgaac aggtcggggc
      241 cttcgagggt gccggctacc ttgccaaagg actgtaccgg ccacaaatcg attgtatcat
      301 gtacacacgg catatggaat tctgtaaagt ctgccggcga agcctggaag atgtgataaa
      361 tatgtattcc aggtagccag tatgaagctg accggattta ttttcttccg cttgttctcg
      421 ggatttatcg ggctgttgcc ttttcccgta ttgtactcat tctccttttt tttatccttc
      481 atcttaaaaa gggttattaa atataggaag agaataatac ttcaaaatct caccggttgc
      541 tttccccgaa tggatgaacg ggccctgaac gatacaatgg ggaggtttta cctaaacctg
      601 accgatgtta tgctggaagg gctcaaagct tttacaatga gcggacggca ggtacttgcc
      661 cggtatcgca tccggaatgc agaggttata aaaccttacg ttgatgccgg acggagtgtg
      721 atctgtgtca caggacatta cgggaactgg gaatggggcg ccctgtcacc acctctacag
      781 acgcgttttc gcttcatagc tttttataag ccattgaagt acaaaaggtt tgactcctat
      841 gccaggaata accggtcacg tttcggaaca tatcttgcac cgatccggga aacgaccaag
      901 acatttgagg aaaaccgggg caatccggcg gtttatctca tggcagccga ccagaatccg
      961 tcgaaccggg agatggctta ctgggtagat tttttgaaca gggaaaccgc ttttctgcat
     1021 ggcccggaaa aacatgcacg catcaataat tatccggtcg tatttgctgc tatccgtcgt
     1081 acagccagag gttattatga actggaattg tcggttctgg cagatgatcc ttctgtttta
     1141 ccggaagggg agatcacaac ccgttacgcc gcaagcatgg aaaagctgat ccgtgaagat
     1201 cctgccaatt ggttatggtc acataatcga tggaaatatt cacgatgaat acatttcttc
     1261 tatcactgcg atgtgctttt cagtgatcac tttccgttta agcttgagtg tactggtcat
     1321 tgcattgttc tcaatactga atggctcggt cagcaaagtg aacttaacta ctcgttcgta
     1381 aggggtcagc tcctgctgca gctcttccag tttatcccgg aatatttttt gaacggtatc
     1441 gtgggaaacg actttgtgcc tgtcgttgat gtcaatcccc aggttttctg cagtcgtttt
     1501 caggttatcg aatgatggga caatcagcgc cgaaacgaat ttccggttat cacccacagc
     1561 aattacctgt tcgatcagct tttcctgccc gagcaacagt tccagttttt gcggggacac
     1621 atatttgcca accgaagtct tgaacatatc cttcaaccgg tcagtcataa ccaggtcacc
     1681 ctcttttgtc atatgacctt tgtctccggt catataccag ccatctcgca ataccatttc
     1741 ggtctcggcc ggtttccgat aatacccgcg gaataccgtc ggccctttga ccatgatttc
     1801 gccttcttcg gtgaatttta tatccaggcc gggcattgcg gttccgcagg tctgaaaact
     1861 atattcctcc tttttaaaac aggataccgt tgcggtagtt tctgttgccc cgtaaccata
     1921 attgacaaat agtcctgcag catgaaagaa cctgaggaga tcttcacgga tggctgcgcc
     1981 ggcacacggc atctggcgca ttttgtttcc gaataccttc cggagtttct tcagtacaag
     2041 caggtcggca agttttaatt taaaattcag aaccaccgga atttttgatg aagatttttt
     2101 gtgctcgttg caccgcagcc ccgtcgctac agcccagtta aaaatgtttt gcttgatttt
     2161 gggccatttt tctttttcaa cctgtatccc gtcatacgtt ttttcgaaaa accttggcac
     2221 ggtgcacatg accgtaggat tgaccaacgg cagaactttg atgacttccc tggggttttc
     2281 aagaaaaaca ttcactgcac cgcaatgcat caggtaaaag gtccaggtcc gttcaaatat
     2341 atggcttaag ggcaggaaac aaagtgat
//
LOCUS       DAOQXS010000454         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_50781_length_2359_cov_3.193576, whole genome shotgun sequence.
ACCESSION   DAOQXS010000454 DAOQXS010000000
VERSION     DAOQXS010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50781_length_2359_cov_3.193576"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKM34_11035"
     CDS             <1..946
                     /locus_tag="PKM34_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNQ84170.1"
                     /translation="RGVPLAVQDDLRVFPESGDSQSIINCLLREALRLGIMIQTGMPV
                     REIRAEGEKLAIIFVGDENPARLFDRVIVAAGGSPQRRGLDWLEMMGHKIEDPVPSLF
                     TFNMPGEKIKDLMGVSATKALAKIQGTKFLAEGPLLITHWGMSGPAILKLSSFGAREL
                     SRMEYKFKLQVNWACEQNNEIVLAYLQEMASTHPQKMLVNLRPHDLPIRLWQYLLQKA
                     DLAENKPWAATGRKGLNKLVNILTNDVYEVDGRTTFRDEFVTCGGVSLESVDINTMQS
                     KVVKNLYFAGEVLDIDGITGGFNLQAAWTTGWIAGISC"
     gene            complement(1036..1590)
                     /locus_tag="PKM34_11040"
     CDS             complement(1036..1590)
                     /locus_tag="PKM34_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNQ84171.1"
                     /translation="MVPIAKNITSDDIVFLGDSLTEGFDLQHYFNLPNLKNRGISGDT
                     TYQVLYRLEEIWNSKPAGLFLMIGINDMFQGEDEPVILQNITLIISELQKHSPGTALF
                     VQSILPVNLSVMFADEFIDLSIFSFNNNLRLVCKELNVRYIDLYADFLDDAGELDRQY
                     TYDGVHLSPEGYDLWAKLIRPFLI"
     gene            complement(1639..1971)
                     /locus_tag="PKM34_11045"
     CDS             complement(1639..1971)
                     /locus_tag="PKM34_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84172.1"
                     /translation="MGLNKGKHIVGEVDGVRCTIVESGISKERAAFLKELLEFNGYEV
                     RIQEINADPPDDTKTFTLGVTDIVFNPVIAVYQKTLKRKDGKKVTPAFWNQTGIGEGL
                     PYWLVGRA"
     gene            complement(1984..>2359)
                     /locus_tag="PKM34_11050"
     CDS             complement(1984..>2359)
                     /locus_tag="PKM34_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ84173.1"
                     /translation="NMDDYNGLYRTNPKLSLVMMLALFSLAGIPPVAGFFGKFFLFTA
                     AAEQGFYYLVLIALLNTIVSLYYYLLIIKAMFLTKSDDAIAYFKSDRPTRLGLILCVA
                     GIVVVGFYSPIYEMIKSLSFGI"
BASE COUNT          629 a          556 c          576 g          598 t
ORIGIN      
        1 acgtggtgtt ccattggccg tacaggatga tttgcgcgtt tttcccgaat cgggtgattc
       61 acaaagcatt atcaattgtc tcttaagaga agcactacga ctcggtatta tgatccaaac
      121 cggaatgccg gtgagggaga tcagagcgga gggtgagaaa ctggcaatta tttttgtggg
      181 agacgaaaac ccagccaggc tttttgacag ggttatcgtg gcagccggag gctctcccca
      241 gcgcaggggg ctggattggc tcgaaatgat gggccacaaa atcgaagatc ccgttccgtc
      301 gttgtttact ttcaatatgc ccggggaaaa gatcaaagac ctgatgggcg tttcagccac
      361 taaggctctg gcaaaaatcc aggggacaaa gtttcttgct gaaggacctt tgctgatcac
      421 gcactggggc atgagcggac cggctatttt gaaattgtca tcctttggcg cccgggaatt
      481 gagccggatg gagtataaat tcaaattgca ggtcaattgg gcctgtgaac aaaacaatga
      541 aatagtcctt gcttatctgc aggagatggc tagtacccac ccccaaaaaa tgctggtaaa
      601 tctccggccg catgatctgc cgatacgctt atggcaatat ttacttcaaa aagccgacct
      661 tgctgagaat aaaccctggg ctgcgaccgg ccgcaaaggg ctcaataaac tggtcaatat
      721 cctgaccaat gatgtttatg aagtggatgg ccggactacg ttcagggatg agtttgtgac
      781 ctgcggcggc gttagcctgg agagtgtcga tataaacacg atgcaaagca aagtcgttaa
      841 aaatctctat ttcgccggag aagtacttga tatcgatgga attaccggtg gttttaatct
      901 ccaggccgcc tggacgaccg gttggattgc aggaatatcc tgctgagttc ataccaaagt
      961 cattcgtata atccgtgtca tcagtgttcc attatctgaa cacaaataca atgaattata
     1021 caaatttata cagggttaaa tcaggaatgg tctgatcagc ttagcccaga ggtcatatcc
     1081 ttccggtgaa agatgtaccc cgtcataggt atattgccgg tccaattccc cggcatcatc
     1141 caggaagtcg gcgtacagat caatatagcg gacgttaagt tccttgcaaa ccagccttaa
     1201 attgttatta aaactgaaga ttgacagatc gatgaactca tcagcaaaca taaccgaaag
     1261 gtttaccggc agaatgcttt gtacaaacag tgccgttccc ggtgaatgtt tctgcagttc
     1321 actaataatc aacgtaatat tttgaaggat gaccggttca tcctcaccct ggaacatatc
     1381 gttgatgccg atcatgagaa aaagcccggc aggtttggag ttccatatct cctccaggcg
     1441 atagagcacc tggtaggtcg tgtcgccact gataccccgg ttcttcaggt taggaagatt
     1501 gaaatagtgt tgaaggtcaa agccttccgt caggctgtcg cccagaaaaa cgatgtcatc
     1561 ggatgttatg ttctttgcta tcgggaccac cttgaacctg gaatttggaa cctggaaact
     1621 ggaacctgga acctggaact atgctctccc aaccagccag taaggcagcc cttccccgat
     1681 cccggtctgg ttccagaaag cgggggtaac cttttttccg tctttccgct tcaacgtttt
     1741 ctggtaaacc gcaatgaccg gattgaagac gatatcggtt accccgagag tgaatgtttt
     1801 cgtgtcgtct ggcggatcgg cgttgatttc ctggattctc acttcataac cgttgaactc
     1861 cagcaactct ttcagaaaag cggccctctc ttttgatatc cccgattcaa ctatagtgca
     1921 gcggactccg tccacttcac cgacaatgtg ttttccttta ttcaatccca ttattctgtc
     1981 aaattaaatc ccgaaactca ggctttttat catttcatag atcgggctgt aaaatcccac
     2041 aacgacgatg ccggccacgc aaaggatcaa tcctaaccgg gtgggccggt cgcttttgaa
     2101 ataagcaatg gcgtcgtcgc ttttcgtcag gaacatagct ttgatgatca gcagatagta
     2161 gtagagcgaa acgatcgtat tcagcagagc gatcaggacc aggtagtaga atccctgttc
     2221 tgccgcggcg gtaaacagga agaactttcc gaagaacccg gcaaccggag gaatacccgc
     2281 aagggagaac agcgccagca tcataaccag gctcagtttc ggattggtgc ggtacaaacc
     2341 gttgtagtca tccatattc
//
LOCUS       DAOQXS010000455         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_50919_length_2355_cov_3.148696, whole genome shotgun sequence.
ACCESSION   DAOQXS010000455 DAOQXS010000000
VERSION     DAOQXS010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50919_length_2355_cov_3.148696"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1571
                     /gene="glyA"
                     /locus_tag="PKM34_11055"
     CDS             291..1571
                     /gene="glyA"
                     /locus_tag="PKM34_11055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ84174.1"
                     /translation="MKRDYQVFRILGKEKKRQLHGIELIASENFVSNQVLRAMGSVMT
                     NKYAEGYPGKRYYGGCQFVDETEQLAIDRAKELFNCEYANVQPHSGAQANMAVFLAFL
                     KPGDKFMGLDLSHGGHLSHGSPVNSSGILYQPIAYGVDKETGRVDYDKMEEIALREQP
                     KLIIAGASAYSRDWDYARMRAIADKAGAILMADISHPAGLIARGLLNDPMPYCHVVTT
                     TTHKTLRGPRGGLILMGKDSENPWGWATPKGEIKMMSQLLNSAVFPGIQGGPLEHVIA
                     SKAVAFGEALTDEYFEYILQVRKNASVMAKAFVSKGYHVISGGTDNHLMLIDLRNKFP
                     ELTGRLVENTLVRADITVNKNMVPFDSRSPFQTSGLRIGTPAVTTRGLKEKEMPVIVD
                     MIDEVLHNIENETILLSVKKRVNEMMEKYPLFSA"
     gene            1785..>2355
                     /locus_tag="PKM34_11060"
     CDS             1785..>2355
                     /locus_tag="PKM34_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84175.1"
                     /translation="MITPSFIPKSVSVLAIVIILLGSSAAFSQYPVVTVRFNNPEYVC
                     ATQTYSLDVEMQCDSANKRLHGMNIRFFYDDDVLEFTSFTEIIAAYGLTIPNPPEITT
                     DSTGLSMELFGIEGNYEYVNGAVKVVSSTPVTYLPTNGWTKIFAVNFHVDDPEVFQDS
                     TFCPAVIWDLQEDPSLGGMMGGGGVCITLV"
BASE COUNT          634 a          573 c          588 g          560 t
ORIGIN      
        1 tagttgctga atgaggcccc cagttgcagc ggttgccagc gtacctgcac atcacctccg
       61 ggcagcacct ccaggcattt cagattcact gcctgcccgg tgagagcagc cggaagcata
      121 acgaggataa ggaaaatcat gatgaaagca ggcttgcgca tccggttgaa atgtcttgca
      181 aaattagtca aaaatcaggg gcaggtaaac ggaatcccat taacgatctt catatttcca
      241 ttacctttgt aaccgttttt tgaaaattaa ccataacaaa caatgtgtat atgaaacggg
      301 attatcaggt atttcgtatc ctgggtaagg aaaagaaaag gcagcttcac gggattgagc
      361 tgatcgcatc ggagaatttt gtcagcaacc aggtcttgcg ggccatggga tcggtgatga
      421 ccaataaata tgcggaaggc tatccgggca agcgttatta tggcgggtgc cagtttgtgg
      481 acgaaacgga acaactggcg atagaccgtg ccaaagagct gttcaattgc gaatacgcca
      541 atgtgcagcc ccattccgga gcccaggcca atatggcggt tttcctggcc ttcctgaaac
      601 ccggcgataa attcatgggg cttgacctgt cgcatggcgg gcatctctct cacggatcac
      661 cggtgaacag ttccggtatc ctctatcagc cgatcgctta cggtgtggat aaagagaccg
      721 gcagagtgga ttatgacaag atggaagaaa ttgccctgcg ggagcaaccg aagctgatca
      781 ttgcaggggc atcggcttat agcagggact gggactatgc caggatgcgg gcgatagccg
      841 acaaggccgg cgccatcctg atggccgata tttcgcaccc ggccggcttg atcgccaggg
      901 gattactgaa cgatccgatg ccgtattgcc atgtcgttac caccacaacc cacaaaaccc
      961 ttcgcggacc acgcggcggg ctgatcctga tgggcaaaga cagcgagaat ccttggggat
     1021 gggcgactcc gaaaggggag atcaaaatga tgtcgcaact gctgaattct gccgttttcc
     1081 cgggaatcca gggcggcccg ctggaacatg tcatcgcttc caaagccgtt gccttcggcg
     1141 aagccctcac cgatgaatat ttcgaatata tcctgcaggt gcggaaaaac gcctccgtca
     1201 tggcaaaagc ctttgtcagc aaaggctacc atgttatttc cggaggaacc gacaatcacc
     1261 tgatgctgat cgatctgcgg aataaattcc ccgaactcac cggccggctg gtcgaaaata
     1321 cactggtaag ggcggatatt accgtcaaca aaaacatggt tccgttcgac agccgttccc
     1381 cgttccagac ctccgggctg cggatcggga cgccggctgt aaccacccgc ggactgaagg
     1441 aaaaagagat gccggtgata gtggatatga tcgatgaagt actccacaac atcgaaaatg
     1501 aaacgatcct gctctccgtc aaaaaacggg tcaatgaaat gatggaaaaa tatccgttat
     1561 tttctgcctg aaactgaaac ttactctctg attttcagta taatcttttc aaaatccttc
     1621 ctggaatctt cagcttagcc aggacttaac cggaatgaaa ccgcattatc aggaatcatt
     1681 ctaaattgca acaaattaag tcaattgcta tcctgaaaat ataaccttga atggattgaa
     1741 ggattaatgt atgtatttat atttgttacc gaaaatcaag agacatgatc acaccatctt
     1801 ttatccctaa aagtgtctcc gtgctggcca tagttattat tctgcttggc tcttcagcgg
     1861 cgttttcaca gtaccctgtc gttacggttc ggtttaataa cccggaatat gtatgcgcta
     1921 cccagactta ttcactcgat gtagagatgc aatgtgactc ggccaataaa cggctgcacg
     1981 gaatgaatat ccgttttttc tatgacgacg atgtcctgga gttcacatct ttcactgaaa
     2041 tcattgccgc atacgggctt acgattccca atcccccgga aataaccacc gacagcacgg
     2101 gattaagcat ggagttattc ggcatagaag gcaattacga atatgtgaac ggggccgtca
     2161 aagttgtatc cagcacaccg gtcacttact tgccgaccaa cggatggaca aaaatctttg
     2221 cggtgaattt tcatgtcgat gatccggaag tattccagga ttcaactttc tgccctgctg
     2281 tgatctggga tctccaggaa gacccttccc tgggaggcat gatgggcgga ggaggggttt
     2341 gcatcacact ggtaa
//
LOCUS       DAOQXS010000456         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_51374_length_2341_cov_3.143920, whole genome shotgun sequence.
ACCESSION   DAOQXS010000456 DAOQXS010000000
VERSION     DAOQXS010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51374_length_2341_cov_3.143920"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKM34_11065"
     CDS             complement(<1..1080)
                     /locus_tag="PKM34_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNQ84176.1"
                     /translation="MKVKYLDLIEQTFDFPTNEFEVIDYELNFHEIPLMDIIKQYGTP
                     LKISYLPKISEQIQKAKRWFNVAMAKADYNGNYIYCYCTKSSHFSFILQEAMKNDIHI
                     ETSSAFDIHIVEQLFEEGRINKDTHIICNGFKRPQYIENIINLINRGFENTIPVLDNI
                     FELDKFSKGIRKKVKLGIRIASEEEPKFEFYTSRLGIRYNDIVSYYQEKIQNNSKFAL
                     KMLHFFINTGIRDTAYYWNELSKCVNVYCELKKICPELDSLNIGGGFPVKNSLTFDYD
                     YEYMAEEIISQIKIICNQNNVPEPDIFTEFGSFTVGESGAVLYSIIDQKRQNDREIWN
                     MIDSSFMTTLPDTWALNQRFILLAVN"
     gene            1399..>2341
                     /locus_tag="PKM34_11070"
     CDS             1399..>2341
                     /locus_tag="PKM34_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNQ84177.1"
                     /translation="MQSKGAIKVFAIAFALVCLFQLSFTFFSGITERKARKYSTNAEA
                     MRLADELAGGSQRSKTFVMDSLAKARESYYLDSMANQPIYNILVKEYTYKEVKEREIN
                     LGLDLRGGMNVVLEVSIGEIVKALAGPNAVNPIFQQAMERAYEKQKSSDRDFVTLFGE
                     AFREVDPNANMASIFSTVEFKDKITYNSTNDEVLEVLRDETEGAFDMTYNIIRTRIDR
                     FGVTQPNVQKLTGTGRILVELPGIKEPARVRKLLQGTAKLEFWETYNYSEVYGYFADA
                     NSRLVTILGSQNLEDNGTAGKTTEGETSDTGENGSSAT"
BASE COUNT          707 a          471 c          469 g          694 t
ORIGIN      
        1 attgacggct aaaaggatga acctttggtt tagcgcccag gtatccggaa gtgttgtcat
       61 aaaagagctg tcgatcatat tccagatctc ccggtcgttc tgtcttttct gatcaatgat
      121 ggaatagagc actgccccgc tttcaccgac cgtgaaggaa ccgaattcgg taaatatatc
      181 gggttcaggc acattattct gattgcaaat gattttaatc tgtgaaatga tctcctctgc
      241 catgtactcg taatcataat caaatgtaag agagttcttt acgggaaatc caccgccgat
      301 gttcagcgaa tcaagttcgg ggcatatttt tttgagctca cagtaaacat tgacgcattt
      361 cgacagctca ttccagtaat aggctgtatc ccggataccg gtattgatga agaagtgcag
      421 cattttcaag gcaaatttgc tgttattctg tattttttcc tggtaatagg aaacaatatc
      481 attatacctt ataccaagcc tggaggtata aaattcaaat ttcggttctt cttccgaagc
      541 aatcctgatc cctaatttta ccttttttct aatgcctttt gaaaatttat ccagctcgaa
      601 aatattatcg aggaccggaa tagtattttc aaagccacgg tttatcagat taatgatgtt
      661 ttcaatgtat tgagggcgtt taaatccatt gcatattata tgggtatcct tattgatccg
      721 gccttcttca aacaactgct ccacaatatg aatatcaaag gctgaagagg tttcgatatg
      781 aatatcgttt ttcattgctt cctgcaggat aaatgaaaag tgagaacttt tcgtgcagta
      841 acaatatatg taattgccgt tataatcggc tttggccatg gccacgttaa accatctttt
      901 agccttctgg atctgctcac ttatctttgg cagataactg atttttaacg gagtgccata
      961 ttgttttata atgtccatca gcggaatttc atgaaaattc agctcataat caatcacttc
     1021 gaactcattc gttggaaaat cgaaggtttg ttcaatcaga tccagatact taactttcat
     1081 atcaatacaa ttaacttgtt aaacggcagg caaagataat atattaaaga tatcactgtc
     1141 aagaaacttc aatccaactc acctaaatca ttaattttta ttcaatccat cttagtcgga
     1201 tttttaatta tctgatattc ttatgtttat acgtaataga aatgatacaa tttgtccttc
     1261 caattttcaa ttttcaaatt tccagttgta ttacattctt ttaccaataa aaaatgaagg
     1321 tactaatgga aattagaaat aatatgctaa ttttgcaccc tccaaaaaac aggtataaaa
     1381 tcataaaatt ctgtaaacat gcaaagtaag ggagctatta aggtatttgc gattgcattt
     1441 gcattagttt gtcttttcca gctatcattt acatttttca gcggcattac ggagcggaaa
     1501 gcgcgtaaat attccacaaa cgctgaggcg atgagactgg ccgatgaatt ggcgggcggc
     1561 agtcagagga gtaaaacctt tgtgatggat tcgctggcaa aagcgcgaga atcgtattat
     1621 ctcgattcca tggcgaatca acccatttac aatattctcg ttaaagaata tacttataaa
     1681 gaagtcaaag aaagggaaat caacctgggg ttggatctgc gaggtggtat gaatgtcgtc
     1741 ctggaagttt cgataggaga aattgtcaag gcactggccg gacccaatgc tgtcaacccg
     1801 atcttccagc aagccatgga acgggcctat gaaaagcaaa aatcgagcga tcgcgatttt
     1861 gtaaccttat tcggagaagc attccgtgaa gtcgatccca acgccaacat ggcttctatc
     1921 ttcagcacgg ttgaatttaa ggataaaatc acctataact ccactaatga tgaagttctt
     1981 gaggttttaa gagatgaaac cgaaggggcc tttgatatga cgtacaatat tatccgtact
     2041 cgtatcgaca ggttcggcgt tacccagccg aatgtccaga aactgaccgg aaccggccga
     2101 atcctggttg agctcccggg cataaaagag cctgcccgtg ttcgtaaact tctccagggg
     2161 acagccaagc ttgaattctg ggagacctac aactactcag aagtatacgg atattttgca
     2221 gatgccaaca gccgtcttgt gacgatcctc ggatctcaaa acctggaaga caatggcacg
     2281 gctggcaaga ccacggaagg agaaacttcg gataccggag aaaacggtag ctccgctact
     2341 g
//
LOCUS       DAOQXS010000457         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_52038_length_2322_cov_3.658139, whole genome shotgun sequence.
ACCESSION   DAOQXS010000457 DAOQXS010000000
VERSION     DAOQXS010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52038_length_2322_cov_3.658139"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..608)
                     /locus_tag="PKM34_11075"
     CDS             complement(66..608)
                     /locus_tag="PKM34_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNQ84178.1"
                     /translation="MRILREQSAGLVIDIQEKLFPFISGNDALTSSSVILVKGLQALK
                     IPIITTVQYTKGLGPTIRPLRELLPDEETIEKLSFSCCDDPVFMKKLESIQKRFIIIS
                     GIESHVCVLQTVTDLLEKGYVPVVAEDCVSSRKLNDKNMAIARMHRQGAIITTYESVL
                     FELLRYSGTEEFKAISRLVK"
     gene            complement(605..>2322)
                     /locus_tag="PKM34_11080"
     CDS             complement(605..>2322)
                     /locus_tag="PKM34_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84179.1"
                     /translation="AREIAAGYKSTDRFMLTVNEFEGRHQRWVNRDEFLQMIGEIVPT
                     PAVRILSEVIRRQQNSLTGSHGESVTSYLISDFQTSMADLENVKPDSLNPVFLVPLQA
                     VRTENLYIDSCWFVSPVHQIDQGVKLVARVVNDSRTDYEKVPLKLSVNGLQKAVASFD
                     ITAGTNYDVEIPFTNHEPGIQLGIVEITDYPVVFDDQMFIVYNVAGSIPVLAINGNQE
                     SPFLNSLFGKDPEFGFVNNPVGNIDYNRLREFELIILNEVENLSTGLIQELTIYLENG
                     GSLLIIPSEKLDIGSYTDFLQSVNVNFYGEFTRELTRVTEIDRDNPVFADVFERNVAS
                     ERGGNTMDLPEVRSFFPVTRNARTAQVPVMTMQNGKPFLTMEKAGKGLVYLLAVPLAE
                     SSGNFARHAIFVPALYRIALLSAASDPLYFVIGRDNRVSMSNPMLSGDYVLRIVSSGG
                     DYEFIPGMQTQNNLFNILINNQIRSAGHYFVRKGEENIKGLGFNYDRNESVMEFSGAE
                     ELNRMIDKYGFSRMRVISEDGKPLAESVKDMNQGTVLWRLFIILALVFLAFEIVLLRY
                     WKLKS"
BASE COUNT          595 a          584 c          543 g          600 t
ORIGIN      
        1 tcccggctct tcccgtcctt cggtcatatc ttccctgatc tcatccatcc ctgtattaac
       61 aaatattatt taaccagcct ggaaatggct ttaaactctt cggttccgga ataacggagc
      121 agctcaaaca gcaccgattc ataagtggta atgatagccc cctggcgatg catccgggca
      181 atcgccatat ttttatcatt gagtttacgg gaagaaacac agtcctccgc cactaccggc
      241 acgtaccctt tttccagcag gtcggtcacc gtttgcaaaa cgcagacatg agattctatg
      301 ccgctgataa tgatgaatct tttttgtatc gattccagtt tcttcataaa aaccgggtcg
      361 tcgcagcaac tgaacgagag tttttcaatc gtctcttcat cgggcaacaa ctcccggagg
      421 ggccggatcg tagggccaag ccctttggta tactgaacgg tggttattat gggtattttc
      481 agcgcctgca atcctttgac aaggataaca gagctgctgg tcagggcatc atttcccgaa
      541 ataaaaggaa ataatttctc ctgaatatcg ataaccagcc cggcagactg ttcgcgtaaa
      601 atcctcatga ttttaatttc cagtatctta ataaaacaat ttcaaaagcc agaaaaacca
      661 gggctaaaat aataaataac ctccacagga cagtaccctg gttcatatct ttcaccgatt
      721 ccgccaaagg tttcccatct tccgaaatga cgcgcatccg ggaaaatcca tatttgtcaa
      781 tcattcggtt cagttcctcc gctccggaaa attccataac cgactcattt cggtcatagt
      841 tgaatcccag ccctttgata ttctcttcgc cttttctgac gaaataatgg ccagccgacc
      901 gtatctgatt attgatcaaa atattaaaaa gattattctg agtttgcatt cccgggatga
      961 actcatagtc gccgccggat gaaacaatcc tgagcacata gtcgccgctg agcatgggat
     1021 tggacatgga tacccggtta tcccttccga tcacaaagta tagcggatcg ctggcggcac
     1081 taaggagtgc gatccggtat aatgccggta caaagatggc atggcgggca aaattaccgg
     1141 aggattccgc cagtgggaca gccagcaggt aaaccaggcc ttttcccgct ttttccatag
     1201 tcaggaatgg tttgccattc tgcatggtca taaccggaac ctgtgctgtg cgggcattgc
     1261 gggtaaccgg gaaaaagctc ctgacctcag gaaggtccat cgtgttcccg cctctttcag
     1321 aagcgacatt ccgctcaaaa acatcggcaa aaacagggtt atcgcggtcg atctcagtca
     1381 cgcgcgtgag ttcacgggta aattcaccgt aaaaattcac gttaactgac tgtagaaagt
     1441 cagtatagga tccaatatcc agtttttcag acgggatgat aagcaaggag cctccattct
     1501 caaggtaaat tgtcagttcc tggatcaatc cggtagaaag gttttcaact tcgttcagga
     1561 taatgagttc aaattcacga agccggttgt aatcgatatt tcccaccggg ttattgacaa
     1621 aaccgaattc gggatctttg ccgaacaaag agttcaggaa agggctctcc tggttgccgt
     1681 tgatggccag caccgggatg gaaccggcaa cgttgtacac aatgaacatc tggtcgtcaa
     1741 atacaaccgg gtaatcggtt atttcgacta ttcccagctg gattccgggt tcatggttgg
     1801 taaacgggat ttcgacgtcg taattggtac cggcggtaat atcaaagctg gccacagcct
     1861 tctggaggcc gttcacggat agtttcagcg gaaccttctc gtaatcggtc cgggaatcgt
     1921 tgaccactct tgccacgagt tttactccct ggtcgatctg gtggaccggc gagacgaacc
     1981 agcaactgtc gatatacagg ttttcagtcc ggacggcttg caggggaacc aggaacacgg
     2041 ggttcagcga atccggttta acattctcca ggtctgccat cgaggtttga aagtcggaga
     2101 tcaggtagct ggtcacggat tctccgtgtg atccggtaag actgttttgc tgccgcctga
     2161 tcacttccga gaggatcctg acagcgggtg tcggtacgat ctccccgatc atctgcagaa
     2221 attcgtcacg gttcacccat cgttggtgac ggccttcaaa ttcgttcacc gtgagcataa
     2281 accggtcggt cgatttataa cccgctgcaa tttcacgcgc cg
//
LOCUS       DAOQXS010000458         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_52412_length_2311_cov_3.903812, whole genome shotgun sequence.
ACCESSION   DAOQXS010000458 DAOQXS010000000
VERSION     DAOQXS010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52412_length_2311_cov_3.903812"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1304)
                     /locus_tag="PKM34_11085"
     CDS             complement(24..1304)
                     /locus_tag="PKM34_11085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNQ84180.1"
                     /translation="MARFEILMPKMGESIIEATITKWLKKAGDPIAEDEAIAEIATDK
                     VDSEIPSPVDGVVEKVLFNEGDVVPVGKVIAVINLGGDEETGEKEDKETESQGDKETA
                     GKEDKETVKKGDGRFYSPLVKNIAKQENIPLSELDKISGSGKEGRITKDDILNYAQLR
                     KQGAPVPEVSAHPPTIKTPQAVEVPVVTTEAGDEVFEMDRMRRLIADHMVMSKQVSPH
                     VTSFIDVDVSRIVTWREKVKDDILARELEKITYTHILMETCARVLKEFPRVNASVDGY
                     KIIHRRRINIGMATALPNGNLIVPVIKDADQKSLLGIIKNVNDLANRARKNSLLPDEI
                     QGGTFTITNFGTFDSLSGTPVINQPQVAILGTGAIRKKPVVVESEYGDIIAIRHIMIL
                     SLAYDHRVVDGALGGMFLKRMKELLENWDMSRKF"
     gene            complement(1401..>2311)
                     /locus_tag="PKM34_11090"
     CDS             complement(1401..>2311)
                     /locus_tag="PKM34_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84181.1"
                     /translation="IFRGISVGTVIILVAYALLPENLRFSRAIILMGSVWAVIVMVIS
                     RTVCQFASDRTLSFTPQKNRRFVIVGDRHEAGRVRDLLHSSYGTPGFIGLVSANPMAT
                     RNEDFIGSLVQIKEIIRVYRIDEVIFCSKSMTHQSIIDQMSALQDTGVDYKIAPEDSL
                     SIIGSNSINTAGDLYTVNINAITNTENRRNKRLLDLAISLVAIILLPFLLFLVHQPFG
                     MVRNIFLVVVSRRSWVGYIHDDHYDIERLPHIRKGILHPLDAFGKDHISDEIRQRLNL
                     IYARDYSTATDLNIVFKGIRNLGRKS"
BASE COUNT          520 a          594 c          543 g          654 t
ORIGIN      
        1 tccattaata taaaatttaa aatttaaaac ttcctgctca tatcccagtt ttccagcagt
       61 tccttcattc gtttgaggaa cattccgccc agggcgccgt cgacgaccct gtggtcataa
      121 gcgagcgaca ggatcatgat atgacggatg gcaatgatat cgccgtattc cgattccacg
      181 acaaccggtt tcttccggat agcgccggtc ccgagtatgg ccacctgggg ctggttgatc
      241 accggggtgc cgctcaggct gtcgaatgtg ccgaaattgg tgattgtaaa tgtgcctccc
      301 tggatctcat ccggcaggag gctgttcttt ctggcccggt tggccaggtc attcacattc
      361 ttgatgattc ccagaaggct cttctggtcg gcgtctttga tcaccggtac gatcaggttg
      421 ccgttgggca gagctgtcgc catgccaata ttgattcgcc tgcgatggat gatcttataa
      481 ccgtcgactg aggcgtttac tctggggaat tctttgagca ccctggcaca tgtttccatc
      541 aggatgtgcg tgtaggtgat cttttcgagt tccctggcca ggatatcgtc tttaaccttt
      601 tccctccagg taactatccg gctcacgtca acatcgataa aagaggtgac atgcggtgaa
      661 acctgtttcg acatcaccat atggtcggct atcagccttc tcatacggtc catctcgaaa
      721 acctcatccc cggcctcggt ggtcactaca ggcacttcaa cggcctgcgg ggttttaatt
      781 gttggcggat gggctgatac ctccggtacc ggagctcctt gcttgcgtaa ttgagcatag
      841 ttcagaatgt catcttttgt tatcctgcct tcctttccgc tccccgaaat tttgtcaagc
      901 tcacttaatg gaatattctc ctgcttggcg atatttttca ccaatggaga ataaaacctc
      961 ccatctcctt ttttcaccgt ctccttgtct tcctttcctg ctgtctcctt atcgccctga
     1021 ctctctgtct ccttatcttc cttttccccc gtttcctcat ctccacctaa attaataacg
     1081 gcgatcactt tacccaccgg gacgacatcg ccttcattga ataaaacttt ttcaaccaca
     1141 ccgtctaccg gtgaaggtat ttcggagtcg accttgtctg tcgcaatttc agcaatcgct
     1201 tcatcttctg cgatcgggtc gcctgccttt ttgagccatt ttgtaatggt tgcttctatg
     1261 atgctttcgc ccatcttggg catcaggatt tcaaatctgg ccattgagtt tatgtttaat
     1321 gatatgctgc agcagattct acagcagtct gcaaagttaa caattaataa caaagtgatg
     1381 ggcggattgt tcgggccggt tcagcttttc ctgcccagat tcctgattcc tttaaagaca
     1441 atgttcagat cggtggccgt actgtagtcc ctggcataga tcagatttag cctttgcctg
     1501 atctcatcgg aaatatgatc ttttccgaat gcatccaaag gatggagaat tcctttgcgg
     1561 atatgcggca accgctcgat atcgtaatgg tcgtcatgaa tatagcctac ccaggaacgc
     1621 cggcttacca caaccaggaa tatattccgg accatgccga acggttggtg aacaaggaaa
     1681 aggagaaagg ggagcagaat aatggcaacc aggctgatgg ccaaatccag taaccgcttg
     1741 ttccggcggt tttctgtgtt ggtaatggca ttgatgttaa cggtgtaaag atctccggcg
     1801 gtgttgatgg agttgctgcc gataatagag aggctgtctt ccggggcaat tttgtaatcc
     1861 acgccggtat cctgcaatgc cgacatctgg tcaattattg actggtgggt catgcttttg
     1921 gaacaaaaga tgacttcatc gatcctgtaa actctgataa tctccttgat ttgcaccagc
     1981 gaaccgatga aatcttcatt tcttgttgcc atggggttag cggataccag cccgataaat
     2041 cccggcgttc cgtacgaact gtgcagaaga tccctgaccc tgccggcttc atggcgatcg
     2101 cccactatga caaaccgcct gttcttttgt ggtgtgaagg agagggtccg gtcggaggcg
     2161 aattgacaga ccgtgcggct tatcaccata acgatgaccg cccataccga acccattaaa
     2221 atgatagccc tggagaaacg aaggttctca ggaagaaggg cataggccac caggatgatc
     2281 actgtaccga ccgaaatgcc gcgaaatatc c
//
LOCUS       DAOQXS010000459         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_52752_length_2301_cov_3.642030, whole genome shotgun sequence.
ACCESSION   DAOQXS010000459 DAOQXS010000000
VERSION     DAOQXS010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52752_length_2301_cov_3.642030"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PKM34_11095"
     CDS             complement(<1..948)
                     /locus_tag="PKM34_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84182.1"
                     /translation="MTGTAASQTTLELNKEKERYAFGQIYTGFRYGFKDTYKPQAAFE
                     FNQGIIGYFHQIAPKVSGKIMFDVTRTTNITSIKDSSGQSMTVTYFEGSKYTAYLKMA
                     EIKWDINDYIALRVGQLLNTQYLTFQDPFWGYRYIDVTFQERYRLGQPADFGAQLDVK
                     VKDKFLNQFSVVNGEGPFRHQDLNGQFIFSNNIQYTPVKNLTLKLYADYGPAVDTGSV
                     YADKWVISGFAGYKTQKFRFGGEYDFVNNYGWMNGKDYSGFSVFGGWNFTGKFDILVR
                     WDHLKINAAGVEDILNYYIAGIQYEAARMFTTSVNFRYQS"
     gene            1147..>2301
                     /locus_tag="PKM34_11100"
     CDS             1147..>2301
                     /locus_tag="PKM34_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84183.1"
                     /translation="MKKHYIFTCFIAIFLTLPLLNLYAQKPGIYFEPSSLAETLAPDD
                     SVVVHSVLHNGSAQTVEFSFPGYVTRDLGGPDDFGYSWIDSEEPGGPDWSWTDISEVG
                     ILVEGLGDDEMAGPFEMGFNFSFYGQTKNHFWISPNGVISFNEQFIPYANEPIPTNDN
                     YNDFIAWFWDDLKIDSAITRVYYKNYEEKTVVQFTKMVHYPGTESFITGQVIMMVNGS
                     ILLKYRLVSEDFETTGATAGIQSWNSQQGLQVVYNAEYVHSELAVRFDLNRNFITSVN
                     PSTLTLPPGTQETIWITYSSTGFEVGSYEQDLKCITSHPELPHLILHNVMHVNVPVNS
                     GFKGYVTDAATGFAIHEVMVKAGEQVTYTNDNGYYELPLEEGVYNVKFQRN"
BASE COUNT          629 a          549 c          508 g          615 t
ORIGIN      
        1 tgactggtag cggaaattaa cagaggtcgt gaacatcctg gccgcttcat attgaatccc
       61 tgcaatataa taattcagaa tatcttccac tcctgcagcg ttgatcttca gatggtccca
      121 ccggaccagt atatcaaact ttccggtaaa attccaccct ccgaaaacgg aaaaaccgct
      181 gtaatccttc ccattcatcc atccgtagtt attcacaaaa tcatattcgc caccgaaacg
      241 gaacttttgc gttttatagc ccgcaaatcc cgatatgacc catttatcgg cataaacaga
      301 gcctgtatcg acggcggggc cataatcggc ataaagcttc agggtaaggt ttttgacagg
      361 agtatactgg atattgtttg aaaagatgaa ttgcccgttg agatcctggt ggcggaatgg
      421 gccttccccg ttgaccaccg aaaactggtt caggaatttg tcttttacct ttacatccaa
      481 ctgtgccccg aaatcggccg gctgaccaag cctgtagcgc tcctggaaag taacgtcgat
      541 atacctgtat ccccagaaag gatcctggaa cgtgaggtac tgtgtgttca aaagctggcc
      601 gacgcgcaaa gcaatataat cattgatatc ccacttaatc tccgccattt tcaggtatgc
      661 cgtgtattta cttccctcaa aataggtaac cgtcatggat tggccggagg aatccttaat
      721 actcgtgata ttggtcgtgc gggtaacatc gaacatgatc ttcccgctga ccttcggggc
      781 gatctggtga aagtacccga taatcccctg gttgaattca aatgccgcct gcggcttgta
      841 agtatcctta aatccatacc tgaacccggt atagatttgt ccgaaagcat agcgttcctt
      901 ttccttgttg agttccaggg tggtttggct ggcagccgtt cctgtcatga aggataaaat
      961 cagaaaaaag ataatattgc gcataagaat cagaatgaca aaggcaaaat taaccaagtt
     1021 atagtttccg gaaacggtta acaaccaata atataaatat aacacgatat tacttgacat
     1081 cgtcttgaaa atcactatat ttgttcaact aataactcat tatgtcaaac aactaactga
     1141 cctgtcatga agaagcatta catttttacc tgtttcattg ccatcttcct tacgctgccc
     1201 ttgctgaacc tttatgccca gaagcccggg atctactttg agccttcatc cctcgccgaa
     1261 acgctcgccc ccgacgattc cgtggtggtc cattccgttc tgcacaacgg ttcggcccag
     1321 acagttgagt tctcttttcc gggatacgtg acccgcgacc tgggggggcc ggatgatttc
     1381 ggctattcct ggatcgatag cgaagagccg ggtggtcccg actggtcatg gactgatatt
     1441 tcagaagtgg gaattttagt ggaaggattg ggagacgatg aaatggcggg accgtttgag
     1501 atgggtttca acttcagctt ctacggccag accaagaatc atttctggat cagtccgaat
     1561 ggcgtgatca gcttcaacga acagtttatt ccatatgcaa acgagcctat tcccaccaat
     1621 gataattata acgattttat cgcctggttc tgggacgacc tgaagatcga ttcggcgatt
     1681 acaagagttt attataaaaa ctatgaagag aaaaccgttg tgcaattcac taaaatggtc
     1741 cattatcccg gtaccgaatc atttattacc ggccaggtca tcatgatggt caacggttct
     1801 atcctattga aatacaggct ggtcagcgag gattttgaga caaccggagc gacggcagga
     1861 atccagtcat ggaactccca gcagggattg caggttgtgt acaatgctga atatgtccac
     1921 tccgaactgg ctgtccgatt tgacctgaac cggaatttca tcacctctgt taatccttcc
     1981 accctgacac ttccgccagg aacacaggaa acgatctgga tcacttactc ttcgactggc
     2041 tttgaagtcg gatcctatga gcaagacctg aaatgcatta ccagccatcc ggaattgcct
     2101 cacctgatcc ttcacaatgt catgcatgtc aatgttcctg tcaattcagg atttaaagga
     2161 tatgtgaccg atgcggcaac aggtttcgcc atccatgaag tgatggtgaa agcgggtgaa
     2221 caggttactt acacaaacga taatggttat tatgaattac ccctggagga aggcgtttat
     2281 aatgtcaaat tccagcgaaa c
//
LOCUS       DAOQXS010000460         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53007_length_2294_cov_2.936579, whole genome shotgun sequence.
ACCESSION   DAOQXS010000460 DAOQXS010000000
VERSION     DAOQXS010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53007_length_2294_cov_2.936579"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKM34_11105"
     CDS             complement(<1..191)
                     /locus_tag="PKM34_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNQ84184.1"
                     /translation="MTKKITIEPVTRVEGHGKVTIHLDEQNNVAQTRLHIVEFRGFER
                     FVQGRPYWEMPVLVQRLCG"
     gene            complement(208..756)
                     /locus_tag="PKM34_11110"
     CDS             complement(208..756)
                     /locus_tag="PKM34_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84185.1"
                     /translation="MSKAIVATTSMAGCFGCHMSILDIDERILDLIELVEFNKSPIDD
                     IKKFTKQCDVGILEGGCCNSENVEVLKEFRKNCKILISVGECAIMGGLPALRNGIPIQ
                     ECIEEAYLNGPTVGLNKDRILPNDDELPMLLDKVYPCHEIVKVDYFLPGCPPRADLIW
                     NALVALLTGDEMKLPYEVVKFD"
     gene            complement(781..1482)
                     /locus_tag="PKM34_11115"
     CDS             complement(781..1482)
                     /locus_tag="PKM34_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNQ84186.1"
                     /translation="MSEKIRFIIDDKVCYAKPGQTIMDAARDNGIFIPSLCYLEGIKP
                     AGSCRICNVKVNGRNMTACTTPVAEGMVVENNLPDLEDMRKAIVEVLFVAGNHFCPSC
                     EKSGNCELQALGYRYKMMVPRFPYEFPVKGVNAKAKYIYHDRNRCILCKRCIRTVMKD
                     DKPVFAFNQRGGHHLHIEMDIELANSLTEAEALHAMDICPVGAIIKKERGYYSPIGSR
                     KYDFDEIGADLVKKV"
     gene            complement(1510..>2294)
                     /locus_tag="PKM34_11120"
     CDS             complement(1510..>2294)
                     /locus_tag="PKM34_11120"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNQ84187.1"
                     /translation="ALIESMEGKRGEPRNRPPFPVQKGYLDMPTVINNVETLSAVVKI
                     MVKGADWFKAMGTKESAGTKLLSVAGDCKHPGVYEIEWGTTIRELLEMVGATSVQAVQ
                     VGGPSGVCINPTQFHREIAFEDLATGGSMIIIGKQRNLLKEVVVNFMDFFIEESCSSC
                     VPCRSLTVILKNKLQKILDGKGSRLDVNELYEWTKYMKAANRCGLGQTAANPVLSTIE
                     NFRQLYDDLVIPEEEFVSTFNLEAAVADSCAYVGRIPKFEHH"
BASE COUNT          529 a          607 c          537 g          621 t
ORIGIN      
        1 atgccgcaga gccgctggac cagcacgggc atttcccagt agggcctgcc ctgcacgaaa
       61 cgttcaaatc cccgaaattc gacaatatgt aaccgggttt gggcgacatt gttctgctca
      121 tccaggtgga tggtgacttt tccgtgccct tccaccctgg tgacgggttc tatcgtgatt
      181 ttctttgtca tgtcctaatc ctccaaatta atcaaattta acaacttcat aaggcagctt
      241 catttcgtcg ccggtcagca aggcaaccag ggcattccag atcaggtcgg ccctcggtgg
      301 gcatcccggg agaaaataat ctaccttcac aatttcatgg cagggataga ccttgtcgag
      361 aagcatcggc agttcatcgt catttggaag gatcctgtcc ttgttcagcc ccacagtagg
      421 cccattcaaa taagcctctt cgatgcattc ctggatcggt atgccgttgc gcagggccgg
      481 caatccgccc ataatggcgc actcaccgac ggagatcagg attttgcagt ttttgcggaa
      541 ctctttcaga acctcgacat tctcactgtt gcagcatccc ccctccagga ttcccacatc
      601 acactgcttg gtgaactttt taatatcatc gatcggcgat ttattgaatt cgaccagttc
      661 gatcagatcg aggatacgtt cgtctatatc aaggatcgac atatgacagc caaaacagcc
      721 ggccatggat gtcgttgcta caattgcttt gctcatattc agacgtatta aatgttttac
      781 ttatactttt ttaaccaggt cagctccgat ttcatcgaaa tcatactttc tcgatccgat
      841 cggggaataa taccctctct cttttttgat gatggctcca accgggcaaa tatccattgc
      901 atgcagggct tcggcttctg tcaggctatt ggccagttca atatccatct cgatatgcag
      961 gtgatggccg cctctctgat tgaaagcaaa tacgggctta tcatctttca taacggttcg
     1021 gatacagcgc ttacacagaa tacaccggtt cctgtcgtga tagatatact ttgctttggc
     1081 attgacaccc ttcaccggga actcgtaagg gaatcgcggc accatcattt tgtagcggta
     1141 tccgagagcc tgcaattcgc agttcccgct tttttcgcaa gaagggcaga aatggttgcc
     1201 ggctacgaac aaaacttcca cgatggcttt ccgcatatct tccaggtctg ggagattgtt
     1261 ttccacgacc attccttcgg cgaccggggt tgtgcaggcc gtcatattcc ggccattgac
     1321 cttcacattg caaatccggc atgaacctgc cggtttgatc ccttccaggt agcaaaggga
     1381 aggaataaaa atgccattat cgcgggcggc atccattata gtctggcccg gtttggcgta
     1441 acagacttta tcgtctatga tgaatctgat tttctcgctc atattccaat ttctttaatg
     1501 attgctgact cagtgatgtt cgaatttcgg gattctgcct acataagcgc aggaatcggc
     1561 cacagcggct tccaggttga aagtggaaac gaattcctct tccgggatga ccaggtcatc
     1621 atacaactgg cggaaattct cgatggtgga aagtaccggg ttggcagcgg tctgtccgag
     1681 accacagcgg ttcgccgctt tcatgtattt agtccattcg taaagttcat tgacatccag
     1741 ccgggaacct ttgccgtcga ggatcttctg aagtttattc ttcaggatga ccgtcagtga
     1801 gcggcaagga acacaagagc tgcatgattc ctcaatgaaa aagtccataa aattcacgac
     1861 aacttctttc agcaggttac gctgtttgcc gatgatgatc atcgagccgc cggtagccag
     1921 gtcttcaaaa gcaatttcac gatggaactg ggtcggatta atgcacacac ccgatggtcc
     1981 gcccacctga acggcctgta ccgatgttgc tccgaccatt tccagaagtt cacggatggt
     2041 ggttccccac tcgatctcat agaccccggg atgtttgcag tcgccggcga cactgagcag
     2101 tttggtgccg gccgattctt tcgtgcccat ggctttgaac cagtcggcgc ctttcaccat
     2161 gatcttcacg acagccgata atgtctctac attattaatg acagtaggca tgtccaggta
     2221 tcctttctgc accgggaatg gcggacggtt gcggggctct ccccttttcc cctccatcga
     2281 ctcgatcaga gcgg
//
LOCUS       DAOQXS010000461         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53108_length_2291_cov_2.942755, whole genome shotgun sequence.
ACCESSION   DAOQXS010000461 DAOQXS010000000
VERSION     DAOQXS010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53108_length_2291_cov_2.942755"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PKM34_11125"
     CDS             complement(<1..1648)
                     /locus_tag="PKM34_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ84188.1"
                     /translation="MAVNLFKKRFLRLFFLTIPFILLTCFTFSVSGQETETSEIIMPD
                     SLTTADSISPVGLQLINDATQAEKIVNESVTIGMLDSLASIPYFKEYYFNTDTAILNV
                     YDFPNGHVPVYPDSVYKARIEKLDQETPLDLTYNESVKSFIELYAVRKRMLTSKVLGL
                     KEIYFPLFEEYLDKYDIPLEMKYLAIVESALNPTAGSRAGAKGLWQFMYHTGKLYGLK
                     VTSMTDDRFDPYLATDAACRHMRDLYAMFGDWNLVMAAYNSGAGNVTKAIRRAGGLKD
                     YWAIWPFLPKETRGYVPAFIAVNYVMSYAPEHNLYPLDPGILYNGIDTVVVTQPLAFD
                     QLSEFLNISKEELAFLNPIFKSDLIPAYNGKTFSLRLPRELAVKFAENEQAIYNYKSR
                     SGLEKEKLISEIKKATDRQYHTVRTGESLGLIARKYGVSVKQIQQWNNMKGTMIHPGQ
                     QLIIMPSSGYASTTPSSPSTSKAAKPTGDPPAAEQKNQEATPEPVTQPEPEFHTVRSG
                     ESLAIIAQKHHCSVDDLKKWNNLSSNMIHPNQKLKISGSKP"
     gene            complement(1820..>2291)
                     /locus_tag="PKM34_11130"
     CDS             complement(1820..>2291)
                     /locus_tag="PKM34_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNQ84189.1"
                     /translation="LIKNVYDRQDKIIENRDKLEEMLVRAHVEAKKAWDLGATEDQMK
                     PILMDIRHAQWRWDYAAASHGASFHAPVEIGRVVSTGITIAQDGRIKLARLLASLGHN
                     EEIPYPDIATKEKAQAFIGLDMAKLEAEKQVFLNEVVPEWDRKAAEREATYPTQ"
BASE COUNT          530 a          529 c          600 g          632 t
ORIGIN      
        1 atggtttact gccggaaatc ttcaacttct ggttcgggtg gatcatgttc gacgagaggt
       61 tattccattt cttcagatcg tcgaccgagc aatgatgttt ctgagcgata atggccaggc
      121 tctccccact tctgaccgta tggaattccg gttccggctg tgtgaccggt tccggggtgg
      181 cttcctggtt tttttgttcc gctgcgggag gatcaccggt cggttttgct gctttggagg
      241 tggaaggaga cgaaggcgtg gtggaggcat acccgcttga aggcataatg ataagttgct
      301 gtcccggatg gatcatcgtg cctttcatgt tattccactg ctgtatctgt ttgacgctta
      361 cgccgtattt ccgggcaata aggcccaggc tttccccggt tctgaccgtg tggtactgcc
      421 tgtcggtggc tttcttgatt tcggagatca gtttttcttt ttccaggccg ctccggcttt
      481 tataattata aatagcctgt tcattttcag cgaatttcac cgctaattcg cgcggcaacc
      541 tcaaactgaa tgttttccca ttatacgccg gaataagatc cgatttaaag atgggattca
      601 ggaaagccag ctcctccttt gagatgttca gaaactcgga taactggtcg aaagccagcg
      661 gttgggttac gaccacggta tctattccat tgtaaagtat tcccggatca agcggataaa
      721 gattatgttc gggggcgtag ctcatgacat aattcactgc gataaaagcg gggacatatc
      781 ctcttgtttc tttgggcagg aagggccaga tggcccagta atccttaagt ccgcccgccc
      841 ggcggatggc tttggtgaca tttccggcac ccgagttata ggcagccatc acgaggttcc
      901 aatcgccgaa cattgcatag agatcgcgca tatggcggca tgcggcgtca gtggcaaggt
      961 aggggtcgaa ccggtcgtcg gtcatcgagg tgaccttcag cccgtatagt tttcccgtat
     1021 ggtacataaa ctgccacaaa cctttggcgc cggcgcggga acctgcggta gggttgaggg
     1081 ccgattcgac tatggccagg tacttcattt cgagcgggat atcatattta tccaggtatt
     1141 cttcaaaaag tgggaaatag atctctttca atcccaacac cttagatgtc agcatccgct
     1201 ttctgacagc atatagttct ataaaagatt ttacagattc attataagta aggtcaagag
     1261 gcgtttcctg gtccagcttt tcaattctcg ctttatacac cgaatcggga taaaccggca
     1321 catgaccgtt gggaaaatca tagacgttca ggattgcggt atccgtatta aaataatact
     1381 ccttaaaata agggattgaa gccaggctgt cgagcatccc gatagtcacc gattcattca
     1441 cgatcttctc tgcctgggtg gcgtcattga tcaattgtaa tcccaccggg ctgatcgagt
     1501 cggcagtggt aaggctgtcc ggcataatta tctcgctggt ttccgtttcc tggccggata
     1561 cggaaaaagt aaagcaggtc aataaaatga acgggattgt aaggaaaaat agtcgtaaga
     1621 atctcttctt aaataaatta acagccatca atgtcgtttt taatgctttt ggctataaaa
     1681 ataaccaacg aatacccggt agcagggtat tgaagtgcag aaatattaac acggcaaagt
     1741 taagaaaatt gcaccgcgga ttacaaaata tctggtattt ggttcgtgct ccgacgacta
     1801 aagccgtcgg ttcggtggat tactgcgtcg ggtaagtagc ttcccgttcg gcggctttgc
     1861 ggtcccattc ggggacaact tcattcagga agacctgttt ttcggcttca agttttgcca
     1921 tgtcaaggcc gatgaaggcc tgggcttttt ccttggtggc aatatcgggg taagggatct
     1981 cctcattatg gccaagggaa gcgaggaggc gggccagctt gatccggccg tcctgggcga
     2041 tagtaatgcc ggttgaaacg acccggccga tttcaacggg agcatggaag gatgcgccat
     2101 ggctggcggc agcataatcc cagcgccact gggcatgacg gatgtccatc aaaatgggtt
     2161 tcatctggtc ttctgtcgct ccaaggtccc aggctttttt agcttccaca tgggccctga
     2221 cgagcatctc ctccagtttg tcgcggtttt cgatgatctt atcctggcgg tcatagacat
     2281 ttttgatcag g
//
LOCUS       DAOQXS010000462         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53374_length_2284_cov_3.668910, whole genome shotgun sequence.
ACCESSION   DAOQXS010000462 DAOQXS010000000
VERSION     DAOQXS010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53374_length_2284_cov_3.668910"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKM34_11135"
     CDS             complement(<1..457)
                     /locus_tag="PKM34_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ84190.1"
                     /translation="MKTIYIVRHAKSSWNQADLPDERRPLMEKGKKRTKKIIDKLHEM
                     NIHVDYIISSHAVRALETAKLLAHALNYPNENIKIDPHIYFTDGDGIINQFFDIPERF
                     RSVMIVGHNPALTDFINQYLKEPIENLPTSGIVGISFETDKWEEIPLAKH"
     gene            695..1681
                     /locus_tag="PKM34_11140"
     CDS             695..1681
                     /locus_tag="PKM34_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNQ84191.1"
                     /translation="MKKLLLSALFVLPFLSLFSQDCSDLFISEYVEGSGNNKAIELYN
                     PTNNPINLSDYYLVRYRNGLFTPDKIQLGGTIAPKSTFVAVLDKRDPGGTGQEIMVDL
                     ALQAKADTFLCPVYDVNKMMYFNGNDAVTLEKITGDIVDIFAHIGPPMLEDDEGWGSI
                     TDTTITYNSGGVPTQYTISDYIVGPLFWLCWTSDNTLIRKSSVKNGVKTNPDVFVVTT
                     EWDSIPENTFDSLGFHRCDCNTSGISDPALDKKVRIYPNPSLNRTITITGEDFITSIE
                     VIDITGRLTDVIELTGRETGYTYEFGERMHGICFIRVRMEDNSVYTERIFIR"
     gene            1690..>2284
                     /locus_tag="PKM34_11145"
     CDS             1690..>2284
                     /locus_tag="PKM34_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ84192.1"
                     /translation="MTGRTFILLCLLQFPLIHIFAQQQTVTGIITDNATKELLIGVNI
                     TYAPGKGTVTDVNGKYFLRLDPGDYELEFSYVGYEKQTMRVKVADKSVYLDVSLKNVT
                     LTEIEVVGDVARTRETPIAFSNIPPMKIEEELASRDLPMILNATPGVYATQKGGGDGD
                     SRINIRGFSQRNVAVLLDGIPVNDMENGWVYWSNWFGL"
BASE COUNT          609 a          515 c          534 g          626 t
ORIGIN      
        1 gatgttttgc cagcggaatc tcttcccatt tatccgtttc gaatgaaatg cctacaatcc
       61 ctgatgtggg cagattttca atgggttcct taaggtattg attgataaaa tcggtcaggg
      121 ccgggttatg gcccacgatc atcactgagc ggaaccgttc cggaatatca aagaactggt
      181 ttatgatccc atcgccatcc gtgaaataaa tgtgcgggtc gatcttaatg ttctcgttgg
      241 gataattcag ggcatgagcc agtaacttcg ccgtttcaag agcccttacc gcatggctcg
      301 aaatgatata atccacatgg atattcattt cgtgtagctt gtcaatgatt ttttttgtcc
      361 ttttttttcc tttctccatc agcggacgcc gttcatccgg caaatcggcc tgattccacg
      421 aagatttcgc atgccttaca atgtagatgg ttttcatctt aaaaggttga tttggcgacg
      481 aaaatacatt tacgtcatac aggtataacg atgatgaagt taataaatta ttaaaattcc
      541 catttctttg ccggggtatt tacatcaatt atggaattgc gacttaccaa tttgcttcat
      601 aagcaaatat tatccaagat ttatcaactg cctgaatgga tttaggaaag gaatattatt
      661 tttgtaaatt cagaaatttc aaatcaatcc tcatatgaag aaacttttac tttcagcact
      721 ttttgtgttg ccttttttgt cgctgttttc gcaggactgt tccgacctgt tcatctcgga
      781 atatgtcgag ggatcaggca ataataaagc catcgagtta tataacccga ccaataatcc
      841 gatcaattta tcggactact acctggtgcg ttaccgtaat ggccttttca ccccggataa
      901 aatccagctt ggcggcacta ttgcgcccaa gagtaccttt gtcgccgtcc ttgataaacg
      961 tgatccgggt ggcaccgggc aggagatcat ggtcgacctg gcactccagg ccaaggccga
     1021 tacattctta tgccctgtat atgatgttaa caaaatgatg tattttaacg gcaacgacgc
     1081 ggtgaccctg gaaaagatca ccggcgatat cgtggatatt tttgcccata tcggcccacc
     1141 catgctggaa gatgacgagg gctggggaag catcaccgat acgacgatca cttacaacag
     1201 cggtggagta ccgacgcagt acacgatcag tgattatatt gtcggaccgc tcttctggct
     1261 gtgctggact tccgataata cacttatccg gaaatcatcg gtgaaaaatg gagtgaaaac
     1321 caatcccgat gttttcgttg tcacgacgga atgggactcc atccctgaaa atacgttcga
     1381 ctcactggga ttccaccggt gcgactgcaa tacatccggc atcagcgatc ctgctttgga
     1441 taaaaaggtc cggatctacc caaacccttc cctgaacaga accataacca ttacaggtga
     1501 agatttcatc acatccatag aagtaattga tattaccgga aggctgaccg atgtcattga
     1561 actcacgggc agggaaaccg gttatacata cgaatttggc gaaaggatgc atgggatctg
     1621 ctttatcagg gtccggatgg aagataattc agtttatacc gaacggatct ttatcagata
     1681 acttgcagga tgaccggaag aacctttatt ttactctgtt tactacaatt ccccctcatt
     1741 catatttttg cccagcaaca gacagtgaca gggatcatta ccgataatgc aaccaaggag
     1801 ttgctgatcg gggtcaatat tacctatgca ccggggaaag ggactgttac cgatgtgaac
     1861 gggaaatatt ttctcaggct tgatcccggg gactacgagc tggaattttc atacgtgggt
     1921 tatgaaaagc agactatgcg ggtgaaagtt gccgataaaa gtgtttatct cgatgtttcc
     1981 ctgaagaatg tcaccctgac ggagatagaa gttgtgggcg atgtagcccg gacgcgtgaa
     2041 acgccgatcg cattttcaaa catcccgccg atgaagatcg aagaggagct ggcttcacgc
     2101 gacctgccga tgatcctgaa tgccaccccc ggtgtctatg cgacccagaa agggggaggg
     2161 gatggcgatt cccggatcaa tatccgcggg ttcagccagc gtaatgtggc agtgctcctc
     2221 gacggtatcc cggtgaacga tatggaaaac ggctgggtat attggtctaa ctggttcggc
     2281 cttg
//
LOCUS       DAOQXS010000463         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53423_length_2283_cov_3.070916, whole genome shotgun sequence.
ACCESSION   DAOQXS010000463 DAOQXS010000000
VERSION     DAOQXS010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53423_length_2283_cov_3.070916"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="PKM34_11150"
     CDS             complement(<1..1354)
                     /locus_tag="PKM34_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNQ84193.1"
                     /translation="MKKIIVLFVLLFAVFQSFAAEWTAIRSNNPAPGQKILISSGIDQ
                     SVITFKTDGFYSTTVNTPQGPAVVISLDEATPLLLQGAPDLPKLTASVIIPDLAEMQI
                     EVISAQYQDFENIFVAPGKGNLTRDIDPSSVPYTFGPEYSTNAFFPGNLAGTREPYII
                     RDYRGQTVIAYPFQYNPVTRTLRVYTDITVKISSTGHLGENPLYRNENDESIDAGFNA
                     VYQRHFLNAMENGSRYNPVPEYGNMLVICYSAFLDAIQPYVEWKKQIGYPVEVVDVAT
                     IGNSNAIKSFIANYYNTKGLTFVLLVGDAAQVPASSTSAGDSDNNYAYVVGSDHYPDL
                     FIGRFSAENVNQVQTQVQRTIMYEQNPPVSADWFSVCTGIASNQGPGDDNEMDYEHIR
                     NISNNKLIPFTYTYANELFDGSQGGNDEGGNPTPTQVAADVNAGTSIINYTGHGSDGS
                     W"
     gene            complement(1417..1971)
                     /locus_tag="PKM34_11155"
     CDS             complement(1417..1971)
                     /locus_tag="PKM34_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84194.1"
                     /translation="MKIFQTLINLINATAIVAFLFPLNSFSQEWERYEIDISECSVVL
                     PSEPEWYYEYSQDSSYVWSGEVQQGNMFFGVICIEFAVPFDTEEFDEYDLILIAQDYL
                     DYLKDQFSITSSTGYEPVSWIDSETGGTGISDYWTDAEGDPWAVQCWIDPYNLAVLYI
                     YSDPETDFSDYEEHFFNSFWFPEE"
     gene            complement(2003..>2283)
                     /locus_tag="PKM34_11160"
     CDS             complement(2003..>2283)
                     /locus_tag="PKM34_11160"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNQ84195.1"
                     /translation="LVQQGNISLIEALKAGALIEDKDIFDLESDMNQTENPAILNTFS
                     RLSCASGNHLHSFTKWLTSKGITYVPQFLSQQEYDEIITLPHQFCGNR"
BASE COUNT          582 a          516 c          605 g          580 t
ORIGIN      
        1 cccatgaacc gtcgctgccg tgaccggtgt agttgatgat gctggtaccg gcgttcacgt
       61 cggctgccac ctgcgtcggt gtcgggttac cgccttcgtc attgccgccc tggctgccgt
      121 cgaacagttc gttggcataa gtgtatgtga aggggatcag cttgttgttg gagatattgc
      181 ggatatgttc gtagtccatc tcgttgtcgt cgccgggacc ttggttggag gcgatgcctg
      241 tgcagacgga gaaccagtcg gcggagaccg gcgggttctg ttcgtacatg atggttcgtt
      301 gcacctgtgt ctgtacctgg ttcacgttct cagcggagaa ccggccgatg aaaaggtcgg
      361 ggtaatgatc gctaccgacg acgtaagcgt agttgttatc ggaatcgccg gcggaggtgg
      421 atgatgccgg cacctgtgcg gcgtcgccca ccagcagcac gaaagtcagg cctttggtgt
      481 tgtaatagtt ggcgataaaa ctcttgatgg cattggagtt gccgatggtt gccacatcca
      541 cgacctccac cgggtagccg atctgctttt tccattcgac ataaggctgg atggcatcca
      601 ggaatgcgct gtaacaaatg acgagcatgt tgccgtattc cggaaccggg ttgtaacggg
      661 agccgttttc catggcattt aaaaagtgcc gctggtaaac ggcgttgaaa ccggcatcga
      721 tagattcgtc attttcgttt ctatacaaag gattttcacc caaatggccg gtggagctga
      781 ttttaaccgt aatatcggta taaacgcgga gggtacgggt caccgggttg tactggaagg
      841 ggtaggcaat cactgtttga ccgcggtagt cacggatgat atagggttct cttgttccgg
      901 ccaggttacc cgggaagaag gcattggtgg agtactcggg tccgaaggta taaggtaccg
      961 acgacggatc gatgtcgcgc gtcaggtttc ctttgccggg agcgacaaag atattttcaa
     1021 agtcctggta ttgagcggaa atcacctcaa tctgcatctc cgccaggtca ggaatgatga
     1081 cggaagcggt aagtttcggc aggtcgggag caccctgcag caacagggga gtcgcttcat
     1141 caagtgagat aacgacagcc gggccttgcg gggtattgac cgtggtggaa taaaatccgt
     1201 cggttttaaa agtgataaca ctttgatcga taccggaaga gatcaggatt ttttgtcccg
     1261 gggcagggtt gttggagcgg atggcagtcc attcagcagc aaacgactga aatacagcaa
     1321 acagcaatac aaagagcaca ataatttttt tcatcgggtt aatgaattta aaacgagttc
     1381 aaaggtacta aaaaaagggt tggaaaccct ttctttttac tcctccggaa accagaagct
     1441 gttgaagaag tgttcctcat aatccgaaaa atcggtttcg ggatcgctgt aaatatagag
     1501 caccgcaagg ttgtatggat cgatccagca ttgaacggcc caggggtcgc cctctgcgtc
     1561 ggtccagtag tcggatattc cggttccccc ggtttctgaa tctatccatg agacaggttc
     1621 ataaccggta gaagaagtta tactgaattg atctttaagg taatccaggt aatcctgagc
     1681 aatcaggatg agatcatatt catcaaactc ctccgtatca aaaggcacgg caaactcgat
     1741 acagatcaca ccgaagaaca tattcccctg ctgcacctca ccgctccaga catagctgct
     1801 gtcctgtgaa tattcgtaat accactccgg ctctgaaggc aatacaaccg agcattcgct
     1861 gatatcgatc tcataccgtt cccattcctg ggagaacgaa ttcagcggaa ataaaaaagc
     1921 cactatagca gtggcattta taagattaat aagggtttga aaaatcttca tggtgcgatt
     1981 tttcagtaaa ggtataaaaa ctttaccggt tgccacagaa ctggtgaggc aaagtaatta
     2041 tttcatcgta ctcctgctgg cttaagaatt gcggcacata cgtaatccct ttgctggtaa
     2101 gccattttgt aaatgaatgc agatgattcc ctgaagcaca cgacaacctg gaaaatgtat
     2161 ttagaatcgc cggattttca gtttggttca tgtctgattc caaatcaaag atatctttat
     2221 cctcaatcaa cgcaccggct ttaagtgcct caatgagaga aatattaccc tgttgcacaa
     2281 ggc
//
LOCUS       DAOQXS010000464         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53541_length_2279_cov_4.467626, whole genome shotgun sequence.
ACCESSION   DAOQXS010000464 DAOQXS010000000
VERSION     DAOQXS010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53541_length_2279_cov_4.467626"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="PKM34_11165"
     CDS             <1..1920
                     /locus_tag="PKM34_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNQ84196.1"
                     /translation="YGKAIFIEDNLTITPTACAGSSGSIEGLVVEGTEPLAFEWYDGD
                     GNLVADALDIFNLSVNNYYLHVLDGNGCETVSEAYTIADAGDIEISEVDHIPGHCGQQ
                     NGFIEITATTGSGTQLQYSIDNGNNWQTSNQFADLPAGTYFIKVGDQVGCETVYAGNP
                     VVIENIPGPEITDVTTTPEYDYQSDGSIDIEALIGSGNLYYSVDNGSSFQTNNGLFEN
                     LSAGTYTCVVKDDFGCDTSFIVIIDRILSQVIDAIAGDGYTCIGDATASQLLLNNFTE
                     VDSFHVQLTYDPAVIQCDGYIQVHPDLESGLQASIIPVLGEVHITWKGETPATLPENA
                     NMLELVFSGLEEGVSQVDWIAEPGEGQFFNVYGEENSVNYELGTIRIFTRPEIILGAE
                     LHVCEGDNIYLPAFVMGGTGEYDYYWTGPSNFSSTDKELKITGIQAYQAGDYQLTVTD
                     TIDCTSQKSMELIVSENPIAAFHGTDTLVVDSGYILEAGTGQAHYLWNTGDSTESIAI
                     YSEGMYTVGMESQAGCIGSDSVYVVLREEPVVEPSQYLYIPNAFTPDGDGRNDVFRAI
                     PSVNTISHFSMRVFDRWGGQVFESEDINQGWDGTKNGKPCPEGAYVFRITFNVEGIPG
                     LEGEQTIVGTVVIVR"
BASE COUNT          618 a          497 c          596 g          568 t
ORIGIN      
        1 tacggcaagg caatttttat tgaagacaac ctgaccatca cacccacggc ctgcgcggga
       61 agcagtggca gtatagaagg gctggtagtt gaaggaaccg aaccgcttgc ctttgaatgg
      121 tatgatggcg acgggaacct ggtggccgac gcactggata tatttaacct gtcggttaac
      181 aattactatt tgcacgtact tgatggcaat ggttgcgaga ccgtgtccga agcctatacc
      241 attgccgatg ccggggatat tgagatatcg gaggttgacc acatccccgg acattgcggc
      301 cagcaaaacg gcttcatcga gatcacggcc actaccggct ccggtacaca gttgcagtat
      361 tctatcgaca atggcaacaa ctggcagaca agcaaccagt ttgctgacct gcctgcgggc
      421 acttacttca ttaaagtcgg cgaccaggtg ggctgtgaaa ccgtttatgc cggcaacccg
      481 gtggtgatcg aaaacattcc cggcccggag atcacggatg ttactactac accggaatac
      541 gactaccagt ctgatggaag cattgacatt gaagcgctta ttggaagtgg taacctttac
      601 tattctgtag ataacggaag cagcttccaa acaaacaatg gtctttttga gaatctcagt
      661 gcaggaacat acacttgtgt ggtaaaagat gactttggtt gcgatacttc ttttattgtg
      721 atcattgaca ggatactctc tcaggttatt gatgccatag caggagatgg ttacacctgt
      781 atcggtgatg ctacagccag tcaattgctg ttgaataatt ttaccgaagt tgacagtttc
      841 catgttcaac tgacctacga tccggccgtc attcaatgtg atggatatat acaggtccat
      901 cctgatctgg aaagtggatt gcaggcaagc attatccctg tgttaggtga agtgcatatc
      961 acatggaaag gagaaacgcc cgccaccttg cctgaaaatg ccaacatgct ggaactggta
     1021 ttcagcggcc tagaggaagg ggtatcacag gtggactgga tagctgagcc gggcgaagga
     1081 cagttcttca atgtttacgg agaagagaat tctgtgaatt acgaactggg aacgatccgg
     1141 atcttcacac ggccggagat catcctggga gcggaattac atgtatgtga aggggataac
     1201 atctacttgc cggcatttgt catgggtggt acaggagagt atgattacta ttggacaggc
     1261 ccttcaaact tttcaagcac cgacaaagaa ctgaaaataa cgggaatcca ggcataccag
     1321 gctggagact accagctaac ggttacagac accattgatt gcacaagcca gaagagcatg
     1381 gagctgatcg tcagcgaaaa tcccattgca gcattccatg gcacagacac cctggtggtg
     1441 gattcgggct acatcctgga ggccggcacc gggcaggcgc attacctctg gaacactggc
     1501 gatagcaccg aaagcatcgc gatctattcg gaaggcatgt acactgtcgg gatggagtcc
     1561 caggccggct gcatcgggag cgattcggtc tatgttgtac ttcgcgaaga gcctgtggtc
     1621 gagccttcgc agtatttata catccccaat gcttttacac cggatgggga cgggaggaac
     1681 gatgtgttca gggcaattcc cagtgtcaat accatttcgc atttctcaat gagggtgttc
     1741 gacaggtggg gtgggcaggt gtttgaatca gaggatatca accagggatg ggatggcact
     1801 aagaacggaa aaccctgccc cgaaggggca tatgtcttcc ggatcacatt taatgttgaa
     1861 gggattcccg ggcttgaagg agaacaaacc atagtcggaa ccgtggtgat cgtgagatga
     1921 tcttcctgat ggctatctgc actttccaaa aatcatcata aaaattattg gggctaacgg
     1981 ttttgtgtat ggctcgttgc ggattagaca gcgagttttt tcggttaagc agtaaggtta
     2041 gtaaaaaagc aataactttg agttatacac ttcagccgca atgagctata cacgttgtta
     2101 cctgctggtt ttcaatcatc aatccttaaa aattctcctg ccatatcaaa gacaagcaca
     2161 agattgttgt caagttgaac ttcttgattt cctgattgaa gttcccatcc tatgataaag
     2221 tttgttgagt agtttgagat tacataggaa agaatattag ctggaataac gctgtcagg
//
LOCUS       DAOQXS010000465         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_53839_length_2271_cov_3.619585, whole genome shotgun sequence.
ACCESSION   DAOQXS010000465 DAOQXS010000000
VERSION     DAOQXS010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53839_length_2271_cov_3.619585"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKM34_11170"
     CDS             <1..337
                     /locus_tag="PKM34_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ84197.1"
                     /translation="TEQLHNFARFLNMLMKQKLSEYHTGYRAFSKEVLQNIKYQHNSD
                     DFIFDNQMLAQIFFAGYEIAEVTCPTKYFDDASSINLKRSMKYGLGVLGVSFRYFLHK
                     HRIARYRIF"
     gene            complement(419..>2271)
                     /locus_tag="PKM34_11175"
     CDS             complement(419..>2271)
                     /locus_tag="PKM34_11175"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84198.1"
                     /translation="WGNLTVHLVLTESEIVYSWQGQDHLNFVERLMAPTHMGTVVDFT
                     SQDEVTLDLSFTMDASWVASNCELVAFVQNEATKEVLQGNMVAIPDLEPMQATAGFTC
                     EDVTPCITTAVEFEDQSMGEIISWNWTFEAGNPATSAAQNPVVTYNTQGQYDVQLIVY
                     DGTVYDTLLNPNYILVITPPVQPQTPNGPTTVCQENDGIQYSTPLVQWATTYVWDVEP
                     DAAGTISGPDPVATFTLAPGYLGTYTIKVRADNDCGNGTWSQGLNCAAYMTPSEYTLS
                     DGSGYCEGSDGIELTLDGSQNGVDYELYLDGDPTGQIMAGTGSAITFGNQTEQGIYTC
                     VGYTDYCDKNMIGNAYIFIITPPDEGETPSGETQVCSSDSGTEYISDDIDGATSYFWN
                     LSPTEAGSISGNDETATVDWNAEFSGDAYISVAGVNSCFTGDFSDALTVVVNAAPQPV
                     ISGPQLVCDYEVGDIYSTELADGNDYTWEINGGTITEGNGTNEITVTWGAAGDGWVKV
                     TETNGVCSFTTLEYEVVIDECIGVGENGMASFMIYPNPVKEEMMISFTGKTTNCRMVI
                     LNQLGQVMIDQMTGNNKQISVNTSMLSKGLYSIVVYTDNGLIEKKFVKTE"
BASE COUNT          554 a          589 c          533 g          595 t
ORIGIN      
        1 cacggaacaa ctgcataatt ttgcccggtt cctgaacatg cttatgaagc aaaagctatc
       61 ggaataccat accggctaca gggctttttc gaaggaggtt cttcagaata tcaaatatca
      121 gcataactcc gacgatttta tcttcgataa ccagatgctg gcccaaatct tctttgccgg
      181 gtatgagatt gccgaagtca cctgcccgac caaatatttc gacgacgcct cttccatcaa
      241 cctgaaaaga agcatgaaat acgggctggg cgtacttggg gtttcattcc ggtacttcct
      301 gcataagcac aggattgccc ggtacaggat cttctaaaac agagatccct cactgcgttc
      361 gggatgacga tgcgtcacga acacagtgag ggatcttact caaaaatgtt ttacaggtct
      421 attccgtctt aacgaatttc ttctctatca atccgttatc tgtatagacc acaatggaat
      481 acaggccttt ggacagcatg gaggtgttga ctgaaatctg tttattgtta cccgtcatct
      541 gatcaatcat cacctgtccg agctggttca ggataaccat gcggcagttg gttgttttac
      601 ccgtaaagct gatcatcatt tcctccttaa cgggattagg atagatcatg aaagaagcca
      661 ttccgttttc tccaacgccg atacattcat cgatgaccac ttcatattcg agggtggtaa
      721 aagaacaaac gccattggtt tctgtgactt tcacccagcc atcgccggca gcgccccagg
      781 tgacggttat ctcatttgtc ccgttgcctt cagtaatggt cccgccgttg atctcccatg
      841 tgtaatcatt accatcggca agttctgtcg agtaaatgtc gcccacctca taatcacaaa
      901 ccagttgcgg cccactgata acaggctgcg gagcggcatt gaccaccacg gtgagggcat
      961 cggagaaatc gccggtaaag cagctattca caccggcaac gctgatataa gcatctcctg
     1021 aaaattccgc gttccagtcg accgtggctg tttcatcatt tccactgatg gagcccgcct
     1081 ccgttgggct gaggttccaa aagtaagatg tggcgccatc aatatcatcc gaaatatatt
     1141 ctgttcccga atcggaactg caaacctgtg tttcgcccga cggggtttcg ccttcatccg
     1201 gcggagtaat gataaatata taagcattgc ctatcatatt tttatcacaa taatcggtgt
     1261 atccaacgca ggtataaatc ccctgttcgg tctggttgcc aaaagtgatg gccgaacctg
     1321 ttcccgccat aatttgtcct gtaggatcgc catccaggta aagctcataa tcgacaccat
     1381 tctgcgaccc gtcgagggta agttcaatgc catcgctgcc ttcgcaataa cccgaaccat
     1441 cggataaagt atattcagat ggagtcatgt aagccgcgca gttcagtccc tgcgaccagg
     1501 taccgttgcc acaatcgtta tctgccctga ctttgatcgt gtaggttcca aggtatcccg
     1561 gggcgagggt gaaagtggct accggatcgg ggcctgagat ggttcctgct gcatcgggtt
     1621 ccacatccca gacatacgtt gtggcccatt gcaccagcgg tgtcgaatac tgaatcccgt
     1681 cattctcctg gcaaaccgtt gtcggaccgt taggcgtctg gggctggacc ggtggggtaa
     1741 tgaccaggat gtaattggga ttcaacagtg tatcataaac ggttccgtca taaacaataa
     1801 gctggacatc atactggcct tgcgtattat aagttactac cgggttctgt gccgcggacg
     1861 ttgccgggtt tcctgcttca aaagtccagt tccaggaaat gatttctccc atcgactgat
     1921 cttcaaattc gacggctgtt gtgatacagg gtgtcacgtc ttcacaggta aatccggctg
     1981 tggcctgcat tggctccagg tcgggtatgg ctaccatatt tccctgcaaa acttctttag
     2041 tggcttcatt ctgaacgaag gctaccagtt cacagttgct ggcaacccag ctggcatcca
     2101 ttgtaaacga aagatccagg gtgacttcgt cctggctggt gaaatcgaca accgttccca
     2161 tatgggtcgg ggccatgagc ctttccacaa agttcaggtg atcctgcccc tgccaggaat
     2221 aaacgatttc cgattcggtc agcaccagat gtacggtcag attgccccag g
//
LOCUS       DAOQXS010000466         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_54590_length_2251_cov_3.957195, whole genome shotgun sequence.
ACCESSION   DAOQXS010000466 DAOQXS010000000
VERSION     DAOQXS010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54590_length_2251_cov_3.957195"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKM34_11180"
     CDS             <1..207
                     /locus_tag="PKM34_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84199.1"
                     /translation="DKANGAQIDNYKGQKELVLVPGVTQQDGCAGINAEECVIRMTEV
                     TPVRVVDPQELEFKDVKVSKVSNN"
     gene            234..1907
                     /locus_tag="PKM34_11185"
     CDS             234..1907
                     /locus_tag="PKM34_11185"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ84200.1"
                     /translation="MKKIFQSLSSRLLTIMSVLLLVGAVSPSPVKAQTSQGTDFWICF
                     PANTSSGARELYITAGAAASVNIQIPGLGYSNLVNIPAGTLATVTLPGTVDVSSNGTA
                     DNKGIHITSDNPVTVYGMNQATATTDAFLALPVDALGTDYLVMGYTRDASFVLYAQMT
                     IVATQDNTSLTITPTATGGGFTAGVPGNVVLNQGQVFQLRSNSVGADYTGSHVVSDKP
                     VAVFGGNECTNISGSLRACDHLVEQMVPTSGWGRNFLTVPLATRLAGDVFRVMAQQAG
                     TQVSVNGSVVANLNYGQFHEMILASNTYNRITSNNPILVGQYSRSSDADGVTSDPFFA
                     LVPPDEQFLNNYIISAGTGNIPNNYLNITSPTANTGTVLVDGNPVVNWTPIPSTTFSG
                     AQVSINNGIHTITSNLPIGLLVYGFGNYDSYGYIGGQAFAAVATINALDLTPTTGTAG
                     INNLRCWEAEVTDSNGDPVSGIRVDFTITGANPGAGFAFTDANGIATYCYTGTNAGQD
                     VIVATTGALADTAQFTWTDQSAPVPVSNWALFIGLFLIIATVIWKFRRA"
     gene            complement(1964..>2251)
                     /locus_tag="PKM34_11190"
     CDS             complement(1964..>2251)
                     /locus_tag="PKM34_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84201.1"
                     /translation="DVSWPEVSITPGNTYFLVFTCTNPQPMVAGNVENPYPYGMVYAN
                     PGYFPFPSFDYTFRTYTCTDMSNTIPLSNWAIYLAIALMGLAVIWVVIRRR"
BASE COUNT          560 a          600 c          565 g          526 t
ORIGIN      
        1 gataaagcta acggggcaca gattgataat tataaaggac agaaggagct tgtccttgtt
       61 ccgggtgtga cccagcagga tggttgtgcc ggtataaacg ctgaagaatg cgtgatccgg
      121 atgacggaag tcaccccggt ccgggttgtt gacccgcagg agctggaatt caaagatgtg
      181 aaggtcagca aagtatctaa taattaatcc actaattctt aaatcaatcc attatgaaaa
      241 agatattcca atccctttca agccggttgc tgaccatcat gagcgttctt cttctggttg
      301 gtgcggtctc accttctcct gtgaaagccc agaccagcca gggcacagat ttctggatct
      361 gtttccctgc aaataccagt tcaggtgcac gcgaattata catcacagcc ggagcggctg
      421 cttcggtgaa tattcagatc cccggtttag gatattccaa cctggtcaac attccggccg
      481 gtacgctggc aacggtcacc ctccccggaa ccgttgatgt atcaagcaat ggaactgccg
      541 ataacaaagg aatccatatt acatccgata acccggttac cgtttacgga atgaaccagg
      601 ccacggccac taccgatgct tttcttgccc tgccagttga tgcattgggc accgattacc
      661 tcgtgatggg ctacacccgc gatgcatcct tcgttctcta cgcccagatg acgattgtcg
      721 ctacgcagga caatacctca ctgactatta cgcctactgc aaccggcggc ggatttacgg
      781 ccggcgtgcc gggaaatgtt gtcctgaacc agggacaggt attccagctc aggtcgaatt
      841 cggtcggggc agactacacc ggctcacatg tggtatccga taagcctgtt gccgttttcg
      901 gcggaaatga gtgcaccaat atatcaggaa gtctcagagc ttgtgaccac ctggtggagc
      961 agatggtacc cacatcgggc tggggcagga acttcctgac cgttcccctg gcaacccggc
     1021 tggccggaga tgttttcagg gttatggcgc agcaggccgg aacccaggtc agtgtaaacg
     1081 gttccgttgt tgctaacctg aattacggtc agttccacga aatgatcctc gccagcaaca
     1141 cctacaaccg gatcacctcc aataatccca tcctggtagg gcaatactca cggagctcgg
     1201 atgccgacgg cgtaacctct gatccgttct ttgccctcgt accaccggat gaacagttcc
     1261 tcaacaatta cattatttca gcagggacag ggaatatccc caataactac ctcaacatta
     1321 cttctcctac agccaatacg ggaacagttc tggtcgacgg gaaccccgtt gtcaactgga
     1381 cccccatacc gagtactact ttctccggcg cccaggtgtc catcaataac ggcatccata
     1441 ccattaccag taaccttccg atcggattgc ttgtgtatgg gtttggcaat tatgattcat
     1501 acggctatat cggagggcag gcttttgcag ccgttgccac catcaatgcg ctggatctga
     1561 caccaacgac aggcacggca ggcatcaaca acctgcggtg ctgggaagcg gaggtgaccg
     1621 atagcaatgg agatccggta tccggaatca gggtcgactt cacgataacg ggtgccaatc
     1681 ccggtgcggg atttgctttt accgatgcga acggtattgc aacctattgt tatacaggta
     1741 ccaatgcagg tcaggatgtg atcgtcgcaa caacaggcgc cctggccgat acggcccagt
     1801 tcacctggac cgatcagtcg gcgccggtac cggtcagcaa ctgggccttg ttcatcggtc
     1861 ttttcctcat catcgccacg gtgatctgga aattcagaag agcataacag aattcccttc
     1921 ttaatacaca aagaggctgc atccggcgag gggcagcctt tttttatctt ctcctgatca
     1981 cgacccagat aacagccagt cccatcagcg caatggccag gtaaatggcc cagttgctca
     2041 gtggaatggt attcgacata tcagtacagg tataggtccg gaaggtgtaa tcgaaggatg
     2101 gaaacggaaa atagcccgga ttggcatata ccatgccata tggataaggg ttctcaacgt
     2161 ttccggcaac catgggctgt ggatttgtac aggtaaaaac taaaaaatat gtatttcccg
     2221 gtgtgatact tacctcaggc caggatacgt c
//
LOCUS       DAOQXS010000467         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_54782_length_2246_cov_3.444546, whole genome shotgun sequence.
ACCESSION   DAOQXS010000467 DAOQXS010000000
VERSION     DAOQXS010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54782_length_2246_cov_3.444546"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PKM34_11195"
     CDS             complement(<1..1200)
                     /locus_tag="PKM34_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84202.1"
                     /translation="MKQAFFALLVMISTCYFAPGQIPGYHSTIRGEILSYHAPQPDAT
                     TSLLVRSEDSTRFIEWESAMIAETGYPKQESGSNKPGTWNPEPGTFLLLAGIDVNPAD
                     PRAWKVFVNDRHLFTISSPLDTIQKTITWPGPDGSSLVFRTKEVDKYGDFMGYLYCNL
                     PSSMREAGKPVRFRVVGESAGSRTWFMVFEYETNDRVRLIPEQAVVKGDKGNHQVLRA
                     EIVRYAEPVEAKITVAGKKLNRKLDFGYNVLYLPVPEITTETAFDFVIKAGKTLISRE
                     SFTIAPVIPRTIYLLHHSHNDIGYTHVQPEVEEIQWQNLDRALILSDSTKDFPAGSGL
                     KWCTEVIWAFESWYEKADPAKKEQIRQALKDGRIELNALFANELVSLCGPEELDRLLE
                     AGRRIAKE"
     gene            1342..>2246
                     /locus_tag="PKM34_11200"
     CDS             1342..>2246
                     /locus_tag="PKM34_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84203.1"
                     /translation="MILRISDRPALCKLRCRFRDAWRLIPVFVLLALLSLSGRLASQP
                     LLVEDFDYTNGTAITANGWTAHSAGGTNAILVTNPSTTCSYPGYLSSGVGGEVTFVAS
                     GEDDNRAFSAQTSGTIYFGFLVNISAATTGGDYFFHFGATTLGTNYRGRVFAKRDASN
                     NLAFGISNSTSTATYSGFVYALNTTYLIVLKHEIVSGTGNDVSSIYINPPLNAPIPAT
                     GWLVAADASGDLADVGRVAIRQAAPAGKLDGIRVSTTWSDIVGAPNTPTLSVDPSTLS
                     GLHYVEGQGPSTSLSYLLSGINLTPA"
BASE COUNT          521 a          553 c          567 g          605 t
ORIGIN      
        1 ctctttcgca atccggcgcc cggcctccag cagcctgtcg agctcttccg gcccacagag
       61 cgacaccagt tcgttggcaa ataatgcatt cagctcaatc cgcccgtctt tcaatgcttg
      121 cctgatctgt tcttttttgg ccggatcggc tttctcatac cagctctcaa aggcccatat
      181 cacttcggtg caccatttga gacctgaccc tgccggaaaa tcttttgtcg aatcgctcag
      241 aatcagcgcc cggtcaagat tctgccattg gatctcctcc acctccggct gaacatgcgt
      301 gtagccaatg tcgttgtgcg aatgatggag cagatagatc gtccgcggga taacgggtgc
      361 gatggtaaag gattccctgg agatgagtgt tttaccagcc ttgatgacaa aatcgaaggc
      421 tgtttcggtc gttatctcgg ggacagggag gtagagaaca ttgtaaccga aatcgagctt
      481 tctgttcagc tttttgccgg caacagtgat ctttgcttcc accggctccg catacctgac
      541 gatttcggcc cgcaatacct ggtggttgcc tttgtcgcct ttcacaacag cctgttcggg
      601 gatcagcctc acccggtcat tcgtctcata ttcaaaaacc ataaaccagg tacggctgcc
      661 tgcactttca ccgacgaccc tgaaccgcac cggttttcct gcttcccgca ttgaagaagg
      721 taagttgcag tagagatagc ccatgaaatc gccgtattta tcgacctctt ttgttctgaa
      781 aaccaggctg gagccgtccg gtccgggcca ggtaatagtt ttttggatgg tatcaagggg
      841 tgaagatatc gtaaacagat gcctgtcgtt cacaaacact ttccaggcgc ggggatcggc
      901 ggggttgacg tcgatgccgg cgaggagcag aaaagttcca ggttccgggt tccaggttcc
      961 aggtttatta cttccagatt cctgtttcgg gtatccggtt tccgcaatca tagcggattc
     1021 ccattcgatg aagcgggtgc tgtcttcgga gcggaccaac agcgaggtgg tggcatcggg
     1081 ttgcggggcg tggtaggaga ggatttcgcc ccggatcgtt gaatggtagc cggggatttg
     1141 gcccggggca aaatagcagg tggaaatcat aaccaggaga gcgaaaaaag cttgcttcat
     1201 ctcaatatta attttttatg aataatgtaa aaatagccgg atttgctaac taatatatta
     1261 ttattttagc gaactgatta aatttgcgct taaactcaaa attgcaatat taaaccaaat
     1321 ctttacttaa aattttccat tatgatatta agaatttctg acaggccggc gctgtgcaag
     1381 ctgaggtgcc gtttccgcga cgcatggcgt cttatccctg tttttgtgtt gcttgcactg
     1441 ttatcccttt cgggtcgcct ggcaagtcag cctttgctgg tagaggattt tgattatacc
     1501 aacggaaccg ccatcacagc caatggctgg acggcacata gcgccggcgg aaccaatgca
     1561 atcctagtga cgaatccttc aacaacctgc agttatcccg gctacctttc gtcgggcgta
     1621 ggaggcgaag tgacttttgt agcatccggt gaagatgaca accgggcatt tagtgcgcaa
     1681 acttccggta ccatttattt tgggttttta gttaatatat cagctgccac cacaggcggt
     1741 gactatttct tccattttgg tgccactact ttgggaacaa attaccgggg ccgggtattt
     1801 gcaaagagag atgccagtaa caatcttgcg ttcgggattt caaacagcac aagcacagcg
     1861 acttacagtg gatttgtata tgctttgaat accacatatc tgattgtact gaaacatgaa
     1921 attgtaagtg gaaccggtaa tgatgtttcc tcaatttata ttaatccgcc tcttaatgca
     1981 ccgattccgg caacgggttg gttggtggca gccgatgctt ccggcgacct tgcagatgtt
     2041 ggcagagtgg ccattcgtca ggcagcaccg gctgggaagc tggatggcat ccgcgtttcc
     2101 accacatggt ccgacattgt cggagctcca aatacaccga ccctcagtgt ggatccgagt
     2161 actttgagcg gattacatta tgtggaagga cagggccctt cgacctcact gtcgtatttg
     2221 ctaagcggaa ttaaccttac tcctgc
//
LOCUS       DAOQXS010000468         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_54822_length_2245_cov_3.703653, whole genome shotgun sequence.
ACCESSION   DAOQXS010000468 DAOQXS010000000
VERSION     DAOQXS010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54822_length_2245_cov_3.703653"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKM34_11205"
     CDS             <1..370
                     /locus_tag="PKM34_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84204.1"
                     /translation="AFGETIRGNEKIYHWLLKNGYPELAALSSAIRGSQEAVQWLLKN
                     HYPHLAALDAAIDESPKAYEWLSKNNYQLLMVFADACRDKPEAVAWLRKNELDIFVHL
                     AIIIKKWRGEQNFDYHKIHF"
     gene            complement(400..1086)
                     /locus_tag="PKM34_11210"
     CDS             complement(400..1086)
                     /locus_tag="PKM34_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ84205.1"
                     /translation="MEQYRILLVDDEPDILEFLGYNLRREGFEVFTSSNGRDGLAKVK
                     EHKPHLVVLDVMMPEMDGIETCREIRQIPELRNTLVIFLTARGEDYSQIAGFDAGADD
                     YVTKPIKPRVLVSRVKALLRRYKEEAADSGPDQFISTDEFVIDREKYVVIKEGKEFHL
                     PRKEFELLSLLVSRPNKVFTREEILSGIWGDDVIVGDRTIDVHVRKIRERLGTENIRT
                     IKGVGYKYEN"
     gene            1262..1891
                     /locus_tag="PKM34_11215"
     CDS             1262..1891
                     /locus_tag="PKM34_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNQ84206.1"
                     /translation="MRQLRYIIFTLSFFLMGSAVQSQVFNGGVMAGVAGTQVSGDDYS
                     GFHKAGLFLGGFVNLQFSEHSALQMELEFFQKGSRENPDSTNNYQQYLFRLNYVELPV
                     LYQYTAGKRFKFEAGPSLGFLINYYEENNYYEIKGGNPGARVTFQINAGMYVYITRNL
                     LVNIRTNNSLINIRADNATGDVLRIFPGNYGQFNDCLVLSLFYQFDPAW"
     gene            complement(1899..>2245)
                     /locus_tag="PKM34_11220"
     CDS             complement(1899..>2245)
                     /locus_tag="PKM34_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84207.1"
                     /translation="STGETTQAIEVSLAGTYSVSVTNYCGNGYDEIEVDVSTGLSEPT
                     MEDIQIAVSGNQVILTHLPPDIMAIQMITLDGRTVFDGKSIEKITLGIPGIYIIRIIS
                     SEKIITRKVFIH"
BASE COUNT          569 a          537 c          520 g          619 t
ORIGIN      
        1 ggccttcggc gagacgatca ggggtaacga aaagatttac cactggctgc tcaagaacgg
       61 gtacccggag ctggccgcgc tttcaagcgc tatccgggga tcgcaggagg cagtgcaatg
      121 gttgctcaaa aaccattacc cgcacctggc cgccctcgat gcggctatcg acgagtcgcc
      181 gaaagcctat gaatggctga gcaaaaacaa ttatcagcta ttgatggttt tcgccgacgc
      241 ctgccgcgat aaaccggaag cggtggcctg gctgagaaaa aatgagctgg atatctttgt
      301 ccacctggct attatcatta aaaaatggcg tggtgagcag aattttgatt accacaagat
      361 acatttttag ttccaggttc ttaagttcca ggttccagat tagttttcgt atttgtagcc
      421 gacgcctttg atcgtccgga tgttttctgt tcccagccgt tccctgatct tcctgacatg
      481 tacgtcgatc gtccggtcgc cgacgatgac atcatcgccc cagatacccg aaaggatctc
      541 ttcacgggtg aacaccttgt tcggcctgga aaccagcagg ctcaacaatt caaattcctt
      601 cctggggaga tgaaattctt tgccttcttt gatcacgaca tatttctcac ggtcgatcac
      661 aaattcatcg gtactgataa actggtccgg gcctgaatca gcagcctctt ccttataacg
      721 ccggagaagt gccttgacgc ggcttaccaa aacacggggc ttgatcggtt tggtcacata
      781 gtcatccgct ccggcatcga aaccggcaat ctgtgaataa tcttcaccac gggctgtgag
      841 aaaaataacc agcgtattcc gcagctccgg gatctgcctg atctcgcggc aggtctcaat
      901 tccgtccatc tcgggcatca tgacatcgag cacgaccaga tggggcttgt gttcttttac
      961 cttcgccaat ccgtccctgc cgttactgct ggtaaatact tcaaatcctt cccggcgaag
     1021 gttatacccg agaaactcca ggatatccgg ttcgtcatcc actaataaaa ttctgtattg
     1081 ctccataaag gaaattgatc tggttatgat tataattggt taaacccttc tgcaacagtc
     1141 attaataaaa gacattcggc agcgaaatta ctattttatc caggagcaga cgcctttatc
     1201 gggtctaaca gcttaaaatt acgatatttg ccgtttggtt tatgattaat tggcagttat
     1261 aatgaggcaa ttgagatata ttatttttac gttaagtttt tttctgatgg gttcggctgt
     1321 acaaagccag gtattcaacg gtggagttat ggcaggagtg gcgggaacac aggtttccgg
     1381 agatgattat tccgggttcc ataaagcggg cctttttctc ggcggatttg tcaacctgca
     1441 gttttcggaa cactccgcac tgcagatgga gcttgagttt ttccagaaag ggagccgcga
     1501 aaatccagac tctaccaata attaccagca atatttattc cggctcaatt atgtggaatt
     1561 gcctgtttta taccagtata cggcaggtaa gcggtttaaa ttcgaagcgg ggccttcgct
     1621 cgggttcctg atcaattatt atgaagagaa taattattat gagataaaag ggggaaaccc
     1681 cggcgccagg gtcacattcc agatcaatgc cggaatgtat gtttatatca cccgaaacct
     1741 gctggtcaat atccggacga acaactcgct cataaatatc cgtgccgata atgcaacggg
     1801 agatgttttg cggattttcc ccgggaatta cgggcaattc aacgattgcc ttgtactcag
     1861 cctgttttac cagttcgatc ccgcctggta actcctgttc aatgtatgaa tactttccgg
     1921 gttattattt tttcagatga tataatgcgg ataatgtata ttcccgggat cccaagagta
     1981 attttttcaa tggacttgcc atcgaaaacc gttctaccat ccaaagtaat catctgaata
     2041 gccatgatat ccggtggtaa atgggtcagg ataacctggt ttccggaaac ggctatctga
     2101 atatcttcca tcgtgggttc cgataggccg gttgaaacgt caacttctat ttcatcgtag
     2161 ccatttccgc aataattggt caccgaaacc gagtaagtcc cggccagact gacctcgatg
     2221 gcctgagtgg tttcacccgt cgacc
//
LOCUS       DAOQXS010000469         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_55482_length_2228_cov_3.961804, whole genome shotgun sequence.
ACCESSION   DAOQXS010000469 DAOQXS010000000
VERSION     DAOQXS010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55482_length_2228_cov_3.961804"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1460
                     /locus_tag="PKM34_11225"
     CDS             177..1460
                     /locus_tag="PKM34_11225"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84208.1"
                     /translation="MKKILVLITAVISISGISQGQLTIIQYPSPEEYCYAAGTEFITE
                     TEGFLVMVWDDGIPTPGGGCYAQYYLTYDGGQNWEFLSQSSFEAKNGSIRFIEDNEEG
                     EYYFGYIAGDYSVVGSYNTVSGSFNGYLSTPSAYLIDGGIGMDGSYYYIIRYWAKGIR
                     YGLWKVMDNGGSEELFSSTDSLLLQEPFFTSSSEGFIRFNLSETGPDGILHTIDSGAT
                     FDILYTNDSLTFTNMVFRNDEEGYVTTLQGYLLKTIDGGNNWETLPLLPGTGLYSITF
                     LDNLTGYCGGNNGTIFKTIDEGVTWQQQIMPELINIRKIMMSGESKGFLQAEFYTYLL
                     DVSSGISESSNHYGIFPNPATDKITVRSGLFPGESDIEVELFDLTGEKVEKTLNLPAS
                     DQSAVDVSDLPSGFYLIRLTTGDKLLYSGKVLILR"
     gene            complement(1522..>2228)
                     /locus_tag="PKM34_11230"
     CDS             complement(1522..>2228)
                     /locus_tag="PKM34_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84209.1"
                     /translation="IQDNAETKDTINVKVLNFREAGYELVGVELMLDSILHPRISDLE
                     ISLTHDGITAVPVSHPAGPGANILWMRLKDDATKQIADGAAPYSGDYKPQTLLGIFNG
                     LDPDGEWILTIGDNQAGSTGMLKAWGVKPLFDRKVGIEDPVTPGKPDEIILLQNVPNP
                     FSGITRISWTSEVAGRTILKIYDNQGRELETLVDEFKPKGEYSVEFNGSHFSPGVYCY
                     RLQVGDVVLTRKCIIK"
BASE COUNT          618 a          462 c          488 g          660 t
ORIGIN      
        1 catgataacc tccttcttat ttttttaaaa aatatcaaaa ttaatataaa tattgacgtt
       61 ataacatcat tatttagaag atccttcagc aaaatgcttc aatgacaacc aaaaaaaatg
      121 ttattctttt cactttaaac cgcttatctt cttaaattta cacaaattct tgtgtgatga
      181 aaaaaatctt ggtactgatc acagctgtca tttctatatc aggtataagc cagggacagc
      241 tcacaattat tcaatatcct tcccctgaag agtattgcta tgcagctgga acagaattca
      301 taacggagac agaaggtttt ctggttatgg tttgggatga tggaatacca actccaggtg
      361 gagggtgtta tgctcaatat tatctgacat atgatggagg tcagaattgg gaattccttt
      421 cccagtcatc ttttgaagct aaaaacggtt cgatcagatt tatagaggat aatgaagaag
      481 gagaatatta ttttggttat atagcaggtg attattccgt cgttggaagc tacaatacgg
      541 taagcggctc atttaatgga tatttatcca ccccatcagc atatcttatt gatggcggaa
      601 ttggaatgga cggatcatat tattacatta tcagatactg ggcaaaaggg atcaggtacg
      661 ggctatggaa agtgatggat aatggaggat cagaggaatt attttcttca acagactccc
      721 tgctcctgca ggaacctttt tttacgagct cctcggaagg atttattcgt ttcaatttat
      781 cagaaacggg ccctgatggg atcttacaca cgatcgacag cggtgcaacc ttcgatattt
      841 tgtataccaa tgattcactg acattcacca atatggtatt tcgcaatgat gaggaaggat
      901 atgttacaac tttacaaggt tatttgttaa aaactatcga tggaggaaat aactgggaaa
      961 ccctccccct tttgcccggt acaggacttt atagcattac ctttctggac aacttgaccg
     1021 ggtattgtgg tggaaataat gggacgattt ttaaaacaat tgatgaagga gttacatggc
     1081 agcaacagat tatgccagaa ttgatcaata tcaggaagat tatgatgtcc ggtgaatcaa
     1141 aaggatttct tcaggcagaa ttttacactt atttgcttga tgtttcttca ggaatttcag
     1201 agagctcaaa tcattatggc atttttccaa atccagcaac cgacaaaatt acagtccgtt
     1261 ccgggttgtt tcctggtgaa agtgatatcg aagtcgaact ttttgactta accggagaaa
     1321 aagttgaaaa aacactcaac ttgccagcca gtgaccagtc tgcagttgat gtttcggact
     1381 tgccatccgg gttttatttg ataagactga ccaccgggga taaattgctt tattcaggta
     1441 aagttctgat acttagataa agtcgttcta atcccgacat tatccattta attattgatg
     1501 cttattgcaa ttgaaaagca attacttgat gatgcatttc cttgtaagca ctacatcccc
     1561 gacctgcaac cggtaacaat acacgcccgg gctaaagtgc gaaccattaa attccacgga
     1621 atattcacct tttggtttga attcatccac tagcgtttcc agctccctgc cctggttgtc
     1681 atagatcttc aggatggtcc ggcccgcaac ctcactggtc cagcttatgc gtgttatacc
     1741 ggaaaatgga ttgggaacat tctgtaagag gatgatttcg tccggtttgc ccggggtgac
     1801 ggggtcttcg atacctactt tcctgtcgaa cagcggtttc acgccccagg ctttcagcat
     1861 cccggtactt ccggcctggt tgtcgccgat cgtcaggatc cattcgccgt cgggatcaag
     1921 gccattgaaa atacccagca gtgtttgggg cttgtaatcg ccggagtaag gtgcggcacc
     1981 gtcggcgatc tgttttgtcg cgtcgtcttt cagccgcatc cagaggatat tggccccggg
     2041 accggccgga tggcttacag gaacggccgt aatcccatca tgcgtgaggc tgatctccag
     2101 gtcgctgatc cgggggtgaa gaatggaatc gagcataagc tccacgccaa ccagttcgta
     2161 accggcttcc ctgaaattca ggacctttac gttgatcgta tcttttgtct cagcattatc
     2221 ctggatgg
//
LOCUS       DAOQXS010000470         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_56057_length_2213_cov_3.798424, whole genome shotgun sequence.
ACCESSION   DAOQXS010000470 DAOQXS010000000
VERSION     DAOQXS010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56057_length_2213_cov_3.798424"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKM34_11235"
     CDS             <1..1057
                     /locus_tag="PKM34_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84210.1"
                     /translation="VNPHLQEVPASALSSSYSDSPFQTDTETESVTESVTETETIKQN
                     EQAVLIPENLHQEKFAVEADGPDVVQEPVDHEITGLPMTPVMSGYDTLQTHIDIDSTV
                     YTGNDSTPDRTDVYVEKPGRNEYFLVGLTTTPELMFNTLEGDKFINNFGVEAAFGYGK
                     ISVHTGIGVSISKGIVENEIEYNSYLGTYNRLDSISFTFSENSGDFIPELFMSTGEAW
                     DDDASYDSLEIVKRYTYLQVPLTLGFDFLQKEKFSAGVRIGTTMSVLLDSKQLTGNYE
                     PGRNIVIGTSRLTPDRVKTTWLATAGISGTAMLSRKIYLEIEPQARYYYGSVYEDSGK
                     LKKPWSVGVRLAIWYKF"
     gene            1211..>2213
                     /locus_tag="PKM34_11240"
     CDS             1211..>2213
                     /locus_tag="PKM34_11240"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ84211.1"
                     /translation="MKNIIILLIVVFPGITGFSQGWVSISGTVYDVDNGMPIPGQSVT
                     IMSDSANGTFYYNVVVTDSLGHYFDLLPVAPGTVNSITVQVTDCNGLIMSFAIWINPN
                     TSQYIQDFYICTATVICQAWFTFRPEPEGSATSYRFLDASTGNGYTTFWSFGDGTFSE
                     EQNPLHAFPGPGTYEICLTIQGTSCQDAFCMSLTISDTVYRQIYGQVFAGNLPVQSCE
                     VYLYSQAPAGGGYTLLEEGFPVDSNGVFYFTLVPEGVYLLQAVPAGSDSYLPAYFGDA
                     ISWQQAAPIIIGEPENPYNIHLEEIGVGNALIGPGSILGQVNTVGMERSGVGLINILL
                     "
BASE COUNT          569 a          487 c          569 g          588 t
ORIGIN      
        1 ggtaaatcct catttacaag aagttcctgc ttctgcttta tcatcatctt attccgattc
       61 ccctttccag actgataccg aaaccgaaag tgtaaccgaa agtgtaaccg aaaccgaaac
      121 gataaaacaa aatgaacaag ctgtcctgat ccctgaaaac ctgcaccagg aaaaatttgc
      181 ggttgaggcg gatggacccg acgtggttca ggaacctgtt gaccatgaga ttactggctt
      241 gccaatgact cctgtgatga gtggttatga caccttgcag acacacattg acattgattc
      301 gaccgtttac accggcaatg attctacccc ggaccgaacg gacgtgtatg ttgaaaaacc
      361 cggtcgcaat gaatatttcc tggttggatt gacaaccacg cctgagctga tgttcaatac
      421 gcttgaaggc gacaaattta tcaacaattt cggtgtcgaa gcggcgttcg gatatggaaa
      481 gatctctgtt catacaggga tcggtgtttc aatatcaaaa ggaatcgtgg agaacgagat
      541 cgaatacaac agctatctcg gcacctacaa ccggctcgat tcgatcagtt ttacctttag
      601 tgaaaacagc ggcgatttta ttccggagct tttcatgagc accggggaag cctgggatga
      661 cgatgccagc tatgattcgc tggaaatcgt gaaacgttac acctatttgc aggtgccact
      721 gacgttggga ttcgatttcc tgcaaaagga gaagttctcg gcaggcgtaa ggataggtac
      781 cactatgtcg gtattgctgg attcgaaaca actgacgggc aactatgaac ccggtcggaa
      841 catcgtgatc gggaccagcc ggctgacccc cgacagggtt aaaacgacat ggctggctac
      901 tgccggcatc agcggaacag cgatgctgtc acgcaaaata tacctggaga ttgagcccca
      961 ggcgaggtat tattacggat cggtctatga agattcaggc aagctgaaaa aaccatggtc
     1021 agtaggggtg aggctggcaa tttggtataa attttaaatg gtcataaggt tgttataagg
     1081 tggtcataag gtggtcatat ggtggtcata aggtggtgat aaggttgtca taaggttgtt
     1141 ataaggtggt cataaagtgg tcataaggtg gaatttggaa tttggaattt ggaattttaa
     1201 atagtgtgtc atgaaaaata taataattct actgatagta gttttccctg gtattactgg
     1261 tttttctcag ggatgggtat ccatcagcgg aacggtgtac gacgttgaca acgggatgcc
     1321 gataccgggc cagagtgtga ccatcatgtc ggactctgca aacggaactt tctattacaa
     1381 tgtcgtggtt accgattcat tgggccatta cttcgatctt ctgccggtcg ctccgggaac
     1441 cgtcaattca attactgtcc aggttacgga ttgtaacggt ctgataatga gtttcgccat
     1501 atggataaat ccaaatacca gtcaatacat acaagacttt tatatatgca ccgcgacggt
     1561 catctgccag gcctggttta catttcgacc cgaacccgag ggttcggcaa cctcctaccg
     1621 ttttcttgac gcttcaaccg ggaacggtta tacgacattc tggagttttg gagacggaac
     1681 gttttctgag gagcagaacc cgctgcatgc ttttccgggt ccgggtacat atgagatctg
     1741 cctgacaatt caggggacgt catgccagga tgctttttgt atgagcctga cgatcagcga
     1801 taccgtttac cggcagatat acgggcaggt ttttgccggg aatctgcctg tgcagtcctg
     1861 tgaggtttat ctttattcac aagcccctgc cggtggaggg tacacgctgc tggaagaagg
     1921 ctttccggtc gattcaaacg gcgtctttta ttttacgctg gttccggaag gcgtttacct
     1981 gttgcaggct gttccggccg gatccgattc atacctgcct gcatatttcg gagatgccat
     2041 ctcatggcaa caggctgcgc ccatcatcat tggcgaaccg gagaatccgt ataatattca
     2101 tcttgaggaa atcggggtag gcaatgcttt aatcggcccg ggatctatcc tcggtcaggt
     2161 caatacagtc ggaatggaac gttcgggtgt cggcctgatc aatattctcc tat
//
LOCUS       DAOQXS010000471         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_58131_length_2163_cov_3.045066, whole genome shotgun sequence.
ACCESSION   DAOQXS010000471 DAOQXS010000000
VERSION     DAOQXS010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58131_length_2163_cov_3.045066"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PKM34_11245"
     CDS             complement(<1..1119)
                     /locus_tag="PKM34_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84212.1"
                     /translation="MAKIKIFQIFLLFTIHFSLFIIPCSLLADKLDSLMRVHDTSTVV
                     YFFHRTDSLAMGRFYTVESNRLTGFQRYNPLDQQGRFSASSSNTGLPYKNLIFRPLLS
                     GGFFTGMESYDAFTYSSENTRYYRHLIPVTFLQYSSGPEKEQLFRVVHSHTIKRLVTL
                     GVDFNLINSPGHFINQKSDDKSVVFTGQYYTKDLRFGAIANYRHNKFISGENGGIVND
                     TVFEEQLETDTKLFDVNLTTARNLVKESGFMANTYFYLSGKPEIIDSTKMRPPTFHAG
                     RIDYTFNYTRAIQIYSDQDPLADFYQPFGPVIDSTETFDSLMINQYENRFSWSNLRIG
                     DVPEKKYLTIIFAAENKIATIADSVSDRRFSSWIPEASM"
     gene            complement(1287..>2163)
                     /locus_tag="PKM34_11250"
     CDS             complement(1287..>2163)
                     /locus_tag="PKM34_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ84213.1"
                     /translation="INFEVKAGERVGIIGKNGTGKTTFLDLLSGKIRPQKGRVEPGET
                     VKFGYYRQEGLSFKEDDRVIDVVRRVADEVKTASGDWMSAAAFLNYFLFPYHTHNQFV
                     YKLSGGEKRRLYLVTVLMQHPNFLILDEPTNDLDILTLNVLEEYLVNFNGCVMVVTHD
                     RFFLDNIVDHLFVFEGNAKVKDYPGNYSQWKEWAEKQELKNKAIQKAKEPVTERPQRE
                     KKPGLTFKEKRELEQLEKDLELLEEEKYTIESSFNDSNLSPDHLIKNSERLGEIIKEI
                     DVKSERWIELSDLAI"
BASE COUNT          546 a          508 c          491 g          618 t
ORIGIN      
        1 catcgaggct tccggtatcc agctggaaaa tctccggtcg gaaacggagt cggcaatggt
       61 ggctatttta ttttcggcag caaatatgat cgtcaggtat tttttctcag gcacatcgcc
      121 aatccgcaga tttgaccagg agaaccggtt ttcgtactga ttgatcatca gcgaatcgaa
      181 agtctccgtc gaatcgatca ccggcccgaa cggctggtag aagtcggcaa gggggtcctg
      241 gtcgctgtaa atctgaatgg cacgggtata attgaaagtg taatcgatcc ttccggcatg
      301 gaaagtcggc ggcctcattt ttgtcgaatc gatgatctcc ggcttgcccg acaggtagaa
      361 ataggtgttc gccataaaac ccgactcttt aacaagattc cgggctgtag tgagattgac
      421 atcgaaaagt ttggtgtcgg tttccagttg ctcttcaaaa accgtatcat tgacaattcc
      481 tccgttttcc ccggaaataa atttgttatg ccgataattt gcgatcgccc cgaatcgcag
      541 gtccttcgta taatattgcc ctgtaaagac aacgctttta tcgtccgatt tctggttgat
      601 aaagtggccc ggagagttga tcaggttaaa atccacaccc agggttacca gccgttttat
      661 ggtgtggcta tggactaccc ggaacagctg ctctttttcc gggccgctgg agtactgcag
      721 aaaggtgacc gggatcaggt gcctgtagta ccgcgtattt tcgcttgagt atgtgaacgc
      781 atcgtatgat tccatcccgg taaagaatcc tccggaaagg agaggtctga atatcagatt
      841 tttataagga aggcccgtgt tggatgaact ggcgctgaac ctgccttgct ggtcgagcgg
      901 gttatagcgc tgaaaacccg tcagacggtt gctttcaacg gtataaaagc ggcccatggc
      961 caggctgtcg gtacgatgaa aaaaataaac aacagtagat gtgtcatgaa cccgcatcag
     1021 cgagtcgagt ttgtcggcaa gcagggaaca gggaataatg aatagtgaaa agtgaatagt
     1081 gaataacaga aagatctgaa atattttaat tttagccatt ggatcgggtt gccgactttt
     1141 ttaacggttc gcgtgatgtt ttatttttca aaaatacgca aatcctaatt gatcaaccac
     1201 aaaggcgcac aaagtaatga tttgtctatt gaccggcctt tgtgtgcctt cgtgaaatcc
     1261 ttggtgtgcc tttgtcgtta ttatttttaa attgccagat cactcaactc tatccaacgc
     1321 tccgatttaa cgtcaatctc tttaatgatc tctccaagcc gttctgaatt tttaatgaga
     1381 tgatccggtg aaaggttgct gtcattgaat gaggattcta tggtatattt ctcttcttca
     1441 agcaattcca gatccttctc cagttgctcc agctcccgtt tctccttaaa ggtcagtccc
     1501 ggcttcttct cccgttgcgg tctctccgtt accggttctt ttgccttctg gatagcttta
     1561 ttctttaatt cctgcttttc agcccattct ttccattgcg aataattccc cggataatct
     1621 ttaaccttcg catttccttc aaaaacaaag agatgatcga caatattatc caggaaaaaa
     1681 cggtcgtggg tgacaaccat cacacacccg ttaaaattga caagatattc ttccagaaca
     1741 ttaagggtaa gaatatccag gtcgttggtc ggttcatcga ggataaggaa attcggatgc
     1801 tgcatcaaaa cggttaccag atataatctt cgtttttctc ctccactaag cttatacaca
     1861 aactgattat gagtatgata ggggaataga aaataattga ggaaggctgc ggccgacatc
     1921 caatcgccgc tggccgtctt cacctcgtcg gcaacccggc gcaccacatc gatcacccgg
     1981 tcatcttctt tgaacgatag cccttcctgc cggtagtagc cgaatttgac cgtttcgccc
     2041 ggttccaccc gccctttctg cggcctgatc tttcccgata agaggtccag gaaagtggtc
     2101 ttgccggttc cgtttttacc gattataccg acacgctcac cggcctttac ctcaaaatta
     2161 ata
//
LOCUS       DAOQXS010000472         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_59039_length_2143_cov_3.195402, whole genome shotgun sequence.
ACCESSION   DAOQXS010000472 DAOQXS010000000
VERSION     DAOQXS010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59039_length_2143_cov_3.195402"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2143
                     /locus_tag="PKM34_11255"
     CDS             <1..>2143
                     /locus_tag="PKM34_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84214.1"
                     /translation="CVGTTVKFKPNFIPEPDSIRWFFDEFEPGNISTELYPEYTFLHA
                     GIHEIVVDVWYPSGRYEHTSRELEIFPNPEPDLGPDTSICEGNPLVLNSNCSADQYSW
                     STSQFGVSSITISDSGTYWVRAKFLEGGCEAYDTIHVGFHPPIIIDETTLIITPTTCT
                     GASGSITGVNAQGSAPFAFQWYDLSGNPFGTNINATNLPAGQYQLTIIDGNSCQAVSD
                     VYTIEDAGNLEVLNVELTQPHCGRPDGQIVVHAFSPSGSTLEYSINDGADYSADSVFT
                     GLFDGNYVVRIRDINGCEGFYFENPVSLEDIPGPQITQVTVTDETDFLGNGSIEIVAS
                     GSTPVIYYSIDSGATWQINEGNFYNLISITYYIIIKDDNGCDTSFTVEVQNVILTWLH
                     AVTSEGGHCLGNTALVPVNVDNFNSVADFRLKLGYNAGNLECEGFANVQPQLADSLSG
                     WIDPVAGDIHLVWKSPDAVTFAGTEKVTDLVFRAKNPGQGELSWYTGETESYFTNAGG
                     NPIPAQFSTGEVSIYEPPEIFLAQSKTVCPGDTVFIMSIAAGNQPPIDYHWIYPDGDT
                     SNSDPFFLSVTQSDAGFYTLLATDRVGCTDQKSIELIVSENPVAAFYGTDTLEMHTGD
                     LLDAGTGLSSYRWNTGDTTESIVINAEGMYSVEMESPVGCLGSDSVYIKLTTEEIPET
                     HLLIPNAFTPDGDGVNDTFQVFYNGNDLSQFT"
BASE COUNT          565 a          500 c          504 g          574 t
ORIGIN      
        1 tgcgtaggca caacggtaaa atttaaaccg aatttcatcc ctgaaccgga cagtatccgt
       61 tggtttttcg atgaatttga gccgggaaat atatccaccg aactatatcc tgaatacaca
      121 tttcttcatg ccggcattca tgaaattgtc gtagatgtat ggtatccctc tggccggtat
      181 gaacatacat cccgggagct cgagatcttt ccaaatccgg aacctgattt aggaccggat
      241 acttccattt gtgaaggcaa tcctctggtt ttgaattcaa attgctcggc agatcaatat
      301 tcatggtcca ccagccagtt tggcgtttca tccattacta tttctgattc cggaacatat
      361 tgggtaaggg caaaattttt agaaggtggt tgtgaagcat atgatacgat ccacgtagga
      421 tttcatccgc ccattattat cgatgaaaca actcttatca taacacccac cacctgtaca
      481 ggcgcaagcg gatccatcac tggggtcaac gctcaaggct ctgcaccctt tgcatttcaa
      541 tggtacgatt tatccggcaa tcccttcggc acaaacatca atgcaacgaa cctgccagct
      601 ggacagtacc aactcactat catcgatggc aatagctgcc aagcagtttc agatgtgtac
      661 actatcgaag atgcaggaaa tttggaggtt ttgaatgtcg aactcaccca accgcactgt
      721 ggacgacctg atggacaaat cgttgtgcat gctttttctc cttcaggatc aacacttgaa
      781 tattctatca atgatggagc ggactattcg gcagatagcg ttttcaccgg attatttgat
      841 ggaaattatg ttgtaagaat aagagatatc aatggttgtg aaggatttta ttttgaaaat
      901 ccggtaagtc ttgaagatat tcccggcccg cagataacgc aggttactgt gacggatgaa
      961 acggattttc tgggaaacgg atcgattgag atcgttgcca gtggctcgac gccggtaatt
     1021 tattattcga ttgatagcgg ggcgacgtgg caaatcaatg aagggaattt ttataactta
     1081 ataagtatta catactacat aataattaaa gacgacaacg gctgcgacac gagttttacc
     1141 gttgaagttc agaatgttat tctcacttgg ctgcatgctg ttacaagcga aggcggtcat
     1201 tgtttgggta atactgcgct ggtgccagtg aatgtcgata atttcaacag tgttgctgat
     1261 tttcgcctga agctgggcta taatgctggt aaccttgaat gtgagggttt tgccaatgtt
     1321 cagccgcagc ttgccgacag cctctccggt tggatagatc cggtggccgg cgatattcac
     1381 ctagtctgga aatcgcctga tgcagttaca tttgcaggaa cagaaaaagt caccgacctg
     1441 gtgttcaggg cgaaaaatcc cggacaagga gagctttcat ggtacaccgg ggagactgaa
     1501 agctatttca ccaacgccgg cggcaatccc atcccggcgc agttcagcac aggcgaagtg
     1561 agtatctacg agccgccgga gatcttcctg gcccagagca agaccgtttg ccctggagat
     1621 accgttttca tcatgagcat cgccgccggc aaccagccgc ctatcgatta ccattggatc
     1681 taccccgacg gcgatacttc taatagcgat ccttttttct tatccgtcac acagtctgac
     1741 gctggctttt acactcttct cgccaccgac cgtgtcggct gtaccgacca gaaaagcatc
     1801 gagctgatcg tcagcgagaa tccggttgca gctttctatg gcaccgatac gctggaaatg
     1861 cacacgggcg atttgctgga tgcaggaaca ggcttatctt cctatcgctg gaataccggc
     1921 gataccacgg aaagtattgt tatcaacgcc gaaggaatgt actcggttga aatggagtca
     1981 ccggtgggtt gtttgggttc ggattccgtt tatataaagc tgactactga agaaataccc
     2041 gaaacccacc tgttaatccc caatgccttt acgcccgatg gagatggcgt gaatgatacc
     2101 tttcaggtat tttacaatgg taatgacctg tcacaattca cca
//
LOCUS       DAOQXS010000473         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_60062_length_2120_cov_3.838257, whole genome shotgun sequence.
ACCESSION   DAOQXS010000473 DAOQXS010000000
VERSION     DAOQXS010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60062_length_2120_cov_3.838257"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1227)
                     /locus_tag="PKM34_11260"
     CDS             complement(7..1227)
                     /locus_tag="PKM34_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84215.1"
                     /translation="MLPLKPFAQYTDNFESYTVGGYIAAQNPTWWTTWSNLPGSAEDG
                     QISTDFASSPTKSVLVDLNPGATDLVLKLGNKTSGVWEVNFDYYVQTGYSGYYNIQHN
                     ETPGIQWAFEIYFHTDGTGNLYAGSATPITFTYPKNTWFPIVNHIDLNADLITLYING
                     TAIYTWPFHYTSYSTTGINQLGGVDFFAGAEGTDIPKAYFDDVEFINVVPPDNFVEIG
                     TGTGYGSYPAYYGPWGNYWENCHTQQLYLASELGSPTGKKFTKLAWNFQQIPTGNKYL
                     NNVIILIKETTATSLTAGAYADMTGATQVFSASSYTPATATGWQILDIADFEWTGTSN
                     LIVDILWGDNGYYTSPYFRTYKTAGSGTRTLIGYADSETPPNYDGATTDYDNMRWYWE
                     PLLPPGSTQQKGHL"
     gene            complement(1435..>2120)
                     /locus_tag="PKM34_11265"
     CDS             complement(1435..>2120)
                     /locus_tag="PKM34_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84216.1"
                     /translation="YGNMMNFGGWQTLLQINPELDYNWNITGHLQTVTGAVMPMSKTV
                     TPSAINGQSLATSPVQMSPKMFAPANGGRDLAGFNIYRAIEGGEYTLLDFTTEMSYVD
                     TDEDLVIGTMYCYMVTAVWQGETGDVCESAFSEVACELWTSIGDNDGATASFSLYPNP
                     ASEYVTITTSGDLKRVTVYNALGQLVVDEIITGKQYELAVSSYTIGVYMVRVETEAGI
                     TTRTLTIQR"
BASE COUNT          545 a          491 c          503 g          581 t
ORIGIN      
        1 tagatcttaa aggtgtcctt tctgctgcgt cgatccggga ggaagcagcg gctcccaata
       61 ccagcgcata ttatcataat cggtcgttgc accgtcgtag ttggggggtg tttctgagtc
      121 ggcatagccg ataagagttc tggtccctga accggctgtc ttatatgtcc tgaaataagg
      181 gctggtataa tagccattat ctccccacaa gatatcaacg atcaggttgg aggtacctgt
      241 ccattcaaaa tcggcaatat ctaaaatctg ccatcctgta gcggtagcag gagtatagga
      301 agatgcagag aaaacctgcg ttgcgccggt catatctgca tatgcacctg ccgtcaatga
      361 tgtagcggtg gtctccttaa tcaggattat aacattattc agatatttgt tcccagtggg
      421 gatttgctga aaattccagg caagttttgt aaacttcttt ccagtcggag agccaagttc
      481 tgaggccagg tagagttgtt gtgtatgaca attttcccag taattgcccc aggggccata
      541 ataagccgga taggagccat aacctgtacc ggtacctatt tcaacgaaat tatccggagg
      601 gacaacattg ataaattcca catcatcaaa ataggctttg ggtatatcgg taccttcagc
      661 acctgcaaag aaatccactc ccccaagctg gttaatacca gtggtggaat aagatgtata
      721 gtgaaatggc caggtataaa ttgccgttcc attaatatat aaggtaatga gatcggcatt
      781 caggtctata tggtttacaa tcggaaacca tgtatttttt ggataagtaa atgtgattgg
      841 tgttgcactt ccggcataaa gatttcccgt gccgtcagta tgaaaataga tttcaaatgc
      901 ccattgaata ccgggcgttt cattgtgctg gatattataa tagccagaat atccagtctg
      961 aacataataa tcgaaattta cctcccagac gcctgaggtt ttattcccca gtttaagaac
     1021 caggtcagta gcgcccggat tcaaatcgac caggactgat tttgtcgggc tactggcaaa
     1081 atcggtagaa atctgcccgt cttccgcaga tccgggaagg ttcgaccagg tagtccacca
     1141 ggtcggattc tgggctgcaa tgtaacctcc gacagtatac gactcgaaat tatccgtata
     1201 ctgtgcaaaa ggttttagcg gaagcatcaa aagcaataat gcaaccgctg ttaaaaattt
     1261 agtaaaattt ttcatggcat ttttattagg ttaatattag agttggcact ccatacatat
     1321 taattccatc aagacgtgtt aaagataaga caattatttt atgtaatcag ttgcatgcca
     1381 caaaaaaaaa aggccggcga ggccggcctt tcatttatca gttatcctgt gatattatct
     1441 ctggatggtc agtgtacggg tggtaatgcc agcttcggtt tcaacgcgta ccatatacac
     1501 gccgatcgta tagctcgaca cagccagttc atactgtttg cccgtgatga tctcatcaac
     1561 caccagctgt cccagtgcgt tgtatactgt cacccgtttc aggtcgcctg aagtggtgat
     1621 ggtaacatat tcgctggccg ggttcgggta gaggctgaag cttgctgtag cgccgtcatt
     1681 gtcgccaatg ctggtccaca gttcgcaggc aacttccgag aatgcgcttt cgcaaacatc
     1741 tccggtttcg ccctgccata cggcggtcac catgtagcag tacattgtac cgattaccag
     1801 atcctcatcc gtatcaacat aggacatttc agtggtgaaa tcaagcagtg tgtattcacc
     1861 gccttcgata gcacggtaga tattgaatcc agccagatcc ctgccgccgt tagccggggc
     1921 aaacatcttc gggctcatct gtaccgggct cgtggccagg ctctgtccgt tgatggcact
     1981 cggggttacc gtcttgctca tcggcatcac tgcgccggtt accgtctgca ggtggcccgt
     2041 aatgttccag ttgtagtcca gttccgggtt gatctgcagc agggtctgcc agccgccgaa
     2101 gttcatcatg ttgccgtagc
//
LOCUS       DAOQXS010000474         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_61563_length_2087_cov_3.047244, whole genome shotgun sequence.
ACCESSION   DAOQXS010000474 DAOQXS010000000
VERSION     DAOQXS010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61563_length_2087_cov_3.047244"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKM34_11270"
     CDS             <1..321
                     /locus_tag="PKM34_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84217.1"
                     /translation="AMNLDSIPFGDLRFATDAWLSAPGYKFKNKILLVISVETEKGTI
                     LWQSIPADLQLIDEKGWNHIYNQALYYHSTPGCTLKIYFWNQSDIPLLLDDFRVMVFL
                     PATS"
     gene            394..>2087
                     /locus_tag="PKM34_11275"
     CDS             394..>2087
                     /locus_tag="PKM34_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84218.1"
                     /translation="MKRIALIVILWVVFLLFAFKLSAQYIEVSQPEINGPVFIGYFDG
                     ERMQKRVVSDYKTNPVARLDIKGKIVNLDLTVFRDGYENRVSNPSREAIQWLEPGAPV
                     EGDLHSDIAYTRDGTMFAVIYQNSDNMTFFDAQTCEPIETVQLVRQPVDLYMGEQNAY
                     VACHEGRSIVAVSLEDFSIVNYIPVDGYPCQVEVSPGEDTAFIACDSYRDGWMIAVDM
                     ANNNEVLYLTHDPYFHHYGWGGAMGRTLYKFSKFHLSPKGGQFICGDTSTYQPTIFNT
                     SNGHPVKTFWIGGWRGAGYSPTGDTIYIYSNNEDTVKMLRVNADDFTVIDAIYSDIGC
                     MIGFVSYTDLAISHDGNEVLVSDRANDRYCRFDFDDYTCQVITPWILNEDAHIFSSSD
                     GQYAVVANYLKLQIVNLETGALNDNWPSSMRVGSPLCVSPFENTLVVGNSPGMGIEKL
                     WVVDFSNVNTLIQEASITCGEPPEADMPHVAAVSENGQKAFSVNYLTRDISIIDIYNN
                     TLDTLIYCEDISGLKLIPRTDYALIYGEQKVFSHIVSQLDFSEVAEIQAGGIRDALV"
BASE COUNT          515 a          494 c          513 g          565 t
ORIGIN      
        1 gcgatgaatc tcgacagcat tccctttggt gatttgagat ttgcaacgga tgcctggctc
       61 agcgctccgg gttataaatt taaaaataag atcttactgg tgatttcggt tgaaacggag
      121 aaaggaacca tcctctggca aagcatcccg gccgatcttc agctaattga tgaaaaggga
      181 tggaaccata tatataacca ggctttatat tatcacagca ctcccggctg caccctgaaa
      241 atctatttct ggaatcaaag cgacatcccg ctgctcctgg atgatttccg ggtgatggtg
      301 tttcttccgg cgacctcctg ataagttatt ctttacttat ttgtcattga cattaataat
      361 atttaaattt gttgcgcttt aaaaatcctg gttatgaaac gcatcgcatt aattgtcatt
      421 ctctgggtag tctttcttct tttcgccttt aaactttccg ctcagtatat cgaagtttct
      481 caaccggaga taaacgggcc ggtttttatc ggttactttg acggtgaacg catgcaaaag
      541 cgggttgtca gcgattacaa aacgaacccg gtggcacggc tcgatattaa gggtaaaatt
      601 gtcaatttag acttgaccgt cttcagggac ggttatgaga acagggtttc caacccttcc
      661 cgcgaggcca tacaatggct cgagcccggt gcgccggtcg aaggcgacct tcactccgat
      721 atcgcctata cccgcgacgg aacaatgttt gccgtaattt accagaacag cgataacatg
      781 actttttttg atgcccagac ctgcgagccg atcgaaacgg tgcagttggt ccggcagccg
      841 gtcgacctgt atatgggtga acaaaatgct tatgtggctt gccatgaagg ccgcagtatc
      901 gttgctgtct ccctggagga tttcagcatt gtcaattata ttcccgtcga cggctatccc
      961 tgccaggttg aggtgagccc gggggaagac acggccttta ttgcgtgcga cagttaccgc
     1021 gacggctgga tgatcgccgt cgatatggcc aataacaacg aggttttgta ccttacccac
     1081 gatccctatt ttcaccatta tggctggggc ggcgccatgg gccggaccct ctataaattc
     1141 agtaaatttc acctttcacc caaaggcgga cagtttatct gcggcgatac gtccacatac
     1201 cagcccacca tttttaatac atcaaacggc catccggtaa agacattctg gatcggcggc
     1261 tggcgcgggg cgggctattc ccccaccggc gatacgattt acatttattc caataatgaa
     1321 gacacggtta agatgctgag ggtcaatgca gatgatttta cggtaattga cgctatttac
     1381 agcgatatcg gctgcatgat cggctttgtc agttacaccg acctggcgat cagccacgat
     1441 ggcaacgaag tgctggtcag cgacagggca aacgaccggt actgccggtt tgactttgac
     1501 gattacacct gccaggttat cactccctgg atcctgaatg aggatgcgca tatattttca
     1561 tcttcggatg ggcaatatgc cgttgtggct aattatctga aattgcagat agttaacctg
     1621 gaaacggggg cgttgaacga caattggccg tcaagtatga gagtgggttc gcccctttgc
     1681 gtatcgccat tcgaaaatac gctggtggtc ggtaattcgc ccggcatggg gattgaaaaa
     1741 ctttgggttg ttgatttcag caatgtgaat actttgatcc aggaagcttc cattacctgc
     1801 ggtgagcctc cggaagccga tatgccgcat gtcgcggcag tctccgaaaa cggacagaaa
     1861 gctttctccg tcaattacct gacacgggat atcagtatca tcgatattta taacaatact
     1921 ttggatacat tgatctattg tgaggatatc tcgggcctta agcttattcc gagaaccgat
     1981 tatgccctca tctatggcga acaaaaggtg ttcagtcata ttgtctccca gctcgatttc
     2041 tcggaagttg ctgaaataca ggcaggagga atcagggatg cgctggt
//
LOCUS       DAOQXS010000475         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_61792_length_2082_cov_3.564874, whole genome shotgun sequence.
ACCESSION   DAOQXS010000475 DAOQXS010000000
VERSION     DAOQXS010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61792_length_2082_cov_3.564874"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1051
                     /locus_tag="PKM34_11280"
     CDS             98..1051
                     /locus_tag="PKM34_11280"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNQ84219.1"
                     /translation="MRVVEAIAKSRSPLFTFEILPPVKGSDINSIYRAIDPLMEFEPA
                     FIEVTYHREEILYKEFPDGSIEKRVLRKRPGTVGISAAIKYKYKIEVVPHIICGGFNR
                     EETENGLIDLHFLEIYNLLAIRGDNLPGEKFFKPEKGGNQFAVDLIRQIMDLNKGKYL
                     DNELVNNAPTRFSVGVAGYPEKHAEAPNMRTDIRHLKEKIDAGGEYIVTQMFFENRFY
                     FEFVKQCREAGITVPIIPGLKPVSTAGQLNTLPKTFNISIPEDLQKEVEKCRDSKQVR
                     QVGIEWCTMQSKELMQAGVPALHYFTMGNSDNIREIVKHIF"
     gene            1246..1392
                     /locus_tag="PKM34_11285"
     CDS             1246..1392
                     /locus_tag="PKM34_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84220.1"
                     /translation="MVAAFKKQPDIAIGNVIGSNIFNILSILGIAAMVEPIEAPGSIL
                     PIPW"
     gene            1637..>2082
                     /locus_tag="PKM34_11290"
     CDS             1637..>2082
                     /locus_tag="PKM34_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84221.1"
                     /translation="MKKIYLLFMICLMGVAVFAQPVGRQKVIVEIGTGTWCTYCPGAA
                     MGADDLVLYGCQVGNIEYHNGDPYANTASDYRNSYYNVSGYPTAHFDGVLEYVGGSHT
                     ESMYPNYLPLYQQRMAIPSDFLIDIYGEHTGNTYNIQLVISQTNGT"
BASE COUNT          596 a          466 c          489 g          531 t
ORIGIN      
        1 acggcttcag tcgtcggact acctaaaaaa agtgaatata tggacttttg agacagcctc
       61 ttgaatctgt gaaaatctgt gagaaaaatc aatacctatg cgagtagttg aagcaattgc
      121 aaagtcaagg agccccctgt tcacctttga aatccttccg ccggtgaagg gaagcgatat
      181 caattcgatc tatcgtgcca ttgacccgtt gatggagttt gagcccgctt ttatcgaggt
      241 tacctatcac cgggaagaaa tattatacaa ggaatttccg gatggaagta ttgaaaaaag
      301 agtcctgaga aaacgtcccg gaacagtggg tatttcagca gcaataaagt acaaatacaa
      361 gatcgaagtc gttccgcaca ttatttgcgg cggatttaac agggaggaga ccgaaaacgg
      421 ccttatcgac cttcatttcc tggaaattta taacctgctg gcgatcaggg gagataatct
      481 tccgggcgaa aagttcttca aaccggaaaa agggggcaat caatttgctg tggaccttat
      541 ccgccagatc atggacctga acaagggcaa atatcttgac aacgagctgg tgaataacgc
      601 cccgacccgg ttttcggtcg gtgtggccgg atacccggaa aagcatgccg aagcgccaaa
      661 catgagaacg gatatcaggc atttgaaaga aaagatcgat gccggcggtg aatacatcgt
      721 cacgcagatg ttttttgaaa accggttcta tttcgaattt gtaaaacaat gccgggaagc
      781 gggcataacg gtacccatca ttcccggcct gaaaccggtt tcgaccgccg ggcagctgaa
      841 tacgcttcca aagactttca atatctccat tccggaagat ttgcagaaag aggtggaaaa
      901 atgcagggac agcaaacagg tccggcaggt ggggatcgaa tggtgcacca tgcagtcgaa
      961 agagctgatg caggcgggtg tgccggcgct gcactatttc accatgggca actcggataa
     1021 tatccgggag atcgtgaaac acatatttta gttcaaagtt catccgcaag ctggcggatc
     1081 aaagttcaaa gtttaattaa aattatgaat taagaattca atattaaccc agatattgac
     1141 ttatattcta ttcataatca tttctttagc tgtattgtat ctgggggtca gcgggcttgt
     1201 caaaggggcg ccgtcggtac cagcactccc gaactggcca cctccatcgt cgcagcattt
     1261 aagaagcaac ccgatattgc cattggcaac gtgatcggct ccaatatctt caatattctg
     1321 agcattctgg ggattgcggc gatggttgag cccatcgaag cgcccggttc aatcttaccg
     1381 ataccctggt aatgatcggc atgtcgttgc ttctgctgcc ttttattaag acaggcttca
     1441 ctttacgccg ttgggaagcg gctttgatgc tggcagttta catcggttac atcgtttacc
     1501 tgcttcaggg gaattgaatc cctcctggtt ttacccacac taatttagac agattataaa
     1561 tatctggcat tttatatata tgcctgaaaa taatttattt ttagacccta aaattctgaa
     1621 tcaaaaaaca ttaccgatga aaaaaatcta cttacttttc atgatctgcc tgatgggtgt
     1681 tgctgttttt gcacaacctg tcggaaggca aaaagttatt gttgaaattg gaaccggaac
     1741 ctggtgcacc tactgcccgg gcgcagccat gggagccgat gatctcgttt tatacgggtg
     1801 ccaggttgga aacattgagt accataacgg cgatccgtat gccaatacag cttccgatta
     1861 caggaacagc tattacaacg tatcaggata tccgacagcc cacttcgacg gggttctcga
     1921 atatgtcgga ggaagccata ccgaaagcat gtatcctaac tacctgccgc tgtaccagca
     1981 acggatggcc attccaagtg atttcctcat tgatatctat ggtgaacata cagggaatac
     2041 atacaatata caactcgtca tttcgcaaac aaacggcacc tg
//
LOCUS       DAOQXS010000476         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_65614_length_2007_cov_3.041496, whole genome shotgun sequence.
ACCESSION   DAOQXS010000476 DAOQXS010000000
VERSION     DAOQXS010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65614_length_2007_cov_3.041496"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..478
                     /locus_tag="PKM34_11295"
     CDS             101..478
                     /locus_tag="PKM34_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84222.1"
                     /translation="MDTRKFEYLETIRETIETGDYEELDDYIEDIRDQVKADPEFQEL
                     FGEIKSRNYGDAISLIDEFIYQDIQSGQDDFFNEESLNRLGGLDYPKNLEGFTEEGVP
                     EEISFEEFIEDDFVGGAGQEEEY"
     gene            500..>2007
                     /locus_tag="PKM34_11300"
     CDS             500..>2007
                     /locus_tag="PKM34_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84223.1"
                     /translation="MKKFFWISALFLLSAGIALLILARYAEHVAEPYILTFLEHNKPG
                     HHKIEYGKIRLNLLNSTVNIRDVRVFPEPEYSGNENVWMVLNVGLIKLKRIDLWELFF
                     HKNLYISDFIILRPETRLHLPDRITSQSVENVTAEEDVQVKPQLLKSIALDKMIISGG
                     TFRLYRNEDIIASSDNIGFLARNISLVKNTDDDPLGYSYGEVKFILTDLWFQSETGLY
                     DMGLDKFTVVKKDSSVILEGFRMKPKYGREEFSKQLKSQDDRFDIDIGKIEVAGIGYR
                     RFLEGNPLEITRIMIDGMTADIYRDKNVPFDLMNYPEYYNELFLKLSIPLQINAVEID
                     NSRLIYSELATGAAQAGEIIFEDFKLVSYKLNNRPVNDSVEDFIQINVKAKIMGEGPL
                     NADLVLPLEGNTRAIRCSGSVGAMKVVPLNGMLEPSINIKFNDGLLTRMTFAFEGDER
                     LSNGWMEFLYKDLNVAILKKEPGKEWGFLSMLANTVTLSNNPHSGKDPKIVA"
BASE COUNT          587 a          441 c          461 g          518 t
ORIGIN      
        1 acaaagatca tcatgttttt ttcaatcaac ccgctctttt tagcaaaaat atgcttacat
       61 ttaaactgca tttgataatg gataactaat ttttgctgca atggatacca gaaaattcga
      121 gtacctggaa acaatccgtg aaaccattga aacgggtgat tatgaagaac tggatgatta
      181 tattgaagat atccgcgacc aggtaaaagc cgatcccgaa ttccaggaac tcttcgggga
      241 gatcaaaagc cgtaattacg gcgatgccat ttctttgatc gatgagttta tctaccagga
      301 tatccagtcg ggccaggacg atttttttaa tgaagaatcg ctgaacaggc ttggcggact
      361 ggattatcca aagaatttgg agggctttac cgaagaagga gtgccggaag aaatttcgtt
      421 tgaagagttt attgaagatg attttgtcgg aggtgcaggc caggaggaag agtattaata
      481 tctggccgtt cgacgcacga tgaagaaatt cttctggatt tcagctttat tcctcctttc
      541 tgccgggatt gctctcctga tcctggcccg ctatgcggaa catgtcgccg aaccgtatat
      601 ccttaccttc cttgaacaca acaaaccggg ccaccacaaa atcgaatacg gtaagatcag
      661 gcttaacctg ctcaacagta cggtaaatat ccgtgatgtc cgggttttcc cggaaccgga
      721 atattcaggg aatgaaaacg tctggatggt attgaatgtc ggccttatca aactgaaacg
      781 gattgacctt tgggaattat ttttccataa gaacctctat atcagcgatt ttattatact
      841 ccggcccgaa acccgtcttc atctgcctga ccggatcacc agccagtctg tcgaaaatgt
      901 aactgccgaa gaagatgtgc aggtaaaacc gcagctgctc aaaagtatag cgctcgataa
      961 aatgatcatc tccggcggta ctttccgcct ttaccgcaac gaagatatta tcgccagttc
     1021 cgacaatatt ggctttttag cacggaatat cagcctggtt aaaaatactg atgatgaccc
     1081 gctgggctat tcttacggag aagtgaagtt catactgacc gatctgtggt tccagtctga
     1141 aaccggcctc tatgacatgg gcctggataa attcaccgtc gttaaaaaag attcatccgt
     1201 tatccttgaa ggattccgga tgaaacccaa atacggcagg gaggaattct caaaacaact
     1261 gaaatcacag gatgaccggt tcgatattga catcgggaag atagaggtgg ccgggatcgg
     1321 ttaccgcaga ttcctcgaag ggaacccgct ggaaattacc cggatcatga tagacggaat
     1381 gaccgcagat atttatcgcg ataaaaatgt tccgttcgat ttgatgaact acccggaata
     1441 ttacaacgag ctattcctga aattaagcat ccctcttcag atcaatgctg ttgagatcga
     1501 caattcccgg ctgatttaca gcgaactggc aaccggtgcc gcccaggctg gcgaaattat
     1561 tttcgaagac tttaaactgg tttcgtacaa actgaataac aggcctgtca acgactctgt
     1621 cgaggatttc attcaaataa acgtaaaggc taaaatcatg ggagaaggtc cgctgaatgc
     1681 cgacctggtt ttgccgctgg aagggaacac aagggcaatc aggtgctctg gcagcgtggg
     1741 ggctatgaag gtcgtgccac tgaacggtat gctggaacct tccatcaata taaaattcaa
     1801 cgatggcctg cttacccgga tgactttcgc ttttgaagga gatgaaaggc tttcaaacgg
     1861 ctggatggaa tttctctata aagatcttaa tgtggccatt ttgaaaaaag aacccggcaa
     1921 agagtgggga ttcctctcca tgcttgcaaa tacggtcaca ctctccaaca atccccattc
     1981 cggaaaagat ccaaaaatag tagctat
//
LOCUS       DAOQXS010000477         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_65713_length_2005_cov_3.284615, whole genome shotgun sequence.
ACCESSION   DAOQXS010000477 DAOQXS010000000
VERSION     DAOQXS010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65713_length_2005_cov_3.284615"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PKM34_11305"
     CDS             complement(<1..1485)
                     /locus_tag="PKM34_11305"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNQ84224.1"
                     /translation="MDKIRILWVDDEIEILKPHILFLEQKGYEVETSNNGDDGLEMIL
                     NRYYDIVFLDENMPGLTGIETLERIKTRYPNLPVVMITKSEEESIMEDAIGSNIADFL
                     IKPVKPNQILLSLKKNLENKKLFSEKTTSIYQQEFRNISMDISNNPDYSGWMDIYRNL
                     TRWELELEKSNDASMQEVLQHQKSEANQVFSKYVENNYLNWINGRSRTYPVMSPNLFK
                     DKVFPLLDSNESLFVILIDNLRYDQWKAIQPILEEYFRIDREEMYYSILPTTTQYARN
                     ALFAGLMPLEIERKYPKYWVAEEDEGTKNQFEAELLGEYLKRYGRDIKYSYNKILNLT
                     AGRKLVETLPNLMSNKLSVIVYNFVDMLSHARTEMEIIRELADDEKAYRSLMVSWFNN
                     SPLLDIIKYLAEKKVNVVITTDHGSVKAQDPIKVIGDRNTTTNLRYKSGRSLNYSPKE
                     VFEVRNPHDAYLPRTNVSSSFIFCRRNDFFAYPNNYNYYVNYYRN"
     gene            1660..>2005
                     /locus_tag="PKM34_11310"
     CDS             1660..>2005
                     /locus_tag="PKM34_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNQ84225.1"
                     /translation="MEFSASQIAAVLGGQTEGNPDVKISRLSKIEEGEPGTLSFLANP
                     KYTPYIYTTKASIIIVSRDFIPDQPVSATLVRVDDAYTAFAKLLELYNQIKNEKKGIS
                     SLAFISKSAVIGP"
BASE COUNT          532 a          455 c          408 g          610 t
ORIGIN      
        1 gttcctgtaa taattcacat agtaattgta attgttggga taggcaaaga aatcatttct
       61 ccggcagaat atgaaagatg aactgacatt ggtcctggga agatatgcat catgcggatt
      121 acgcacctcg aatacttctt tcgggctgta gttcaggctt ctgcctgatt tataccgaag
      181 atttgttgtc gtattccggt ctccgatcac cttaatcgga tcctgcgctt tgacagaacc
      241 atggtcagtc gtaataacca cgtttacctt cttttctgcc aggtatttga taatatcgag
      301 caacggtgaa ttattaaacc acgaaaccat cagcgaccgg taggcttttt catcatccgc
      361 caattccctg atgatctcca tttcagtccg ggcatgggag agcatatcta caaagttgta
      421 aacgatcaca gatagcttgt tcgacatcag gttaggaaga gtttccacca gcttccggcc
      481 ggcggtcagg ttaaggattt tattgtagga atatttgata tcacgcccgt accttttcag
      541 gtattcaccc agtaattccg cctcaaactg gtttttggtc ccttcatcct cttccgcaac
      601 ccagtatttg gggtacttgc gttcgatctc caggggcatt aggcctgcaa acaatgcatt
      661 ccgggcatat tgcgttgtcg tcggaagaat gctgtagtac atctcctccc tgtcgatcct
      721 gaaatattcc tcaagtatcg gctgtatggc cttccactga tcgtaccgca ggttatcaat
      781 aaggataacg aacagtgact cattgctgtc aagcaatgga aagaccttgt ctttaaacaa
      841 attaggcgac ataaccgggt aggtccgcga acgaccgtta atccagttaa ggtaattgtt
      901 ctccacatat ttggaaaaaa cctggtttgc ctcgcttttc tgatgctgaa gaacctcctg
      961 catactggcg tcattcgatt tctccagctc cagttcccac cgggtcaggt tgcggtaaat
     1021 atccatccat ccggaataat ccggattatt ggatatatcc atacttatat tcctgaattc
     1081 ctgctggtaa attgaagtgg ttttttcgct gaatagcttt ttattctcca ggttcttttt
     1141 caggctcagg agtatttggt tgggcttaac cggtttgatc aggaaatccg ctatattcga
     1201 accgatggca tcctccatga tcgattcctc ttcgctcttg gtgatcataa ctaccggcag
     1261 gttcggataa cgggttttga tcctttccag agtctcgatc ccggtcagtc cgggcatatt
     1321 ctcatccaga aatacgatat cataataacg gttaaggatc atttccaggc catcatcccc
     1381 attattggaa gtttccactt catacccttt ttgttcaaga aaaagaatgt gtggtttcaa
     1441 tatttcaatc tcatcatcaa cccagagaat tcttatctta tccatgctta aatattattt
     1501 agaataaaga cttatattaa gaaatgaaaa taatagatat ttattgttca gatgatgaat
     1561 tatccgtgaa aagtggtttc gacaaacagg ccttcaaatt tataatttta atacctttgt
     1621 cccacctaaa aacattgttt tcaaataaat ccgatccgca tggagttttc agccagccag
     1681 atagccgctg ttttgggagg acagacagaa ggtaatcctg acgtaaagat cagcaggcta
     1741 tccaagattg aagaaggaga accgggaacg ttatcatttc ttgccaatcc aaagtacacc
     1801 ccgtacattt atactacgaa agcatccatt attattgtga gccgggattt tattcccgac
     1861 cagccggtat cagccaccct ggtccgtgtt gatgacgcct ataccgcatt tgccaaactt
     1921 ttggagctgt ataaccagat caagaatgag aagaaaggga tttcctccct ggcttttatt
     1981 tccaagtcgg ctgttatcgg tccgg
//
LOCUS       DAOQXS010000478         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_66768_length_1986_cov_2.716209, whole genome shotgun sequence.
ACCESSION   DAOQXS010000478 DAOQXS010000000
VERSION     DAOQXS010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66768_length_1986_cov_2.716209"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKM34_11315"
     CDS             <1..295
                     /locus_tag="PKM34_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ84226.1"
                     /translation="ISFDFKKIIAHTVAKRLANECGIGIRSGCHCAHLIVKRILGVAP
                     PFEGIQRLIVLAAPGLNLPGVARVSLGIENTGEDAERLVKSLETIVGKKRRLN"
     gene            complement(298..1176)
                     /locus_tag="PKM34_11320"
     CDS             complement(298..1176)
                     /locus_tag="PKM34_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84227.1"
                     /translation="MKKFHTLGKAVISVVCICLMHNFSVAQVADVYNGTVLPQVKETI
                     QAPVMEPVRSLELYNNGPLITHYGIGYNGADISELQNPSLGMTVSGFGHQFSLGYKIA
                     DDFTVTDPGGWNIVGIAFYAYQAGSPSTSNFTAVYFAIYDGDPMLPGSNIIYGDFVTN
                     RMTSTGFTGIYRVLETAPTDNNRPIMVNHCEFDLTLGPGTYWLVWMSDGADIYGYEWA
                     PPVTILGQAPTGNGLQFTTAWGGAYDSGTMTQQGFPFVIFGRAGQAAIPVAGWALGIG
                     LVLIIGLAVLRYRRIL"
     gene            complement(1267..>1986)
                     /locus_tag="PKM34_11325"
     CDS             complement(1267..>1986)
                     /locus_tag="PKM34_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNQ84228.1"
                     /translation="SWFWIIAWGVISLFTILMTLFYSNIIVPLFNKQTPLEPGELRDA
                     IEEFAGKTGFSLKNIYVIDGSKRSTKANAYFTGLGRKKRIVLYDTLIKDHPVDELVAV
                     LAHEIGHYRKKHTLIGTLISVLQTGLILFILSRFLDNPHLSGALGASQPSFHLGLVAF
                     GILFTPISLITGLGLNILSRMHEFQADRFAAVNHNAESLRNALVRLSVNNLSNLRPHD
                     LYVFFHYSHPPLLKRLKRLSE"
BASE COUNT          538 a          472 c          511 g          465 t
ORIGIN      
        1 catatcattt gatttcaaga aaattatcgc acatacggtt gccaaacgcc tggccaatga
       61 gtgcggcata gggatccgtt cgggctgcca ttgtgcccat ctgatcgtca agcggatact
      121 tggcgtcgct ccgcccttcg aaggcattca gcggctgatt gtactcgctg cacccgggtt
      181 gaaccttccg ggcgtagcac gggtcagcct tgggattgaa aatacgggcg aagacgctga
      241 acgactggtg aaatcactgg aaacaatagt cgggaaaaag aggcgcctga actaatgtta
      301 aaggatcctt ctgtatctga gaacggccag cccgatgatc aggaccagac cgatgcccag
      361 agcccagccg gcgacgggaa tagccgcctg gccggcacgg ccaaagataa caaaaggaaa
      421 tccttgctgg gtcatagttc cgctatcata agctccaccc caggccgttg taaattgtag
      481 cccgttgccg gtaggggcct gtcccaggat ggtaaccgga ggggcccact catatccata
      541 gatatctgct ccatccgaca tccagaccag ccagtaggtg ccggggccca atgtcaggtc
      601 aaattcgcag tgattgacca taatgggccg gttattgtca gtaggcgctg tctcaaggac
      661 cctgtatatt ccggtaaagc cggtagaggt catcctgttg gttacaaaat caccataaat
      721 gatatttgag cccggcaaca tcgggtctcc atcgtatatg gcaaaataga ccgcggtgaa
      781 gtttgatgtg gagggtgaac ccgcctgata ggcataaaat gcgataccca cgatattcca
      841 tccacccgga tcggtaacgg taaaatcatc ggcaatttta taacccagtg aaaactgatg
      901 accaaacccg gaaacggtca tgcccaggga aggattttgt aattcactga tatcggcccc
      961 attataaccg ataccataat gagtgatcag cggcccgtta ttgtataatt ccaggcttct
     1021 gaccggctcc attactggtg cctggatagt ttcttttacc tgcggcaata ccgtaccatt
     1081 ataaacatcg gcaacctgtg ctacagagaa gttatgcatc agacaaatgc aaacgacgga
     1141 aataaccgcc tttccgaggg tatggaattt cttcataatt aattggtttt taaattttga
     1201 tgcaatataa tgattcggga tcagaaaagc aataaatttc tataaaacct cacgttcatt
     1261 tttcggtcat tcacttaacc ttttcaatcg tttgaggagt ggtggatggg aataatggaa
     1321 gaaaacgtaa agatcgtgcg gacggagatt acttaaatta ttgacagata aacggacgag
     1381 cgcattccgc aggctttccg cgttgtggtt cacggctgcg aagcgatcag cctggaattc
     1441 atgcatgcgc gacaggatat tcaatcccaa ccccgtaata agcgatatgg gagtgaacag
     1501 aatgccgaag gcgaccagtc ccaggtggaa gctgggctgg ctggcaccaa gggcgcccga
     1561 aaggtgcgga ttatcgagga agcggctgag gataaaaagg atcaggccgg tttgcaaaac
     1621 agaaataagc gttccgatca gggtatgctt tttccggtaa tggccgatct catgagccag
     1681 gacagccacc agttcgtcga ccggatgatc tttgatcagg gtatcgtaaa gaacgatccg
     1741 ctttttgcgc cccaatcccg taaaataggc attggctttg gttgatcgct tcgagccgtc
     1801 gatcacgtag atatttttca ggctgaaccc ggttttaccg gcaaattcct cgatagcgtc
     1861 gcgcagttca cccggctcaa gcggtgtttg tttgttgaac aggggcacaa tgatgttgga
     1921 ataaaataaa gtcatcagaa tcgtaaagag gctgatgacg ccccaggcga tgatccagaa
     1981 ccaact
//
LOCUS       DAOQXS010000479         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_67631_length_1970_cov_3.797389, whole genome shotgun sequence.
ACCESSION   DAOQXS010000479 DAOQXS010000000
VERSION     DAOQXS010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67631_length_1970_cov_3.797389"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..893
                     /locus_tag="PKM34_11330"
     CDS             237..893
                     /locus_tag="PKM34_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84229.1"
                     /translation="MKRIAFLIAGLVAFTMVNAQTETNENAGEINTVFGKNGRPAVGW
                     FIGLDHGYTQFESRDVWTGGLNFGMIIDHKFSIGLTGTGWTNRNGMYYENISGDDGAY
                     LEGGYGGLLLEYTLFPKSLVHLTIPVTIGGGGVSYISESDIPVWDEDEWDFDNVVIDD
                     DLFFVVEPGIRAEMNVFRFMRLNAGVSYRHTEGLQMINTSEDLMNNFTATVGLKFGKF
                     "
     gene            993..>1970
                     /locus_tag="PKM34_11335"
     CDS             993..>1970
                     /locus_tag="PKM34_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNQ84230.1"
                     /translation="MSYWFLMNAQFKNLFPVYTWLPGYRISFLKWDLIAGTTLAFFAI
                     PTSMAYATLAGLPPQTGIYCYLFAGLLYFVFGTSRHLAVGPTSAISIIVATSIGALAG
                     NDPAKAITLASATALLMAGLFLGAYIIRLSSLINFISDTVLTGFKAGAALVIASTQLP
                     KLFGIPDAGDNFIDRIRNLITNLGETNITVLVFGMIALILLLLGNHYLKGKPVSLIIV
                     IISILLVSFAGFKEFGITVVGEIPKGIPALRWSVPNSHEFNDIFFLALACFFLSYIES
                     ISAARSIAREKGYEIDPQQELLALGAANLASSLGGGYAVAGGLSQSTVNA"
BASE COUNT          474 a          448 c          490 g          558 t
ORIGIN      
        1 ttagttcaaa gttcaaagtt caaagttcaa agttcaaagt tcaaagttca aagtatatta
       61 ttcccttttc atttttcact attccctatt cactattcct tttctaaacc tcaccggctg
      121 gattccgcag ttggcgggta ttttgtttcg caggtagaga gcggcgtgta ctttcgcatc
      181 atcaaattta attaatcaca atcaacatta ttaatcacaa ttaaaaacta agtacaatga
      241 aaagaattgc ttttttaatc gcgggtctgg ttgctttcac aatggtaaat gcgcagacgg
      301 aaactaatga aaacgccggt gagatcaata cggtgttcgg taagaacggc aggccggcag
      361 tcggctggtt tatcggcctg gatcatgggt atacccaatt cgaaagccgg gatgtatgga
      421 caggtggatt gaattttggt atgataatcg accacaaatt ttcgatcggc ctgaccggta
      481 ccggatggac caaccgtaac ggaatgtatt acgagaatat ttcgggcgac gacggggctt
      541 accttgaagg aggttacggc ggattgttac tggaatacac cctgtttccc aaatcgctgg
      601 tgcacctgac catcccggtc acgatcggtg gcggcggtgt ttcctatatt tcagaatccg
      661 acatcccggt atgggatgaa gatgagtggg atttcgacaa tgtggtgatc gacgacgacc
      721 tgtttttcgt ggttgaaccg ggaatccgcg ccgagatgaa cgttttccgc ttcatgcggc
      781 tgaatgcagg ggtaagctac aggcataccg aagggttgca gatgatcaat acgtcagagg
      841 acctgatgaa caacttcacc gcaacggtgg ggctgaagtt cgggaaattc tgacggattg
      901 tgattaatga tgaaagggcg gtgccgaaag ggccgccctt tttgcgtttt aaggtttgag
      961 aggatttcgg agaaagtgtt taaatttgac atatgagtta ttggttcctg atgaatgcgc
     1021 aattcaaaaa tcttttccct gtatatacct ggctgccagg gtaccggata tcctttctga
     1081 aatgggatct cattgccggg acgacactgg cattcttcgc catccctact tctatggctt
     1141 atgcgaccct tgccggtctc ccgccacaga ccgggatcta ttgctacctc ttcgccggct
     1201 tgctctattt tgtcttcggc acctctcgcc acctggctgt cgggcccact tcagccatct
     1261 ccatcatcgt ggcaacttct attggcgctt tggcagggaa tgacccggca aaggcgatca
     1321 cgcttgcctc tgcgacagca ctcctgatgg caggactgtt cctgggtgct tatattatca
     1381 ggttgagctc gctgatcaac tttatcagcg atacggtact gacaggattt aaggcaggtg
     1441 ccgccctcgt tatcgcttca acccaattgc caaaattatt cggaataccg gatgccggcg
     1501 ataattttat cgacaggatc cggaacctca taacaaatct gggggaaaca aatatcacgg
     1561 ttctggtttt cggcatgatc gccttaatcc ttctccttct gggtaatcac tatctgaaag
     1621 gcaaacccgt ttcgttgatc attgtgatta tatccattct attggtttct tttgctggat
     1681 ttaaggaatt tgggattact gttgttggtg aaataccgaa aggcatccca gcattgagat
     1741 ggtcagtgcc aaatagccat gaatttaacg acattttttt tctggcgctg gcctgttttt
     1801 tcctgtcata tatagagagc atatccgccg cccggtctat tgcaagggag aaaggctatg
     1861 agatcgatcc gcagcaggaa ttgctggccc tgggagccgc taaccttgca tcatcattgg
     1921 gaggtgggta tgctgtggcc ggcggacttt cgcaatccac ggtcaatgcc
//
LOCUS       DAOQXS010000480         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_70122_length_1928_cov_3.209290, whole genome shotgun sequence.
ACCESSION   DAOQXS010000480 DAOQXS010000000
VERSION     DAOQXS010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70122_length_1928_cov_3.209290"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKM34_11340"
     CDS             <1..430
                     /locus_tag="PKM34_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNQ84231.1"
                     /translation="NLNKVNYKLARCCNPQPGDPVFGFVTISRGITLHTQSCPNARQL
                     LTRFGYRKIDVQWKTSPDKNLVRAVINVTGTDRIGIMNEITQIISNDLKTNMISVKID
                     VRKNIFNGIIKLMVKNTSSLNDLIGRISKVSGVSKVVKID"
     gene            513..977
                     /locus_tag="PKM34_11345"
     CDS             513..977
                     /locus_tag="PKM34_11345"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HNQ84232.1"
                     /translation="MRLFTFLIIALSIALVSCDKDNDGNNNGMGGTMTLKHDGSNWSA
                     SLAVAAANTNGVLSVTGSDNSANQCNVTLYNIPGPGTYQLGGSMSNPNTGRWTQGLGQ
                     NDTYSTMVGQGTGTCTITELTASKVSGTFEFTAKNTAGNMVTISEGSFSADL"
     gene            1162..1551
                     /locus_tag="PKM34_11350"
     CDS             1162..1551
                     /locus_tag="PKM34_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNQ84233.1"
                     /translation="MKNTPKKFKPTETELEILSILWNKGPATVRDVNDEMSRSKPTGY
                     TTTLKLMQIMAEKGLVEREMDGRSHIYHALARQEDAQVSMLDRLLETAFGGSASKLVM
                     QTLGNHKTTESELKEIKLLIKKLEEEK"
     gene            1548..>1928
                     /locus_tag="PKM34_11355"
     CDS             1548..>1928
                     /locus_tag="PKM34_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84234.1"
                     /translation="MMTAIGSILSDQVVTALALTLFHSLWEGVLLALVTTGFVYFFRS
                     GRPLFRYSAYYTLLLIMPVMFAVTFFIVYDNQEVSVPPSVHPVSSTQALVSTSSLTNS
                     AISHPVNLASPHWLKIPVDFIENYA"
BASE COUNT          547 a          429 c          421 g          531 t
ORIGIN      
        1 caatctcaat aaagtcaatt ataagcttgc ccgttgctgc aatcctcagc caggcgaccc
       61 ggtattcggc tttgttacta tcagccgggg tataacgcta catacccaat cttgtccgaa
      121 tgcacgccag ttgctgaccc gctttggata ccggaagatc gacgtccagt ggaaaacctc
      181 cccggataaa aacctcgtcc gggcagtcat aaacgtaacg ggtaccgacc gtatcggtat
      241 tatgaatgag ataacgcaga ttatctccaa tgatctaaag accaatatga tatcagtcaa
      301 gatcgatgtc aggaagaata tctttaatgg tattattaaa ttaatggtaa aaaacacctc
      361 ttctttaaac gaccttatcg ggagaatttc aaaggtctcc ggtgtcagca aggtcgttaa
      421 aatagattaa tcttaaatat ttgttaaata ttttcatctt tttctttaac tccttaggtt
      481 tgcacaaatc taaaatttaa aatatgaaga agatgagatt atttaccttt ttgatcattg
      541 ctctatctat tgcccttgta tcctgtgata aggataatga tggaaataac aacggaatgg
      601 gaggcacgat gactttaaaa catgatggtt cgaactggtc tgccagcctg gctgttgctg
      661 cggctaacac aaatggcgta ttatcggtca ccggtagcga taatagcgca aaccagtgca
      721 acgtgaccct ctacaacatt cccggaccgg gaacctacca gctgggcggc agcatgtcga
      781 atccaaatac gggtcgctgg acccagggac ttggccagaa tgatacctat tcaactatgg
      841 tgggtcaagg tacgggaaca tgcacaatta ccgaactgac cgcatcaaaa gtctccggaa
      901 cctttgagtt tactgcaaaa aatactgccg gcaatatggt cacgatctcc gaaggaagct
      961 tctctgccga tctttagaat ctctgtggat ccgttcctat tcgtccaatt cgtgtcatct
     1021 gtgttccatt aaatgatgga tgatcacgaa agcaataaga acggatttat ttttaacatt
     1081 ttttacgaaa tatttcgtaa ataagattga cttcagaaca gaattgatct atctttgtct
     1141 tacgaaaaga ttcgtacaga aatgaaaaac acaccgaaaa agttcaaacc cacagaaacg
     1201 gaactcgaaa ttctttccat tctttggaat aaaggaccgg caacggtcag ggatgtgaat
     1261 gacgagatga gcaggagcaa gcccaccggg tataccacca ctttgaaact gatgcaaata
     1321 atggcagaga aagggctggt ggagagggaa atggatggca ggtcgcatat ttatcatgcg
     1381 ttagccaggc aggaagatgc ccaggtgagt atgcttgacc ggctcctcga aacagctttc
     1441 ggcggttcag cttctaaatt ggttatgcaa acccttggca atcataaaac caccgaatcg
     1501 gagctgaaag agatcaaact gctgattaaa aagttggagg aggagaaatg atgacagcta
     1561 tcggttccat tctttctgac caggttgtta cagcgttggc attaacgctg tttcactcct
     1621 tatgggaggg agtcctcctg gcgctggtta ctaccggttt tgtttatttc ttcaggtccg
     1681 gcaggcctct gtttcggtat tcggcttatt acaccttgct gcttataatg cctgtcatgt
     1741 ttgctgtcac cttctttatc gtatatgata accaggaggt atctgttccg ccatcggtcc
     1801 atccggtctc aagcacgcag gccctcgttt ccacttcgtc acttaccaat tctgccattt
     1861 cccatccggt taacctggct tcgccacact ggttgaaaat accggttgat tttatcgaga
     1921 attatgcc
//
LOCUS       DAOQXS010000481         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_71597_length_1904_cov_2.453218, whole genome shotgun sequence.
ACCESSION   DAOQXS010000481 DAOQXS010000000
VERSION     DAOQXS010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71597_length_1904_cov_2.453218"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKM34_11360"
     CDS             complement(<1..771)
                     /locus_tag="PKM34_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84235.1"
                     /translation="MKISLYILILISILAVSTPVRSQEMIITDDQNYVTPAAGAILDV
                     HSTTRGFIVPRMTTQQRTTLGLLPPADGVVVFDTDLDLFWYWSDGSWVKMATGDPNLE
                     GVTFGDASNYSTFESDGTLLMIGNATVWEDLRISIVLRSSGGTSPVFQQLQGPLYAYK
                     FSGTALNEIFFEMQMPHSWKEGSTVYPHVHWASAGSSTSGVTWGMDYEWKDIGEAFTG
                     TTSNINSTAAPGGLKVHNISDLGSSGITESDKGISSMIM"
     gene            complement(871..>1904)
                     /locus_tag="PKM34_11365"
     CDS             complement(871..>1904)
                     /locus_tag="PKM34_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84236.1"
                     /translation="VNFWGAHNSTIEKVKFYCGDVGTSGQCQDLTINSTDAGRIQQRF
                     VYGYPFDRPWTFWKAGTTISTNPACESFPWTSLPVGTDVTMNMYGLVTGDFNRSFNPV
                     MTKAASSTLSLVYQGNREINFEQEFDLPVRIVESATIGAISLIFNFPSNLVSVTDVVM
                     NGAGGMLDWAVDGDELRIGWNAANPMYLQSNAELLVLRLKTVDGFKSTDVVTISLAAD
                     PLNELADANFEVIGNAVLAVETINGNALGVTEPTDDSGLFFSNYPNPFGHSTKISYTL
                     PADGQVKISISHFTGVHLMTLVNEYQTAGTYEITMDSPDMPTGVYIATISFRTGNNIV
                     MKTHKIIKR"
BASE COUNT          472 a          463 c          468 g          501 t
ORIGIN      
        1 catgatcata ctggaaatac ctttgtctga ttcggtgatt cccgaagagc ccagatctga
       61 aatgttatgg acctttaaac cgccaggagc agcagtggaa tttatattgg aagtagttcc
      121 ggtgaatgct tcaccgatat ccttccattc atagtccatg ccccaggtaa ccccggatgt
      181 ggatgatcct gccgaagccc aatgcacatg gggataaacg gtggagcctt ctttccatga
      241 atgaggcatt tgcatctcaa aaaatatttc gttcaaggct gtgcccgaaa acttgtaagc
      301 atacaatggg ccctgtaact gctgaaatac agggctggtt ccaccggaag acctcagcac
      361 gattgaaatc cggagatctt cccagacggt ggcatttccg atcatcagca gcgtgccgtc
      421 cgattcaaat gtggaatagt tagaagcatc cccgaaggtg accccttcca ggttcgggtc
      481 accggtggcc atctttaccc acgagccatc cgaccagtac cagaataaat caaggtcggt
      541 atcgaatacc accacgccat cggcgggagg caacagccct aaggtagtcc gctgctgcgt
      601 cgtcattcgc ggaacaataa atcctctggt ggtggaatga acatcgagaa tggctccggc
      661 ggcaggggta acataattct ggtcatcggt tatgatcatc tcctggcttc tgaccggggt
      721 tgataccgca agtatcgaaa tgaggattaa tatgtagaga ctgattttca tagactttgg
      781 tttaaaactt tccttgcatt cttagccgga ggtaaaaccg gccgcccatc ctcgcgggcg
      841 accggtgtga attaatgaaa aaagcatcat ttatcttttg atgattttat gggttttcat
      901 cacgatattg tttcctgttc tgaacgaaat cgttgcgatg tatactcctg tgggcatatc
      961 gggtgaatcc attgttattt cgtaagtgcc tgcggtttga tactcgttga ccagggtcat
     1021 caaatgaaca ccggtaaagt ggctgatgct gatttttact tgtccgtcgg ccggcagcgt
     1081 ataagatatc ttagtcgaat ggccgaaggg attcggataa ttgctgaaga acaatccgct
     1141 gtcatccgtc ggttcagtta ccccaagggc attcccgtta atggtctcaa cagccagcac
     1201 tgcatttccg atcacttcga agtttgcgtc tgccagttca ttcagcggat ctgccgccag
     1261 tgaaatagtc accacatccg tggatttgaa tccgtcgact gttttcaacc gtaaaaccag
     1321 taattcggca ttggattgca ggtacatagg gttggccgca ttccagccta tacggagttc
     1381 atcgccatcg acggcccaat ccagcatgcc accggctccg ttcatgacga catcggttac
     1441 gctgaccagg tttgaaggga agttaaagat cagcgatatg gcgccaattg tagcagattc
     1501 gacaattctt acaggcagat cgaattcctg ttcaaaattg atttcccggt ttccctggta
     1561 gaccagcgac agtgttgaac tggctgcctt tgtcatcacc gggttgaaac tgcgattgaa
     1621 atctccggtc acaaggccgt acatgttcat ggtgacatcg gttccgaccg gcaggcttgt
     1681 ccagggaaac gattcacacg ccgggtttgt ggagatagta gttcctgctt tccagaaggt
     1741 ccagggcctg tcgaaaggat acccgtaaac gaaccgctgc tgaatacggc ctgcatcggt
     1801 gctgttgatg gttaaatcct ggcactggcc tgatgttcct acatctccgc aatagaattt
     1861 aactttttca atagtactgt tatgagcgcc ccagaaattg acat
//
LOCUS       DAOQXS010000482         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_73950_length_1867_cov_2.807395, whole genome shotgun sequence.
ACCESSION   DAOQXS010000482 DAOQXS010000000
VERSION     DAOQXS010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73950_length_1867_cov_2.807395"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1007
                     /locus_tag="PKM34_11370"
     CDS             6..1007
                     /locus_tag="PKM34_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ84237.1"
                     /translation="MSLLTVSGSPHIHSDQPVPKIMYGVVIAMIPAMLVSFYFFGLGA
                     AKVMLTSILACLFFEWVIQKYLLRGPLTINDGSALVTGVLLAFNVPSSLPVWMTITGA
                     FVAIAVAKMSFGGLGQNPFNPALVGRVFLLMSFPVQMTTWPIPKPLFGGTAVTDAISG
                     PTTLGIVKEGLDAGKTVSELLPQLPTYAGELLGNQGGSLGEVSAIALLIGAAWMFWKK
                     IITWHIPVSYILAVVIFSGIFWLINPEQYLDPVFHLITGGMMLGVFYMATDMVSSPIN
                     TKGMLIFGAGCGILTMIIRLWGGYPEGVSFAILIMNAFTPLINRSFKPRRFGVVKPS"
     gene            1019..1591
                     /locus_tag="PKM34_11375"
     CDS             1019..1591
                     /locus_tag="PKM34_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNQ84238.1"
                     /translation="MAKKESSLKNMIISLVSISFIASLALGGVYLVTKDPIALAARAK
                     QENAIKEVVPEFDSLHSFKIMPDNGKDSITINEAYKAGTLVGTAVGTYSNKGYDATQI
                     QIMVGFLPDGSINNISVVQQKETPGLGTKMKEPKFKDQFNKKNPASYSLKVKKDGGQV
                     DAITAATISSRAFCDAVDRAYRNYQKKGGN"
     gene            1593..>1867
                     /locus_tag="PKM34_11380"
     CDS             1593..>1867
                     /locus_tag="PKM34_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNQ84239.1"
                     /translation="MNQLKNFTKGFIRENPILVLLLGTCPTLAVTTSAINGLGMGLAT
                     TFVLVGSNIVVSAVKSTIPDKVRIPSFIVIIASFVTIVQLVMQAYTPS"
BASE COUNT          459 a          476 c          491 g          441 t
ORIGIN      
        1 aagatatgag cttactaacc gtatcaggat caccgcatat ccatagcgac cagccggttc
       61 cgaaaatcat gtacggcgtg gtgatcgcca tgatcccggc catgctggtg tcgttctatt
      121 ttttcggcct gggagccgca aaggtcatgc tgacctcgat cctggcctgc ctgttcttcg
      181 aatgggtcat ccagaaatac ctgctccggg gcccgctcac gatcaacgac ggctctgccc
      241 tggtgacagg cgttctgctc gcttttaacg tgccgagcag cctcccggtc tggatgacga
      301 tcacgggcgc tttcgtagcc atcgcagtag cgaaaatgag ctttggcggg ctgggccaga
      361 acccgttcaa tccggcactg gtcggacgtg tcttcctgct gatgtcgttt ccggtgcaga
      421 tgacaacgtg gcccattccc aagccgcttt ttggcgggac agccgtcacg gatgccatct
      481 caggccctac caccctggga atcgtcaagg aagggctgga tgccggcaaa accgtgtcgg
      541 aactgctgcc gcaattaccc acctatgccg gcgaactgct gggaaaccag ggaggatcgc
      601 tcggtgaggt ttccgctatc gccttgctga tcggagcagc ctggatgttc tggaaaaaga
      661 tcatcacctg gcacatcccg gtgtcgtata ttcttgcggt ggtgatcttc tccggcattt
      721 tctggcttat caacccggag cagtatctcg atccggtgtt tcacctgatc acgggcggca
      781 tgatgctcgg tgttttttac atggctaccg atatggtctc atctcccatc aataccaaag
      841 gcatgctgat cttcggagcg ggatgcggaa tactgaccat gatcatcagg ctttggggag
      901 gctatccgga aggagtctcc ttcgccatcc tcatcatgaa tgcgtttacg ccactgatca
      961 acaggagctt taaaccgaga aggttcggag tggtcaaacc cagctaaatc acactatcat
     1021 ggctaagaaa gaatcatcgt taaaaaatat gatcatctcc ctggtatcga tttcctttat
     1081 cgcatcgctg gcgctgggag gtgtatatct ggttacaaaa gatcctatcg cactggcagc
     1141 cagggccaag caggaaaacg ccatcaaaga ggttgtccct gaatttgata gcctgcattc
     1201 cttcaaaatc atgccggata acggcaaaga ttcaattacc atcaacgaag cgtataaggc
     1261 gggaacgctg gtgggtacgg ctgtcggaac ctattccaac aaaggatacg atgctacgca
     1321 gatccagatc atggtcggtt ttcttccgga tggatcaata aataacatat ctgttgtaca
     1381 gcaaaaagag acgcccgggc tggggacgaa aatgaaggaa ccgaagttca aggaccagtt
     1441 caataagaag aatcccgctt catatagctt aaaagtaaag aaagacgggg gacaggttga
     1501 tgccatcacc gctgccacga tcagttcaag ggcattctgc gacgcggtcg acagggccta
     1561 caggaattat cagaagaaag gagggaatta aaatgaacca gctaaaaaat ttcacgaaag
     1621 gatttataag ggagaacccg atcctggtat tactcctggg tacctgcccg acgctggcgg
     1681 tgaccacctc ggcaataaac ggactgggga tggggcttgc aaccacattc gtgctggtag
     1741 gttcgaatat tgttgtgtct gccgttaagt cgaccattcc ggataaagtc aggatacctt
     1801 cgttcatcgt gatcattgct tcctttgtaa ccattgtgca attggtgatg caggcgtaca
     1861 caccttc
//
LOCUS       DAOQXS010000483         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_74939_length_1852_cov_3.567056, whole genome shotgun sequence.
ACCESSION   DAOQXS010000483 DAOQXS010000000
VERSION     DAOQXS010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74939_length_1852_cov_3.567056"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="rsxC"
                     /locus_tag="PKM34_11385"
     CDS             complement(<1..1310)
                     /gene="rsxC"
                     /locus_tag="PKM34_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNQ84240.1"
                     /translation="MCALKTFRNGGVHPPENKLSVTSAIEVFPVPRQAVIPVNQNLGA
                     PATPLVNKGEAVKVGQLIAKGESFISSNVHSSVSGKVLKIDEMIDQTGFRKKAIVISV
                     DGDEWDPSIDRSPMLKKEITLSKEDIVKKIHEMGIVGLGGATFPSHVKLMVPEGKKPE
                     YLIINGVECEPYLTADHRLMLEKTAEIIVGVRILMKGLGVEKAIIGIENNKPDAIRKM
                     AEAASEYPQILVQGLKVKYPQGGEKQLINALLGREVPSGKLPIEVGCLVNNVGTAFAV
                     YEAVQKNKPLIERIVTVTGKTIARPSNYLVRIGTPVQELLLSAQADLDRTGKVVSGGP
                     MMGKALNSLEVPVVKGTSGVLLFAEEHSSRIQLRACIRCGRCVGVCPMGLEPVLLAQY
                     SARSMWDNAESNLIMDCMECGSCHYTCPSGRPLLDYIRMGKNKVG"
     gene            complement(1322..>1852)
                     /locus_tag="PKM34_11390"
     CDS             complement(1322..>1852)
                     /locus_tag="PKM34_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ84241.1"
                     /translation="YGCLGLGDCVAACIFDAIHMDPETGLPVVNDKCTACGACVKACP
                     RNIIELRFKGKKDRRIYVGCVNKEKGGPARKNCTVACIGCGKCVEACAYEAITLVDNL
                     AYIDFVKCRLCRKCAPVCPTDAILEINFPLKKDKEAEPAGGKKATVETSTDSSPADAD
                     KKAPAEGDGLIISENK"
BASE COUNT          403 a          519 c          453 g          477 t
ORIGIN      
        1 ccgactttgt tcttgcccat ccggatataa tccagcaggg gcctgccgga gggacaggta
       61 taatggcagg aaccgcactc catgcagtcc atgatcaaat tggattcggc attgtcccac
      121 atactccggg cggaatactg tgccagcagc accggttcca ggcccatcgg gcagacgccg
      181 acgcagcggc cgcaccggat acaggcacgt agctggatcc tggaagagtg ctcttcagca
      241 aacagcaaaa cgccggaggt gccttttact acaggcacct caagagagtt cagggctttc
      301 cccatcatcg gtccgccgct gaccactttg cctgtgcggt cgagatcggc ctgggccgac
      361 agtagaagct cctgcaccgg ggtaccgatc cgtaccaggt aattggaagg ccgggctata
      421 gtcttcccgg tgaccgtcac aatccgctcg atcaggggtt tgttcttctg aacggcttca
      481 tagacggcaa atgctgttcc gacattattg accaaacatc ctacctcgat cggaagctta
      541 cccgaaggaa cttcacggcc aagaagtgca ttgatcagtt gtttttcccc gccctgcgga
      601 tatttcactt tcagcccctg gaccaggatt tgcggatatt cggaggcagc ctccgccatt
      661 ttccggatag catcgggctt attattctcg ataccgatga tagctttttc gacgcctaat
      721 cctttcatca ggatcctgac acccacaatg atttcggccg ttttttccag catcagccga
      781 tggtcggcgg tcaggtaagg ttcgcattcc acgccgttaa tgatcaggta ttccggcttt
      841 ttcccttcgg gtaccatgag cttgacatgt gagggaaaag ttgcccctcc caggccgacg
      901 atacccattt cgtgaatctt ttttacaatg tcctccttcg acagcgtgat ctcttttttc
      961 aacatcgggc tccggtcgat cgatggatcc cactcatccc catcaacgct gatcacgatc
     1021 gcttttttcc tgaacccggt ctgatcgatc atttcatcga tcttcaagac cttaccggaa
     1081 accgatgagt ggacattgga ggagataaag gattcgcctt tggcgatcag ttgccccacc
     1141 ttaacggctt cgcctttgtt caccaacggt gtggcagggg ctccaaggtt ctggtttacc
     1201 gggatgacgg cctgccttgg tacaggaaat acctcgatgg cactcgttac tgacagttta
     1261 ttttcaggcg gatgaactcc tccattacgg aaagttttca atgcacacat cctacagata
     1321 tttatttatt ttcagaaata ataagaccat caccttcagc gggagctttt ttatcagcat
     1381 ccgccggaga tgagtccgtg gacgtctcta cagtagcttt tttaccgccg gctggttcgg
     1441 cttctttgtc ttttttaagg ggaaaattta tctccaggat ggcatctgtc gggcagacag
     1501 gggcgcattt cctgcaaagg cggcatttga cgaaatcgat gtatgccagg ttatcaacca
     1561 gtgtaatggc ttcataagcg caggcttcca cacattttcc gcatccgatg caagccacgg
     1621 tacagttctt ccgggccggg cctccttttt ccttattcac acagccgaca taaatccggc
     1681 ggtctttttt gcctttgaag cgcaactcga tgatattcct cgggcaggct ttcacgcatg
     1741 cgccgcaggc tgtacacttg tcgttaacaa ccggcaggcc ggtttccggg tccatatgaa
     1801 tggcatcaaa gatgcaggca gccacacagt ctcccaggcc gaggcagcca ta
//
LOCUS       DAOQXS010000484         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_76276_length_1832_cov_2.826674, whole genome shotgun sequence.
ACCESSION   DAOQXS010000484 DAOQXS010000000
VERSION     DAOQXS010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76276_length_1832_cov_2.826674"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKM34_11395"
     CDS             <1..482
                     /locus_tag="PKM34_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84242.1"
                     /translation="NLGADYQATTVNQTTEPPAGWYWQFNRQQGYKHDGSVRTPSTAW
                     ITDITENYDWQPENDPCYLLLGTGWRLPTNTEWTNADINGEWDNANEAFASVLKLHMA
                     GVLTDATGNLAARGTDGDCWSSTQNSTTQSKMLHYSTSNCQIQNSNKEFGLNVRCIK"
     gene            complement(566..1753)
                     /locus_tag="PKM34_11400"
     CDS             complement(566..1753)
                     /locus_tag="PKM34_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNQ84243.1"
                     /translation="MPTQRDLFFRYLGLPSLNAPALEISRAAGVFLYGPDGKDYVDLV
                     SGVAVSNLGHQHPDIVNAVREQAGKHLHLMVYGDLVQSPQVLLAEKLASCLPSALRSV
                     YFVTSGSEAIEGALKLAKRYTGRTGVVAFHKAYHGGTHGALSICGDERLKTAFRPLLP
                     DITHIKFNRFEDLDQITDKTACVVVETIQAEAGIVLPAAGFLEALRAKCTETGALLVI
                     DDIQMGMGRTGKLFSFEHCGIVPDILCLAKAFGGGMPLGAFISSKYIMQSLAHDPELG
                     HITTFGGHPVSCTAALASLNYLVNTGIFEAAEQKAQMFIESLAGHPAIKTIRHKGLMI
                     GIDLESIEMNQKLMKAFHENGLIADSFLFRPQAFRIAPPLIITEEEIEMTCERIIASL
                     NRL"
BASE COUNT          475 a          454 c          463 g          440 t
ORIGIN      
        1 agaacttagg cgcagattac caggctacca ctgttaacca gaccactgag cctccggccg
       61 ggtggtactg gcaatttaac cgacagcagg gatataaaca tgatggcagt gtgcggacac
      121 ccagcacggc atggataacc gatataaccg aaaactatga ttggcagcct gaaaatgatc
      181 catgctattt attgttgggg acaggttggc gacttcctac aaatacggaa tggaccaacg
      241 ctgatattaa tggtgagtgg gataatgcca atgaagcatt tgcaagtgtc ctgaaacttc
      301 atatggcagg tgtattgact gacgctacgg gcaacctggc tgccaggggt acggatggcg
      361 attgctggag cagtacgcaa aattcaacca ctcaaagtaa aatgttgcat tacagcacgt
      421 caaattgcca gatacaaaac tcaaacaagg agtttggact gaatgtgagg tgtataaaat
      481 aacccggatg ggttcatcga aaaagtcgat attctgccga aaagtatatc gaaaaataag
      541 tatgcagtaa aaagaatctt cgcttctaca ggcgattcaa actggcgatg atcctctcgc
      601 aggtcatttc aatttcttcc tcggttatga tcagcggggg cgctatccta aaggcctgcg
      661 gccggaaaag gaatgaatcg gcaatcaatc cgttttcatg gaatgccttc atcagttttt
      721 gattcatctc aatgctttcc aggtcgatcc cgatcattaa ccctttatgc cggatagtct
      781 taatggccgg atgacctgca agactttcga tgaacatttg ggctttctgc tccgctgctt
      841 caaatatgcc tgtatttacc aggtaattca acgaggccag cgcagcagta cagctcaccg
      901 ggtggccgcc aaacgtagta atgtgcccga gttcgggatc atgggcaagg ctttgcatga
      961 tgtactttga cgagataaat gcacccagcg gcatgccgcc tccgaaagcc ttggccaggc
     1021 ataaaatatc tggaactatc ccgcaatgct caaagctgaa taatttcccg gtccgtccca
     1081 tccccatctg gatatcatct attaccagca atgcgcctgt ttctgtgcat tttgcgcgca
     1141 aagcttcgag aaaaccggcg gcgggtagga cgatcccggc ttcggcctgg atcgtctcca
     1201 ccaccacgca ggcggttttg tctgttattt ggtcaagatc ttcgaatctg ttgaatttaa
     1261 tgtgggtaat gtcgggaagc agcggccgga aggccgtttt cagcctttca tcaccgcaaa
     1321 tgctcaaagc gccgtgcgtt cctccatggt atgccttatg aaacgctacg acaccggtcc
     1381 gcccggtata acgctttgcc agtttcaaag ccccttcgat ggcttcgctg cccgatgtaa
     1441 cgaaatatac ggaccgaagg gcagagggga ggcacgaagc cagtttttca gccagcagca
     1501 cctgaggcga ttgtaccagg tcgccatata ccatgagatg taaatgtttt ccggcctgtt
     1561 cccttacagc attaacaata tcggggtgct ggtgccccag gttgctcaca gctacgccag
     1621 aaaccaggtc gacgtagtcc ttaccgtcag ggccatacag gaaaacgccg gcggcacggc
     1681 tgatctccag cgccggagca ttcagcgacg gtaatccgag gtagcggaag aacagatcgc
     1741 gttgtgtcgg catcttatgg caaaatttct gtcgtttccg tgtagatcac attgtgcatg
     1801 gccagatcgt gcagcataaa ttcgacgcac tg
//
LOCUS       DAOQXS010000485         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_76460_length_1829_cov_3.540023, whole genome shotgun sequence.
ACCESSION   DAOQXS010000485 DAOQXS010000000
VERSION     DAOQXS010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76460_length_1829_cov_3.540023"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKM34_11405"
     CDS             complement(<1..285)
                     /locus_tag="PKM34_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84244.1"
                     /translation="MKRWILLALMIFATLFVRSQEMTDSHINKSDREGRRQGLWKVYD
                     EVGNLKYTGDYLDGKPIGTFTYYYPEGEVKALVRHSEGRGAAYVKNYHRNG"
     gene            355..975
                     /gene="recR"
                     /locus_tag="PKM34_11410"
     CDS             355..975
                     /gene="recR"
                     /locus_tag="PKM34_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNQ84245.1"
                     /translation="MNYSSKLLESAVNELSRLPGIGRKTALRLALHLVRQDEEAVVAF
                     SGAVLKLRQEIQRCSQCHNISDNEICDICANIKRDHQLVCVVQDIRDVMAIENTGQYN
                     GVYHVLNGIISPIDGIGPEDLTINSLLEKISREPVREVILALPTTVEGDTTNYYLSRK
                     LAPFQIILTAIARGIAIGDDIEFADEITLGRSIINRVPFTIPGSGV"
     gene            complement(985..1227)
                     /locus_tag="PKM34_11415"
     CDS             complement(985..1227)
                     /locus_tag="PKM34_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84246.1"
                     /translation="MPVVKSRKKPRKKVRYREVKLKLSRSQQKSLANYCSARGTTPTK
                     LIKKMIRPFLSNYSRAVPEELYVTANQLDLFGQENN"
     gene            complement(1300..>1829)
                     /locus_tag="PKM34_11420"
     CDS             complement(1300..>1829)
                     /locus_tag="PKM34_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ84247.1"
                     /translation="TVSNLSSTRALKDVTERYGGQYFASAVGEVNVVEMMKKTRAVIG
                     GEGNGGVIYPDLHYGRDALVGIALFLSHLATSGTTCSDLRKTYPDYFISKNKVVLKPG
                     SNPSDILVHIKKTYKDFPMNDSDGIRIDFPEGWVHIRQSNTEPIMRIYSEAAAMSVAE
                     QLAARIRMDISQWIC"
BASE COUNT          481 a          435 c          412 g          501 t
ORIGIN      
        1 cccgttgcga tgatagttct tcacataagc agctccccta ccctcactgt gcctgaccag
       61 cgctttgact tctccttccg ggtaataata ggtaaaggtg ccaatgggtt tgccatccaa
      121 ataatcacct gtatatttaa gattaccgac ctcatcataa actttccaca atccctgccg
      181 ccggccttca cggtcggatt tattgatatg ggaatctgtc atctcctggg agcggacaaa
      241 aagggtggca aatatcatca gggctaaaag tatccagcgt ttcatggttc caacaattga
      301 ttaattttgt cagaaaacgt aaaattacca tttttattaa acaccggaac taagttgaac
      361 tattcgtcga aattactcga aagtgctgtc aatgaattgt cgcggcttcc cggaattggc
      421 agaaaaacag ctctccggct ggcgcttcac ctggtccgcc aggatgaaga ggccgtggtg
      481 gcgttcagtg gagccgtgct caaactcagg caggaaatac aacggtgctc acaatgccat
      541 aatatctccg acaacgaaat ctgcgatatt tgtgctaata taaaacgcga ccaccaactg
      601 gtatgtgtgg tgcaggatat cagggatgtg atggccattg aaaataccgg ccagtataac
      661 ggcgtttatc atgtgctgaa cgggatcatt tcccctattg atggaatcgg gccggaggat
      721 ctgacaatca attcacttct ggagaaaatt tcgcgggaac cggtcaggga ggtcattctg
      781 gccttgccca caaccgtcga aggcgatacg accaattatt acctgtcgag aaagttggcc
      841 ccatttcaga ttatactgac tgcgattgcc cgcggaatcg ccattggcga cgatattgaa
      901 tttgccgatg aaataactct gggacgatcc ataataaaca gggtcccctt taccataccc
      961 ggttcagggg tttaaaatag accattaatt attttcctgc ccgaacagat cgagctgatt
     1021 ggccgtcaca tacaattctt cgggcactgc cctggaatag ttggagagaa acggcctgat
     1081 catctttttg atgagtttag tgggtgtagt gccccgtgct gaacaataat tggccagcga
     1141 tttttgctgg ctcctggaaa gtttaagttt gacttccctg taccgcactt ttttgcgggg
     1201 ctttttcctc gatttgacta caggcataga ttccgtgatt ttcaggattc taaaatagaa
     1261 ttatttaaag aaaggatccg ttgacagaac cgaaatttat taacaaatcc attgtgagat
     1321 atccattctg atgcgggcgg ccagttgctc ggcaacgctc atggcagccg cctcggagta
     1381 tatccgcatg atcggttcgg tattggattg gcggatatgt acccatcctt ccgggaaatc
     1441 aatcctgatt ccatccgaat cattcatggg gaaatccttg tatgtctttt ttatgtgaac
     1501 gaggatatcg gaaggattgc tgccgggctt cagaacaact ttattttttg aaatgaaata
     1561 atccggatat gtctttcgca aatctgaaca ggtagtcccg gacgtagcca gatggctcag
     1621 gaacaatgct attccaacca gcgcatcgcg cccgtaatgc aaatccggat agatcacacc
     1681 gccgtttccc tcaccgccta tcaccgcccg ggtttttttc atcatttcaa ccacattgac
     1741 ctctcctact gccgatgcaa aatactgacc gccatatctt tcggttacat cttttaaagc
     1801 ccgcgttgaa gataaatttg agacagtat
//
LOCUS       DAOQXS010000486         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_78387_length_1802_cov_2.599313, whole genome shotgun sequence.
ACCESSION   DAOQXS010000486 DAOQXS010000000
VERSION     DAOQXS010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78387_length_1802_cov_2.599313"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="secA"
                     /locus_tag="PKM34_11425"
     CDS             <1..972
                     /gene="secA"
                     /locus_tag="PKM34_11425"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ84248.1"
                     /translation="EENNFGIRKRLLEYDDVMNSQREVIYKKRRHALFGDRLAVDISN
                     MIYDVGEQMINQYLEEKDFEGFKLALLRTMSIESPFSADEFFSMRTETMADQLYDAAY
                     KHYVEKSKRIAELAYPVIKDVYENQTQYENIAIPITDGIKTMNVVANLKRSYENGGKE
                     IILSIEKGITLAMIDEAWKEHLREMDDLKQSVQNATYEQKDPLLIYKFEAFELFKKMV
                     ARINEEITSFLIRGKLPLQDASSVKEARAPRGIDRSRMREERKDLLAQSHSDTQANRP
                     NEPMKRDIPKIGRNDKVKVQYGDGRIIVAKFKAIEHDLNRGNCQIIN"
     gene            981..1685
                     /gene="pyrH"
                     /locus_tag="PKM34_11430"
     CDS             981..1685
                     /gene="pyrH"
                     /locus_tag="PKM34_11430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ84249.1"
                     /translation="MRYNRILLKLSGESLMGKASYGIDPERLNDYSEEIAAAARQTVQ
                     IAIVIGGGNIFRGMQGSARGFDRQTGDYMGMLATVINSLVLRSSLEEKGVKVKLMSGI
                     YIEGLTEKANGMTAKQYLEDGYVVIFSGGTGNPFFTTDTASALRAVEISADAMFKGTR
                     VDGVYTDDPEKNPEAIKYDHLTFHEAFVKELKIMDLTAFTMCEENKLPVIVFNMNIKG
                     NLIKLLNGEKIGTRIE"
BASE COUNT          549 a          406 c          444 g          403 t
ORIGIN      
        1 gaggagaaca acttcggcat caggaagagg ctgctggaat acgacgacgt gatgaactcc
       61 cagcgggaag tcatctacaa gaaacgccgc catgcgcttt ttggcgacag gctcgccgtc
      121 gatatatcca atatgatcta cgatgtcggc gaacaaatga tcaaccagta cctggaagaa
      181 aaagatttcg aaggctttaa actggcgctg ctccggacca tgtcgatcga atcgccgttc
      241 agtgcggatg aattcttttc catgcggacc gaaacaatgg ccgaccagct ttatgatgcc
      301 gcatacaagc attacgtaga gaaatcgaaa cggatcgccg aactggccta cccggtcatc
      361 aaggatgtgt acgaaaatca aacccagtac gaaaatatcg ccattccgat caccgacggg
      421 atcaagacga tgaatgttgt ggccaatctg aagcgatcct acgaaaacgg gggcaaagag
      481 attatcctct ccatcgagaa aggaattacc ctggccatga tcgatgaagc ctggaaagag
      541 caccttcgcg aaatggacga cctgaagcag tcggtccaga atgctaccta tgagcagaaa
      601 gatccattgc tgatatacaa gtttgaagct ttcgaattgt ttaaaaagat ggtcgcccgc
      661 atcaacgaag agatcacctc cttcctgatc cgtggtaaac ttccgctgca ggatgcctct
      721 tcggttaagg aagcccgggc gccaagaggc atcgaccgtt caaggatgcg ggaggagaga
      781 aaagacctgc tggcccagtc gcatagcgat acccaggcaa accggccgaa tgagcccatg
      841 aaacgcgata tccccaaaat cggcaggaat gataaagtga aagtccagta cggcgacggt
      901 cggatcattg tagctaagtt caaggccatc gaacacgacc taaaccgggg caattgtcag
      961 attatcaact aaataaaaag atgagataca atcgaattct tttaaagcta agtggtgaat
     1021 ccctcatggg aaaagcatcc tatggtattg atcccgagcg gttgaatgat tattcggaag
     1081 aaattgctgc tgcagcacgg caaaccgttc agatagctat tgtaattggc ggcgggaata
     1141 tcttccgcgg tatgcagggc agcgcccggg gattcgaccg ccagaccggc gattatatgg
     1201 gcatgctggc aactgttatc aatagcctgg tcctgcgatc ttcgctggaa gaaaaaggcg
     1261 ttaaggtcaa actgatgtcg ggtatttata tcgaaggatt gacggaaaag gccaatggga
     1321 tgaccgcaaa gcaatacctg gaggatggct atgtggttat tttcagcgga ggtaccggga
     1381 atcccttttt taccaccgat accgcctcgg cgcttagagc agtggagatt tcagccgatg
     1441 ccatgttcaa gggtacacgc gttgatgggg tttataccga cgatcccgag aaaaacccgg
     1501 aagcgataaa atatgaccac ctgacttttc atgaagcgtt cgttaaagaa ttgaagatca
     1561 tggaccttac tgcatttaca atgtgtgagg aaaataagct tcccgtcatt gtttttaata
     1621 tgaatattaa gggtaacctg atcaaactcc tcaacggaga aaaaatcgga acacggatcg
     1681 aatagctata aacccggttt agtcatattt tgagctattt tatcaaaaaa aattaagcat
     1741 taaaaattaa aaattaagaa ttaaccaata gtttcgccac ttccttccac gaatgtacct
     1801 ca
//
LOCUS       DAOQXS010000487         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_79560_length_1786_cov_2.307337, whole genome shotgun sequence.
ACCESSION   DAOQXS010000487 DAOQXS010000000
VERSION     DAOQXS010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79560_length_1786_cov_2.307337"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..>1786
                     /locus_tag="PKM34_11435"
     CDS             547..>1786
                     /locus_tag="PKM34_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ84250.1"
                     /translation="MASLWIDERDIKFQLNEVFNIGETILGKGRYADHDLEMCNMVLD
                     QAQKFAEADIAPTYPDEVHRKPVEAVFKDGKVYAPEAYHRLYKLYCEGGWMTTSDSPE
                     VGGQSFPFIIGAAVANMFLAANQAFLMYPGLTHGAARLMEEHFRHPLRDVVIEKMYSG
                     QWAGTMCLTEPGSGSDLGILKTTAKKNADGTYSIVGTKCFISAGDHDLTENIIHPVLA
                     RIEGDPEGTKGISIFMVPKIRFNEKGELGEPNDVLCGNIESKMGIHGNATCTLNFGTD
                     GKCIGYLMGEEREGMKIMFQMMNEERQGVGMMGVSLAMAAYLHALDYAKQRIQGSNII
                     AVALKDPNAKAVPIIQHPDVRRMLMKQKSITEGIRALAFLCYYSMDREFNAGLENNEQ
                     EKDYWHGMIEMLTPIVKSYCT"
BASE COUNT          485 a          411 c          449 g          441 t
ORIGIN      
        1 gcagcggtct gcaaaaccgt tattctccgg ttcaaatccg gatgccgcct ccagccatca
       61 cgaggagctt tctttccgga ggctcctttt tcaaaactga aataatccaa aattgactga
      121 tagagtgcac ctcacacgtg ttttgttctg aagcgctcgt attagcggtt tcagcagaac
      181 actttttgtg taaaaggcac gaatttttaa attgttccga caagaccaaa atcttcttga
      241 cacgccacta cctgccgcat atactccacg aaaaaaagga attccttatc aaattattcc
      301 atgagtcaaa acatcggcat gcccttgagc agttaaaggc cgtttggtga atatcggtaa
      361 aaagtgaaat gttcgttcat ccctgtttga tcctcgggaa agcatttcca gctggtatat
      421 aagactttat ttttacaact gaatatggat tatggtcatt ttttattacg cgaatattga
      481 ctaacagtct gataatacga tataggtaag caatatcgta aataaattta agttaaggag
      541 gtttttatgg caagtttatg gattgatgag agggacataa aattccagct gaatgaagtt
      601 ttcaatatcg gggaaaccat tctgggcaaa ggtcgctatg ccgatcacga tctggaaatg
      661 tgcaacatgg tgcttgacca ggcgcagaaa tttgctgaag cggacatcgc tcccacttat
      721 ccggatgagg ttcatcgtaa accggtcgag gctgtattca aggatggaaa agtctatgcc
      781 ccggaagcct accaccgcct ctataagctg tactgtgagg ggggatggat gaccacatcc
      841 gattcaccgg aagtcggcgg tcagtctttc ccgtttatca tcggggctgc agtggccaat
      901 atgttcctcg ccgcaaatca ggcctttctt atgtatcccg gcctgaccca cggtgcggcg
      961 cgtttgatgg aagagcactt ccgccacccg ttgcgtgacg tcgtcatcga aaagatgtat
     1021 tcaggtcagt gggcgggcac gatgtgcctg accgaaccgg gatctggctc tgacctgggc
     1081 atcctgaaga ctacggccaa aaaaaatgca gatgggacat attccattgt cgggacaaag
     1141 tgcttcattt cggcaggaga tcacgacctg acagagaata ttattcatcc ggtgctggcc
     1201 cgcatcgaag gagatccgga aggaacgaag ggtatctcca tatttatggt gcccaagatc
     1261 cgcttcaacg aaaagggaga actgggagaa cccaacgacg tgctatgcgg caatatcgaa
     1321 agcaaaatgg gtatccacgg caatgctacc tgcacactga acttcggcac cgacggcaag
     1381 tgtatcggtt atcttatggg cgaagagcgc gagggcatga agatcatgtt ccagatgatg
     1441 aacgaagaac ggcagggtgt gggcatgatg ggcgtgtccc ttgccatggc ggcttacctt
     1501 catgctcttg actatgccaa gcagcgtatc caggggtcga acatcattgc tgtagccctg
     1561 aaagatccga atgcgaaggc cgtaccgatc attcagcatc ctgacgttcg ccggatgctt
     1621 atgaaacaga agagcattac cgaaggcatc cgggcattgg catttttgtg ctactactcc
     1681 atggatcgtg aattcaacgc cggtctggag aacaatgagc aggaaaaaga ttactggcat
     1741 ggcatgatcg aaatgctcac cccgatagtc aagtcatact gcacgg
//
LOCUS       DAOQXS010000488         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_84899_length_1716_cov_3.669476, whole genome shotgun sequence.
ACCESSION   DAOQXS010000488 DAOQXS010000000
VERSION     DAOQXS010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84899_length_1716_cov_3.669476"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKM34_11440"
     CDS             complement(<1..408)
                     /locus_tag="PKM34_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNQ84251.1"
                     /translation="MSAFILSDRYFAPESGHISSYFSQLYMLVGHSSVRYTILDTEKN
                     TFVGLASYTFSPAPKSSTELRAGLERLFTEDDFLARKYPSVTIGIDSERFTLVPFPLF
                     DDIRTREILSFNFRLQENSNVLTDKIAEISAYLV"
     gene            complement(467..1066)
                     /locus_tag="PKM34_11445"
     CDS             complement(467..1066)
                     /locus_tag="PKM34_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84252.1"
                     /translation="MKYTIIKVVLSVIIVVLAYLIYESILRPVRFNKATSAREAKVIA
                     TLTDLRTSQQFYKRQHNKYTASFDTLISFLRNGEIPVVKMIPDPNDTTFTKTINDTLG
                     YVKVADSLFRARPGYNLDSLRYIPFSGGELFEIAASEIDRSGIKVGVYEIKAHYSTFL
                     KGLDRQLVINLIKSKEDIEKYPGLKIGSMEEPSTDGNWE"
     gene            complement(1192..>1716)
                     /locus_tag="PKM34_11450"
     CDS             complement(1192..>1716)
                     /locus_tag="PKM34_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNQ84253.1"
                     /translation="RSVFATGGFLLILGWNSVLKNSPKIMIKLNFPEFEFKREIRDGK
                     ELIFDPVRRRFVALTPEEWVRQHAIRYLTGYKNVPVSLIRIEAVIKLFRTKKRFDLVV
                     SDRNGNALLLAEFKSPAVAVTQSVLDQAVRYNLALKVKYLVLSNGIQHVYCQVGNDPG
                     SLKLLDDLPEFQFL"
BASE COUNT          476 a          375 c          397 g          468 t
ORIGIN      
        1 gaccagataa gccgaaatct ccgcaatctt atcagtcagg acattagaat tttcctggag
       61 cctgaagtta aaggatagaa tttcgcgggt gcggatgtcg tcgaataaag ggaaaggaac
      121 tagtgtgaag cgttccgaat caatgccgat agttactgat gggtatttcc gggccaggaa
      181 gtcgtcttct gtaaaaagtc tctccaggcc ggccctgagc tcagtggatg atttcggcgc
      241 cggactgaag gtataggaag caagaccaac aaatgtattc ttttccgtat ccaggatagt
      301 atatctgacc gacgagtgac cgaccagcat atacagctga ctgaagtaag acgaaatatg
      361 accgctttcc ggagcaaaat acctgtcgga gagaataaat gcactcatag cagtaacgaa
      421 agatcccggt atgatttaat tttccgggat caactccttc ccgggactat tcccagttac
      481 catcggtgga aggttcttcc atcgacccga tcttcagacc gggatacttt tcgatatctt
      541 cctttgattt gataagattt ataacaagct gacgatcaag gcctttgagg aaggttgaat
      601 aatgagcttt tatctcataa actcccactt taatcccgga cctgtcgatt tcgctggcag
      661 cgatctcaaa caattctccg ccagagaaag ggatataacg aagggaatcc aggttgtagc
      721 ccggcctggc gcggaacagc gagtcggcta ctttcacata accgagcgta tcgttgatgg
      781 ttttggtaaa tgtggtatca ttcggatcgg ggatcatttt taccacgggg atttcaccgt
      841 ttctcagaaa actgataagg gtatcgaaac tggcggtgta tttattatgt tgccttttat
      901 aaaactgctg actggtcctg agatcggtga gtgtggcgat taccttggct tcgcgagcgc
      961 tggtagcttt gttaaacctg acgggtctca atatgctttc atagataaga taagccagaa
     1021 ccacgatgat aactgaaagg acaaccttga taatcgtgta tttcatatca aaaaagtttg
     1081 gttttattga aaagcaaatg tataaattat tttctatctc aaacggatat ttattgatta
     1141 aaaataggcg ttttcttcct tttcattcct ttacccggat tgctttaaat tttacagaaa
     1201 ctggaattca ggaagatcat ccagaagttt cagggaaccc ggatcattgc caacctggca
     1261 ataaacatgc tgaatcccgt tcgacaagac caggtatttg actttcagtg ccaggttata
     1321 ccgcaccgcc tggtcgagca cggattgcgt aacggcaact gcaggcgact tgaattcggc
     1381 cagcagcagg gcattgccgt tacggtcgct gacaaccagg tcgaaacgct ttttagtcct
     1441 gaacaacttg ataacagctt cgatgcggat aagtgagaca ggtacgttct tataaccggt
     1501 aaggtaccgg atggcatgtt gcctgaccca ttcctccggt gtcagagcca caaaccgcct
     1561 tctgacggga tcaaagatca actcttttcc atcgcggatt tcgcgtttga attcaaattc
     1621 ggggaaattc agttttatca ttattttcgg ggagttcttc agcacggaat tccatcccaa
     1681 aattaacaaa aatcctcccg ttgcaaaaac ggatcg
//
LOCUS       DAOQXS010000489         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_91478_length_1640_cov_1.652366, whole genome shotgun sequence.
ACCESSION   DAOQXS010000489 DAOQXS010000000
VERSION     DAOQXS010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91478_length_1640_cov_1.652366"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..>1640
                     /locus_tag="PKM34_11455"
     CDS             345..>1640
                     /locus_tag="PKM34_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNQ84254.1"
                     /translation="MIKSRLEELSVDENTRIAGEKMHSIEYISNLYAKNDYRPFWTKA
                     EYVGDAISALAGSYHDGLLTDDYHLQAIIRLHTETVAIQDESEEKISRTADLDLLLTD
                     GIIYYAEHLLFGKTDPVTLENTWNFGFAPIPDLNSESLNHYISDREIIIRLEQLRPDM
                     HLYDTLLNTLAQYRHIMANGGWGTVLAGGKIEPGNSDARIPAIRKRLLATGGLSEKDS
                     TSSTFYDKLLERDIKAFQATHGLEPDGIIGVGTFRELNVPVEDRIASIRVNMERVRWV
                     AGNMPQKFIIVNIAAFWLMMVDQGEIVHYTPVVVGKPLNKTPMFRDKMRYIEFNPTWI
                     QPRSIVKDETVPKLKKDSSYLEKNHMVLLDSKGNLVPTSSLDLKNLSASKFPYLVRQE
                     PGPWNSLGEMKFMFPNKYEIYLHDTPSKSQFSRASRAYSH"
BASE COUNT          475 a          417 c          384 g          364 t
ORIGIN      
        1 gatcgacgcc ctggcttcac tcaaactgga attcccgaaa ctggacaaga aaaagctgga
       61 agagctgaaa gtggcgaggg aagcgctgat gagtgaataa cgaatctttc cttttcatca
      121 acctgactgg tctgacgacg ctgtcaggtt tgacctgtga taacctgtca gagtccgacg
      181 caggaggacc tgacagcgtt ataaaaatga aaattaatgt tcaatgaaaa tatctttgct
      241 ttcctttgcg tgaaatttca aaaatcatgc gccaggctat cattataatc atatcacttt
      301 tctttattac gccagaaaat aaagctgaaa gtaatatttc aggaatgata aaatcaagac
      361 tggaagaatt atcggttgat gaaaatacca ggatagccgg ggaaaagatg cacagcatcg
      421 agtatatctc caatctttat gcaaagaacg actaccggcc cttttggacg aaagcggagt
      481 atgttggtga cgccatttct gccttagcgg gttcttatca cgacggactg ctgacggatg
      541 attaccatct gcaggccatc atccggctgc atactgaaac cgttgccata caggatgaat
      601 cagaagaaaa aatctcccga acagccgacc ttgacctgct gctgaccgat ggcatcatct
      661 actatgccga acacctcctt tttggcaaaa ccgacccggt aaccctcgaa aatacctgga
      721 acttcgggtt cgccccgatc ccggacctga attccgaatc cttaaaccat tatatttccg
      781 acagagaaat tataattagg ctggagcaac tcaggccgga catgcatttg tacgacacac
      841 tgctgaacac actggctcaa taccggcata ttatggccaa tggcgggtgg ggaaccgtcc
      901 tggccggtgg aaagatcgaa ccgggtaaca gtgacgcccg catccctgcc atccgtaaac
      961 ggctgctggc aaccggcggg ctcagcgaaa aagactccac ctcatcgaca ttctatgata
     1021 agctcctgga gagagatatt aaagctttcc aggctacaca tggcctcgaa cctgacggga
     1081 tcatcggtgt ggggaccttc agggagctca acgtgccggt cgaagaccgg atcgcctcca
     1141 tcagggtgaa tatggaacgc gtccggtggg tcgcgggaaa tatgccccaa aaattcatca
     1201 tcgtcaatat cgccgccttc tggctgatga tggtcgacca gggggaaatc gttcattata
     1261 ccccggtggt agtgggaaaa ccactgaaca aaacaccgat gttcagggat aaaatgcgat
     1321 acattgaatt taacccaacg tggatacagc ccaggtctat cgttaaagac gaaaccgtcc
     1381 cgaaactcaa gaaagactct tcgtaccttg aaaagaacca catggtgctg ctcgacagca
     1441 aagggaacct ggtcccgact tcctcgctgg atcttaaaaa cctttccgcg tcaaaattcc
     1501 cctatcttgt caggcaggag cccggtccat ggaattcact cggcgaaatg aagttcatgt
     1561 tccccaacaa atacgaaatt tacctccacg acaccccgtc gaagagccag ttcagcaggg
     1621 caagcagggc ttacagccac
//
LOCUS       DAOQXS010000490         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_92622_length_1627_cov_3.186387, whole genome shotgun sequence.
ACCESSION   DAOQXS010000490 DAOQXS010000000
VERSION     DAOQXS010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92622_length_1627_cov_3.186387"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKM34_11460"
     CDS             complement(<1..1221)
                     /locus_tag="PKM34_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84255.1"
                     /translation="MMKKLLLIFVGLAAMSYLQAQEGWIEKNSNLTVGNGIGQISVGM
                     LDQTALWALPVDATGAIVDGFTRSLDGGQTWYPGTFNAGDGLSQLFAINSTTCWAVFN
                     TGATQGLYKTENAGATWTKKGNAYGSSSFANILHFFNEDDGVAQGDPVGGYYEIYTTS
                     NGGETWTRVPSINIPAPTSGEYGITGNYDAVGDHIWFGTNKGRIFHSADKGLHWTASL
                     TTFGATTVVQPEFSDELNGMAFRSYLDLGLEPTLSITHDGGATWESVNVAGPMYARYI
                     TYVPGTTATFVGSASAEGENGISYTTDGGYTWAAITEGYDFMASVWLDNETGWAGSFA
                     RSSRSVGGMYIYDGPPLEAFEVPGIAFNPSEIVEQAEAGGAESQILTVSNTGGVDLVY
                     SAHVVYVQPAKSAPQ"
     gene            complement(1385..>1627)
                     /locus_tag="PKM34_11465"
     CDS             complement(1385..>1627)
                     /locus_tag="PKM34_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNQ84256.1"
                     /translation="PEIGNVNFSVYPNPVVNDMTITSDSEMNRIEIFNQLGQKVFARD
                     IKEFKYRVNASEFENGVYYIRITAETGVTTQKVTIK"
BASE COUNT          412 a          388 c          395 g          432 t
ORIGIN      
        1 ctgcggagcg cttttagcag gctggacata aactacatga gcgctgtaga ccaggtcaac
       61 tccgccggta tttgatacgg tcaggatttg tgattcggcg ccgcctgctt cggcttgttc
      121 gacgatttca gagggattga aagcgattcc gggtacttca aaagcttcca gcggaggtcc
      181 gtcgtaaatg tacatacctc caacgctgcg gcttgaccgt gcaaatgaac ctgcccaacc
      241 tgtttcgtta tcaagccata ccgatgccat gaaatcatag ccttctgtaa tagccgccca
      301 ggtataacca ccatccgtgg tgtaagatat gccgttttcg ccttctgcac tggctgatcc
      361 gacgaaagtg gccgtcgttc ccggaacata ggtgatatag cgggcataca tcgggccggc
      421 tacattgacc gattcccagg tagcgccacc gtcatgagta atgcttaatg taggctcaag
      481 accgagatcc agataggaac ggaatgccat gccgttcagt tcatccgaga actcgggctg
      541 tacgacggtc gttgcgccga aggtcgtcag cgaagctgtc cagtggagcc ctttgtcagc
      601 agaatggaag atcctccctt tattggtacc gaaccagata tgatcgccca cggcgtcata
      661 gtttccggta ataccgtatt caccgctggt aggtgcgggg atattgattg aaggaacgcg
      721 tgtccaggtc tcaccgccat tgctggtggt ataaatttca taatatccgc ctaccggatc
      781 accctgtgca actccgtcgt cttcgttaaa gaagtgaaga atattggcaa aggatgagct
      841 gccatatgca ttaccttttt tggtccaggt ggccccggcg ttttcggttt tgtaaagtcc
      901 ctgggtggct ccggtgttga acacggccca gcaggttgtg gaattgatgg caaagagttg
      961 cgacagcccg tcgccggcat tgaatgtacc gggataccag gtctggcccc cgtcgagtga
     1021 acgcgtaaag ccatcgacga tagcgccggt agcgtcgaca ggcagagccc acaaggctgt
     1081 ttgatccagc attccgacgg agatctgtcc gataccgtta ccgactgtca ggttggaatt
     1141 tttctcaatc catccttcct gggcctgcaa ataagacatg gctgcaagcc cgacaaaaat
     1201 caatagtaat tttttcatca tattgtttga attagaattt aatgattcaa atttatggat
     1261 aaatctttca atacaaacca acaaaatata aagtcattaa tgttaatggt aaaacaaaaa
     1321 aaagaggctg cctctgaatc aggggcagcc tcttgtctta tggcattaat aatgaaagaa
     1381 atgcttattt aatagttact ttttgagtag taactccggt ttcagcggtg atccggatat
     1441 aatacactcc attctcaaat tcgctggcat tcactctgta tttgaattct ttgatgtctc
     1501 tggcgaacac tttctgtccg agctggttga aaatttcgat cctgttcatt tccgaatcgc
     1561 tggtaatcgt catatcgtta acaaccggat taggataaac cgagaagtta acattaccga
     1621 tttcagg
//
LOCUS       DAOQXS010000491         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_97870_length_1574_cov_3.610928, whole genome shotgun sequence.
ACCESSION   DAOQXS010000491 DAOQXS010000000
VERSION     DAOQXS010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97870_length_1574_cov_3.610928"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1574)
                     /locus_tag="PKM34_11470"
     CDS             complement(<1..>1574)
                     /locus_tag="PKM34_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84257.1"
                     /translation="YDKALRKMKGITPEKLHCTFNLAECYRLMNDPDLAEPYYRQLAE
                     TSYGDTVPVYYLRYATILKTRGDAESARKYFEKYLQTDPGNMEAETGLKSCDWLLENR
                     HKKAQVIVEEITRINTPDDEFGPAYLNPQKNSIIFTSNRVTEDSKNIDQWTGSRFSDL
                     YISAFDGKNWSDPELAEYGGLLNSDVHEGTPSLNGDYSVMYYTRCGKTANEKSFCEIW
                     KTVKTGDQWSKPAPVLTDSVANIGHPAISKDERILIFSSNRPGGTGGKDLWAAKRNSA
                     DEPFGRPELLGPGINTAGDELFPYLYNDTTLLFASNGYEGYGGLDIFISNLRKNKWTK
                     PENLLAPINSSYDDFGIILKKPFEEGCFCSNRNGGTGGDDIYYFYRRILLFTASGQVK
                     DNSTLLSVKGAEVILAGKAGDTVKVMTDKQGLFRFDNSQVKEDVDYELVFRKQGYFSK
                     KETFSTRPYEEDHDFSIDIKIDPIPEKPIVLPDILYELDKWDLAPQYQDSLLQLVRLL
                     KDNETLVIELRSHTDS"
BASE COUNT          365 a          387 c          381 g          441 t
ORIGIN      
        1 gagaatcggt atgagaacgc agctcaatca ccagcgtttc attgtctttc agcaagcgca
       61 cgagttgcag aagtgaatcc tggtattgcg gggcaagatc ccatttgtcg agttcgtaaa
      121 gaatgtcggg cagaacgatg ggtttctcag gaatcggatc gatctttata tcaatcgaaa
      181 aatcgtgatc ctcttcatac ggccgggtgg agaatgtttc cttctttgaa aaatatcctt
      241 gtttacgaaa aaccagttca tagtccacat cttcttttac ctgggaattg tcgaaacgga
      301 ataggccctg cttatcggtc atcactttga ctgtatcacc ggccttacca gcgaggatca
      361 cctctgcacc tttgacggat agcagggtgc tgttgtcttt cacctggcct gatgccgtga
      421 aaagcaatat cctcctgtag aaataataaa tatcgtcgcc gccggtgccg ccattgcgat
      481 tggagcagaa acagccttct tcaaaaggct ttttcaggat gattccgaaa tcatcgtaag
      541 aagaattaat tggtgccaga aggttttccg gcttggtcca tttgtttttt ctgagattag
      601 agataaatat atccagccct ccgtagcctt cataaccatt ggaagcaaac agcagggtcg
      661 tgtcgttata aaggtaaggg aacaactcat cgccggcggt attgatcccg gggcccagca
      721 attccggccg gccgaaaggt tcatcagcgc tgttccgttt ggctgcccat aaatctttgc
      781 cgccagtacc gcccggtctg ttcgacgaga agataagtat cctttcatcc ttacttatgg
      841 ccggatgccc gatgtttgca accgaatccg tgagcaccgg tgcaggcttg ctccactggt
      901 caccggtttt gacggttttc catatttcac agaacgactt ctcgttcgcc gttttaccac
      961 accgggtgta atacatcaca gaataatccc cgtttaggga tggcgtccct tcatggacat
     1021 ccgaattcaa cagtccgcca tattcggcca attccggatc actccagttt tttccgtcaa
     1081 aagcgctgat atacaggtcc gagaatcgcg acccggtcca ctggtcaatg ttttttgagt
     1141 cttccgttac ccggttggaa gtaaagatga tggaattctt ttgcggattt agatatgccg
     1201 gcccgaattc atcgtcgggg gtattgatcc gggtgatctc ttccacgata acctgggcct
     1261 tcttatgacg gttctccaat agccagtcgc atgatttgag ccccgtttcc gcttccatgt
     1321 tcccgggatc ggtttgcaga tatttctcaa aatattttct ggcggattca gcatccccac
     1381 gagttttcag gatcgttgca taccgaagat aataaacagg gacggtatcg ccgtatgagg
     1441 tttctgccag ctgcctgtaa taaggttcgg ccaggtccgg atcgttcatc aggcggtaac
     1501 attcggccag gttaaaagtg cagtgcagtt tttcgggagt gatccccttc atcttgcgga
     1561 gggctttgtc gtag
//
LOCUS       DAOQXS010000492         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_99364_length_1560_cov_2.909635, whole genome shotgun sequence.
ACCESSION   DAOQXS010000492 DAOQXS010000000
VERSION     DAOQXS010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99364_length_1560_cov_2.909635"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..924
                     /locus_tag="PKM34_11475"
     CDS             112..924
                     /locus_tag="PKM34_11475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ84258.1"
                     /translation="MMLTVLLAAAIVYAGICVSFYFFQHLAFFRPERLSASFTYQYPF
                     PFEELNFDMEDGGRINAIYFKVPNTRGVVYYLKGNSKSIKGWGKFAKDFLSNGYDFFM
                     MDYRGFGKSRGKRSQEKVFNDAQFIYQWLAEKYPEDQIVLYGRSMGSGIAARIASWNK
                     PRMLILDSPYFSFYYNVNRYLFFTPLKWLLKYDMRTDQYLKTVSCPVHIIHGTRDRLI
                     NYGQSEKLKALFPEKIVLHPIKDGKHNNLPEFPEFFEVLYDILYVKPAVTST"
     gene            924..>1560
                     /locus_tag="PKM34_11480"
     CDS             924..>1560
                     /locus_tag="PKM34_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ84259.1"
                     /translation="MEISSKPSANFTRLFDLIDYQRARYPNAQALNACINSQWVAESI
                     ETFRHRVDSISGYLLQKNYQLGEKIILIPDIGSADWMAIDLACQQIGLIPVPLHATVS
                     EAELTIILDETQACFCIVTFNQWDRIRNIFEKKTKSPVLYHLSANHAGYFLGEIKGNV
                     SSDVLATIENIKSSIDENDLATIMYTSGSSGEPKGVMLSHRNIVHNIKSVLT"
BASE COUNT          412 a          366 c          360 g          422 t
ORIGIN      
        1 aggaggaggt cacaggaact taagagactt ccccttgtat cacgaaaagc tggcggagat
       61 tcttccgcag gatccggatt agtaattcac tcattcttca taaattaagc catgatgttg
      121 accgtgctcc ttgcggctgc tattgtatac gccggcatct gtgtctcctt ttattttttt
      181 cagcacctgg ccttcttccg gccggagcgg ttgtctgcct cctttaccta tcagtatcct
      241 tttccattcg aggaacttaa ttttgacatg gaagatggcg gcagaatcaa cgccatttat
      301 ttcaaggtgc ccaacacacg gggtgtggtc tattacctga agggcaattc caaaagcatc
      361 aagggatggg gaaaatttgc caaagatttt ctcagcaatg gatacgattt ctttatgatg
      421 gattaccggg ggtttggaaa gagccgaggg aaacgcagcc aggaaaaagt atttaacgac
      481 gctcaattta tttatcagtg gcttgccgaa aagtatcctg aagatcagat cgttctttac
      541 ggaaggtcta tgggcagcgg cattgccgcc cgtattgcct catggaacaa gcccaggatg
      601 ttgattctgg attcgcctta tttcagcttt tattataatg tgaaccgcta tttgtttttc
      661 actccgctga agtggttgtt gaagtacgac atgcgcactg accaatacct gaagaccgtc
      721 agttgtccgg tgcacatcat ccacggtaca cgcgaccggt taattaacta tgggcagagc
      781 gaaaagctga aggctttgtt tcctgaaaaa attgttctgc atccgatcaa agacggaaag
      841 cataacaacc tgcctgaatt tccggagttc ttcgaagtgc tctacgatat cctttatgta
      901 aaaccagcag tgacctcaac gtaatggaga tctccagcaa accatccgcc aacttcaccc
      961 ggttatttga cctgatcgac taccagcggg caaggtatcc taacgcacaa gcactaaacg
     1021 cctgcatcaa ttcacagtgg gtagctgaaa gtattgaaac attccgccac cgggtggatt
     1081 caatctccgg ctatcttctc caaaagaatt accaactcgg agagaaaata atcctgatac
     1141 ccgacatcgg ctctgccgac tggatggcca tcgatctggc ttgccaacag atcgggttga
     1201 taccggtgcc cctgcacgct accgtatccg aggccgagtt gactattatt ctggatgaaa
     1261 cacaagcttg cttttgcatc gtgacgttta accaatggga tcgcatcagg aatatcttcg
     1321 aaaagaaaac gaaatctccg gttttgtatc atctgtcggc gaaccatgcg ggatattttt
     1381 tgggggagat taaaggaaat gtttcatcgg atgttctggc gaccattgag aatataaaat
     1441 cgtccattga tgaaaacgat ctggccacca tcatgtatac ctctggcagt tcgggcgaac
     1501 cgaagggtgt catgctttcg catcgcaaca ttgttcacaa cattaagtcg gtgcttactt
//
LOCUS       DAOQXS010000493         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1030
            NODE_103914_length_1518_cov_3.327409, whole genome shotgun
            sequence.
ACCESSION   DAOQXS010000493 DAOQXS010000000
VERSION     DAOQXS010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852373
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,272
            CDSs (with protein)               :: 2,272
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 6 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103914_length_1518_cov_3.327409"
                     /isolate="AMR_MDS_1030"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKM34_11485"
     CDS             complement(<1..571)
                     /locus_tag="PKM34_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ84260.1"
                     /translation="MKTAKLFFFVICILAWAGINETFAQWGVNGSHIYNTNAGNVGIG
                     NNSPTTLLHVQKLMTEPTITIQNLGGTGGATYTMIDNASGANWKFKATLSGGFKIRDH
                     ANSLDVVVIEPNSAANSLYINSSGFIGIGTNAPAQKLTVFGGDFKIDDYYPFIFLNNT
                     STGGNMGITFWQAGEYRAWLYYDDGESLLR"
     gene            complement(761..>1518)
                     /locus_tag="PKM34_11490"
     CDS             complement(761..>1518)
                     /locus_tag="PKM34_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ84261.1"
                     /translation="STVNARSGAKSLISLIITSVILAVSLYFLTGLFKNLPHVILAVI
                     VIDALISLVNLKELKHLFRVSRLEFWVSALTIVAVVLFGVLKGILIAVILSIISVLRK
                     SASPHMAVLGRIPGTTLFSDMLRHPDNQPVDGVLILRPESSLLYFNVNFIRDSIKQAL
                     SNYPGKPYLVILDLSSANFVDVAGARFLLWLEDDLEKKGIGFRIVDALGNVRDTLRKA
                     GMEKEIGHISRKHTINEILAEYNGKPVTEEESF"
BASE COUNT          413 a          357 c          359 g          389 t
ORIGIN      
        1 gtcgcaacaa actctcaccg tcatcatagt ataaccatgc acgatattct ccagcctgcc
       61 agaatgtaat ccccatattt cccccggtac tggtattatt taagaatatg aacgggtaat
      121 aatcgtcaat tttaaaatcg cctccaaata ctgtaagttt ttgagcgggt gcattagtcc
      181 ctattcctat aaatcctgaa ctgttaatat acaatgaatt ggctgcactg ttaggctcta
      241 tgacaaccac atccagtgag ttggcatggt ccctgatttt aaatcccccg ctcagggtag
      301 ctttgaattt ccagttggcg ccgctggcat tgtcgatcat ggtgtaagta gcaccgccgg
      361 taccgcccag gttctgaatg gtaatcgtcg gttcggtcat aagtttctga acatgcagca
      421 gtgtcgtcgg ggaattattc ccgataccaa cattgccggc gttggtgtta taaatatggc
      481 tgccgttaac gccccactgg gcgaaggttt cgttgatacc ggcccatgcc agtatgcaaa
      541 tgacaaaaaa gaaaagtttt gctgttttca taatgatagt atttggttgt tatatcctca
      601 aaatcaatca ttaaagggac ttgcgggggg aattggtcaa tagtacgtaa atatacgtac
      661 aatcggataa aaagtcaagt gaaatcgaga ttttcagaga tattaccagg aactttcagc
      721 gagggaaatt caaatgattt acaatgccag acaataactg ctaaaagctc tcttcctccg
      781 taaccggttt gccattgtat tcagccagta tctcgttaat ggtatgtttc cggctgatat
      841 ggccgatctc tttctccatg cctgctttac gcagggtatc ccgcacatta cccagggcgt
      901 cgacgatccg gaaaccgatc cctttctttt ccaggtcatc ttccagccag aggaggaacc
      961 gggcgccggc cacatcgaca aaattggccg aagagaggtc gaggatgaca agatatggct
     1021 tgccggggta gtttgataac gcttgcttaa tactatccct gataaaattc acattaaagt
     1081 aaagaagtga tgattcgggg cggaggatca gcactccgtc gaccggctga ttatccggat
     1141 ggcgaagcat gtcagaaaaa agtgttgttc cgggtatccg gcccaatacc gccatgtgcg
     1201 gggaggcact tttcctcaaa acggagatga tagacaggat caccgctata agaatccctt
     1261 tcagcacgcc aaagaggaca accgccacga tggtcagcgc cgacacccag aattcaagcc
     1321 ggctcaccct gaacaggtgt ttcaactcct tcaggttgac caggctgatc agcgcgtcga
     1381 tcacgataac ggccaggatc acgtggggca ggtttttaaa caggcccgtc aggaaataga
     1441 ggcttacagc tagtattaca gaggtaataa taagcgaaat caggcttttg gcacccgacc
     1501 gggcattgac cgtggatt
//
